AU2020103030A4 - Application of mixed fertilization of liquid bio-organic fertilizer and nitrogen fertilizer in improving yield and quality of hot peppers - Google Patents
Application of mixed fertilization of liquid bio-organic fertilizer and nitrogen fertilizer in improving yield and quality of hot peppers Download PDFInfo
- Publication number
- AU2020103030A4 AU2020103030A4 AU2020103030A AU2020103030A AU2020103030A4 AU 2020103030 A4 AU2020103030 A4 AU 2020103030A4 AU 2020103030 A AU2020103030 A AU 2020103030A AU 2020103030 A AU2020103030 A AU 2020103030A AU 2020103030 A4 AU2020103030 A4 AU 2020103030A4
- Authority
- AU
- Australia
- Prior art keywords
- fertilizer
- organic fertilizer
- application
- quality
- liquid bio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/005—Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/80—Separation, elimination or disposal of harmful substances during the treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/10—Bacillus licheniformis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/11—Bacillus megaterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
Austracy
The present invention discloses an application of mixed fertilization of a liquid
bio-organic fertilizer and a nitrogen fertilizer in improving yield and quality of hot
peppers, and belongs to the technical field of fertilizers. The liquid bio-organic
fertilizer disclosed in the present invention is a green and environment-friendly
organic fertilizer, and fully utilizes animal blood to change waste into treasure. The
application of the fertilizer can increase soil organic matter and available nitrogen,
phosphorus and potassium, enhance soil fertility and enhance disease resistance,
insect resistance and stress resistance of crops. When application of the nitrogen
fertilizer is decreased by 40% and the liquid bio-organic fertilizer is applied by 28
kg/667m2, the yield of the hot peppers can be significantly increased; meanwhile,
sensory quality and nutritional quality of the hot peppers can be improved; and
during field or protective area cultivation, mixed application of moderate amount of
the liquid bio-organic fertilizer and the nitrogen fertilizer may realize high-quality
synergistic cultivation of outdoor vegetables.
1
Description
Austracy
The present invention discloses an application of mixed fertilization of a liquid bio-organic fertilizer and a nitrogen fertilizer in improving yield and quality of hot peppers, and belongs to the technical field of fertilizers. The liquid bio-organic fertilizer disclosed in the present invention is a green and environment-friendly organic fertilizer, and fully utilizes animal blood to change waste into treasure. The application of the fertilizer can increase soil organic matter and available nitrogen, phosphorus and potassium, enhance soil fertility and enhance disease resistance, insect resistance and stress resistance of crops. When application of the nitrogen fertilizer is decreased by 40% and the liquid bio-organic fertilizer is applied by 28 kg/667m2 , the yield of the hot peppers can be significantly increased; meanwhile, sensory quality and nutritional quality of the hot peppers can be improved; and during field or protective area cultivation, mixed application of moderate amount of the liquid bio-organic fertilizer and the nitrogen fertilizer may realize high-quality synergistic cultivation of outdoor vegetables.
Description
Technical Field
The present invention relates to the technical field of fertilizers, and particularly relates to an application of mixed fertilization of a liquid bio-organic fertilizer and a nitrogen fertilizer in improving yield and quality of hot peppers.
Background
As a novel green and pollution-free fertilizer, a bio-organic fertilizer changes insoluble nutrients in soil into available nutrients through vital activities of microbes in the fertilizer, and has significant effects of fertilizing soil, increasing resistance of crops, promoting growth and development of plants and improving quality of fruits. As a seasoning, the hot pepper is popular with consumers, but the yield and quality of the hot pepper can be further improved.
Therefore, providing an application of mixed fertilization of a liquid bio-organic fertilizer and a nitrogen fertilizer in improving yield and quality of hot peppers is a problem that urgently needs to be solved by those skilled in the art.
Summary
In view of this, the present invention provides an application of mixed fertilization of a liquid bio-organic fertilizer and a nitrogen fertilizer in improving yield and quality of hot peppers.
To achieve the above purpose, the present invention adopts the following technical solutions:
Description
The present invention provides the application of the mixed fertilization of the liquid bio-organic fertilizer and the nitrogen fertilizer in improving yield and quality of hot peppers.
Further, in the application of the mixed fertilization of the liquid bio-organic fertilizer and the nitrogen fertilizer in improving yield and quality of hot peppers, the nitrogen fertilizer is replaced with 40% of the liquid bio-organic fertilizer to be applied.
Further, a preparation method of the liquid bio-organic fertilizer includes:
(1) sterilizing animal blood at 60±5°C;
(2) adding 10 g of saccharomycetes and 1-2 kg of sucrose into 15-20 kg of water for culture at 40±2°C; adding the solution into 1 ton of animal blood; mixing the obtained mixed solution with 0.5-1.0 kg of protease; and performing enzymolysis for 4-6 hours;
(3) cooling the solution to 36±2°C; adding a beneficial bacterium mixed solution containing Bacillus megaterium, Bacillus licheniformis, Bacillus coagulans, lactic acid bacteria and EM bacteria; stirring the mixed solution; and performing fermentation for 10-12 hours;
(4) cooling the solution to 30±2°C; and performing anaerobic fermentation for 13-15 hours;
(5) sampling and measuring viable count of more than 200 million CFU/mL; measuring a pH value of 5-6; cooling the solution to 20°C or a room temperature; and filling the solution.
The animal blood is waste blood of animals slaughtered in a slaughter house.
Through the above technical solutions, compared with the prior art, the present invention discloses the application of the mixed fertilization of the liquid bio-organic fertilizer and the nitrogen fertilizer in improving yield and quality of hot peppers. The liquid bio-organic fertilizer is a green and environment-friendly
Description
organic fertilizer, and fully utilizes the animal blood to change waste into treasure. The application of the fertilizer can increase soil organic matter and available nitrogen, phosphorus and potassium, increase soil fertility and enhance disease resistance, insect resistance and stress resistance of crops. Meanwhile, field experiments show that, the liquid bio-organic fertilizer has excellent economic benefits and market application prospects. When application of the nitrogen fertilizer is decreased by 40% and the liquid bio-organic fertilizer is applied by 28 kg/667m2, the yield of the hot peppers can be significantly increased, and a fruit shape index, soluble protein content, soluble sugar content, VC content and nitrate content are respectively 6.07, 3.25 mg/g, 59.96%, 370.37 mg/100 g and 318.56 mg/kg. Meanwhile, sensory quality and nutritional quality of the hot pepper can be improved; and during field or protective area cultivation, mixed application of moderate amount of the liquid bio-organic fertilizer and the nitrogen fertilizer may realize high-quality synergistic cultivation of outdoor vegetables.
Detailed Description
The technical solution in embodiments of the present invention will be clearly and fully described below. Apparently, the described embodiments are merely part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those ordinary skilled in the art without contributing creative labor will belong to the protection scope of the present invention. Embodiment 1
A preparation method of a liquid bio-organic fertilizer includes the following specific steps:
(1) animal blood was sterilized at 60±5°C;
(2) 10 g of saccharomycetes and 2 kg of sucrose were added into 20 kg of water for culture at 40±2°C; the solution was added into 1 ton of animal blood; the
Description
obtained mixed solution was mixed with 1.0 kg of protease; and enzymolysis was performed for 6 hours;
(3) the solution was cooled to 36±2°C; a beneficial bacterium mixed solution containing Bacillus megaterium, Bacillus lichenformis, Bacillus coagulans, lactic acid bacteria and EM bacteria was added; the mixed solution was stirred; and fermentation was performed for 12 hours;
(4) the solution was cooled to 30±2°C; and anaerobic fermentation was performed for 15 hours;
(5) the solution was sampled; viable count was measured to be more than 200 million CFU/nL; a pH value was measured to be 5-6; the solution was cooled to °C or a room temperature; and the solution was filled.
Influences of the liquid bio-organic fertilizer prepared in embodiment 1 that replaces the nitrogen fertilizer on the yield and quality of hot peppers are tested. (1) Overview of a test area and materials
A test was conducted in a practice base of Sanping Farm in Xinjiang Agricultural University in May to October in 2019. Tested hot pepper was Huiteng #8. A test fertilizer was a common commercially available chemical fertilizer, i.e., urea (including 46% of N), calcium superphosphate (including 12% of P 2 05 ) and potassium sulfate (including 51% of K2 0). A test bio-organic fertilizer is the liquid bio-organic fertilizer prepared in embodiment 1 of the present invention, and includes >2% of trace elements, 100 mg/kg of amino acid content, >200 million of viable count, >30% of organic matter content, 6% of nitrogen content, 4% of P 2 05 and 2% of K20. Basic physical and chemical properties of soil of a test field were as follows: a pH value of 8.04, total salt of 0.80 g/kg, total nitrogen of 0.97 g/kg, organic matter of 20.53 g/kg, available nitrogen of 76.50 mg/kg, available phosphorus of 26.90 mg/kg and available potassium of 339 mg/kg. (2) Test design
Description
6 different nitrogen levels were set up in an open field culture manner. A nitrogen fertilizer was conventionally applied in control CK; in T1, the application of the nitrogen fertilizer was decreased by 10%; in T2, the application of the nitrogen fertilizer was decreased by 20%; in T3, the application of the nitrogen fertilizer was decreased by 30%; in T4, the application of the nitrogen fertilizer was decreased by 40%; and in T5, the application of the nitrogen fertilizer was decreased by 50%. Meanwhile, the liquid bio-organic fertilizer prepared in embodiment 1 was applied for supplementing nutrient elements deficient in the growth process of plants. Application amounts of phosphorus and potash fertilizers in various treatments in the cultivation process were identical (30 kg/mu), wherein % of the nitrogen fertilizer and the potash fertilizer were basally applied respectively; in the rest 70%, 10% was applied at a first hot pepper stage, 20% was applied at a paired hot pepper stage, 30% was applied at a full bearing stage and % was subjected to topdressing at a final fruiting stage. The phosphorus fertilizer was completely basally applied. Protective row plants were arranged among plots; a spacing between two rows was 35 cm x50 cm; and two plants were cultivated in each hole. Amounts of the nitrogen fertilizer and the liquid bio-organic fertilizer in each treatment were shown in Table 1. Table 1 Application amounts in different treatments Urea (kg/667m 2 ) Liquid Amount of basic Amount of bio-organic Treatment fertilizer topdressing fertilizer (kg/667m 2 )
CK 10.17 23.73 0.00 TI 9.15 21.36 22.00 T2 8.14 18.98 24.00 T3 7.12 16.61 26.00 T4 6.10 14.24 28.00 T5 5.09 11.86 30.00
Test data were processed by Excel 2010, and variance analysis was conducted on the data by using SPSS19.0 software. (3) Growth traits
Description
At the full bearing stage of the hot pepper (on July 26), 10 plants of the hot pepper were randomly selected from each plot. Plant heights (heights from stem base parts to growth points of the hot pepper), stem diameters (diameters of parts that are 1 cm above the stem base parts), plant crown diameters (maximum diameters of vertical projections of leaf curtains), leaf numbers and SPAD values (3 upper, middle and lower leaves of each plant of the hot pepper were selected and relative chlorophyll content was tested by using an SPAD-502 instrument) were determined. Results were shown in Table 2. Table 2 Changes of growth indexes of hot pepper in different treatments Treatment Plant height Stem Plant crown Leaf Relative (cm) (M) diameter diameters number chlorophyll (cm) (cm) (piece) content (SPAD value) CK 83.48c 13.25b 54.71c 190.44c 62.30c TI 87.71a 13.30b 58.62ab 245.44ab 62.67c T2 88.52a 13.88a 60.24a 235.78b 64.69abc T3 87.43a 14.09a 58.00ab 251.30a 66.99a T4 88.24a 13.84a 58.62ab 245.1lab 63.64bc T5 85.48b 13.91a 57.38b 254.56a 65.54ab
Notes: different lowercase letters behind data of the same column represent significant differences (P<0.05), the same below.
It can be seen from Table 2 that, the plant heights, stem diameters, plant crown diameters, leaf numbers and relative chlorophyll content in various treatments were all higher than those in the CK. Plant heights in T2 and T4 were respectively up to 88.52 cm and 88.24 cm, having significant differences from those in the CK; the stem diameter in T3 was the largest, up to 14.09 mm, and was 6.34% higher than that in the CK; the plant crown diameter in the T2 was the largest and was up to 60.24 cm; the order of leaf numbers from large to small was T5>T3>T1> T4>T2>CK, and T5 had the greatest leaf number and had significant difference from the CK; and T3 had the highest relative chlorophyll content up to 66.99, having significant difference from that in the CK. (4) Yield
Description
At the mature stage of the hot pepper, 20 plants of the hot pepper were randomly selected from each test plot for testing weight per fruit, fruit number per plant, yield per plant, plot yield and yield per unit. Results were shown in Table 3. Table 3 Changes of yield indexes of hot pepper in different treatments Trea Weight per Fruit Yield per Plot yield Yield of fresh hot tmen fruit(g) number plant (g) (kg/36m 2) pepper t per (kg/667m 2
) plant (piece) CK 63.68a 29.33c 1143.93b 151.00b 2797.68b TI 51.35d 38.17a 954.65c 126.01c 2334.76c T2 60.79b 28.50c 916.94c 121.04c 2242.53c T3 54.92c 33.00b 1257.20b 169.72ab 3144.57ab T4 63.79a 37.83a 1259.62b 188.21a 3487.13a T5 55.76c 40.67a 1484.90a 179.94a 3185.62a
It can be seen from Table 3 that, with decrease of nitrogen application and increase of the liquid bio-organic fertilizer, the yield per plant had the trend of decreasing first and then increasing. T4 had the largest weight per fruit up to 63.69 g; weights per fruit of other treatments were all lower than those in the CK; TI had the smallest weight per fruit, up to 51.35 g; T5 had the largest fruit number per plant, that is, 40.67 fruits; T5 had the largest yield per plant, up to 1484.90 g, and the yield was increased by 29.81% compared with that of the CK and was greatly 2 higher than the yield in TI and T2; T4 and T5 had higher plot yields of 188.21m and 179.94 kg/36m 2 respectively, being extremely significantly higher than the CK; and the yield of fresh hot pepper in the treatment T4 was the largest, was up to 3487.13 kg/667m 2, and was increased by 24.64% compared with that of the CK. (5) Quality traits
At the mature stage of first hot pepper, 15 pieces of the hot pepper were randomly selected from each test plot for testing longitudinal diameters, transverse diameters, pulp thicknesses and fruit hardness of the hot pepper; and a fruit shape index (fruit shape index=longitudinal diameter/transverse diameter) was calculated. Results were shown in Table 4.
Description
Table 4 Changes of sensory quality indexes of hot pepper in different treatments treatment Longitudinal Transverse Fruit shape Pulp Fruit diameter diameter index thickness hardness (cm) (mm) (mm) CK 18.82ab 35.24a 5.39a 4.04a 43.80ab TI 17.39b 31.84b 5.60a 3.51b 44.20ab T2 19.15a 34.22ab 5.68a 3.63b 40.73c T3 18.69ab 34.22ab 5.49a 3.53b 42.13bc T4 19.16a 32.04b 6.07a 3.65b 45.13a T5 17.63ab 32.46ab 5.69a 3.40b 41.47bc
It can be seen from Table 4 that, mixed application of the liquid bio-organic fertilizer may significantly increase the fruit shape index of the hot pepper. The fruit shape indexes in the various treatments were respectively increased by 3.90%, 5.38%, 1.86%, 12.62% and 5.57% compared with that in the CK; the longitudinal diameter of fruits at T4 was the longest, that is, 19.16 cm, and was increased by 1.81% compared with that in the CK; the transverse diameter of fruits at T4 was 32.04 mm, which was decreased by 9.08% compared with that in the CK; the pulp thickness of the hot pepper in each treatment was lower than that in the CK, which indicated that the decreased application of the nitrogen fertilizer and mixed application of the liquid bio-organic fertilizer can inhibit the pulp thickness of the hot pepper; and the treatment T4 had the largest fruit hardness of 45.13 that had no significant difference from that in the CK.
At the mature stage of the paired hot pepper, 9 pieces of the hot pepper were randomly selected from each test plot to test hot pepper quality. Soluble proteins were determined by a Coomassie brilliant blue G-250 dyeing method; soluble sugar was determined by an anthrone-sulfuric acid colorimetic method; VC was determined by a 2,6-dichloroindophenol titration method; and nitrate was tested by a salicylic acid colorimetic method. Results were shown in Table 5. Table 5 Change of nutrient quality indexes of hot pepper in different treatments Treat Soluble protein Soluble VC (mg/100g) Nitrate ment (mg/g) sugar (%) (mg/kg) CK 2.93ab 57.86a 283.93c 411.62a
Description
TI 2.73bc 55.86ab 321.13bc 345.20c T2 2.88ab 59.47a 341.40b 387.72ab T3 2.86ab 52.59b 363.80b 356.59bc T4 3.25a 59.96a 370.37ab 318.56c T5 2.44c 42.53c 421.63a 236.44d
It can be seen from Table 5 that, along with the decrease of nitrogen application and increase of the liquid bio-organic fertilizer, VC had an increasing trend. Soluble proteins of the hot pepper in the treatment T4 were remarkably increased compared with those in the CK, that is, increased by 10.92% compared with the CK; soluble sugar content of the hot pepper in the treatment T4 was up to 59.95%, and was increased by 3.63% compared with that in the CK; VC contents in the various treatments were respectively increased by 13.10%, 20.24%, 28.13%, 30.44% and 48.50% compared with that in the CK; T5 had the highest VC content; mixed application of the liquid bio-organic fertilizer may decrease the content of nitrate in fruits of the hot pepper; nitrate contents in T4 and T5 were respectively decreased by 22.61% and 42.56% compared with that in the CK; and nitrate contents in different fertilization treatment were all lower than standard allowable nitrate content (432 mg/kg) of solanaceous vegetables in China.
Quality indexes of the hot pepper were evaluated by using membership functions; a formula U(X)=(X-Xmin)/(Xmax-Xmin) was used; an inverse membership function formula U'(X)=1-(X-Xmin)/(Xmax-X min) was used; and in the formula, Xi was a measured index value; and Xmax and Xmin were the maximum value and the minimum value of a certain index of all tested materials. Results were shown in Table 6. Table 6 Membership function of quality of hot pepper Treatment U(1) U(2) U(3) U(4) U(5) U(6) X CK 0.00 1.00 0.60 0.88 0.00 0.00 0.414 TI 0.31 0.17 0.36 0.76 0.27 0.38 0.375 T2 0.43 0.36 0.54 0.97 0.42 0.14 0.476 T3 0.15 0.20 0.52 0.58 0.58 0.31 0.390 T4 1.00 0.39 1.00 1.00 0.63 0.53 0.758 T5 0.44 0.00 0.00 0.00 1.00 1.00 0.407
Notes: U(1), U(2), U(3), U(4) and U(5) respectively represent membership function values of fruit shape index, pulp thickness, soluble protein, soluble sugar
Description
and VC; U(6) represents an inverse membership function value of nitrate content; and X represents the mean value of various index membership functions.
It can be seen from Table 6 that, the treatment T4 had the highest comprehensive evaluation, that is, 0.758; and the order of comprehensive evaluation was T4>T2>CK>T5>T3>T1.
Through comparison of the yield and quality of the hot pepper in different treatments, the yield of the hot pepper may be significantly increased in the treatment T4 (nitrogen application is decreased by 40% and the liquid bio-organic fertilizer is applied by 28 kg/667m 2). The fruit shape index, soluble protein content, soluble sugar content, VC and nitrate are respectively 6.07, 3.25 mg/g, 59.96%, 370.37 mg/100 g and 318.56 mg/kg. Meanwhile, the sensory quality and nutritional quality of the hot pepper can be improved in the T4 treatment. During field or protective area cultivation, mixed application of moderate amount of the liquid bio-organic fertilizer and the nitrogen fertilizer may realize high-quality synergistic cultivation of outdoor vegetables.
The above description of the disclosed embodiments enables those skilled in the art to realize or use the present invention. Many modifications to these embodiments will be apparent to those skilled in the art. The general principle defined herein can be realized in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principle and novel features disclosed herein.
1 )
Claims (3)
1. An application of mixed fertilization of a liquid bio-organic fertilizer and a nitrogen fertilizer in improving yield and quality of hot peppers.
2. The application of mixed fertilization of the liquid bio-organic fertilizer and the nitrogen fertilizer in improving yield and quality of hot peppers according to claim 1, wherein the nitrogen fertilizer is replaced with 40% of the liquid bio-organic fertilizer to be applied.
3. The application of mixed fertilization of the liquid bio-organic fertilizer and the nitrogen fertilizer in improving yield and quality of hot peppers according to claim 1 or 2, wherein a preparation method of the liquid bio-organic fertilizer comprises:
(1) sterilizing animal blood at 60±5°C;
(2) adding 10 g of saccharomycetes and 1-2 kg of sucrose into 15-20 kg of water for culture at 40±2°C; adding the solution into 1 ton of animal blood; mixing the obtained mixed solution with 0.5-1.0 kg of protease; and performing enzymolysis for 4-6 hours;
(3) cooling the solution to 36±2°C; adding a beneficial bacterium mixed solution containing Bacillus megaterium, Bacillus licheniformis, Bacillus coagulans, lactic acid bacteria and EM bacteria; stirring the mixed solution; and performing fermentation for 10-12 hours;
(4) cooling the solution to 30±2°C; and performing anaerobic fermentation for 13-15 hours;
(5) sampling and measuring viable count of more than 200 million CFU/mL; measuring a pH value of 5-6; cooling the solution to 20°C or a room temperature; and filling the solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2020103030A AU2020103030A4 (en) | 2020-10-27 | 2020-10-27 | Application of mixed fertilization of liquid bio-organic fertilizer and nitrogen fertilizer in improving yield and quality of hot peppers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2020103030A AU2020103030A4 (en) | 2020-10-27 | 2020-10-27 | Application of mixed fertilization of liquid bio-organic fertilizer and nitrogen fertilizer in improving yield and quality of hot peppers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2020103030A4 true AU2020103030A4 (en) | 2020-12-24 |
Family
ID=73838836
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020103030A Active AU2020103030A4 (en) | 2020-10-27 | 2020-10-27 | Application of mixed fertilization of liquid bio-organic fertilizer and nitrogen fertilizer in improving yield and quality of hot peppers |
Country Status (1)
| Country | Link |
|---|---|
| AU (1) | AU2020103030A4 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113773988A (en) * | 2021-08-27 | 2021-12-10 | 微生物肥料技术研究推广中心 | Bacillus subtilis and application thereof in disease prevention and yield increase of pepper |
-
2020
- 2020-10-27 AU AU2020103030A patent/AU2020103030A4/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113773988A (en) * | 2021-08-27 | 2021-12-10 | 微生物肥料技术研究推广中心 | Bacillus subtilis and application thereof in disease prevention and yield increase of pepper |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Rueda et al. | Effect of Azospirillum spp. and Azotobacter spp. on the growth and yield of strawberry (Fragaria vesca) in hydroponic system under different nitrogen levels | |
| CN102329754B (en) | Novel strain of streptomyces fradiae and application thereof | |
| Arumugam et al. | Response of Arbuscular mycorrhizal fungi and Rhizobium inoculation on growth and chlorophyll content of Vigna unguiculata (L) Walp Var. Pusa 151 | |
| Latt et al. | The effects of green manure (Sesbania rostrata) on the growth and yield of rice | |
| Gunarto et al. | Isolation and selection of indigenous Azospirillum spp. from a subtropical island, and effect of inoculation on growth of lowland rice under several levels of N application | |
| CN105272638A (en) | Compound fertilizer special for peanuts and preparing method of compound fertilizer | |
| CN107580818A (en) | A kind of soil conditioning and the integrated approach repaired | |
| AU2020103030A4 (en) | Application of mixed fertilization of liquid bio-organic fertilizer and nitrogen fertilizer in improving yield and quality of hot peppers | |
| Abobaker et al. | Effect of fertiliser type and mycorrhizal inoculation on growth and development of sunflower (Helianthus annuus L.) | |
| Liu et al. | Effects of microbial fertilizer and irrigation amount on growth, physiology and water use efficiency of tomato in greenhouse | |
| Menge et al. | Rhizobial inoculation methods affect the nodulation and plant growth traits of host plant genotypes: a case study of common bean Phaseolus vulgaris L. germplasms cultivated by smallholder farmers in Eastern Kenya. | |
| Raju | Study on constraints and adoption of black gram seed production technologies by the farmers of Cauvery delta zone of Tamil Nadu | |
| Ahmed et al. | Effect of nitrogen, phosphorus and potassium fertilizers on yield and yield attributes of marigold (Tagetes patula L.). | |
| Mortvedt | Nitrogen and molybdenum uptake and dry matter relationships of soybeans and forage legumes in response to applied molybdenum on acid soil | |
| CN110606781A (en) | Full-water-soluble organic-inorganic compound fertilizer and preparation method thereof | |
| Ekanayake et al. | Effect of organic and inorganic liquid fertilizers on growth and yield of Centella asiatica (L.) and Capsicum annuum (L.) var. Annuum | |
| Ezekiel-Adewoyin | Evaluation of the growth response of soybean (Glycine max L.) to some commercial fertilizers in the guinea savannah agro-ecological zone of Ghana | |
| Taha et al. | Impact of different sources of natural, mineral and bio-Fertilizers on apple trees performance, growth and yield on sandy soil | |
| Andrabi et al. | Influence of plant growth regulators on leaf and seed yield of coriander (Coriandrum sativum L.) var. Shalimar dhania-1 | |
| Singh et al. | Effect of different levels of nitrogen, phosphorus and Sulphur on growth and yield of Rajmash (Phaseolus Vulgaris L.) Variety HUR 15 | |
| CN112979383A (en) | Bio-organic fertilizer for promoting crop growth and preparation method and application thereof | |
| Bildirici | The effects of copper-zinc interactions on yield and yield components in soilless grown beans (Phaseolus vulgaris L.). | |
| Phat et al. | Influence of two phosphate-potassium solubilizing bacterial species on biomass and nitrate concentration on mustard greens (Brassica juncea (L.) Czernjaew) cultivated on acid sulfate soils | |
| RU2776942C1 (en) | Method for producing a liquid complex microbiological fertiliser | |
| CN108689779B (en) | Bioactive seed dressing agent containing spirulina and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) |