AU2021104597A4 - Agar-based natamycin antibacterial membrane and preparation method thereof - Google Patents
Agar-based natamycin antibacterial membrane and preparation method thereof Download PDFInfo
- Publication number
- AU2021104597A4 AU2021104597A4 AU2021104597A AU2021104597A AU2021104597A4 AU 2021104597 A4 AU2021104597 A4 AU 2021104597A4 AU 2021104597 A AU2021104597 A AU 2021104597A AU 2021104597 A AU2021104597 A AU 2021104597A AU 2021104597 A4 AU2021104597 A4 AU 2021104597A4
- Authority
- AU
- Australia
- Prior art keywords
- natamycin
- agar
- parts
- membrane
- antibacterial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- NCXMLFZGDNKEPB-FFPOYIOWSA-N natamycin Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C[C@@H](C)OC(=O)/C=C/[C@H]2O[C@@H]2C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 NCXMLFZGDNKEPB-FFPOYIOWSA-N 0.000 title claims abstract description 92
- 229960003255 natamycin Drugs 0.000 title claims abstract description 92
- 235000010298 natamycin Nutrition 0.000 title claims abstract description 92
- 239000004311 natamycin Substances 0.000 title claims abstract description 92
- 229920001817 Agar Polymers 0.000 title claims abstract description 87
- 239000008272 agar Substances 0.000 title claims abstract description 87
- 239000012528 membrane Substances 0.000 title claims abstract description 86
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 66
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 63
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000012153 distilled water Substances 0.000 claims abstract description 24
- 108010010803 Gelatin Proteins 0.000 claims abstract description 21
- 239000008273 gelatin Substances 0.000 claims abstract description 21
- 229920000159 gelatin Polymers 0.000 claims abstract description 21
- 235000019322 gelatine Nutrition 0.000 claims abstract description 21
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 21
- 239000000843 powder Substances 0.000 claims abstract description 18
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 239000000243 solution Substances 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 15
- 238000001035 drying Methods 0.000 claims description 13
- 239000011521 glass Substances 0.000 claims description 12
- 239000007864 aqueous solution Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 7
- 238000005303 weighing Methods 0.000 claims description 5
- 239000003242 anti bacterial agent Substances 0.000 abstract description 14
- 235000013305 food Nutrition 0.000 abstract description 12
- 239000000758 substrate Substances 0.000 abstract description 4
- 238000009447 edible packaging Methods 0.000 abstract description 2
- 239000004014 plasticizer Substances 0.000 abstract description 2
- 239000012744 reinforcing agent Substances 0.000 abstract description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 8
- 238000004806 packaging method and process Methods 0.000 description 6
- 239000004599 antimicrobial Substances 0.000 description 5
- 241000228245 Aspergillus niger Species 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 230000001988 toxicity Effects 0.000 description 4
- 231100000419 toxicity Toxicity 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 239000005003 food packaging material Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000228212 Aspergillus Species 0.000 description 1
- 102000020897 Formins Human genes 0.000 description 1
- 108091022623 Formins Proteins 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 238000009452 anti-microbial packaging Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920002988 biodegradable polymer Polymers 0.000 description 1
- 239000004621 biodegradable polymer Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000013409 condiments Nutrition 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000003120 macrolide antibiotic agent Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 150000004291 polyenes Chemical class 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000013112 stability test Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/12—Agar or agar-agar, i.e. mixture of agarose and agaropectin; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/16—Nitrogen-containing compounds
- C08K5/17—Amines; Quaternary ammonium compounds
- C08K5/175—Amines; Quaternary ammonium compounds containing COOH-groups; Esters or salts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/34—Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/24—Adaptations for preventing deterioration or decay of contents; Applications to the container or packaging material of food preservatives, fungicides, pesticides or animal repellants
- B65D81/28—Applications of food preservatives, fungicides, pesticides or animal repellants
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/12—Agar-agar; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2489/00—Characterised by the use of proteins; Derivatives thereof
- C08J2489/04—Products derived from waste materials, e.g. horn, hoof or hair
- C08J2489/06—Products derived from waste materials, e.g. horn, hoof or hair derived from leather or skin
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/05—Alcohols; Metal alcoholates
- C08K5/053—Polyhydroxylic alcohols
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2203/00—Applications
- C08L2203/16—Applications used for films
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L89/00—Compositions of proteins; Compositions of derivatives thereof
- C08L89/04—Products derived from waste materials, e.g. horn, hoof or hair
- C08L89/06—Products derived from waste materials, e.g. horn, hoof or hair derived from leather or skin, e.g. gelatin
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Manufacturing & Machinery (AREA)
- Agronomy & Crop Science (AREA)
- Materials Engineering (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an agar-based natamycin antibacterial membrane. Its raw materials are
composed of the following parts by weight: 66.0-66.5 parts of agar powder, 24.5-25.0 parts of
glycerol, 8.0-8.5 parts of gelatin, 0.60-0.70 parts of natamycin and 230-270 parts of distilled
water. In addition, the invention also discloses a preparation method of the agar-based
natamycin antibacterial membrane. In the invention, agar is used as a membrane forming
substrate, glycerol is used as a plasticizer, gelatin is used as a reinforcing agent, and natamycin
is used as an antibacterial agent to prepare the antibacterial membrane; and the prepared
antibacterial membrane has strong antibacterial property, can be used for foods which need to
be stored under a low-moisture condition, enriches the types of edible packaging membranes
and has wide application prospect.
Description
Agar-based natamycin antibacterial membrane and preparation method thereof
The invention belongs to that technical field of food packaging material, and particularly
relates to an agar-based natamycin antibacterial membrane and a preparation method
thereof.
Natamycin has a polyene macrolide structure and is a natural biological antibacterial
agent that can effectively inhibit fungi such as molds and yeasts. Natamycin has the
characteristics of high efficiency, non-toxicity, safety, and broad spectrum. It can replace
traditional chemical antibacterial agents. It can be widely used in dairy products, meat
and its products, baked food, fruit and its products, and condiments. In addition, there are
also studies in the field of food packaging
Agar is a biodegradable polymer material and also a hydrophilic colloid, which has
certain application value in many fields. Because it can improve the texture of food,
enhance the quality of food, and does not have any toxicity, it is often used as an additive
in the research and development and production of various types of food, and the research
on food packaging has made some progress by using its gel properties.
Antimicrobial packaging is one of the more widely used food packaging, with a variety of
forms, such as adding volatile antimicrobial agents in packaging bags, adding volatile
antimicrobial agents or non-volatile antimicrobial agents to food packaging materials, or
attaching antimicrobial agents to the surface of packaging materials. The compound use
of the membrane-forming base material and the antibacterial agent has two advantages
that firstly, the membrane-forming base material serves as a carrier of the antibacterial agent and plays a role of slow release for releasing the antibacterial agent so that the antibacterial effect can be realized for a long time; On the other hand, although some membrane-forming substrate has a certain antibacterial property, its antibacterial range is narrow, and the selection of an appropriate antibacterial agent can complement the antibacterial spectrum of the membrane-forming substrate, so that the antibacterial membrane has a broad-spectrum antibacterial ability.
Although some research progress has been made in the application of antimicrobial
membranes, there are still some problems to be solved, which are as follows: 1. Some
antimicrobial agents have certain toxicity, causing certain hidden dangers to food safety;
2. under normal circumstances the addition of antibacterial agent will cause damage to
the structure of the membrane, reduce its mechanical strength; 3. Most studies are only
limited to the bacteriostasis of antibacterial membranes, but the release rules of
antibacterial agents have not been studied. In that invention, agar, glycerol and gelatin are
blend through interaction among different molecules by adopt a blending processing
technology to prepare an agar base membrane, and natamycin is added as an antibacterial
agent; and the prepared antibacterial membrane can effectively inhibit the breed of mold
and yeast and enrich the types of packaging membranes.
The invention provides a preparation method of an agar-based natamycin antibacterial
membrane, which solves the problems that a part of antibacterial agents have certain
toxicity in the prior art, so that a certain hidden danger is caused to food safety, and the
structure of the membrane is damaged due to the addition of the antibacterial agents
under general conditions, so that the mechanical strength is reduced.
The first object of the invention is to provide an agar-based natamycin antibacterial
membrane, which is prepared from the following raw materials in parts by weight: 66.0
66.5 parts of agar powder, 24.5-25.0 parts of glycerol, 8.0-8.5 parts of gelatin, 0.60-0.70
parts of natamycin and 230-270 parts of distilled water.
Preferably, the preparation raw material of the agar-based natamycin antibacterial
membrane comprises the following components in parts by weight: 66.2 parts of agar
powder, 24.8 parts of glycerol, 8.3 parts of gelatin, 0.66 part of natamycin and 250 parts
of distilled water.
A second object of the present invention is to provide a method for preparing an agar
based natamycin antibacterial membrane comprising the steps of:
Step 1, weighing 66.0-66.5 parts of agar powder, 24.5-25.0 parts of glycerol, 8.0-8.5 parts
of gelatin, 0.60-0.70 parts of natamycin and 230-270 parts of distilled water according to
parts by weight for standby;
Step 2, taking out 90 percent of the distilled water weighed in the step 1, sequentially
adding the agar powder, glycerol and gelatin weighed in the step 1 into the distilled
water, stirring and dissolving at 100 deg c, and obtaining agar membrane solution after all
the components are dissolved;
Step 3, dissolving the natamycin weighed in the step 1 in the remaining 10 percent of
distilled water, stirring to dissolve, and obtaining a natamycin aqueous solution after the
natamycin is completely dissolved;
And Step 4, adding the natamycin aqueous solution prepared in the step 3 into the agar
membrane solution in the step 2, and uniformly stirring to obtain the solution of
natamycin agar membrane, uniformly pouring the solution on a glass plate, standing for min, then drying, taking out the glass plate after drying, and uncovering the membrane to obtain the agar-based natamycin antibacterial membrane, and storing the agar-based natamycin antibacterial membrane at normal temperature.
Preferably, the glass sheet has a size of 29 mm by 23 mm.
Preferably, the drying conditions in the step 4 are from 35 to 38°C for 6 to 6-10h
. Preferably, the thickness of the agar-based natamycin antibacterial membrane prepared in
the step 4 is 0.080 to 0.085 mm.
Compared with the prior art, the invention has the beneficial effects that:
1) The invention that taking natamycin as an antibacterial agent, can effectively inhibit
fungi such as mold, yeast and the like, and has the characteristics of high efficiency, non
toxicity, safety, broad spectrum and the like, and can replace the traditional chemical
antibacterial agent to be widely apply in the field of food packaging.
2) In the invention, the agar is used as a carrier of the natamycin of the antibacterial
agent, so that the slow release effect is played on the release of the antibacterial agent,
and the antibacterial effect can be realized for a long time; In addition, the agar also has a
certain antibacterial property, and after the membrane is formed, the antibacterial
spectrum of the agar can be complementary to that of the membrane forming substrate
natamycin, so that the prepared antibacterial membrane has broad-spectrum antibacterial
capability.
3) In the invention, agar is used as a membrane forming base material, glycerol is used as
a plasticizer, gelatin is used as a reinforcing agent, and natamycin is used as an
antibacterial agent to prepare the antibacterial membrane; and the prepared antibacterial
membrane has strong antibacterial property, can be used for foods which need to be stored under the condition of low moisture, enriches the types of edible packaging membranes and has wide application prospect.
In order to enable those skilled in the art to better understand the technical scheme of the
present invention to be implemented, the present invention is further described below
with reference to specific examples, but the examples are not meant to limit the present
invention.
The test methods, not specifically described in the examples below, were conducted
according to methods and conditions conventional in the art and the starting materials
used were commercially available.
Example 1
The invention relates to an agar-based natamycin antibacterial membrane, which is
prepared from the following raw materials in parts by weight: 66.2 parts of agar powder,
24.8 parts of glycerol, 8.3 parts of gelatin, 0.66 part of natamycin and 250 parts of
distilled water.
The specific preparation method is as follows:
Step 1, weighing 66.2 parts of agar powder, 24.8 parts of glycerol, 8.3 parts of gelatin,
0.66 part of natamycin and 250 parts of distilled water according to parts by weight for
standby;
Step 2, taking out 90 percent of the distilled water weighed in the step 1, sequentially
adding the agar powder, glycerol and gelatin weighed in the step 1 into the distilled
water, stirring and dissolving at 100°C, and obtaining agar membrane solution after all
the components are dissolved;
Step 3, dissolving the natamycin weighed in the step 1 in the remaining 10 percent of
distilled water, stirring to dissolve, and obtaining a natamycin aqueous solution after the
natamycin is completely dissolved;
And step 4, adding the natamycin aqueous solution prepared in the step 3 into the agar
membrane solution prepared in the step 2, uniformly stirring to obtain the natamycin agar
membrane solution, uniformly pouring the natamycin agar membrane solution on a glass
plate with the size of 29 mm by 23 mm, standing for 10min, drying at 35°C for 8h, taking
out the glass plate after drying, and uncovering the membrane to obtain the agar-based
natamycin antibacterial membrane with the thickness of 0.084mm, and storing the agar
based natamycin antibacterial membrane at normal temperature.
Example 2
The invention relates to an agar-based natamycin antibacterial membrane, which is
prepared from the following raw materials in parts by weight: 66.0 parts of agar powder,
24.5 parts of glycerol, 8.0 parts of gelatin, 0.60 parts of natamycin and 230 parts of
distilled water.
The specific preparation method is as follows:
Step 1, weighing 66.0 parts of agar powder, 24.5 parts of glycerol, 8.0 parts of gelatin,
0.60 part of natamycin and 230 parts of distilled water according to parts by weight for
standby;
Step 2, taking out 90 percent of the distilled water weighed in the step 1, sequentially
adding the agar powder, glycerol and gelatin weighed in the step 1 into the distilled
water, stirring and dissolving at 100°C, and obtaining agar membrane solution after all
the components are dissolved;
Step 3, dissolving the natamycin weighed in the step 1 in the remaining 10 percent of
distilled water, stirring to dissolve, and obtaining a natamycin aqueous solution after the
natamycin is completely dissolved;
And step 4, adding the natamycin aqueous solution prepared in the step 3 into the agar
membrane solution prepared in the step 2, uniformly stirring to obtain the natamycin agar
membrane solution, uniformly pouring the natamycin agar membrane solution on a glass
plate with the size of 29 mm by 23 mm, standing for 10min, drying at 37°C for 10h,
taking out the glass plate after drying, and uncovering the membrane to obtain the agar
based natamycin antibacterial membrane with the thickness of 0.080mm, and storing the
agar-based natamycin antibacterial membrane at normal temperature.
Example 3
The invention relates to an agar-based natamycin antibacterial membrane, which is
characterized in that the preparation raw materials comprise the following components in
parts by weight: 66.5 parts of agar powder, 25.0 parts of glycerol, 8.5 parts of gelatin,
0.70 parts of natamycin and 270 parts of distilled water.
The specific preparation method is as follows:
Step 1, weighing 66.5 parts of agar powder, 25.0 parts of glycerol, 8.5 parts of gelatin,
0.70 part of natamycin and 270 parts of distilled water according to parts by weight for
standby;
Step 2, taking out 90 percent of the distilled water weighed in the step 1, sequentially
adding the agar powder, glycerol and gelatin weighed in the step 1 into the distilled
water, stirring and dissolving at 100°C, and obtaining agar membrane solution after all
the components are dissolved;
Step 3, dissolving the natamycin weighed in the step 1 in the remaining 10 percent of
distilled water, stirring to dissolve, and obtaining a natamycin aqueous solution after the
natamycin is completely dissolved;
And step 4, adding the natamycin aqueous solution prepared in the step 3 into the agar
membrane solution prepared in the step 2, uniformly stirring to obtain the natamycin agar
membrane solution, uniformly pouring the natamycin agar membrane solution on a glass
plate with the size of 23 mm by 29 mm, standing for10mmin, drying at 38°C for 6h, taking
out the glass plate after drying, and uncovering the membrane to obtain the agar-based
natamycin antibacterial membrane with the thickness of 0.085mm, and storing the agar
based natamycin antibacterial membrane at normal temperature.
In Examples 1 to 3, agar-based natamycin antibacterial membranes with good
performances were prepared, and the performances of the prepared agar-based natamycin
antibacterial membranes were tested below to explain the effects of the invention,The
specific test processes and results are shown below.
I. Performance test of agar-based natamycin antibacterial membrane
The performance of the agar-based natamycin antibacterial membrane prepared in
examples 1 to 3 was tested, and the specific results are shown in table 1.
Table 1 Performance parameters of agar-based natamycin antibacterial membrane
Total chromat Haze Transmitt Gloss Water Degradation rate Project Thickness ic (%) ance (Gs) solubility within 60 d in aberrati (%) soil (%) on Example 1 0.084 6.29 21.28 89.83 53.77 27.16 58.37 Example 2 0.080 6.26 21.20 89.90 53.82 26.92 59.02 Example 3 0.085 6.26 21.26 90.13 53.79 27.12 58.86
The agar-based natamycin antibacterial membrane prepared in example 1-3 were tested
for tensile strength and oxygen permeability coefficient at 20%, 50% and 90% relative
humidity, respectively,that specific results are shown in table 2.
Table 2 Test results
Oxygen permeability coefficient Tensilestrength(MPa) [cm3 cm/(cm 2 s-Pa)] Project Humidity Humidity Humidity Humidity Humidity Humidity 20% 50% 90% 20% 50% 90% Example 1 24.20 17.05 5.73 0.040e- 0.446e- 13.243e Example 2 24.18 16.95 5.62 0.044e- 15 0.452e- 15 13.682e-1 5 Example 3 24.26 17.12 5.78 0.040e- 15 0.444e- 15 13.153e-1 5 As can be seen from Table 1 and Table 2, the agar-based natamycin antibacterial
membranes prepared in Examples 1to 3 were good in all aspects and were able to meet
the use requirements.
II. Bacteriostasis test of agar-based natamycin antibacterial membrane
Inhibition effect on fungi: The low-temperature preserved yeast strain and Aspergillus
niger strain were selected in the experiment for inhibition test, and the yeast strain was
inoculated in PDA solution medium and cultured for two to three generations at 28±1°C
for standby; Aspergillus niger was placed in normal saline with the mass fraction of 0.9%
for shaking and crushing for standby.
The yeast and Aspergillus niger were gradient diluted with 0.9% normal saline to the
ideal concentration of 106 fu/g, and then 150 pL of bacterial solution was sucked and
coated on PDA solid medium. The antibacterial membrane was cut into small circles with
a diameter of 6mm and placed in the center of the medium,The yeasts were cultured at
28±1°C for 48 h and Aspergillus niger at 28±1°C for 72 h,After the culture, the diameter
of the antibacterial circle was measured with vernier caliper.
The results showed that the diameter of the inhibition zone of Aspergillus niger was
23.0810.002 mm, and that of yeast was 21.4670.002 mm, indicating a significant
inhibitory effect.
III. Stability test of agar-based natamycin antibacterial membrane
The agar-based natamycin antibacterial membranes prepared in Examples 1-3 were
respectively and flatly placed under the constant humidity conditions of relative humidity
of 20%, 50% and 90%, and the observation after 12 h revealed that the agar-based
natamycin antibacterial membranes were well preserved in a dry environment,It is easy to
be softened in a high humidity environment, but has no damage, which indicates that the
agar-based natamycin antibacterial membrane prepared by the invention has good
stability.
It should be noted that when referring to numerical ranges in the claims of the present
invention, it should be understood that two endpoints of each numerical range and any
numerical value between two endpoints can be selected,since the adopted step method is
the same as that of embodiments 1-3, the preferred embodiments are described in the
present invention in order to prevent redundancy,however, once the basic inventive
concept is known to those skilled in the art, additional changes and modifications can be
made to these embodiments. It is therefore intend that that appended claim be interpreted
as including the preferred embodiment and all such change and modifications as fall
within the scope of the present invention.
It will be apparent to those skilled in the art that various modifications and variations can
be made to the present invention without departing from the spirit and scope of the
invention. Thus, it is intended that the present invention include such modifications and variations as come within the scope of the invention as defined by the appended claims and their equivalents.
Claims (6)
1. The invention relates to an agar-based natamycin antibacterial membrane, which is
characterized in that the preparation raw materials comprise the following components in
parts by weight: 66.0-66.5 parts of agar powder, 24.5-25.0 parts of glycerol, 8.0-8.5 parts
of gelatin, 0.60-0.70 parts of natamycin and 230-270 parts of distilled water.
2. The agar-based natamycin antibacterial membrane according to claim 1, wherein the
preparation raw materials comprise the following components in parts by weight: 66.2
parts of agar powder, 24.8 parts of glycerol, 8.3 parts of gelatin, 0.66 part of natamycin
and 250 parts of distilled water.
3. A method for preparing an agar-based natamycin antibacterial membrane, comprising
the steps of:
Step 1, weighing 66.0-66.5 parts of agar powder, 24.5-25.0 parts of glycerol, 8.0-8.5 parts
of gelatin, 0.60-0.70 parts of natamycin and 230-270 parts of distilled water according to
parts by weight for standby;
Step 2, taking out 90 percent of the distilled water weighed in the step 1, sequentially
adding the agar powder, glycerol and gelatin weighed in the step 1 into the distilled
water, stirring and dissolving at 100°C, and obtaining agar membrane solution after all
the components are dissolved;
Step 3, dissolving the natamycin weighed in the step 1 in the remaining 10 percent of
distilled water, stirring to dissolve, and obtaining a natamycin aqueous solution after the
natamycin is completely dissolved;
And step 4, adding the natamycin aqueous solution prepared in the step 3 into the agar
membrane solution in the step 2, and uniformly stirring to obtain the natamycin agar membrane solution, uniformly pouring the natamycin agar membrane solution on a glass plate, standing for 10min, then drying, taking out the glass plate after drying, and uncovering the membrane to obtain the agar-based natamycin antibacterial membrane, and storing the agar-based natamycin antibacterial membrane at normal temperature.
4. The method for preparing an agar-based natamycin antibacterial membrane according
to claim 3, wherein the size of the glass plate is 29 mm by 23 mm ..
5. The preparation method of the agar-based natamycin antibacterial membrane according
to claim 3, wherein the drying condition in the step 4 is drying at 35-38°C for 6-10 h.
6. The method for preparing the agar-based natamycin antibacterial membrane according
to claim 3, wherein the thickness of the agar-based natamycin antibacterial membrane
prepared in the step 4 is 0.080-0.085 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2021104597A AU2021104597A4 (en) | 2021-07-27 | 2021-07-27 | Agar-based natamycin antibacterial membrane and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2021104597A AU2021104597A4 (en) | 2021-07-27 | 2021-07-27 | Agar-based natamycin antibacterial membrane and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2021104597A4 true AU2021104597A4 (en) | 2021-09-23 |
Family
ID=77745991
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2021104597A Ceased AU2021104597A4 (en) | 2021-07-27 | 2021-07-27 | Agar-based natamycin antibacterial membrane and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| AU (1) | AU2021104597A4 (en) |
-
2021
- 2021-07-27 AU AU2021104597A patent/AU2021104597A4/en not_active Ceased
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112553786A (en) | Antibacterial composite fiber membrane and preparation method and application thereof | |
| Han et al. | Characterization of physical, mechanical, and antioxidant properties of soy protein-based bioplastic films containing carboxymethylcellulose and catechin | |
| CN108070103B (en) | Degradable antibacterial composite preservative film and preparation method thereof | |
| CN101637294B (en) | Water-soluble soybean polysaccharide coating preservative | |
| Bharathi et al. | Nanofibre‐based bilayer biopolymer films: enhancement of antioxidant activity and potential for food packaging application | |
| CN110818955B (en) | MOF (Metal organic framework) proanthocyanidin-loaded antibacterial film and preparation method thereof | |
| CN113292753B (en) | Preparation method and application of controlled-release antibacterial chitosan-based composite membrane | |
| Sedlaříková et al. | Effect of oregano and marjoram essential oils on the physical and antimicrobial properties of chitosan based systems | |
| CN102326619A (en) | Vegetable and fruit coating preservative and preparation method thereof | |
| CN111154128B (en) | Antibacterial antioxidant whey protein isolate edible film and preparation method thereof | |
| CN113185734B (en) | Carboxymethyl chitosan/hydroformylation carboxymethyl cellulose composite membrane and preparation method thereof | |
| CN110818956A (en) | Chitosan-nano titanium dioxide composite membrane and preparation method thereof | |
| CN112409615A (en) | Preservative film containing peanut coat procyanidine inclusion compound and preparation method thereof | |
| Suresh et al. | Gum acacia/pectin/pullulan‐based edible film for food packaging application to improve the shelf‐life of ivy gourd | |
| CN109679355A (en) | A kind of antibacterial film and its application | |
| CN101565482B (en) | Novel PVA-based preserving and packaging coating material and preparation process thereof | |
| Huang et al. | Development and characterization of an edible chitosan–whey protein nano composite film for chestnut (Castanea mollissima Bl.) preservation | |
| AU2021104597A4 (en) | Agar-based natamycin antibacterial membrane and preparation method thereof | |
| CN109021262B (en) | Immobilized pickering emulsion antibacterial film and preparation method and application thereof | |
| CN109535492A (en) | A kind of preparation method of edibility bacteriostatic film | |
| CN108084818A (en) | Mould proof film and preparation method thereof | |
| CN105273243A (en) | Biodegradable film and preparation method thereof | |
| CN108559326B (en) | Mildew-proof preservative film and preparation method thereof | |
| CN110790987B (en) | Chitosan-silk fibroin edible film and preparation method thereof | |
| CN114437414A (en) | Wintergreen oil microcapsule composite preservative film and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) | ||
| MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |