AU2021104906A4 - Application of a bile acid composite bacterial agent in the preparation of swine feed additives - Google Patents
Application of a bile acid composite bacterial agent in the preparation of swine feed additives Download PDFInfo
- Publication number
- AU2021104906A4 AU2021104906A4 AU2021104906A AU2021104906A AU2021104906A4 AU 2021104906 A4 AU2021104906 A4 AU 2021104906A4 AU 2021104906 A AU2021104906 A AU 2021104906A AU 2021104906 A AU2021104906 A AU 2021104906A AU 2021104906 A4 AU2021104906 A4 AU 2021104906A4
- Authority
- AU
- Australia
- Prior art keywords
- bile acid
- bacterial agent
- acid composite
- composite bacterial
- application according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 66
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical compound C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 title claims abstract description 55
- 239000003613 bile acid Substances 0.000 title claims abstract description 53
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 38
- 239000002131 composite material Substances 0.000 title claims abstract description 38
- 241000282898 Sus scrofa Species 0.000 title claims abstract description 14
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000003674 animal food additive Substances 0.000 title claims abstract description 7
- 239000000725 suspension Substances 0.000 claims abstract description 16
- 238000009395 breeding Methods 0.000 claims abstract description 14
- 230000001488 breeding effect Effects 0.000 claims abstract description 14
- 240000004476 Eucalyptus polybractea Species 0.000 claims abstract description 10
- 235000009683 Eucalyptus polybractea Nutrition 0.000 claims abstract description 10
- 241000606123 Bacteroides thetaiotaomicron Species 0.000 claims abstract description 9
- 241000606210 Parabacteroides distasonis Species 0.000 claims abstract description 9
- 239000000341 volatile oil Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000003085 diluting agent Substances 0.000 claims description 4
- 239000000654 additive Substances 0.000 claims description 2
- 230000000996 additive effect Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 9
- 210000004185 liver Anatomy 0.000 abstract description 6
- 239000008267 milk Substances 0.000 abstract description 5
- 210000004080 milk Anatomy 0.000 abstract description 5
- 235000013336 milk Nutrition 0.000 abstract description 5
- 239000003053 toxin Substances 0.000 abstract description 4
- 231100000765 toxin Toxicity 0.000 abstract description 4
- 108700012359 toxins Proteins 0.000 abstract description 4
- 210000000232 gallbladder Anatomy 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000008030 elimination Effects 0.000 abstract description 2
- 238000003379 elimination reaction Methods 0.000 abstract description 2
- 230000007413 intestinal health Effects 0.000 abstract description 2
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- 239000003921 oil Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000007127 saponification reaction Methods 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 206010012735 Diarrhoea Diseases 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 239000002244 precipitate Substances 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000004042 decolorization Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000020477 pH reduction Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- DKPMWHFRUGMUKF-UHFFFAOYSA-N (3alpha,5alpha,6alpha,7alpha)-3,6,7-Trihydroxycholan-24-oic acid Natural products OC1C(O)C2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)CC2 DKPMWHFRUGMUKF-UHFFFAOYSA-N 0.000 description 1
- RUDATBOHQWOJDD-UHFFFAOYSA-N (3beta,5beta,7alpha)-3,7-Dihydroxycholan-24-oic acid Natural products OC1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)CC2 RUDATBOHQWOJDD-UHFFFAOYSA-N 0.000 description 1
- JOYGXTIHTHBSOA-UHFFFAOYSA-N 1-(4-chlorophenyl)-3-thiophen-2-ylprop-2-en-1-one Chemical compound C1=CC(Cl)=CC=C1C(=O)C=CC1=CC=CS1 JOYGXTIHTHBSOA-UHFFFAOYSA-N 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- DGABKXLVXPYZII-UHFFFAOYSA-N Hyodeoxycholic acid Natural products C1C(O)C2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)CC2 DGABKXLVXPYZII-UHFFFAOYSA-N 0.000 description 1
- 206010062016 Immunosuppression Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 241000160321 Parabacteroides Species 0.000 description 1
- 208000005374 Poisoning Diseases 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- RUDATBOHQWOJDD-BSWAIDMHSA-N chenodeoxycholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)CC1 RUDATBOHQWOJDD-BSWAIDMHSA-N 0.000 description 1
- 229960001091 chenodeoxycholic acid Drugs 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 235000018823 dietary intake Nutrition 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 230000010235 enterohepatic circulation Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- DGABKXLVXPYZII-SIBKNCMHSA-N hyodeoxycholic acid Chemical compound C([C@H]1[C@@H](O)C2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)CC1 DGABKXLVXPYZII-SIBKNCMHSA-N 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000001256 steam distillation Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/168—Steroids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/413—Gall bladder; Bile
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/12—Antidiarrhoeals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
Abstract
The present invention discloses application of a bile acid composite bacterial agent
in the preparation of swine feed additives. The bile acid composite bacterial agent
comprises Parabacteroides distasonis bacterial suspension, Bacteroides thetaiotaomicron
bacterial suspension, bile acid and Eucalyptus polybractea essential oil. The composite
s bacterial agent has natural components, without toxic and side effect, can significantly
restore the intestinal health and non-specific immunity of sows, promote the elimination
of toxins from the body, increase sows' milk yield, enhance piglets' physical conditions,
increase their survival rates, repair the damage to liver and gallbladder, and increase the
breeding incomes.
Description
TECHNICAL FIELD The present invention generally relates to the field of poultry and livestock breeding, and particularly to application of a bile acid composite bacterial agent in the preparation of swine feed additives. BACKGROUND Nowadays, swine breeding is generally in large scale. In order to achieve high profits, farmers will choose to over-breed and give excessive feeds. These problems will cause chaos in the pig breeding environment and affect the health of swine. At present, the main problems of swine breeding include body poisoning, malnutrition, immunosuppression, and inflammation, etc. Especially for sows before parturition, the breeding environment problems can cause in vivo accumulation of toxins in swine, and develop metabolic diseases and nosotoxicosis, leading to the reduction of body's self-purification ability and weakening the physical condition of the piglets, and causing diarrhea, etc. The diarrhea of piglets may induce pathological changes of liver and kidneys and reduce the survival rate of piglets. Presently, antibiotics are often used to solve the problems of endotoxin accumulation and diarrhea in sows and piglets, but antibiotics can make the body to produce resistance, which is not conducive to the swine breeding. SUMMARY In order to solve the drawbacks and deficiencies of the prior art, the present invention provides the application of a bile acid composite bacterial agent in the preparation of swine feed additives. The bile acid composite bacterial agent can significantly improve the immunity of sows and restore the healthy liver and intestines of piglets. In order to achieve the object, the present invention adopts the following technical solutions: The present invention provides application of a bile acid composite bacterial agent in the preparation of swine feed additives. Further, the bile acid composite bacterial agent comprises Parabacteroides
distasonis bacterial suspension, Bacteroides thetaiotaomicron bacterial suspension, bile acid and Eucalyptus polybractea essential oil. Further, the volume-to-mass ratio of the Parabacteroides distasonis bacterial suspension, Bacteroides thetaiotaomicron bacterial suspension, bile acid and Eucalyptus polybractea essential oil is 2-3:2-4:2-3:1. Further, the total bacterial count of the bile acid composite bacterial agent is not less than 6x108 CFU/mL. Further, the method of using the bile acid composite bacterial agent is: adding the bile acid composite bacterial agent directly to a feed for prenatal sow breeding, 3 times a day until sows give birth. Further, the additive amount of the bile acid composite bacterial agent is 2-2.5 L/t feeds for breeding. Further, after the bile acid composite bacterial agent is diluted with water in a volume ratio of 1:1-2, the diluent of the bile acid composite bacterial agent is directly administered to sick piglets, one daily for 8 to 12 consecutive days. Further, the amount of the diluent of the bile acid composite bacterial agent is 6 to 10 mL/piglet. Compared with the prior art, the present invention has the following advantages and beneficial effects. The composite bacterial agent provided in the present invention has natural components, without toxic and side effect. It can significantly restore the intestinal health and non-specific immunity of the parturient sows, restore their body's self-purification ability, promote the elimination of toxins from the body and protect the health of sows, thereby reducing the problem of diarrhea caused by maternal toxin infection in piglets; in addition, it can effectively increase the sow's milk yield and ensure the nutritional intake of suckling piglets. The bile acid composite bacterial agent can be directly administered to sick piglets to enhance their physical conditions, increase their survival rate and milk intake, and restore their liver and intestine functions, thereby protecting the liver and gallbladder and repairing the damage to liver and gallbladder. In addition, it has the antibacterial, anti-inflammatory and deworming effects, to make the piglet skin red and bright, thereby increasing the income of swine breeding. Therefore, it has broad application prospects.
BRIEF DESCRIPTION OF THE DRAWINGS FIG.1 shows the effect of a bile acid composite bacterial agent on sows with tear
spots; the left figure indicates sow with tear spots, and the right figure indicates the sows after using a bile acid composite bacterial agent. DETAILED DESCRIPTION Example 1: 1. Preparation of Parabacteroides distasonis bacterial suspension (1) Inoculate Parabacteroides distasonis into a liquid nutrient medium (tryptone 15.0g, soytone 5.0g, sodium chloride 5.0g, sterile water 10OOmL, pH 7.4), then activate and cultivate for 48 h in an anaerobic environment at 37°C, to get Parabacteroides distasonis bacterial solution; (2) Add a sucrose solution with a mass concentration of 20% to the bacterial solution at a volume ratio of 1:1, mix well and centrifuge to obtain a bacterial strain precipitate; (3) Re-dissolve the above bacterial strain precipitate with a sucrose solution with a mass concentration of 10%, with a ratio of the volume of the sucrose solution to the mass of the bacterial strain precipitate of 2:1, mix well to obtain Parabacteroides distasonis bacterial suspension with bacterial count of not less than 2x10' CFU/mL. 2. Preparation of Bacteroides thetaiotaomicron bacterial suspension Bacteroides thetaiotaomicron was inoculated into GAM liquid medium and activated under anaerobic environment at 35°C for 48 h. 1 mL of the resulting activated bacterial solution was re-inoculated into 50 mL GAM liquid medium and cultured for 24 to 76 h, centrifuged and the bacterial strain precipitate was resuspended in sterile normal saline, and centrifuged again and resuspended in sterile normal saline for the second time, to obtain Bacteroides thetaiotaomicron bacterial suspension with bacterial count>1x109 CFU/mL. 3. Preparation of bile acid (1) Saponification: Add 1kg of porcine gall powder crushed through a 40-mesh sieve into a reactor, then add 1OL of sodium hydroxide solution with a mass concentration of 10%, heat and stir until boiling, and keep boiling and stir for 14h, then cool the saponification fluid until solid-liquid stratification, then remove the supernatant, to obtain the remaining solid of bile acid saponification product; (2) Decolorization: Add 6L of water to the bile acid saponification product, heat it to completely dissolve the bile acid saponification product, then pour it into a decolorization tank, add hydrogen peroxide and stir evenly, react at room temperature
for 24 h, then filter the reaction solution to an acidification tank, to obtain the filtrate; (3) Acidification: Cool the filtrate to room temperature, slowly add 10% hydrochloric acid solution while stirring, and stop adding acid when the solution pH reaches 3, and centrifuge and filter to obtain a white solid; (4) Purification: Rinse the white solids with water continuously, and complete the water rinsing when the filtered water is detected neutral; place the white solids after centrifugal filtration into an oven to obtain the crude bile acid; (5) Recrystallization: Place the crude bile acid into an extraction tank, add ethyl acetate of 10 times the weight of the crude bile acid to stir until dissolved completely, then add 0.08g of anhydrous sodium sulfate for dehydration, filter and collect the filtrate, concentrate the filtrate under reduced pressure and recover ethyl acetate to obtain liquid bile acid; (6) Drying: Place the liquid bile acid into an oven to obtain a bile acid finished product. The bile acid finished product contains 78.6% of hyocholic acid and hyodeoxycholic acid and 20.0% of chenodeoxycholic acid. 4. Preparation of Eucalyptus polybractea essential oil The Australian Eucalyptus polybractea leaves were placed into a dryer for drying after washing in a washing tower, then pulverized in a pulverizer, and then entered a steam distillation kettle, a condenser and an oil-water separator to separate the oil phase and water phase. The water phase entered an ether extractor to separate the waste water, the liquid containing ether entered the ether recovery tower to recover the ether. The recovered ether and oil phase liquid were added to a fixed-bed adsorption dessicator, to further remove the moisture in the oil phase. Finally the oil phase liquid was added to a vacuum rectifying tower for rectification to remove the impurity oil and obtain the Eucalyptus polybractea essential oil. The prepared Eucalyptus polybractea essential oil was stored in a sealed, opaque container and protected from light. 5. Preparation of bile acid composite bacterial agent The above prepared bile acid, Eucalyptus polybractea essential oil, Bacteroides thetaiotaomicron bacterial suspension and Parabacteroides distasonis bacterial suspension were mixed evenly at a mass-to-volume ratio of 2:1:2:2, to obtain a composite bacterial agent with the total bacterial count of no less than 6x108 CFU/mL. Example 2 A total of 536 pigs were bred in a pig farm. During the breeding period, pregnant
sows were found to have the tear spots (FIG. 1), and 30 of them were chosen to study the effect of the bile acid composite bacterial agent. Thirty sows were divided into different pigsties for breeding. The bile acid composite bacterial agent was mixed into the feed at a ratio of 2L/ton feed. The sows were fed 3 times a day, for 20 consecutive days, as shown in FIG.1. Twenty days later, the tear spots of sows alleviated apparently and even disappeared. After 20 day, the bile acid composite bacterial agent was evenly mixed into the feed at a ratio of 1 L/ton feed to consolidate the effect until the sows farrowed. Compared with the sows whose feeds were not added the bile acid composite bacterial agent, the experimental sows could produce more milk with obviously improved milk quality. The piglets produced by the experimental sows had obviously improved health conditions, with stronger vitality and brighter red skin than other newborn piglets, indicating that the bile acid composite bacterial agent of the present invention had good safety effects, could effectively treat the tear spots of sows and would not bring negative effects to sows and piglets. In addition, because the components are natural and endogenous substances, the bile acid composite bacterial agent of the present invention can be recycled in the body along with the enterohepatic circulation of bile acid, with stable effect; furthermore, it is not easy to be catabolized out of the body.
Claims (5)
1. Application of a bile acid composite bacterial agent in the preparation of swine feed additives.
2. The application according to claim 1, wherein the bile acid composite bacterial agent comprises Parabacteroides distasonis bacterial suspension, Bacteroides thetaiotaomicron bacterial suspension, bile acid and Eucalyptus polybractea essential oil.
3. The application according to claim 2, wherein the volume-to-mass ratio of the Parabacteroides distasonis bacterial suspension, Bacteroides thetaiotaomicron bacterial suspension, bile acid and Eucalyptus polybractea essential oil is 2-3:2-4:2-3:1.
4. The application according to claim 2, wherein the total bacterial count of the bile acid composite bacterial agent is not less than 6x108 CFU/mL. 5. The application according to claim 1, wherein the method of using the bile acid composite bacterial agent is: adding the bile acid composite bacterial agent directly to a feed for prenatal sow breeding, 3 times a day until sows give birth. 6. The application according to claim 5, wherein the additive amount of the bile acid composite bacterial agent is 2-2.
5 L/t feeds for breeding. 7. The application according to claim 1, wherein after the bile acid composite bacterial agent is diluted with water in a volume ratio of 1:1-2, the diluent of the bile acid composite bacterial agent is directly administered to sick piglets, one daily for 8 to 12 consecutive days. 8. The application according to claim 7, wherein the amount of the diluent of the bile acid composite bacterial agent is 6 to 10 mL/piglet.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2021104906A AU2021104906A4 (en) | 2021-08-04 | 2021-08-04 | Application of a bile acid composite bacterial agent in the preparation of swine feed additives |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2021104906A AU2021104906A4 (en) | 2021-08-04 | 2021-08-04 | Application of a bile acid composite bacterial agent in the preparation of swine feed additives |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2021104906A4 true AU2021104906A4 (en) | 2021-09-30 |
Family
ID=77857753
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2021104906A Ceased AU2021104906A4 (en) | 2021-08-04 | 2021-08-04 | Application of a bile acid composite bacterial agent in the preparation of swine feed additives |
Country Status (1)
Country | Link |
---|---|
AU (1) | AU2021104906A4 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114698730A (en) * | 2022-04-24 | 2022-07-05 | 长沙绿叶生物科技有限公司 | Compound bacterium preparation for sow midwifery and preparation method and application thereof |
-
2021
- 2021-08-04 AU AU2021104906A patent/AU2021104906A4/en not_active Ceased
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114698730A (en) * | 2022-04-24 | 2022-07-05 | 长沙绿叶生物科技有限公司 | Compound bacterium preparation for sow midwifery and preparation method and application thereof |
CN114698730B (en) * | 2022-04-24 | 2023-10-03 | 长沙绿叶生物科技有限公司 | Composite bacterial preparation for sow midwifery and preparation method and application thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2021104906A4 (en) | Application of a bile acid composite bacterial agent in the preparation of swine feed additives | |
CN112280720B (en) | Bacteroides thetaiotaomicron GL-02 and complex microbial inoculum compounded with bile acid and application thereof | |
US20220135616A1 (en) | Method for extracting useful substances from shrimp shells | |
CN109965148B (en) | Application of mulberry leaf extract in inhibiting freshwater fish culture stress, feed and feed preparation method | |
CN108056325B (en) | Sea water black porgy feed with immunity enhancing function | |
CN105248858A (en) | Pregnant sow fodder and preparation method and application thereof | |
CN106046188B (en) | A kind of preparation method of fucoidin | |
CN112401077B (en) | Application of bile acid complex microbial inoculant in preparation of pig feed additive | |
US10035750B2 (en) | Preparation method for polyunsaturated fatty acid-calcium | |
CN106590921B (en) | Production and processing technology of refined snake oil | |
CN107801867A (en) | A kind of large yellow croaker composite immune reinforcing agent and preparation method thereof | |
CN102887821A (en) | Method for extracting DHA (docosahexaenoic acid) through extraction and separation of marine microalgae fermentation liquid | |
CN113143971B (en) | Application of bile acid complex microbial inoculum in preparation of preparation for preventing and treating pet tear stains | |
CN109673857B (en) | Mitochondrion autophagy regulator for relieving oxidative stress of pigs | |
CN109007391B (en) | Complete formula feed for sows at late gestation period for preventing dystocia and feeding method of complete formula feed | |
US20220152124A1 (en) | Application of a bile acid composite bacterial agent in the preparation of feed additives for mutton sheep | |
CN113264980B (en) | High-purity tea seed meal protein and tea saponin and preparation method thereof | |
CN102133231A (en) | Product prepared by shark cartilage for supplementing calcium and promoting metabolism and development of cartilage and preparation method thereof | |
CN104829578A (en) | Clean preparation method of naringenin | |
CN109619306B (en) | Weaned piglet mitochondrial function protective agent | |
CN113817793A (en) | Production process for increasing astaxanthin content in haematococcus pluvialis by lutein | |
CN102178316B (en) | Technology for producing natural humic acid living body health strengthening beverage by utilizing coal | |
CN104187075B (en) | A kind of sow feed and preparation method thereof | |
CN110999836A (en) | Nutrition strengthening method for promoting growth of penaeus orientalis larvae | |
CN102357116B (en) | Healthcare product for mammitis of milch goats |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FGI | Letters patent sealed or granted (innovation patent) | ||
MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |