AU2020103535A4 - A new cell absorption rate three-dimensional (3D) testing method enhanced by a common path F-P cavity - Google Patents
A new cell absorption rate three-dimensional (3D) testing method enhanced by a common path F-P cavity Download PDFInfo
- Publication number
- AU2020103535A4 AU2020103535A4 AU2020103535A AU2020103535A AU2020103535A4 AU 2020103535 A4 AU2020103535 A4 AU 2020103535A4 AU 2020103535 A AU2020103535 A AU 2020103535A AU 2020103535 A AU2020103535 A AU 2020103535A AU 2020103535 A4 AU2020103535 A4 AU 2020103535A4
- Authority
- AU
- Australia
- Prior art keywords
- cell
- tested
- cavity
- absorption rate
- distribution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000010521 absorption reaction Methods 0.000 title claims abstract description 87
- 238000012360 testing method Methods 0.000 title claims abstract description 31
- 238000000386 microscopy Methods 0.000 claims abstract description 15
- 238000005259 measurement Methods 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims abstract description 5
- 230000003287 optical effect Effects 0.000 claims description 40
- 230000009466 transformation Effects 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 2
- 238000002310 reflectometry Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 14
- 230000035945 sensitivity Effects 0.000 abstract description 12
- 238000003325 tomography Methods 0.000 abstract description 9
- 230000001066 destructive effect Effects 0.000 abstract description 4
- 238000003384 imaging method Methods 0.000 description 17
- BJQHLKABXJIVAM-UHFFFAOYSA-N bis(2-ethylhexyl) phthalate Chemical compound CCCCC(CC)COC(=O)C1=CC=CC=C1C(=O)OCC(CC)CCCC BJQHLKABXJIVAM-UHFFFAOYSA-N 0.000 description 14
- 238000010586 diagram Methods 0.000 description 8
- 239000011521 glass Substances 0.000 description 4
- 238000012576 optical tweezer Methods 0.000 description 4
- 241000223785 Paramecium Species 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000013307 optical fiber Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000001550 testis Anatomy 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000002591 computed tomography Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000009647 digital holographic microscopy Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000012014 optical coherence tomography Methods 0.000 description 1
- 230000010363 phase shift Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/04—Processes or apparatus for producing holograms
- G03H1/08—Synthesising holograms, i.e. holograms synthesized from objects or objects from holograms
- G03H1/0866—Digital holographic imaging, i.e. synthesizing holobjects from holograms
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
- G02B6/24—Coupling light guides
- G02B6/26—Optical coupling means
- G02B6/28—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals
- G02B6/293—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals with wavelength selective means
- G02B6/29346—Optical coupling means having data bus means, i.e. plural waveguides interconnected and providing an inherently bidirectional system by mixing and splitting signals with wavelength selective means operating by wave or beam interference
- G02B6/29358—Multiple beam interferometer external to a light guide, e.g. Fabry-Pérot, etalon, VIPA plate, OTDL plate, continuous interferometer, parallel plate resonator
- G02B6/29359—Cavity formed by light guide ends, e.g. fibre Fabry Pérot [FFP]
-
- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/04—Processes or apparatus for producing holograms
- G03H1/08—Synthesising holograms, i.e. holograms synthesized from objects or objects from holograms
- G03H1/0866—Digital holographic imaging, i.e. synthesizing holobjects from holograms
- G03H2001/0883—Reconstruction aspect, e.g. numerical focusing
Abstract
The present invention provides a new cell absorption rate three-dimensional (3D) testing method
enhanced by a common path F-P cavity. It is characterized by the composition of an F-P cavity
based digital hologram recording, numerical reconstruction, error processing, and a 3D
absorption rate distribution reconstruction. The present invention mainly provides a new cell
absorption rate 3D testing method enhanced by a common path F-P cavity, compared to the
conventional microscopy method, this has a higher sensitivity. The present invention has the
advantages of simple structure, high sensitivity and accurate measurement. The invention can be
used for high-resolution 3D microscopy of biological cells, which can be widely used for the
non-destructive, non-marking and non-contact 3D tomography and etc. of optically transparent
objects.
1/5
DRAWINGS
Record the digital hologram without placing
the cell to be tested, then place the cell to
be tested in the F-P cavity
i0=0
Record the optimal digital
hologram at the angel 0
Numerically reconstructing U,
FIT = Un -U *
AI -~T,
0<360°
A=Al/I0
0 3600
All angles' absorption rate distribution will be iRadon
transformed in turns and this can reconstruct and obtain the
3D absorption rate distribution of the cell to be tested.
FIG. 1
Description
1/5 DRAWINGS
Record the digital hologram without placing the cell to be tested, then place the cell to be tested in the F-P cavity
i0=0 Record the optimal digital hologram at the angel 0
Numerically reconstructing U,
FIT = Un -U *
AI -~T,
0<360°
A=Al/I0
0 3600 All angles' absorption rate distribution will be iRadon transformed in turns and this can reconstruct and obtain the 3D absorption rate distribution of the cell to be tested.
FIG. 1
A new cell absorption rate three-dimensional (3D) testing method enhanced by a common path
F-P cavity
[0001] The present invention relates to a new cell absorption rate 3D testing method enhanced by
a common path F-P cavity, which can be used for high-resolution 3D microscopy imaging of
biological cells, and can be widely used for the non-destructive, non-marking and non-contact
3D tomography and etc. of optically transparent objects, this belongs to the technical field of
microscopic imaging.
[0002] The 3D absorption rate distribution of cells to be tested is an important inherent property
of it. For optically transparent cells to be tested, the 3D absorption rate distribution reflects the
microstructure, density, and other information of the sample, thus enabling nondestructive, non
marking, and non-contact 3D tomography.
[0003] In contemporary life science research, fluorescence imaging is often used to mark the
samples to be tested. However, the process of marking can have an impact on the sample to be tested itself, which can affect the final results of the study. Digital holographic tomography, on the other hand, is a novel non-destructive, non-marking, and non-contact imaging technique that can reconstruct and obtain the 3D absorption rate distribution information on cells to be testes, which is a recent research interest.
[0004] Digital holographic tomography, which combines digital holographic microscopy and
computed tomography, is a new technique proposed in recent years. In recent years, although a
variety of imaging methods have been proposed to apply digital holographic tomography, most
of the ideas are to combine the Mach-Zehnder interferential optical path for digital holographic
recording.
[0005] Mach-Zehnder interferential optical path imaging uses more devices, requires higher
system stability, and becomes complex to operate. The Mach-Zehnder interferential optical path
based methods have higher device requirements and the optical path is more complex and
difficult to debug, thus there is an urgent need for a new imaging method that uses fewer devices,
simpler optical paths, higher system stability and easier operation, also it has a higher sensitivity
and higher imaging resolution for the measurement of cells to be tested.
[0006] The present invention presents a new cell absorption rate 3D testing method enhanced by
a common path F-P cavity, which uses an F-P cavity interferential optical path, which has a
simple optical path and uses fewer devices, results in a more stable system. In principle, the light
beam is reflected in the F-P cavity multiple times, and passes through the cell to be tested many
times; the cell to be tested absorbs light wave many times and the light intensity attenuation
accumulates in turns, the absorption is enhanced, hence the sensitivity of the measurement is
higher. The F-P cavity interference forms a hologram with a higher degree of fine detail
compared to the Mach-Zehnder interference, and the hologram has a more precise record.
[0007] Patent CN201310082100.9 discloses a digital holographic imaging online reconstruction display system and method, featuring a Mach-Zehnder interferometric digital holographic recording optical path, as compared to the new cell absorption rate 3D testing method enhanced by a common path F-P cavity proposed by the invention, the new method proposed by the present invention has a higher measurement sensitivity.
[0008] Patent CN201610911993.7 discloses a dual-wavelength phase microscopy system and method, as well as the corresponding phase recovery method, which is characterized by the use of a Mach-Zehnder interference optical path to achieve a dual-wavelength coaxial phase shift interference microscopy system, which is fundamentally different from the present invention, and is not as sensitive to measurement as the new method proposed herein.
[0009] Patent CN201710518263.5 discloses a 3D chromatography microscopy system and method, which can reproduce the three-dimensional refractive index information of the sample, but the imaging optical paths are fundamentally different from the present invention and the devices required are more complex, it is also difficult to obtain the 3D absorption rate distribution of the cells to be tested.
[0010] Patent CN201710904860.1 discloses an optical coherence tomography imaging system. The imaging system uses a Mach-Zehnder interferometric optical path, which features an optical fiber to simplify the system and reduce costs, but compared to the optical path structure of an F-P cavity, it is still quite complex.
[0011] Patent CN201810145657.5 discloses a high-resolution digital holographic diffraction tomography, which features a Mach-Zehnder interferometric optical path structure and uses a synthetic aperture method to obtain N synthetic high-resolution holograms, which in turn obtains a high-resolution three-dimensional refractive index reproduction of the measured sample. The structure is relatively more complex and is fundamentally different from the patent.
[0012] Patent CN201910136421.X discloses a super-resolution digital holographic imaging
system and imaging method featuring a light source modulated by adding a piece of transmissive
spatial light modulator in front of a conventional Mach-Zehnder interference optical path. This is
fundamentally different from the present invention which uses an F-P cavity optical path
structure.
[0013] The present invention discloses a new cell absorption rate 3D testing method enhanced by
a common path F-P cavity, which can be used for high-resolution microscopic imaging of
biological cells. This can be widely used in the field of non-destructive, non-labeled, non-contact
tomographic imaging of optically transparent objects, etc. This new cell absorption rate 3D
testing method enhanced by a common path F-P cavity employs digital holographic recording
optical path based on a common path F-P cavity and digital holographic tomography, to
reconstruct the tomography of the digital holograms recorded by the F-P cavity-based optical
path, and restore the 3D absorption rate distribution of the cell to be tested. Compared to the
previous technology, the measurement sensitivity is higher due to the employment of the digital
holographic recording optical path based on a common path F-P cavity, where the light beam is
reflected multiple times in the F-P cavity and passes through the cell to be tested. This new cell
absorption rate 3D testing method enhanced by a common path F-P cavity has the advantages of
simple structure, high sensitivity, and higher resolution.
[0014] It is an object of the present invention to provide a simple structure, high sensitivity, and
higher resolution new cell absorption rate 3D testing method enhanced by a common path F-P
cavity.
[0015] The object of the present invention is achieved by the following:
[0016] The new cell absorption rate 3D testing method enhanced by a common path F-P cavity
includes an F-P cavity-based digital hologram recording, numerical reconstruction, error
processing and 3D absorption rate distribution reconstruction. Record the digital hologram
without the sample of cell to be tested, then place the cell to be tested in the F-P cavity. When 0
=0, record the optimal digital hologram containing information on the sample of cell to be tested,
and the recorded digital hologram is numerically reconstructed to obtain the complex amplitude
distribution Ui of transmitted light, the complex amplitude distribution of the transmitted light
is multiplied by its conjugate to obtain the light intensity distribution of the transmitted light. Use
the light intensity distribution 1J without information on the sample of cell to be tested, to
subtract the light intensity distribution IT containing information on the sample of cell to be
tested, resulting in a absorption light intensity distribution AI that contains only information on
the sample of cell to be tested, and from the derived equation the absorption rate distribution A
at this angle can be achieved. Rotate the sample of cell to be tested, record the optimal digital
hologram at different angles, and sequentially obtain the absorption rate distribution containing
only information on the sample of cell to be tested at that angle. The high-resolution 3D
absorption rate distribution A of the sample of cell to be tested can be reconstructed by
performing an iRadon transformation of the absorption rate distribution at all angles.
[0017] The proposed new cell absorption rate 3D testing method enhanced by a common path F
P cavity is applicable to measurement systems based on a digital holographic recording optical
path based on a common path F-P cavity 11, a cell to be tested 12, a control module 13 and a
computational display module 14, as shown in FIG. 2a. The digital holographic recording optical
path based on a common path F-P cavity includes a light source, a beam expander, an F-P cavity,
a microscopy, an image acquire, and the like, all devices have a common path. The cell to be
tested can be biological cells or tiny objects. The control module is composed of a computer, a
device control unit and a device control interface to control and operate the image acquirer, the rotary control platform, etc., and to complete the recording of a digital hologram containing information on the cell to be tested. The computational display module performs program processing on the recorded digital hologram and displays online the information on the 3D absorption rate distribution of the cell to be tested.
[0018] The digital holographic recording optical path based on a common path F-P cavity 11 includes a light source, a beam expander, an F-P cavity, a microscopy, and an image acquirer, etc., all devices have a common path. Preferably, the light source is a laser light source with a wavelength of 532 nm, and a beam expander of the corresponding wavelength is selected, an F-P etalon with a fixed cavity length is selected, a microscopy with a magnification of 20x, and a Charge Coupled Device (CCD) with a pixel size of 3.45 pm x3.45 pm and other devices are selected.
[0019] The cell to be tested 12 is located in the F-P cavity of the digital holographic recording optical path based on a common path F-P cavity 11. The light beam is reflected in the F-P cavity multiple times and passes through the cell to be tested many times, the cell to be tested absorbs light wave many times and the light intensity attenuation accumulates in turns, the absorption is enhanced, hence the sensitivity of the measurement is higher.
[0020] The new cell absorption rate 3D testing method enhanced by a common path F-P cavity described, the cavity length of the F-P cavity is larger than the diameter of the cell to be tested 12, so it can be placed in the F-P cavity. The rotation of the cell to be tested 12 is controlled by the rotary control platform, the rotary control platform is an optical tweezers system, etc.
[0021] The new cell absorption rate 3D testing method enhanced by a common path F-P cavity, the interface reflectivity of the F-P cavity is in the range of 0 to 1.
[0022] The new cell absorption rate 3D testing method enhanced by a common path F-P cavity,
the F-P cavity has different cavity lengths, and can be in different shapes and sizes. The cavity
length of the F-P cavity is adjustable, when the cavity length is fixed, it becomes the F-P etalon.
[0023] The cell to be tested 12 may be an optically transparent biological cell. The cell to be
tested 12 is a tiny object, when the light beam passes through the cell to be tested 12, it will
produce a cylindrical lens effect, so a absorption rate matching fluid with a absorption rate
comparable to that of the outermost layer of the cell to be tested 12 needs to be selected and
filled. The cell to be tested may be biological cells with different sizes and shapes,
microorganisms, etc.
[0024] The control module 13 consists of a computer, a device control unit, and a device control
interface to control and operate the image acquirer, the rotary control platform, etc., and
complete the digital hologram recording containing cell to be tested information. After
completing the construction of the digital holographic recording optical path based on a common
path F-P cavity 11, the control module 13 is used to control the rotary control platform to rotate
for one circle carrying the cell to be tested 12, and to control the CCD to collect the digital
hologram containing information on the cell to be tested 12 and store the graph.
[0025] The computational display module 14 performs program processing of the stored digital
hologram and displays online the absorption rate information on the cell to be tested 12.
According to the new method of microscopic imaging provided by the present invention, the
digital hologram acquired by the CCD is processed to reconstruct and achieve the high
resolution 3D absorption rate distribution of the cell to be tested 12.
[0026] In the digital holographic recording optical path based on a common path F-P cavity 11,
the light beam is reflected and transmitted many times in the F-P cavity, as shown in FIG. 3b, and passes through the cell to be tested many times to record the 3D information on the cell to be tested; the cell to be tested absorbs light wave many times and the light intensity attenuation accumulates in turns, the absorption is enhanced, and finally the complex amplitude of the beam passing through the F-P cavity is as follows:
UT=U 1 R 1- Re Where, UTis the complex amplitude of the transmitted light, U is the complex amplitude of
the incident light in the F-P cavity, R is the surface reflectance of the inside of the two parallel planar glass plates of the F-P cavity, which are shown as 31 and 32 in FIG. 3a, and ' is the phase difference distribution of the cell to be tested.
[0027] The intensity distribution of the transmitted light is:
I=U * UT* (2)
[0028] Use the light intensity distribution without information on the cell to be tested, to subtract the light intensity distribution containing information on cell to be tested, resulting in an absorption light intensity distribution that contains only information on the cell to be tested,
whichis AI=IO- I. In which, Io =U.U* is the light intensity distribution without the cell to
betested, UO is the complex amplitude distribution without the cell to be tested.
[0029] The ratio of the light intensity distribution absorbed by the cell to be tested and the light intensity distribution without the cell to be tested, is the absorption rate distribution of the cell to be tested at that angle:
A = AI /10 (3)
[0030] For the common Mach-Zehnder interference-based digital holographic recording optical path, the light source is divided into two beams by a beam-splitting prism, one light beam passing through the cell to be tested acts as the objective light wave, the other light beam acts as the reference light. The two light beams are combined through the beam-splitting prism, then meets interference at the CCD recording plane to form a hologram. The beam only passes through the cell to be tested once, record the 3D information on the cell to be tested, and the cell to be tested only absorb the light intensity once. The digital hologram recorded by the CCD is numerically reconstructed, and the the complex amplitude distribution of the reproduced objective light wave is UMz. At the same time, the light intensity distribution containing the cell to be tested is obtained as follows:
'MZ =UMZUMZ* (4)
Where Uz is the complex amplitude distribution of the reproduced objective light wave.
[0031] The Mach-Zehnder interference-based digital holographic recording optical path, use the light intensity distribution without the cell to be tested to subtract the light intensity distribution with the the cell to be tested, resulting in a light intensity distribution of the absorbance of the
cell to be tested, which is AMz 'MzO-'zM.
[0032] The Mach-Zehnder interference-based digital holographic recording optical path, the light beam follows the direction of propagation and passes the cell to be tested, and the beam passes through the cell to be tested only once, the light intensity absorbed inside the cell to be tested accumulates once along the direction of the propagation. From FIG. 3b, it can be seen that in the common path F-P cavity, the incident beam is 33, the angle of incidence is 34, and the beam is reflected and transmitted in the F-P cavity many times, and passes through the cell to be measured many times. When the incident beam 33 vertically incidents, the beam is reflected and transmitted many times in the F-P cavity, passes the cell to be tested many times, and the 3D information on the cell to be tested is recorded. The cell to be tested absorbs light wave many times and the light intensity attenuation accumulates in turns, the absorption is enhanced, that is
IMz « AI. Therefore, the new cell absorption rate 3D testing method enhanced by a common path F-P cavity has a higher measurement sensitivity, and therefore the resolution is higher,
which is in principle superior to the common absorption rate testing method based on the Mach
Zehnder interference.
[0033] Therefore, in the digital holographic recording optical path based on a common path F-P
cavity 11, first record the digital hologram without placing the cell to be tested 12, then place the
cell to be tested 12 in the F-P cavity. When 0=0, the optimal digital hologram containing
information on the sample of cell to be tested 12 is recorded, and the complex amplitude
distribution Ui of the transmitted light is gained by numerically reconstructing the recorded
digital hologram. The complex amplitude distribution of the transmitted light is multiplied with
its conjugate to retrieve the light intensity distribution of the transmitted light. Use the light
intensity distribution 1J without information on the sample of cell to be tested 12, to subtract
the light intensity distribution In containing information on the sample of cell to be tested 12,
resulting in a absorption light intensity distribution AI that contains only information on the
sample of cell to be tested 12, and from A. = Al / I the absorption rate distribution A, at this
angle can be achieved. Rotate the sample of cell to be tested 12, record the optimal digital
hologram at different angles, and sequentially obtain the absorption rate distribution containing
only information on the sample of cell to be tested 12 at that angle. The high-resolution 3D
absorption rate distribution A of the sample of cell to be tested can be reconstructed by
performing an iRadon transformation of the absorption rate distribution at all angles.
[0034] The new cell absorption rate 3D testing method enhanced by a common path F-P cavity
proposed comprises the following steps:
[0035] Step 1: Record the digital hologram of the F-P cavity without the cell to be tested placed
in it. Then place the cell to be tested in the F-P cavity.
[0036] Step 2: The control module controls the rotary control platform to rotate the cell to be
tested, record the optimal digital hologram at the angel after putting in the cell to be tested, and
intercept a certain size.
[0037] Step 3: Perform numerical reconstruction of the recorded digital hologram, obtain the
complex amplitude U, of the transmitted light at that angle.
[0038] Step 4: Use the light intensity distribution 1J obtained without the cell to be tested, to
subtract the light intensity distribution I, containing the cell to be tested, resulting in a light
intensity distribution AI which only contains information on the cell to be tested at that angle.
[0039] Step 5: According to the equationA= Al / I to obtain the absorption rate distribution
of the cell to be tested at that angle.
[0040] Step 6: The control module controls the rotary control platform, so that the cell to be
tested rotates for a full circle, in turn, repeat Step 2 to Step 5, and the absorption rate distribution
of the cell to be tested at different angles can be obtained.
[0041] Step 7: By performing the computational display module, the absorption rate distribution
on each cross-section of the cell to be tested at all angles will be iRadon transformed, and this
can display online the high-resolution 3D absorption rate distribution A(x,y,z) ofthecelltobe
tested.
[0042] The new cell absorption rate 3D testing method enhanced by a common path F-P cavity
provided by the present invention, comprises an F-P cavity-based digital hologram recording,
numerical reconstruction, error processing, and a 3D absorption rate distribution reconstruction.
Compared to the previous technology, the measurement sensitivity is higher due to the use of the
digital holographic recording optical path based on a common path F-P cavity, where the light
beam is reflected multiple times in the F-P cavity and passes through the cell to be tested many
times. This new cell absorption rate 3D testing method enhanced by a common path F-P cavity
has the advantages of simple structure, high sensitivity, and higher resolution.
[0043] FIG. 1 is a schematic diagram of the structure of the new cell absorption rate 3D testing
method enhanced by a common path F-P cavity. It includes an F-P cavity-based digital hologram
recording, numerical reconstruction, error processing, and a 3D absorption rate distribution
reconstruction.
[0044] FIG. 2a is the measurement system structural diagram suitable for using the new cell
absorption rate 3D testing method enhanced by a common path F-P cavity proposed by the
present invention. Including a digital holographic recording optical path based on a common
path F-P cavity 11, a cell to be tested 12, a control module 13 and a computational display
module 14. FIG. 2b is a schematic diagram of the digital holographic recording optical path
based on a common path F-P cavity in the embodiment of present invention. In the embodiment,
the digital holographic recording optical path based on the F-P cavity includes a light source 21,
an attenuator 22, a beam expander 23, an F-P etalon 24, a cell to be tested 25, a microscopy 26, a
CCD 27, and a computer 28.
[0045] FIG. 3a is a schematic diagram of the F-P cavity in the present invention. The F-P cavity is composed of two parallel planar glass plates, and a dielectric film of an R reflectance is coated on the inward sides 31 and 32 of the two planar glass plates. FIG. 3b is a schematic diagram of a light beam incident into the F-P cavity and is reflected and transmitted multiple times in the F-P cavity. 33 is the incident light wave, 34 is the angle of incidence, 35, 37 and 39 are diagrams of the transmitted light, and 36 and 38 are diagrams of the reflected light.
[0046] FIG. 4 is a schematic diagram of the F-P cavity and the cell to be tested in the embodiment of present invention. The rotary control platform 41 controls the rotation of the cell to be tested 25, which is inserted into a cuvette 42 filled with a absorption rate matching fluid, whose absorption rate is comparable to that of the outermost layer of the cell to be tested 25, and the direction of the propagation of the light beam is shown as 43.
[0047] FIG. 5 is a step flow chart of the new cell absorption rate 3D testing method enhanced by a common path F-P cavity.
[0048] The invention is further described below in relation to specific embodiment.
[0049] Embodiment 1:
[0050] FIG. 2b provides an embodiment of a digital holographic recording optical path based on a common path F-P cavity. The optical path includes a light source 21, an attenuator 22, a beam expander 23, an F-P etalon 24, a cell to be tested 25, a microscopy 26, a CCD 27, and a computer 28. The light beam starts from the light source 21, passes the attenuator 22, and the beam energy is reduced. After the light beam passing through the beam expander 23, the diameter of the beam is enlarged, and when the light beam passes the F-P etalon 24 containing the cell to be tested 25, it is reflected many times in the F-P etalon 24 and passes the cell to be tested 25 many times.
After each transmitted beam of light from the F-P etalon 24 passing through the microscopy 26,
they are interfered with and superimposed on the CCD 27, and a digital hologram interfered is
recorded by the CCD 27 is stored in the computer 28.
[0051] In this embodiment, preferably, the light source 21 is a laser light source with a
wavelength of 532 nm, and a beam expander 23 of the corresponding wavelength is selected, an
adjustable attenuator 22 is selected, an F-P etalon 24 with a 60 mm cavity length is selected, a
microscopy 26 with a magnification of 20x is selected, and a CCD 27 with a pixel size of 3.45
p mx3.45 pm and other devices are selected.
[0052] In this embodiment, it is preferred that the F-P etalon 24, as shown in FIG. 3a, uses two
parallel planar glass plates with a 0.9 reflectance dielectric film coated on the inner surfaces to
form a 60 mm cavity length F-P etalon 24. Preferably, the cell to be tested 25 is a paramecium,
and it is placed in a cuvette 42 filled with a refractive index matching fluid, whose refractive
index is comparable to that of the outermost layer of the paramecium. Put the cuvette 42 into the
cavity of the F-P etalon 24, as shown in FIG. 4, and adjust the digital holographic recording
optical path so that the optical axis of the light beam 43 passes through the cell to be tested 25.
[0053] In this embodiment, preferably, an optical-tweezers system is selected as the rotary
control platform 41, and a control module is used to precisely control the rotation of the cell to
be tested 25 to rotate around the optical fiber axial direction for a full circle.
[0054] In this embodiment, the digital holographic recording optical path based on a common
path F-P cavity includes a light source 21, an attenuator 22, a beam expander 23, an F-P etalon
24, a microscopy 26, a CCD 27, and etc. The cell to be tested 25 is a paramecium. The control
module 3 consists of a computer 28, a device control unit and a device control interface, which
control and operate the CCD 27 and the rotary control platform 41 that controls the rotation of
the cell to be tested 25; and finishes the digital hologram recording containing information on the
microstructure optical fiber 25. The computational display module 4 programs the recorded
digital holograms according to the microscopic imaging method proposed by the invention, and
displays online the 3D absorption rate information on the cell to be tested.
[0055] This embodiment uses the new cell absorption rate 3D testing method enhanced by a
common path F-P cavity, processes the digital hologram of the cell to be tested 25, and displays
online the 3D absorption rate information on the cell to be teste 25, as shown in FIG. 5, contains
the following steps:
[0056] Step 151: Record the digital hologram of the F-P etalon 24 without the cell to be tested
placed in it. Then place the cell to be tested 25 in the F-P etalon 24.
[0057] Step 2 52: The control module controls the optical-tweezers system to rotate the cell to be
tested 25 around the axis, record the optimal digital hologram at the angel after putting in the cell
to be tested 25, and intercept a certain size of the optimal digital hologram.
[0058] Step 3 53: Perform numerical reconstruction of the recorded digital hologram.
[0059] Step 4 54: Obtain the light intensity distribution containing information on the sample of
cell to be tested 25 at that angle according to Eq. (2).
[0060] Step 5 55: Use the light intensity distribution 1J obtained without the cell to be tested
, to subtract the light intensity distribution I, containing the cell to be tested 25, resulting in
a light intensity distribution Al only contains information on the cell to be tested 25 at that angle.
[0061] Step 6 56: According to the Eq. (3) to obtain the absorption rate distribution of the cell to be tested 25 at that angle.
[0062] Step 7 57: The control module controls the optical-tweezers system, rotate once for every 1 degree, so that the cell to be tested 25 is rotated for a full circle, in turn, repeat Step 2 52 to Step 6 56, then the absorption rate distribution of the cell to be tested 25 at different angles can be obtained.
[0063] Step 8 58: By performing the computational display module 4, the absorption rate distribution on each cross-section of the cell to be tested 25 at all angles will be iRadon transformed, and this can display online the reconstructed high-resolution 3D absorption rate
distribution A(x,y,z) of the cell to be tested25.
[0064] The foregoing is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art who makes various alterations and variations to the invention in accordance with the spirit and scope of the invention shall be included in the protection of the claims of the invention.
Claims (5)
1. A new cell absorption rate three-dimensional (3D) testing method enhanced by a common
path F-P cavity. It includes an F-P cavity-based digital hologram recording, numerical
reconstruction, error processing, and a 3D absorption rate distribution reconstruction. Record the
digital hologram without the sample of cell to be tested, then place the cell to be tested in the F-P
cavity. When 0=0, record the optimal digital hologram containing information on the sample of
cell to be tested, and the recorded digital hologram is numerically reconstructed to obtain the
complex amplitude distribution U, of the transmitted light. The complex amplitude
distribution of the transmitted light is multiplied with its conjugate to retrieve the light intensity
distribution of the transmitted light. Use the light intensity distribution 1J without information
on the sample of cell to be tested, to subtract the light intensity distribution IT containing
information on sample of cell to be tested, resulting in a absorption light intensity distribution
A that contains only information on the sample of cell to be tested, and from the derived
equation the absorption rate distribution A, at this angle can be achieved. Rotate the sample of
cell to be tested, record the optimal digital hologram at different angles, and sequentially obtain
the absorption rate distribution containing only information on the sample of cell to be tested at
that angle. The high-resolution 3D absorption rate distribution A of the sample of cell to be
tested can be reconstructed by performing an iRadon transformation of the absorption rate
distribution at all angles.
2. A new cell absorption rate 3D testing method enhanced by a common path F-P cavity. It
is characterized by a measurement system which consists of a digital holographic recording
optical path based on a common path F-P cavity, a cell to be tested, a control module, and a
computational display module. The digital holographic recording optical path based on a
common path F-P cavity includes a light source, a beam expander, an F-P cavity, a microscopy,
an image acquire, and the like, all devices have a common path. The cell to be tested can be
biological cells or tiny objects. The control module consists of a computer, a device control unit and a device control interface, to control and operate the image acquirer and the rotary control platform, and complete the digital hologram recording containing information on the cell to be tested. The computational display module performs program processing on the recorded digital hologram and displays online the information on the 3D absorption rate of the cell to be tested.
3. As claimed in claim 2, a new cell absorption rate 3D testing method enhanced by a
common path F-P cavity, the characteristics of the F-P cavity also includes: (1) The cell to be
tested is located in the F-P cavity in the digital holographic recording optical path 1. (2) The
cavity length of the F-P cavity is larger than the diameter of the cell to be tested, and the
interface reflectivity of the F-P cavity is in the range of 0 to 1. (3) The F-P cavity can have
different cavity lengths.
4. As claimed in claim 1, a new cell absorption rate 3D testing method enhanced by a
common path F-P cavity, the cell to be tested may be biological cells with different sizes and
shapes, microorganisms, etc.
5. As claimed in claim 1, a new cell absorption rate 3D testing method enhanced by a
common path F-P cavity, comprising primarily the steps of:
Step 1: Record the digital hologram of the F-P cavity without the cell to be tested placed
in it. Then place the cell to be tested in the F-P cavity.
Step 2: The control module controls the rotary control platform to rotate the cell to be
tested, record the optimal digital hologram at the angel after putting in the cell to be
tested, and intercept a certain size.
Step 3: Perform numerical reconstruction of the recorded digital hologram, obtain the
phase distribution U, of the transmitted light at that angle.
Step 4: Use the light intensity distribution 1J obtained without the cell to be tested, to
subtract the light intensity distribution I, containing the cell to be tested, resulting in a light intensity distribution A which only contains information on the cell to be tested at that angle.
Step 5: According to the equation A I= / I, to obtain the absorption rate distribution
of the cell to be tested at that angle. Step 6: The control module controls the rotary control platform, so that the cell to be tested rotates for a full circle, in turn, repeat Step 2 to Step 5, and the absorption rate distribution of the cell to be tested at different angles can be obtained. Step 7: By performing the computational display module, the absorption rate distribution on each cross-section of the cell to be tested at all angles will be iRadon transformed, and
this can display online the high-resolution 3D absorption rate distribution A(x,y,z) of
the cell to be tested.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2020103535A AU2020103535A4 (en) | 2020-11-19 | 2020-11-19 | A new cell absorption rate three-dimensional (3D) testing method enhanced by a common path F-P cavity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2020103535A AU2020103535A4 (en) | 2020-11-19 | 2020-11-19 | A new cell absorption rate three-dimensional (3D) testing method enhanced by a common path F-P cavity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2020103535A4 true AU2020103535A4 (en) | 2021-01-28 |
Family
ID=74192152
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020103535A Ceased AU2020103535A4 (en) | 2020-11-19 | 2020-11-19 | A new cell absorption rate three-dimensional (3D) testing method enhanced by a common path F-P cavity |
Country Status (1)
| Country | Link |
|---|---|
| AU (1) | AU2020103535A4 (en) |
-
2020
- 2020-11-19 AU AU2020103535A patent/AU2020103535A4/en not_active Ceased
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106123770B (en) | A kind of method that refractive index dynamically measures simultaneously with pattern | |
| CN112525104B (en) | Digital holographic three-dimensional shape measuring device and method | |
| CN109990709B (en) | Method and device for measuring morphology profile parameters of bilateral dislocation differential confocal interference target pill | |
| CN101672628A (en) | Aspheric optical element surface shape detection device | |
| Brito et al. | Photorefractive digital holographic microscopy applied in microstructures analysis | |
| De Angelis et al. | Digital-holography refractive-index-profile measurement of phase gratings | |
| AU2020103535A4 (en) | A new cell absorption rate three-dimensional (3D) testing method enhanced by a common path F-P cavity | |
| CN111208089B (en) | Device and method for measuring defects in long-distance rough end face crystal body | |
| CN111289479B (en) | Phase defect detection device and method based on nonlinear thermography inversion | |
| Zhao et al. | Single-pixel phase microscopy without 4f system | |
| CN111122446A (en) | Novel three-dimensional test method for phase-enhanced cell absorption rate of common-path F-P cavity | |
| AU2020103534A4 (en) | A testing method for high-resolution three-dimensional (3D) refractive index of microstructured optical fiber | |
| Almoro et al. | Object wave reconstruction by speckle illumination and phase retrieval | |
| AU2020103536A4 (en) | A new phase-shifting phase micro imaging method based on an F-P cavity | |
| CN110987871A (en) | Novel multi-wavelength phase microscopic imaging method based on F-P cavity | |
| CN105159043A (en) | Reflective digital holographic microscopic imaging device based on telecentric optical structure | |
| CN115523865A (en) | Three-dimensional measuring device and measuring method for laser damage of optical element | |
| Pan et al. | Microtomography of the polarization-maintaining fiber by digital holography | |
| CN103884681B (en) | A kind of phase microscope formation method based on SHWS | |
| Haeberlé et al. | An introduction to diffractive tomographic microscopy | |
| CN111198169A (en) | Microstructure optical fiber high resolution three-dimensional refractive index testing method | |
| CN117705001B (en) | Digital holographic object morphology measuring device and method based on concave mirror | |
| CN114858751B (en) | Method for measuring complex refractive index of two-dimensional material | |
| CN114397089B (en) | Lens testing method based on wave surface interference information | |
| CN113418470B (en) | Spectrum scanning confocal single-exposure digital holographic measurement system and measurement method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) | ||
| MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |