AU2018281936A1

AU2018281936A1 - Compositions for biological systems and methods for preparing and using the same

Info

Publication number: AU2018281936A1
Application number: AU2018281936A
Authority: AU
Inventors: Soumyajit Banerjee Mustafi; Christopher D. Jones
Original assignee: Smart Surgical Inc
Current assignee: Smart Surgical Inc
Priority date: 2017-06-10
Filing date: 2018-05-25
Publication date: 2020-01-30
Also published as: WO2018226435A1

Description

COMPOSITIONS FOR BIOLOGICAL SYSTEMS AND METHODS FOR PREPARING AND USING THE SAME

BACKGROUND OF THE INVENTION [0001] The field of the invention generally relates to cellular allograft for tissue redevelopment, including bone redevelopment.

[0002] The sources of cellular allograft compositions have historically involved adult bone marrow, though recently, placental fluid has been an experimental source.

SUMMARY OF THE INVENTION [0003] In one embodiment of the present disclosure, a composition for influencing biological growth includes live cells, a fluid comprising dextrose, a protectant, and a first cytokine having a first concentration within the composition, selected from the group consisting of BDNF at between 492 picograms per ml and 915 picograms per ml, bNGF at between 14 picograms per ml and 26 picograms per ml, EGF at between 715 picograms per ml and 1329 picograms per ml, Eotaxin at between 37 picograms per ml and 69 picograms per ml, FGF-2 at between 28 picograms per ml and 53 picograms per ml, GM-CSF at between 7 picograms per ml and 15 picograms per ml, Gro-α at between 2974 picograms per ml and 5524 picograms per ml, HGF at between 352 picograms per ml and 656 picograms per ml, IFN-α at between 253 picograms per ml and 472 picograms per ml, IFN-γ at between 33 picograms per ml and 63 picograms per ml, IL-10 at between 67 picograms per ml and 126 picograms per ml, IL-12p70 at between 9 picograms per ml and 19 picograms per ml, IL13 at between 11 picograms per ml and 22 picograms per ml, IL-15 at between 30 picograms per ml and 57 picograms per ml, IL-17A at between 19 picograms per ml and 37 picograms per ml, IL-18 at between 78 picograms per ml and 146 picograms per ml, IL-Ια at between 712 picograms per ml and 1324 picograms per ml, IL-Ιβ at between 14 picograms per ml and 28 picograms per ml, IL-IRA at between 2215 picograms per ml and 4114 picograms per ml, IL-2 at between 16 picograms per ml and 31 picograms per ml, IL-21 at between 370 picograms per ml and 689 picograms per ml, IL-22 at between 16 picograms per ml and 32 picograms per ml, IL-23 at between 201 picograms per ml and 376 picograms per ml, IL-27 at between 21 picograms per ml and 40 picograms per ml, IL-31 at between 11 picograms per

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PCT/US2018/034584 ml and 22 picograms per ml, IL-4 at between 17 picograms per ml and 33 picograms per ml, IL-5 at between 15 picograms per ml and 28 picograms per ml, IL-6 at between 50 picograms per ml and 95 picograms per ml, IL-7 at between 26 picograms per ml and 50 picograms per ml, IL-8 at between 4504 picograms per ml and 8366 picograms per ml, IL-9 at between 131 picograms per ml and 245 picograms per ml, IP-10 at between 469 picograms per ml and 873 picograms per ml, LIF at between 41 picograms per ml and 77 picograms per ml, MCP-1 at between 14,480 picograms per ml and 26,893 picograms per ml, MIP-Ια at between 303 picograms per ml and 565 picograms per ml, MIP-Ιβ at between 375 picograms per ml and 698 picograms per ml, PDGF-BB at between 807 picograms per ml and 1500 picograms per ml, PIGF-1 at between 743 picograms per ml and 1381 picograms per ml, RANTES at between 3277 picograms per ml and 6087 picograms per ml, SCF at between 23 picograms per ml and 45 picograms per ml, SDF-Ια at between 1114 picograms per ml and 2071 picograms per ml, TNF- a at between 42 picograms per ml and 80 picograms per ml, TNF-β at between 12 picograms per ml and 25 picograms per ml, VEGF-A at between 481 picograms per ml and 895 picograms per ml, and VEGF-D at between 46 picograms per ml and 88 picograms per ml.

[0004] In another embodiment of the present disclosure, a composition for influencing biological growth includes live cells, a fluid comprising dextrose , a protectant, VEGF-A, at a concentration within the composition of between 481 picograms per ml and 895 picograms per ml, PDGF-BB, at a concentration within the composition of between 807 picograms per ml and 1500 picograms per ml, EGF, at a concentration within the composition of between 715 picograms per ml and 1329 picograms per ml, SCF, at a concentration within the composition of between 23 picograms per ml and 45 picograms per ml, and IL-IRA, at a concentration within the composition of between 2215 picograms per ml and 4114 picograms per ml.

[0005] In still another embodiment of the present disclosure, a composition for influencing biological growth includes live cells, a fluid comprising dextrose, a protectant, and wherein the composition includes the following cytokines with their concentration within the composition: BDNF at between 492 picograms per ml and 915 picograms per ml, bNGF at between 14 picograms per ml and 26 picograms per ml, EGF at between 715 picograms per ml and 1329 picograms per ml, Eotaxin at between 37 picograms per ml and 69 picograms per ml, FGF-2 at between 28 picograms per ml and 53 picograms per ml, GMCSF at between 7 picograms per ml and 15 picograms per ml, Gro-α at between 2974 picograms per ml and 5524 picograms per ml, HGF at between 352 picograms per ml and

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656 picograms per ml, IFN-α at between 253 picograms per ml and 472 picograms per ml, IFN-γ at between 33 picograms per ml and 63 picograms per ml, IL-10 at between 67 picograms per ml and 126 picograms per ml, IL-12p70 at between 9 picograms per ml and 19 picograms per ml, IL-13 at between 11 picograms per ml and 22 picograms per ml, IL-15 at between 30 picograms per ml and 57 picograms per ml, IL-17A at between 19 picograms per ml and 37 picograms per ml, IL-18 at between 78 picograms per ml and 146 picograms per ml, IL-Ια at between 712 picograms per ml and 1324 picograms per ml, IL-Ιβ at between 14 picograms per ml and 28 picograms per ml, IL-IRA at between 2215 picograms per ml and 4114 picograms per ml, IL-2 at between 16 picograms per ml and 31 picograms per ml, IL-21 at between 370 picograms per ml and 689 picograms per ml, IL-22 at between 16 picograms per ml and 32 picograms per ml, IL-23 at between 201 picograms per ml and 376 picograms per ml, IL-27 at between 21 picograms per ml and 40 picograms per ml, IL-31 at between 11 picograms per ml and 22 picograms per ml, IL-4 at between 17 picograms per ml and 33 picograms per ml, IL-5 at between 15 picograms per ml and 28 picograms per ml, IL-6 at between 50 picograms per ml and 95 picograms per ml, IL-7 at between 26 picograms per ml and 50 picograms per ml, IL-8 at between 4504 picograms per ml and 8366 picograms per ml, IL-9 at between 131 picograms per ml and 245 picograms per ml, IP-10 at between 469 picograms per ml and 873 picograms per ml, LIF at between 41 picograms per ml and 77 picograms per ml, MCP-1 at between 14,480 picograms per ml and 26,893 picograms per ml, MIP-la at between 303 picograms per ml and 565 picograms per ml, ΜΙΡ-Ιβ at between 375 picograms per ml and 698 picograms per ml, PDGF-BB at between 807 picograms per ml and 1500 picograms per ml, PIGF-1 at between 743 picograms per ml and 1381 picograms per ml, RANTES at between 3277 picograms per ml and 6087 picograms per ml, SCF at between 23 picograms per ml and 45 picograms per ml, SDF-Ια at between 1114 picograms per ml and 2071 picograms per ml, TNF- a at between 42 picograms per ml and 80 picograms per ml, TNF-β at between 12 picograms per ml and 25 picograms per ml, VEGFA at between 481 picograms per ml and 895 picograms per ml, and VEGF-D at between 46 picograms per ml and 88 picograms per ml.

BRIEF DESCRIPTION OF THE DRAWINGS [0006] FIG. 1 illustrates a process for forming a composition for implantation within a patient according to a first embodiment.

[0007] FIG. 2 illustrates a container having first and second layers placed therein.

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PCT/US2018/034584 [0008] FIG. 3 illustrates a general mechanism for applying a centrifugal force on a container.

[0009] FIG. 4 illustrates the container of FIG. 2 following the application of a centrifugal force.

[0010] FIG. 5 illustrates the container of FIG. 4 after the removal of certain material.

[0011] FIG. 6 illustrates a process for forming a composition for implantation within a patient according to a second embodiment.

[0012] FIG. 7 illustrates a container having first and second layers placed therein.

[0013] FIG. 8 illustrates a general mechanism for applying a centrifugal force on a container.

[0014] FIG. 9 illustrates the container of FIG. 7 following the application of a centrifugal force.

[0015] FIG. 10 illustrates the container of FIG. 9 after the removal of certain material.

[0016] FIG. 11 illustrates a process for forming a composition for implantation within a patient according to a third embodiment.

[0017] FIG. 12 illustrates first and second flexible containers during an operation.

[0018] FIG. 13 illustrates a general mechanism for applying a centrifugal force on the second flexible container of FIG. 12.

[0019] FIG. 14 illustrates first and second flexible containers of FIG. 12 during another operation.

[0020] FIG. 15 illustrates the second flexible container following the application of a centrifugal force.

[0021] FIG. 16 illustrates a process for forming a composition for implantation within a patient according to a fourth embodiment.

[0022] FIG. 17 illustrates a container having first and second layers placed therein.

[0023] FIG. 18 illustrates a general mechanism for applying a centrifugal force on a container.

[0024] FIG. 19 illustrates the container of FIG. 17 following the application of a centrifugal force.

[0025] FIG. 20 illustrates the container of FIG. 19 after the removal of certain material.

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PCT/US2018/034584 [0026] FIG. 21 illustrates a process for forming a composition for implantation within a patient according to a fifth embodiment.

[0027] FIG. 22 illustrates a container having first and second layers placed therein.

[0028] FIG. 23 illustrates a general mechanism for applying a centrifugal force on a container.

[0029] FIG. 24 illustrates the container of FIG. 22 following the application of a centrifugal force.

[0030] FIG. 25 illustrates the container of FIG. 24 after the removal of certain material.

[0031] FIGS. 26A-26C illustrate two vertebrae during a spinal fusion procedure in a patient, according to an embodiment.

[0032] FIGS. 27A-27B illustrate two bone portions during a fusion procedure in a patient, according to an embodiment.

[0033] FIGS. 28A-28B illustrate soft tissue during a procedure in a patient, according to an embodiment.

DETAILED DESCRIPTION OF THE ILLUSTRATED EMBODIMENTS [0034] The present invention relates to a composition of cellular allograft that is derived at least partially from umbilical cord blood (UCB), as well as its manufacture and use. Compositions for accelerating or optimizing biological processes or initiating new biological processes may be produced in accordance with the teachings described herein. Cells and growth factors may advantageously be obtained or derived from umbilical cord blood, and may inherently benefit from the advantage that umbilical cord blood includes native cell populations that can support a universal donor application. An inherently increased cellular health may also sustain the benefit of the cellular allograft. A number of cytokines are produced and/or maintained at controlled concentrations or profiles within embodiments of the compositions, in order to provide specific individual and/or collaborative therapeutic functions, at particular levels or rates.

[0035] In some embodiments of the compositions, albumin is added. It should be noted that the use of “in some embodiments” herein is intended to mean “in any of the embodiments or combinations of any of the embodiments in which the described element has the possibility or capability of being added.” In studies performed by the Applicants, albumin has been shown to increase the viability of cellular components of blood during freezing and thawing procedures that are common during the processing of such compositions. It is

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PCT/US2018/034584 thought that albumin may enhance cellular viability post-thaw by acting as a protein stabilizer, preventing the aggregation of proteins in solution as well as decreasing the amount of incorrect protein folding. Correct folding of proteins allows the proteins to, for example, achieve their desired three-dimensional structure. However, misfolded proteins have modified or toxic functionality. Albumin has also been shown to affect the adherence of cells to flasks used during the culturing process (culture flasks). Albumin additionally can enhance the rate of differentiation of umbilical cord blood cells, for example stem cells. Albumin can serve as an excellent cryopreservative, at least in part because of its antiapoptotic properties. Albumin can prevent cell death by activation of the phosphatidylinositol 3-kinase (PI3K)/AKT pathway which elicits anti-apoptotic pro-survival pathways in different cellular systems. Aberrant ROS generation facilitates mitochondrial depolarization leading to autophagy and apoptosis. Albumin prevents depolarization of the mitochondria Albumin confers an anti-apoptotic effect through regulation of the p38 MAPK, and Akt/GSK-3p/Mcll/caspase-3 signaling pathways. Sustaining mTORCl by albumin also prevents autophagic cell death. Though albumin is discussed, other protectants or stabilizers may instead be used, such as dimethyl sulfoxide (DMSO) or CryoGold™. In some embodiments, a combination of protectants and stabilizers may be used.

[0036] In certain compositions a low, acidic pH, such as a pH between about 4.0 and about 5.0, or about 4.4, may be desirable, such as compositions intended for wound care that includes a cell-free extract. A low pH composition may have a microenvironment that promotes the death and rupture of cells, for example, during freezing or thawing. This death and rupture of cells, or the increase thereof, may result in an increase of soluble extracellular protein factors or bioactive agents, including, but not limited to cytokines or growth factors. The word “cytokine,” when used alone herein, can also encompass growth factors. These bioactive agents are thought to be a potentially helpful component in the kinetics of wound healing. An extracellular acidic environment can stimulate Total Nucleated Cells (TNC) in umbilical cord blood, for example, to upregulate the stress related to signaling pathways essential for wound healing. Cells exposed and preserved in an acidic microenvironment can undergo a differential transcriptional program of “regeneration” relevant genes that aid in tissue remodeling. Extracellular signal regulated kinase (ERK1/2), Mitogen activated protein kinase (MAPK), AKT/PKB are some of the stress induced signaling pathways that can be triggered by the acidic environment. Total Nucleated Cells in a media having the low pH, acidic environment will be in a stimulated state in response to the acidosis stress and will be capable of initiating a wound healing mechanism within a significantly shorter time frame.

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PCT/US2018/034584 [0037] An extracellular acidic environment can also stabilize cytokines and growth factors present in the umbilical cord blood. Low-pH-induced changes can be associated with several factors, including, but not limited to, pK(a)s, solubility limitations, eutectic crystallization, and cryoconcentration. The optimal pH of activity (the pH of maximal activity) is correlated with the optimal pH of stability (the pH of maximal stability), allowing the corresponding macromolecules to subsequently tolerate small pH fluctuations that are inevitable with cellular function. Additionally, growth factors and cytokines, such as those disclosed herein, and including Vascular endothelial growth factor (VEGF), can bind to endothelial cells, and the extracellular matrix (ECM) increases at an acidic pH. Thus, the acidic pH in the cryopreservation will maintain the tertiary structures of these particular proteins in a conformation (e.g., 3-dimensional structure) that can easily bind to the cell surface rectors and initiate the signaling cascade.

[0038] Conventional wisdom is that cells and proteins are best cryopreserved if done so at or very near physiological pH (neutral or near neutral), and that shifting the pH balance to either side of the neutral pH (e.g., 7) will significantly reduce the chances of cell survival and degrade protein activity. But these supposed degradative effects, in the low pH area, have not been contextualized in the realm of a low grade acute and chronic stress that triggers signaling pathways. The inventors have learned that, counterintuitively, cryopreserving at an acidic pH will “pulse” the Total Nucleated Cells to activate stress regulated signaling pathways that are strongly coupled to the physiological process of wound healing and tissue regeneration. Thus, Total Nucleated Cells being exposed to acidosis shock/stress (i.e., preconditioned in a low extracellular pH) are better adapted to initiate and operate in the healing process. Similarly, non-cellular components, particularly cytokines and growth factors which have an isoelectric point (pl) that is in the acidic range are more stable and have a tertiary confirmation that can easily bind to the receptors with a lower rate of dissociation (Kd). The low pH cryopreservatives are able to perform the two-sided function of stimulating the Total Nucleated Cells and preserving low molecular weight proteins and growth factors, so that they can perform better during their use (e.g., allogenic use).

[0039] The kinetics or temporal aspects of wound healing may vary depending on the particular makeup of a composition that is added to tissue of a patient. For example, a composition containing cells that include up to about 70% viable cells may include a larger comparative volume or amount of soluble extracellular factors/bioactive agents than a composition containing cells that include greater than about 95% viable cells. The composition with up to about 70% viable cells may thus cause a more rapid wound healing

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PCT/US2018/034584 response than the composition with greater than about 95% viable cells. The use of a relatively low-pH composition as described may thus be utilized in a patient with one or more chronic wounds.

[0040] On the other hand, in other compositions, a more alkaline pH, such as a pH between about 7.0 and about 8.0, or about 7.5, may be desirable, such as compositions intended to mediate a biological healing process using viable cells, such as stem cells. Albumin is capable of helping to control the effect of pH on cells, and can thus be incorporated into compositions such as these for this purpose.

[0041] The Applicants have also observed that umbilical cord blood cells, when cultured in the presence of albumin, differentiate into several different cell types having varied morphologies. By identifying these particular species of cells, post-differentiation, each particular group of cells may be enhanced to create specialty compositions in each case. For example, one or more particular compositions specially formulated for bone healing or bone growth, one or more particular compositions specially formulated for nerve healing or nerve growth, or other compositions for healing or growth of other types of tissue. After the identification of a cell type, the differentiation of stem cells can be directed toward that particular cell type. With each cell type, certain bioactive agents (cytokines, growth factors, etc.) associated with that cell type may be isolated. These bioactive agents may then be used in particular compositions for specialized purposes. As an example, oncostatin M is a cytokine that sticks to, or binds to extracellular matrix (ECM), and has been identified as a growth factor that affects both bones and nerves. For at least this reason, oncostatin M can be incorporated into bone and/or nerve allograft compositions. As another example, bone morphogenetic proteins (BMP) are growth factors or cytokines that can be incorporated into allograft compositions for use with bone.

[0042] A first embodiment of a composition comprises a solution having a first volume and comprising a low pH fluid base comprising dextrose. The word “base,” as used in this particular phrase, is intended to mean a foundational, basal, or holding solution, whether possessing solvent-like properties or not. In some embodiments, a composition may comprise a solution having a volume and comprising a low pH fluid base comprising dextrose and dextran, and also comprising albumin, In some embodiments, the low pH fluid base may comprise LMD (Dextran 40) or an equivalent compound. In some embodiments, the low pH fluid base consists of LMD (Dextran 40). The low pH fluid base of dextrose and dextran, and especially the dextrose component, can act as a proteinaceous protectant by preventing the aggregation of proteins, such as, for example, cytokines. As described, in

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PCT/US2018/034584 some embodiments, albumin may be utilized within the solution. In some embodiments utilizing albumin, the albumin in the solution has a concentration of between about 10 mg/ml (milligram per milliliter) and about 150 mg/ml, or between about 10 mg/ml (milligram per milliliter) and about 120 mg/ml. In some embodiments, the albumin in the solution has a concentration of between about 15 mg/ml (milligram per milliliter) and about 30 mg/ml. In some embodiments, the albumin in the solution has a concentration of between about 18 mg/ml (milligram per milliliter) and about 25 mg/ml. In some embodiments, the albumin in the solution has a concentration of about 20 mg/ml. In some embodiments, the albumin in the solution has a concentration of between about 80 mg/ml and about 150 mg/ml, or between about 50 mg/ml and about 150 mg/ml. In some embodiments, the albumin in the solution has a concentration of between about 80 mg/ml and about 120 mg/ml, or between about 50 mg/ml and about 120 mg/ml, or between about 90 mg/ml and about 110 mg/ml. In some embodiments, the albumin in the solution has a concentration of between about 105 mg/ml and about 150 mg/ml, or between about 105 mg/ml and about 120 mg/ml. Together, the low pH fluid base of dextrose and dextran and the albumin act together as a storage agent or protectant (preservative, stabilizer) by maintaining the integrity of proteins obtained from their source (e.g., umbilical cord blood). Another beneficial use of the low pH fluid base of dextrose (and dextran, where used) is that it provides a relatively low pH. The low pH microenvironment provides a molecular milieu that promotes wound healing by recruiting cells that activate signaling pathways associated with wound healing and tissue regeneration. In embodiments in which albumin is added in a powdered form to the low pH fluid base of dextrose (and dextran), a cost saving is provided, in comparison to, for example, combining the low pH fluid base of dextrose and dextran and albumin in serum.

[0043] A substance such as sodium bicarbonate powder may be added to the solution as needed to control the final pH of the composition. Alternatively, a calcium bicarbonate liquid may be used. For example, in some embodiments, the pH of the composition may be between 4.1 and 7.2. In some embodiments, the pH of the composition may be between 4.1 and 7.4, or in some cases even higher than 7.4. In some embodiments, the pH of the composition may be between 4.1 and 6.2. In some embodiments, the pH of the composition may be between 5.0 and 7.4. In some embodiments, the pH of the composition may be between 5.0 and 6.2. A pH within one of the ranges listed may be achieved in some cases without having to add an additional base - in this case, the word “base” is intended to mean a substance that releases or produces hydroxide ions (OH^-) in aqueous solutions - such as sodium bicarbonate or calcium bicarbonate, based on the inherent characteristics of the

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PCT/US2018/034584 low pH fluid base of dextrose and dextran. In other cases, the additional base may be used in order to achieve a pH within one of the range listed. In certain cases, it may be necessary to use an additional base in order to achieve a pH that is within one of the ranges listed.

[0044] In some embodiments, the albumin may be obtained or derived from commercially available human serum. In other embodiments, the albumin may be obtained or derived from donor-derived serum. In some embodiments, the albumin may be obtained or derived from human umbilical cord blood.

[0045] In some embodiments, the composition may include progenitor cells. In some embodiments, the composition may comprise stem cells. In some embodiments, the stem cells consist of non-expanded cell populations, for example, not expanded by growth in a Petri dish or growth medium/matrix. The overall makeup of umbilical cord blood cell populations and other factors may provide a broader benefit to a patient. The stem cells may comprise one or more types of stem cells, including, but not limited to, hematopoietic stem cells (HSCs) or mesenchymal stem cells (MSCs). In some embodiments, the stem cells may include viable cells and non-viable cells. In some embodiments, the percentage of viable cells among the stem cells may be greater than 70%. In some embodiments, the percentage of viable cells among the stem cells may be greater than 85%. In some embodiments, the percentage of viable cells among the stem cells may be greater than 95%.

[0046] In some embodiments, the composition may comprise live mononuclear cells, which may include, but are not limited to, stem cells or bioactive agents. In some embodiments, the composition comprises between about 2.5 million live mononuclear cells per milliliter (ml) and about 10.2 million live mononuclear cells per milliliter (ml). In some embodiments, the composition comprises between about 9.8 million live mononuclear cells per milliliter (ml) and about 10.2 million live mononuclear cells per milliliter (ml). In some embodiments, the mononuclear cells may be derived from umbilical cord blood of a single donor.

[0047] In some embodiments, the composition may comprise one or more bioactive agents, including but not limited to cytokines or growth factors. The bioactive agents may include any of the following: chemokines, including macrophage inflammatory protein alpha (MIP-la), macrophage inflammatory protein beta (ΜΙΡ-Ιβ), and interferon gamma-induced protein 10 (IP-10), interleukins, including interleukin 1 Beta (IL-Ιβ), interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 6 (IL-6), interleukin 7 (IL-7), interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin 12 (IL-12), and interleukin 13 (IL-13), interferons, including interferon alpha (INFa), lymphokines, granulocyte

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PCT/US2018/034584 macrophage colony stimulating factor (GM-CSF), stem cell factor (KL), and tumor necrosis factor alpha (TNF-α). The bioactive agents may also include any of the following: vascular endothelial growth factor (VEGF), nerve growth factor, platelet-derived growth factor (PDGF), fibroblast growth factors (FGF), including fibroblast growth factor 2 (FGF-2), epidermal growth factor (EGF), keratinocyte growth factor, transforming growth factor (TGF), insulin-like growth factor, des(l-3)-IGF-I (brain IGF-I), neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF).

[0048] Other bioactive agents that may be incorporated into the composition may include one or more of the following: transforming growth factor Beta 1 (TGF-βΙ), transforming growth factor Alpha 1 (TGF-al), interleukin 16 (IL-16), platelet factor 4 (PF4), interferon gamma (IFNy), hepatocyte growth factor (HGF), insulin growth factor (IGF-1), fas ligand (Fas-L), monocyte chemoattractant protein 2 (MCP-2), monocyte chemoattractant protein 3 (MCP-3), monocyte chemoattractant protein 4 (MCP-4), macrophage derived chemokine (MDC), platelet factor 4 (PF-4), thrombopoietin (TPO), insulin-like growth factor 1 (IGF-1), and insulin-like growth factor binding protein-3 (IGFBP-3).

[0049] Other bioactive agents that may be incorporated into the composition may include one or more of the following: interleukin 15 (IL-15), interleukin 17 (IL-17), interleukin 18 (IL-18), interleukin 21 (IL-21), interleukin 22 (IL-22), interleukin 23 (IL-23), interleukin 27 (IL-27), interleukin 31 (IL-31), eotaxin, CXC ligand 1 (GROa), interleukin 1 receptor-alpha (IL-IRA), interleukin 1 alpha (IL-la), leukemia inhibitory factor (LIF), placental growth factor (PIGF, PLGF), chemokine ligand 5 (RANTES), stem cell factor (SCF), stromal cell derived factor (SDFla), and tumor necrosis factor (TNF β). Each Interleukin category may comprise one or more members within a category or family. For example, within the Interleukin 17 (IL-17) category, one of several species may be chosen, such as IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, or others. When IL-17 is generically denoted, it is intended to encompass any one or more of these different species.

[0050] In some embodiments, the composition may include albumin and one or more bioactive agents, wherein the albumin is derived from umbilical cord blood of a first donor, and the one or more bioactive agents are derived from at least a second donor. The one or more bioactive agents may be taken or derived from commercially available human albumin and/or commercially available human albumin serum. In some embodiments, the composition may include albumin and one or more bioactive agents, wherein the albumin and the one or more bioactive agents are derived from umbilical cord blood of a single donor.

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PCT/US2018/034584 [0051] In some embodiments, the composition may be produced such that it does not comprise dimethyl sulfoxide (DMSO) to avoid any potential harmful effects that DMSO may have, such as inducing allergic reactions in a subject. However, in some embodiments, DMSO may still be used, for example, as a cryoprotectant, as a replacement for Dextran, or in combination with Dextran. An alternative cryoprotectant or cryopreservative that may be substituted for Dextran and/or for DMSO, is CryoGold™, supplied by System Biosciences, Inc. of Palo Alto, CA, USA. Glycerol is another alternative cryoprotectant or cryopreservative that may be substituted.

[0052] A method of producing compositions such as the embodiments described herein is described according to a first embodiment. In step 10 of FIG. 1, a sample comprising human umbilical cord blood and having a first volume Vi is obtained. The human umbilical cord blood typically includes red blood cells, cells other than red blood cells, and non-cellular material. In step 12, at least about 95% of the red blood cells are removed from the sample and a substantial about of the non-cellular material is removed from the sample. Step 12 produces a pellet substantially comprising cells other than red blood cells. The pellet has a second volume V2. In some embodiments, at least about 98% of the red blood cells are removed from the sample. In some embodiments, at least about 99% of the red blood cells are removed from the sample. In some embodiments, at least about 95% (by volume) or the noncellular material is removed from the sample. In some embodiments, the removal of red blood cells and/or non-cellular material may comprise combining the human umbilical cord blood with PrepaCyte®-CB solution, Bio-E, LLC, Bloomington, MN, or an equivalent aggregation agent or sedimentation agent. In some embodiments, the removal of red blood cells and/or non-cellular material may comprise placing the human umbilical cord blood in a container within a centrifuge, and operating the centrifuge. This may occur after combining the human umbilical cord blood with PrepaCyte-CB solution, or an equivalent aggregation agent or sedimentation agent. In step 14, at least a portion of the pellet is reconstituted by diluting it with lactated Ringer’s solution. The at least a portion of pellet has an initial volume Vi, and the resulting diluted portion has a diluted volume Van. In some embodiments, a ratio Vdii/Vi between the diluted volume and the initial volume is between about 0.95 x V1/V2 and about 1.05 x V1/V2. In step 16, a container 30 (FIG. 2), which may comprise a tube configured for placement within a centrifuge, is filled with two layers. As shown in FIG. 2, a first layer 32 comprises a reagent that is configured for isolating mononuclear cells. In some embodiments, the reagent comprises Ficoll-Paque® Centrifugation Media, GE Healthcare Bio-Sciences AB, Uppsala, Sweden, or equivalent media. In some embodiments, the reagent

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PCT/US2018/034584 comprises Ficoll-Paque® PLUS Centrifugation Media. In some embodiments, the reagent comprises Ficoll-Paque® PREMIUM Centrifugation Media. In some embodiments, the reagent has a density of between about 1.0 grams/ml and about 1.3 grams/ml. In some embodiments, the reagent has a density of between about 1.07 grams/ml and about 1.09 grams/ml. In some embodiments, the reagent has a density of between about 1.073 grams/ml and about 1.084 grams/ml. A second layer 34 is placed over the first layer 32. The second layer 34 comprises at least a portion of the resulting diluted portion from step 14. The first layer 32 and second layer 34 are placed in the container 30, such that a volume V3 of the second layer 34 is placed over a volume V4 of the first layer 32, such that volume V4 is between about 15% and about 85% of V3. In some embodiments, volume V4 is between about 45% and about 55% of V3. In some embodiments, volume V4 is between about 75% and about 85% of V3. In some embodiments, volume V4 is about 50% of V3.

[0053] In step 18, a sufficient centrifugal force component 48 is placed on the container 30 (FIG. 3) and its contents (e.g., first layer 32 and second layer 34), with the centrifugal force component 48 directed toward a closed container end 50, to cause layering as shown in FIG. 4 to form. FIG. 3 illustrates the basic mechanics of a centrifuge 46 which spins the container 30 in a circular path 44 around a center point 42. In FIG. 4, a layer 36 comprises granulocytes and erythrocytes, and has migrated below a layer 32a containing the reagent which is configured for isolating mononuclear cells, and which remains from the first layer 32 of FIG. 2. Layer 38 substantially comprises mononuclear cells, and layer 40 substantially comprises plasma and platelets. In some embodiments, step 18 may be performed using a centrifuge which places at least about 350 times the acceleration due to gravity on the container 30 and its contents. In some embodiments, step 18 may be performed using a centrifuge which places at least about 400 times the acceleration due to gravity on the container 30 and its contents. In some embodiments, step 18 may be performed using a centrifuge which places at least about 450 times the acceleration due to gravity on the container 30 and its contents. In some embodiments, the centrifuge may be run between about 5 minutes and about 30 minutes to achieve the desired result. “Times the acceleration due to gravity” should be considered equivalent to relative centrifugal force (RCF).

[0054] In addition to controlling the amount of centrifugal force on the contents, the temperature on the contents, and/or the amount of time that the centrifuge is operated, a user may also choose to control the ramp up and ramp down (run profile) of the centrifuge; in other words, the time required to reach the chosen centrifugal force from a stopped condition, and the time required to reach a stopped condition from the nominally chosen centrifugal

WO 2018/226435

PCT/US2018/034584 force. In a traditional centrifuge, this corresponds to the increase of the rotational speed (e.g., RPM) from a stopped condition until a desired constant operation rate is reached that provides the desired relative centrifugal force, and the decrease of the rotational speed (from a desired constant operation rate) to a stopped condition (braking). This may also be referred to as a run profile. The start-up acceleration portion of the profile may be described as an acceleration curve, and the deceleration portion of the profile may be described as a braking curve. In certain centrifuges, for example, the acceleration may be controllable by a continuous adjustment or may have particular set points (e.g., 1, 2, 3, 4, 5, etc.). Additionally, deceleration (e.g., braking) may by controllable by continuous adjustment or may have particular set points (e.g., 1, 2, 3, 4, 5, etc.). The control of one or both of these two elements can be used to further optimize the effectiveness of the centrifugation. The time to ramp up to a desired centrifugal force from substantially zero centrifugal force may be between about 30 seconds and about 15 minutes, or between about one minute and about five minutes, or between about 10 seconds and 90 seconds. The time to ramp down from a desired centrifugal force to substantially zero centrifugal force may be between about 30 seconds and about 20 minutes, or between about one minute and about 10 minutes.

[0055] In step 20 mononuclear cells from the mononuclear cell layer 38 are removed from the container 30 and are combined with lactated Ringer’s solution, to create a mononuclear cell solution. Prior to the removal of the mononuclear cells from the container 30, the layer 40 may be first removed (FIG. 5), thus making it easier to remove the layer 38. Care can be taken so that the layer 38 is not disturbed when removing the layer 40, for example, by use of a sterile pipette. In step 22, the mononuclear cells are actively (though gently) mixed with the lactated Ringer’s solution. The mononuclear cells may also be washed one, two, or more times with lactated Ringer’s solution. In some embodiments, the mononuclear cell solution substantially comprises non-expanded cell populations. In some embodiments, the mononuclear cell solution comprises non-pooled cell populations. It should be noted that the layer 40 is substantially removed prior to removing the layer 38 to minimize any unnecessary contamination by platelets or plasma proteins. It should also be noted that the layer 32a is left substantially undisturbed when removing the layer 38, thus minimizing any unnecessary contamination by granulocytes.

[0056] In step 22, a volume Vs of either a portion of the mononuclear cell solution or substantially all of the mononuclear cell solution is used in order to estimate the number Nl of live cells in the volume Vs. In some embodiments, the estimating is done by performing a CD34+ count. In some embodiments, the estimating is done by performing a

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PCT/US2018/034584

Total Nucleated Cell (TNC) count. In some embodiments, the estimating is done at least in part with an automated cell counter. Alternative methods may be used for performing the cell count, including: a hemtocytometer following trypan blue staining, an ATP test, Calcein AM, a clonogenic assay, an ethidium homodimer assay, Evans blue, fluorescein diacetate hydrolysis/propidium iodide staining (FDA/PI staining), flow cytometry, formazan-basedassays (MTT/XTT), green fluorescent protein, lactate dehydrogenase (LDH), methyl violet, propidium iodide DNA stain (to differentiate necrotic, apoptotic, and normal cells), resazurin, trypan blue which only crosses cell membranes of dead cells, or TUNEL assay. At the end of step 22 substantially all of the lactated Ringer’s solution may be removed from the volume V5. In step 24, based at least partially upon the estimated number Nl of live cells, a calculated, estimated or determined volume Vr, of Low Molecular Weight Dextran in Dextrose solution is added to the volume V5 of the at least a portion of the mononuclear cell solution. In some embodiments, the calculation, estimation or determination of the volume Ve may be at least partially based on the assumption that approximately 75% of cells in the at least a portion mononuclear cell solution will be viable following freezing and thawing. In some embodiments, the calculation, estimation or determination of the volume Ve may be at least partially based on an intended live cell concentration of between about 2.5 million live cells and about 10.2 million live cells per milliliter. In some embodiments, the cells may be stored cryogenically. In some embodiments, the cryogenic storage is below about -60°C. In some embodiments, the cryogenic storage is below about -80°C. In some embodiments, the calculation, estimation or determination of the volume Ve may be at least partially based on an intended live cell concentration of between about 2.5 million live cells and about 10.2 million live cells per milliliter, and may assume that this live cell concentration will occur after adding albumin to the combined composition and cryogenically storing the combined composition. In some embodiments, the calculation, estimation or determination of the volume Ve may be at least partially based on an intended live cell concentration of between about 9.8 million live cells and about 10.2 million live cells per milliliter. In some embodiments, the Low Molecular Weight Dextran in Dextrose may comprise LMD (Dextran 40). In some embodiments, the volume Ve added corresponds to the equation

V₆ = K x Nl, [0057] where K is between about 7.35 χ IO'⁸ ml/cell and about 3.00 χ IO'⁷ml/cell, and [0058] where Nl is the number of live cells in a 20 ml portion of the mononuclear cell solution.

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PCT/US2018/034584 [0059] In some embodiments, the volume Vr, added corresponds to the equation

V₆ = K x Nl, [0060] where K is between about 7.35 χ IO'⁸ ml/cell and about 7.50 χ IO'⁸ml/cell, and [0061] where Nl is the number of live cells in a 20 ml portion of the mononuclear cell solution.

[0062] In step 26, the pH of the combined composition of the mononuclear cell solution and Low Molecular Weight Dextran in Dextrose solution may be adjusted if needed, in order to produce a pH within a desired range. In some embodiments, the pH is adjusted by adding a sodium bicarbonate, such as sodium bicarbonate powder. In some embodiments, the pH is adjusted by adding a calcium bicarbonate, such as calcium bicarbonate liquid. In some

embodiments,

the

pH

is

adjusted

to

or

maintained

at

between

4.1

and

8.5.

In

some

embodiments,

the

pH

is

adjusted

to

or

maintained

at

between

4.1

and

7.2.

In

some

embodiments,

the

pH

is

adjusted

to

or

maintained

at

between

4.1

and

6.2.

In

some

embodiments,

the

pH

is

adjusted

to

or

maintained

at

between

5.0

and

7.4.

In

some

embodiments,

the

pH

is

adjusted

to

or

maintained

at

between

5.0

and

7.2.

In

some

embodiments, the pH is adjusted to or maintained at between 5.0 and 6.2. In step 28, albumin is added to the combined composition. In some embodiments, between about 10 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 15 mg and about 30 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 18 mg and about 25 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, about 20 mg of albumin may be added per one milliliter of the combined composition.

[0063] In some embodiments, the mononuclear cell solution comprises stem cells. In some embodiments, the stem cells comprise hemapoietic stem cells (HSC). In some embodiments, the stem cells comprise mesenchymal stem cells (MSC).

[0064] In some embodiments, one or more bioactive agents may be added to the composition. The one or more bioactive agents may include cytokines or growth factors, and may be derived from human umbilical cord blood. In some embodiments, the one or more bioactive agents may be obtained or derived from the same sample comprising human umbilical cord blood that is described in step 10. In some embodiments, the one or more bioactive agents may be obtained or derived from human umbilical cord blood that is not the sample comprising human umbilical cord blood that is described in step 10. In some

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PCT/US2018/034584 embodiments, the one or more bioactive agents may be obtained or derived from human umbilical cord blood that is from a different donor than the sample comprising human umbilical cord blood that is described in step 10. In some embodiments, the one or more bioactive agents are obtained or derived from commercially available human albumin or commercially available human albumin serum.

[0065] In some embodiments, the one or more bioactive agents may include but are not limited to cytokines or growth factors. The bioactive agents may include any of the following: chemokines, including macrophage inflammatory protein alpha (MIP-la), macrophage inflammatory protein beta (ΜΙΡ-Ιβ), and interferon gamma-induced protein 10 (IP-10), interleukins, including interleukin 1 Beta (IL-Ιβ), interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 6 (IL-6), interleukin 7 (IL-7), interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin 12 (IL-12), and interleukin 13 (IL-13), interferons, including interferon alpha (INFa), lymphokines, granulocyte macrophage colony stimulating factor (GM-CSF), stem cell factor (KL), and tumor necrosis factor alpha (TNF-α). The bioactive agents may also include any of the following: vascular endothelial growth factor (VEGF), nerve growth factor, platelet-derived growth factor (PDGF), fibroblast growth factors (FGF), including fibroblast growth factor 2 (FGF-2), epidermal growth factor (EGF), keratinocyte growth factor, transforming growth factor (TGF), insulin-like growth factor, des(l-3)-IGF-I (brain IGF-I), neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF).

[0066] In some embodiments, dimethyl sulfoxide (DMSO) is not added into the composition in any of the steps, thus leaving the composition substantially DMSO-free, and to avoid any potential harmful effects that DMSO may have. The combined composition may then be cryogenically stored, for example, in cryogenic vials in an ultra-low freezer. The cryogenic storage may be controlled at a temperature below -60°C, or below -80°C.

[0067] A method of producing compositions such as the embodiments described herein is described according to a second embodiment. In step 100 of FIG. 6, a sample comprising human umbilical cord blood and having a first volume Vuc is obtained. The human umbilical cord blood typically includes red blood cells, cells other than red blood cells, and non-cellular material. In step 102, the sample comprising human umbilical cord blood is combined with a volume Vpc of PrepaCyte®-CB solution, Bio-E, LLC, Bloomington, MN, or an equivalent aggregation agent or sedimentation agent. Either or both of the volumes Vuc and Vpc may be selected such that the ratio Vuc/Vpc is between about 0.60 and about 1.75. In some embodiments, the ratio Vuc/Vpc is between about 0.75 and

WO 2018/226435

PCT/US2018/034584 about 1.25. In some embodiments, the ratio Vuc/Vpc is between about 0.60 and about 1.10. In some embodiments, the ratio Vuc/Vpc is between about 1.25 and about 1.75. The combined volumes Vuc and Vpc are allowed to stand undisturbed for at least about 20 minutes, or at least about 30 minutes, until a supernatant is formed, or until at least two distinct layers are formed. In step 104, a container 168 configured to be subjected to a centrifugal force (e.g., a centrifuge tube) is partially filled with a reagent 170 (FIG. 7) which is configured for isolating mononuclear cells, the reagent 170 having a volume Vr. In some embodiments, the reagent 170 comprises Ficoll-Paque® Centrifugation Media, GE Healthcare Bio-Sciences AB, Uppsala, Sweden, or equivalent media. In some embodiments, the reagent 170 comprises Ficoll-Paque® PLUS Centrifugation Media. In some embodiments, the reagent 170 comprises Ficoll-Paque® PREMIUM Centrifugation Media. In some embodiments, the reagent 170 has a density of between about 1.0 grams/ml and about 1.3 grams/ml. In some embodiments, the reagent 170 has a density of between about 1.07 grams/ml and about 1.09 grams/ml. In some embodiments, the reagent 170 has a density of between about 1.073 grams/ml and about 1.084 grams/ml. The volume Vr of the reagent 170 represents a first layer 172 (e.g., bottom layer) in the container 168. Further, as part of step 104, a second layer 174 comprising a volume Vs of the supernatant 176 produced in step 102 is layered over first layer 172 in the container 168, as shown in FIG. 7. The volume Vr of the layer 172 of the reagent 170 is at least 67% of the volume Vs of the layer 174 of the supernatant 176. In some embodiments, the volume Vr of the layer 172 of the reagent 170 is at least 75% of the volume Vs of the layer 174 of the supernatant 176. In some embodiments, the volume Vr of the layer 172 of the reagent 170 is at least 85% of the volume Vs of the layer 174 of the supernatant 176. In some embodiments, the volume Vr of the layer 172 of the reagent 170 is between about 30% and about 135% of the volume Vs of the layer 174 of the supernatant 176. In some embodiments, the volume Vr of the layer 172 of the reagent 170 is between about 50% and about 100% of the volume Vs of the layer 174 of the supernatant 176. In some embodiments, the volume Vr of the layer 172 of the reagent 170 is between about 75% and about 100% of the volume Vs of the layer 174 of the supernatant 176. The second layer 174 should be added over the first layer 172 in a slow or at least gentle or non-disruptive manner (e.g., slow speed pipetting) if a distinct interface between the two layers 172, 174 is initially desired.

[0068] In step 106, a sufficient centrifugal force component 178 is placed on the container 168 (FIG. 8) and its contents (e.g., first layer 172 and second layer 174), with the centrifugal force component 178 directed toward a closed container end 180, to cause

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PCT/US2018/034584 layering as shown in FIG. 9 to form. FIG. 8 illustrates the basic mechanics of a centrifuge 46 which spins the container 168 in a circular path 182 around a center point 184. The container 168 may, for example, be carefully placed within the centrifuge 46 in an appropriate location for spinning. In FIG. 9, a layer 186 comprises granulocytes and erythrocytes, and has migrated below a layer 172a containing the reagent 170, and which remains from the first layer 172 of FIG. 7. Layer 188 substantially comprises mononuclear cells, and layer 190 substantially comprises plasma and platelets. In some embodiments, step 106 may be performed using a centrifuge which places at least about 350 times the acceleration due to gravity on the container 168 and its contents. In some embodiments, step 106 may be performed using a centrifuge which places at least about 400 times the acceleration due to gravity on the container 168 and its contents. In some embodiments, step 106 may be performed using a centrifuge which places at least about 450 times the acceleration due to gravity on the container 168 and its contents. In some embodiments, step 106 may be performed using a centrifuge which places at least about 600 times the acceleration due to gravity on the container 168 and its contents. In some embodiments, step 106 may be performed using a centrifuge which places at least about 800 times the acceleration due to gravity on the container 168 and its contents. In some embodiments, step 106 may be performed using a centrifuge which places at least about 1,400 times the acceleration due to gravity on the container 168 and its contents. In some embodiments, the centrifuge may be run between about 5 minutes and about 30 minutes to achieve the desired result.

[0069] In addition to controlling the amount of centrifugal force on the contents, the temperature on the contents, and/or the amount of time that the centrifuge is operated, a user may also choose to control the ramp up and ramp down (run profile) of the centrifuge; in other words, the time required to reach the chosen centrifugal force from a stopped condition, and the time required to reach a stopped condition from the nominally chosen centrifugal force. In a traditional centrifuge, this corresponds to the increase of the rotational speed (e.g., RPM) from a stopped condition until a desired constant operation rate is reached that provides the desired relative centrifugal force, and the decrease of the rotational speed (from a desired constant operation rate) to a stopped condition (braking). This may also be referred to as a run profile. The start-up acceleration portion of the profile may be described as an acceleration curve, and the deceleration portion of the profile may be described as a braking curve. In certain centrifuges, for example, the acceleration may be controllable by a continuous adjustment or may have particular set points (e.g., 1, 2, 3, 4, 5, etc.). Additionally, deceleration (e.g., braking) may by controllable by continuous adjustment or may have

WO 2018/226435

PCT/US2018/034584 particular set points (e.g., 1, 2, 3, 4, 5, etc.). The control of one or both of these two elements can be used to further optimize the effectiveness of the centrifugation. The time to ramp up to a desired centrifugal force from substantially zero centrifugal force may be between about 30 seconds and about 15 minutes, or between about one minute and about five minutes, or between about 10 seconds and 90 seconds. The time to ramp down from a desired centrifugal force to substantially zero centrifugal force may be between about 30 seconds and about 20 minutes, or between about one minute and about 10 minutes.

[0070] In step 108, mononuclear cells from the mononuclear cell layer 188 are removed from the container 168 and are combined with lactated Ringer’s solution, to create a mononuclear cell solution. Prior to the removal of the mononuclear cells from the container 168, the layer 190 may be first removed (FIG. 10), thus making it easier to remove the layer 188. Care can be taken so that the layer 188 is not disturbed when removing the layer 190, for example, by use of a sterile pipette. In step 108, the mononuclear cells from the mononuclear cell layer 188 are actively (though gently) mixed with the lactated Ringer’s solution. The mononuclear cells may also be washed one, two, or more times with lactated Ringer’s solution. In some embodiments, the mononuclear cell solution substantially comprises nonexpanded cell populations. In some embodiments, the mononuclear cell solution comprises non-pooled cell populations. It should be noted that the layer 190 is substantially removed prior to removing the mononuclear cells from the layer 188 to minimize any unnecessary contamination by platelets or plasma proteins. It should also be noted that the layer 172a is left substantially undisturbed when removing the layer 188, thus minimizing any unnecessary contamination by the granulocytes in layer 186.

[0071] In step 110, a volume Vm of either a portion of the mononuclear cell solution or substantially all of the mononuclear cell solution is used in order to estimate the number Nl of live cells in the volume Vm. In some embodiments, the estimating is done by performing a CD34+ count. In some embodiments, the estimating is done by performing a Total Nucleated Cell (TNC) count. In some embodiments, the estimating is done at least in part with an automated cell counter. Alternative methods may be used for performing the cell count, including: a hemtocytometer following trypan blue staining, an ATP test, Calcein AM, a clonogenic assay, an ethidium homodimer assay, Evans blue, fluorescein diacetate hydrolysis/propidium iodide staining (FDA/PI staining), flow cytometry, formazan-basedassays (MTT/XTT), green fluorescent protein, lactate dehydrogenase (LDH), methyl violet, propidium iodide DNA stain (to differentiate necrotic, apoptotic, and normal cells), resazurin, trypan blue which only crosses cell membranes of dead cells, or TUNEL assay. At the end of

WO 2018/226435

PCT/US2018/034584 step 110 substantially all of the lactated Ringer’s solution may be removed from the volume V_M.

[0072] In step 112, albumin is added to the combined composition. In some embodiments, between about 10 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 10 mg and about 120 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 50 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 50 mg and about 120 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 80 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 80 mg and about 120 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 105 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 105 mg and about 120 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 15 mg and about 30 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 18 mg and about 25 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, about 20 mg of albumin may be added per one milliliter of the combined composition.

[0073] In some embodiments, Low Molecular Weight Dextran in Dextrose solution is added to the volume Vm of the at least a portion of the mononuclear cell solution during or prior to the addition of albumin. In some embodiments, the amount of Low Molecular Weight Dextran in Dextrose solution is based at least partially upon the estimated number Nl of live cells, a calculated, estimated or determined. In some embodiments, the calculation, estimation or determination of the volume Vdd pf the Low Molecular Weight Dextran in Dextrose solution added may be at least partially based on the assumption that approximately 75% of cells in the at least a portion mononuclear cell solution will be viable following freezing and thawing. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 100,000 live cells and about 75 million live cells per milliliter. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 750,000 live cells and about 30 million live cells per milliliter. In some embodiments, the

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PCT/US2018/034584 cells may be stored cryogenically. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 10 million live cells and about 30 million live cells per milliliter. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 10 million live cells and about 20 million live cells per milliliter. In some embodiments, the cryogenic storage is below about -60°C. In some embodiments, the cryogenic storage is below about -80°C.

[0074] In some embodiments, the pH of the combined composition may be adjusted if needed, in order to produce a pH within a desired range. In some embodiments, the pH is adjusted by adding a sodium bicarbonate, such as sodium bicarbonate powder. In some embodiments, the pH is adjusted by adding a calcium bicarbonate, such as calcium bicarbonate solution. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 8.5. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 7.2. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 6.2. In some embodiments, the pH is adjusted to or maintained at between 5.0 and 7.4. In some embodiments, the pH is adjusted to or maintained at between 5.0 and 7.2. In some embodiments, the pH is adjusted to or maintained at between 5.0 and 6.2.

[0075] In some embodiments, the mononuclear cell solution comprises stem cells. In some embodiments, the stem cells comprise hemapoietic stem cells (HSC). In some embodiments, the stem cells comprise mesenchymal stem cells (MSC).

[0076] In some embodiments, one or more bioactive agents may be added to the composition. The one or more bioactive agents may include cytokines or growth factors, and may be derived from human umbilical cord blood. In some embodiments, the one or more bioactive agents may be obtained or derived from the same sample comprising human umbilical cord blood that is described in step 100. In some embodiments, the one or more bioactive agents may be obtained or derived from human umbilical cord blood that is not the sample comprising human umbilical cord blood that is described in step 100. In some embodiments, the one or more bioactive agents may be obtained or derived from human umbilical cord blood that is from a different donor than the sample comprising human umbilical cord blood that is described in step 100. In some embodiments, the one or more bioactive agents are obtained or derived from commercially available human albumin or commercially available human albumin serum.

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PCT/US2018/034584 [0077] In some embodiments, the one or more bioactive agents may include but are not limited to cytokines or growth factors. The bioactive agents may include any of the following: chemokines, including macrophage inflammatory protein alpha (MIP-la), macrophage inflammatory protein beta (ΜΙΡ-Ιβ), interferon gamma-induced protein 10 (IP10), interleukins, including interleukin 1 Beta (IL-Ιβ), interleukin 2 (IL-2), interleukin 4 (IL4), interleukin 5 (IL-5), interleukin 6 (IL-6), interleukin 7 (IL-7), interleukin 8 (IL-8), interleukin 9 (IL-9), interleukin 10 (IL-10), interleukin 12 (IL-12), interleukin 13 (IL-13), interleukin 15 (IL-15), interleukin 17 (IL-17), interleukin 18 (IL-18), interleukin 21 (IL-21), interleukin 22 (IL-22), interleukin 23 (IL-23), interleukin 27 (IL-27), interleukin 31 (IL-31), interferons, including interferon alpha (INFa), a lymphokine, granulocyte macrophage colony stimulating factor (GM-CSF), stem cell factor (KL), tumor necrosis factor alpha (TNF-a), vascular endothelial growth factor (VEGF), nerve growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor 2 (FGF-2), epidermal growth factor (EGF), keratinocyte growth factor, transforming growth factor (TGF), insulin-like growth factor, des(l-3)-IGF-I (brain IGF-I), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), eotaxin, CXC ligand 1 (GROa), interleukin 1 receptor-alpha (IL-IRA), interleukin 1 alpha (IL-1 a), leukemia inhibitory factor (LIF), placental growth factor (PIGF, PLGF), chemokine ligand 5 (RANTES), stem cell factor (SCF), stromal cell derived factor (SDFla), and tumor necrosis factor (TNF β).

[0078] In some embodiments, dimethyl sulfoxide (DMSO) is not added into the composition in any of the steps, thus leaving the composition substantially DMSO-free, and to avoid any potential harmful effects that DMSO may have. The combined composition may then be cryogenically stored, for example, in cryogenic vials in an ultra-low freezer. The cryogenic storage may be controlled at a temperature below -60°C, or below -80°C.

[0079] A method of producing compositions such as the embodiments described herein is described according to a third embodiment. In step 200 of FIG. 11, a sample comprising human umbilical cord blood and having a first volume Vuc is obtained. The human umbilical cord blood typically includes red blood cells, cells other than red blood cells, and non-cellular material. In step 202, the sample comprising human umbilical cord blood 271 is mixed with a volume Vpc of PrepaCyte®-CB solution 273, Bio-E, LLC, Bloomington, MN, or an equivalent aggregation agent or sedimentation agent, within a first flexible container 267, as shown in FIG. 12. A spike 277 and insertion tube 279 connected to the first flexible container 267 may be utilized to insert the sample comprising human umbilical cord blood 271, or even the volume Vpc of PrepaCyte®-CB solution 273. In some

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PCT/US2018/034584 embodiments, the volume Vpc of PrepaCyte®-CB solution 273 is received already within the first flexible container 267. Either or both of the volumes Vuc and Vpc may be selected such that the ratio Vuc/Vpc is between about 0.60 and about 1.75. In some embodiments, the ratio Vuc/Vpc is between about 0.75 and about 1.25. In some embodiments, the ratio Vuc/Vpc is between about 0.60 and about 1.10. In some embodiments, the ratio Vuc/Vpc is between about 1.25 and about 1.75. In step 204, the mixed volumes Vuc + Vpc are allowed to remain in the first flexible container for at least 20 minutes, until a supernatant 275 is formed. In some embodiments, the mixed volumes Vuc + Vpc are allowed to remain in the first flexible container 267 for at least 30 minutes. In step 206, at least a portion of the supernatant 275 is forced from the from the first flexible container 267 into a second flexible container 268 (arrow) via a conduit 269 which links the first flexible container 267 to the second flexible container 268, as shown in FIG. 14. In step 208, the second flexible container 268 is placed in a centrifuge and the centrifuge is operated to apply a centrifugal force on the supernatant within the second flexible container 268. In some embodiments, the first flexible container 267 may be removed from the second flexible container 268 and discarded, for example, by cutting the conduit 269. The portion of the cut conduit 269 extending from the second flexible container 268 may be clamped off after cutting. A sufficient centrifugal force component 278 is placed on the second flexible container 268 (FIG. 13) and its contents, with the centrifugal force component 278 directed toward a closed container end 280, to cause layering as shown in FIG. 15 to form. FIG. 13 illustrates the basic mechanics of a centrifuge 46 which spins the second flexible container 268 in a circular path 282 around a center point 284. The second flexible container 268 may, for example, be carefully placed within the centrifuge 46 in an appropriate location for spinning. In FIG. 15, a layer 286 comprises granulocytes and erythrocytes and a layer 272a contains the remaining PrepaCyte®-CB solution 273. Layer 288 substantially comprises mononuclear cells, and layer 290 substantially comprises plasma and platelets. In some embodiments, step 208 may be performed using a centrifuge which places at least about 350 times the acceleration due to gravity on the second flexible container 268 and its contents. In some embodiments, step 208 may be performed using a centrifuge which places at least about 400 times the acceleration due to gravity on the second flexible container 268 and its contents. In some embodiments, step 208 may be performed using a centrifuge which places at least about 450 times the acceleration due to gravity on the second flexible container 268 and its contents. In some embodiments, step 208 may be performed using a centrifuge which places at least about 600 times the acceleration due to gravity on the second flexible container 268 and its contents. In

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PCT/US2018/034584 some embodiments, step 208 may be performed using a centrifuge which places at least about 800 times the acceleration due to gravity on the second flexible container 268 and its contents. In some embodiments, step 208 may be performed using a centrifuge which places at least about 1,400 times the acceleration due to gravity on the container 268 and its contents. In some embodiments, the centrifuge may be run between about 5 minutes and about 30 minutes to achieve the desired result.

[0080] In addition to controlling the amount of centrifugal force on the contents, the temperature on the contents, and/or the amount of time that the centrifuge is operated, a user may also choose to control the ramp up and ramp down (run profile) of the centrifuge; in other words, the time required to reach the chosen centrifugal force from a stopped condition, and the time required to reach a stopped condition from the nominally chosen centrifugal force. In a traditional centrifuge, this corresponds to the increase of the rotational speed (e.g., RPM) from a stopped condition until a desired constant operation rate is reached that provides the desired relative centrifugal force, and the decrease of the rotational speed (from a desired constant operation rate) to a stopped condition (braking). This may also be referred to as a run profile. The start-up acceleration portion of the profile may be described as an acceleration curve, and the deceleration portion of the profile may be described as a braking curve. In certain centrifuges, for example, the acceleration may be controllable by a continuous adjustment or may have particular set points (e.g., 1, 2, 3, 4, 5, etc.). Additionally, deceleration (e.g., braking) may by controllable by continuous adjustment or may have particular set points (e.g., 1, 2, 3, 4, 5, etc.). The control of one or both of these two elements can be used to further optimize the effectiveness of the centrifugation. The time to ramp up to a desired centrifugal force from substantially zero centrifugal force may be between about 30 seconds and about 15 minutes, or between about one minute and about five minutes, or between about 10 seconds and 90 seconds. The time to ramp down from a desired centrifugal force to substantially zero centrifugal force may be between about 30 seconds and about 20 minutes, or between about one minute and about 10 minutes.

[0081] In step 210, mononuclear cells from the mononuclear cell layer 288 are removed from the second flexible container 268 and are combined with a reagent to create a mononuclear cell solution. In some embodiments, the reagent comprises human serum, for example, 5% human serum albumin. Prior to the removal of the mononuclear cells from the second flexible container 268, the layer 290 may be first removed, thus making it easier to remove the layer 288. Care can be taken so that the layer 288 is not disturbed when removing the layer 290, for example, by use of a sterile pipette. The layer 290 may also be carefully

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PCT/US2018/034584 removed (e.g. by aspiration with a needle) through an additional access tube 281 having a port or valve 283. In some embodiments, the mononuclear cell solution substantially comprises non-expanded cell populations. In some embodiments, the mononuclear cell solution comprises non-pooled cell populations. It should be noted that the layer 290 is substantially removed prior to removing the mononuclear cells from the layer 288 to minimize any unnecessary contamination by platelets or plasma proteins. It should also be noted that the layer 272a is left substantially undisturbed when removing the layer 288, thus minimizing any unnecessary contamination by the granulocytes in layer 286.

[0082] In step 212, a volume Vm of either a portion of the mononuclear cell solution or substantially all of the mononuclear cell solution is used in order to estimate the number Nl of live cells in the volume Vm. In some embodiments, the estimating is done by performing a CD34+ count. In some embodiments, the estimating is done by performing a Total Nucleated Cell (TNC) count. In some embodiments, the estimating is done at least in part with an automated cell counter. Alternative methods may be used for performing the cell count, including: a hemtocytometer following trypan blue staining, an ATP test, Calcein AM, a clonogenic assay, an ethidium homodimer assay, Evans blue, fluorescein diacetate hydrolysis/propidium iodide staining (FDA/PI staining), flow cytometry, formazan-basedassays (MTT/XTT), green fluorescent protein, lactate dehydrogenase (LDH), methyl violet, propidium iodide DNA stain (to differentiate necrotic, apoptotic, and normal cells), resazurin, trypan blue which only crosses cell membranes of dead cells, or TUNEL assay. At the end of step 212 substantially all of the reagent may be removed from the volume Vm.

[0083] In step 214, albumin is added to the combined composition. In some embodiments, between about 10 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 10 mg and about 120 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 50 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 50 mg and about 120 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 80 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 80 mg and about 120 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 105 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 105 mg and about 120 mg of albumin may be added per one milliliter of the combined composition.

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In some embodiments, between about 15 mg and about 30 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 18 mg and about 25 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, about 20 mg of albumin may be added per one milliliter of the combined composition.

[0084] In some embodiments, Low Molecular Weight Dextran in Dextrose solution is added to the volume Vm of the at least a portion of the mononuclear cell solution during or prior to the addition of albumin. In some embodiments, the amount of Low Molecular Weight Dextran in Dextrose solution is based at least partially upon the estimated number Nl of live cells, a calculated, estimated or determined. In some embodiments, the calculation, estimation or determination of the volume Vdd pf the Low Molecular Weight Dextran in Dextrose solution added may be at least partially based on the assumption that approximately 75% of cells in the at least a portion mononuclear cell solution will be viable following freezing and thawing. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 100,000 live cells and about 75 million live cells per milliliter. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 750,000 live cells and about 30 million live cells per milliliter. In some embodiments, the cells may be stored cryogenically. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 10 million live cells and about 30 million live cells per milliliter. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 10 million live cells and about 20 million live cells per milliliter. In some embodiments, the cryogenic storage is below about -60°C. In some embodiments, the cryogenic storage is below about -80°C.

[0085] In some embodiments, the pH of the combined composition may be adjusted if needed, in order to produce a pH within a desired range. In some embodiments, the pH is adjusted by adding a sodium bicarbonate, such as sodium bicarbonate powder. In some embodiments, the pH is adjusted by adding a calcium bicarbonate, such as calcium bicarbonate solution. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 8.5. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 7.2. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 6.2. In

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PCT/US2018/034584 some embodiments, the pH is adjusted to or maintained at between 5.0 and 7.4. In some embodiments, the pH is adjusted to or maintained at between 5.0 and 7.2. In some embodiments, the pH is adjusted to or maintained at between 5.0 and 6.2.

[0086] In some embodiments, the mononuclear cell solution comprises stem cells. In some embodiments, the stem cells comprise hemapoietic stem cells (HSC). In some embodiments, the stem cells comprise mesenchymal stem cells (MSC).

[0087] In some embodiments, one or more bioactive agents may be added to the composition. The one or more bioactive agents may include cytokines or growth factors, and may be derived from human umbilical cord blood. In some embodiments, the one or more bioactive agents may be obtained or derived from the same sample comprising human umbilical cord blood that is described in step 200. In some embodiments, the one or more bioactive agents may be obtained or derived from human umbilical cord blood that is not the sample comprising human umbilical cord blood that is described in step 200. In some embodiments, the one or more bioactive agents may be obtained or derived from human umbilical cord blood that is from a different donor than the sample comprising human umbilical cord blood that is described in step 200. In some embodiments, the one or more bioactive agents are obtained or derived from commercially available human albumin or commercially available human albumin serum.

[0088] In some embodiments, the one or more bioactive agents may include but are not limited to cytokines or growth factors. The bioactive agents may include any of the following: chemokines, including macrophage inflammatory protein alpha (MIP-la), macrophage inflammatory protein beta (ΜΙΡ-Ιβ), interferon gamma-induced protein 10 (IP10), interleukins, including interleukin 1 Beta (ZL-Ιβ), interleukin 2 (IL-2), interleukin 4 (IL4), interleukin 5 (IL-5), interleukin 6 (IL-6), interleukin 7 (IL-7), interleukin 8 (IL-8), interleukin 9 (IL-9), interleukin 10 (IL-10), interleukin 12 (IL-12), interleukin 13 (IL-13), interleukin 15 (IL-15), interleukin 17 (IL-17), interleukin 18 (IL-18), interleukin 21 (IL-21), interleukin 22 (IL-22), interleukin 23 (IL-23), interleukin 27 (IL-27), interleukin 31 (IL-31), interferons, including interferon alpha (INFa), a lymphokine, granulocyte macrophage colony stimulating factor (GM-CSF), stem cell factor (KL), tumor necrosis factor alpha (TNF-a), vascular endothelial growth factor (VEGF), nerve growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor 2 (FGF-2), epidermal growth factor (EGF), keratinocyte growth factor, transforming growth factor (TGF), insulin-like growth factor, des(l-3)-IGF-I (brain IGF-I), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), eotaxin, CXC ligand 1 (GROa), interleukin 1 receptor-alpha (IL-IRA), interleukin

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PCT/US2018/034584 alpha (IL-1 a), leukemia inhibitory factor (LIF), placental growth factor (PIGF, PLGF), chemokine ligand 5 (RANTES), stem cell factor (SCF), stromal cell derived factor (SDFla), and tumor necrosis factor (TNF β).

[0089] In some embodiments, dimethyl sulfoxide (DMSO) is not added into the composition in any of the steps, thus leaving the composition substantially DMSO-free, and to avoid any potential harmful effects that DMSO may have. The combined composition may then be cryogenically stored, for example, in cryogenic vials in an ultra-low freezer. The cryogenic storage may be controlled at a temperature below -60°C, or below -80°C.

[0090] A method of producing compositions such as the embodiments described herein is described according to a fourth embodiment. In step 300 of FIG. 16, a sample comprising human umbilical cord blood and having a first volume Vuc is obtained. The human umbilical cord blood typically includes red blood cells, cells other than red blood cells, and non-cellular material. In step 302, the sample comprising human umbilical cord blood is combined with a volume Vpc of PrepaCyte®-CB solution, Bio-E, LLC, Bloomington, MN, or an equivalent aggregation agent or sedimentation agent. Either of both of the volumes Vuc and Vpc may be selected such that the ratio Vuc/Vpc is between about 0.60 and about 1.75. In some embodiments, the ratio Vuc/Vpc is between about 0.75 and about 1.25. In some embodiments, the ratio Vuc/Vpc is between about 0.60 and about 1.10. In some embodiments, the ratio Vuc/Vpc is between about 1.25 and about 1.75. The combined volumes Vuc and Vpc are allowed to stand undisturbed for at least about 20 minutes, or at least about 30 minutes, until a supernatant is formed, or until at least two distinct layers are formed. In step 304, a container 368 configured to be subjected to a centrifugal force (e.g., a centrifuge tube) is partially filled with a reagent 370 (FIG. 17) which is configured for isolating mononuclear cells, the reagent 370 having a volume Vr. In some embodiments, the reagent 370 comprises Ficoll-Paque® Centrifugation Media, GE Healthcare Bio-Sciences AB, Uppsala, Sweden, or equivalent media. In some embodiments, the reagent 370 comprises Ficoll-Paque® PLUS Centrifugation Media. In some embodiments, the reagent 370 comprises Ficoll-Paque® PREMIUM Centrifugation Media. In some embodiments, the reagent 370 has a density of between about 1.0 grams/ml and about 1.3 grams/ml. In some embodiments, the reagent 370 has a density of between about 1.07 grams/ml and about 1.09 grams/ml. In some embodiments, the reagent 370 has a density of between about 1.073 grams/ml and about 1.084 grams/ml. The volume Vr of the reagent 370 represents a first layer 372 (e.g., bottom layer) in the container 368. Further, as part of step 304, a second layer 374 comprising a volume Vs of the supernatant 376 produced in step 302 is layered directly

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PCT/US2018/034584 over first layer 372 in the container 368, as shown in FIG. 17. The layer 372 of the reagent 370 is layered with a height, or column height CHr, that is at least 67% of the height, or column height CHs, of the layer 374 of the supernatant 376. In some embodiments, the column height CHr of the layer 372 of the reagent 370 is at least 75% of the column height CHs of the layer 374 of the supernatant 376. In some embodiments, the column height CHr of the layer 372 of the reagent 370 is at least 85% of the column height CHs of the layer 374 of the supernatant 376. In some embodiments, the column height CHr of the layer 372 of the reagent 370 is between about 30% and about 135% of the column height CHs of the layer 374 of the supernatant 376. In some embodiments, the column height CHr of the layer 372 of the reagent 370 is between about 50% and about 100% of the column height CHs of the layer 374 of the supernatant 376. In some embodiments, the column height CHr of the layer 372 of the reagent 370 is between about 75% and about 100% of the column height CHs of the layer 374 of the supernatant 376. The second layer 374 should be added over the first layer 372 in a slow or at least gentle or non-disruptive manner (e.g., slow speed pipetting) if a distinct interface between the two layers 372, 374 is initially desired.

[0091] In step 306, a sufficient centrifugal force component 378 is placed on the container 368 (FIG. 18) and its contents (e.g., first layer 372 and second layer 374), with the centrifugal force component 378 directed toward a closed container end 380, to cause layering as shown in FIG. 19 to form. FIG. 18 illustrates the basic mechanics of a centrifuge 46 which spins the container 368 in a circular path 382 around a center point 384. The container 368 may, for example, be carefully placed within the centrifuge 46 in an appropriate location for spinning. In FIG. 19, a layer 386 comprises granulocytes and erythrocytes, and has migrated below a layer 372a containing the reagent 370, and which remains from the first layer 372 of FIG. 17. Layer 388 substantially comprises mononuclear cells, and layer 390 substantially comprises plasma and platelets. In some embodiments, step 306 may be performed using a centrifuge which places at least about 350 times the acceleration due to gravity on the container 368 and its contents. In some embodiments, step 306 may be performed using a centrifuge which places at least about 400 times the acceleration due to gravity on the container 368 and its contents. In some embodiments, step 306 may be performed using a centrifuge which places at least about 450 times the acceleration due to gravity on the container 368 and its contents. In some embodiments, step 306 may be performed using a centrifuge which places at least about 600 times the acceleration due to gravity on the container 368 and its contents. In some embodiments, step 306 may be performed using a centrifuge which places at least about 800 times the

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PCT/US2018/034584 acceleration due to gravity on the container 368 and its contents. In some embodiments, step 306 may be performed using a centrifuge which places at least about 1,400 times the acceleration due to gravity on the container 368 and its contents. In some embodiments, the centrifuge may be run between about 5 minutes and about 30 minutes to achieve the desired result. In one embodiment, step 406 is performed using a centrifuge which places between about 750 times the acceleration due to gravity to about 900 times the acceleration due to gravity on the container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 16°C and 22°C. In another embodiment, step 406 is performed using a centrifuge which places between about 800 times the acceleration due to gravity to about 850 times the acceleration due to gravity on the container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 16°C and 22°C. In another embodiment, step 406 is performed using a centrifuge which places between about 750 times the acceleration due to gravity to about 900 times the acceleration due to gravity on the container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 17°C and 21 °C. In another embodiment, step 406 is performed using a centrifuge which places between about 800 times the acceleration due to gravity to about 850 times the acceleration due to gravity on the container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 17°C and 21 °C.

[0092] In addition to controlling the amount of centrifugal force on the contents, the temperature on the contents, and/or the amount of time that the centrifuge is operated, a user may also choose to control the ramp up and ramp down (run profile) of the centrifuge; in other words, the time required to reach the chosen centrifugal force from a stopped condition, and the time required to reach a stopped condition from the nominally chosen centrifugal force. In a traditional centrifuge, this corresponds to the increase of the rotational speed (e.g., RPM) from a stopped condition until a desired constant operation rate is reached that provides the desired relative centrifugal force, and the decrease of the rotational speed (from a desired constant operation rate) to a stopped condition (braking). This may also be referred to as a run profile. The start-up acceleration portion of the profile may be described as an acceleration curve, and the deceleration portion of the profile may be described as a braking curve. In certain centrifuges, for example, the acceleration may be controllable by a continuous adjustment or may have particular set points (e.g., 1, 2, 3, 4, 5, etc.). Additionally, deceleration (e.g., braking) may by controllable by continuous adjustment or may have

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[0093] In step 308, mononuclear cells from the mononuclear cell layer 388 are removed from the container 368 and are combined with lactated Ringer’s solution, to create a mononuclear cell solution. Prior to the removal of the mononuclear cells from the container 368, the layer 390 may be first removed (FIG. 20), thus making it easier to remove the layer 388. Care can be taken so that the layer 388 is not disturbed when removing the layer 390, for example, by use of a sterile pipette. In step 308, the mononuclear cells from the mononuclear cell layer 388 are actively (though gently) mixed with the lactated Ringer’s solution. The mononuclear cells may also be washed one, two, or more times with lactated Ringer’s solution. In some embodiments, the mononuclear cell solution substantially comprises nonexpanded cell populations. In some embodiments, the mononuclear cell solution comprises non-pooled cell populations. It should be noted that the layer 390 is substantially removed prior to removing the mononuclear cells from the layer 388 to minimize any unnecessary contamination by platelets or plasma proteins. It should also be noted that the layer 372a is left substantially undisturbed when removing the layer 388, thus minimizing any unnecessary contamination by the granulocytes in layer 386.

[0094] In step 310, a volume Vm of either a portion of the mononuclear cell solution or substantially all of the mononuclear cell solution is used in order to estimate the number Nl of live cells in the volume Vm. In some embodiments, the estimating is done by performing a CD34+ count. In some embodiments, the estimating is done by performing a Total Nucleated Cell (TNC) count. In some embodiments, the estimating is done at least in part with an automated cell counter. Alternative methods may be used for performing the cell count, including: a hemtocytometer following trypan blue staining, an ATP test, Calcein AM, a clonogenic assay, an ethidium homodimer assay, Evans blue, fluorescein diacetate hydrolysis/propidium iodide staining (FDA/PI staining), flow cytometry, formazan-basedassays (MTT/XTT), green fluorescent protein, lactate dehydrogenase (LDH), methyl violet, propidium iodide DNA stain (to differentiate necrotic, apoptotic, and normal cells), resazurin, trypan blue which only crosses cell membranes of dead cells, or TUNEL assay. At the end of

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PCT/US2018/034584 step 310 substantially all of the lactated Ringer’s solution may be removed from the volume V_M.

[0095] In step 312, albumin is added to the combined composition. In some embodiments, between about 10 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 10 mg and about 120 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 50 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 50 mg and about 120 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 80 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 80 mg and about 120 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 105 mg and about 150 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 105 mg and about 120 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 15 mg and about 30 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, between about 18 mg and about 25 mg of albumin may be added per one milliliter of the combined composition. In some embodiments, about 20 mg of albumin may be added per one milliliter of the combined composition.

[0096] In some embodiments, Low Molecular Weight Dextran in Dextrose solution is added to the volume Vm of the at least a portion of the mononuclear cell solution during or prior to the addition of albumin. In some embodiments, the amount of Low Molecular Weight Dextran in Dextrose solution is based at least partially upon the estimated number Nl of live cells, a calculated, estimated or determined. In some embodiments, the calculation, estimation or determination of the volume Vdd pf the Low Molecular Weight Dextran in Dextrose solution added may be at least partially based on the assumption that approximately 75% of cells in the at least a portion mononuclear cell solution will be viable following freezing and thawing. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 100,000 live cells and about 75 million live cells per milliliter. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 750,000 live cells and about 30 million live cells per milliliter. In some embodiments, the

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[0097] In some embodiments, the pH of the combined composition may be adjusted if needed, in order to produce a pH within a desired range. In some embodiments, the pH is adjusted by adding a sodium bicarbonate, such as sodium bicarbonate powder. In some embodiments, the pH is adjusted by adding a calcium bicarbonate, such as calcium bicarbonate solution. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 8.5. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 7.2. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 6.2. In some embodiments, the pH is adjusted to or maintained at between 5.0 and 7.4. In some embodiments, the pH is adjusted to or maintained at between 5.0 and 7.2. In some embodiments, the pH is adjusted to or maintained at between 5.0 and 6.2.

[0098] In some embodiments, the mononuclear cell solution comprises stem cells. In some embodiments, the stem cells comprise hemapoietic stem cells (HSC). In some embodiments, the stem cells comprise mesenchymal stem cells (MSC).

[0099] In some embodiments, one or more bioactive agents may be added to the composition. The one or more bioactive agents may include cytokines or growth factors, and may be derived from human umbilical cord blood. In some embodiments, the one or more bioactive agents may be obtained or derived from the same sample comprising human umbilical cord blood that is described in step 300. In some embodiments, the one or more bioactive agents may be obtained or derived from human umbilical cord blood that is not the sample comprising human umbilical cord blood that is described in step 300. In some embodiments, the one or more bioactive agents may be obtained or derived from human umbilical cord blood that is from a different donor than the sample comprising human umbilical cord blood that is described in step 300. In some embodiments, the one or more bioactive agents are obtained or derived from commercially available human albumin or commercially available human albumin serum.

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PCT/US2018/034584 [0100] In some embodiments, the one or more bioactive agents may include but are not limited to cytokines or growth factors. The bioactive agents may include any of the following: chemokines, including macrophage inflammatory protein alpha (MIP-la), macrophage inflammatory protein beta (ΜΙΡ-Ιβ), interferon gamma-induced protein 10 (IP10), interleukins, including interleukin 1 Beta (IL-Ιβ), interleukin 2 (IL-2), interleukin 4 (IL4), interleukin 5 (IL-5), interleukin 6 (IL-6), interleukin 7 (IL-7), interleukin 8 (IL-8), interleukin 9 (IL-9), interleukin 10 (IL-10), interleukin 12 (IL-12), interleukin 13 (IL-13), interleukin 15 (IL-15), interleukin 17 (IL-17), interleukin 18 (IL-18), interleukin 21 (IL-21), interleukin 22 (IL-22), interleukin 23 (IL-23), interleukin 27 (IL-27), interleukin 31 (IL-31), interferons, including interferon alpha (INFa), a lymphokine, granulocyte macrophage colony stimulating factor (GM-CSF), stem cell factor (KL), tumor necrosis factor alpha (TNF-a), vascular endothelial growth factor (VEGF), nerve growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor 2 (FGF-2), epidermal growth factor (EGF), keratinocyte growth factor, transforming growth factor (TGF), insulin-like growth factor, des(l-3)-IGF-I (brain IGF-I), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), eotaxin, CXC ligand 1 (GROa), interleukin 1 receptor-alpha (IL-IRA), interleukin 1 alpha (IL-1 a), leukemia inhibitory factor (LIF), placental growth factor (PIGF, PLGF), chemokine ligand 5 (RANTES), stem cell factor (SCF), stromal cell derived factor (SDFla), and tumor necrosis factor (TNF β).

[0101] In some embodiments, dimethyl sulfoxide (DMSO) is not added into the composition in any of the steps, thus leaving the composition substantially DMSO-free, and to avoid any potential harmful effects that DMSO may have. The combined composition may then be cryogenically stored, for example, in cryogenic vials in an ultra-low freezer. The cryogenic storage may be controlled at a temperature below -60°C, or below -80°C.

[0102] A method of producing compositions such as the embodiments described herein is described according to a fifth embodiment. In step 400 of FIG. 21, a sample comprising human umbilical cord blood and having a first volume Vuc is obtained. The human umbilical cord blood typically includes red blood cells, cells other than red blood cells, and non-cellular material. In step 402, the sample comprising human umbilical cord blood is combined with a volume Vpc of PrepaCyte®-CB solution, Bio-E, LLC, Bloomington, MN, or an equivalent aggregation agent or sedimentation agent. Either or both of the volumes Vuc and Vpc may be selected such that the ratio Vuc/Vpc is between about 0.60 and about 1.75. In some embodiments, the ratio Vuc/Vpc is between about 0.75 and about 1.25. In some embodiments, the ratio Vuc/Vpc is between about 0.60 and about 1.10.

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The combined volumes Vuc and Vpc are allowed to stand undisturbed for at least about 20 minutes, or at least about 30 minutes, until a supernatant is formed, or until at least two distinct layers are formed. In step 404, a container 468 configured to be subjected to a centrifugal force (e.g., a centrifuge tube) is partially filled with a reagent 470 (FIG. 22) which is configured for isolating mononuclear cells, the reagent 470 having a volume Vr. In some embodiments, the reagent 470 comprises Ficoll-Paque® Centrifugation Media, GE Healthcare Bio-Sciences AB, Uppsala, Sweden, or equivalent media. In some embodiments, the reagent 470 comprises Ficoll-Paque® PLUS Centrifugation Media. In some embodiments, the reagent 470 comprises Ficoll-Paque® PREMIUM Centrifugation Media. In some embodiments, the reagent 470 has a density of between about 1.0 grams/ml and about 1.3 grams/ml. In some embodiments, the reagent 470 has a density of between about 1.07 grams/ml and about 1.09 grams/ml. In some embodiments, the reagent 470 has a density of between about 1.073 grams/ml and about 1.084 grams/ml. The volume Vr of the reagent 470 represents a first layer 472 (e.g., bottom layer) in the container 468. Further, as part of step 404, a second layer 474 comprising a volume Vs of the supernatant 476 produced in step 402 is layered directly over first layer 472 in the container 468, as shown in FIG. 22. The layer 472 of the reagent 470 is layered with a height, or column height CHr, that is at least 67% of the height, or column height CHs, of the layer 474 of the supernatant 476. In some embodiments, the column height CHr of the layer 472 of the reagent 470 is at least 75% of the column height CHs of the layer 474 of the supernatant 476. In some embodiments, the column height CHr of the layer 472 of the reagent 470 is at least 85% of the column height CHs of the layer 474 of the supernatant 476. In some embodiments, the column height CHr of the layer 472 of the reagent 470 is between about 30% and about 135% of the column height CHs of the layer 474 of the supernatant 476. In some embodiments, the column height CHr of the layer 472 of the reagent 470 is between about 50% and about 100% of the column height CHs of the layer 474 of the supernatant 476. In some embodiments, the column height CHr of the layer 472 of the reagent 470 is between about 75% and about 100% of the column height CHs of the layer 474 of the supernatant 476. The second layer 474 should be added over the first layer 472 in a slow or at least gentle or non-disruptive manner (e.g., slow speed pipetting) if a distinct interface between the two layers 472, 474 is initially desired.

[0103] In step 406, a sufficient centrifugal force component 478 is placed on the container 468 (FIG. 23) and its contents (e.g., first layer 472 and second layer 474), with the centrifugal force component 478 directed toward a closed container end 480, to cause layering as shown in FIG. 24 to form. FIG. 23 illustrates the basic mechanics of a centrifuge

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PCT/US2018/034584 which spins the container 468 in a circular path 482 around a center point 484. The container 468 may, for example, be carefully placed within the centrifuge 46 in an appropriate location for spinning. In FIG. 24, a layer 486 comprises granulocytes and erythrocytes, and has migrated below a layer 472a containing the reagent 470, and which remains from the first layer 472 of FIG. 22. Layer 488 substantially comprises mononuclear cells, and layer 490 substantially comprises plasma and platelets. In some embodiments, step 406 may be performed using a centrifuge which places at least about 350 times the acceleration due to gravity on the container 468 and its contents. In some embodiments, step 406 may be performed using a centrifuge which places at least about 400 times the acceleration due to gravity on the container 468 and its contents. In some embodiments, step 406 may be performed using a centrifuge which places at least about 450 times the acceleration due to gravity on the container 468 and its contents. In some embodiments, step 406 may be performed using a centrifuge which places at least about 600 times the acceleration due to gravity on the container 468 and its contents. In some embodiments, step 406 may be performed using a centrifuge which places at least about 800 times the acceleration due to gravity on the container 468 and its contents. In some embodiments, step 406 may be performed using a centrifuge which places at least about 1,400 times the acceleration due to gravity on the container 468 and its contents. In some embodiments, the centrifuge may be run between about 5 minutes and about 30 minutes to achieve the desired result. In one embodiment, step 406 is performed using a centrifuge which places between about 750 times the acceleration due to gravity to about 900 times the acceleration due to gravity on the container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 16°C and 22°C. In another embodiment, step 406 is performed using a centrifuge which places between about 800 times the acceleration due to gravity to about 850 times the acceleration due to gravity on the container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 16°C and 22°C. In another embodiment, step 406 is performed using a centrifuge which places between about 750 times the acceleration due to gravity to about 900 times the acceleration due to gravity on the container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 17°C and 21 °C. In another embodiment, step 406 is performed using a centrifuge which places between about 800 times the acceleration due to gravity to about 850 times the acceleration due to gravity on the

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PCT/US2018/034584 container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 17°C and 21 °C.

[0104] In addition to controlling the amount of centrifugal force on the contents, the temperature on the contents, and/or the amount of time that the centrifuge is operated, a user may also choose to control the ramp up and ramp down (run profile) of the centrifuge; in other words, the time required to reach the chosen centrifugal force from a stopped condition, and the time required to reach a stopped condition from the nominally chosen centrifugal force. In a traditional centrifuge, this corresponds to the increase of the rotational speed (e.g., RPM) from a stopped condition until a desired constant operation rate is reached that provides the desired relative centrifugal force, and the decrease of the rotational speed (from a desired constant operation rate) to a stopped condition (braking). This may also be referred to as a run profile. The start-up acceleration portion of the profile may be described as an acceleration curve, and the deceleration portion of the profile may be described as a braking curve. In certain centrifuges, for example, the acceleration may be controllable by a continuous adjustment or may have particular set points (e.g., 1, 2, 3, 4, 5, etc.). Additionally, deceleration (e.g., braking) may by controllable by continuous adjustment or may have particular set points (e.g., 1, 2, 3, 4, 5, etc.). The control of one or both of these two elements can be used to further optimize the effectiveness of the centrifugation. The time to ramp up to a desired centrifugal force from substantially zero centrifugal force may be between about 30 seconds and about 15 minutes, or between about one minute and about five minutes, or between about 10 seconds and 90 seconds. The time to ramp down from a desired centrifugal force to substantially zero centrifugal force may be between about 30 seconds and about 20 minutes, or between about one minute and about 10 minutes.

[0105] In step 408, mononuclear cells from the mononuclear cell layer 488 are removed from the container 468 and are combined with lactated Ringer’s solution, to create a mononuclear cell solution. Prior to the removal of the mononuclear cells from the container 468, the layer 490 may be first removed (FIG. 25), thus making it easier to remove the layer 488. Care can be taken so that the layer 488 is not disturbed when removing the layer 490, for example, by use of a sterile pipette. In step 408, the mononuclear cells from the mononuclear cell layer 488 are actively (though gently) mixed with the lactated Ringer’s solution. The mononuclear cells may also be washed one, two, or more times with lactated Ringer’s solution. In some embodiments, the mononuclear cell solution substantially comprises nonexpanded cell populations. In some embodiments, the mononuclear cell solution comprises non-pooled cell populations. It should be noted that the layer 490 is substantially removed

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PCT/US2018/034584 prior to removing the mononuclear cells from the layer 488 to minimize any unnecessary contamination by platelets or plasma proteins. It should also be noted that the layer 472a is left substantially undisturbed when removing the layer 488, thus minimizing any unnecessary contamination by the granulocytes in layer 486.

[0106] In step 410, a volume Vm of either a portion of the mononuclear cell solution or substantially all of the mononuclear cell solution is used in order to estimate the number Nl of live cells in the volume Vm. In some embodiments, the estimating is done by performing a CD34+ count. In some embodiments, the estimating is done by performing a Total Nucleated Cell (TNC) count. In some embodiments, the estimating is done at least in part with an automated cell counter. Alternative methods may be used for performing the cell count, including: a hemtocytometer following trypan blue staining, an ATP test, Calcein AM, a clonogenic assay, an ethidium homodimer assay, Evans blue, fluorescein diacetate hydrolysis/propidium iodide staining (FDA/PI staining), flow cytometry, formazan-basedassays (MTT/XTT), green fluorescent protein, lactate dehydrogenase (LDH), methyl violet, propidium iodide DNA stain (to differentiate necrotic, apoptotic, and normal cells), resazurin, trypan blue which only crosses cell membranes of dead cells, or TUNEL assay. At the end of step 410 substantially all of the lactated Ringer’s solution may be removed from the volume Vm.

[0107] In some embodiments, Low Molecular Weight Dextran in Dextrose solution is added to the volume Vm of the at least a portion of the mononuclear cell solution. In some embodiments, the amount of Low Molecular Weight Dextran in Dextrose solution is based at least partially upon the estimated number Nl of live cells, a calculated, estimated or determined. In some embodiments, the calculation, estimation or determination of the volume Vdd pf the Low Molecular Weight Dextran in Dextrose solution added may be at least partially based on the assumption that approximately 75% of cells in the at least a portion mononuclear cell solution will be viable following freezing and thawing. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 100,000 live cells and about 75 million live cells per milliliter. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 750,000 live cells and about 30 million live cells per milliliter. In some embodiments, the cells may be stored cryogenically. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 10 million live cells

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PCT/US2018/034584 and about 30 million live cells per milliliter. In some embodiments, the calculation, estimation or determination of the volume Vdd may be at least partially based on an intended live cell concentration of between about 10 million live cells and about 20 million live cells per milliliter. In some embodiments, the cryogenic storage is below about -60°C. In some embodiments, the cryogenic storage is below about -80°C.

[0108] In some embodiments, the pH of the combined composition may be adjusted if needed, in order to produce a pH within a desired range. In some embodiments, the pH is adjusted by adding a sodium bicarbonate, such as sodium bicarbonate powder. In some embodiments, the pH is adjusted by adding a calcium bicarbonate, such as calcium bicarbonate solution. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 8.5. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 7.2. In some embodiments, the pH is adjusted to or maintained at between 4.1 and 6.2. In some embodiments, the pH is adjusted to or maintained at between 5.0 and 7.4. In some embodiments, the pH is adjusted to or maintained at between 5.0 and 7.2. In some embodiments, the pH is adjusted to or maintained at between 5.0 and 6.2.

[0109] In some embodiments, the mononuclear cell solution comprises stem cells. In some embodiments, the stem cells comprise hemapoietic stem cells (HSC). In some embodiments, the stem cells comprise mesenchymal stem cells (MSC).

[0110] In some embodiments, one or more bioactive agents may be added to the composition. The one or more bioactive agents may include cytokines or growth factors, and may be derived from human umbilical cord blood. In some embodiments, the one or more bioactive agents may be obtained or derived from the same sample comprising human umbilical cord blood that is described in step 400. In some embodiments, the one or more bioactive agents may be obtained or derived from human umbilical cord blood that is not the sample comprising human umbilical cord blood that is described in step 400. In some embodiments, the one or more bioactive agents may be obtained or derived from human umbilical cord blood that is from a different donor than the sample comprising human umbilical cord blood that is described in step 400. In some embodiments, the one or more bioactive agents are obtained or derived from commercially available human albumin or commercially available human albumin serum.

[0111] In some embodiments, the one or more bioactive agents may include but are not limited to cytokines or growth factors. The bioactive agents may include any of the following: chemokines, including macrophage inflammatory protein alpha (MIP-la), macrophage inflammatory protein beta (ΜΙΡ-Ιβ), interferon gamma-induced protein 10 (IP

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10), interleukins, including interleukin 1 Beta (IL-Ιβ), interleukin 2 (IL-2), interleukin 4 (IL4), interleukin 5 (IL-5), interleukin 6 (IL-6), interleukin 7 (IL-7), interleukin 8 (IL-8), interleukin 9 (IL-9), interleukin 10 (IL-10), interleukin 12 (IL-12), interleukin 13 (IL-13), interleukin 15 (IL-15), interleukin 17 (IL-17), interleukin 18 (IL-18), interleukin 21 (IL-21), interleukin 22 (IL-22), interleukin 23 (IL-23), interleukin 27 (IL-27), interleukin 31 (IL-31), interferons, including interferon alpha (INPa), a lymphokine, granulocyte macrophage colony stimulating factor (GM-CSF), stem cell factor (KL), tumor necrosis factor alpha (TNF-a), vascular endothelial growth factor (VEGF), nerve growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor 2 (FGF-2), epidermal growth factor (EGF), keratinocyte growth factor, transforming growth factor (TGF), insulin-like growth factor, des(l-3)-IGF-I (brain IGF-I), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), eotaxin, CXC ligand 1 (GROa), interleukin 1 receptor-alpha (IL-IRA), interleukin 1 alpha (IL-1 a), leukemia inhibitory factor (LIF), placental growth factor (PIGF, PLGF), chemokine ligand 5 (RANTES), stem cell factor (SCF), stromal cell derived factor (SDFla), and tumor necrosis factor (TNF β).

[0112] In some embodiments, dimethyl sulfoxide (DMSO) is not added into the composition in any of the steps, thus leaving the composition substantially DMSO-free, and to avoid any potential harmful effects that DMSO may have. The combined composition may then be cryogenically stored, for example, in cryogenic vials in an ultra-low freezer. The cryogenic storage may be controlled at a temperature below -60°C, or below -80°C.

[0113] In some embodiments, the amount of Low Molecular Weight Dextrose solution having a low pH is based at least partially upon the estimated number Nl of live cells, a calculated, estimated or determined, Furthermore, the sedimentation processing and centrifugation techniques described herein to optimize concentrations of one or more particular cytokines within the final composition. Centrifugation processes can be used to enrich the mononuclear cell layer, but can also be used to obtain the extracellular cytokines and growth factors associated with the mononuclear cell layer. The initial nature and concentration of the cytokines (e.g., growth factors, etc.) present in the final collected layer are typically independent of the viable cell count associated with this layer. Physiological stimuli can release a significant amount of cytokines into circulation that will eventually be present in umbilical cord blood. Physical conditions may be controlled during the production of the compositions described herein to control the concentrations of one or more particular cytokines. External factors may include ambient temperature of collection, ambient temperature of storage, ambient temperature of transport, and ambient temperature of

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PCT/US2018/034584 processing. External factors may include the existence of particular anti-coagulants (EDTA, etc). The total time of each portion of the process may also act to control the amount of cytokines, including the total time duration between collection of cord blood and final processing, or the total time duration of processing. The effects from controlling centrifugation (e.g., controlling applied centrifugal force) can be used to optimize the formation of the mononuclear cell layer. The centrifuge speed can also be adjusted to adjust cytokine composition/configuration or cytokine concentration. The physio-chemical properties of any diluents used or other factors in which cells are suspended can help determine the biological half-life of the cytokines.

[0114] For example, step 406 is performed using a centrifuge which places between about 750 times the acceleration due to gravity to about 900 times the acceleration due to gravity on the container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 16°C and 22°C. In another embodiment, step 406 is performed using a centrifuge which places between about 800 times the acceleration due to gravity to about 850 times the acceleration due to gravity on the container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 16°C and 22°C. In another embodiment, step 406 is performed using a centrifuge which places between about 750 times the acceleration due to gravity to about 900 times the acceleration due to gravity on the container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 17°C and 21 °C. In another embodiment, step 406 is performed using a centrifuge which places between about 800 times the acceleration due to gravity to about 850 times the acceleration due to gravity on the container 468 and its contents for a time period of between 15 minutes and 25 minutes, while maintaining the contents of the container 468 at between 17°C and 21 °C.

[0115] The time to ramp up to a desired centrifugal force from substantially zero centrifugal force may be between about 30 seconds and about 15 minutes, or between about one minute and about five minutes, or between about 10 seconds and 90 seconds. The time to ramp down from a desired centrifugal force to substantially zero centrifugal force may be between about 30 seconds and about 20 minutes, or between about one minute and about 10 minutes.

[0116] Particular steps in aliquoting mononuclear cells can be varied slightly to vary concentrations of multiple different cytokines within the composition, such as controlling the concentration ranges of two different cytokines, three different cytokines, four

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PCT/US2018/034584 different cytokines, five different cytokines, six different cytokines, seven different cytokines, eight different cytokines, nine different cytokines, ten different cytokines, and up to the 45 different cytokines, listed below. Effective concentrations within the composition of particular cytokines are listed in Table 1 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
BDNF	Brain-derived neurotrophic factor	492	915
bNGF	Beta-nerve growth factor	14	26
EGF	Epidermal growth factor	715	1329
Eotaxin	Eotaxin	37	69
FGF-2	Fibroblast growth factor-2	28	53
GM-CSF	Granulocyte-macrophage colony stimulating factor	7	15
Gro-alpha	Growth-regulated alpha protein	2974	5524
HGF	Hepatocyte growth factor	352	656
IFN-alpha	Interferon alpha	253	472
IFNgamma	Interferon gamma	33	62
IL-10	Interleukin-10	67	126
IL-12p70	Interleukin-12, heterodimer p70	9	19
IL-13	Interleukin-13	11	22
IL-15	Interleukin-15	30	57
IL-17A	Interleukin-17A	19	37
IL-18	Interleukin-18	78	146
IL-1 alpha	Interleukin-1 alpha	712	1324
IL-lb eta	Interleukin-1 beta	14	28
IL-IRA	Interleukin-1 receptor-alpha	2215	4114
IL-2	Interleukin-2	16	31
IL-21	Interleukin-21	370	689
IL-22	Interleukin-22	16	32
IL-23	Interleukin-23	201	376
IL-27	Interleukin-27	21	40
IL-31	Interleukin-31	11	22
IL-4	Interleukin-4	17	33
IL-5	Interleukin-5	15	28
IL-6	Interleukin-6	50	95
IL-7	Interleukin-7	26	50
IL-8	Interleukin-8	4504	8366
IL-9	Interleukin-9	131	245
IP-10	Interferon gamma-induced protein-10	469	873
LIF	Leukemia inhibitory factor	41	77
MCP-1	Monocyte chemoattractant protein-1	14,480	26,893

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MIP-1 alpha	Macrophage inflammatory protein-1 alpha	303	565
MIP-lbeta	Macrophage inflammatory protein-1 beta	375	698
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	807	1500
PIGF-1	Placenta growth factor-1	743	1381
RANTES	Chemokine (C-C motif) ligand 5	3277	6087
SCF	SCF complex	23	45
sdf- 1 alpha	Stromal cell-derived factor-1 alpha	1114	2071
TNF-alpha	Tumor necrosis factor alpha	42	80
TNF-beta	Tumor necrosis factor beta	12	25
VEGF-A	Vascular endothelial growth factor-A	481	895
VEGF-D	Vascular endothelial growth factor-D	46	88

Table 1 [0117] In addition to optimization of concentrations of one or more particular cytokines within the final composition, one or more inspection process may also be used to reject compositions produced wherein one or more cytokines within the composition is at an undesirable concentration, such as too high or too low. In some embodiments, one part of the composition is diluted with four parts of mesenchymal stem cell growth media. The dilution is then incubated for at least 24 hours, or in other embodiments, for at least 48 hours, in a humidified chamber. The humidified chamber in some embodiments includes an environment of between about 5% carbon di-oxide and is maintained at a temperature of about 37°C. The dilution (also known as conditioned media) is used in a assay to determine the concentrations of the various cytokines. In some embodiments, a Luminex® MAGPIC® system may be used for performing the assay. The normalized concentrations determined in the assay are than multiplied by a factor of 5, to determine the concentrations of the cytokines in the undilute composition. The concentrations may be expressed in units of picograms per milliliter (picograms/ml). An alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 2 below.

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Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
BDNF	Brain-derived neurotrophic factor	527	880
bNGF	Beta-nerve growth factor	15	25
EGF	Epidermal growth factor	766	1278
Eotaxin	Eotaxin	39	67
FGF-2	Fibroblast growth factor-2	30	51
GM-CSF	Granulocyte-macrophage colony stimulating factor	8	15
Gro-alpha	Growth-regulated alpha protein	3186	5312
HGF	Hepatocyte growth factor	378	631
IFN-alpha	Interferon alpha	271	454
IFNgamma	Interferon gamma	35	60
IL-10	Interleukin-10	72	121
IL-12p70	Interleukin-12, heterodimer p70	10	18
IL-13	Interleukin-13	12	21
IL-15	Interleukin-15	32	55
IL-17A	Interleukin-17A	21	35
IL-18	Interleukin-18	83	140
IL-1 alpha	Interleukin-1 alpha	763	1273
IL-lb eta	Interleukin-1 beta	15	27
IL-IRA	Interleukin-1 receptor-alpha	2373	3956
IL-2	Interleukin-2	17	30
IL-21	Interleukin-21	396	662
IL-22	Interleukin-22	17	30
IL-23	Interleukin-23	216	361
IL-27	Interleukin-27	22	38
IL-31	Interleukin-31	12	21
IL-4	Interleukin-4	18	32
IL-5	Interleukin-5	16	27
IL-6	Interleukin-6	54	91
IL-7	Interleukin-7	28	48
IL-8	Interleukin-8	4826	8044
IL-9	Interleukin-9	141	236
IP-10	Interferon gamma-induced protein-10	503	840
LIF	Leukemia inhibitory factor	44	74
MCP-1	Monocyte chemoattractant protein-1	15,514	25,859
MIP-1 alpha	Macrophage inflammatory protein-1 alpha	325	543
MIP-lbeta	Macrophage inflammatory protein-1 beta	402	671
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	865	1442

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PIGF-1	Placenta growth factor-1	796	1328
RANTES	Chemokine (C-C motif) ligand 5	3511	5853
SCF	SCF complex	25	43
SDF1 alpha	Stromal cell-derived factor-1 alpha	1194	1991
TNF-alpha	Tumor necrosis factor alpha	45	77
TNF-beta	Tumor necrosis factor beta	13	24
VEGF-A	Vascular endothelial growth factor-A	516	861
VEGF-D	Vascular endothelial growth factor-D	50	84

Table 2 [0118] Most or all of these cytokines as quantified in the above ranges act as important signaling molecules that regulate biological functions associated with tissue regeneration, such as cell proliferation, cell migration, chemotaxis, angiogenesis, and remodeling. Many of the cytokines listed have at least some overlapping functions. For example, both PDGF and VEGF can support angiogenesis. Some cytokines can perform more than one function. For example, PDGF can be important in both proliferation and angiogenesis. An alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 3 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
BDNF	Brain-derived neurotrophic factor	562	844
bNGF	Beta-nerve growth factor	16	24
EGF	Epidermal growth factor	817	1227
Eotaxin	Eotaxin	42	64
FGF-2	Fibroblast growth factor-2	32	49
GM-CSF	Granulocyte-macrophage colony stimulating factor	9	14
Gro-alpha	Growth-regulated alpha protein	3399	5099
HGF	Hepatocyte growth factor	403	605
IFN-alpha	Interferon alpha	290	436
IFNgamma	Interferon gamma	38	57
IL-10	Interleukin-10	77	117
IL-12p70	Interleukin-12, heterodimer p70	11	18
IL-13	Interleukin-13	13	20

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IL-15	Interleukin-15	34	53
IL-17A	Interleukin-17A	22	34
IL-18	Interleukin-18	89	135
IL-1 alpha	Interleukin-1 alpha	814	1222
IL-lb eta	Interleukin-1 beta	17	26
IL-IRA	Interleukin-1 receptor-alpha	2531	3798
IL-2	Interleukin-2	18	29
IL-21	Interleukin-21	423	636
IL-22	Interleukin-22	19	29
IL-23	Interleukin-23	230	347
IL-27	Interleukin-27	24	37
IL-31	Interleukin-31	13	20
IL-4	Interleukin-4	19	30
IL-5	Interleukin-5	17	26
IL-6	Interleukin-6	58	88
IL-7	Interleukin-7	30	46
IL-8	Interleukin-8	5147	7722
IL-9	Interleukin-9	150	226
IP-10	Interferon gamma-induced protein-10	537	806
LIF	Leukemia inhibitory factor	47	71
MCP-1	Monocyte chemoattractant protein-1	16,549	24,824
MIP-1 alpha	Macrophage inflammatory protein-1 alpha	347	522
MIP-lbeta	Macrophage inflammatory protein-1 beta	429	644
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	922	1385
PIGF-1	Placenta growth factor-1	849	1275
RANTES	Chemokine (C-C motif) ligand 5	3745	5619
SCF	SCF complex	27	42
SDF1 alpha	Stromal cell-derived factor-1 alpha	1273	1911
TNF-alpha	Tumor necrosis factor alpha	49	74
TNF-beta	Tumor necrosis factor beta	14	23
VEGF-A	Vascular endothelial growth factor-A	550	826
VEGF-D	Vascular endothelial growth factor-D	53	81

Table 3 [0119] An alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 4 below.

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Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
BDNF	Brain-derived neurotrophic factor	597	809
bNGF	Beta-nerve growth factor	17	23
EGF	Epidermal growth factor	868	1176
Eotaxin	Eotaxin	45	61
FGF-2	Fibroblast growth factor-2	34	47
GM-CSF	Granulocyte-macrophage colony stimulating factor	9	13
Gro-alpha	Growth-regulated alpha protein	3611	4887
HGF	Hepatocyte growth factor	428	580
IFN-alpha	Interferon alpha	308	418
IFNgamma	Interferon gamma	40	55
IL-10	Interleukin-10	82	112
IL-12p70	Interleukin-12, heterodimer p70	12	17
IL-13	Interleukin-13	13	19
IL-15	Interleukin-15	36	51
IL-17A	Interleukin-17A	23	33
IL-18	Interleukin-18	95	129
IL-1 alpha	Interleukin-1 alpha	865	1171
IL-lb eta	Interleukin-1 beta	18	25
IL-IRA	Interleukin-1 receptor-alpha	2689	3640
IL-2	Interleukin-2	20	28
IL-21	Interleukin-21	449	609
IL-22	Interleukin-22	20	28
IL-23	Interleukin-23	245	332
IL-27	Interleukin-27	25	35
IL-31	Interleukin-31	13	19
IL-4	Interleukin-4	21	29
IL-5	Interleukin-5	18	25
IL-6	Interleukin-6	61	84
IL-7	Interleukin-7	32	44
IL-8	Interleukin-8	5469	7401
IL-9	Interleukin-9	160	217
IP-10	Interferon gamma-induced protein-10	570	772
LIF	Leukemia inhibitory factor	50	68
MCP-1	Monocyte chemoattractant protein-1	17,583	23,790
MIP-1 alpha	Macrophage inflammatory protein-1 alpha	369	500
MIP-lbeta	Macrophage inflammatory protein-1 beta	455	617
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	980	1327

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PIGF-1	Placenta growth factor-1	902	1222
RANTES	Chemokine (C-C motif) ligand 5	3979	5385
SCF	SCF complex	29	40
SDF1 alpha	Stromal cell-derived factor-1 alpha	1353	1832
TNF-alpha	Tumor necrosis factor alpha	52	71
TNF-beta	Tumor necrosis factor beta	15	22
VEGF-A	Vascular endothelial growth factor-A	585	792
VEGF-D	Vascular endothelial growth factor-D	56	78

Table 4 [0120] An alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 5 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
BDNF	Brain-derived neurotrophic factor	632	774
bNGF	Beta-nerve growth factor	18	22
EGF	Epidermal growth factor	919	1124
Eotaxin	Eotaxin	47	59
FGF-2	Fibroblast growth factor-2	36	45
GM-CSF	Granulocyte-macrophage colony stimulating factor	10	13
Gro-alpha	Growth-regulated alpha protein	3823	4674
HGF	Hepatocyte growth factor	453	555
IFN-alpha	Interferon alpha	326	399
IFNgamma	Interferon gamma	42	53
IL-10	Interleukin-10	87	107
IL-12p70	Interleukin-12, heterodimer p70	12	16
IL-13	Interleukin-13	14	19
IL-15	Interleukin-15	39	48
IL-17A	Interleukin-17A	25	31
IL-18	Interleukin-18	100	124
IL-1 alpha	Interleukin-1 alpha	916	1121
IL-lb eta	Interleukin-1 beta	19	24
IL-IRA	Interleukin-1 receptor-alpha	2848	3481
IL-2	Interleukin-2	21	26
IL-21	Interleukin-21	476	583

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IL-22	Interleukin-22	21	27
IL-23	Interleukin-23	259	318
IL-27	Interleukin-27	27	34
IL-31	Interleukin-31	14	18
IL-4	Interleukin-4	22	28
IL-5	Interleukin-5	19	24
IL-6	Interleukin-6	65	81
IL-7	Interleukin-7	34	42
IL-8	Interleukin-8	5791	7079
IL-9	Interleukin-9	169	208
IP-10	Interferon gamma-induced protein-10	604	739
LIF	Leukemia inhibitory factor	53	66
MCP-1	Monocyte chemoattractant protein-1	18,617	22,756
MIP-1 alpha	Macrophage inflammatory protein-1 alpha	390	478
MIP-lbeta	Macrophage inflammatory protein-1 beta	482	590
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	1038	1269
PIGF-1	Placenta growth factor-1	955	1169
RANTES	Chemokine (C-C motif) ligand 5	4213	5151
SCF	SCF complex	30	38
SDF1 alpha	Stromal cell-derived factor-1 alpha	1433	1752
TNF-alpha	Tumor necrosis factor alpha	55	68
TNF-beta	Tumor necrosis factor beta	16	21
VEGF-A	Vascular endothelial growth factor-A	619	758
VEGF-D	Vascular endothelial growth factor-D	60	74

Table 5 [0121] An alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 6 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
BDNF	Brain-derived neurotrophic factor	668	739
bNGF	Beta-nerve growth factor	19	21
EGF	Epidermal growth factor	970	1073

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Eotaxin	Eotaxin	50	56
FGF-2	Fibroblast growth factor-2	38	43
GM-CSF	Granulocyte-macrophage colony stimulating factor	10	12
Gro-alpha	Growth-regulated alpha protein	4036	4462
HGF	Hepatocyte growth factor	478	530
IFN-alpha	Interferon alpha	344	381
IFNgamma	Interferon gamma	45	50
IL-10	Interleukin-10	91	102
IL-12p70	Interleukin-12, heterodimer p70	13	15
IL-13	Interleukin-13	15	18
IL-15	Interleukin-15	41	46
IL-17A	Interleukin-17A	26	30
IL-18	Interleukin-18	106	118
IL-1 alpha	Interleukin-1 alpha	967	1070
IL-lb eta	Interleukin-1 beta	20	23
IL-IRA	Interleukin-1 receptor-alpha	3006	3323
IL-2	Interleukin-2	22	25
IL-21	Interleukin-21	502	556
IL-22	Interleukin-22	22	26
IL-23	Interleukin-23	274	303
IL-27	Interleukin-27	28	32
IL-31	Interleukin-31	15	18
IL-4	Interleukin-4	23	27
IL-5	Interleukin-5	20	23
IL-6	Interleukin-6	69	77
IL-7	Interleukin-7	36	40
IL-8	Interleukin-8	6113	6757
IL-9	Interleukin-9	178	198
IP-10	Interferon gamma-induced protein-10	637	705
LIF	Leukemia inhibitory factor	56	63
MCP-1	Monocyte chemoattractant protein-1	19,652	21,721
MIP-1 alpha	Macrophage inflammatory protein-1 alpha	412	456
MIP-lbeta	Macrophage inflammatory protein-1 beta	509	564
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	1095	1212
PIGF-1	Placenta growth factor-1	1009	1116
RANTES	Chemokine (C-C motif) ligand 5	4447	4916
SCF	SCF complex	32	36
SDF1 alpha	Stromal cell-derived factor-1 alpha	1512	1672
TNF-alpha	Tumor necrosis factor alpha	58	65
TNF-beta	Tumor necrosis factor beta	17	20

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VEGF-A	Vascular endothelial growth factor-A	653	723
VEGF-D	Vascular endothelial growth factor-D	63	71

Table 6 [0122] Cytokines of particular interest include EGF (Epidermal growth factor), PDGF-BB (Platelet-derived growth factor subunit B-heterodimer), VEGF-A (Vascular endothelial growth factor-A), SCF (SCF Complex), IL-IRA (Interleukin-1 receptor-alpha), GM-CSF (Granulocyte-macrophage colony stimulating factor), IL-4 (Interleukin-4), IL-8 (Interleukin-8), SDF-1 alpha (Stromal cell-derived factor-1-alpha), and RANTES (CCL5, Chemokine (C-C motif) ligand 5). The inventors have found that by controlling the concentrations of some or all of these ten cytokines with tighter control than that used in the other cytokines in the tables above can be effective in producing compositions with improved wound healing qualities. For example, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 7 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	715	1329
IL-IRA	Interleukin-1 receptor-alpha	2215	4114
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	807	1500
SCF	SCF complex	23	45
VEGF-A	Vascular endothelial growth factor-A	481	895

Table 7 [0123] In other embodiments, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 8 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	766	1278

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IL-IRA	Interleukin-1 receptor-alpha	2373	3956
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	865	1442
SCF	SCF complex	25	43
VEGF-A	Vascular endothelial growth factor-A	516	861

Table 8 [0124] In other embodiments, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 9 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	817	1227
IL-IRA	Interleukin-1 receptor-alpha	2531	3798
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	922	1385
SCF	SCF complex	27	42
VEGF-A	Vascular endothelial growth factor-A	550	826

Table 9 [0125] In other embodiments, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 10 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	868	1176
IL-IRA	Interleukin-1 receptor-alpha	2689	3640
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	980	1327
SCF	SCF complex	29	40
VEGF-A	Vascular endothelial growth factor-A	585	792

Table 10

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PCT/US2018/034584 [0126] In other embodiments, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 11 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	919	1124
IL-IRA	Interleukin-1 receptor-alpha	2848	3481
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	1038	1269
SCF	SCF complex	30	38
VEGF-A	Vascular endothelial growth factor-A	619	758

Table 11 [0127] In other embodiments, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 12 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	970	1073
IL-IRA	Interleukin-1 receptor-alpha	3006	3323
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	1095	1212
SCF	SCF complex	32	36
VEGF-A	Vascular endothelial growth factor-A	653	723

Table 12 [0128] In other embodiments, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 13 below.

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Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	715	1329
GM-CSF	Granulocyte-macrophage colony stimulating factor	7	15
IL-IRA	Interleukin-1 receptor-alpha	2215	4114
IL-4	Interleukin-4	17	33
IL-8	Interleukin-8	4504	8366
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	807	1500
RANTES	Chemokine (C-C motif) ligand 5	3277	6087
SCF	SCF complex	23	45
SDF1 alpha	Stromal cell-derived factor-1 alpha	1114	2071
VEGF-A	Vascular endothelial growth factor-A	481	895

Table 13 [0129] In other embodiments, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 14 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	766	1278
GM-CSF	Granulocyte-macrophage colony stimulating factor	8	15
IL-IRA	Interleukin-1 receptor-alpha	2373	3956
IL-4	Interleukin-4	18	32
IL-8	Interleukin-8	4826	8044
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	865	1442
RANTES	Chemokine (C-C motif) ligand 5	3511	5853
SCF	SCF complex	25	43
SDF1 alpha	Stromal cell-derived factor-1 alpha	1194	1991
VEGF-A	Vascular endothelial growth factor-A	516	861

Table 14

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PCT/US2018/034584 [0130] In other embodiments, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 15 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	817	1227
GM-CSF	Granulocyte-macrophage colony stimulating factor	9	14
IL-IRA	Interleukin-1 receptor-alpha	2531	3798
IL-4	Interleukin-4	19	30
IL-8	Interleukin-8	5147	7722
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	922	1385
RANTES	Chemokine (C-C motif) ligand 5	3745	5619
SCF	SCF complex	27	42
SDF1 alpha	Stromal cell-derived factor-1 alpha	1273	1911
VEGF-A	Vascular endothelial growth factor-A	550	826

Table 15 [0131] In other embodiments, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 16 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	868	1176
GM-CSF	Granulocyte-macrophage colony stimulating factor	9	13
IL-IRA	Interleukin-1 receptor-alpha	2689	3640
IL-4	Interleukin-4	21	29
IL-8	Interleukin-8	5469	7401
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	980	1327
RANTES	Chemokine (C-C motif) ligand 5	3979	5385
SCF	SCF complex	29	40
SDF-	Stromal cell-derived factor-1	1353	1832

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1 alpha	alpha
VEGF-A	Vascular endothelial growth factor-A	585	792

Table 16 [0132] In other embodiments, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 17 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	919	1124
GM-CSF	Granulocyte-macrophage colony stimulating factor	10	13
IL-IRA	Interleukin-1 receptor-alpha	2848	3481
IL-4	Interleukin-4	22	28
IL-8	Interleukin-8	5791	7079
PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	1038	1269
RANTES	Chemokine (C-C motif) ligand 5	4213	5151
SCF	SCF complex	30	38
SDF1 alpha	Stromal cell-derived factor-1 alpha	1433	1752
VEGF-A	Vascular endothelial growth factor-A	619	758

Table 17 [0133] In other embodiments, an alternative composition may be produced by varying particular steps in aliquoting mononuclear cells and/or by use of the inspection and acceptance or rejection process, to produce effective concentrations within the composition of particular cytokines, as listed in Table 18 below.

Cytokine Symbol	Cytokine	Minimum Concentration (picograms/ml)	Maximum Concentration (picograms/ml)
EGF	Epidermal growth factor	970	1073
GM-CSF	Granulocyte-macrophage colony stimulating factor	10	12
IL-IRA	Interleukin-1 receptor-alpha	3006	3323
IL-4	Interleukin-4	23	27
IL-8	Interleukin-8	6113	6757

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PDGF-BB	Platelet-derived growth factor subunit B-heterodimer	1095	1212
RANTES	Chemokine (C-C motif) ligand 5	4447	4916
SCF	SCF complex	32	36
SDF1 alpha	Stromal cell-derived factor-1 alpha	1512	1672
VEGF-A	Vascular endothelial growth factor-A	653	723

Table 18 [0134] FIGS. 26A-28B illustrate compositions comprising embodiments described herein in use in medical treatment methods. FIGS. 26A-26C illustrate a procedure for spinal fusion using a composition of an embodiment described herein. A spine 52 of a patient includes two adjacent vertebrae 54, 56 which are to be fused. It may be desired to fuse two or more vertebrae to treat a number of different ailments, including, but not limited to: scoliosis, spondylolisthesis, hyperkyphosis, hyperlordosis (or hypolordosis/flat-back syndrome, hypokyphosis), degenerative disk disease (DDD), spinal stenosis, trauma, including vertebral fracture, tumor, revision surgery, infection, pseudoarthrosis, herniated disk, spondylolysis, mechanical instability, facet syndrome, chronic back pain, and radiating leg pain. In FIG. 26A, a patient’s vertebrae 54, 56 are at least partially exposed and an intervertebral disk 58 is removed. In some cases, the disk 58 may already have been removed or may be missing or damaged, and thus not require removal. In some cases, at least a portion of the disk may be purposely left in place. Though in some cases it is optional, in FIG. 26B, a load-bearing device 60 is placed between the first vertebrae 54 and the second vertebrae 56. The loadbearing device 60 may include a fusion cage or interbody device. In FIG. 26C, a biological composition 62, such as any embodiment of those described herein, is placed between the vertebrae 54, 56 and, when applicable, around or adjacent to the load-bearing device 60, including within any cavities in the interbody device, should there be any. The biological composition 62, may also be placed near or at posterior-lateral gutters. Procedures may include posterior lumbar interbody fusion (PLIF), transforaminal lumbar interbody fusion (TLIF), anterior lumbar interbody fusion (ALIF), extreme lateral interbody fusion (XLIF), direct lateral interbody fusion (DLIF), or axial lumbar interbody fusion (AliaLIF). An appropriate amount of the biological composition 62 is placed so that it is effective in aiding the fusion of the two vertebrae 54, 56. In some cases the patient may be skeletally mature. In other cases, the patient may be skeletally immature.

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PCT/US2018/034584 [0135] In some cases, an effective amount of the biological composition 62 to promote, aid, or accelerate fusion of the first vertebra 54 to the second vertebra 56 may comprise between about 1% and 50% of a volume to be filled, or about 3% to 25%, or about 5% to 20%, or about 10%. The remainder of the volume to be filled may be filled with bone graft, or other materials, or left at least partially unfilled. For example, in a disc space of about 10 ml, about 0.5 ml to 2.0 ml of the biological composition 62 may be inserted, or in a disc space of about 5 ml, about 0.25 ml to about 1.0 ml of the biological composition 62 may be inserted. The biological composition 62 may be applied substantially to a particular location within the volume to be filled, or alternatively may be applied in diffuse locations within the volume to be filled. In some embodiments, other materials may be added to the biological composition 62, including synthetic bone substitutes such as beta tricalcium phosphate, or actual pieces of bone, including ground bone.

[0136] FIGS. 27A-27B illustrate a procedure for fusion using a composition of an embodiment described herein. A segment 72 of the skeletal system of a patient includes a first bone portion 74 and a second bone portion 76 which are to be fused. It may be desired to fuse two or more bone portions to treat a number of different ailments, including, but not limited to: trauma, including fracture, tumor, arthritis, ankylosis, deformity, congenital defects, bone length discrepancy, revision surgery, infection, osteonecrosis, osteoporosis, pseudoarthrosis, non-union, delayed-union, and pain. The bone portions 74, 76 may both be within a particular portion of the spine (cervical, thoracic, lumbar, sacral) or may be within two different portions (thoracic to lumbar, lumbar to sacral, etc.). In FIG. 27A, the first bone portion 74 and the second bone portion 76 are at least partially exposed. Surfaces in one or each of the first and second bone portions 74, 76 may then be prepared, for example by cutting (osteotomy) or roughening. In some cases, a load-bearing device 75, such as a plate, rod, nail, screw, hook, or pin may be used to hold the first bone portion 74 and the second bone portion 76 in relation to one another. In FIG. 27B, a biological composition 78, such as any embodiment of those described herein, is placed between the first and second bone portions 74, 76 and, when applicable, around or adjacent to the load-bearing device 75. An appropriate amount of the biological composition 78 is placed so that it is effective in aiding the fusion of first and second bone portions 74, 76. Any of the bones of the skeletal system may be fused while utilizing the biological composition 78. In some cases, the patient may be skeletally mature. In other cases, the patient may be skeletally immature.

[0137] In some cases, an effective amount of the biological composition 78 to promote, aid, or accelerate fusion of the first bone portion 74 to the second bone portion 76

WO 2018/226435

PCT/US2018/034584 may comprise between about 1% and 50% of a volume to be filled, or about 3% to 25%, or about 5% to 20%, or about 10%. The remainder of the volume to be filled may be filled with bone graft, or other materials, or left at least partially unfilled. The biological composition 78 may be applied substantially to a particular location within the volume to be filled, or alternatively may be applied in diffuse locations within the volume to be filled. In some embodiments, other materials may be added to the biological composition 78, including synthetic bone substitutes such as beta tricalcium phosphate, or actual pieces of bone, including ground bone.

[0138] FIGS. 28A-28B illustrate a procedure for healing or growth augmentation of soft tissue 84 in a region 82 of a patient. A biological composition 86, such as any embodiment of those described herein, is placed within, near, or adjacent the soft tissue 84. In alternative procedures, partially or fully hard tissue may also or instead be treated with the biological composition 86. In some cases, the patient to be treated has at least some cancerous cells, and the biological composition 86 is placed within, near, or adjacent the cancerous cells. In some cases, the patient to be treated had at least some cancerous cells removed, and the biological composition 86 is placed within, near, or adjacent the previous location (former site) of the cancerous cells. In cancer patients or post-cancer patients, the cancer may include, but is not limited to: lymphatic cancer, including lymph vessel tumors, soft tissue sarcoma, fat tissue tumors, muscle tissue tumors, peripheral nerve tumors, fibrous tissue tumors, joint tissue tumors, and blood vessel tumors. In some cases, the biological composition may be used for vascular reconstruction (e.g., blood vessels). The patients treated may include adult or pediatric patients. In some cases, an effective amount of the biological composition 86 to promote healing or growth augmentation of the soft tissue 84 may comprise at least about 0.25 ml, or between about 0.25 ml and about 5 ml, or between about 0.25 ml and about 2 ml.

[0139] Other sources besides umbilical cord blood are possible for the compositions described herein, including, but not limited to amniotic fluid, peripheral blood, umbilical cord tissue, bone marrow, adipose tissue, and central nervous system (CNS) fluid.

[0140] In addition to previously mentioned procedures, other procedures may be performed incorporating the compositions and methods of manufacture of the embodiments described herein, including, but not limited to procedures for the treatment of diabetes mellitus and/or its related symptoms, procedures for the treatment of diabetic neuropathy, procedures for the treatment of diabetic ulcers, procedures for the treatment of epidermal, dermal, or sub-dermal diabetic ulcers, procedures for pain management for joints, including

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PCT/US2018/034584 joints having chronic pain, procedures for the treatment of tendonitis, procedures for the treatment of tom cartilage, procedures for the treatment of tendon laxity, such as tendon laxity in joints, including but not limited to, shoulder joints, ankle joints, knee joints, hip joints, and elbow joints, procedures for the treatment of leukemia, procedures for the treatment of lymphoma, procedures for the treatment of myeloma, procedures for the treatment of other cancers, procedures for other epidermal, dermal, or sub-dermal disorders or illnesses, or procedures related to damage to neurons, including Parkinson’s disease and brain tumors. Procedures may be performed incorporating the compositions and methods of manufacture of the embodiments described herein to reduce tumor size. The compositions may in some embodiments, be added in other manners, including epidural application, transthecal application, intravenous (IV) application, or direct site application.

[0141] While embodiments have been shown and described, various modifications may be made without departing from the scope of the inventive concepts disclosed herein.

[0142] The ranges disclosed herein also encompass any and all overlap, subranges, and combinations thereof. Language such as “up to,” “at least,” “greater than,” “less than,” “between,” and the like includes the number recited. Numbers preceded by a term such as “approximately”, “about”, and “substantially” as used herein include the recited numbers (e.g., about 10%=10%), and also represent an amount close to the stated amount that still performs a desired function or achieves a desired result. For example, the terms “approximately”, “about”, and “substantially” may refer to an amount that is within less than 10% of, within less than 5% of, within less than 1% of, within less than 0.1% of, and within less than 0.01% of the stated amount.

Claims

WHAT IS CLAIMED IS:

1. A composition for influencing biological growth comprising:

live cells;

a fluid comprising dextrose;

a protectant; and a first cytokine having a first concentration within the composition, selected from the group consisting of BDNF at between 492 picograms per ml and 915 picograms per ml, bNGF at between 14 picograms per ml and 26 picograms per ml, EGF at between 715 picograms per ml and 1329 picograms per ml, Eotaxin at between 37 picograms per ml and 69 picograms per ml, FGF-2 at between 28 picograms per ml and 53 picograms per ml, GMCSF at between 7 picograms per ml and 15 picograms per ml, Gro-α at between 2974 picograms per ml and 5524 picograms per ml, HGF at between 352 picograms per ml and 656 picograms per ml, IFN-α at between 253 picograms per ml and 472 picograms per ml, IFN-γ at between 33 picograms per ml and 63 picograms per ml, IL-10 at between 67 picograms per ml and 126 picograms per ml, IL-12p70 at between 9 picograms per ml and 19 picograms per ml, IL-13 at between 11 picograms per ml and 22 picograms per ml, IL-15 at between 30 picograms per ml and 57 picograms per ml, IL-17A at between 19 picograms per ml and 37 picograms per ml, IL-18 at between 78 picograms per ml and 146 picograms per ml, IL-Ια at between 712 picograms per ml and 1324 picograms per ml, IL-Ιβ at between 14 picograms per ml and 28 picograms per ml, IL-IRA at between 2215 picograms per ml and 4114 picograms per ml, IL-2 at between 16 picograms per ml and 31 picograms per ml, IL-21 at between 370 picograms per ml and 689 picograms per ml, IL-22 at between 16 picograms per ml and 32 picograms per ml, IL-23 at between 201 picograms per ml and 376 picograms per ml, IL-27 at between 21 picograms per ml and 40 picograms per ml, IL-31 at between 11 picograms per ml and 22 picograms per ml, IL-4 at between 17 picograms per ml and 33 picograms per ml, IL-5 at between 15 picograms per ml and 28 picograms per ml, IL-6 at between 50 picograms per ml and 95 picograms per ml, IL-7 at between 26 picograms per ml and 50 picograms per ml, IL-8 at between 4504 picograms per ml and 8366 picograms per ml, IL-9 at between 131 picograms per ml and 245 picograms per ml, IP-10 at between 469 picograms per ml and 873 picograms per ml, LIF at between 41 picograms per ml and 77 picograms per ml, MCP-1 at between 14,480 picograms per ml and 26,893 picograms per ml, MIP-la at between 303 picograms per ml and 565 picograms per ml, ΜΙΡ-Ιβ at between 375 picograms per ml and 698 picograms per ml, PDGF-BB at between 807 picograms per ml

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PCT/US2018/034584 and 1500 picograms per ml, PIGF-1 at between 743 picograms per ml and 1381 picograms per ml, RANTES at between 3277 picograms per ml and 6087 picograms per ml, SCF at between 23 picograms per ml and 45 picograms per ml, SDF-Ια at between 1114 picograms per ml and 2071 picograms per ml, TNF- a at between 42 picograms per ml and 80 picograms per ml, TNF-β at between 12 picograms per ml and 25 picograms per ml, VEGFA at between 481 picograms per ml and 895 picograms per ml, and VEGF-D at between 46 picograms per ml and 88 picograms per ml.
2. The composition of claim 1, wherein the first cytokine has a concentration within the composition listed therewith in the following group: BDNF at between 527 picograms per ml and 880 picograms per ml, bNGF at between 15 picograms per ml and 25 picograms per ml, EGF at between 766 picograms per ml and 1278 picograms per ml, Eotaxin at between 39 picograms per ml and 67 picograms per ml, FGF-2 at between 30 picograms per ml and 51 picograms per ml, GM-CSF at between 8 picograms per ml and 15 picograms per ml, Gro-α at between 3186 picograms per ml and 5312 picograms per ml, HGF at between 378 picograms per ml and 631 picograms per ml, IFN-α at between 271 picograms per ml and 454 picograms per ml, IFN-γ at between 35 picograms per ml and 60 picograms per ml, IL-10 at between 72 picograms per ml and 121 picograms per ml, IL12p70 at between 10 picograms per ml and 18 picograms per ml, IL-13 at between 12 picograms per ml and 21 picograms per ml, IL-15 at between 32 picograms per ml and 55 picograms per ml, IL-17A at between 21 picograms per ml and 35 picograms per ml, IL-18 at between 83 picograms per ml and 140 picograms per ml, IL-Ια at between 763 picograms per ml and 1273 picograms per ml, IL-ΐβ at between 15 picograms per ml and 27 picograms per ml, IL-IRA at between 2373 picograms per ml and 3956 picograms per ml, IL-2 at between 17 picograms per ml and 30 picograms per ml, IL-21 at between 396 picograms per ml and 662 picograms per ml, IL-22 at between 17 picograms per ml and 30 picograms per ml, IL-23 at between 216 picograms per ml and 361 picograms per ml, IL-27 at between 22 picograms per ml and 38 picograms per ml, IL-31 at between 12 picograms per ml and 21 picograms per ml, IL-4 at between 18 picograms per ml and 32 picograms per ml, IL-5 at between 16 picograms per ml and 27 picograms per ml, IL-6 at between 54 picograms per ml and 91 picograms per ml, IL-7 at between 28 picograms per ml and 48 picograms per ml, IL-8 at between 4826 picograms per ml and 8044 picograms per ml, IL-9 at between 141 picograms per ml and 236 picograms per ml, IP-10 at between 503 picograms per ml and 840 picograms per ml, LIF at between 44 picograms per ml and 74 picograms per ml, MCP-1 at between 15,514 picograms per ml and 25,859 picograms per ml, MIP-la at between 325 picograms

WO 2018/226435

PCT/US2018/034584 per ml and 543 picograms per ml, ΜΙΡ-Ιβ at between 402 picograms per ml and 671 picograms per ml, PDGF-BB at between 865 picograms per ml and 1442 picograms per ml, PIGF-1 at between 796 picograms per ml and 1328 picograms per ml, RANTES at between 3511 picograms per ml and 5853 picograms per ml, SCF at between 25 picograms per ml and 43 picograms per ml, SDF-Ια at between 1194 picograms per ml and 1991 picograms per ml, TNF- a at between 45 picograms per ml and 77 picograms per ml, TNF-β at between 13 picograms per ml and 24 picograms per ml, VEGF-A at between 516 picograms per ml and 861 picograms per ml, and VEGF-D at between 50 picograms per ml and 84 picograms per ml.
3. The composition of claim 1, wherein the first cytokine has a concentration within the composition listed therewith in the following group: BDNF at between 562 picograms per ml and 844 picograms per ml, bNGF at between 16 picograms per ml and 24 picograms per ml, EGF at between 817 picograms per ml and 1227 picograms per ml, Eotaxin at between 42 picograms per ml and 64 picograms per ml, FGF-2 at between 32 picograms per ml and 49 picograms per ml, GM-CSF at between 9 picograms per ml and 14 picograms per ml, Gro-α at between 3399 picograms per ml and 5099 picograms per ml, HGF at between 403 picograms per ml and 605 picograms per ml, IFN-α at between 290 picograms per ml and 436 picograms per ml, IFN-γ at between 38 picograms per ml and 57 picograms per ml, IL-10 at between 77 picograms per ml and 117 picograms per ml, IL12p70 at between 11 picograms per ml and 18 picograms per ml, IL-13 at between 13 picograms per ml and 20 picograms per ml, IL-15 at between 34 picograms per ml and 53 picograms per ml, IL-17A at between 22 picograms per ml and 34 picograms per ml, IL-18 at between 89 picograms per ml and 135 picograms per ml, IL-la at between 814 picograms per ml and 1222 picograms per ml, IL-Ιβ at between 17 picograms per ml and 26 picograms per ml, IL-IRA at between 2531 picograms per ml and 3798 picograms per ml, IL-2 at between 18 picograms per ml and 29 picograms per ml, IL-21 at between 423 picograms per ml and 636 picograms per ml, IL-22 at between 19 picograms per ml and 29 picograms per ml, IL-23 at between 230 picograms per ml and 347 picograms per ml, IL-27 at between 24 picograms per ml and 37 picograms per ml, IL-31 at between 13 picograms per ml and 20 picograms per ml, IL-4 at between 19 picograms per ml and 30 picograms per ml, IL-5 at between 17 picograms per ml and 26 picograms per ml, IL-6 at between 58 picograms per ml and 88 picograms per ml, IL-7 at between 30 picograms per ml and 46 picograms per ml, IL-8 at between 5147 picograms per ml and 7722 picograms per ml, IL-9 at between 150 picograms per ml and 226 picograms per ml, IP-10 at between 537 picograms per ml and 806 picograms

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PCT/US2018/034584 per ml, LIF at between 47 picograms per ml and 71 picograms per ml, MCP-1 at between 16,549 picograms per ml and 24,824 picograms per ml, MIP-la at between 347 picograms per ml and 522 picograms per ml, ΜΙΡ-Ιβ at between 429 picograms per ml and 644 picograms per ml, PDGF-BB at between 922 picograms per ml and 1385 picograms per ml, PIGF-1 at between 849 picograms per ml and 1275 picograms per ml, RANTES at between 3745 picograms per ml and 5619 picograms per ml, SCF at between 27 picograms per ml and 42 picograms per ml, SDF-Ια at between 1273 picograms per ml and 1911 picograms per ml, TNF- a at between 49 picograms per ml and 74 picograms per ml, TNF-β at between 14 picograms per ml and 23 picograms per ml, VEGF-A at between 550 picograms per ml and 826 picograms per ml, and VEGF-D at between 53 picograms per ml and 81 picograms per ml.
4. The composition of claim 1, wherein the first cytokine has a concentration within the composition listed therewith in the following group: BDNF at between 597 picograms per ml and 809 picograms per ml, bNGF at between 17 picograms per ml and 23 picograms per ml, EGF at between 868 picograms per ml and 1176 picograms per ml, Eotaxin at between 45 picograms per ml and 61 picograms per ml, FGF-2 at between 34 picograms per ml and 47 picograms per ml, GM-CSF at between 9 picograms per ml and 13 picograms per ml, Gro-α at between 3611 picograms per ml and 4887 picograms per ml, HGF at between 428 picograms per ml and 580 picograms per ml, IFN-α at between 308 picograms per ml and 418 picograms per ml, IFN-γ at between 40 picograms per ml and 55 picograms per ml, IL-10 at between 82 picograms per ml and 112 picograms per ml, IL12p70 at between 12 picograms per ml and 17 picograms per ml, IL-13 at between 13 picograms per ml and 19 picograms per ml, IL-15 at between 36 picograms per ml and 51 picograms per ml, IL-17A at between 23 picograms per ml and 33 picograms per ml, IL-18 at between 95 picograms per ml and 129 picograms per ml, IL-la at between 865 picograms per ml and 1171 picograms per ml, IL-Ιβ at between 18 picograms per ml and 25 picograms per ml, IL-IRA at between 2689 picograms per ml and 3640 picograms per ml, IL-2 at between 20 picograms per ml and 28 picograms per ml, IL-21 at between 449 picograms per ml and 609 picograms per ml, IL-22 at between 20 picograms per ml and 28 picograms per ml, IL-23 at between 245 picograms per ml and 332 picograms per ml, IL-27 at between 25 picograms per ml and 35 picograms per ml, IL-31 at between 13 picograms per ml and 19 picograms per ml, IL-4 at between 21 picograms per ml and 29 picograms per ml, IL-5 at between 18 picograms per ml and 25 picograms per ml, IL-6 at between 61 picograms per ml and 84 picograms per ml, IL-7 at between 32 picograms per ml and 44 picograms per ml, IL-8 at

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PCT/US2018/034584 between 5469 picograms per ml and 7401 picograms per ml, IL-9 at between 160 picograms per ml and 217 picograms per ml, IP-10 at between 570 picograms per ml and 772 picograms per ml, LIF at between 50 picograms per ml and 68 picograms per ml, MCP-1 at between 17,583 picograms per ml and 23,790 picograms per ml, MIP-la at between 369 picograms per ml and 500 picograms per ml, ΜΙΡ-Ιβ at between 455 picograms per ml and 617 picograms per ml, PDGF-BB at between 980 picograms per ml and 1327 picograms per ml, PIGF-1 at between 902 picograms per ml and 1222 picograms per ml, RANTES at between 3979 picograms per ml and 5385 picograms per ml, SCF at between 29 picograms per ml and 40 picograms per ml, SDF-Ια at between 1353 picograms per ml and 1832 picograms per ml, TNF- a at between 52 picograms per ml and 71 picograms per ml, TNF-β at between 15 picograms per ml and 22 picograms per ml, VEGF-A at between 585 picograms per ml and 792 picograms per ml, and VEGF-D at between 56 picograms per ml and 78 picograms per ml.
5. The composition of claim 1, wherein the first cytokine has a concentration within the composition listed therewith in the following group: BDNF at between 632 picograms per ml and 774 picograms per ml, bNGF at between 18 picograms per ml and 22 picograms per ml, EGF at between 919 picograms per ml and 1124 picograms per ml, Eotaxin at between 47 picograms per ml and 59 picograms per ml, FGF-2 at between 36 picograms per ml and 45 picograms per ml, GM-CSF at between 10 picograms per ml and 13 picograms per ml, Gro-α at between 3823 picograms per ml and 4674 picograms per ml, HGF at between 453 picograms per ml and 555 picograms per ml, IFN-α at between 326 picograms per ml and 399 picograms per ml, IFN-γ at between 42 picograms per ml and 53 picograms per ml, IL-10 at between 87 picograms per ml and 107 picograms per ml, IL12p70 at between 12 picograms per ml and 16 picograms per ml, IL-13 at between 14 picograms per ml and 19 picograms per ml, IL-15 at between 39 picograms per ml and 48 picograms per ml, IL-17A at between 25 picograms per ml and 31 picograms per ml, IL-18 at between 100 picograms per ml and 124 picograms per ml, IL-la at between 916 picograms per ml and 1121 picograms per ml, IL-Ιβ at between 19 picograms per ml and 24 picograms per ml, IL-IRA at between 2848 picograms per ml and 3481 picograms per ml, IL-2 at between 21 picograms per ml and 26 picograms per ml, IL-21 at between 476 picograms per ml and 583 picograms per ml, IL-22 at between 21 picograms per ml and 27 picograms per ml, IL-23 at between 259 picograms per ml and 318 picograms per ml, IL-27 at between 27 picograms per ml and 34 picograms per ml, IL-31 at between 14 picograms per ml and 18 picograms per ml, IL-4 at between 22 picograms per ml and 28 picograms per ml, IL-5 at

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PCT/US2018/034584 between 19 picograms per ml and 24 picograms per ml, IL-6 at between 65 picograms per ml and 81 picograms per ml, IL-7 at between 34 picograms per ml and 42 picograms per ml, IL8 at between 5791 picograms per ml and 7079 picograms per ml, IL-9 at between 169 picograms per ml and 208 picograms per ml, IP-10 at between 604 picograms per ml and 739 picograms per ml, LIF at between 53 picograms per ml and 66 picograms per ml, MCP-1 at between 18,617 picograms per ml and 22,756 picograms per ml, MIP-la at between 390 picograms per ml and 478 picograms per ml, ΜΠΜβ at between 482 picograms per ml and 590 picograms per ml, PDGF-BB at between 1038 picograms per ml and 1269 picograms per ml, PIGF-1 at between 955 picograms per ml and 1169 picograms per ml, RANTES at between 4213 picograms per ml and 5151 picograms per ml, SCF at between 30 picograms per ml and 38 picograms per ml, SDF-Ια at between 1433 picograms per ml and 1752 picograms per ml, TNF- a at between 55 picograms per ml and 68 picograms per ml, TNF-β at between 16 picograms per ml and 21 picograms per ml, VEGF-A at between 619 picograms per ml and 758 picograms per ml, and VEGF-D at between 60 picograms per ml and 74 picograms per ml.
6. The composition of claim 1, wherein the first cytokine has a concentration within the composition listed therewith in the following group: BDNF at between 668 picograms per ml and 739 picograms per ml, bNGF at between 19 picograms per ml and 21 picograms per ml, EGF at between 970 picograms per ml and 1073 picograms per ml, Eotaxin at between 50 picograms per ml and 56 picograms per ml, FGF-2 at between 38 picograms per ml and 43 picograms per ml, GM-CSF at between 10 picograms per ml and 12 picograms per ml, Gro-α at between 4036 picograms per ml and 4462 picograms per ml, HGF at between 478 picograms per ml and 530 picograms per ml, IFN-α at between 344 picograms per ml and 381 picograms per ml, IFN-γ at between 45 picograms per ml and 50 picograms per ml, IL-10 at between 91 picograms per ml and 102 picograms per ml, IL12p70 at between 13 picograms per ml and 15 picograms per ml, IL-13 at between 15 picograms per ml and 18 picograms per ml, IL-15 at between 41 picograms per ml and 46 picograms per ml, IL-17A at between 26 picograms per ml and 30 picograms per ml, IL-18 at between 106 picograms per ml and 118 picograms per ml, IL-la at between 967 picograms per ml and 1070 picograms per ml, IL-Ιβ at between 20 picograms per ml and 23 picograms per ml, IL-IRA at between 3006 picograms per ml and 3323 picograms per ml, IL-2 at between 22 picograms per ml and 25 picograms per ml, IL-21 at between 502 picograms per ml and 556 picograms per ml, IL-22 at between 22 picograms per ml and 26 picograms per ml, IL-23 at between 274 picograms per ml and 303 picograms per ml, IL-27 at between 28

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PCT/US2018/034584 picograms per ml and 32 picograms per ml, IL-31 at between 15 picograms per ml and 18 picograms per ml, IL-4 at between 23 picograms per ml and 27 picograms per ml, IL-5 at between 20 picograms per ml and 23 picograms per ml, IL-6 at between 69 picograms per ml and 77 picograms per ml, IL-7 at between 36 picograms per ml and 40 picograms per ml, IL8 at between 6113 picograms per ml and 6757 picograms per ml, IL-9 at between 178 picograms per ml and 198 picograms per ml, IP-10 at between 637 picograms per ml and 705 picograms per ml, LIF at between 56 picograms per ml and 63 picograms per ml, MCP-1 at between 19,652 picograms per ml and 21,721 picograms per ml, MIP-la at between 412 picograms per ml and 456 picograms per ml, ΜΙΡ-Ιβ at between 509 picograms per ml and 564 picograms per ml, PDGF-BB at between 1095 picograms per ml and 1212 picograms per ml, PIGF-1 at between 1009 picograms per ml and 1116 picograms per ml, RANTES at between 4447 picograms per ml and 4916 picograms per ml, SCF at between 32 picograms per ml and 36 picograms per ml, SDF-Ια at between 1512 picograms per ml and 1672 picograms per ml, TNF- a at between 58 picograms per ml and 65 picograms per ml, TNF-β at between 17 picograms per ml and 20 picograms per ml, VEGF-A at between 653 picograms per ml and 723 picograms per ml, and VEGF-D at between 63 picograms per ml and 71 picograms per ml.
7. The composition of claim 1, further comprising a second cytokine different from the first cytokine and having a second concentration within the composition, selected from the group consisting of BDNF at between 492 picograms per ml and 915 picograms per ml, bNGF at between 14 picograms per ml and 26 picograms per ml, EGF at between 715 picograms per ml and 1329 picograms per ml, Eotaxin at between 37 picograms per ml and 69 picograms per ml, FGF-2 at between 28 picograms per ml and 53 picograms per ml, GMCSF at between 7 picograms per ml and 15 picograms per ml, Gro-α at between 2974 picograms per ml and 5524 picograms per ml, HGF at between 352 picograms per ml and 656 picograms per ml, IFN-α at between 253 picograms per ml and 472 picograms per ml, IFN-γ at between 33 picograms per ml and 63 picograms per ml, IL-10 at between 67 picograms per ml and 126 picograms per ml, IL-12p70 at between 9 picograms per ml and 19 picograms per ml, IL-13 at between 11 picograms per ml and 22 picograms per ml, IL-15 at between 30 picograms per ml and 57 picograms per ml, IL-17A at between 19 picograms per ml and 37 picograms per ml, IL-18 at between 78 picograms per ml and 146 picograms per ml, IL-Ια at between 712 picograms per ml and 1324 picograms per ml, IL-Ιβ at between 14 picograms per ml and 28 picograms per ml, IL-IRA at between 2215 picograms per ml and 4114 picograms per ml, IL-2 at between 16 picograms per ml and 31 picograms per ml, IL-21

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PCT/US2018/034584 at between 370 picograms per ml and 689 picograms per ml, IL-22 at between 16 picograms per ml and 32 picograms per ml, IL-23 at between 201 picograms per ml and 376 picograms per ml, IL-27 at between 21 picograms per ml and 40 picograms per ml, IL-31 at between 11 picograms per ml and 22 picograms per ml, IL-4 at between 17 picograms per ml and 33 picograms per ml, IL-5 at between 15 picograms per ml and 28 picograms per ml, IL-6 at between 50 picograms per ml and 95 picograms per ml, IL-7 at between 26 picograms per ml and 50 picograms per ml, IL-8 at between 4504 picograms per ml and 8366 picograms per ml, IL-9 at between 131 picograms per ml and 245 picograms per ml, IP-10 at between 469 picograms per ml and 873 picograms per ml, LIF at between 41 picograms per ml and 77 picograms per ml, MCP-1 at between 14,480 picograms per ml and 26,893 picograms per ml, MIP-la at between 303 picograms per ml and 565 picograms per ml, ΜΙΡ-Ιβ at between 375 picograms per ml and 698 picograms per ml, PDGF-BB at between 807 picograms per ml and 1500 picograms per ml, PIGF-1 at between 743 picograms per ml and 1381 picograms per ml, RANTES at between 3277 picograms per ml and 6087 picograms per ml, SCF at between 23 picograms per ml and 45 picograms per ml, SDF-Ια at between 1114 picograms per ml and 2071 picograms per ml, TNF- a at between 42 picograms per ml and 80 picograms per ml, TNF-β at between 12 picograms per ml and 25 picograms per ml, VEGFA at between 481 picograms per ml and 895 picograms per ml, and VEGF-D at between 46 picograms per ml and 88 picograms per ml.
8. The composition of claim 7, further comprising a third cytokine different from the first cytokine and the second cytokine and having a third concentration within the composition, selected from the group consisting of BDNF at between 492 picograms per ml and 915 picograms per ml, bNGF at between 14 picograms per ml and 26 picograms per ml, EGF at between 715 picograms per ml and 1329 picograms per ml, Eotaxin at between 37 picograms per ml and 69 picograms per ml, FGF-2 at between 28 picograms per ml and 53 picograms per ml, GM-CSF at between 7 picograms per ml and 15 picograms per ml, Gro-a at between 2974 picograms per ml and 5524 picograms per ml, HGF at between 352 picograms per ml and 656 picograms per ml, IFN-α at between 253 picograms per ml and 472 picograms per ml, IFN-γ at between 33 picograms per ml and 63 picograms per ml, IL10 at between 67 picograms per ml and 126 picograms per ml, IL-12p70 at between 9 picograms per ml and 19 picograms per ml, IL-13 at between 11 picograms per ml and 22 picograms per ml, IL-15 at between 30 picograms per ml and 57 picograms per ml, IL-17A at between 19 picograms per ml and 37 picograms per ml, IL-18 at between 78 picograms per ml and 146 picograms per ml, IL-Ια at between 712 picograms per ml and 1324 picograms

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PCT/US2018/034584 per ml, IL-Ιβ at between 14 picograms per ml and 28 picograms per ml, IL-IRA at between 2215 picograms per ml and 4114 picograms per ml, IL-2 at between 16 picograms per ml and 31 picograms per ml, IL-21 at between 370 picograms per ml and 689 picograms per ml, IL22 at between 16 picograms per ml and 32 picograms per ml, IL-23 at between 201 picograms per ml and 376 picograms per ml, IL-27 at between 21 picograms per ml and 40 picograms per ml, IL-31 at between 11 picograms per ml and 22 picograms per ml, IL-4 at between 17 picograms per ml and 33 picograms per ml, IL-5 at between 15 picograms per ml and 28 picograms per ml, IL-6 at between 50 picograms per ml and 95 picograms per ml, IL7 at between 26 picograms per ml and 50 picograms per ml, IL-8 at between 4504 picograms per ml and 8366 picograms per ml, IL-9 at between 131 picograms per ml and 245 picograms per ml, IP-10 at between 469 picograms per ml and 873 picograms per ml, LIF at between 41 picograms per ml and 77 picograms per ml, MCP-1 at between 14,480 picograms per ml and 26,893 picograms per ml, MIP-la at between 303 picograms per ml and 565 picograms per ml, ΜΙΡ-Ιβ at between 375 picograms per ml and 698 picograms per ml, PDGF-BB at between 807 picograms per ml and 1500 picograms per ml, PIGF-1 at between 743 picograms per ml and 1381 picograms per ml, RANTES at between 3277 picograms per ml and 6087 picograms per ml, SCF at between 23 picograms per ml and 45 picograms per ml, SDF-Ια at between 1114 picograms per ml and 2071 picograms per ml, TNF- a at between 42 picograms per ml and 80 picograms per ml, TNF-β at between 12 picograms per ml and 25 picograms per ml, VEGF-A at between 481 picograms per ml and 895 picograms per ml, and VEGF-D at between 46 picograms per ml and 88 picograms per ml.
9. The composition of claim 8, further comprising a fourth cytokine different from the first cytokine, the second cytokine, and the third cytokine and having a fourth concentration within the composition, selected from the group consisting of: BDNF at between 492 picograms per ml and 915 picograms per ml, bNGF at between 14 picograms per ml and 26 picograms per ml, EGF at between 715 picograms per ml and 1329 picograms per ml, Eotaxin at between 37 picograms per ml and 69 picograms per ml, FGF-2 at between 28 picograms per ml and 53 picograms per ml, GM-CSF at between 7 picograms per ml and 15 picograms per ml, Gro-α at between 2974 picograms per ml and 5524 picograms per ml, HGF at between 352 picograms per ml and 656 picograms per ml, IFN-α at between 253 picograms per ml and 472 picograms per ml, IFN-γ at between 33 picograms per ml and 63 picograms per ml, IL-10 at between 67 picograms per ml and 126 picograms per ml, IL12p70 at between 9 picograms per ml and 19 picograms per ml, IL-13 at between 11 picograms per ml and 22 picograms per ml, IL-15 at between 30 picograms per ml and 57

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PCT/US2018/034584 picograms per ml, IL-17A at between 19 picograms per ml and 37 picograms per ml, IL-18 at between 78 picograms per ml and 146 picograms per ml, IL-Ια at between 712 picograms per ml and 1324 picograms per ml, IL-Ιβ at between 14 picograms per ml and 28 picograms per ml, IL-IRA at between 2215 picograms per ml and 4114 picograms per ml, IL-2 at between 16 picograms per ml and 31 picograms per ml, IL-21 at between 370 picograms per ml and 689 picograms per ml, IL-22 at between 16 picograms per ml and 32 picograms per ml, IL-23 at between 201 picograms per ml and 376 picograms per ml, IL-27 at between 21 picograms per ml and 40 picograms per ml, IL-31 at between 11 picograms per ml and 22 picograms per ml, IL-4 at between 17 picograms per ml and 33 picograms per ml, IL-5 at between 15 picograms per ml and 28 picograms per ml, IL-6 at between 50 picograms per ml and 95 picograms per ml, IL-7 at between 26 picograms per ml and 50 picograms per ml, IL-8 at between 4504 picograms per ml and 8366 picograms per ml, IL-9 at between 131 picograms per ml and 245 picograms per ml, IP-10 at between 469 picograms per ml and 873 picograms per ml, LIF at between 41 picograms per ml and 77 picograms per ml, MCP-1 at between 14,480 picograms per ml and 26,893 picograms per ml, MIP-la at between 303 picograms per ml and 565 picograms per ml, ΜΙΡ-Ιβ at between 375 picograms per ml and 698 picograms per ml, PDGF-BB at between 807 picograms per ml and 1500 picograms per ml, PIGF-1 at between 743 picograms per ml and 1381 picograms per ml, RANTES at between 3277 picograms per ml and 6087 picograms per ml, SCF at between 23 picograms per ml and 45 picograms per ml, SDF-Ια at between 1114 picograms per ml and 2071 picograms per ml, TNF- a at between 42 picograms per ml and 80 picograms per ml, TNF-β at between 12 picograms per ml and 25 picograms per ml, VEGF-A at between 481 picograms per ml and 895 picograms per ml, and VEGF-D at between 46 picograms per ml and 88 picograms per ml.
10. A composition for influencing biological growth comprising:

live cells;

a fluid comprising dextrose;

a protectant;

VEGF-A, at a concentration within the composition of between 481 picograms per ml and 895 picograms per ml;

PDGF-BB, at a concentration within the composition of between 807 picograms per ml and 1500 picograms per ml;

EGF, at a concentration within the composition of between 715 picograms per ml and 1329 picograms per ml;

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SCF, at a concentration within the composition of between 23 picograms per ml and 45 picograms per ml; and

IL-IRA, at a concentration within the composition of between 2215 picograms per ml and 4114 picograms per ml.
11. The composition of claim 10, wherein:

the concentration within the composition of VEGF-A is between 516 picograms per ml and 861 picograms per ml;

the concentration within the composition of PDGF-BB is between 865 picograms per ml and 1442 picograms per ml;

the concentration within the composition of EGF is between 766 picograms per ml and 1278 picograms per ml;

the concentration within the composition of SCF is between 25 picograms per ml and 43 picograms per ml; and the concentration within the composition of IL-IRA is between 2373 picograms per ml and 3956 picograms per ml.
12. The composition of claim 10, wherein:

the concentration within the composition of VEGF-A is between 550 picograms per ml and 826 picograms per ml;

the concentration within the composition of PDGF-BB is between 922 picograms per ml and 1385 picograms per ml;

the concentration within the composition of EGF is between 817 picograms per ml and 1227 picograms per ml;

the concentration within the composition of SCF is between 27 picograms per ml and 42 picograms per ml; and the concentration within the composition of IL-IRA is between 2531 picograms per ml and 3798 picograms per ml.
13. The composition of claim 10, wherein:

the concentration within the composition of VEGF-A is between 585 picograms per ml and 792 picograms per ml;

the concentration within the composition of PDGF-BB is between 980 picograms per ml and 1327 picograms per ml;

the concentration within the composition of EGF is between 868 picograms per ml and 1176 picograms per ml;

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PCT/US2018/034584 the concentration within the composition of SCF is between 29 picograms per ml and 40 picograms per ml; and the concentration within the composition of IL-IRA is between 2689 picograms per ml and 3640 picograms per ml.
14. The composition of claim 10, wherein:

the concentration within the composition of VEGF-A is between 619 picograms per ml and 758 picograms per ml;

the concentration within the composition of PDGF-BB is between 1038 picograms per ml and 1269 picograms per ml;

the concentration within the composition of EGF is between 919 picograms per ml and 1124 picograms per ml;

the concentration within the composition of SCF is between 30 picograms per ml and 38 picograms per ml; and the concentration within the composition of IL-IRA is between 2848 picograms per ml and 3481 picograms per ml.
15. The composition of claim 10, wherein:

the concentration within the composition of VEGF-A is between 653 picograms per ml and 723 picograms per ml;

the concentration within the composition of PDGF-BB is between 1095 picograms per ml and 1212 picograms per ml;

the concentration within the composition of EGF is between 970 picograms per ml and 1073 picograms per ml;

the concentration within the composition of SCF is between 32 picograms per ml and 36 picograms per ml; and the concentration within the composition of IL-IRA is between 3006 picograms per ml and 3323 picograms per ml, and wherein the composition further comprises:

GM-CSF having a concentration within the composition of between 10 picograms per ml and 12 picograms per ml;

IL-4 having a concentration within the composition of between 23 picograms per ml and 27 picograms per ml;

IL-8 having a concentration within the composition of between 6113 picograms per ml and 6757 picograms per ml;

SDF-Ια having a concentration within the composition of between 1512 picograms per ml and 1672 picograms per ml; and

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RANTES having a concentration within the composition of between 4447 picograms per ml and 4916 picograms per ml.
16. A composition for influencing biological growth comprising:

live cells;

a fluid comprising dextrose;

a protectant; and wherein the composition includes the following cytokines with their concentration within the composition: BDNF at between 492 picograms per ml and 915 picograms per ml, bNGF at between 14 picograms per ml and 26 picograms per ml, EGF at between 715 picograms per ml and 1329 picograms per ml, Eotaxin at between 37 picograms per ml and 69 picograms per ml, FGF-2 at between 28 picograms per ml and 53 picograms per ml, GMCSF at between 7 picograms per ml and 15 picograms per ml, Gro-α at between 2974 picograms per ml and 5524 picograms per ml, HGF at between 352 picograms per ml and 656 picograms per ml, IFN-α at between 253 picograms per ml and 472 picograms per ml, IFN-γ at between 33 picograms per ml and 63 picograms per ml, IL-10 at between 67 picograms per ml and 126 picograms per ml, IL-12p70 at between 9 picograms per ml and 19 picograms per ml, IL-13 at between 11 picograms per ml and 22 picograms per ml, IL-15 at between 30 picograms per ml and 57 picograms per ml, IL-17A at between 19 picograms per ml and 37 picograms per ml, IL-18 at between 78 picograms per ml and 146 picograms per ml, IL-Ια at between 712 picograms per ml and 1324 picograms per ml, IL-Ιβ at between 14 picograms per ml and 28 picograms per ml, IL-IRA at between 2215 picograms per ml and 4114 picograms per ml, IL-2 at between 16 picograms per ml and 31 picograms per ml, IL-21 at between 370 picograms per ml and 689 picograms per ml, IL-22 at between 16 picograms per ml and 32 picograms per ml, IL-23 at between 201 picograms per ml and 376 picograms per ml, IL-27 at between 21 picograms per ml and 40 picograms per ml, IL-31 at between 11 picograms per ml and 22 picograms per ml, IL-4 at between 17 picograms per ml and 33 picograms per ml, IL-5 at between 15 picograms per ml and 28 picograms per ml, IL-6 at between 50 picograms per ml and 95 picograms per ml, IL-7 at between 26 picograms per ml and 50 picograms per ml, IL-8 at between 4504 picograms per ml and 8366 picograms per ml, IL-9 at between 131 picograms per ml and 245 picograms per ml, IP-10 at between 469 picograms per ml and 873 picograms per ml, LIF at between 41 picograms per ml and 77 picograms per ml, MCP-1 at between 14,480 picograms per ml and 26,893 picograms per ml, MIP-la at between 303 picograms per ml and 565 picograms per ml, ΜΙΡ-Ιβ at between 375 picograms per ml and 698 picograms per ml, PDGF-BB at between 807 picograms per ml

WO 2018/226435

PCT/US2018/034584 and 1500 picograms per ml, PIGF-1 at between 743 picograms per ml and 1381 picograms per ml, RANTES at between 3277 picograms per ml and 6087 picograms per ml, SCF at between 23 picograms per ml and 45 picograms per ml, SDF-Ια at between 1114 picograms per ml and 2071 picograms per ml, TNF- a at between 42 picograms per ml and 80 picograms per ml, TNF-β at between 12 picograms per ml and 25 picograms per ml, VEGFA at between 481 picograms per ml and 895 picograms per ml, and VEGF-D at between 46 picograms per ml and 88 picograms per ml.
17. The composition of claim 16, wherein the concentrations of the cytokines are: BDNF at between 527 picograms per ml and 880 picograms per ml, bNGF at between 15 picograms per ml and 25 picograms per ml, EGF at between 766 picograms per ml and 1278 picograms per ml, Eotaxin at between 39 picograms per ml and 67 picograms per ml, FGF-2 at between 30 picograms per ml and 51 picograms per ml, GM-CSF at between 8 picograms per ml and 15 picograms per ml, Gro-α at between 3186 picograms per ml and 5312 picograms per ml, HGF at between 378 picograms per ml and 631 picograms per ml, IFN-a at between 271 picograms per ml and 454 picograms per ml, IFN-γ at between 35 picograms per ml and 60 picograms per ml, IL-10 at between 72 picograms per ml and 121 picograms per ml, IL-12p70 at between 10 picograms per ml and 18 picograms per ml, IL-13 at between 12 picograms per ml and 21 picograms per ml, IL-15 at between 32 picograms per ml and 55 picograms per ml, IL-17A at between 21 picograms per ml and 35 picograms per ml, IL-18 at between 83 picograms per ml and 140 picograms per ml, IL-Ια at between 763 picograms per ml and 1273 picograms per ml, IL-Ιβ at between 15 picograms per ml and 27 picograms per ml, IL-IRA at between 2373 picograms per ml and 3956 picograms per ml, IL-2 at between 17 picograms per ml and 30 picograms per ml, IL-21 at between 396 picograms per ml and 662 picograms per ml, IL-22 at between 17 picograms per ml and 30 picograms per ml, IL-23 at between 216 picograms per ml and 361 picograms per ml, IL-27 at between 22 picograms per ml and 38 picograms per ml, IL-31 at between 12 picograms per ml and 21 picograms per ml, IL-4 at between 18 picograms per ml and 32 picograms per ml, IL-5 at between 16 picograms per ml and 27 picograms per ml, IL-6 at between 54 picograms per ml and 91 picograms per ml, IL-7 at between 28 picograms per ml and 48 picograms per ml, IL-8 at between 4826 picograms per ml and 8044 picograms per ml, IL-9 at between 141 picograms per ml and 236 picograms per ml, IP-10 at between 503 picograms per ml and 840 picograms per ml, LIF at between 44 picograms per ml and 74 picograms per ml, MCP-1 at between 15,514 picograms per ml and 25,859 picograms per ml, MIP-la at between 325 picograms per ml and 543 picograms per ml, ΜΙΡ-Ιβ at between 402 picograms per ml and 671

WO 2018/226435

PCT/US2018/034584 picograms per ml, PDGF-BB at between 865 picograms per ml and 1442 picograms per ml, PIGF-1 at between 796 picograms per ml and 1328 picograms per ml, RANTES at between 3511 picograms per ml and 5853 picograms per ml, SCF at between 25 picograms per ml and 43 picograms per ml, SDF-Ια at between 1194 picograms per ml and 1991 picograms per ml, TNF- a at between 45 picograms per ml and 77 picograms per ml, TNF-β at between 13 picograms per ml and 24 picograms per ml, VEGF-A at between 516 picograms per ml and 861 picograms per ml, and VEGF-D at between 50 picograms per ml and 84 picograms per ml.
18. The composition of claim 16, wherein the concentrations of the cytokines are: BDNF at between 562 picograms per ml and 844 picograms per ml, bNGF at between 16 picograms per ml and 24 picograms per ml, EGF at between 817 picograms per ml and 1227 picograms per ml, Eotaxin at between 42 picograms per ml and 64 picograms per ml, FGF-2 at between 32 picograms per ml and 49 picograms per ml, GM-CSF at between 9 picograms per ml and 14 picograms per ml, Gro-α at between 3399 picograms per ml and 5099 picograms per ml, HGF at between 403 picograms per ml and 605 picograms per ml, IFN-a at between 290 picograms per ml and 436 picograms per ml, IFN-γ at between 38 picograms per ml and 57 picograms per ml, IL-10 at between 77 picograms per ml and 117 picograms per ml, IL-12p70 at between 11 picograms per ml and 18 picograms per ml, IL-13 at between 13 picograms per ml and 20 picograms per ml, IL-15 at between 34 picograms per ml and 53 picograms per ml, IL-17A at between 22 picograms per ml and 34 picograms per ml, IL-18 at between 89 picograms per ml and 135 picograms per ml, IL-la at between 814 picograms per ml and 1222 picograms per ml, IL-ΐβ at between 17 picograms per ml and 26 picograms per ml, IL-IRA at between 2531 picograms per ml and 3798 picograms per ml, IL-2 at between 18 picograms per ml and 29 picograms per ml, IL-21 at between 423 picograms per ml and 636 picograms per ml, IL-22 at between 19 picograms per ml and 29 picograms per ml, IL-23 at between 230 picograms per ml and 347 picograms per ml, IL-27 at between 24 picograms per ml and 37 picograms per ml, IL-31 at between 13 picograms per ml and 20 picograms per ml, IL-4 at between 19 picograms per ml and 30 picograms per ml, IL-5 at between 17 picograms per ml and 26 picograms per ml, IL-6 at between 58 picograms per ml and 88 picograms per ml, IL-7 at between 30 picograms per ml and 46 picograms per ml, IL-8 at between 5147 picograms per ml and 7722 picograms per ml, IL-9 at between 150 picograms per ml and 226 picograms per ml, IP-10 at between 537 picograms per ml and 806 picograms per ml, LIF at between 47 picograms per ml and 71 picograms per ml, MCP-1 at between 16,549 picograms per ml and 24,824 picograms per ml, MIP-la at between 347 picograms

WO 2018/226435

PCT/US2018/034584 per ml and 522 picograms per ml, ΜΙΡ-Ιβ at between 429 picograms per ml and 644 picograms per ml, PDGF-BB at between 922 picograms per ml and 1385 picograms per ml, PIGF-1 at between 849 picograms per ml and 1275 picograms per ml, RANTES at between 3745 picograms per ml and 5619 picograms per ml, SCF at between 27 picograms per ml and 42 picograms per ml, SDF-Ια at between 1273 picograms per ml and 1911 picograms per ml, TNF- a at between 49 picograms per ml and 74 picograms per ml, TNF-β at between 14 picograms per ml and 23 picograms per ml, VEGF-A at between 550 picograms per ml and 826 picograms per ml, and VEGF-D at between 53 picograms per ml and 81 picograms per ml.
19. The composition of claim 16, wherein the concentrations of the cytokines are: BDNF at between 597 picograms per ml and 809 picograms per ml, bNGF at between 17 picograms per ml and 23 picograms per ml, EGF at between 868 picograms per ml and 1176 picograms per ml, Eotaxin at between 45 picograms per ml and 61 picograms per ml, FGF-2 at between 34 picograms per ml and 47 picograms per ml, GM-CSF at between 9 picograms per ml and 13 picograms per ml, Gro-α at between 3611 picograms per ml and 4887 picograms per ml, HGF at between 428 picograms per ml and 580 picograms per ml, IFN-a at between 308 picograms per ml and 418 picograms per ml, IFN-γ at between 40 picograms per ml and 55 picograms per ml, IL-10 at between 82 picograms per ml and 112 picograms per ml, IL-12p70 at between 12 picograms per ml and 17 picograms per ml, IL-13 at between 13 picograms per ml and 19 picograms per ml, IL-15 at between 36 picograms per ml and 51 picograms per ml, IL-17A at between 23 picograms per ml and 33 picograms per ml, IL-18 at between 95 picograms per ml and 129 picograms per ml, IL-la at between 865 picograms per ml and 1171 picograms per ml, IL-Ιβ at between 18 picograms per ml and 25 picograms per ml, IL-IRA at between 2689 picograms per ml and 3640 picograms per ml, IL-2 at between 20 picograms per ml and 28 picograms per ml, IL-21 at between 449 picograms per ml and 609 picograms per ml, IL-22 at between 20 picograms per ml and 28 picograms per ml, IL-23 at between 245 picograms per ml and 332 picograms per ml, IL-27 at between 25 picograms per ml and 35 picograms per ml, IL-31 at between 13 picograms per ml and 19 picograms per ml, IL-4 at between 21 picograms per ml and 29 picograms per ml, IL-5 at between 18 picograms per ml and 25 picograms per ml, IL-6 at between 61 picograms per ml and 84 picograms per ml, IL-7 at between 32 picograms per ml and 44 picograms per ml, IL-8 at between 5469 picograms per ml and 7401 picograms per ml, IL-9 at between 160 picograms per ml and 217 picograms per ml, IP-10 at between 570 picograms per ml and 772 picograms per ml, LIF at between 50 picograms per ml and 68 picograms per ml, MCP-1 at between

WO 2018/226435

PCT/US2018/034584

17,583 picograms per ml and 23,790 picograms per ml, MIP-la at between 369 picograms per ml and 500 picograms per ml, ΜΙΡ-Ιβ at between 455 picograms per ml and 617 picograms per ml, PDGF-BB at between 980 picograms per ml and 1327 picograms per ml, PIGF-1 at between 902 picograms per ml and 1222 picograms per ml, RANTES at between 3979 picograms per ml and 5385 picograms per ml, SCF at between 29 picograms per ml and 40 picograms per ml, SDF-Ια at between 1353 picograms per ml and 1832 picograms per ml, TNF- a at between 52 picograms per ml and 71 picograms per ml, TNF-β at between 15 picograms per ml and 22 picograms per ml, VEGF-A at between 585 picograms per ml and 792 picograms per ml, and VEGF-D at between 56 picograms per ml and 78 picograms per ml.
20. The composition of claim 16, wherein the concentrations of the cytokines are: BDNF at between 632 picograms per ml and 774 picograms per ml, bNGF at between 18 picograms per ml and 22 picograms per ml, EGF at between 919 picograms per ml and 1124 picograms per ml, Eotaxin at between 47 picograms per ml and 59 picograms per ml, FGF-2 at between 36 picograms per ml and 45 picograms per ml, GM-CSF at between 10 picograms per ml and 13 picograms per ml, Gro-α at between 3823 picograms per ml and 4674 picograms per ml, HGF at between 453 picograms per ml and 555 picograms per ml, IFN-a at between 326 picograms per ml and 399 picograms per ml, IFN-γ at between 42 picograms per ml and 53 picograms per ml, IL-10 at between 87 picograms per ml and 107 picograms per ml, IL-12p70 at between 12 picograms per ml and 16 picograms per ml, IL-13 at between 14 picograms per ml and 19 picograms per ml, IL-15 at between 39 picograms per ml and 48 picograms per ml, IL-17A at between 25 picograms per ml and 31 picograms per ml, IL-18 at between 100 picograms per ml and 124 picograms per ml, IL-la at between 916 picograms per ml and 1121 picograms per ml, IL-Ιβ at between 19 picograms per ml and 24 picograms per ml, IL-IRA at between 2848 picograms per ml and 3481 picograms per ml, IL-2 at between 21 picograms per ml and 26 picograms per ml, IL-21 at between 476 picograms per ml and 583 picograms per ml, IL-22 at between 21 picograms per ml and 27 picograms per ml, IL-23 at between 259 picograms per ml and 318 picograms per ml, IL-27 at between 27 picograms per ml and 34 picograms per ml, IL-31 at between 14 picograms per ml and 18 picograms per ml, IL-4 at between 22 picograms per ml and 28 picograms per ml, IL-5 at between 19 picograms per ml and 24 picograms per ml, IL-6 at between 65 picograms per ml and 81 picograms per ml, IL-7 at between 34 picograms per ml and 42 picograms per ml, IL8 at between 5791 picograms per ml and 7079 picograms per ml, IL-9 at between 169 picograms per ml and 208 picograms per ml, IP-10 at between 604 picograms per ml and 739

WO 2018/226435

PCT/US2018/034584 picograms per ml, LIF at between 53 picograms per ml and 66 picograms per ml, MCP-1 at between 18,617 picograms per ml and 22,756 picograms per ml, MIP-la at between 390 picograms per ml and 478 picograms per ml, ΜΙΡ-Ιβ at between 482 picograms per ml and 590 picograms per ml, PDGF-BB at between 1038 picograms per ml and 1269 picograms per ml, PIGF-1 at between 955 picograms per ml and 1169 picograms per ml, RANTES at between 4213 picograms per ml and 5151 picograms per ml, SCF at between 30 picograms per ml and 38 picograms per ml, SDF-Ια at between 1433 picograms per ml and 1752 picograms per ml, TNF- a at between 55 picograms per ml and 68 picograms per ml, TNF-β at between 16 picograms per ml and 21 picograms per ml, VEGF-A at between 619 picograms per ml and 758 picograms per ml, and VEGF-D at between 60 picograms per ml and 74 picograms per ml.
21. The composition of claim 16, wherein the concentrations of the cytokines are: BDNF at between 668 picograms per ml and 739 picograms per ml, bNGF at between 19 picograms per ml and 21 picograms per ml, EGF at between 970 picograms per ml and 1073 picograms per ml, Eotaxin at between 50 picograms per ml and 56 picograms per ml, FGF-2 at between 38 picograms per ml and 43 picograms per ml, GM-CSF at between 10 picograms per ml and 12 picograms per ml, Gro-α at between 4036 picograms per ml and 4462 picograms per ml, HGF at between 478 picograms per ml and 530 picograms per ml, IFN-a at between 344 picograms per ml and 381 picograms per ml, IFN-γ at between 45 picograms per ml and 50 picograms per ml, IL-10 at between 91 picograms per ml and 102 picograms per ml, IL-12p70 at between 13 picograms per ml and 15 picograms per ml, IL-13 at between 15 picograms per ml and 18 picograms per ml, IL-15 at between 41 picograms per ml and 46 picograms per ml, IL-17A at between 26 picograms per ml and 30 picograms per ml, IL-18 at between 106 picograms per ml and 118 picograms per ml, IL-la at between 967 picograms per ml and 1070 picograms per ml, IL-Ιβ at between 20 picograms per ml and 23 picograms per ml, IL-IRA at between 3006 picograms per ml and 3323 picograms per ml, IL-2 at between 22 picograms per ml and 25 picograms per ml, IL-21 at between 502 picograms per ml and 556 picograms per ml, IL-22 at between 22 picograms per ml and 26 picograms per ml, IL-23 at between 274 picograms per ml and 303 picograms per ml, IL-27 at between 28 picograms per ml and 32 picograms per ml, IL-31 at between 15 picograms per ml and 18 picograms per ml, IL-4 at between 23 picograms per ml and 27 picograms per ml, IL-5 at between 20 picograms per ml and 23 picograms per ml, IL-6 at between 69 picograms per ml and 77 picograms per ml, IL-7 at between 36 picograms per ml and 40 picograms per ml, IL8 at between 6113 picograms per ml and 6757 picograms per ml, IL-9 at between 178

WO 2018/226435

PCT/US2018/034584 picograms per ml and 198 picograms per ml, IP-10 at between 637 picograms per ml and 705 picograms per ml, LIF at between 56 picograms per ml and 63 picograms per ml, MCP-1 at between 19,652 picograms per ml and 21,721 picograms per ml, MIP-Ια at between 412 picograms per ml and 456 picograms per ml, ΜΙΡ-Ιβ at between 509 picograms per ml and 564 picograms per ml, PDGF-BB at between 1095 picograms per ml and 1212 picograms per ml, PIGF-1 at between 1009 picograms per ml and 1116 picograms per ml, RANTES at between 4447 picograms per ml and 4916 picograms per ml, SCF at between 32 picograms per ml and 36 picograms per ml, SDF-Ια at between 1512 picograms per ml and 1672 picograms per ml, TNF- a at between 58 picograms per ml and 65 picograms per ml, TNF-β at between 17 picograms per ml and 20 picograms per ml, VEGF-A at between 653 picograms per ml and 723 picograms per ml, and VEGF-D at between 63 picograms per ml and 71 picograms per ml.
22. The composition of claim 1, wherein the fluid comprising dextrose is a low pH fluid.
23. The composition of claim 1, wherein the fluid comprising dextrose further comprises dextran.
24. The composition of claim 1, wherein the protectant comprises albumin.
25. The composition of claim 10, wherein the fluid comprising dextrose is a low pH fluid.
26. The composition of claim 10, wherein the fluid comprising dextrose further comprises dextran.
27. The composition of claim 10, wherein the protectant comprises albumin.
28. The composition of claim 16, wherein the fluid comprising dextrose is a low pH fluid.
29. The composition of claim 16, wherein the fluid comprising dextrose further comprises dextran.
30. The composition of claim 16, wherein the protectant comprises albumin.