AU2021104577A4 - Sdf-1 induced rabbit knee osteoarthritis model building device - Google Patents
Sdf-1 induced rabbit knee osteoarthritis model building device Download PDFInfo
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- AU2021104577A4 AU2021104577A4 AU2021104577A AU2021104577A AU2021104577A4 AU 2021104577 A4 AU2021104577 A4 AU 2021104577A4 AU 2021104577 A AU2021104577 A AU 2021104577A AU 2021104577 A AU2021104577 A AU 2021104577A AU 2021104577 A4 AU2021104577 A4 AU 2021104577A4
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- Australia
- Prior art keywords
- knee osteoarthritis
- sdf
- operation table
- building device
- water tank
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- 208000003947 Knee Osteoarthritis Diseases 0.000 title claims abstract description 25
- 201000008482 osteoarthritis Diseases 0.000 title claims abstract description 25
- 241000283973 Oryctolagus cuniculus Species 0.000 title claims abstract description 22
- 102100021669 Stromal cell-derived factor 1 Human genes 0.000 claims abstract description 16
- 101710088580 Stromal cell-derived factor 1 Proteins 0.000 claims abstract description 16
- 238000002347 injection Methods 0.000 claims abstract description 9
- 239000007924 injection Substances 0.000 claims abstract description 9
- 238000007493 shaping process Methods 0.000 claims abstract description 6
- 239000003621 irrigation water Substances 0.000 claims description 14
- 241001465754 Metazoa Species 0.000 claims description 5
- 230000002262 irrigation Effects 0.000 claims description 5
- 238000003973 irrigation Methods 0.000 claims description 5
- 241000195940 Bryophyta Species 0.000 claims description 3
- 210000003414 extremity Anatomy 0.000 claims description 3
- 235000011929 mousse Nutrition 0.000 claims description 3
- 238000003745 diagnosis Methods 0.000 abstract description 5
- 230000008506 pathogenesis Effects 0.000 abstract description 5
- 230000007246 mechanism Effects 0.000 abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 239000010865 sewage Substances 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 201000010099 disease Diseases 0.000 description 7
- 210000000629 knee joint Anatomy 0.000 description 7
- 239000008055 phosphate buffer solution Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- RNFNDJAIBTYOQL-UHFFFAOYSA-N chloral hydrate Chemical compound OC(O)C(Cl)(Cl)Cl RNFNDJAIBTYOQL-UHFFFAOYSA-N 0.000 description 4
- 229960002327 chloral hydrate Drugs 0.000 description 4
- 230000007850 degeneration Effects 0.000 description 4
- 210000001188 articular cartilage Anatomy 0.000 description 3
- 210000003141 lower extremity Anatomy 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 210000004417 patella Anatomy 0.000 description 3
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 2
- 241000283977 Oryctolagus Species 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000037005 anaesthesia Effects 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 210000001264 anterior cruciate ligament Anatomy 0.000 description 2
- 206010003246 arthritis Diseases 0.000 description 2
- 210000004439 collateral ligament Anatomy 0.000 description 2
- 229910052740 iodine Inorganic materials 0.000 description 2
- 239000011630 iodine Substances 0.000 description 2
- 230000005499 meniscus Effects 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 210000000426 patellar ligament Anatomy 0.000 description 2
- 210000002967 posterior cruciate ligament Anatomy 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 208000031873 Animal Disease Models Diseases 0.000 description 1
- 208000031295 Animal disease Diseases 0.000 description 1
- 208000000094 Chronic Pain Diseases 0.000 description 1
- 208000032170 Congenital Abnormalities Diseases 0.000 description 1
- 206010061619 Deformity Diseases 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 208000005137 Joint instability Diseases 0.000 description 1
- 102000055008 Matrilin Proteins Human genes 0.000 description 1
- 108010072582 Matrilin Proteins Proteins 0.000 description 1
- 206010052904 Musculoskeletal stiffness Diseases 0.000 description 1
- 208000008558 Osteophyte Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 206010067268 Post procedural infection Diseases 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 238000011558 animal model by disease Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000003321 cartilage cell Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 210000000281 joint capsule Anatomy 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 239000008176 lyophilized powder Substances 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 210000005065 subchondral bone plate Anatomy 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 201000004595 synovitis Diseases 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 210000004353 tibial menisci Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61G—TRANSPORT, PERSONAL CONVEYANCES, OR ACCOMMODATION SPECIALLY ADAPTED FOR PATIENTS OR DISABLED PERSONS; OPERATING TABLES OR CHAIRS; CHAIRS FOR DENTISTRY; FUNERAL DEVICES
- A61G13/00—Operating tables; Auxiliary appliances therefor
- A61G13/0036—Orthopaedic operating tables
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B50/00—Containers, covers, furniture or holders specially adapted for surgical or diagnostic appliances or instruments, e.g. sterile covers
- A61B50/10—Furniture specially adapted for surgical or diagnostic appliances or instruments
- A61B50/15—Mayo stands; Tables
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
- A61D2003/006—Appliances for supporting or fettering animals for operative purposes with leg or foot restraining means
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61G—TRANSPORT, PERSONAL CONVEYANCES, OR ACCOMMODATION SPECIALLY ADAPTED FOR PATIENTS OR DISABLED PERSONS; OPERATING TABLES OR CHAIRS; CHAIRS FOR DENTISTRY; FUNERAL DEVICES
- A61G13/00—Operating tables; Auxiliary appliances therefor
- A61G13/10—Parts, details or accessories
- A61G13/102—Fluid drainage means for collecting bodily fluids from the operating table, e.g. for blood, urine
Landscapes
- Health & Medical Sciences (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Endoscopes (AREA)
Abstract
OF THE DISCLOSURE
The present disclosure discloses an SDF-1 induced rabbit knee osteoarthritis model building
device. A rabbit is fixed in an inwards sunken groove of an operation table through fixing belts
of the building device, and then is operated with operation kits and injection syringes which are
collected in a collection drawer. The rabbit is effectively irrigated using a designed irrigating
water tank before and after the operation. Meanwhile, a designed drainage hole is used to collect
sewage, and a designed magnifying lens connected by a shaping rod is used to magnify and
display the injected part during injection. The present disclosure assists in building a reasonable
and effective knee osteoarthritis model to effectively study the pathogenesis mechanism of the
knee osteoarthritis, shorten the study period and provide reliable measures for effective diagnosis
and treatment.
1/1
DRAWINGS:
7 6 5
FIG. 1
Description
1/1
7 6 5
FIG. 1
SDF-1 INDUCED RABBIT KNEE OSTEOARTHRITIS MODEL BUILDING DEVICE
[0001] The present disclosure belongs to the technical field of animal disease modeling, and specifically relates to an SDF-1 induced rabbit knee osteoarthritis model building device.
[0002] Knee osteoarthritis is a disease that is widespread in the joints, and is the degeneration of the articular cartilage. Meanwhile, cartilage cells cannot repair the articular cartilage matrix, ending up with changes in composition and structure. This disease mainly clinically manifests as chronic pain, joint instability, stiffness, deformity and narrowing of the joint space. The pathological changes of the joint suffering from the knee osteoarthritis include degeneration of the articular cartilage, thickening of the subchondral bone, formation of osteophytes, changes in the degree of synovitis, degeneration of ligaments, degeneration of the meniscus of the knee joint, and hypertrophy of the joint capsule. Changes in the muscles, nerves, mucous sacs, and partial fat pads around the joints may also occur, leading to arthritis or arthritis symptoms. However, the pathogenesis of the knee osteoarthritis is not yet fully clear.
[0003] Animal disease models are applied to researches in experimental physiology, experimental pathology, experimental diagnostics, and therapeutics to explore the occurrence, development, diagnosis, treatment, etc. of diseases. They are an important part of modern medical research. The development process of the knee osteoarthritis disease is slow and complicated. Studies on the diseases relying on clinical cases alone or taking humans as experimental subjects develop slowly and are restrained in many aspects. By the use of experimental animal models to study the diseases, the occurrence mechanisms of the diseases can be observed more accurately, the development law of the diseases can be learned more effectively, and the advancement of medical technology can be promoted. Therefore, by establishing a reasonable and effective knee osteoarthritis animal model, the pathogenesis of the knee osteoarthritis can be accurately and effectively studied, the study period is shortened, and effective diagnosis and treatment methods can be sought.
[0004] The present disclosure aims to provide an SDF-1 induced rabbit knee osteoarthritis model building device which assists in building a reasonable and effective rabbit knee osteoarthritis model to effectively study the pathogenesis mechanism of knee osteoarthritis, shorten the study period and provide reliable measures for effective diagnosis and treatment.
[0005] In order to solve the above-mentioned problems, the technical solution adopted in the present disclosure is as follows.
[0006] The SDF-1 induced rabbit knee osteoarthritis model building device includes an animal fixing device which is an operation table provided with an inwards sunken groove in an upper end surface. A lower end surface of the operation table is provided with a collection drawer; several fixing belts used to fix limbs are provided on the inwards sunken groove of the operation table; an irrigation water tank is provided at one end of the right side of the operation table; the lower end of the irrigation water tank is connected with an irrigation pipe and head; one side of the irrigation water tank is connected with a magnifying lens through a shaping rod; one end of the operation table close to the irrigation water tank is provided with a run-through drainage hole; and the lower end of the drainage hole is connected with a guide pipe connected to a collection barrel at the bottom.
[0007] Further, the inwards sunken groove on the operation table is a slope, and the lowest point is provided with the drainage hole.
[0008] Further, the collection drawer collects injection syringes of different specifications, operation kits, and 2-0 Mousse stitches.
[0009] Beneficial effects:
[0010] The present disclosure provides the SDF-1 induced rabbit knee osteoarthritis model building device. A rabbit is fixed in the inwards sunken groove of the operation table through the fixing belts of the building device, and then is operated with the operation kits and the injection syringes which are collected in the collection drawer. The rabbit is effectively irrigated using the designed irrigating water tank before and after the operation. Meanwhile, the designed drainage hole is used to collect sewage, and the designed magnifying lens connected by the shaping rod is used to magnify and display the injected part during injection. The present disclosure assists in building the reasonable and effective knee osteoarthritis model to effectively study the pathogenesis mechanism of the knee osteoarthritis, shorten the study period and provide reliable measures for effective diagnosis and treatment.
[0011] FIG. 1 is a schematic structural diagram of an SDF-1 induced rabbit knee osteoarthritis model building device according to the embodiment of the present disclosure.
[0012] In the drawings, a list of components represented by the respective numerals is as follows:
[0013] 1: operation table; 2: inwards sunken groove; 3: collection drawer; 4: fixing belt; 5: irrigation water tank; 6: shaping rod; 7: magnifying lens; 8: irrigation pipe and head; 9: drainage hole; 10: collection barrel.
[0014] The invention content is explained in detail below with reference to the accompanying drawings.
[0015] Embodiment 1
[0016] An SDF-1 induced rabbit knee osteoarthritis model building device includes an animal fixing device which is an operation table 1 provided with an inwards sunken groove 2 in an upper end surface. A lower end surface of the operation table 1 is provided with a collection drawer 3; several fixing belts 4 used to fix limbs are provided on the inwards sunken groove 2 of the operation table 1; an irrigation water tank 5 is provided at one end of the right side of the operation table 1; the lower end of the irrigation water tank 5 is connected with an irrigation pipe and head 8; one side of the irrigation water tank 5 is connected with a magnifying lens 7 through a shaping rod 6; one end of the operation table 1 close to the irrigation water tank 5 is provided with a run-through drainage hole 9; and the lower end of the drainage hole 9 is connected with a guide pipe connected to a collection barrel 10 at the bottom.
[0017] The inwards sunken groove 2 on the operation table 1 is a slope, and the lowest point is provided with the drainage hole 9.
[0018] The collection drawer 3 collects injection syringes of different specifications, operation kits, and 2-0 Mousse stitches.
[0019] Embodiment2
[0020] In the present disclosure, 45 adult 3 to 5-month-old healthy Japanese white rabbits purchased by the Department of Experimental Zoology of Kunming Medical University, female or male or both, each 2 to 2.5 kg, are selected to verify the reliability of the present disclosure by means of grouping.
[0021] 1. Preparation of main ingredients
[0022] 1) SDF-1 solution: 1000 uL of PBS solution is added into 1000 ug of SDF-1 lyophilized powder to prepare SDF-1 stoste at 1000 ug/mL, and 0.2 mL of solutions at corresponding concentrations are prepared according to 10 ug/kg, 20 ug/kg, and 40 ug/kg.
[0023] 2) 10% chloral hydrate: The chloral hydrate is prepared according to a volume ratio of chloral hydrate anhydrous to normal saline of 1: 9.
[0024] 3) 0.01 mol/L phosphate buffer solution (PBS): PBS powder is dissolved in 2000 ml of distilled water, pH=7.4.
[0025] 4) preparation of 0.01 mol/LPBST: 0.1% Tween20 is added into the 0.01 mol/LPBS, and the product is used after full and uniform mixing.
[0026] 5) preparation of 5% sheep serum solution: a pipette sucks up 30 ul of TritonX-100 (0.3%); 500 ul of normal sheep serum stoste (5%) is used for closure; finally, the volume is fixed at 10 ml with the PBS; the solutions are uniformly mixed and then placed in a 4°C refrigerator for later application.
[0027] 2 Experimental method
[0028] 2.1 Building of a rabbit knee osteoarthritis model and animal grouping
[0029] 1) 45 3 to 5-month-old healthy Japanese white rabbits are randomly selected as a blank group, a Hulth operation group, a low-dosage group, a medium-dosage group, and a high-dosage group; the knee joint of the right posterior limb is an operation part; normal saline is injected to the blank group; the anterior cruciate ligament, the posterior cruciate ligament, and the medial collateral ligament of the Hulth operation group are cut off, and the medial meniscus of the Hulth operation group is excised; and the SDF-1 solution atlOug/kg, 20ug/kg, and 40ug/kg is injected to the low-dosage group, the medium-dosage group, and the high-dosage group respectively according to weights.
[0030] 2) Personnel should wear hats and masks in strict aseptic operation, and the 10% chloral hydrate is injected to the ear vein as per 1 mL/kg for anesthesia.
[0031] 4) After the anesthesia is completed, the rabbit is fixed on the operation table; rabbit hairs on the surface of the posterior limb are shaved with a shaver to expose the operation part; and the operation part is disinfected for 3 times with a sterile cotton ball dipped with Type I iodine.
[0032] 5) In the blank group, the articular cavity of the right knee joint is located and then punctured point by point; if a part is punctured through, it is indicated that this part is the articular cavity; 0.2 mL of normal saline is injected into the articular cavity; the prepared SDF-1 solution is injected into the corresponding articular cavities of the low, medium, and high-dosage groups; and the above-mentioned operations are repeated for all the injection groups on the 3rd day and the 5th day.
[0033] 6) In the Hulth operation group, the rabbit is fixed; after the right knee joint is disinfected and is covered with sterile drapes, the knee joint of the right posterior limb is cut with a scalpel from the center, forming a skin incision that is about 3 cm long to expose tissues such as the patella, the patellar tendon, and the muscles, and is then cut longitudinally along the patella and the patellar tendon to open the articular cavity; the knee joint is flexed, and the patella is eversed, fully exposing the articular cavity; the operation field is irrigated with the normal saline (irrigation is realized by the irrigation water tank); and the anterior cruciate ligament, the posterior cruciate ligament meniscus and the medial collateral ligament are cut off. The articular cavity is irrigated; the knee joint is reset; the operative incision is sutured layer by layer (the magnifying lens may be used in the injection and suturing processes); the operative wound is irrigated with the Type I iodine and covered with aseptic dressing; penicillin is injected daily according to 20,000 units/kg 3 days after the operation to prevent postoperative infection; and the dressing is changed once every other day to observe the wound healing.
[0034] 7) After the treatment, the rabbits are routinely fed in SPF animal rooms, and the wounds, activities and feeding are observed.
Claims (3)
1. An SDF-1 induced rabbit knee osteoarthritis model building device, comprising an animal fixing device which is an operation table provided with an inwards sunken groove in an upper end surface, wherein lower end surface of the operation table is provided with a collection drawer; several fixing belts used to fix limbs are provided on the inwards sunken groove of the operation table; an irrigation water tank is provided at one end of the right side of the operation table; the lower end of the irrigation water tank is connected with an irrigation pipe and head; one side of the irrigation water tank is connected with a magnifying lens through a shaping rod; one end of the operation table close to the irrigation water tank is provided with a run-through drainage hole; and the lower end of the drainage hole is connected with a guide pipe connected to a collection barrel at the bottom.
2. The SDF-1 induced rabbit knee osteoarthritis model building device according to claim 1, wherein the inwards sunken groove on the operation table is a slope, and the lowest point is provided with the drainage hole.
3. The SDF-1 induced rabbit knee osteoarthritis model building device according to claim 1, wherein the collection drawer collects injection syringes of different specifications, operation kits, and 2-0 Mousse stitches.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2021104577A AU2021104577A4 (en) | 2021-07-26 | 2021-07-26 | Sdf-1 induced rabbit knee osteoarthritis model building device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2021104577A AU2021104577A4 (en) | 2021-07-26 | 2021-07-26 | Sdf-1 induced rabbit knee osteoarthritis model building device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2021104577A4 true AU2021104577A4 (en) | 2021-10-28 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2021104577A Ceased AU2021104577A4 (en) | 2021-07-26 | 2021-07-26 | Sdf-1 induced rabbit knee osteoarthritis model building device |
Country Status (1)
| Country | Link |
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| AU (1) | AU2021104577A4 (en) |
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2021
- 2021-07-26 AU AU2021104577A patent/AU2021104577A4/en not_active Ceased
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| FGI | Letters patent sealed or granted (innovation patent) | ||
| MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |