AU2021105058A4 - Rapid visual colorimetric detection method for environmental pollutant sulfide ions based on peroxidase-like dnazyme - Google Patents
Rapid visual colorimetric detection method for environmental pollutant sulfide ions based on peroxidase-like dnazyme Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 40
- 239000003344 environmental pollutant Substances 0.000 title claims abstract description 24
- -1 sulfide ions Chemical class 0.000 title claims abstract description 23
- 230000000007 visual effect Effects 0.000 title claims abstract description 11
- 239000000243 solution Substances 0.000 claims abstract description 44
- 108091027757 Deoxyribozyme Proteins 0.000 claims abstract description 31
- 239000007853 buffer solution Substances 0.000 claims abstract description 23
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 claims abstract description 20
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 claims abstract description 14
- 231100000719 pollutant Toxicity 0.000 claims abstract description 13
- 238000010521 absorption reaction Methods 0.000 claims abstract description 9
- 238000004364 calculation method Methods 0.000 claims abstract description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M potassium hydroxide Substances [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
- 239000007983 Tris buffer Substances 0.000 claims description 4
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 claims description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 3
- 239000007836 KH2PO4 Substances 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 2
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 2
- 235000011007 phosphoric acid Nutrition 0.000 claims description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 2
- 235000011118 potassium hydroxide Nutrition 0.000 claims description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 2
- 235000017550 sodium carbonate Nutrition 0.000 claims description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 2
- 239000001509 sodium citrate Substances 0.000 claims description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 2
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 8
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 description 6
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 5
- 229910052717 sulfur Inorganic materials 0.000 description 5
- 239000011593 sulfur Substances 0.000 description 5
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002371 ultraviolet--visible spectrum Methods 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/251—Colorimeters; Construction thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/29—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection
- G01N21/293—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using visual detection with colour charts, graduated scales or turrets
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
- G01N33/0044—Sulphides, e.g. H2S
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
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Abstract
The present invention discloses a rapid visual colorimetric detection method for
environmental pollutant sulfide ions based on peroxidase-like DNAzyme. The detection
method includes the following steps: Si: preparing a buffer solution at first, and then preparing
a peroxidase-like DNAzyme solution using the buffer solution; S2: afterwards, taking out a
certain amount of the buffer solution obtained in step S, adding the peroxidase-like DNAzyme
solution obtained in step Si, then adding a to-be-detected sulfide ion solution, an ABTS
solution and an H202 solution, mixing uniformly and standing, and performing UV-Vis signal
detection; and S3: finally, establishing a standard curve, substituting a detected absorption
value obtained in step S2 for calculation, and quantitatively analyzing the sulfide ion pollutant
in the to-be-detected sulfide ion solution. The method has the advantages such as easy
preparation, rapid detection, low cost, low sample consumption and so on, provides a new
detection method for the rapid detection of sulfide ions in environmental systems, and
meanwhile widens the application of peroxidase-like DNAzyme in the field of analytical
chemistry.
1/1
FIGURES
0.4
0.2.
0.01-
400 420 440 460 480 Bo0
A/nm
Fig. 1
pH 4.0 5.0 6.0 7.0 8.0 9.0
Abs 0.180 0.211 0.271 0.402 0.432 0.182
Fig. 2
Concentration
0 0.5 1 2 5 10
(pmol/L)
Abs 0.812 0.787 0.770 0.746 0.682 0.613
Fig. 3
Description
1/1
0.4
0.2.
0.01- 400 420 440 460 480 Bo0
A/nm Fig. 1
pH 4.0 5.0 6.0 7.0 8.0 9.0
Abs 0.180 0.211 0.271 0.402 0.432 0.182
Fig. 2
Concentration 0 0.5 1 2 5 10 (pmol/L) Abs 0.812 0.787 0.770 0.746 0.682 0.613 Fig. 3
The present invention belongs to the technical field of sulfide ion detection, and specifically relates to a rapid visual colorimetric detection method for environmental pollutant sulfide ions based on peroxidase-like DNAzyme.
In the ecological environment, the discharge of sulfur-containing waste gas and waste liquid not only directly affects human health, but also induces chemical reactions under certain conditions, causing serious pollution such as acid rain. Therefore, a rapid detection method for sulfide ions is particularly important for real-time monitoring of sulfur-containing wastewater. So far, by adopting large-scale chromatographic and spectroscopic instrumental analysis methods, such as fluorescence spectrophotometry, UV-Vis spectrophotometry, ion selective electrode method and ion chromatography, highly sensitive and selective detection of sulfide ion pollutants can be achieved. The above-mentioned large-scale instrument detection method cannot meet the requirements of outdoor field detection, thus the application of the method in the actual detection of sulfide ion pollutants is limited. Meanwhile, sulfur-containing compounds in nature have complex forms, different sample sources, and huge differences in sulfur content in samples, so a single technique cannot be used for determination.
The colorimetric method has the advantages of rapid detection, visualization and low sample consumption, and is an ideal method for detecting pollutant sulfide ions. So far, there is no research report on colorimetric detection of pollutant sulfide ions based on peroxidase-like DNAzyme, so it is of great significance to study such method.
SUMMARY The present invention aims to overcome the defects in the prior art and provides a rapid visual colorimetric detection method for environmental pollutant sulfide ions based on peroxidase-like DNAzyme, which can quickly and highly sensitively detect pollutant sulfur ions.
The present invention adopts the following technical solutions: a rapid visual colorimetric detection method for environmental pollutant sulfide ions based on peroxidase-like DNAzyme includes the following steps: Si: preparing a buffer solution at first, and then preparing a peroxidase-like DNAzyme solution using the buffer solution; S2: afterwards, taking out a certain amount of the buffer solution obtained in step Si, adding the peroxidase-like DNAzyme solution obtained in step Si, then adding a to-be-detected sulfide ion solution, an ABTS solution and an H202 solution, mixing uniformly and standing, and performing UV-Vis signal detection; and S3: finally, establishing a standard curve, substituting a detected absorption value obtained in step S2 for calculation, and quantitatively analyzing the sulfide ion pollutant in the to-be-detected sulfide ion solution. Furthermore, the buffer solution in step Si is a Tris-HC-KOH buffer solution prepared from Tris, HCl and KOH, the concentration of the Tris-HCl-KOH buffer solution is 20 mM, and the pH is 4.0-9.0. The concentration of the peroxidase-like DNAzyme solution in step Si is 5-500 nM. Furthermore, the buffer solution in step Si is prepared from at least two of NaHCO3, Na2CO3, KH2PO4, K2HPO4, H3PO4, KOH, NaOH, Tris, HCl, citric acid and sodium citrate. Furthermore, the concentration of the sulfide ion solution is 0-10 [mol/L, the concentration of the ABTS solution is 0.5-20 mM, and the concentration of the H202 solution is 0.1-10 mM. Furthermore, the standing duration in step S2 is 1-10 mins. Compared with the prior art, the present invention has the following beneficial effects: the present invention establishes, perfects and develops a sensing analysis method of sulfide ion pollutant, and initiates a new rapid, highly sensitive and low-cost detection method, which is of great significance and high application value for improving existing detection technologies. In addition, in the standard-concentration mixed system of pollutant sulfide ion, ABTS solution and peroxidase-like DNAzyme prepared in the present invention, the peroxidase-like DNAzyme, having the activity of catalyzing hydrogen peroxide, can catalyze a hydrogen peroxide-ABTS system and cause a color change. Therefore, a semi-quantitative and qualitative detection of pollutant sulfide ions can be achieved by observing the color of the mixed system, and a standard curve can be established by measuring the ultraviolet absorption signal intensity to bring the UV-Vis absorption signal intensity value of the to-be-detected sample into the curve for calculation. Thus, the quantitative analysis of the pollutant sulfide ions in the to-be-detected sample is achieved, the advantages such as easy preparation, rapid detection, low cost and low sample consumption are achieved, a new method for the rapid detection of sulfide ions in the environmental system is provided, and meanwhile the application of peroxidase-like DNAzyme in the field of analytical chemistry is widened.
Fig. 1 is an ultraviolet absorption spectrum measured by different systems in Embodiment 1 of the present invention;
Fig. 2 is a schematic diagram of Abs values of UV-Vis detection signals of a mixed reaction system under different pH conditions in Embodiment 2 of the present invention; and
Fig. 3 is a schematic diagram of Abs values of UV-Vis absorption signal intensity detected in different mixed reaction systems in Embodiment 3 of the present invention.
Wherein, a represents a system containing ABTS, H202 and a peroxidase-like DNAzyme buffer solution; and b represents a system containing ABTS, H202, sulfide ions and a peroxidase-like DNAzyme buffer solution.
The present invention will be further described in detail below in conjunction with embodiments.
The raw materials used by the embodiments are described below:
Embodiment 1
A 24 pM peroxidase-like DNAzyme solution, a 100 mM ABTS solution, a 100 mM H202 solution and a 1 mM sulfide ion solution are prepared with a 20 mM Tris-HC-KOH buffer solution with a pH of 7.4, respectively.
1. 2840 pL of the above Tris-HCl-KOH buffer solution, 25 pL of the DNAzyme solution, 75 pL of the ABTS solution and 60 pL of the H202 solution are taken and added together to a detection cell to be mixed uniformly, then standing for 4 mins, and performing the UV-Vis signal measurement (line a in Fig. 1).
2. 2810 pL of the above Tris-HC-KOH buffer solution, 25 pL of the DNAzyme solution, 30 pL of the to-be-detected sulfide ion solution, 75 pL of the ABTS solution and 60 pL of the H202 solution are taken and added together to a detection cell to be mixed uniformly, standing for 4 mins, and performing the UV-Vis signal measurement (line b in Fig. 1).
It can be seen from Fig. 1 that when peroxidase-like DNAzyme, ABTS and hydrogen peroxide exist in the measurement system, the intensity of the UV-Vis absorption spectrum is high (line a), which indicates that the peroxidase-like DNAzyme can effectively catalyze the ABTS-hydrogen peroxide system to generate detection signals; and when sulfide ions exist, UV-Vis absorption spectrum signals significantly decrease (line b), thus the sulfide ion pollutant can be detected according to the signal changes.
Embodiment 2 Effect of pH on UV-Vis Absorption Signals
Mixed reaction systems with different pH values are prepared, and signal detection is performed using the same method as in Embodiment 1. Wherein, the buffer solution is Tris-HC-KOH, with the pH values of 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0 respectively. Abs values of the UV-Vis detection signals of the mixed reaction systems under different pH conditions are shown in Fig. 2.
It can be seen from Fig. 2 that when the pH value of the mixed luminescence system is 4.0-9.0, an obvious UV-Vis signal can be detected, and when the pH values of the mixed system are 7.0 and 8.0, the UV-Vis signal is relatively strong.
Embodiment 3
Sl. The peroxidase-like DNAzyme solution is mixed with the ABTS solution and the sulfide ion standard solutions of different concentrations, and a vortex oscillator is adopted for mixing; and
S2. The mixed solution obtained in step Sl is added to the detection cell, then the H202 solution is added, followed by standing, and after standing for 5 mins, the UV-Vis absorption signal detection is performed.
Wherein, in order to verify the influence of sulfide ions of different concentrations on the detection signal, the concentrations of sulfide ions in the mixed system prepared are respectively: 0 ptmol/L, 0.5 tmol/L, 1 tmol/L, 2 pnol/L, 5 pmol/L and 10 pmol/L. Meanwhile, the concentration of peroxidase-like DNAzyme in the mixed systems is 0.2 tM, the concentration of the H202 solution is 2 mM, and the concentration of the ABTS solution is 2.5 mM. The Abs values of the UV-Vis absorption signal intensity of different mixed reaction systems are detected and shown in Fig. 3.
The above embodiments describe the implementation of the present invention in detail, but the present invention is not limited to the above implementation. Within the knowledge scope of those skilled in the art, various changes can also be made without departing from the spirit of the present invention.
Claims (5)
1. A rapid visual colorimetric detection method for environmental pollutant sulfide ions based on peroxidase-like DNAzyme, including the following steps:
Sl: preparing a buffer solution at first, and then preparing a peroxidase-like DNAzyme solution using the buffer solution;
S2: afterwards, taking out a certain amount of the buffer solution obtained in step S, adding the peroxidase-like DNAzyme solution obtained in step Si, then adding a to-be-detected sulfide ion solution, an ABTS solution and an H202 solution, mixing uniformly and standing, and performing UV-Vis signal detection; and
S3: finally, establishing a standard curve, substituting a detected absorption value obtained in step S2 for calculation, and quantitatively analyzing the sulfide ion pollutants in the to-be-detected sulfide ion solution.
2. The rapid visual colorimetric detection method for environmental pollutant sulfide ions based on peroxidase-like DNAzyme according to claim 1, characterized in that the buffer solution in step S is a Tris-HCl-KOH buffer solution prepared from Tris, HCl and KOH, the concentration of the Tris-HCl-KOH buffer solution is 20 mM, the pH is 4.0-9.0, and the concentration of the peroxidase-like DNAzyme solution in step Si is 5-500 nM.
3. The rapid visual colorimetric detection method for environmental pollutant sulfide ions based on peroxidase-like DNAzyme according to claim 1, characterized in that the buffer solution in step S is prepared from at least two of NaHCO3,Na2CO3, KH2PO4, K2HPO4, phosphoric acid, KOH, NaOH, Tris, HCl, citric acid and sodium citrate.
4. The rapid visual colorimetric detection method for environmental pollutant sulfide ions based on peroxidase-like DNAzyme according to claim 1, characterized in that the concentration of the sulfide ion solution is 0-10 [mol/L, the concentration of the ABTS solution is 0.5-20 mM, and the concentration of the H202 solution is 0.1-10 mM.
5. The rapid visual colorimetric detection method for environmental pollutant sulfide ions based on peroxidase-like DNAzyme according to claim 1, characterized in that the standing duration in step S2 is 1-10 mins.
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