AU2020102161A4 - Self-assembled nanosphere and preparation method and application method thereof - Google Patents
Self-assembled nanosphere and preparation method and application method thereof Download PDFInfo
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- AU2020102161A4 AU2020102161A4 AU2020102161A AU2020102161A AU2020102161A4 AU 2020102161 A4 AU2020102161 A4 AU 2020102161A4 AU 2020102161 A AU2020102161 A AU 2020102161A AU 2020102161 A AU2020102161 A AU 2020102161A AU 2020102161 A4 AU2020102161 A4 AU 2020102161A4
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- 239000002077 nanosphere Substances 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 229910021389 graphene Inorganic materials 0.000 claims abstract description 43
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 41
- 229920001661 Chitosan Polymers 0.000 claims abstract description 30
- 238000011068 loading method Methods 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 11
- 150000003384 small molecules Chemical class 0.000 claims abstract description 11
- 238000013268 sustained release Methods 0.000 claims abstract description 11
- 239000012730 sustained-release form Substances 0.000 claims abstract description 11
- 230000009881 electrostatic interaction Effects 0.000 claims abstract description 6
- 239000001257 hydrogen Substances 0.000 claims abstract description 4
- 229910052739 hydrogen Inorganic materials 0.000 claims abstract description 4
- 239000011148 porous material Substances 0.000 claims abstract description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 42
- 229910052757 nitrogen Inorganic materials 0.000 claims description 21
- 239000002105 nanoparticle Substances 0.000 claims description 20
- 238000001035 drying Methods 0.000 claims description 9
- REJHVSOVQBJEBF-OWOJBTEDSA-N 5-azaniumyl-2-[(e)-2-(4-azaniumyl-2-sulfonatophenyl)ethenyl]benzenesulfonate Chemical compound OS(=O)(=O)C1=CC(N)=CC=C1\C=C\C1=CC=C(N)C=C1S(O)(=O)=O REJHVSOVQBJEBF-OWOJBTEDSA-N 0.000 claims description 8
- 239000006185 dispersion Substances 0.000 claims description 8
- REJHVSOVQBJEBF-UHFFFAOYSA-N DSD-acid Natural products OS(=O)(=O)C1=CC(N)=CC=C1C=CC1=CC=C(N)C=C1S(O)(=O)=O REJHVSOVQBJEBF-UHFFFAOYSA-N 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000012296 anti-solvent Substances 0.000 claims description 4
- 230000001678 irradiating effect Effects 0.000 claims description 3
- 239000004971 Cross linker Substances 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 238000004132 cross linking Methods 0.000 claims description 2
- 238000001879 gelation Methods 0.000 claims description 2
- 239000007970 homogeneous dispersion Substances 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000002604 ultrasonography Methods 0.000 abstract description 5
- 239000005452 food preservative Substances 0.000 abstract description 3
- 235000019249 food preservative Nutrition 0.000 abstract description 3
- 238000009920 food preservation Methods 0.000 abstract description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 21
- 230000000694 effects Effects 0.000 description 5
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 5
- 125000003277 amino group Chemical group 0.000 description 4
- 239000000839 emulsion Substances 0.000 description 4
- 238000009210 therapy by ultrasound Methods 0.000 description 4
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 description 3
- 235000016639 Syzygium aromaticum Nutrition 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 3
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 description 3
- 229960003987 melatonin Drugs 0.000 description 3
- 239000004311 natamycin Substances 0.000 description 3
- 235000010298 natamycin Nutrition 0.000 description 3
- NCXMLFZGDNKEPB-FFPOYIOWSA-N natamycin Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C[C@@H](C)OC(=O)/C=C/[C@H]2O[C@@H]2C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 NCXMLFZGDNKEPB-FFPOYIOWSA-N 0.000 description 3
- 229960003255 natamycin Drugs 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 239000000341 volatile oil Substances 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 2
- 244000223014 Syzygium aromaticum Species 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 125000004432 carbon atom Chemical group C* 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- XEYBHCRIKKKOSS-UHFFFAOYSA-N disodium;azanylidyneoxidanium;iron(2+);pentacyanide Chemical compound [Na+].[Na+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].[O+]#N XEYBHCRIKKKOSS-UHFFFAOYSA-N 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 239000002086 nanomaterial Substances 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 229940083618 sodium nitroprusside Drugs 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000061408 Eugenia caryophyllata Species 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000000227 bioadhesive Substances 0.000 description 1
- 230000007227 biological adhesion Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 229920006317 cationic polymer Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000006196 deacetylation Effects 0.000 description 1
- 238000003381 deacetylation reaction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 229920000867 polyelectrolyte Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3526—Organic compounds containing nitrogen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/015—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
- A23L29/275—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of animal origin, e.g. chitin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/358—Inorganic compounds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
- C01B32/182—Graphene
- C01B32/198—Graphene oxide
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Food Science & Technology (AREA)
- Nanotechnology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Composite Materials (AREA)
- Carbon And Carbon Compounds (AREA)
- Materials Engineering (AREA)
- Manufacturing & Machinery (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Dispersion Chemistry (AREA)
Abstract
A self-assembled nanosphere, a preparation method and an application on the sustained
release of food preservative are disclosed, relateing to the field of food preservation. The
self-assembled nanosphere includes: graphene oxide forms self-assembled spheres through
electrostatic interaction and intermolecular hydrogen bonding with chitosan after loading
small molecules; adjusting the pore size of graphene oxide by controlling ultrasound to
perform selective release; adjusting the number of graphene chitosan layers to regulate the
release rate of the loading material. The self-assembled nanospheres prepared by the present
disclosure have loading and sustained release, so as to realize the selective release of small
molecular loading material and the precise control of release rate. In addition, it can be
hydrated to form a film in the process of use, so as to enhance its application range.
Description
The present disclosure relates to the technical field of food preservation, in particular to a
self-assembled nanosphere and its preparation method and application in the sustained release
of food preservative.
BACKGROUND Graphene is a kind of monolayer planar film with hexagonal honeycomb lattice
composed of carbon atoms in sp2 hybrid orbitals, and the main chemical components are
carbon atoms and polar oxygen-containing functional groups, which is widely payed attention
as an excellent material. The specific surface area of graphene and graphene oxide is as high
as 2600m 2/g, which is about 1.5-2 times of that of conventional activated carbon, and
graphene and graphene oxide can be used as carriers of small molecules. Because of its special
structure and barrier effect, the selective release and sustained release of small molecules can
be controlled by adjusting the diameter of the internal pores.
Chitosan (CS), chemical name poly(1,4)-2-amino-2-deoxy-p-D-glucose, is natural
polysaccharide polymer obtained from a deacetylation derivative of chitin. Chitosan has the
advantages of excellent broad-spectrum antibacterial and good film-forming property, and
Chitosan is environment friendly, easy to degrade and non toxic and pollution-free. The
positive charge of chitosan in water can produce electrostatic interaction with negatively
charged graphene oxide. At the same time, the hydroxyl and carboxyl groups of porous
graphene oxide are easy to perform hydrogen-bonding interaction with the amino group of
nano chitosan, thus forming self-assembled structure. At the same time, the hydroxyl and
amino groups in chitosan molecular chain can also provide reduction stability for graphene
oxide nanomaterials. In addition, the good biocompatibility and biodegradability of chitosan
make it possible for chitosan based materials to be used in the field of biological agriculture.
SUMMARY The present disclosure is to provide a self-assembled nanosphere and its preparation
method and application in the sustained release of food preservative. The self-assembled
nanosphere has good sustained release effect on the small molecule preservative.
A self-assembled nanosphere and its preparation method and application method, characterized in that: immersing load into graphene oxide dispersion for 10 min under ultrasonic condition, and then drying with nitrogen to form nanoparticles, and then immersing the nanoparticles into chitosan solution for 10 min, then drying with nitrogen, and then repeated the above steps to prepare multilayer nanospheres; then immersing the multilayer nanospheres into 4,4'-Diaminostilbene-2,2'-disulfonic acid (DAS) solution for 30min and then taking the multilayer nanospheres out, drying with nitrogen, and irradiating by UV to prepare sustained-release self-assembled nanospheres. Preferably, the solid content of the homogeneous dispersion of the graphene oxide in water is 0.1-0.5mg/mL.
Graphene oxide can be used as material carrier because of its large specific surface area.
At the same time, its special structure has barrier effect and can control the sustained-release of small molecules. Ultrasonic treatment can increase the inner channel diameter of graphene
film, so as to selectively penetrate some small molecules. The release rate of small molecules
is controlled by adding the number of graphene oxide layers.
The chitosan ionized in water with positive charge can generate electrostatic interactions with the graphene oxide with negative charge. At the same time, the hydroxyl and carboxyl
groups of porous graphene oxide and the amino groups of nano-chitosan are prone to hydrogen bonding to form a self-assembled structure. In addition, the hydroxyl and amino
groups in the chitosan molecular chain can also provide reduction and stabilization for
graphene oxide nanomaterials.
The hydrophilic group of the chitosan makes the self-assembled nanospheres have film-forming property and biological adhesion property.
The present disclosure also provides an application method of self-assembled nanosphere,
including: Step 1: Adding the prepared loading material solution or emulsion to the graphene oxide
nanoparticle dispersion under ultrasound condition, and drying it with nitrogen to form
nanoparticles.
Step 2: Immersing the nanoparticles in the chitosan acetic acid solution, drying with nitrogen;
Step 3: Repeating Step 1 and Step 2;
Step 4: Immersing the nanospheres in 4,4'-diaminostilbene-2,2'-disulfonic acid solution
(DAS), drying with nitrogen, and irradiating with UV.
Graphene oxide can be used for loading because of its large specific surface area, and its
special structure has barrier effect, which can control the sustained-release of small molecules.
Ultrasonic treatment can increase the inner channel diameter of graphene film, so as to selectively penetrate some small molecules with a diameter of about 1 nm. The release rate of
small molecules is controlled by adding the number of graphene oxide layers.
Due to the solubility and instability of graphene, its application is limited. However, chitosan, as a natural cationic polymer polysaccharide, can form self-assembled spheres with
graphene oxide with negative charge in nano particle dispersion through intermolecular
hydrogen bonding and electrostatic interaction. The hydroxyl and amino groups in the chitosan
molecular chain can provide reduction stabilization for graphene oxide, and the hydrophilic groups of chitosan make the self-assembled nanospheres have film-forming and bioadhesive
properties.
Preferably, the preparation of loading material solution or emulsion is used the bottom-up anti-solvent precipitation method or the ionic gelation method in Step 1.
Preferably, the mass concentration of the graphene oxide is 0.1-0.5g/L due to the good
adsorption property of the graphene oxide. Preferably, since ultrasonic treatment can change the pore size of the graphene oxide, the
ultrasonic treatment time is 20 to 60 min.
Preferably, the positive charge of the chitosan acetic acid solution and the graphene oxide
with negative charge form a polyelectrolyte complex through electrostatic interaction, and the mass concentration of the chitosan acetic acid solution is 6 to 20 mg/L in Step 2.
Preferably, the immersion time of photosensitive crosslinker DAS is 15 to 45 min, and a
cross-linking reaction takes place 20 to 40 min under ultraviolet lamp irradiation, to form a covalent cross-linked network in multilayer film, so as to enhance the stability of the
nanosphere.
The advantageous effects of the present disclosure are as follows: The self-assembled nanospheres have the functions of loading and sustained-release, and can realize the selective release and the precise control of release rate of of small molecular loading material. Besides, it can be hydrated into a film during use.
DETAILED DESCRIPTION OF THE EMBODIMENTS Hereinafter, the present disclosure will be further described in detail through embodiments, the objective of which is to more specifically and clearly illustrate the content of the present disclosure without limiting the protection scope of the present disclosure. Embodiment 1 Step 1: Sodium nitroprusside is added into 0.3g/L graphene oxide nanoparticles dispersion solution, ultrasonic wave is performed for 20 minutes, and then dried with nitrogen, so as to prepare the nanoparticles; Step 2: The nanoparticles are immersed in 30 mg/L chitosan acetic acid solution for 10 min, and then dried with nitrogen; Step 3: Repeat Step 1 and Step 2 for 10 times; Step 4: The nanospheres are immersed in 4,4'-diaminostilbene-2,2'-disulfonic acid solution (DAS) for 20 min, dried with nitrogen, and irradiated by UV for 30 min; Step 5: The strawberries are placed in a closed container at 1 for storage; sodium nitroprusside self-assembled nanospheres are put into nylon bag and suspended above the container to release NO and inhibit the occurrence of chilling injury. Embodiment 2 Step 1: Clove essential oil emulsion is prepared by the ionic gel method; Step 2: The emulsion obtained in step 1 is added to the 0.5g/L graphene oxide nanoparticle dispersions, ultrasound is performed for 30min, and then dried with nitrogen to prepare nanoparticles; Step 3: The nanoparticles are immersed in 20 mg/L chitosan acetic acid solution for 10 min, and then dried with nitrogen; Step 4: Repeat step 1 and step 2 for 5 times; Step 5: The nanospheres are immersed in 4,4'-diaminostilbene-2,2'-disulfonic acid solution (DAS) for 15 min, dried with nitrogen, and irradiated by UV for 20 min; Step 6: the grapes are placed in the sealed container, and the clove essential oil self-assembled nanospheres are put into nylon cloth bag and suspended above the container to release clove essential oil slowly and inhibit bacteria. Embodiment 3 Step 1: Melatonin nanoparticles are prepared by a bottom-up anti-solvent precipitation method; Step 2: The melatonin nanoparticles obtained in step 1 are added into 0.4g/LA graphene oxide dispersion solution, ultrasound is performed for 20 minutes, and dried with nitrogen to prepare curcumin-graphene nanospheres; Step 3: The curcumin-graphene nanoparticles are immersed in 20 mg/L chitosan acetic acid solution for 10 min, and then dried with nitrogen; Step 4: Repeat step 1 and step 2 for 5 times Step 5: The nanospheres are immersed in 4,4'-diaminostilbene-2,2'-disulfonic acid solution (DAS) for 30 min, dried with nitrogen, and irradiated by UV for 30 min. Step 6: The prepared melatonin self-assembled nanospheres are hydrated to form an anti-oxidation film and preservative film for increasing plant stress resistance. Embodiment 4 Step 1: Natamycin nanoparticles are prepared by a bottom-up anti-solvent precipitation method; Step 2: 0.5g/L graphene oxide dispersion is added into natamycin nanoparticles obtained in step 1, ultrasound is performed for 20 minutes, and then dried with nitrogen to prepare curcumin-graphene nanospheres; Step 3: The natamycin-graphene nanoparticles are immersed in 30 mg/L chitosan acetic acid solution for 10 min, and then dried with nitrogen; Step 4: Repeat steps 1 and 2 for 3 times; Step 5: The nanospheres are immersed in 4,4'-diaminostilbene-2,2'-disulfonic acid solution (DAS) for 30 min, dried with nitrogen, and irradiated by UV for 30 min; Step 6: The prepared natamycin self-assembled nanospheres are hydrated to form an antibacterial film.
Claims (8)
- Editorial Note 2020102161 There is only one page of the claimWhat is claimed is: 1. A self-assembled nanosphere and its prparation method and application method, characterized in that: immersing loading material into dispersion of graphene oxide for 10 min under ultrasonic condition, and then drying with nitrogen to form nanoparticles, and then immersing the nanoparticles into chitosan solution for 10 min, then drying with nitrogen, and then rpeated the above steps to prpam multilayer nanospheres; then immersing the multilayer nanospheres into 4,4'-diaminostilbene-2,2'-disulfonic acid solution (DAS) for 30min and then taking the multilayer nanospheres out, drying with nitrogen, and irradiating by UV to prepare sustained-release self-assembled nanospheres.
- 2. The self-assembled nanosphem according to claim 1, characterized in that a solid content of homogeneous dispersion ofthe graphene oxide in water is 0.1-0.5g/L.
- 3. The self-assembled nanosphere according to claim 1, characterized in that a nano pore size of the graphene oxide is adjusted by changing ultrasonic time, so as to control a selective release of the loading material.
- 4. The self-assembled nanosphere according to claim 1, characterized in that a lease rate of the loading materialis pocisely controlled by accurately controlling the number oflayers ofgraphene.
- 5. The self-assembled nanosphere according to claim 1, characterized in that the chitosan with positive charge ionized in water is able to self-assemble with the graphene oxide with negative charge though electrostatic interaction and hydrogen bonding.
- 6. The self-assembled nanosphere according to claim 1, characterized in that a immersion time of photosensitive crosslinker DAS is 15 to 45 min, and a cross-linking reaction takes place 20 to 40 min under ultraviolet lamp irradiation, to form a covalent cross-linked network in multilayer film and enhance a stability of the nanosphere.
- 7. The self-assembled nanosphere according to claim 1, characterized in that the self-assembled nanosphere are prpared into spheres for controlling the sustained-oelease of small molecules; further, an outer layer is chitosan, and the chitosan is configured to be hydrated into a film when used.
- 8. The self-assembled nanosphere according to claim 1, characterized in that the loading material is added into the graphene oxide by ionic gelation method or bottom-up anti-solvent prcipitation method.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114197088A (en) * | 2021-11-09 | 2022-03-18 | 华南理工大学 | Method for preparing nano-fiber or nano-microsphere by ultrasonic induction and film formed by nano-material |
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2020
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114197088A (en) * | 2021-11-09 | 2022-03-18 | 华南理工大学 | Method for preparing nano-fiber or nano-microsphere by ultrasonic induction and film formed by nano-material |
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