AU2019100706A4 - Oligomeric proanthocyanidin extract mixed STOCK solution capable of scavenging free radicals from air and preparation and use methods thereof - Google Patents
Oligomeric proanthocyanidin extract mixed STOCK solution capable of scavenging free radicals from air and preparation and use methods thereof Download PDFInfo
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- AU2019100706A4 AU2019100706A4 AU2019100706A AU2019100706A AU2019100706A4 AU 2019100706 A4 AU2019100706 A4 AU 2019100706A4 AU 2019100706 A AU2019100706 A AU 2019100706A AU 2019100706 A AU2019100706 A AU 2019100706A AU 2019100706 A4 AU2019100706 A4 AU 2019100706A4
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/01—Deodorant compositions
- A61L9/013—Deodorant compositions containing animal or plant extracts, or vegetable material
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L9/00—Disinfection, sterilisation or deodorisation of air
- A61L9/015—Disinfection, sterilisation or deodorisation of air using gaseous or vaporous substances, e.g. ozone
- A61L9/04—Disinfection, sterilisation or deodorisation of air using gaseous or vaporous substances, e.g. ozone using substances evaporated in the air without heating
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- B05B17/04—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods
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Abstract
The present invention relates to an oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air and preparation and use methods thereof. An oligomeric proanthocyanidin A is mixed with an aqueous solvent dissolved with an oligomeric proanthocyanidin B, and more oligomeric proanthocyanidins are incorporated in the same case by using the aqueous solvent containing oligomeric proanthocyanidins as a guide solvent, so that the obtained oligomeric proanthocyanidin extract mixed stock solution has high purity; the final product is a liquid phase, which is more favorable for volatilization and application to a larger space. The oligomeric proanthocyanidin extract mixed stock solution has a 50 inhibiting concentration (IC50) of 11.6 g/ml for superoxide anion radicals, an IC50 of 9.8 pg/ml for hydroxyl radicals, and an IC50 of 1.8 g/ml for DPPH, and has excellent performance of scavenging free radicals, and in general, more than 94% of free radicals in the space can be scavenged within 1 hour, so that the health of indoor personnel is guaranteed while the environment is optimized, the work efficiency is improved or the quality of life is improved.
Description
OLIGOMERIC PROANTHOCYANIDIN EXTRACT MIXED STOCK SOLUTION
CAPABLE OF SCAVENGING FREE RADICALS FROM AIR AND PREPARATION
AND USE METHODS THEREOF
FIELD OF THE INVENTION
The present invention relates to the technical field of disinfection, sterilization or deodorization of air, in particular to an oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air and preparation and use methods thereof.
BACKGROUND OF THE INVENTION
With the rapid development of human civilization, science and technologies have created enormous productivity for human beings, and also brought a large number of by-products, including increasing free radicals. The extensive use of chemical agents, the increase of automobile exhaust and industrial waste gas and the like result in the production of the free radicals.
Studies have shown that the formation of free radicals is complex and diverse. For example, free radicals may be formed by heating, burning, lighting, contact of one substance with another substance or any kind of chemical reaction, that is, human civilization activities in daily life constantly manufacture more free radicals to destroy the ecological environment. These free radicals have long exceeded the standards that human and life can maintain a normal balance, so human health is facing unprecedented severe challenges.
Proanthocyanidins are a mixture of bioflavonoids with special molecular structures. Oligomeric proanthocyanidins (OPC) of the proanthocyanidins are an internationally accepted natural antioxidant that is effective in scavenging free radicals from humans, and are generally grape seed extract or French coastal pine bark extract with strong in-vivo activity. Experiments have proved that the free radical oxidation resisting capacity of OPC is 50 times that of vitamin E and 20 times that of vitamin C.
Based on the excellent oxidation resistance, the proanthocyanidins have been widely applied in food and cosmetics, but have not been considered to scavenge free radicals from air.
SUMMARY OF THE INVENTION
In order to solve the problem that the excellent oxidation resistance of proanthocyanidins is not applied to scavenge free radicals from air in the prior art, the invention provides an
2019100706 27 Jun 2019 optimized oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air and preparation and use methods thereof.
According to the technical solution adopted by the present invention, an oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air consists of the following components in parts by weight:
1-5 parts of oligomeric proanthocyanidin A; and
90-100 parts of aqueous solvent;
where 480-502 mg/lOOmL of oligomeric proanthocyanidin B is dissolved in the aqueous solvent.
Preferably, the oligomeric proanthocyanidin A and the oligomeric proanthocyanidin B have a degree of polymerization of 2 to 5.
A preparation method of the oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air comprises the following steps:
step 1: preparing an oligomeric proanthocyanidin A;
step 2: preparing an aqueous solvent;
step 3: mixing 1-5 parts of oligomeric proanthocyanidin A and 90-100 parts of aqueous solvent; and step 4: sterilizing to obtain an oligomeric proanthocyanidin extract mixed stock solution.
Further, step 1 specifically includes the following steps:
Step 1.1: selecting grape seeds in batches, washing and drying the grape seeds until a moisture content of the grape seeds is 6% or less, and pulverizing the grape seeds;
In the present invention, the grape seeds are mainly grape seeds of Western Australia Syrah grape, the proanthocyanidin content of which is about 40% higher than that of Carignan grape seeds, facilitating the extraction of proanthocyanidins.
Step 1.2: standing the pulverized grape seeds in an oxygen-free box of 40°C for 60-72 hours;
Generally in the present invention, in order to ensure the stability of product properties, the oxygen-free box is filled with nitrogen to form a nitrogen oxygen-free environment.
Step 1.3: taking out the grape seed powder, extracting the grape seed powder after standing with 80% (in volume fraction) didealdehyde ethanol as a pressurized solvent for T time, and repeating the extraction by N times;
Step 1.4: combining all the extracting solutions, and standing at a temperature of 60°C for 24 hours;
Step 1.5: filtering impurities from all the extracting solutions to obtain clear crude proanthocyanidin extract filtrate, and cooling to the filtrate to a temperature of 35°C;
2019100706 27 Jun 2019
Step 1.6: purifying the clear crude proanthocyanidin extract filtrate by flowing through an AB-8 resin adsorption column at a preset rate to obtain a proanthocyanidin extract;
Generally in the present invention, the flow rate of the filtrate flowing through the AB-8 resin adsorption column is 1.0 ml/min.
Step 1.7: washing the purified proanthocyanidin with water to remove impurities to obtain an oligomeric proanthocyanidin A attached to the resin adsorption column.
Preferably, in step 1.3, T is 8-12 minutes, the extraction temperature is 65°C, the extraction pressure is 19.2 MPa, and N is 2-4.
Generally in the present invention, T is 9 minutes and the extraction is repeated by 3 times.
Further, step 2 specifically includes the following steps:
Step 2.1: taking grape seeds in batches, washing and drying the grape seeds until a moisture content of the grape seeds is 6% or less, and pulverizing the grape seeds;
Step 2.2: performing solid fermentation on the pulverized grape seeds for 61-72 hours;
In the present invention, aspergillus niger as strains is generally inoculated to the grape seeds, and fermented strains are obtained by activation, primary expanding culture and secondary expanding culture. The strains are inoculated to the pre-treated grape seeds according to a certain ratio, solid fermentation is performed at a temperature of 25°C and a humidity of 80-90% for 65 hours after mixing, and more proanthocyanidins encapsulated in the grape seed coats are released by means of enzymes produced in the growth process of aspergillus niger.
Step 2.3: completely immersing the treated grape seeds into 80% (in volume fraction) didealdehyde ethanol in a volume ratio of 1 :(15-20);
Step 2.4: irradiating the solution of step 2.3 with an ultraviolet light source at a frequency of 2 to 6 times per hour and not less than 5 minutes in a single time for 2 hours;
In the present invention, the use of ultraviolet light helps to increase the leaching rate of proanthocyanidins in the grape seeds while maintaining the purity of the didealdehyde ethanol.
Step 2.5: filtering the precipitates out from the solution treated in step 2.4, boosting to 200-220 MPa, then standing for 20 minutes, and filtering the precipitated out again;
In the present invention, the precipitate is filtered out twice to ensure that the finally obtained aqueous solvent is clear.
Step 2.6: obtaining an aqueous solvent dissolved with an oligomeric proanthocyanidin B.
Preferably, in step 1.1 and step 2.1, the grape seeds are pulverized to 200 meshes.
2019100706 27 Jun 2019
Preferably, in step 1.3, the aqueous solvent dissolved with the oligomeric proanthocyanidin B is used to wash the resin adsorption column and mix with the oligomeric proanthocyanidin A to obtain an oligomeric proanthocyanidin extract mixed stock solution.
In the present invention, the oligomeric proanthocyanidin A is further dissolved by the aqueous solvent containing the oligomeric proanthocyanidin B to increase the concentration of oligomeric proanthocyanidins in the final product, thereby ensuring the effect of scavenging free radicals.
According to a use method of the oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air, based on the volume A of the current space, A/(50-500) ml of the oligomeric proanthocyanidin extract mixed stock solution is taken each time, and is continuously dispersed into particles having a diameter of not more than 8 qm by an ultrasonic vibrator driven by an ultrasonic generator.
In the present invention, single amount of the oligomeric proanthocyanidin extract mixed stock solution is determined according to the size of the space to be treated. Generally, for a space of 20 cubic meters, more than 0.4 ml of the solution is dispersed and volatilized single time.
In the present invention, because the whole product is an aqueous reagent dissolved with oligomeric proanthocyanidins, the product can be directly fetched and ultrasonically dispersed with the ultrasonic vibrator through reasonable setting in the whole operation process, but does not need to be mixed into water.
Preferably, in the particles, the o-phenolic hydroxyl groups of B rings in the proanthocyanidin molecules capture free radicals generated in the oxidation process, and the formed free radicals are stabilized by forming intra-molecular hydrogen bonds, semiquinone free radicals or o-benzoquinone to interrupt the reaction of free radical chains.
The present invention provides an optimized oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air and preparation and use methods thereof, where the oligomeric proanthocyanidin A is mixed with the aqueous solvent dissolved with the oligomeric proanthocyanidin B, and more oligomeric proanthocyanidins are incorporated in the same case by using the aqueous solvent containing oligomeric proanthocyanidins as a guide solvent, so that the obtained oligomeric proanthocyanidin extract mixed stock solution has high purity; the final product is a liquid phase, which is more favorable for volatilization and application to a larger space.
The oligomeric proanthocyanidin extract mixed stock solution of the present invention has a 50% inhibiting concentration (IC50) of 11.6 qg/ml for superoxide anion radicals, an
IC50 of 9.8 qg/ml for hydroxyl radicals, and an IC50 of 1.8 qg/ml for DPPH, and has
2019100706 27 Jun 2019 excellent performance of scavenging free radicals, in general, more than 94% of free radicals in the space can be scavenged within 1 hour, so that the health of indoor personnel is guaranteed while the environment is optimized, the work efficiency is improved or the quality of life is improved.
DETAILED DESCRIPTION OF EMBODIMENTS
The following further describes the present invention in detail in combination with embodiments, but the scope of the present invention is not limited thereto.
Embodiment 1
Disclosed is an oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air. 1 part of oligomeric proanthocyanidin A and 90 parts of aqueous solvent are mixed and sterilized to obtain the oligomeric proanthocyanidin extract mixed stock solution, where 480 mg/lOOmL of oligomeric proanthocyanidin B is dissolved into the aqueous solvent.
The preparation of the oligomeric proanthocyanidin A includes the following steps: taking grape seeds of Western Australia Syrah grape in batches, washing and drying the grape seeds until a moisture content of the grape seeds is 6% or less, and pulverizing the grape seeds; standing the pulverized grape seeds in an oxygen-free box of 40°C for 60 hours; taking out the grape seed powder, extracting the grape seed powder after standing with 80% (in volume fraction) didealdehyde ethanol as a pressurized solvent for 9 minutes, and repeating the extraction by 3 times; combining all the extracting solutions, and standing at 60°C for 24 hours; filtering impurities from all the extracting solutions to obtain clear crude proanthocyanidin extract filtrate, and cooling the filtrate to 35°C; purifying the clear crude proanthocyanidin extract filtrate by flowing through an AB-8 resin adsorption column at a rate of 1.0 ml/min to obtain proanthocyanidin extract; and washing the purified proanthocyanidin with water to remove impurities to obtain an oligomeric proanthocyanidin A attached to the resin adsorption column.
The preparation of the aqueous solvent includes the following steps: taking grape seeds of Western Australia Syrah grape in batches, washing and drying the grape seeds until a moisture content of the grape seeds is 6% or less, and pulverizing the grape seeds; performing solid fermentation on the pulverized grape seeds for 60 hours; completely immersing the treated grape seeds into 80% (in volume fraction) didealdehyde ethanol in a volume ratio of 1: 15; irradiating the solution with an ultraviolet light source at a frequency of 2 times per hour and not less than 5 minutes in a single time for 2 hours; filtering the precipitates out from the
2019100706 27 Jun 2019 solution, boosting to 200 MPa, then standing for 20 minutes, and filtering the precipitated out again; and obtaining an aqueous solvent dissolved with an oligomeric proanthocyanidin B.
The aqueous solvent dissolved with the oligomeric proanthocyanidin B is used to wash the resin adsorption column and mix with the oligomeric proanthocyanidin A to obtain an oligomeric proanthocyanidin extract mixed stock solution.
Embodiment 2
Disclosed is an oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air. 5 parts of oligomeric proanthocyanidin A and 100 parts of aqueous solvent are mixed and sterilized to obtain the oligomeric proanthocyanidin extract mixed stock solution, where 502 mg/lOOmL of oligomeric proanthocyanidin B is dissolved into the aqueous solvent.
The preparation of the oligomeric proanthocyanidin A includes the following steps: taking grape seeds of Western Australia Syrah grape in batches, washing and drying the grape seeds until a moisture content of the grape seeds is 6% or less, and pulverizing the grape seeds; standing the pulverized grape seeds in an oxygen-free box of 40°C for 72 hours; taking out the grape seed powder, extracting the grape seed powder after standing with 80% (in volume fraction) didealdehyde ethanol as a pressurized solvent for 9 minutes, and repeating the extraction by 3 times; combining all the extracting solutions, and standing at 60°C for 24 hours; filtering impurities from all the extracting solutions to obtain clear crude proanthocyanidin extract filtrate, and cooling the filtrate to 35°C; purifying the clear crude proanthocyanidin extract filtrate by following through an AB-8 resin adsorption column at a rate of 1.0 ml/min to obtain proanthocyanidin extract; and washing the purified proanthocyanidin with water to remove impurities to obtain an oligomeric proanthocyanidin A attached to the resin adsorption column.
The preparation of the aqueous solvent includes the following steps: taking grape seeds of Western Australia Syrah grape in batches, washing and drying the grape seeds until a moisture content of the grape seeds is 6% or less, and pulverizing the grape seeds; performing solid fermentation on the pulverized grape seeds for 72 hours; completely immersing the treated grape seeds into 80% (in volume fraction) didealdehyde ethanol in a volume ratio of 1: 20; irradiating the solution with an ultraviolet light source at a frequency of 6 times per hour and not less than 5 minutes in a single time for 2 hours; filtering the precipitates out from the solution, boosting to 220 MPa, then standing for 20 minutes, and filtering the precipitated out again; and obtaining an aqueous solvent dissolved with an oligomeric proanthocyanidin B.
2019100706 27 Jun 2019
The aqueous solvent dissolved with the oligomeric proanthocyanidin B is used to wash the resin adsorption column and mix with the oligomeric proanthocyanidin A to obtain an oligomeric proanthocyanidin extract mixed stock solution.
Embodiment 3
Disclosed is an oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air. 1.23 parts of oligomeric proanthocyanidin A and 98.77 parts of aqueous solvent are mixed and sterilized to obtain the oligomeric proanthocyanidin extract mixed stock solution, where 499.5 mg/lOOmL of oligomeric proanthocyanidin B is dissolved into the aqueous solvent.
The preparation of the oligomeric proanthocyanidin A includes the following steps: taking grape seeds of Western Australia Syrah grape in batches, washing and drying the grape seeds until a moisture content of the grape seeds is 6% or less, and pulverizing the grape seeds; standing the pulverized grape seeds in an oxygen-free box of 40°C for 70 hours; taking out the grape seed powder, extracting the grape seed powder after standing with 80% (in volume fraction) didealdehyde ethanol as a pressurized solvent for 9 minutes, and repeating the extraction by 3 times; combining all the extracting solutions, and standing at 60°C for 24 hours; filtering impurities from all the extracting solutions to obtain clear crude proanthocyanidin extract filtrate, and cooling to 35°C; purifying the clear crude proanthocyanidin extract filtrate by flowing through an AB-8 resin adsorption column at a rate of 1.0 ml/min to obtain proanthocyanidin extract; and washing the purified proanthocyanidin with water to remove impurities to obtain an oligomeric proanthocyanidin A attached to the resin adsorption column.
The preparation of the aqueous solvent includes the following steps: taking grape seeds of Western Australia Syrah grape in batches, washing and drying the grape seeds until a moisture content of the grape seeds is 6% or less, and pulverizing the grape seeds; performing solid fermentation on the pulverized grape seeds for 65 hours; completely immersing the treated grape seeds into 80% (in volume fraction) didealdehyde ethanol in a volume ratio of 1:17.5; irradiating the solution with an ultraviolet light source at a frequency of 5 times per hour and not less than 5 minutes in a single time for 2 hours; filtering the precipitates out from the solution, boosting to 215 MPa, then standing for 20 minutes, and filtering the precipitated out again; and obtaining an aqueous solven * t dissolved with an oligomeric proanthocyanidin B.
2019100706 27 Jun 2019
The aqueous solvent dissolved with the oligomeric proanthocyanidin B is used to wash the resin adsorption column and mix with the oligomeric proanthocyanidin A to obtain an oligomeric proanthocyanidin extract mixed stock solution.
The same batch of grape seeds of Western Australia Syrah grape is taken and divided into 4 parts;
80% didealdehyde ethanol is taken and divided into 4 parts;
Three kinds of oligomeric proanthocyanidin extract mixed stock solutions, i.e., group A, group B and group C, are prepared according to Embodiments 1-3, respectively;
An oligomeric proanthocyanidin A is prepared with steps 1.1 to 1.7, and is directly dissolved into 80% didealdehyde ethanol to obtain a group D as a contrast group;
Experimental content:
The content of proanthocyanidins in each group of products is separately tested;
ml of each product is taken, dispersed with the same ultrasonic vibrator and applied to a closed space of 20 cubic meters, and 50 inhibiting concentrations (IC50) for superoxide anion radicals, hydroxyl radicals and DPPH are tested;
The results are shown in Table 1.
Group | Content of proanthocyanidins | IC50 for superoxide anion radicals | IC50 for hydroxyl radicals | IC50 for DPPH |
Group A | 580 mg/lOOmL | 15.4 mg/m3 | 10.4 mg/m3 | 1.8 mg/m3 |
Group B | 1020 mg/lOOmL | 13.1 mg/m3 | 8.4 mg/m3 | 1.2 mg/m3 |
Group C | 615 mg/lOOmL | 15.2 mg/m3 | 10.1 mg/m3 | 1.7 mg/m3 |
Group D | 201 mg/lOOmL | 22.7 mg/m3 | 15.9 mg/m3 | 3.7 mg/m3 |
2019100706 27 Jun 2019
Claims (10)
1. An oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air, consisting of the following components in parts by weight:
1-5 parts of oligomeric proanthocyanidin A; and
90-100 parts of aqueous solvent;
wherein 480-502 mg/lOOml of oligomeric proanthocyanidin B is dissolved in the aqueous solvent.
2. The oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air according to claim 1, wherein the oligomeric proanthocyanidin A and the oligomeric proanthocyanidin B have a degree of polymerization of 2 to 5.
3. A preparation method of the oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air according to claim 1 or 2, comprising the following steps:
step 1: preparing an oligomeric proanthocyanidin A;
step 2: preparing an aqueous solvent;
step 3: mixing 1-5 parts of oligomeric proanthocyanidin A and 90-100 parts of aqueous solvent; and step 4: sterilizing to obtain an oligomeric proanthocyanidin extract mixed stock solution.
4. The preparation method of the oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air according to claim 3, wherein step 1 comprises the following steps:
step 1.1: taking grape seeds in batches, washing and drying the grape seeds until a moisture content is 6% or less, and pulverizing the grape seeds;
step 1.2: standing the pulverized grape seeds in an oxygen-free box of 40°C for 60-72 hours;
step 1.3: taking out the grape seed powder, extracting the grape seed powder after standing with 80% (in volume fraction) didealdehyde ethanol as a pressurized solvent
2019100706 27 Jun 2019 for T time, and repeating the extraction by N times;
step 1.4: combining all the extracting solutions, and standing at 60 °C for 24 hours; step 1.5: filtering impurities from all the extracting solutions to obtain a clear crude proanthocyanidin extract filtrate, and cooling the filtrate to 35 °C;
step 1.6: purifying the clear crude proanthocyanidin extract filtrate by flowing through an AB-8 resin adsorption column at a preset rate to obtain a proanthocyanidin extract; and step 1.7: washing the purified proanthocyanidin with water to remove impurities to obtain an oligomeric proanthocyanidin A attached to the resin adsorption column.
5. The preparation method of the oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air according to claim 4, wherein in the step 1.3, T is 8-12 minutes, the extraction temperature is 65°C, the extraction pressure is 19.2 MPa, and N is 2-4.
6. The preparation method of the oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air according to claim 3, wherein the step 2 comprises the following steps:
step 2.1: taking grape seeds in batches, washing and drying the grape seeds until a moisture content of the grape seeds is 6% or less, and pulverizing the grape seeds; step 2.2: performing solid fermentation on the pulverized grape seeds for 61-72 hours; step 2.3: completely immersing the treated grape seeds into 80% didealdehyde ethanol in a volume ratio of 1:( 15-20);
step 2.4: irradiating the solution of step 2.3 with an ultraviolet light source at a frequency of 2 to 6 times per hour and not less than 5 minutes in a single time for 2 hours;
step 2.5: filtering the precipitates out from the solution treated in step 2.4, boosting to 200-220 MPa, then standing for 20 minutes, and filtering the precipitated out again; and step 2.6: obtaining an aqueous solvent dissolved with an oligomeric proanthocyanidin B.
7. The preparation method of the oligomeric proanthocyanidin extract mixed stock io
2019100706 27 Jun 2019 solution capable of scavenging free radicals from air according to claim 4 or 6, wherein in the step 1.1 and the step 2.1, the grape seeds are pulverized to 200 meshes.
8. The preparation method of the oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air according to claim 4, wherein in the step 1.3, the aqueous solvent dissolved with the oligomeric proanthocyanidin B is used to wash the resin adsorption column and mix with the oligomeric proanthocyanidin A to obtain the oligomeric proanthocyanidin extract mixed stock solution.
9. A use method of the oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air according to claim 1 or 2, wherein based on the volume A of the current space, A/(50-500) ml of the oligomeric proanthocyanidin extract mixed stock solution is taken each time, and is continuously dispersed into particles having a diameter of not more than 8 pm by an ultrasonic vibrator driven by an ultrasonic generator.
10. The use method of the oligomeric proanthocyanidin extract mixed stock solution capable of scavenging free radicals from air according to claim 9, wherein in the particles, the o-phenolic hydroxyl groups of B rings in the proanthocyanidin molecules capture free radicals generated in the oxidation process, and the formed free radicals are stabilized by forming intra-molecular hydrogen bonds, semiquinone free radicals or o-benzoquinone to interrupt the reaction of free radical chains.
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