AU2021201261A1 - Single-chain multivalent binding proteins with effector function - Google Patents

Abstract

The present application relates to multivalent binding peptides, including bi-specific binding peptides having immunoglobulin effector function, nucleic acids, vectors, host cells and compositions comprising them. The present application also describes uses of and methods of treating a wide variety of diseases such as cancers, auto immune diseases, inflammation and viral infections etc. using the multivalent binding peptides. Methods of preparation of these peptides are also described.

Description

SINGLE-CHAIN MULTIVALENT BINDING PROTEINS WITH EFFECTOR FUNCTION FIELD OF THE INVENTION

The invention relates generally to the field of multivalent binding molecules and therapeutic applications thereof.

The sequence listing is being submitted as a text file and as a PDF file in compliance with applicable requirements for electronic filing. The sequence listing was created on June 12, 2007. The sequence listing is incorporated herein by reference in its entirety.

BACKGROUND

In a healthy mammal, the immune system protects the body from damage from foreign substances and pathogens. In some instances though, the immune system goes awry, producing traumatic insult and/or disease. For example, B-cells can produce antibodies that recognize self-proteins rather than foreign proteins, leading to the production of the autoantibodies characteristic of autoimmune diseases such as lupus erythematosus, rheumatoid arthritis, and the like. In other instances, the typically beneficial effect of the immune system in combating foreign materials is counterproductive, such as following organ transplantation. The power of the mammalian immune system, and in particular the human immune system, has been recognized and efforts have been made to control the system to avoid or ameliorate the deleterious consequences to health that result either from normal functioning of the immune system in an abnormal environment (e.g., organ transplantation) or from abnormal functioning of the immune system in an otherwise apparently normal environment (e.g., autoimmune disease progression). Additionally, efforts have been made to exploit the immune system to provide a number of target-specific diagnostic and therapeutic methodologies, relying on the capacity of antibodies to specifically recognize and bind antigenic targets with specificity.

One way in which the immune system protects the body is by production of specialized cells called B lymphocytes or B-cells. B-cells produce antibodies that bind to, and in some cases mediate destruction of, a foreign substance or pathogen. In some instances though, the human immune system, and specifically the B lymphocytes of the human immune system, go awry and disease results. There are numerous cancers that involve uncontrolled proliferation of B-cells. There are also numerous autoimmune diseases that involve B-cell production of antibodies that, instead of binding to foreign substances and pathogens, bind to parts of the body. In addition, there are numerous autoimmune and inflammatory diseases that involve B cells in their pathology, for example, through inappropriate B-cell antigen presentation to T-cells or through other pathways involving B-cells. For example, autoimmune-prone mice deficient in B-cells do not develop autoimmune kidney disease, vasculitis or autoantibodies. (Shlomchik et al., J Exp. Med. 1994, 180:1295 306). Interestingly, these same autoimmune-prone mice which possess B-cells but are deficient in immunoglobulin production, do develop autoimmune diseases when induced experimentally (Chan et al., J Exp. Med. 1999, 189:1639-48), indicating that B-cells play an integral role in development of autoimmune disease.

B-cells can be identified by molecules on their cell surface. CD20 was the first human B-cell lineage-specific surface molecule identified by a monoclonal antibody. It is a non-glycosylated, hydrophobic 35 kDa B-cell transmembrane phosphoprotein that has both its amino and carboxy ends situated inside the cell. Einfeld et al., EMBO J. 1988, 7:711-17. CD20 is expressed by all normal mature B cells, but is not expressed by precursor B-cells or plasma cells. Natural ligands for CD20 have not been identified, and the function of CD20 in B-cell biology is still incompletely understood.

Another B-cell lineage-specific cell surface molecule is CD37. CD37 is a heavily glycosylated 40-52 kDa protein that belongs to the tetraspanin transmembrane family of cell surface antigens. It traverses the cell membrane four times forming two extracellular loops and exposing its amino and carboxy ends to the cytoplasm. CD37 is highly expressed on normal antibody-producing (slg+)B-cells, but is not expressed on pre-B-cells or plasma cells. The expression of CD37 on resting and activated T cells, monocytes and granulocytes is low and there is no detectable CD37 expression on NK cells, platelets or erythrocytes. See, Belov et al., Cancer Res., 61(11):4483 4489 (2001); Schwartz-Albiez et al., J. Immunol., 140(3): 905-914 (1988); and Link et al., J. Immunol., 137(9): 3013-3018 (1988). Besides normal B-cells, almost all malignancies of B-cell origin are positive for CD37 expression, including CLL, NHL, and hairy cell leukemia (Moore, et al. 1987; Merson and Brochier 1988; Faure, et al.

1990). CD37 participates in regulation of B-cell function, since mice lacking CD37 were found to have low levels of serum IgG1 and to be impaired in their humoral response to viral antigens and model antigens. It appears to act as a nonclassical costimulatory molecule or by directly influencing antigen presentation via complex formation with MHC class II molecules. See Knobeloch et aL, Mo. Cell. Biol., 20(15):5363-5369 (2000).

Research and drug development has occurred based on the concept that B-cell lineage-specific cell surface molecules such as CD37 and CD20 can themselves be targets for antibodies that would bind to, and mediate destruction of, cancerous and autoimmune disease-causing B-cells that have CD37 and CD20 on their surfaces. Termed "immunotherapy," antibodies made (or based on antibodies made) in a non human animal that bind to CD37 or CD20 were given to a patient to deplete cancerous or autoimmune disease-causing B-cells.

Monoclonal antibody technology and genetic engineering methods have facilitated development of immunoglobulin molecules for diagnosis and treatment of human diseases. The domain structure of immunoglobulins is amenable to engineering, in that the antigen binding domains and the domains conferring effector functions may be exchanged between immunoglobulin classes and subclasses. Immunoglobulin structure and function are reviewed, for example, in Harlow et al., Eds., Antibodies: A Laboratory Manual, Chapter 14, Cold Spring Harbor Laboratory, Cold Spring Harbor (1988). An extensive introduction as well as detailed information about all aspects of recombinant antibody technology can be found in the textbook "Recombinant Antibodies" (John Wiley & Sons, NY, 1999). A comprehensive collection of detailed antibody engineering lab Protocols can be found in R. Kontermann and S. Dibel (eds.), "The Antibody Engineering Lab Manual" (Springer Verlag, Heidelberg/New York, 2000).

An immunoglobulin molecule (abbreviated Ig), is a multimeric protein, typically composed of two identical light chain polypeptides and two identical heavy chain polypeptides (H 2 L2 ) that are joined into a macromolecular complex by interchain disulfide bonds, i.e., covalent bonds between the sulfhydryl groups of neighboring cysteine residues. Five human immunoglobulin classes are defined on the basis of their heavy chain composition, and are named IgG, IgM, IgA, IgE, and

IgD. The IgG-class and IgA-class antibodies are further divided into subclasses, namely, IgG1, IgG2, IgG3, and IgG4, and IgAl and IgA2, respectively. Intrachain disulfide bonds join different areas of the same polypeptide chain, which results in the formation of loops that, along with adjacent amino acids, constitute the immunoglobulin domains. At the amino-terminal portion, each light chain and each heavy chain has a single variable region that shows considerable variation in amino acid composition from one antibody to another. The light chain variable region, VL, has a single antigen-binding domain and associates with the variable region of a heavy chain, VH (also containing a single antigen-binding domain), to form the antigen binding site of the immunoglobulin, the Fv.

In addition to variable regions, each of the fll-length antibody chains has a constant region containing one or more domains. Light chains have a constant region containing a single domain. Thus, light chains have one variable domain and one constant domain. Heavy chains have a constant region containing several domains. The heavy chains in IgG, IgA, and IgD antibodies have three domains, which are designated CHI, CH2, and CH 3 ; the heavy chains in IgM and IgE antibodies have four domains, CHI, CH2 , CH3 and CH 4 . Thus, heavy chains have one variable domain and three or four constant domains. Noteworthy is the invariant organization of these domains in all known species, with the constant regions, containing one or more domains, being located at or near the C-terminus of both the light and heavy chains of immunoglobulin molecules, with the variable domains located towards the N-termini of the light and heavy chains. Immunoglobulin structure and function are reviewed, for example, in Harlow et al., Eds., Antibodies: A LaboratoryManual, Chapter 14, Cold Spring Harbor Laboratory, Cold Spring Harbor (1988).

The heavy chains of immunoglobulins can also be divided into three functional regions: the Fd region (a fragment comprising VH and CHi, i.e., the two N terminal domains of the heavy chain), the hinge region, and the Fc region (the "fragment crystallizable" region). The Fc region contains the domains that interact with immunoglobulin receptors on cells and with the initial elements of the complement cascade. Thus, the Fc region or fragment is generally considered responsible for the effector functions of an immunoglobulin, such as ADCC (antibody-dependent cell-mediated cytotoxicity), CDC (complement-dependent cytotoxicity) and complement fixation, binding to Fc receptors, greater half-life in vivo relative to a polypeptide lacking an Fc region, protein A binding, and perhaps even placental transfer. Capon et al., Nature, 337: 525-531, (1989). Further, a polypeptide containing an Fc region allows for dimerization/multimerization of the polypeptide. These terms are also used for analogous regions of the other immunoglobulins.

Although all of the human immunoglobulin isotypes contain a recognizable structure in common, each isotype exhibits a distinct pattern of effector function. IgG, by way of nonexhaustive example, neutralizes toxins and viruses, opsonizes, fixes complement (CDC) and participates in ADCC. IgM, in contrast, neutralizes blood-borne pathogens and participates in opsonization. IgA, when associated with its secretory piece, is secreted and provides a primary defense to microbial infection via the mucosa; it also neutralizes toxins and supports opsonization. IgE mediates inflammatory responses, being centrally involved in the recruitment of other cells needed to mount a full response. IgD is known to provide an immunoregulatory function, controlling the activation of B cells. These characterizations of isotype effector functions provide a non-comprehensive illustration of the differences that can be found among human isotypes.

The hinge region, found in IgG, IgA, IgD, and IgE class antibodies, acts as a flexible spacer, allowing the Fab portion to move freely in space. In contrast to the constant regions, the hinge domains are structurally diverse, varying in both sequence and length among immunoglobulin classes and subclasses. For example, the length and flexibility of the hinge region varies among the IgG subclasses. The hinge region of IgG1 encompasses amino acids 216-231 and, because it is freely flexible, the Fab fragments can rotate about their axes of symmetry and move within a sphere centered at the first of two inter-heavy chain disulfide bridges. IgG2 has a shorter hinge than IgG1, with 12 amino acid residues and four disulfide bridges. The hinge region of IgG2 lacks a glycine residue, is relatively short, and contains a rigid poly-proline double helix, stabilized by extra inter-heavy chain disulfide bridges. These properties restrict the flexibility of the IgG2 molecule. IgG3 differs from the other subclasses by its unique extended hinge region (about four times as long as the IgG hinge), containing 62 amino acids (including 21 prolines and 11 cysteines), forming an inflexible poly-proline double helix. In IgG3, the Fab fragments are relatively far away from the Fc fragment, giving the molecule a greater flexibility. The elongated hinge in IgG3 is also responsible for its higher molecular weight compared to the other subclasses. The hinge region of IgG4 is shorter than that of IgG Iand its flexibility is intermediate between that of IgG Iand IgG2. The flexibility of the hinge regions reportedly decreases in the order IgG3>IgGl>IgG4>IgG2. The four IgG subclasses also differ from each other with respect to their effector functions. This difference is related to differences in structure, including differences with respect to the interaction between the variable region, Fab fragments, and the constant Fc fragment.

According to crystallographic studies, the immunoglobulin hinge region can be further subdivided functionally into three regions: the upper hinge region, the core region, and the lower hinge region. Shin et al., 1992 ImmunologicalReviews 130:87. The upper hinge region includes amino acids from the carboxyl end of Cm to the first residue in the hinge that restricts motion, generally the first cysteine residue that forms an interchain disulfide bond between the two heavy chains. The length of the upper hinge region correlates with the segmental flexibility of the antibody. The core hinge region contains the inter-heavy chain disulfide bridges, and the lower hinge region joins the amino terminal end of the CH2 domain and includes residues in CH2. Id. The core hinge region of human IgG1 contains the sequence Cys-Pro-Pro-Cys which, when dimerized by disulfide bond formation, results in a cyclic octapeptide believed to act as a pivot, thus conferring flexibility. The hinge region may also contain one or more glycosylation sites, which include a number of structurally distinct types of sites for carbohydrate attachment. For example, IgAl contains five glycosylation sites within a 17-amino-acid segment of the hinge region, conferring resistance of the hinge region polypeptide to intestinal proteases, considered an advantageous property for a secretory immunoglobulin.

Conformational changes permitted by the structure and flexibility of the immunoglobulin hinge region polypeptide sequence may also affect the effector functions of the Fc portion of the antibody. Three general categories of effector functions associated with the Fc region include (1) activation of the classical complement cascade, (2) interaction with effector cells, and (3) compartmentalization of immunoglobulins. The different human IgG subclasses vary in the relative efficacies with which they fix complement, or activate and amplify the steps of the complement cascade. See, e.g., Kirschfink, 2001 Immunol. Rev. 180:177; Chakraborti et al, 2000 Cell Signal 12:607; Kohl et al., 1999 Mol. Immunol. 36:893; Marsh et al., 1999 Curr. Opin. Nephrol. Hypertens. 8:557; Speth et al., 1999 Wien Klin. Wochenschr. 111:378.

Exceptions to the H2L2 structure of conventional antibodies occur in some isotypes of the immunoglobulins found in camelids (camels, dromedaries and llamas; Hamers-Casterman et al., 1993 Nature 363:446; Nguyen et al., 1998 J. Mol. Biol 275:413), nurse sharks (Roux et al., 1998 Proc. Nat. Acad. Sci. USA 95:11804), and in the spotted ratfish (Nguyen, et al., 2002 Immunogenetics 54(1):39-47). These antibodies can apparently form antigen-binding regions using only heavy chain variable region, i.e., these functional antibodies are homodimers of heavy chains only (referred to as "heavy-chain antibodies" or "HCAbs"). Despite the advantages of antibody technology in disease diagnosis and treatment, there are some disadvantageous aspects of developing whole-antibody technologies as diagnostic and/or therapeutic reagents. Whole antibodies are large protein structures exemplified by the heterotetrameric structure of the IgG isotype, containing two light and two heavy chains. Such large molecules are sterically hindered in certain applications. For example, in treatments of solid tumors, whole antibodies do not readily penetrate the interior of the tumor. Moreover, the relatively large size of whole antibodies presents a challenge to ensure that the in vivo administration of such molecules does not induce an immune response. Further, generation of active antibody molecules typically involves the culturing of recombinant eukaryotic cells capable of providing appropriate post-translational processing of the nascent antibody molecules, and such cells can be difficult to culture and difficult to induce in a manner that provides commercially useful yields of active antibody.

Recently, smaller immunoglobulin molecules have been constructed to overcome problems associated with whole immunoglobulin methodologies. A single chain variable antibody fragment (scFv) comprises an antibody heavy chain variable domain joined via a short peptide to an antibody light chain variable domain (Huston et al., Proc. Natl. Acad. Sci. USA, 1988, 85: 5879-83). Because of the small size of scFv molecules, they exhibit more effective penetration into tissues than whole immunoglobulin. An anti-tumor scFv showed more rapid tumor penetration and more even distribution through the tumor mass than the corresponding chimeric antibody (Yokota et al., Cancer Res. 1992, 52:3402-08).

Despite the advantages that scFv molecules bring to serotherapy, several drawbacks to this therapeutic approach exist. An scFv is rapidly cleared from the circulation, which may reduce toxic effects in normal cells, but such rapid clearance impedes delivery of a minimum effective dose to the target tissue. Manufacturing adequate amounts of scFv for administration to patients has been challenging due to difficulties in expression and isolation of scFv that adversely affect the yield. During expression, scFv molecules lack stability and often aggregate due to pairing of variable regions from different molecules. Furthermore, production levels of scFv molecules in mammalian expression systems are low, limiting the potential for efficient manufacturing of scFv molecules for therapy (Davis et al, J Biol. Chem. 1990, 265:10410-18); Traunecker et al., EMBO J 1991, 10: 3655-59). Strategies for improving production have been explored, including addition of glycosylation sites to the variable regions (Jost, C. R. U.S. Pat. No. 5,888,773, Jost et al, J. Biol. Chem. 1994, 69: 26267-73).

Another disadvantage to using scFv for therapy is the lack of effector function. An scFv without a cytolytic function, such as the antibody-dependent cell-mediated cytotoxicity (ADCC) and complement dependent-cytotoxicity (CDC) associated with the constant region of an immunoglobulin, may be ineffective for treating disease. Even though development of scFv technology began over 12 years ago, currently no scFv products are approved for therapy.

Alternatively, it has been proposed that fusion of an scFv to another molecule, such as a toxin, could take advantage of the specific antigen-binding activity and the small size of an scFv to deliver the toxin to a target tissue. Chaudary et al., Nature 1989, 339:394; Batra et al., Mol. Cell. Biol. 1991, 11:2200. Conjugation or fusion of toxins to scFvs has thus been offered as an alternative strategy to provide potent, antigen-specific molecules, but dosing with such conjugates or chimeras can be limited by excessive and/or non-specific toxicity due to the toxin moiety of such preparations. Toxic effects may include supraphysiological elevation of liver enzymes and vascular leak syndrome, and other undesired effects. In addition, immunotoxins are themselves highly immunogenic upon administration to a host, and host antibodies generated against the immunotoxin limit potential usefulness for repeated therapeutic treatments of an individual.

Nonsurgical cancer therapy, such as external irradiation and chemotherapy, can suffer from limited efficacy because of toxic effects on normal tissues and cells, due to the lack of specificity these treatments exhibit towards cancer cells. To overcome this limitation, targeted treatment methodologies have been developed to increase the specificity of the treatment for the cells and tissues in need thereof. An example of such a targeted methodology for in vivo use is the administration of antibody conjugates, with the antibody designed to specifically recognize a marker associated with a cell or tissue in need of treatment, and the antibody being conjugated to a therapeutic agent, such as a toxin in the case of cancer treatment. Antibodies, as systemic agents, circulate to sensitive and undesirable body compartments, such as the bone marrow. In acute radiation injury, destruction of lymphoid and hematopoietic compartments is a major factor in the development of septicemia and subsequent death. Moreover, antibodies are large, globular proteins that can exhibit poor penetration of tissues in need of treatment.

Human patients and non-human subjects suffering from a variety of end-stage disease processes frequently require organ transplantation. Organ transplantation, however, must contend with the untoward immune response of the recipient and guard against immunological rejection of the transplanted organ by depressing the recipient's cellular immune response to the foreign organ with cytotoxic agents which affect the lymphoid and other parts of the hematopoietic system. Graft acceptance is limited by the tolerance of the recipient to these cytotoxic chemicals, many of which are similar to the anticancer (antiproliferative) agents. Likewise, when using cytotoxic antimicrobial agents, particularly antiviral drugs, or when using cytotoxic drugs for autoimmune disease therapy, e.g., in treatment of systemic lupus erythematosis, a serious limitation is the toxic effects of the therapeutic agents on the bone marrow and the hematopoietic cells of the body.

Use of targeted therapies, such as targeted antibody conjugate therapy, is designed to localize a maximum quantity of the therapeutic agent at the site of desired action as possible, and the success of such therapies is revealed by the relatively high signal-to-background ratio of therapeutic agent. Examples of targeted antibodies include diagnostic or therapeutic agent conjugates of antibody or antibody fragments, cell-or tissue-specific peptides, and hormones and other receptor-binding molecules. For example, antibodies against different determinants associated with pathological and normal cells, as well as associated with pathogenic microorganisms, have been used for the detection and treatment of a wide variety of pathological conditions or lesions. In these methods, the targeting antibody is directly conjugated to an appropriate detecting or therapeutic agent as described, for example, in Hansen et at., U.S. Pat. No. 3,927,193 and Goldenberg, U.S. Pat. Nos. 4,331,647, 4,348,376, 4,361,544, 4,468,457, 4,444,744, 4,460,459, 4,460,561, 4,624,846 and 4,818,709,

One problem encountered in direct targeting methods, i.e., in methods wherein the diagnostic or therapeutic agent (the "active agent") is conjugated directly to the targeting moiety, is that a relatively small fraction of the conjugate actually binds to the target site, while the majority of conjugate remains in circulation and compromises in one way or another the function of the targeted conjugate. To ensure maximal localization of the active agent, an excess of the targeted conjugate is typically administered, ensuring that some conjugate will remain unbound and contribute to background levels of the active agent. A diagnostic conjugate, e.g., a radioimmunoscintigraphic or magnetic resonance imaging conjugate that does not bind its target can remain in circulation, thereby increasing background and decreasing resolution of the diagnostic technique. In the case of a therapeutic conjugate having a toxin as an active agent (e.g., a radioisotope, drug or toxic compound) attached to a long-circulating targeting moiety such as an antibody, circulating conjugate can result in unacceptable toxicity to the host, such as marrow toxicity or systemic side effects.

U.S. Pat. No. 4,782,840 discloses a method for reducing the effect of elevated background radiation levels during surgery. The method involves injection of a patient with antibodies specific for neoplastic tissue, with the antibodies labeled with radioisotopes having a suitably long half-life, such as Iodine-125. After injection of the radiolabeled antibody, the surgery is delayed at least 7-10 days, preferably 14-21 days, to allow any unbound radiolabeled antibody to be cleared to a low background level.

U.S. Pat. No. 4,932,412 discloses methods for reducing or correcting for non specific background radiation during intraoperative detection. The methods include the administration to a patient who has received a radiolabeled primary antibody, of a contrast agent, subtraction agent or second antibody which binds the primary antibody.

Apart from producing the antibodies described above, the immune system includes a variety of cell types that have powerful biological effects. During hematopoiesis, bone marrow-derived stem cells differentiate into either mature cells of the immune system ("B" cells) or into precursors of cells that migrate out of the bone marrow to mature in the thymus ("T" cells).

B cells are central to the humoral component of an immune response. B cells are activated by an appropriate presentation of an antigen to become antibody secreting plasma cells; antigen presentation also results in clonal expansion of the activated B celL B cells are primarily responsible for the humoral component of an immune response. A plasma cell typically exhibits about 105 antibody molecules (IgD and IgM) on its surface.

T lymphocytes can be divided into two categories. The cytotoxic T cells, Tc lymphocytes or CTLs (CD8+ T cells), kill cells bearing foreign surface antigen in association with Class I MHC and can kill cells that are harboring intracellular parasites (either bacteria or viruses) as long as the infected cell is displaying a microbial antigen on its surface. Tc cells kill tumor cells and account for the rejection of transplanted cells. Tc cells recognize antigen-Class I MHC complexes on target cells, contact them, and release the contents of granules directly into the target cell membrane, which lyses the cell.

A second category of T cells is the helper T cell or Th lymphocyte (CD4+ T cells), which produces lymphokines that are "helper" factors in the maturation of B cells into antibody-secreting plasma cells. Th cells also produce certain lymphokines that stimulate the differentiation of effector T lymphocytes and the activity of macrophages. Thi cells recognize antigen on macrophages in association with Class

II MHC and become activated (by IL-i) to produce lymphokines, including the IFN-y that activates macrophages and NK cells. These cells mediate various aspects of the cell-mediated immunity response including delayed-type hypersensitivity reactions. Th2 cells recognize antigen in association with Class II MHC on an antigen presenting cell or APC (e.g., migratory macrophages and dendritic cells) and then produce interleukins and other substances that stimulate specific B-cell and T-cell proliferation and activity.

Beyond serving as APCs that initiate T cell interactions, development, and proliferation, macrophages are involved in expression of cell-mediated immunity because they become activated by IFN-y produced in a cell-mediated immune response. Activated macrophages have increased phagocytic potential and release soluble substances that cause inflammation and destroy many bacteria and other cells. Natural Killer cells are cytotoxic cells that lyse cells bearing new antigen, regardless of their MHC type, and even lyse some cells that bear no MHC proteins. Natural Killer T cells, or NK cells, are defined by their ability to kill cells displaying a foreign antigen (e.g., tumor cells), regardless of MHC type, and regardless of previous sensitization (exposure) to the antigen. NK cells can be activated by IL-2 and IFN-y, and lyse cells in the same manner as cytotoxic T lymphocytes. Some NK cells have receptors for the Fc domain of the IgG antibody (e.g, CD16 or FCyRIII) and are thus able to bind to the Fe portion of IgG on the surface of a target cell and release cytolytic components that kill the target cell via antibody-dependent cell-mediated cytotoxicity.

Another group of cells is the granulocytes or polymorphonuclear leukocytes (PMNs). Neutrophils, one type of PMN, kill bacterial invaders and phagocytose the remains. Eosinophils are another type of PMN and contain granules that prove cytotoxic when released upon another cell, such as a foreign cell. Basophils, a third type of PMN, are significant mediators of powerful physiological responses (e.g., inflammation) that exert their effects by releasing a variety of biologically active compounds, such as histamine, serotonin, prostaglandins, and leukotrienes. Common to all of these cell types is the capacity to exert a physiological effect within an organism, frequently by killing, and optionally scavenging, deleterious compositions such as foreign cells.

Although a variety of mammalian cells, including cells of the immune system, are capable of directly exerting a physiological effect (e.g., cell killing, typified by Tc, NK, some PMN, macrophage, and the like), other cells indirectly contribute to a physiological effect. For example, initial presentation of an antigen to a naive T cell of the immune system requires MHC presentation that mandates cell-cell contact. Further, there often needs to be contact between an activated T cell and an antigen specific B cell to obtain a particular immunogenic response. A third form of cell-cell contact often seen in immune responses is the contact between an activated B cell and follicular dendritic cells. Each of these cell-cell contact requirements complicates the targeting of a biologically active agent to a given target.

Complement-dependent cytotoxicity (CDC) is believed to be a significant mechanism for clearance of specific target cells such as tumor cells. CDC is a series of events that consists of a collection of enzymes that become activated by each other in a cascade fashion. Complement has an important role in clearing antigen, accomplished by its four major functions: (1) local vasodilation; (2) attraction of immune cells, especially phagocytes (chemotaxis); (3) tagging of foreign organisms for phagocytosis (opsonization); and (4) destruction of invading organisms by the membrane attack complex (MAC attack). The central molecule is the C3 protein. It is an enzyme that is split into two fragments by components of either the classical pathway or the alternative pathway. The classical pathway is induced by antibodies, especially IgG and IgM, while the alternative pathway is nonspecifically stimulated by bacterial products like lipopolysaccharide (LPS). Briefly, the products of the C3 split include a small peptide C3a which is chemotactic for phagocytic immune cells and results in local vasodilation by causing the release of C5a fragment from C5. The other part of C3, C3b, coats antigens on the surface of foreign organisms and acts to opsonize the organism for destruction. C3b also reacts with other components of the complement system to form an MAC consisting of C5b, C6, C7, C8 and C9.

There are problems associated with the use of antibodies in human therapy because the response of the immune system to any antigen, even the simplest, is "polyclonal," i.e., the system manufactures antibodies of a great range of structures both in their binding regions as well as in their effector regions.

Two approaches have been used in an attempt to reduce the problem of immunogenic antibodies. The first is the production of chimeric antibodies in which the antigen-binding part (variable regions) of a mouse monoclonal antibody is fused to the effector part (constant region) of a human antibody. In a second approach, antibodies have been altered through a technique known as complementarity determining region (CDR) grafting or "humanization." This process has been further improved to include changes referred to as "reshaping" (Verhoeyen, et al., 1988 Science 239:1534-1536; Riechmann, et al., 1988 Nature 332:323-337; Tempest, et al., Bio/fechnol 19919:266-271), "hyperchimerization" (Queen, et al., 1989 Proc Natl Acad Sci USA 86:10029-10033; Co, et al., 1991 ProcNatl Acad Sci USA 88:2869 2873; Co, et al., 1992 JImnunol 148:1149-1154), and "veneering" (Mark, et al., In: Metcalf BW, Dalton BJ, eds. Cellular adhesion: molecular definition to therapeutic potential. New York: Plenum Press, 1994:291-312).

An average of less than one therapeutic antibody per year has been introduced to the market beginning in 1986, eleven years after the publication of monoclonal antibodies. Five murine monoclonal antibodies were introduced into human medicine over a ten year period from 1986-1995, including "muromonab-CD3" (OrthoClone OKT3@) for acute rejection of organ transplants; "edrecolomab" (Panorex@) for colorectal cancer; "odulimomab" (Antilfa@) for transplant rejection; and, "ibritumomab" (Zevalin@ yiuxetan) for non-Hodgkin's lymphoma. Additionally, a monoclonal Fab, "abciximab" (ReoPro@) has been marketed for preventing coronary artery reocclusion. Three chimeric monoclonal antibodies were also launched: "rituximab" (Rituxan@) for treating B cell lymphomas; "basiliximab" (Simulect@)for transplant rejection; and "infliximab" (Remicade®) for treatment of rheumatoid arthritis and Crohn's disease. Additionally, "abciximab" (ReoPro@), a 47.6 kD Fab fragment of a chimeric human-murine monoclonal antibody is marketed as an adjunct to percutaneous coronary intervention for the prevention of cardiac ischemic complications in patients undergoing percutaneous coronary intervention. Finally, seven "humanized" monoclonal antibodies have been launched. "Daclizumab" (Zenapax@) is used to prevent acute rejection of transplanted kidneys; "pativizumab" (Synagis®) for RSV; "trastuzumab" (Herceptin®) binds HER-2, a growth factor receptor found on breast cancers cells; "gemtuzumab" (Mylotarg®) for acute myelogenous leukemia (AML); and "alemtuzumab" (MabCampath®) for chronic lymphocytic leukemia; "adalimumab" (Humira@ (D2E7)) for the treatment of rheumatoid arthritis; and, "omalizumab" (Xolair), for the treatment of persistent asthma.

Thus, a variety of antibody technologies have received attention in the effort to develop and market more effective therapeutics and palliatives. Unfortunately, problems continue to compromise the promise of each of these therapies. For example, the majority of cancer patients treated with rituximab relapse, generally within about 6-12 months, and fatal infusion reactions within 24 hours of rituximab infusion have been reported. Acute renal failure requiring dialysis with instances of fatal outcome has also been reported in treatments with rituximab, as have severe, occasionally fatal, mucocutaneous reactions. Additionally, high doses of rituximab are required for intravenous injection because the molecule is large, approximately 150 kDa, and diffusion into the lymphoid tissues, where many tumor cells may reside is limited.

Trastuzumab administration can result in the development of ventricular dysfunction, congestive heart failure, and severe hypersensitivity reactions (including anaphylaxis), infusion reactions, and pulmonary events. Daclizumab immunosuppressive therapy poses an increased risk for developing lymphoproliferative disorders and opportunistic infections. Death from liver failure, arising from severe hepatotoxicity, and from veno-occlusive disease (VOD), has been reported in patients who received gemtuzumab.

Hepatotoxicity was also reported in patients receiving alemtuzumab. Serious and, in some rare instances fatal, pancytopenia/marrow hypoplasia, autoimmune idiopathic thrombocytopenia, and autoimmune hemolytic anemia have occurred in patients receiving alemtuzumab therapy. Alemtuzumab can also result in serious infusion reactions as well as opportunistic infections. In patients treated with adalimumab, serious infections and sepsis, including fatalities, have been reported, as has the exacerbation of clinical symptoms and/or radiographic evidence of demyelinating disease, and patients treated with adalimumab in clinical trials had a higher incidence of lymphoma than the expected rate in the general population. Omalizumab reportedly induces malignancies and anaphylaxis.

Cancer includes a broad range of diseases, affecting approximately one in four individuals worldwide. Rapid and unregulated proliferation of malignant cells is a hallmark of many types of cancer, including hematological malignancies. Although patients with a hematologic malignant condition have benefited from advances in cancer therapy in the past two decades, Multani et al., 1998 J. Clin. Oncology 16:3691-3710, and remission times have increased, most patients still relapse and succumb to their disease. Barriers to cure with cytotoxic drugs include, for example, tumor cell resistance and the high toxicity of chemotherapy, which prevents optimal dosing in many patients.

Treatment of patients with low grade or follicular B cell lymphoma using a chimeric CD20 monoclonal antibody has been reported to induce partial or complete responses in patients. McLaughlin et al., 1996 Blood 88:90a (abstract, suppl. 1); Maloney et al., 1997 Blood 90:2188-95. However, as noted above, tumor relapse commonly occurs within six months to one year, Further improvements in serotherapy are needed to induce more durable responses, for example, in low grade B cell lymphoma, and to allow effective treatment of high grade lymphoma and other B cell diseases.

Another approach has been to target radioisotopes to B cell lymphomas using monoclonal antibodies specific for CD20. While the effectiveness of therapy is reportedly increased, associated toxicity from the long in vivo half-life of the radioactive antibody increases, sometimes requiring that the patient undergo stem cell rescue. Press et al., 1993 N. Eng. J. Med. 329:1219-1224; Kaminski et al., 1993 N. Eng.J Med. 329:459-65. Monoclonal antibodies to CD20 have also been cleaved with proteases to yield F(ab') 2 or Fab fragments prior to attachment of radioisotope. This has been reported to improve penetration of the radioisotope conjugate into the tumor and to shorten the in vivo half-life, thus reducing the toxicity to normal tissues. However, these molecules lack effector functions, including complement fixation and/or ADCC.

Autoimmune diseases include autoimmune thyroid diseases, which include Graves'disease and Hashimoto's thyroiditis. In the United States alone, there are about 20 million people who have some form of autoimmune thyroid disease. Autoimmune thyroid disease results from the production of autoantibodies that either stimulate the thyroid to cause hyperthyroidism (Graves' disease) or destroy the thyroid to cause hypothyroidism (Hashimoto's thyroiditis). Stimulation of the thyroid is caused by autoantibodies that bind and activate the thyroid stimulating hormone (TSH) receptor. Destruction of the thyroid is caused by autoantibodies that react with other thyroid antigens. Current therapy for Graves' disease includes surgery, radioactive iodine, or antithyroid drug therapy. Radioactive iodine is widely used, since antithyroid medications have significant side effects and disease recurrence is high. Surgery is reserved for patients with large goiters or where there is a need for very rapid normalization of thyroid function. There are no therapies that target the production of autoantibodies responsible for stimulating the TSH receptor. Current therapy for Hashimoto's thyroiditis is levothyroxine sodium, and lifetime therapy is expected because of the low likelihood of remission. Suppressive therapy has been shown to shrink goiters in Hashimoto's thyroiditis, but no therapies that reduce autoantibody production to target the disease mechanism are known.

Rheumatoid arthritis (RA) is a chronic disease characterized by inflammation of the joints, leading to swelling, pain, and loss of function. RA affects an estimated 2.5 million people in the United States. RA is caused by a combination of events including an initial infection or injury, an abnormal immune response, and genetic factors. While autoreactive T cells and B cells are present in RA, the detection of high levels of antibodies that collect in the joints, called rheumatoid factor, is used in the diagnosis of RA. Current therapy for RA includes many medications for managing pain and slowing the progression of the disease. No therapy has been found that can cure the disease. Medications include nonsteroidal anti-inflammatory drugs (NSAIDS), and disease modifying anti-rheumatic drugs (DMARDS). NSAIDS are useful in benign disease, but fail to prevent the progression to joint destruction and debility in severe RA. Both NSAIDS and DMARDS are associated with significant side effects. Only one new DMARD, Leflunomide, has been approved in over 10 years. Leflunomide blocks production of autoantibodies, reduces inflammation, and slows progression of RA. However, this drug also causes severe side effects including nausea, diarrhea, hair loss, rash, and liver injury.

Systemic Lupus Erythematosus (SLE) is an autoimmune disease caused by recurrent injuries to blood vessels in multiple organs, including the kidney, skin, and joints. SLE is estimated to affect over 500,000 people in the United States. In patients with SLE, a faulty interaction between T cells and B cells results in the production of autoantibodies that attack the cell nucleus. These include anti-double stranded DNA and anti-Sm antibodies. Autoantibodies that bind phospholipids are also found in about half of SLE patients, and are responsible for blood vessel damage and low blood counts. Immune complexes accumulate in the kidneys, blood vessels, and joints of SLE patients, where they cause inflammation and tissue damage. No treatment for SLE has been found to cure the disease. NSAIDS and DMARDS are used for therapy depending upon the severity of the disease. Plasmapheresis with plasma exchange to remove autoantibodies can cause temporary improvement in SLE patients. There is general agreement that autoantibodies are responsible for SLE, so new therapies that deplete the B cell lineage, allowing the immune system to reset as new B cells are generated from precursors, would offer hope for long lasting benefit in SLE patients.

Sjogren's syndrome is an autoimmune disease characterized by destruction of the body's moisture-producing glands. Sjogren's syndrome is one of the most prevalent autoimmune disorders, striking up to an estimated 4 million people in the United States. About half of the people stricken with Sjogren's syndrome also have a connective tissue disease, such as RA, while the other half have primary Sjogren's syndrome with no other concurrent autoimmune disease. Autoantibodies, including anti-nuclear antibodies, rheumatoid factor, anti-fodrin, and anti-muscarinic receptor are often present in patients with Sjogren's syndrome. Conventional therapy includes corticosteroids, and additional more effective therapies would be of benefit.

Immune thrombocytopenic purpura (ITP) is caused by autoantibodies that bind to blood platelets and cause their destruction. Some cases of ITP are caused by drugs, and others are associated with infection, pregnancy, or autoimmune disease such as SLE. About half of all cases are classified as being of idiopathic origin. The treatment of ITP is determined by the severity of the symptoms. In some cases, no therapy is needed although in most cases immunosuppressive drugs, including corticosteroids or intravenous infusions of immune globulin to deplete T cells, are provided. Another treatment that usually results in an increased number of platelets is removal of the spleen, the organ that destroys antibody-coated platelets. More potent immunosuppressive drugs, including cyclosporine, cyclophosphamide, or azathioprine are used for patients with severe cases. Removal of autoantibodies by passage of patients' plasma over a Protein A column is used as a second line treatment in patients with severe disease. Additional more effective therapies are needed.

Multiple sclerosis (MS) is also an autoimmune disease. It is characterized by inflammation of the central nervous system and destruction of myelin, which insulates nerve cell fibers in the brain, spinal cord, and body. Although the cause of MS is unknown, it is widely believed that autoimmune T cells are primary contributors to the pathogenesis of the disease. However, high levels of antibodies are present in the cerebrospinal fluid of patients with MS, and some predict that the B cell response leading to antibody production is important for mediating the disease. No B cell depletion therapies have been studied in patients with MS, and there is no cure for MS. Current therapy is corticosteroids, which can reduce the duration and severity of attacks, but do not affect the course of MS over time. New biotechnology interferon (IFN) therapies for MS have recently been approved but additional more effective therapies are required.

Myasthenia Gravis (MG) is a chronic autoimmune neuromuscular disorder that is characterized by weakness of the voluntary muscle groups. MG affects about 40,000 people in the United States. MG is caused by autoantibodies that bind to acetylcholine receptors expressed at neuromuscular junctions. The autoantibodies reduce or block acetylcholine receptors, preventing the transmission of signals from nerves to muscles. There is no known cure for mg. Common treatments include immunosuppression with corticosteroids, cyclosporine, cyclophosphamide, or azathioprine. Surgical removal of the thymus is often used to blunt the autoimmune response. Plasmapheresis, used to reduce autoantibody levels in the blood, is effective in mg, but is short-lived because the production of autoantibodies continues. Plasmapheresis is usually reserved for severe muscle weakness prior to surgery. New and effective therapies would be of benefit.

Psoriasis affects approximately five million people, and is characterized by autoimmune inflammation in the skin. Psoriasis is also associated with arthritis in 30% (psoriatic arthritis). Many treatments, including steroids, uv light retinoids, vitamin D derivatives, cyclosporine, and methotrexate have been used but it is also clear that psoriasis would benefit from new and effective therapies. Scleroderma is a chronic autoimmune disease of the connective tissue that is also known as systemic sclerosis. Scleroderma is characterized by an overproduction of collagen, resulting in a thickening of the skin, and approximately 300,000 people in the United States have scleroderma, which would also benefit from new and effective therapies.

Apparent from the foregoing discussion are needs for improved compositions and methods to treat, ameliorate or prevent a variety of diseases, disorders and conditions, including cancer and autoimmune diseases.

SUMMARY

It is to be understood that, if any prior art publication is referred to herein, such reference does not constitute an admission that the publication forms a part of the common general knowledge in the art, in Australia or any other country.

In the claims which follow and in the preceding description of the invention, except where the context requires otherwise due to express language or necessary implication, the word "comprise" or variations such as "comprises" or "comprising" is used in an inclusive sense, i.e. to specify the presence of the stated features but not to preclude the presence or addition of further features in various embodiments of the invention.

The invention satisfies at least one of the aforementioned needs in the art by providing proteins containing at least two specific binding domains, wherein those two domains are linked by a constant sub-region derived from an antibody molecule attached at its C-terminus to a linker herein referred to as a scorpion linker, and nucleic acids encoding such proteins, as well as production, diagnostic and therapeutic uses of such proteins and nucleic acids. The constant sub-region comprises a domain derived from an immunoglobulin CH2 domain, and preferably a domain derived from an immunoglobulin CH3 domain, but does not contain a domain or region derived from, or corresponding to, an immunoglobulin CHI domain. Previously, it had been thought that the placement of a constant region derived from an antibody in the interior of a protein would interfere with antibody function, such as effector function, by analogy to the conventional placement of constant regions of antibodies at the carboxy termini of antibody chains. In addition, placement of a scorpion linker, which may be an immunoglobulin hinge-like peptide, C terminal to a constant sub-region is an organization that differs from the organization of naturally occurring immunoglobulins. Placement of a constant sub-region (with a scorpion linker attached C-terminal to the constant region) in the interior of a polypeptide or protein chain in accordance with the invention, however, resulted in proteins exhibiting effector function and multivalent (mono- or multi-specific) binding capacities relatively unencumbered by steric hindrances. As will be apparent to one of skill in the art upon consideration of this disclosure, such proteins are modular in design and may be constructed by selecting any of a variety of binding domains for binding domain I or binding domain 2 (or for any additional binding domains found in a particular protein according to the invention), by selecting a constant sub-region having effector function, and by selecting a scorpion linker, hinge-like or non-hinge like (e.g., type II C-lectin receptor stalk region peptides), with the protein exhibiting a general organization of N binding domain I-constant sub-region-scorpion linker- binding domain 2-C. Those of skill will further appreciate that proteins of such structure, and the nucleic acids encoding those proteins, will find a wide variety of applications, including medical and veterinary applications.

One aspect of the invention provides a single-chain binding protein comprising from amino-terminus to carboxy-terminus: (a) a first binding domain comprising variable regions from an immunoglobulin or immunoglobulin-like molecule; (b) an immunoglobulin constant sub-region that comprises immunoglobulin CH2 and CH3 domains; (c) a scorpion linker peptide, wherein said scorpion linker peptide comprises an amino acid sequence derived from a hinge of an immunoglobulin or a stalk region of a type II C-lectin protein; and (d) a second binding domain comprising variable regions from an immunoglobulin or immunoglobulin-like molecule.

One further aspect of the invention is drawn to a multivalent single-chain binding protein with effector function, or scorpion (the terms are used interchangeably), comprising a first binding domain derived from an immunoglobulin (e.g., an antibody) or an immunoglobulin-like molecule, a constant sub-region providing an effector function, the constant sub-region located C-terminal to the first binding domain; a scorpion linker located C-terminal to the constant sub-region; and a second binding domain derived from an immunoglobulin (such as an antibody) or immunoglobulin-like molecule, located C terminal to the constant sub-region; thereby localizing the constant sub-region between the first binding domain and the second binding domain. The single-chain binding protein may be multispecific, e.g., bispecific in that it could bind two or more distinct targets, or it may be monospecific, with two binding sites for the same target. Moreover, all of the domains of the protein are found in a single chain, but the protein may form homo-multimers, e.g., by interchain disulfide bond formation. In some embodiments, the first binding domain and/or the second binding domain is/are derived from variable regions of light and heavy

21a

immunoglobulin chains from the same, or different, immunoglobulins (e.g., antibodies). The immunoglobulin(s) may be from any vertebrate, such as a mammal, including a human, and may be chimeric, humanized, fragments, variants or derivatives of naturally occurring immunoglobulins.

The invention contemplates proteins in which the first and second binding domains are derived from the same, or different immunoglobulins (e.g., antibodies), and wherein the first and second binding domains recognize the same, or different, molecular targets (e.g., cell surface markers, such as membrane-bound proteins). Further, the first and second binding domains may recognize the same, or different, epitopes. The first and second molecular targets may be associated with first and second target cells, viruses, carriers and/or objects. In preferred embodiments according to this aspect of the invention, each of the first binding domain, second binding domain, and constant sub-region is derived from a human immunoglobulin, such as an IgG antibody. In yet other embodiments, the multivalent binding protein with effector function has at least one of the first binding domain and the second binding domain that recognizes at least one cell-free molecular target, e.g., a protein unassociated with a cell, such as a deposited protein or a soluble protein. Cell-free molecular targets include, e.g., proteins that were never associated with a cell, e.g., administered compounds such as proteins, as well as proteins that are secreted, cleaved, present in exosomes, or otherwise discharged or separated from a cell.

The target molecules recognized by the first and second binding domains may be found on, or in association with, the same, or different, prokaryotic cells, eukaryotic cells, viruses (including bacteriophage), organic or inorganic target molecule carriers, and foreign objects. Moreover, those target molecules may be on physically distinct cells, viruses, carriers or objects of the same type (e.g., two distinct eukaryotic cells, prokaryotic cells, viruses or carriers) or those target molecules may be on cells, viruses, carriers, or objects that differ in type (e.g., a eukaryotic cell and a virus). Target cells are those cells associated with a target molecule recognized by a binding domain and includes endogenous or autologous cells as well as exogenous or foreign cells (e.g., infectious microbial cells, transplanted mammalian cells including transfused blood cells). The invention comprehends targets for the first and/or second binding domains that are found on the surface of a target cell(s) associated with a disease, disorder or condition of a mammal such as a human. Exemplary target cells include a cancer cell, a cell associated with an autoimmune disease or disorder, and an infectious cell (e.g., an infectious bacterium). A cell of an infectious organism, such as a mammalian parasite, is also contemplated as a target cell. In some embodiments, a protein of the invention is a multivalent (e.g., multispecific) binding protein with effector function wherein at least one of the first binding domain and the second binding domain recognizes a target selected from the group consisting of a tumor antigen, a B-cell target, a TNF receptor superfamily member, a Hedgehog family member, a receptor tyrosine kinase, a proteoglycan-related molecule, a TGF-beta superfamily member, a Wnt-related molecule, a receptor ligand, a T-cell target, a

Dendritic cell target, an NK cell target, a monocyte/macrophage cell target and an angiogenesis target.

In some embodiments of the above-described protein, the tumor antigen is selected from the group consisting of SQUAMOUS CELL CARCINOMA ANTIGEN 1 (SCCA-1), (PROTEIN T4-A), SQUAMOUS CELL CARCINOMA ANTIGEN 2 (SCCA-2), Ovarian carcinoma antigen CA125 (Al-3B) (KIAA0049), MUCIN 1 (TUMOR-ASSOCIATED MUCIN), (CARCINOMA-ASSOCIATED MUCIN), (POLYMORPHIC EPITHELIAL MUCIN),(PEM),(PEMT),(EPISIALIN), (TUMOR-ASSOCIATED EPITHELIAL MEMBRANE ANTIGEN),(EMA),(H23AG), (PEANUT-REACTIVE URINARY MUCIN), (PUM), (BREAST CARCINOMA- ASSOCIATED ANTIGEN DF3), CTCL tumor antigen sel-1, CTCL tumor antigen sel4-3, CTCL tumor antigen se20-4, CTCL tumor antigen se20-9, CTCL tumor antigen se33-1, CTCL tumor antigen se37-2, CTCL tumor antigen se57-1, CTCL tumor antigen se89-1, Prostate-specific membrane antigen, 5T4 oncofetal trophoblast glycoprotein, Orf73 Kaposi's sarcoma-associated herpesvirus, MAGE-Ci (cancer/testis antigen CT7), MAGE-Bi ANTIGEN (MAGE XP ANTIGEN) (DAM10), MAGE-B2 ANTIGEN (DAM6), MAGE-2 ANTIGEN, MAGE-4a antigen, MAGE-4b antigen, Colon cancer antigen NY-CO-45, Lung cancer antigen NY-LU-12 variant A, Cancer associated surface antigen, Adenocarcinoma antigen ART1, Paraneoplastic associated brain-testis-cancer antigen (onconeuronal antigen MA2; paraneoplastic neuronal antigen), Neuro-oncological ventral antigen 2 (NOVA2), Hepatocellular carcinoma antigen gene 520, TUMOR-ASSOCIATED ANTIGEN CO-029, Tumor-associated antigen MAGE-X2, Synovial sarcoma, X breakpoint 2, Squamous cell carcinoma antigen recognized by T cell, Serologically defined colon cancer antigen 1, Serologically defined breast cancer antigen NY-BR 15, Serologically defined breast cancer antigen NY-BR-I6, Chromogranin A; parathyroid secretory protein 1, DUPAN-2, CA 19-9, CA 72-4, CA 195 and L6.

Embodiments of the above-described method comprise a B cell target selected from the group consisting of CD10, CD19, CD20, CD21, CD22, CD23, CD24, CD37, CD38, CD39, CD40, CD72, CD73, CD74, CDw75, CDw76, CD77, CD78, CD79a/b, CD80, CD81, CD82, CD83, CD84, CD85, CD86, CD89, CD98, CD126, CD127, CDwl30, CD138 and CDw150.

In other embodiments of the above-described method, the TNF receptor superfamily member is selected from the group consisting of 4-1BB/TNFRSF9, NGF R/TNFRSF16, BAFF RJTNFRSF13C, Osteoprotegerin/TNFRSF11B, BCMA/TNFRSF17, OX40/TNFRSF4, CD27/TNFRSF7, RANK/TNFRSF1IA, CD30/TNFRSF8, RELT/TNFRSF19L, CD40/TNFRSF5, TACI/TNFRSF13B, DcR3/TNFRSF6B, TNF RI/TNFRSF1A, DcTRAIL R1I/TNFRSF23, TNF RII/TNFRSF1B, DcTRAIL R2/TNFRSF22, TRAIL R1/TNFRSF1OA, DR3/TNFRSF25, TRAIL R2/TNFRSFIOB, DR6/TNFRSF21, TRAIL R3/TNFRSF1OC, EDAR, TRAIL R4/TNFRSF1OD, Fas/TNFRSF6, TROY/TNFRSF19, GITR/TNFRSF18, TWEAK R/TNFRSF12, HVEM/TNFRSF14, XEDAR, Lymphotoxin beta R/TNFRSF3, 4-1BB Ligand/TNFSF9, Lymphotoxin, APRIL/TNFSF13, Lymphotoxin beta/TNFSF3, BAFF/TNFSF13C, OX40 Ligand/TNFSF4, CD27 Ligand/TNFSF7, TLIA/TNFSF15, CD30 Ligand/TNFSF8, TNF-alpha/TNFSF1A, CD40 Ligand/TNFSF5, TNF-beta/TNFSF1B, EDA-A2, TRAIL/TNFSF10, Fas Ligand/TNFSF6, TRANCE/TNFSF11, GITR Ligand/TNFSF18, TWEAK/TNFSF12 and LIGHT/TNFSF14.

The above-described method also includes embodiments in which the Hedgehog family member is selected from the group consisting of Patched and Smoothened. In yet other embodiments, the proteoglycan-related molecule is selected from the group consisting of proteoglycans and regulators thereof.

Additional embodiments of the method are drawn to processes in which the receptor tyrosine kinase is selected from the group consisting of Ax, FGF R4, Clq R1/CD93, FGF R5, DDRI, Flt-3, DDR2, HGF R, Dtk, IGF-I R, EGF R, IGF-II R, Eph, INSRR, EphA1, Insulin R/CD220, EphA2, M-CSF R, EphA3, Mer, EphA4, MSP R/Ron, EphA5, MuSK, EphA6, PDGF R alpha, EphA7, PDGF R beta, EphA8, Ret, EphB1, RORi, EphB2, ROR2, EphB3, SCF R/c-kit, EphB4, Tie-1, EphB6, Tie 2, ErbB2, TrkA, ErbB3, TrkB, ErbB4, TrkC, FGF R1, VEGF R1I/Flt-1, FGF R2, VEGF R2/Flk-1, FGF R3 and VEGF R3/Flt-4.

In other embodiments of the method, the Transforming Growth Factor (TGF) beta superfamily member is selected from the group consisting of Activin RIA/ALK 2, GFR alpha-1, Activin RIB/ALK-4, GFR alpha-2, Activin RIIA, GFR alpha-3, Activin RIIB, GFR alpha-4, ALK-1, MIS R1I, ALK-7, Ret, BMPR-IA/ALK-3, TGF- beta RI/ALK-5, BMPR-IB/ALK-6, TGF-beta RII, BMPR-II, TGF-beta RIb, Endoglin/CD105 and TGF-beta RIII.

Yet other embodiments of the method comprise a Wnt-related molecule selected from the group consisting of Frizzled-1, Frizzled-8, Frizzled-2, Frizzled-9, Frizzled-3, sFRP-1, Frizzled-4, sFRP-2, Frizzled-5, sFRP-3, Frizzled-6, sFRP-4, Frizzled-7, MFRP, LRP 5, LRP 6, Wnt-1, Wnt-8a, Wnt-3a, Wnt-1Ob, Wnt-4, Wnt-11, Wnt-5a, Wnt-9a and Wnt-7a.

In other embodiments of the method, the receptor ligand is selected from the group consisting of 4-1BB Ligand/TNFSF9, Lymphotoxin, APRIL/TNFSF13, Lymphotoxin beta/TNFSF3, BAFF/TNFSF13C, OX40 Ligand/TNFSF4, CD27 Ligand/TNFSF7, TL1A/TNFSF15, CD30 Ligand/TNFSF8, TNF-alpha/TNFSF1A, CD40 Ligand/TNFSF5, TNF-beta/TNFSF1B, EDA-A2, TRAIL/TNFSF10, Fas Ligand/TNFSF6, TRANCE/TNFSF11, GITR Ligand/TNFSF18, TWEAK/TNFSF12, LIGHT/TNFSF14, Amphiregulin, NRG1 isoform GGF2, Betacellulin, NRG1 Isoform SMDF, EGF, NRGI-alpha/HRGl-alpha, Epigen, NRGT-beta.1/HRG1-beta 1, Epiregulin, TGF-alpha, HB-EGF, TMEFF1/Tomoregulin-1, Neuregulin-3, TMEFF2, IGF-I, IGF-I, Insulin, Activin A, Activin B, Activin AB, Activin C, BMP-2, BMP-7, BMP-3, BMP-8, BMP-3b/GDF-10, BMP-9, BMP-4, BMP-15, BMP-5, Decapentaplegic, BMP-6, GDF-1, GDF-8, GDF-3, GDF-9, GDF-5, GDF-11, GDF-6, GDF-15, GDF-7, Artemin, Neurturin, GDNF, Persephin, TGF-beta, TGF-beta 2, TGF-beta 1, TGF-beta 3, LAP (TGF-beta 1), TGF-beta 5, Latent TGF-beta 1, Latent TGF-beta bp1, TGF-beta 1.2, Lefty, Nodal, MIS/AMH, FGF acidic, FGF-12, FGF basic, FGF-13, FGF-3, FGF-16, FGF-4, FGF-17, FGF-5, FGF-19, FGF-6, FGF-20, FGF-8, FGF-21, FGF-9, FGF-23, FGF-10, KGF/FGF-7, FGF-11, Neuropilin-1, PGF, Neuropilin-2, PlGF-2, PDGF, PDGF-A, VEGF, PDGF-B, VEGF-B, PDGF-C, VEGF C, PDGF-D, VEGF-D and PDGF-AB.

In still other embodiments, the T-cell target is selected from the group consisting of 2B4/SLAMF4, IL-2 R alpha, 4-BB/TNFRSF9, IL-2 R beta, ALCAM, B7-1/CD80, IL-4 R, B7-H3, BLAME/SLAMF8, BTLA, IL-6 R, CCR3, IL-7 R alpha, CCR4, CXCR1I/IL-8 RA, CCR5, CCR6, IL-10 R alpha, CCR7, IL-10 R beta, CCR8, IL-12 R beta 1, CCR9, IL-12 R beta 2, CD2, IL-13 R alpha 1, IL-13, CD3, CD4, ILT2/CD85j, ILT3/CD85k, ILT4/CD85d, ILT5/CD85a, Integrin alpha 4/CD49d,

CD5, Integrin alpha E/CD103, CD6, Integrin alpha M/CDi b, CD8, Integrin alpha X/CD1Ic, Integrin beta 2/CD18, KIR/CDI58, CD27/TNFRSF7, KIR2DL1, CD28, KIR2DL3, CD30/TNFRSF8, KIR2DL4/CD158d, CD31/PECAM-1, KIR2DS4, CD40 Ligand/TNFSF5, LAG-3, CD43, LAIRI, CD45, LAIR2, CD83, Leukotriene B4 RI, CD84/SLAMF5, NCAM-L1, CD94, NKG2A, CD97, NKG2C, CD229/SLAMF3, NKG2D, CD2F-l0/SLAMF9, NT-4, CD69, NTB-A/SLAMF6, Common gamma Chain/IL-2 R gamma, Osteopontin, CRACC/SLAMF7, PD-1, CRTAM, PSGL-1, CTLA-4, RANK/TNFRSF1A, CX3CR1, CX3CL1, L-Selectin, CXCR3, SIRP beta 1, CXCR4, SLAM, CXCR6, TCCR/WSX-1, DNAM-1, Thymopoietin, EMMPRIN/CD147, TIM-1, EphB6, TIM-2, Fas/TNFRSF6, TIM-3, Fas Ligand/TNFSF6, TIM-4, Fc gamma RIII/CD16, TIM-6, GITR/TNFRSF18, TNF RI/TNFRSFlA, Granulysin, TNF RII/TNFRSFB, HVEM/TNFRSF14, TRAIL Ri/TNFRSF10A, ICAM-1/CD54, TRAIL R2/TNFRSFlOB, ICAM-2/CD102, TRAIL R3/TNFRSF1OC, IFN-gamma R1, TRAIL R4/TNFRSFOD, IFN-gamma R2, TSLP, IL-I RI and TSLP R.

In other embodiments, the NK cell receptor is selected from the group consisting of 2B4/SLAMF4, KIR2DS4, CD155/PVR, KIR3DLi, CD94, LMIR1/CD300A, CD69, LMIR2/CD300c, CRACC/SLAMF7, LMIR3/CD300LF, DNAM-1, LMIR5/CD300LB, Fc epsilon RII, LMIR6/CD300LE, Fc gamma RI/CD64, MICA, Fc gamma RIIB/CD32b, MICB, Fc gamma RIIC/CD32c, MULT-1, Fc gamma RIIA/CD32a, Nectin-2/CD112, Fe gamma RIII/CD16, NKG2A, FcRH/IRTA5, NKG2C, FcRH2/IRTA4, NKG2D, FcRH4/IRTAl, NKp30, FcRH5/IRTA2, NKp44, Fc Receptor-like 3/CD16-2, NKp46/NCR1, NKp8O/KLRF1, NTB-A/SLAMF6, Rae-1, Rae-1 alpha, Rae-I beta, Rae-i delta, H60, Rae-i epsilon, ILT2/CD85j, Rae-1 gamma, ILT3/CD85k, TREM-1, ILT4/CD85d, TREM-2, ILT5/CD85a, TREM-3, KIR/CD158, TREMLi/TLT-1, KIR2DL1, ULBP-1, KIR2DL3, ULBP-2, KIR2DL4/CD158d and ULBP-3.

In other embodiments, the monocyte/macrophage cell target is selected from the group consisting of B7-1/CD80, ILT4/CD85d, B7-Hi, ILT5/CD85a, Common beta Chain, Integrin alpha 4/CD49d, BLAME/SLAMF8, Integrin alpha X/CDIe, CCL6/C10, Integrin beta 2/CD18, CD155/PVR, Integrin beta 3/CD61, CD3i/PECAM-1, Latexin, CD36/SR-B3, Leukotriene B4 R, CD40/TNFRSF5,

LIMPII/SR-B2, CD43, LMIR1/CD300A, CD45, LMIR2/CD300c, CD68, LMIR3/CD300LF, CD84/SLAMF5, LMIR5/CD300LB, CD97, LMIR6/CD300LE, CD163, LRP-1, CD2F-10/SLAMF9, MARCO, CRACC/SLAMF7, MD-i, ECF-L, MD-2, EMMPRIN/CD147, MGL2, Endoglin/CD105, Osteoactivin/GPNMB, Fe gamma RI/CD64, Osteopontin, Fe gamma RIIB/CD32b, PD-L2, Fe gamma RIIC/CD32c, Siglec-3/CD33, Fc gamma RIIA/CD32a, SIGNR1/CD209, Fc gamma RIII/CD16, SLAM, GM-CSF R alpha, TCCR/WSX-1, ICAM-2/CD102, TLR3, IFN gamma Ri, TLR4, IFN-gamma R2, TREM-1, IL-i RII, TREM-2, ILT2/CD85j, TREM-3, ILT3/CD85k, TREML1/TLT-1, 2B4/SLAMF4, IL-10 R alpha, ALCAM, IL-10 R beta, Aminopeptidase N/ANPEP, ILT2/CD85j, Common beta Chain, ILT3/CD85k, Clq R1/CD93, ILT4/CD85d, CCR1, ILT5/CD85a, CCR2, Integrin alpha 4/CD49d, CCR5, Integrin alpha M/CDIIb, CCR8, Integrin alpha X/CDIIc, CD155/PVR, Integrin beta 2/CD18, CD14, Integrin beta 3/CD61, CD36/SR-B3, LAIRI, CD43, LAIR2, CD45, Leukotriene B4 R1, CD68, LIMPII/SR-B2, CD84/SLAMF5, LMIR1/CD300A, CD97, LMIR2/CD300c, CD163, LMIR3/CD300LF, Coagulation Factor III/Tissue Factor, LMIR5/CD300LB, CX3CR1, CX3CLi, LMIR6/CD300LE, CXCR4, LRP-1, CXCR6, M-CSF R, DEP 1/CD148, MD-I, DNAM-1, MD-2, EMMPRIN/CD147, MMR, Endoglin/CD105, NCAM-Li, Fe gamma RI/CD64, PSGL-1, Fc gamma RIII/CD16, RP105, G-CSF R, L-Selectin, GM-CSF R alpha, Siglec-3/CD33, HVEM/TNFRSF14, SLAM, ICAM 1/CD54, TCCR/WSX-1, ICAM-2/CD102, TREM-1, IL-6 R, TREM-2, CXCRI/IL-8 RA, TREM-3 and TREMLI/TLT-1.

In yet other embodiments of the method, a Dendritic cell target is selected from the group consisting of CD36/SR-B3, LOX-i/SR-El, CD68, MARCO, CD163, SR-AI/MSR, CD5L, SREC-I, CL-Pl/COLEC2, SREC-I, LIMPIISR-B2, RP105, TLR4, TLR1, TLR5, TLR2, TLR6, TLR3, TLR9,4-IBB Ligand/TNFSF9, IL-12/IL 23 p40,4-Amino-I,8-naphthalimide, ILT2/CD85j, CCL21/6Ckine, ILT3/CD85k, 8 oxo-dG, ILT4/CD85d, 8D6A, ILT5/CD85a, A2B5, Integrin alpha 4/CD49d, Aag, Integrin beta 2/CD18, AMICA, Langerin, B7-2/CD86, Leukotriene B4 R1, 7-13,

LMIR1/CD300A, BLAME/SLAMF8, LMIR2/CD300c, Clq RI/CD93, LMIR3/CD300LF, CCR6, LMIR5/CD300LB, CCR7, LMIR6/CD300LE, CD40/TNFRSF5, MAG/Siglec-4a, CD43, MCAM, CD45, MD-1, CD68, MD-2, CD83, MDL-i/CLEC5A, CD84/SLAMF5, MMR, CD97, NCAM-Li, CD2F-

10/SLAMF9, Osteoactivin/GPNMB, Chem 23, PD-L2, CLEC-1, RP105, CLEC-2, Siglec-2/CD22, CRACC/SLAMF7, Siglec-3/CD33, DC-SIGN, Siglec-5, DC SIGNR/CD299, Siglec-6, DCAR, Siglec-7, DCIR/CLEC4A, Siglec-9, DEC-205, Siglec-10, Dectin-1/CLEC7A, Siglec-F, Dectin-2/CLEC6A, SIGNRI/CD209, DEP 1/CD148, SIGNR4, DLEC, SLAM, EMMPRIN/CD147, TCCR/WSX-1, Fc gamma RI/CD64, TLR3, Fc gamma RIIB/CD32b, TREM-1, Fe gamma RIIC/CD32c, TREM 2, Fe gamma RIIA/CD32a, TREM-3, Fc gamma RIII/CD16, TREML1/TLT-1, ICAM-2/CD102 and Vanilloid RI.

In still other embodiments of the method, the angiogenesis target is selected from the group consisting of Angiopoietin-1, Angiopoietin-like 2, Angiopoietin-2, Angiopoietin-like 3, Angiopoietin-3, Angiopoietin-like 7/CDT6, Angiopoietin-4, Tie 1, Angiopoietin-like 1, Tie-2, Angiogenin, iNOS, Coagulation Factor III/Tissue Factor, nNOS, CTGF/CCN2, NOV/CCN3, DANCE, OSM, EDG-1, Plfr, EG VEGF/PK1, Proliferin, Endostatin, ROB04, Erythropoietin, Thrombospondin-1, Kininostatin, Thrombospondin-2, MFG-E8, Thrombospondin-4, Nitric Oxide, VG5Q, eNOS, EphAI, EphA5, EphA2, EphA6, EphA3, EphA7, EphA4, EphA8, EphBI, EphB4, EphB2, EphB6, EphB3, Ephrin-A1, Ephrin-A4, Ephrin-A2, Ephrin-A5, Ephrin-A3, Ephrin-B1, Ephrin-B3, Ephrin-B2, FGF acidic, FGF-12, FGF basic, FGF 13, FGF-3, FGF-16, FGF-4, FGF-17, FGF-5, FGF-19, FGF-6, FGF-20, FGF-8, FGF 21, FGF-9, FGF-23, FGF-10, KGF/FGF-7, FGF-11, FGF R1, FGF R4, FGF R2, FGF R5, FGF R3, Neuropilin-1, Neuropilin-2, Semaphorin 3A, Semaphorin 68, Semaphorin 3C, Semaphorin 6C, Semaphorin 3E, Semaphorin 6D, Semaphorin 6A, Semaphorin 7A, MMP, MMP-I 1, MMP-1, MMP-12, MMP-2, MMP-13, MMP-3, MMP-14, MMP-7, MMP-15, MMP-8, MMP-16/MT3-MMP, MMP-9, MMP 24/MT5-MMP, MMP-10, MMP-25/MT6-MMP, TIMP-1, TIMP-3, TIMP-2, TIMP-4, ACE, IL-13 R alpha 1, IL-13, Clq R1/CD93, Integrin alpha 4/CD49d, VE-Cadherin, Integrin beta 2/CD18, CD31/PECAM-1, KLF4, CD36/SR-B3, LYVE-1, CD151, MCAM, CL-P1/COLEC12, Nectin-2/CD112, Coagulation Factor III/Tissue Factor, E-Selectin, D6, P-Selectin, DC-SIGNR/CD299, SLAM, EMMPRIN/CD147, Tie-2, Endoglin/CD105, TNF RI/TNFRSFIA, EPCR, TNF RII/TNFRSF1B, Erythropoietin R, TRAIL R1/TNFRSF1OA, ESAM, TRAIL R2/TNFRSF1OB, FABP5, VCAM-1, ICAM-l/CD54, VEGF R2/Flk-1, ICAM-2/CD102, VEGF R3/Flt-4, IL-1 RI and VG5Q.

Other embodiments of the method provide multivalent binding proteins wherein at least one of binding domain I and binding domain 2 specifically binds a target selected from the group consisting of Prostate-specific Membrane Antigen (Folate Hydrolase 1), Epidermal Growth Factor Receptor (EGFR), Receptor for Advanced Glycation End products (RAGE, also known as Advanced Glycosylation End product Receptor or AGER), IL-17 A, IL-17 F, P19 (IL23A and IL12B), Dickkopf-l (Dkk1), NOTCH1, NG2 (Chondroitin Sulfate ProteoGlycan 4 or CSPG4), IgE (IgHE or IgH2), IL-22R (IL22RA1), IL-21, Amyloid P oligomers (Ab oligomers), Amyloid P Precursor Protein (APP), NOGO Receptor (RTN4R), Low Density LipoproteinReceptor-Related Protein 5 (LRP5), IL-4, Myostatin (GDF8), Very Late Antigen 4, an alpha 4, beta I integrin (VLA4 or ITGA4), an alpha 4, beta 7 integrin found on leukocytes, and IGF-1R. For example, aVLA4 target maybe recognized by a multivalent binding protein in which at least one of binding domain 1 and binding domain 2 is a binding domain derived from Natalizumab (Antegren).

In some embodiments, the cancer cell is a transformed, or cancerous, hematopoietic cell. In certain of these embodiments, at least one of the first binding domain and the second binding domain recognizes a target selected from the group consisting of a B-cell target, a monocyte/macrophage target, a dendritic cell target, an NK-cell target and a T-cell target, each as herein defined. Further, at least one of the first binding domain and the second binding domain can recognize a myeloid targets, including but not limited to, CD5, CD10, CD1ib, CD1Ic, CD13, CD14, CD15, CD18, CD19, CD20, CD21, CD22, CD23, CD25, CD27, CD29, CD30, CD31, CD33, CD34, CD35, CD38, CD43, CD45, CD64, CD66, CD68, CD70, CD80, CD86, CD87, CD88, CD89, CD98, CD100, CD103, CD111, CD112, CD114, CDl15, CD116, CD1 17, CD118, CD119, CD120a, CD120b, CDwl23, CDwl31, CD141, CD162, CD163, CD177, CD312, IRTA, IRTA2, IRTA3, IRTA4, IRTA5, B-B2, B-B8 and B cell antigen receptor.

Other embodiments of the invention are drawn to the multivalent binding protein, as described herein, comprising a sequence selected from the group consisting of SEQ ID NOS:2, 4, 6, 103, 105, 107, 109, 332, 333, 334, and 345. Other embodiments are directed to the multivalent binding protein comprising a sequence selected from the group consisting of SEQ IDNOS:355, 356, 357, 358, 359, 360, 361, 362, 363, 364 and 365.

In other embodiments, the multivalent and multispecific binding protein with effector function has a first binding domain and a second binding domain that recognize a target pair selected from the group consisting of EPHB4-KDR and TIE TEK. In such embodiments, the protein has a first binding domain recognizing EPHB4 and a second binding domain recognizing KDR or a first binding domain recognizing KDR and a second binding domain recognizing EPHB4. Analogously, the protein may have a first binding domain recognizing TIE and a second binding domain recognizing TEK, or a first binding domain recognizing TEK and a second binding domain recognizing TIE.

In a related aspect, the invention provides a multivalent binding protein with effector function, wherein the constant sub-region recognizes an effector cell Fc receptor (e.g., FcRI, FcyRII, FcyRIII, FcaR, and FcRI. In particular embodiments, the constant sub-region recognizes an effector cell surface protein selected from the group consisting of CD2, CD3, CD16, CD28, CD32, CD40, CD56, CD64, CD89, FctRI, KIR, thrombospondin R, NKG2D, 2B4/NAIL and 41BB. Theconstantsub region may comprise a CH2 domain and a CH3 domain derived from the same, or different, immunoglobuins, antibody isotypes, or allelic variants. In some embodiments, the CH3 domain is truncated and comprises a C-terminal sequence selected from the group consisting of SEQ ID NOS: 366, 367, 368, 369, 370 and 371. Preferably, the CH2 domain and the scorpion linker are derived from the same class, or from the same sub-class, of immunoglobulin, when the linker is a hinge-like peptide derived from an immunoglobulin.

Some proteins according to the invention are also contemplated as further comprising a scorpion linker of at least about 5 amino acids attached to the constant sub-region and attached to the second binding domain, thereby localizing the scorpion linker between the constant sub-region and the second binding domain. Typically, the scorpion linker peptide length is between 5-45 amino acids. Scorpion linkers include hinge-like peptides derived from immunoglobulin hinge regions, such as IgG1, IgG2, IgG3, IgG4, IgA, and IgE hinge regions. Preferably, a hinge-like scorpion linker will retain at least one cysteine capable of forming an interchain disulfide bond under physiological conditions. Scorpion linkers derived from IgG1 may have 1 cysteine or two cysteines, and will preferably retain the cysteine corresponding to an N-terminal hinge cysteine of IgG1. In some embodiments, the scorpion linker is extended relative to a cognate immunoglobulin hinge region and, in exemplary embodiments, comprises a sequence selected from the group consisting of SEQ ID NOS:351, 352, 353 and 354. Non-hinge-like peptides are also contemplated as scorpion linkers, provided that such peptides provide sufficient spacing and flexibility to provide a single-chain protein capable of forming two binding domains, one located towards each protein terminus (N and C) relative to a more centrally located constant sub region domain. Exemplary non-hinge-like scorpion linkers include peptides from the stalk region of type II C-lectins, such as the stalk regions of CD69, CD72, CD94, NKG2A and NKG2D. In some embodiments, the scorpion linker comprises a sequence selected from the group consisting of SEQ ID NOS:373, 374, 375, 376 and 377.

The proteins may also comprise a linker of at least about 5 amino acids attached to the constant sub-region and attached to the first binding domain, thereby localizing the linker between the constant sub-region and the first binding domain. In some embodiments, linkers are found between the constant sub-region and each of the two binding domains, and those linkers may be of the same or different sequence, and of the same or different lengths.

The constant sub-region of the proteins according to the invention provides at least one effector function. Any effector function known in the art to be associated with an immunoglobulin (e.g., an antibody) is contemplated, such as an effector function selected from the group consisting of antibody-dependent cell-mediated cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), relatively extended in vivo half-life (relative to the same molecule lacking a constant sub-region), FcR binding, protein A binding, and the like. In some embodiments, the extended half lives of proteins of the invention are at least 28 hours in a human. Of course, proteins intended for administration to non-human subjects will exhibit relatively extended half-lives in those non-human subjects, and not necessarily in humans.

In general, the proteins (including polypeptides and peptides) of the invention exhibit a binding affinity of less than 10 M, or at least 10-6 M, for at least one of the first binding domain and the second binding domain.

Another aspect of the invention is drawn to a pharmaceutical composition comprising a protein as described herein and a pharmaceutically acceptable adjuvant, carrier or excipient. Any adjuvant, carrier, or excipient known in the art is useful in the pharmaceutical compositions of the invention.

Yet another aspect of the invention provides a method of producing a protein as described above comprising introducing a nucleic acid encoding the protein into a host cell and incubating the host cell under conditions suitable for expression of the protein, thereby expressing the protein, preferably at a level of at least 1 mg/liter. In some embodiments, the method further comprises isolating the protein by separating it from at least one protein with which it is associated upon intracellular expression. Suitable host cells for expressing the nucleic acids to produce the proteins of the invention include, but are not limited to, a host cell selected from the group consisting of a VERO cell, a HeLa cell, a CHO cell, a COS cell, a W138 cell, a BHK cell, a HepG2 cell, a 3T3 cell, a RIN cell, an MDCK cell, an A549 cell, a PC12 cell, a K562 cell, a HEK293 cell, an N cell, a Spodopterafrugiperdacell, a Saccharomyces cerevisiae cell, a Pichiapastoris cell, any of a variety of fungal cells and any of a variety of bacterial cells (including, but not limited to, Escherichiacoli, Bacillus subtilis, Salmonella typhimurium, and a Streptomycete).

The invention also provides a method of producing a nucleic acid encoding the protein, as described above, comprising covalently linking the 3' end of a polynucleotide encoding a first binding domain derived from an immunoglobulin variable region to the 5' end of a polynucleotide encoding a constant sub-region ,

covalently linking the 5' end of a polynucleotide encoding a scorpion linker to the 3' end of the polynucleotide encoding the constant sub-region, and covalently linking the 5' end of a polynucleotide encoding a second binding domain derived from an immunoglobulin variable region to the 3' end of the polynucleotide encoding the scorpion linker, thereby generating a nucleic acid encoding a multivalent binding protein with effector function. Each of these coding regions may be separated by a coding region for a linker or hinge-like peptide as part of a single-chain structure according to the invention. In some embodiments, the method produces a polynucleotide encoding a first binding domain that comprises a sequence selected from the group consisting of SEQ ID NO: 2 (anti-CD20 variable region, oriented VL VH), SEQ ID NO: 4 (anti-CD28 variable region, oriented VL-VH) and SEQ ID NO: 6 (anti-CD28 variable region, oriented VH-V) in single-chain form, rather than requiring assembly from separately encoded polypeptides as must occur for heteromultimeric proteins, including natural antibodies. Exemplary polynucleotide sequences encoding first binding domains are polynucleotides comprising any of SEQ ID NOS: 1, 3 or 5.

This aspect of the invention also provides methods for producing encoding nucleic acids that further comprise a linker polynucleotide inserted between the polynucleotide encoding a first binding domain and the polynucleotide encoding a constant sub-region, the linker polynucleotide encoding a peptide linker of at least 5 amino acids. Additionally, these methods produce nucleic acids that further comprise a linker polynucleotide inserted between the polynucleotide encoding a constant sub region and the polynucleotide encoding a second binding domain, the linker polynucleotide encoding a peptide linker of at least 5 amino acids. Preferably, the encoded peptide linkers are between 5 and 45 amino acids.

The identity of the linker regions present either between BD1 and EFD or EFD and BD2 may be derived from other sequences identified from homologous -Ig superfamily members. In developing novel linkers derived from existing sequences present in homologous members of the -Ig superfamily, it may be preferable to avoid sequence stretches similar to those located between the end of a C-like domain and the transmembrane domain, since such sequences are often substrates for protease cleavage of surface receptors from the cell to create soluble forms. Sequence comparisons between different members of the -Ig superfamily and subfamilies can be compared for similarities between molecules in the linker sequences that join multiple V-like domains or between the V and C like domains. From this analysis, conserved, naturally occurring sequence patterns may emerge; these sequences when used as the linkers between subdomains of the multivalent fusion proteins should be more protease resistant, might facilitate proper folding between Ig loop regions, and would not be immunogenic since they occur in the extracellular domains of endogenous cell surface molecules.

The nucleic acids themselves comprise another aspect of the invention. Contemplated are nucleic acids encoding any of the proteins of the invention described herein. As such, the nucleic acids of the invention comprise, in 5' to 3' order, a coding region for a first binding domain, a constant sub-region sequence, and a coding region for a second binding domain. Also contemplated are nucleic acids that encode protein variants wherein the two binding domains and the constant sub region sequences are collectively at least 80%, and preferably at least 85%, 90%, 95%, or 99% identical in amino acid sequence to the combined sequences of a known immunoglobulin variable region sequence and a known constant sub-region sequence. Alternatively, the protein variants of the invention are encoded by nucleic acids that hybridize to a nucleic acid encoding a non-variant protein of the invention under stringent hybridization conditions of 0.015 M sodium chloride, 0.0015 M sodium citrate at 65-68°C or 0.015 M sodium chloride, 0.0015 M sodium citrate, and 50% formamide at 42°C. Variant nucleic acids of the invention exhibit the capacity to hybridize under the conditions defined immediately above, or exhibit 90%, 95%, 99%, or 99.9% sequence identity to a nucleic acid encoding a non-variant protein according to the invention.

In related aspects, the invention provides a vector comprising a nucleic acid as described above, as well as host cells comprising a vector or a nucleic acid as described herein. Any vector known in the art may be used (e.g., plasmids, phagemids, phasmids, cosmids, viruses, artificial chromosomes, shuttle vectors and the like) and those of skill in the art will recognize which vectors are particularly suited for a given purpose. For example, in methods of producing a protein according to the invention, an expression vector operable in the host cell of choice is selected. In like manner, any host cell capable of being genetically transformed with a nucleic acid or vector of the invention is contemplated. Preferred host cells are higher eukaryotic host cells, although lower eukaryotic (e.g., yeast) and prokaryotic (bacterial) host cells are contemplated.

Another aspect of the invention is drawn to a method of inducing damage to a target cell comprising contacting a target cell with a therapeutically effective amount of a protein as described herein. In some embodiments, the target cell is contacted in vivo by administration of the protein, or an encoding nucleic acid, to an organism in need. Contemplated within this aspect of the invention are methods wherein the multivalent single-chain binding protein induces an additive amount of damage to the target cell, which is that amount of damage expected from the sum of the damage attributable to separate antibodies comprising one or the other of the binding domains. Also contemplated are methods wherein the multivalent single-chain binding protein induces a synergistic amount of damage to the target cell compared to the sum of the damage induced by a first antibody comprising the first binding domain but not the second binding domain and a second antibody comprising the second binding domain but not the first binding domain. In some embodiments, the multivalent single-chain binding protein is multispecific and comprises a binding domain pair specifically recognizing a pair of antigens selected from the group consisting of CD19/CD20, CD20/CD21, CD20/CD22, CD20/CD40, CD20/CD79a, CD20/CD79b, CD20/CD81, CD21/CD79b, CD37/CD79b, CD79b/CD81, CD19/CL II (i.e., MHC class II), CD20/CL II, CD30/CL II, CD37/CL II, CD72/CL II, and CD79b/CL I.

This aspect of the invention also comprehends methods wherein the multispecific, multivalent single-chain binding protein induces an inhibited amount of damage to the target cell compared to the sum of the damage induced by a first antibody comprising the first binding domain but not the second binding domain and a second antibody comprising the second binding domain but not the first binding domain. Exemplary embodiments include methods wherein the multi-specific, multivalent single-chain binding protein comprises a binding domain pair specifically recognizing a pair of antigens selected from the group consisting of CD20/CL II, CD21/CD79b, CD22/CD79b, CD40/CD79b, CD70/CD79b, CD72/CD79b, CD79a/CD79b, CD79b/CD80, CD79b/CD86, CD21/CL II, CD22/CL II, CD23/CL II, CD40/CL II, CD70/CL II, CD80/CL II, CD86/CL II, CD19/CD22, CD20/CD22, CD21/CD22, CD22/CD23, CD22/CD30, CD22/CD37, CD22/CD40, CD22/CD70, CD22/CD72, CD22/79a, CD22/79b, CD22/CD80, CD22/CD86 and CD22/CL II.

In a related aspect, the invention provides a method of treating a cell proliferation disorder, e.g., cancer, comprising administering a therapeutically effective amount of a protein (as described herein), or an encoding nucleic acid, to an organism in need. Those of skill in the art, including medical and veterinary professionals, are proficient at identifying organisms in need of treatment. Disorders contemplated by the invention as amenable to treatment include a disorder selected from the group consisting of a cancer, an autoimmune disorder, Rous Sarcoma Virus infection and inflammation. In some embodiments, the protein is administered by in vivo expression of a nucleic acid encoding the protein as described herein. The invention also comprehends administering the protein by a route selected from the group consisting of intravenous injection, intraarterial injection, intramuscular injection, subcutaneous injection, intraperitoneal injection and direct tissue injection.

Another aspect of the invention is directed to a method of ameliorating a symptom associated with a cell proliferation disorder comprising administering a therapeutically effective amount of a protein, as described herein, to an organism in need. Those of skill in the art are also proficient at identifying those disorders, or diseases or conditions, exhibiting symptoms amenable to amelioration. In some embodiments, the symptom is selected from the group consisting of pain, heat, swelling and joint stiffness.

Yet another aspect of the invention is drawn to a method of treating an infection associated with an infectious agent comprising administering a therapeutically effective amount of a protein according to the invention to a patient in need, wherein the protein comprises a binding domain that specifically binds a target molecule of the infectious agent. Infectious agents amenable to treatment according to this aspect of the invention include prokaryotic and eukaryotic cells, viruses (including bacteriophage), foreign objects, and infectious organisms such as parasites (e.g., mammalian parasites).

A related aspect of the invention is directed to a method of ameliorating a symptom of an infection associated with an infectious agent comprising administering an effective amount of a protein according to the invention to a patient in need, wherein the protein comprises a binding domain that specifically binds a target molecule of the infectious agent. Those of skill in the medical and veterinary arts will be able to determine an effective amount of a protein on a case-by-case basis, using routine experimentation,

Yet another related aspect of the invention is a method of reducing the risk of infection attributable to an infectious agent comprising administering a prophylactically effective amount of a protein according to the invention to a patient at risk of developing the infection, wherein the protein comprises a binding domain that specifically binds a target molecule of the infectious agent. Those of skill in the relevant arts will be able to determine a prophylactically effective amount of a protein on a case-by-case basis, using routine experimentation.

Another aspect of the invention is drawn to the above-described multivalent single-chain binding protein wherein at least one of the first binding domain and the second binding domain specifically binds an antigen selected from the group consisting of CD19, CD20, CD21, CD22, CD23, CD30, CD37, CD40, CD70, CD72, CD79a, CD79b, CD80, CD81, CD86, and a major histocompatibility complex class II peptide.

In certain embodiments, one of the first binding domain and the second binding domain specifically binds CD20, and in some of these embodiments, the other binding domain specifically binds an antigen selected from the group consisting of CD19, CD20, CD21, CD22, CD23, CD30, CD37, CD40, CD70, CD72, CD79a, CD79b, CD80, CD81, CD86, and a major histocompatibility complex class II peptide. For example, in one embodiment, the first binding domain is capable of specifically binding CD20 while the second binding domain is capable of specifically binding, e.g.,CD19. In another embodiment, the first binding domain binds CD19 while the second binding domain binds CD20. An embodiment in which both binding domains bind CD20 is also contemplated.

In certain other embodiments according to this aspect of the invention, one of the first binding domain and the second binding domain specifically binds CD79b, and in some of these embodiments, the other binding domain specifically binds an antigen selected from the group consisting of CD19, CD20, CD21, CD22, CD23, CD30, CD37, CD40, CD70, CD72, CD79a, CD79b, CD80, CD81, CD86, and a major histocompatibility complex class II peptide. Exemplary embodiments include distinct multi-specific, multivalent single-chain binding proteins in which a first binding domain:second binding domain specifically binds CD79b:CD19 or CDI9:CD79b. A multivalent binding protein having first and second binding domains recognizing CD79b is also comprehended.

In still other certain embodiments, one of the first binding domain and the second binding domain specifically binds a major histocompatibility complex class II peptide, and in some of these embodiments, the other binding domain specifically binds an antigen selected from the group consisting of CD19, CD20, CD21, CD22, CD23, CD30, CD37, CD40, CD70, CD72, CD79a, CD79b, CD80, CD81, CD86, and a major histocompatibility complex class II peptide. For example, in one embodiment, the first binding domain is capable of specifically binding a major histocompatibility complex class II peptide while the second binding domain is capable of specifically binding, e.g., CD19. In another embodiment, the first binding domain binds CD19 while the second binding domain binds a major histocompatibility complex class II peptide. An embodiment in which both binding domains bind a major histocompatibility complex class II peptide is also contemplated.

In yet other embodiments according to this aspect of the invention, one of the first binding domain and the second binding domain specifically binds CD22, and in some of these embodiments, the other binding domain specifically binds an antigen selected from the group consisting of CD19, CD20, CD21, CD22, CD23, CD30, CD37, CD40, CD70, CD72, CD79a, CD79b, CD80, CD81, CD86, and a major histocompatibility complex class II peptide. Exemplary embodiments include distinct multi-specific, multivalent single-chain binding proteins in which a first binding domain:second binding domain specifically binds CD22:CD19 or CD19:CD22. A multivalent binding protein having first and second binding domains recognizing CD22 is also comprehended.

A related aspect of the invention is directed to the above-described multivalent single-chain binding protein wherein the protein has a synergistic effect on a target cell behavior relative to the sum of the effects of each of the binding domains. In some embodiments, the protein comprises a binding domain pair specifically recognizing a pair of antigens selected from the group consisting of CD20-CD19, CD20-CD21, CD20-CD22, CD20-CD40, CD20-CD79a, CD20-CD79b and CD20 CD81.

The invention further comprehends a multivalent single-chain binding protein as described above wherein the protein has an additive effect on a target cell behavior relative to the sum of the effects of each of the binding domains. Embodiments according to this aspect of the invention include multi-specific proteins comprising a binding domain pair specifically recognizing a pair of antigens selected from the group consisting of CD20-CD23, CD20-CD30, CD20-CD37, CD20-CD70, CD20 CD80, CD20-CD86, CD79b-CD37, CD79b-CD81, major histocompatibility complex class II peptide-CD30, and major histocompatibility complex class II peptide-CD72.

Yet another related aspect of the invention is a multivalent single-chain binding protein as described above wherein the protein has an inhibitory effect on a target cell behavior relative to the sum of the effects of each of the binding domains. In some embodiments, the protein is multispecific and comprises a binding domain pair specifically recognizing a pair of antigens selected from the group consisting of CD20-major histocompatibility complex class II peptide, CD79b-CD19, CD79b CD20, CD79b-CD21, CD79b-CD22, CD79b-CD23, CD79b-CD30, CD79b-CD40, CD79b-CD70, CD79b-CD72, CD79b-CD79a, CD79b-CD80, CD79b-CD86, CD79b major histocompatibility complex class II peptide, major histocompatibility complex class II peptide-CD19, major histocompatibility complex class II peptide-CD20, major histocompatibility complex class II peptide-CD21, major histocompatibility complex class II peptide-CD22, major histocompatibility complex class Il peptide CD23, major histocompatibility complex class II peptide-CD37, major histocompatibility complex class II peptide-CD40, major histocompatibility complex class II peptide-CD70, major histocompatibility complex class II peptide-CD79a, major histocompatibility complex class II peptide-CD79b, major histocompatibility complex class II peptide-CD80, major histocompatibility complex class II peptide CD81, major histocompatibility complex class II peptide-CD86, CD22-CD19, CD22 CD40, CD22-CD79b, CD22-CD86 and CD22-major histocompatibility complex class II peptide.

Another aspect of the invention is a method of identifying at least one of the binding domains of the multivalent binding molecule, such as a multispecific binding molecule, described above comprising: (a) contacting an anti-isotypic antibody with an antibody specifically recognizing a first antigen and an antibody specifically recognizing a second antigen; (b) further contacting a target comprising at least one of said antigens with the composition of step (a); and (c) measuring an activity of the target, wherein the activity is used to identify at least one of the binding domains of the multivalent binding molecule. In some embodiments, the target is a diseased cell, such as a cancer cell (e.g., a cancerous B-cell) or an auto-antibody-producing B-cell.

In each of the foregoing methods of the invention, it is contemplated that the method may further comprise a plurality of multivalent single-chain binding proteins. In some embodiments, a binding domain of a first multivalent single-chain binding protein and a binding domain of a second multivalent single-chain binding protein induce a synergistic, additive, or inhibitory effect on a target cell, such as a synergistic, additive, or inhibitory amount of damage to the target cell. The synergistic, additive or inhibitory effects of a plurality of multivalent single-chain binding proteins is determined by comparing the effect of such a plurality of proteins to the combined effect of an antibody comprising one of the binding domains and an antibody comprising the other binding domain.

A related aspect of the invention is directed to a composition comprising a plurality of multivalent single-chain binding proteins as described above. In some embodiments, the composition comprises a plurality of multivalent single-chain binding proteins wherein a binding domain of a first multivalent single-chain binding protein and a binding domain of a second multivalent single-chain binding protein are capable of inducing a synergistic, additive, or inhibitory effect on a target cell, such as a synergistic, additive or inhibitory amount of damage to the target cell.

The invention further extends to a pharmaceutical composition comprising the composition described above and a pharmaceutically acceptable carrier, diluent or excipient, In addition, the invention comprehends a kit comprising the composition as described herein and a set of instructions for administering said composition to exert an effect on a target cell, such as to damage the target cell.

Finally, the invention also comprehends a kit comprising the protein as described herein and a set of instructions for administering the protein to treat a cell proliferation disorder or to ameliorate a symptom of the cell proliferation disorder.

Other features and advantages of the present invention will be better understood by reference to the following detailed description, including the examples.

BRIEF DESCRIPTION OF THE DRAWING

Figure 1 shows a schematic representation of the multivalent single-chain molecules envisioned by the invention. Individual subdomains of the fusion protein expression cassette are indicated by separate shapes/blocks on the figure. BD1 refers to binding domain 1, linker 1 refers to any potential linker or hinge like peptide between BD Iand the "effector function domain", indicated as EFD. This subdomain is usually an engineered form of the Fe domain of human IgGI, but may include other subdomains with one or more effector functions as defined herein. Linker 2 refers to the linker sequence, if any, present between the carboxy terminus of the EFD and the binding domain 2, 802.

Figure 2 shows a Western blot of non-reduced proteins expressed in COS cells. Protein was secreted into the culture medium, and culture supernatants isolated after 48-72 hours from transiently transfected cells by centrifugation. Thirty microliters, 30 pl of crude supernatant were loaded into each well of the gel. Lane identifications: 1- molecular weight markers, with numerals indicating kilodaltons; 2 2H7-IgG-STD1-2E12 LH; 3- 2H7-IgG-STD1-2E12 HL, 4- 2H7-IgG-STD2-2E12 LH; 5- 2H7-IgG-STD2-2E12 HL; 6- 2E12 LH SMIP; 7- 2E12 HL SMIP; 8- 2H7 SMIP. "2H7" refers to a single-chain construct, where BD1 encodes the CD20 specific binding domain (2H7) in the VLVH orientation; "2E12" refers to a binding domain specific for CD28; -IgG-refers to a single-chain construct, with a hinge encoding a sequence where all C are mutated to S (sss), and the CH2 and CH3 domains of IgG1 contain mutations which eliminate both ADCC and CDC effector functions (P238S and P331 S), "STD 1 refers to a 20-amino-acid linker (identified in Figure 7 as "STD1=20aa ") inserted adjacent to the 8D2 in the VL-VH orientation, or 2E12 (VL VH). "STDI- HL" refers to a similar construct as just described, but with the BD2 V regions in the VH-VL orientation as follows: 2H7-sssIgG(P238/331S)-20-amino acid linker-2E12 (VH-VL). "STD2- LH" refers to 2H7-sssIgG (P238/331S)-38-amino acid linker-2E12 (VL-VI); "STD2-LH" refers to 2H7-sssIgG (P238/331SS)-38 amino-acid linker-2E12 (VH-VL); "SMIP" refers to small modular immunopharmaceutical; and "H" generally refers to VH, while "L" generally refers to

VL. Unless otherwise indicated, all protein orientations are N-terminal to C-terminal orientations.

Figure 3 shows two columnar graphs illustrating the binding properties of the 2H7-sssIgG (P238S/P331S)-STD-2e12 LH and HL derivatives expressed from COS cells. These experiments were performed with crude culture supernatants rather than purified proteins. Serial dilutions from undiluted to 16X of the culture supernatants were incubated with either CD20 expressing cells (WIL-2S) or CD28 expressing cells (CD28 CHO). Binding activity in the supernatants was compared to control samples testing binding of the relevant single specificity SMIP, such as TRU-015, or 2e12 VLVH, or 2e12VHVL SMIPs. Binding in each sample was detected using fluorescein isothyocyanate (FITC) conjugated goat anti-human IgG at a dilution of 1:100.

Figure 4 is a histogram showing the binding pattern of protein A purified versions of the proteins tested in Figure 3 to WIL2-S cells. "TRU015" is a SMIP specific for CD20. Two multispecific binding proteins with effector function were also analyzed: "2H7-2E12 LH" has binding domain 2, specific for CD28, in VL-VH orientation; "2H7-2E12 HL" has binding domain 2, specific for CD28, in VH-VL orientation. Each of the proteins was tested for binding at 5 Ig/ml, and binding detected with FITC goat anti-human IgG at 1:100. See the description for Figure 2 above for more complete descriptions of the molecules tested.

Figure 5 shows two histograms illustrating the binding by protein A purified multispecific binding proteins with effector function to CHO cells expressing CD28. "2H7-2E12 LH" has binding domain 2, specific for CD28, in VL-VH orientation; "2H7-2E12 HL" has binding domain 2, specific for CD28, in VH-VL orientation. Each of the proteins was tested for binding at 5 pg/ml, and binding was detected with FITC goat anti-humanIgGat 1:100. Seethe descriptions in Figure 2 for a more complete description of the molecules tested.

Figure 6 A) shows a table which identifies the linkers joining the constant sub-region and binding domain 2. The linkers are identified by name, sequence, sequence identifier, sequence length, and the sequence of the fusion with binding domain 2. B) shows a table identifying a variety of constructs identifying elements of exemplified molecules according to the invention.. In addition to identifying the multivalent binding molecules by name, the elements of those molecules are disclosed in terms of binding domain 1 (BD1), the constant sub-region (hinge and effector domain or EFD), a linker (see Fig. 6A for additional information regarding the linkers), and binding domain 2 (BD2). The sequences of a number of exemplified multivalent binding proteins are provided, and are identified in the figure by a sequence identifier. Other multivalent binding proteins have altered elements, or element orders, with predictable alterations in sequence from the disclosed sequences.

Figure 7 shows a composite columnar graph illustrating the binding of purified proteins at a single, fixed concentration to CD20 expressing WIL-2S cells and to CHO cells expressing CD28. "H1-H6" refers to the 2H7-sss-hgG-Hx-2e12 molecules with the H1-H6 linkers and the 2e12 V regions in the orientationof VH-VL. "L I -L6" refers to the 2H7-sss-hgG-Lx-2e12 molecules with the L1-L6 linkers and the 2e12 V regions in the orientation of VL-VH. All the molecules were tested at a concentration of 0.72 gg/ml, and the binding detected using FITC conjugated goat anti-human IgG at 1:100. The mean fluorescence intensity for each sample was then plotted as paired bar graphs for the two target cell types tested versus each of the multivalent constructs being tested, Ll-L6, or H1-H6.

Figure 8 shows photographs of Coomassie stained non-reducing and reducing SDS-PAGE gels. These gels show the effect of the variant linker sequence/length on the 2H7-sss-hlgG-Hx-2e12 HL protein on the amounts of the two predominate protein bands visualized on the gel.

Figure 9 shows Western Blots of the [2H7-sss-hIgG-H6-2e12] fusion proteins and the relevant single specificity SMIPs probed with either (a) CD28mIgG or with (b) a Fab reactive with the 2H7 specificity. The results show that the presence of the H6 linker results in the generation of cleaved forms of the multivalent constructs which arc missing the CD28 binding specificity.

Figure 10 shows binding curves of the different linker variants for the

[TRU015-sss-IgG-Hx-2e12 HL] H1-H6 linker forms. The first panel shows the binding curves for binding to CD20 expressing WIL-2S cells. The second panel shows the binding curves for binding of the different forms to CD28 CHO cells.

These binding curves were generated with serial dilutions of protein A purified fusion protein, and binding detected using FITC conjugated goat anti-human IgG at 1:100.

Figure 11 shows a table summarizing the results of SEC fractionation of 2H7 sss-IgG-2e12 HL multispecific fusion proteins with variant linkers HI-H7. Each row in the table lists a different linker variant of the [2H7-sss-IgG-Hx-2e2-HL] fusion proteins. The retention time of the peak of interest (POI), and the percentage of the fusion protein present in POI, and the percentage of protein found in other forms is also tabulated. The cleavage of the molecules is also listed, with the degree of cleavage indicated in a qualitative way, with (Yes), Yes, and YES, or No being the four possible choices.

Figure 12 shows two graphs with binding curves for [2H7-sss-hIgG-Hx-2e121 multispecific fusion proteins with variant linkers H3, H6, and H7 linkers to cells expressing CD20 or CD28. Serial dilutions of the protein A purified fusion proteins from 10 pg/ml down to 0.005 sg/ml were incubated with either CD20 expressing WIL-2S cells or CD28 CHO cells. Binding was detected using FITC conjugated goat anti-human IgG at 1:100. Panel A shows the binding to WIL-2S cells, and panel B shows the binding to CD28 CHO cells.

Figure 13 shows the results of an alternative binding assay generated by the molecules used for Figure 12. In this case, the fusion proteins were first bound to WIL-2S CD20 expressing cells, and binding was then detected with CD28mIgG (5 pig/ml)and FITC anti-mouse reagent at 1:100. These results demonstrate the simultaneous binding to both CD20 and CD28 in the same molecule.

Figure 14 shows results obtained using another multispecific fusion construct variant. In this case, modifications were made in the specificity for BD2, so that the V regions for the G28-1 antibody were used to create a CD37 specific binding domain. Shown are two graphs which illustrate the relative ability of CD20 and/or CD37 antibodies to block the binding of the[2H7-sss-IgG-Hx-G28-1] multispecific fusion protein to Ramos or BJAB cells expressing the CD20 and CD37 targets. Each cell type was preincubated with either the CD20 specific antibody (25 pg/ml) or the CD37 specific antibody (10 ptg/ml) or both reagents (these are mouse anti-human reagents) prior to incubation with the multispecific fusion protein. Binding of the multispecific fusion protein was then detected with a FITC goat anti-human IgG reagent at 1:100, (preadsorbed to mouse to eliminate cross-reactivity).

Figure 15 shows the results of an ADCC assay performed with BJAB target cells, PBMC effector cells, and with the CD20-hIgG-CD37 specific fusion protein as the test reagent. For a full description of the procedure see the appropriate example. The graph plots the concentration of fusion protein versus the % specific killing at each dosage tested for the single specificity SMIP reagents, and for the [2H7-sss hIgG-STDI-G28-1] LH and HL variants. Each data series plots the dose-response effects for one of these single specificity or multispecific single-chain fusion proteins.

Figure 16 shows a table tabulating the results of a co-culture experiment where PBMC were cultured in the presence of TRU 015, G28-1 SMIP, both molecules together, or the [2H7-sss-IgG-H7-G28-lHL] variant. The fusion proteins were used at 20 g/ml, and incubated for 24 hours or 72 hours. Samples were then stained with CD3 antibodies conjugated to FITC, and either CD19 or CD40 specific antibodies conjugated to PE, then subjected to flow cytometry. The percentage of cells in each gate was then tabulated.

Figure 17 shows two columnar graphs of the effects on B cell line apoptosis after 24 hour incubation with the [2H7-sss-hIgG-H7-G28-1 HL] molecule or control single CD20 and/or CD37 specificity SMIPs alone or in combination. The percentage of annexin V-propidium iodide positive cells is plotted as a function of the type of test reagent used for the coincubation experiments. Panel A shows the results obtained using Ramos cells, and panel B shows those for Daudi cells. Each single CD20 or CD37 directed SMIP is shown at the concentrations indicated; in addition, where combinations of the two reagents were used, the relative amount of each reagent is shown in parentheses. For the multispecific CD20-CD37 fusion protein, concentrations of 5, 10, and 20 pg/ml were tested.

Figure 18 shows two graphs of the [2H7-hgG-G19-4] molecule variants and their binding to either CD3 expressing cells (Jurkats) or CD20 expressing cells (WIL 2S). The molecules include [2H7-sss-hIgG-STD1-G19-4 HL], LH, and [2H7-csc hIgG-STD1-G19-4 HL]. Protein A purified fusion proteins were titrated from 20 gg/mi down to 0.05 pg/ml, and the binding detected using FITC goat anti-human IgG at 1:100. MFJ (mean fluorescence intensity) is plotted as a function of protein concentration.

Figure 19 shows the results of ADCC assays performed with the [2H7-hIgG STDI-G19-4 HL] molecule variants with either an SSS hinge or a CSC hinge, BJAB target cells, and either total human PBMC as effector cells or NK cell depleted PBMC as effector cells. Killing was scored as a function of concentration of the multispecific fusion proteins. The killing observed with these molecules was compared to that seen using G19-4, TRU 015, or a combination of these two reagents. Each data series plots a different test reagent, with the percent specific killing plotted as a function of protein concentration.

Figure 20 shows the percentage of Ramos B-cells that stained positive with Annexin V (Ann) and/or propidium iodide (PI) after overnight incubation with each member of a matrix panel of B-cell antibodies (2 pg/ml) in the presence, or absence, of an anti-CD20 antibody (present at 2 pg/ml where added). Goat-anti-mouse secondary antibody was always present at a two-fold concentration ratio relative to other antibodies (either matrix antibody alone, or matrix antibody and anti-CD20 antibody). Vertically striped bars - matrix antibody (2 g/ml) denoted on X-axis and goat anti-mouse antibody (4 jg/ml). Horizontally striped bars - matrix antibody (2

[tg/ml) denoted on X-axis, anti-CD20 antibody (2 pg/ml), and goat anti-mouse antibody (4 pg/ml). The "2" step" condition served as a control and involved the addition of goat anti-mouse antibody at 4 g/ml (vertically striped bar) or 8pg/ml (horizontally striped bar), without a matrix antibody or anti-CD20 antibody. "CL I" (MHC class 11) in the figures refers to a monoclonal antibody cross-reactive to HLA DR, DQ and DP, i.e., to MHC Class II antigens.

Figure 21 shows the percentage of Ramos B-cells that stained positive with Annexin V (Ann) and/or propidium iodide (PI) after overnight incubation with each member of a matrix panel of B-cell antibodies (2 g/ml) in the presence, or absence, of an anti-CD79b antibody (present at either 0.5 or 1.0 pg/ml where added). See the description of Figure 20 for identification of "CL II" and "2"step" samples. Vertically striped bars - matrix antibody (2 [g/ml) and goat anti-mouse antibody (4 jig/ml); horizontally striped bars - matrix antibody (2 jg/ml), anti-CD79b antibody

(1.0 pg/ml) and goat anti-mouse antibody (6 pg/ml); stippled bars - matrix antibody (2 g/ml), anti-CD79b antibody (0.5 g/ml) and goat anti-mouse antibody (5 pg/ml).

Figure 22 shows the percentage of Ramos B-cells that stained positive with Annexin V (Ann) and/or propidium iodide (PI) after overnight incubation with each member of a matrix panel of B-cell antibodies (2 pg/ml) in the presence, or absence, of an anti-CL II antibody (present at either 0.25 or 0.5 g/ml where added). See the description of Figure 20 for identification of "CLII" and "2" step" samples. Vertically striped bars - matrix antibody (2 pg/ml) and goat anti-mouse antibody (4 pg/ml); horizontally striped bars - matrix antibody (2 pg/ml), anti-CL II antibody (0.5 pg/ml) and goat anti-mouse antibody (5 pg/ml); stippled bars - matrix antibody (2 pg/ml), anti-CL II antibody (0.25 pg/ml) and goat anti-mouse antibody (4.5 g/ml).

Figure 23 shows the percentage of DHL-4 B-cells that stained positive with Annexin V (Ann) and/or propidium iodide (PI) after overnight incubation with each member of a matrix panel of B-cell antibodies (2 g/ml) in the presence, or absence, of an anti-CD22 antibody (present at 2 g/ml where added). See the description of Figure 20 for identification of "CL II" and "2"d step" samples. Solid bars - matrix antibody (2 pg/ml) and goat anti-mouse antibody (4 g/ml); slant-striped bars matrix antibody (2 pg/ml), anti-CD22 antibody (2 g/ml) and goat anti-mouse antibody (8 g/ml).

Figure 24 provides a graph demonstrating direct growth inhibition of lymphoma cell lines Su-DHL6 (triangles) and DoHH2 (squares) by free CD20 SMIP (closed symbols) or monospecific CD20xCD20 scorpion (open symbols).

Figure 25 is a graph showing direct growth inhibition of lymphoma cell lines Su-DHL-6 (triangles) and DoHH2 (squares) by free anti-CD37 SMIP (closed symbols) or monospecific anti-CD37 scorpion (open symbols).

Figure 26 presents a graph showing direct growth inhibition of lymphoma cell lines Su-DHL-6 (triangles) and DoHH2 (squares) by a combination of two different monospecific SMIPs (closed symbols) or by a bispecific CD20-CD37 scorpion (open symbols).

Figure 27 is a graph revealing direct growth inhibition of lymphoma cell lines Su-DHL-6 (triangles) and WSU-NHL (squares) by free CD20 SMIP and CD37 SMIPcombination (closed symbols) or bispecific CD20xCD37 scorpion (open symbols).

Figure 28 provides histograms showing the cell-cycle effects of scorpions. Samples of DoHH2 lymphoma cells were separately left untreated, treated with SMIP 016 or treated with the monospecific CD37 x CD37 scorpion. Open bars: sub-Gi phase of the cell cyle; black bars: Go/G 1 phase; shaded: S phase; and striped: G2/M phase.

Figure 29 presents graphs of data establishing that treatment of lymphoma cells with scorpions resulted in increased signaling capacity compared to free SMIPs, as measured by calcium ion flux.

Figure 30 provides graphs demonstrating scorpion-dependent cellular cytotoxicity

Figure 31 shows graphs of data indicating that scorpions mediate Complement Dependent Cytotoxicity.

Figure 32 provides data in graphical form showing comparative ELISA binding of a SMIP and a scorpion to low- (B) and high-affinity (A) isoforms of FcyRIII (CD16).

Figure 33 presents graphs establishing the binding of a SMIP and a scorpion to low (A)- and high (B)-affinity allelotypes of FcyRIII (CD16) in the presence of target cells.

Figure 34 is a histogram showing the expression level of a CD20 x CD20 scorpion in two experiments (flask 1 and flask 2) under six different culturing conditions. Solid black bars: flask 1; striped bars: flask 2.

Figure 35 provides a histogram showing the production yield of a CD20 x CD37 scorpion.

Figure 36 presents SDS-PAGE gels (under reducing and non-reducing conditions) of a SMIP and a scorpion.

Figure 37 provides a graph showing that scorpions retain the capacity to bind to target cells. Filled squares: CD20 SMIP; filled triangles: CD37 SMIP; filled circles: humanized CD20 (2Lm20-4) SMIP; open diamond: CD37 x CD37 monospecific scorpion; open squares: CD20 x CD37 bi-specific scorpion; and open triangles: humanized CD20 (2Lm2O-4) x humanized CD20 (2Lm2O-4) scorpion.

Figure 38 contains graphs showing the results of competitive binding assays establishing that both N- and C-terminal scorpion binding domains participate in target cell binding.

Figure 39 presents data in the form of graphs showing that scorpions have lower off-rates than SMIPs.

Figure 40 shows a graph establishing that scorpions are stable in serum in vivo, characterized by a reproducible,sustained circulating half-life for the scorpion.

Figure 41 provides a dose-response graph for a CD20 x CD37 bispecific scorpion, demonstrating the in vivo efficacy of scorpion administration.

Figure 42 shows target B-cell binding by a monospecific CD20xCD2O scorpion (SO129) and glycovariants.

Figure 43 provides graphs illustrating CD20xCD2O scorpions (parent and glycovariants) inducing ADCC-mediated killing of BJAB B-cells.

Figure 44 shows a gel revealing the effects on scorpion stability arising from changes in the scorpion linker, including changing the sequence of that linker and extending the linker by adding an H7 sequence to the linker, indicated by a "+" in the H7 line under the gel.

Figure 45 shows the binding to WIL2S cells of a CD20xCD20 scorpion (SO129) and scorpion linker variants thereof.

Figure 46 shows the direct cell killing of a variety of B-cells by a CD20xCD20 scorpion and by a CD20 SMIP.

Figure 47 reveals the direct cell killing of additional B-cell lines by a monospecific CD20xCD20 scorpion.

Figure 48 shows the direct cell killing capacities of each of two monospecific scorpions, i.e., CD20xCD20 and CD37xCD37, and a bispecific CD20xCD37 scorpion, the latter exhibiting a different form of kill curve.

Figure 49 graphically depicts the response of Su-DHL-6 B-cells to each of a CD20xCD20 (80129), a CD37xCD37, and a CD20xCD37 scorpion.

Figue 50 shows the capacity of a bispecific CD19xCD37 scorpion and Rituxan* to directly kill Su-DHL-6 B-cells.

Figure 51 provides histograms showing the direct killing of DHL-4 B-cells by a variety of CD20-binding scorpions and SMIPs, as well as by Rituxan®, as indicated in the figure. Blue bars: live cells; maroon bars on the right of each pair: Annexin+/PI+.

Figure 52 provides a graphic depiction of the direct cell killing of various CD20-binding scorpions and SMIPs, as well as by Rituxan*, as indicated in the figure.

Figure 53 provides graphs of the ADCC activity induced by various CD20 binding scorpions and SMIPs, as indicated in the figure, as well as by Rituxan.

Figure 54 provides graphs of the CDC activity induced by vawrious CD20 binding scorpions and SMIPs, as indicated in the figure, as well as by Rituxan.

Figure 55 provides histograms showing the levels of Clq binding to CD20 binding scorpions bound to Ramos B-cells.

Figure 56 provides scatter plots of FACS analyses showing the loss of mitochondrial membrane potential attributable to CD20-binding scorpions (2Lm20 4x2Lm20-4 and 011x2Lm2O-4) and Rituxan* relative to controls (upper panel); histograms of the percentage of cells with disrupted mitochondrial membrane potential (disrupted MMP: black bars) are shown in the lower panel.

Figure 57 provides histograms showing the relative lack of caspase 3 activation by CD20-binding scorpions (2Lm20-4x2Lm20-4 and 01 x2Lm20-4), Rituximab, CD95, and controls.

Figure 58 provides a composite of four Western blot analyses of Poly (ADP ribose) Polymerase and caspases 3, 7, and 9 from B-cells showing little degradation of any of these proteins attributable to CD20-binding scorpions binding to the cells.

Figure 59 is a gel electrophoretogram of B-cell chromosomal DNAs showing the degree of fragmentation attributable to CD20-binding scorpions binding to the cells.

Figure 60 is a gel electrophoretogram of immunoprecipitates obtained with each of an anti-phosphotyrosine antibody and an anti-SYK antibody. The immunoprecipitates were obtained from lysates of B-cells contacted with CD20 binding scorpions, as indicated in the figure.

Figure 61 provides combination index plots of CD20-binding scorpions in combination therapies with each of doxorubicin, vincristine and rapamycin.

DETAILED DESCRIPTION

The present invention provides compositions of relatively small peptides having at least two binding regions or domains, which may provide one or more binding specificities, derived from variable binding domains of immunoglobulins, such as antibodies, disposed terminally relative to an effector domain comprising at least part of an immunoglobulin constant region (i.e., a source from which a constant sub-region, as defined herein, may be derived), as well as nucleic acids, vectors and host cells involved in the recombinant production of such peptides and methods of using the peptide compositions in a variety of diagnostic and therapeutic applications, including the treatment of a disorder as well as the amelioration of at least one symptom of such a disorder. The peptide compositions advantageously arrange a second binding domain C-terminal to the effector domain, an arrangement that unexpectedly provides sterically unhindered or less hindered binding by at least two binding domains of the peptide, while retaining an effector function or functions of the centrally disposed effector domain.

The first and second binding domains of the multivalent peptides according to the invention may be the same (i.e., have identical or substantially identical amino acid sequences and be monospecific) or different (and be multispecific). Although different in terms of primary structure, the first and second binding domains may recognize and bind to the same epitope of a target molecule and would therefore be monospecific. In many instances, however, the binding domains will differ structurally and will bind to different binding sites, resulting in a multivalent, multispecific protein. Those different binding sites may exist on a single target molecule or on different target molecules. In the case of the two binding molecules recognizing different target molecules, those target molecules may exist, e.g., on or in the same structure (e.g., the surface of the same cell), or those target molecules may exist on or in separate structures or locales. For example, a multispecific binding protein according to the invention may have binding domains that specifically bind to target molecule on the surfaces of distinct cell types. Alternatively, one binding domain may specifically bind to a target on a cell surface and the other binding domain may specifically bind to a target not found associated with a cell, such as an extracellular structural (matrix) protein or a free (e.g., soluble or stromal) protein.

The first and second binding domains are derived from one or more regions of the same, or different, immunoglobulin protein structures such as antibody molecules. The first and/or second binding domain may exhibit a sequence identical to the sequence of a region of an immunoglobuin, or may be a modification of such a sequence to provide, e.g., altered binding properties or altered stability. Such modifications are known in the art and include alterations in amino acid sequence that contribute directly to the altered property such as altered binding, for example by leading to an altered secondary or higher order structure for the peptide, Also contemplated are modified amino acid sequences resulting from the incorporation of non-native amino acids, such as non-native conventional amino acids, unconventional amino acids and imino acids. In some embodiments, the altered sequence results in altered post-translational processing, for example leading to an altered glycosylation pattern.

Any of a wide variety of binding domains derived from an immunoglobulin or immunoglobulin-like polypeptide (e.g., receptor) are contemplated for use in scorpions. Binding domains derived from antibodies comprise the CDR regions of a VL and a VH domain, seen, e.g., in the context of using a binding domain from a humanized antibody. Binding domains comprising complete VL and VH domains derived from an antibody may be organized in either orientation. A scorpion according to the invention may have any of the binding domains herein described. For scorpions having at least one binding domain recognizing a B-cell, exemplary scorpions have at least one binding domain derived from CD3, CD10, CD19, CD20, CD21, CD22, CD23, CD24, CD37, CD38, CD39, CD40, CD72, CD73, CD74, CDw75, CDw76, CD77, CD78, CD79a/b, CD80, CD81, CD82, CD83, CD84, CD85, CD86, CD89, CD98, CD126, CD127, CDw130, CD138 or CDwl50. In some embodiments, the scorpion is a multivalent binding protein comprising at least one binding domain having a sequence selected from the group consisting of SEQ ID NOS: 2, 4, 6, 103, 105, 107 and 109. In some embodiments, a scorpion comprises a binding domain comprising a sequence selected from the group consisting of any of SEQ ID NOS: 332-345. In some embodiments, a scorpion comprises a binding domain comprising a sequence derived from immunoglobulin VL and VH domains, wherein the sequence is selected from the group consisting of any of SEQ ID NOS: 355-365. The invention further contemplates scorpions comprising a binding domain that has the opposite orientation of VL and VH having sequences deducible from any of SEQ ID NOS:355-365.

For embodiments in which either, or both, of the binding domains are derived from more than one region of an immunoglobulin (e.g., an Ig VL region and an Ig VH region), the plurality of regions may be joined by a linker peptide. Moreover, a linker may be used to join the first binding domain to a constant sub-region. Joinder of the constant sub-region to a second binding domain (i.e., binding domain 2 disposed towards the C-terminus of a scorpion) is accomplished by a scorpion linker. These scorpion linkers are preferably between about 2-45 amino acids, or 2-38 amino acids, or 5-45 amino acids. For example, the H1 linker is 2 amino acids in length and the STD2 linker is 38 amino acids in length. Beyond general length considerations, a scorpion linker region suitable for use in the scorpions according to the invention includes an antibody hinge region selected from the group consisting of IgG, IgA, IgD and IgE hinges and variants thereof. For example, the scorpion linker may be an antibody hinge region selected from the group consisting of human IgGI, human

IgG2, human IgG3, and human IgG4, and variants thereof. In some embodiments, the scorpion linker region has a single cysteine residue for formation of an interchain disulfide bond. In other embodiments, the scorpion linker has two cysteine residues for formation of interchain disulfide bonds. In some embodiments, a scorpion linker region is derived from an immunoglobulin hinge region or a C-lectin stalk region and comprises a sequence selected from the group consisting of SEQ ID NOS:111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 149, 151, 153, 155, 157, 159, 253 161,163,165,167,169,231,233,235,237,239,241, 24 3,24 5,247 ,24 9 ,25 1,

, 255,257,259,261,263,265,267,269,271,273,275,277,279,281,287,289,297, 305, 307, 309, 310, 311, 313, 314, 315, 316,317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 346, 351, 352, 353, 354, 373, 374, 375, 376 and 377. More generally, any sequence of amino acids identified in the sequence listing as providing a sequence derived from a hinge region is contemplated for use as a scorpion linker in the scorpion molecules according to the invention. In addition, a scorpion linker derived from an Ig hinge is a hinge-like peptide domain having at least one free cysteine capable of participating in an interchain disulfide bond. Preferably, a scorpion linker derived from an Ig hinge peptide retains a cysteine that corresponds to the hinge cysteine disposed towards the N-terminus of that hinge. Preferably, a scorpion linker derived from an IgG1 hinge has one cysteine or has two cysteines corresponding to hinge cysteines. Additionally, a scorpion linker is a stalk region of a Type II C-lectin molecule. In some embodiments, a scorpion comprises a scorpion linker having a sequence selected from the group consisting of SEQ ID NOS:373-377.

The centrally disposed constant sub-region is derived from a constant region of an immunoglobulin protein. The constant sub-region generally is derived from a CH2 portion of a CH region of an immunoglobulin in the abstract, although it may be derived from a CH2-CH3 portion. Optionally, the constant sub-region may be derived from a hinge-CH2 or hinge-CH2-CH3 portion of an immunoglobulin, placing a peptide corresponding to an Ig hinge region N-terminal to the constant sub-region and disposed between the constant sub-region and binding domain 1. Also, portions of the constant sub-region may be derived from the CH regions of different immunoglobulins. Further, the peptide corresponding to an Ig CH3 may be truncated, leaving a C-terminal amino acid sequence selected from the group consisting of SEQ ID NOS:366-371. It is preferred, however, that in embodiments in which a scorpion hinge is a hinge-like peptide derived from an immunoglobulin hinge, that the scorpion linker and the constant sub-region be derived from the same type of immunoglobulin. The constant sub-region provides at least one activity associated with a CH region of an immunoglobulin, such as antibody-dependent cell-mediated cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), protein A binding, binding to at least one FC receptor, reproducibly detectable stability relative to a protein according to the invention except for the absence of a constant sub-region, and perhaps placental transfer where generational transfer of a molecule according to the invention would be advantageous, as recognized by one of skill in the art. As with the above-described binding domains, the constant sub-region is derived from at least one immunoglobulin molecule and exhibits an identical or substantially identical amino acid sequence to a region or regions of at least one immunoglobulin. In some embodiments, the constant sub-region is modified from the sequence or sequences of at least one immunoglobulin (by substitution of one or more non-native conventional or unconventional, e.g., synthetic, amino acids or imino acids), resulting in a primary structure that may yield an altered secondary or higher order structure with altered properties associated therewith, or may lead to alterations in post-translational processing, such as glycosylation.

For those binding domains and constant sub-regions exhibiting an identical or substantially identical amino acid sequence to one or more immunoglobulin polypeptides, the post-translational modifications of the molecule according to the invention may result in a molecule modified relative to the immunoglobulin(s) serving as a basis for modification. For example, using techniques known in the art, a host cell may be modified, e.g. a CHO cell, in a manner that leads to an altered polypeptide glycosylation pattern relative to that polypeptide in an unmodified (e.g., CHO) host cell.

Provided with such molecules, and the methods of recombinantly producing them in vivo, new avenues of targeted diagnostics and therapeutics have been opened to allow, e.g., for the targeted recruitment of effector cells of the immune system (e.g., cytotoxic T lymphocytes, natural killer cells, and the like) to cells, tissues, agents and foreign objects to be destroyed or sequestered, such as cancer cells and infectious agents. In addition to localizing therapeutic cells to a site of treatment, the peptides are useful in localizing therapeutic compounds, such as radiolabeled proteins. Further, the peptides are also useful in scavenging deleterious compositions, for example by associating a deleterious composition, such as a toxin, with a cell capable of destroying or eliminating that toxin (e.g., a macrophage). The molecules of the invention are useful in modulating the activity of binding partner molecules, such as cell surface receptors. This is shown in Figure 17 where apoptotic signaling through CD20 and/or CD37 is markedly enhanced by a molecule of the present invention. The effect of this signaling is the death of the targeted cell. Diseases and conditions where the elimination of defined cell populations is beneficial would include infectious and parasitic diseases, inflammatory and autoimmune conditions, malignancies, and the like. One skilled in the art would recognize that there is no limitation of the approach to the enhancement of apoptotic signaling. Mitotic signaling and signaling leading to differentiation, activation, or inactivation of defined cell populations can be induced by molecules of the present invention through the appropriate selection of binding partner molecules. Further consideration of the disclosure of the invention will be facilitated by a consideration of the following express definitions of terms used herein.

A "single-chain binding protein" is a single contiguous arrangement of covalently linked amino acids, with the chain capable of specifically binding to one or more binding partners sharing sufficient determinants of a binding site to be detectably bound by the single-chain binding protein. Exemplary binding partners include proteins, carbohydrates, lipids and small molecules.

For ease of exposition, "derivatives" and "variants" of proteins, polypeptides, and peptides according to the invention are described in terms of differences from proteins and/or polypeptides and/or peptides according to the invention, meaning that the derivatives and variants, which are proteins/polypeptides/peptides according to the invention, differ from underivatized or non-variant proteins, polypeptides or peptides of the invention in the manner defined. One of skill in the art would understand that the derivatives and variants themselves are proteins, polypeptides and peptides according to the invention.

An "antibody" is given the broadest definition consistent with its meaning in the art, and includes proteins, polypeptides and peptides capable of binding to at least one binding partner, such as a proteinaceous or non-proteinaceous antigen. An "antibody" as used herein includes members of the immunoglobulin superfamily of proteins, of any species, of single- or multiple-chain composition, and variants, analogs, derivatives and fragments of such molecules. Specifically, an "antibody" includes any form of antibody known in the art, including but not limited to, monoclonal and polyclonal antibodies, chimeric antibodies, CDR-grafted antibodies, humanized antibodies, single-chain variable fragments, bi-specific antibodies, diabodies, antibody fusions, and the like.

A "binding domain" is a peptide region, such as a fragment of a polypeptide derived from an immunoglobulin (e.g., an antibody), that specifically binds one or more specific binding partners. If a plurality of binding partners exists, those partners share binding determinants sufficient to detectably bind to the binding domain. Preferably, the binding domain is a contiguous sequence of amino acids.

An "epitope" is given its ordinary meaning herein of a single antigenic site, i.e., an antigenic determinant, on a substance (e.g., a protein) with which an antibody specifically interacts, for example by binding. Other terms that have acquired well settled meanings in the immunoglobulin (e.g., antibody) art, such as a "variable light region," variable heavy region," "constant light region," constant heavy region," "antibody hinge region," "complementarity determining region," "framework region," "antibody isotype," "Fc region," "single-chain variable fragment" or "scFv," "diabody," "chimera," "CDR-grafted antibody," "humanized antibody," "shaped antibody," "antibody fusion," and the like, are each given those well-settled meanings known in the art, unless otherwise expressly noted herein.

Terms understood by those in the art as referring to antibody technology are each given the meaning acquired in the art, unless expressly defined herein. Examples of such terms are "V"and"VH", referring to the variable binding region derived from an antibody light and heavy chain, respectively; and CL and CH, referring to an "immunoglobulin constant region," i.e., a constant region derived from an antibody light or heavy chain, respectively, with the latter region understood to be further divisible into CH, CH2, CH3 and CH4 constant region domains, depending on the antibody isotype (IgA, IgD, IgE, IgG, IgM) from which the region was derived. CDR means "complementarity determining region." A "hinge region" is derived from the amino acid sequence interposed between, and connecting, the CH and C H2 regions of a single chain of an antibody, which is known in the art as providing flexibility, in the form of a "hinge," to whole antibodies.

A "constant sub-region" is a term defined herein to refer to a peptide, polypeptide, or protein sequence that corresponds to, or is derived from, one or more constant region domains of an antibody. Thus, a constant sub-region may include any or all of the following domains: a CH1 domain, a hinge region, a CH2 domain, a Cf3 domain (IgA, IgD, IgG, IgE, and IgM), and a CH4 domain (IgE, IgM). A constant sub-region as defined herein, therefore, can refer to a polypeptide region corresponding to an entire constant region of an antibody, or a portion thereof. Typically, a constant sub-region of a polypeptide, or encoding nucleic acid, of the invention has a hinge, CH2 domain, and CH3 domain.

An "effector function" is a function associated with or provided by a constant region of an antibody. Exemplary effector functions include antibody-dependent cell mediated cytotoxicity (ADCC), complement activation and complement-dependent cytotoxicity (CDC), Fc receptor binding, and increased plasma half-life, as well as placental transfer. An effector function of a composition according to the invention is detectable; preferably, the specific activity of the composition according to the invention for that function is about the same as the specific activity of a wild-type antibody with respect to that effector function, i.e., the constant sub-region of the multivalent binding molecule preferably has not lost any effector function relative to a wild-type antibody]

A "linker" is a peptide, or polynucleotide, that joins or links other peptides or polynucleotides. Typically, a peptide linker is an oligopeptide of from about 2-50 amino acids, with typical polynucleotide linkers encoding such a peptide linker and, thus, being about 6-150 nucleotides in length. Linkers join the first binding domain to a constant sub-region domain. An exemplary peptide linker is (Gly 4Ser) 3 . A scorpion linker is used to join the C-terminal end of a constant sub-region to a second binding domain. The scorpion linker may be derived from an immunoglobulin hinge region or from the stalk region of a type II C-lectin, as described in greater detail below.

A "target" is given more than one meaning, with the context of usage defining an unambiguous meaning in each instance. In its narrowest sense, a "target" is a binding site, i.e., the binding domain of a binding partner for a peptide composition according to the invention. In a broader sense, "target" or "molecular target" refers to the entire binding partner (e.g., a protein), which necessarily exhibits the binding site. Specific targets, such as "CD20," "CD37," and the like, are each given the ordinary meaning the term has acquired in the art. A "target cell" is any prokaryotic or eukaryotic cell, whether healthy or diseased, that is associated with a target molecule according to the invention. Of course, target molecules are also found unassociated with any cell (i.e., a cell-free target) or in association with other compositions such as viruses (including bacteriophage), organic or inorganic target molecule carriers, and foreign objects.

Examples of materials with which a target molecule may be associated include autologous cells (e.g., cancer cells or other diseased cells), infectious agents (e.g., infectious cells and infectious viruses), and the like. A target molecule may be associated with an enucleated cell, a cell membrane, a liposome, a sponge, a gel, a capsule, a tablet, and the like, which may be used to deliver, transport or localize a target molecule, regardless of intended use (e.g., for medical treatment, as a result of benign or unintentional provision, or to further a bioterrorist threat). "Cell-free," "virus-free," "carrier-free," "object-free," and the like refer to target molecules that are not associated with the specified composition or material.

"Binding affinity" refers to the strength of non-covalent binding of the peptide compositions of the invention and their binding partners. Preferably, binding affinity refers to a quantitative measure of the attraction between members of a binding pair.

An "adjuvant" is a substance that increases or aids the functional effect of a compound with which it is in association, such as in the form of a pharmaceutical composition comprising an active agent and an adjuvant. An "excipient" is an inert substance used as a diluent in formulating a pharmaceutical composition. A "carrier" is a typically inert substance used to provide a vehicle for delivering a pharmaceutical composition.

"Host cell" refers to any cell, prokaryotic or eukaryotic, in which is found a polynucleotide, protein or peptide according to the invention.

"Introducing" a nucleic acid or polynucleotide into a host cell means providing for entry of the nucleic acid or polynucleotide into that cell by any means known in the art, including but not limited to, in vitro salt-mediated precipitations and other forms of transformation of naked nucleic acid/polynucleotide or vector-borne nucleic acid/polynucleotide, virus-mediated infection and optionally transduction, with or without a "helper" molecule, ballistic projectile delivery, conjugation, and the like.

"Incubating" a host cell means maintaining that cell under environmental conditions known in the art to be suitable for a given purpose, such as gene expression. Such conditions, including temperature, ionic strength, oxygen tension, carbon dioxide concentration, nutrient composition, and the like, are well known in the art.

"Isolating" a compound, such as a protein or peptide according to the invention, means separating that compound from at least one distinct compound with which it is found associated in nature, such as in a host cell expressing the compound to be isolated, e.g. by isolating spent culture medium containing the compound from the host cells grown in that medium.

An "organism in need" is any organism at risk of, or suffering from, any disease, disorder or condition that is amenable to treatment or amelioration with a composition according to the invention, including but not limited to any of various forms of cancer, any of a number of autoimmune diseases, radiation poisoning due to radiolabeled proteins, peptides and like compounds, ingested or internally produced toxins, and the like, as will become apparent upon review of the entire disclosure. Preferably, an organism in need is a human patient.

"Ameliorating" a symptom of a disease means detectably reducing the severity of that symptom of disease, as would be known in the art. Exemplary symptoms include pain, heat, swelling and joint stiffness.

Unless clear from context, the terms "protein," "peptide," and "polypeptide" are used interchangeably herein, with each referring to at least one contiguous chain of amino acids. Analogously, the terms "polynucleotide," "nucleic acid," and "nucleic acid molecule" are used interchangeably unless it is clear from context that a particular, and non-interchangeable, meaning is intended.

"Pharmaceutically acceptable salt" refers to salts of the compounds of the present invention derived from the combination of such compounds and an organic or inorganic acid (acid addition salts) or an organic or inorganic base (base addition salts).

Using the terms as defined above, a general description of the various aspects of the invention is provided below. Following the general description, working examples are presented to provide supplementary evidence of the operability and usefulness of the invention disclosed herein.

Proteins and polypeptides

In certain embodiments of the invention, there are provided any of the herein described multivalent binding proteins with effector function, including binding domain-immunoglobulin fusion proteins, wherein the multivalent binding protein or peptide with effector function comprises two or more binding domain polypeptide sequences. Each of the binding domain polypeptide sequences is capable of binding or specifically binding to a target(s), such as an antigen(s), which target(s) or antigen(s) may be the same or may be different. The binding domain polypeptide sequence may be derived from an antigen variable region or it may be derived from immunoglobulin-like molecules, e.g., receptors that fold in ways that mimic immunoglobulin molecules. The antibodies from which the binding domains are derived may be antibodies that are polyclonal, including monospecific polyclonal, monoclonal (mAbs), recombinant, chimeric, humanized (such as CDR-grafted), human, single-chain, catalytic, and any other form of antibody known in the art, as well as fragments, variants or derivatives thereof. In some embodiments, each of the binding domains of the protein according to the invention is derived from a complete variable region of an immunoglobulin. In preferred embodiments, the binding domains are each based on a human Ig variable region. In other embodiments, the protein is derived from a fragment of an Ig variable region. In such embodiments, it is preferred that each binding domain polypeptide sequence correspond to the sequences of each of the complementarity determining regions of a given Ig variable region. Also contemplated within the invention are binding domains that correspond to fewer than all CDRs of a given Ig variable region, provided that such binding domains retain the capacity to specifically bind to at least one target.

The multivalent binding protein with effector function also has a constant sub region sequence derived from an immunoglobulin constant region, preferably an antibody heavy chain constant region, covalently juxtaposed between the two binding domains in the multivalent binding protein with effector function.

The multivalent binding protein with effector function also has a scorpion linker that joins the C-terminal end of the constant sub-region to the N-terminal end of binding domain 2. The scorpion linker is not a helical peptide and may be derived from an antibody hinge region, from a region connecting binding domains of an immunoglobulin, or from the stalk region of type II C-lectins. The scorpion linker may be derived from a wild-type hinge region of an immunoglobulin, such as an IgG1, IgG2, IgG3, IgG4, IgA, IgD or an IgE hinge region. In other embodiments, the invention provides multivalent binding proteins with altered hinges. One category of altered hinge regions suitable for inclusion in the multivalent binding proteins is the category of hinges with an altered number of Cysteine residues, particularly those Cys residues known in the art to be involved in interchain disulfide bond formation in immunoglobulin counterpart molecules having wild-type hinges. Thus, proteins may have an IgG Ihinge in which one of the three Cys residues capable of participating in interchain disulfide bond formations is missing. To indicate the Cysteine sub structure of altered hinges, the Cys subsequence is presented from N- to C-terminus. Using this identification system, the multivalent binding proteins with altered IgG hinges include hinge structures characterized as cxc, xxc, ccx, xxc, xcx, cxx, and xxx. The Cys residue may be either deleted or substituted by an amino acid that results in a conservative substitution or a non-conservative substitution. In some embodiments, the Cysteine is replaced by a Serine. For proteins with scorpion linkers comprising IgG Ihinges, the number of cysteines corresponding to hinge cysteines is reduced to 1 or 2, preferably with one of those cysteines corresponding to the hinge cysteine disposed closest to the N-terminus of the hinge.

For proteins with scorpion linkers comprising IgG2 hinges, there may be 0, 1, 2, 3, or 4 Cys residues. Forscorpion linkers comprising altered IgG2 hinges containing 1, 2 or 3 Cys residues, all possible subsets of Cys residues are contemplated. Thus, for such linkers having one Cys, the multivalent binding proteins may have the following Cys motif in the hinge region: cxxx, xcxx, xxcx, or xxxc. For scorpion linkers comprising IgG2 hinge variants having 2 or 3 Cys residues, all possible combinations of retained and substituted (or deleted) Cys residues are contemplated. For multivalent binding proteins with scorpion linkers comprising altered IgG3 or altered IgG4 hinge regions, a reduction in Cys residues from 1 to one less than the complete number of Cys residues in the hinge region is contemplated, regardless of whether the loss is through deletion or substitution by conservative or non-conservative amino acids (e.g., Serine). In like manner, multivalent binding proteins having a scorpion linker comprising a wild-type IgA, IgD or IgE hinge are contemplated, as are corresponding altered hinge regions having a reduced number of Cys residues extending from 0 to one less than the total number of Cys residues found in the corresponding wild-type hinge. In some embodiments having an IgGl hinge, the first, or N-terminal, Cys residue of the hinge is retained. For proteins with either wild-type or altered hinge regions, it is contemplated that the multivalent binding proteins will be single-chain molecules capable of forming homo multimers, such as dimers, e.g., by disulfide bond formation. Further, proteins with altered hinges may have alterations at the termini of the hinge region, e.g., loss or substitution of one or more amino acid residues at the N-terminus, C-terminus or both termini of a given region or domain, such as a hinge domain, as disclosed herein.

In another exemplary embodiment, the constant sub-region is derived from a constant region that comprises a native, or an engineered, IgD hinge region. The wild-type human IgD hinge has one cysteine that forms a disulfide bond with the light chain in the native IgD structure. In some embodiments, this IgD hinge cysteine is mutated (e.g., deleted) to generate an altered hinge for use as a connecting region between binding domains of, for example, a bispecific molecule. Other amino acid changes or deletions or alterations in an IgD hinge that do not result in undesired hinge inflexibility are within the scope of the invention. Native or engineered IgD hinge regions from other species are also within the scope of the invention, as are humanized native or engineered IgD hinges from non-human species, and (other non

IgD) hinge regions from other human, or non-human, antibody isotypes, (such as the llama IgG2 hinge).

The invention further comprehends constant sub-regions attached to scorpion linkers that may be derived from hinges that correspond to a known hinge region, such as an IgG I hinge or an IgD hinge, as noted above. The constant sub-region may contain a modified or altered (relative to wild-type) hinge region in which at least one cysteine residue known to participate in inter-chain disulfide bond linkage is replaced by another amino acid in a conservative substitution (e.g., Ser for Cys) or a non conservative substitution. The constant sub-region does not include a peptide region or domain that corresponds to an immunoglobulin CHI domain.

Alternative hinge and linker sequences that can be used as connecting regions are from portions of cell surface receptors that connect immunoglobulin V-like or immunoglobulin C-like domains. Regions between Ig V-like domains where the cell surface receptor contains multiple Ig V-like domains in tandem, and between Ig C like domains where the cell surface receptor contains multiple tandem Ig C-like regions are also contemplated as connecting regions. Hinge and linker sequences are typically from 5 to 60 amino acids long, and may be primarily flexible, but may also provide more rigid characteristics. In addition, linkers frequently provide spacing that facilitates minimization of steric hindrance between the binding domains. Preferably, these hinge and linker peptides are primarily a helical in structure, with minimal sheet structure. The preferred sequences are stable in plasma and serum and are resistant to proteolytic cleavage. The preferred sequences may contain a naturally occurring or added motif such as the CPPC motif that confers a disulfide bond to stabilize dimer formation. The preferred sequences may contain one or more glycosylation sites. Examples of preferred hinge and linker sequences include, but are not limited to, the interdomain regions between the Ig V-like and Ig C-like regions of CD2, CD4, CD22, CD33, CD48, CD58, CD66, CD80, CD86, CD150, CD166, and CD244.

The constant sub-region may be derived from a camelid constant region, such as either a llama or camel IgG2 or IgG3.

Specifically contemplated is a constant sub-region having the CH2-CH3 region from any Ig class, or from any IgG subclass, such as IgG I(e.g., human IgG1). In preferred embodiments, the constant sub-region and the scorpion linker derived from an immunoglobulin hinge are both derived from the same Ig class. In other preferred embodiments, the constant sub-region and the scorpion linker derived from an immunoglobulin hinge are both derived from the same Ig sub-class. The constant sub-region also may be a CH3 domain from any Ig class or subclass, such as IgG1 (e.g., human IgG1), provided that it is associated with at least one immunoglobulin effector function.

The constant sub-region does not correspond to a complete immunoglobulin constant region (i.e., CH1-hinge-CH2-Cm) of the IgG class. The constant sub-region may correspond to a complete immunoglobulin constant region of other classes., IgA constant domains, such as an IgA1 hinge, an IgA2 hinge, an IgA CH2 and an IgA CH3 domains with a mutated or missing tailpiece are also contemplated as constant sub regions. Further, any light chain constant domain may function as a constant sub region, e.g., CK or any CL. The constant sub-region may also include JH or JK, with or without a hinge. The constant sub-region may also correspond to engineered antibodies in which, e.g., a loop graft has been constructed by making selected amino acid substitutions using an IgG framework to generate a binding site for a receptor other than a natural FcR (CD16, CD32, CD64, FcER1), as would be understood in the art. An exemplary constant sub-region of this type is an IgG CH2-CH3 region modified to have a CD89 binding site.

This aspect of the invention provides a multivalent binding protein or peptide having effector function, comprising, consisting essentially of, or consisting of (a) an N-terminally disposed binding domain polypeptide sequence derived from an immunoglobulin that is fused or otherwise connected to (b) a constant sub-region polypeptide sequence derived from an immunoglobulin constant region, which preferably includes a hinge region sequence, wherein the hinge region polypeptide may be as described herein, and may comprise, consist essentially of, or consist of, for example, an alternative hinge region polypeptide sequence, in turn fused or otherwise connected to (c) a C-terminally disposed second native or engineered binding domain polypeptide sequence derived from an immunoglobulin.

The centrally disposed constant sub-region polypeptide sequence derived from an immunoglobulin constant region is capable of at least one immunological activity selected from the group consisting of antibody dependent cell-mediated cytotoxicity, CDC, complement fixation, and Fc receptor binding, and the binding domain polypeptides are each capable of binding or specifically binding to a target, such as an antigen, wherein the targets may be the same or different, and may be found in effectively the same physiological environment (e.g., the surface of the same cell) or in different environments (e.g., different cell surfaces, a cell surface and a cell-free location, such as in solution).

This aspect of the invention also comprehends variant proteins or polypeptides exhibiting an effector function that are at least 80%, and preferably 85%, 90%, 95% or 99% identical to a multivalent protein with effector function of specific sequence as disclosed herein.

Polynucleotides

The invention also provides polynucleotides (isolated or purified or pure polynucleotides) encoding the proteins or peptides according to the invention, vectors (including cloning vectors and expression vectors) comprising such polynucleotides, and cells (e.g., host cells) transformed or transfected with a polynucleotide or vector according to the invention. In encoding the proteins or polypeptides of the invention, the polynucleotides encode a first binding domain, a second binding domain and an Fc domain, all derived from immunoglobulins, preferably human immunoglobulins. Each binding domain may contain a sequence corresponding to a full-length variable region sequence (either heavy chain and/or light chain), or to a partial sequence thereof, provided that each such binding domain retains the capacity to specifically bind. The Fc domain may have a sequence that corresponds to a full-length immunoglobulin FC domain sequence or to a partial sequence thereof, provided that the Fc domain exhibits at least one effector function as defined herein. In addition, each of the binding domains may be joined to the Fc domain via a linker peptide that typically is at least 8, and preferably at least 13, amino acids in length. A preferred linker sequence is a sequence based on the Gly4 Ser motif, such as (Gly4 Ser) 3 .

Variants of the multivalent binding protein with effector function are also comprehended by the invention. Variant polynucleotides are at least 90%, and preferably 95%, 99%, or 99.9% identical to one of the polynucleotides of defined sequence as described herein, or that hybridizes to one of those polynucleotides of defined sequence under stringent hybridization conditions of 0.015 M sodium chloride, 0.0015 M sodium citrate at 65-68°C or 0.015 M sodium chloride, 0.0015M sodium citrate, and 50% formamide at 420 C. The polynucleotide variants retain the capacity to encode a multivalent binding protein with effector function.

The term "stringent" is used to refer to conditions that are commonly understood in the art as stringent. Hybridization stringency is principally determined by temperature, ionic strength, and the concentration of denaturing agents such as formamide. Examples of stringent conditions for hybridization and washing are 0.015 M sodium chloride, 0.0015 M sodium citrate at 65-68°C or 0.015 M sodium chloride, 0.0015M sodium citrate, and 50% formamide at 42°C. See Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, (Cold Spring Harbor, N.Y. 1989).

More stringent conditions (such as higher temperature, lower ionic strength, higher formamide, or other denaturing agent) may also be used; however, the rate of hybridization will be affected. In instances wherein hybridization of deoxyoligonucleotides is concerned, additional exemplary stringent hybridization conditions include washing in 6x SSC, 0.05% sodium pyrophosphate at 37°C (for 14 base oligonucleotides), 48°C (for 17-base oligonucleotides), 55°C (for 20-base oligonucleotides), and 60°C (for 23-base oligonucleotides).

In a related aspect of the invention, there is provided a method of producing a polypeptide or protein or other construct of the invention, for example, including a multivalent binding protein or peptide having effector function, comprising the steps of (a) culturing a host cell as described or provided for herein under conditions that permit expression of the construct; and (b) isolating the expression product, for example, the multivalent binding protein or peptide with effector function from the host cell or host cell culture.

Constructs

The present invention also relates to vectors, and to constructs prepared from known vectors, that each include a polynucleotide or nucleic acid of the invention, and in particular to recombinant expression constructs, including any of various known constructs, including delivery constructs, useful for gene therapy, that include any nucleic acids encoding multivalent, for example, multispecific, including bi specific, binding proteins and polypeptides with effector function, as provided herein; to host cells which are genetically engineered with vectors and/or other constructs of the invention and to methods of administering expression or other constructs comprising nucleic acid sequences encoding multivalent, for example, multispecific, including bi-specific, binding proteins with effector function, or fragments or variants thereof, by recombinant techniques.

Various constructs of the invention including multivalent, for example, multispecific binding proteins with effector function, can be expressed in virtually any host cell, including in vivo host cells in the case of use for gene therapy, under the control of appropriate promoters, depending on the nature of the construct (e.g., type of promoter, as described above), and on the nature of the desired host cell (e.g., postmitotic terminally differentiated or actively dividing; e.g., maintenance of an expressible construct as an episome or integrated into the host cell genome).

Appropriate cloning and expression vectors for use with prokaryotic and eukaryotic hosts are described, for example, in Sambrook, et al., Molecular Cloning: A LaboratoryManual, Second Edition, Cold Spring Harbor, NY, (1989). Exemplary cloning/expression vectors include, but are not limited to, cloning vectors, shuttle vectors, and expression constructs, that may be based on plasmids, phagemids, phasmids, cosmids, viruses, artificial chromosomes, or any nucleic acid vehicle suitable for amplification, transfer, and/or expression of a polynucleotide contained therein that is known in the art. As noted herein, in preferred embodiments of the invention, recombinant expression is conducted in mammalian cells that have been transfected, transformed or transduced with a nucleic acid according to the invention. See also, for example, Machida, CA., "Viral Vectors for Gene Therapy: Methods and Protocols"; Wolff, JA, "Gene Therapeutics: Methods and Applications of Direct Gene Transfer" (Birkhauser 1994); Stein, U and Walther, W (eds., "Gene Therapy of Cancer: Methods and Protocols" (Humana Press 2000); Robbins, PD (ed.), "Gene Therapy Protocols" (Humana Press 1997); Morgan, JR (ed.), "Gene Therapy Protocols" (Humana Press 2002); Meager, A (ed.), "Gene Therapy Technologies,

Applications and Regulations: From Laboratory to Clinic" (John Wiley & Sons Inc. 1999); MacHida, CA and Constant, JG, "Viral Vectors for Gene Therapy: Methods and Protocols" (Humana Press 2002);"New Methods Of Gene Therapy For Genetic Metabolic Diseases NIH Guide," Volume 22, Number 35, October 1, 1993. See also U.S. Pat. Nos. 6,384,210; 6,384,203; 6,384,202; 6,384,018; 6,383,814; 6,383,811; 6,383,795; 6,383,794; 6,383,785; 6,383,753; 6,383,746; 6,383,743; 6,383,738; 6,383,737; 6,383,733; 6,383,522; 6,383,512; 6,383,481; 6,383,478; 6,383,138; 6,380,382; 6,380,371; 6,380,369; 6,380,362; 6,380,170; 6,380,169; 6,379,967; and 6,379,966.

Typically, expression constructs are derived from plasmid vectors. One preferred construct is a modified pNASS vector (Clontech, Palo Alto, CA), which has nucleic acid sequences encoding an ampicillin resistance gene, a polyadenylation signal and a T7 promoter site. Other suitable mammalian expression vectors are well known (see, e.g., Ausubel et al., 1995; Sambrook et al., supra;see also, e.g., catalogues from Invitrogen, San Diego, CA; Novagen, Madison, WI; Pharmacia, Piscataway, NJ). Presently preferred constructs may be prepared that include a dihydrofolate reductase (DHFR)-encoding sequence under suitable regulatory control, for promoting enhanced production levels of the multivalent binding protein with effector function, which levels result from gene amplification following application of an appropriate selection agent (e.g., methotrexate).

Generally, recombinant expression vectors will include origins of replication and selectable markers permitting transformation of the host cell, and a promoter derived from a highly-expressed gene to direct transcription of a downstream structural sequence, as described above. A vector in operable linkage with a polynucleotide according to the invention yields a cloning or expression construct. Exemplary cloning/expression constructs contain at least one expression control element, e.g., a promoter, operably linked to a polynucleotide of the invention. Additional expression control elements, such as enhancers, factor-specific binding sites, terminators, and ribosome binding sites are also contemplated in the vectors and cloning/expression constructs according to the invention. The heterologous structural sequence of the polynucleotide according to the invention is assembled in appropriate phase with translation initiation and termination sequences. Thus, for example, the multivalent binding protein-encoding nucleic acids as provided herein may be included in any one of a variety of expression vector constructs as a recombinant expression construct for expressing such a protein in a host cell. In certain preferred embodiments the constructs, are included in formulations that are administered in vivo. Such vectors and constructs include chromosomal, nonchromosomal and synthetic DNA sequences, e.g., derivatives of SV40; bacterial plasmids; phage DNA; yeast plasmids; vectors derived from combinations of plasmids and phage DNA, viral DNA, such as vaccinia, adenovirus, fowl pox virus, and pseudorabies, or replication deficient retroviruses as described below. However, any other vector may be used for preparation of a recombinant expression construct, and in preferred embodiments such a vector will be replicable and viable in the host.

The appropriate DNA sequence(s) may be inserted into a vector, for example, by a variety of procedures. In general, a DNA sequence is inserted into an appropriate restriction endonuclease cleavage site(s) by procedures known in the art. Standard techniques for cloning, DNA isolation, amplification and purification, for enzymatic reactions involving DNA ligase, DNA polymerase, restriction endonucleases and the like, and various separation techniques are contemplated. A number of standard techniques are described, for example, in Ausubel et al. (1993 CurrentProtocolsin MolecularBiology, Greene Publ. Assoc. Inc. & John Wiley

& Sons, Inc., Boston, MA); Sambrook et al. (1989 Molecular Cloning, Second Ed., Cold Spring Harbor Laboratory, Plainview, NY); Maniatis et al. (1982 Molecular Cloning, Cold Spring Harbor Laboratory, Plainview, NY); Glover (Ed.) (1985 DNA Cloning Vol. I and II, IRL Press, Oxford, UK); Hames and Higgins (Eds.), (1985 Nucleic Acid Hybridization, IRL Press, Oxford, UK); and elsewhere.

The DNA sequence in the expression vector is operatively linked to at least one appropriate expression control sequence (e.g, a constitutive promoter or a regulated promoter) to direct mRNA synthesis. Representative examples of such expression control sequences include promoters of eukaryotic cells or their viruses, as described above. Promoter regions can be selected from any desired gene using CAT (chloramphenicol transferase) vectors or other vectors with selectable markers. Eukaryotic promoters include CMV immediate early, HSV thymidine kinase, early and late SV40, LTRs from retrovirus, and mouse metallothionein-I. Selection of the appropriate vector and promoter is well within the level of ordinary skill in the art, and preparation of certain particularly preferred recombinant expression constructs comprising at least one promoter or regulated promoter operably linked to a nucleic acid encoding a protein or polypeptide according to the invention is described herein.

Transcription of the DNA encoding proteins and polypeptides of the invention by higher eukaryotes may be increased by inserting an enhancer sequence into the vector. Examples include the SV40 enhancer on the late side of the replication origin bp 100 to 270, a cytomegalovirus early promoter enhancer, the polyoma enhancer on the late side of the replication origin, and adenovirus enhancers,

Gene therapies using the nucleic acids of the invention are also contemplated, comprising strategies to replace defective genes or add new genes to cells and/or tissues, and is being developed for application in the treatment of cancer, the correction of metabolic disorders and in the field of immunotherapy. Gene therapies of the invention include the use of various constructs of the invention, with or without a separate carrier or delivery vehicle or constructs, for treatment of the diseases, disorders, and/or conditions noted herein. Such constructs may also be used as vaccines for treatment or prevention of the diseases, disorders, and/or conditions noted herein. DNA vaccines, for example, make use of polynucleotides encoding immunogenic protein and nucleic acid determinants to stimulate the immune system against pathogens or tumor cells, Such strategies can stimulate either acquired or innate immunity or can involve the modification of immune function through cytokine expression. In vivo gene therapy involves the direct injection of genetic material into a patient or animal, typically to treat, prevent or ameliorate a disease or symptoms associated with a disease. Vaccines and immune modulation are systemic therapies. With tissue-specific in vivo therapies, such as those that aim to treat cancer, localized gene delivery and/or expression/targeting systems are preferred. Diverse gene therapy vectors that target specific tissues are known in the art, and procedures have been developed to physically target specific tissues, for example, using catheter based technologies, all of which are contemplated herein.

Ex vivo approaches to gene therapy are also contemplated herein and involve the removal, genetic modification, expansion and re-administration of a subject's, e.g., human patient's, own cells. Examples include bone marrow transplantation for cancer treatment or the genetic modification of lymphoid progenitor cells. Ex vivo gene therapy is preferably applied to the treatment of cells that are easily accessible and can survive in culture during the gene transfer process (such as blood or skin cells).

Useful gene therapy vectors include adenoviral vectors, lentiviral vectors, Adeno-associated virus (AAV) vectors, Herpes Simplex Virus (HSV) vectors, and retroviral vectors. Gene therapies may also be carried out using "naked DNA," liposome-based delivery, lipid-based delivery (including DNA attached to positively charged lipids), electroporation, and ballistic projection.

In certain embodiments, including but not limited to gene therapy embodiments, the vector may be a viral vector such as, for example, a retroviral vector. Miller et al., 1989 BioTechniques 7:980; Coffin and Varmus, 1996 Retroviruses, Cold Spring HarborLaboratoryPress,NY. For example, retroviruses from which the retroviral plasmid vectors may be derived include, but are not limited to, Moloney Murine Leukemia Virus, spleen necrosis virus, retroviruses such as Rous Sarcoma Virus, Harvey Sarcoma virus, avian leukosis virus, gibbon ape leukemia virus, human immunodeficiency virus, adenovirus, Myeloproliferative Sarcoma Virus, and mammary tumor virus.

Retroviruses are RNA viruses which can replicate and integrate into the genome of a host cell via a DNA intermediate. This DNA intermediate, or provirus, may be stably integrated into the host cell DNA. According to certain embodiments of the present invention, an expression construct may comprise a retrovirus into which a foreign gene that encodes a foreign protein is incorporated in place of normal retroviral RNA. When retroviral RNA enters a host cell coincident with infection, the foreign gene is also introduced into the cell, and may then be integrated into host cell DNA as if it were part of the retroviral genome. Expression of this foreign gene within the host results in expression of the foreign protein.

Most retroviral vector systems that have been developed for gene therapy are based on murine retroviruses. Such retroviruses exist in two forms, as free viral particles referred to as virions, or as proviruses integrated into host cell DNA. The virion form of the virus contains the structural and enzymatic proteins of the retrovirus (including the enzyme reverse transcriptase), two RNA copies of the viral genome, and portions of the source cell plasma membrane containing viral envelope glycoprotein. The retroviral genome is organized into four main regions: the Long Terminal Repeat (LTR), which contains cis-acting elements necessary for the initiation and termination of transcription and is situated both 5' and 3' to the coding genes, and the three genes encoding gag, pol, and env. These three genes, gag, pol, and env, encode, respectively, internal viral structures, enzymatic proteins (such as integrase), and the envelope glycoprotein (designated gp70 and p15e) which confers infectivity and host range specificity of the virus, as well as the "R" peptide of undetermined function.

Separate packaging cell lines and vector-producing cell lines have been developed because of safety concerns regarding the uses of retroviruses, including uses in expression constructs. Briefly, this methodology employs the use of two components, a retroviral vector and a packaging cell line (PCL). The retroviral vector contains long terminal repeats (LTRs), the foreign DNA to be transferred and a packaging sequence (y). This retroviral vector will not reproduce by itself because the genes which encode structural and envelope proteins are not included within the vector genome. The PCL contains genes encoding the gag, pol, and env proteins, but does not contain the packaging signal "y." Thus, a PCL can only form empty virion particles by itself. Within this general method, the retroviral vector is introduced into the PCL, thereby creating a vector-producing cell line (VCL). This VCL manufactures virion particles containing only the foreign genome of the retrovira vector, and therefore has previously been considered to be a safe retrovirus vector for therapeutic use.

A "retroviral vector construct" refers to an assembly which is, within preferred embodiments of the invention, capable of directing the expression of a sequence(s) or gene(s) of interest, such as multivalent binding protein-encoding nucleic acid sequences. Briefly, the retroviral vector construct must include a 5' LTR, a tRNA binding site, a packaging signal, an origin of second strand DNA synthesis and a 3' LTR. A wide variety of heterologous sequences may be included within the vector construct including, for example, sequences which encode a protein (e.g., cytotoxic protein, disease-associated antigen, immune accessory molecule, or replacement gene), or which are useful as a molecule itself (e.g., as a ribozyme or antisense sequence).

Retroviral vector constructs of the present invention may be readily constructed from a wide variety of retroviruses, including for example, B, C, and D type retroviruses as well as spumaviruses and lentiviruses (see, e.g., RNA Tumor Viruses, Second Edition, Cold Spring Harbor Laboratory, 1985). Such retroviruses may be readily obtained from depositories or collections such as the American Type Culture Collection ("ATCC"; Rockville, Maryland), or isolated from known sources using commonly available techniques. Any of the above retroviruses may be readily utilized in order to assemble or construct retroviral vector constructs, packaging cells, or producer cells of the invention, given the disclosure provided herein and standard recombinant techniques (e.g., Sambrook et al, Molecular Cloning: A Laboratory Manual, 2d ed., Cold Spring Harbor Laboratory Press, 1989; Kunkle, 1985 Proc. Natl. Acad. Sci. (USA) 82:488).

Suitable promoters for use in viral vectors generally may include, but are not limited to, the retroviral LTR; the SV40 promoter; and the human cytomegalovirus (CMV) promoter described in Miller, et al., 1989 Biotechniques 7:980-990, or any other promoter (e.g., cellular promoters such as eukaryotic cellular promoters including, but not limited to, the histone, pol III, and p-actin promoters). Other viral promoters that may be employed include, but are not limited to, adenovirus promoters, thymidine kinase (TK) promoters, and B19 parvovirus promoters. The selection of a suitable promoter will be apparent to those skilled in the art from the teachings contained herein, and may be from among either regulated promoters or promoters as described above.

The retroviral plasmid vector is employed to transduce packaging cell lines to form producer cell lines. Examples of packaging cells which may be transfected include, but are not limited to, the PE501, PA317, T-2, y-AM, PA12, T19-14X, VT 19-17-H2, yCRE, CRIP, GP+E-86, GP+envAml2, and DAN cell lines as described in Miller, Human Gene Therapy, 1:5-14 (1990). The vector may transduce the packaging cells through any means known in the art. Such means include, but are not limited to, electroporation, the use of liposomes, and CaPO 4 precipitation. In one alternative, the retroviral plasmid vector may be encapsulated into a liposome, or coupled to a lipid, and then administered to a host.

The producer cell line generates infectious retroviral vector particles which include the nucleic acid sequence(s) encoding the multivalent binding proteins with effector function. Such retroviral vector particles then may be employed to transduce eukaryotic cells, either in vitro or in vivo. The transduced eukaryotic cells will express the nucleic acid sequence(s) encoding the protein or polypeptide. Eukaryotic cells that may be transduced include, but are not limited to, embryonic stem cells, as well as hematopoietic stem cells, hepatocytes, fibroblasts, circulating peripheral blood mononuclear and polymorphonuclear cells including myelomonocytic cells, lymphocytes, myoblasts, tissue macrophages, dendritic cells, Kupffer cells, lymphoid and reticuloendothelial cells of the lymph nodes and spleen, keratinocytes, endothelial cells, and bronchial epithelial cells.

Host cells

A further aspect of the invention provides a host cell transformed or transfected with, or otherwise containing, any of the polynucleotides or cloning/expression constructs of the invention. The polynucleotides and cloning/expression constructs are introduced into suitable cells using any method known in the art, including transformation, transfection and transduction. Host cells include the cells of a subject undergoing ex vivo cell therapy including, for example, ex vivo gene therapy. Eukaryotic host cells contemplated as an aspect of the invention when harboring a polynucleotide, vector, or protein according to the invention include, in addition to a subject's own cells (e.g., a human patient's own cells), VERO cells, HeLa cells, Chinese hamster ovary (CHO) cell lines (including modified CHO cells capable of modifying the glycosylation pattern of expressed multivalent binding molecules, see Published US Patent Application No. 2003/0115614 Al), incorporated herein by reference, COS cells (such as COS-7), W138, BHK, HepG2,3T3, RIN, MDCK, A549, PC12, K562, HEK293 cells, HepG2 cells, N cells, 3T3 cells, Spodopterafrugiperdacells (e.g., Sf9 cells), Saccharomyces cerevisiae cells, and any other eukaryotic cell known in the art to be useful in expressing, and optionally isolating, a protein or peptide according to the invention. Also contemplated are prokaryotic cells, including but not limited to, Escherichiacoli, Bacillus subtilis,

Salmonella typhimurium, a Streptomycete, or any prokaryotic cell known in the art to be suitable for expressing, and optionally isolating, a protein or peptide according to the invention. In isolating protein or peptide from prokaryotic cells, in particular, it is contemplated that techniques known in the art for extracting protein from inclusion bodies may be used. The selection of an appropriate host is within the scope of those skilled in the art from the teachings herein.

The engineered host cells can be cultured in a conventional nutrient medium modified as appropriate for activating promoters, selecting transformants, or amplifying particular genes. The culture conditions for particular host cells selected for expression, such as temperature, pH and the like, will be readily apparent to the ordinarily skilled artisan. Various mammalian cell culture systems can also be employed to express recombinant protein. Examples of mammalian expression systems include the COS-7 lines of monkey kidney fibroblasts, described by Gluzman, 1981 Cell 23:175, and other cell lines capable of expressing a compatible vector, for example, the C127, 3T3, CHO, HeLa and BHK cell lines. Mammalian expression vectors will comprise an origin of replication, a suitable promoter and, optionally, enhancer, and also any necessary ribosome binding sites, polyadenylation site, splice donor and acceptor sites, transcriptional termination sequences, and 5' flanking nontranscribed sequences, for example as described herein regarding the preparation of multivalent binding protein expression constructs. DNA sequences derived from the SV40 splice, and polyadenylation sites may be used to provide the required nontranscribed genetic elements. Introduction of the construct into the host cell can be effected by a variety of methods with which those skilled in the art will be familiar, including but not limited to, calcium phosphate transfection, DEAE-Dextran mediated transfection, or electroporation (Davis et al., 1986 Basic Methods in MolecularBiology).

In one embodiment, a host cell is transduced by a recombinant viral construct directing the expression of a protein or polypeptide according to the invention. The transduced host cell produces viral particles containing expressed protein or polypeptide derived from portions of a host cell membrane incorporated by the viral particles during viral budding.

Pharmaceutical compositions

In some embodiments, the compositions of the invention, such as a multivalent binding protein or a composition comprising a polynucleotide encoding such a protein as described herein, are suitable to be administered under conditions and for a time sufficient to permit expression of the encoded protein in a host cell in vivo or in vitro, for gene therapy, and the like. Such compositions may be formulated into pharmaceutical compositions for administration according to well known methodologies. Pharmaceutical compositions generally comprise one or more recombinant expression constructs, and/or expression products of such constructs, in combination with a pharmaceutically acceptable carrier, excipient or diluent. Such carriers will be nontoxic to recipients at the dosages and concentrations employed. For nucleic acid-based formulations, or for formulations comprising expression products according to the invention, about 0.01 g/kg to about 100 mg/kg body weight will be administered, for example, by the intradermal, subcutaneous, intramuscular or intravenous route, or by any route known in the art to be suitable under a given set of circumstances. A preferred dosage, for example, is about 1 g/kg to about 1 mg/kg, with about 5 g/kg to about 200 g/kg particularly preferred.

It will be evident to those skilled in the art that the number and frequency of administration will be dependent upon the response of the host. Pharmaceutically acceptable carriers for therapeutic use are well known in the pharmaceutical art, and are described, for example, in Remingtons PharmaceuticalSciences, Mack Publishing Co. (A.R. Gennaro edit. 1985). For example, sterile saline and phosphate-buffered saline at physiological pH may be used. Preservatives, stabilizers, dyes and the like may be provided in the pharmaceutical composition. For example, sodium benzoate, sorbic acid and esters ofp-hydroxybenzoic acid may be added as preservatives. Id. at 1449. In addition, antioxidants and suspending agents may be used. Id. The compounds of the present invention may be used in either the free base or salt forms, with both forms being considered as being within the scope of the present invention.

The pharmaceutical compositions that contain one or more nucleic acid constructs of the invention, or the proteins corresponding to the products encoded by such nucleic acid constructs, may be in any form which allows for the composition to be administered to a patient. For example, the composition may be in the form of a solid, liquid or gas (aerosol). Typical routes of administration include, without limitation, oral, topical, parenteral (e.g., sublingually or buccally), sublingual, rectal, vaginal, and intranasal. The term parenteral as used herein includes subcutaneous injections, intravenous, intramuscular, intrasternal, intracavernous, intrathecal, intrameatal, intraurethral injection or infusion techniques. The pharmaceutical composition is formulated so as to allow the active ingredients contained therein to be bioavailable upon administration of the composition to a patient. Compositions that will be administered to a patient take the form of one or more dosage units, where for example, a tablet may be a single dosage unit, and a container of one or more compounds of the invention in aerosol form may hold a plurality of dosage units.

For oral administration, an excipient and/or binder may be present. Examples are sucrose, kaolin, glycerin, starch dextrins, sodium alginate, carboxymethylcellulose and ethyl cellulose. Coloring and/or flavoring agents may be present. A coating shell may be employed.

The composition may be in the form of a liquid, e.g., an elixir, syrup, solution, emulsion or suspension. The liquid may be for oral administration or for delivery by injection, as two examples. When intended for oral administration, preferred compositions contain, in addition to one or more binding domain-immunoglobulin fusion construct or expressed product, one or more of a sweetening agent, preservatives, dye/colorant and flavor enhancer. In a composition intended to be administered by injection, one or more of a surfactant, preservative, wetting agent, dispersing agent, suspending agent, buffer, stabilizer and isotonic agent may be included.

A liquid pharmaceutical composition as used herein, whether in the form of a solution, suspension or other like form, may include one or more of the following adjuvants: sterile diluents such as water for injection, saline solution, preferably physiological saline, Ringer's solution, isotonic sodium chloride, fixed oils such as synthetic mono or digylcerides which may serve as the solvent or suspending medium, polyethylene glycols, glycerin, propylene glycol or other solvents; antibacterial agents such as benzyl alcohol or methyl paraben; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic. Physiological saline is a preferred adjuvant. An injectable pharmaceutical composition is preferably sterile.

It may also be desirable to include other components in the preparation, such as delivery vehicles including, but not limited to, aluminum salts, water-in-oil emulsions, biodegradable oil vehicles, oil-in-water emulsions, biodegradable microcapsules, and liposomes. Examples of immunostimulatory substances (adjuvants) for use in such vehicles include N-acetylmuramyl-L-alanine-D isoglutamine (MDP), lipopolysaccharides (LPS), glucan, IL-12, GM-CSF, gamma interferon and IL-15.

While any suitable carrier known to those of ordinary skill in the art may be employed in the pharmaceutical compositions of this invention, the type of carrier will vary depending on the mode of administration and whether a sustained release is desired. For parenteral administration, such as subcutaneous injection, the carrier preferably comprises water, saline, alcohol, a fat, a wax or a buffer. For oral administration, any of the above carriers or a solid carrier, such as mannitol, lactose, starch, magnesium stearate, sodium saccharine, talcum, cellulose, glucose, sucrose, and magnesium carbonate, may be employed. Biodegradable microspheres (e.g., polylactic galactide) may also be employed as carriers for the pharmaceutical compositions of this invention. Suitable biodegradable microspheres are disclosed, for example, in U.S. Patent Nos. 4,897,268 and 5,075,109. In this regard, it is preferable that the microsphere be larger than approximately 25 microns.

Pharmaceutical compositions may also contain diluents such as buffers, antioxidants such as ascorbic acid, low molecular weight (less than about 10 residues) polypeptides, proteins, amino acids, carbohydrates (e.g., glucose, sucrose or dextrins), chelating agents (e.g., EDTA), glutathione and other stabilizers and excipients. Neutral buffered saline or saline mixed with nonspecific serum albumin are exemplary appropriate diluents. Preferably, product is formulated as alyophilizate using appropriate excipient solutions (e.g., sucrose) as diluents.

The pharmaceutical compositions according to the invention also include stabilized proteins and stable liquid pharmaceutical formulations in accordance with technology known in the art, including the technology disclosed in Published US Patent Application No. 2006/0008415 Al, incorporated herein by reference. Such technologies include derivatization of a protein, wherein the protein comprises a thiol group coupled to N-acetyl-L-cysteine, N-ethyl-maleimide, or cysteine.

As described above, the subject invention includes compositions capable of delivering nucleic acid molecules encoding multivalent binding proteins with effector function. Such compositions include recombinant viral vectors, e.g., retroviruses (see WO 90/07936, WO 91/02805, WO 93/25234, WO 93/25698, and WO 94/03622), adenovirus (see Berkner, 1988 Biotechniques 6:616-627; Li et al., 1993 Hum. Gene Ther. 4:403-409; Vincent et al., Nat, Genet. 5:130-134; and Kolls et al., 1994 Proc. Natl. Acad. Sci. USA 91:215-219), pox virus (see U.S. Patent No. 4,769,330; U.S. Patent No. 5,017,487; and WO 89/01973)), recombinant expression construct nucleic acid molecules complexed to a polycationic molecule (see WO 93/03709), and nucleic acids associated with liposomes (see Wang et al., 1987 Proc. Natl. Acad. Sci. USA 84:7851). In certain embodiments, the DNA may be linked to killed or inactivated adenovirus (see Curielet al., 1992 Hum. Gene Ther. 3:147-154; Cotton et al., 1992 Proc. Natl. Acad. Sci. USA 89:6094). Other suitable compositions include DNA-ligand (see Wu et al., 1989 J. Biol. Chem. 264:16985-16987) and lipid-DNA combinations (see Felgner et al., 1989 Proc. Natl. Acad. Sci. USA 84:7413-7417).

In addition to direct in vivo procedures, ex vivo procedures may be used in which cells are removed from a host (e.g., a subject, such as a human patient), modified, and placed into the same or another host animal. It will be evident that one can utilize any of the compositions noted above for introduction of constructs of the invention, either the proteins/polypeptides or the nucleic acids encoding them into tissue cells in an ex vivo context. Protocols for viral, physical and chemical methods of uptake are well known in the art.

Generation of antibodies

Polyclonal antibodies directed toward an antigen polypeptide generally are produced in animals (e.g., rabbits, hamsters, goats, sheep, horses, pigs, rats, gerbils, guinea pigs, mice, or any other suitable mammal, as well as other non-mammal species) by means of multiple subcutaneous or intraperitoneal injections of antigen polypeptide or a fragment thereof and an adjuvant. Adjuvants include, but are not limited to, complete or incomplete Freund's adjuvant, mineral gels such as aluminum hydroxide, and surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, and dinitrophenol. BCG (bacilli Calmette Guerin) and Corynebacterium parvum are also potentially useful adjuvants. It may be useful to conjugate an antigen polypeptide to a carrier protein that is immunogenic in the species to be immunized; typical carriers include keyhole limpet hemocyanin, serum albumin, bovine thyroglobulin, or soybean trypsin inhibitor. Also, aggregating agents such as alum are used to enhance the immune response. After immunization, the animals are bled and the serum is assayed for anti-antigen polypeptide antibody titer using conventional techniques. Polyclonal antibodies may be utilized in the sera from which they were detected, or may be purified from the sera using, e.g., antigen affinity chromatography.

Monoclonal antibodies directed toward antigen polypeptides are produced using any method which provides for the production of antibody molecules by continuous cell lines in culture. For example, monoclonal antibodies may be made by the hybridoma method as described in Kohler et al., Nature 256:495 [1975]; the human B-cell hybridoma technique (Kosbor et al., Immunol Today 4:72, 1983 ; Cote et al., Proc Natl Acad Sci 80: 2026-2030, 1983) and the EBV-hybridoma technique (Cole et al., Monoclonal Antibodies and Cancer Therapy, Alan R Liss Inc, New York N.Y., pp 77-96, (1985).

When the hybridoma technique is employed, myeloma cell lines may be used. Cell lines suited for use in hybridoma-producing fusion procedures preferably do not produce endogenous antibody, have high fusion efficiency, and exhibit enzyme deficiencies that render them incapable of growing in certain selective media which support the growth of only the desired fused cells (hybridomas). For example, where the immunized animal is a mouse, one may use P3-X63/Ag8, P3-X63-Ag8.653, NS1/1.Ag 4 1, Sp2lO-Ag14, FO, NSO/U, MPC-11, MPCI1-X45-GTG 1.7 and S194/5XXO Bul; for rats, one may use R210.RCY3, Y3-Ag 1.2.3, IR983F and 4B210; and U-266, GM1500-GRG2, LICR-LON-HMy2 and UC729-6 are all useful in connection with cell fusions.

In an alternative embodiment, human antibodies can be produced from phage display libraries (Hoogenboomet al., J. Mol. Biol. 227: 381 [1991]; Marks et al., J. Mol. Biol. 222: 581, see also U.S. Patent No. 5,885,793). ). These processes mimic immune selection through the display of antibody repertoires on the surface of filamentous bacteriophage, and subsequent selection of phage by their binding to an antigen of choice. One such technique is described in PCT Application No. PCT/US98/17364, filed in the name of Adams et al., which describes the isolation of high affinity and functional agonistic antibodies for MPL- and msk-receptors using such an approach. In this approach, a complete repertoire of human antibody genes can be created by cloning naturally rearranged human V genes from peripheral blood lymphocytes as previously described (Mullinax, et al., Proc. Natl. Acad. Sci.(USA) 87: 8095-8099 [1990]).

Alternatively, an entirely synthetic human heavy chain repertoire can be created from unrearranged V gene segments by assembling each human VH segment with D segments of random nucleotides together with a human J segment (Hoogenboom, et al., J. Mol. Biol. 227:381-388 [1992]). Likewise, a light chain repertoire can be constructed by combining each human V segment with a J segment (Griffiths, et al, EMBO J. 13:3245-3260 [1994]). Nucleotides encoding the complete antibody (i.e., both heavy and light chains) are linked as a single-chain Fv fragment and this polynucleotide is ligated to a nucleotide encoding a filamentous phage minor coat protein. When this fusion protein is expressed on the surface of the phage, a polynucleotide encoding a specific antibody can be identified by selection using an immobilized antigen.

Beyond the classic methods of generating polyclonal and monoclonal antibodies, any method for generating any known antibody form is contemplated. In addition to polyclonals and monoclonals, antibody forms include chimerized antibodies, humanized antibodies, CDR-grafted antibodies, and antibody fragments and variants.

Variants and Derivatives of Specific Binding Agents

In one example, insertion variants are provided wherein one or more amino acid residues supplement a specific binding agent amino acid sequence. Insertions may be located at either or both termini of the protein, or may be positioned within internal regions of the specific binding agent amino acid sequence. Variant products of the invention also include mature specific binding agent products, i.e., specific binding agent products wherein leader or signal sequences are removed, and the resulting protein having additional amino terminal residues. The additional amino terminal residues may be derived from another protein, or may include one or more residues that are not identifiable as being derived from a specific protein. Polypeptides with an additional methionine residue at position -l (e.g., Met-I multivalent binding peptides with effector function) are contemplated, as are polypeptides of the invention with additional methionine and lysine residues at positions -2 and -1 (Met-2-Lys--multivalent binding proteins with effector function). Variants of the polypeptides of the invention having additional Met, Met-Lys, or Lys residues (or one or more basic residues in general) are particularly useful for enhanced recombinant protein production in bacterial host cells.

The invention also embraces specific polypeptides of the invention having additional amino acid residues which arise from use of specific expression systems. For example, use of commercially available vectors that express a desired polypeptide as part of a glutathione-S-transferase (GST) fusion product provides the desired polypeptide having an additional glycine residue at position -1 after cleavage of the GST component from the desired polypeptide. Variants which result from expression in other vector systems are also contemplated, including those wherein histidine tags are incorporated into the amino acid sequence, generally at the carboxy and/or amino terminus of the sequence.

In another aspect, the invention provides deletion variants wherein one or more amino acid residues in a polypeptide of the invention are removed. Deletions can be effected at one or both termini of the polypeptide, or from removal of one or more residues within the amino acid sequence. Deletion variants necessarily include all fragments of a polypeptide according to the invention.

Antibody fragments refer to polypeptides having a sequence corresponding to at least part of an immunoglobulin variable region sequence. Fragments may be generated, for example, by enzymatic or chemical cleavage of polypeptides corresponding to full-length antibodies. Other binding fragments include those generated by synthetic techniques or by recombinant DNA techniques, such as the expression of recombinant plasmids containing nucleic acid sequences encoding partial antibody variable regions. Preferred polypeptide fragments display immunological properties unique to, or specific for, a target as described herein. Fragments of the invention having the desired immunological properties can be prepared by any of the methods well known and routinely practiced in the art.

In still another aspect, the invention provides substitution variants of multivalent binding polypeptides having effector function. Substitution variants include those polypeptides wherein one or more amino acid residues in an amino acid sequence are removed and replaced with alternative residues. In some embodiments, the substitutions are conservative in nature; however, the invention embraces substitutions that ore also non-conservative. Amino acids can be classified according to physical properties and contribution to secondary and tertiary protein structure. A conservative substitution is recognized in the art as a substitution of one amino acid for another amino acid that has similar properties. Exemplary conservative substitutions are set out in Table A (see WO 97/09433, page 10, published March 13, 1997 (PCT/GB96/02197, filed 9/6/96), immediately below.

Table A

Conservative Substitutions I SIDE CHAIN CHARACTERISTIC AMINO ACID Aliphatic Non-polar GAPILV Polar -uncharged STMNQ Polar - charged DEKR Aromatic HFWY Other _NQDE

Alternatively, conservative amino acids can be grouped as described in Lehninger, [Biochemistry, Second Edition; Worth Publishers, Inc. NY:NY (1975), pp.71-77] as set out in Table B, immediately below.

Table B

Conservative Substitutions II SIDE CHAIN CHARACTERISTIC AMINO ACID Non-polar A. Aliphatic: ALIVP (hydrophobic) B. Aromatic FW C. Sulfur-containing M D. Borderline G

Uncharged-polar A. Hydroxyl STY B. Amides NQ C. Sulfhydryl C D. Borderline G Positively Charged KRH (Basic) Negatively DE Charged (Acidic)

Conservative Substitutions II

SIDE CHAIN CHARACTERISTIC AMINO ACID

Non-polar (hydrophobic)

A. Aliphatic: ALIVP

B. Aromatic: FW

C. Sulfur-containing: M

D. Borderline: G

Uncharged-polar

A. Hydroxyl: STY

$ B. Amides: NQ

C. Sulfhydryl: C

D, Borderline: G

Positively Charged (Basic) KRH

Negatively Charged (Acidic) DE

The invention also provides derivatives of specific binding agent polypeptides. Derivatives include specific binding agent polypeptides bearing modifications other than insertion, deletion, or substitution of amino acid residues. Preferably, the modifications are covalent in nature, and include for example, chemical bonding with polymers, lipids, other organic, and inorganic moieties. Derivatives of the invention may be prepared to increase circulating half-life of a specific binding agent polypeptide, or may be designed to improve targeting capacity for the polypeptide to desired cells, tissues, or organs.

The invention further embraces multivalent binding proteins with effector function that are covalently modified or derivatized to include one or more water soluble polymer attachments such as polyethylene glycol, polyoxyethylene glycol, or polypropylene glycol, as described U.S. Patent Nos: 4,640,835, 4,496,689, 4,301,144, 4,670,417, 4,791,192 and 4,179,337. Still other useful polymers known in the art include monomethoxy-polyethylene glycol, dextran, cellulose, and other carbohydrate-based polymers, poly-(N-vinyl pyrrolidone)-polyethylene glycol, propylene glycol homopolymers, a polypropylene oxide/ethylene oxide co-polymer, polyoxyethylated polyols (e.g., glycerol) and polyvinyl alcohol, as well as mixtures of these polymers. Particularly preferred are polyethylene glycol (PEG) -derivatized proteins. Water-soluble polymers may be bonded at specific positions, for example at the amino terminus of the proteins and polypeptides according to the invention, or randomly attached to one or more side chains of the polypeptide. The use of PEG for improving therapeutic capacities is described in US Pat. No. 6, 133, 426 to Gonzales, et aL.

Target Sites for Immunoglobulin Mutagenesis

Certain strategies are available to manipulate inherent properties of an antigen specific immunoglobulin (e.g., an antibody) that are not available to non immunoglobulin-based binding molecules. A good example of the strategies favoring, e.g., antibody-based molecules, over these alternatives is the in vivo modulation of the affinity of an antibody for its target through affinity maturation, which takes advantage of the somatic hypermutation of immunoglobulin genes to yield antibodies of increasing affinity as an immune response progresses. Additionally, recombinant technologies have been developed to alter the structure of immunoglobulins and immunoglobulin regions and domains. Thus, polypeptides derived from antibodies may be produced that exhibit altered affinity for a given antigen, and a number of purification protocols and monitoring screens are known in the art for identifying and purifying or isolating these polypeptides. Using these known techniques, polypeptides comprising antibody-derived binding domains can be obtained that exhibit decreased or increased affinity for an antigen. Strategies for generating the polypeptide variants exhibiting altered affinity include the use of site specific or random mutagenesis of the DNA encoding the antibody to change the amino acids present in the protein, followed by a screening step designed to recover antibody variants that exhibit the desired change, e.g., increased or decreased affinity relative to the unmodified parent or referent antibody.

The amino acid residues most commonly targeted in mutagenic strategies to alter affinity are those in the complementarity-determining region (CDR) or hyper variable region of the light and the heavy chain variable regions of an antibody. These regions contain the residues that physicochemically interact with an antigen, as well as other amino acids that affect the spatial arrangement of these residues. However, amino acids in the framework regions of the variable domains outside the

CDR regions have also been shown to make substantial contributions to the antigen binding properties of an antibody, and can be targeted to manipulate such properties. See Hudson, P.J. Curr. Opin. Biotech., 9: 395-402 (1999) and references therein.

Smaller and more effectively screened libraries of antibody variants can be produced by restricting random or site-directed mutagenesis to sites in the CDRs that correspond to areas prone to "hyper-mutation" during the somatic affinity maturation process. See Chowdhury, et al., Nature Biotech., 17: 568-572 (1999) and references therein. The types of DNA elements known to define hyper-mutation sites in this manner include direct and inverted repeats, certain consensus sequences, secondary structures, and palindromes. The consensus DNA sequences include the tetrabase sequence Purine-G-Pyrimidine-A/T (i.e., A or G - G - C or T - A or T) and the serine codon AGY (wherein Y can be C or T).

Thus, another aspect of the invention is a set of mutagenic strategies for modifying the affinity of an antibody for its target. These strategies include mutagenesis of the entire variable region of a heavy and/or light chain, mutagenesis of the CDR regions only, mutagenesis of the consensus hypermutation sites within the CDRs, mutagenesis of framework regions, or any combination of these approaches ("mutagenesis" in this context could be random or site-directed). Definitive delineation of the CDR regions and identification of residues comprising the binding site of an antibody can be accomplished though solving the structure of the antibody in question, and the antibody:ligand complex, through techniques known to those skilled in the art, such as X-ray crystallography, Various methods based on analysis and characterization of such antibody crystal structures are known to those of skill in the art and can be employed to approximate the CDR regions. Examples of such commonly used methods include the Kabat, Chothia, AbM and contact definitions.

The Kabat definition is based on sequence variability and is the most commonly used definition to predict CDR regions. Johnson, et al., Nucleic Acids Research, 28: 214-8 (2000). The Chothia definition is based on the location of the structural loop regions. (Chothia et al., J. Mol. Biol., 196: 901-17 [1986]; Chothia et al., Nature, 342: 877-83 [1989].) The AbM definition is a compromise between the Kabat and Chothia definitions. AbM is an integral suite of programs for antibody structure modeling produced by the Oxford Molecular Group (Martin, et al., Proc.

Nati. Acad. Sci (USA) 86:9268-9272 [1989]; Rees, et al., ABMTM, a computer program for modeling variable regions of antibodies, Oxford, UK; Oxford Molecular, Ltd.). The AbM suite models the tertiary structure of an antibody from primary sequence using a combination of knowledge databases and ab initio methods An additional definition, known as the contact definition, has been recently introduced. See MacCallum et al., J. Mol. Biol., 5:732-45 (1996). This definition is based on an analysis of the available complex crystal structures.

By convention, the CDR domains in the heavy chain are typically referred to as H1, H2 and H3, and are numbered sequentially in order moving from the amino terminus to the carboxy terminus. The CDR regions in the light chain are typically referred to as L1, L2 and L3, and are numbered sequentially in order moving from the amino terminus to the carboxy terminus.

The CDR-Hi is approximately 10 to 12 residues in length and typically starts 4 residues after a Cys according to the Chothia and AbM definitions, or typically 5 residues later according to the Kabat definition. The H I is typically followed by a Trp, typically Trp-Val, but also Trp-Ile, or Trp-Ala. The length of H 1 is approximately 10 to 12 residues according to the AbM definition, while the Chothia definition excludes the last 4 residues.

The CDR-H2 typically starts 15 residues after the end of HI according to the Kabat and AbM definitions. The residues preceding H2 are typically Leu-Glu-Trp Ile-Gly but there are a number of variations. H2 is typically followed by the amino acid sequence Lys/Arg-Leu/Ile/Val/Phe/Thr/Ala-Thr/Ser/Ile/Ala. According to the Kabat definition, the length of H2 is approximately 16 to 19 residues, where the AbM definition predicts the length to be typically 9 to 12 residues.

The CDR-H3 typically starts 33 residues after the end of H2 and is typically preceded by the amino acid sequence Cys-Ala-Arg. H3 is typically followed by the amino acid Gly. The length of H3 ranges from 3 to 25 residues

The CDR-L1 typically starts at approximately residue 24 and will typically follow a Cys. The residue after the CDR-L Iis always Trp and will typically begin one of the following sequences: Trp-Tyr-Gln, Trp-Leu-Gln, Trp-Phe-Gln, or Trp-Tyr Leu. The length of CDR-L1 is approximately 10 to 17 residues.

The CDR-L2 starts approximately 16 residues after the end of L1. It will generally follow residues Ile-Tyr, Val-Tyr, Ile-Lys or Ile-Phe. The length of CDR-L2 is approximately 7 residues.

The CDR-L3 typically starts 33 residues after the end of L2 and typically follows a Cys. L3 is typically followed by the amino acid sequence Phe-Gly-XXX Gly. The length ofL3 is approximately 7 to 11 residues.

Various methods for modifying antibodies have been described in the art, including, e.g., methods of producing humanized antibodies wherein the sequence of the humanized immunoglobulin heavy chain variable region framework is 65% to 95% identical to the sequence of the donor immunoglobulin heavy chain variable region framework. Each humanized immunoglobulin chain will usually comprise, in addition to the CDRs, amino acids from the donor immunoglobulin framework that are, e.g., capable of interacting with the CDRs to effect binding affinity, such as one or more amino acids that are immediately adjacent to a CDR in the donor immunoglobulin or those within about 3 angstroms, as predicted by molecular modeling. The heavy and light chains may each be designed by using any one or all of various position criteria. When combined into an intact antibody, humanized immunoglobuins are substantially non-immunogenic in humans and retain substantially the same affinity as the donor immunoglobulin to the antigen, such as a protein or other compound containing an epitope.

In one example, methods for the production of antibodies, and antibody fragments, are described that have binding specificity similar to a parent antibody, but which have increased human characteristics. Humanized antibodies are obtained by chain shuffling using, for example, phage display technology and a polypeptide comprising the heavy or light chain variable region of a non-human antibody specific for an antigen of interest, which is then combined with a repertoire of human complementary (light or heavy) chain variable regions. Hybrid pairings which are specific for the antigen of interest are identified and human chains from the selected pairings are combined with a repertoire of human complementary variable domains (heavy or light). In another embodiment, a component of a CDR from a non-human antibody is combined with a repertoire of component parts of CDRs from human antibodies. From the resulting library of antibody polypeptide dimers, hybrids are selected and may used in a second humanizing shuffling step; alternatively, this second step is eliminated if the hybrid is already of sufficient human character to be of therapeutic value. Methods of modification to increase human character are known in the art.

Another example is a method for making humanized antibodies by substituting a CDR amino acid sequence for the corresponding human CDR amino acid sequence and/or substituting a FR amino acid sequence for the corresponding human FR amino acid sequences.

Yet another example provides methods for identifying the amino acid residues of an antibody variable domain that may be modified without diminishing the native affinity of the antigen binding domain while reducing its immunogenicity with respect to a heterologous species and methods for preparing these modified antibody variable regions as useful for administration to heterologous species.

Modification of an immunoglobulin such as an antibody by any of the methods known in the art is designed to achieve increased or decreased binding affinity for an antigen and/or to reduce immunogenicity of the antibody in the recipient and/or to modulate effector activity levels. In one approach, humanized antibodies can be modified to eliminate glycosylation sites in order to increase affinity of the antibody for its cognate antigen (Co, et al., Mol. Immunol. 30:1361-1367

[1993]). Techniques such as "reshaping," hyperchimerization," and "veneering/resurfacing" have produced humanized antibodies with greater therapeutic potential. Vaswami, et al., Annals of Allergy, Asthma, & Immunol 81:105 (1998); Roguska, et al., Prot. Engineer. 9:895-904 (1996)]. See also US Pat. No. 6,072,035, which describes methods for reshaping antibodies. While these techniques diminish antibody immunogenicity by reducing the number of foreign residues, they do not prevent anti-idiotypic and anti-allotypic responses following repeated administration of the antibodies. Alternatives to these methods for reducing immunogenicity are described in Gilliland et al., J. Immunol. 62(6):3663-71 (1999).

In many instances, humanizing antibodies results in a loss of antigen binding capacity. It is therefore preferable to "back mutate" the humanized antibody to include one or more of the amino acid residues found in the original (most often rodent) antibody in an attempt to restore binding affinity of the antibody. See, for example, Saldanha et al., Mol. Immunol. 36:709-19 (1999).

Glycosylation of immunoglobulins has been shown to affect effector functions, structural stability, and the rate of secretion from antibody-producing cells (see Leatherbarrow et al., Mol. Immunol. 22:407 (1985), incorporated herein by reference). The carbohydrate groups responsible for these properties are generally attached to the constant regions of antibodies. For example, glycosylation of IgG at Asn 297 in the CH2 domain facilitates full capacity of the IgG to activate complement dependent cytolysis (Tao et al., J. Immunol. 143:2595 (1989)). Glycosylation of IgM at Asn 402 in the CH3 domain, for example, facilitates proper assembly and cytolytic activity of the antibody (Muraoka et al., J. Immunol. 142:695 (1989)). Removal of glycosylation sites at positions 162 and 419 in the CHI and CH3 domains of an IgA antibody led to intracellular degradation and at least 90% inhibition of secretion (Taylor et al., Wall, Mol. Cell. Biol. 8:4197 (1988)). Accordingly, the molecules of the invention include mutationally altered immunoglobulins exhibiting altered glycosylation patterns by mutation of specific residues in, e.g., a constant sub-region to alter effector function. See Co et al., Mol. Immunol. 30:1361-1367 (1993), Jacquemon et al., J. Thromb. Haemost. 4:1047-1055 (2006), Schuster et al., Cancer Res. 65:7934-7941 (2005), and Warnock et al., Biotechnol Bioeng. 92:831-842 (2005), each incorporated herein by reference.

The invention also includes multivalent binding molecules having at least one binding domain that is at least 80%, preferably 90% or 95% or 99% identical in sequence to a known immunoglobulin variable region sequence and which has at least one residue that differs from such immunoglobulin variable region, wherein the changed residue adds a glycosylation site, changes the location of one or more glycosylation site(s), or preferably removes a glycosylation site relative to the immunoglobulin variable region. In some embodiments, the change removes an N linked glycosylation site in a an immunoglobulin variable region framework, or removes an N-linked glycosylation site that occurs in the immunoglobulin heavy chain variable region framework in the region spanning about amino acid residue 65 to about amino acid residue 85, using the numbering convention of Co et al., J. Immunol. 148: 1149, (1992).

Any method known in the art is contemplated for producing the multivalent binding molecules exhibiting altered glycosylation patterns relative to an immunoglobulin referent sequence. For example, any of a variety of genetic techniques may be employed to alter one or more particular residues. Alternatively, the host cells used for production may be engineered to produce the altered glycosylation pattern. One method known in the art, for example, provides altered glycosylation in the form of bisected, non-fucosylated variants that increase ADCC. The variants result from expression in a host cell containing an oligosaccharide modifying enzyme. Alternatively, the Potelligent technology of BioWa/Kyowa Hakko is contemplated to reduce the fucose content of glycosylated molecules according to the invention. In one known method, a CHO host cell for recombinant immunoglobulin production is provided that modifies the glycosylation pattern of the immunoglobulin Fc region, through production of GDP-fucose. This technology is available to modify the glycosylation pattern of a constant sub-region of a multivalent binding molecule according to the invention.

In addition to modifying the binding properties of binding domains, such as the binding domains of immunoglobulins, and in addition to such modifications as humanization, the invention comprehends the modulation of effector function by changing or mutating residues contributing to effector function, such as the effector function of a constant sub-region. These modifications can be effected using any technique known in the art, such as the approach disclosed in Presta et al., Biochem. Soc. Trans. 30:487-490 (2001), incorporated herein by reference. Exemplary approaches would include the use of the protocol disclosed in Presta et al. to modify specific residues known to affect binding in one or more constant sub-regions corresponding to FCyRI, FCyRI, FCyRIII, FCaR, and FCER.

In another approach, the Xencor XmAb technology is available to engineer constant sub-regions corresponding to Fe domains to enhance cell killing effector function. See Lazar et al., Proc. Natl. Acad. Sci. (USA) 103(11):4005-4010 (2006), incorporated herein by reference. Using this approach, for example, one can generate constant sub-regions optimized for FcyR specificity and binding, thereby enhancing cell killing effector function.

Production of multivalent binding proteins with effector function

A variety of expression vector/host systems may be utilized to contain and express the multivalent binding protein (with effector function) of the invention. These systems include but are not limited to microorganisms such as bacteria transformed with recombinant bacteriophage, plasmid, cosmid, or other expression vectors; yeast transformed with yeast expression or shuttle vectors; insect cell systems infected with virus expression vectors (e.g., baculovirus); plant cell systems transfected with virus expression vectors (e.g., cauliflower mosaic virus, CaMV; tobacco mosaic virus, TMV) or transformed with bacterial expression vectors (e.g., Ti or pBR322 plasmid); or animal cell systems. Mammalian cells that are useful in recombinant multivalent binding protein productions include, but are not limited to, VERO cells, HeLa cells, Chinese hamster ovary (CHO) cell lines, COS cells (such as COS-7), Wi38, BHK, HepG2, 3T3, RIN, MDCK, A549, PCl2, K562 and HEK293 cells. Exemplary protocols for the recombinant expression of the multivalent binding protein are described herein below.

An expression vector can comprise a transcriptional unit comprising an assembly of (1) a genetic element or elements having a regulatory role in gene expression, for example, a promoter, enhancer, or factor-specific binding site, (2) a structural or sequence that encodes the binding agent which is transcribed into mRNA and translated into protein, and (3) appropriate transcription initiation and termination sequences. Structural units intended for use in yeast or eukaryotic expression systems preferably include a leader sequence enabling extracellular secretion of translated protein by a host cell. Alternatively, where recombinant multivalent binding protein is expressed without a leader or transport sequence, it may include an amino terminal methionine residue. This residue may or may not be subsequently cleaved from the expressed recombinant protein to provide a final multivalent binding protein.

For example, the multivalent binding proteins may be recombinantly expressed in yeast using a commercially available expression system, e.g., the Pichia Expression System (Invitrogen, San Diego, CA), following the manufacturer's instructions. This system also relies on the pre-pro-alpha sequence to direct secretion, but transcription of the insert is driven by the alcohol oxidase (AOX1) promoter upon induction by methanol. The secreted multivalent binding peptide may be purified from the yeast growth medium by, e.g., the methods used to purify the peptide from bacterial and mammalian cell supernatants.

Alternatively, the cDNA encoding the multivalent binding peptide may be cloned into the baculovirus expression vector pVL1393 (PharMingen, San Diego, CA). This vector can be used according to the manufacturer's directions (PharMingen) to infect Spodoptera frugiperda cells in SF9 protein-free medium and to produce recombinant protein. The multivalent binding protein can be purified and concentrated from the medium using a heparin-Sepharose column (Pharmacia, Piscataway, NJ). Insect systems for protein expression, such as the SF9 system, are well known to those of skill in the art. In one such system, Autographa californica nuclear polyhedrosis virus (AcNPV) can be used as a vector to express foreign genes in the Spodopterafrugiperdacells or in Trichoplusia larvae. The multivalent binding peptide coding sequence can be cloned into a nonessential region of the virus, such as the polyhedrin gene, and placed under control of the polyhedrin promoter. Successful insertion of the multivalent binding peptide will render the polyhedrin gene inactive and produce recombinant virus lacking coat protein. The recombinant viruses can be used to infect S. frugiperda cells or Trichoplusia larvae in which peptide is expressed (Smith et al., J Virol 46: 584, 1983; Engelhard et al., Proc Nat Acad Sci (USA) 91: 3224-7, 1994).

In another example, the DNA sequence encoding the multivalent binding peptide can be amplified by PCR and cloned into an appropriate vector, for example, pGEX-3X (Pharmacia, Piscataway, NJ). The pGEX vector is designed to produce a fusion protein comprising glutathione-S-transferase (GST), encoded by the vector, and a multivalent binding protein encoded by a DNA fragment inserted into the cloning site of the vector. The primers for the PCR can be generated to include for example, an appropriate cleavage site. Where the multivalent binding protein fusion moiety is used solely to facilitate expression or is otherwise not desirable as an attachment to the peptide of interest, the recombinant multivalent binding protein fusion may then be cleaved from the GST portion of the fusion protein. The pGEX 3X/multivalent binding peptide construct is transformed into E. coliXL-1 Blue cells (Stratagene, La Jolla CA), and individual transformants isolated and grown. Plasmid DNA from individual transformants is purified and may be partially sequenced using an automated sequencer to confirm the presence of the desired multivalent binding protein-encoding nucleic acid insert in the proper orientation.

The fused multivalent binding protein, which may be produced as an insoluble inclusion body in the bacteria, can be purified as follows. Host cells can be harvested by centrifugation; washed in 0.15 M NaCl, 10 mM Tris, pH 8, 1 mM EDTA; and treated with 0.1 mg/ml lysozyme (Sigma Chemical Co.) for 15 minutes at room temperature. The lysate can be cleared by sonication, and cell debris can be pelleted by centrifugation for 10 minutes at 12,000 X g. The multivalent binding protein fusion-containing pellet can be resuspended in 50 mM Tris, pH 8, and 10 mM EDTA, layered over 50% glycerol, and centrifuged for 30 minutes at 6000g. The pellet can be resuspended in standard phosphate buffered saline solution (PBS) free of Mg+* and Ca+*. The multivalent binding protein fusion can be further purified by fractionating the resuspended pellet in a denaturing SDS polyacrylamide gel (Sambrook et aL). The gel is soaked in 0.4 M KCI to visualize the protein, which is excised and electroeluted in gel-running buffer lacking SDS. If the GST/multivalent binding peptide fusion protein is produced in bacteria as a soluble protein, it can be purified using the GST Purification Module (Pharmacia Biotech).

The multivalent binding protein fusion is preferably subjected to digestion to cleave the GST from the multivalent binding peptide of the invention. The digestion reaction (20-40 pg fusion protein, 20-30 units human thrombin (4000 U/mg (Sigma) in 0.5 ml PBS) can be incubated 16-48 hours at room temperature and loaded on a denaturing SDS-PAGE gel to fractionate the reaction products. The gel can be soaked in 0.4 M KCl to visualize the protein bands. The identity of the protein band corresponding to the expected molecular weight of the multivalent binding peptide can be confirmed by amino acid sequence analysis using an automated sequencer (Applied Biosystems Model 473A, Foster City, CA). Alternatively, the identity can be confirmed by performing HPLC and/or mass spectrometry of the peptides.

Alternatively, a DNA sequence encoding the multivalent binding peptide can be cloned into a plasmid containing a desired promoter and, optionally, a leader sequence (see, e.g., Better et al., Science, 240:1041-43, 1988). The sequence of this construct can be confirmed by automated sequencing. The plasmid can then be transformed into a suitable E. coli strain, such as strain MCI061, using standard procedures employing CaCl2 incubation and heat shock treatment of the bacteria (Sambrook et al.). The transformed bacteria can be grown in LB medium supplemented with carbenicillin or another suitable form of selection as would be known in the art, and production of the expressed protein can be induced by growth in a suitable medium. If present, the leader sequence can effect secretion of the multivalent binding peptide and be cleaved during secretion. The secreted recombinant protein can be purified from the bacterial culture medium by the methods described herein below.

Mammalian host systems for the expression of the recombinant protein are well known to those of skill in the art and are preferred systems. Host cell strains can be chosen for a particular ability to process the expressed protein or produce certain post-translation modifications that will be useful in providing protein activity. Such modifications of the polypeptide include, but are not limited to, acetylation, carboxylation, glycosylation, phosphorylation, lipidation and acylation. Different host cells such as CHO, HeLa, MDCK, 293, W138, and the like, have specific cellular machinery and characteristic mechanisms for such post-translational activities and can be chosen to ensure the correct modification and processing of the foreign protein.

It is preferable that the transformed cells be used for long-term, high-yield protein production and, as such, stable expression is desirable. Once such cells are transformed with vectors that preferably contain at least one selectable marker along with the desired expression cassette, the cells are grown for 1-2 days in an enriched medium before being switched to selective medium. The selectable marker is designed to confer resistance to selection and its presence allows growth and recovery of cells that successfully express the foreign protein. Resistant clumps of stably transformed cells can be proliferated using tissue culture techniques appropriate to the cell.

A number of selection systems can be used to recover the cells that have been transformed for recombinant protein production. Such selection systems include, but are not limited to, HSV thymidine kinase, hypoxanthine-guanine phosphoribosyltransferase and adenine phosphoribosyltransferase genes, in tk-, hgprt or aprt- cells, respectively. Also, anti-metabolite resistance can be used as the basis of selection for dhfr, which confers resistance to methotrexate; gpt, which confers resistance to mycophenolic acid; neo, which confers resistance to the aminoglycoside G418 and confers resistance to chlorsulfuron; and hygro, which confers resistance to hygromycin. Additional selectable genes that may be useful include trpB, which allows cells to utilize indole in place of tryptophan, or hisD, which allows cells to utilize histinol in place of histidine. Markers that give a visual indication for identification of transformants include anthocyanins, p-glucuronidase and its substrate, GUS, and luciferase and its substrate, luciferin.

Purification of Proteins

Protein purification techniques are well known to those of skill in the art. These techniques involve, at one level, the crude fractionation of the polypeptide and non-polypeptide fractions. Having separated the multivalent binding polypeptide from at least one other protein, the polypeptide of interest is purified, but further purification using chromatographic and electrophoretic techniques to achieve partial or complete purification (or purification to homogeneity) is frequently desired. Analytical methods particularly suited to the preparation of a pure multivalent binding peptide are ion-exchange chromatography, exclusion chromatography; polyacrylamide gel electrophoresis; and isoelectric focusing. Particularly efficient methods of purifying peptides are fast protein liquid chromatography and HPLC.

Certain aspects of the present invention concern the purification, and in particular embodiments, the substantial purification, of an encoded multivalent binding protein or peptide. The term "purified multivalent binding protein or peptide" as used herein, is intended to refer to a composition, isolatable from other components, wherein the multivalent binding protein or peptide is purified to any degree relative to its naturally obtainable state. A purified multivalent binding protein or peptide therefore also refers to a multivalent binding protein or peptide, free from the environment in which it may naturally occur.

Generally, "purified" will refer to a multivalent binding protein composition that has been subjected to fractionation to remove various other components, and which composition substantially retains its expressed biological activity. Where the term "substantially purified" is used, this designation refers to a multivalent binding protein composition in which the multivalent binding protein or peptide forms the major component of the composition, such as constituting about 50%, about 60%, about 70%, about 80%, about 90%, about 95%, about 99% or more of the protein, by weight, in the composition.

Various methods for quantifying the degree of purification of the multivalent binding protein will be known to those of skill in the art in light of the present disclosure. These include, for example, determining the specific binding activity of an active fraction, or assessing the amount of multivalent binding polypeptides within a fraction by SDS/PAGE analysis. A preferred method for assessing the purity of a multivalent binding protein fraction is to calculate the binding activity of the fraction, to compare it to the binding activity of the initial extract, and to thus calculate the degree of purification, herein assessed by a "-fold purification number." The actual units used to represent the amount of binding activity will, of course, be dependent upon the particular assay technique chosen to follow the purification and whether or not the expressed multivalent binding protein or peptide exhibits a detectable binding activity.

Various techniques suitable for use in multivalent binding protein purification are well known to those of skill in the art. These include, for example, precipitation with ammonium sulfate, PEG, antibodies and the like, or by heat denaturation, followed by centrifugation; chromatography steps such as ion exchange, gel filtration, reverse phase, hydroxylapatite and affinity chromatography; isoelectric focusing; gel electrophoresis; and combinations of these and other techniques. As is generally known in the art, it is believed that the order of conducting the various purification steps may be changed, or that certain steps may be omitted, and still result in a suitable method for the preparation of a substantially purified multivalent binding protein.

There is no general requirement that the multivalent binding protein always be provided in its most purified state. Indeed, it is contemplated that less substantially multivalent binding proteins will have utility in certain embodiments. Partial purification may be accomplished by using fewer purification steps in combination, or by utilizing different forms of the same general purification scheme. For example, it is appreciated that a cation-exchange column chromatography performed utilizing an HPLC apparatus will generally result in greater purification than the same technique utilizing a low pressure chromatography system. Methods exhibiting a lower degree of relative purification may have advantages in total recovery of multivalent binding protein product, or in maintaining binding activity of an expressed multivalent binding protein.

It is known that the migration of a polypeptide can vary, sometimes significantly, with different conditions of SDS/PAGE (Capaldi et al., Biochem. Biophys. Res. Comm., 76:425, 1977). It will therefore be appreciated that under differing electrophoresis conditions, the apparent molecular weights of purified or partially purified multivalent binding protein expression products may vary.

Effector cells

Effector cells for inducing, e.g., ADCC, ADCP (antibody-dependent cellular phagocytosis), and the like, against a target cell include human leukocytes, macrophages, monocytes, activated neutrophils, activated natural killer (NK) cells, and eosinophils. Effector cells express FaR (CD89), FcyRI, FecyRII, FyRIII, and/or FcsRl and include, for example, monocytes and activated neutrophils. Expression of FcyRI, e.g., has been found to be up-regulated by interferon gamma (IFN-y). This enhanced expression increases the cytotoxic activity of monocytes and neutrophils against target cells. Accordingly, effector cells may be activated with (IFN-y) or other cytokines (e.g., TNF-a or , colony stimulating factor, IL-2) to increase the presence of FcyRl on the surface of the cells prior to being contacted with a multivalent protein of the invention.

The multivalent proteins of the invention provide an antibody effector function, such as antibody-dependent effector cell-mediated cytotoxicity (ADCC), for use against a target cell. Multivalent proteins with effector function are administered alone, as taught herein, or after being coupled to an effector cell, thereby forming an "activated effector cell." An "activated effector cell" is an effector cell, as defined herein, linked to a multivalent protein with effector function, also as defined herein, such that the effector cell is effectively provided with a targeting function prior to administration.

Activated effector cells are administered in vivo as a suspension of cells in a physiologically acceptable solution. The number of cells administered is on the order of 10-10', but will vary depending on the therapeutic purpose. In general, the amount wilt be sufficient to obtain localization of the effector cell at the target cell, and to provide a desired level of effector cell function in that locale, such as cell killing by ADCC and/or phagocytosis. The term physiologically acceptable solution, as used herein, is intended to include any carrier solution which stabilizes the targeted effector cells for administration in vivo including, for example, saline and aqueous buffer solutions, solvents, antibacterial and antifungal agents, isotonic agents, and the like.

Accordingly, another aspect of the invention provides a method of inducing a specific antibody effector function, such as ADCC, against a cell in a subject, comprising administering to the subject a multivalent protein (or encoding nucleic acid) or activated effector cell in a physiologically acceptable medium. Routes of administration can vary and suitable administration routes will be determined by those of skill in the art based on a consideration of case-specific variables and routine procedures, as is known in the art.

Cell-free effects

Cell-free effects are also provided by the multivalent molecules of the invention, e.g., by providing a CDC functionality. The complement system is a biochemical cascade of the immune system that helps clear foreign matter such as pathogens from an organism. It is derived from many small plasma proteins that work together in inducing cytolysis of a target cell by disrupting the target cell's plasma membrane. The complement system consists of more than 35 soluble and cell-bound proteins, 12 of which are directly involved in the complement pathways. The proteins are active in three biochemical pathways leading to the activation of the complement system: the classical complement pathway, the alternate complement pathway, and the mannose-binding lectin pathway. Antibodies, in particular the IgG1 class, can also "fix" complement. A detailed understanding of these pathways has been achieved in the art and will not be repeated here, but it is worth noting that complement-dependent cytotoxicity is not dependent on the interaction of a binding molecule with a cell, e.g., a B cell, of the immune system. Also worth noting is that the complement system is regulated by complement regulating proteins. These proteins are present at higher concentrations in the blood plasma than the complement proteins. The complement regulating proteins are found on the surfaces of self-cells, providing a mechanism to prevent self-cells from being targeted oy complement proteins. It is expected that the complement system plays a role in several diseases with an immune component, such as Barraquer-Simons Syndrome, Alzheimer's disease, asthma, lupus erythematosus, various forms of arthritis, autoimmune heart disease, and multiple sclerosis. Deficiencies in the terminal pathway predispose an individual to both autoimmune disease and infections (particularly meningitis).

Diseases, disorders and conditions

The invention provides a multivalent binding proteins with effector function, and variant and derivative thereof, that bind to one or more binding partners and those binding events are useful in the treatment, prevention, or amelioration of a symptom associated with a disease, disorder or pathological condition, preferably one afflicting humans. In preferred embodiments of these methods, the multivalent (and multispecific) binding protein with effector function associates a cell bearing a target, such as a tumor-specific cell-surface marker, with an effector cell, such as a cell of the immune system exhibiting cytotoxic activity. In other embodiments, the multispecific, multivalent binding protein with effector function specifically binds two different disease-, disorder- or condition-specific cell-surface markers to ensure that the correct target is associated with an effector cell, such as a cytotoxic cell of the immune system. Additionally, the multivalent binding protein with effector function can be used to induce or increase antigen activity, or to inhibit antigen activity. The multivalent binding proteins with effector function are also suitable for combination therapies and palliative regimes.

In one aspect, the present invention provides compositions and methods useful for treating or preventing diseases and conditions characterized by aberrant levels of antigen activity associated with a cell. These diseases include cancers and other hyperproliferative conditions, such as hyperplasia, psoriasis, contact dermatitis, immunological disorders, and infertility. A wide variety of cancers, including solid tumors and leukemias are amenable to the compositions and methods disclosed herein. Types of cancer that may be treated include, but are not limited to: adenocarcinoma of the breast, prostate, and colon; all forms of bronchogenic carcinoma of the lung; myeloid; melanoma; hepatoma; neuroblastoma; papilloma; apudoma; choristoma; branchioma; malignant carcinoid syndrome; carcinoid heart disease; and carcinoma (e.g., Walker, basal cell, basosquamous, nrown-rearce, ductal, Ehrlich tumor, Krebs 2, merkel cell, mucinous, non-small cell lung, oat cell, papillary, scirrhous, bronchiolar, bronchogenic, squamous cell, and transitional cell). Additional types of cancers that may be treated include: histiocytic disorders; leukemia; histiocytosis malignant; Hodgkin's disease; immunoproliferative small; non-Hodgkin's lymphoma; plasmacytoma; reticuloendotheliosis; melanoma; chondroblastoma; chondroma; chondrosarcoma; fibroma; fibrosarcoma; giant cell tumors; histiocytoma; lipoma; liposarcoma; mesothelioma; myxoma; myxosarcoma; osteoma; osteosarcoma; chordoma; craniopharyngioma; dysgerminoma; hamartoma; mesenchymoma; mesonephroma; myosarcoma; ameloblastoma; cementoma; odontoma; teratoma; thymoma; trophoblastic tumor. Further, the following types of cancers are also contemplated as amenable to treatment: adenoma; cholangioma; cholesteatoma; cyclindroma; cystadenocarcinoma; cystadenoma; granulosa cell tumor; gynandroblastoma; hepatoma; hidradenoma; islet cell tumor; Leydig cell tumor; papilloma; sertoli cell tumor; theca cell tumor; leimyoma; leiomyosarcoma; myoblastoma; myomma; myosarcoma; rhabdomyoma; rhabdomyosarcoma; ependymoma; ganglioneuroma; glioma; medulloblastoma; meningioma; neurilemmoma; neuroblastoma; neuroepithelioma; neurofibroma; neuroma; paraganglioma; paraganglioma nonchromaffin. The types of cancers that may be treated also include, but are not limited to, angiokeratoma; angiolymphoid hyperplasia with eosinophilia; angioma sclerosing; angiomatosis; glomangioma; hemangioendothelioma; hemangioma; hemangiopericytoma; hemangiosarcoma; lymphangioma; lymphangiomyoma; lymphangiosarcoma; pinealoma; carcinosarcoma; chondrosarcoma; cystosarcoma phyllodes; fibrosarcoma; hemangiosarcoma; leiomyosarcoma; leukosarcoma; liposarcoma; lymphangiosarcoma; myosarcoma; myxosarcoma; ovarian carcinoma; rhabdomyosarcoma; sarcoma; neoplasms; nerofibromatosis; and cervical dysplasia. The invention further provides compositions and methods useful in the treatment of other conditions in which cells have become immortalized or hyperproliferative due to abnormally high expression of antigen.

Exemplifying the variety of hyperproliferative disorders amenable to the compositions and methods of the invention are B-cell cancers, including B-cell lymphomas (such as various forms of Hodgkin's disease, non-Hodgkins lymphoma

(NHL) or central nervous system lymphomas), leukemias (such as acute lymphoblastic leukemia (ALL), chronic lymphocytic leukemia (CLL), Hairy cell leukemia and chronic myoblastic leukemia) and myelomas (such as multiple myeloma). Additional B cell cancers include small lymphocytic lymphoma, B-cell prolymphocytic leukemia, lymphoplasmacytic lymphoma, splenic marginal zone lymphoma, plasma cell myeloma, solitary plasmacytoma of bone, extraosseous plasmacytoma, extra-nodal marginal zone B-cell lymphoma of mucosa-associated (MALT) lymphoid tissue, nodal marginal zone B-cell lymphoma, follicular lymphoma, mantle cell lymphoma, diffuse large B-cell lymphoma, mediastinal (thymic) large B-cell lymphoma, intravascular large B-cell lymphoma, primary effusion lymphoma, Burkitt's lymphoma/leukemia, B-cell proliferations of uncertain malignant potential, lymphomatoid granulomatosis, and post-transplant lymphoproliferative disorder.

Disorders characterized by autoantibody production are often considered autoimmune diseases. Autoimmune diseases include, but are not limited to: arthritis, rheumatoid arthritis, juvenile rheumatoid arthritis, osteoarthritis, polychondritis, psoriatic arthritis, psoriasis, dermatitis, polymyositis/dermatomyositis, inclusion body myositis, inflammatory myositis, toxic epidermal necrolysis, systemic scleroderma and sclerosis, CREST syndrome, responses associated with inflammatory bowel disease, Crohn's disease, ulcerative colitis, respiratory distress syndrome, adult respiratory distress syndrome (ARDS), meningitis, encephalitis, uveitis, colitis, glomerulonephritis, allergic conditions, eczema, asthma, conditions involving infiltration of T cells and chronic inflammatory responses, atherosclerosis, autoimmune myocarditis, leukocyte adhesion deficiency, systemic lupus erythematosus (SLE), subacute cutaneous lupus erythematosus, discoid lupus, lupus myelitis, lupus cerebritis, juvenile onset diabetes, multiple sclerosis, allergic encephalomylitis, neuromyelitis optica, rheumatic fever, Sydenham's chorea, immune responses associated with acute and delayed hypersensitivity mediated by cytokines and T-lymphocytes, tuberculosis, sarcoidosis, granulomatosis including Wegener's granulomatosis and Churg-Strauss disease, agranulocytosis, vasculitis (including hypersensitivity vasculitis/angiitis, ANCA and rheumatoid vasculitis), aplastic anemia, Diamond Blackfan anemia, immune hemolytic anemia including autoimmune hemolytic anemia (AIHA), pernicious anemia, pure red cell aplasia

(PRCA), Factor VIII deficiency, hemophilia A, autoimmune neutropenia, pancytopenia, leukopenia, diseases involving leukocyte diapedesis, central nervous system (CNS) inflammatory disorders, multiple organ injury syndrome, myasthenia gravis, antigen-antibody complex mediated diseases, anti-glomerular basement membrane disease, anti-phospholipid antibody syndrome, allergic neuritis, Behcet disease, Castleman's syndrome, Goodpasture's syndrome, Lambert-Eaton Myasthenic Syndrome, Reynaud's syndrome, Sjorgen's syndrome, Stevens-Johnson syndrome, solid organ transplant rejection, graft versus host disease (GVHD), bullous pemphigoid, pemphigus, autoimmune polyendocrinopathies, seronegative spondyloarthropathies, Reiter's disease, stiff-man syndrome, giant cell arteritis, immune complex nephritis, IgA nephropathy, IgM polyneuropathies or IgM mediated neuropathy, idiopathic thrombocytopenic purpura (ITP), thrombotic throbocytopenic purpura (TTP), Henoch-Schonlein purpura, autoimmune thrombocytopenia, autoimmune disease of the testis and ovary including autoimmune orchitis and oophoritis, primary hypothyroidism; autoimmune endocrine diseases including autoimmune thyroiditis, chronic thyroiditis (Hashimoto's Thyroiditis), subacute thyroiditis, idiopathic hypothyroidism, Addison's disease, Grave's disease, autoimmune polyglandular syndromes (or polyglandular endocrinopathy syndromes), Type I diabetes also referred to as insulin-dependent diabetes mellitus (IDDM) and Sheehan's syndrome; autoimmune hepatitis, lymphoid interstitial pneumonitis (HIV), bronchiolitis obliterans (non-transplant) vs NSIP, Guillain-Barr6 Syndrome, large vessel vasculitis (including polymyalgia rheumatica and giant cell (Takayasu's) arteritis), medium vessel vasculitis (including Kawasaki's disease and polyarteritis nodosa), polyarteritis nodosa (PAN) ankylosing spondylitis, Berger's disease (IgA nephropathy), rapidly progressive glomerulonephritis, primary biliary cirrhosis, Celiac sprue (gluten enteropathy), cryoglobulinemia, cryoglobulinemia associated with hepatitis, amyotrophic lateral sclerosis (ALS), coronary artery disease, familial Mediterranean fever, microscopic polyangiitis, Cogan's syndrome, Whiskott-Aldrich syndrome and thromboangiitis obliterans.

Rheumatoid arthritis (RA) is a chronic disease characterized by inflammation of the joints, leading to swelling, pain, and loss of function. Patients having RA for an extended period usually exhibit progressive joint destruction, deformity, disability and even premature death. Beyond RA, inflammatory diseases, disorders and conditions in general are amenable to treatment, prevention or ameoranon or symptoms (e.g., heat, pain, swelling, redness) associated with the process of inflammation, and the compositions and methods of the invention are beneficial in treating, preventing or ameliorating aberrant or abnormal inflammatory processes, including RA.

Crohn's disease and a related disease, ulcerative colitis, are the two main disease categories that belong to a group of illnesses called inflammatory bowel disease (IBD). Crohn's disease is a chronic disorder that causes inflammation of the digestive or gastrointestinal (GI) tract. Although it can involve any area of the GI tract from the mouth to the anus, it most commonly affects the small intestine and/or colon. In ulcerative colitis, the GI involvement is limited to the colon. Crohn's disease may be characterized by antibodies against neutrophil antigens, i.e., the "perinuclear anti-neutrophil antibody" (pANCA), and Saccharomyces cervisiae, i.e. the "anti-Saccharomyces cerevisiae antibody" (ASCA). Many patients with ulcerative colitis have the pANCA antibody in their blood, but not the ASCA antibody, while many Crohn's patients exhibit ASCA antibodies, and not pANCA antibodies. One method of evaluating Crohn's disease is using the Crohn's disease Activity Index (CDAI), based on 18 predictor variables scores collected by physicians. CDAI values of 150 and below are associated with quiescent disease; values above that indicate active disease, and values above 450 are seen with extremely severe disease [Best et al., "Development of a Crohn's disease activity index." Gastroenterology 70:439-444 (1976)]. However, since the original study, some researchers use a'subjective value' of 200 to 250 as an healthy score.

Systemic Lupus Erythematosus (SLE) is an autoimmune disease caused by recurrent injuries to blood vessels in multiple organs, including the kidney, skin, and joints. In patients with SLE, a faulty interaction between T cells and B-cells results in the production of autoantibodies that attack the cell nucleus. There is general agreement that autoantibodies are responsible for SLE, so new therapies that deplete the B-cell lineage, allowing the immune system to reset as new B-cells are generated from precursors, would offer hope for long lasting benefit in SLE patients.

Multiple sclerosis (MS) is also an autoimmune disease. It is characterized by inflammation of the central nervous system and destruction of myelin, which insulates nerve cell fibers in the brain, spinal cord, and body. Although the cause o ivi is unknown, it is widely believed that autoimmune T cells are primary contributors to the pathogenesis of the disease. However, high levels of antibodies are present in the cerebral spinal fluid of patients with MS, and some theories predict that the B-cell response leading to antibody production is important for mediating the disease.

Autoimmune thyroid disease results from the production of autoantibodies that either stimulate the thyroid to cause hyperthyroidism (Graves' disease) or destroy the thyroid to cause hypothyroidism (Hashimoto's thyroiditis). Stimulation of the thyroid is caused by autoantibodies that bind and activate the thyroid stimulating hormone (TSH) receptor. Destruction of the thyroid is caused by autoantibodies that react with other thyroid antigens.

Additional diseases, disorders, and conditions amenable to the benefits provided by the compositions and methods of the invention include Sjogren's syndrome is an autoimmune disease characterized by destruction of the body's moisture-producing glands. Further, immune thrombocytopenic purpura (ITP) is caused by autoantibodies that bind to blood platelets and cause their destruction, and this condition is suitable for application of the materials and methods of the invention. Myasthenia Gravis (MG), a chronic autoimmune neuromuscular disorder characterized by autoantibodies that bind to acetylcholine receptors expressed at neuromuscular junctions leading to weakness of the voluntary muscle groups, is a disease having symptoms that are treatable using the composition and methods of the invention, and it is expected that the invention will be beneficial in treating and/or preventing MG. Still further, Rous Sarcoma Virus infections are expected to be amenable to treatment, or amelioration of at least one symptom, with the compositions and methods of the invention.

Another aspect of the present invention is using the materials and methods of the invention to prevent and/or treat any hyperproliferative condition of the skin including psoriasis and contact dermatitis or other hyperproliferative disease. Psoriasis, is characterized by autoimmune inflammation in the skin and is also associated with arthritis in 30% of cases, as well as scleroderma, inflammatory bowel disease, including Crohn's disease and ulcerative colitis. It has been demonstrated that patients with psoriasis and contact dermatitis have elevated antigen activity within these lesions (Ogoshi et al., J. Inv. Dermatol., 110:818-23 p yyzj). ne multispecific, multivalent binding proteins can deliver a cytotoxic cell of the immune system, for example, directly to cells within the lesions expressing high levels of antigen. The multivalent, e.g., multispecific, binding proteins can be administered subcutaneously in the vicinity of the lesions, or by using any of the various routes of administration described herein and others which are well known to those of skill in the art.

Also contemplated is the treatment of idiopathic inflammatory myopathy (IIM), including dermatomyositis (DM) and polymyositis (PM). Inflammatory myopathies have been categorized using a number of classification schemes. Miller's classification schema (Miller, Rheum Dis Clin North Am. 20:811-826, 1994) identifies 2 idiopathic inflammatory myopathies (IIM), polymyositis (PM) and dermatomyositis (DM).

Polymyositis and dermatomyositis are chronic, debilitating inflammatory diseases that involve muscle and, in the case of DM, skin. These disorders are rare, with a reported annual incidence of approximately 5 to 10 cases per million adults and 0.6 to 3.2 cases per million children per year in the United States (Targoff, Curr Probl Dermatol. 1991, 3:131-180). Idiopathic inflammatory myopathy is associated with significant morbidity and mortality, with up to half of affected adults noted to have suffered significant impairment (Gottdiener et al., Am J Cardiol. 1978, 41:1141-49). Miller (Rheum Dis Clin North Am. 1994, 20:811-826 and Arthritis and Allied Conditions, Ch. 75, Eds. Koopman and Moreland, Lippincott Williams and Wilkins, 2005) sets out five groups of criteria used to diagnose IIM, i.e., Idiopathic Inflammatory Myopathy Criteria (IIMC) assessment, including muscle weakness, muscle biopsy evidence of degeneration, elevation of serum levels of muscle associated enzymes, electromagnetic triad of myopathy, evidence of rashes in dermatomyositis, and also includes evidence of autoantibodies as a secondary criteria.

IIM associated factors, including muscle-associated enzymes and autoantibodies include, but are not limited to, creatine kinase (CK), lactate dehydrogenase, aldolase, C-reactive protein, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and antinuclear autoantibody (ANA), myositis specific antibodies (MSA), and antibody to extractable nuclear antigens.

Preferred autoimmune diseases amenable to the methods orine invenuon include Crohn's disease, Guillain-Barr6 syndrome (GBS; also known as acute inflammatory demyelinating polyneuropathy, acute idiopathic polyradiculoneuritis, acute idiopathic polyneuritis and Landry's ascending paralysis), lupus erythematosus, multiple sclerosis, myasthenia gravis, optic neuritis, psoriasis, rheumatoid arthritis, hyperthyroidism (e.g., Graves' disease), hypothyroidism (e.g., Hashimoto's disease), Ord's thyroiditis (a thyroiditis similar to Hashimoto's disease), diabetes mellitus (type 1), aplastic anemia, Reiter's syndrome, autoimmune hepatitis, primary biliary cirrhosis, antiphospholipid antibody syndrome (APS), opsoclonus myoclonus syndrome (OMS), temporal arteritis (also known as "giant cell arteritis"), acute disseminated encephalomyelitis (ADEM), Goodpasture's syndrome, Wegener's granulomatosis, coeliac disease, pemphigus, canine polyarthritis, warm autoimmune hemolytic anemia. In addition, the invention contemplates methods for the treatment, or amelioration of a symptom associated with, the following diseases, endometriosis, interstitial cystitis, neuromyotonia, scleroderma, vitiligo, vulvodynia, Chagas' disease leading to Chagasic cardiopathy (cardiomegaly), sarcoidosis, chronic fatigue syndrome, and dysautonomia.

The complement system is believed to play a role in many diseases with an immune component, such as Alzheimer's disease, asthma, lupus erythematosus, various forms of arthritis, autoimmune heart disease and multiple sclerosis, all of which are contemplated as diseases, disorders or conditions amenable to treatment or symptom amelioration using the methods according to the invention.

Certain constant sub-regions are preferred, depending on the particular effector function or functions to be exhibited by a multivalent single-chain binding molecule. For example, IgG (IgG1, 2, or 3) and IgM are preferred for complement activation, IgG of any subtype is preferred for opsonization and toxin neutralization; IgA is preferred for pathogen binding; and IgE for binding of such parasites as worms.

By way of example, FcRs recognizing the constant region of IgG antibodies have been found on human leukocytes as three distinct types of Fcy receptors, which are distinguishable by structural and functional properties, as well as by antigenic structures detected by CD monoclonal antibodies. They are known as rcyu, rcyiui, and FcyRIII, and are differentially expressed on (overlapping) subsets of leukocytes.

FcgRI (CD64), a high-affinity receptor expressed on monocytes, macrophages, neutrophils, myeloid precursors and dendritic cells, comprised isoforms la and lb. FcgRI has a high affinity for monomeric human IgG Iand IgG3. Its affinity for IgG4 is about 10 times lower, while it does not bind IgG2. FcgRI does not show genetic polymorphism.

FcyRII (CD32), comprised of isoforms Ila, llbl, lb2, 11b3 and 11c, is the most widely distributed human FcyR type, being expressed on most types of blood leukocytes, as well as on Langerhans cells, dendritic cells and platelets. FcyRII is a low-affinity receptor that only binds aggregated IgG. It is the only FeyR class able to bind IgG2. FcyRIIa shows genetics polymorphism, resulting in two distinct allotypes, FcyRIla-H131 and FcyRIla-R131, respectively. This functional polymorphism is attributable to a single amino acid difference: a histidine (H) or an arginine (R) residue at position 131, which is critical for IgG binding. FcyRlla readily binds human IgG and IgG3 and appears not to bind IgG4. The FcyRla-H131 has a much higher affinity for complexed IgG2 than the FcyRlla-R131 allotype.

FcyRIII (CD16) has two isoforms or allelotypes, both of which are able to bind IgGI and IgG3. The FcyRIIa, with an intermediate affinity for IgG, is expressed on macrophages, monocytes, natural killer (NK) cells and subsets of T cells. FcyRIIIb is a low-affinity receptor for IgG, selectively expressed on neutrophils. It is a highly mobile receptor with efficient collaboration with other membrane receptors. Studies with myeloma IgG dimers have shown that only IgG Iand IgG3 bind to FeyRIIIb (with low affinity), while no binding of IgG2 and IgG4 has been found. The FcyRJIIb bears a co-dominant, bi-allelic polymorphism, the allotypes being designated NA I(Neutrophil Antigen) and NA2.

Yet another aspect of the invention is use of the materials and methods of the invention to combat, by treating, preventing or mitigating the effects of, infection, resulting from any of a wide variety of infectious agents. The multivalent, multispecific binding molecules of the invention are designed to efficiently and effectively recruit the host organism's immune system to resist infection arising from a foreign organism, a foreign cell, a foreign virus or a foreign inanimate ooject. ror example, a multispecific binding molecule may have one binding domain that specifically binds to a target on an infectious agent and another binding domain that specifically binds to a target on an Antigen Presenting Cell, such as CD 40, CD80, CD86, DC-SIGN, DEC-205, CD83, and the like). Alternatively, each binding domain of a multivalent binding molecule may specifically bind to an infectious agent, thereby more effectively neutralizing the agent. In addition, the invention contemplates multispecific, multivalent binding molecules that specifically bind to a target on an infectious agent and to a non-cell-associated binding partner, which may be effective in conjunction with an effector function of the multispecific binding molecule in treating or preventing infection arising from an infectious agent.

Infectious cells contemplated by the invention include any known infectious cell, including but not limited to any of a variety of bacteria (e.g., pathogenic E. coli, S. typhimurium, P. aeruginosa, B. anthracis, C. botulinum, C. difficile, C. perfringens, H. pylori, V cholerae, and the like), mycobacteria, mycoplasma, fungi (including yeast and molds), and parasites (including any known parasitic member of the Protozoa, Trematoda, Cestoda and Nematoda). Infectious viruses include, but are not limited to, eukaryotic viruses (e.g., adenovirus, bunyavirus, herpesvirus, papovavirus, paramyxovirus, picornavirus, poxvirus, reovirus, retroviruses, and the like) as well as bacteriophage. Foreign objects include objects entering an organism, preferably a human, regardless of mode of entry and regardless of whether harm is intended. In view of the increasing prevalence of multi-drug-resistant infectious agents (e.g., bacteria), particularly as the causative agents of nosocomial infection, the materials and methods of the invention, providing an approach to treatment that avoids the difficulties imposed by increasing antibiotic resistance.

Diseases, conditions or disorders associated with infectious agents and amenable to treatment (prophylactic or therapeutic) with the materials and methods disclosed herein include, but are not limited to, anthrax, aspergillosis, bacterial meningitis, bacterial pneumoniae (e.g., chlamydia pneumoniae), blastomycosis, botulism, brucellosis, candidiasis, cholera, ciccidioidomycosis, cryptococcosis, diahhreagenic, enterohemorrhagic or enterotoxigenic E. coli, diphtheria, glanders, histoplasmosis, legionellosis, leprosy, listeriosis, nocardiosis, pertussis, salmonellosis, scarlet fever, sporotrichosis, strep throat, toxic shock syndrome, traveier s marrnea, and typhoid fever.

Additional aspects and details of the invention will be apparent from the following examples, which are intended to be illustrative rather than limiting. Example 1 describes recombinant cloning of immunoglobulin heavy and light chain variable regions. Example 2 describes the construction of Small Modular ImmunoPharmaceuticals. Example 3 describes the construction of a prototype cassette for a multivalent binding protein with effector function. Example 4 describes binding and expression studies with this initial prototype molecule. Example 5 describes construction of alternative constructs derived from this initial prototype molecule where the sequence of the linker region between the EFD and BD2 was changed in both length and sequence. In addition, it describes alternative forms where the orientation of V regions in binding domain 2 were also altered. Example 6 describes subsequent binding and functional studies on these alternative constructs with variant linker forms, identifying a cleavage in the linker region in several of these derivative forms, and the new sequence variants developed to address this problem. Example 7 describes the construction of an alternative preferred embodiment of the multispecific, multivalent fusion proteins, where both BD I and BD2 bind to antigens on the same cell type (CD20 and CD37), or another multispecific fusion protein where the antigen binding specificity for BD2 has been changed to human CD3 instead of CD28. Example 8 describes the binding and functional studies performed with the CD20-hIgG-CD37 multispecific constructs. Example 9 describes the binding and functional studies with the CD20-hIgG-CD3 multivalent fusion protein constructs. Example 10 discloses multivalent binding molecules having linkers based on specific regions of the extracellular domains of members of the immunoglobulin superfamily. Example 11 discloses assays for identifying binding domains expected to be effective in multivalent binding molecules in achieving at least one beneficial effect identified as being associated with such molecules (e.g., disease treatment).

Example 1

Cloning ofImmunoglobulin Heavy and Light Chain Variable Regions

Any methods known in the art can be used to elicit antibodies to a given antigenic target. Further, any methods known in the art can be used to clone the immunoglobulin light and/or heavy chain variable regions, as well as the constant sub-region of an antibody or antibodies. The following method provides an exemplary cloning method.

A. Isolation of Total RNA

To clone the immunoglobulin heavy and light chain variable regions, or the constant sub-region, total RNA is isolated from hybridoma cells secreting the appropriate antibody. Cells (2x10 7) from the hybridoma cell line are washed with 1x PBS and pelleted via centrifugation in a 12 x 75mm round bottom polypropylene tube (Falcon no. 2059). TRIolTMTotal RNA Isolation Reagent (Gibco BRL, Life Technologies, Cat no. 15596-018) is added (8 ml) to each tube and the cells are lysed via repeated pipetting. The lysate is incubated for 5 minutes at room temperature prior to the addition of 1.6 ml (0.2 x volume) of chloroform and vigorous shaking for 15 seconds. After standing 3 minutes at room temperature, the lysates are centrifuged at 9,000 rpm for 15 minutes in a 4°C pre-chilled Beckman JA-17 rotor in order to separate the aqueous and organic phases. The top aqueous phase (about 4.8 ml) is transferred into a new tube and mixed gently with 4 ml of isopropanol. After a 10 minute incubation at room temperature, the RNA is precipitated by centrifugation at 9,000 rpm in a 4°C JA-17 rotor for 11 minutes. The RNA pellet is washed with 8 ml of ice-cold 75% ethanol and re-pelleting by centrifugation at 7,000 x rpm for 7 minutes in a JA-17 rotor at 4°C. The ethanol wash is decanted and the RNA pellets are air-dried for 10 minutes. The RNA pellets are resuspended in 150 pl of diethylpyrocarbonate (DEPC)-treated ddH 20 containing 1 l of RNase Inhibitor (Catalog No. 799017; Boehringer Mannheim/Roche) per 1 ml of DEPC-treated ddH 20. The pellets are resuspended by gentle pipetting and are incubated for 20 minutesat55°C. RNA samples are quantitated by measuring the OD 2 6 0nm of diluted aliquots (1.0 OD 260 nn unit = 40 pg/ml RNA).

B. Rapid Amplification of cDNA Ends

5' RACE is carried out to amplify the ends of the heavy and light chain variable regions, or the constant sub-region. The 5' RACE System for Rapid Amplification of cDNA Ends Kit version 2.0 (Life Technologies, cat. no, 18374-058) is used according to the manufacturer's instructions. Degenerate 5' RACE oligonucleotide primers are designed to match, e.g., the constant regions of two common classes of mouse immunoglobulin heavy chains (IgG1 and IgG2b) using the oligonucleotide design program Oligo version 5.1 (Molecular Biology Insights, Cascade CO). Primers are also designed to match the constant region of the mouse IgG kappa light chain. This is the only class of immunoglobulin light chain, so no degeneracy is needed in the primer design. The sequences of the primers are as follows:

Name Sequence SEQ ID NO

Heavy Chain GSPI

5'AGGTGCTGGAGGGGACAGTCACTGAGCTGC3' 7

Nested Heavy Chain

5'GTCACWGTCACTGRCTCAGGGAARTAGC3' 8

(W = A or T; R = A or G)

Light Chain GSP1

5'GGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGC3'9

Nested Light Chain 5'CAAGAAGCACACGACTG

AGGCACCTCCAGATG3' 10

5' Race Abridged Anchor Primer

5'GGCCACGCGTCGACTAGTACGG

GNNGGGNNGGGNNG3' 11

To amplify the mouse immunoglobulin heavy chain component, the reverse transcriptase reaction is carried in a 0.2 ml thin-walled PCR tube containing 2.5 pmoles of heavy chain GSPi primer (SEQ ID NO: 7), 4 pg of total RNA isolated from a suitable hybridoma clone (e.g., either clone 4A5 or clone 45), and 12 l of DEPC treated ddH20. Likewise, for the mouse light chain component, the reverse transcriptase reaction is carried out in a 0.2 ml thin-walled PCR tube containing 2.5 pmoles of a light chain GSP1 primer (SEQ ID NO: 9), 4 g of total RNA from a suitable hybridoma clone (e.g., either clone 4A5 or clone 4B5), and 12 pl of DEPC treated ddH20.

The reactions are carried out in a PTC-100 programmable thermal cycler (MJ research Inc., Waltham, MA). The mixture is incubated at 70°C for 10 minutes to denature the RNA and then chilled on wet ice for 1 minute. The tubes are centrifuged briefly in order to collect moisture from the lids of the tubes. Subsequently, the following components are added to the reaction: 2.5 1 oflOx PCR buffer (200 mM Tris-HCl, pH 8.4, 500 mM KCI), 2.5 1 of 25 mMMgCl 2 , 1 pl of 10 mM dNTP mix, and 2.5 1 of 0.1 M DTT. After mixing each tube by gentle pipetting, the tubes are placed in a PTC-100 thermocycler at 42°C for 1 minute to pre-warm the mix. Subsequently, I pl (200 units) of SuperScriptTM II Reverse Transcriptase (Gibco BRL; cat no. 18089-011) is added to each tube, gently mixed by pipetting, and incubated for 45 minutes at 42°C. The reactions are cycled to 70°C for 15 minutes to terminate the reaction, and then cycled to 37°C. RNase mix (1pl) is then added to each reaction tube, gently mixed, and incubated at 37C for 30 minutes.

The first-strand cDNA generated by the reverse transcriptase reaction is purified with the GlassMAX DNA Isolation Spin Cartridge (Gibco-BRL) according to the manufacturer's instructions. To each first-strand reaction, 120 p1 of 6 M Nal binding solution is added. The cDNA/NaI solution is then transferred into a GlassMAX spin cartridge and centrifuged for 20 seconds at 13,000 x g. The cartridge inserts are carefully removed and the flow-through is discarded from the tubes. The spin cartridges are then placed back into the empty tubes and 0.4 ml of cold (4'C) lx wash buffer is added to each spin cartridge. The tubes are centrirugea at i,uuu x g for 20 seconds and the flow-through is discarded. This wash step is repeated three additional times. The GlassMAX cartridges are then washed 4 times with 0.4 ml of cold (4°C) 70% ethanol. After the flow-through from the final 70% ethanol wash is discarded, the cartridges are placed back in the tubes and centrifuged at 13,000 x g for an additional 1 minute in order to completely dry the cartridges. The spin cartridge inserts are then transferred to a fresh sample recovery tube where 50 l of 65°C (pre heated) DEPC-treated ddH 20 is quickly added to each spin cartridge. The cartridges are centrifuged at 13,000 x g for 30 seconds to elute the cDNA.

C. Terminal Deoxynucleotidyl Transferse (TdT) Tailing

For each first-strand cDNA sample, the following components are added to a 0.2 ml thin-walled PCR tube: 6.5 1 of DEPC-treated ddH 20, 5.0 pl of 5x tailing buffer, 2.5 l of 2 mM dCTP, and 10 1 of the appropriate GlassMAX-purified cDNA sample. Each 24 1 reaction is incubated 2-3 minutes in a thermal cycler at 94°C to denature the DNA, and chilled on wet ice for 1 minute. The contents of the tube are collected by brief centrifugation. Subsequently, 1 1 of terminal deoxynucleotidyl transferase (TdT) is added to each tube. The tubes are mixed via gentle pipetting and incubated for 10 minutes at 37'C in a PTC-100 thermal cycler. Following this 10 minute incubation, the TdT is heat inactivated by cycling to 65°C for 10 minutes. The reactions are cooled on ice and the TdT-tailed first-strand cDNA is stored at -20°C.

D. PCR of dC-tailed First-Strand cDNA

Duplicate PCR amplifications (two independent PCR reactions for each dC tailed first-strand cDNA sample) are performed in a 50pl volume containing 200 M dNTPs, 0.4 M of 5' RACE Abridged Anchor Primer (SEQ ID NO: 11), and 0.4 pM of either Nested Heavy Chain GSP2 (SEQ ID NO: 8) or Nested Light Chain GSP2 (SEQ ID NO: 10), 10 mM Tris-HCl (pH 8.3),1.5 mM MgCl 2,50 mM KCl, 5 pl of dC-tailed cDNA, and 5 units of ExpandTM Hi-Fi DNA polymerase (Roche/ Boehringer Mannheim GmbH, Germany). The PCR reactions are amplified using a "Touch-down/Touch-up" annealing temperature protocol in a PTC-100 programmable thermal cycler (MJ Research Inc.) with the following conditions: initial denaturation of 95'C for 40 seconds, 5 cycles at 94°C for 20 seconds, 61°C 2°C/cycle for 20 seconds, 72°C for 40 seconds + I second/cycle, followed by 5 cycles at 94C for 25 seconds, 53°C +1°C/cycle for 20 seconds, 72°C for 1o seconas - i second/cycle, followed by 20 cycles at 94°C for 25 seconds, 55°C for 20 seconds, 72°C for 51 seconds + 1 second/cycle, and a final incubation of 72°C for 5 minutes.

E. TOPO TA-Cloning

The resulting PCR products are gel-purified from a 1.0% agarose gel using the QIAQuick Gel purification system (QIAGEN Inc., Chatsworth, CA), TA-cloned into pCR2.1 using the TOPO TA Cloning@ kit (Invitrogen, San Diego, CA, cat. no. K4550-40), and transformed into E. coli TOPl0F' cells (Invitrogen), according to manufacturers' instructions. Clones with inserts are identified by blue/white screening according to the manufacturer's instructions, where white clones are considered positive clones. Cultures of 3.5 ml liquid Luria Broth (LB) containing 50 Rg/ml ampicillin are inoculated with white colonies and grown at 37°C overnight (about 16 hours) with shaking at 225 rpm.

The QIAGEN Plasmid Miniprep Kit (QIAGEN Inc., cat. no. 12125) is used to purify plasmid DNA from the cultures according to the manufacturer's instructions. The plasmid DNA is suspended in 34 [ of lx TE buffer (pH 8.0) and then positive clones sequenced as previously described by fluorescent dideoxy nucleotide sequencing and automated detection using ABI Big Dye Terminator 3.1 reagents at 1:4-1:8 dilutions and analyzed using an ABI 3100 DNA sequencer. Sequencing primers used include T7 (5'GTAATACGACTCACTATAGG3'; SEQ ID NO: 12) and M13 Reverse (5'CAGGAAACAGCTATGACC3'; SEQ ID NO: 13) primers. Sequencing results will confirm that the clones correspond to mouse IgG sequences.

F. De novo gene synthesis using overlapping oligonucleotide extension PCR

This method involves the use of overlapping oligonucleotide primers and PCR using either a high fidelity DNA polymerase or a mix of polymerases to synthesize an immunoglobulin V-region or other gene. Starting at the middle of the V-region sequence, 40-50 base primers are designed such that the growing chain is extended by 20-30 bases, in either direction, and contiguous primers overlap by a minimum of 20 bases. Each PCR step requires two primers, one priming on the anti-sense strand (forward or sense primer) and one priming on the sense strand (reverse or anti-sense primer) to create a growing double-stranded PCR product. During primer design, changes can be made in the nucleotide sequence of the final prouct to create restriction enzyme sites, destroy existing restriction enzyme sites, add flexible linkers, change, delete or insert bases that alter the amino acid sequence, optimize the overall DNA sequence to enhance primer synthesis and conform to codon usage rules for the organism contemplated for use in expressing the synthetic gene.

Primer pairs are combined and diluted such that the first pair are at 5 PM an each subsequent pair has a 2-fold greater concentration up to 80 M. One L from each of these primer mixes is amplified in a 50 tL PCR reaction using Platinum PCR SuperMix-High Fidelity (Invitrogen, San Diego, CA, cat. no. 12532-016). After a 2 minute initial denaturation at 94°C, 30 cycles of PCR are performed using a cycling profile of 94°C for 20 seconds, 60°C for 10 seconds; and 68°C for 15 seconds. PCR products are purified using Qiaquick PCR Purification columns (Qiagen Inc., cat. no. 28704) to remove excess primers and enzyme. This PCR product is then reamplified with the next set of similarly diluted primer pairs using PCR conditions exactly as described above, but increasing the extension time of each cycle to 68°C for 30 seconds. The resultant PCR product is again purified from primers and enzymes as described above and TOPO-TA cloned and sequenced exactly as described in section E above.

Example 2

Construction ofSmall Modular ImnunoPharmaceuticals(SMIPs)

A multispecific, multivalent binding protein with effector function was constructed that contained a binding domain I in the form of a single-chain recombinant (murine/human) scFv designated 2H7 (VL-linker-VH). The scFv 2H7 is a small modular immunopharmacaceutical (SMIP) that specifically recognizes CD20. The binding domain was based on a publicly available human CD20 antibody sequence GenBank Accession Numbers, M17953 for VH, and M17954 for VL. CD20-specific SMIPs are described in co-owned US Patent Publications 2003/133939, 2003/0118592 and 2005/0136049, incorporated herein in their entireties by reference. The peptide linker separating VL and VH was a 15-amino acid linker encoding the sequence: Asp-Gly 3 Ser-(Gly4 Ser)2 . Binding domain 1 was located at the N-terminus of the multispecific binding protein, with the C-termnus orimat domain linked directly to the N-terminus of a constant sub-region containing a hinge, CH2 and CH3 domains (in amino-to-carboxy orientation). The constant sub-region was derived from an IgG1 antibody, which was isolated by PCR amplification of human IgG1 from human PBMCs. The hinge region was modified by substituting three Ser residues in place of the three Cys residues present in the wild type version of the human IgG Ihinge domain, encoded by the 15 amino acid sequence: EPKSCDKTHTCPPCP (SEQ ID NO: 14; the three Cys residues replaced by Ser residues are indicated in bold). In alternative embodiments, the hinge region was modified at one or more of the cysteines, so that SSS and CSC type hinges were generated. In addition, the final proline was sometimes substituted with a serine as well as the cysteine substitutions.

The C-terminal end of the CH3 domain was covalently attached to a series of alternative linker domains juxtaposed between the constant sub-region C-terminus and the amino terminus of binding domain 2. Preferred multivalent binding proteins with effector function will have one of these linkers to space the constant sub-region from binding domain 2, although the linker is not an essential component of the compositions according to the invention, depending on the folding properties of BD2. For some specific multivalent molecules, the linker might be important for separation of domains, while for others it may be less important. The linker was attached to the N-terminal end of scFv 2E12 ((VH-inker-V), which specifically recognizes CD28. The linker separating the VH and VL domains of the scFv 2E12 part of the multivalent binding molecule was a 20-amino acid linker (Gly 4 Ser)4, rather than the standard (Gy4Ser)3 linker usually inserted between V domains of an scFv. The longer linker was observed to improve the binding properties of the 2e12 scFv in the VH-VL orientation.

The multispecific, multivalent binding molecule as constructed contained a binding domain 1, which comprises the 2E12 leader peptide sequence from amino acids 1-23 of SEQ ID NO: 171; the 2H7 murine anti-human CD20 light chain variable region, which is reflected at position 24 in SEQ ID NO: 171; an Asp-Gly3-Ser (Gly4Ser)2 linker, beginning at residue 130 in SEQ ID NO: 171, the 2H7 murine anti human CD20 heavy chain variable region with a leucine to serine (VHL11S) amino acid substitution at residue 11 in the variable domain for VH, anl wicn nas a single serine residue at the end of the heavy chain region (i.e., VTVS where a canonical sequence would be VTVSS) (Genbank Acc. No. M17953), and interposed between the two binding domains BDI (2H7) and BD2 (2E12) is a human IgG1 constant sub region, including a modified hinge region comprising a "CSC" or an "SSS" sequence, and wild-type CH2 and C3 domains. The nucleotide and amino acid sequences of the multivalent binding protein with effector function are set out in SEQ ID NOS: 228 and 229 for the CSC forms, respectively and SEQ ID NOS: 170 and 171, for the SSS forms.

Stably expressing cell lines were created by transfection via electroporation of either uncut or linearized, recombinant expression plasmid into Chinese hamster ovary cells (CHO DG44 cells) followed by selection in methotrexate containing medium. Bulk cultures and master wells producing the highest level of multivalent binding protein were amplified in increasing levels of methotrexate, and adapted cultures were subsequently cloned by limiting dilution. Transfected CHO cells producing the multivalent binding protein were cultured in bioreactors or wave bags using serum-free medium obtained from JRH Biosciences (Excell 302, cat. no. 14324 1000M, supplemented with 4 mM glutamine (Invitrogen, 25030-081), sodium pyruvate (Invitrogen 11360-070, diluted to IX), non-essential amino acids (Invitrogen, 11140-050, final dilution to IX), penicillin-streptromycin 100 IU/ml (Invitrogen, 15140-122), and recombulin insulin at 1 pg/ml (Invitrogen, 97-503311). Other serum free CHO basal medias may also be used for production, such as CD CHO, and the like. Fusion protein was purified from spent CHO culture supernatants by Protein A affinity chromatography. The multivalent binding protein was purified using a series of chromatography and filtration steps, including a virus reduction filter. Cell culture supernatants were filtered, then subjected to protein A Sepharose affinity chromatography over a GE Healthcare XK 16/40 column. After binding of protein to the column, the column was washed in dPBS, then 1.0 M NaCl, 20 mM sodium phosphate pH 6.0, and then 25 mM NaCl, 25 mN NaOAc, pH 5.0 to remove nonspecific binding proteins. Bound protein was eluted from the column in 100 mM Glycine (Sigma), pH 3.5, and brought to pH 5.0 with 0.5 M 2-(N-Morpholino) ethanesulfonic acid (MES), pH 6.0. Protein samples were concentrated to 25 mg/ml in preparation for GPC purification. Size exclusion chromatograpny was periormeu on a GE Healthcare AKTA Explorer 100 Air apparatus, using a GE healthcare XK column and Superdex 200 preparative grade (GE healthcare). The material was then concentrated and formulated with 20 mM sodium phosphate and 240 iM sucrose, with a resulting pH of 6.0. The composition was filtered before filling into sterile vials at various concentrations, depending on the amount of material recovered.

Example 3

Construction ofScorpion Expression Cassette

A nucleic acid containing the synthetic 2H7 scFv (anti-CD20; SEQ ID NO: 1) linked to a constant sub-region as described in Example 2 has been designated TRU 015. TRU-015 nucleic acid, as well as synthetic scFv 2E12 (anti-CD28 VL-VH; SEQ ID NO: 3) and synthetic scFv 2E12 (anti-CD28 VH-VL; SEQ ID NO: 5) nucleic acids encoding small modular immunopharmaceuticals, were used as templates for PCR amplification of the various components of the scorpion cassettes The template, or scaffold, for binding domain 1 and the constant sub-region was provided by TRU-015 (the nucleic acid encoding scFv 2H7 (anti-CD20) linked to the constant sub-region) and this template was constructed in the expression vector pD18. Theabove-noted nucleic acids containing scFv 2E12 in either of two orientations (VL-VH and VH-VL) provided the coding region for binding domain 2.

TRU 015 SSS hinge CmzCI for BD2/Linker Insertion

A version of the synthetic 2H7 scFv IgGI containing the SSS hinge was used to create a scorpion cassette by serving as the template for addition of an EcoRI site to replace the existing stop codon and XbaI site. This molecule was amplified by PCR using primer 9 (SEQ ID NO: 23; see Table 1) and primer 87 (SEQ ID NO: 40; see Table 1) as well as a Platinum PCR High Fidelity mix (Invitrogen). The resultant 1.5 Kbp fragment was purified and cloned into the vector pCR2.1-TOPO (Invitrogen), transformed into E. coli strain TOP10 (Invitrogen), and the DNA sequence verified.

Table 1

No. Name Sequence 5'-3' NO. PCR Primers GCGATAAAGCTTGCCGCCATGGAA 1 hVK3L-F3H3 GCACCAGCGCAGCTTCTCTTCC 15 ACCAGCGCAGCTTCTCTTCCTCCTG 2 hVK3L-F2 CTACTCTGGCTCCCAGATACCACCG 16 GGCTCCCAGATACCACCGGTCAAAT 3 hVK3L-FI-2H7VL TGTTCTCTCCCAGTCTCCAG 17 GCGATAGCTAGCCAGGCTTATCTAC 4 2H7VH-NheF AGCAGTCTGG 18 GCGATAGCTAGCCCCACCTCCTCCA G4S-NheR GATCCACCACCGCCCGAG 19 GCGTACTCGAGGAGACGGTGACCGT 6 015VH-XhoR GGTCCCTGTG 20 GCAGTCTCGAGCGAGCCCAAATCTTG 7 G1H-C-XHO TGACAAAACTC 21 GCAGTCTCGAGCGAGCCCAAATCTTC 8 G1H-S-XHO TGACAAAACTC 22 GCGTGAGAATTCTTACCCGGAGACAGG 9 CH3R-EcoRl GAGAGGCTC 23 GCGACGTCTAGAGTCATTTACCCGGAG GI-XBA-R ACAGG 24 AATTATGGTGGCGGTGGCTCGGGCGGT 11 G4SLinkRI-S GGTGGATCTGGAGGAGGTGGGAGTGGG 25 AATTCCCACTCCCACCTCCTCCAGATCCA 12 G4SLinkRI-AS CCACCGCCCGAGCCACCGCCACCAT 26 GCGTGTCTAGATTAACGTTTGATTTCCAG 13 2EI2VLXbaR CTTGGTG 27 GCGATGAATTCTGACATTGTGCTCACCCA 14 2EI2VLR1F ATCTCC 28 GCGATGAATTCTCAGGTGCAGCTGAAGGA 2E12VHR1F GTCAG 29 GCGAGTCTAGATTAAGAGGAGACGGTGAC 16 2E12VHXbaR TGAGGTTC 30

17 2el2VHdXbaFl GGGTCTGGAGTGGCTGGGAATGATATG 31

18 2e12VHdXbaRI ATTCCCAGCCACTCCAGACCCTTTCCTG 32

19 IgBsrGlF GAGAACCACAGGTGTACACCCTG 33

IgBsrGlR GCAGGGTGTACACCTGTGGTTCTCG 34 Sequencing Primers

82 M13R CAGGAAACAGCTATGAC 35

83 M13F GTAAAACGACGGCCAGTG 36

84 T7 GTAATACGACTCACTATAGG 37

pDI8F-17 AACTAGAGAACCCACTG 38

86 pD18F-20 GCTAACTAGAGAACCCACTG 39

87 pDI8F-1 ATACGACTCACTATAGGG 40

88 pD18R-s GCTCTAGCATTTAGGTGAC 41 89 CH3seqFl CATGAGGCTCTGCACAAC

No. Name Sequence 5'-3' NO. 42

90 CH3seqF2 CCTCTACAGCAAGCTCAC 43

91 CH3seqR1 GGTTCTTGGTCAGCTCATC 44

92 CH3seqR2 GTGAGCTTGCTGTAGAGG 45

Table 1. Oligonuceotide primers used to construct CD20-CD28 scorpion cassette. Primers are separated into 2 groups, PCR and Sequencing. PCR primers were used to construct the cassette and sequencing primers were used to confirm the DNA sequence of all intermediates and final constructs.

n2H7 VK and human VK leader sequence fusion

Oligonucleotide-directed PCR mutagenesis was used to introduce an Agel (ACCGGT) restriction site at the 5' end of the coding region for TRU 015 VK and an Nhe I (GCTAGC) restriction site at the 3' end of the coding region for the (G4S)3 linker using primers 3 and 5 from Table 1. Since primer 3 also encodes the last 6 amino acids of the human VK3 leader (gb:X01668), overlapping PCR was used to sequentially add the N-terminal sequences of the leader including a consensus Kozak box and HinDIII (AAGCTT) restriction site using primers 1, 2 and 5 from Table 1.

n2H7 IG1 SSS hi

Primers 4 and 6 (SEQ ID NOS: 18 and 20, respectively; Table 1) were used to re-amplify the TRU-015 VH with an NheI site 5' to fuse with the VK for TRU-015 and an Xho I (5'-CTCGAG-3') site at the 3' end junction with the IgG hinge-CH2CH3 domains. Likewise, the IgGIhinge-CH2-CH3 region was amplified using primers 8 and 9 from Table 1, introducing a 5'Xho I site and replacing the existing 3' end with an EcoRI (5'-GAATTC-3') site for cloning, and destroying the stop codon to allow translation of Binding Domain 2 attached downstream of the CH3 domain.. This version of the scorpion cassette is distinguished from the previously described cassette by the prefix "n."

In addition to the multivalent binding protein described above, a protein according to the invention may have a binding domain, either binding domain I or 2 or both, that corresponds to a single variable region of an immunoglobulin. Exemplary embodiments of this aspect of the invention would include binding domains corresponding to the VH domain of a camelid antibody, or a smge moame or unmodified V region of another species antibody capable of binding to the target antigen, although any single variable domain is contemplated as useful in the proteins of the invention.

2E12 VL-VH and VH-VL constructions

In order to make the 2E12 scFvs compatible with the cassette, an internal Xba I (5'-TCTAGA-3') site had to be destroyed using overlapping oligonucleotide primers 17 and 18 from Table 1. These two primers in combination with primer pairs 14/16 (VL-VH) or 13/15 (VH-VL) were used to amplify the two oppositely oriented binding domains such that they both carried EcoRI and XbaI sites at their 5' and 3' ends, respectively. Primers 13 and 16 also encode a stop codon (TAA) immediately in front of the Xba I site.

2H7 SSS IgG1 2el2 LH/HL Construction

Effector Domain- Binding Domain 2 Linker addition. (STD linkers - STDI and STD2)

Complementary primers 11 and 12 from Table 1 were combined, heated to 70°C and slow-cooled to room temperature to allow annealing of the two strands. 5'phosphate groups were added using T4 polynucleotide kinase (Roche) in IX Ligation buffer with 1mM ATP(Roche) using the manufacturer's protocol. The resulting double stranded linker was then ligated into the EcoRI site between the coding regions for the IgGI C1 terminus and the beginning of Binding Domain 2 using T4 DNA ligase (Roche). The resultant DNA constructs were screened for the presence of an EcoRI site at the linker-BD2 junction and the nucleotide sequence GAATTA at the C 3

linker junction. The correct STD 1 linker construct was then re-digested with EcoRI and the linker ligation repeated to produce a molecule that had a linker composed of two (STD 2) identical iterations of the Lxi sequence. DNA constructs were again screened as above.

Example 4

Expression studies

Expression studies were performed on the nucleic acids described above that encode multivalent binding proteins with effector function. Nucleic acids encoding multivalent binding proteins were transiently transfected into COs cells and the transfected cells were maintained under well known conditions permissive for heterologous gene expression in these cells. DNA was transiently transfected into COS cells using PEI or DEAE-Dextran as previously described (PEI= Boussif 0. et aL., PNAS 92: 7297-7301, (1995), incorporated herein by reference; Pollard H. et al., JBC 273: 7507-7511, (1998), incorporated herein by reference). Multiple independent transfections of each new molecule were performed in order to determine the average expression level for each new form. For transfection by PEI, COS cells were plated onto 60 mm tissue culture plates in DMEM/10%FBS medium and incubated overnight so that they would be approximately 90% confluent on the day of transfection. Medium was changed to serum free DMEM containing no antibiotics and incubated for 4 hours. Transfection medium (4mlplate) contained serum free DMEM with 50 g PEI and 10-20 ug DNA plasmid of interest. Transfection medium was mixed by vortexing, incubated at room temperature for 15 minutes, and added to plates after aspirating the existing medium. Cultures were incubated for 3-7 days prior to collection of supernatants. Culture supernatants were assayed for protein expression by SDS-PAGE, Western blotting, binding verified by flow cytometry, and function assayed using a variety of assays including ADCC, CDC, and coculture experiments.

SDS-PAGE Anaysis and Western Blotting Anatysis

Samples were prepared either from crude culture supernatants (usually 30 p/well) or purified protein aliquots, containing 8 ug protein per well, and 2X Tris-Glycine SDS Buffer (Invitrogen) was added to a 1X final concentration. Ten (10) 1 SeeBlue Marker (Invitrogen, Carlsbad, CA) were run to provide MW size standards. The multivalent binding (fusion) protein variants were subjected to SDS-PAGE analysis on 4-20% Novex Tris-glycine gels (Invitrogen, San Diego, CA). Samples were loaded using Novex Tris-glycine SDS sample buffer (2X) under reducing or non reducing conditions after heating at 95C for 3 minutes, followed by electrophoresis at 175V for 60 minutes. Electrophoresis was performed using 1X Novex Tris-Glycine SDS Running Buffer (Invitrogen).

After electrophoresis, proteins were transferred to PVDF membranes using a semi-dry electroblotter apparatus (Ellard, Seattle, WA) for 1 hour at 100 mAmp. Western transfer buffers included the following three buffers present on saturated whatman filter paper, and stacked in succession: no. 1 contains 36.34 g/liter Tris, pH 10.4, and 20% methanol; no. 2 contains 3.02 g/liter Tris, pH 10.4, and 20% methanol; and no. 3 contains 3.03 g/liter Tris, pH 9.4, 5.25 g/literc-amino caproic acid, and 20% methanol. Membranes were blocked in BLOTTO=5% nonfat milk in PBS overnight with agitation. Membranes were incubated with HRP conjugated goat anti-human IgG (Fc specific, Caltag) at 5 ug/ml in BLOTTO for one hour, then washed 3 times for 15 minutes each in PBS-0,5% Tween 20. Wet membranes were incubated with ECL solution for 1 minute, followed by exposure to X-omat film for 20 seconds. Figure 2 shows a Western Blot of proteins expressed in COS cell culture supernatant (30 p/well) electrophoresed under non-reducing conditions. Lanes are indicated with markers 1-9 and contain the following samples: Lane 1 (cut off= See Blue Markers, kDa are indicated to the side of the blot. Lane 2= 2H7-sssIgG P238S/P33]S-STD1 2e12 VLVH; lane 3= 2H7-sssIgG P238S/P331S-STD1-2e12 VHVL, Lane 4=2H7 sssIgG P238S/P331S-STD2-2e12 VLVH; Lane 5=2H7-sssIgG P238S/P331S-STD2 2e12 VHVL; Lane 6=2e12 VLVH SMIP; Lane 7=2e12 VHVL SMIP; Lane 8=2H7 SMIP. 2H7 in these constructs is always in the VLVH orientation, sssIgG indicates the identity of the hinge/linker located at linker position 1 as shown in Figure 5, P238S/P331S indicates the version of human IgG1 with mutations from wild type (first aa listed) to mutant (second aa listed) and the amino acid position at which they occur in wild type human IgGICH2 and CH3 domains, STD1 indicates the 20-amino acid (18 + restriction site) linker located in linker position 2 as shown in Figure 5, and STD2 indicates the 38 amino acid (36+restriction site) linker located in linker position 2 as shown in Figure 6.

Binding Studies

Binding studies were performed to assess the bispecific binding properties of the CD20/CD28 multispecific, multivalent binding peptides. Initially, WIL2-S cells were added to 96 well plates and centrifuged to pellet cells. To the seeded plates, CD20/CD28 purified protein was added, using two-fold titrations across the plate from 20 g/ml down to 0.16 g/ml. A two-fold dilution series of TRU-015 (source of binding domain 1) purified protein was also added to seeded plate wells, the concentration of TRU-015 extending from 20 pg/ml down to 0.lo gmi. unewer containing no protein served as a background control.

Seeded plates containing the proteins were incubated on ice for one hour. Subsequently, the wells were washed once with 200 1 1% FBS in PBS. Goat anti human antibody labeled with FITC (Fc Sp) at 1:100 was then added to each well, and the plates were again incubated on ice for one hour. The plates were then washed once with 200 l 1% FBS in PBS and the cells were re-suspended in 200p1 1% FBS and analyzed by FACS.

To assess the binding properties of the anti-CD28 peptide 2E12 VVL, CD28 expressing CHO cells were plated by seeding in individual wells of a culture plate. The CD20/CD28 purified protein was then added to individual wells using a two-fold dilution scheme, extending from 20 pg/ml down to 0.16 gg/ml. The 2E12IgG-VHVL SMIP purified protein was added to individual seeded wells, again using a two-fold dilution scheme, i.e., from 20 pg/ml down to 0.16 pg/ml. One well received no protein to provide a background control. The plates were then incubated on ice for 1 1% FBS in PBS, and goat anti-human antibody one hour, washed once with 200 labeled with FITC (Fc Sp, CalTag, Burlingame, CA) at 1:100 was added to each well. The plates were again incubated on ice for one hour and subsequently washed once with 200 11% FBS in PBS. Following re-suspension of the cells in 200 R 1% FBS, FACS analysis was performed. The results showed that multivalent binding proteins with the N-terminal CD20 binding domain 1 bound CD20; those proteins having the C-terminal CD28 binding domain 2 in the N-VH-VL-C orientation also bound CD28.

The expressed proteins were shown to bind to CD20 presented on WIL-2S cells (see Figure 3) and to CD28 presented on CHO cells (refer to Figure 3) by flow cytometry (FACS), thereby demonstrating that either BD Ior BD2 could function to bind the specific target antigen. Each data set on the graphs in Figure 3 shows the binding of serial dilutions of the different multivalent binding (fusion) proteins over the titration ranges indicated. The data obtained using these initial constructs indicate that multivalent binding (fusion) proteins with the binding domain 2 version using 2e12 in the VHVL orientation express better and bind better to CD28 than the form in the VLVH orientation at equivalent concentrations.

Figure 4 shows a graphical presentation of the results of binaing stuames performed with purified proteins from each of these transfections/constructs. The figure shows binding profiles of the proteins to CD20 expressing WIL-2S cells, demonstrating that the multivalent molecule binds to CD20 as well as the single specificity SMIP at the same concentration. The top and bottom panels for Figure 5 show the binding profiles of the BD2 specificity (2e12=CD28) to CD28 CHO cells. For binding of binding domain 2 to CD28, the orientation of the V regions affected binding of the 2el2. 2H7-sss-hgG-STDl-2el2 multivalent binding proteins with the 2e12 in the VH-VL (HL) orientation showed binding at a level equivalent to the single specificity SMIP, while the 2e12 LH molecule showed less efficient binding at the same concentration.

Example 5

Constructionof Various Linker Forms ofthe Multivalent Fusion Proteins.

This example describes the construction of the different linker forms listed in the table shown in Figure 6.

Construction of CI-BD2 linkers HI through H7

To explore the effect of CH3 -BD2 linker length and composition on expression and binding of the scorpion molecules, an experiment was designed to compare the existing molecule 2H7sssIgGl-Lx-2e12HL to a larger set of similar constructs with different linkers, Using 2H7sssIgGl-Lxl-2e12HL as template, a series of PCR reactions were performed using the primers listed in Oligonucleotide Table 2, which created linkers that varied in length form 0 to 16 amino acids. These linkers were constructed as nucleic acid fragments that spanned the coding region for CH3 at the BsrGI site to the end of the nucleic acid encoding the linker-BD2 junction at the EcoRI site.

Table 2 SEQ ID No. Name Sequence 5'-3' NO. PCR Primers GCGATAGAATTCCCAGATCCACCACCGCCCGA 1 L1-11R GCCACCGCCACCATAATTC 46

GCGATAGAATTCCCAGAGCCACCGCCACJmPm 2 LI-6R ATTC 47 GCGTATGAATTCCCCGAGCCACCGCCACCCTTA 3 L3R CCCGGAGACAGG 48 GCGTATGAATTCCCAGATCCACCACCGCCCGAG 4 L4R CCACCGCCACCCTTAC 49 GCGTATGAATTCCCGCTGCCTCCTCCCCCAGATC 5 L5R CACCACCGCC 50 6 IgBsrGlF GAGAACCACAGGTGTACACCCTG 51 GCGATAGAATTCGGACAAGGTGGACACCCCTTAC 7 L-CPPCPR CCGGAGACAGGGAGAG 52

Table 2. Sequences of primers used to generate CH3-BD2 linker variants.

Figure 6 diagrams the schematic structure of a multivalent binding (fusion) protein and shows the orientation of the V regions for each binding domain, the sequence present at linker position 1 (only the Cys residues are listed), and the sequence and identifier for the linker(s) located at linker position 2 of the molecules.

Example 6

Binding andFunctionalStudies With VariantLinker Forms ofthe 2H7-IgG-2e12 Prototype Multivalent Fusion Proteins.

This example shows the results of a series of expression and binding studies on the "prototype" 2H7-sssIgG-Hx-2e12 VHVL construct with various linkers (HI H7) present in the linker position 2. Each of these proteins was expressed by large scale COS transient transfection and purification of the molecules using protein A affinity chromatography, as described in the previous examples. Purified proteins were then subjected to analyses including SDS-PAGE, Western blotting, binding studies analyzed by flow cytometry, and functional assays for biological activity.

Binding Studies Comparing the Different BD2 Orientations

Binding studies were performed as described in the previous examples, except that protein A-purified material was used, and a constant amount of binding (fusion) protein was used for each variant studied, i.e., 0.72 ug/ml. Figure 7 shows a columnar graph comparing the binding properties of each linker variant and 2e12 orientation variant to both CD20 and CD28 target cells. H1-H6 refer to constructs with the HI H6 linkers and 2e12 in the VH-VL orientation. LI-L6 refer to constructs with the HI H6 linkers and 2e12 in the VL-VH orientation. The data demonstrate that the binding domain 2 specificity for 2e12 binds much more efficiently when present in me il orientation (samples H1-H6) than when in the LH orientation (samples LJ-L6). The effect of linker length is complicated by the discovery, as shown in the next set of figures, that molecules with the longer linkers contain some single-specificity cleaved molecules which are missing the CD28 binding specificity at the carboxy terminus. Other experiments were performed which address the binding of selected linkers, with the results shown in Figures 10, 12, and 13.

SDS-PAGE Analysis of purified H1-H7 Linker Variants

Samples were prepared from purified protein aliquots, containing 8 g protein per well, and 2X Tris-Glycine SDS Buffer (Invitrogen) was added to a IX final concentration. For reduced samples/gels, OX reducing buffer was added to lX to samples plus Tris-Glycine SDS buffer. Ten (10) l SeeBlue Marker (Invitrogen, Carlsbad, CA) was run to provide MW size standards. The multivalent binding (fusion) protein variants were subjected to SDS-PAGE analyses on 4-20% Novex Tris-glycine gels (Invitrogen, San Diego, CA). Samples were loaded using Novex Tris-glycine SDS sample buffer (2X) under reducing or non-reducing conditions after heating at 95°C for 3 minutes, followed by electrophoresis at 175V for 60 minutes. Electrophoresis was performed using IX Novex Tris-Glycine SDS Running Buffer (Invitrogen). Gels were stained after electrophoresis in Coomassie SDS PAGE R-250 stain for 30 minutes with agitation, and destained for at least one hour. Figure 8 shows the nonreduced and reduced Coomassie stained gels of the [2H7-sss-hIgG P238S/P33lS-Hx-2e12 VHVL] multivalent binding (fusion) protein variants, along with TRU-015 and 2e12 HLSMIP as control samples. As the linker length is increased, the amount of protein running at approximately SMIP size (or 52 kDa) increases. The increase in the amount of protein in this band corresponds with a decrease in the amount of protein in the upper band running at about 90 kDa. The gel data indicate that the full-length molecule is being cleaved at or near the linker, to generate a molecule which is missing the BD2 region. A smaller BD2 fragment is not present, indicating (1) that the nucleotide sequence within the linker sequence may be creating a cryptic splice site that removes the smaller fragment from the spliced RNA transcript, or (2) that the protein is proteolytically cleaved after translation of the full size polypeptide, and that the smaller BD2 fragment is unstable, i.e., susceptible to proteolytic processing. Western blotting of some of these molecules indicates that the proteins all contain the CD20 BD1 sequence, but the smaller banal is missing tne CD28 BD2 reactivity. No smaller band migrating at "bare" scFv size (25-27 kDa) was observed on any gels or blots, indicating that this smaller peptide fragment is not present in the samples.

Western Blot Binding of BDl and BD2 by 2H7 specific Fab or CD28mlg

Figure 9 shows the results of Western blotting of the 2H7-sss-hgG-H6 multivalent binding (fusion) proteins compared to each single-specificity SMIP.

Electrophoresis was performed under non-reducing conditions, and without boiling samples prior to loading. After electrophoresis, proteins were transferred to PVDF membranes using a semi-dry electroblotter apparatus (Ellard, Seattle, WA) for 1 hour at 100 mAmp. Membranes were blocked in BLOTTO (5% nonfat milk in PBS) overnight with agitation. Figure 9A: Membranes were incubated with the AbyD02429.2, a Fab directed to the 2H7 antibody, at 5 pg/ml in BLOTTO for one hour, then washed 3 times for 5 minutes each in PBS-0.5% Tween 20. Membranes were then incubated in 6X His-HRP for one hour at a concentration of 0.5 pg/m. Blots were washed three times for 15 minutes each in PBST. Wet membranes were incubated with ECL solution for 1 minute, followed by exposure to X-omat film for 20 seconds.

Figure 9B: Membranes were incubated with CD28Ig (Ancell, Bayport, MN) at 10 pg/ml in BLOTTO, then washed three times for 15 minutes each in PBS-0.5% Tween 20. Membranes were then incubated in goat anti-mouse HRP conjugate (CalTag, Burlingame, CA) at 1:3000 in BLOTTO. Membranes were washed three times, for 15 minutes each, then incubated in ECL solution for 1 minute, followed by exposure to X-omat film for 20 seconds. The results from the Western blots indicated that the CD28 binding domain was present in the multivalent "monomer" fraction migrating at approximately 90 kDa, and in higher order forms. No band was detectable migrating at the position expected for a single SMIP or bare scFv size fragment. When the CD20 anti-idiotype Fab was used, a SMIP-sized fragment was detected, indicating the presence of a peptide fragment containing (2H7-sss-hIgG), and missing the CD28 scFv BD2 portion of the fusion protein.

Binding Studies on Selected Linkers

Figure 10 shows the results of binding studies performed on the purified 2H7-sss hIgG-Hx-2el2 fusion proteins. Binding studies were performed to assess the bispecific binding properties of the CD20/CD28 multispecific binding peptides. Initially, WIL2-S cells were plated using conventional techniques. To the seeded plates, CD20/CD28 purified protein was added, using two-fold titrations across the plate from 20 g/ml down to 0.16 pg/ml. A two-fold dilution series of TRU-015 (source of binding domain 1) purified protein was also added to seeded plate wells, the concentration of TRU-015 extending from 20 pig/ml down to 0.16 pg/ml. One well containing no protein served as a background control.

Seeded plates containing the proteins were incubated on ice for one hour. Subsequently, the wells were washed once with 200 p 1% FBS in PBS. Goat anti human antibody labeled with FITC (Fc Sp) at 1:100 was then added to each well, and the plates were again incubated on ice for one hour. The plates were then washed once with 200 l 1% FBS in PBS and the cells were re-suspended in 200 l 1% FBS and analyzed by FACS.

To assess the binding properties of the anti-CD28 peptide 2E12 VHVL, CD28 expressing CHO cells were plated by seeding in individual wells of a culture plate. The CD20/CD28 purified protein was then added to individual wells using a two-fold dilution scheme, extending from 20 pg/ml down to 0.16 pg/ml. The2E12IgGvHvL SMP purified protein was added to individual seeded wells, again using a two-fold dilution scheme, i.e., from 20 pg/ml down to 0.16 pg/ml. One well received no protein to provide a background control. The plates were then incubated on ice for one hour, washed once with 200p 1% FBS in PBS, and goat anti-human antibody labeled with FITC(Fc Sp) at 1:100 was added to each well. The plates were again incubated on ice for one hour and subsequently washed once with 200 l 1% FBS in PBS. Following re-suspension of the cells in 200p 1% FBS, FACS analysis was performed. The expressed proteins were shown to bind to CD20 presented on WIL 2S cells (see Figure 1OA) and to CD28 presented on CHO cells (refer to Figure OB) by flow cytometry faces) , thereby demonstrating that either BD1 or BD2 could function to bind the specific target antigen. In addition, the linker used (HI-H6) was not found to significantly affect binding avidity to target antigen.

SEC Fractionation of Multivalent Binding (Fusion) Proteins. i ne onaing (fusion) protein was purified from cell culture supematants by protein A Sepharose affinity chromatography over a GE Healthcare XK 16/40 column. After binding of protein to the column, the column was washed in dPBS, then 1.0 M NaCl, 20 mM sodium phosphate pH 6.0, and then 25 mM NaCl, 25 mN NaOAc, pH 5.0, to remove nonspecific binding proteins. Bound protein was eluted from the column in 100 mM Glycine (Sigma), pH 3.5, and brought to pH 5.0 with 0.5 M 2-(N-Morpholino) ethanesulfonic acid (MES), pH 6.0 Protein samples were concentrated to 25 mg/ml using conventional techniques in preparation for GPC purification. Size exclusion chromatography (SEC) was performed on a GE Healthcare AKTA Explorer 100 Air apparatus, using a GE healthcare XK column and Superdex 200, preparative grade (GE healthcare).

Figure 12 shows a table summarizing the results of SEC fractionation of the different binding (fusion) proteins. With increasing linker length, the complexity of the molecules in solution also increases, making it difficult to isolate peak of interest, or POI from higher order forms by HPLC. The H7 linker seems to resolve much of this complexity into a more homogeneous form in solution, so that the soluble forms migrate mostly as a single POI at approximately 172 kDa.

Additional Binding Studies

A second series of experiments was performed (see Figures 12 and 13) with a smaller subset of multivalent binding (fusion) proteins, this time comparing linkers H3, H6, and H7. The data demonstrate that the binding level drops more significantly for CD28 than for CD20 binding, but both drop slightly as linker length increases. Further, the data showed that the H7 linker exhibited the highest level of binding to both antigens. These data were obtained using protein A-purified multivalent binding (fusion) proteins, but were not further purified by SEC, so multiple forms of the molecules may have been present in solution. The results also indicated that the truncated form may have been less stable than the true multivalent polypeptide, since the binding curves do not appear to fully reflect the significant amount of single specificity form present in solution for linker H6.

Demonstration of Maltispecific Binding From a Single Molecule

An alternative binding assay was performed (see Figure 13), where binding to CD20 on the surface of WIL-2S cells was detected with a reagent specific for the CD28 BD2, thereby demonstrating that simultaneous binding may occur to both target antigens, engaging both BD 1 and BD2 on the same multispecific binding (fusion) protein (refer to Figure 12) This assay demonstrates the multispecific binding property of the proteins.

Example 7

ConstructionofMultispecific Binding (Fusion)Proteins With Alternative Specificities in BD2

In addition to the prototype CD20-CD28 multispecific binding molecule, two other forms were made with alternative binding domain 2 regions, including CD37 and CD3 binding domains. The molecules were also made with several of the linker domains described for the [2H7-sss-IgG-Hx/STDx-2el2 HL] multispecific binding (fusion) proteins. The construction of these additional multispecific binding (fusion) molecules are described below.

Anti-CD37 Binding Domain Construction

Table 3

No. Name Sequence SEQ ID NO. ACTGCTGCAGCTGGACCGCGCT 23 G281LH-NheR AGCTCCGCCGCCACCCGAC 53 GGCGGAGCTAGCGCGGTCCAGC 24 G281LH-NheF TGCAGCAGTCTGGACCTG 54 GCGATCACCGGTGACATCCAGAT 25 G281-LH-LPinF GACTCAGTCTCCAG 55 GCGATACTCGAGGAGACGGTGAC 26 G281-LH-HXhoR TGAGGTTCCTTGAC 56 GCGATCGAATTCAGACATCCAGAT 27 G281-LH-LEcoF GACTCAGTCTCCAG 57 GCGATTCTAGATTAGGAAGAGACG 28 G281-LH-HXbaR GTGACTGAGGTTCCTTGAC 58 GCGATAACCGGTGCGGTCCAGCTG 29 G281-HL-HF CAGCAGTCTGGAC 59 GACCCAGCACCGCCCGAGCCACCG CCACCAGAAGAGACGGTGACTGAGG 60 30 G281-HL-HR3 TTC ACTCCCGCCTCCTCCTGATCCGCCG 31 G281-HL-HR2 CCACCCGACCCACCACCGCCCGAG 61 GAGTCATCTGGATGTCGCTAGCACTC 32 G281-HL-HNheR CCGCCTCCTCCTGATC 62 33 G281-HL-LNheF ATCAGGAGGAGGCGGGAGTGCTAGC

GACATCCAGATGACTCAGTC GCGATACTCGAGCCTTTGATCTCCAG 34 G281-HL-LXhcR TTCGGTGCCTC 64 GCGATATCTAGACTCAACCTTTGATCT 35 G281-HL-LXbaR CCAGTTCGGTGCCTC 65 GCGATAGAATTCGCGGTCCAGCTGCA 36 G281-HL-EcoF GCAGTCTGGAC 66

Table 3. Oligonucleotide primers used to generate G28-1 anti-CD37 binding domains for both SMIP molecules and scorpions.

The G28-1 scFv (SEQ ID NO:102) was converted to the G28-1 LH SMIP by PCR using the primers in Table X above. Combining primers 23 and 25 with 10 ng G28-1 scFv, the VK was amplified for 30 cycles of 94C, 20 seconds, 58C, 15 seconds, 68C, 15 seconds using Platinum PCR Supermix Hi-Fidelity PCR mix (Invitrogen, Carlsbad, CA) in an ABI 9700 Thermalcycler. The product of this PCR had the restriction sites PinAl(Agel) at the 5' end of the VK and NheI at the end of the scFv (G4S)3 linker. The VH was similarly altered by combining primers 24 and 26 with 10 ng G28-1 scFv in a PCR run under the identical conditions as with the VK above. This PCR product had the restriction sites NheI at the 5' end of the VH and XhoI at the 3' end. Because significant sequence identity overlap was engineered into primers 23 and 24, the VK and VH were diluted 5-fold, then added at a 1:1 ratio to a PCR using the flanking primers 25 and 26 and a full-length scFv was amplified as above by lengthening the 68C extension time from 15 seconds to 45 seconds. This PCR product represented the entire G28-1 scFv as a PinAI-Xhol fragment and was purified by MinElute column (Qiagen, ) purification to remove excess primers, enzymes and salts. The eluate was digested to completion with PinAI (Invitrogen)and XhoI (Roche) in 1X H buffer (Roche, ) at 37C for 4 hours in a volume of 50 L. The digested PCR product was then electrophoresed in a 1% agarose gel, the fragment was removed from the gel and re-purified on a MinElute column using buffer QG and incubating the gel-buffer mix at 50C for 10 minutes with intermittent mixing to dissolve the agarose after which the purification on the column was identical for primer removal post-PCR. 3 pL PinAI-XhoI digested 028-1 LH was combined with 1I pL PinAI-XhoI digested pD18-n2H7sssIgG1 SMIP in a 10 pL reaction with 5 [L 2X LigaFast Ligation Buffer (Promega, Madison, WI) and 1 pL T4 DNA ligase (Roche), mixed well and incubated at room temperature for 10 minutes. 3 p.L of this ligation was then transformed into competent TOP 10 (Invitrogen)using the manufacturer's protocol. These transformants were plated on LB agar plates win 1vO jg/ml carbenicillin(Teknova, ) and incubated overnight at 37C. After 18 hours of growth, colonies were picked and inoculated into 1 ml T-Broth (Teknova, ) containing 100 pg/ml carbenicillin in a deep well 96-well plate and grown overnight in a 37C shaking incubator. After 18-24 hours of growth, DNA was isolated from each overnight culture using the QAprep 96 Turbo Kit (Qiagen) on the BioRobot8OOO (Qiagen). 10 pL from each clone was then digested with both HindIII and Xhol restriction enzymes in 1X B buffer in a 15 pL reaction volume. The digested DNA was electrophoresed on 1% agarose E-gels (Invitrogen, CA) for restriction site analysis. Clones that contained a HindIII-XhoI fragment of the correct size were sequence verified. The G28-1 HL SMIP was constructed in a similar manner by placing a PinAl site on the 5' end and a (G4S)4 linker ending in an Nhe I site of the G28-1 VH using primers 29, 30 31 and 32 from Table X above. The VK was altered by PCR using primers 33 and 34 from Table X such that an NheI site was introduced at the 5'end of the VK and XhoI at the 3'end. These PCRs were then combined as above and amplified with the flanking primers 29 and 34 to yield an intact G28-1 scFv DNA in the VH-VL orientation which was cloned into PinAI-XhoI digested pD18-(n2H7)sssIgG1 SMIP exactly as with the G28-1 LH SMIP.

2H7sssIgQ1-STD1-G28-1 LH/HL Construction

Using the G28-1 LH and G28-1 HL SMIPs as templates, the LH and HL anti CD37 binding domains were altered by PCR such that their flanking restriction sites were compatible with the scorpion cassette. An EcoRI site was introduced at the 5' end of each scFv using either primer 27 (LH) or 36 (HL) and a stop codon/ Xbal site at the 3' end using either primer 28 (LH) or 35 (HL). The resulting DNAs were cloned into EcoRI-Xbal digested pD18-2H7sssIgG-STD].

2H7sssIgQ1-Hx-G28-1 HL Construction

2H7sssIgG1-Hx-2e12 HL DNAs were digested with BsrGI and EcoRI and the 325 bp fragment consisting of the C-terminal end of the IgG1 and linker. These were substituted for the equivalent region in 2H7sssIgGl-STD1-G19-4 HL by removal of the STD1 linker using BsrGI-EcoRl and replacing it with the corresponding linkers from the 2H7sssIgG1-Hx-2e12 HL clones.

Anti-CD3 Binding domain Construction

Table 4

SEQ ID No. Name Sequence NO. GCGTATGAACCGGTGACATCCAGAT 37 194-LH-LF1 GACACAGACTACATC 67 ATCCAGATGACACAGACTACATCCTC 38 194-LF2 CCTGTCTGCCTCTCTGGGAGACAG 68 GTCTGCCTCTCTGGGAGACAGAGTCA 39 194-LF3 CCATCAGTTGCAGGGCAAGTCAGGAC 69 GTTGCAGGGCAAGTCAGGACATTCGC 40 194-LF4 AATTATTTAAACTGGTATCAGCAG 70 ATTTAAACTGGTATCAGCAGAAACCAG 41 194-LF5 ATGGAACTGTTAAACTCCTGATC 71 GAACTGTTAAACTCCTGATCTACTACA 42 194-LF6 CATCAAGATTACACTCAGGAGTC 72 CAAGATTACACTCAGGAGTCCCATCAA 43 194-LF7 GGTTCAGTGGCAGTGGGTCTGGAAC 73 CAGGTTGGCAATGGTGAGAGAATAATC 44 194-LR7 TGTTCCAGACCCACTGCCACTGAAC 74 GCAAAAGTAAGTGGCAATATCTTCTGGT 45 194-LR6 TGCAGGTTGGCAATGGTGAGAG 75 GAACGTCCACGGAAGCGTATTACCC 46 194-LR5 TGTTGGCAAAAGTAAGTGGCAATATC 76 CGTTTGGTTACCAGTTTGGTGCCTCCAC 47 194-LR4 CGAACGTCCACGGAAGCGTATTAC 77 ACCACCGCCCGAGCCACCGCCACC 48 194-LR3 CCGTTTGGTTACCAGTTTGGTG 78 GCTAGCGCTCCCACCTCCTCCAGATCCA 49 194-LR2 CCACCGCCCGAGCCACCGCCAC 79 GTTGCAGCTGGACCTCGCTAGCGCT 50 194-LH-LRI CCCACCTCCTCCAGATC 80 GATCTGGAGGAGGTGGGAGCGCTAGC 51 194-LH-HF1 GAGGTCCAGCTGCAACAGTCTGGACCTG 81 AGCTGCAACAGTCTGGACCTGAACT 52 194-HF2 GGTGAAGCCTGGAGCTTCAATGAAG 82 AGCCTGGAGCTTCAATGAAGATTTCC 53 194-HF3 TGCAAGGCCTCTGGTTACTCATTC 83 GCAAGGCCTCTGGTTACTCATTCACT 54 194-HF4 GGCTACATCGTGAACTGGCTGAAGCAG 84 ATCGTGAACTGGCTGAAGCAGAGCC 55 194-HF5 ATGGAAAGAACCTTGAGTGGATTGGAC 85 GAACCTTGAGTGGATTGGACTTATTA 56 194-HF6 ATCCATACAAAGGTCTTACTACCTAC 86 AATGTGGCCTTGCCCTTGAATTTCTG 57 194-HR6 GTTGTAGGTAGTAAGACCTTTGTATG 87 CATGTAGGCTGTGCTGGATGACTTGT 58 194-HR5 CTACAGTTAATGTGGCCTTGCCCTTG 88 ACTGCAGAGTCTTCAGATGTCAGACTG 59 194-HR4 AGGAGCTCCATGTAGGCTGTGCTGGATG 89 ACCATAGTACCCAGATCTTGCACAG 60 194-HR3 TAATAGACTGCAGAGTCTTCAGATGTC 90 GCGCCCCAGACATCGAAGTACCAGTC 61 194-HR2 CGAGTCACCATAGTACCCAGATCTTG 91 GCGAATACTCGAGGAGACGGTGACCG 62 194-LH-HR1 TGGTCCCTGCGCCCCAGACATCGAAG 92 63 194-HL-HF1 GCGTATGAACCGGTGAGGTCCAGC

TGCAACAGTCTGGACCTG 93 ACCGCCACCAGAGGAGACGGTGACCGT 64 194-HL-HR1 GGTCCCTGCGCCCCAGACATCGAAGTAC 94 ACCTCCTCCAGATCCACCACCGCCCG 65 194-HL-HRO AGCCACCGCCACCAGAGGAGACGGTG 95 GCGGGGGAGGTGGCAGTGCTAGCGA 66 194-HL-LFI CATCCAGATGACACAGACTACATC 96 GCGAATACTCGAGCGTTTGGTTACCA 67 194-HL-LR3Xho GTTTGGTG 97 GCGATATCTAGATTACCGTTTGGTTAC 68 194-HL-LR3Xba CAGTTTGGTG 98 GCGTATGAGAATTCAGAGGTCCAGCTG 69 194-HL-HFIR1 CAACAGTCTGGACCTG 99 GCGTATGAGAATTCTGACATCCAGA 70 194-LH-LF1R1 TGACACAGACTACATC 100 GCGTATCTAGATTAGGAGACGGTGACC 71 194-LH-HR1Xba GTGGTCCCTGCGCCCCAGACATCGAAG 101

Table 4. Oligonucleotides used to generate anti-CD3 binding domain from the G19-4 scFv sequence.

The G19-4 binding domain was synthesized by extension of overlapping oligonucleotide primers as described previously. The light chain PCR was done in two steps, beginning by combining primers 43/44, 42/45, 41/46 and 40/47 at concentrations of SuM, 10 M, 20 M and 40 M ,respectively, in Platinum PCR Supermix Hi-Fidelity for 30 cycles of 94°C, 20 seconds, 60°C, 10 seconds, 68°C, 15 seconds. 1I L of the resultant PCR product was reamplified using a primer mix of 39/48 (10 pM), 38/49 (20 pM) and 37/50 (40 pM) for the LH or 66/67 (40 RM) for the HL orientation, using the same PCR conditions with the exception of the 68C extension which was increased to 25 seconds. The VK in the LH orientation was bounded by PinAI at the 5' end and NheI at the 3' end, while the HL orientation had NheI at the 5' end and Xhol at the 3' end.

To synthesize the heavy chain, primer mixes with the same concentrations as above were prepared by combining primers 56/57, 55/58, 54/59 and 53/60 for the first PCR step. In the second PCR, primers 52/61 (20 pM) and 51/62 (50 pM) were amplified with 1 pl from the first PCR using the same PCR conditions as with the second PCR of the light chain to make the LH orientation with NheI at the 5' end and Xhol at the 3'end, Primers 52/61(10 pM), 63/64 (20 RM), 63 (20 RM)/65 (40 VM) and 63(20 [M)/5 (80 VM) were combined in a second PCR with luL from the previous PCR to create the heavy chain in the HL orientation with PinAI at the 5' end and NheI at the 3' end. As with previous constructs, sufficient overlap was designed into the primers centered around the NheI site such that the G19-4 LH was synthesized by combining the heavy and light chain PCRs in the LH orientation and reamplifying with the flanking primers, 37 and 62 and the G19-4 HL was synthesized by combining the HL PCRs and re-amplifying with primers 63 and 67.

Full-length G19-4 LH/HL PCR products were separated by agarose gel electrophoresis, excised from the gel and purified with Qiagen MinElute columns as described earlier. These DNAs were then TOPO-cloned into pCR21 (Invitrogen), transformed into TOP10 and colonies screened first by EcoRi fragment size, then by DNA sequencing. G19-4 LH/HL were then cloned into pD8-IgG via PinAI-Xho for expression in mammalian cells.

2H7ssslaGI-STDI-G19-4 LH/HL Construction

Using the G19-4 LH and G19-4 HL SMIPs as templates, the LH and HL anti CD3 binding domains were altered by PCR such that their flanking restriction sites were compatible with the scorpion cassette. An EcoRI site was introduced at the 5' end of each scFv using either primer 27 (LH) or 36 (HL) and a stop codon/ Xbal site at the 3' end using either primer 28 (LH) or 35 (HL). The resulting DNAs were cloned into EcoRI-Xbal digested pD18-2H7sssgG-STD1.

2H7sssIgGl-Hx-G19-4 HL Construction

2H7sssIgG1-Hx-2e12 HL DNAs were digested with BsrGI and EcoRI and the 325 bp fragment consisting of the C-terminal end of the IgG Iand linker. These were substituted for the equivalent region in 2H7sssIgG-STD1-G19-4 HL by removal of the STD1 linker using BsrGl-EcoRI and replacing it with the corresponding linkers from the 2H7sssIgGl-Hx-2e12 HL clones.

Apparent from a consideration of the variety of multivalent binding proteins disclosed herein are features of the molecules that are amenable to combination in forming the molecules of the invention. Those features include binding domain 1, a constant sub-region, including a hinge or hinge-like domain, a linker domain, and a binding domain 2. The intrinsic modularity in the design of these novel binding proteins makes it straightforward for one skilled in the art to manipulate the DNA sequence at the N-terminal and/or C-terminal ends of any desirable module such that it can be inserted at almost any position to create a new molecule exhibiting altered or enhanced functionality compared to the parental molecule(s) from which it was derived. For example, any binding domain derived from a member of the immunoglobulin superfamily is contemplated as either binding domain 1 or binding domain 2 of the molecules according to the invention. The derived binding domains include domains having amino acid sequences, and even encoding polynucleotide sequences, that have a one-to-one correspondence with the sequence of a member of the immunoglobulin superfamily, as well as variants and derivatives that preferably share 80%, 90%, 95%, 99%, or 99.5% sequence identity with a member of the immunoglobulin superfamily. These binding domains (1 and 2) are preferably linked to other modules of the molecules according to the invention through linkers that may vary in sequence and length as described elsewhere herein, provided that the linkers are sufficient to provide any spacing and flexibility necessary for the molecule to achieve a functional tertiary structure. Another module of the multivalent binding proteins is the hinge region, which may correspond to the hinge region of a member of the immunoglobulin superfamily, but may be a variant thereof, such as the "CSC" or "SSS" hinge regions described herein. Also, the constant sub-region comprises a module of the proteins according to the invention that may correspond to a sub-region of a constant region of an immunoglobulin superfamily member, as is typified by the structure of a hinge-CH 2 -CH3 constant sub-region. Variants and derivatives of constant sub-regions are also contemplated, preferably having amino acid sequences that share 80%, 90%, 95%, 99%, or 99.5% sequence identity with a member of the immunoglobulin superfamily.

Exemplary primary structures of the features of such molecules are presented in Table 5, which discloses the polynucleotide and cognate amino acid sequence of illustrative binding domains 1 and 2, as well as the primary structure of a constant sub-region, including a hinge or hinge-like domain, and a linker that may be interposed, e.g., between the C-terminal end of a constant sub-region and the N terminal end of a binding domain 2 region of a multivalent binding protein. Additional exemplars of the molecules according to the invention include the above described features wherein, e.g., either or both of binding domains 1 and 2 comprise a domain derived from a VL or VL-like domain of a member of the immunoglobulin superfamily and a VH or VH-like domain derived from the same or a different member of the immunoglobulin superfamily, with these domains separated by a linker typified by any of thelinkers disclosed herein. Contemplated are molecues in wnicn me orientation of these domains is VL-V or Va-VT for BD1 and/or B1D2. A more complete presentation of the primary structures of the various features of the multivalent binding molecules according to the invention is found in the table appended at the end of this disclosure. The invention further comprehends polynucleotides encoding such molecules.

Table 5

I "M

2H7 LH atggattttcaagtgcagattttcag mdfqvqifsflliasvimsrgqivls 1 (2) cttcctgctaatcagtgcttcagtca qspailsaspgekvtmtcrasssvsym taatgtccagaggacaaattgttctc hwyqqkpgsspkpwiyapsnlasgvpa tcccagtctccagcaatcctgtctgc rfsgsgsgtsysltisrveaedaatyy atctccaggggagaaggtcacaatga cqqwsfnpptfgagtklelkdgggsgg cttgcagggccagctcaagtgtaagt ggsggggssqaylqqsgaesvrpgasv tacatgcactggtaccagcagaagcc kmsckasgytftsynmhwvkqtprqgl aggatcctcccccaaaccctggattt ewigaiypgngdtsynqkfkgkatltv atgccccatccaacctggcttctgga dkssstaymqlssltsedsavyfcarv gtccctgctcgcttcagtggcagtgg vyysnsywyfdvwgtgttvtvs gtctgggacctcttactctctcacaa tcagcagagtggaggctgaagatgct gccacttattactgccagcagtggag ttttaacccacccacgttcggtgctg ggaccaagctggagctgaaagatggc ggtggctcgggcggtggtggatctgg aggaggtgggagctctcaggcttatc tacagcagtctggggctgagtcggtg aggcctggggcctcagtgaagatgtc ctgcaaggcttctggctacacattta ccagttacaatatgcactgggtaaag cagacacctagacagggcctggaatg gattggagctatttatccaggaaatg gtgatacttcctacaatcagaagttc aagggcaaggccacactgactgtaga caaatcctccagcacagcctacatgc agctcagcagcctgacatctgaagac tctgcggtctatttctgtgcaagagt ggtgtactatagtaactcttactggt acttcgatgtctggggcacagggacc jacggtcaccgtctct

RMAZ1 '' 1%

' 2e12 LH atggattttcaagtgcagattttcag MDFQVQIFSFLLISASVIMSRGVDIVL 3 (4) cttcctgctaatcagtgcttcagtca TQSPASLAVSLGQRATISCRASESVEY taatgtccagaggagtcgacattgtg YVTSLMQWYQQKPGQPPKLLISAASNV ctcacccaatctccagcttctttggc ESGVPARFSGSGSGTDFSLNIHPVEED tgtgtctctaggtcagagagccacca DIAMYFCQQSRKVPWTFGGGTKLEIKR tctcctgcagagccagtgaaagtgtt GGGGSGGGGSGGGGSQVQLKESGPGLV gaatattatgtcacaagtttaatgca APSQSLSITCTVSGFSLTGYGVNWVRQ gtggtaccaacagaaaccaggacagc PPGKGLEWLGMIWGDGSTDYNSALKSR cacccaaactcctcatctctgctgct LSITKDNSKSQVFLKMNSLQTDDTARY agcaacgtagaatctggggtccctgc YCARDGYSNFHYYVMDYWGQGTSVTVS caggtttagtggcagtgggtctggga S cagactttagcctcaacatccatcct gtggaggaggatgatattgcaatgta tttctgtcagcaaagtaggaaggttc catggacgttcggtggaggcaccaag ctggaaatcaaacggggtggcggtgg atccggcggaggtgggtcgggtggcg gcggatctcaggtgcagctgaaggag tcaggacctggcctggtggcgccctc acagagcctgtccatcacatgcaccg tctcagggttctcattaaccggctat ggtgtaaactgggttcgccagcctcc aggaaagggtctggagtggctgggaa tgatatggggtgatggaagcacagac tataattcagctctcaaatccagact atcgatcaccaaggacaactccaaga gccaagttttcttaaaaatgaacagt ctgcaaactgatgacacagccagata ctactgtgcccgagatggttatagta actttcattactatgttatggactac tggggtcaaggaacctcagtcaccgt ctcctct 2e12 HL atggattttcaagtgcagattttcag MDFQVQIFSFLLISASVIMSRGVQVQL 5 (6) cttcctgctaatcagtgcttcagtca KESGPGLVAPSQSLSITCTVSGFSLTG taatgtccagaggagtccaggtgcag YGVNWVRQPPGKGLEWLGMIWGDGSTD ctgaaggagtcaggacctggcctggt YNSALKSRLSITKDNSKSQVFLKMNSL ggcgccctcacagagcctgtccatca QTDDTARYYCARDGYSNFHYYVMDYWG catgcaccgtctcagggttctcatta QGTSVTVSSGGGGSGGGGSGGGGSGGG accggctatggtgtaaactgggttcg GSDIVLTQSPASLAVSLGQRATISCRA ccagcctccaggaaagggtctggagt SESVEYYVTSLMQWYQQKPGQPPKLLI ggctgggaatgatatggggtgatgga SAASNVESGVPARFSGSGSGTDFSLNI agcacagactataattcagctctcaa HPVEEDDIAMYFCQQSRKVPWTFGGGT atccagactatcgatcaccaaggaca KLEIKR actccaagagccaagttttcttaaaa atgaacagtctgcaaactgatgacac agccagatactactgtgcccgagatg gttatagtaactttcattactatgtt atggactactggggtcaaggaacctc agtcaccgtctcctctgggggtggag gctctggtggcggtggatccggcgga ggtgggtcgggtggcggcggatctga cattgtgctcacccaatctccagctt ctttggctgtgtctctaggtcagaga gccaccatctcctgcagagccagtga aagtgttgaatattatgtcacaagtt ___Itaatgcagtggtaccaacagaaaccal ggacagocacocaaactctcatctc tgctgotagcaaogtagaatctgggg tccctgccaggtttagtggcagtggg tctgggacagactttagcctcaacat ccatcctgtggaggaggatgatattg caatgtattttgtagcaaagtagg aaggttccatggacgttcggtggagg caccaagctggaaatcaaacgt ________________ _____

G28-1 accggtgaoatocaqatqactcagtc DIQMTQSPASLSASVGETVTITCRTSE 102 (103) LHtcoagootocctatctgcatctgtgg NVYSYLJAWYQQKQGKSPQLLVSFAKTL gagagactgtcaccatcacatgtoga AEGVPSRFSGSGSGTQFSLKISSLQPE acaagtgaaaatgtttaoagttattt DSGSYFCQHHSDNPWTFGGGTELEIKG ggottggtatcagcagaaacagggaa GGGSGGGGSGGGGSASAVQLQQSGPEL aatotootcagctctggttctttt EKPGASVKISCKASGYSFTGYNMNWVK goaaaaaccttagcagaaggtgtgco QNNGKSLEWIGNIDPYYGGTTYNRKFK atcaaggttcagtggcagtggatoag GKATLTVDKSSSTAYMQLKSLTSEDSA qoacacagttttctctgaagatoagc VYYCARSVGPMDYWGQGTSVTVS agoctgcagcctgaagattctggaag ttatttotgtoaaoatcattocgata atocgtggacgttcggtggaggcacc gaactggagatcaaaggtggoggtgg otoqggggtggtgggtcgggtggog goggatctgctagcgcagtccagctg cagcagtctggacctgagctggaaaa gcctggcgcttcagtgaagatttcct gcaaggcttctggttactoattcact ggctacaatatgaactgggtgaagca gaataatggaaagagoottgagtgga ttggaaatattgatoottattatggt ggtactacctacaaccggaagttcaa gggcaaggccacattgactgtagaca aatootccagcacagoctacatgcag ctcaagagtctgacatctgaggactc tgcagtetattactgtgcaagatogg tcggccctatggactactggggtcaa _______ggaacotoagtcacogtotcgag _______________

G28-1 acoggtgagqtccagotgcaacagtc EVQLQQSGPELVKPGASMKISCKASGY 104 (105) HL tgqaoctgaactggtgaagcotggag SFTGYIVNWLKQSHGKNLEWIGLINPY ottoaatgaagatttoctgoaaggcc KGLTTYNQKFKGKATLTVDKSSSTAYM tctggttactoattcactggotacat ELLSLTSEDSAVYYCARSGYYGDSDWY ogtgaactggotgaagcagagccatg FDVWGAGTTVTVSSGGGGSGGGGSGGG gaaagaaccttgagtggattggactt GSGGGGSASDIQMTQTTSSLSASLGDR attaatccatacaaaggtcttactac VTISCRASQDIRNYLNWYQQKPDGTVK otacaaccagaaattoaagggcaagg LLIYYTSRLHSGVPSRFSGSGSGTDYS ooacattaactgtagacaagtcatcc LTIANLQPEDIATYFGQQGNTLPWTFG aqcacagcctacatggagotcotcag GGTKLVTKRS totgacatotgaagactctgcagtot attactgtgcaagatctgggtactat ggtgactcggactggtacttcgatgt ctggggcgoagggaccacggtcaccg totcctctggtggcggtggotogggo ggtggtggatctggaggaggtgggag ogggggaggtggcagtgctagcgaca tocagatgacacagactacatoctcc ctgtctgcctctctgggagacagagt caccatcagttgcagggcaagtcagg _____ acattcgcaattatttaaactggtat cagcagaaaccagatggaactgttaa actcctgatctactacacatcaagat tacactcaggagtcccatcaaggttC agtggcagtgggtctggaacagatta ttctctcaccattgccaacctqcaac caqaagatattgccacttacttttgc caacagggtaatacgcttccgtggac gttcggtggaggcaccaaactggtaa _______ccaaacgctcgag

G19-4 accggtgacatccagatgacacagac DIQMTQTTSSLSASLGDRVTISCRASQ 106 (107) LH tacatcctccctgtctgcctctctgg DIRNYLNWYQQKPDGTVKLLIYYTSRL~ gagacagagtcaccatcagttqcagg HSGVPSRFSGSGSGTDYSLTIANLQPE gcaagtcaggacattcgcaattattt DIATYFCQQGNTLPWTFGGGTKLVTKR aaactggtatcagcagaaaccaqatg GGGGSGGGGSGGGGSASEVQLQQSGPE gaactgttaaactcctgatctactac LVKPGASMKISCKASGYSFTGYIVNWL acatcaagattacactcagqagtccc KQSHGKNLEWIGLINPYKGLTTYNQKF atcaaggttcagtqgcagtgggtctg KGKATLTVDKSSSTAYMELLSLTSEDS gaacagattattctctcaccattgcc AVYYCARSGYYGDSDWYFDVWGAGTTV aacctgcaaccagaagatattgccac TVSS ttacttttgccaacagggtaatacgc ttccgtggacgttcggtggaggcacc aaactggtaaccaaacggggtggcgg tggctcgggcggtggtggatctggag gaggtgggagcgctagcgaggtccag ctgcaacagtetggacctgaactggt gaagcctggagettcaatgaagattt cctgcaaggcctctggttactcattc actggctacatcgtgaactggctgaa gcagagccatggaaagaaccttgagt ggattggacttattaatccatacaaa gqtcttactacetacaaccagaaatt caagggcaaggccacattaactgtag acaagtcatccagcacagcctacatg gagctcctcagtctgacatctgaaga ctctgcagtctattactgtgcaagat ctgggtactatggtgactcggactgg tacttcgatgtctggggcgcagggac cacggtcaccgtctcctcgag _____

G19-4 accggtgaggtccagctgcaacagtc EVQLQQSGPELVKPGASMKISGKASGY 108 (109) HL tggacctgaactggtgaagoctggag SFTGYIVNWLJKQSHGKNLEWIGLINPY cttcaatgaagatttcctgcaagqcc KGLTTYNQKFKGKATLTVDKSSSTAYM tctggttactcattcactggctacat ELLSLTSEDSAVYYCARSGYYGDSDWY cgtgaactggctgaagcagagccatg FDVWGAGTTVTVSSGGGGSGGGGSGGG gaaagaaccttgagtggattggactt GSASDIQMTQTTSSLSASLGDRVTISC attaatccatacaaaggtcttactac RASQDIRNYLNWYQQKPDGTVKLLIYY ctacaaccagaaattcaaqqgcaagg TSRLH-SGVPSRFSGSGSGTDYSLTTAN ccacattaactgtagacaagtcatcc LQPEDIATYFCQQGNTLPWTFGGGTKL agcacagcctacatggagctcctcag VTKRS tctgacatctgaagactctgcagtct attactgtgcaagatctgggtactat ggtgactcggactggtacttcgatgt ctggggcgcagggaccacggtcaccg tctcctctggtggcggtqggtcgggc ggtggtggatctggaggaggtgggag _______cgctagcgacatccagatgacacaga_______________

Ctaratectccctgtctgcctetctg ggagacagaqtcaccatcagttgcag ggcaagtcaggac&ttcgcaattatt taaactggtatcagcagaaaccagat ggaactgttaaactcetgatctacta cacatcaagattaaactcaggagtc catcaaggttcagtggcagtgggtct qgaacagattattctctcaccattgc caacctgcaaccagaagatattgcca cttacttttgccaacagggtaalacg cttccgtggacgttcggtggaggcac caaactggtaaccaaacgetcgag I

: ::::: . . .......... .0.: A. S. 5C '.k o .... , C C CA C

.... ~C:: $ ~* ... Sk .:...,x:.......... ..,;.oY,. ....1.,w ses(s)- gagcccaaatcttctgacaaaact EPKSSDKTHTSPPSS 230 (231) hIgG1 cacacatctccaccgagctca csc(s)- gagcccaaatcttgtgacaaaact EPKSCDKTHTSPPCS 232 (233) hIgG1 cacacatctccaccgtgctca ssc(s)- gagcccaaatcttctgacaaaact EPKSSDKTHTSPPCS 110 {111 hIgGI cacacatctccaccgtgctca scc(s)- gagcccaaatcttctgacaaaact EPKSSDKTHTCPPCS 112 (113J hIgG1 cacacatgtccaccgtgctca cSS(s)- gagcccaaatcttgtgacaaaact EPKSCDKTHTSPPSS 114 (15) hIgG1 cacacatctccaccgagctca scs(s)- gagcccaaatcttgtgacaaaact EPKSSDKTHTCPPSS 116 (117) hIgGI cacacatgtccaccgagctca ccc(s)- gagcccaaatcttgtgacaaaact EPKSCDKTHTSPPCS 118 (119) hIgG1 cacacatgtccaccgtgctca ccc(p)- gagcccaaatcttgtgacaaaact EPKSCDKTHTSPPCP 120 (121) hIgG1 cacacatgtccaccgtgccca ess(p)- gagcccaaatcttctgacaaaact EPKSSDKTHTSPPSP 122 (123) hIgGI cacacatctccaccgagccca csc(p)- gagcccaaatcttgtgacaaaact EPKSCDKTHTSPPCP 124 (125) hIgGi cacacatctccaccgtgccca ssc(p)- gagcccaaatcttctgacaaaact EPKSSDKTHTSPPCP 126 (127) hIgGl cacacatctccaccgtgccca_ scc(p)- gagcccaaatcttctgacaaaact EPKSSDKTHTCPPCP 128 (1291 hIgG1 cacacatgtccaccgtgccca css(p)- gagcccaaatttggacaaaact EPKSCDKTHTSPPSP 130 (131) hIgG1 cacacatctccaccgagccca scs(p)- gagcccaaatettgtgacaaaact EPKSSDKTHTCPPSP 132 (133) hIgG1 cacacatgtccaccgagccca scppcp agttgtccaccgtgccca SCPPCP 134 (.135)

hIgGl gcacctgaactcctgggtggatcg APELLGGSSVFLFPPKPKDTLMIS 142 (143) (P238S) tcagtettcctcttccccccaaaa RTPEVTCVVVDVSHEDPEVKFNWY CH2CH cccaaggacaccctcatgatctcc VDGVEVHNAKTKPREEQYNSTYRV cggacccctgaggtcacatgegtg VSVLTVLHQDWLNGKEYKCKVSNK gtggtggacgtgagccacgaagac ALPAPIEKTISKAKGQPREPQVYT cctgaggtcaagttcaactggtac LPPSRDELTKNQVSLTCLVKGFYP gtggacggcgtggaggtgcataat SDIAVEWESNGQPENNYKTTPPVL gccaagacaaagccgcgggaggag DSDGSFFLYSKLTVDKSRWQQGNV cagtacaacagcacgtaccgtgtg FSCSVMHEALHNHYTQKSLSLSPG gtcagcgtcctcaccgtcctgcac K caggactggctgaatggcaaggag tacaagtgcaaggtctccaacaaa gccctcccagcccccatcgagaaa acaatctccaaagccaaagggcag ccccgagaaccacaggtgtacacc ctgcccccatcccgggatgagctg accaagaaccaggtcagcctgacc tgcctggtcaaaggcttctatccc agcgacatcgccgtggagtgggag agcaatgggcagccggagaacaac tacaagaccacgcctcccgtgctg gactccgacggctccttcttcctc tacagcaagctcaccgtggacaag agcaggtggcagcaggggaacgtc ttctcatgctccgtgatgcatgag gctctgcacaaccactacacgcag aagagcctctccc tgtctccgggtaaatga hIgGI gcacctgaactcctgggtggaccg APELLGGPSVFLFPPKPKDTLMIS 144 (145) (P331S) tcagtcttcctcttccccccaaaa RTPEVTCVVVDVSHEDPEVKFNWY CH2CHa cccaaggacaccctcatgatctcc VDGVEVHNAKTKPREEQYNSTYRV cggacccctgaggtcacatgcgtg VSVLTVLHQDWLNGKEYKCKVSNK gtggtggacgtgagccacgaagac ALPASIEKTISKAKGQPREPQVYT cctgaggtcaagttcaactggtac LPPSRDELTKNQVSLTCLVKGFYP gtggacggcgtggaggtgcataat SDIAVEWESNGQPENNYKTTPPVL gccaagacaaagccgcgggaggag DSDGSFFLYSKLTVDKSRWQQGNV cagtacaacagcacgtaccgtgtg FSCSVMHEALHNHYTQKSLSLSPG gtcagcgtcctcaccgtcctgcac K caggactggctgaatggcaaggag tacaagtgcaaggtctccaacaaa gccctcccagcctccatcgagaaa acaatctccaaagccaaagggcag ccccgagaaccacaggtgtacacc ctgcccccatcccgggatgagctg accaagaaccaggtcagcctgacc tgcctggtcaaaggcttctatccc agcgacatcgccgtggagtgggag agcaatgggcagccggagaacaac tacaagaccacgcctcccgtgctg gactccgacggctccttcttcctc tacagcaagctcaccgtggacaag agcaggtggcagcaggggaacgtc ttctcatgctccgtgatgcatgag gctctgcacaaccactacacgcag aagagcctctccc tgtctccgggtaaatga hIgG1 gcacctgaactcctgggtggatcg APELLGGSSVFLFPPKPKDTLMIS 146 (147) (P238S/ tcagtcttcctcttccccccaaaa RTPEVTCVVVDVSHEDPEVKFNWY P331S) cccaaggacaccctcatgatctcc VDGVEVHNAKTKPREEQYNSTYRV CH2CH3 cggacccctgaggtcacatgcgtg VSVLTVLHQDWLNGKEYKCKVSNK gtggtggacgtgagccacgaagac ALPASIEKTISKAKGQPREPQVYT cctgaggtcaagttcaactggtac LPPSRDELTKNQVSLTCLVKGFYP gtggacggcgtggaggtgcataat SDIAVEWESNGQPENNYKTTPPVL gccaagacaaagccgcgggaggag DSDGSFFLYSKLTVDKSRWQQGNV cagtacaacagcacgtaccgtgtg FSCSVMHEALHNHYTQKSLSLSPG _gtcagcgtcctcaccgtcctgcac, K caggactggctgaatggcaaggag tacaagtgcaaggtctccaacaaa gccctcccagcctccatcgagaaa acaatctccaaagccaaagggcag ccccgagaaccacaggtgtacacc ctgcccccatcccgggatgagctg accaagaaccaggtcagcctgacc tgcctggtcaaaggcttctatccc agcgacatcgccgtggagtgggag agcaatgggcagccggagaacaac tacaagaccacgcctcccgtgctg gactccgacggctccttcttcctc tacagcaagctcaccgtggacaag agcaggtggcagcaggggaacgtc ttctcatgctccgtgatgcatgag gctctgcacaaccactacacgcag aagagcctctccctgtctccgggt aaatga

..........................

STD1 aattatggtggcggtggctcgggc NYGGGGSGGGGSGGGGSGNS 148 (149) ggtggtggatctggaggaggtggg agtgggaattct STD2 aattatggtggcggtggctcggc NYGGGGSGGGGSGGGGSGNYGCG 150 (151) ggtggtggatctggaggaggt9gq SGGGGSGGGGSGNS agtgggaattatggtggcggtggc tcgggcggtggtggatctggagga ggtgggagtgggaattct H1 aattct NS 152 (153) H2 ggtggcggtggctcggggaattct GGGGSGNS 154 (155) H3 aattatggtggcggtggctctggg NYGGGGSGNS 156 (157) aattct H4 ggtggcggtggctcgggcggtggt GGGGSGGGGSGNS 158 (159) ggatctgggaattct H5 aattatggtggcggtggctcgggc NYGGGGSGGGGSGNS 160 (161) ggtggtggatctgggaattct H6 ggtggcggtggctcgggcggtggt GGGGSGGGGSGGGGSGNS 162 (163) gatctgggggaggaggcagcggg aattct H7 gggtgtccaccttgtccgaattct GCPPCPNS 164 (165) (G4S)3 ggtggcggtggatccggcggaggt GGGSGGGSGGGS 166 (167) gggtcgggtggcggcggatct (G4S)4 ggtqgcggtggctcgggcggtggt GGGSGGGSGGGSGGGGS 168 (169) ggatctggaggaggtgggagcggg ggaggtggcagt

Table 5. Primary structures (polynucoleotide and cognate amino acid sequences) of exemplary features of multivalent binding molecules.

Example 8

Binding and FunctionalStudies with Alternative Multispecific Fusion Proteins

Experiments that parallel the experiments described above for the prototypical CD20-IgG-CD28 multispecific binding (fusion) molecule were conducted for each of the additional multivalent binding molecules described above. In general, the data obtained for these additional molecules parallel the results observed for the prototype molecule. Some of the salient results of these experiments are disclosed below. Figure 14 shows results of blocking studies performed on one of the new molecules where both BD1 and BD2 bind to target antigens on the same cell or cell type, in this case, CD20 and CD37. This multispecific, multivalent binding (fusion) protein was designed with binding domain 1 binding CD20 (2H7; VLVH orientation), and binding domain 2 binding CD37, G28-1 VL-VH (LH) or VH-VL (HL). The experiment was performed in order to demonstrate the multispecific properties of the protein.

Blocking Studies:Ramos or BJAB B lymphoblastoid cells (2.5x10 5) were pre incubated in 96-well V-bottom plates in staining medium (PBS with 2% mouse sera) with murine anti-CD20 (25 ig/ml) antibody, or murine anti-CD37 (10 g/ml) antibody, both together or staining medium alone for 45 minutes on ice in the dark. Blocking antibodies were pre-incubated with cells for 10 minutes at room temperature prior to addition of the multispecific binding (fusion) protein at the concentration ranges indicated, usually from 0.02 [g/ml to 10 pg/ml, and incubated for a further 45 minutes on ice in the dark. Cells were washed 2 times in staining medium, and incubated for one hour on ice with Caltag (Burlingame, CA) FITC goat anti-human IgG (1:100) in staining medium, to detect binding of the multispecific binding (fusion) proteins to the cells. The cells were then washed 2 times with PBS and fixed with 1% paraformaldehyde (cat. no. 19943, USB, Cleveland, Ohio). The cells were analyzed by flow cytometry using a FACsCalibur instrument and CellQuest software (BD Biosciences, San Jose, CA). Each data series plots the binding of the 2H7-sss hIgG-STD1-G28-1 HL fusion protein in the presence of CD20, CD37, or both CD20 and CD37 blocking antibodies. Even though this experiment used one of the cleaved linkers, only the presence of both blocking antibodies completely eliminates binding by the multispecific binding (fusion) protein, demonstrating that the bulk of the molecules possess binding function for both CD20 and CD37. The data were similar for two cell lines tested in panels A and B, Ramos and BJAB, where the CD20 blocking antibody was more effective than the CD37 blocking antibody at reducing the level of binding observed by the multispecific binding (fusion) protein.

ADCC Assays

Figure 15 shows the results of ADCC assays performed on the CD20-CD37 multispecific binding (fusion) proteins. ADCC assays were performed using BJAB lymphoblastoid B cells as targets and human PBMC as effector cells. BJAB cells were labeled with 500 pCi/ml "Cr sodium chromate (250 pCi/pg) for 2 hours at 37°C inIMDM/10%FBS. The labeled cells were washed three times in RPMI.10% FBS and resuspended at 4x105 cells/ml in RPMI. Heparinized, human whole blood was obtained from anonymous, in-house donors and PBMC isolated by fractionation over Lymphocyte Separation Media (LSM, ICN Biomedical) gradients. Buffy coats were harvested and washed twice in RPMI/10% FBS prior to resuspension in RPMI/10% FBS at a final concentration of 5x106cells/ml. Cells were counted by trypan blue exclusion using a hemacytometer prior to use in subsequent assays. Reagent samples were added to RPMI medium with 10% FBS at 4 times the final concentration and three 10 fold serial dilutions for each reagent were prepared. These reagents were then added to 96-well U-bottom plates at 50g/well for the indicated final concentrations. The "Cr-labeled BJAB cells were added to the plates at 50 l/well (2x10 4 cells/well). The PBMCs were then added to the plates at 100 pl/well (5x105 cells/well) for a final ratio of 25:1 effector (PBMC):target (BJAB). Effectors and targets were added to medium alone to measure background killing. The "Cr-labeled cells were added to medium alone to measure spontaneous release of "Cr and to medium with 5% NP40 (cat. no.28324, Pierce, Rockford, IL) to measure maximal release of 1 Cr. Reactions were set up in triplicate wells of a 96-well plate. Multispecific binding (fusion) proteins were added to wells at a final concentration ranging from 0.01 g/ml to 10pg/ml, as indicated on the graphs. Each data series plots a different multispecific binding (fusion) protein or the corresponding single specificity SMIPs at the titration ranges described. Reactions were allowed to proceed for 6 hours at 37°C in 5% CO 2 prior to harvesting and counting. Twenty-five g of the supernatant from each well were then transferred to a Luma Plate 96 (cat. no. 6006633, Perkin Elmer, Boston, Mass) and dried overnight at room temperature. CPM released was measured on a Packard TopCounNXT. Percent specific killing was calculated by subtracting (cpm (mean of triplicate samples) of sample - cpm spontaneous release)/(cpm maximal release-cpm spontaneous release) xlOO. Data are plotted as % specific killing versus protein concentration. The data demonstrate that the multispecific binding (fusion) protein is able to mediate ADCU activity against cells expressing the target antigen(s) as well as the single specificity SMIPs for CD20 and/or CD37, but does not show augmentation in the level of this effector function.

Co-culture Experiments

Figure 16 shows the results of experiments designed to look at other properties of this type of multispecific binding (fusion) protein, where having two binding domains against targets expressed on the same cell or cell type might result in synergistic effects by signaling/binding through the two surface receptors bound. The co-culture experiments were performed using PBMC isolated as described for the ADCC assays above.. These PBMC were resuspended in culture medium at 2x10 6 cells/ml in a final volume of 500 p/well, and cultured alone or incubated with single specificity SMIPs for CD20, CD37, CD20+CD37, or the multispecific binding (fusion) protein using the H7 linker, [2H7-sss-IgG-H7-G28-1 HL. Each of the test reagents was added at a final concentration of 20 jig/mi. After 24 hours of culture, no real differences were seen in the % of B cells in culture; however, when the cells were subjected to flow cytometry, cell clumping was visible in the FWD X 90 staining pattern for the cultures containing the multispecific binding (fusion) protein, indicating that the B cells expressing the two target antigens were engaged in homotypic adhesion. After 72 hours in culture, the multispecific binding (fusion) protein resulted in the death of almost all the B cells present. The combination of the two single-specificity SMIPs also drastically decreased the percentage of B cells, but not to the level seen with the multispecific binding molecule. These data suggest that engaging both binding domains for CD20 and CD37 on the same multispecific molecule, results in homotypic adhesion between B cells and may also result in binding of both CD20 and CD37 antigens on the same cell. Without wishing to be bound by theory, the synergistic effect in eliminating target cells may be due (1) to the binding through binding domains 1 and 2 on the same cell types, and/or (2) to interactions of the effector function domain (constant sub-region) of the multivalent binding molecules with monocytes or other cell types in the PBMC culture that result in delayed killing. The kinetics of this killing effect are not rapid, taking more than 24 hours to be achieved, indicating that it is may be a secondary effect, requiring production of cytokines or other molecules prior to the effects being observed.

Apoptosis Assays

Figure 17 shows the results of experiments designed to explore the induction

of apoptosis after treatment of B cell lines with either the [2H7-sss-hgG-H7-G28-1 HL] multispecific, multivalent binding (fusion) proteins or the single specificity CD20 and/or CD37 SMIPS, alone and in combination with one another. Ramos cells (panel A; ATCC No. CRL-1596), and Daudi cells (panel B; ATCC No. CCL-213) were incubated overnight (24 hours) at 37°C in 5% CO 2 in Iscoves (Gibco) complete medium with 10% FBS at 3 X 105 cells/ml and 5, 10, or 20 g/ml fusion proteins. For combination experiments with the single specificity SMIPs, the proteins were used at the following concentrations: TRU-015 (CD20 directed SMIP)=10 pg/ml, with 5 g/ml G28-1 LH (CD37 directed SMIP). Alternatively, TRU-015=20 pg/ml was combined with G28-1 LH at 10 pg/mil. Cells were then stained with Annexin V FITC and propidium iodide using the BD Pharmingen Apoptosis Detection Kit I cat. no. 556547), and processed according to kit instructions. The cells were gently vortexed, incubated in the dark at room temperature for 15 minutes, and diluted in 400

pl binding buffer prior to analysis. Samples were analyzed by flow cytometry on a FACsCalibur (Becton Dickinson) instrument using Cell Quest software (Becton Dickinson). The data are presented as columnar graphs plotting the percentage of Annexin V/propidium iodide positive cells versus type of treatment. Clearly, the multispecific binding (fusion) protein is able to induce a significantly higher level of apoptotic death in both cell lines than the single specificity reagents, even when used together. This increased functional activity reflects an interaction of the coordinate binding of BDl and BD2 (specific for CD20 and CD37) receptors on the target cells.

Example 9

Binding and FunctionalPropertiesof 2H7-hIgG-G19-4Multispecific Binding (Fusion) Proteins

This example describes the binding and functional properties of the 2H7 hIgG-G19-4 multispecific fusion proteins. The construction of these molecules is described in Example 7. Expression and purification are as described in previous Examples.

Binding experiments were performed as described for previous molecules, except that the target cells used to measure CD3 binding were Jurkat cells expressing CD3 on their surface, Refer to Figure 18, where the top graph shows binding curves obtained for binding of the CD20-CD3 multispecific molecules to Jurkat cells using purified proteins serially diluted from 20 to 0.05 g/ml. The HL orientation of the G19-4 specificity seems to bind better to the CD3 antigen than does the LH orientation. The lower panel shows the binding curves obtained for the BD1, the binding domain recognizing CD20. All of the molecules bind well, and at a level nearly equivalent to a single specificity SMIP for CD20.

ADCC Assays

For the data presented in Figure 19, ADCC assays were performed as described in the previous Example. In this case, the fusion proteins were all 2H7 hIgG-G19-4 variants or combinations of the single-specificity SMIPs (2H7, specific for CD20) or antibodies (019-4, specific for CD3). In addition, for the data presented in the lower panel of Figure 19, NK cells were depleted from PBMC prior to use, by magnetic bead depletion using a MACS (Miltenyi Biotec, Auburn, CA) column separation apparatus and NK cell-specific CD16 magnetic microbeads (cat no.: 130 045-701). The data presented in the two panels demonstrate that all of the CD20 hIgG-CD3 multispecific molecules mediate ADCC, regardless of whether NK cells are depleted or total PBMC are used in the assay. For the TRU 015 or combinations of G19-4 and TRU015, only cultures containing NK cells could mediate ADCC. G19-4 did not work well in either assay against BJAB targets, which do not express CD3, although G19-4 may have bound to CD3 expressing NK T cells and activated these cells in the first assay shown. The killing observed in the lower panel for the multispecific binding (fusion) proteins is probably mediated through activation of cytotoxicity in the T cell population by binding CD3, against the BJAB targets expressing the CD20 antigen. This killing activity appears to be relatively insensitive to the dosage of the molecules over the concentration ranges used, and is still significantly different from the other molecules tested, even at a concentration of 0.01 ug/ml.

Multivalent Binding Molecules

Other embodiments include linker domains derived from immunoglobulins. More specifically, the source sequences for these linkers are sequences obtained by comparing regions present between the V-like domains or the V- and C-like domains of other members of the immunoglobulin superfamily. Because these sequences are usually expressed as part of the extracellular domain of cell surface receptors, they are expected to be more stable to proteolytic cleavage, and should also not be immunogenic. One type of sequence that is not expected to be as useful in the role of a linker for the multivalent binding (fusion) proteins is the type of sequence expressed on surface-expressed members of the -Ig superfamily, but that occur in the intervening region between the C-like domain and the transmembrane domain. Many of these molecules have been observed in soluble form, and are cleaved in these intervening regions close to the cell membrane, indicating that the sequences are more susceptible to cleavage than the rest of the molecule.

The linkers described above are inserted into either a single specificity SMIP, between the binding domain and the effector function domain, or are inserted into one of the two possible linker positions in a multivalent binding (fusion) protein, as described herein.

A complete listing of the sequences disclosed in this application is appended, and is incorporated herein by reference in its entirety. The color coding indicating the sequence of various regions or domains of the particular polynucleotides and polypeptides are useful in identifying a corresponding region or domain in the sequence of any of the molecules disclosed herein.

Example 11

Screening matrixforscorpion candidates targetingB-cells

Introduction

As a means of identifying combinations of paired monoclonal antibody binding domains that would most likely yield useful and potent multivalent binding molecules, or scorpions, against a target population, a series of monoclonal antibodies against B cell antigens was tested in a combination matrix against B cell lines representing various non Hodgkin's lymphomas. To ensure that all possible pairwise comparisons of antibodies known or expected to bind to the cell of interest are assayed, a two-dimensional matrix of antibodies may be used to guide the design of studies using a given cell type. Monoclonal antibodies against numerous B cell antigens known by their cluster designations (CDs) are recorded in the left column. Some of these antibodies (designated by the antigen(s) to which they specifically bind), i.e., CD19, CD20, CD21, CD22, CD23, CD30, CD37, CD40, CD70, CD72, CD79a, CD79b, CD80, CD81, CD86, and CL II (MHC Class II), were incubated, alone or in combination with other members of this monoclonal antibody set, with antigen-positive target cells. The variable domains of these antibodies are contemplated as binding domains in exemplary embodiments of the multivalent binding molecules. Using the knowledge in the art and routine procedures, those of skill in the art are able to identify suitable antibody sequences (nucleic acid encoding sequences as well as amino acid sequences), for example in publicly available databases, to generate a suitable antibody or fragment thereof (e.g., by hybridization based cloning, PCR, peptide synthesis, and the like), and to construct multivalent binding molecules using such compounds. Sources of exemplary antibodies from which binding domains were obtained as described herein are provided in Table 6. Typically, a cloning or synthesis strategy that realizes the CDR regions of an antibody chain will be used, although any antibody, fragment thereof, or derivative thereof that retains the capacity to specifically bind to a target antigen is contemplated.

Stated in more detail, the cloning of heavy and/or light chain variable regions of antibodies from hybridomas is standard in the art. There is no requirement that the sequence of the variable region of interest be known in order to obtain that region using conventional cloning techniques. See, e.g., Gilliland et al., Tissue Antigens 47(1):1-20 (1996). To prepare single-chain polypeptides comprising a variable region recognizing a murine or human leukocyte antigen, a method was devised for rapid cloning and expression that yielded functional protein within two to three weeks of RNA isolation from hybridoma cells. Variable regions were cloned by poly-G tailing the first-strand cDNA followed by anchor PCT with a forward poly-C anchor primer and a reverse primer specific for the constant region sequence. Both primers contain flanking restriction endonuclease sites for insertion into pUC19. Sets of PCR primers for isolation of murine, hamster and rat VL and VH genes were generated. Following determination of consensus sequences for a specific VL and VH pair, the VL and VH genes were linked by DNA encoding an intervening peptide linker (typically encoding (Gly 4Ser) 3) and the VL-linker-VH gene cassettes were transferred into the pCDM8 mammalian expression vector. The constructs were transfected into COS cells and sFvs were recovered from conditioned culture medium supernatant. This method has been successfully used to generate functional sFv to human CD2, CD3, CD4, CD8, CD28, CD40, CD45 and to murine CD3 and gp39, from hybridomas producing murine, rat, or hamster antibodies. Initially, the sFvs were expressed as fusion proteins with the hinge-CH2-CH3 domains of human IgG Ito facilitate rapid characterization and purification using goat anti-human IgG reagents or protein A. Active sFv could also be expressed with a small peptide, e.g., a tag, or in a tailless form. Expression of CD3 (G19-4) sFv tailless forms demonstrated increased cellular signaling activity and revealed that sFvs have potential for activating receptors.

Alternatively, identification of the primary amino acid sequence of the variable domains of monoclonal antibodies can be achieved directly, e.g., by limited proteolysis of the antibody followed by N-terminal peptide sequencing using, e.g., the Edman degradation method or by fragmentation mass spectroscopy. N-terminal sequencing methods are well known in the art. Following determination of the primary amino acid sequence, the variable domains, a cDNA encoding this sequence is assembled by synthetic nucleic acid synthesis methods (e.g., PCR) followed by scFv generation. The necessary or preferred nucleic acid manipulation methods are standard in the art.

Fragments, derivatives and analogs of antibodies, as described above, are also contemplated as suitable binding domains. Further, any of the constant sub-regions described above are contemplated, including constant sub-regions comprising any of the above-described hinge regions. Additionally, the multivalent single-chain binding molecules described in this example may include any or all of the linkers described herein.

Monoclonal antibodies were initially exposed to cells and then cross-linked using a goat anti-mouse second-step antibody (2nd step). Optionally, one could cross link the antibodies prior to contacting cells with the antibodies, e.g., by cross-linking the antibodies in solution. As another alternative, monoclonal antibodies could be cross-linked in a solid phase by adsorbing onto the plastic bottom of tissue culture wells or "trapped" on this plastic by means of goat anti-mouse antibody adsorbed to the plastic, followed by plate-based assays to evaluate, e.g., growth arrest or cell viability.

Inversion of phosphatidylserine from the cytosolic side of the cell membrane to the exterior cell surface of that plasma membrane is an accepted indicator of pro apoptotic events. Progression to apoptosis leads to loss of cell membrane integrity, which can be detected by entry of a cell-impermeant intercalating dye, e.g., propidium iodide (PI). Following cell exposure to monoclonal antibodies alone or in combination, a dual, pro-apoptotic assay was performed and treated cell populations were scored for cell surface-positive annexin V (ANN) and/or PI inclusion.

Annexin V binding /Propidium iodide internalization analysis

Cells and cell culture conditions. Experiments were performed to examine the effect of cross-linking two different monoclonal antibodies against targets expressed on four human B-cell lines. Effects on cell lines were measured by determining levels of ANN and/or PI staining following exposure. The human B cell lines BJAB, Ramos (ATCC#CRL-1596), Daudi (ATCC#CCL-213), and DHL-4 (DSMZ#ACC495) were incubated for 24 hours at 37C in 5% CO 2 in Iscoves (Gibco) complete medium with 10% FBS. Cells were maintained at a density between 2-8 x 105 cells/ml and a viability typically >95% prior to study.

Experiments were conducted at a cell density of 2 x 105 cells/ml and 2 g/ml of each comparative monoclonal antibody from a matrix against B-cell antigens. Each comparator monoclonal antibody was added at 2 g/ml alone or individually when combined with each matrix monoclonal antibody, also at 2 g/ml. Table 6 lists the catalog number and sources of monoclonal antibodies used in these experiments. For cross-linking these monoclonal antibodies in solution, goat anti-mouse IgG (Jackson Labs catalog no.115-001-008) was added to each well at a concentration ratio of 2:1 (goat anti-mouse: each monoclonal antibody), e.g., a well with only one monoclonal antibody at 2 g/ml would have goat anti-mouse added to a final concentration of 4 g/ml, while wells with both comparator monoclonal antibody (2 jig/ml) and a monoclonal antibody from the matrix (2 g/ml) would have 8 g/ml of goat anti-mouse antibody added to the well.

After 24 hours of incubation at 37°C in 5% CO 2 , cells were stained with Annexin V-FITC and propidium iodide using the BD Pharmingen Annexin V-FITC Apoptosis Detection Kit I (#556547). Briefly, cells were washed twice with cold PBS and resuspended in "binding buffer" at 1 x 106 cells/mi. One hundred microliters of the cells in binding buffer were then stained with 5 pl of Annexin V-FITC and 5 l of propidium iodide. The cells were gently mixed and incubated in the dark at room temperature for 15 minutes. Four hundred microliters of binding buffer were then added to each sample. The samples were then read on a FACsCalibur (Becton Dickinson) and analyzed using Cell Quest software (Becton Dickinson).

Table 6

Name Catalog number Commercial supplier Anti-CD19 #C2269-74 US Biological (Swampscott, MA) Anti-CD20 #169-820 Ancell Corp (Bayport. MN) Anti-CD21 #170-820 Ancell Corp (Bayport, MN) Anti-CD22 #171-820 Ancell Corn (Bayport. MN) Anti-CD23 #172-820 Ancell Corp (Bayport, MN) Anti-CD30 #179-820 Ancell Corp (Bayport, MN) Anti-CD37 #186-820 Ancell Corp (Byort, MN) Anti-CD40 #300-820 Ancell Corp (Bayport, MN) Anti-CD70 #222-820 Ancell Corp (Bayport. MN) Anti-CD72 #C2428-41B1 US Biological (Swampscott, MA) Anti-CD79a #235-820 Ancell Corp (Bayport, MN) Anti-CD79b #301-820 Ancell Corp (Bayport, MN) Anti-CD80 #110-820 Ancell Corp (Baort, MN) Anti-CD81 #302-820 Ancell Corp (Bayport, MN) Anti-CD86 #307-820 Ancell Corp (Bayport, MN) Anti-CL II DR, DO, DP #131-820 Ancell Cop (Bavport, MN)

Table 6. Antibodies against B cell antigens used in this study and their sources.

Addition of the cross-linking antibody (e.g., goat anti-mouse antibody) to monoclonal antibody A alone resulted in increased cell sensitivity, suggesting that a multivalent binding molecule, or scorpion, constructed with two binding domains recognizing the same antigen would be effective at increasing cell sensitivity. Without wishing to be bound by theory, this increased sensitivity could be due to antigen clustering and altered signaling. TNF receptor family members, for example, require homo-multimerization for signal transduction and scorpions with equivalent binding domains on each end of the molecule could facilitate this interaction. The clustering and subsequent signaling by CD40 is an example of this phenomenon in the B cell system.

As shown in Figures 20, 21 and 22, the addition of monoclonal antibody A and monoclonal antibody B against different antigens will produce additive or in some combinations greater than additive (i.e., synergistic) pro-apoptotic effects on treated cells. In Figure 20, for example, the combination of anti-CD20 with monoclonal antibodies against other B cell antigens all resulted, to varying extents, in increased cell sensitivity. Some combinations, such as anti-CD20 combined with anti-CD19 or anti-CD20 combined with anti-CD21, however, produced greater than additive pro-apoptotic effects, indicating that multivalent binding molecules or scorpions composed of these binding domains should be particularly effective at eliminating transformed B cells. Referring to Figure 20, the percentage of cells exhibiting pro-apoptotic activities when exposed to anti-CD 20 antibody alone is about 33% (vertically striped bar corresponding to "20," i.e., the anti-CD20 antibody); the percentage of pro-apoptotic cells upon exposure to anti-CD19 antibody is about 12% (vertically striped bar in Fig. 20 corresponding to "19," i.e., the anti-CD19 antibody); and the percentage of pro-apoptotic cells upon exposure to both anti-CD20 and anti-CD19 antibodies is about 73% (horizontally striped bar in Fig. 20 corresponding to "19"). The 73% of pro-apoptotic cells following exposure to both antibodies is significantly greater than the 45% (33%+ 12%) sum of the effects attributable to each individual antibody, indicating a synergistic effect attributable to the anti-CD19 and anti-CD 20 antibody pair. Useful multivalent binding molecules include molecules in which the two binding domains lead to an additive effect on B cell behavior as well as multivalent binding molecules in which the two binding domains lead to synergistic effects on B-cell behavior. In some embodiments, one binding domain will have no detectable effect on the measured parameter of cell behavior, with each of the paired binding domains contributing to distinct aspects of the activities of the multivalent binding molecule, such as a multispecific, multivalent binding molecule (e.g., binding domain A binds to a target cell and promotes apoptosis while binding domain B binds to a soluble therapeutic such as a cytotoxin). Depending on the design of a multivalent binding molecule, the issue of the type of combined effect (additive, synergistic, or inhibitory) of the two binding domains on a target cell may not be relevant because one of the binding domains is specific for a non-cellular (e.g., soluble) binding partner or is specific for a cell-associated binding partner, but on a different cell type.

Exemplary binding domain pairings producing additive, synergistic or inhibitory effects, as shown in Figures 20-23, are apparent from Tables 7 and 8. Table 7 provides quantitative data extracted from each of Figures 20-23 in terms of the percentage of cells staining positive for ANN and/or PI. Table 8 provides calculations using the data of Table 7 that provided a basis for determining whether the interaction of a given pair of antibodies yielded an additive, synergistic, or inhibitory effect, again as assessed by the percentage of cells staining positive for ANN and/or PI.

Table 7

Name Anti-CD20 Anti-CD79b Anti-CL 11 Anti-CD22

Anti-CD19 13/73* 18/76/66 14/47/46 12/11

Anti-CD20 33/NA 42/94/92 33/71/76 28/33

Anti-CD21 14/75 22/50/76 18/24/40 11/11

Anti-CD22 8/55 12/39/33 12/19/17 10/12

Anti-CD23 8/41 12/63/55 14/22/17 10/12

Anti-CD30 8/38 14/72/61 12/56/61 10/11

Anti-CD37 15/45 19/92/86 20/60/62 19/20

Anti-CD40 10/48 12/44/30 13/21/28 14/13

Anti-CD70 9/40 12/56/39 15/21/15 10/10

Anti-CD72 NA 16/60/64 30/78/63 17/17

Anti-CD79a 21/66 43/42/50 28/55/51 14/14

Anti-CD79b 46/88 70/70/68 45/80/76 26/16

Name Anti-CD20 Anti-CD79b Anti-CL II Anti-CD22

Anti-CD80 7/41 14/35/30 15/19/17 11/11

Anti-CD81 14/65 25/86/83 25/54/43 19/20

Anti-CD86 7/38 16/58/42 15/24/18 14/11

Anti- CL II 53/77 52/96/98 47/52/43 72/57

Incolumns 2-4 of Table 7, the numerical values reflect the heights of histogram bars in Figures 20-22, respectively, with the first number in each cell denoting the height of a vertically striped bar, the second number denoting the height of a horizontally striped bar and, where present, the third number reflecting the height of a stippled bar. In column 5, the first number reflects the height of a solid bars and the second number reflects the height of a slant-striped bar in Figure 23.

Table 8

Name Anti-CD20 Anti-CD79b Anti-CL II Anti-CD22

Anti-CD19 S:13+33=46' A: 18+56=74 S: 14+26=40 1: 12+10=22

A: 18+43=61 S: 14+18=32

Anti-CD20 NA A: 42+56=98 S: 33+26=59 A/JI:28+10=38

A:42+43=85 S:33+18=51

Anti-CD21 S:14+33=47 1:22+56=78 I:18+26=44 I:11+10=21

S:22+43=65 A: 18+18-36

Anti-CD22 S:8+33=41 1:12+56=68 I:12+26=38 NA

1:12+43=55 1:12+18=30

Anti-CD23 A:8+33=41 A:12+56=68 1:14+26=40 I:10+10=20

A:12+43=55 I:14+18=32

Name Anti-CD20 Anti-CD79b Anti-CL II Anti-CD22

Anti-CD30 A: 8+33=41 A: 14+56=70 S: 12+26=38 I: 10+10=20

A: 14+43=57 S: 12+18=30

Anti-CD37 A: 15+33=48 S: 19+56=75 S: 20+26=46 1: 19+10=29

S:19+43=62 S:20+18=38

Anti-CD40 A/S:10+33=43 1: 12+56=68 1:13+26=39 1:14+10=24

I:12+43=55 A:13+18=31

Anti-CD70 A:9+33=42 I: 12+56=68 1:15+26=41 1:10+10=20

I:12+43=55 1:15+18=33

Anti-CD72 NA 1:16+56=72 S:30+26=56 I: 17+10=27

A:16+43=59 S:30+18=48

Anti-CD79a S:21+33=54 I:43+56=99 A:28+26=54 1:14+10=24

I:43+43=86 A:28+18=46

Anti-CD79b S:46+33=79 NA S:45+26=71 I:26+10=36

S:45+18=63

Anti-CD80 A:7+33=40 I:14+56=70 1:15+26=41 I:11+10=21

1:14+43=57 1:15+18=33

Anti-CD81 S:14+33=47 A:25+56=81 A:25+26=51 1:19+10=29

S:25+43=68 A:25+18=43

Anti-CD86 A:7+33=40 I:16+56=72 1:15+26=41 1:14+11=25

1:16+43=59 I:15+18=33

Name Anti-CD20 Anti-CD79b Anti-CL II Anti-CD22

Anti- CL II I: 53+33=86 A: 52+56=108 NA I: 72+10=82

A: 52+43=95

"A" means an "additive" effect was observed "S" means a "synergistic" effect was observed "I" means an "inhibitory" effect was observed *Equation schematic: A+B=C, where "A" is the percent ANN and/or PI positive cells due to matrix antibody alone, "B" is the percent ANN and/or PI positive cells due to the common antibody (anti-CD20 for Fig. 20, anti-CD79b for Fig. 21, anti-CLII for Fig. 22, and anti-CD22 for Fig. 23), and "C" is the expected additive effect. (See Table 7, above, for the quantitative data corresponding to Figures 20-23.) Where two equations are present in a cell, the upper equation reflects results use of the higher indicated concentration of common antibody; the lower equation reflects use of the lower indicated concentration of common antibody.

In some embodiments, the two binding domains interact in an inhibitory, additive or synergistic manner in sensitizing (or de-sensitizing) a target cell such as a B cell. Figure 23 shows the protective, or inhibitory, effects resulting from combining anti-CD22 antibody with strongly pro-apoptotic monoclonal antibodies such as the anti-CD79b antibody or anti-MHC class II (i.e., anti-CL II) antibody. For example, Figure 23 and Table 7 show that anti-CD22 antibody alone induces no more than about 10% of cells to exhibit pro-apoptotic behavior (solid bar corresponding to "22" in Fig. 23) and anti-CD79b induces about 26% pro-apoptotic cells (solid bar corresponding to "CD79b" in Fig. 23). In combination, however, anti-CD22 and anti CD79b induce only about 16% pro-apoptotic cells (slant-striped bar corresponding to "79b" in Fig. 23). Thus, the combined antibodies induce 16% pro-apoptotic cells, which is less than the 38% sum of the individual effects attributable to anti-CD22 (12%) and anti-CD79b (26%). Using this approach, an inspection of Figure 23 and/or Tables 7-8 reveals that anti-CD22 antibody, and by extension a multispecific, multivalent binding molecule comprising an anti-CD22 binding domain, when used in separate combination with each of the following antibodies (or corresponding binding domains): anti-CD19, anti-CD20, anti-CD21, anti-CD23, anti-CD30, anti-CD37, anti CD40, anti-CD70, anti-CD72, anti-CD79a, anti-CD79b, anti-CD80, anti-CD81, anti CD86 and anti-MHC class II antibodies/binding domains, will result in an inhibited overall effect.

Without wishing to be bound by theory, the data can be interpreted as indicating that anti-CD22 antibody, or a multispecific, multivalent binding molecule comprising an anti-CD22 binding domain, will protect against, or mitigate an effect of, any of the antibodies listed immediately above. More generally, a multispecific, multivalent binding molecule comprising an anti-CD22 binding domain will inhibit the effect arising from interaction with any of CD19, CD20, CD21, CD23, CD30, CD37, CD40, CD70, CD72, CD 79a, CD79b, CD80, CD81, CD86, and MHC class II molecules. It can be seen in Figure 23 and Table 8 that anti-CD22 antibody, and by extension a binding domain comprising an anti-CD22 binding domain, will function as an inhibitor or mitigator of the activity of any antibody/binding domain recognizing a B-cell surface marker such as a CD antigen. Multivalent binding molecules, including multispecific, multivalent binding molecules, are expected to be useful in refining treatment regimens for a variety of diseases wherein the activity of a binding domain needs to be attenuated or controlled.

In addition to the inhibitory, additive or synergistic combined effect of two binding domains interacting with a target cell, typically through the binding of cell surface ligands, the experimental results disclosed herein establish that a given pair of binding domains may provide a different type of combined effect depending on the relative concentrations of the two binding domains, thereby increasing the versatility of the invention. For example, Table 8 discloses that anti-CD21 and anti-CD79b interact in an inhibitory manner at the higher tested concentration of anti-CD79b, but these two antibodies interact in a synergistic manner at the lower tested concentration of anti-CD79b. Although some embodiments will use a single type of multivalent binding molecule, i.e., a monospecific, multivalent binding molecule, comprising, e.g., a single CD21 binding domain and a single CD79b binding domain, the invention comprehends mixtures of multivalent binding molecules that will allow adjustments of relative binding domain concentrations to achieve a desired effect, such as an inhibitory, additive or synergistic effect. Moreover, the methods of the invention encompass use of a single multivalent binding molecule in combination with another binding molecule, such as a conventional antibody molecule, to adjust or optimize the relative concentrations of binding domains. Those of skill in the art will be able to determine useful relative concentrations of binding domains using standard techniques (e.g., by designing experimental matrices of two dilution series, one for each binding domain).

Without wishing to be bound by theory, it is recognized that the binding of one ligand may induce or modulate the surface appearance of a second ligand on the same cell type, or it may alter the surface context of the second ligand so as to alter its sensitivity to binding by a specific binding molecule such as an antibody or a multivalent binding molecule.

Although exemplified herein using B cell lines and antigens, these methods to determine optimally effective multivalent binding molecules (i.e., scorpions) are applicable to other disease settings and target cell populations, including other normal cells, their aberrant cell counterparts including chronically stimulated hematopoietic cells, carcinoma cells and infected cells.

Other signaling phenotypes such as Ca mobilization; tyrosine phosphoregulation; caspase activation; NF-B activation; cytokine, growth factor or chemokine elaboration; or gene expression (e.g., in reporter systems) are also amenable to use in methods of screening for the direct effects of monoclonal antibody combinations.

As an alternative to using a secondary antibody to cross-link the primary antibodies and mimic the multivalent binding molecule or scorpion structure, other molecules that bind the Fc portion of antibodies, including soluble Fc receptors, protein A, complement components including Clq, mannose binding lectin, beads or matrices containing reactive or cross-linking agents, bifunctional chemical cross linking agents, and adsorption to plastic, could be used to cross-link multiple monoclonal antibodies against the same or different antigens.

Example 12

Multivalent Binding Proteinwith Effector Function, or Scorpion, Structures

The general schematic structure of a scorpion polypeptide is H2N-binding domain 1-scorpion linker-constant sub-region-binding domain 2. scorpions may also have a hinge-like region, typically a peptide region derived from an antibody hinge, disposed N-terminal to binding domain 1. In some scorpion embodiments, binding domain 1 and binding domain 2 are each derived from an immunoglobuin binding domain, e.g., derived from a VL and a VH. The VL and a VH are typicallyjoined by a linker. Experiments have been conducted to demonstrate that scorpion polypeptides may have binding domains that differ from an immunoglobulin binding domain, including an Ig binding domain from which the scorpion binding domain was derived, by amino acid sequence differences that result in a sequence divergence of typically less than 5%, and preferably less than 1%, relative to the source Ig binding domain.

Frequently, the sequence differences result in single amino acid changes, such as substitutions. A preferred location for such amino acid changes is in one or more regions of a scorpion binding domain that correspond, or exhibit at least 80% and preferably 85% or 90%, sequence identity to an Ig complementarity determining region (CDR) of an Ig binding domain from which the scorpion binding domain was derived. Further guidance is provided by comparing models of peptides binding the same target, such as CD20. With respect to CD20, epitope mapping has revealed that the 2H7 antibody, which binds CD20, recognizes the Ala-Asn-Pro-Ser (ANPS) motif of CD20 and it is expected that CD20-binding scorpions will also recognize this motif. Amino acid sequence changes that result in the ANPS motif being deeply embedded in a pocket formed of scorpion binding domain regions corresponding to Ig CDRs are expected to be functional binders of CD20. Modeling studies have also revealed that scorpion regions corresponding to CDR3 (VL), CDR1-3 (V) contact CD20 and changes that maintain or facilitate these contacts are expected to yield scorpions that bind CD20.

In addition to facilitating interaction of a scorpion with its target, changes to the sequences of scorpion binding domains (relative to cognate Ig binding domain sequences) that promote interaction between scorpion binding domain regions that correspond to Jg VL and VH domains are contemplated. For example, in a CD20 binding scorpion region corresponding to VL, the sequence SYLV may be changed by substituting an amino acid for Val (V33), such as His, resulting in the sequence SYIH. This change is expected to improve interaction between scorpion regions corresponding to VL and VH domains. Further, it is expected that the addition of a residue at the N-terminus of a scorpion region corresponding to VH-CDR3 will alter the orientation of that scorpion region, likely affecting its binding characteristics, because the N-terminal Ser of VH-CDR3 makes contact with CD20. Routine assays will reveal those orientations that produce desirable changes in binding characteristics, It is also contemplated that mutations in scorpion regions corresponding to VH-CDR2 and/or Vs-CDR3 will create potential new contacts with a target, such as CD20. For example, based on modeling studies, it is expected that substitutions of either Y105 and W106 (found in the sequence NSYW) in a region corresponding to VH-CDR3 will alter the binding characteristics of a scorpion in a manner amenable to routine assay for identifying scorpions with modified binding characteristics. By way of additional example, it is expected that an alteration in the sequence of a scorpion binding domain corresponding to an Ig VL-CDR3, such as the Trp (W) in the sequence CQQW, will affect binding. Typically, alterations in a scorpion region corresponding to an Ig CDR will be screened for those scorpions exhibiting an increase in affinity for the target.

Based on the model structure of the humanized CD20 scFv binding domain 20-4, on the published information relating to the CD20 extracellular loop structure (Du, et al., J Biol. Chem. 282(20):15073-80 (2007)), and on the CD20 binding epitope recognized by the mouse 2H7 antibody (which was the source of CDRs for the humanized 20-4 scFv binding domain), mutations were engineered in the CDR regions of the 2Lm20-4x2Lm20-4 scorpion with the aim of improving the affinity of its binding to CD20. First, the mutations were design to influence the 20-4 CDR conformation and to promote more efficient binding to the CD20 extracellular loop. Second, the introduced changes were designed to provide new intermolecular interactions between the 2Lm20-4x2Lm20-4 scorpion and its target. These mutations include: VL CDR1 V33H i.e., a substitution of His for Val at position 33 of CDR1 in the VL region), VL CDR3 W90Y, VH CDR2 D57E, VH CDR3 insertion of V after residue S99, VH CDR3 Y101K, VH CDR3 N103G, VH CDR3 N104G, and VH CDR3 Y105D. Due to expected synergistic effects of combining some of theses mutations, 11 mutants were designed, combining different mutations as shown in Table 9 (residues introduced by mutation are bolded and underscored).

Table 9

VL CDR1 VL CDR3 VH CDR2 VH CDR3

RASSSVSYIH QQWSFNPPT AIYPGNGDTSYNQKFKG SVYYSNYWYFDL RASSSVSYIH QQWSFNPPT AIYPGNGDTSYNQKFKG SVYYGYWYFDL RASSSVSYIH QQWSFNPPT AIYPGNGDTSYNQKFKG SYYSNSDWYFDL RASSSVSYIH QQWSFNPPT AIYPGNGDTSYNQKFKG SYYSGGDWYFDL RASSSVSYIV QQWSFNPPT AIYPGNGDTSYNQKFKG SYKSNSYWYFDL RASSSVSYIV QQWSFNPPT AIYPGNGETSYNQKFKG SYYSNSYWYFDL RASSSVSYIV QQYSFNPPT AIYPGNGDTSYNQKFKG SYYSNSYWYFDL RASSSVSYIH QQWSFNPPT AIYPGNGDTSYNQKFKG SYKSNSDWYFDL RASSSVSYIH QQWSFNPPT AIYPGNGETSYNQKFKG SYYSNSDWYFDL RASSSVSYIH QQXSFNPPT AIYPGNGDTSYNQKFKG SYYSNSDWYFDL RASSSVSYIH QQYSFNPPT AIYPGNGETSYNQKFKG SYKSGGDWYFDL

Mutations were introduced into binding domains of the CD20xCD20 scorpion (2Lm20-4x2Lm20-4) by PCR mutagenesis using primers encoding the altered sequence region. After sequence confirmation, DNA fragments encoding the 2Lm20 4 scFv fragments with corresponding mutations were cloned into a conventional expression vector containing a coding region for the constant sub-region of a scorpion, resulting in a polynucleotide containing the complete DNA sequence of new versions of the 2Lm20-4x2Lm20-4 scorpion. The variants of the 2Lm20-4x2Lm20-4 scorpion with CDR mutations were produced by expression in a transient COS cell system and purified through Protein A and size-exclusion (SEC) chromatography. The binding properties of 2Lm20-4x2Lm20-4 scorpion variants were evaluated by FACS analysis using primary B-cells and the WIL2-S B-lymphoma cell line.

Other mutants have also been generated using a similar approach to optimize CD20 binding domains. The CD20 SMIP designated TRU015 served as a substrate for generating mutants and, unless noted to the contrary, all domains were human domains. The following mutants were found to contain useful and functional CD20 binding domains. The 018008 molecule contained a substitution of Q (single-letter amino acid code) for S at position 27 of CDR1 in VL, a substitution of S for T at position 28 in CDRi of VH and a substitution of L for V at position 102 in CDR3 of VH. The following partial scorpion linker sequences, corresponding to the CCCP sequence in an IgG1 hinge, were separately combined with the mutated VL and VH: CSCS, SCCS and SCCP, consistent with the modular design of scorpions. The 018009 molecule contained a substitution of Q for S at position 27 of CDRi of VL, a substitution of S for T at position 28 of CDRI of VH and substitutions of S for V at position 96, L for V at position 102 and deletion of the V at position 95, all in CDR3 of VH. The same scorpion linkers sub-sequences described above as being found in the scorpion linkers used in 018008 were used in 018009. The 018010 molecule contained substitutions of a Q for S at position 27, an I for M at position 33 and a V for H at position 34, all in CDR Iof VL, along with an S for T substitution at position 28 of CDR] of VH and an L for V substitution at position 102 in CDR3 of VH. Scorpion linkers defined by the CSCS and SCCS sub-sequences were used with 018010. 018011 contained the same mutations in CDR1 of VL and in CDR1 of VH as described for 018010, along with deletion of V at position 95, substitution of S for V at position 96 and substitution of L for V at postion 102, all in CDR3 of VH. Scorpion linkers defined by the CSCS, SCCS and SCCP sub-sequences were used in 018011 molecules. The 018014 VL was an unmutated mouse VL, with a human VH containing the S for T change at 28 in CDR1 and the L for V change at 102 in CDR3. 018015 also contained an unmutated mouse VL along with a human VH containing an S for T change at 28 of CDR1 and, in CDR3, a deletion of V at 95, substitution of S for V at 96, and substitution of L for V at 102. The 2Lm5 molecule had a Q for S at 27in CDR1 of VL, an F for Y at 27 and an S for T at 30, both in CDR1 of VH, as well as deletion of the V at 95, S for V at 96 and L for V at 102, all in CDR3 of VH. Scorpion linkers defined by the CSCS, SCCS and SCCP were separately used in each of 018014 and 018015. 2Lm5-1 was the same as 2Lm5 except 2Lm5-1 had no mutations in CDR I of VH, and only a scorpion linker defined by the CSSS sub sequence was used. 2Lm6-1 had the mutations of 2Lm5 and a substitution of T for S at 92 and S for F at 93 in CDR3 of VL, and only the scorpion linker defined by the CSSS sub-sequence was used. The only mutations in 2Lm16 were the mutations in CDR3 of VH listed above for 2Lm5-1. Scorpion linkers defined by the sub-sequences CSCS, SCCS, and SCCP were separately used in 2Lm16. 2Lm16-1 substituted Q for

S at 27 in CDR1 of VL and substituted T for S at 92, and S for F at 93, both in CDR3 of VL, and, in CDR3 of VH, deleted V at 95, substituted S for V at 96 and substituted L for V at 102; only the scorpion linker defined by the CSSS sub-sequence was used. 2Lm19-3 substituted Q for S at 27, Ifor M at 33, and V for H at 34, all in CDR1 of VL, along with the mutations in CDR3 of VH listed for 2Lm16-1. Scorpionlinkers defined by the sub-sequences CSCS, SCCS, and SCCP were separately used in 2Lml9-3. The 2Lm20-4 molecule contained an I for M at 33 and a V for H at 34, both in CDR1 of VL, along with the mutations in CDR3 of VH listed for 2Lm16-1. For 2Lm5-1, 2Lm6-1, 2Lm16, 2Lm16-1, 2Lm19-3, and 2Lm20-4, there also was an S for L substitution at position 11 in the framework region of VH. Scorpion linkers defined by the CSCS, SCCS and SCCP sub-sequences were separately used in 2Lm20-4. Finally, the substitution of S for P at position 331 was present in the following mutants: 018008 with the scorpion linker defined by CSCS, 018009 with each of scorpion linkers defined by CSCS and SCCP, 018010 with the scorpion linker defined by CSCS, 018011 with the scorpion linker defined by SCCP, 018014 with the scorpion linker defined by CSCS, 018015 with the scorpion linker defined by CSCS, 2Lml6 with scorpion linkers defined by any of CSCS, SCCS, and SCCP, 2Lm19-3 with a scorpion linker defined by CSCS or SCCP, and 2Lm20-4 with a scorpion linker defined by CSCS or SCCP.

In addition, changes in the length of a linker joining two regions of a binding domain, such as regions of a scorpion binding domain that correspond to an Ig VL and VH, are contemplated. For example, removal of a C-terminal Asp in interdomain linkers where it is found is expected to affect the binding characteristics of a scorpion, as is a substitution of Gly for Asp.

Also contemplated are scorpions that have a scorpion linker (interposed C terminal to the constant sub-region and N-terminal to binding domain 2) that is lengthened relative to a hinge region of an Ig, with amino acid residues being added C-terminal to any cysteine in the scorpion that corresponds to an Ig hinge cysteine, with the scorpion cysteine being capable of forming an interchain disulfide bond. Scorpions containing these features have been constructed and are characterized below.

Efforts were undertaken to improve the expression, stability and therapeutic potency of scorpions through the optimization of the scorpion linker covalently joining the constant sub-region and the C-terminally disposed binding domain 2. The prototypical scorpion used for optimization studies contained an anti-CD20 scFV (binding domain 1) fused N-terminal to the constant sub-region derived from IgG1 CH2 and CH3, with a second anti-CD20 scFv fused C-terminal to that constant sub region. This scorpion, like immunoglobulin molecules, is expected to associate through the constant region (or sub-region) to form a homodimeric complex with peptide chains linked by disulfide bonds. To obtain high level of expression of a stable, tetravalent molecule with high affinity for its CD20 target, the scorpion linker between the constant sub-region and the second binding domain must accommodate the following considerations. First, steric hindrance between the homologous binding domains carried by the two scFv fragments (one scFv fragment on each of two scorpion monomers) should be minimized to facilitate maintenance of the native conformations of each binding domain. Second, the configurations and orientations of binding domains should allow productive association of domains and high-affinity binding of each binding domain to its target. Third, the scorpion linker itself should be relatively protease-resistant and non-immunogenic.

In the exemplary CD20xCD20 scorpion construct SO129, the C-terminus of CH3 and the second anti-CD20 scFV domain were linked by the 2H7 scorpion linker, a peptide derived from, and corresponding to, a fragment of a natural human hinge sequence of IgG1. The 2H7 scorpion linker served as a base for design efforts using computer-assisted modeling that were aimed at improving the expression of scorpions and improving the binding characteristics of the expressed molecules.

To analyze the 2H7 scorpion linker, the 3-dimensional structure of a dimeric form of the human IgG1 hinge was modeled using Insight II software. The crystal structure of anti-CD20 scFV in the VH-VL orientation was chosen as a reference structure for the 20-4 binding domains (RCSB Protein Data Bank entry code: lA14). In intact IgG1, the hinge connects the C-terminus of theCHI domain to the N-terminus of the CH2 domain, with the configuration of each domain being such that hinge cysteine residues can pair to form a homodimer. In the exemplary scorpion molecule, the hinge-derived 2H7 linker connected the C-terminal end of the scorpion domain derived from the IgG1 CH3 domain to the N-terminal end of that portion of scorpion binding domain 2 derived from an IgGI VH2 domain. Using a 3-D modeled structure of the VH-VL scFV, expectations of the optimal distance between the C-terminal ends of the 2H7 linkers was influenced by three considerations. First, hinge stability must be maintained, and stability is aided by dimerization, e.g., homodimerization, which means that the hinge cysteines must be able to pair in the presence of the two folded binding domains. Second, two binding domains, e.g., scFVs, must accommodate the 2H7 linker C-termini without steric interference in order to allow for proper protein folding. Third, the CDRs of each binding domain should be able to face the same direction, as in a native antibody, because each binding domain of the prototypical scorpion can bind adjacent receptors (CD20) on the same cell surface. Given these considerations, the distance between the two N-terminal ends of scFvs is expected to be approximately 28A. The distance between the C-terminal ends of the theoretically designed 2H7 linkers in dimeric scorpion forms is expected to be about 16A. To accommodate the distances expected to be needed for optimizing the performance of a scorpion, the C-terminus of the 2H7 linker was extended by at least 3 amino acids. Such an extension is expected to allow for the formation of disulfide bonds between 2H7 linker cysteine residues, to allow for proper folding of the C-terminal binding domain 2, and to facilitate a correct orientation of the CDRs. In addition, in intact IgG I, due to the presence of the CHI and Vu domains between the hinge and binding domains, the distance between the binding domains carried by the two chains is further increased and is expected to further favor the cross-linking of adjacent receptors on the same cell surface. In view of the considerations described above, a set of linkers with different lengths was designed (Table 10). To minimize immunogenicity, natural residues present at the N-terminal end of the CH2 domain (Ala-Pro-Glu-Leu or APEL) were used to lengthen the 2H7 scorpion linker by sequence addition to the C-terminus of the scorpion linker. The longer constructs contained one or multiple (Gly4Ser) linker units known to be protease-resistant and flexible.

The CD20xCD20 scorpion constructs containing extended scorpion linkers between the CH3 domain of the constant sub-region and the C-terminal scFv binding domain were constructed using PCR mutagenesis and subcloned into a conventional mammalian expression vector. The effect of linker length on CD20xCD20 scorpion expression could be analyzed be comparing the yield of secreted protein in transient expression experiments using COS or HEK293 cells, or by analysis of protein synthesis and accumulation in the cells by Western blot analyses or pulse-chase studies with [35]S-labeled methionine/cysteine.

Table 10

Construct Scorpion Extension sequence Extended scorpion linker Number linker core sequence (2H7) sequence

I GCPPCPNS APEL GCPPCPNSAPEL

2 GCPPCPNS APELGGGGS GCPPCPNSAPELGGGGS

3 GCPPCPNS APELGGGGSGGGGS GCPPCPNS APELGGGGSGGGGS

4 GCPPCPNS APELGGGGSGGGGSGGGGS GCPPCPNS APELGGGGSGGGGSGGGGS

Glycosylated scorpions are also contemplated and, in this context, it is contemplated that host cells expressing a scorpion may be cultured in the presence of a carbohydrate modifier, which is defined herein as a small organic compound, preferably of molecular weight less than 1000 daltons, that inhibits the activity of an enzyme involved in the addition, removal, or modification of sugars that are part of a carbohydrate attached to a polypeptide, such as occurs during N-linked carbohydrate maturation of a protein. Glycosytation is a complex process that takes place in the endoplasmic reticulum ("core glycosylation") and in the Golgi bodies ("terminal glycosylation"). A variety of glycosidase and/or mannosidase inhibitors provide one or more of desired effects of increasing ADCC activity, increasing Fc receptor binding, and altering glycosylation pattern. Exemplary inhibitors include, but are not limited to, castanospermine and kifunensine. The effects of expressing scorpions in the presence of at least one such inhibitor are disclosed in the following example.

Example 13

Scorpionprotein expression levels and characterization

Scorpion protein expression levels were determined and the expressed proteins were characterized to demonstrate that the protein design led to products having practical benefits. A monospecific CD20xCD2O scorpion and a bispecific CD20xCD37 scorpion were expressed in CHO DG44 cells in culture using conventional techniques.

Basal level, stable expression of the CD20xCD2O scorpion SO129 (2m20 4x21m20-4) in CHO DG44 cells cultured in the presence of various feed supplements was observed as shown in Fig. 34. All culture media contained 50 nM methotrexate, a concentration that maintained copy number of the scorpion-encoding polynucleotide. The polynucleotide contained a coding region for the scorpion protein that was not codon-optimized for expression in CHO DG44 cells. The polynucleotide was introduced into cells using the pD18 vector Apparent from Fig, 34, expression levels of about 7-46 ig/ml were obtained.

Expression levels following amplification of the polynucleotide encoding a bispecific CD20xCD37 scorpion were also determined. The pD18 vector was used to clone the CD20xCD37 scorpion coding region and the plasmid was introduced into CHO DG44 cells. Amplification of the encoding polynucleotide was achieved using the dhfr-methotrexate technique known in the art, where increasing concentrations of MTX are used to select for increased copy number of the Dihydrofolate Reductase gene (dhfr), which leads to co-amplification of the tightly linked polynucleotide of interest. Fig. 35 shows that stable expression levels of about 22-118pg/ml of the bispecific CD20xCD37 scorpion were typically observed. Variability in yield was seen under different conditions, including methotrexate concentration used for amplification, but these variables are amenable to optimization by those of skill in the art. A variety of other scorpion molecules described herein were also subjected to expression analyses in CHO and/or COS cells, with the results provided in Table 11, below. These results demonstrate that significant yields of scorpion proteins can be obtained using conventional techniques and routine optimization of the amplification technique.

Expressed proteins were also characterized by SDS-PAGE analysis to assess the degrees of homogeneity and integrity of the expressed proteins and to confirm molecular weight of monomeric peptides. The denaturing polyacrylamide gels (4 20% Tris Glycine) were run under reducing and non-reducing conditions. The results presented in Fig. 36 reveal single protein bands for each of a 2Lm20-4 SCC SMIP and S1000 (CD20(2lm20-4)xCD20(21m20-4) monospecific scorpion. SO126) of the expected monomeric molecular weights under reducing conditions. These data establish that SMIPs and scorpions are amenable to purification in an intact form. Under non-reducing conditions, a trace amount of a peptide consistent with the expected size of a monomeric SMIP was seen, with the vast majority of the protein appearing in a single well-defined band consistent with a dimeric structure. Under these non-reducing conditions, the monospecific scorpion protein showed a single well-defined band of a molecular weight consistent with a dimeric structure. The dimeric structures for both the SMIP and the scorpion are consistent with their monomeric structures, each of which contains a hinge-like scorpion linker containing at least one Cysteine capable of participating in disulfide bond formation.

The effect of scorpion linkers on the expression and integrity of scorpions was also assessed, and results are shown in Table 12. This table lists scorpion linker variants of the monospecific CD20xCD20 (2Lm20-4x2Lm20-4) S0129 scorpion and the CD20xCD28 S0033 scorpion (2H7sccpIgGl-H7-2e12), their integrity as single chain molecules, and their transient expression levels in COS cells relative to the parent scorpion SO129 or S0033, as appropriate, with an H7 linker (set as 100%). Table 13 provides data resulting from an evaluation of scorpion linker variants incorporated into the CD20xCD20 scorpion, along with analogous data for the CD20xCD28 scorpion. Table 13 provides data resulting from an evaluation of SO129 variants containing scorpion linkers that are not hinge-like linkers containing at least one Cysteine capable of disulfide bond formation; rather, the scorpion linkers in these molecules are derived from Type II C-lectin stalks, Apparent from the data presented in Table 13 is that hinge-like scorpion linkers may be associated with scorpions expressed at higher or lower levels than an unmodified parent scorpion linker in transient expression assays. Further, some of the linker variants exhibit greater resistance to proteolytic cleavage than the unmodified parent linker, a concern for all or almost all expressed proteins. The data of Table 13 show that non-hinge-like linkers such as linkers derived from the stalk region of Type II C-lectins are found in scorpions that exhibit binding characteristics that vary slightly from scorpions containing hinge-like scorpion linkers. Additionally, the scorpion containing a non hinge-like scorpion linker exhibits effector function (ADCC) that either equals or exceeds the ADCC associated with scorpions having hinge-like scorpion linkers.

Table I1

tur* Uassam , ea guwrf-m a"s- MWm * As CM=I 'CHO Fi qn; 5Nhrnlartts- aseg baledOf SAAM COE Cfl iar CCl

HM S.2I 4 - I2 174

+ sa estasreK muomsvns -d a no H4T tKSLSLU LSM.FL1---1-2 M2 .CKL~k58">K R!HflERIH!*$ .0 im

+ .- V'.--. LV NW .. -- ~QKS'~LS H56 I3KSLSP 9! ..... RV hII- PI Wd1SL!~ 141- __ ..... N1' 1IR5M11

MAi MW8dSWdNG ....... PKRCGATCN *

H2:1? IK3tsrI NSERPFPPN4S 19 1

a.eege oi&tiedbS0St by.-W'4NtEiar «ccfs. M-Ie':1 and e"isgAor eid(Haso MES goaled~

Table 12

Linker S0129 (2Lm20-4 x 2Lm20-4) Changes Linker 20x20 20x20 Name linker variants - aa seq in CH3?' seq. Expression Cleavage? based on 2 3 H7 GCPPCPNS N H7 100 H8 GSPPSPNS N H7 107 +

H9 GSPPSPNS Y H7 142 H10 EPKSTDKTHTCPPCPNS N IgG1 98 hinge H11 EPKSTDKTHTSPPSPNS N IgG1 126 +

hinge H16 LSVKADFLTPSIGNS N CD80 174 H17 LSVKADFLTPSISCPPCPNS N CD80 + 113 +

H7 H18 LSVLANFSQPEIGNS N CD86 165 +4. H19 LSVLANFSQPEISCPPCPNS N CD86 + 161 +

H7 I I I

H20 LKIQERVSKPKISNS N CD2 115 H21 LKIQERVSKPKISCPPCPNS N CD2 + H7 90 +++ H22 LNVSERPFPPHIQNS N CD22 149 ++ H23 LDVSERPFPPHIQSCPPCPNS N CD22+ 121 ++ ____ ____ H7 H24 REQLAEVTLSLKANS N CDBO 145 ++ H25 REQLAEVTLSLKACPPCPNS N C080+ 98

+ N 70++HZ H26 RIHQMNSELSVLANS N CD86 170

+ H27 RIHQMNSELSVLACPPCPNS N CD86 + 154 ++ H7 H28 DTKGKNVLEKIFSNS N CD2 153

+ H30 LPPETQESQEVTLNS N CD22 78

+ H32 RIHLNVSERPFPPNS N CD22 184 ++ H33 RIHLNVSERPFPPCPPCPNS N CD22+ 74

+ H36 GCPPCPGGGGSNS N H7 110

+ H40 GCPPCPANS Y H7 110

+ H41 GCPPCPANS Y H7 102 H42 GCPPCPNS V H7 99 H44 GGGASCPPCPGNS Y H7 108

+ H45 GGGASCPPCAGNS H7 107 H46 GGGASCPPCANS Y H7 98 H47 LSVKADFLTPSIGNS Y CD80 141 H48 AOFLTPSIGNS N 0080 137 H50 LSVLANFSQPEIGNS Y CD86 21 H51 LSVLANFSQPEIGNS Y C086 110 H52 SQPEIVPISNS Y CD86 95 H53 SQPEIVPISCPPCPNS Y CD86 + 95 H7 H54 SVLANFSQPEISCPPCPNS Y CD86 + 72 +

H55 RIHQMNSELSVLANS Y CD86 118 +

H56 QMNSELSVLANS y CD86 130 H57 VSERPFPPNS Y CD22 118 H58 KPFFTCGSADTCPNS y CD72 103 H59 KPFFTCGSAOTCPNS Y CD72 94

H60 QYNCPGQYTFSMNS Y 00691 H61 EPAFTPGPNIELQKDSDCNS Y CD94 >100 H62 QRHNNSSLNTRTQKARHCNS Y NKG2A >100 H63 NSLFNQEVOIPLTESYCNS Y NKG2D >100

-7'chtangesestothendofdHosuchas1-9 s aetiton and/oroodon optimization 2Transient expression in COS (6W plates), relative to5S0129-H7 parent(% 3Cleavage product(s) observed by SDS-PAGE/silver stain:

-=none, +=faint band, ++=major band(s), +++>50% cleaved bH60-H63 variants compared by estimation of recovery of protein purified from COS spent media.

Table 13

Protein Descriptio Productio % PO1 Improve Binding to ADCC Sequence of s n nYield (M.wt ment Ramos assay scorpion linker (ug in Kd over protein by S0129wt purified/m MALS POI Isup) )

SO129w 117 linker 1.6 67 - - - GCPPC t (167 ) I SO129- CD69 stalk 2.9 66 1.8 Weaker *Slightly QYNCPGQYTF CD69 (167) than better than SM SO129 wt S0129wt POI S0129- CD72 2.0 69 1.2 Similar to *Slightly PFFTCGSADTC CD72 truncated (165) SO129wt better than stalk S0129wt POI SO129- CD94 stalk 2.9 67 1.8 Similar to *Slightly EPAFTPGPNIE CD94 (171) SO129wt better than LQKDSDC SO129wt POI I0129- NKG2A 2.5 93 2.2 Slightly Similar to QRHNNSSLNT NKG2 stalk (170) better than S0129wt RTQKARHC A SO129wt P01 S0129- NKG2D 1.9 70 1.2 Similar to *Slightly NSLFNQEVQIP NKG2 stalk (166) S0129wt better than LTESYC D SOI29wt POI

As noted in the preceding example, production by expression of scorpions in cultures containing a carbohydrate modifier is contemplated. In exemplary embodiments, castanospermine (MW 189.21) is added to the culture medium to a final concentration of about 200 pM (corresponding to about 37.8pg/mL), or concentration ranges greater than about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, or 150 pM, and up to about 300, 275, 250, 225, 200, 175, 150, 125, 100, 75, 60, or 50 tg/mL. For example, ranges of 10-50, or 50-200, or 50-300, or 100-300, or 150-250 pM are contemplated. In other exemplary embodiments, DMJ, for example DMJ-HC (MW 199.6) is added to the culture medium to a final concentration of about 200 pM (corresponding to about 32.6 pg DMJ/mL), or concentration ranges greater than about 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, or 150 pM, and up to about 300, 275, 250, 225, 200, 175, 150, 125, 100, 75, 60, or 50 g/mL. For example, ranges of 10-50, or 50-200, or 50-300, or 100-300, or 150-250 M are contemplated. In other exemplary embodiments, kifunensine (MW 232.2) is added to the culture medium to a final concentration of about 10 pM (corresponding to about 2.3pg/mL), or concentration ranges greater than about 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 pM, and up to about 50, 45, 40, 35, 30, 25, 20, 19, 18, 17, 16, 15, 14, 13, 12, or 11 M. For example, ranges of1-10, or 1-25, or 1 50, or 5-10, or 5-25, or 5-15 pM are contemplated.

In one experiment, a monospecific CD20xCD20 scorpion (S0129) was expressed in cells cultured in 200pM castanospermine (SO129 CS200) or 10 M (excess) kifunensine (S0129 KF 10) and the binding, or staining, of WIL2S cells by the expressed scorpion was measured, as shown in Fig. 42. In comparative binding studies, moreover, a glycosylated SO129 scorpion bound CD16 (FCyRIII) approximately three times better than the unglycosylated SO129 scorpion.

In another study, the ADCC-mediated killing of BJAB B-cells by humanized CD20xCD2Oscorpion (80129) was explored. The results shown in Fig. 43 establish that the scorpion, when expressed in cells being cultured in the presence of either castanospermine or kifunensine, led to significantly more potent ADCC-mediatd BJAB B-cell death for a given concentration of scorpion exposure.

Example 14

Scorpion binding

a. Domain spacing

Bispecific scorpions are capable of binding at least two targets simultaneously, utilizing the pairs of binding domains at the N- and C-terminus of the molecule. In so doing, for cell-surface targets, the composition can cross-link or cause the physical co-approximation of the targets. It will be appreciated by those skilled in the art that many receptor systems are activated upon such cross-linking, resulting in signal induction causing changes in cellular phenotype. The design of the compositions disclosed herein was intended, in part, to maximize such signaling and to control the resultant phenotype.

Approximate dimensions of domains of the scorpion compositions, as well as expectations of interdomain flexibility in terms of ranges of interdomain angles, are known and were considered in designing the scorpion architecture. For scorpions using scFv binding domains for binding domains 1 and 2 (BD1 and BD2), an IgG1 N terminal hinge (H1), and the H7 PIMS linker described herein, the binding domain at the N-terminus and the binding domain at the C-terminus may be maximally about 150-180A apart and minimally about 20-30A apart. Binding domains at the N terminus may be maximally about 90-100A apart and minimally about 10-20A apart (Deisenhofer, et al., 1976, Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 435-445; Gregory, et al., 1987, Mol. Immunol. 24(8):821-9.; Poljak, et al., 1973, Proc. Natl. Acad. Sci., 1973, 70: 3305-3310; Bongini, et al., 2004, Proc. Natl. Acad. Sci. 101: 6466-6471; Kienberger, et al., 2004, EMBO Reports, 5: 579 - 583, each incorporated herein by reference). The choice of these dimensions was done in part to allow for receptor-receptor distances of less than about 50A in receptor complexes bound by the scorpion as distances less than this may be optimal for maximal signaling of certain receptor oligomers (Paar, et al., 2002, J. Immunol., 169: 856-864, incorporated herein by reference) while allowing for the incorporation of Fc structures required for effector function.

The binding domains at the N- and C-terminus of scorpions were designed to be flexible structures to facilitate target binding and to allow for a range of geometries of the bound targets. It will also be appreciated by those skilled in the art that flexibility between the N- or C-terminal binding domains (BD1 and BD2, respectively) and between the binding domains and the Fc domain of the molecule, as well as the maximal and minimal distances between receptors bound by BD1 and/or BD2, can be modified, for example by choice of N-terminal hinge domain (H1) and, by structural analogy, the more C-terminally located scorpion linker domain (H2). For example hinge domains from IgG1,lgG2, IgG3, IgG4, IgE, IgA2, synthetic hinges and the hinge-likeC 2 domain of IgM show different degrees of flexibility, as well as different lengths. Those skilled in the art will understand that the optimal choice of Hi and scorpion linker (H2) will depend upon the receptor system(s) the scorpion is designed to interact with as well as the desired signaling phenotype induced by scorpion binding.

In some embodiments, scorpions have a scorpion linker (H2) that is a hinge like linker corresponding to an Ig hinge, such as an IgGI hinge. These embodiments include scorpions having an amino acid sequence of the scorpion hinge that is an N terminally extended sequence relative to, e.g., the H7 sequence or the wild-type IgGI hinge sequence. Exemplary scorpion linkers of this type would have the sequence of the H7 hinge N-termanally extended by H 2N-APEL(x)y-CO 2H, where x is a unit of the Gly4Ser linker and y is a number between 0 and 3. Exemplifying the influence of the scorpion linker on scorpion stability is a study done using two scorpions, a bispecific CD20xCD28 scorpion and a monospecific CD20xCD2O scorpion. For each of these two scorpion designs, a variety of scorpion linkers were inserted. In particular, scorpion linkers H16 and HI7, which primarily differ in that H17 has the sequence of H16 with the sequence of H7 appended at the C-terminus, and scorpion linkers H18 and 19, in which analogously the sequence of H7 is appended at the C terminus of H18 in generating H19. For each of the two scorpion backbones (20x28 and 20x20), each of the four above-described scorpion linkers were inserted at the appropriate location. Transient expression of these constructs was obtained in COS cells and the scorpion proteins found in the culture supernatants were purified on protein A/G-coated wells (Pierce SEIZE IP kit). Purified proteins were fractionated on SDS-PAGE gels and visualized by silver stain. Inspection of Fig. 44 reveals that the additional H7 sequence in the scorpion linker adds to the stability of each type of scorpion linker and each type of scorpion protein. In other words, appending H7 to the C-terminus of either H16 or H18 added to the stability of the scorpion molecule, and this observation held regardless of whether the scorpion was CD20xCD28 or CD20xCD2O. In terms of target binding, the scorpion proteins having the CD20xCD2O architecture exhibited similar binding properties to the parent monospecific humanized CD20xCD20 scorpion SO129, as shown in Fig. 45.

Beyond the preceding embodiments, however, it may be desirable to prevent bound receptors from approaching within about 50A of each other to intentionally create submaximal signals (Paar, et al., J. Immunol., 169: 856-864). In such a case, choices of Hi and Scorpion linker (H2) that are shorter and less flexible than those described above would be expected to be appropriate.

The same spacing considerations apply to scorpion linkers that are not hinge like. These scorpion linkers are exemplified by the class of peptides having the amino acid sequence of a stalk region of a C-lectin. Exemplary scorpion hinges comprising a C-lectin stalk region are scorpion hinges derived from the CD72 stalk region, the CD94 stalk region, and the NKG2A stalk region. Scorpions containing such scorpion hinges were constructed and characterized in terms of expression, susceptibility to cleavage, and amenability to purification. The data are presented in Table 14.

Table 14

Bench-top Linker G 4S Codon End of SC129 Scorpion Linker variants Linker seq. Expression purification Name optimization' CH3 amino acid seq based on (%SO129) Cleavage' %POI H7 N K GPPCPNS H7 100 70 H60 Y(17) K GCPPCPNS H7 114 - ND H61 Y(15) K GCPPCPNS H7 90 - 66 H62 N G QRHNNSSLNTRTQKARHCPNS NKG2A stalk 129 89 H63 Y(17) G QRHNNSSLNTRTQKARHCPNS NKG2A stalk 100 - 85 H64 Y(15) G QRHNNSSLNTRTQKARHCPNS NKG2A stalk 81 - 83 H65 N G EPAFTPGPNIELQKDSDCPNS CD94 stalk 133 - 66 H66 Y(17) G EPAFTPGPNIELQKDSDCPNS CD94 stalk 200 - 64 H67 Y(15) G EPAFTPGPNIELQKDSDCPNS CD94 stalk 129 - 65 RTRYLQVSQQLQQTNRVLEVTNSSLROOLR H68 N G LKITOLGQSAEDLQGSRRELAQSQEALQVEQ CD72full 110 RAHQAAEGQLQACQADRQKTKETLQSEEQ stalk QRRALEQKLSNMENRLKPFFTCGSADTc 75 'odon optimization of Glyer linker, with (17) or without (1) restriction site 2 Estimate of expression in COS based on recovery of protein in benchtop purification Cleavage product(s) observed by SDS-PAGE/Coomassie Blue stain of purified protein

b. Binding of N- and C-terminal binding domains

Both N- and C-terminaldomains participatein targetcell binding

The target cell binding abilities of a CD20 SMIP (TRU015), a CD37 SMIP (SMIPO16), a combination of CD20 and CD37 SMIPS (TRUO15+SMIPO16), and the CD20xCD37 bispecific scorpion (015x016), were assessed by measuring the capacity of each of these molecules to block the binding of an antibody specifically competing for binding to the relevant target, either CD37 or CD20. The competing antibodies were FITC-labeled monoclonal anti-CD37 antibody or PE-labeled monoclonal anti CD20 antibody, as appropriate. Ramos B-cells provided the targets.

Ramos B-cells at 1.2x107/ml in PBS with 5% mouse sera (#100-113, Gemini Bio-Products, West Sacramento, CA) (staining media) were added to 96-well V bottom plates (25 l/well). The various SMIPs and scorpions were diluted to 75 ig/ml in staining media and 4-fold dilutions were performed to theconcentrations indicated in Fig. 38. The diluted compounds were added to the plated cells in addition to media alone for control wells. The cells were incubated for 10 minutes with the compounds and then FITC anti-CD37 antibody (#186-040, Ancell, Bayport, MN) at 5 g/ml and PE anti-CD20 antibody (#555623, BD Pharmingen, San Jose, CA) at 3 g/ml (neet) were added together to the wells in 25 1 staining media. The cells were incubated on ice in the dark for 45 minutes and then washed 2.5 times with PBS. Cells were fixed with 1% paraformaldehyde (#19943 1 LT, USB Corp, Cleveland, OH ) and then run on a FACs Calibur (BD Biosciences, San Jose, CA). The data were analyzed with Cell Quest software (BD Biosciences, San Jose, CA). The results shown in Fig. 38 establish that all SMIPs, SMIP combinations and scorpions containing a CD20 binding site successfully competed with PE-labeled anti CD 20 antibody for binding to Ramos B-cells (upper panel); all SMIPs, SMIP combinations and scorpions containing a CD37 binding site successfully competed with FITC-labeled anti-CD 37 antibody for binding to Ramos B-cells (lower panel). The bispecific CD20xCD37 scorpion, therefore, was shown to have operable N- and C-terminal binding sites for targets on B-cells.

c. Cell-surface persistence

An investigation of the cell-surface persistence of bound SMIPs and scorpions (monospecific and bispecific) on the surface of B-cells revealed that scorpions 6 exhibited greater cell-surface persistence than SMIPs. Ramos B-cells at 6x10 /ml (3x10 5/well) in staining media (2.5% goat sera, 2.5% mouse sera in PBS) were added to 96-well V-bottom plates. Test reagents were prepared at two-fold the final concentration in staining media by making a 5-fold serial dilution of a 500 nM initial stock and then were added 1:1 to the Ramos B-cells. In addition, media controls were also plated. The cells were incubated in the dark, on ice, for 45 minutes. The plates were then washed 3.5 times with cold PBS. The secondary reagent, FITC goat anti human IgG (#H10501, Caltag/Invitrogen, Carlsbad,CA) was then added at a 1:100 dilution in staining media. The cells were incubated for 30 minutes in the dark, on ice. Cells were then washed 2.5 times by centrifugation with cold PBS, fixed with a 1% paraformaldehyde solution (#19943 1 LT ,USB Corp, Cleveland, OH) and then run on a FACs Calibur (BD Biosciences, San Jose, CA). The data were analyzed with

CellQuest software (BD Biosciences, San Jose, CA). Results of the data analysis are presented in Fig. 37, which shows the binding of several SMIPs, a monospecific CD20xCD20 scorpion and a bispecific CD20xCD37 scorpion to their targets on Ramos B cells.

Two tubes of Ramos B-cells (7x10 5/ml) were incubated for 30 minutes on ice with each of the two compounds being investigated, i.e., a humanized CD20 (2Lm20 4) SMIP and a humanized CD20xCD20 (2Lm20-4x2Lm20-4) scorpion, each at 25 ig/ml in Iscoves media with 10% FBS. At the end of the incubation period, both tubes were washed 3 times by centrifugation. One tube of cells was then plated into 96-well flat-bottom plates at 2x1o5 cells/well in 150 1 of Iscoves media with one plate then going into the 37C incubator and the other plate incubated on ice. The second tube of each set was resuspended in cold PBS with 2% mouse serum and 1% 5 sodium azide (staining media) and plated into a 96-well V-bottom plate at 2x10 cells/well for immediate staining with the secondary antibody, i.e., FITC goat anti human IgG (#H10501, Caltag/Invitrogen, Carlsbad, CA). The secondary antibody was added at a 1:100 final dilution in staining media and the cells were stained on ice, in the dark, for 30 minutes. Cells were then washed 2.5 times with cold PBS, and fixed with 1% paraformaldehyde (#19943 1 LT, USB Corp, Cleveland, OH).

At the time points designated in Fig. 39, samples were harvested from the 96 well flat-bottom plates, incubated at either 37Cor on ice, and placed into 96-well V bottom plates (2x105 cells/well). The cells were washed once with cold staining media, resuspended, and the secondary antibody was added at a final dilution of 1:100 in staining media. These cells were incubated on ice, in the dark, for 30 minutes. The cells were then washed 2.5 times by centrifugation in cold PBS, and subsequently fixed with 1% paraformaldehyde. The samples were run on a FACS Calibur (BD Biosciences, San Jose, CA) and the data was analyzed with CellQuest software (BD Biosciences, San Jose, CA). Results presented in Fig. 39 demonstrate that the binding of a SMIP and a scorpion to the surface of B-cells persists for at least six hours, with the monospecific hu CD20xCD20 (2Lm20-4x2Lm20-4) scorpion persisting to a greater extent than the hu CD20 (2Lm20-4) SMIP.

Example 15

Directcell killing by monospecific and bispecific scorpions

Experiments were conducted to assess the capacity of monospecific and bispecific scorpion molecules to directly kill lymphoma cells, i.e., to kill these cells without involvement of ADCC or CDC. In particular, the Su-DHL-6 and DoHH2 lymphoma cell lines were separately subjected to a monospecific scorpion, i.e., a CD20xCD20 scorpion or a CD37xCD37 scorpion, or to a bispecific CD20xCD37 scorpion.

Cultures of Su-DHL-6, DoHH2, Rec-1, and WSU-NHL lymphoma cells were established using conventional techniques and some of these cultures were then individually exposed to a monospecific CD20 SMIP, a monospecific scorpion (CD20xCD20 or CD37xCD37), or a bispecific scorpion (CD20xCD37 or CDI9xCD37). The exposure of cells to SMIPs or scorpions was conducted under conditions that did not result in cross-linking. The cells remained in contact with the molecules for 96 hours, after which growth was measured by detection of ATP, as would be known in the art. The cell killing attributable to the CD20 SMIP and the CD20xCD20 monospecific scorpion are apparent in Fig.24 and Table 15. The cell killing capacity of the CD37xCD37 monospecific scorpion is apparent from Fig. 25 and Table 15, the ability of the CD20xCD37 bispecific scorpion to kill lymphoma cells is apparent from Fig. 26 and Table 15, and the capacity of the CD19xCD37 bispecific scorpion to kill lymphoma cells is evident from Fig. 27 and Table 15. Data were pooled from three independent experiments and points represent the mean SEM. IC50 values in Table 15 were determined from the curves in Figs. 24, 25, and 26, as noted in the legend to Table 15, and are defined as the concentration resulting in 50% inhibition compared to untreated cultures. The data in the figures and table demonstrate that scorpions are greater than 10-fold more potent in killing these cell lines than the free SMIP using the same binding domains.

Table 15

Cell Line

IC 50 (nM) SU-DHL-6 DoHH2 WSU-NHL

Cell Line

CD20 SMIP* >100 60 NA

CD20xCD2O 0.3 4.0 NA scorpion*

CD37 SMIP** >100 >100 NA

CD37xCD37 10 1.2 NA scorpion**

CD20 SMIP and 6 2 NA CD37 SMIP***

CD20xCD37 0.05 0.05 NA scorpion***

CD19 SMIP and 0.16 NA 0.40 CD37 SMIP****

CD19xCD37 0.005 NA 0.04 scorpion****

*Data derived from Fig. 24. **Data derived from Fig. 25. """Data derived from Fig. 26. **** Data derived from Fig. 27.

Additional experiments with the humanized CD20xCD2O scorpion SO129 were conducted in Su-DHL-4, Su-DHL-6, DoHH2, Rec-1, and WSU-NHL cells. The results are presented in Fig. 46 and Fig. 47. The data provided in these figures extends the findings discussed above in showing that scorpions have the capacity to directly kill a variety of cell lines.

The above findings were extended to other monospecific and bispecific scorpions, with each scorpion demonstrating capacity to directly kill B cells. DoHH2 B-cells were exposed in vitro to the monospecific CD20xCD2O scorpion, a monospecific CD37xCD37 scorpion, or a bispecific CD20xCD37 scorpion. The results presented in Fig. 48 demonstrate that bispecific scorpions have kill curves that are different in form from monospecific scorpions.

Culturing Su-DHL-6 cells in the presence of 70 nM CD20xCD2O scorpion (SO129), CD20xCD37 scorpion, or CD37xCD37 scorpion also led to direct B-cell killing in an in vitro environment (Fig. 49). Consistently, Su-DHL-6 cells exposed to either a bispecific CD19xCD37 scorpion or to Rituxano led to direct cell killing, with the bispecific scorpion exhibiting lethality at lower doses, as revealed in Fig. 50.

Another demonstration of direct cell killing was provided by exposing DHL-4 cells to four independent monospecific scorpions recognizing CD20. Two versions of CD20xCD2Oscorpion were designed to incorporate two 20-4 binding domains (20 4x20-4 and SO129) and the second two incorporate a hybrid of the 011 and 20-4 binding domains. All four of the independently constructed and purified versions of the two CD20xCD2O scorpion designs, (20-4x20-4 and SO129) and hybrid (011 x20-4 and 011x20-4AAsp), efficiently killed the DHL-4 cells in a direct manner. Forthis study, DHL-4 cells were treated in vitro with 1 g/ml of the indicated proteins for 24 hours. Cells were then stained with Annexin V and Propidium Iodide, early and late markers of cell death, respectively, and cell populations were quantified by FACS. The results presented in Fig. 51 establish the direct killing capacity of each of the CD20xCD2Oconstructs as evidenced by increased staining shown in black bars. In addition, the results demonstrate that the hybrid 011x20-4 proteins exhibited a slight increase in direct cell killing relative to 20-4x20-4-based scorpions, despite the fact that each of these scorpions monospecifically recognized CD20. In a separate set of experiments, the dose-response of the four independent scorpion constructs was determined by FACS analysis of Annexin V- and Propidium Iodide-stained cell populations. The results, shown in Fig. 52, demonstrate dose-responsive increases in cell death resulting from treatment of the DHL-4 cells with each of the independent scorpion constructs.

Example 16

Accessoryfunctions mediated by scorpions (ADCC & CDC)

a, Scorpion-dependent cellular cytotoxicity

Experiments were conducted to determine whether scorpions would mediate the killing of BJAB B lymphoma cells. BJAB B lymphoma cells were observed to be killed with CD20 and/or CD37 scorpions.

5 Initially, Ix0 7/ml BJAB B-cells were labeled with 500 Ci/mi Cr sodium chromate (#CJS1, Amersham Biosciences, Piscataway, NJ) for 2 hours at 37°C in Iscoves media with 10% FBS. The "Cr-loaded BJAB B cells were then washed 3 times in RPMI media with 10% FBS and resuspended at 4x105/ml in RPML Peripheral blood mononuclear cells (PBMC) from in-house donors were isolated from heparinized whole blood via centrifugation over Lymphocyte Separation Medium (#50494, MP Biomedicals, Aurora, Oh), washed 2 times with RPMI media and resuspended at 5x10 6/ml in RPMI with 10% FBS, Reagent samples were added to RPMI media with 10% FBS at 4 times the final concentration and three 10-fold serial dilutions for each reagent were prepared. These reagents were then added to 96-well U- bottom plates at 50 pl/well to the indicated final concentrations. The "Cr-labeled BJAB were then added to the plates at 50 pl/well (2x10 /well). The PBMC were then 4

added to the plates at 100 pl/well (5x105/well) for a final ratio of 25:1 effectors (PBMC):target (BJAB). Effectors and targets were added to media alone to measure background killing. The "Cr-labeled BJAB were added to media alone to measure spontaneous release of "Cr and to media with 5% NP40 (#28324, Pierce, Rockford, Ill) to measure maximal release of "'Cr. The plates were incubated for 6 hours at 37°C in 5%CO 2 . Fifty p (25 1 would also be suitable) of the supernatant from each well were then transferred to a LumaPlate-96 (#6006633, Perkin Elmer, Boston, Mass) and dried overnight at room temperature.

After drying, radioactive emissions were quantitated as cpm on a Packard TopCount-NXT. Sample values were the mean of triplicate samples. Percent specific killing was calculated using the following equation: % Kill= ((sample - spontaneous release)/(maximal release - spontaneous release)) x 100. The plots in Fig. 30 show that BJAB B cells were killed by monospecific scorpions CD20xCD20 and CD37xCD37. The combination of CD20 SMIP and CD37 SMIP also killed BJAB B cells. These results demonstrate that scorpions exhibit scorpion-dependent cellular cytotoxicity and it is expected that this functionality is provided by the constant sub region of the scorpion, providing ADCC activity.

b. Scorpion role in complement-dependent cytotoxicity

Experiments also demonstrated that scorpions have Complement-Dependent Cytotoxicity (CDC) activity. The experiment involved exposure of Ramos B-cells to CD19 and/or CD37 SMIPs and scorpions, as described below and as shown in Fig. 31.

The experiment was initiated by adding from 5 to 2.5 x 105 Ramos B-cells to wells of 96-well V-bottomed plates in 50 gl of Iscoves media (no FBS). The test compounds in Iscoves, (or Iscoves alone) were added to the wells in 50 pl at twice the indicated final concentration. The cells and reagents were incubated for 45 minutes at 37 0C. The cells were washed 2.5 times in Iscoves with no FBS and resuspended in Iscoves with human serum (# Al 13, Quidel, San Diego, CA) in 96-well plates at the indicated concentrations. The cells were then incubated for 90 minutes at 37°C. The cells were washed by centrifugation and resuspended in 125 . cold PBS. Cells were then transferred to FACs cluster tubes (#4410, CoStar, Coming, NY) and 125 g PBS with propidium iodide (# P-16063, Molecular Probes, Eugene, OR) at 5 gg/m was added. The cells were incubated with the propidium iodide for 15 minutes at room temperature in the dark and then placed on ice, quantitated, and analyzed on a FACsCalibur with CellQuest software (Becton Dickinson). The results presented in Fig. 31 establish that the CD19 SMIP, but not the CD37 SMIP, exhibits CDC activity, with a combination of the two SMIPs exhibiting approximately the same level of CDC activity as CD19 SMIP alone. The CD19xCD37 scorpion, however, exhibited significantly greater CDC activity than either SMIP alone or in combination, establishing that the scorpion architecture provides a greater level of Complement dependent Cytotoxicity than other molecular designs.

c. ADCC/CDC activity of CD20xCD20 monosnecific scorpions

Three distinct CD20xCD20 monospecific scorpions were examined for ADCC and CDC functionality, along with appropriate controls. ADCC was assayed using conventional techniques, and the results are presented in Fig. 53. Apparent from the

Figure is the appreciable, but not identical, ADCC activity associated with each of the tested CD20xCD20 monospecific scorpions.

To assess CDC, Ramos B-cell samples (4x10') were incubated with each of the CD20xCD20 scorpions (0, 0.5, 5, 50 and 500 nM) and serum (10%) for 3.5 hour at 37°C. Cell death was assessed by 7-AAD staining and FACS analysis. The results are presented in Fig. 54, which reveals that the scorpions exhibit some CDC activity. In a similar experiment, Ramos B-cell samples (4x10 5) were incubated with CD20xCD20 scorpion protein ( 5, 50, 100 nM) and serum (10%) for 2 hour at 37°C. Cells were washed 2X and incubated with anti-human Clq FITC antibody. Bound Clq was assessed by FACS analysis and the results are presented in Fig. 55. These results are consistent with the results presented in Fig. 54 that each of the CD20xCD20 monospecific scorpions was associated with some CDC activity, although less activity than was associated with a CD20 SMIP.

d. Interactions of scorpions with FeyRIII

ELISA studies showed that scorpions bound to FcyRII (CD16) low (a low affinity isoform or allelotype) at increased levels in the absence of target cells. ELISA plates were initially coated with either low- or high-affinity CD16mIgG using conventional techniques. The ability of this immobilized fusion protein to capture either a CD20 SMIP or a CD20xCD20 monospecific scorpion was assessed. Bound SMIPs and scorpions were detected with goat anti-human IgG (HRP) secondary antibody and mean fluorescence intensity (MFI) was determined. PBS alone (negative control) is shown as a single point. The results are presented in Fig. 32A (capture by CD16 high affinity isoform fusion) and 32B (capture by CD16 low affinity isoform fusion). Apparent from a consideration of Figs. 32A and 32B is that both CD20 SMIP and CD20xCD20 monospecific scorpion showed increased binding to both the high- and low-affinity CD16 isoform fusions, with the CD20xCD20 scorpion showing a dramatic increase in binding to the low affinity isoform fusion with increasing protein concentration.

The binding of scorpions to the FcyRIII isoforms in the presence of target cells was also assessed. The data show the increased binding of scorpions to both FcyRIII (CD16) low- and high-affinity isoforms or allelotypes in the presence of target cells with increasing protein concentration.

In conducting the experiment, CD20-positive target cells were exposed to CD20 SMIPs or CD20xCD20 monospecific scorpions under conditions that allowed the binding of the SMIP or scorpion to the CD20-positive target cell. Subsequently, the SMIP- or scorpion-bearing target cell was exposed to either CD16 high- or low affinity isoform tagged with mouse IgFc. A labeled goat anti-mouse Fc was then added as a secondary antibody to label the immobilized CD16 tagged with the mouse IgFc. Cells were then detected using flow cytometry on a FACs Calibur (BD Biosciences, San Jose, CA) and analyzed with Cell Quest software (BD Biosciences, San Jose, CA). As shown in Fig. 33, increased concentrations of each of the CD20 SMIP and the CD20xCD20 monospecific scorpion led to increased binding to the CD16 isoforms in the presence of target cells, with the increase in binding of the CD20xCD20 scorpion being more significant than the increased binding seen with the CD20 SMIP.

Example 17

Cell-cycle effects ofscorpions on target lymphoma cells

The cell-cycle effects of scorpions were assessed by exposing lymphoma cells to SMIPs, monospecific scorpions and bispecific scorpions. More particularly, DoHH2 lymphoma cells (0.5 x 106) were treated for 24 hours with 0.4 nM rituximab, CD20xCD37 scorpion, TRU-015 (CD20 SMIP)+ SMIP-016 combination (0.2 nM each), 100 nM SMIP-016 or 100 nM CD37xCD37 scorpion. These concentrations respresent about 10-fold more than the IC50 value of the scorpion in a 96-hour growth inhibition assay (see Figs. 24-27). Cultures were labeled for 20 minutes at 37C with 10 pM BrdU (bromodeoxyuridine). Following fixation, cells were stained with anti BrdU-FITC antibody and counterstained with propidium iodide. Values in Fig. 28 are the mean +/- SD of 4 replicate cultures from 2-3 independent experiments. All sample data were analyzed at the same time and pooled for presentation using both the BrdU and PI incorporation dot plots. Plots demonstrate that a major effect of scorpion treatment is a depletion of cells in S-phase, as well as an increase in the Go/Gi compartment.

Example 18

Physiologicaleffects ofscorpions

a. Mitochondrial potential

CD20xCD20 scorpions induced loss of mitochondrial membrane potential in DHL4 B-cells, as revealed in a JC-i assay. JC-1 is a cationic carbocyanine dye that exhibits potential-dependent accumulation in the mitochondria (Mitoprobe JC-1 Assay Kit for Flow Cytometry from Molecular Probes). JC-1 is more specific to the mitochondrial membrane than the plasma membrane and is used to determine changes in mitochondrial membrane potential. Accumulation in mitochondria is indicated by a fluorescence shift from green (529nm) to red (590nm).

In conducting the experiment, DHL-4 B-cells (5x105 cells/ml) were initially cultured in 24-well plates and treated for 24 hours with I g/ml CD20xCD20 scorpion, Rituximab, IgG control antibody, or 5 M staurosporine at 37°C, 5%CO2 , in a standard tissue-culture incubator. JC-I dye (10 1/ml, 2 tM final concentration) was added and cells were incubated for another 30 minutes at 37°C. Cells were harvested by centrifugation minutess at 1200 rpm), washed with Iml PBS, and resuspended in 500 1 PBS. Cells were analyzed by flow cytometry (FACSCalibur, BD) with 488 nM excitation and 530 nM and 585 nM emission filters. For the representative scatter plots shown in Fig. 56, red fluorescence was measured on the Y-axis and green fluorescence was measured on the X-axis. Depolarization of the mitochondrial membrane was measured as a decrease in red fluorescence, as seen in the positive control CCCP (carbonyl cyanide 3-chlorophenylhydrazone), a known mitochondrial membrane potential disrupter. To confirm that JC-1 was responsive to changes in membrane potential, DHL-4 B-cells were treated with two concentrations of CCCP (50pM and 250pM) for 5 minutes at 37°C, 5%C0 2 . An additional positive control was cells treated with the broad-spectrum kinase inhibitor staurosporine to induce apoptosis. The results shown in Fig. 56 are dot-plot graphs of 10,000 counts, with red fluorescence plotted on the Y-axis and green fluorescence plotted on the X axis. A summary histogram of the percentage of cells with disrupted mitochondrial membrane potential (disrupted MMP: black bars) is shown in Fig 56. These results demonstrate that treatment with either the 20-4x20-4 scorpion or the 011x20-4 scorpion generated a decrease in the mitochondrial membrane potential associated with cell death.

b. Calcium flux

Scorpion molecules were analyzed for influences on cell signaling pathways, using Ca** mobilization, a common feature of cell signaling, as a measure therefor. SU-DHL-6 lymphoma cells were labeled with Calcium 4 dye and treated with the test molecules identified below. Cells were read for 20 seconds to determine background fluorescence, and then SMIPs/scorpions were added (first dashed line in Fig. 28) and fluorescence was measured out to 600 seconds. At 600 seconds, an 8-fold excess of cross-linked goat-anti-human F(ab)'2 was added and fluorescence was measured for a further 300 seconds. Panel (A) of Fig. 28 shows the results obtained with a combination of CD20 SMIP and CD37 SMIP (red line); or obtained with a CD20xCD37 bispecific scorpion (black line), compared with unstimulated cells (blue line). In panel B of Fig. 28, the results of treating cells withCD20 SMIP alone (red line) resulted in Ca+* mobilization, but this was not as robust as the signal generated by the monospecific CD20xCD20 scorpion (black line). The Ca+* mobilization plots of Fig. 28 represent the fluorescence from triplicate wells treated with equimolar amounts of scorpion and SMIP/SMIP combinations.

c. Caspases 3, 7 and 9

The ability of CD20-binding scorpions to directly kill B-cells as evidenced by increased Annexin V and Propidium Iodide staining and the loss of mitochondrial membrane potential led to an further investigation of additional apoptosis-related effects of CD20-binding scorpions in B-cells. The approach taken was to perform Apo I assays on DHL-4 B-cells exposed to CD20xCD20 scorpions or appropriate controls. The Apol assay is based on a synthetic peptide substrate for caspase 3 and 7. The assay components are available from Promega (Apo-ONE Homogeneuous Caspase-3/7 Assay). Caspase-mediated cleavage of the labeled peptide Z-DEVD Rhodamine 110 releases the fluorescent rhodamine 110 label, which is measured using 485 nm excitation and 530nm detection.

In the experiment, 100 pl DHL-4 B-cells (Ix106 cells/ml) were plated in black 96-well flat-bottom tissue culture plates and treated for 24 or 48 hours with 1 g/ml

CD20xCD20 scorpion, Rituximab, an IgG control antibody, or 5 M staurosporine at 37°C, 5%CO2 in a standard tissue-culture incubator. (Staurosporine is a small molecule, broad-spectrum protein kinase inhibitor that is known in the art as a potent inducer of classical apoptosis in a wide variety of cell types.) After 24 or 48 hours, 100 gl of the 100-fold diluted substrate was added to each well, gently mixed for one minute on a plate shaker (300 rpm) and incubated at room temperature for two hours. Fluorescence was measured using 485 nM excitation and 527 nM emission filter (Fluoroskan Ascent FL, Thermo Labsystems). Graphs showing average fluorescent intensity of triplicate treatments plus/minus standard deviation after 24 hours and 48 hours (24 hours only for staurosporine) are presented in Fig. 57. These results establish that CD20-binding scorpions do not directly kill B-cells by an apoptotic pathway involving activation of caspase 3/7.

The results obtained in the Apo-1 assay were confirmed by Western blot analyses designed to detect pro-caspase cleavage resulting in activated caspase or to detect cleavage of PARP (Poly (ADP-Ribose) Polymerase), a protein known to be cleaved by activated caspase 3. DHL-4 B-cells were exposed to a CD20 binding scorpion or a control for 4, 24, or 48 hours and cell lysates were fractionated on SDS PAGE and blotted for Western analyses using conventional techniques. Fig. 58 presents the results in the form of a collection of Western blots. The bottom three Westerns utilized anti-caspase antibodies to detect shifts in molecular weight of the caspase enzyme, reflecting proteolytic activation. For caspases 3, 7, and 9, there was no evidence of caspase activation by any of the CD20-binding molecules. Staurosporine served as a positive control for the assay, and induced pro-caspase cleavage to active caspase for each of caspases 3, 7 and 9. The fourth Western blot shown in Fig. 58 reveals that PARP, a known substrate of activated caspase 3, was not cleaved, consistent with a failure of CD20-binding scorpions to activate caspase 3. The results of all of these experiments are consistent in showing that caspase 3 activation is not a significant feature of the direct cell killing of DHL-4 B-cells induced by CD20 binding scorpions.

In addition, a time series study was conducted to determine the effect of CD20 binding proteins, including a CD20xCD2O scorpion, on Caspase 3. DoHH2 or Su DHL-6 B-cells were incubated with OnM CD20 binding protein (SO129 scorpion,

2Lm20-4 SMIP, or Rituxan*) +/- soluble CD16 Ig (40nM), soluble CD16 Ig alone, or media. The cells were cultured in complete RPMI with 10% FBS at 3x105/well/300

[d and harvested at 4 hours, 24 hours or 72 hours. The 72-hour time-point samples were plated in 500 pl of the test agent. Cells were washed with PBS and then stained for intracellular active caspase-3 using the BD Pharmingen Caspase 3, Active Form, mAB Apoptosis Kit:FITC (cat no.55048, BD Pharmingen, San Jose, CA). Briefly, after 2 additional washes in cold PBS, the cells were suspended in cold cytofix/cytoperm solution and incubated on ice for 20 minutes. Cells were then washed by centrifugation, aspirated, and washed two times with Perm/Wash buffer at room temperature. The samples were then stained with 20 1 FITC-anti-caspase 3 in 100 R of Perm-Wash buffer at room temperature in the dark for thirty minutes. The samples were then washed two times with Perm-Wash buffer, and resuspended in 500 .1of Perm-Wash buffer. Washed cells were then transferred to FACs tubes and run on a FACs Calibur (BD Biosciences, San Jose, CA) and analyzed with Cell Quest software (BD Biosciences, San Jose, CA). The results are shown in Table 16.

Table 16

Molecule Percentage Caspase-3 positive Percentage in live gate (10 nM) cells

4 hours 24 hours 48 hours 4 hours 24 hours 48 hours

RTXN 7 25 7 75 53 56

and CD16 hi 27 47 21 79 60 43 (4X)

CD20 SMIP 5 5 10 89 85 81 (2Lm20-4)

and CD16 hi 28 54 21 61 60 41

Humanized 7 13 14 69 68 61 CD20xCD20 scorpion

(S00129)

andCD16hi 30 31 15 67 75 72

Media 7 5 9 89 82 80

and CD16 hi 6 5 9 91 83 80

The results of all of these experiments are consistent in showing that there is limited activation of caspase 3 in the absence of CD16, which does not implicate caspase 3 activation as a significant feature of the direct cell killing induced by CD20 binding scorpions.

d. DNA fragmentation

Induction of classical apoptotic signaling pathways ultimately results in condensation and fragmented degradation of chromosomal DNA. To determine whether CD20-binding scorpions directly killed B-cells through a classical apoptotic mechanism, the state of B-cell chromosomal DNA was examined following exposure of the cells to CD20-binding scorpions, or controls. Initially, DHL-4 B-cells were treated in vitro for 4, 24 or 48 hours with a CD20-binding molecule, i.e., the monospecific CD2OxCD20 (2Lm20-4x2Lm20-4) scorpion, the CD20xCD20 (011x2Lm20-4) scorpion, or Rituximab, or with a control. Subsequently, cells were lysed and chromosomal DNA was purified using conventional techniques. The chromosomal DNA was then size-fractionated by gel electrophoresis. The gel electrophoretogram shown in Fig. 59 reveals a lack of DNA fragmentation that demonstrated that the cell death generated by CD20-binding scorpions was not mediated by a classical apoptotic pathway. Staurosporine-treated cells were used as positive control in these assays.

e. SYK phosphorylation

SYK is a phospho-regulated protein with several phosphorylation sites that functions as a transcriptional repressor. SYK is localized to the cell nucleus, but is capable of rapid relocation to the membrane upon activation. For activation, SYK must retain its nuclear localization sequence. Activated SYK has a role in suppressing breast cancer tumors and SYK is activated by pro-apoptotic signals such as ionizing radiation, BCR ligation and MHC class II cross-linking. Further, SYK has been shown to affect the PLC-y and Ca** pathways. Given these observations, the capacity of CD20-binding scorpions to affect SYK was investigated.

DHL-6 B-cells were exposed to a bispecific CD20xCD37 scorpion for 0, 5, 7 or 15 hours and the cells were lysed. Lysates were immunoprecipitated with either an anti-phosphotyrosine antibody or with an anti-SYK antibody. Immunoprecipitates were fractionated by gel electrophoresis and the results are shown in Fig. 60. Apparent from an inspection of Fig. 60 is the failure of the bispecific CD20xCD37 scorpion to induce phosphorylation of SYK, thereby activating it. Consistent with the above-described studies on caspase activation and chromosomal DNA fragmentation, it does not appear that CD20-binding scorpions directly kill B-cells using a classic apoptotic pathway, such as the caspase-dependent pathway. While not wishing to be bound by theory, it is expected that the CD20-binding scorpions directly kill B-cells through a caspase-, and SYK-, independent pathway that does not prominently feature chromosomal DNA fragmentation, at least not on the same time frame as fragmentation occurs during caspase-dependent apoptosis.

Example 19

Scorpion applications

a. In vivo activity of scorpions

The activity of scorpions was also assessed using a mouse model. Measurements of scorpion activity in vivo involved administration of 10-300 tg scorpion and subsequent time-series determinations of serum concentrations of that scorpion. Results of these studies, presented as serum concentration curves for each of two bispecific scorpions (i.e., S0033, a CD20xCD27 scorpion and a CD20xCD37 scorpion) from three-week pharmacokinetic studies in mice are presented in Fig. 40. The data in Fig. 40 show that it took at least 500 hours after administration before the serum levels of each of the two bispecific scorpions fell back to baseline levels. Thus, scorpions show serum stability and reproducible, sustained circulating half-lives in vivo.

The in vivo efficacy of scorpions was also assessed. An aggressive Ramos xenograft model was used in parallel experiments with SMIPs versus historical immunoglobulin controls. The survival curves provided in Fig. 41 reveal that administration of 10 g bispecific scorpion had negligible influence on survival, but administration of 100-300 g had significant positive effect on the survival of mice bearing Ramos xenografts.

b. Combination theories

it is contemplated that scorpions will find application in the prevention, treatment or amelioration of a symptom of, a wide variety of conditions affecting man, other mammals and other organisms. For example, CD20-binding scorpions are expected to be useful in treating or preventing a variety of diseases associated with excessive or aberrant B-cells. In fact, any disease amenable to a treatment involving the depletion of B-cells would be amenable to treatment with a CD20-binding scorpion. In addition, scorpions, e.g., CD20-binding scorpions, may be used in combination therapies with other therapeutics. To illustrate the feasibility of a wide variety of combination therapies, the monospecific CD20xCD20 scorpion (SO129) was administered to Su-DHL-6 B-cells in combination with doxorubicin, vincristine or rapamycin. Doxorubicin is a topoisomerase II poison that interferes with DNA biochemistry and belongs to a class of drugs contemplated for anti-cancer treatment. Rapamycin (Sirolimus) is a macrolide antibiotic that inhibits the initiation of protein synthesis and suppresses the immune system, finding application in organ transplantation and as an anti-proliferative used with coronary stents to inhibit or prevent restenosis. Vincristine is a vinca alkaloid that inhibits tubule formation and has been used to treat cancer.

The experimental results shown in Fig. 61 are presented as Combination Index values for each combination over a range of effect levels. The interactions of the monospecific CD20xCD20 scorpion SO129 are different for each drug class, while with Rituxan* (RTXN) the plots forms are similar. The effect seen with doxorubicin at high concentrations may reflect a shift towards monovalent binding. The data establish that CD20-binding scorpions may be used in combination with a variety of other therapeutics and such combinations would be apparent to one of skill in the art in view of the present disclosure.

Variations on the structural themes for multivalent binding molecules with effector function, or scorpions, will be apparent to those of skill in the art upon review of the present disclosure, and such variant structures are within the scope of the invention.

The entire disclosure in the complete specification of our Australian Patent No. 2007257692, Australian Patent No.2014200661, Australian Patent Application No. 2016231617 and Australian Patent Application No. 2018267609, are by these cross references incorporated into the present application.

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The invention also includes the following items:

1. A multivalent single-chain binding protein with effector function comprising

a. a first binding domain derived from an immunoglobulin or immunoglobulin-like molecule;

b. a constant sub-region providing an effector function, the immunoglobulin constant sub-region located C-terminal to the first binding domain;

c. a scorpion linker located C-terminal to the constant sub-region; and

d. a second binding domain derived from an immunoglobulin or immunoglobulin-like molecule located C-terminal to the constant sub-region;

thereby localizing the constant sub-region between the first binding domain and the second binding domain.

2. The protein according to item 1 wherein the first binding domain comprises a variable light region derived from a first immunoglobulin and a variable heavy region derived from a second immunoglobulin.

3. The protein according to item 2 wherein the first immunoglobulin and the second immunoglobulin are the same immunoglobulin.

4. The protein according to item 1 wherein the second binding domain comprises a variable light region derived from a first immunoglobulin and a variable heavy region derived from a second immunoglobulin.

5. The protein according to item 4 wherein the first immunoglobulin and the second immunoglobulin are the same immunoglobulin.

6. The protein according to item 1 wherein the first binding domain and the second binding domain recognize the same molecular target.

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7. The protein according to item 1 wherein the first binding domain and the second binding domain recognize the same epitope.

8. The protein according to item 1 wherein the first binding domain and the second binding domain recognize different molecular targets on the same eukaryotic cell, prokaryotic cell, virus, carrier, or object.

9. The protein according to item 1 wherein the first binding domain and the second binding domain recognize molecular targets associated with physically distinct eukaryotic cells, prokaryotic cells, viruses, carriers or objects.

10. The protein according to item 1 wherein at least one of the first binding domain and the second binding domain recognize at least one molecular target not associated with a eukaryotic cell, prokaryotic cell, virus, carrier or object.

11. The protein according to item 1 wherein the first binding domain, the second binding domain and the constant sub-region are each derived from a human immunoglobulin.

12. The protein according to item 1 wherein at least one of the first binding domain and the second binding domain recognizes a target on a cancer cell.

13. The protein according to item 11 wherein at least one of the first binding domain and the second binding domain recognizes a target selected from the group consisting of a tumor antigen, a B-cell target, a TNF receptor superfamily member, a Hedgehog family member, a receptor tyrosine kinase, a proteoglycan-related molecule, a TGF-beta superfamily member, a Wnt-related molecule, a receptor ligand, a T-cell target, a Dendritic cell target, an NK cell target, a monocyte/macrophage cell target and an angiogenesis target.

14. The protein according to item 13 wherein the tumor antigen is selected from the group consisting of SQUAMOUS CELL CARCINOMA ANTIGEN 1, SQUAMOUS CELL CARCINOMA ANTIGEN 2, Ovarian carcinoma antigen CA125, MUCIN 1, CTCL tumor antigen sel-1, CTCL tumor antigen se14-3, CTCL tumor antigen se20-4, CTCL tumor antigen se20-9, CTCL tumor antigen se33-1, CTCL tumor

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antigen se37-2, CTCL tumor antigen se57-1, CTCL tumor antigen se89-1, Prostate specific membrane antigen, 5T4 oncofetal trophoblast glycoprotein, Orf73 Kaposi's sarcoma-associated herpesvirus, MAGE-C1, MAGE-B1 ANTIGEN, MAGE-B2 ANTIGEN, MAGE-2 ANTIGEN, MAGE-4a antigen, MAGE-4b antigen, Colon cancer antigen NY-CO-45, Lung cancer antigen NY-LU-12 variant A, Cancer associated surface antigen, Adenocarcinoma antigen ARTI, Paraneoplastic associated brain-testis cancer antigen, Neuro-oncological ventral antigen 2, Hepatocellular carcinoma antigen gene 520, TUMOR-ASSOCIATED ANTIGEN CO-029, Tumor-associated antigen MAGE-X2, Synovial sarcoma X breakpoint 2, Squamous cell carcinoma antigen recognized by T cell, Serologically defined colon cancer antigen 1, Serologically defined breast cancer antigen NY-BR-15, Serologically defined breast cancer antigen NY-BR-16, Chromogranin A; parathyroid secretory protein 1, DUPAN-2, CA 19-9, CA 72-4, CA 195 and L6.

15. The protein according to item 13 wherein the B cell target is selected from the group consisting of CD10, CD19, CD20, CD21, CD22, CD23, CD24, CD37, CD38, CD39, CD40, CD72, CD73, CD74, CDw75, CDw76, CD77, CD78, CD79a/b, CD80, CD81, CD82, CD83, CD84, CD85, CD86, CD89, CD98, CD126, CD127, CDw130, CD138 and CDw150.

16. The protein according to item 13 wherein the TNF receptor superfamily member is selected from the group consisting of 4-1BB/TNFRSF9, NGF R/TNFRSF16, BAFF R/TNFRSF13C, Osteoprotegerin/TNFRSF11B, BCMA/TNFRSF17, OX40/TNFRSF4, CD27/TNFRSF7, RANK/TNFRSF11A, CD30/TNFRSF8, RELT/TNFRSF19L, CD40/TNFRSF5, TACI/TNFRSF13B, DcR3/TNFRSF6B, TNF RI/TNFRSF1A, DcTRAIL RI/TNFRSF23, TNF RII/TNFRSF1B, DcTRAIL R2/TNFRSF22, TRAIL R1/TNFRSF1OA, DR3/TNFRSF25, TRAIL R2/TNFRSF1OB, DR6/TNFRSF21, TRAIL R3/TNFRSF1OC, EDAR, TRAIL R4/TNFRSF1OD, Fas/TNFRSF6, TROY/TNFRSF19, GITR/TNFRSF18, TWEAK R/TNFRSF12, HVEM/TNFRSF14, XEDAR, Lymphotoxin beta R/TNFRSF3, 4-1BB Ligand/TNFSF9, Lymphotoxin, APRIL/TNFSF13, Lymphotoxin beta/TNFSF3, BAFF/TNFSF13C, OX40 Ligand/TNFSF4, CD27 Ligand/TNFSF7, TL1A/TNFSF15, CD30 Ligand/TNFSF8, TNF-alpha/TNFSF1A, CD40 Ligand/TNFSF5, TNF-beta/TNFSFB,

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EDA-A2, TRAIL/TNFSF10, Fas Ligand/TNFSF6, TRANCE/TNFSF11, GITR Ligand/TNFSF18, TWEAK/TNFSF12 and LIGHT/TNFSF14.

17. The protein according to item 13 wherein the is Hedgehog family member is selected from the group consisting of Patched and Smoothened.

18. The protein according to item 13 wherein the receptor tyrosine kinase is selected from the group consisting of Axl, FGF R4, Clq R1/CD93, FGF R5, DDR1, Flt-3, DDR2, HGF R, Dtk, IGF-I R, EGF R, IGF-II R, Eph, INSRR, EphAl, Insulin R/CD220, EphA2, M-CSF R, EphA3, Mer, EphA4, MSP R/Ron, EphA5, MuSK, EphA6, PDGF R alpha, EphA7, PDGF R beta, EphA8, Ret, EphB1, RORi, EphB2, ROR2, EphB3, SCF R/c-kit, EphB4, Tie-i, EphB6, Tie-2, ErbB2, TrkA, ErbB3, TrkB, ErbB4, TrkC, FGF RI, VEGF R1/Flt-1, FGF R2, VEGF R2/Flk-1, FGF R3 and VEGF R3/Flt-4.

19. The protein according to item 13 wherein the proteoglycan-related molecule is selected from the group consisting of proteoglycans and regulators thereof.

20. The protein according to item 13 wherein the Transforming Growth Factor (TGF)-beta superfamily member is selected from the group consisting of Activin RIA/ALK-2, GFR alpha-1, Activin RIB/ALK-4, GFR alpha-2, Activin RIIA, GFR alpha-3, Activin RIIB, GFR alpha-4, ALK-1, MIS RII, ALK-7, Ret, BMPR-IA/ALK-3, TGF-beta RI/ALK-5, BMPR-IB/ALK-6, TGF-beta RII, BMPR-II, TGF-beta RIub, Endoglin/CD105 and TGF-beta RIII.

21. The protein according to item 13 wherein the Wnt-related molecule is selected from the group consisting of Frizzled-1, Frizzled-8, Frizzled-2, Frizzled-9, Frizzled-3, sFRP-1, Frizzled-4, sFRP-2, Frizzled-5, sFRP-3, Frizzled-6, sFRP-4, Frizzled-7, MFRP, LRP 5, LRP 6, Wnt-I, Wnt-8a, Wnt-3a, Wnt-10b, Wnt-4, Wnt-11, Wnt-5a, Wnt-9a and Wnt-7a.

22. The protein according to item 13 wherein the receptor ligand is selected from the group consisting of 4-1BB Ligand/TNFSF9, Lymphotoxin, APRIL/TNFSF13, Lymphotoxin beta/TNFSF3, BAFF/TNFSF13C, OX40 Ligand/TNFSF4, CD27 Ligand/TNFSF7, TL1A/TNFSF15, CD30 Ligand/TNFSF8, TNF-alpha/TNFSFlA,

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CD40 Ligand/TNFSF5, TNF-beta/TNFSFlB, EDA-A2, TRAIL/TNFSF1O, Fas Ligand/TNFSF6, TRANCE/TNFSF11, GITR Ligand/TNFSF18, TWEAK/TNFSF12, LIGHT/TNFSF14, Amphiregulin, NRG1 isoform GGF2, Betacellulin, NRG1 Isoform SMDF, EGF, NRG1-alpha/HRG1-alpha, Epigen, NRGl-beta 1/HRG1-beta 1, Epiregulin, TGF-alpha, HB-EGF, TMEFFI/Tomoregulin-1, Neuregulin-3, TMEFF2, IGF-I, IGF-II, Insulin, Activin A, Activin B, Activin AB, Activin C, BMP-2, BMP-7, BMP-3, BMP-8, BMP-3b/GDF-10, BMP-9, BMP-4, BMP-15, BMP-5, Decapentaplegic, BMP-6, GDF-1, GDF-8, GDF-3, GDF-9, GDF-5, GDF-11, GDF-6, GDF-15, GDF-7, Artemin, Neurturin, GDNF, Persephin, TGF-beta, TGF-beta 2, TGF beta 1, TGF-beta 3, LAP (TGF-beta 1), TGF-beta 5, Latent TGF-beta 1, Latent TGF beta bpl, TGF-beta 1.2, Lefty, Nodal, MIS/AMH, FGF acidic, FGF-12, FGF basic, FGF-13, FGF-3, FGF-16, FGF-4, FGF-17, FGF-5, FGF-19, FGF-6, FGF-20, FGF-8, FGF-21, FGF-9, FGF-23, FGF-10, KGF/FGF-7, FGF-11, Neuropilin-1, PlGF, Neuropilin-2, PlGF-2, PDGF, PDGF-A, VEGF, PDGF-B, VEGF-B, PDGF-C, VEGF C, PDGF-D, VEGF-D and PDGF-AB.

23. The protein according to item 13 wherein the T-cell receptor is selected from the group consisting of 2B4/SLAMF4, IL-2 R alpha, 4-1BB/TNFRSF9, IL-2 R beta, ALCAM, B7-1/CD80, IL-4 R, B7-H3, BLAME/SLAMF8, BTLA, IL-6 R, CCR3, IL-7 R alpha, CCR4, CXCR1/IL-8 RA, CCR5, CCR6, IL-10 R alpha, CCR7, IL-10 R beta, CCR8, IL-12 R beta 1, CCR9, IL-12 R beta 2, CD2, IL-13 R alpha 1, IL-13, CD3, CD4, ILT2/CD85j, ILT3/CD85k, ILT4/CD85d, ILT5/CD85a, Integrin alpha 4/CD49d, CD5, Integrin alpha E/CD103, CD6, Integrin alpha M/CD1Ib, CD8, Integrin alpha X/CD1Ic, Integrin beta 2/CD18, KIR/CD158, CD27/TNFRSF7, KIR2DL1, CD28, KIR2DL3, CD30/TNFRSF8, KIR2DL4/CD158d, CD31/PECAM-1, KIR2DS4, CD40 Ligand/TNFSF5, LAG-3, CD43, LAIRi, CD45, LAIR2, CD83, Leukotriene B4 RI, CD84/SLAMF5, NCAM-L I, CD94, NKG2A, CD97, NKG2C, CD229/SLAMF3, NKG2D, CD2F-IO/SLAMF9, NT-4, CD69, NTB-A/SLAMF6, Common gamma Chain/IL-2 R gamma, Osteopontin, CRACC/SLAMF7, PD-1, CRTAM, PSGL-1, CTLA-4, RANK/TNFRSF1A, CX3CRI, CX3CLI, L-Selectin, CXCR3, SIRP beta 1, CXCR4, SLAM, CXCR6, TCCR/WSX-1, DNAM-1, Thymopoietin, EMMPRIN/CD147, TIM-1, EphB6, TIM-2, Fas/TNFRSF6, TIM-3, Fas Ligand/TNFSF6, TIM-4, Fc gamma RIII/CD16, TIM-6, GITR/TNFRSF18, TNF

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RI/TNFRSFlA, Granulysin, TNF RII/TtNFRSF1B, HVEM/TNFRSF14, TRAIL R1/TNFRSF1OA, ICAM-1/CD54, TRAIL R2/TNFRSF1OB, ICAM-2/CD102, TRAIL R3/TNFRSF1OC, IFN-gamma RI, TRAIL R4/TNFRSF10D, IFN-gamma R2, TSLP, IL-i RI and TSLP R.

24. The protein according to item 13 wherein the NK cell receptor is selected from the group consisting of 2B4/SLAMF4, KIR2DS4, CD155/PVR, KIR3DL1, CD94, LMIRI/CD300A, CD69, LMIR2/CD300c, CRACC/SLAMF7, LMIR3/CD300LF, DNAM-1, LMIR5/CD300LB, Fc epsilon RII, LMIR6/CD300LE, Fc gamma RI/CD64, MICA, Fc gamma RIIB/CD32b, MICB, Fc gamma RIIC/CD32c, MULT-1, Fc gamma RIIA/CD32a, Nectin-2/CD112, Fc gamma RIII/CD16, NKG2A, FcRH1/IRTA5, NKG2C, FcRH2/IRTA4, NKG2D, FcRH4/IRTA1, NKp30, FcRH5/IRTA2, NKp44, Fc Receptor-like 3/CD16-2, NKp46/NCR1, NKp80/KLRF1, NTB-A/SLAMF6, Rae-, Rae-i alpha, Rae-i beta, Rae-i delta, H60, Rae-i epsilon, ILT2/CD85j, Rae-i gamma, ILT3/CD85k, TREM-1, ILT4/CD85d, TREM-2, ILT5/CD85a, TREM-3, KIR/CD158, TREMLI/TLT-1, KIR2DLI, ULBP-1, KIR2DL3, ULBP-2, KIR2DL4/CD158d and ULBP-3.

25. The protein according to item 13 wherein the monocyte/macrophage cell receptor is selected from the group consisting of B7-1/CD80, ILT4/CD85d, B7-H1, ILT5/CD85a, Common beta Chain, Integrin alpha 4/CD49d, BLAME/SLAMF8, Integrin alpha X/CD11c, CCL6/C10, Integrin beta 2/CD18, CD155/PVR, Integrin beta 3/CD61, CD31/PECAM-1, Latexin, CD36/SR-B3, Leukotriene B4 RI, CD40/TNFRSF5, LIMPII/SR-B2, CD43, LMIRI/CD300A, CD45, LMIR2/CD300c, CD68, LMIR3/CD300LF, CD84/SLAMF5, LMIR5/CD300LB, CD97, LMIR6/CD300LE, CD163, LRP-1, CD2F-10/SLAMF9, MARCO, CRACC/SLAMF7, MD-1, ECF-L, MD-2, EMMPRIN/CD147, MGL2, Endoglin/CD105, Osteoactivin/GPNMB, Fc gamma RI/CD64, Osteopontin, Fc gamma RIIB/CD32b, PD L2, Fc gamma RIIC/CD32c, Siglec-3/CD33, Fc gamma RIIA/CD32a, SIGNRI/CD209, Fc gamma RIII/CD16, SLAM, GM-CSF R alpha, TCCR/WSX-1, ICAM-2/CD102, TLR3, IFN-gamma RI, TLR4, IFN-gamma R2, TREM-1, IL-1 RII, TREM-2, ILT2/CD85j, TREM-3, ILT3/CD85k, TREMLI/TLT-1, 2B4/SLAMF4, IL-10 R alpha, ALCAM, IL-10 R beta, Aminopeptidase N/ANPEP, ILT2/CD85j, Common beta Chain,

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198g

ILT3/CD85k, Clq RI/CD93, ILT4/CD85d, CCRI, ILT5/CD85a, CCR2, Integrin alpha 4/CD49d, CCR5, Integrin alpha M/CDIIb, CCR8, Integrin alpha X/CDIIc, CDI55/PVR, Integrin beta 2/CD18, CD14, Integrin beta 3/CD61, CD36/SR-B3, LAIRi, CD43, LAIR2, CD45, Leukotriene B4 RI, CD68, LIMPII/SR-B2, CD84/SLAMF5, LMIRI/CD300A, CD97, LMIR2/CD300c, CD163, LMIR3/CD300LF, Coagulation Factor III/Tissue Factor, LMIR5/CD300LB, CX3CR1, CX3CLI, LMIR6/CD300LE, CXCR4, LRP-i, CXCR6, M-CSF R, DEP-i/CD148, MD-1, DNAM-1, MD-2, EMMPRIN/CD147, MMR, Endoglin/CD105, NCAM-L1, Fc gamma RI/CD64, PSGL-1, Fc gamma RIII/CD16, RP105, G-CSF R, L-Selectin, GM CSF R alpha, Siglec-3/CD33, HVEM/TNFRSF14, SLAM, ICAM-i/CD54, TCCR/WSX-1, ICAM-2/CD102, TREM-1, IL-6 R, TREM-2, CXCRi/IL-8 RA, TREM-3 and TREML1/TLT-1.

26. The protein according to item 13 wherein the Dendritic cell receptor is selected from the group consisting of CD36/SR-B3, LOX-i/SR-El, CD68, MARCO, CD163, SR-AI/MSR, CD5L, SREC-I, CL-Pi/COLECi2, SREC-II, LIMPII/SR-B2, RP105, TLR4, TLRi, TLR5, TLR2, TLR6, TLR3, TLR9,4-iBB Ligand/TNFSF9, IL 12/IL-23 p40, 4-Amino-1,8-naphthalimide, ILT2/CD85j, CCL21/6Ckine, ILT3/CD85k, 8-oxo-dG, ILT4/CD85d, 8D6A, ILT5/CD85a, A2B5, Integrin alpha 4/CD49d, Aag, Integrin beta 2/CD18, AMICA, Langerin, B7-2/CD86, Leukotriene B4 RI, B7-H3, LMIRi/CD300A, BLAME/SLAMF8, LMIR2/CD300c, Clq Ri/CD93, LMIR3/CD300LF, CCR6, LMIR5/CD300LB, CCR7, LMIR6/CD300LE, CD40/TNFRSF5, MAG/Siglec-4a, CD43, MCAM, CD45, MD-1, CD68, MD-2, CD83, MDL-i/CLEC5A, CD84/SLAMF5, MMR, CD97, NCAM-Li, CD2F-O/SLAMF9, Osteoactivin/GPNMB, Chem 23, PD-L2, CLEC-1, RP105, CLEC-2, Siglec-2/CD22, CRACC/SLAMF7, Siglec-3/CD33, DC-SIGN, Siglec-5, DC-SIGNR/CD299, Siglec-6, DCAR, Siglec-7, DCIR/CLEC4A, Siglec-9, DEC-205, Siglec-10, Dectin-i/CLEC7A, Siglec-F, Dectin-2/CLEC6A, SIGNRi/CD209, DEP-i/CD148, SIGNR4, DLEC, SLAM, EMMPRIN/CD147, TCCR/WSX-1, Fc gamma RI/CD64, TLR3, Fc gamma RIIB/CD32b, TREM-1, Fc gamma RIIC/CD32c, TREM-2, Fc gamma RIIA/CD32a, TREM-3, Fc gamma RIII/CD16, TREMLI/TLT-1, ICAM-2/CD102 and Vanilloid RI.

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198h

27. The protein according to item 13 wherein the angiogenesis target is selected from the group consisting of Angiopoietin-1, Angiopoietin-like 2, Angiopoietin-2, Angiopoietin-like 3, Angiopoietin-3, Angiopoietin-like 7/CDT6, Angiopoietin-4, Tie-1, Angiopoietin-like 1, Tie-2, Angiogenin, iNOS, Coagulation Factor III/Tissue Factor, nNOS, CTGF/CCN2, NOV/CCN3, DANCE, OSM, EDG-1, Plfr, EG-VEGF/PK1, Proliferin, Endostatin, ROBO4, Erythropoietin, Thrombospondin 1, Kininostatin, Thrombospondin-2, MFG-E8, Thrombospondin-4, Nitric Oxide, VG5Q, eNOS, EphAl, EphA5, EphA2, EphA6, EphA3, EphA7, EphA4, EphA8, EphB1, EphB4, EphB2, EphB6, EphB3, Ephrin-A1, Ephrin-A4, Ephrin-A2, Ephrin-A5, Ephrin-A3, Ephrin-B1, Ephrin-B3, Ephrin-B2, FGF acidic, FGF-12, FGF basic, FGF 13, FGF-3, FGF-16, FGF-4, FGF-17, FGF-5, FGF-19, FGF-6, FGF-20, FGF-8, FGF 21, FGF-9, FGF-23, FGF-10, KGF/FGF-7, FGF-11, FGF RI, FGF R4, FGF R2, FGF R5, FGF R3, Neuropilin-1, Neuropilin-2, Semaphorin 3A, Semaphorin 6B, Semaphorin 3C, Semaphorin 6C, Semaphorin 3E, Semaphorin 6D, Semaphorin 6A, Semaphorin 7A, MMP, MMP-11, MMP-1, MMP-12, MMP-2, MMP-13, MMP-3, MMP-14, MMP-7, MMP-15, MMP-8, MMP-16/MT3-MMP, MMP-9, MMP-24/MT5-MMP, MMP-10, MMP-25/MT6-MMP, TIMP-1, TIMP-3, TIMP-2, TIMP-4, ACE, IL-13 R alpha 1, IL 13, Ciq R/CD93, Integrin alpha 4/CD49d, VE-Cadherin, Integrin beta 2/CD18, CD31/PECAM-1, KLF4, CD36/SR-B3, LYVE-1, CD151, MCAM, CL-P1/COLEC12, Nectin-2/CD112, Coagulation Factor III/Tissue Factor, E-Selectin, D6, P-Selectin, DC SIGNR/CD299, SLAM, EMMPRIN/CD147, Tie-2, Endoglin/CD105, TNF RI/TNFRSFlA, EPCR, TNF RII/TNFRSFlB, Erythropoietin R, TRAIL R1/TNFRSF1OA, ESAM, TRAIL R2/TNFRSF1OB, FABP5, VCAM-1, ICAM-1/CD54, VEGF R2/Flk-1, ICAM-2/CD102, VEGF R3/Flt-4, IL-i RI and VG5Q.

28. The protein according to item 1 wherein at least one of the first binding domain and the second binding domain specifically binds a target selected from the group consisting of Prostate-specific Membrane Antigen (Folate Hydrolase 1), Epidermal Growth Factor Receptor, Receptor for Advanced Glycation End products, IL-17 A, IL-17 F, P19, Dickkopf-1, NOTCH1, NG2, IgHE, IL-22R, IL-21, an Amyloid P oligomer, an Amyloid P Precursor Protein, NOGO Receptor (RTN4R), Low Density LipoproteinReceptor-Related Protein 5, IL-4, Myostatin, Very Late Antigen 4 and IGF IR.

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198i

29. The protein according to item 12 wherein the cancer cell is a transformed hematopoietic cell.

30. The protein according to item 29 wherein at least one of the first binding domain and the second binding domain recognizes a target selected from the group consisting of a B-cell target, a T-cell target, a dendritic cell target, or an NK-cell target.

31. The protein according to item 29 wherein at least one of the first binding domain and the second binding domain recognizes a target selected from the group consisting of CD5, CD10, CD11c, CD15, CD19, CD20, CD21, CD22, CD23, CD25, CD27, CD30, CD38, CD45, CD70, CD80, CD86, CD103, IRTA1, IRTA2, IRTA3, IRTA4, IRTA5, B-B2, B-B8, and B-cell antigen receptor.

32, The protein according to item 1 comprising a sequence selected from the group consisting of SEQ ID NOS:2, 4, 6, 103, 105, 107, 109, 332, 333, 334, and 345.

33. The protein according to item 1 comprising a sequence selected from the group consisting of SEQ ID NOS:355, 356, 357, 358, 359, 360, 361, 362, 363, 364 and 365.

34. The protein according to item 1 wherein the constant sub-region recognizes an effector cell Fc receptor.

35. The protein according to item 1 wherein the constant sub-region recognizes an effector cell surface protein selected from the group consisting of CD16, CD32a, CD32b, CD64, CD89, Fc E RI, FcRn, and pIgR.

36. The protein according to item 1 wherein the constant sub-region comprises aCH2domain and aCH3domain.

37. The protein according to item 36, wherein theCH3domain is truncated and comprises a C-terminal sequence selected from the group consisting of SEQ ID NOS: 366, 367, 368, 369, 370 and 371.

38. The protein according to item 1, wherein the scorpion linker is derived from an immunoglobulin hinge.

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198j

39. The protein according to item 38, wherein the scorpion linker has a reduced, but non-zero, number of cysteines relative to the hinge from which the scorpion linker is derived, and wherein one cysteine corresponds to the N-terminal hinge cysteine of the immunoglobulin hinge.

40. The protein according to item 39 wherein the scorpion linker is derived from an IgG Ihinge region and wherein the linker comprises 1 or 2 cysteines, further wherein the linker retains the cysteine corresponding to an N-terminal hinge cysteine of the IgG1 hinge region.

41. The protein according to item 1, wherein the scorpion linker is derived from the stalk region of a C-lectin.

42. The protein according to item 1, wherein the scorpion linker comprises a sequence selected from the group consisting of SEQ ID NOS:373, 374, 375, 376 and 377.

43. The protein according to item 1, wherein the scorpion linker comprises a sequence selected from the group consisting of SEQ ID NOS:351, 352, 353 and 354.

44. The protein according to item 1 further comprising a linker of at least about 5 amino acids attached to the constant sub-region and attached to the first binding domain, thereby localizing the linker between the constant sub-region and the first binding domain.

45. The protein according to item 1 in which the binding affinity of at least one of the first binding domain and the second binding domain is less than 10-9 M.

46. The protein according to item 1 in which the binding affinity of at least one of the first binding domain and the second binding domain is at least 106 M.

47. The protein according to item 1, wherein the effector function is selected from the group consisting of antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC).

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198k

48. The protein according to item 1, wherein the in vivo half-life of the protein is at least 28 hours in a human.

49. A pharmaceutical composition comprising the protein according to item 1 and a pharmaceutically acceptable adjuvant, carrier or excipient.

50. A nucleic acid encoding a protein according to item 1.

51. A vector comprising a nucleic acid according to item 50.

52. A host cell comprising a vector according to item 51.

53. A method of producing the protein according to item1 comprising:

a. introducing a nucleic acid encoding the protein according to item 1 into a host cell; and

b. incubating the host cell under conditions suitable for expression of the protein, thereby expressing the protein at a level of at least 1 mg/ml.

54. The method according to item 53 further comprising isolating the protein.

55. The method according to item 53 wherein the host cell is selected from the group consisting of a VERO cell, a HeLa cell, a CHO cell, a COS cell, a W138 cell, a BHK cell, a HepG2 cell, a 3T3 cell, a RIN cell, an MDCK cell, an A549 cell, a PC12 cell, a K562 cell, a HEK293 cell, an N cell, a Spodopterafrugiperdacell, a Saccharomyces cerevisiaecell, a Pichiapastoris cell, a fungal cell and a bacterial cell.

56. A method of producing a nucleic acid encoding the protein according to item 1 comprising:

a. covalently linking the 3' end of a polynucleotide encoding a first binding domain derived from an immunoglobulin variable region to the 5' end of a polynucleotide encoding a constant sub-region;

C:\NRPortbl\GHMatters\KIRSTENA\17432161_1.docx 19/02/21 b. covalently linking the 5' end of a polynucleotide encoding a scorpion linker to the 3' end of the polynucleotide encoding the constant sub-region; and c. covalently linking the 5' end of a polynucleotide encoding a second binding domain derived from an immunoglobulin variable region to the 3' end of the polynucleotide encoding the scorpion linker, thereby generating a nucleic acid encoding a multivalent binding protein with effector function.

57. The method according to item 56 wherein the polynucleotide encoding a first binding domain comprises a sequence selected from the group consisting of SEQ ID NO:1 (anti-CD20 variable region), SEQ ID NO:3 (anti-CD28 variable region, orientedVL-VH) and SEQ ID NO:5 (anti-CD28 variable region, orientedVH-VL).

58. The method according to item 56, further comprising a linker polynucleotide inserted between the polynucleotide encoding a first binding domain and the polynucleotide encoding a constant sub-region, the linker polynucleotide encoding a peptide linker of at least 5 amino acids.

59. The method according to item 56, further comprising a linker polynucleotide inserted between the polynucleotide encoding a constant sub-region and the polynucleotide encoding a second binding domain, the linker polynucleotide encoding a peptide linker of at least 5 amino acids.

60. A method of inducing damage to a target cell comprising contacting a target cell with a therapeutically effective amount of a protein according to item 1.

61. The method according to item 60 wherein the target cell is contacted in vivo by administration of the protein to an organism in need.

62. A method of treating a cell proliferation disorder comprising administering a therapeutically effective amount of a protein according to item 1 to an organism in need.

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198m

63. The method according to item 62 wherein the disorder is selected from the group consisting of a cancer, an autoimmune disorder, Rous Sarcoma Virus infection and inflammation.

64. The method according to item 62 wherein the protein is administered by in vivo expression of a nucleic acid encoding the protein according to item 1.

65. The method according to item 62 wherein the protein is administered by a route selected from the group consisting of intravenous injection, intraarterial injection, intramuscular injection, subcutaneous injection, intraperitoneal injection and direct tissue injection.

66. A method of ameliorating a symptom associated with a cell proliferation disorder comprising administering a therapeutically effective amount of a protein according to item 1 to an organism in need.

67. The method according to item 66, wherein the symptom is selected from the group consisting of pain, heat, swelling and joint stiffness.

68. A method of treating an infection associated with an infectious agent comprising administering a therapeutically effective amount of the protein according to item 1 to a patient in need, wherein the protein comprises a binding domain that specifically binds a target molecule of the infectious agent.

69. A method of ameliorating a symptom of an infection associated with an infectious agent comprising administering an effective amount of the protein according to item 1 to a patient in need, wherein the protein comprises a binding domain that specifically binds a target molecule of the infectious agent.

70. A method of reducing the risk of infection attributable to an infectious agent comprising administering a prophylactically effective amount of the protein according to item 1 to a patient at risk of developing the infection, wherein the protein comprises a binding domain that specifically binds a target molecule of the infectious agent.

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198n

71. A kit comprising the protein according to item 1 and a set of instructions for administering the protein to treat a cell proliferation disorder or to ameliorate a symptom of the cell proliferation disorder.

72. The multivalent single-chain binding protein according to item 1 wherein at least one of the first binding domain and the second binding domain specifically binds an antigen selected from the group consisting of CD19, CD20, CD21, CD22, CD23, CD30, CD37, CD40, CD70, CD72, CD79a, CD79b, CD80, CD81, CD86, and a major histocompatibility complex class II peptide.

73. The protein according to item 72 wherein one of the first binding domain and the second binding domain specifically binds CD20.

74. The protein according to item 73 wherein the other binding domain specifically binds an antigen selected from the group consisting of CD19, CD20, CD21, CD22, CD23, CD30, CD37, CD40, CD70, CD72, CD79a, CD79b, CD80, CD81, CD86, and a major histocompatibility complex class II peptide.

75. The protein according to item 72 wherein one of the first binding domain and the second binding domain specifically binds CD79b.

76. The protein according to item 75 wherein the other binding domain specifically binds an antigen selected from the group consisting of CD19, CD20, CD21, CD22, CD23, CD30, CD37, CD40, CD70, CD72, CD79a, CD79b, CD80, CD81, CD86, and a major histocompatibility complex class II peptide.

77. The protein according to item 72 wherein one of the first binding domain and the second binding domain specifically binds a major histocompatibility complex class II peptide.

78. The protein according to item 77 wherein the other binding domain specifically binds an antigen selected from the group consisting of CD19, CD20, CD21, CD22, CD23, CD30, CD37, CD40, CD70, CD72, CD79a, CD79b, CD80, CD81, CD86, and a major histocompatibility complex class II peptide.

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198o

79. The protein according to item 72 wherein one of the first binding domain and the second binding domain specifically binds CD22.

80. The protein according to item 79 wherein the other binding domain specifically binds an antigen selected from the group consisting of CD19, CD20, CD21, CD22, CD23, CD30, CD37, CD40, CD70, CD72, CD79a, CD79b, CD80, CD81, CD86 and a major histocompatibility complex class II peptide.

81. The protein according to item 72 wherein the protein has a synergistic effect on a target cell behavior relative to the sum of the effects of each of the binding domains, and wherein the protein comprises a binding domain pair specifically recognizing a pair of antigens selected from the group consisting of CD20-CD19, CD20-CD21, CD20-CD22, CD20-CD40, CD20-CD79a, CD20-CD79b and CD20 CD81.

82. The protein according to item 72 wherein the protein has an additive effect on a target cell behavior relative to the sum of the effects of each of the binding domains, and wherein the protein comprises a binding domain pair specifically recognizing a pair of antigens selected from the group consisting of CD20-CD23, CD20-CD30, CD20-CD37, CD20-CD70, CD20-CD80, CD20-CD86, CD79b-CD37, CD79b-CD81, major histocompatibility complex class II peptide-CD30, and major histocompatibility complex class II peptide-CD72.

83. The protein according to item 72 wherein the protein has an inhibitory effect on a target cell behavior relative to the sum of the effects of each of the binding domains, and wherein the protein comprises a binding domain pair specifically recognizing a pair of antigens selected from the group consisting of CD20-major histocompatibility complex class II peptide, CD79b-CD19, CD79b-CD20, CD79b CD21, CD79b-CD22, CD79b-CD23, CD79b-CD30, CD79b-CD40, CD79b-CD70, CD79b-CD72, CD79b-CD79a, CD79b-CD80, CD79b-CD86, CD79b-major histocompatibility complex class II peptide, major histocompatibility complex class II peptide-CD19, major histocompatibility complex class II peptide-CD20, major histocompatibility complex class II peptide-CD21, major histocompatibility complex class II peptide-CD22, major histocompatibility complex class II peptide-CD23, major

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19 8p

histocompatibility complex class II peptide-CD37, major histocompatibility complex class II peptide-CD40, major histocompatibility complex class II peptide-CD70, major histocompatibility complex class II peptide-CD79a, major histocompatibility complex class II peptide-CD79b, major histocompatibility complex class II peptide-CD80, major histocompatibility complex class II peptide-CD81, major histocompatibility complex class II peptide-CD86, CD22-CD19, CD22-CD40, CD22-CD79b, CD22-CD86 and CD22-major histocompatibility complex class II peptide.

84. A method of identifying at least one of the binding domains of the multivalent binding molecule according to item 1 comprising:

(a) contacting an anti-isotypic antibody with an antibody specifically recognizing a first antigen and an antibody specifically recognizing a second antigen;

(b) further contacting a target comprising at least one of the antigens with the composition of step (a); and

(c) measuring an activity of the target, wherein the activity is used to identify at least one of the binding domains of the multivalent binding molecule.

85. The method according to item 84 wherein the target is a diseased cell.

86. The method according to item 60 wherein the multivalent single-chain binding protein induces a synergistic amount of damage to the target cell compared to the sum of the damage induced by a first antibody comprising the first binding domain but not the second binding domain and a second antibody comprising the second binding domain but not the first binding domain.

87. The method according to item 86 wherein the multivalent single-chain binding protein comprises a binding domain pair specifically recognizing a pair of antigens selected from the group consisting of CD19/CD20, CD20/CD21, CD20/CD22, CD20/CD40, CD20/CD79a, CD20/CD79b, CD20/CD81, CD21/CD79b, CD37/CD79b, CD79b/CD81, CD19/CL II, CD20/CL II, CD30/CL II, CD37/CL II, CD72/CL II, and CD79b/CL II.

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198q

88. The method according to item 60 wherein the multivalent single-chain binding protein induces an inhibited amount of damage to the target cell compared to the sum of the damage induced by a first antibody comprising thefirst binding domain but not the second binding domain and a second antibody comprising the second binding domain but not the first binding domain.

89. The method according to item 88 wherein the multivalent single-chain binding protein comprises a binding domain pair specifically recognizing a pair of antigens selected from the group consisting of CD20/CL II, CD21/CD79b, CD22/CD79b, CD40/CD79b, CD70/CD79b, CD72/CD79b, CD79a/CD79b, CD79b/CD80, CD79b/CD86, CD21/CL II, CD22/CL II, CD23/CL II, CD40/CL II, CD70/CL II, CD80/CL II, CD86/CL II, CD19/CD22, CD20/CD22, CD21/CD22, CD22/CD23, CD22/CD30, CD22/CD37, CD22/CD40, CD22/CD70, CD22/CD72, CD22/79a, CD22/79b, CD22/CD80, CD22/CD86 and CD22/CL II.

90. The method according to item 60 further comprising a plurality of multivalent single-chain binding proteins.

91. The method according to item 90 wherein a binding domain of a first multivalent single-chain binding protein and a binding domain of a second multivalent single-chain binding protein induce a synergistic amount of damage to the target cell.

92. The method according to item 90 wherein a binding domain of a first multivalent single-chain binding protein and a binding domain of a second multivalent single-chain binding protein induce an inhibited amount of damage to the target cell.

93. A composition comprising a plurality of multivalent single-chain binding proteins according to item 1.

94. The composition according to item 93 wherein a binding domain of a first multivalent single-chain binding protein and a binding domain of a second multivalent single-chain binding protein are capable of inducing a synergistic amount of damage to a target cell.

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198r

95. The composition according to item 93 wherein a binding domain of a first multivalent single-chain binding protein and a binding domain of a second multivalent single-chain binding protein are capable of inducing an additive amount of damage to a target cell.

96. The composition according to item 93 wherein a binding domain of a first multivalent single-chain binding protein and a binding domain of a second multivalent single-chain binding protein are capable of inducing an inhibited amount of damage to a target cell.

97. A pharmaceutical composition comprising the composition according to item 93 and a pharmaceutically acceptable carrier, diluent or excipient.

98. A kit comprising the composition according to item 93 and a set of instructions for administering the composition to damage a target cell.

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SEQUENCE LISTING

<110> Thompson et al.

5 <120> Single-Chain Multivalent Binding Proteins with Effector 2021201261

Function

<130> 30906/41393C 10 <160> 379

<170> PatentIn version 3.3

15 <210> 1 <211> 795 <212> DNA <213> Artificial sequence

20 <220> <223> Synthetic polynucleotide

<220> <223> anti-CD20 (2H7) LH (DNA) 25 <400> 1 atggattttc aagtgcagat tttcagcttc ctgctaatca gtgcttcagt cataatgtcc 60

30 agaggacaaa ttgttctctc ccagtctcca gcaatcctgt ctgcatctcc aggggagaag 120

gtcacaatga cttgcagggc cagctcaagt gtaagttaca tgcactggta ccagcagaag 180 35 ccaggatcct cccccaaacc ctggatttat gccccatcca acctggcttc tggagtccct 240

gctcgcttca gtggcagtgg gtctgggacc tcttactctc tcacaatcag cagagtggag 300

5 gctgaagatg ctgccactta ttactgccag cagtggagtt ttaacccacc cacgttcggt 360 2021201261

gctgggacca agctggagct gaaagatggc ggtggctcgg gcggtggtgg atctggagga 420 10 ggtgggagct ctcaggctta tctacagcag tctggggctg agtcggtgag gcctggggcc 480

tcagtgaaga tgtcctgcaa ggcttctggc tacacattta ccagttacaa 15 tatgcactgg 540

gtaaagcaga cacctagaca gggcctggaa tggattggag ctatttatcc aggaaatggt 600

20 gatacttcct acaatcagaa gttcaagggc aaggccacac tgactgtaga caaatcctcc 660

agcacagcct acatgcagct cagcagcctg acatctgaag actctgcggt ctatttctgt 720 25 gcaagagtgg tgtactatag taactcttac tggtacttcg atgtctgggg cacagggacc 780

acggtcaccg tctct 30 795

<210> 2 <211> 265 35 <212> PRT <213> Artificial sequence

<220> <223> Synthetic polypeptide

<220> 5 <223> anti-CD20 (2H7) LH (AA) 2021201261

<220> <221> misc_feature <222> (1)..(22) 10 <223> Leader

<220> <221> misc_feature <222> (23)..(128) 15 <223> VL

<220> <221> misc_feature <222> (129)..(144) 20 <223> Linker

<220> <221> misc_feature <222> (145)..(265) 25 <223> VH

<400> 2

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser 30 Ala Ser 1 5 10 15

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 35 20 25 30

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg

Ala Ser 35 40 45

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly 5 Ser Ser 50 55 60 2021201261

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 10 65 70 75 80

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 15 85 90 95

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105 110 20 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 115 120 125

25 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro 30 Gly Ala 145 150 155 160

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 35 Ser Tyr 165 170

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 5 Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln 2021201261

Lys Phe 195 200 205

10 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 15 Phe Cys 225 230 235 240

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp 20 Val Trp 245 250 255

Gly Thr Gly Thr Thr Val Thr Val Ser 25 260 265

<210> 3 <211> 813 <212> DNA 30 <213> Artificial sequence

<220> <223> Synthetic polynucleotide

35 <220> <223> anti-CD28 (2e12) LH (DNA)

<400> 3 atggattttc aagtgcagat tttcagcttc ctgctaatca gtgcttcagt cataatgtcc 60

5 agaggagtcg acattgtgct cacccaatct ccagcttctt tggctgtgtc tctaggtcag 120 2021201261

agagccacca tctcctgcag agccagtgaa agtgttgaat attatgtcac aagtttaatg 180 10 cagtggtacc aacagaaacc aggacagcca cccaaactcc tcatctctgc tgctagcaac 240

gtagaatctg gggtccctgc caggtttagt ggcagtgggt ctgggacaga 15 ctttagcctc 300

aacatccatc ctgtggagga ggatgatatt gcaatgtatt tctgtcagca aagtaggaag 360

20 gttccatgga cgttcggtgg aggcaccaag ctggaaatca aacggggtgg cggtggatcc 420

ggcggaggtg ggtcgggtgg cggcggatct caggtgcagc tgaaggagtc aggacctggc 480 25 ctggtggcgc cctcacagag cctgtccatc acatgcaccg tctcagggtt ctcattaacc 540

ggctatggtg taaactgggt tcgccagcct ccaggaaagg gtctggagtg 30 gctgggaatg 600

atatggggtg atggaagcac agactataat tcagctctca aatccagact atcgatcacc 660

35 aaggacaact ccaagagcca agttttctta aaaatgaaca gtctgcaaac tgatgacaca 720

gccagatact actgtgcccg agatggttat agtaactttc attactatgt tatggactac 780

tggggtcaag gaacctcagt caccgtctcc tct 5 813 2021201261

<210> 4 <211> 271 <212> PRT 10 <213> Artificial sequence

<220> <223> Synthetic polypeptide

15 <220> <223> anti-CD28 (2e12) LH (AA)

<220> <221> misc_feature 20 <222> (1)..(23) <223> Leader

<220> <221> misc_feature 25 <222> (24)..(135) <223> VL

<220> <221> misc_feature 30 <222> (136)..(150) <223> Linker

<220> <221> misc_feature 35 <222> (151)..(271) <223> VH

<400> 4

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 5 1 5 10 15 2021201261

Val Ile Met Ser Arg Gly Val Asp Ile Val Leu Thr Gln Ser Pro Ala 10 20 25 30

Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala 15 35 40 45

Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln 20 50 55 60

Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 65 70 75 25 80

Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 30 85 90 95

Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met 35 100 105 110

Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly 115 120 125

5 Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly 2021201261

Gly Gly 130 135 140

10 Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly 145 150 155 160 15

Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly 165 170 20 175

Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly 25 180 185 190

Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp 30 195 200 205

Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser 35 210 215 220

Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 225 230 235 240 5 2021201261

Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr 245 250 10 255

Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 15 260 265 270

<210> 5 <211> 828 20 <212> DNA <213> Artificial sequence

<220> <223> Synthetic polynucleotide 25 <220> <223> anti-CD28 (2e12) HL (DNA)

<400> 5 30 atggattttc aagtgcagat tttcagcttc ctgctaatca gtgcttcagt cataatgtcc 60

agaggagtcc aggtgcagct gaaggagtca ggacctggcc tggtggcgcc ctcacagagc 120 35 ctgtccatca catgcaccgt ctcagggttc tcattaaccg gctatggtgt aaactgggtt 180

cgccagcctc caggaaaggg tctggagtgg ctgggaatga tatggggtga tggaagcaca 240

5 gactataatt cagctctcaa atccagacta tcgatcacca aggacaactc caagagccaa 300 2021201261

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccagatacta ctgtgcccga 360 10 gatggttata gtaactttca ttactatgtt atggactact ggggtcaagg aacctcagtc 420

accgtctcct ctgggggtgg aggctctggt ggcggtggat ccggcggagg 15 tgggtcgggt 480

ggcggcggat ctgacattgt gctcacccaa tctccagctt ctttggctgt gtctctaggt 540

20 cagagagcca ccatctcctg cagagccagt gaaagtgttg aatattatgt cacaagttta 600

atgcagtggt accaacagaa accaggacag ccacccaaac tcctcatctc tgctgctagc 660 25 aacgtagaat ctggggtccc tgccaggttt agtggcagtg ggtctgggac agactttagc 720

ctcaacatcc atcctgtgga ggaggatgat attgcaatgt atttctgtca 30 gcaaagtagg 780

aaggttccat ggacgttcgg tggaggcacc aagctggaaa tcaaacgt 828

35 <210> 6 <211> 276

<212> PRT <213> Artificial sequence

<220> 5 <223> Synthetic polypeptide 2021201261

<220> <223> anti-CD28 (2e12) HL (AA)

10 <220> <221> misc_feature <222> (1)..(23) <223> Leader

15 <220> <221> misc_feature <222> (24)..(144) <223> VH

20 <220> <221> misc_feature <222> (145)..(164) <223> Linker

25 <220> <221> misc_feature <222> (165)..(276) <223> VL

30 <400> 6

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 35

Val Ile Met Ser Arg Gly Val Gln Val Gln Leu Lys Glu Ser

Gly Pro 20 25 30

5 Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser 2021201261

35 40 45

10 Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro 50 55 60

15 Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 65 70 75 80

20 Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 85 90 95

25 Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 100 105 110

30 Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr 115 120 125

35 Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 130 135 140

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 5 145 150 155 160 2021201261

Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser 10 Leu Ala 165 170 175

15 Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser 180 185 190

20 Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 195 200 205

25 Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 210 215 220

30 Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 225 230 235 240

35 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys

245 250 255

5 Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 2021201261

260 265 270

10 Glu Ile Lys Arg 275

<210> 7 15 <211> 30 <212> DNA <213> Artificial sequence

<220> 20 <223> Synthetic primer

<220> <223> Heavy Chain GSP1

25 <400> 7 aggtgctgga ggggacagtc actgagctgc 30

30 <210> 8 <211> 28 <212> DNA <213> Artificial sequence

35 <220> <223> Synthetic primer

<220> <223> Nested Heavy Chain

<400> 8 5 gtcacwgtca ctgrctcagg gaartagc 28 2021201261

<210> 9 10 <211> 33 <212> DNA <213> Artificial sequence

<220> 15 <223> Synthetic primer

<220> <223> Light Chain GSP1

20 <400> 9 gggtgctgct catgctgtag gtgctgtctt tgc 33

25 <210> 10 <211> 32 <212> DNA <213> Artificial sequence

30 <220> <223> Synthetic primer

<220> <223> Nested Light Chain 35 <400> 10 caagaagcac acgactgagg cacctccaga tg

<210> 11 5 <211> 36 <212> DNA 2021201261

<213> Artificial sequence

<220> 10 <223> Synthetic primer

<220> <223> Race Abridged Anchor Primer

15 <220> <221> misc_feature <222> (24)..(25) <223> n is a, c, g, or t

20 <220> <221> misc_feature <222> (29)..(30) <223> n is a, c, g, or t

25 <220> <221> misc_feature <222> (34)..(35) <223> n is a, c, g, or t

30 <400> 11 ggccacgcgt cgactagtac gggnngggnn gggnng 36

35 <210> 12 <211> 20 <212> DNA

<213> Artificial sequence

<220> <223> Synthetic primer 5 <220> 2021201261

<223> T7 primer

<400> 12 10 gtaatacgac tcactatagg 20

<210> 13 15 <211> 18 <212> DNA <213> Artificial sequence

<220> 20 <223> Synthetic primer

<220> <223> M13 reverse primer

25 <400> 13 caggaaacag ctatgacc 18

30 <210> 14 <211> 15 <212> PRT <213> Homo sapiens

35 <220> <223> human IgG1 hinge domain

<400> 14

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 5 1 5 10 15 2021201261

<210> 15 <211> 46 10 <212> DNA <213> Homo sapiens

<220> <223> hVK3L-F3H3 15 <400> 15 gcgataaagc ttgccgccat ggaagcacca gcgcagcttc tcttcc 46

20 <210> 16 <211> 50 <212> DNA <213> Homo sapiens 25 <220> <223> hVK3LF2

30 <400> 16 accagcgcag cttctcttcc tcctgctact ctggctccca gataccaccg 50

35 <210> 17 <211> 45 <212> DNA

<213> Homo sapiens

<220> <223> hVK3LF12HVL 5 <400> 17 2021201261

ggctcccaga taccaccggt caaattgttc tctcccagtc tccag 45

10 <210> 18 <211> 35 <212> DNA <213> Artificial sequence 15 <220> <223> Synthetic primer

<220> 20 <223> 2H7VHNheF

<400> 18 gcgatagcta gccaggctta tctacagcag tctgg 35 25

<210> 19 <211> 43 <212> DNA 30 <213> Artificial sequence

<220> <223> Synthetic primer

35 <220> <223> G4SNheR

<400> 19 gcgatagcta gccccacctc ctccagatcc accaccgccc gag 43

5 <210> 20 2021201261

<211> 35 <212> DNA <213> Artificial sequence 10 <220> <223> Synthetic primer

<220> 15 <223> 015VHXhoR

<400> 20 gcgtactcga ggagacggtg accgtggtcc ctgtg 35 20

<210> 21 <211> 37 <212> DNA 25 <213> Artificial sequence

<220> <223> Synthetic primer

30 <220> <223> G1HC-Xho

<400> 21 gcagtctcga gcgagcccaa atcttgtgac aaaactc 35 37

<210> 22 <211> 37 <212> DNA <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic primer

<220> 10 <223> G1H-S-Xho

<400> 22 gcagtctcga gcgagcccaa atcttctgac aaaactc 37 15

<210> 23 <211> 36 <212> DNA 20 <213> Artificial sequence

<220> <223> Synthetic primer

25 <220> <223> CH3REcoR1

<400> 23 gcgtgagaat tcttacccgg agacagggag aggctc 30 36

<210> 24 <211> 32 35 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer

<220> 5 <223> G1 XBZ-R 2021201261

<400> 24 gcgacgtcta gagtcattta cccggagaca gg 32 10

<210> 25 <211> 54 <212> DNA 15 <213> Artificial sequence

<220> <223> Synthetic primer

20 <220> <223> G4SLinkR1-S

<400> 25 aattatggtg gcggtggctc gggcggtggt ggatctggag gaggtgggag 25 tggg 54

<210> 26 <211> 54 30 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 35 <220> <223> G4SLinkR1-AS

<400> 26 aattcccact cccacctcct ccagatccac caccgcccga gccaccgcca ccat 54 5 2021201261

<210> 27 <211> 36 <212> DNA 10 <213> Artificial sequence

<220> <223> Synthetic primer

15 <220> <223> 2E12VLXbaR

<400> 27 gcgtgtctag attaacgttt gatttccagc ttggtg 20 36

<210> 28 <211> 35 25 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 30 <220> <223> 2E12VLR1F

<400> 28 35 gcgatgaatt ctgacattgt gctcacccaa tctcc

<210> 29 <211> 34 <212> DNA 5 <213> Artificial sequence 2021201261

<220> <223> Synthetic primer

10 <220> <223> 2E12VHR1F

<400> 29 gcgatgaatt ctcaggtgca gctgaaggag tcag 15 34

<210> 30 <211> 37 20 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 25 <220> <223> 2E12VHXbaR

<400> 30 30 gcgagtctag attaagagga gacggtgact gaggttc 37

<210> 31 35 <211> 27 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer

5 <220> <223> 2e12VHdXbaF1 2021201261

<400> 31 gggtctggag tggctgggaa tgatatg 10 27

<210> 32 <211> 28 15 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 20 <220> <223> 2e12VHdXbaR1

<400> 32 25 attcccagcc actccagacc ctttcctg 28

<210> 33 30 <211> 23 <212> DNA <213> Artificial sequence

<220> 35 <223> Synthetic primer

<220>

<223> IgBsrG1F

<400> 33 gagaaccaca ggtgtacacc ctg 5 23 2021201261

<210> 34 <211> 25 10 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 15 <220> <223> IgBsrG1R

<400> 34 20 gcagggtgta cacctgtggt tctcg 25

<210> 35 25 <211> 17 <212> DNA <213> Artificial sequence

<220> 30 <223> Synthetic primer

<220> <223> M13R

35 <400> 35 caggaaacag ctatgac

<210> 36 <211> 18 5 <212> DNA <213> Artificial sequence 2021201261

<220> <223> Synthetic primer 10 <220> <223> M13F

<400> 36 15 gtaaaacgac ggccagtg 18

<210> 37 20 <211> 20 <212> DNA <213> Artificial sequence

<220> 25 <223> Synthetic primer

<220> <223> T7

30 <400> 37 gtaatacgac tcactatagg 20

35 <210> 38 <211> 17 <212> DNA

<213> Artificial sequence

<220> <223> Synthetic primer 5 <220> 2021201261

<223> pD18F-17

<400> 38 10 aactagagaa cccactg 17

<210> 39 15 <211> 20 <212> DNA <213> Artificial sequence

<220> 20 <223> Synthetic primer

<220> <223> pD18F-20

25 <400> 39 gctaactaga gaacccactg 20

30 <210> 40 <211> 18 <212> DNA <213> Artificial sequence

35 <220> <223> Synthetic primer

<220> <223> pD18F-1

<400> 40 5 atacgactca ctataggg 18 2021201261

<210> 41 10 <211> 19 <212> DNA <213> Artificial sequence

<220> 15 <223> Synthetic primer

<220> <223> pD18R-s

20 <400> 41 gctctagcat ttaggtgac 19

25 <210> 42 <211> 18 <212> DNA <213> Artificial sequence

30 <220> <223> Synthetic primer

<220> <223> CH3seqF1 35 <400> 42 catgaggctc tgcacaac

<210> 43 5 <211> 18 <212> DNA 2021201261

<213> Artificial sequence

<220> 10 <223> Synthetic primer

<220> <223> CH3seqF2

15 <400> 43 cctctacagc aagctcac 18

20 <210> 44 <211> 19 <212> DNA <213> Artificial sequence

25 <220> <223> Synthetic primer

<220> <223> CH3seqR1 30 <400> 44 ggttcttggt cagctcatc 19

35 <210> 45 <211> 18

<212> DNA <213> Artificial sequence

<220> 5 <223> Synthetic primer 2021201261

<220> <223> CH3seqR2

10 <400> 45 gtgagcttgc tgtagagg 18

15 <210> 46 <211> 51 <212> DNA <213> Artificial sequence

20 <220> <223> Synthetic primer

<220> <223> L1-11R 25 <400> 46 gcgatagaat tcccagatcc accaccgccc gagccaccgc caccataatt c 51

30 <210> 47 <211> 36 <212> DNA <213> Artificial sequence 35 <220> <223> Synthetic primer

<220> <223> L1-6R

5 <400> 47 gcgatagaat tcccagagcc accgccacca taattc 2021201261

36

10 <210> 48 <211> 45 <212> DNA <213> Artificial sequence

15 <220> <223> Synthetic primer

<220> <223> L3R 20 <400> 48 gcgtatgaat tccccgagcc accgccaccc ttacccggag acagg 45

25 <210> 49 <211> 49 <212> DNA <213> Artificial sequence 30 <220> <223> Synthetic primer

<220> 35 <223> L4R

<400> 49

gcgtatgaat tcccagatcc accaccgccc gagccaccgc cacccttac 49

5 <210> 50 <211> 44 2021201261

<212> DNA <213> Artificial sequence

10 <220> <223> Synthetic primer

<220> <223> L5R 15 <400> 50 gcgtatgaat tcccgctgcc tcctccccca gatccaccac cgcc 44

20 <210> 51 <211> 23 <212> DNA <213> Artificial sequence 25 <220> <223> Synthetic primer

<220> 30 <223> IgBsrG1F

<400> 51 gagaaccaca ggtgtacacc ctg 23 35

<210> 52

<211> 50 <212> DNA <213> Artificial sequence

5 <220> <223> Synthetic primer 2021201261

<220> <223> L-CPPCPR 10 <400> 52 gcgatagaat tcggacaagg tggacacccc ttacccggag acagggagag 50

15 <210> 53 <211> 41 <212> DNA <213> Artificial sequence 20 <220> <223> G281LH-NheR

<220> 25 <223> Synthetic primer

<400> 53 actgctgcag ctggaccgcg ctagctccgc cgccacccga c 41 30

<210> 54 <211> 40 <212> DNA 35 <213> Artificial sequence

<220>

<223> Synthetic primer

<220> <223> G281LH-NheF 5 <400> 54 2021201261

ggcggagcta gcgcggtcca gctgcagcag tctggacctg 40

10 <210> 55 <211> 37 <212> DNA <213> Artificial sequence 15 <220> <223> Synthetic primer

<220> 20 <223> G281-LH-LPinF

<400> 55 gcgatcaccg gtgacatcca gatgactcag tctccag 37 25

<210> 56 <211> 37 <212> DNA 30 <213> Artificial sequence

<220> <223> Synthetic primer

35 <220> <223> G281-LH-HXhoR

<400> 56 gcgatactcg aggagacggt gactgaggtt ccttgac 37

5 <210> 57 2021201261

<211> 38 <212> DNA <213> Artificial sequence 10 <220> <223> Synthetic primer

<220> 15 <223> G281-LH-LEcoF

<400> 57 gcgatcgaat tcagacatcc agatgactca gtctccag 38 20

<210> 58 <211> 43 <212> DNA 25 <213> Artificial sequence

<220> <223> Synthetic primer

30 <220> <223> G281-LH-HXbaR

<400> 58 gcgattctag attaggaaga gacggtgact gaggttcctt gac 35 43

<210> 59 <211> 37 <212> DNA <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic primer

<220> 10 <223> G281-HL-HF

<400> 59 gcgataaccg gtgcggtcca gctgcagcag tctggac 37 15

<210> 60 <211> 52 <212> DNA 20 <213> Artificial sequence

<220> <223> Synthetic primer

25 <220> <223> G281-HL-HR3

<400> 60 gacccaccac cgcccgagcc accgccacca gaagagacgg tgactgaggt tc 30 52

<210> 61 <211> 49 35 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer

<220> 5 <223> G281-HL-HR2 2021201261

<400> 61 actcccgcct cctcctgatc cgccgccacc cgacccacca ccgcccgag 49 10

<210> 62 <211> 42 <212> DNA 15 <213> Artificial sequence

<220> <223> Synthetic primer

20 <220> <223> G281-HL-HNheR

<400> 62 gagtcatctg gatgtcgcta gcactcccgc ctcctcctga tc 25 42

<210> 63 <211> 45 30 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 35 <220> <223> G281-HL-LNheF

<400> 63 atcaggagga ggcgggagtg ctagcgacat ccagatgact cagtc 45 5 2021201261

<210> 64 <211> 37 <212> DNA 10 <213> Artificial sequence

<220> <223> Synthetic primer

15 <220> <223> G281-HL-LXhoR

<400> 64 gcgatactcg agcctttgat ctccagttcg gtgcctc 20 37

<210> 65 <211> 42 25 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 30 <220> <223> G281-HL-LXhoR

<400> 65 35 gcgatatcta gactcaacct ttgatctcca gttcggtgcc tc

<210> 66 <211> 37 <212> DNA 5 <213> Artificial sequence 2021201261

<220> <223> Synthetic primer

10 <220> <223> G281-HL-EcoF

<400> 66 gcgatagaat tcgcggtcca gctgcagcag tctggac 15 37

<210> 67 <211> 40 20 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 25 <220> <223> 194-LH-LF1

<400> 67 30 gcgtatgaac cggtgacatc cagatgacac agactacatc 40

<210> 68 35 <211> 50 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer

5 <220> <223> 194-LF2 2021201261

<400> 68 atccagatga cacagactac atcctccctg tctgcctctc tgggagacag 10 50

<210> 69 <211> 52 15 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 20 <220> <223> 194-LF3

25 <400> 69 gtctgcctct ctgggagaca gagtcaccat cagttgcagg gcaagtcagg ac 52

30 <210> 70 <211> 50 <212> DNA <213> Artificial sequence

35 <220> <223> Synthetic primer

<220> <223> 194-LF4

<400> 70 5 gttgcagggc aagtcaggac attcgcaatt atttaaactg gtatcagcag 50 2021201261

<210> 71 10 <211> 50 <212> DNA <213> Artificial sequence

<220> 15 <223> Synthetic primer

<220> <223> 194-LF5

20 <400> 71 atttaaactg gtatcagcag aaaccagatg gaactgttaa actcctgatc 50

25 <210> 72 <211> 50 <212> DNA <213> Artificial sequence

30 <220> <223> Synthetic primer

<220> <223> 194-LF6 35 <400> 72 gaactgttaa actcctgatc tactacacat caagattaca ctcaggagtc

<210> 73 5 <211> 52 <212> DNA 2021201261

<213> Artificial sequence

<220> 10 <223> Synthetic primer

<220> <223> 194-LF7

15 <400> 73 caagattaca ctcaggagtc ccatcaaggt tcagtggcag tgggtctgga ac 52

20 <210> 74 <211> 52 <212> DNA <213> Artificial sequence

25 <220> <223> Synthetic primer

<220> <223> 194-LR7 30 <400> 74 caggttggca atggtgagag aataatctgt tccagaccca ctgccactga ac 52

35 <210> 75 <211> 50

<212> DNA <213> Artificial sequence

<220> 5 <223> Synthetic primer 2021201261

<220> <223> 194-LR6

10 <400> 75 gcaaaagtaa gtggcaatat cttctggttg caggttggca atggtgagag 50

15 <210> 76 <211> 51 <212> DNA <213> Artificial sequence

20 <220> <223> Synthetic primer

<220> <223> 194-LR5 25 <400> 76 gaacgtccac ggaagcgtat taccctgttg gcaaaagtaa gtggcaatat c 51

30 <210> 77 <211> 52 <212> DNA <213> Artificial sequence 35 <220> <223> Synthetic primer

<220> <223> 194-LR4

5 <400> 77 cgtttggtta ccagtttggt gcctccaccg aacgtccacg gaagcgtatt ac 2021201261

52

10 <210> 78 <211> 46 <212> DNA <213> Artificial sequence

15 <220> <223> Synthetic primer

<220> <223> 194-LR3 20 <400> 78 accaccgccc gagccaccgc caccccgttt ggttaccagt ttggtg 46

25 <210> 79 <211> 50 <212> DNA <213> Artificial sequence 30 <220> <223> Synthetic primer

<220> 35 <223> 194-LR2

<400> 79

gctagcgctc ccacctcctc cagatccacc accgcccgag ccaccgccac 50

5 <210> 80 <211> 42 2021201261

<212> DNA <213> Artificial sequence

10 <220> <223> Synthetic primer

<220> <223> 194-LH-LR1 15 <400> 80 gttgcagctg gacctcgcta gcgctcccac ctcctccaga tc 42

20 <210> 81 <211> 54 <212> DNA <213> Artificial sequence 25 <220> <223> Synthetic primer

<220> 30 <223> 194-LH-HF1

<400> 81 gatctggagg aggtgggagc gctagcgagg tccagctgca acagtctgga cctg 54 35

<210> 82

<211> 50 <212> DNA <213> Artificial sequence

5 <220> <223> Synthetic primer 2021201261

<220> <223> 194-HF2 10 <400> 82 agctgcaaca gtctggacct gaactggtga agcctggagc ttcaatgaag 50

15 <210> 83 <211> 50 <212> DNA <213> Artificial sequence 20 <220> <223> Synthetic primer

<220> 25 <223> 194-HF3

<400> 83 agcctggagc ttcaatgaag atttcctgca aggcctctgg ttactcattc 50 30

<210> 84 <211> 53 <212> DNA 35 <213> Artificial sequence

<220>

<223> Synthetic primer

<220> <223> 194-HF4 5 <400> 84 2021201261

gcaaggcctc tggttactca ttcactggct acatcgtgaa ctggctgaag cag 53

10 <210> 85 <211> 52 <212> DNA <213> Artificial sequence 15 <220> <223> Synthetic primer

<220> 20 <223> 194-HF5

<400> 85 atcgtgaact ggctgaagca gagccatgga aagaaccttg agtggattgg ac 52 25

<210> 86 <211> 52 <212> DNA 30 <213> Artificial sequence

<220> <223> Synthetic primer

35 <220> <223> 194-HF6

<400> 86 gaaccttgag tggattggac ttattaatcc atacaaaggt cttactacct ac 52

5 <210> 87 2021201261

<211> 52 <212> DNA <213> Artificial sequence 10 <220> <223> Synthetic primer

<220> 15 <223> 194-HR6

<400> 87 aatgtggcct tgcccttgaa tttctggttg taggtagtaa gacctttgta tg 52 20

<210> 88 <211> 52 <212> DNA 25 <213> Artificial sequence

<220> <223> Synthetic primer

30 <220> <223> 194-HR5

<400> 88 catgtaggct gtgctggatg acttgtctac agttaatgtg gccttgccct tg 35 52

<210> 89 <211> 55 <212> DNA <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic primer

<220> 10 <223> 194-HR4

<400> 89 actgcagagt cttcagatgt cagactgagg agctccatgt aggctgtgct ggatg 55 15

<210> 90 <211> 52 <212> DNA 20 <213> Artificial sequence

<220> <223> Synthetic primer

25 <220> <223> 194-HR3

<400> 90 accatagtac ccagatcttg cacagtaata gactgcagag tcttcagatg tc 30 52

<210> 91 <211> 52 35 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer

<220> 5 <223> 194-HR2 2021201261

<400> 91 gcgccccaga catcgaagta ccagtccgag tcaccatagt acccagatct tg 52 10

<210> 92 <211> 52 <212> DNA 15 <213> Artificial sequence

<220> <223> Synthetic primer

20 <220> <223> 194-LH-HR1

<400> 92 gcgaatactc gaggagacgg tgaccgtggt ccctgcgccc cagacatcga ag 25 52

<210> 93 <211> 42 30 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 35 <220> <223> 194-HL-HF1

<400> 93 gcgtatgaac cggtgaggtc cagctgcaac agtctggacc tg 42 5 2021201261

<210> 94 <211> 55 <212> DNA 10 <213> Artificial sequence

<220> <223> Synthetic primer

15 <220> <223> 194-HL-HR1

<400> 94 accgccacca gaggagacgg tgaccgtggt ccctgcgccc cagacatcga 20 agtac 55

<210> 95 <211> 52 25 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 30 <220> <223> 194-HL-HR0

<400> 95 35 acctcctcca gatccaccac cgcccgagcc accgccacca gaggagacgg tg

<210> 96 <211> 49 <212> DNA 5 <213> Artificial sequence 2021201261

<220> <223> Synthetic primer

10 <220> <223> 194-HL-LF1

<400> 96 gcgggggagg tggcagtgct agcgacatcc agatgacaca gactacatc 15 49

<210> 97 <211> 34 20 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 25 <220> <223> 194-HL-LR3Xho

<400> 97 30 gcgaatactc gagcgtttgg ttaccagttt ggtg 34

<210> 98 35 <211> 37 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer

5 <220> <223> 194-HL-LR3Xba 2021201261

<400> 98 gcgatatcta gattaccgtt tggttaccag tttggtg 10 37

<210> 99 <211> 43 15 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 20 <220> <223> 194-HL-HF1R1

<400> 99 25 gcgtatgaga attcagaggt ccagctgcaa cagtctggac ctg 43

<210> 100 30 <211> 41 <212> DNA <213> Artificial sequence

<220> 35 <223> Synthetic primer

<220>

<223> 194-LH-LF1R1

<400> 100 gcgtatgaga attctgacat ccagatgaca cagactacat c 5 41 2021201261

<210> 101 <211> 54 10 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 15 <220> <223> 194-LH-HR1Xba

<400> 101 20 gcgtatctag attaggagac ggtgaccgtg gtccctgcgc cccagacatc gaag 54

<210> 102 25 <211> 725 <212> DNA <213> Artificial sequence

<220> 30 <223> Synthetic polynucleotide

<220> <223> G28-1 VLVH (DNA)

35 <400> 102 accggtgaca tccagatgac tcagtctcca gcctccctat ctgcatctgt gggagagact 60

gtcaccatca catgtcgaac aagtgaaaat gtttacagtt atttggcttg gtatcagcag 120

5 aaacagggaa aatctcctca gctcctggtc tcttttgcaa aaaccttagc agaaggtgtg 180 2021201261

ccatcaaggt tcagtggcag tggatcaggc acacagtttt ctctgaagat cagcagcctg 240 10 cagcctgaag attctggaag ttatttctgt caacatcatt ccgataatcc gtggacgttc 300

ggtggaggca ccgaactgga gatcaaaggt ggcggtggct cgggcggtgg 15 tgggtcgggt 360

ggcggcggat ctgctagcgc agtccagctg cagcagtctg gacctgagct ggaaaagcct 420

20 ggcgcttcag tgaagatttc ctgcaaggct tctggttact cattcactgg ctacaatatg 480

aactgggtga agcagaataa tggaaagagc cttgagtgga ttggaaatat tgatccttat 540 25 tatggtggta ctacctacaa ccggaagttc aagggcaagg ccacattgac tgtagacaaa 600

tcctccagca cagcctacat gcagctcaag agtctgacat ctgaggactc 30 tgcagtctat 660

tactgtgcaa gatcggtcgg ccctatggac tactggggtc aaggaacctc agtcaccgtc 720

35 tcgag

<210> 103 <211> 239 <212> PRT 5 <213> Artificial sequence 2021201261

<220> <223> Synthetic polypeptide

10 <220> <223> G-28-1 VLVH (AA)

<220> <221> misc_feature 15 <222> (1)..(107) <223> VL

<220> <221> misc_feature 20 <222> (108)..(124) <223> Linker

<220> <221> misc_feature 25 <222> (125)..(239) <223> VH

<400> 103

30 Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser Val Gly 1 5 10 15

35 Glu Thr Val Thr Ile Thr Cys Arg Thr Ser Glu Asn Val Tyr Ser Tyr 20 25 30

Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val 5 35 40 45 2021201261

Ser Phe Ala Lys Thr Leu Ala Glu Gly Val Pro Ser Arg Phe Ser Gly 10 50 55 60

Ser Gly Ser Gly Thr Gln Phe Ser Leu Lys Ile Ser Ser Leu Gln Pro 15 65 70 75 80

Glu Asp Ser Gly Ser Tyr Phe Cys Gln His His Ser Asp Asn 20 Pro Trp 85 90 95

Thr Phe Gly Gly Gly Thr Glu Leu Glu Ile Lys Gly Gly Gly Gly Ser 25 100 105 110

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Ala Val Gln Leu 30 115 120 125

Gln Gln Ser Gly Pro Glu Leu Glu Lys Pro Gly Ala Ser Val Lys Ile 35 130 135 140

Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Asn Met Asn Trp 145 150 155 160 5 2021201261

Val Lys Gln Asn Asn Gly Lys Ser Leu Glu Trp Ile Gly Asn Ile Asp 165 170 10 175

Pro Tyr Tyr Gly Gly Thr Thr Tyr Asn Arg Lys Phe Lys Gly Lys Ala 15 180 185 190

Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln Leu Lys 20 195 200 205

Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ser Val 25 210 215 220

Gly Pro Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 30 225 230 235

<210> 104 <211> 767 35 <212> DNA <213> Artificial sequence

<220> <223> Synthetic polynucleotide

<220> 5 <223> G28-1 VHVL (DNA) 2021201261

<400> 104 accggtgagg tccagctgca acagtctgga cctgaactgg tgaagcctgg agcttcaatg 60 10 aagatttcct gcaaggcctc tggttactca ttcactggct acatcgtgaa ctggctgaag 120

cagagccatg gaaagaacct tgagtggatt ggacttatta atccatacaa 15 aggtcttact 180

acctacaacc agaaattcaa gggcaaggcc acattaactg tagacaagtc atccagcaca 240

20 gcctacatgg agctcctcag tctgacatct gaagactctg cagtctatta ctgtgcaaga 300

tctgggtact atggtgactc ggactggtac ttcgatgtct ggggcgcagg gaccacggtc 360 25 accgtctcct ctggtggcgg tggctcgggc ggtggtggat ctggaggagg tgggagcggg 420

ggaggtggca gtgctagcga catccagatg acacagacta catcctccct 30 gtctgcctct 480

ctgggagaca gagtcaccat cagttgcagg gcaagtcagg acattcgcaa ttatttaaac 540

35 tggtatcagc agaaaccaga tggaactgtt aaactcctga tctactacac atcaagatta 600

cactcaggag tcccatcaag gttcagtggc agtgggtctg gaacagatta ttctctcacc 660

attgccaacc tgcaaccaga agatattgcc acttactttt gccaacaggg 5 taatacgctt 720 2021201261

ccgtggacgt tcggtggagg caccaaactg gtaaccaaac gctcgag 767

10 <210> 105 <211> 253 <212> PRT <213> Artificial sequence 15 <220> <223> Synthetic polypeptide

<220> 20 <223> G28-1 VHVL (AA)

<220> <221> misc_feature <222> (1)..(121) 25 <223> VH

<220> <221> misc_feature <222> (122)..(144) 30 <223> Linker

<220> <221> misc_feature <222> (145)..(253) 35 <223> VL

<400> 105

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 1 5 10 15 5 2021201261

Ser Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 20 25 30 10

Ile Val Asn Trp Leu Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile 35 40 45 15

Gly Leu Ile Asn Pro Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys Phe 50 55 60 20

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 65 70 75 25 80

Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 30 85 90 95

Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp 35 100 105 110

Gly Ala Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 115 120 125

5 Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 2021201261

Ala Ser 130 135 140

10 Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 145 150 155 160 15

Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr 165 170 20 175

Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 25 180 185 190

Tyr Tyr Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 30 195 200 205

Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro 35 210 215 220

Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Trp 225 230 235 240 5 2021201261

Thr Phe Gly Gly Gly Thr Lys Leu Val Thr Lys Arg Ser 245 250

10 <210> 106 <211> 749 <212> DNA <213> Artificial sequence 15 <220> <223> Synthetic polynucleotide

<220> 20 <223> G19-4 VLVH (DNA)

<400> 106 accggtgaca tccagatgac acagactaca tcctccctgt ctgcctctct gggagacaga 60 25 gtcaccatca gttgcagggc aagtcaggac attcgcaatt atttaaactg gtatcagcag 120

aaaccagatg gaactgttaa actcctgatc tactacacat caagattaca 30 ctcaggagtc 180

ccatcaaggt tcagtggcag tgggtctgga acagattatt ctctcaccat tgccaacctg 240

35 caaccagaag atattgccac ttacttttgc caacagggta atacgcttcc gtggacgttc 300

ggtggaggca ccaaactggt aaccaaacgg ggtggcggtg gctcgggcgg tggtggatct 360

ggaggaggtg ggagcgctag cgaggtccag ctgcaacagt ctggacctga 5 actggtgaag 420 2021201261

cctggagctt caatgaagat ttcctgcaag gcctctggtt actcattcac tggctacatc 480

10 gtgaactggc tgaagcagag ccatggaaag aaccttgagt ggattggact tattaatcca 540

tacaaaggtc ttactaccta caaccagaaa ttcaagggca aggccacatt aactgtagac 600 15 aagtcatcca gcacagccta catggagctc ctcagtctga catctgaaga ctctgcagtc 660

tattactgtg caagatctgg gtactatggt gactcggact ggtacttcga 20 tgtctggggc 720

gcagggacca cggtcaccgt ctcctcgag 749

25 <210> 107 <211> 247 <212> PRT <213> Artificial sequence 30 <220> <223> Synthetic polypeptide

<220> 35 <223> G-19-4 VLVH (AA)

<220>

<221> misc_feature <222> (1)..(108) <223> VL

5 <220> <221> misc_feature 2021201261

<222> (109)..(125) <223> Linker

10 <220> <221> misc_feature <222> (126)..(247) <223> VH

15 <400> 107

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 1 5 10 15 20

Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr 20 25 30 25

Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 35 40 45 30

Tyr Tyr Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 35

Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ala Asn Leu

Gln Pro 65 70 75 80

5 Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu 2021201261

Pro Trp 85 90 95

10 Thr Phe Gly Gly Gly Thr Lys Leu Val Thr Lys Arg Gly Gly Gly Gly 100 105 110

15 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Glu Val Gln 115 120 125

20 Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Met Lys 130 135 140

25 Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Ile Val Asn 145 150 155 160 30

Trp Leu Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile Gly Leu Ile 165 170 35 175

Asn Pro Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys Phe Lys Gly Lys 180 185 190

5 Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met 2021201261

Glu Leu 195 200 205

10 Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ser 210 215 220

15 Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp Gly Ala Gly 225 230 235 240 20

Thr Thr Val Thr Val Ser Ser 245

25 <210> 108 <211> 752 <212> DNA <213> Artificial sequence 30 <220> <223> Synthetic polynucleotide

<220> 35 <223> G19-4 VHVL (DNA)

<400> 108

accggtgagg tccagctgca acagtctgga cctgaactgg tgaagcctgg agcttcaatg 60

aagatttcct gcaaggcctc tggttactca ttcactggct acatcgtgaa 5 ctggctgaag 120 2021201261

cagagccatg gaaagaacct tgagtggatt ggacttatta atccatacaa aggtcttact 180

10 acctacaacc agaaattcaa gggcaaggcc acattaactg tagacaagtc atccagcaca 240

gcctacatgg agctcctcag tctgacatct gaagactctg cagtctatta ctgtgcaaga 300 15 tctgggtact atggtgactc ggactggtac ttcgatgtct ggggcgcagg gaccacggtc 360

accgtctcct ctggtggcgg tggctcgggc ggtggtggat ctggaggagg 20 tgggagcgct 420

agcgacatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc 480

25 accatcagtt gcagggcaag tcaggacatt cgcaattatt taaactggta tcagcagaaa 540

ccagatggaa ctgttaaact cctgatctac tacacatcaa gattacactc aggagtccca 600 30 tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattgc caacctgcaa 660

ccagaagata ttgccactta cttttgccaa cagggtaata cgcttccgtg 35 gacgttcggt 720

ggaggcacca aactggtaac caaacgctcg ag

<210> 109 5 <211> 248 <212> PRT 2021201261

<213> Artificial sequence

<220> 10 <223> Synthetic polypeptide

<220> <223> G19-4 VHVL (AA)

15 <220> <221> misc_feature <222> (1)..(122) <223> VH

20 <220> <221> misc_feature <222> (123)..(139) <223> Linker

25 <220> <221> misc_feature <222> (140)..(248) <223> VL

30 <400> 109

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 1 5 10 15 35

Ser Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr

Gly Tyr 20 25 30

5 Ile Val Asn Trp Leu Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile 2021201261

35 40 45

10 Gly Leu Ile Asn Pro Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys Phe 50 55 60

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr 15 Ala Tyr 65 70 75 80

20 Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 85 90 95

25 Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp 100 105 110

30 Gly Ala Gly Thr Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 115 120 125

35 Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Asp Ile Gln Met Thr 130 135 140

Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile 5 145 150 155 160 2021201261

Ser Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr Leu Asn Trp 10 Tyr Gln 165 170 175

15 Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr Tyr Thr Ser Arg 180 185 190

20 Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 195 200 205

25 Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro Glu Asp Ile Ala Thr 210 215 220

30 Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Trp Thr Phe Gly Gly Gly 225 230 235 240

35 Thr Lys Leu Val Thr Lys Arg Ser

<210> 110 <211> 45 5 <212> DNA <213> Artificial sequence 2021201261

<220> <223> Synthetic primer 10 <220> <221> misc_feature <223> ssc(s)-hIgG1 (DNA)

15 <400> 110 gagcccaaat cttctgacaa aactcacaca tctccaccgt gctca 45

<210> 111 20 <211> 15 <212> PRT <213> Artificial sequence

<220> 25 <223> Synthetic peptide

<220> <221> misc_feature 30 <223> ssc(s)-hIgG1 (AA)

<400> 111

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Cys 35 Ser 1 5 10 15

<210> 112 <211> 45 <212> DNA 5 <213> Artificial sequence 2021201261

<220> <223> Synthetic primer

10 <220> <221> misc_feature <223> scc(s)-hIgG1 (DNA)

<400> 112 15 gagcccaaat cttctgacaa aactcacaca tgtccaccgt gctca 45

<210> 113 20 <211> 15 <212> PRT <213> Artificial sequence

<220> 25 <223> Synthetic peptide

<220> <221> misc_feature 30 <223> scc(s)-hIgG1 (AA)

<400> 113

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys 35 Ser 1 5 10 15

<210> 114 <211> 45 <212> DNA 5 <213> Artificial sequence 2021201261

<220> <223> Synthetic primer

10 <220> <221> misc_feature <223> css(s)-hIgG1 (DNA)

15 <400> 114 gagcccaaat cttgtgacaa aactcacaca tctccaccga gctca 45

20 <210> 115 <211> 15 <212> PRT <213> Artificial sequence

25 <220> <223> Synthetic peptide

<220> <221> misc_feature 30 <223> css(s)-hIgG1 (AA)

<400> 115

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Ser Pro Pro Ser 35 Ser 1 5 10 15

<210> 116 <211> 45 <212> DNA 5 <213> Artificial sequence 2021201261

<220> <223> Synthetic polynucleotide

10 <220> <221> misc_feature <223> scs(s)-hIgG1 (DNA)

15 <400> 116 gagcccaaat cttgtgacaa aactcacaca tgtccaccga gctca 45

20 <210> 117 <211> 15 <212> PRT <213> Artificial sequence

25 <220> <223> Synthetic peptide

<220> <221> misc_feature 30 <223> scs(s)-hIgG1 (AA)

<400> 117

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Ser 35 Ser 1 5 10 15

<210> 118 <211> 45 <212> DNA 5 <213> Artificial sequence 2021201261

<220> <223> Synthetic primer

10 <400> 118 gagcccaaat cttgtgacaa aactcacaca tgtccaccgt gctca 45

15 <210> 119 <211> 15 <212> PRT <213> Artificial sequence 20 <220> <223> Synthetic peptide

25 <400> 119

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Ser 1 5 10 15 30

<210> 120 <211> 45 <212> DNA 35 <213> Artificial sequence

<220>

<223> Synthetic primer

<400> 120 5 gagcccaaat cttgtgacaa aactcacaca tgtccaccgt gccca 45 2021201261

<210> 121 10 <211> 15 <212> PRT <213> Artificial sequence

<220> 15 <223> Synthetic peptide

<400> 121

20 Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 1 5 10 15

25 <210> 122 <211> 45 <212> DNA <213> Artificial sequence

30 <220> <223> Synthetic primer

<400> 122 gagcccaaat cttctgacaa aactcacaca tctccaccga gccca 35 45

<210> 123 <211> 15 <212> PRT <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic peptide

<400> 123 10 Glu Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro 1 5 10 15

15 <210> 124 <211> 45 <212> DNA <213> Artificial sequence 20 <220> <223> Synthetic primer

<220> 25 <221> misc_feature <223> csc(p)-hIgG1 (DNA)

<400> 124 gagcccaaat cttgtgacaa aactcacaca tctccaccgt gccca 30 45

<210> 125 <211> 15 35 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide

5 <220> <221> misc_feature 2021201261

<223> csc(p)-hIgG1 (AA)

<400> 125 10 Glu Pro Lys Ser Cys Asp Lys Thr His Thr Ser Pro Pro Cys Pro 1 5 10 15

15 <210> 126 <211> 45 <212> DNA <213> Artificial sequence 20 <220> <223> Synthetic primer

25 <220> <221> misc_feature <223> ssc(p)-hIgG1 (DNA)

30 <400> 126 gagcccaaat cttctgacaa aactcacaca tctccaccgt gccca 45

35 <210> 127 <211> 15 <212> PRT

<213> Artificial sequence

<220> <223> Synthetic peptide 5 <220> 2021201261

<221> misc_feature <223> ssc(p)-hIgG1 (AA)

10 <400> 127

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Cys Pro 1 5 10 15 15

<210> 128 <211> 45 <212> DNA 20 <213> Artificial sequence

<220> <223> Synthetic primer

25 <220> <221> misc_feature <223> scc(p)-hIgG1 (DNA)

<400> 128 30 gagcccaaat cttctgacaa aactcacaca tgtccaccgt gccca 45

<210> 129 35 <211> 15 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide

5 <220> <221> misc_feature 2021201261

<223> scc(p)-hIgG1 (AA)

<400> 129 10 Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro 1 5 10 15

15 <210> 130 <211> 45 <212> DNA <213> Artificial sequence 20 <220> <223> Synthetic primer

<220> 25 <221> misc_feature <223> css(p)-hIgG1 (DNA)

<400> 130 gagcccaaat cttgtgacaa aactcacaca tctccaccga gccca 30 45

<210> 131 <211> 15 35 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide

<220> 5 <221> misc_feature <223> css(p)-hIgG1 (AA) 2021201261

<400> 131

10 Glu Pro Lys Ser Cys Asp Lys Thr His Thr Ser Pro Pro Ser Pro 1 5 10 15

15 <210> 132 <211> 45 <212> DNA <213> Artificial sequence

20 <220> <223> Synthetic primer

<220> <221> misc_feature 25 <223> ssc(p)-hIgG1 (DNA)

<400> 132 gagcccaaat cttgtgacaa aactcacaca tgtccaccga gccca 45 30

<210> 133 <211> 15 <212> PRT 35 <213> Artificial sequence

<220>

<223> Synthetic peptide

<220> <221> misc_feature 5 <223> ssc(p)-hIgG1 (DNA) 2021201261

<400> 133

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Ser 10 Pro 1 5 10 15

<210> 134 <211> 18 15 <212> DNA <213> Artificial sequence

<220> <221> misc_feature 20 <223> ssc(p) (DNA)

<220> <223> Synthetic primer

25 <400> 134 agttgtccac cgtgccca 18

30 <210> 135 <211> 6 <212> PRT <213> Artificial sequence

35 <220> <223> Synthetic peptide

<400> 135

Ser Cys Pro Pro Cys Pro 1 5 5 2021201261

<210> 136 <211> 7 <212> DNA 10 <213> Artificial sequence

<220> <223> Synthetic primer

15 <400> 136 tgatcag 7

20 <210> 137 <211> 2 <212> PRT <213> Artificial sequence

25 <220> <223> Synthetic peptide

<400> 137

30 Asp Gln 1

<210> 138 35 <211> 7 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer

5 <400> 138 ctcgagt 2021201261

7

10 <210> 139 <211> 2 <212> PRT <213> Artificial sequence

15 <220> <223> Synthetic peptide

<400> 139

20 Ser Ser 1

<210> 140 25 <211> 654 <212> DNA <213> Homo sapiens

<220> 30 <223> hIgG1 wild type

<400> 140 gcacctgaac tcctgggtgg accgtcagtc ttcctcttcc ccccaaaacc caaggacacc 60 35 ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac 120

cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag 180

5 ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac 240 2021201261

caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc 300 10 cccatcgaga aaacaatctc caaagccaaa gggcagcccc gagaaccaca ggtgtacacc 360

ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg 15 cctggtcaaa 420

ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac 480

20 tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc 540

accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt gatgcatgag 600 25 gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa atga 654

30 <210> 141 <211> 217 <212> PRT <213> Homo sapiens

35 <220> <223> hIgG1 wild type

<400> 141

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 5 1 5 10 15 2021201261

Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 10 20 25 30

Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 35 40 45 15

Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 50 55 60 20

Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 65 70 75 25 80

Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 30 85 90 95

Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 35 100 105 110

Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 115 120 125

5 Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 2021201261

Tyr Pro 130 135 140

10 Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 145 150 155 160 15

Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 165 170 20 175

Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 25 180 185 190

Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 30 195 200 205

Lys Ser Leu Ser Leu Ser Pro Gly Lys 210 215 35

<210> 142

<211> 654 <212> DNA <213> Homo sapiens

5 <220> <223> hIgG1 (P238S) 2021201261

<400> 142 gcacctgaac tcctgggtgg atcgtcagtc ttcctcttcc ccccaaaacc 10 caaggacacc 60

ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac 120

15 cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag 180

ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac 240 20 caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc 300

cccatcgaga aaacaatctc caaagccaaa gggcagcccc gagaaccaca 25 ggtgtacacc 360

ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg cctggtcaaa 420

30 ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc ggagaacaac 480

tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc 540 35 accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt gatgcatgag 600

gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa atga 654

5 <210> 143 2021201261

<211> 217 <212> PRT <213> Homo sapiens 10 <220> <223> hIgG1 (P238S)

<400> 143 15 Ala Pro Glu Leu Leu Gly Gly Ser Ser Val Phe Leu Phe Pro Pro Lys 1 5 10 15

20 Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 20 25 30

25 Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 35 40 45

30 Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 50 55 60

35 Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His

65 70 75 80

5 Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 2021201261

85 90 95

10 Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 100 105 110

15 Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 115 120 125

20 Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 130 135 140

25 Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 145 150 155 160

30 Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 165 170 175 35 Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val

180 185 190

Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 5 Thr Gln 195 200 205 2021201261

Lys Ser Leu Ser Leu Ser Pro Gly Lys 10 210 215

<210> 144 <211> 654 15 <212> DNA <213> Homo sapiens

<220> <223> hIgG1 (P331S) 20 <400> 144 gcacctgaac tcctgggtgg accgtcagtc ttcctcttcc ccccaaaacc caaggacacc 60

25 ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac 120

cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc caagacaaag 180 30 ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac 240

caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc 35 cctcccagcc 300

tccatcgaga aaacaatctc caaagccaaa gggcagcccc gagaaccaca

ggtgtacacc 360

ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg cctggtcaaa 420 5 ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc 2021201261

ggagaacaac 480

tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta 10 cagcaagctc 540

accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt gatgcatgag 600

15 gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa atga 654

<210> 145 20 <211> 217 <212> PRT <213> Homo sapiens

<220> 25 <223> hIgG1 (P331S)

<400> 145

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 30 Pro Lys 1 5 10 15

Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 35 Cys Val 20 25 30

Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 35 40 45 5 2021201261

Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 50 55 60 10 Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 65 70 75 80 15

Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 85 90 95 20

Ala Leu Pro Ala Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 100 105 110 25

Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 115 120 125 30

Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 130 135 140 35

Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu

Asn Asn 145 150 155 160

5 Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 2021201261

Phe Leu 165 170 175 10

Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 180 185 190 15

Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 195 200 205 20

Lys Ser Leu Ser Leu Ser Pro Gly Lys 210 215

25 <210> 146 <211> 654 <212> DNA <213> Homo sapiens 30 <220> <223> hIgG1 (P238S/P331S)

<400> 146 35 gcacctgaac tcctgggtgg atcgtcagtc ttcctcttcc ccccaaaacc caaggacacc 60

ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac 120

cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc 5 caagacaaag 180 2021201261

ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac 240

10 caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc 300

tccatcgaga aaacaatctc caaagccaaa gggcagcccc gagaaccaca ggtgtacacc 360 15 ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg cctggtcaaa 420

ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc 20 ggagaacaac 480

tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta cagcaagctc 540 accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt 25 gatgcatgag 600

gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa atga 654

30 <210> 147 <211> 217 <212> PRT <213> Homo sapiens 35 <220> <223> hIgG1 (P238S/P331S)

<400> 147

Ala Pro Glu Leu Leu Gly Gly Ser Ser Val Phe Leu Phe Pro 5 Pro Lys 1 5 10 15 2021201261

Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 10 Cys Val 20 25 30

Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 15 Trp Tyr 35 40 45

Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 20 Glu Glu 50 55 60

Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 25 Leu His 65 70 75 80

30 Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 85 90 95

35 Ala Leu Pro Ala Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 100 105 110

Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 5 115 120 125 2021201261

Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 10 130 135 140

Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 15 145 150 155 160

Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 20 Phe Leu 165 170 175

25 Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 180 185 190

30 Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 195 200 205

Lys Ser Leu Ser Leu Ser Pro Gly Lys 35 210 215

<210> 148 <211> 60 <212> DNA <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic primer

<220> 10 <223> STD1 (DNA)

<400> 148 aattatggtg gcggtggctc gggcggtggt ggatctggag gaggtgggag tgggaattct 60 15

<210> 149 <211> 20 <212> PRT 20 <213> Artificial sequence

<220> <223> Synthetic peptide

25 <220> <223> STD1 (AA)

<400> 149 30 Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 1 5 10 15

35 Ser Gly Asn Ser

<210> 150 <211> 114 5 <212> DNA <213> Artificial sequence 2021201261

<220> <223> Synthetic primer 10

<220> <223> STD2 (DNA)

15 <400> 150 aattatggtg gcggtggctc gggcggtggt ggatctggag gaggtgggag tgggaattat 60

ggtggcggtg gctcgggcgg tggtggatct ggaggaggtg ggagtgggaa 20 ttct 114

<210> 151 <211> 38 25 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 30

<220> <223> STD2 (AA)

35 <400> 151

Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly

Gly Gly 1 5 10 15

5 Ser Gly Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 2021201261

20 25 30

10 Gly Gly Ser Gly Asn Ser 35

<210> 152 15 <211> 6 <212> DNA <213> Artificial sequence

<220> 20 <223> Synthetic primer

<220> <223> Linker H1 (PN)

25 <400> 152 aattct 6

30 <210> 153 <211> 2 <212> PRT <213> Artificial sequence

35 <220> <223> Synthetic peptide

<220> <223> Linker H1 (AA)

5 <400> 153 2021201261

Asn Ser 1

10 <210> 154 <211> 24 <212> DNA <213> Artificial sequence 15 <220> <223> Synthetic primer

<220> 20 <223> Linker H2 (PN)

<400> 154 ggtggcggtg gctcggggaa ttct 24 25

<210> 155 <211> 8 <212> PRT 30 <213> Artificial sequence

<220> <223> Synthetic primer

35 <220> <223> Linker H2 (AA)

<400> 155

Gly Gly Gly Gly Ser Gly Asn Ser 5 1 5 2021201261

<210> 156 10 <211> 30 <212> DNA <213> Artificial sequence

<220> 15 <223> Synthetic primer

<220> <223> Linker H3 (PN)

20 <400> 156 aattatggtg gcggtggctc tgggaattct 30

25 <210> 157 <211> 10 <212> PRT <213> Artificial sequence

30 <220> <223> Synthetic peptide

<220> <223> Linker H3 (AA) 35 <400> 157

Asn Tyr Gly Gly Gly Gly Ser Gly Asn Ser 1 5 10

5 <210> 158 <211> 39 2021201261

<212> DNA <213> Artificial sequence

10 <220> <223> Synthetic primer

<220> <223> Linker H4 (PN) 15 <400> 158 ggtggcggtg gctcgggcgg tggtggatct gggaattct 39

20 <210> 159 <211> 13 <212> PRT <213> Artificial sequence 25 <220> <223> Synthetic peptide

<220> 30 <223> Linker H4 (AA)

<400> 159

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Asn Ser 35 1 5 10

<210> 160 <211> 45 <212> DNA <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic primer

<220> 10 <223> Linker H5 (PN)

<400> 160 aattatggtg gcggtggctc gggcggtggt ggatctggga attct 45 15

<210> 161 <211> 15 <212> PRT 20 <213> Artificial sequence

<220> <223> Synthetic peptide

25 <220> <223> Linker H5 (AA)

<400> 161

30 Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Asn Ser 1 5 10 15

35 <210> 162 <211> 54 <212> DNA

<213> Artificial sequence

<220> <223> Synthetic primer 5 <220> 2021201261

<223> Linker H6 (PN)

<400> 162 10 ggtggcggtg gctcgggcgg tggtggatct gggggaggag gcagcgggaa ttct 54

<210> 163 15 <211> 18 <212> PRT <213> Artificial sequence

<220> 20 <223> Synthetic peptide

<220> <223> Linker H6 (AA)

25 <400> 163

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 1 5 10 15 30

Asn Ser

35 <210> 164 <211> 24

<212> DNA <213> Artificial sequence

<220> 5 <223> Synthetic primer 2021201261

<220> <223> Linker H7 (PN)

10 <400> 164 gggtgtccac cttgtccgaa ttct 24

15 <210> 165 <211> 8 <212> PRT <213> Artificial sequence

20 <220> <223> Synthetic peptide

<220> <223> Linker H7 (AA) 25 <400> 165

Gly Cys Pro Pro Cys Pro Asn Ser 1 5 30

<210> 166 <211> 45 <212> DNA 35 <213> Artificial sequence

<220>

<223> Synthetic primer

<220> <223> Linker(G4S)3 5 <400> 166 2021201261

ggtggcggtg gatccggcgg aggtgggtcg ggtggcggcg gatct 45

10 <210> 167 <211> 12 <212> PRT <213> Artificial sequence 15 <220> <223> Synthetic peptide

<220> 20 <223> Linker(G4S)3

<400> 167

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser 25 1 5 10

<210> 168 <211> 60 30 <212> DNA <213> Artificial sequence

<220> <223> Synthetic primer 35 <220> <223> Linker(G4S)4

<400> 168 ggtggcggtg gctcgggcgg tggtggatct ggaggaggtg ggagcggggg aggtggcagt 60 5 2021201261

<210> 169 <211> 17 <212> PRT 10 <213> Artificial sequence

<220> <223> Synthetic peptide

15 <220> <223> Linker(G4S)4

<400> 169

20 Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly 1 5 10 15

25 Ser

<210> 170 <211> 2337 <212> DNA 30 <213> Artificial sequence

<220> <223> Synthetic polynucleotide

35 <220> <223> 2H7sssIgG1-STD1-2e12HL (DNA)

<400> 170 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60

5 gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 2021201261

ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 10 taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240

tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc 15 tctcacaatc 300

agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360

20 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420

ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg 480 25 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540

aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg 30 agctatttat 600

ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660

35 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720

gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga 5 caaaactcac 840 2021201261

acatccccac cgagctcagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900

10 ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960

gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020 15 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg 20 caaggtctcc 1140

aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga 1200

25 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260

ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320 30 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380

ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa 35 cgtcttctca 1440

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct

ctccctgtct 1500

ccgggtaaga attatggtgg cggtggctcg ggcggtggtg gatctggagg aggtgggagt 1560 5 gggaattctc aggtgcagct gaaggagtca ggacctggcc tggtggcgcc 2021201261

ctcacagagc 1620

ctgtccatca catgcaccgt ctcagggttc tcattaaccg gctatggtgt 10 aaactgggtt 1680

cgccagcctc caggaaaggg tctggagtgg ctgggaatga tatggggtga tggaagcaca 1740

15 gactataatt cagctctcaa atccagacta tcgatcacca aggacaactc caagagccaa 1800

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccagatacta ctgtgctcga 1860 20 gatggttata gtaactttca ttactatgtt atggactact ggggtcaagg aacctcagtc 1920

accgtctcct ctgggggtgg aggctctggt ggcggtggat ccggcggagg 25 tgggtcgggt 1980

ggcggcggat ctgacattgt gctcacccaa tctccagctt ctttggctgt gtctctaggt 2040

30 cagagagcca ccatctcctg cagagccagt gaaagtgttg aatattatgt cacaagttta 2100

atgcagtggt accaacagaa accaggacag ccacccaaac tcctcatctc tgctgctagc 2160 35 aacgtagaat ctggggtccc tgccaggttt agtggcagtg ggtctgggac agactttagc 2220

ctcaacatcc atcctgtgga ggaggatgat attgcaatgt atttctgtca gcaaagtagg 2280

5 aaggttccat ggacgttcgg tggaggcacc aagctggaaa tcaaacgtta atctaga 2337 2021201261

<210> 171 10 <211> 772 <212> PRT <213> Artificial sequence

<220> 15 <223> Synthetic polypeptide

<220> <223> 2H7sssIgG1-STD1-2e12HL (AA)

20 <220> <221> misc_feature <222> (1)..(22) <223> Leader

25 <220> <221> misc_feature <222> (23)..(128) <223> VL

30 <220> <221> misc_feature <222> (129)..(144) <223> Linker

35 <220> <221> misc_feature <222> (145)..(265)

<223> VH

<220> <221> misc_feature 5 <222> (268)..(281) <223> Hinge 2021201261

<220> <221> misc_feature 10 <222> (500)..(519) <223> EFD-BD2 linker

<220> <221> misc_feature 15 <222> (520)..(640) <223> VH2

<220> <221> misc_feature 20 <222> (641)..(660) <223> Linker2

<220> <221> misc_feature 25 <222> (661)..(772) <223> VL2

<400> 171

30 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15

35 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 5 35 40 45 2021201261

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 10 50 55 60

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 15 65 70 75 80

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu 20 Thr Ile 85 90 95

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 25 Gln Trp 100 105 110

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu 30 Leu Lys 115 120 125

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 35 Ser Ser 130 135 140

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 5 160 2021201261

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 10 165 170 175

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu 15 Trp Ile 180 185 190

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln 20 Lys Phe 195 200 205

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr 25 Ala Tyr 210 215 220

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 30 Phe Cys 225 230 235 240

35 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 245 250

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 5 260 265 270 2021201261

Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala Pro Glu Leu Leu Gly 10 275 280 285

Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 15 290 295 300

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 20 305 310 315 320

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 25 Glu Val 325 330 335

30 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350

35 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355 360 365

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 5 370 375 380 2021201261

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 10 385 390 395 400

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 15 Val Ser 405 410 415

20 Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430

25 Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445

30 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460

35 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 5 Leu Ser 485 490 2021201261

495

10 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 500 505 510

Gly Gly Gly Ser Gly Asn Ser Gln Val Gln Leu Lys Glu Ser 15 Gly Pro 515 520 525

Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr 20 Val Ser 530 535 540

Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln 25 Pro Pro 545 550 555 560

30 Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 565 570 575

35 Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn

580 585 590

Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr 5 Asp Asp 595 600 605 2021201261

Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe 10 His Tyr 610 615 620

Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 15 Ser Ser 625 630 635 640

20 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 645 650 655

25 Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala 660 665 670

30 Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser 675 680 685

35 Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro

690 695 700

Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val 5 Glu Ser 705 710 715 2021201261

720

10 Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 725 730 735

15 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys 740 745 750

20 Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 755 760 765

25 Glu Ile Lys Arg 770

<210> 172 30 <211> 772 <212> PRT <213> Artificial sequence

<220> 35 <223> Synthetic polypeptide

<220>

<223> 2H7sssIgG1(P238S/P331S)-STD1-2e12HL (w/2e12 leader) (AA)

<220> 5 <221> misc_feature <222> (1)..(22) 2021201261

<223> Leader

<220> 10 <221> misc_feature <222> (23)..(128) <223> VL

<220> 15 <221> misc_feature <222> (129)..(144) <223> Linker

<220> 20 <221> misc_feature <222> (145)..(265) <223> VH

<220> 25 <221> misc_feature <222> (268)..(282) <223> Hinge

<220> 30 <221> misc_feature <222> (500)..(519) <223> EFD-BD2 linker

<220> 35 <221> misc_feature <222> (520)..(640) <223> VH2

<220> <221> misc_feature <222> (641)..(660) 5 <223> Linker2 2021201261

<220> <221> misc_feature <222> (661)..(772) 10 <223> VL2

<400> 172

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser 15 Ala Ser 1 5 10 15

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro 20 Ala Ile 20 25 30

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg 25 Ala Ser 35 40 45

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly 30 Ser Ser 50 55 60

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly 35 Val Pro 65 70 75

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 5 85 90 95 2021201261

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 10 100 105 110

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 15 115 120 125

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 20 130 135 140

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 25 145 150 155 160

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 30 Ser Tyr 165 170 175

35 Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 5 2021201261

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 10

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230 235 15 240

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 20 245 250 255

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys 25 Ser Ser 260 265 270

Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala Pro Glu Leu 30 Leu Gly 275 280 285

Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 35 Leu Met 290 295 300

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 5 320 2021201261

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 10 325 330 335

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 15 Thr Tyr 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 20 Asn Gly 355 360 365

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 25 Ser Ile 370 375 380

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 30 Gln Val 385 390 395 400

35 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 5 Val Glu 420 425 430 2021201261

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 10 Pro Pro 435 440 445

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 15 450 455 460

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 20 465 470 475 480

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 25 Leu Ser 485 490 495

30 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 500 505 510

35 Gly Gly Gly Ser Gly Asn Ser Gln Val Gln Leu Lys Glu Ser Gly Pro 515 520 525

Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser 5 530 535 540 2021201261

Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro 10 545 550 555 560

Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly 15 Ser Thr 565 570 575

20 Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 580 585 590

25 Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 595 600 605

30 Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr 610 615 620

35 Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 625 630 635

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 5 Ser Gly 645 650 2021201261

655

10 Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala 660 665 670

15 Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser 675 680 685

20 Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 690 695 700

Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val 25 Glu Ser 705 710 715 720

30 Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 725 730 735

35 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys

740 745 750

Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr 5 Lys Leu 755 760 765 2021201261

Glu Ile Lys Arg 10 770

<210> 173 <211> 2322 15 <212> DNA <213> Artificial sequence

<220> <223> Synthetic polynucleotide 20 <220> <223> 2H7sssIgG1-STD1-2e12LH (DNA)

<400> 173 25 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60

gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 30 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180

taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc 35 caacctggct 240

tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc

tctcacaatc 300

agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360 5 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc 2021201261

gggcggtggt 420

ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc 10 tgagtcggtg 480

aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540

15 aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600

ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660 20 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720

gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt 25 cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac 840

30 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960 35 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080

5 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140 2021201261

aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga 1200 10 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260

ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg 15 ggagagcaat 1320

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380

20 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500 25 ccgggtaaga attatggtgg cggtggctcg ggcggtggtg gatctggagg aggtgggagt 1560

gggaattctg acattgtgct cacccaatct ccagcttctt tggctgtgtc 30 tctaggtcag 1620

agagccacca tctcctgcag agccagtgaa agtgttgaat attatgtcac aagtttaatg 1680

35 cagtggtacc aacagaaacc aggacagcca cccaaactcc tcatctctgc tgctagcaac 1740

gtagaatctg gggtccctgc caggtttagt ggcagtgggt ctgggacaga ctttagcctc 1800

aacatccatc ctgtggagga ggatgatatt gcaatgtatt tctgtcagca 5 aagtaggaag 1860 2021201261

gttccatgga cgttcggtgg aggcaccaag ctggaaatca aacggggtgg cggtggatcc 1920

10 ggcggaggtg ggtcgggtgg cggcggatct caggtgcagc tgaaggagtc aggacctggc 1980

ctggtggcgc cctcacagag cctgtccatc acatgcaccg tctcagggtt ctcattaacc 2040 15 ggctatggtg taaactgggt tcgccagcct ccaggaaagg gtctggagtg gctgggaatg 2100

atatggggtg atggaagcac agactataat tcagctctca aatccagact 20 atcgatcacc 2160

aaggacaact ccaagagcca agttttctta aaaatgaaca gtctgcaaac tgatgacaca 2220

25 gccagatact actgtgctcg agatggttat agtaactttc attactatgt tatggactac 2280

tggggtcaag gaacctcagt caccgtctcc tcttaatcta ga 2322 30

<210> 174 <211> 767 <212> PRT 35 <213> Artificial sequence

<220>

<223> Synthetic polypeptide

<220> <223> 2H7sssIgG1-STD1-2e12LH (w/2e12 leader) (AA) 5 <220> 2021201261

<221> misc_feature <222> (1)..(22) <223> Leader 10 <220> <221> misc_feature <222> (23)..(128) <223> VL 15 <220> <221> misc_feature <222> (129)..(144) <223> Linker 20 <220> <221> misc_feature <222> (145)..(265) <223> VH 25 <220> <221> misc_feature <222> (268)..(282) <223> Hinge 30 <220> <221> misc_feature <222> (500)..(519) <223> EFD-BD2 linker 35 <220> <221> misc_feature

<222> (520)..(631) <223> VL2

<220> 5 <221> misc_feature <222> (632)..(646) 2021201261

<223> Linker2

<220> 10 <221> misc_feature <222> (647)..(767) <223> VH2

<400> 174 15 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15

20 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30

25 Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45

30 Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60

35 Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro

65 70 75 80

5 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 2021201261

85 90 95

10 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105 110

15 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 115 120 125

20 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140

25 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160

30 Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 35

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu

Trp Ile 180 185 190

5 Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 2021201261

195 200 205

10 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220

15 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230 235 240

20 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 245 250 255 25

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 30

Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 35

Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr

Leu Met 290 295 300

5 Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 2021201261

305 310 315 320

10 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335 15

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350 20

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355 360 365 25

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 370 375 380 30

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 35 400

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 5 2021201261

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 10

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 15

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 20

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475 25 480

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 30 485 490 495

Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly 35 Ser Gly 500 505 510

Gly Gly Gly Ser Gly Asn Ser Asp Ile Val Leu Thr Gln Ser Pro Ala 515 520 525

5 Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys 2021201261

Arg Ala 530 535 540

10 Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln 545 550 555 560 15

Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 565 570 20 575

Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 25 580 585 590

Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met 30 595 600 605

Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly 35 610 615 620

Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly 625 630 635 640 5 2021201261

Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly 645 650 10 655

Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly 15 660 665 670

Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly 20 675 680 685

Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp 25 690 695 700

Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser 30 705 710 715 720

Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp 35 Asp Thr 725 730

Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr 5 740 745 750 2021201261

Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 10 755 760 765

<210> 175 <211> 767 <212> PRT 15 <213> Artificial sequence

<220> <223> Synthetic polypeptide

20 <220> <223> 2H7sssIgG1(P238S/P331S)-STD1-2e12LH (w/2e12 leader) (AA)

<220> 25 <221> misc_feature <222> (1)..(22) <223> Leader

<220> 30 <221> misc_feature <222> (23)..(128) <223> VL

<220> 35 <221> misc_feature <222> (129)..(144) <223> Linker

<220> <221> misc_feature <222> (145)..(265) 5 <223> VH 2021201261

<220> <221> misc_feature <222> (268)..(282) 10 <223> Hinge

<220> <221> misc_feature <222> (500)..(519) 15 <223> EFD-BD2 Linker

<220> <221> misc_feature <222> (520)..(631) 20 <223> VL2

<220> <221> misc_feature <222> (632)..(646) 25 <223> Linker2

<220> <221> misc_feature <222> (647)..(767) 30 <223> VH2

<400> 175

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser 35 Ala Ser 1 5 10 15

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 5 2021201261

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 10

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 15

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 20 80

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 25 85 90 95

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 30 100 105 110

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 35 115 120 125

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140

5 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro 2021201261

Gly Ala 145 150 155 160 10

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 15 175

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 20 180 185 190

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 25 195 200 205

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 30

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230 235 35 240

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 245 250 255 5 2021201261

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 10

Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285 15

Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 20

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 25 320

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 30 325 330 335

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 35 Thr Tyr 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355 360 365 5 2021201261

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Ser Ile 370 375 380 10

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 15 400

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 20 405 410 415

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 25 Val Glu 420 425 430

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 30 Pro Pro 435 440 445

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 35 Thr Val 450 455 460

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475 480 5 2021201261

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 485 490 10 495

Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 15 500 505 510

Gly Gly Gly Ser Gly Asn Ser Asp Ile Val Leu Thr Gln Ser Pro Ala 20 515 520 525

Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala 25 530 535 540

Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln 30 545 550 555 560

Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala 35 Ser Asn 565 570

Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 5 580 585 590 2021201261

Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met 10 595 600 605

Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly 15 610 615 620

Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly 20 625 630 635 640

Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Lys Glu Ser Gly 25 Pro Gly 645 650 655

30 Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly 660 665 670

35 Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly 675 680 685

Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp 5 690 695 700 2021201261

Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser 10 705 710 715 720

Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 15 725 730 735

Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His 20 Tyr Tyr 740 745 750

Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 25 Ser 755 760 765

<210> 176 30 <211> 2376 <212> DNA <213> Artificial sequence

<220> 35 <223> Synthetic polynucleotide

<220>

<223> 2H7sssIgG1-STD2-2e12LH (DNA)

<400> 176 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat 5 cagtgcttca 60 2021201261

gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120

10 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180

taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240 15 tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300

agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag 20 ttttaaccca 360

cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420

25 ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg 480

aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540 30 aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600

ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac 35 actgactgta 660

gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga

agactctgcg 720

gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780 5 ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga 2021201261

caaaactcac 840

acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt 10 cctcttcccc 900

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960

15 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 20 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140

aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg 25 gcagccccga 1200

gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260

30 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380 35 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500

5 ccgggtaaga attatggtgg cggtggctcg ggcggtggtg gatctggagg aggtgggagt 1560 2021201261

gggaattatg gtggcggtgg ctcgggcggt ggtggatctg gaggaggtgg gagtgggaat 1620 10 tctgacattg tgctcaccca atctccagct tctttggctg tgtctctagg tcagagagcc 1680

accatctcct gcagagccag tgaaagtgtt gaatattatg tcacaagttt 15 aatgcagtgg 1740

taccaacaga aaccaggaca gccacccaaa ctcctcatct ctgctgctag caacgtagaa 1800

20 tctggggtcc ctgccaggtt tagtggcagt gggtctggga cagactttag cctcaacatc 1860

catcctgtgg aggaggatga tattgcaatg tatttctgtc agcaaagtag gaaggttcca 1920 25 tggacgttcg gtggaggcac caagctggaa atcaaacggg gtggcggtgg atccggcgga 1980

ggtgggtcgg gtggcggcgg atctcaggtg cagctgaagg agtcaggacc 30 tggcctggtg 2040

gcgccctcac agagcctgtc catcacatgc accgtctcag ggttctcatt aaccggctat 2100

35 ggtgtaaact gggttcgcca gcctccagga aagggtctgg agtggctggg aatgatatgg 2160

ggtgatggaa gcacagacta taattcagct ctcaaatcca gactatcgat caccaaggac 2220

aactccaaga gccaagtttt cttaaaaatg aacagtctgc aaactgatga 5 cacagccaga 2280 2021201261

tactactgtg ctcgagatgg ttatagtaac tttcattact atgttatgga ctactggggt 2340

10 caaggaacct cagtcaccgt ctcctcttaa tctaga 2376

<210> 177 15 <211> 785 <212> PRT <213> Artificial sequence

<220> 20 <223> Synthetic polypeptide

<220> <223> 2H7sssIgG1-STD2-2e12LH (w/2e12 leader)

25 <220> <221> misc_feature <222> (1)..(22) <223> Leader

30 <220> <221> misc_feature <222> (23)..(129) <223> VL

35 <220> <221> misc_feature <222> (130)..(144)

<223> Linker

<220> <221> misc_feature 5 <222> (145)..(265) <223> VH 2021201261

<220> <221> misc_feature 10 <222> (268)..(282) <223> Hinge

<220> <221> misc_feature 15 <222> (500)..(537) <223> EFD-BD2 Linker

<220> <221> misc_feature 20 <222> (538)..(649) <223> VL2

<220> <221> misc_feature 25 <222> (650)..(664) <223> Linker2

<220> <221> misc_feature 30 <222> (665)..(785) <223> VH2

<400> 177

35 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 5 20 25 30 2021201261

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 10

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 15

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 20 80

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 25 85 90 95

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 30 100 105 110

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 35 115 120 125

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140

5 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro 2021201261

Gly Ala 145 150 155 160 10

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 15 175

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 20 180 185 190

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 25 195 200 205

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 30 210 215 220

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 35 225 230 235

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 245 250 5 255 2021201261

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 10 260 265 270

Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala Pro Glu Leu Leu Gly 15 275 280 285

Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 20

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 25 320

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 30 325 330 335

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 35 Thr Tyr 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355 360 365 5 2021201261

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 370 375 380 10

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 15 400

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 20 405 410 415

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 25 Val Glu 420 425 430

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 30 Pro Pro 435 440 445

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 35 Thr Val 450 455 460

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475 5 480 2021201261

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 10 485 490 495

Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly 15 Ser Gly 500 505 510

Gly Gly Gly Ser Gly Asn Tyr Gly Gly Gly Gly Ser Gly Gly 20 Gly Gly 515 520 525

Ser Gly Gly Gly Gly Ser Gly Asn Ser Asp Ile Val Leu Thr 25 Gln Ser 530 535 540

Pro Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys 30 545 550 555 560

Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met 35 Gln Trp 565 570

Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala 5 580 585 590 2021201261

Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser 10 595 600 605

Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile 15 610 615 620

Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly 20 625 630 635 640

Gly Gly Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser 25 Gly Gly 645 650 655

30 Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Lys Glu Ser Gly 660 665 670

35 Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val 675 680 685

Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro 5 690 695 700 2021201261

Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser 10 705 710 715 720

Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr 15 Lys Asp 725 730 735

20 Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp 740 745 750

25 Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His 755 760 765

30 Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 770 775 780

35 Ser

<210> 178 <211> 785 <212> PRT <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic polypeptide

<220> 10 <223> 2H7sssIgG1(P238S/P331S)-STD2-2e12LH (w/2e12 leader) (AA)

<220> <221> misc_feature 15 <222> (1)..(22) <223> Leader

<220> <221> misc_feature 20 <222> (23)..(128) <223> VL

<220> <221> misc_feature 25 <222> (129)..(144) <223> Linker

<220> <221> misc_feature 30 <222> (145)..(265) <223> VH

<220> <221> misc_feature 35 <222> (268)..(281) <223> Hinge

<220> <221> misc_feature <222> (500)..(537) <223> EFD-BD2 Linker 5 <220> 2021201261

<221> misc_feature <222> (538)..(649) <223> VL2 10 <220> <221> misc_feature <222> (650)..(664) <223> Linker2 15 <220> <221> misc_feature <222> (665)..(785) <223> VH2 20 <400> 178

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 25 1 5 10 15

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 30 20 25 30

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 35 40 45

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60

5 Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly 2021201261

Val Pro 65 70 75 80 10

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95 15

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105 110 20

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 115 120 125 25

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140 30

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 35 160

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175 5 2021201261

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 10

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 15 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220

20 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230 235 240 25

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 245 250 30 255

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 35 260 265 270

Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285

5 Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 2021201261

Leu Met 290 295 300

10 Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 15

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 20 335

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 25 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 30 355 360 365

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Ser Ile 35 370 375 380

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400 5 2021201261

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 10 415

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 15 420 425 430

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 20 435 440 445

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 25 450 455 460

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475 30 480

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 35 485 490

Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 500 505 510 5 2021201261

Gly Gly Gly Ser Gly Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly 515 520 525 10

Ser Gly Gly Gly Gly Ser Gly Asn Ser Asp Ile Val Leu Thr Gln Ser 530 535 540 15

Pro Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys 545 550 555 20 560

Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp 25 565 570 575

Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser 30 Ala Ala 580 585 590

Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser 35 Gly Ser 595 600 605

Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile 610 615 620 5 2021201261

Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly 625 630 635 10 640

Gly Gly Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly 15 645 650 655

Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Lys Glu 20 Ser Gly 660 665 670

Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys 25 Thr Val 675 680 685

Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg 30 Gln Pro 690 695 700

Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp 35 Gly Ser 705 710 715

Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp 725 730 5 735 2021201261

Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp 10 740 745 750

Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His 15 755 760 765

Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 20 770 775 780

Ser 785 25

<210> 179 <211> 2391 <212> DNA 30 <213> Artificial sequence

<220> <223> Synthetic polynucleotide

35 <220> <223> 2H7sssIgG1-STD2-2e12HL (DNA)

<400> 179 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60

5 gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 2021201261

ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 10 taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240

tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc 15 tctcacaatc 300

agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360

20 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420

ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg 480 25 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540

aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg 30 agctatttat 600

ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660

35 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720

gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga 5 caaaactcac 840 2021201261

acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900

10 ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960

gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020 15 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg 20 caaggtctcc 1140

aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga 1200

25 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260

ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320 30 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380

ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa 35 cgtcttctca 1440

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct

ctccctgtct 1500

ccgggtaaga attatggtgg cggtggctcg ggcggtggtg gatctggagg aggtgggagt 1560 5 gggaattatg gtggcggtgg ctcgggcggt ggtggatctg gaggaggtgg 2021201261

gagtgggaat 1620

tctcaggtgc agctgaagga gtcaggacct ggcctggtgg cgccctcaca 10 gagcctgtcc 1680

atcacatgca ccgtctcagg gttctcatta accggctatg gtgtaaactg ggttcgccag 1740

15 cctccaggaa agggtctgga gtggctggga atgatatggg gtgatggaag cacagactat 1800

aattcagctc tcaaatccag actatcgatc accaaggaca actccaagag ccaagttttc 1860 20 ttaaaaatga acagtctgca aactgatgac acagccagat actactgtgc tcgagatggt 1920

tatagtaact ttcattacta tgttatggac tactggggtc aaggaacctc 25 agtcaccgtc 1980

tcctctgggg gtggaggctc tggtggcggt ggatccggcg gaggtgggtc gggtggcggc 2040

30 ggatctgaca ttgtgctcac ccaatctcca gcttctttgg ctgtgtctct aggtcagaga 2100

gccaccatct cctgcagagc cagtgaaagt gttgaatatt atgtcacaag tttaatgcag 2160 35 tggtaccaac agaaaccagg acagccaccc aaactcctca tctctgctgc tagcaacgta 2220

gaatctgggg tccctgccag gtttagtggc agtgggtctg ggacagactt tagcctcaac 2280

5 atccatcctg tggaggagga tgatattgca atgtatttct gtcagcaaag taggaaggtt 2340 2021201261

ccatggacgt tcggtggagg caccaagctg gaaatcaaac gttaatctag a 2391 10

<210> 180 <211> 790 <212> PRT 15 <213> Artificial sequence

<220> <223> Synthetic polypeptide

20 <220> <223> 2H7sssIgG1-STD2-2e12HL (w/2e12 leader) (AA)

<220> <221> misc_feature 25 <222> (1)..(22) <223> Leader

<220> <221> misc_feature 30 <222> (23)..(128) <223> VL

<220> <221> misc_feature 35 <222> (129)..(144) <223> Linker

<220> <221> misc_feature <222> (145)..(265) <223> VH 5 <220> 2021201261

<221> misc_feature <222> (268)..(282) <223> Hinge 10 <220> <221> misc_feature <222> (500)..(537) <223> EFD-BD2 Linker 15 <220> <221> misc_feature <222> (538)..(658) <223> VH2 20 <220> <221> misc_feature <222> (659)..(678) <223> Linker2 25 <220> <221> misc_feature <222> (679)..(790) <223> VL2 30 <400> 180 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 35

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro

Ala Ile 20 25 30

5 Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 2021201261

35 40 45

10 Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60

15 Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80

20 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95

25 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105 110

30 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 115 120 125

35 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser

130 135 140

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro 5 Gly Ala 145 150 155 2021201261

160

10 Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175

15 Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190

20 Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205

25 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220

30 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230 235 240 35

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp

Val Trp 245 250 255

5 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 2021201261

260 265 270

10 Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala Pro Glu Leu Leu Gly 275 280 285

15 Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300

20 Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320

25 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335 30

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350 35

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu

Asn Gly 355 360 365

5 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 2021201261

370 375 380

10 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400

15 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 20

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 25

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 30

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 35

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser

Val Met 465 470 475 480

5 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 2021201261

Leu Ser 485 490 495 10

Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 500 505 510 15 Gly Gly Gly Ser Gly Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly 515 520 525

20 Ser Gly Gly Gly Gly Ser Gly Asn Ser Gln Val Gln Leu Lys Glu Ser 530 535 540

25 Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr 545 550 555 560 30

Val Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln 565 570 35 575

Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly 580 585 590

5 Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile 2021201261

Thr Lys 595 600 605

10 Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr 610 615 620

15 Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe 625 630 635 640 20

His Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 645 650 25 655

Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 30 660 665 670

Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser 35 675 680 685

Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser 690 695 700

5 Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr 2021201261

Gln Gln 705 710 715 720 10

Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val 725 730 15 735

Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 20 740 745 750

Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr 25 755 760 765

Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr 770 775 780 30

Lys Leu Glu Ile Lys Arg 785 790

35 <210> 181 <211> 790

<212> PRT <213> Artificial sequence

<220> 5 <223> Synthetic polypeptide 2021201261

<220> <223> 2H7sssIgG1(P238S/P331S)-STD2-2e12HL (w/2e12 leader) (AA) 10 <220> <221> misc_feature <222> (1)..(22) <223> Leader 15 <220> <221> misc_feature <222> (23)..(128) <223> VL 20 <220> <221> misc_feature <222> (129)..(144) <223> Linker 25 <220> <221> misc_feature <222> (145)..(265) <223> VH 30 <220> <221> misc_feature <222> (268)..(282) <223> Hinge 35 <220> <221> misc_feature

<222> (500)..(537) <223> EFD-BD2 Linker

<220> 5 <221> misc_feature <222> (538)..(658) 2021201261

<223> VH2

<220> 10 <221> misc_feature <222> (659)..(678) <223> Linker2

<220> 15 <221> misc_feature <222> (679)..(790) <223> VL2

<400> 181 20 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15

25 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30

30 Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45

35 Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser

50 55 60

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly 5 Val Pro 65 70 75 2021201261

80

10 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95

15 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105 110

20 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 115 120 125

25 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140

30 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160

35 Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr

165 170 175

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu 5 Trp Ile 180 185 190 2021201261

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln 10 Lys Phe 195 200 205

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr 15 Ala Tyr 210 215 220

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 20 Phe Cys 225 230 235 240

25 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 245 250 255

30 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270

35 Asp Lys Thr His Thr Ser Pro Pro Ser Ser Ala Pro Glu Leu Leu Gly

275 280 285

Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 5 Leu Met 290 295 300 2021201261

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 10 Ser His 305 310 315 320

15 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335

20 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350

25 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355 360 365

30 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Ser Ile 370 375 380

35 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val

385 390 395 400

5 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 2021201261

405 410 415

10 Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430

15 Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445

20 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460

25 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475 480

30 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 485 490 495 35

Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly

Ser Gly 500 505 510

5 Gly Gly Gly Ser Gly Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly 2021201261

515 520 525

10 Ser Gly Gly Gly Gly Ser Gly Asn Ser Gln Val Gln Leu Lys Glu Ser 530 535 540

15 Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr 545 550 555 560

20 Val Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln 565 570 575 25

Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly 580 585 590 30

Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys 595 600 605 35

Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu

Gln Thr 610 615 620

5 Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe 2021201261

625 630 635 640

10 His Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 645 650 655 15

Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 660 665 670 20

Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser 675 680 685 25 Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser 690 695 700

30 Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln 705 710 715 720 35

Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser

Asn Val 725 730 735

5 Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly 2021201261

Thr Asp 740 745 750

10 Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr 755 760 765

15 Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr 770 775 780

20 Lys Leu Glu Ile Lys Arg 785 790

25 <210> 182 <211> 2283 <212> DNA <213> Artificial sequence

30 <220> <223> Synthetic polynucleotide

<220> <223> 2H7sssIgG1-H1-2e12HL (DNA) 35 <400> 182 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat

cagtgcttca 60

gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 5 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta 2021201261

catgcactgg 180

taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc 10 caacctggct 240

tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300

15 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360

cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420 20 ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg 480

aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt 25 taccagttac 540

aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600

30 ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660

gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 35 gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac 840

5 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900 2021201261

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960 10 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg 15 tgtggtcagc 1080

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140

20 aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga 1200

gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 25 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga 30 cggctccttc 1380

ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440

35 tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500

ccgggtaaga attctcaggt gcagctgaag gagtcaggac ctggcctggt ggcgccctca 1560

cagagcctgt ccatcacatg caccgtctca gggttctcat taaccggcta 5 tggtgtaaac 1620 2021201261

tgggttcgcc agcctccagg aaagggtctg gagtggctgg gaatgatatg gggtgatgga 1680

10 agcacagact ataattcagc tctcaaatcc agactatcga tcaccaagga caactccaag 1740

agccaagttt tcttaaaaat gaacagtctg caaactgatg acacagccag atactactgt 1800 15 gctcgagatg gttatagtaa ctttcattac tatgttatgg actactgggg tcaaggaacc 1860

tcagtcaccg tctcctctgg gggtggaggc tctggtggcg gtggatccgg 20 cggaggtggg 1920

tcgggtggcg gcggatctga cattgtgctc acccaatctc cagcttcttt ggctgtgtct 1980

25 ctaggtcaga gagccaccat ctcctgcaga gccagtgaaa gtgttgaata ttatgtcaca 2040

agtttaatgc agtggtacca acagaaacca ggacagccac ccaaactcct catctctgct 2100 30 gctagcaacg tagaatctgg ggtccctgcc aggtttagtg gcagtgggtc tgggacagac 2160

tttagcctca acatccatcc tgtggaggag gatgatattg caatgtattt 35 ctgtcagcaa 2220

agtaggaagg ttccatggac gttcggtgga ggcaccaagc tggaaatcaa

acgttaatct 2280

aga 2283 5 2021201261

<210> 183 <211> 754 <212> PRT 10 <213> Artificial sequence

<220> <223> Synthetic polypeptide

15 <220> <223> 2H7sssIgG1-H1-2e12HL (w/2e12 leader) (AA)

<220> <221> misc_feature 20 <222> (1)..(22) <223> Leader

<220> <221> misc_feature 25 <222> (23)..(128) <223> VL

<220> <221> misc_feature 30 <222> (129)..(144) <223> Linker

<220> <221> misc_feature 35 <222> (145)..(265) <223> VH

<220> <221> misc_feature <222> (268)..(281) <223> Hinge 5 <220> 2021201261

<221> misc_feature <222> (500)..(501) <223> EFD-BD2 Linker 10 <220> <221> misc_feature <222> (502)..(622) <223> VH2 15 <220> <221> misc_feature <222> (623)..(642) <223> Linker2 20 <220> <221> misc_feature <222> (643)..(754) <223> VL2 25 <400> 183

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 30 1 5 10 15

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 35 20 25 30

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45

5 Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly 2021201261

Ser Ser 50 55 60

10 Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80 15

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95 20

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105 110 25

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 115 120 125 30

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140 35

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro

Gly Ala 145 150 155 160

5 Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 2021201261

Ser Tyr 165 170 175 10

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 15

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 20

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 25

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230 235 30 240

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 35 245 250

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 5 2021201261

Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285 10

Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 15

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 20 320

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 25 325 330 335

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 30 Thr Tyr 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 35 Asn Gly 355 360 365

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 370 375 380 5 2021201261

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 10 400

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 15 405 410 415

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 20 Val Glu 420 425 430

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 25 Pro Pro 435 440 445

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 30 Thr Val 450 455 460

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 35 Val Met 465 470 475

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 485 490 5 495 2021201261

Pro Gly Lys Asn Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu 10 500 505 510

Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe 15 515 520 525

Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys 20 530 535 540

Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr 25 545 550 555 560

Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 30 Ser Lys 565 570 575

35 Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala 580 585 590

Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val 5 595 600 605 2021201261

Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly 10 610 615 620

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 15 625 630 635 640

Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala 20 Val Ser 645 650 655

25 Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Glu 660 665 670

30 Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln 675 680 685

35 Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val 690 695 700

Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn 5 705 710 715 720 2021201261

Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys 10 Gln Gln 725 730 735

Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 15 Glu Ile 740 745 750

Lys Arg 20

<210> 184 <211> 2301 25 <212> DNA <213> Artificial sequence

<220> <223> Synthetic polynucleotide 30 <220> <223> 2H7sssIgG1-H2-2e12HL (DNA)

<400> 184 35 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60

gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120

ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta 5 catgcactgg 180 2021201261

taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240

10 tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300

agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360 15 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420

ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc 20 tgagtcggtg 480

aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540

25 aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600

ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660 30 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720

gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt 35 cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga

caaaactcac 840

acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900 5 ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg 2021201261

cgtggtggtg 960

gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg 10 cgtggaggtg 1020

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080

15 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140

aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga 1200 20 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260

ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg 25 ggagagcaat 1320 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380

ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa 30 cgtcttctca 1440

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500

35 ccgggtaagg gtggcggtgg ctcggggaat tctcaggtgc agctgaagga gtcaggacct 1560

ggcctggtgg cgccctcaca gagcctgtcc atcacatgca ccgtctcagg gttctcatta 1620

accggctatg gtgtaaactg ggttcgccag cctccaggaa agggtctgga 5 gtggctggga 1680 2021201261

atgatatggg gtgatggaag cacagactat aattcagctc tcaaatccag actatcgatc 1740

10 accaaggaca actccaagag ccaagttttc ttaaaaatga acagtctgca aactgatgac 1800

acagccagat actactgtgc tcgagatggt tatagtaact ttcattacta tgttatggac 1860 15 tactggggtc aaggaacctc agtcaccgtc tcctctgggg gtggaggctc tggtggcggt 1920

ggatccggcg gaggtgggtc gggtggcggc ggatctgaca ttgtgctcac 20 ccaatctcca 1980

gcttctttgg ctgtgtctct aggtcagaga gccaccatct cctgcagagc cagtgaaagt 2040

25 gttgaatatt atgtcacaag tttaatgcag tggtaccaac agaaaccagg acagccaccc 2100

aaactcctca tctctgctgc tagcaacgta gaatctgggg tccctgccag gtttagtggc 2160 30 agtgggtctg ggacagactt tagcctcaac atccatcctg tggaggagga tgatattgca 2220

atgtatttct gtcagcaaag taggaaggtt ccatggacgt tcggtggagg 35 caccaagctg 2280

gaaatcaaac gttaatctag a

<210> 185 5 <211> 760 <212> PRT 2021201261

<213> Artificial sequence

<220> 10 <223> Synthetic polypeptide

<220> <223> 2H7sssIgG1-H2-2e12HL (w/2e12 leader) (AA)

15 <220> <221> misc_feature <222> (1)..(22) <223> Leader

20 <220> <221> misc_feature <222> (23)..(127) <223> VL

25 <220> <221> misc_feature <222> (128)..(144) <223> Linker

30 <220> <221> misc_feature <222> (145)..(265) <223> VH

35 <220> <221> misc_feature <222> (268)..(282)

<223> Hinge

<220> <221> misc_feature 5 <222> (500)..(507) <223> EFD-BD2 Linker 2021201261

<220> <221> misc_feature 10 <222> (508)..(628) <223> VH2

<220> <221> misc_feature 15 <222> (629)..(648) <223> Linker2

<220> <221> misc_feature 20 <222> (649)..(760) <223> VL2

<400> 185

25 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15

30 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30

35 Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 5 50 55 60 2021201261

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 10 80

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 15 85 90 95

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 20 100 105 110

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 25 115 120 125

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 30 130 135 140

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 35 145 150 155

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 5 175 2021201261

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 10 180 185 190

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 15 195 200 205

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 20 210 215 220

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 25 225 230 235 240

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp 30 Val Trp 245 250 255

35 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270

Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly 5 275 280 285 2021201261

Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 10 290 295 300

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 15 305 310 315 320

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 20 325 330 335

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 25 Thr Tyr 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 30 Asn Gly 355 360 365

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 35 Pro Ile 370 375 380

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 5 400 2021201261

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 10 405 410 415

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 15 Val Glu 420 425 430

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 20 Pro Pro 435 440 445

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 25 Thr Val 450 455 460

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 30 Val Met 465 470 475 480

35 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 485 490

Pro Gly Lys Gly Gly Gly Gly Ser Gly Asn Ser Gln Val Gln 5 Leu Lys 500 505 510 2021201261

Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser 10 Ile Thr 515 520 525

Cys Thr Val Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn 15 Trp Val 530 535 540

Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile 20 Trp Gly 545 550 555 560

25 Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile 565 570 575

30 Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu 580 585 590

35 Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser 595 600 605

Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 5 610 615 620 2021201261

Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 10 625 630 635 640

Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln 15 Ser Pro 645 650 655

20 Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg 660 665 670

25 Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr 675 680 685

30 Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser 690 695 700

35 Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly 705 710 715

Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp 5 Ile Ala 725 730 2021201261

735

10 Met Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly 740 745 750

15 Gly Thr Lys Leu Glu Ile Lys Arg 755 760

<210> 186 20 <211> 2307 <212> DNA <213> Artificial sequence

<220> 25 <223> Synthetic polynucleotide

<220> <223> 2H7sssIgG1-H3-2e12HL (DNA)

30 <400> 186 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60

gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct 35 gtctgcatct 120 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180

taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240

5 tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300 2021201261

agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360 10 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420

ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc 15 tgagtcggtg 480

aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540

20 aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600

ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660 25 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720

gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt 30 cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac 840

35 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960

gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg 5 cgtggaggtg 1020 2021201261

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080

10 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140

aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga 1200 15 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260

ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg 20 ggagagcaat 1320

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380

25 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500 30 ccgggtaaga attatggtgg cggtggctct gggaattctc aggtgcagct gaaggagtca 1560

ggacctggcc tggtggcgcc ctcacagagc ctgtccatca catgcaccgt 35 ctcagggttc 1620

tcattaaccg gctatggtgt aaactgggtt cgccagcctc caggaaaggg

tctggagtgg 1680

ctgggaatga tatggggtga tggaagcaca gactataatt cagctctcaa atccagacta 1740 5 tcgatcacca aggacaactc caagagccaa gttttcttaa aaatgaacag 2021201261

tctgcaaact 1800

gatgacacag ccagatacta ctgtgctcga gatggttata gtaactttca 10 ttactatgtt 1860

atggactact ggggtcaagg aacctcagtc accgtctcct ctgggggtgg aggctctggt 1920

15 ggcggtggat ccggcggagg tgggtcgggt ggcggcggat ctgacattgt gctcacccaa 1980

tctccagctt ctttggctgt gtctctaggt cagagagcca ccatctcctg cagagccagt 2040 20 gaaagtgttg aatattatgt cacaagttta atgcagtggt accaacagaa accaggacag 2100

ccacccaaac tcctcatctc tgctgctagc aacgtagaat ctggggtccc tgccaggttt 2160 25 agtggcagtg ggtctgggac agactttagc ctcaacatcc atcctgtgga ggaggatgat 2220

attgcaatgt atttctgtca gcaaagtagg aaggttccat ggacgttcgg 30 tggaggcacc 2280

aagctggaaa tcaaacgtta atctaga 2307

35 <210> 187 <211> 762

<212> PRT <213> Artificial sequence

<220> 5 <223> Synthetic polypeptide 2021201261

<220> <223> 2H7sssIgG1-H3-2e12HL (w/2e12 leader) (AA)

10 <220> <221> misc_feature <222> (1)..(22) <223> Leader

15 <220> <221> misc_feature <222> (23)..(128) <223> VL <220> 20 <221> misc_feature <222> (129)..(144) <223> Linker

<220> 25 <221> misc_feature <222> (145)..(265) <223> VH

<220> 30 <221> misc_feature <222> (268)..(282) <223> Hinge

<220> 35 <221> misc_feature <222> (500)..(509) <223> EFD-BD2 Linker

<220> <221> misc_feature <222> (510)..(630) 5 <223> VH2 2021201261

<220> <221> misc_feature <222> (631)..(650) 10 <223> Linker2

<220> <221> misc_feature <222> (651)..(762) 15 <223> VL2

<400> 187

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser 20 Ala Ser 1 5 10 15

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro 25 Ala Ile 20 25 30

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg 30 Ala Ser 35 40 45

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly 35 Ser Ser 50 55 60

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 5 80 2021201261

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 10 85 90 95

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 15 100 105 110

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 20 115 120 125

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 25 130 135 140

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 30 145 150 155 160

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 35 165 170

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190 5 2021201261

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205 10

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 15

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230 235 20 240

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 25 245 250 255

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys 30 Ser Ser 260 265 270

Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu 35 Leu Gly 275 280 285

Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 5 2021201261

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 10 320

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 15 325 330 335

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 20 Thr Tyr 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 25 Asn Gly 355 360 365

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 30 Pro Ile 370 375 380

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 35 Gln Val 385 390 395

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 5 405 410 415 2021201261

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 10 420 425 430

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 15 435 440 445

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 20 450 455 460

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 25 465 470 475 480

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 30 Leu Ser 485 490 495

35 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Asn Ser Gln Val Gln 500 505 510

Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser 5 515 520 525 2021201261

Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn 10 530 535 540

Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile 15 545 550 555 560

Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser 20 Arg Leu 565 570 575

25 Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn 580 585 590

30 Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly 595 600 605

35 Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr 610 615 620

Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 5 625 630 635 640 2021201261

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu 10 Thr Gln 645 650 655

15 Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser 660 665 670

Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu 20 Met Gln 675 680 685

Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 25 Ser Ala 690 695 700

Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly 30 Ser Gly 705 710 715 720

35 Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp 725 730

Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp 5 Thr Phe 740 745 750 2021201261

Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 10 755 760

<210> 188 <211> 2316 15 <212> DNA <213> Artificial sequence

<220> <223> Synthetic polynucleotide 20 <220> <223> 2H7sssIgG1-H4-2e12HL (DNA)

<400> 188 25 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60

gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 30 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180

taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc 35 caacctggct 240

tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc

tctcacaatc 300

agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360 5 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc 2021201261

gggcggtggt 420

ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc 10 tgagtcggtg 480

aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540

15 aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600

ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660 20 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720

gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt 25 cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac 840 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt 30 cctcttcccc 900

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960

35 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg 5 caaggtctcc 1140 2021201261

aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga 1200

10 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260

ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320 15 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380

ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa 20 cgtcttctca 1440

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500

25 ccgggtaagg gtggcggtgg ctcgggcggt ggtggatctg ggaattctca ggtgcagctg 1560

aaggagtcag gacctggcct ggtggcgccc tcacagagcc tgtccatcac atgcaccgtc 1620 30 tcagggttct cattaaccgg ctatggtgta aactgggttc gccagcctcc aggaaagggt 1680

ctggagtggc tgggaatgat atggggtgat ggaagcacag actataattc 35 agctctcaaa 1740

tccagactat cgatcaccaa ggacaactcc aagagccaag ttttcttaaa

aatgaacagt 1800

ctgcaaactg atgacacagc cagatactac tgtgctcgag atggttatag taactttcat 1860 5 tactatgtta tggactactg gggtcaagga acctcagtca ccgtctcctc 2021201261

tgggggtgga 1920

ggctctggtg gcggtggatc cggcggaggt gggtcgggtg gcggcggatc 10 tgacattgtg 1980

ctcacccaat ctccagcttc tttggctgtg tctctaggtc agagagccac catctcctgc 2040

15 agagccagtg aaagtgttga atattatgtc acaagtttaa tgcagtggta ccaacagaaa 2100

ccaggacagc cacccaaact cctcatctct gctgctagca acgtagaatc tggggtccct 2160 20 gccaggttta gtggcagtgg gtctgggaca gactttagcc tcaacatcca tcctgtggag 2220

gaggatgata ttgcaatgta tttctgtcag caaagtagga aggttccatg 25 gacgttcggt 2280

ggaggcacca agctggaaat caaacgttaa tctaga 2316

30 <210> 189 <211> 765 <212> PRT <213> Artificial sequence 35 <220> <223> Synthetic polypeptide

<220> <223> 2H7sssIgG1-H4-2e12HL (AA)

5 <220> <221> misc_feature 2021201261

<222> (1)..(22) <223> Leader

10 <220> <221> misc_feature <222> (23)..(128) <223> VL

15 <220> <221> misc_feature <222> (129)..(144) <223> Linker

20 <220> <221> misc_feature <222> (145)..(265) <223> VH

25 <220> <221> misc_feature <222> (268)..(282) <223> Hinge

30 <220> <221> misc_feature <222> (500)..(512) <223> EFD-BD2 Linker

35 <220> <221> misc_feature <222> (513)..(633)

<223> VH2

<220> <221> misc_feature 5 <222> (634)..(653) <223> Linker2 2021201261

<220> <221> misc_feature 10 <222> (654)..(765) <223> VL2

<400> 189 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser 15 Ala Ser 1 5 10 15

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro 20 Ala Ile 20 25 30

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg 25 Ala Ser 35 40 45

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly 30 Ser Ser 50 55 60

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly 35 Val Pro 65 70 75

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 5 85 90 95 2021201261

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 10 100 105 110

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 15 115 120 125

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 20 130 135 140

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 25 145 150 155 160

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 30 Ser Tyr 165 170 175

35 Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 5 195 200 205 2021201261

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 10 210 215 220

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 15 225 230 235 240

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp 20 Val Trp 245 250 255

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys 25 Ser Ser 260 265 270

Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu 30 Leu Gly 275 280 285

Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 35 Leu Met 290 295 300

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 5 320 2021201261

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 10 325 330 335

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 15 Thr Tyr 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 20 Asn Gly 355 360 365

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 25 Pro Ile 370 375 380

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 30 Gln Val 385 390 395 400

35 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 5 Val Glu 420 425 430 2021201261

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 10 Pro Pro 435 440 445

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 15 Thr Val 450 455 460

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 20 Val Met 465 470 475 480

25 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 485 490 495

30 Pro Gly Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Asn Ser 500 505 510

35 Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 515 520 525

Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Gly Tyr 5 530 535 540 2021201261

Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 10 545 550 555 560

Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala 15 Leu Lys 565 570 575

20 Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 580 585 590

25 Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala 595 600 605

30 Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly 610 615 620

35 Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 625 630 635

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 5 Ile Val 645 650 2021201261

655

10 Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala 660 665 670

15 Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser 675 680 685

20 Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu 690 695 700

25 Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser 705 710 715 720

30 Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu 725 730 735 35

Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys

Val Pro 740 745 750

5 Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 755 760 765 2021201261

<210> 190 <211> 2322 10 <212> DNA <213> Artificial sequence

<220> <223> Synthetic polynucleotide 15 <220> <223> 2H7sssIgG1-H5-2e12HL (DNA)

<400> 190 20 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60

gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 25 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180

taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc 30 caacctggct 240

tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300

35 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360

cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420

ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc 5 tgagtcggtg 480 2021201261

aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540

10 aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600

ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660 15 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720

gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt 20 cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac 840

25 acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960 30 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg 35 tgtggtcagc 1080

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg

caaggtctcc 1140

aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg gcagccccga 1200 5 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa 2021201261

ccaggtcagc 1260

ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg 10 ggagagcaat 1320

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380

15 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500 20 ccgggtaaga attatggtgg cggtggctcg ggcggtggtg gatctgggaa ttctcaggtg 1560 cagctgaagg agtcaggacc tggcctggtg gcgccctcac agagcctgtc catcacatgc 1620 25 accgtctcag ggttctcatt aaccggctat ggtgtaaact gggttcgcca gcctccagga 1680

aagggtctgg agtggctggg aatgatatgg ggtgatggaa gcacagacta 30 taattcagct 1740

ctcaaatcca gactatcgat caccaaggac aactccaaga gccaagtttt cttaaaaatg 1800

35 aacagtctgc aaactgatga cacagccaga tactactgtg ctcgagatgg ttatagtaac 1860

tttcattact atgttatgga ctactggggt caaggaacct cagtcaccgt ctcctctggg 1920

ggtggaggct ctggtggcgg tggatccggc ggaggtgggt cgggtggcgg 5 cggatctgac 1980 2021201261

attgtgctca cccaatctcc agcttctttg gctgtgtctc taggtcagag agccaccatc 2040

10 tcctgcagag ccagtgaaag tgttgaatat tatgtcacaa gtttaatgca gtggtaccaa 2100

cagaaaccag gacagccacc caaactcctc atctctgctg ctagcaacgt agaatctggg 2160 15 gtccctgcca ggtttagtgg cagtgggtct gggacagact ttagcctcaa catccatcct 2220

gtggaggagg atgatattgc aatgtatttc tgtcagcaaa gtaggaaggt 20 tccatggacg 2280

ttcggtggag gcaccaagct ggaaatcaaa cgttaatcta ga 2322

25 <210> 191 <211> 767 <212> PRT <213> Artificial sequence 30 <220> <223> Synthetic polypeptide

<220> 35 <223> 2H7sssIgG1-H5-2e12HL (w/2e12 leader) (AA)

<220>

<221> misc_feature <222> (1)..(22) <223> Leader

5 <220> <221> misc_feature 2021201261

<222> (23)..(128) <223> VL

10 <220> <221> misc_feature <222> (129)..(144) <223> Linker

15 <220> <221> misc_feature <222> (145)..(265) <223> VH

20 <220> <221> misc_feature <222> (268)..(282) <223> Hinge

25 <220> <221> misc_feature <222> (500)..(514) <223> EFD-BD2 Linker

30 <220> <221> misc_feature <222> (515)..(635) <223> VH2

35 <220> <221> misc_feature <222> (636)..(655)

<223> Linker2

<220> <221> misc_feature 5 <222> (656)..(767) <223> VL2 2021201261

<400> 191

10 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15

15 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30

20 Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45

25 Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60

30 Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80

35 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile

85 90 95

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 5 100 105 110 2021201261

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 10 115 120 125

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 15 130 135 140

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 20 145 150 155 160

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 25 Ser Tyr 165 170 175

30 Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190

35 Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 5 210 215 220 2021201261

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 10 225 230 235 240

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp 15 Val Trp 245 250 255

20 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270

25 Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly 275 280 285

30 Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300

35 Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 5 Glu Val 325 330 2021201261

335

10 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 15 Asn Gly 355 360 365

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 20 Pro Ile 370 375 380

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 25 Gln Val 385 390 395 400

30 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415

35 Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu

420 425 430

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 5 Pro Pro 435 440 445 2021201261

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 10 Thr Val 450 455 460

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 15 Val Met 465 470 475 480

20 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 485 490 495

25 Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 500 505 510

30 Asn Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro 515 520 525

35 Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr

530 535 540

Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys Gly 5 Leu Glu 545 550 555 2021201261

560

10 Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala 565 570 575

15 Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val 580 585 590

20 Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr 595 600 605

25 Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr 610 615 620

30 Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser 625 630 635 640

35 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp

645 650 655

5 Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln 2021201261

660 665 670

10 Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val 675 680 685

15 Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys 690 695 700

20 Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg 705 710 715 720

25 Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro 725 730 735 30

Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys 740 745 750 35

Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys

Arg 755 760 765

5 <210> 192 <211> 2331 2021201261

<212> DNA <213> Artificial sequence

10 <220> <223> Synthetic polynucleotide

<220> <223> 2H7sssIgG1-H6-2e12HL (DNA) 15 <400> 192 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60

20 gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120

ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 25 taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240

tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc 30 tctcacaatc 300

agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360

35 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420

ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg 480

aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt 5 taccagttac 540 2021201261

aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600

10 ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660

gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 15 gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga 20 caaaactcac 840

acatccccac cgagcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900

25 ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960

gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020 30 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg 35 caaggtctcc 1140

aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg

gcagccccga 1200

gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 5 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg 2021201261

ggagagcaat 1320

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga 10 cggctccttc 1380

ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440

15 tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500

ccgggtaagg gtggcggtgg ctcgggcggt ggtggatctg ggggaggagg cagcgggaat 1560 20 tctcaggtgc agctgaagga gtcaggacct ggcctggtgg cgccctcaca gagcctgtcc 1620

atcacatgca ccgtctcagg gttctcatta accggctatg gtgtaaactg 25 ggttcgccag 1680

cctccaggaa agggtctgga gtggctggga atgatatggg gtgatggaag cacagactat 1740

30 aattcagctc tcaaatccag actatcgatc accaaggaca actccaagag ccaagttttc 1800

ttaaaaatga acagtctgca aactgatgac acagccagat actactgtgc tcgagatggt 1860 35 tatagtaact ttcattacta tgttatggac tactggggtc aaggaacctc agtcaccgtc 1920

tcctctgggg gtggaggctc tggtggcggt ggatccggcg gaggtgggtc gggtggcggc 1980

5 ggatctgaca ttgtgctcac ccaatctcca gcttctttgg ctgtgtctct aggtcagaga 2040 2021201261

gccaccatct cctgcagagc cagtgaaagt gttgaatatt atgtcacaag tttaatgcag 2100 10 tggtaccaac agaaaccagg acagccaccc aaactcctca tctctgctgc tagcaacgta 2160

gaatctgggg tccctgccag gtttagtggc agtgggtctg ggacagactt 15 tagcctcaac 2220

atccatcctg tggaggagga tgatattgca atgtatttct gtcagcaaag taggaaggtt 2280

20 ccatggacgt tcggtggagg caccaagctg gaaatcaaac gttaatctag a 2331

<210> 193 25 <211> 770 <212> PRT <213> Artificial sequence

<220> 30 <223> Synthetic polypeptide

<220> <223> 2H7sssIgG1-H6-2e12HL (w/2e12 leader) (AA)

35 <220> <221> misc_feature <222> (1)..(22)

<223> Leader

<220> <221> misc_feature 5 <222> (23)..(128) <223> VL 2021201261

<220> <221> misc_feature 10 <222> (129)..(144) <223> Linker

<220> <221> misc_feature 15 <222> (145)..(265) <223> VH

<220> <221> misc_feature 20 <222> (268)..(282) <223> Hinge

<220> <221> misc_feature 25 <222> (500)..(517) <223> EFD-BD2 Linker

<220> <221> misc_feature 30 <222> (518)..(638) <223> VH2

<220> <221> misc_feature 35 <222> (639)..(658) <223> Linker2

<220> <221> misc_feature <222> (659)..(770) <223> VL2 5 <400> 193 2021201261

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 10 1 5 10 15

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 15 20 25 30

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 20 35 40 45

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 25 50 55 60

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 30 65 70 75 80

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu 35 Thr Ile 85 90 95

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105 110 5 2021201261

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 115 120 125 10

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140 15

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 20 160

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 25 165 170 175

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu 30 Trp Ile 180 185 190

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 35 195 200 205

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220

5 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 2021201261

Phe Cys 225 230 235 240 10

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 245 250 15 255

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 20 260 265 270

Asp Lys Thr His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly 25 275 280 285

Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 30 290 295 300

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 35 305 310 315

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 5 335 2021201261

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 10 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 15 355 360 365

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 20 370 375 380

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 25 385 390 395 400

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 30 Val Ser 405 410 415

35 Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 5 435 440 445 2021201261

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 10

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475 15 480

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 20 485 490 495

Pro Gly Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 25 Gly Gly 500 505 510

Gly Ser Gly Asn Ser Gln Val Gln Leu Lys Glu Ser Gly Pro 30 Gly Leu 515 520 525

Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser 35 Gly Phe 530 535 540

Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys 545 550 555 5 560 2021201261

Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr 10 565 570 575

Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 15 Ser Lys 580 585 590

Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 20 Thr Ala 595 600 605

Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr 25 Tyr Val 610 615 620

Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 30 Gly Gly 625 630 635 640

35 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 645 650

Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala 5 Val Ser 660 665 670 2021201261

Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser 10 Val Glu 675 680 685

Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 15 Gly Gln 690 695 700

Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val 20 705 710 715 720

Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 25 Leu Asn 725 730 735

30 Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln 740 745 750

35 Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 755 760 765

Lys Arg 770 5 2021201261

<210> 194 <211> 2301 <212> DNA 10 <213> Artificial sequence

<220> <223> Synthetic polynucleotide

15 <220> <223> 2H7sscIgG1-H7-2e12HL (DNA)

<400> 194 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat 20 cagtgcttca 60

gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120

25 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180

taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240 30 tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300

agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag 35 ttttaaccca 360

cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc

gggcggtggt 420

ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg 480 5 aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt 2021201261

taccagttac 540

aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg 10 agctatttat 600

ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660

15 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720

gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780 20 ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac 840

acatccccac cgtgcccagc acctgaactc ctggggggat cgtcagtctt 25 cctcttcccc 900

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960

30 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080 35 gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140

aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga 1200

5 gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 2021201261

ctgacctgcc tggtcaaagg cttctatcca agcgacatcg ccgtggagtg ggagagcaat 1320 10 gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380

ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa 15 cgtcttctca 1440

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500

20 ccgggtaagg ggtgtccacc ttgtccgaat tctcaggtgc agctgaagga gtcagggcct 1560

ggcagcgtgg cgccctcaca gagcctgtcc atcacatgca ccgtctcagg gttctcatta 1620 25 accggctatg gtgtaaactg ggttcgccag cctccaggaa agggtctgga gtggctggga 1680

atgatatggg gtgatggaag cacagactat aattcagctc tcaaatccag 30 actatcgatc 1740

accaaggaca actccaagag ccaagttttc ttaaaaatga acagtctgca aactgatgac 1800

35 acagccagat actactgtgc tcgagatggt tatagtaact ttcattacta tgttatggac 1860

tactggggtc aaggaacctc agtcaccgtc tcctctgggg gtggaggctc tggtggcggt 1920

ggatccggcg gaggtgggtc gggtggcggc ggatctgaca ttgtgctcac 5 ccaatctcca 1980 2021201261

gcttctttgg ctgtgtctct aggtcagaga gccaccatct cctgcagagc cagtgaaagt 2040

10 gttgaatatt atgtcacaag tttaatgcag tggtaccaac agaaaccagg acagccaccc 2100

aagctcctca tctctgctgc tagcaacgta gaatctgggg tccctgccag gtttagtggc 2160 15 agtgggtctg ggacagactt tagcctcaac atccatcctg tggaggagga tgatattgca 2220

atgtatttct gtcagcaaag taggaaggtt ccatggacgt tcggtggagg 20 caccaagctg 2280

gaaatcaaac gttaatctag a 2301

25 <210> 195 <211> 760 <212> PRT <213> Artificial sequence 30 <220> <223> Synthetic polypeptide

<220> 35 <223> 2H7sscIgG1-H7-2e12HL (w/2e12 linker ) (AA)

<220>

<221> misc_feature <222> (1)..(22) <223> Leader

5 <220> <221> misc_feature 2021201261

<222> (23)..(128) <223> VL

10 <220> <221> misc_feature <222> (129)..(144) <223> Linker

15 <220> <221> misc_feature <222> (145)..(265) <223> VH

20 <220> <221> misc_feature <222> (268)..(282) <223> Hinge

25 <220> <221> misc_feature <222> (500)..(507) <223> EFD-BD2 Linker

30 <220> <221> misc_feature <222> (508)..(628) <223> VH2

35 <220> <221> misc_feature <222> (629)..(648)

<223> Linker2

<220> <221> misc_feature 5 <222> (649)..(760) <223> VL2 2021201261

<400> 195

10 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15

15 Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30

20 Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45

25 Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60

30 Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 80

35 Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile

85 90 95

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 5 Gln Trp 100 105 110 2021201261

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu 10 Leu Lys 115 120 125

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 15 Ser Ser 130 135 140

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro 20 Gly Ala 145 150 155 160

25 Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 175

30 Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 180 185 190

35 Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe

195 200 205

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr 5 Ala Tyr 210 215 220 2021201261

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 10 Phe Cys 225 230 235 240

15 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 245 250 255

20 Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270

25 Asp Lys Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 275 280 285

30 Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300

35 Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 5 Glu Val 325 330 2021201261

335

10 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350

15 Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355 360 365

20 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 370 375 380

25 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 400

30 Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 405 410 415 35

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala

Val Glu 420 425 430

5 Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 2021201261

435 440 445

10 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460

15 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475 480

20 His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 485 490 495 25

Pro Gly Lys Gly Cys Pro Pro Cys Pro Asn Ser Gln Val Gln Leu Lys 500 505 510 30

Glu Ser Gly Pro Gly Ser Val Ala Pro Ser Gln Ser Leu Ser Ile Thr 515 520 525 35

Cys Thr Val Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn

Trp Val 530 535 540

Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile 5 Trp Gly 545 550 555 2021201261

560

10 Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile 565 570 575

15 Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu 580 585 590

20 Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser 595 600 605

25 Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 610 615 620

30 Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 625 630 635 640 35

Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln

Ser Pro 645 650 655

5 Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser 2021201261

Cys Arg 660 665 670

10 Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr 675 680 685

15 Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser 690 695 700

20 Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly 705 710 715 720 25

Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala 725 730 30 735

Met Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly 35 740 745 750

Gly Thr Lys Leu Glu Ile Lys Arg 755 760

5 <210> 196 <211> 2283 2021201261

<212> DNA <213> Artificial sequence

10 <220> <223> Synthetic polynucleotide

<220> <223> 2H7sssIgG1-H7-G194 HL (DNA) 15 <400> 196 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60

20 gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120

ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180 25 taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240

tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc 30 tctcacaatc 300

agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360

35 cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420

ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg 480

aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt 5 taccagttac 540 2021201261

aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600

10 ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660

gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720 15 gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga 20 caaaactcac 840

acatccccac cgtgcccagc acctgaactc ctggggggat cgtcagtctt cctcttcccc 900

25 ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960

gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020 30 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg 35 caaggtctcc 1140

aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg

gcagccccga 1200

gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260 5 ctgacctgcc tggtcaaagg cttctatcca agcgacatcg ccgtggagtg 2021201261

ggagagcaat 1320

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga 10 cggctccttc 1380

ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440

15 tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500

ccgggtaagg ggtgtccacc ttgtccgaat tctgaggtcc agctgcaaca gtctggacct 1560 20 gaactggtga agcctggagc ttcaatgaag atttcctgca aggcctctgg ttactcattc 1620

actggctaca tcgtgaactg gctgaagcag agccatggaa agaaccttga 25 gtggattgga 1680

cttattaatc catacaaagg tcttactacc tacaaccaga aattcaaggg caaggccaca 1740

30 ttaactgtag acaagtcatc cagcacagcc tacatggagc tcctcagtct gacatctgaa 1800

gactctgcag tctattactg tgcaagatct gggtactatg gtgactcgga ctggtacttc 1860 35 gatgtctggg gcgcagggac cacggtcacc gtctcctctg gtggcggtgg ctcgggcggt 1920

ggtggatctg gaggaggtgg gagcgctagc gacatccaga tgacacagac tacatcctcc 1980

5 ctgtctgcct ctctgggaga cagagtcacc atcagttgca gggcaagtca ggacattcgc 2040 2021201261

aattatttaa actggtatca gcagaaacca gatggaactg ttaaactcct gatctactac 2100 10 acatcaagat tacactcagg agtcccatca aggttcagtg gcagtgggtc tggaacagat 2160

tattctctca ccattgccaa cctgcaacca gaagatattg ccacttactt 15 ttgccaacag 2220

ggtaatacgc ttccgtggac gttcggtgga ggcaccaaac tggtaaccaa acggtaatct 2280

20 aga 2283

<210> 197 25 <211> 754 <212> PRT <213> Artificial sequence

<220> 30 <223> Synthetic polypeptide

<220> <223> 2H7sssIgG1-H7-G194 HL (w/2e12 leader) (AA)

35 <220> <221> misc_feature <222> (1)..(22)

<223> Leader

<220> <221> misc_feature 5 <222> (23)..(128) <223> VL 2021201261

<220> <221> misc_feature 10 <222> (129)..(144) <223> Linker

<220> <221> misc_feature 15 <222> (145)..(265) <223> VH1

<220> <221> misc_feature 20 <222> (268)..(282) <223> Hinge

<220> <221> misc_feature 25 <222> (500)..(507) <223> EFD-BD2 Linker

<220> <221> misc_feature 30 <222> (508)..(629) <223> VH2

<220> <221> misc_feature 35 <222> (630)..(646) <223> Linker2

<220> <221> misc_feature <222> (647)..(754) <223> VL2 5 <400> 197 2021201261

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 10 1 5 10 15

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 15 20 25 30

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 20 35 40 45

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 25 50 55 60

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 30 65 70 75 80

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu 35 Thr Ile 85 90 95

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105 110 5 2021201261

Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 115 120 125 10

Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140 15

Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 20 160

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 25 165 170 175

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu 30 Trp Ile 180 185 190

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln 35 Lys Phe 195 200 205

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220 5 2021201261

Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230 235 10 240

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 15 245 250 255

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys 20 Ser Ser 260 265 270

Asp Lys Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu 25 Leu Gly 275 280 285

Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 30 Leu Met 290 295 300

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 35 Ser His 305 310 315

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 5 325 330 335 2021201261

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 10 Thr Tyr 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 15 Asn Gly 355 360 365

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 20 Pro Ile 370 375 380

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 25 Gln Val 385 390 395 400

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 30 Val Ser 405 410 415

35 Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 5 435 440 445 2021201261

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 10 450 455 460

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 15 465 470 475 480

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 20 Leu Ser 485 490 495

25 Pro Gly Lys Gly Cys Pro Pro Cys Pro Asn Ser Glu Val Gln Leu Gln 500 505 510

30 Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Met Lys Ile Ser 515 520 525

35 Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Ile Val Asn Trp Leu 530 535 540

Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile Gly Leu Ile Asn Pro 5 545 550 555 560 2021201261

Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys Phe Lys Gly Lys 10 Ala Thr 565 570 575

15 Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Leu Ser 580 585 590

20 Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ser Gly Tyr 595 600 605

25 Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr 610 615 620

30 Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 625 630 635 640

35 Gly Gly Gly Ser Ala Ser Asp Ile Gln Met Thr Gln Thr Thr Ser Ser

645 650 655

Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg 5 Ala Ser 660 665 670 2021201261

Gln Asp Ile Arg Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro 10 Asp Gly 675 680 685

Thr Val Lys Leu Leu Ile Tyr Tyr Thr Ser Arg Leu His Ser 15 Gly Val 690 695 700

Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser 20 Leu Thr 705 710 715 720

25 Ile Ala Asn Leu Gln Pro Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln 725 730 735

30 Gly Asn Thr Leu Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Val Thr 740 745 750

35 Lys Arg

<210> 198 <211> 2298 5 <212> DNA <213> Artificial sequence 2021201261

<220> <223> Synthetic polynucleotide 10 <220> <223> 2H7sssIgG1-H7-G281 HL (DNA)

<400> 198 15 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60

gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct gtctgcatct 120 20 ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180

taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc 25 caacctggct 240

tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300

30 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360

cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420 35 ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg 480

aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540

5 aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600 2021201261

ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac actgactgta 660 10 gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720

gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt 15 cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttctga caaaactcac 840

20 acatccccac cgtgcccagc acctgaactc ctggggggat cgtcagtctt cctcttcccc 900

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg 960 25 gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg 30 tgtggtcagc 1080

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140

35 aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga 1200

gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260

ctgacctgcc tggtcaaagg cttctatcca agcgacatcg ccgtggagtg 5 ggagagcaat 1320 2021201261

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380

10 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct ctccctgtct 1500 15 ccgggtaagg ggtgtccacc ttgtccgaat tctgaggtcc agctgcaaca gtctggacct 1560

gaactggtga agcctggagc ttcaatgaag atttcctgca aggcctctgg 20 ttactcattc 1620

actggctaca tcgtgaactg gctgaagcag agccatggaa agaaccttga gtggattgga 1680

25 cttattaatc catacaaagg tcttactacc tacaaccaga aattcaaggg caaggccaca 1740

ttaactgtag acaagtcatc cagcacagcc tacatggagc tcctcagtct gacatctgaa 1800 30 gactctgcag tctattactg tgcaagatct gggtactatg gtgactcgga ctggtacttc 1860

gatgtctggg gcgcagggac cacggtcacc gtctcctctg gtggcggtgg 35 ctcgggcggt 1920

ggtggatctg gaggaggtgg gagcggggga ggtggcagtg ctagcgacat

ccagatgaca 1980

cagactacat cctccctgtc tgcctctctg ggagacagag tcaccatcag ttgcagggca 2040 5 agtcaggaca ttcgcaatta tttaaactgg tatcagcaga aaccagatgg 2021201261

aactgttaaa 2100

ctcctgatct actacacatc aagattacac tcaggagtcc catcaaggtt 10 cagtggcagt 2160

gggtctggaa cagattattc tctcaccatt gccaacctgc aaccagaaga tattgccact 2220

15 tacttttgcc aacagggtaa tacgcttccg tggacgttcg gtggaggcac caaactggta 2280

accaaacggt aatctaga 2298 20

<210> 199 <211> 759 <212> PRT 25 <213> Artificial sequence

<220> <223> Synthetic polypeptide

30 <220> <223> 2H7sssIgG1-H7-G281 HL (w/2e12 leader) (AA)

<220> 35 <221> misc_feature <222> (1)..(22) <223> Leader

<220> <221> misc_feature <222> (23)..(128) 5 <223> VL1 2021201261

<220> <221> misc_feature <222> (129)..(144) 10 <223> Linker

<220> <221> misc_feature <222> (145)..(265) 15 <223> VH1

<220> <221> misc_feature <222> (268)..(282) 20 <223> Hinge

<220> <221> misc_feature <222> (500)..(507) 25 <223> EFD-BD2 Linker

<220> <221> misc_feature <222> (508)..(629) 30 <223> VH2

<220> <221> misc_feature <222> (630)..(651) 35 <223> Linker2

<220>

<221> misc_feature <222> (652)..(759) <223> VL2

5 <400> 199 2021201261

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 10

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 15

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 20

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 25

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 30 80

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 35 85 90 95

Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 100 105 110

5 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu 2021201261

Leu Lys 115 120 125

10 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140

15 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160 20

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 25 175

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 30 180 185 190

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 35 195 200 205

Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220

5 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 2021201261

Phe Cys 225 230 235 240 10

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 245 250 15 255

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Ser 260 265 270 20

Asp Lys Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 275 280 285 25

Gly Ser Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 290 295 300 30

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 35 320

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 335 5 2021201261

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 340 345 350 10

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355 360 365 15

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 370 375 380 20

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 25 400

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 30 405 410 415

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 35 Val Glu 420 425 430

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 5 2021201261

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 10

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475 15 480

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 20 485 490 495

Pro Gly Lys Gly Cys Pro Pro Cys Pro Asn Ser Glu Val Gln 25 Leu Gln 500 505 510

Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Met Lys Ile Ser 30 515 520 525

Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Ile Val Asn Trp Leu 35 530 535 540

Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile Gly Leu Ile Asn Pro 545 550 555 560 5 2021201261

Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys Phe Lys Gly Lys Ala Thr 565 570 10 575

Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Leu Ser 15 580 585 590

Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ser Gly Tyr 20 595 600 605

Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr 25 610 615 620

Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 30 625 630 635 640

Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Asp Ile Gln 35 Met Thr 645 650

Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile 5 660 665 670 2021201261

Ser Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr Leu Asn Trp Tyr Gln 10 675 680 685

Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr Tyr Thr Ser Arg 15 690 695 700

Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 20 705 710 715 720

Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro Glu Asp Ile 25 Ala Thr 725 730 735

30 Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Trp Thr Phe Gly Gly Gly 740 745 750

35 Thr Lys Leu Val Thr Lys Arg

<210> 200 <211> 1533 <212> DNA <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic polynucleotide

<220> 10 <223> 2e12-sss-IgG1 HL SMIP (DNA)

<400> 200 atggattttc aagtgcagat tttcagcttc ctgctaatca gtgcttcagt cataatgtcc 60 15 agaggagtcc aggtgcagct gaaggagtca ggacctggcc tggtggcgcc ctcacagagc 120

ctgtccatca catgcaccgt ctcagggttc tcattaaccg gctatggtgt 20 aaactgggtt 180

cgccagcctc caggaaaggg tctagagtgg ctgggaatga tatggggtga tggaagcaca 240

25 gactataatt cagctctcaa atccagacta tcgatcacca aggacaactc caagagccaa 300

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccagatacta ctgtgctcga 360 30 gatggttata gtaactttca ttactatgtt atggactact ggggtcaagg aacctcagtc 420

accgtctcct ctgggggtgg aggctctggt ggcggtggat ccggcggagg 35 tgggtcgggt 480

ggcggcggat ctgacattgt gctcacccaa tctccagctt ctttggctgt

gtctctaggt 540

cagagagcca ccatctcctg cagagccagt gaaagtgttg aatattatgt cacaagttta 600 5 atgcagtggt accaacagaa accaggacag ccacccaaac tcctcatctc 2021201261

tgctgctagc 660

aacgtagaat ctggggtccc tgccaggttt agtggcagtg ggtctgggac 10 agactttagc 720

ctcaacatcc atcctgtgga ggaggatgat attgcaatgt atttctgtca gcaaagtagg 780

15 aaggttccat ggacgttcgg tggaggcacc aagctggaaa tcaaacgtga tcaggagccc 840

aaatcttctg acaaaactca cacatcccca ccgtccccag cacctgaact cctgggggga 900 20 tcgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct 960

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa 25 gttcaactgg 1020

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac 1080

30 agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag 1140

gagtacaagt gcaaggtctc caacaaagcc ctcccagcct ccatcgagaa aacaatctcc 1200 35 aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag 1260

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc 1320

5 gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg 1380 2021201261

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg 1440 10 cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg 1500

cagaagagcc tctccctgtc tccgggtaaa tga 15 1533

<210> 201 <211> 510 20 <212> PRT <213> Artificial sequence

<220> <223> Synthetic polypeptide 25 <220> <223> 2e12-sss-IgG1 HL SMIP (AA)

<220> 30 <221> misc_feature <222> (1)..(22) <223> Leader

<220> 35 <221> misc_feature <222> (24)..(144) <223> VH

<220> <221> misc_feature <222> (145)..(164) 5 <223> Linker 2021201261

<220> <221> misc_feature <222> (165)..(276) 10 <223> VL

<220> <221> misc_feature <222> (279)..(293) 15 <223> Hinge

<400> 201

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser 20 Ala Ser 1 5 10 15

Val Ile Met Ser Arg Gly Val Gln Val Gln Leu Lys Glu Ser 25 Gly Pro 20 25 30

Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr 30 Val Ser 35 40 45

Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln 35 Pro Pro 50 55 60

Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 65 70 75 5 80 2021201261

Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn 10 85 90 95

Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp 15 100 105 110

Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr 20 115 120 125

Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 25 130 135 140

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 30 145 150 155 160

Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser 35 Leu Ala 165 170

Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser 5 180 185 190 2021201261

Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 10 195 200 205

Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 15 210 215 220

Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 225 230 235 20 240

Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys 25 245 250 255

Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr 30 Lys Leu 260 265 270

Glu Ile Lys Arg Asp Gln Glu Pro Lys Ser Ser Asp Lys Thr 35 His Thr 275 280 285

Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Ser Ser Val Phe 290 295 300 5 2021201261

Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 305 310 315 10 320

Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 15 325 330 335

Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 20 Lys Thr 340 345 350

Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 25 Ser Val 355 360 365

Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 30 Lys Cys 370 375 380

Lys Val Ser Asn Lys Ala Leu Pro Ala Ser Ile Glu Lys Thr 35 Ile Ser 385 390 395

Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 5 405 410 415 2021201261

Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 10 Leu Val 420 425 430

Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 15 Asn Gly 435 440 445

Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 20 Ser Asp 450 455 460

Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 25 Arg Trp 465 470 475 480

Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 30 Leu His 485 490 495

35 Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 500 505 510

<210> 202 <211> 1518 <212> DNA 5 <213> Artificial sequence 2021201261

<220> <223> Synthetic polynucleotide

10 <220> <223> 2e12-sss-IgG1 LH SMIP (DNA)

<400> 202 atggattttc aagtgcagat tttcagcttc ctgctaatca gtgcttcagt 15 cataatgtcc 60

agaggagtcg acattgtgct cacccaatct ccagcttctt tggctgtgtc tctaggtcag 120

20 agagccacca tctcctgcag agccagtgaa agtgttgaat attatgtcac aagtttaatg 180

cagtggtacc aacagaaacc aggacagcca cccaaactcc tcatctctgc tgctagcaac 240 25 gtagaatctg gggtccctgc caggtttagt ggcagtgggt ctgggacaga ctttagcctc 300

aacatccatc ctgtggagga ggatgatatt gcaatgtatt tctgtcagca 30 aagtaggaag 360

gttccatgga cgttcggtgg aggcaccaag ctggaaatca aacggggtgg cggtggatcc 420

35 ggcggaggtg ggtcgggtgg cggcggatct caggtgcagc tgaaggagtc aggacctggc 480

ctggtggcgc cctcacagag cctgtccatc acatgcaccg tctcagggtt ctcattaacc 540

ggctatggtg taaactgggt tcgccagcct ccaggaaagg gtctagagtg 5 gctgggaatg 600 2021201261

atatggggtg atggaagcac agactataat tcagctctca aatccagact atcgatcacc 660

10 aaggacaact ccaagagcca agttttctta aaaatgaaca gtctgcaaac tgatgacaca 720

gccagatact actgtgctcg agatggttat agtaactttc attactatgt tatggactac 780 15 tggggtcaag gaacctcagt caccgtctcc tctgatcagg agcccaaatc ttctgacaaa 840

actcacacat ccccaccgtc cccagcacct gaactcctgg ggggaccgtc 20 agtcttcctc 900

ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg 960

25 gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt ggacggcgtg 1020

gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg 1080 30 gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcaag 1140

gtctccaaca aagccctccc agcccccatc gagaaaacaa tctccaaagc 35 caaagggcag 1200

ccccgagaac cacaggtgta caccctgccc ccatcccggg atgagctgac

caagaaccag 1260

gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt ggagtgggag 1320 5 agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga 2021201261

ctccgacggc 1380

tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca 10 ggggaacgtc 1440

ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa gagcctctcc 1500

15 ctgtctccgg gtaaatga 1518

<210> 203 20 <211> 505 <212> PRT <213> Artificial sequence

<220> 25 <223> Synthetic polypeptide

<220> <223> 2e12-sss-IgG1 LH SMIP (AA)

30 <220> <221> misc_feature <222> (1)..(23) <223> Leader

35 <220> <221> misc_feature <222> (24)..(135)

<223> VL

<220> <221> misc_feature 5 <222> (136)..(150) <223> Linker 2021201261

<220> <221> misc_feature 10 <222> (151)..(271) <223> VH

<220> <221> misc_feature 15 <222> (274)..(288) <223> Hinge

<400> 203

20 Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15

25 Val Ile Met Ser Arg Gly Val Asp Ile Val Leu Thr Gln Ser Pro Ala 20 25 30

30 Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala 35 40 45

35 Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln 50 55 60

Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 5 65 70 75 80 2021201261

Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser 10 Gly Thr 85 90 95

Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile 15 Ala Met 100 105 110

Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly 20 Gly Gly 115 120 125

Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly 25 Gly Gly 130 135 140

Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Lys Glu Ser Gly 30 Pro Gly 145 150 155 160

35 Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly 165 170

Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro 5 Pro Gly 180 185 190 2021201261

Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp 10 195 200 205

Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser 15 210 215 220

Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 20 225 230 235 240

Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His 25 Tyr Tyr 245 250 255

30 Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Asp 260 265 270

35 Gln Glu Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser Pro 275 280 285

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 5 290 295 300 2021201261

Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 10 305 310 315 320

Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn 15 Trp Tyr 325 330 335

20 Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 340 345 350

25 Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 355 360 365

30 Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 370 375 380

35 Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 385 390 395

Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 5 Glu Leu 405 410 2021201261

415

10 Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 420 425 430

15 Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 435 440 445

Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 20 Phe Leu 450 455 460

Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 25 Asn Val 465 470 475 480

30 Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 485 490 495

35 Lys Ser Leu Ser Leu Ser Pro Gly Lys 500 505

<210> 204 <211> 1498 5 <212> DNA <213> Artificial sequence 2021201261

<220> <223> Synthetic polynucleotide 10 <220> <223> G28-1 LH SMIP (DNA)

<400> 204 15 aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60

gataccaccg gtgacatcca gatgactcag tctccagcct ccctatctgc atctgtggga 120 20 gagactgtca ccatcacatg tcgaacaagt gaaaatgttt acagttattt ggcttggtat 180

cagcagaaac agggaaaatc tcctcagctc ctggtctctt ttgcaaaaac 25 cttagcagaa 240

ggtgtgccat caaggttcag tggcagtgga tcaggcacac agttttctct gaagatcagc 300

30 agcctgcagc ctgaagattc tggaagttat ttctgtcaac atcattccga taatccgtgg 360

acgttcggtg gaggcaccga actggagatc aaaggtggcg gtggctcggg cggtggtggg 420 35 tcgggtggcg gcggatctgc tagcgcagtc cagctgcagc agtctggacc tgagctggaa 480

aagcctggcg cttcagtgaa gatttcctgc aaggcttctg gttactcatt cactggctac 540

5 aatatgaact gggtgaagca gaataatgga aagagccttg agtggattgg aaatattgat 600 2021201261

ccttattatg gtggtactac ctacaaccgg aagttcaagg gcaaggccac attgactgta 660 10 gacaaatcct ccagcacagc ctacatgcag ctcaagagtc tgacatctga ggactctgca 720

gtctattact gtgcaagatc ggtcggccct atggactact ggggtcaagg 15 aacctcagtc 780

accgtctcga gcgagcccaa atcttctgac aaaactcaca catgcccacc gtgcccagca 840

20 cctgaactcc tgggtggacc gtcagtcttc ctcttccccc caaaacccaa ggacaccctc 900

atgatctccc ggacccctga ggtcacatgc gtggtggtgg acgtgagcca cgaagaccct 960 25 gaggtcaagt tcaactggta cgtggacggc gtggaggtgc ataatgccaa gacaaagccg 1020

cgggaggagc agtacaacag cacgtaccgt gtggtcagcg tcctcaccgt 30 cctgcaccag 1080

gactggctga atggcaagga gtacaagtgc aaggtctcca acaaagccct cccagccccc 1140 atcgagaaaa ccatctccaa agccaaaggg cagccccgag aaccacaggt 35 gtacaccctg 1200

cccccatccc gggatgagct gaccaagaac caggtcagcc tgacctgcct

ggtcaaaggc 1260

ttctatccaa gcgacatcgc cgtggagtgg gagagcaatg ggcagccgga gaacaactac 1320 5 aagaccacgc ctcccgtgct ggactccgac ggctccttct tcctctacag 2021201261

caagctcacc 1380

gtggacaaga gcaggtggca gcaggggaac gtcttctcat gctccgtgat 10 gcatgaggct 1440

ctgcacaacc actacacgca gaagagcctc tccctgtctc cgggtaagtg actctaga 1498

15 <210> 205 <211> 492 <212> PRT <213> Artificial sequence 20 <220> <223> Synthetic polypeptide

<220> 25 <223> G28-1 LH SMIP (AA)

<220> <221> misc_feature <222> (1)..(20) 30 <223> Leader

<220> <221> misc_feature <222> (21)..(127) 35 <223> VL <220> <221> misc_feature

<222> (128)..(144) <223> Linker

<220> 5 <221> misc_feature <222> (145)..(260) 2021201261

<223> VH

<220> 10 <221> misc_feature <222> (261)..(275) <223> Hinge

<400> 205 15 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 1 5 10 15

20 Asp Thr Thr Gly Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser 20 25 30

25 Ala Ser Val Gly Glu Thr Val Thr Ile Thr Cys Arg Thr Ser Glu Asn 35 40 45

30 Val Tyr Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro 50 55 60

35 Gln Leu Leu Val Ser Phe Ala Lys Thr Leu Ala Glu Gly Val Pro Ser

65 70 75 80

5 Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Phe Ser Leu Lys Ile Ser 2021201261

85 90 95

10 Ser Leu Gln Pro Glu Asp Ser Gly Ser Tyr Phe Cys Gln His His Ser 100 105 110

15 Asp Asn Pro Trp Thr Phe Gly Gly Gly Thr Glu Leu Glu Ile Lys Gly 115 120 125

20 Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser 130 135 140

25 Ala Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Glu Lys Pro Gly Ala 145 150 155 160

30 Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 165 170 175 35

Asn Met Asn Trp Val Lys Gln Asn Asn Gly Lys Ser Leu Glu

Trp Ile 180 185 190

5 Gly Asn Ile Asp Pro Tyr Tyr Gly Gly Thr Thr Tyr Asn Arg Lys Phe 2021201261

195 200 205

10 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 210 215 220

15 Met Gln Leu Lys Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 225 230 235 240

20 Ala Arg Ser Val Gly Pro Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 245 250 255 25

Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro 260 265 270 30

Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 275 280 285 35

Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro

Glu Val 290 295 300

5 Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 2021201261

305 310 315 320

10 Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 325 330 335 15

Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 340 345 350 20

Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 355 360 365 25

Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 370 375 380 30

Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 385 390 395 35 400

Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 405 410 415 5 Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 2021201261

Gln Pro 420 425 430

10 Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 435 440 445

15 Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 450 455 460

20 Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 465 470 475 480 25

Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490

30 <210> 206 <211> 1522 <212> DNA <213> Artificial sequence 35 <220> <223> Synthetic polynucleotide

<220> <223> G28-1 HL SMIP (DNA)

5 <400> 206 aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact 2021201261

ctggctccca 60

gataccaccg gtgcggtcca gctgcagcag tctggacctg agtcggaaaa 10 gcctggcgct 120

tcagtgaaga tttcctgcaa ggcttctggt tactcattca ctggctacaa tatgaactgg 180

15 gtgaagcaga ataatggaaa gagccttgag tggattggaa atattgatcc ttattatggt 240

ggtactacct acaaccggaa gttcaagggc aaggccacat tgactgtaga caaatcctcc 300 20 ggcacagcct acatgcagct caagagtctg acatctgagg actctgcagt ctattactgt 360

gcaagatcgg tcggccctat ggactactgg ggtcaaggaa cctcagtcac 25 cgtctcttct 420

ggtggcggtg gctcgggcgg tggtgggtcg ggtggcggcg gatcaggagg aggcgggagt 480

30 gctagcgaca tccagatgac tcagtctcca gcctccctat ctgcatctgt gggagagact 540

gtcaccatca catgtcgaac aagtgaaaat gtttacagtt atttggcttg gtatcagcag 600 35 aaacagggaa aatctcctca gctcctggtc tcttttgcaa aaaccttagc agaaggtgtg 660

ccatcaaggt tcagtggcag tggatcaggc acacagtttt ctctgaagat cagcagcctg 720

5 cagcctgaag attctggaag ttatttctgt caacatcatt ccgataatcc gtggacgttc 780 2021201261

ggtggaggca ccgaactgga gatcaaaggc tcgagcgagc ccaaatcttc tgacaaaact 840 10 cacacatgcc caccgtgccc agcacctgaa ctcctgggtg gaccgtcagt cttcctcttc 900

cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac 15 atgcgtggtg 960

gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag 1020

20 gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc 1080

agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc 1140 25 tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc 1200

cgagaaccac aggtgtacac cctgccccca tcccgggatg agctgaccaa 30 gaaccaggtc 1260

agcctgacct gcctggtcaa aggcttctat ccaagcgaca tcgccgtgga gtgggagagc 1320

35 aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc 1380

ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc 1440

tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag 5 cctctccctg 1500 2021201261

tctccgggta agtgactcta ga 1522

10 <210> 207 <211> 500 <212> PRT <213> Artificial sequence 15 <220> <223> Synthetic polypeptide

<220> 20 <223> G28-1 HL SMIP (AA)

<220> <221> misc_feature <222> (1)..(20) 25 <223> Leader

<220> <221> misc_feature <222> (21)..(136) 30 <223> VH

<220> <221> misc_feature <222> (137)..(158) 35 <223> Linker

<220>

<221> misc_feature <222> (159)..(266) <223> VL

5 <220> <221> misc_feature 2021201261

<222> (268)..(283) <223> Hinge

10 <400> 207

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 1 5 10 15 15

Asp Thr Thr Gly Ala Val Gln Leu Gln Gln Ser Gly Pro Glu Ser Glu 20 25 30 20

Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser 35 40 45 25

Phe Thr Gly Tyr Asn Met Asn Trp Val Lys Gln Asn Asn Gly Lys Ser 50 55 60 30

Leu Glu Trp Ile Gly Asn Ile Asp Pro Tyr Tyr Gly Gly Thr Thr Tyr 65 70 75 35 80

Asn Arg Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser 85 90 95

5 Gly Thr Ala Tyr Met Gln Leu Lys Ser Leu Thr Ser Glu Asp 2021201261

Ser Ala 100 105 110

10 Val Tyr Tyr Cys Ala Arg Ser Val Gly Pro Met Asp Tyr Trp Gly Gln 115 120 125

15 Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 130 135 140

20 Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Asp Ile 145 150 155 160

25 Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Ala Ser Val Gly Glu Thr 165 170 175 30

Val Thr Ile Thr Cys Arg Thr Ser Glu Asn Val Tyr Ser Tyr Leu Ala 180 185 190 35

Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val

Ser Phe 195 200 205

5 Ala Lys Thr Leu Ala Glu Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 2021201261

210 215 220

10 Ser Gly Thr Gln Phe Ser Leu Lys Ile Ser Ser Leu Gln Pro Glu Asp 225 230 235 240

15 Ser Gly Ser Tyr Phe Cys Gln His His Ser Asp Asn Pro Trp Thr Phe 245 250 255 20

Gly Gly Gly Thr Glu Leu Glu Ile Lys Gly Ser Ser Glu Pro Lys Ser 260 265 270 25

Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu 275 280 285 30

Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 290 295 300 35

Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp

Val Ser 305 310 315 320

5 His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 2021201261

Val Glu 325 330 335 10

Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr 340 345 350 15

Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 355 360 365 20

Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro 370 375 380 25

Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 385 390 395 30 400

Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 405 410 35 415

Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 420 425 430

5 Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 2021201261

Thr Pro 435 440 445

10 Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 450 455 460

15 Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 465 470 475 480 20

Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 485 490 25 495

Ser Pro Gly Lys 500 30

<210> 208 <211> 1522 <212> DNA 35 <213> Artificial sequence

<220>

<223> Synthetic polynucleotide

<220> <223> G19-4 LH SMIP (DNA) 5 <400> 208 2021201261

aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60

10 gataccaccg gtgacatcca gatgacacag actacatcct ccctgtctgc ctctctggga 120

gacagagtca ccatcagttg cagggcaagt caggacattc gcaattattt aaactggtat 180 15 cagcagaaac cagatggaac tgttaaactc ctgatctact acacatcaag attacactca 240

ggagtcccat caaggttcag tggcagtggg tctggaacag attattctct 20 caccattgcc 300

aacctgcaac cagaagatat tgccacttac ttttgccaac agggtaatac gcttccgtgg 360

25 acgttcggtg gaggcaccaa actggtaacc aaacggggtg gcggtggctc gggcggtggt 420

ggatctggag gaggtgggag cgctagcgag gtccagctgc aacagtctgg acctgaactg 480 30 gtgaagcctg gagcttcaat gaagatttcc tgcaaggcct ctggttactc attcactggc 540

tacatcgtga actggctgaa gcagagccat ggaaagaacc ttgagtggat 35 tggacttatt 600

aatccataca aaggtcttac tacctacaac cagaaattca agggcaaggc

cacattaact 660

gtagacaagt catccagcac agcctacatg gagctcctca gtctgacatc tgaagactct 720 5 gcagtctatt actgtgcaag atctgggtac tatggtgact cggactggta 2021201261

cttcgatgtc 780

tggggcgcag ggaccacggt caccgtctcc tcgagcgagc ccaaatcttc 10 tgacaaaact 840

cacacatgcc caccgtgccc agcacctgaa ctcctgggtg gaccgtcagt cttcctcttc 900

15 cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg 960

gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag 1020 20 gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc 1080

agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa 25 gtgcaaggtc 1140

tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc 1200

30 cgagaaccac aggtgtacac cctgccccca tcccgggatg agctgaccaa gaaccaggtc 1260

agcctgacct gcctggtcaa aggcttctat ccaagcgaca tcgccgtgga gtgggagagc 1320 35 aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc 1380

ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc 1440

5 tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag cctctccctg 1500 2021201261

tctccgggta agtgactcta ga 1522 10

<210> 209 <211> 500 <212> PRT 15 <213> Artificial sequence

<220> <223> Synthetic polypeptide

20 <220> <223> G19-4 LH SMIP (AA)

<220> <221> misc_feature 25 <222> (1)..(20) <223> Leader

<220> <221> misc_feature 30 <222> (21)..(128) <223> VL

<220> <221> misc_feature 35 <222> (129)..(145) <223> Linker

<220> <221> misc_feature <222> (146)..(267) <223> VH 5 <220> 2021201261

<221> misc_feature <222> (268)..(282) <223> Hinge 10 <400> 209

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 15 1 5 10 15

Asp Thr Thr Gly Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser 20 20 25 30

Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp 25 35 40 45

Ile Arg Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val 30 50 55 60

Lys Leu Leu Ile Tyr Tyr Thr Ser Arg Leu His Ser Gly Val Pro Ser 35 65 70 75

Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ala 85 90 95 5 2021201261

Asn Leu Gln Pro Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn 100 105 110 10

Thr Leu Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Val Thr Lys Arg 115 120 125 15

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala 130 135 140 20

Ser Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly 145 150 155 25 160

Ala Ser Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly 30 165 170 175

Tyr Ile Val Asn Trp Leu Lys Gln Ser His Gly Lys Asn Leu 35 Glu Trp 180 185 190

Ile Gly Leu Ile Asn Pro Tyr Lys Gly Leu Thr Thr Tyr Asn Gln Lys 195 200 205 5 2021201261

Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala 210 215 220 10

Tyr Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr 225 230 235 15 240

Cys Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr Phe Asp Val 20 245 250 255

Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser Ser Glu Pro 25 Lys Ser 260 265 270

Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 30 Leu Leu 275 280 285

Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 35 Thr Leu 290 295 300

Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 305 310 315 5 320 2021201261

His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu 10 325 330 335

Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 15 Ser Thr 340 345 350

Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 20 Leu Asn 355 360 365

Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 25 Ala Pro 370 375 380

Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 30 Pro Gln 385 390 395 400

35 Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val 405 410

Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 5 Ala Val 420 425 430 2021201261

Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 10 Thr Pro 435 440 445

Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 15 Leu Thr 450 455 460

Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 20 Ser Val 465 470 475 480

25 Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 485 490 495

30 Ser Pro Gly Lys 500

<210> 210 35 <211> 1525 <212> DNA <213> Artificial sequence

<220> <223> Synthetic polynucleotide

5 <220> <223> G19-4 HL SMIP (DNA) 2021201261

<400> 210 aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact 10 ctggctccca 60

gataccaccg gtgaggtcca gctgcaacag tctggacctg aactggtgaa gcctggagct 120

15 tcaatgaaga tttcctgcaa ggcctctggt tactcattca ctggctacat cgtgaactgg 180

ctgaagcaga gccatggaaa gaaccttgag tggattggac ttattaatcc atacaaaggt 240 20 cttactacct acaaccagaa attcaagggc aaggccacat tgactgtaga caagtcatcc 300

agcacagcct acatggagct cctcagtctg acatctgaag actctgcagt 25 ctattactgt 360

gcaagatctg ggtactatgg tgactcagac tggtacttcg atgtctgggg cgcagggacc 420

30 acagtcaccg tctcctctgg cggcggtggc tcgggcggtg gtggatctgg aggaggtggg 480

agcgctagcg acatccagat gacacagact acatcctccc tgtctgcctc tctgggagac 540 35 agagtcacca tcagttgcag ggcaagtcag gacattcgca attatttgaa ctggtatcag 600

cagaaaccag atggaactgt taaactcctg atctactaca catcaagact acactcagga 660

5 gtcccatcaa ggttcagtgg cagtgggtct ggaacagatt attctctcac cattgccaac 720 2021201261

ctgcaaccag aagatattgc cacttacttt tgccaacagg gtaatacact tccctggacg 780 10 ttcggtggag ggaccaaact ggtgaccaaa cgctcgagcg agcccaaatc ttctgacaaa 840

actcacacat gcccaccgtg cccagcacct gaactcctgg gtggaccgtc 15 agtcttcctc 900

ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt cacatgcgtg 960

20 gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt ggacggcgtg 1020

gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac gtaccgtgtg 1080 25 gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta caagtgcaag 1140

gtctccaaca aagccctccc agcccccatc gagaaaacca tctccaaagc 30 caaagggcag 1200

ccccgagaac cacaggtgta caccctgccc ccatcccggg atgagctgac caagaaccag 1260

35 gtcagcctga cctgcctggt caaaggcttc tatccaagcg acatcgccgt ggagtgggag 1320

agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc 1380

tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca 5 ggggaacgtc 1440 2021201261

ttctcatgct ccgtgatgca tgaggctctg cacaaccact acacgcagaa gagcctctcc 1500

10 ctgtctccgg gtaagtgact ctaga 1525

<210> 211 15 <211> 501 <212> PRT <213> Artificial sequence

<220> 20 <223> Synthetic polypeptide

<220> <223> G19-4 HL SMIP (AA)

25 <220> <221> misc_feature <222> (1)..(20) <223> Leader

30 <220> <221> misc_feature <222> (21)..(142) <223> VH

35 <220> <221> misc_feature <222> (143)..(159)

<223> Linker

<220> <221> misc_feature 5 <222> (160)..(267) <223> VL 2021201261

<220> <221> misc_feature 10 <222> (270)..(284) <223> Hinge

<400> 211

15 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 1 5 10 15

20 Asp Thr Thr Gly Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val 20 25 30

25 Lys Pro Gly Ala Ser Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser 35 40 45

30 Phe Thr Gly Tyr Ile Val Asn Trp Leu Lys Gln Ser His Gly Lys Asn 50 55 60

35 Leu Glu Trp Ile Gly Leu Ile Asn Pro Tyr Lys Gly Leu Thr Thr Tyr 65 70 75

Asn Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser 5 85 90 95 2021201261

Ser Thr Ala Tyr Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala 10 100 105 110

Val Tyr Tyr Cys Ala Arg Ser Gly Tyr Tyr Gly Asp Ser Asp Trp Tyr 15 115 120 125

Phe Asp Val Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser Gly Gly 20 130 135 140

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser Asp 25 145 150 155 160

Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu 30 Gly Asp 165 170 175

35 Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Arg Asn Tyr Leu 180 185 190

Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile Tyr 5 195 200 205 2021201261

Tyr Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 10 210 215 220

Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ala Asn Leu Gln Pro Glu 15 225 230 235 240

Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro 20 Trp Thr 245 250 255

25 Phe Gly Gly Gly Thr Lys Leu Val Thr Lys Arg Ser Ser Glu Pro Lys 260 265 270

30 Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu 275 280 285

35 Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 290 295 300

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 5 305 310 315 320 2021201261

Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 10 Gly Val 325 330 335

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 15 Asn Ser 340 345 350

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 20 Trp Leu 355 360 365

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 25 Pro Ala 370 375 380

Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 30 Glu Pro 385 390 395 400

35 Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln 405 410

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 5 Ile Ala 420 425 430 2021201261

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 10 Thr Thr 435 440 445

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 15 Lys Leu 450 455 460

Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 20 Cys Ser 465 470 475 480

25 Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 485 490 495

30 Leu Ser Pro Gly Lys 500

35 <210> 212 <211> 2328 <212> DNA

<213> Artificial sequence

<220> <223> Synthetic polynucleotide 5 <220> 2021201261

<223> n2H7sssIgG1-STD1-2e12HL (DNA)

<400> 212 10 aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60

gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg 120 15 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180

cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct 20 ggcttctgga 240

gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac aatcagcaga 300

25 gtggaggctg aagatgctgc cacttattac tgccagcagt ggagttttaa cccacccacg 360

ttcggtgctg ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420 30 ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480

ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag 35 ttacaatatg 540

cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat

ttatccagga 600

aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac tgtagacaaa 660 5 tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc 2021201261

tgcggtctat 720

ttctgtgcaa gagtggtgta ctatagtaac tcttactggt acttcgatgt 10 ctggggcaca 780

gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca 840

15 ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900

aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 960 20 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc 1020

aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag 25 cgtcctcacc 1080

gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140

30 ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag 1200

gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260 35 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg 1320

gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac 1380

5 agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440 2021201261

atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500 10 aattatggtg gcggtggctc gggcggtggt ggatctggag gaggtgggag tgggaattct 1560

caggtgcagc tgaaggagtc aggacctggc ctggtggcgc cctcacagag 15 cctgtccatc 1620

acatgcaccg tctcagggtt ctcattaacc ggctatggtg taaactgggt tcgccagcct 1680

20 ccaggaaagg gtctggagtg gctgggaatg atatggggtg atggaagcac agactataat 1740

tcagctctca aatccagact atcgatcacc aaggacaact ccaagagcca agttttctta 1800 25 aaaatgaaca gtctgcaaac tgatgacaca gccagatact actgtgctcg agatggttat 1860

agtaactttc attactatgt tatggactac tggggtcaag gaacctcagt 30 caccgtctcc 1920

tctgggggtg gaggctctgg tggcggtgga tccggcggag gtgggtcggg tggcggcgga 1980

35 tctgacattg tgctcaccca atctccagct tctttggctg tgtctctagg tcagagagcc 2040

accatctcct gcagagccag tgaaagtgtt gaatattatg tcacaagttt aatgcagtgg 2100

taccaacaga aaccaggaca gccacccaaa ctcctcatct ctgctgctag 5 caacgtagaa 2160 2021201261

tctggggtcc ctgccaggtt tagtggcagt gggtctggga cagactttag cctcaacatc 2220

10 catcctgtgg aggaggatga tattgcaatg tatttctgtc agcaaagtag gaaggttcca 2280

tggacgttcg gtggaggcac caagctggaa atcaaacgtt aatctaga 2328 15

<210> 213 <211> 769 <212> PRT 20 <213> Artificial sequence

<220> <223> Synthetic polypeptide

25 <220> <223> n2H7sssIgG1-STD1-2e12HL (AA)

<220> 30 <221> misc_feature <222> (1)..(20) <223> Leader

<220> 35 <221> misc_feature <222> (21)..(127) <223> VL

<220> <221> misc_feature <222> (128)..(142) 5 <223> Linker 2021201261

<220> <221> misc_feature <222> (143)..(264) 10 <223> VH

<220> <221> misc_feature <222> (265)..(279) 15 <223> Hinge

<220> <221> misc_feature <222> (497)..(516) 20 <223> EFD-BD2 Linker

<220> <221> misc_feature <222> (517)..(637) 25 <223> VH2

<220> <221> misc_feature <222> (638)..(657) 30 <223> Linker2

<220> <221> misc_feature <222> (658)..(769) 35 <223> VL2

<400> 213

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 1 5 10 15 5 2021201261

Asp Thr Thr Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 10

Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 15

Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60 20

Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 25 80

Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 30 85 90 95

Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe 35 100 105 110

Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Asp Gly 115 120 125

5 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ala Ser 2021201261

Gln Ala 130 135 140

10 Tyr Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala Ser Val 145 150 155 160 15

Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 20 175

His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 25 180 185 190

Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 30 195 200 205

Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 35 210 215 220

Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg 225 230 235 240 5 2021201261

Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp Gly Thr 245 250 10 255

Gly Thr Thr Val Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr 15 260 265 270

His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 20 275 280 285

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 25 290 295 300

Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 30 305 310 315 320

Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 35 Asn Ala 325 330

Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 5 340 345 350 2021201261

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 10 355 360 365

Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 15 370 375 380

Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 20 385 390 395 400

Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 25 Thr Cys 405 410 415

30 Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430

35 Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445

Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455 460 5 2021201261

Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 465 470 475 10 480

Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 15 485 490 495

Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 20 Gly Gly 500 505 510

Ser Gly Asn Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly 25 Leu Val 515 520 525

Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly 30 Phe Ser 530 535 540

Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly 35 Lys Gly 545 550 555

Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn 5 565 570 575 2021201261

Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser 10 Lys Ser 580 585 590

Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr 15 Ala Arg 595 600 605

Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr 20 Val Met 610 615 620

Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly 25 Gly Gly 625 630 635 640

30 Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 645 650 655

35 Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu

660 665 670

Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val 5 Glu Tyr 675 680 685 2021201261

Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly 10 Gln Pro 690 695 700

Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro 15 705 710 715 720

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu 20 Asn Ile 725 730 735

25 His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser 740 745 750

30 Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 755 760 765

35 Arg

<210> 214 <211> 2313 <212> DNA 5 <213> Artificial sequence 2021201261

<220> <223> Synthetic polynucleotide

10 <220> <223> n2H7sssIgG1-STD2-2e12LH (DNA)

<400> 214 aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact 15 ctggctccca 60

gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg 120

20 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180

cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga 240 25 gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac aatcagcaga 300

gtggaggctg aagatgctgc cacttattac tgccagcagt ggagttttaa 30 cccacccacg 360

ttcggtgctg ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420

35 ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480

ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag ttacaatatg 540

cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat 5 ttatccagga 600 2021201261

aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac tgtagacaaa 660

10 tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc tgcggtctat 720

ttctgtgcaa gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780 15 gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca 840

ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc 20 cccaaaaccc 900

aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 960

25 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc 1020

aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 1080 30 gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140

ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg 35 agaaccacag 1200

gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag

cctgacctgc 1260

ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg 1320 5 gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt 2021201261

cttcctctac 1380

agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc 10 atgctccgtg 1440

atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500

15 aattatggtg gcggtggctc gggcggtggt ggatctggag gaggtgggag tgggaattct 1560

gacattgtgc tcacccaatc tccagcttct ttggctgtgt ctctaggtca gagagccacc 1620 20 atctcctgca gagccagtga aagtgttgaa tattatgtca caagtttaat gcagtggtac 1680

caacagaaac caggacagcc acccaaactc ctcatctctg ctgctagcaa 25 cgtagaatct 1740

ggggtccctg ccaggtttag tggcagtggg tctgggacag actttagcct caacatccat 1800

30 cctgtggagg aggatgatat tgcaatgtat ttctgtcagc aaagtaggaa ggttccatgg 1860

acgttcggtg gaggcaccaa gctggaaatc aaacggggtg gcggtggatc cggcggaggt 1920 35 gggtcgggtg gcggcggatc tcaggtgcag ctgaaggagt caggacctgg cctggtggcg 1980

ccctcacaga gcctgtccat cacatgcacc gtctcagggt tctcattaac cggctatggt 2040

5 gtaaactggg ttcgccagcc tccaggaaag ggtctggagt ggctgggaat gatatggggt 2100 2021201261

gatggaagca cagactataa ttcagctctc aaatccagac tatcgatcac caaggacaac 2160 10 tccaagagcc aagttttctt aaaaatgaac agtctgcaaa ctgatgacac agccagatac 2220

tactgtgctc gagatggtta tagtaacttt cattactatg ttatggacta 15 ctggggtcaa 2280

ggaacctcag tcaccgtctc ctcttaatct aga 2313

20 <210> 215 <211> 764 <212> PRT <213> Artificial sequence 25 <220> <223> Synthetic polypeptide

<220> 30 <223> n2H7sssIgG1-STD2-2e12LH (AA)

<220> <221> misc_feature <222> (1)..(20) 35 <223> Leader

<220>

<221> misc_feature <222> (21)..(126) <223> VL

5 <220> <221> misc_feature 2021201261

<222> (127)..(142) <223> Linker

10 <220> <221> misc_feature <222> (143)..(264) <223> VH

15 <220> <221> misc_feature <222> (265)..(279) <223> Hinge

20 <220> <221> misc_feature <222> (497)..(516) <223> EFD-BD2 Linker

25 <220> <221> misc_feature <222> (517)..(628) <223> VL2

30 <220> <221> misc_feature <222> (629)..(643) <223> Linker

35 <220> <221> misc_feature <222> (644)..(764)

<223> VH2

<400> 215

5 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 2021201261

1 5 10 15

10 Asp Thr Thr Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30

15 Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45

20 Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60

25 Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80

30 Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85 90 95

35 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe

100 105 110

Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 5 Asp Gly 115 120 125 2021201261

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ala Ser 10 Gln Ala 130 135 140

Tyr Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 15 Ser Val 145 150 155 160

20 Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175

25 His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190

30 Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205

35 Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln

210 215 220

Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 5 Ala Arg 225 230 235 2021201261

240

10 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp Gly Thr 245 250 255

15 Gly Thr Thr Val Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr 260 265 270

20 His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285

25 Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300

30 Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320

35 Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala

325 330 335

5 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 2021201261

340 345 350

10 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 355 360 365

15 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 370 375 380

20 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 385 390 395 400

25 Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 415 30

Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 35

Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val

Leu Asp 435 440 445

5 Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 2021201261

450 455 460

10 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 465 470 475 480

15 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490 495 20

Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 500 505 510 25

Ser Gly Asn Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala 515 520 525 30

Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser 530 535 540 35 Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro

545 550 555 560

5 Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 2021201261

565 570 575

10 Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser 580 585 590

15 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys 595 600 605

20 Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 610 615 620

25 Glu Ile Lys Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 625 630 635 640 30

Gly Gly Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala 645 650 35 655

Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu 660 665 670

5 Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys 2021201261

Gly Leu 675 680 685

10 Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser 690 695 700

15 Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln 705 710 715 720 20

Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr 725 730 25 735

Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp 30 740 745 750

Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 755 760 35

<210> 216

<211> 2048 <212> DNA <213> Artificial sequence

5 <220> <223> Synthetic polynucleotide 2021201261

<220> <223> n2H7sssIgG1-H1-2e12HL (DNA) 10 <400> 216 aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60

15 gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg 120

gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180 20 cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga 240

gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac 25 aatcagcaga 300

gtggaggctg aagatgctgc cacttattac tgccagcagt ggagttttaa cccacccacg 360

30 ttcggtgctg ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420

ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480 35 ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag ttacaatatg 540

cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga 600

5 aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac tgtagacaaa 660 2021201261

tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc tgcggtctat 720 10 ttctgtgcaa gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780

gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca 15 cacatcccca 840

ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900

20 aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 960

cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc 1020 25 aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 1080

gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc 30 caacaaagcc 1140

ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag 1200

35 gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260

ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg 1320

gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt 5 cttcctctac 1380 2021201261

agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440

10 atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500

aattctcagg tgcagctgaa ggagtcagga cctggcctgg tggcgccctc acagagcctg 1560 15 tccatcacat gcaccgtctc agggttctca ttaaccggct atggtgtaaa ctgggttcgc 1620

cagcctccag gaaagggtct ggagtggctg ggaatgatat ggggtgatgg 20 aagcacagac 1680

tataattcag ctctcaaatc cagactatcg atcaccaagg acaactccaa gagccaagtt 1740

25 ttcttaaaaa tgaacagtct gcaaactgat gacacagcca gatactactg tgctcgagat 1800

ggttatagta actttcatta ctatgttatg gactactggg gtcaaggaac ctcagtcacc 1860 30 gtctcctctg ggggtggagg ctctggtggc ggtggatccg gcggaggtgg gtcgggtggc 1920

ggcggatctg acattgtgct cacccaatct ccagcttctt tggctgtgtc 35 tctaggtcag 1980

agagccacca tctcctgcag agccagtgaa agtgttgaat attatgtcac

aagtttaatg 2040

cagtggta 2048 5 2021201261

<210> 217 <211> 751 <212> PRT 10 <213> Artificial sequence

<220> <223> Synthetic polypeptide

15 <220> <223> n2H7sssIgG1-H1-2e12HL (AA)

<220> <221> misc_feature 20 <222> (1)..(20) <223> Leader

<220> <221> misc_feature 25 <222> (21)..(126) <223> VL

<220> <221> misc_feature 30 <222> (127)..(142) <223> Linker

<220> <221> misc_feature 35 <222> (143)..(264) <223> VH

<220> <221> misc_feature <222> (265)..(279) <223> Hinge 5 <220> 2021201261

<221> misc_feature <222> (497)..(498) <223> EFD-BD2 Linker 10 <220> <221> misc_feature <222> (499)..(619) <223> VH2 15 <220> <221> misc_feature <222> (620)..(639) <223> Linker2 20 <220> <221> misc_feature <222> (640)..(751) <223> VL2 25 <400> 217

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 30 1 5 10 15

Asp Thr Thr Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser 35 20 25 30

Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45

5 Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 2021201261

Pro Lys 50 55 60

10 Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80 15

Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85 90 95 20

Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe 100 105 110 25

Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Asp Gly 115 120 125 30

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ala Ser Gln Ala 130 135 140 35

Tyr Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala

Ser Val 145 150 155 160

5 Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 2021201261

Asn Met 165 170 175 10

His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 15

Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205 20

Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210 215 220 25

Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg 225 230 235 30 240

Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp Gly Thr 35 245 250

Gly Thr Thr Val Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr 260 265 270 5 2021201261

His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 10

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300 15

Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 20 320

Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 25 325 330 335

Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 30 Val Val 340 345 350

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 35 Glu Tyr 355 360 365

Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 370 375 380 5 2021201261

Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 385 390 395 10 400

Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 15 405 410 415

Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 20 Glu Ser 420 425 430

Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 25 435 440 445

Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 30 450 455 460

Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 35 465 470 475

Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490 5 495 2021201261

Asn Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro 10 500 505 510

Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr 15 515 520 525

Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu 20 530 535 540

Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala 25 545 550 555 560

Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser 30 Gln Val 565 570 575

35 Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr 580 585 590

Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr 5 595 600 605 2021201261

Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser 10 610 615 620

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 15 625 630 635 640

Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu 20 Gly Gln 645 650 655

25 Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val 660 665 670

30 Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys 675 680 685

Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro 35 Ala Arg 690 695 700

Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro 705 710 715 5 720 2021201261

Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys 10 725 730 735

Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 15 Arg 740 745 750

<210> 218 20 <211> 2292 <212> DNA <213> Artificial sequence

<220> 25 <223> Synthetic polynucleotide

<220> <223> n2H7sssIgG1-H2-2e12HL (DNA)

30 <400> 218 aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60

gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc 35 atctccaggg 120

gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca

ctggtaccag 180

cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga 240 5 gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac 2021201261

aatcagcaga 300

gtggaggctg aagatgctgc cacttattac tgccagcagt ggagttttaa 10 cccacccacg 360

ttcggtgctg ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420

15 ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480

ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag ttacaatatg 540 20 cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga 600

aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac 25 tgtagacaaa 660

tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc tgcggtctat 720

30 ttctgtgcaa gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780

gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca 840 35 ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900

aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 960

5 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc 1020 2021201261

aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 1080 10 gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140

ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg 15 agaaccacag 1200

gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260

20 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg 1320

gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac 1380 25 agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440

atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc 30 tccgggtaag 1500

ggtggcggtg gctcggggaa ttctcaggtg cagctgaagg agtcaggacc tggcctggtg 1560

35 gcgccctcac agagcctgtc catcacatgc accgtctcag ggttctcatt aaccggctat 1620

ggtgtaaact gggttcgcca gcctccagga aagggtctgg agtggctggg aatgatatgg 1680

ggtgatggaa gcacagacta taattcagct ctcaaatcca gactatcgat 5 caccaaggac 1740 2021201261

aactccaaga gccaagtttt cttaaaaatg aacagtctgc aaactgatga cacagccaga 1800

10 tactactgtg ctcgagatgg ttatagtaac tttcattact atgttatgga ctactggggt 1860

caaggaacct cagtcaccgt ctcctctggg ggtggaggct ctggtggcgg tggatccggc 1920 15 ggaggtgggt cgggtggcgg cggatctgac attgtgctca cccaatctcc agcttctttg 1980

gctgtgtctc taggtcagag agccaccatc tcctgcagag ccagtgaaag 20 tgttgaatat 2040

tatgtcacaa gtttaatgca gtggtaccaa cagaaaccag gacagccacc caaactcctc 2100

25 atctctgctg ctagcaacgt agaatctggg gtccctgcca ggtttagtgg cagtgggtct 2160

gggacagact ttagcctcaa catccatcct gtggaggagg atgatattgc aatgtatttc 2220 30 tgtcagcaaa gtaggaaggt tccatggacg ttcggtggag gcaccaagct ggaaatcaaa 2280

cgttaatcta ga 35 2292

<210> 219 <211> 757 <212> PRT <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic polypeptide

<220> 10 <223> n2H7sssIgG1-H2-2e12HL (AA)

<220> <221> misc_feature <222> (1)..(20) 15 <223> Leader

<220> <221> misc_feature <222> (21)..(126) 20 <223> VL

<220> <221> misc_feature <222> (127)..(142) 25 <223> Linker

<220> <221> misc_feature <222> (143)..(264) 30 <223> VH

<220> <221> misc_feature <222> (265)..(279) 35 <223> Hinge

<220>

<221> misc_feature <222> (497)..(504) <223> EFD-BD2 Linker

5 <220> <221> misc_feature 2021201261

<222> (505)..(625) <223> VH2

10 <220> <221> misc_feature <222> (626)..(645) <223> Linker2

15 <220> <221> misc_feature <222> (646)..(757) <223> VL2

20 <400> 219

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 1 5 10 15 25

Asp Thr Thr Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30 30

Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45 35

Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser

Pro Lys 50 55 60

5 Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 2021201261

65 70 75 80

10 Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85 90 95

15 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe 100 105 110

20 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Asp Gly 115 120 125

25 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ala Ser Gln Ala 130 135 140

30 Tyr Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala Ser Val 145 150 155 160 35

Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr

Asn Met 165 170 175

5 His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 2021201261

Gly Ala 180 185 190

10 Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205

15 Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210 215 220

20 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg 225 230 235 240 25

Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp Gly Thr 245 250 30 255

Gly Thr Thr Val Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr 35 260 265 270

His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285

5 Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 2021201261

Ser Arg 290 295 300

10 Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 320

15 Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330 335 20

Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 340 345 350 25

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 355 360 365 30

Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 370 375 380 35

Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr

Thr Leu 385 390 395 400

5 Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 2021201261

Thr Cys 405 410 415 10

Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430 15

Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445 20

Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455 460 25

Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 465 470 475 30 480

Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 35 485 490

Gly Gly Gly Gly Ser Gly Asn Ser Gln Val Gln Leu Lys Glu Ser Gly 500 505 510 5 2021201261

Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val 515 520 525 10

Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro 530 535 540 15

Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser 545 550 555 20 560

Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp 565 570 25 575

Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr Asp 30 580 585 590

Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His 35 595 600 605

Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser 610 615 620

5 Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 2021201261

Gly Ser 625 630 635 640 10

Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu 645 650 15 655

Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu 20 660 665 670

Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys 25 675 680 685

Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu 30 690 695 700

Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 35 705 710 715

Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe 725 730 5 735 2021201261

Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys 10 740 745 750

Leu Glu Ile Lys Arg 755 15

<210> 220 <211> 2298 <212> DNA 20 <213> Artificial sequence

<220> <223> Synthetic polynucleotide

25 <220> <223> n2H7sssIgG1-H3-2e12HL (DNA)

<400> 220 aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact 30 ctggctccca 60

gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg 120

35 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180

cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga 240

gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac 5 aatcagcaga 300 2021201261

gtggaggctg aagatgctgc cacttattac tgccagcagt ggagttttaa cccacccacg 360

10 ttcggtgctg ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420

ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480 15 ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag ttacaatatg 540

cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat 20 ttatccagga 600

aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac tgtagacaaa 660

25 tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc tgcggtctat 720

ttctgtgcaa gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780 30 gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca 840

ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc 35 cccaaaaccc 900

aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt

ggacgtgagc 960

cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc 1020 5 aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag 2021201261

cgtcctcacc 1080

gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc 10 caacaaagcc 1140

ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag 1200

15 gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260

ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg 1320 20 gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac 1380

agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc 25 atgctccgtg 1440

atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500

30 aattatggtg gcggtggctc tgggaattct caggtgcagc tgaaggagtc aggacctggc 1560

ctggtggcgc cctcacagag cctgtccatc acatgcaccg tctcagggtt ctcattaacc 1620 35 ggctatggtg taaactgggt tcgccagcct ccaggaaagg gtctggagtg gctgggaatg 1680

atatggggtg atggaagcac agactataat tcagctctca aatccagact atcgatcacc 1740

5 aaggacaact ccaagagcca agttttctta aaaatgaaca gtctgcaaac tgatgacaca 1800 2021201261

gccagatact actgtgctcg agatggttat agtaactttc attactatgt tatggactac 1860 10 tggggtcaag gaacctcagt caccgtctcc tctgggggtg gaggctctgg tggcggtgga 1920

tccggcggag gtgggtcggg tggcggcgga tctgacattg tgctcaccca 15 atctccagct 1980

tctttggctg tgtctctagg tcagagagcc accatctcct gcagagccag tgaaagtgtt 2040

20 gaatattatg tcacaagttt aatgcagtgg taccaacaga aaccaggaca gccacccaaa 2100

ctcctcatct ctgctgctag caacgtagaa tctggggtcc ctgccaggtt tagtggcagt 2160 25 gggtctggga cagactttag cctcaacatc catcctgtgg aggaggatga tattgcaatg 2220

tatttctgtc agcaaagtag gaaggttcca tggacgttcg gtggaggcac 30 caagctggaa 2280

atcaaacgtt aatctaga 2298

35 <210> 221 <211> 759

<212> PRT <213> Artificial sequence

<220> 5 <223> Synthetic polypeptide 2021201261

<220> <223> n2H7sssIgG1-H3-2e12HL (AA)

10 <220> <221> misc_feature <222> (1)..(20) <223> Leader

15 <220> <221> misc_feature <222> (21)..(126) <223> VL

20 <220> <221> misc_feature <222> (127)..(142) <223> Linker

25 <220> <221> misc_feature <222> (143)..(264) <223> VH

30 <220> <221> misc_feature <222> (265)..(279) <223> Hinge

35 <220> <221> misc_feature <222> (497)..(506)

<223> EFD-BD2 Linker

<220> <221> misc_feature 5 <222> (507)..(627) <223> VH2 2021201261

<220> <221> misc_feature 10 <222> (628)..(647) <223> Linker2

<220> <221> misc_feature 15 <222> (648)..(759) <223> VL2

<400> 221

20 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 1 5 10 15

25 Asp Thr Thr Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30

30 Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45

35 Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60

Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 5 65 70 75 80 2021201261

Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 10 Ser Arg 85 90 95

Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp 15 Ser Phe 100 105 110

Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 20 Asp Gly 115 120 125

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ala Ser 25 Gln Ala 130 135 140

Tyr Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala 30 Ser Val 145 150 155 160

35 Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170

His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 5 Gly Ala 180 185 190 2021201261

Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 10 Lys Gly 195 200 205

Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 15 Met Gln 210 215 220

Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 20 Ala Arg 225 230 235 240

25 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp Gly Thr 245 250 255

30 Gly Thr Thr Val Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr 260 265 270

35 His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser

275 280 285

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 5 Ser Arg 290 295 300 2021201261

Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 10 Asp Pro 305 310 315 320

15 Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330 335

20 Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 340 345 350

25 Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 355 360 365

30 Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 370 375 380

35 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu

385 390 395 400

5 Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 2021201261

405 410 415

10 Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 420 425 430

15 Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445

20 Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455 460

25 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 465 470 475 480

30 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490 495 35

Asn Tyr Gly Gly Gly Gly Ser Gly Asn Ser Gln Val Gln Leu

Lys Glu 500 505 510

5 Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys 2021201261

515 520 525

10 Thr Val Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg 530 535 540

15 Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp 545 550 555 560

20 Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr 565 570 575 25

Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 580 585 590 30

Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn 595 600 605 35

Phe His Tyr Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser

Val Thr 610 615 620

5 Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 2021201261

625 630 635 640

10 Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala 645 650 655 15

Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala 660 665 670 20 Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln 675 680 685

25 Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn 690 695 700

30 Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr 705 710 715 720 35

Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile

Ala Met 725 730 735

5 Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly 2021201261

Gly Gly 740 745 750

10 Thr Lys Leu Glu Ile Lys Arg 755

15 <210> 222 <211> 2307 <212> DNA <213> Artificial sequence

20 <220> <223> Synthetic polynucleotide

<220> <223> n2H7sssIgG1-H4-2e12HL (DNA) 25 <400> 222 aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60

30 gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg 120

gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180 35 cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga 240

gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac aatcagcaga 300

5 gtggaggctg aagatgctgc cacttattac tgccagcagt ggagttttaa cccacccacg 360 2021201261

ttcggtgctg ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg tggtggatct 420 10 ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480

ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag 15 ttacaatatg 540

cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga 600

20 aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac tgtagacaaa 660

tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc tgcggtctat 720 25 ttctgtgcaa gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780

gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca 30 cacatcccca 840

ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900

35 aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 960

cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc 1020

aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag 5 cgtcctcacc 1080 2021201261

gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140

10 ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag 1200

gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260 15 ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg 1320

gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt 20 cttcctctac 1380

agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440

25 atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500

ggtggcggtg gctcgggcgg tggtggatct gggaattctc aggtgcagct gaaggagtca 1560 30 ggacctggcc tggtggcgcc ctcacagagc ctgtccatca catgcaccgt ctcagggttc 1620

tcattaaccg gctatggtgt aaactgggtt cgccagcctc caggaaaggg 35 tctggagtgg 1680

ctgggaatga tatggggtga tggaagcaca gactataatt cagctctcaa

atccagacta 1740

tcgatcacca aggacaactc caagagccaa gttttcttaa aaatgaacag tctgcaaact 1800 5 gatgacacag ccagatacta ctgtgctcga gatggttata gtaactttca 2021201261

ttactatgtt 1860

atggactact ggggtcaagg aacctcagtc accgtctcct ctgggggtgg 10 aggctctggt 1920

ggcggtggat ccggcggagg tgggtcgggt ggcggcggat ctgacattgt gctcacccaa 1980

15 tctccagctt ctttggctgt gtctctaggt cagagagcca ccatctcctg cagagccagt 2040

gaaagtgttg aatattatgt cacaagttta atgcagtggt accaacagaa accaggacag 2100 20 ccacccaaac tcctcatctc tgctgctagc aacgtagaat ctggggtccc tgccaggttt 2160

agtggcagtg ggtctgggac agactttagc ctcaacatcc atcctgtgga 25 ggaggatgat 2220

attgcaatgt atttctgtca gcaaagtagg aaggttccat ggacgttcgg tggaggcacc 2280

30 aagctggaaa tcaaacgtta atctaga 2307

<210> 223 35 <211> 762 <212> PRT <213> Artificial sequence

<220> <223> Synthetic polypeptide

5 <220> <223> n2H7sssIgG1-H4-2e12HL (AA) 2021201261

<220> <221> misc_feature 10 <222> (1)..(20) <223> Leader

<220> <221> misc_feature 15 <222> (21)..(126) <223> VL

<220> <221> misc_feature 20 <222> (127)..(142) <223> Linker

<220> <221> misc_feature 25 <222> (143)..(264) <223> VH

<220> <221> misc_feature 30 <222> (265)..(279) <223> Hinge

<220> <221> misc_feature 35 <222> (497)..(509) <223> EFD-BD2 Linker

<220> <221> misc_feature <222> (510)..(630) <223> VH2 5 <220> 2021201261

<221> misc_feature <222> (631)..(650) <223> Linker2 10 <220> <221> misc_feature <222> (651)..(762) <223> VL2 15 <400> 223

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 20 1 5 10 15

Asp Thr Thr Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser 25 20 25 30

Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser 30 35 40 45

Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 35 50 55 60

Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 65 70 75 80 5 2021201261

Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 85 90 95 10

Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe 100 105 110 15

Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Asp Gly 115 120 125 20

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ala Ser Gln Ala 130 135 140 25

Tyr Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala Ser Val 145 150 155 30 160

Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 35 165 170

His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190 5 2021201261

Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 195 200 205 10

Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210 215 220 15

Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg 225 230 235 20 240

Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp Gly Thr 25 245 250 255

Gly Thr Thr Val Thr Val Ser Ser Glu Pro Lys Ser Ser Asp 30 Lys Thr 260 265 270

His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 35 275 280 285

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 290 295 300

5 Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 2021201261

Asp Pro 305 310 315 320 10

Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330 15 335

Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 20 340 345 350

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 25 355 360 365

Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 30 370 375 380

Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 35 385 390 395

Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410 5 415 2021201261

Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 10 420 425 430

Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 15 435 440 445

Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 20 450 455 460

Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 25 465 470 475 480

Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 30 Gly Lys 485 490 495

35 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Asn Ser Gln Val Gln 500 505 510

Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser 5 515 520 525 2021201261

Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn 530 535 540 10

Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile 545 550 555 15 560

Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu 20 565 570 575

Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 25 Met Asn 580 585 590

Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg 30 Asp Gly 595 600 605

Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly Gln 35 Gly Thr 610 615 620

Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 625 630 635 5 640 2021201261

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln 10 645 650 655

Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr 15 Ile Ser 660 665 670

Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu 20 Met Gln 675 680 685

Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 25 Ser Ala 690 695 700

Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly 30 Ser Gly 705 710 715 720

35 Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu Asp Asp 725 730

Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp 5 Thr Phe 740 745 750 2021201261

Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 10 755 760

<210> 224 <211> 2313 15 <212> DNA <213> Artificial sequence

<220> <223> Synthetic polynucleotide 20 <220> <223> n2H7sssIgG1-H5-2e12HL (DNA)

<400> 224 25 aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60

gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg 120 30 gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca ctggtaccag 180

cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct 35 ggcttctgga 240

gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac

aatcagcaga 300

gtggaggctg aagatgctgc cacttattac tgccagcagt ggagttttaa cccacccacg 360 5 ttcggtgctg ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg 2021201261

tggtggatct 420

ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct 10 ggtgaggcct 480

ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag ttacaatatg 540

15 cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga 600

aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac tgtagacaaa 660 20 tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc tgcggtctat 720

ttctgtgcaa gagtggtgta ctatagtaac tcttactggt acttcgatgt 25 ctggggcaca 780

gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca 840

30 ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900

aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 960 35 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc 1020

aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc 1080

5 gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140 2021201261

ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg agaaccacag 1200 10 gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag cctgacctgc 1260

ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa 15 tgggcagccg 1320

gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac 1380

20 agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440

atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500 25 aattatggtg gcggtggctc gggcggtggt ggatctggga attctcaggt gcagctgaag 1560

gagtcaggac ctggcctggt ggcgccctca cagagcctgt ccatcacatg 30 caccgtctca 1620

gggttctcat taaccggcta tggtgtaaac tgggttcgcc agcctccagg aaagggtctg 1680

35 gagtggctgg gaatgatatg gggtgatgga agcacagact ataattcagc tctcaaatcc 1740

agactatcga tcaccaagga caactccaag agccaagttt tcttaaaaat gaacagtctg 1800

caaactgatg acacagccag atactactgt gctcgagatg gttatagtaa 5 ctttcattac 1860 2021201261

tatgttatgg actactgggg tcaaggaacc tcagtcaccg tctcctctgg gggtggaggc 1920

10 tctggtggcg gtggatccgg cggaggtggg tcgggtggcg gcggatctga cattgtgctc 1980

acccaatctc cagcttcttt ggctgtgtct ctaggtcaga gagccaccat ctcctgcaga 2040 15 gccagtgaaa gtgttgaata ttatgtcaca agtttaatgc agtggtacca acagaaacca 2100

ggacagccac ccaaactcct catctctgct gctagcaacg tagaatctgg 20 ggtccctgcc 2160

aggtttagtg gcagtgggtc tgggacagac tttagcctca acatccatcc tgtggaggag 2220

25 gatgatattg caatgtattt ctgtcagcaa agtaggaagg ttccatggac gttcggtgga 2280

ggcaccaagc tggaaatcaa acgttaatct aga 2313 30

<210> 225 <211> 764 <212> PRT 35 <213> Artificial sequence

<220>

<223> Synthetic polypeptide

<220> <223> n2H7sssIgG1-H5-2e12HL (AA) 5 <220> 2021201261

<221> misc_feature <222> (1)..(20) <223> Leader 10 <220> <221> misc_feature <222> (21)..(126) <223> VL 15 <220> <221> misc_feature <222> (127)..(142) <223> Linker 20 <220> <221> misc_feature <222> (143)..(264) <223> VH 25 <220> <221> misc_feature <222> (265)..(279) <223> Hinge 30 <220> <221> misc_feature <222> (497)..(511) <223> EFD-BD2 Linker 35 <220> <221> misc_feature

<222> (512)..(632) <223> VH2

<220> 5 <221> misc_feature <222> (633)..(652) 2021201261

<223> Linker2

<220> 10 <221> misc_feature <222> (653)..(764) <223> VL2

<400> 225 15 Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 1 5 10 15

20 Asp Thr Thr Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile Leu Ser 20 25 30

25 Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser 35 40 45

30 Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Lys 50 55 60

35 Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro Ala Arg

65 70 75 80

5 Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 2021201261

85 90 95

10 Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe 100 105 110

15 Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Asp Gly 115 120 125

20 Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ala Ser Gln Ala 130 135 140

25 Tyr Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala Ser Val 145 150 155 160

30 Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Asn Met 165 170 175 35

His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile

Gly Ala 180 185 190

5 Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 2021201261

195 200 205

10 Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 210 215 220

15 Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg 225 230 235 240

20 Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp Gly Thr 245 250 255 25

Gly Thr Thr Val Thr Val Ser Ser Glu Pro Lys Ser Ser Asp Lys Thr 260 265 270 30

His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 275 280 285 35

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile

Ser Arg 290 295 300

5 Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 2021201261

305 310 315 320

10 Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 325 330 335 15

Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 340 345 350 20

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 355 360 365 25

Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 370 375 380 30 Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 385 390 395 400 35

Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu

Thr Cys 405 410 415

5 Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 2021201261

Glu Ser 420 425 430

10 Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 435 440 445

15 Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 450 455 460

20 Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 465 470 475 480 25

Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490 30 495

Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Asn Ser Gln 35 500 505 510

Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 515 520 525

5 Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Gly 2021201261

Tyr Gly 530 535 540

10 Val Asn Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly 545 550 555 560 15

Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser 565 570 20 575

Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 25 580 585 590

Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr Cys Ala Arg 30 595 600 605

Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr Trp Gly Gln 35 610 615 620

Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly 625 630 635 640 5 Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile 2021201261

Val Leu 645 650 655 10

Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr 660 665 670 15

Ile Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val Thr Ser Leu 675 680 685 20

Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 690 695 700 25

Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg Phe Ser Gly 705 710 715 30 720

Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro Val Glu Glu 35 725 730

Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser Arg Lys Val Pro Trp 740 745 750 5 2021201261

Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 755 760

10 <210> 226 <211> 2322 <212> DNA <213> Artificial sequence 15 <220> <223> Synthetic polynucleotide

<220> 20 <223> n2H7sssIgG1-H6-2e12HL (DNA)

<400> 226 aagcttgccg ccatggaagc accagcgcag cttctcttcc tcctgctact ctggctccca 60 25 gataccaccg gtcaaattgt tctctcccag tctccagcaa tcctgtctgc atctccaggg 120

gagaaggtca caatgacttg cagggccagc tcaagtgtaa gttacatgca 30 ctggtaccag 180

cagaagccag gatcctcccc caaaccctgg atttatgccc catccaacct ggcttctgga 240

35 gtccctgctc gcttcagtgg cagtgggtct gggacctctt actctctcac aatcagcaga 300

gtggaggctg aagatgctgc cacttattac tgccagcagt ggagttttaa cccacccacg 360

ttcggtgctg ggaccaagct ggagctgaaa gatggcggtg gctcgggcgg 5 tggtggatct 420 2021201261

ggaggaggtg gagctagcca ggcttatcta cagcagtctg gggctgagct ggtgaggcct 480

10 ggggcctcag tgaagatgtc ctgcaaggct tctggctaca catttaccag ttacaatatg 540

cactgggtaa agcagacacc tagacagggc ctggaatgga ttggagctat ttatccagga 600 15 aatggtgata cttcctacaa tcagaagttc aagggcaagg ccacactgac tgtagacaaa 660

tcctccagca cagcctacat gcagctcagc agcctgacat ctgaagactc 20 tgcggtctat 720

ttctgtgcaa gagtggtgta ctatagtaac tcttactggt acttcgatgt ctggggcaca 780

25 gggaccacgg tcaccgtctc gagcgagccc aaatcttctg acaaaactca cacatcccca 840

ccgagcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc 900 30 aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc 960

cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt 35 gcataatgcc 1020

aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag

cgtcctcacc 1080

gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc 1140 5 ctcccagccc ccatcgagaa aacaatctcc aaagccaaag ggcagccccg 2021201261

agaaccacag 1200

gtgtacaccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag 10 cctgacctgc 1260

ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg 1320

15 gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac 1380

agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg 1440 20 atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaag 1500

ggtggcggtg gctcgggcgg tggtggatct gggggaggag gcagcgggaa 25 ttctcaggtg 1560

cagctgaagg agtcaggacc tggcctggtg gcgccctcac agagcctgtc catcacatgc 1620

30 accgtctcag ggttctcatt aaccggctat ggtgtaaact gggttcgcca gcctccagga 1680

aagggtctgg agtggctggg aatgatatgg ggtgatggaa gcacagacta taattcagct 1740 35 ctcaaatcca gactatcgat caccaaggac aactccaaga gccaagtttt cttaaaaatg 1800

aacagtctgc aaactgatga cacagccaga tactactgtg ctcgagatgg ttatagtaac 1860

5 tttcattact atgttatgga ctactggggt caaggaacct cagtcaccgt ctcctctggg 1920 2021201261

ggtggaggct ctggtggcgg tggatccggc ggaggtgggt cgggtggcgg cggatctgac 1980 10 attgtgctca cccaatctcc agcttctttg gctgtgtctc taggtcagag agccaccatc 2040

tcctgcagag ccagtgaaag tgttgaatat tatgtcacaa gtttaatgca 15 gtggtaccaa 2100

cagaaaccag gacagccacc caaactcctc atctctgctg ctagcaacgt agaatctggg 2160

20 gtccctgcca ggtttagtgg cagtgggtct gggacagact ttagcctcaa catccatcct 2220

gtggaggagg atgatattgc aatgtatttc tgtcagcaaa gtaggaaggt tccatggacg 2280 25 ttcggtggag gcaccaagct ggaaatcaaa cgttaatcta ga 2322

30 <210> 227 <211> 767 <212> PRT <213> Artificial sequence

35 <220> <223> Synthetic polypeptide

<220> <223> n2H7sssIgG1-H6-2e12HL (AA)

<220> 5 <221> misc_feature <222> (1)..(20) 2021201261

<223> Leader

<220> 10 <221> misc_feature <222> (21)..(126) <223> VL

<220> 15 <221> misc_feature <222> (127)..(142) <223> Linker

<220> 20 <221> misc_feature <222> (143)..(264) <223> VH

<220> 25 <221> misc_feature <222> (265)..(279) <223> Hinge

<220> 30 <221> misc_feature <222> (497)..(514) <223> EFD-BD2 Linker

<220> 35 <221> misc_feature <222> (515)..(635) <223> VH2

<220> <221> misc_feature <222> (636)..(655) 5 <223> Linker2 2021201261

<220> <221> misc_feature <222> (656)..(767) 10 <223> VL2

<400> 227

Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp 15 Leu Pro 1 5 10 15

Asp Thr Thr Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 Leu Ser 20 25 30

Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 25 Ser Ser 35 40 45

Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 30 Pro Lys 50 55 60

Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 35 Ala Arg 65 70 75

Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg 5 85 90 95 2021201261

Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe 10 100 105 110

Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Asp Gly 15 115 120 125

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ala Ser Gln Ala 20 130 135 140

Tyr Leu Gln Gln Ser Gly Ala Glu Leu Val Arg Pro Gly Ala Ser Val 25 145 150 155 160

Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 30 Asn Met 165 170 175

35 His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile Gly Ala 180 185 190

Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly 5 195 200 205 2021201261

Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 10 210 215 220

Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala Arg 15 225 230 235 240

Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp Gly Thr 20 245 250 255

Gly Thr Thr Val Thr Val Ser Ser Glu Pro Lys Ser Ser Asp 25 Lys Thr 260 265 270

His Thr Ser Pro Pro Ser Pro Ala Pro Glu Leu Leu Gly Gly 30 Pro Ser 275 280 285

Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 35 Ser Arg 290 295 300

Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 305 310 315 5 320 2021201261

Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 10 325 330 335

Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 15 Val Val 340 345 350

Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 20 Glu Tyr 355 360 365

Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 25 Lys Thr 370 375 380

Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 30 Thr Leu 385 390 395 400

35 Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 405 410

Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 5 Glu Ser 420 425 430 2021201261

Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 10 Leu Asp 435 440 445

Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 15 Lys Ser 450 455 460

Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 20 Glu Ala 465 470 475 480

25 Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 485 490 495

30 Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 500 505 510

35 Asn Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro 515 520 525

Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr 5 530 535 540 2021201261

Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu 10 545 550 555 560

Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr Asp Tyr Asn 15 Ser Ala 565 570 575

20 Leu Lys Ser Arg Leu Ser Ile Thr Lys Asp Asn Ser Lys Ser Gln Val 580 585 590

25 Phe Leu Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Arg Tyr Tyr 595 600 605

30 Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr Tyr Val Met Asp Tyr 610 615 620

35 Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser 625 630 635

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 5 Ser Asp 645 650 2021201261

655

10 Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly Gln 660 665 670

15 Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Glu Tyr Tyr Val 675 680 685

20 Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys 690 695 700

25 Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser Gly Val Pro Ala Arg 705 710 715 720

30 Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Ser Leu Asn Ile His Pro 725 730 735 35

Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys Gln Gln Ser

Arg Lys 740 745 750

Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 5 Arg 755 760 765 2021201261

<210> 228 10 <211> 2337 <212> DNA <213> Artificial sequence

<220> 15 <223> Synthetic polynucleotide

<220> <223> 2H7sssIgG1-H7-G281 HL (DNA)

20 <400> 228 aagcttgccg ccatggattt tcaagtgcag attttcagct tcctgctaat cagtgcttca 60

gtcataatgt ccagaggaca aattgttctc tcccagtctc cagcaatcct 25 gtctgcatct 120

ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta catgcactgg 180

30 taccagcaga agccaggatc ctcccccaaa ccctggattt atgccccatc caacctggct 240

tctggagtcc ctgctcgctt cagtggcagt gggtctggga cctcttactc tctcacaatc 300 35 agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggag ttttaaccca 360

cccacgttcg gtgctgggac caagctggag ctgaaagatg gcggtggctc gggcggtggt 420

5 ggatctggag gaggtgggag ctctcaggct tatctacagc agtctggggc tgagtcggtg 480 2021201261

aggcctgggg cctcagtgaa gatgtcctgc aaggcttctg gctacacatt taccagttac 540 10 aatatgcact gggtaaagca gacacctaga cagggcctgg aatggattgg agctatttat 600

ccaggaaatg gtgatacttc ctacaatcag aagttcaagg gcaaggccac 15 actgactgta 660

gacaaatcct ccagcacagc ctacatgcag ctcagcagcc tgacatctga agactctgcg 720

20 gtctatttct gtgcaagagt ggtgtactat agtaactctt actggtactt cgatgtctgg 780

ggcacaggga ccacggtcac cgtctctgat caggagccca aatcttgtga caaaactcac 840 25 acatccccac cgtgctcagc acctgaactc ctggggggac cgtcagtctt cctcttcccc 900

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg 30 cgtggtggtg 960

gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg 1020

35 cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc 1080

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc 1140

aacaaagccc tcccagcccc catcgagaaa acaatctcca aagccaaagg 5 gcagccccga 1200 2021201261

gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc 1260

10 ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat 1320

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc 1380 15 ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca 1440

tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct 20 ctccctgtct 1500

ccgggtaaga attatggtgg cggtggctcg ggcggtggtg gatctggagg aggtgggagt 1560

25 gggaattctc aggtgcagct gaaggagtca ggacctggcc tggtggcgcc ctcacagagc 1620

ctgtccatca catgcaccgt ctcagggttc tcattaaccg gctatggtgt aaactgggtt 1680 30 cgccagcctc caggaaaggg tctggagtgg ctgggaatga tatggggtga tggaagcaca 1740

gactataatt cagctctcaa atccagacta tcgatcacca aggacaactc 35 caagagccaa 1800

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccagatacta

ctgtgctcga 1860

gatggttata gtaactttca ttactatgtt atggactact ggggtcaagg aacctcagtc 1920 5 accgtctcct ctgggggtgg aggctctggt ggcggtggat ccggcggagg 2021201261

tgggtcgggt 1980

ggcggcggat ctgacattgt gctcacccaa tctccagctt ctttggctgt 10 gtctctaggt 2040

cagagagcca ccatctcctg cagagccagt gaaagtgttg aatattatgt cacaagttta 2100

15 atgcagtggt accaacagaa accaggacag ccacccaaac tcctcatctc tgctgctagc 2160

aacgtagaat ctggggtccc tgccaggttt agtggcagtg ggtctgggac agactttagc 2220 20 ctcaacatcc atcctgtgga ggaggatgat attgcaatgt atttctgtca gcaaagtagg 2280

aaggttccat ggacgttcgg tggaggcacc aagctggaaa tcaaacgtta 25 atctaga 2337

<210> 229 <211> 772 30 <212> PRT <213> Artificial sequence

<220> <223> Synthetic polypeptide 35 <220> <223> 2H7cscIgG1-STD1-2e12HL (w/2E12 leader) (AA)

<220> <221> misc_feature <222> (1)..(22) 5 <223> Leader 2021201261

<220> <221> misc_feature <222> (23)..(128) 10 <223> VL

<220> <221> misc_feature <222> (129)..(144) 15 <223> Linker

<220> <221> misc_feature <222> (145)..(265) 20 <223> VH

<220> <221> misc_feature <222> (268)..(282) 25 <223> Hinge

<220> <221> misc_feature <222> (500)..(519) 30 <223> EFD-BD2 Linker

<220> <221> misc_feature <222> (520)..(640) 35 <223> VH2

<220>

<221> misc_feature <222> (641)..(660) <223> Linker2

5 <220> <221> misc_feature 2021201261

<222> (661)..(772) <223> VL2

10 <400> 229

Met Asp Phe Gln Val Gln Ile Phe Ser Phe Leu Leu Ile Ser Ala Ser 1 5 10 15 15

Val Ile Met Ser Arg Gly Gln Ile Val Leu Ser Gln Ser Pro Ala Ile 20 25 30 20

Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 35 40 45 25

Ser Ser Val Ser Tyr Met His Trp Tyr Gln Gln Lys Pro Gly Ser Ser 50 55 60 30

Pro Lys Pro Trp Ile Tyr Ala Pro Ser Asn Leu Ala Ser Gly Val Pro 65 70 75 35 80

Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile 85 90 95

5 Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 2021201261

Gln Trp 100 105 110

10 Ser Phe Asn Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 115 120 125

15 Asp Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ser 130 135 140

20 Gln Ala Tyr Leu Gln Gln Ser Gly Ala Glu Ser Val Arg Pro Gly Ala 145 150 155 160 25

Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 165 170 30 175

Asn Met His Trp Val Lys Gln Thr Pro Arg Gln Gly Leu Glu Trp Ile 35 180 185 190

Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 195 200 205

5 Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr 2021201261

Ala Tyr 210 215 220

10 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 225 230 235 240 15

Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 245 250 20 255

Gly Thr Gly Thr Thr Val Thr Val Ser Asp Gln Glu Pro Lys Ser Cys 25 260 265 270

Asp Lys Thr His Thr Ser Pro Pro Cys Ser Ala Pro Glu Leu Leu Gly 30 275 280 285

Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 35 290 295 300

Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 305 310 315 320 5 2021201261

Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 325 330 10 335

His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 15 340 345 350

Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 355 360 365 20

Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 370 375 380 25

Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 385 390 395 30 400

Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 35 405 410

Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 420 425 430 5 2021201261

Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 435 440 445 10

Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 450 455 460 15

Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 465 470 475 20 480

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 25 485 490 495

Pro Gly Lys Asn Tyr Gly Gly Gly Gly Ser Gly Gly Gly Gly 30 Ser Gly 500 505 510

Gly Gly Gly Ser Gly Asn Ser Gln Val Gln Leu Lys Glu Ser 35 Gly Pro 515 520 525

Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser 530 535 540 5 2021201261

Gly Phe Ser Leu Thr Gly Tyr Gly Val Asn Trp Val Arg Gln Pro Pro 545 550 555 10 560

Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Asp Gly Ser Thr 15 565 570 575

Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Ser Ile Thr Lys 20 Asp Asn 580 585 590

Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln Thr 25 Asp Asp 595 600 605

Thr Ala Arg Tyr Tyr Cys Ala Arg Asp Gly Tyr Ser Asn Phe His Tyr 30 610 615 620

Tyr Val Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 35 625 630 635

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 645 650 5 655 2021201261

Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser Pro Ala Ser Leu Ala 10 660 665 670

Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser 15 675 680 685

Val Glu Tyr Tyr Val Thr Ser Leu Met Gln Trp Tyr Gln Gln Lys Pro 20 690 695 700

Gly Gln Pro Pro Lys Leu Leu Ile Ser Ala Ala Ser Asn Val Glu Ser 25 705 710 715 720

Gly Val Pro Ala Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 30 Phe Ser 725 730 735

35 Leu Asn Ile His Pro Val Glu Glu Asp Asp Ile Ala Met Tyr Phe Cys 740 745 750

Gln Gln Ser Arg Lys Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu 5 755 760 765 2021201261

Glu Ile Lys Arg 770 10

<210> 230 <211> 45 <212> DNA 15 <213> Artificial sequence

<220> <223> Synthetic polynucleotide

20 <220> <223> scs(s) -hIgG1 (DNA)

<400> 230 gagcccaaat cttctgacaa aactcacaca tctccaccga gctca 25 45

<210> 231 <211> 15 30 <212> PRT <213> Artificial sequence

<220> <223> Synthetic polypeptide 35 <220> <223> scs(s) -hIgG1 (AA)

<400> 231

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Ser Pro Pro Ser 5 Ser 1 5 10 15 2021201261

<210> 232 10 <211> 45 <212> DNA <213> Artificial sequence

<220> 15 <223> Synthetic polynucleotide

<220> <223> ccc(s) (DNA)

20 <400> 232 gagcccaaat cttgtgacaa aactcacaca tctccaccgt gctca 45

25 <210> 233 <211> 15 <212> PRT <213> Artificial sequence

30 <220> <223> Synthetic polypeptide

<220> <223> ccc(s) (AA) 35 <400> 233

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Ser Pro Pro Cys Ser 1 5 10 15

5 <210> 234 2021201261

<211> 24 <212> DNA <213> Artificial sequence 10 <220> <223> Synthetic polynucleotide

15 <220> <223> Linker H8 (PN)

<400> 234 gggtctccac cttctccgaa ttct 20 24

<210> 235 <211> 8 25 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 30 <400> 235

Gly Ser Pro Pro Ser Pro Asn Ser 1 5 35

<210> 236

<211> 21 <212> DNA <213> Artificial sequence

5 <220> <223> Synthetic polynucleotide 2021201261

<220> 10 <223> Linker H9 (PN)

<400> 236 tctccacctt ctccgaattc t 21 15

<210> 237 <211> 7 <212> PRT 20 <213> Artificial sequence

<220> <223> Synthetic peptide

25 <220> <223> Linker H9 (AA)

<400> 237 30 Ser Pro Pro Ser Pro Asn Ser 1 5

35 <210> 238

<400> 238

<210> 239

5 <400> 239 000 2021201261

<210> 240 <211> 51 10 <212> DNA <213> Artificial sequence

<220> <223> Synthetic polynucleotide 15 <220> <223> Linker H11 (PN)

<400> 240 20 gagcccacat ctaccgacaa aactcacaca tctccaccca gcccgaattc t 51

<210> 241 25 <211> 17 <212> PRT <213> Artificial sequence

<220> 30 <223> Synthetic polynucleotide

<220> <223> Linker H11 (AA)

35 <400> 241

Glu Pro Thr Ser Thr Asp Lys Thr His Thr Ser Pro Pro Ser

Pro Asn 1 5 10 15

5 Ser 2021201261

<210> 242 <211> 51 10 <212> DNA <213> Artificial sequence

<220> <223> Synthetic polynucleotide 15 <220> <223> Linker H12 (PN)

<400> 242 20 gagcccacat ctaccgacaa aactcacaca tctccaccca gcccgaattc t 51

<210> 243 25 <211> 17 <212> PRT <213> Artificial sequence

<220> 30 <223> Synthetic peptide

<220> <223> Linker H12 (AA)

35 <400> 243

Glu Pro Thr Ser Thr Asp Lys Thr His Thr Cys Pro Pro Cys

Pro Asn 1 5 10 15

5 Ser 2021201261

<210> 244 <211> 51 <212> DNA 10 <213> Artificial sequence

<220> <223> Synthetic polynucleotide

15 <220> <223> Linker H13 (PN)

<400> 244 gagcccacat ctaccgacaa aactcacaca tctccaccca gcccgaattc t 20 51

<210> 245 <211> 17 25 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 30 <220> <223> Linker H13 (AA)

<400> 245 35 Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Asn

1 5 10 15

Ser 5 <210> 246 2021201261

<211> 36 <212> DNA <213> Artificial sequence 10 <220> <223> Synthetic polynucleotide

<220> 15 <223> Linker H15 (PN)

<400> 246 ggcggtggtg gctcctgtcc accttgtccg aattct 36 20

<210> 247 <211> 12 <212> PRT 25 <213> Artificial sequence

<220> <223> Synthetic peptide

30 <220> <223> Linker H15 (AA)

<400> 247 35 Gly Gly Gly Gly Ser Cys Pro Pro Cys Pro Asn Ser 1 5 10

<210> 248 <211> 45 5 <212> DNA <213> Artificial sequence 2021201261

<220> <223> Synthetic polynucleotide 10 <220> <223> Linker H16 (PN)

<400> 248 15 ctgtctgtga aagctgactt cctcactcca tccatcggga attct 45

<210> 249 20 <211> 15 <212> PRT <213> Artificial sequence

<220> 25 <223> Synthetic peptide

<220> <223> Linker H16 (AA) 30 <400> 249

Leu Ser Val Lys Ala Asp Phe Leu Thr Pro Ser Ile Gly Asn Ser 35 1 5 10 15

<210> 250 <211> 60 <212> DNA <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic polynucleotide

10 <220> <223> Linker H17 (PN)

<400> 250 ctgtctgtga aagctgactt cctcactcca tccatctcct gtccaccttg 15 cccgaattct 60

<210> 251 <211> 20 20 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 25

<220> <223> Linker H17 (AA)

30 <400> 251

Leu Ser Val Lys Ala Asp Phe Leu Thr Pro Ser Ile Ser Cys Pro Pro 1 5 10 15 35

Cys Pro Asn Ser

<210> 252 5 <211> 45 <212> DNA 2021201261

<213> Artificial sequence

<220> 10 <223> Synthetic polynucleotide

<220> <223> Linker H18 (PN) 15 <400> 252 ctgtctgtgc tcgctaactt cagtcagcca gagatcggga attct 45

20 <210> 253 <211> 15 <212> PRT <213> Artificial sequence 25 <220> <223> Synthetic peptide

30 <220> <223> Linker H18 (AA)

<400> 253

35 Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Gly Asn Ser 1 5 10 15

<210> 254 <211> 60 5 <212> DNA <213> Artificial sequence 2021201261

<220> <223> Synthetic polynucleotide 10

<220> <223> Linker H19 (PN)

15 <400> 254 ctgtctgtgc tcgctaactt cagtcagcca gagatctcct gtccaccttg cccgaattct 60

20 <210> 255 <211> 20 <212> PRT <213> Artificial sequence

25 <220> <223> Synthetic peptide

<220> 30 <223> Linker H19 (AA)

<400> 255

Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Ser Cys 35 Pro Pro 1 5 10 15

Cys Pro Asn Ser 20

5 <210> 256 2021201261

<211> 45 <212> DNA <213> Artificial sequence 10 <220> <223> Synthetic polynucleotide

15 <220> <223> Linker H20 (PN)

<400> 256 ctgaaaatcc aggagagggt cagtaagcca aagatctcga attct 20 45

<210> 257 <211> 15 25 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 30

<220> <223> Linker H20 (AA)

35 <400> 257

Leu Lys Ile Gln Glu Arg Val Ser Lys Pro Lys Ile Ser Asn

Ser 1 5 10 15

5 <210> 258 <211> 60 2021201261

<212> DNA <213> Artificial sequence

10 <220> <223> Synthetic polypeptide

<220> 15 <223> Linker H21 (PN)

<400> 258 ctgaaaatcc aggagagggt cagtaagcca aagatctcct gtccaccttg cccgaattct 60 20

<210> 259 <211> 20 <212> PRT 25 <213> Artificial sequence

<220> <223> Synthetic peptide

30 <220> <223> Linker H21 (AA)

<400> 259

35 Leu Lys Ile Gln Glu Arg Val Ser Lys Pro Lys Ile Ser Cys Pro Pro 1 5 10 15

Cys Pro Asn Ser 20 5 2021201261

<210> 260 <211> 45 <212> DNA 10 <213> Artificial sequence

<220> <223> Synthetic polypeptide

15 <220> <223> Linker H22 (PN)

<400> 260 ctggatgtga gtgagaggcc ttttcctcca cacatccaga attct 20 45

<210> 261 <211> 15 25 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 30

<220> <223> Linker H22 (AA)

35 <400> 261

Leu Asp Val Ser Glu Arg Pro Phe Pro Pro His Ile Gln Asn

Ser 1 5 10 15

5 <210> 262 <211> 63 2021201261

<212> DNA <213> Artificial sequence

10 <220> <223> Synthetic polynucleotide

<220> 15 <223> Linker H23 (PN)

<400> 262 ctggatgtga gtgagaggcc ttttcctcca cacatccagt cctgtccacc ttgcccgaat 60 20 tct 63

25 <210> 263 <211> 21 <212> PRT <213> Artificial sequence

30 <220> <223> Synthetic peptide

<220> <223> Linker H23 (AA) 35 <400> 263

Leu Asp Val Ser Glu Arg Pro Phe Pro Pro His Ile Gln Ser Cys Pro 1 5 10 15

5 Pro Cys Pro Asn Ser 2021201261

20

10 <210> 264 <211> 45 <212> DNA <213> Artificial sequence

15 <220> <223> Synthetic polynucleotide

<220> <223> Linker H24 (DNA) 20 <400> 264 cgggaacagc tggcagaggt cactttgagc ttgaaagcga attct 45

25 <210> 265 <211> 15 <212> PRT <213> Artificial sequence 30 <220> <223> Synthetic peptide

<220> 35 <223> Linker H24 (AA)

<400> 265

Arg Glu Gln Leu Ala Glu Val Thr Leu Ser Leu Lys Ala Asn Ser 1 5 10 15 5 2021201261

<210> 266 <211> 60 <212> DNA 10 <213> Artificial sequence

<220> <223> Synthetic polynucleotide

15 <220> <223> Linker H25 (PN)

<400> 266 20 cgggaacagc tggcagaggt cactttgagc gtgaaagctt gtccaccctg cccgaattct 60

<210> 267 25 <211> 20 <212> PRT <213> Artificial sequence

<220> 30 <223> Synthetic peptide

<220> <223> Linker H25 (AA) 35 <400> 267

Arg Glu Gln Leu Ala Glu Val Thr Leu Ser Leu Lys Ala Cys Pro Pro 1 5 10 15

5 Cys Pro Asn Ser 2021201261

20

10 <210> 268 <211> 45 <212> DNA <213> Artificial sequence

15 <220> <223> Synthetic polynucleotide

<220> 20 <223> Linker H26 (PN)

<400> 268 cggattcacc agatgaactc cgagttgagc gtgctcgcga attct 45 25

<210> 269 <211> 15 <212> PRT 30 <213> Artificial sequence

<220> <223> Synthetic peptide

35 <220> <223> Linker H26 (AA)

<400> 269

Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala Asn 5 Ser 1 5 10 15 2021201261

<210> 270 10 <211> 60 <212> DNA <213> Artificial sequence

<220> 15 <223> Synthetic polynucleotide

<220> <223> Linker H27 (PN) 20 <400> 270 cggattcacc agatgaactc cgagttgagc gtgctcgctt gtccaccctg cccgaattct 60

25 <210> 271 <211> 20 <212> PRT <213> Artificial sequence 30 <220> <223> Synthetic peptide

<220> 35 <223> Linker H27 (AA)

<400> 271

Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala Cys Pro Pro 1 5 10 15 5 2021201261

Cys Pro Asn Ser 20

10 <210> 272 <211> 45 <212> DNA <213> Artificial sequence 15 <220> <223> Synthetic polynucleotide

20 <220> <223> Linker H28 (PN)

<400> 272 gataccaaag ggaagaacgt cctcgagaag atcttctcga attct 25 45

<210> 273 <211> 15 30 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 35

<220>

<223> Linker H28 (AA)

<400> 273

5 Asp Thr Lys Gly Lys Asn Val Leu Glu Lys Ile Phe Ser Asn Ser 2021201261

1 5 10 15

10 <210> 274 <211> 63 <212> DNA <213> Artificial sequence

15 <220> <223> Synthetic polynucleotide

<220> 20 <223> Linker H29 (PN)

<400> 274 gataccaaag ggaagaacgt cctcgagaag atcttcgact cctgtccacc ttgcccgaat 60 25 tct 63

30 <210> 275 <211> 21 <212> PRT <213> Artificial sequence

35 <220> <223> Synthetic peptide

<220> <223> Linker H29 (AA)

5 <400> 275 2021201261

Asp Thr Lys Gly Lys Asn Val Leu Glu Lys Ile Phe Asp Ser Cys Pro 1 5 10 15 10

Pro Cys Pro Asn Ser 20

15 <210> 276 <211> 45 <212> DNA <213> Artificial sequence 20 <220> <223> Synthetic polynucleotide

25 <220> <223> Linker H30 (PN)

<400> 276 ctgccacctg agacacagga gagtcaagaa gtcaccctga attct 30 45

<210> 277 <211> 15 35 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide

5 <220> <223> Linker H30 (AA) 2021201261

<400> 277

10 Leu Pro Pro Glu Thr Gln Glu Ser Gln Glu Val Thr Leu Asn Ser 1 5 10 15

15 <210> 278 <211> 63 <212> DNA <213> Artificial sequence

20 <220> <223> Synthetic polynucleotide

<220> 25 <223> Linker H31 (PN)

<400> 278 ctgccacctg agacacagga gagtcaagaa gtcaccctgt cctgtccacc ttgcccgaat 60 30 tct 63

35 <210> 279 <211> 21 <212> PRT

<213> Artificial sequence

<220> <223> Synthetic peptide 5 2021201261

<220> <223> Linker H31 (AA)

10 <400> 279

Leu Pro Pro Glu Thr Gln Glu Ser Gln Glu Val Thr Leu Ser Cys Pro 1 5 10 15 15

Pro Cys Pro Asn Ser 20

20 <210> 280 <211> 45 <212> DNA <213> Artificial sequence 25 <220> <223> Synthetic polynucleotide

30 <220> <223> Linker H32 (PN)

<400> 280 cggattcacc tgaacgtgtc cgagaggccc tttcctccga attct 35 45

<210> 281 <211> 15 <212> PRT <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic peptide

10 <220> <223> Linker H32 (AA)

<400> 281

15 Arg Ile His Leu Asn Val Ser Glu Arg Pro Phe Pro Pro Asn Ser 1 5 10 15

20 <210> 282 <211> 60 <212> DNA <213> Artificial sequence

25 <220> <223> Synthetic polynucleotide

<220> 30 <223> Linker H33 (PN)

<400> 282 cggattcacc tgaacgtgtc cgagaggccc tttcctccct gtccaccctg cccgaattct 60 35

<210> 283

<211> 20 <212> PRT <213> Artificial sequence

5 <220> <223> Synthetic peptide 2021201261

<220> 10 <223> Linker H33 (AA)

<400> 283

Arg Ile His Leu Asn Val Ser Glu Arg Pro Phe Pro Pro Cys 15 Pro Pro 1 5 10 15

Cys Pro Asn Ser 20 20

<210> 284

25 <400> 284 000

<210> 285

30 <400> 285 000

<210> 286

35 <400> 286

<210> 287

<400> 287 000 5 <210> 288 2021201261

<211> 39 <212> DNA <213> Artificial sequence 10 <220> <223> Synthetic peptide

15 <220> <223> Linker H36 (PN)

<400> 288 gggtgtccac cttgtccagg cggtggtgga tcgaattct 20 39

<210> 289 <211> 13 25 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 30

<220> <223> Linker H36 (AA)

35 <400> 289

Gly Cys Pro Pro Cys Pro Gly Gly Gly Gly Ser Asn Ser

1 5 10

<210> 290 5 <400> 290 2021201261

000

<210> 291 10 <400> 291 000

<210> 292 15 <400> 292 000

<210> 293 20 <400> 293 000

<210> 294 25 <400> 294 000

<210> 295 30 <400> 295 000

<210> 296 35 <211> 27 <212> DNA <213> Artificial sequence

<220> <223> Synthetic polynucleotide

5 <220> 2021201261

<223> Linker H40 (PN)

<400> 296 10 ggatgtccac cttgtcccgc gaattct 27

<210> 297 15 <211> 9 <212> PRT <213> Artificial sequence

<220> 20 <223> Synthetic peptide

<220> <223> Linker H40 (AA) 25 <400> 297

Gly Cys Pro Pro Cys Pro Ala Asn Ser 1 5 30

<210> 298

<400> 298 35 000

<210> 299

<211> 9 <212> PRT <213> Artificial sequence

5 <220> <223> Synthetic peptide 2021201261

<220> 10 <221> misc_feature <223> Linker H41

<400> 299

15 Gly Cys Pro Pro Cys Pro Ala Asn Ser 1 5

<210> 300 20 <400> 300 000

<210> 301 25 <211> 8 <212> PRT <213> Artificial sequence

<220> 30 <223> Synthetic peptide

<220> <223> Linker H42(AA)

35 <400> 301

Gly Cys Pro Pro Cys Pro Asn Ser

1 5

<210> 302 5 <400> 302 2021201261

000

<210> 303 10 <400> 303 000

<210> 304 15 <211> 36 <212> DNA <213> Artificial sequence

<220> 20 <223> Synthetic polynucleotide

<220> <223> Linker H44 (PN) 25 <400> 304 ggaggagcta gttgtccacc ttgtcccggg aattct 36

30 <210> 305 <211> 12 <212> PRT <213> Artificial sequence 35 <220> <223> Synthetic peptide

<220> <223> Linker H44 (AA) 5 <400> 305 2021201261

Gly Gly Ala Ser Cys Pro Pro Cys Pro Gly Asn Ser 1 5 10 10

<210> 306 <211> 36 <212> DNA 15 <213> Artificial sequence

<220> <223> Synthetic polynucleotide

20 <220> <223> Linker H45 (PN)

<400> 306 25 ggaggagcca gttgtccacc ttgtgccggg aattct 36

<210> 307 30 <211> 12 <212> PRT <213> Artificial sequence

<220> 35 <223> Synthetic peptide

<220> <223> Linker H45 (AA)

<400> 307 5 Gly Gly Ala Ser Cys Pro Pro Cys Ala Gly Asn Ser 2021201261

1 5 10

10 <210> 308 <211> 33 <212> DNA <213> Artificial sequence

15 <220> <223> Synthetic polynucleotide

<220> <223> Linker H46 20 <400> 308 ggaggagcca gttgtccacc ttgtgcgaat tct 33

25 <210> 309 <211> 11 <212> PRT <213> Artificial sequence

30 <220> <223> Synthetic peptide

<220> <223> Linker H46 35 <400> 309 Gly Gly Ala Ser Cys Pro Pro Cys Ala Asn Ser

1 5 10

<210> 310 <211> 15 5 <212> PRT <213> Artificial sequence 2021201261

<220> <223> Synthetic peptide 10 <220> <223> Linker H47

<400> 310 15 Leu Ser Val Lys Ala Asp Phe Leu Thr Pro Ser Ile Gly Asn Ser 1 5 10 15

20 <210> 311 <211> 12 <212> PRT <213> Artificial sequence 25 <220> <223> Synthetic peptide

<220> 30 <223> Linker H48

<400> 311

Lys Ala Asp Phe Leu Thr Pro Ser Ile Gly Asn Ser 35 1 5 10

<210> 312

<400> 312 000 5 <210> 313 2021201261

<211> 15 <212> PRT <213> Artificial sequence 10 <220> <223> Synthetic peptide

<220> 15 <223> Linker H50

<400> 313

Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Gly Asn 20 Ser 1 5 10 15

<210> 314 25 <211> 15 <212> PRT <213> Artificial sequence

<220> 30 <223> Synthetic peptide

<220> <223> Linker H51 35 <400> 314

Leu Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Gly Asn Ser 1 5 10 15

5 <210> 315 2021201261

<211> 11 <212> PRT <213> Artificial sequence 10 <220> <223> Synthetic peptide

<220> 15 <223> Linker H52

<400> 315

Ser Gln Pro Glu Ile Val Pro Ile Ser Asn Ser 20 1 5 10

<210> 316 <211> 16 25 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 30

<220> <223> Linker H53

35 <400> 316

Ser Gln Pro Glu Ile Val Pro Ile Ser Cys Pro Pro Cys Pro

Asn Ser 1 5 10 15

5 <210> 317 <211> 19 2021201261

<212> PRT <213> Artificial sequence

10 <220> <223> Synthetic peptide

<220> 15 <223> Linker H54

<400> 317

Ser Val Leu Ala Asn Phe Ser Gln Pro Glu Ile Ser Cys Pro 20 Pro Cys 1 5 10 15

Pro Asn Ser 25

<210> 318 <211> 15 30 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 35

<220>

<223> Linker H55

<400> 318

5 Arg Ile His Gln Met Asn Ser Glu Leu Ser Val Leu Ala Asn Ser 2021201261

1 5 10 15

10 <210> 319 <211> 12 <212> PRT <213> Artificial sequence

15 <220> <223> Synthetic peptide

<220> <223> Linker H56 20 <400> 319

Gln Met Asn Ser Glu Leu Ser Val Leu Ala Asn Ser 1 5 10 25

<210> 320 <211> 10 <212> PRT 30 <213> Artificial sequence

<220> <223> Synthetic peptide

35 <220> <223> Linker H57

<400> 320

Val Ser Glu Arg Pro Phe Pro Pro Asn Ser 5 1 5 10 2021201261

<210> 321 <211> 15 10 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 15 <220> <223> Linker H58

<400> 321 20 Lys Pro Phe Phe Thr Cys Gly Ser Ala Asp Thr Cys Pro Asn Ser 1 5 10 15

25 <210> 322 <211> 15 <212> PRT <213> Artificial sequence 30 <220> <223> Synthetic peptide

<220> 35 <223> Linker H59

<400> 322

Lys Pro Phe Phe Thr Cys Gly Ser Ala Asp Thr Cys Pro Asn Ser 1 5 10 15 5 <210> 323 2021201261

<211> 14 <212> PRT <213> Artificial sequence 10 <220> <223> Synthetic peptide

15 <220> <223> Linker H60

<400> 323

20 Gln Tyr Asn Cys Pro Gly Gln Tyr Thr Phe Ser Met Asn Ser 1 5 10

<210> 324 25 <211> 21 <212> PRT <213> Artificial sequence

<220> 30 <223> Synthetic peptide

<220> <223> Linker H61 35 <400> 324

Glu Pro Ala Phe Thr Pro Gly Pro Asn Ile Glu Leu Gln Lys Asp Ser 1 5 10 15

5 Asp Cys Pro Asn Ser 2021201261

20

10 <210> 325 <211> 21 <212> PRT <213> Artificial sequence

15 <220> <223> Synthetic peptide

<220> 20 <223> Linker H62

<400> 325

Gln Arg His Asn Asn Ser Ser Leu Asn Thr Arg Thr Gln Lys 25 Ala Arg 1 5 10 15

His Cys Pro Asn Ser 30 20

<210> 326 <211> 19 35 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide

5 <220> <223> Linker H63 2021201261

<400> 326

10 Asn Ser Leu Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr 1 5 10 15

15 Cys Asn Ser

<210> 327 20 <211> 21 <212> PRT <213> Artificial sequence

<220> 25 <223> Synthetic peptide

<400> 327

Gln Arg His Asn Asn Ser Ser Leu Asn Thr Arg Thr Gln Lys 30 Ala Arg 1 5 10 15

His Cys Pro Asn Ser 35 20

<210> 328 <211> 21 <212> PRT <213> Artificial sequence 5 <220> 2021201261

<223> Synthetic peptide

10 <220> <223> Linker H65

<400> 328

15 Glu Pro Ala Phe Thr Pro Gly Pro Asn Ile Glu Leu Gln Lys Asp Ser 1 5 10 15

20 Asp Cys Pro Asn Ser 20

<210> 329 25 <211> 21 <212> PRT <213> Artificial sequence

<220> 30 <223> Synthetic peptide

<220> <223> Linker H66

35 <400> 329

Glu Pro Ala Phe Thr Pro Gly Pro Asn Ile Glu Leu Gln Lys

Asp Ser 1 5 10 15

5 Asp Cys Pro Asn Ser 20 2021201261

<210> 330 10 <211> 21 <212> PRT <213> Artificial sequence

<220> 15 <223> Synthetic peptide

<220> <223> Linker H67 20 <400> 330

Glu Pro Ala Phe Thr Pro Gly Pro Asn Ile Glu Leu Gln Lys Asp Ser 25 1 5 10 15

Asp Cys Pro Asn Ser 20 30

<210> 331 <211> 118 <212> PRT 35 <213> Artificial sequence

<220>

<223> Synthetic peptide

<220> <223> Linker H68 5 <400> 331 2021201261

Arg Thr Arg Tyr Leu Gln Val Ser Gln Gln Leu Gln Gln Thr Asn Arg 10 1 5 10 15

Val Leu Glu Val Thr Asn Ser Ser Leu Arg Gln Gln Leu Arg Leu Lys 15 20 25 30

Ile Thr Gln Leu Gly Gln Ser Ala Glu Asp Leu Gln Gly Ser Arg Arg 20 35 40 45

Glu Leu Ala Gln Ala Gln Glu Ala Leu Gln Val Glu Gln Arg Ala His 25 50 55 60

Gln Ala Ala Glu Gly Gln Leu Gln Ala Cys Gln Ala Asp Arg Gln Lys 30 65 70 75 80

Thr Lys Glu Thr Leu Gln Ser Glu Glu Gln Gln Arg Arg Ala 35 Leu Glu 85 90 95

Gln Lys Leu Ser Asn Met Glu Asn Arg Leu Lys Pro Phe Phe Thr Cys 100 105 110 5 2021201261

Gly Ser Ala Asp Thr Cys 115

10 <210> 332 <211> 10 <212> PRT <213> Artificial sequence 15 <220> <223> Synthetic peptide

20 <220> <223> anti-CD-20 VL CDR1

<400> 332

25 Arg Ala Ser Ser Ser Val Ser Tyr Ile His 1 5 10

<210> 333 30 <211> 10 <212> PRT <213> Artificial sequence

<220> 35 <223> Synthetic peptide

<220> <223> anti-CD-20 VL CDR1

<400> 333 5 Arg Ala Ser Ser Ser Val Ser Tyr Ile Val 2021201261

1 5 10

10 <210> 334 <211> 9 <212> PRT <213> Artificial sequence

15 <220> <223> Synthetic peptide

<220> 20 <223> anti-CD-20 VL CDR3

<400> 334

Gln Gln Trp Ser Phe Asn Pro Pro Thr 25 1 5

<210> 335 <211> 9 30 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 35

<220>

<223> anti-CD-20 VL CDR3

<400> 335

5 Gln Gln Tyr Ser Phe Asn Pro Pro Thr 1 5 2021201261

<210> 336 10 <211> 17 <212> PRT <213> Artificial sequence

<220> 15 <223> Synthetic peptide

<220> <223> anti-CD-20 VH CDR2 20 <400> 336

Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys 25 1 5 10 15

Gly

30

<210> 337 <211> 17 <212> PRT 35 <213> Artificial sequence

<220>

<223> Synthetic peptide

<220> 5 <223> anti-CD-20 VH CDR2 2021201261

<400> 337

Ala Ile Tyr Pro Gly Asn Gly Glu Thr Ser Tyr Asn Gln Lys 10 Phe Lys 1 5 10 15

Gly 15

<210> 338 <211> 12 20 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 25

<220> <223> anti-CD20 VH CDR3

30 <400> 338

Ser Val Tyr Tyr Ser Asn Tyr Trp Tyr Phe Asp Leu 1 5 10

35 <210> 339 <211> 12

<212> PRT <213> Artificial sequence

<220> 5 <223> Synthetic peptide 2021201261

<220> <223> anti-CD20 VH CDR3 10 <400> 339

Ser Val Tyr Tyr Gly Gly Tyr Trp Tyr Phe Asp Leu 1 5 10 15

<210> 340 <211> 12 <212> PRT 20 <213> Artificial sequence

<220> <223> Synthetic peptide

25 <220> <223> anti-CD20 VH CDR3

<400> 340 30 Ser Tyr Tyr Ser Asn Ser Asp Trp Tyr Phe Asp Leu 1 5 10

35 <210> 341 <211> 12 <212> PRT

<213> Artificial sequence

<220> <223> Synthetic peptide 5 2021201261

<220> <223> anti-CD20 VH CDR3

10 <400> 341

Ser Tyr Tyr Ser Gly Gly Asp Trp Tyr Phe Asp Leu 1 5 10

15 <210> 342 <211> 12 <212> PRT <213> Artificial sequence 20 <220> <223> Synthetic peptide

25 <220> <223> anti-CD20 VH CDR3

<400> 342

30 Ser Tyr Lys Ser Asn Ser Tyr Trp Tyr Phe Asp Leu 1 5 10

<210> 343 35 <211> 12 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide

5 <220> 2021201261

<223> anti-CD20 VH CDR3

<400> 343 10 Ser Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Leu 1 5 10

15 <210> 344 <211> 12 <212> PRT <213> Artificial sequence

20 <220> <223> Synthetic peptide

<220> 25 <223> anti-CD-20 VH CDR3

<400> 344

Ser Tyr Lys Ser Asn Ser Asp Trp Tyr Phe Asp Leu 30 1 5 10

<210> 345 <211> 12 35 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide

5 <220> <223> anti-CD20 VH CDR3 2021201261

<400> 345

10 Ser Tyr Lys Ser Gly Gly Asp Trp Tyr Phe Asp Leu 1 5 10

<210> 346 15 <211> 8 <212> PRT <213> Artificial sequence

<220> 20 <223> Synthetic peptide

<220> <223> Scorpion linker core (2H7) sequence 25 <400> 346

Gly Cys Pro Pro Cys Pro Asn Ser 1 5 30

<210> 347 <211> 4 <212> PRT 35 <213> Artificial sequence

<220>

<223> Synthetic peptide

<220> 5 <223> Extension sequence 2021201261

<400> 347

Ala Pro Glu Leu 10 1

<210> 348 <211> 9 15 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 20

<220> <223> Extension sequence

25 <400> 348

Ala Pro Glu Leu Gly Gly Gly Gly Ser 1 5

30 <210> 349 <211> 14 <212> PRT <213> Artificial sequence 35 <220> <223> Synthetic peptide

<220> <223> Extension sequence 5 <400> 349 2021201261

Ala Pro Glu Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 1 5 10 10

<210> 350 <211> 19 <212> PRT 15 <213> Artificial sequence

<220> <223> Synthetic peptide

20 <220> <223> Extension sequence

<400> 350 25 Ala Pro Glu Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 1 5 10 15

30 Gly Gly Ser

35 <210> 351 <211> 12 <212> PRT

<213> Artificial sequence

<220> <223> Synthetic peptide 5 2021201261

<220> <223> Extended scorpion linker

10 <400> 351

Gly Cys Pro Pro Cys Pro Asn Ser Ala Pro Glu Leu 1 5 10

15 <210> 352 <211> 17 <212> PRT <213> Artificial sequence 20 <220> <223> Extended scorpion linker

<400> 352 25 Gly Cys Pro Pro Cys Pro Asn Ser Ala Pro Glu Leu Gly Gly Gly Gly 1 5 10 15

30 Ser

35 <210> 353 <211> 22 <212> PRT

<213> Artificial sequence

<220> <223> Synthetic peptide 5 2021201261

<220> <223> Extended scorpion linker

10 <400> 353

Gly Cys Pro Pro Cys Pro Asn Ser Ala Pro Glu Leu Gly Gly Gly Gly 1 5 10 15 15

Ser Gly Gly Gly Gly Ser 20

20 <210> 354 <211> 27 <212> PRT <213> Artificial sequence 25 <220> <223> Synthetic peptide

30 <220> <223> Extended scorpion linker

<400> 354

35 Gly Cys Pro Pro Cys Pro Asn Ser Ala Pro Glu Leu Gly Gly Gly Gly 1 5 10 15

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 20 25 5 2021201261

<210> 355 <211> 51 <212> PRT 10 <213> Artificial sequence

<220> <223> Synthetic peptide

15 <220> <223> VL CDR1-X-VL CDR3-X- VH CDR2-X-VH CDR3

<220> 20 <221> misc_feature <222> (11)..(11) <223> Xaa = a range of amino acids between VL CDR1 and VL CDR3

25 <220> <221> misc_feature <222> (21)..(21) <223> Xaa = a range of amino acids between VL CDR3 and VH CDR2 30 <220> <221> misc_feature <222> (39)..(39) <223> Xaa = a range of amino acids between VH CDR2 and VH 35 CDR3

<400> 355

Arg Ala Ser Ser Ser Val Ser Tyr Ile His Xaa Gln Gln Trp Ser Phe 1 5 10 15 5 2021201261

Asn Pro Pro Thr Xaa Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr 20 25 30 10

Asn Gln Lys Phe Lys Gly Xaa Ser Val Tyr Tyr Ser Asn Tyr Trp Tyr 35 40 45 15

Phe Asp Leu 50

20 <210> 356 <211> 51 <212> PRT <213> Artificial sequence 25 <220> <223> Synthetic peptide

30 <220> <223> VLCDR1-X-VLCDR3-X- VHCDR2-X-VHCDR3

<220> <221> misc_feature 35 <222> (11)..(11) <223> Xaa = a range of amino acids between VL CDR1 and VL CDR3

<220> <221> misc_feature <222> (21)..(21) 5 <223> Xaa = a range of amino acids between VL CDR3 and VH CDR2 2021201261

<220> <221> misc_feature 10 <222> (39)..(39) <223> Xaa = a range of amino acids between VH CDR2 and VH CDR3

<400> 356 15 Arg Ala Ser Ser Ser Val Ser Tyr Ile His Xaa Gln Gln Trp Ser Phe 1 5 10 15

20 Asn Pro Pro Thr Xaa Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr 20 25 30

25 Asn Gln Lys Phe Lys Gly Xaa Ser Val Tyr Tyr Gly Gly Tyr Trp Tyr 35 40 45

30 Phe Asp Leu 50

35 <210> 357 <211> 51 <212> PRT

<213> Artificial sequence

<220> <223> Synthetic peptide 5 2021201261

<220> <223> VLCDR1-X-VLCDR3-X- VHCDR2-X-VHCDR3

10 <220> <221> misc_feature <222> (11)..(11) <223> Xaa = a range of amino acids between VL CDR1 and VL CDR3 15 <220> <221> misc_feature <222> (21)..(21) <223> Xaa = a range of amino acids between VL CDR3 and VH 20 CDR2

<220> <221> misc_feature <222> (39)..(39) 25 <223> Xaa = a range of amino acids between VH CDR2 and VH CDR3

<400> 357

30 Arg Ala Ser Ser Ser Val Ser Tyr Ile His Xaa Gln Gln Trp Ser Phe 1 5 10 15

35 Asn Pro Pro Thr Xaa Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr 20 25 30

Asn Gln Lys Phe Lys Gly Xaa Ser Tyr Tyr Ser Asn Ser Asp Trp Tyr 5 35 40 45 2021201261

Phe Asp Leu 50 10

<210> 358 <211> 51 <212> PRT 15 <213> Artificial sequence

<220> <223> Synthetic peptide

20 <220> <223> VLCDR1-X-VLCDR3-X- VHCDR2-X-VHCDR3

<220> 25 <221> misc_feature <222> (11)..(11) <223> Xaa = a range of amino acids between VL CDR1 and VL CDR3

30 <220> <221> misc_feature <222> (21)..(21) <223> Xaa = a range of amino acids between VL CDR3 and VH CDR2 35 <220> <221> misc_feature

<222> (39)..(39) <223> Xaa = a range of amino acids between VH CDR2 and VH CDR3

5 <400> 358 2021201261

Arg Ala Ser Ser Ser Val Ser Tyr Ile His Xaa Gln Gln Trp Ser Phe 1 5 10 15 10

Asn Pro Pro Thr Xaa Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr 20 25 30 15

Asn Gln Lys Phe Lys Gly Xaa Ser Tyr Tyr Ser Gly Gly Asp Trp Tyr 35 40 45 20

Phe Asp Leu 50

25 <210> 359 <211> 51 <212> PRT <213> Artificial sequence 30 <220> <223> Synthetic peptide

35 <220> <223> VLCDR1-X-VLCDR3-X- VHCDR2-X-VHCDR3

<220> <221> misc_feature <222> (11)..(11) <223> Xaa = a range of amino acids between VL CDR1 and VL 5 CDR3 2021201261

<220> <221> misc_feature <222> (21)..(21) 10 <223> Xaa = a range of amino acids between VL CDR3 and VH CDR2

<220> <221> misc_feature 15 <222> (39)..(39) <223> Xaa = a range of amino acids between VH CDR2 and VH CDR3

<400> 359 20 Arg Ala Ser Ser Ser Val Ser Tyr Ile Val Xaa Gln Gln Trp Ser Phe 1 5 10 15

25 Asn Pro Pro Thr Xaa Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr 20 25 30

30 Asn Gln Lys Phe Lys Gly Xaa Ser Tyr Lys Ser Asn Ser Tyr Trp Tyr 35 40 45

35 Phe Asp Leu

<210> 360 <211> 51 5 <212> PRT <213> Artificial sequence 2021201261

<220> <223> Synthetic peptide 10

<220> <223> VLCDR1-X-VLCDR3-X- VHCDR2-X-VHCDR3

15 <220> <221> misc_feature <222> (11)..(11) <223> Xaa = a range of amino acids between VL CDR1 and VL CDR3 20 <220> <221> misc_feature <222> (21)..(21) <223> Xaa = a range of amino acids between VL CDR3 and VH 25 CDR2

<220> <221> misc_feature <222> (39)..(39) 30 <223> Xaa = a range of amino acids between VH CDR2 and VH CDR3

<400> 360

35 Arg Ala Ser Ser Ser Val Ser Tyr Ile Val Xaa Gln Gln Trp Ser Phe 1 5 10 15

Asn Pro Pro Thr Xaa Ala Ile Tyr Pro Gly Asn Gly Glu Thr Ser Tyr 5 20 25 30 2021201261

Asn Gln Lys Phe Lys Gly Xaa Ser Tyr Tyr Ser Asn Ser Tyr Trp Tyr 10 35 40 45

Phe Asp Leu 50 15

<210> 361 <211> 51 <212> PRT 20 <213> Artificial sequence

<220> <223> Synthetic peptide

25 <220> <223> VLCDR1-X-VLCDR3-X- VHCDR2-X-VHCDR3

<220> 30 <221> misc_feature <222> (11)..(11) <223> Xaa = a range of amino acids between VL CDR1 and VL CDR3

35 <220> <221> misc_feature <222> (21)..(21)

<223> Xaa = a range of amino acids between VL CDR3 and VH CDR2

<220> 5 <221> misc_feature <222> (39)..(39) 2021201261

<223> Xaa = a range of amino acids between VH CDR2 and VH CDR3

10 <400> 361

Arg Ala Ser Ser Ser Val Ser Tyr Ile Val Xaa Gln Gln Tyr Ser Phe 1 5 10 15 15

Asn Pro Pro Thr Xaa Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr 20 25 30 20

Asn Gln Lys Phe Lys Gly Xaa Ser Tyr Tyr Ser Asn Ser Tyr Trp Tyr 35 40 45 25

Phe Asp Leu 50

30 <210> 362 <211> 51 <212> PRT <213> Artificial sequence 35 <220> <223> Synthetic peptide

<220> <223> VLCDR1-X-VLCDR3-X- VHCDR2-X-VHCDR3 5 <220> 2021201261

<221> misc_feature <222> (11)..(11) <223> Xaa = a range of amino acids between VL CDR1 and VL 10 CDR3

<220> <221> misc_feature <222> (21)..(21) 15 <223> Xaa = a range of amino acids between VL CDR3 and VH CDR2

<220> <221> misc_feature 20 <222> (39)..(39) <223> Xaa = a range of amino acids between VH CDR2 and VH CDR3

<400> 362 25 Arg Ala Ser Ser Ser Val Ser Tyr Ile His Xaa Gln Gln Trp Ser Phe 1 5 10 15

30 Asn Pro Pro Thr Xaa Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr 20 25 30

35 Asn Gln Lys Phe Lys Gly Xaa Ser Tyr Lys Ser Asn Ser Asp Trp Tyr

35 40 45

Phe Asp Leu 5 50 2021201261

<210> 363 <211> 51 10 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 15

<220> <223> VLCDR1-X-VLCDR3-X- VHCDR2-X-VHCDR3

20 <220> <221> misc_feature <222> (11)..(11) <223> Xaa = a range of amino acids between VL CDR1 and VL CDR3 25 <220> <221> misc_feature <222> (21)..(21) <223> Xaa = a range of amino acids between VL CDR3 and VH 30 CDR2

<220> <221> misc_feature <222> (39)..(39) 35 <223> Xaa = a range of amino acids between VH CDR2 and VH CDR3

<400> 363

Arg Ala Ser Ser Ser Val Ser Tyr Ile His Xaa Gln Gln Trp Ser Phe 5 1 5 10 15 2021201261

Asn Pro Pro Thr Xaa Ala Ile Tyr Pro Gly Asn Gly Glu Thr Ser Tyr 10 20 25 30

Asn Gln Lys Phe Lys Gly Xaa Ser Tyr Tyr Ser Asn Ser Asp Trp Tyr 15 35 40 45

Phe Asp Leu 50 20

<210> 364 <211> 51 <212> PRT 25 <213> Artificial sequence

<220> <223> Synthetic peptide

30 <220> <223> VLCDR1-X-VLCDR3-X- VHCDR2-X-VHCDR3

<220> 35 <221> misc_feature <222> (11)..(11) <223> Xaa = a range of amino acids between VL CDR1 and VL

CDR3

<220> <221> misc_feature 5 <222> (21)..(21) <223> Xaa = a range of amino acids between VL CDR3 and VH 2021201261

CDR2

<220> 10 <221> misc_feature <222> (39)..(39) <223> Xaa = a range of amino acids between VH CDR2 and VH CDR3

15 <400> 364

Arg Ala Ser Ser Ser Val Ser Tyr Ile His Xaa Gln Gln Tyr Ser Phe 20 1 5 10 15

Asn Pro Pro Thr Xaa Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr 25 20 25 30

Asn Gln Lys Phe Lys Gly Xaa Ser Tyr Tyr Ser Asn Ser Asp Trp Tyr 30 35 40 45

Phe Asp Leu 50 35 <210> 365 <211> 51

<212> PRT <213> Artificial sequence

<220> 5 <223> Synthetic peptide 2021201261

<220> <223> VLCDR1-X-VLCDR3-X- VHCDR2-X-VHCDR3 10 <220> <221> misc_feature <222> (11)..(11) <223> Xaa = a range of amino acids between VL CDR1 and VL 15 CDR3

<220> <221> misc_feature <222> (21)..(21) 20 <223> Xaa = a range of amino acids between VL CDR3 and VH CDR2

<220> <221> misc_feature 25 <222> (39)..(39) <223> Xaa = a range of amino acids between VH CDR2 and VH CDR3

<400> 365 30 Arg Ala Ser Ser Ser Val Ser Tyr Ile His Xaa Gln Gln Tyr Ser Phe 1 5 10 15

35 Asn Pro Pro Thr Xaa Ala Ile Tyr Pro Gly Asn Gly Glu Thr Ser Tyr

20 25 30

Asn Gln Lys Phe Lys Gly Xaa Ser Tyr Lys Ser Gly Gly Asp 5 Trp Tyr 35 40 45 2021201261

Phe Asp Leu 10 50

<210> 366 <211> 10 15 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 20

<220> <223> partial CH3 sequence

25 <400> 366

Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 1 5 10

30 <210> 367 <211> 9 <212> PRT <213> Artificial sequence 35 <220> <223> Synthetic peptide

<220> <223> Partial CH3 sequence 5 <400> 367 2021201261

Gln Lys Ser Leu Ser Leu Ser Pro Gly 1 5 10

<210> 368 <211> 1 <212> PRT 15 <213> Artificial sequence

<220> <223> Synthetic peptide

20 <220> <223> Partial CH3 sequence

<400> 368 25 Gln 1

30 <210> 369 <211> 3 <212> PRT <213> Artificial sequence

35 <220> <223> Synthetic peptide

<220> <223> Partial CH3 sequence

5 <400> 369 2021201261

Gln Lys Ser 1

10 <210> 370 <211> 6 <212> PRT <213> Artificial sequence 15 <220> <223> Synthetic peptide

20 <220> <223> Partial CH3 sequence

<400> 370

25 Gln Lys Ser Leu Ser Leu 1 5

<210> 371 30 <211> 5 <212> PRT <213> Artificial sequence

<220> 35 <223> Synthetic peptide

<220> <223> Partial CH3 sequence

<400> 371 5 Gln Lys Ser Leu Ser 2021201261

1 5

10 <210> 372 <211> 5 <212> PRT <213> Artificial sequence

15 <220> <223> Synthetic peptide

<220> 20 <223> Scorpion linker

<400> 372

Gly Cys Pro Pro Cys 25 1 5

<210> 373 <211> 12 30 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 35

<220>

<223> Scorpion linker

<400> 373

5 Gln Tyr Asn Cys Pro Gly Gln Tyr Thr Phe Ser Met 1 5 10 2021201261

<210> 374 10 <211> 11 <212> PRT <213> Artificial sequence

<220> 15 <223> Synthetic peptide

<220> <223> Scorpion linker 20 <400> 374

Pro Phe Phe Thr Cys Gly Ser Ala Asp Thr Cys 1 5 10 25

<210> 375 <211> 18 <212> PRT 30 <213> Artificial sequence

<220> <223> Synthetic peptide

35 <220> <223> Scorpion linker

<400> 375

Glu Pro Ala Phe Thr Pro Gly Pro Asn Ile Glu Leu Gln Lys 5 Asp Ser 1 5 10 15 2021201261

Asp Cys 10

<210> 376 <211> 18 15 <212> PRT <213> Artificial sequence

<220> <223> Synthetic peptide 20

<220> <223> Scorpion linker

25 <400> 376

Gln Arg His Asn Asn Ser Ser Leu Asn Thr Arg Thr Gln Lys Ala Arg 1 5 10 15 30

His Cys

35 <210> 377 <211> 17

<212> PRT <213> Artificial sequence

<220> 5 <223> Synthetic peptide 2021201261

<220> <223> Scorpion linker 10 <400> 377

Asn Ser Leu Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr 15 1 5 10 15

Cys

20

<210> 378 <211> 10 <212> PRT 25 <213> Artificial sequence

<220> <223> Synthetic peptide

30 <220> <223> anti-CD20 VL CDR1 (TRU-015)

<400> 378 35 Arg Ala Ser Ser Ser Val Ser Tyr Met His 1 5 10

<210> 379 <211> 13 5 <212> PRT <213> Artificial sequence 2021201261

<220> <223> Synthetic peptide 10

<220> <223> anti-CD20 VH CDR3 (TRU-015)

15 <400> 379

Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val 1 5 10

Claims (36)

1. A single-chain protein comprising from amino to carboxy terminus:

(a) a first binding domain derived from an immunoglobulin-like molecule or the variable regions of an immunoglobulin;

(b) a Fc region comprising a domain derived from an immunoglobulinCH2 domain, wherein said Fc region does not comprise a domain derived from an immunoglobulinCHi domain;

(c) a linker peptide of at least 5 amino acids; and

(d) a second binding domain derived from an immunoglobulin-like molecule or the variable regions of an immunoglobulin,

wherein the first or second binding domain binds OX40; and

wherein the first binding domain and the second binding domain recognize different molecular targets.

2. The protein according to claim 1, wherein the first and/or second binding domain is a single-chain variable antibody fragment (scFv).

3. The protein according to claim 2, wherein the scFv comprises SEQ ID NO: 2, 4, 6,103,105,107 or109.

4. The protein according to any one of claims 1-3, wherein the first and/or second binding domain comprises chimeric, humanized, or human immunoglobulin variable regions.

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5. The protein according to any one of claims 1-4, wherein the first and/or second binding domain comprises a light chain immunoglobulin variable region (VL1) and a heavy chain immunoglobulin variable region (VH1), wherein said variable regions are positioned in a VH1-VL1 or a VL-VH1 orientation.

6. The protein according to any one of claims 1-5, wherein the immunoglobulin like molecule is a receptor.

7. The protein according to any one of claims 1-6, wherein the binding domain that does not bind to OX40 binds to a target selected from the group consisting of a tumor antigen, a B-cell target, a TNF receptor superfamily member, a Hedgehog family member, a receptor tyrosine kinase, a proteoglycan-related molecule, a TGF-beta superfamily member, a Wnt-related molecule, a receptor ligand, a T-cell target, a Dendritic cell target, an NK cell target, a monocyte/macrophage cell target and an angiogenesis target.

8. The protein according to claim 7, wherein the tumor antigen is selected from the group consisting of SQUAMOUS CELL CARCINOMA ANTIGEN 1, SQUAMOUS CELL CARCINOMA ANTIGEN 2, Ovarian carcinoma antigen CA125, MUCIN 1, CTCL tumor antigen sel-1, CTCL tumor antigen sel4-3, CTCL tumor antigen se20-4, CTCL tumor antigen se20-9, CTCL tumor antigen se33-1, CTCL tumor antigen se37-2, CTCL tumor antigen se57-1, CTCL tumor antigen se89-1, Prostate-specific membrane antigen, 5T4 oncofetal trophoblast glycoprotein, Orf73 Kaposi's sarcoma-associated herpesvirus, MAGE-CI, MAGE-B ANTIGEN, MAGE-B2 ANTIGEN, MAGE-2 ANTIGEN, MAGE-4a antigen, MAGE-4b antigen, Colon cancer antigen NY-CO-45, Lung cancer antigen NY-LU- 12 variant A, Cancer associated surface antigen, Adenocarcinoma antigen ART1, Paraneoplastic associated brain-testis-cancer antigen, Neuro-oncological ventral antigen 2, Hepatocellular carcinoma antigen gene 520, TUMOR-ASSOCIATED ANTIGEN CO-029, Tumor-associated antigen MAGE-X2,

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Synovial sarcoma X breakpoint 2, Squamous cell carcinoma antigen recognized by T cell, Serologically defined colon cancer antigen 1, Serologically defined breast cancer antigen NY-BR-15, Serologically defined breast cancer antigen NY-BR- 16, Chromogranin A; parathyroid secretory protein 1, DUPAN-2, CA 19-9, CA 72-4, CA 195 and L6.

9. The protein according to claim 7, wherein the B-cell target is selected from the group consisting of CD1O, CD19, CD20, CD21, CD22, CD23, CD24, CD37, CD38, CD39, CD40, CD72, CD73, CD74, CDw75, CDw76, CD77, CD78, CD79a/b, CD80, CD81, CD82, CD83, CD84, CD85, CD86, CD89, CD98, CD126, CD127, CDw130, CD138 and CDw15O.

10. The protein according to claim 7, wherein the TNF receptor superfamily member is selected from the group consisting of 4-1BB/TNFRSF9, NGF R/TNFRSF16, BAFF R/TNFRSF13C, Osteoprotegerin/TNFRSF1IB, BCMA/TNFRSF17, CD27/TNFRSF7, RANK/TNFRSF11A, CD30/TNFRSF8, RELT/TNFRSF19L, CD40/TNFRSF5, TACI/TNFRSF13B, DcR3/TNFRSF6B, TNF RI/TNFRSFA, DcTRAIL R1/TNFRSF23, TNF RII/TNFRSFlB, DcTRAIL R2/TNFRSF22, TRAIL R1/TNFRSF1OA, DR3/TNFRSF25, TRAIL R2/TNFRSF1OB, DR6/TNFRSF21, TRAIL R3/TNFRSF1OC, EDAR, TRAIL R4/TNFRSF1OD, Fas/TNFRSF6, TROY/TNFRSF19, GITR/TNFRSF18, TWEAK R/TNFRSF12, HVEM/TNFRSF14, XEDAR, Lymphotoxin beta R/TNFRSF3, 4-1BB Ligand/TNFSF9, Lymphotoxin, APRIL/TNFSF13, Lymphotoxin beta/TNF SF3, BAFF/TNFSF13C, OX40 Ligand/TNFSF4, CD27 Ligand/TNFSF7, TL1A/TNFSF15, CD30 Ligand/TNFSF8, TNF-alpha/TNFSF1A, CD40 Ligand/TNFSFS, TNF-beta/TNFSF1B, EDA-A2, TRAIL/TNFSF10, Fas Ligand/TNFSF6, TRANCE/TNFSF11, GITR Ligand/'TNFSF18, TWEAK/TNFSF12 and LIGHT/TNFSF14.

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11. The protein according to claim 7, wherein the T-cell target is selected from the group consisting of 2B4/SLAMF4, IL-2 R alpha, 4-1BB/TNFRSF9, IL-2 R beta, ALCAM, B7-1/CD80, IL-4 R, B7-H3, BLAME/SLAMF8, BTLA, IL-6 R, CCR3, IL-7 R alpha, CCR4, CXCRI/IL-8 RA, CCR5, CCR6, IL-10 R alpha, CCR7, IL-10 R beta, CCR8, IL-12 R beta 1, CCR9, IL-12 R beta 2, CD2, IL-13 R alpha 1, IL-13, CD3, CD4, ILT2/CD85j, ILT3/CD85k, ILT4/CD85d, ILT5/CD85a, Integrin alpha 4/CD49d, CD5, Integrin alpha E/CD103, CD6, Integrin alpha M/CD1Ib, CD8, Integrin alpha X/CD1Ic, Integrin beta 2/CD18, KIR/CD158, CD27/TNFRSF7, KIR2DL 1, CD28, KIR2DL3, CD30/TNFRSF8, KIR2DL4/CD158d, CD31/PECAM-1, KIR2DS4, CD40 Ligand/TNFSF5, LAG-3, CD43, LAIRi, CD45, LAIR2, CD83, Leukotriene B4 RI, CD84/SLAMF5, NCAM-L I, CD94, NKG2A, CD97, NKG2C, CD229/SLAMF3, NKG2D, CD2F-IO/SLAMF9, NT-4, CD69, NTB-A/SLAMF6, Common gamma Chain/IL-2 R gamma, Osteopontin, CRACC/SLAMF7, PD-1, CRTAM, PSGL-1, CTLA-4, RANK/TNFRSF1A, CX3CRI, CX3CLI, L-Selectin, CXCR3, SIRP beta 1, CXCR4, SLAM, CXCR6, TCCR/WSX-1, DNAM-1, Thymopoietin, EMMPRIN/CD147, TIM-1, EphB6, TIM-2, Fas/TNFRSF6, TIM-3, Fas Ligand/TNFSF6, TIM-4, Fc gamma RIII/CD16, TIM-6, GITR/TNFRSF18, TNF RI/TNFRSFiA, Granulysin, TNF RII/TNFRSFlB, HVEM/TNFRSF14, TRAIL Ri/TNFRSFiOA, ICAM-i/CD54, TRAIL R2/TNFRSFiOB, ICAM-2/CD102, TRAIL R3/TNFRSF1OC, IFN-gamma RI, TRAIL R4/TNFRSFIOD, IFN-gamma R2, TSLP, iL-i RI and TSLP R.

12. The protein according to claim 7, wherein the NK cell receptor is selected from the group consisting of 2B4/SLAMF4, KIR2DS4, CD155/PVR, KIR3DL1, CD94, LMIRi/CD300A, CD69, LMIR2/CD300c, CRACC/SLAMF7, LMIR3/CD300LF, DNAM-1, LMIR5/CD300LB, Fc epsilon RII, LMIR6/CD300LE, Fc gamma RI/CD64, MICA, Fc gamma RIIB/CD32b, MICB, Fc gamma RIIC/CD32c, MULT-1, Fc gamma RIIA/CD32a, Nectin-2/CD112, Fc gamma RIII/CD16, NKG2A, FcRHI/IRTA5, NKG2C, FcRH2/IRTA4, NKG2D, FcRH4/IRTAi, NKp30, FcRH5/IRTA2, NKp44, Fc Receptor-like 3/CD16-2, NKp46/NCRi, NKp80/KLRF1, NTB-A/SLAMF6, Rae-1, Rae-i alpha, Rae-i beta, Rae-i delta, H60, Rae- epsilon, ILT2/CD85j, Rae-i gamma,

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ILT3/CD85k, TREM-1, ILT4/CD85d, TREM-2, ILT5/CD85a, TREM-3, KIR/CD158, TREML1/TLT-1, KIR2DL1, ULBP-1, KIR2DL3, ULBP-2, KIR2DL4/CD158d and ULBP-3.

13. The protein according to claim 7, wherein the monocyte/macrophage cell target is selected from the group consisting of B7-1/CD80, ILT4/CD85d, B7-H1, ILT5/CD85a, Common beta Chain, Integrin alpha 4/CD49d, BLAME/SLAMF8, Integrin alpha X/CD1ic, CCL6/C1O, Integrin beta 2/CD18, CD155/PVR, Integrin beta 3/CD61, CD31/PECAM-1, Latexin, CD36/SR-B3, Leukotriene B4 RI, CD40/TNFRSF5, LIMPII/SR-B2, CD43, LMIR1/CD300A, CD45, LMIR2/CD300c, CD68, LMIR3/CD300LF, CD84/SLAMF5, LMIR5/CD300LB, CD97, LMIR6/CD300LE, CD163, LRP-1, CD2F-10/SLAMF9, MARCO, CRACC/SLAMF7, MD-1, ECF-L, MD-2, EMMPRIN/CD147, MGL2, Endoglin/CD105, Osteoactivin/GPNMB, Fc gamma RI/CD64, Osteopontin, Fc gamma RIIB/CD32b, PD L2, Fc gamma RIIC/CD32c, Siglec-3/CD33, Fc gamma RIIA/CD32a, SIGNR/CD209, Fc gamma RIII/CD16, SLAM, GM-CSF R alpha, TCCR/WSX-1, ICAM-2/CD102, TLR3, IFN-gamma RI, TLR4, IFN-gamma R2, TREM-1, IL-1 RII, TREM-2, ILT2/CD85j, TREM-3, ILT3/CD85k, TREML1/TLT-1, 2B4/SLAMF4, IL-10 R alpha, ALCAM, IL-10 R beta, Aminopeptidase N/ANPEP, ILT2/CD85j, Common beta Chain, ILT3/CD85k, Cl qR1/CD93, ILT4/CD85d, CCR1, ILT5/CD85a, CCR2, Integrin alpha 4/CD49d, CCR5, Integrin alpha M/CD1Ib, CCR8, Integrin alpha X/CD1Ic, CD155/PVR, Integrin beta 2/CD18, CD14, Integrin beta 3/CD61, CD36/SR-B3, LAIRi, CD43, LAIR2, CD45, Leukotriene B4 RI, CD68, LIMPII/SR-B2, CD84/SLAMF5, LMIR1/CD300A, CD97, LMIR2/CD300c, CD163, LMIR3/CD300LF, Coagulation Factor III/Tissue Factor, LMIR5/CD300LB, CX3CR1, CX3CLI, LMIR6/CD300LE, CXCR4, LRP-1, CXCR6, M-CSF R, DEP-i/CD148, MD-1, DNAM-1, MD-2, EMMPRIN/CD147, MMR, Endoglin/CD105, NCAM-L1, Fc gamma RI/CD64, PSGL-1, Fc gamma RIII/CD16, RP105, G-CSF R, L-Selectin, GM CSF R alpha, Siglec-3/CD33, HVEM/TNFRSF14, SLAM, ICAM-i/CD54, TCCR/WSX-1,ICAM-2/CD102,TREM-i,IL-6R,TREM-2,CXCRi/IL-8RA, TREM-3 and TREML1/TLT-1.

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14. The protein according to any one of claims 1-13, wherein the Fc region further comprises an immunoglobulin hinge region.

15. The protein according to claim 14, wherein the hinge region is a hinge region selected from the group consisting of IgGI, IgG2, IgG3, IgG4, IgE, IgA2, synthetic hinge and the hinge-like CH2 domain of IgM.

16. The protein according to claim 15, wherein the hinge region is an IgGIhinge region.

17. The protein according to claim 16, wherein the hinge region is a human IgGI hinge region with a mutation at one or two cysteine residues.

18. The protein according to any one of claims 1-17, wherein the Fc region further comprises a domain derived from an immunoglobulin CH3 domain.

19. The protein according to any one of claims 1-18, wherein the Fc region comprises a human immunoglobulin hinge region, CH2 domain, and CH3 domain.

20. The protein according to any one of claims 1-19, wherein the linker peptide is between 5 and 45 amino acids in length.

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21. The protein according to any one of claims 1-20, wherein the linker peptide comprises one or more GGGGS motifs.

22. The protein according to any one of claims 1-13, wherein the linker peptide comprises an immunoglobulin core hinge region.

23. The protein according to any one of claims 1-13, wherein the linker peptide is derived from a stalk region of a type II C-lectin.

24. The protein according to claim 23, wherein the type II C-lectin is selected from the group consisting of CD69, CD72, CD94, NKG2A, and NKG2D.

25. The protein according to any one of claims 1-13, wherein the linker peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO:111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 149, 151, 153, 155,157,159,161,163,165,167,169,231,233,235,237,239,241,243,245,247, 249,251,253,255,257,259,261,263,265,267,269,271,273,275,277,279,281, 287,289,297,305,307,309,310,311,313,314,315,316,317,318,319,320,321, 322,323,324,325,326,327,328,329,330,331,346,373,374,375,376,and377.

26. The protein according to any one of claims 1-13, wherein the linker peptide is derived from an interdomain region between the Ig V-like and Ig C-like region of CD2, CD4, CD22, CD33, CD48, CD58, CD66, CD80, CD86, CD150, CD166, or CD244.

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27. The protein according to any one of claims 1-26, wherein the protein is capable of forming dimers.

28. A method for treating a cell proliferation disorder, comprising administering to a subject in need thereof a protein according to any one of claims 1-27.

29. The method according to claim 28, wherein the cell proliferation disorder is a cancer or a tumor.

30. A method for treating an autoimmune disease, comprising administering to a subject in need thereof a protein according to any one of claims 1-27.

31. The method according to claim 30, wherein the autoimmune disease is rheumatoid arthritis, osteoarthritis, psoriatic arthritis, psoriasis, inflammatory bowel disease, Crohn's disease, ulcerative colitis, asthma, systemic lupus erythematosus (SLE), diabetes, multiple sclerosis, solid organ transplant rejection, or graft versus host disease (GVHD).

32. A nucleic acid encoding the protein of any one of claims 1-27.

33. A vector comprising the nucleic acid of claim 32.

34. A host cell comprising the nucleic acid of claim 32 or the vector of claim 33, wherein said host cell is not present in a human.

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35. A composition comprising the protein of any one of claims 1-27 and a pharmaceutically acceptable carrier.

36. Use of the protein of any one of claims 1-27 in the manufacture of a medicament.

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