AU2018202720A1 - Method for determining the balance of the macro- and micronutrients in the body and system for implementation thereof - Google Patents

Abstract

Abstract METHOD FOR DETERMINING THE BALANCE OF THE MACRO- AND MICRONUTRIENTS IN THE BODY AND SYSTEM FOR IMPLEMENTATION The invention relates to the medical field and can be used for determining the balance of the macro- and micronutrients in the body and diagnosing various diseases on this basis. Spirit of the invention - the content of the macro- and micronutrients in a biosubstrate is determined, hair and nails of the patient being advantageously used as the biosubstrate, wherein content of the macro- and micronutrients and correlations thereof are defined, and the changes with respect to the norm of the content of the macro- and micronutrients are registered based on the values of the absolute deviations from the norm, and are used for referring the patient to the risk group with associated disease, furthermore, the changes with respect to the norm of the content for the macro- and micronutrients are further determined based on the values of the relative deviations from the norm at ratios of pairs of the macro- and micronutrients. System for implementing the method comprises a biosubstrate input device, a data processing unit, two computers, an ion source, a mass analyzer, as well as a pumping device. 2 independent claims, 3 dependent claims, 12 figures Start Measure the content Determine spectrometrically the of the elements content of macro- and micronutrients 3 N - the number of elements as a whole Initial Ne - the number of essential elements (E) data of N Ne - the number of conditionally essential elements elements N - the number of conditionally toxic elements (CT) Nee - the number of ratio of the E/E elements 4'' Nct/e - the number of ratio of the CT/E elements Receive the initial data -- of the analysis of the Array E[1..N] - name of the measured element x[1.N] E1..N] Array x[1..N] - value of the measured element Cycle i = 1 to Cycle i = 1 s E[1 YES to Neie conditionally oxic? 10 NO Calculation No. 3 Calculation Calculation N.Z No. No. 211 Cycle i - 1 to Ncu/e Calculation No. 4 Create the result table of the calculations No. 1-4 Output the result table (End Fig. 3

Description

METHOD FOR DETERMINING THE BALANCE OF THE MACRO- AND MICRONUTRIENTS IN THE BODY AND SYSTEM FOR IMPLEMENTATION THEREOF

The invention relates to medical field and can be used for determining the balance of the macro- and micronutrients in the body and diagnosing various diseases on this basis.

The macro- and micronutrients are components of various biological structures, in particular, active centres of certain enzymes, they take part in the most important biochemical processes, oxidation-reduction reactions, free-radical oxidation, protein synthesis, tissue differentiation and growth, interaction with intrinsic acids and monomers contained therein.

The concentration of the macro- and micronutrients in the healthy body is enough strictly balanced and maintained by homeostasis. Kinetics, distribution and depositing of metal ions are submitted to the biochemical regulation of macroorganism. The concentration change of each macro- and micronutrient is interdependent. One can get an idea of metabolic changes, occurring with participation of metal-containing molecules based on the state of the ion balance in the biosubstrates.

Based on the content of the macro- and micronutrients in the biosubstrate one can perform correlation with some diseases [Epaymajibn H. BHyrpeHHiie 6one3HH. B 10 Kimrax. Kmira 2. M.: Me/nmHHa. 1993. — 544 c.; rn. 77, Hapyniemie

MeTaGojmnecKHx ΜΗκροοηβΜβΗτοΒ, c. 451-457)].

From the prior art it is known the method for examining the biological system of homeostasis of the macro- and micronutrients by determining the concentration of the macro- and micronutrients in hair [RU 2180121, Cl, G01N33/84, 27.02.2002], characterized in that, the content of the macro- and micronutrients is further determined in blood serum, and diagnostic features typical for each nosology are determined based on both quantitative change of the concentration of the macro- and micronutrients and change of the correlations between the macro- and micronutrients with respect to the norm.

Furthermore, the data are used of that the deficiency of zinc in hair, manganese and magnesium in blood serum, the excess of zinc in blood serum, as well as changing in system of linear correlation ratios of the content of manganese, magnesium, copper, iron, phosphorus and zinc in blood serum, zinc, copper, manganese, magnesium and phosphorus in hair are diagnostic features of diabetes mellitus; the deficiency of copper in blood serum and hair, deficiency of manganese in blood serum, increase of zinc concentration in blood serum, increase of manganese, molybdenum, cadmium concentration in hair, as well as changing in systems of linear correlations among the concentrations of copper, iron, magnesium, manganese, molybdenum in blood serum, cobalt, phosphorus, molybdenum, copper, magnesium in hair with respect to the norm are diagnostic features of thyroid hyperplasia; the increase of copper, phosphorus, zinc concentration in blood serum, the decrease of sulphur concentration in blood serum, the small decrease of iron concentration in hair, as well as changing in system of linear correlations among the concentrations of copper, iron, magnesium, manganese, zinc in blood serum, zinc, manganese, sulphur, phosphorus, molybdenum, magnesium, iron, copper, cobalt in hair with respect to the norm are diagnostic features of rheumatoid arthritis.

The disadvantage of the method consists in relatively high complexity and relatively low efficiency thereof, caused by the need of additional determining the content of the macro- and micronutrients in blood serum.

It is also known the method [RU 2230491, C2, A61B 6/00, G01N 33/84, 20.06.2004], which consists in collecting the sample of hair and/or nails of the woman, in which the concentrations of pairs of the macro- and micronutrients K, Na, Cu, Fe, Mg, Pb, Cd, As, Se, Ca and Mg are determined, then the ratios of pair concentrations K/Na, Cu/Fe, Mg/Pb, Cd/Se, Ca/Mg, As/Se are calculated, and the woman is referred to the risk group when the deviations of concentration ratios from the norm of similar ratios of these pairs are detected.

The disadvantage of the method consists in relatively limited field of application, since it is intended only for detecting risk groups of breast diseases among women.

The method for determining the balance of the macro- and micronutrients in the body by detecting the content of the micronutrients in the biosubstrate [RU 2129426, Cl, A61K35/36, 27.04.1999 ] is the closest to the proposed one according to its technical essence and achieved result when using, the method comprises using the hair and nails as the biosubstrate, advantageously, wherein the content of the macro- and micronutrients Cu, Fe, Mg, P, Zn, Cd, Co, Mo, Pb, K, Ca, Se, St, Cr and correlations thereof are determined, and when the content of the micronutrients is changed with respect to the norm, the correlation alterations, which reflect ion balance in the body, define the correlation with various diseases.

Thus, the final step of the method consists in determining the disease (risk group), characterized by just such changes based on the data of changes with respect to the norm of the content of the micronutrients and the alteration of correlations.

In this regard, for example, it is used the information of that, in case of hypothyroidism the changes with respect to the norm in the content of the micronutrients Cu, Fe, Mg, Μη, P, Zn, Cd, Co, Mo and correlations thereof are detected, wherein in case of underactive thyroid gland significant differences (P < 0.05) in the content of Cd, Mn, Fe as compared with the norm, decrease of the amount of Cd and simultaneous change of correlations, emergence of positive correlation between Mn and Fe, increase of the content of P and emergence of negative correlation with Co (r - 0.76), decrease of the content of Mg, Mn, Mo are detected; in case of hyperthyroidism presence of high negative correlation coefficient (r - 0.90) between Zn and Fe, increase of the content of Mg, P and Mo are detected, in case of normal functional state of thyroid presence of negative correlation between Cd and P (r - 0.64) and positive one with Zn (r - 0.73), positive correlation between Mn and Cu (r - 0.76), Mg (r - 0.67) and negative one with P (r - 0.66) is detected. Furthermore, in case of connective tissue diseases of autoimmune nature significant decrease of copper, zinc, calcium, selenium is observed, in case of urolithiasis significant increase of cobalt, magnesium and phosphorus is observed, in case of sympathoadrenal crises, combined with neuroendocrinal metabolic form of diencephalic syndrome, significant increase of potassium, magnesium and decrease of manganese are observed, in case of allergodermathosis, combined with malabsorption syndrome, abrupt decrease of zinc, significant increase of lead and polymineral deficiency are observed, in case of osteoporosis of various ethiology significant increase of strontium, decrease of magnesium, potassium, manganese, zinc and copper are observed, in case of cardiovascular diseases significant decrease of potassium, magnesium, chrome and copper is observed, in case of bronchial asthma significant decrease of zinc, copper and magnesium is observed, in case of carbohydrate metabolism disorder decrease of chrome, potassium, manganese and significant decrease of zinc are observed.

The disadvantage of the closest technical result in respect to the method lies in relatively low accuracy and faithfulness of disease (risk group) determining. It is caused by using the information only about the absolute deviation of the content of the macro- and micronutrients from the norm and, at the same time, the information about the change of the relative content of the macro- and micronutrients in pairs is not taken into account, which, as determined when developing the proposed method, allows to detect the concealed balance alteration, and is typical for certain diseases.

The object of the present invention relative to the method lies in developing the method, characterized in higher accuracy and faithfulness of disease (risk group) determining, by taking into account not only absolute deviations from the norm of the content of the macro- and micronutrients in the body, but, at the same time, also deviations from the norm of ratios thereof.

The desired technical result to the method consists in increasing the determination accuracy of the disease (risk group) based on determining the balance of the macro-and micronutrients in the body.

The formulated problem to the method is solved, and the desired technical result is achieved by the method where the content of the macro- and micronutrients in the biosubstrate is determined, hair and nails of the patient being advantageously used as the biosubstrate, wherein the content of the macro- and micronutrients and correlations thereof are determined, and the changes with respect to the norm of the content of the macro- and micronutrients are registered based on the values of the absolute deviations from the norm, and used for referring the patient to the risk group with associated disease, according to the invention, the changes with respect to the norm of the content of the macro- and micronutrients are further determined based on the relative deviations from the norm at ratios of pairs of the macro- and micronutrients, wherein the acceptable intervals for absolute deviations from the norm of the content of the macro- and micronutrients and the acceptable intervals for the relative deviations from the norm at ratios of pairs of the macro- and micronutrients are determined, and the patient is referred to the risk group with associated disease based on the data of going beyond the acceptable intervals of the absolute deviations of the macro- and micronutrients and/or based on the data of going beyond the acceptable intervals of the relative deviations from the norm at ratios of pairs of the macro- and micronutrients, and/or based on the concealed deviation of one element of the pair beyond the acceptable intervals at ratios of pairs of the macro- and micronutrients.

Besides, the required technical solution is achieved by that the macro- and micronutrients the content of which is determined in the biosubstrate are the essential macronutrients Ca, Cl, F, K, Na, Mg, P and S, and the essential micronutrients Cr, Cu, Fe, I, Mn, Mo, Se, Zn as well as the conditionally essential micronutrients Ag, Al, Au, B, Br, Co, Ge, Li, Ni, Si, V and the conditionally toxic micronutrients As, Ba, Be, Bi, Cd, Ce, Cs, Dy, Er, Eu, Ga, Gd, Elf, Hg, Ho, In, Ir, La, Lu, Nb, Nd, Os, Pb, Pd, Pr, Pt, Rb, Re, Rh, Ru, Sb, Sc, Sm, Sn, Sr, Ta, Tb, Te, Th, Ti, Tl, Tm, U, W, Y, Yb, Zr.

Besides, the required technical solution is achieved by that the ratios of the essential macro- and micronutrient pairs are Ca/P, Na/K, Na/Mg, Ca/Mg, Fe/Cu, Zn/Cu, Ca/K, and of the conditionally toxic and essential elements are Pb/S, Pb/Fe, Pb/Ca, Cd/Zn, Cd/S, Hg/Z, Hg/Se, Hg/S, Hg/Zn.

Further, the required technical solution is achieved by that the normal content interval for the essential and conditionally essential macro- and micronutrients includes a zone of the lower limit of the interval, a zone of the guaranteed normal content and a zone of the upper limit of the interval and for the conditionally toxic micronutrients a zone of the guaranteed normal content and a zone of the upper limit of the interval.

In addition to the analogues of the proposed method described above, it is known systems and devices used in the quantitative and qualitative analysis of micronutrients for use in the diagnosis of diseases.

It is known a computer-aided device for diagnosis [RU 134412, Ul, A61B 1/04, 20.11.2013], comprising a lighting aperture with an eye lens and a field lens and a forceps aperture of the esophagogastroduodenoscope structurally combined, two groups of the optical fibers, a group of the controlled optical effect sources, a personal computer, an information output of which is an information output of the device, a color video camera, connected with the information outputs to the information inputs of the personal computer, a spectrometer, a pathological simulator and a health simulator, wherein the first control output of the personal computer is connected to the start input of the controlled optical effect source group and its outputs are optically connected to the inputs of the first group of the optical fibers, the outputs of which are the optical outputs of the device, the inputs of the second optical fiber group are the optical inputs of the device, the pathological simulator and the health simulator being configured to show respectively indications of the simulation of the different secondary fluorescent emission from pathology types and non-infected NDM-1 of the mucosa when supply to them the probing radiation from the radiation source, and to connect optically them by turn to the outputs of the first group optical fibers and to the inputs of the second group optical fibers, the outputs of the second group optical fibers are connected to the optical input of the spectrometer, information outputs of which through the USB-port are connected to the information inputs of the personal computer, the second and third control outputs of which are connected respectively to the start input of the spectrometer and to the start input of the color video camera, and the metallised nano-coatings are deposited on the outer side surface of the second group fibers.

The disadvantage of this technical solution are relatively narrow capabilities, since it is intended for diagnosis of the esophagus, stomach and duodenum, infected with the NDM-1 bacteria genes and their mutations, but it cannot be used to compare the results of determining the content of the macro- and micronutrients in various biosubstrates in order to reveal being some of them beyond the limits of permissible norms.

It is also known a system [RU 18206, Ul, G06F 17/40, 27.05.2001], which is an automated workstation (AWS), consisting of a set of personal computer equipment as a part of the connected with the interface system unit with an internal memory to enter a database, a keyboard, a monitor, a printer and a modem, wherein the memory includes a database of medical specialists, a database of patients, a database of history cases, a database of diagnostic criteria, a database of recommended means and methods of treatment, a video and graphic database, and AWS further includes instrumental diagnostic tools to receive the primary parameters of the patient's body condition, the interface devices to connect the instrumental diagnostic tools and the digital video camera, wherein the outputs of the instrumental diagnostic tools and video camera are connected through the corresponding interface devices to the system unit, and the inputs and outputs of the database of patients, database of history cases, database of diagnostic criteria, database of recommended means and methods of treatment and video and graphic database are connected to the corresponding outputs and inputs of the system unit through the AWS intrasystem bus.

The disadvantage of this technical solution is relatively narrow capabilities, since it cannot be used to compare the results of determining the content of the macro- and micronutrients in various biosubstrates in order to reveal being some of them beyond the limits of permissible norms.

From the state of art, the atomic absorption spectrometric systems, which include a source of electromagnetic radiation, a sample entry device, an atomizer, a monochromator, a detector and a recording device, are also known [Bcjihukhh B.H. OCHOBbI ΜβΤΟΛΟΒ aTOMHO-a6cOp6u,HOHHOH H aTOMHO-OMHCCHOHHOH CneKTpOCKOnHH.

Mmhck: ΒΓΜΥ, 2015, pnc. 3, c. 14, http://rep.bsmu.by/xmlui/bitstream/handle/BSMU/7723/365889-%D0%Bl%Dl%80.pdf?sequence=3&amp;isAllowed=y], and atomic emission spectrometric systems, which comprise a source of flame, an optical filter or monochromator, a detecting device and a recording device [in place cited, fig. 12, p. 33],

The disadvantage of such systems is a relatively low accuracy in determining the content of chemical elements and limited capabilities, which makes it impossible to compare the results of the determining of the content of the macro- and micronutrients in various biosubstrates.

The closest to the claimed system is a mass spectrometric system [Xhmhk. Macc-ΰπεκτροΜετρη». http://www.xumuk.ru/encyklopedia/2448.html)], comprising an input device, an ion source, a mass analyzer, a pumping source, a detector, a data processing system and a computer, a more detailed description of which is presented in [Xhmhk. Macc-cneKTpoMeTpw. http://www.xumuk.ru/bse/1585.html].

Such a system has relatively narrow capabilities, which doesn’t allow, in particular, to compare the results of determining the content of the macro- and micronutrients in various biosubstrates in order to reveal being some of them beyond the limits of permissible norms. This is determined by that the system does not create a body mineralogram and does not determine the content of which chemical elements goes beyond the limits of normal values in a distinct or concealed form. This reduces the accuracy and sensitivity of the system for the subsequent use of this information for the diagnosis of diseases.

The problem to be solved according to the claimed invention with respect to the system is to increase the accuracy and sensitivity of the system by expanding its capabilities in order to allow creating a body mineralogram based on the measurements and to determine the content of which chemical elements goes beyond the normal values in a distinct or concealed form.

The technical result ensured by the given set of features with respect to the system is an increase in the accuracy and sensitivity when determining the content deviation of chemical elements in the biosubstrates of the human body (primarily in hair and nails) for the subsequent use of this information for the diagnosis of diseases.

The set problem is solved and the required technical solution is achieved by integrating to the spectrometer system for examining the body mineralogram in order to analyse hair or nails, comprising a biosubstrate input device, a data processing unit, a first computer, an ion source, the input of which is connected to the biosubstrate input device output, a mass analyzer, the input of which is connected to the ion source output, a detector, the input of which is connected to the first output of the mass analyzer, the first output is connected to the input of the data processing unit, the output of which is connected to the first input of the first computer, the second input of which is connected to the second output of the mass analyzer, as well as a pumping device, the first, second and third inputs of which are connected to the second outputs of the ion source and detector and to the third output of the mass analyzer, according to the invention, a reference interval parameter setting unit for individual elements, a centering and normalization unit for individual elements, the first input of which is connected to the output of the reference interval parameter setting unit for individual elements, and the second input is connected to the output of the first computer, a second computer, a decryption unit for individual elements, the input of which is connected to the output of the centering and normalizing unit for individual elements, and the output is connected to the first input of the second computer, a unit for calculating and comparing the distance to the upper and lower limits, the input of which is connected to the output of the centering and normalization unit 8 for individual elements and to the second input of the second computer, a unit for forming the ratios of the contents of the elements in a pair, the input of which is connected to the first computer output, a reference interval parameter setting unit for the pair ratios, a centering and normalization unit for the pair ratios, the first input is connected to the output of the reference interval parameter setting unit for the pair ratios, and the second input is connected to the output of the unit for forming the ratios of the contents of the elements in a pair, a pair ratio decryption unit, the input of which is connected to the output of the centering and normalization unit for the pair ratios and to the third input of the second computer, and a concealed deviation detection unit, the first input of which is connected to the pair ratio decryption unit, the second input is connected to the output of the unit for calculating and comparing the distance to the upper and lower limits, the third input is connected to the output of the decryption unit for individual elements, and the output is connected to the fourth input of the second computer.

The object of increasing the accuracy of the determining of the disease (risk group) on the basis of determining the balance of the macro- and micronutrients of the body is solved by analysing the balance of the macro- and micronutrients in the body by detecting the content of the macro- and micronutrients in the biosubstrate, in which the biosubstrate is essentially hair and nails, determining the content of the macro- and micronutrients and in case of change of the micronutrient content with respect to the norm - defining the correlation with various diseases. Besides, the correlation with various diseases is also defined in case of change the macronutrient content with respect to the norm, wherein the interval of the normal content is set for each macro-and micronutrient, and the change of the content of the macro- and micronutrients with respect to the norm means a state in which the result of determining the content of particular the macro- or micronutrient goes beyond the limit of the normal content for this macro- or micronutrient, in addition, the interval of the normal content is set for each selected content ratio of the macro- and micronutrients and when the value of any selected content ratio of the macro- and micronutrients goes beyond the interval of the normal content it is further detected for this ratio a change of the content of the macro-or micronutrients with respect to the content norm. Finally, the correlations with various diseases for each macro- or micronutrient are defined both through absolute deviations and through relative changes in the pairs of elements for simultaneous use in making judgement about the disease.

Ca, Cl, F, K, Na, Mg, P, S can be appreciated as the macronutrients, and as the micronutrients the other stable chemical elements, except for Ο, H, C, N, which are principal, wherein the macronutrients and Cr, Cu, Fe, I, Mn, Mo, Se, Zn relate to the essential elements, the micronutrients Ag, Al, Au, B, Co, Ge, Li, Si, V relate to the conditionally essential elements, and the other micronutrients relate to the conditionally toxic elements.

In turn, selected ratios of the content of the macro- and micronutrients can be appreciated as ratios of the content of the essential elements Ca/P, Ca/K, Na/K, Na/Mg, Ca/Mg, Fe/Cu, Zn/Cu, as well as the conditionally toxic and the essential elements Pb/S, Pb/Fe, Pb/Ca, Cd/Zn, Cd/S, Hg/Z, Hg/Se, Hg/S.

The normal content interval for the essential and conditionally essential elements can include a zone of the lower limit of the norm, a guaranteed normal content interval and a zone of the upper limit of the norm, and for the conditionally toxic elements - a guaranteed normal content interval and a zone of the upper limit of the norm.

The diagnosis of a variety of diseases can be performed based on the content of the macro- and micronutrients in hair and nails. Whereby it should be taken in account both changes with respect to the norm of the absolute content of the macro- and micronutrients, and changes with respect to the content of the macro- and micronutrient pairs, determined by comparing the selected ratio of the macro- and micronutrients with the limits of the corresponding intervals of the normal content.

The essence of the invention is disclosed with the help of drawings, wherein: fig. 1 is an illustration for detennining the interval limits for the elements having the upper and lower limits of the normal content (so called the essential and conditionally essential elements); fig. 2 is an illustration for determining the interval limits for the elements, the lower limit of the normal content of which coincides with 0 (so called the conditionally toxic elements); fig. 1 3 is an overall flow chart of the calculation algorithm; fig. 2 4 is a flow chart of the calculation algorithm for each of the essential and conditionally essential elements (calculation No. 1); fig. 3 5 is a flow chart of the calculation algorithm for each of the conditionally toxic elements (calculation No. 2); fig. 4 6 is a flow chart of the calculation algorithm for the ratio of the essential elements (calculation No. 3); fig. 7 is a flow chart of the calculation algorithm for the ratio of the essential elements (calculation No. 3.1); fig. 8 is a flow chart of the calculation algorithm for the ratio of the essential elements (calculation No. 3.2); fig. 9 is a flow chart of the calculation algorithm for the ratio of the essential elements (calculation No. 3.3); fig. 10 is a flow chart of the calculation algorithm for the ratio of the essential elements (calculation No. 3.4); fig. 11 is a flow chart of the calculation algorithm for the ratio of the conditionally toxic and essential elements (calculation No. JV2 4). fig. 12 is a structural diagram of the system for implementation of the method, which is a spectrometric system for examining the body mineralogram based on the analysis of hair or nails.

In fig. 12: 1 - biosubstrate input device, 2 - ion source, 3 - mass analyzer, 4 -detector, 5 - data processing unit, 6 - first computer, 7 - pumping device, 8 -centering and normalization unit for individual elements, 9 - unit for forming ratios of the element contents in a pair, 10 - centering and normalization unit for pair ratios, 11 - reference interval parameter setting unit for individual elements, 12 - reference interval parameter setting unit for pair ratios, 13 decryption unit for individual elements, 14 - unit for calculating and comparing the distance to upper and lower limits, 15 concealed deviation detection unit, 16 pair ratio decryption unit, 17 second computer, 18 evacuated part of the system, 19 determination channel of distinct deviations, 20 - determination channel of concealed deviations, 21 - ion beam transmission channel, 22 - feed channel of ions separated by mass, 23 - line of sending ion mass information, 24 - line of sending a signal with ion current information, 25 - line of sending ion current data, 26 - line of sending a signal about the measured content absolute value for individual elements, 27 - line of setting reference interval parameter for individual elements, 28 - line of sending a deviation signal for individual elements, 29 - line of sending information about the entering zone for individual elements, 30 line of sending a signal of the content ratio of elements in a pair, 31 - line of setting reference interval parameter for a pair, 32 - line of sending deviation signal for a pair, 33 - line of sending information about the entering zone for a pair, 34 - line of sending information about proximity to limits, 35 - line of sending information about presence of concealed deviations.

The spectrometric system for examining the body mineralogram based on the analysis of hair and nails, which implements the proposed method, comprises a biosubstrate input device 1, a data processing unit 5 and a first computer 6, as well as an ion source 2, the input of which is connected to the biosubstrate input device 1 output, a mass analyzer 3, the input of which is connected to the ion source 2 output, a detector 4, the input of which is comiected to the first output of the mass analyzer 3, the first output is connected to the input of the data processing unit 5, the output of which is connected to the first input of the first computer 6, the second input of which is connected to the second output of the mass analyzer 3, a pumping device 7, the first, second and third inputs of which are connected to the second outputs of the ion source 2 and the detector 4 and to the third output of the mass analyzer 3, a reference interval parameter setting unit 11 for individual elements, a centering and normalization unit 8 for individual elements, the first input of which is connected to the output of the reference interval parameter setting unit 11 for individual elements, and the second input is connected to the output of the first computer 6, a second computer 17, a decryption unit 13 for individual elements, the input of which is connected to the output of the centering and normalizing unit 8 for individual elements, and the output is connected to the first input of the second computer 17, a unit 14 for calculating and comparing the distance to the upper and lower limits, the input of which is connected to the output of the centering and normalization unit 8 for individual elements and to the second input of the second computer 17, a unit 9 for forming the ratios of the contents of the elements in a pair, the input of which is connected to the first computer 6 output, a reference interval parameter setting unit 12 for the pair ratios, the centering and normalization unit 10 for the pair ratios, the first input is connected to the output of the reference interval parameter setting unit 12 for the pair ratios, the second input is connected to the output of the unit 9 for forming the ratios of the contents of the elements in a pair, a pair ratio decryption unit 16, the input of which is connected to the output of the centering and normalization unit 10 for the pair ratios and to the third input of the second computer 17, and a concealed deviation detection unit 15, the first input of which is connected to the pair ratio decryption unit 16, the second input is connected to the output of the unit 14 for calculating and comparing the distance to the upper and lower limits, the third input is connected to the output of the decryption unit 13 for individual elements, and the output is connected to the fourth input of the second computer 17.

In fig. 1 and fig. 2 the following designations are used: x - measured absolute value of the element content, pg/g;

Xm = (xu - xi)/2 is a mean value (middle point) of the reference interval (RI), wherein xu - upper limit of the reference interval, and xl - lower limit of the reference interval; / is a half-width of the reference interval (for the conditionally toxic elements the lower limit of the reference interval coincides with 0, as opposed to the essential and conditionally essential elements, for which this is not observed); y = (x - xm)// is a normalized and centered value of the element content; L - biologically admissible level; a - zone width of the upper limit of the norm (ULN), and in case of the essential and conditionally essential elements - also the lower limit of the norm (LLN); d is an "extreme" value (how far upper and lower limits of the norm go beyond the limits of the reference interval; the amount of this value can be set based on the analysis of measurement error, e.g., percentage units or put equal to zero).

In Table 1 and 2 the explanations for fig. 1 and fig. 2, accordingly, are given.

Table 1

Explanations to the graphs of the deviations of the essential and conditionally essential elements

Table 2

Explanations to the graphs of the deviations of the conditionally toxic elements

It should be noted that Ca, Cl, F, K, Na, Mg, P, S are appreciated as the macronutrients, and the other stable chemical elements are appreciated as the micronutrients, except for Ο, H, C, N, which are principal, wherein the macronutrients and Cr, Cu, Fe, I, Mn, Mo, Se, Zn relate to the essential elements, the micronutrients Ag, Al, Au, B, Co, Ge, Li, Si V relate to the conditionally essential elements, and the other micronutrients relate to the conditionally toxic elements, namely As, Ba, Be, Bi, Cd, Ce, Cs, Dy, Er, Eu, Ga, Gd, Hf, Hg, Ho, In, Ir, La, Lu, Nb, Nd, Os, Pb, Pd, Pr, Pt, Rb, Re, Rh, Ru, Sb, Sc, Sm, Sn, Sr, Ta, Tb, Te, Th, Ti, Tl, Tm, U, W, Y, Yb, Zr. Selected ratios of the content of the macro- and micronutrients are appreciated as ratios Ca/P, Ca/K, Na/K, Na/Mg, Ca/Mg, Fe/Cu, Zn/Cu, Pb/S, Pb/Fe, Pb/Ca, Cd/Zn, Cd/S, Hg/Z, Hg/Se, Hg/S.

The provided method is implemented as follows.

In the following description of the possible implementation of the method the following terms are used:

The antagonism of the elements is such a relationship between the elements, whereby increasing the content of one element typically is followed by decreasing the content of or uptaking the other element.

The biologically admissible level (BAL) is a limiting level of the element content in the body, after exceeding which organic changes in tissues are possible. Typically, the biologically admissible level several times higher than the upper limit of the reference interval. By now BAL is set not for all micronutrients.

The upper limit of the norm (ULN) is an area adjacent to the upper limit of the reference interval (takes about 1-20% of the reference interval width) and located generally within this interval typically with extremely low (maximum 5%) extreme in the outer area.

Above ULN, times - is a ratio of the upper limit of the reference interval to the measured value of the element content. If the element content is above the upper limit of the reference interval, this value will be > 1. In case of conditionally toxic elements this value coincides with deviation.

The limit of the reference interval is a value of the content element corresponding to the limit of the reference interval (upper or lower).

The deficiency (D) of the element means that the element content is below the lower limit of the norm (LLN).

Substantial content (SC) is the content of certain conditionally toxic elements with known BAL (Pb, Sr, Cd, Ti), for which when calculating the distance from 0 not to the upper limit of the reference interval, but to the biologically admissible level is taken as a unit of calculation. Such a content is always higher than the upper limit of the reference interval, but is below BAL.

The excess (E) of the element means that the element content is above ULN. There are 5 degrees of excess: mild degree, excess without indication of degree, moderate severity, pronounced excess and excess above biologically admissible level, BAL (for the elements, for which BAL is already set). In some cases, to designate excess terms "metabolic disorder", "excessive accumulation", "noticeable content" are used.

The guaranteed normal content interval (GNC1) is an interval covering the part of the reference interval, which does not include zones of the upper and lower limits of the norm.

The normal content interval is an interval, including the guaranteed normal content interval, as well as upper and lower limits of the norm. The output of the measured content value (element or element ratio) beyond the interval indicates the norm alteration.

The macronutrients are the elements, contained in relatively large quantities (>10 2%), these are Ca, Cl, L, K, Mg, Na, P, S. Together with principal elements in sum they form a group of the structural elements.

The micronutrients are the group of the chemical elements (in total 69), which are contained in the human body in very small quantities, within (10"M012) %.

The metabolic disorder (MD) is a state in which the content value of the essential or conditionally essential element is below LLN or above ULN, and one of the conditionally toxic element is above the upper limit of the norm. Lor the macronutrients typically the term "metabolic disorder" is applied to designate excess only.

The metabolic disorder of any essential or conditionally essential element can be characterized by two values: • deviation (from the middle of the reference interval for essential and conditionally essential elements and with respect to the upper limit of the RI - for conditionally toxic elements); • below LLN (above ULN), times.

The need to use two such numerical values simultaneously is determined by the fact that all studied elements have different width of the reference interval with respect to the middle of this interval, as well as different width of the LLN and ULN with respect to the width of the reference interval.

In conditionally toxic elements the lower limit of the reference interval coincides with 0, therefore only one value is used for them - above ULN, times (it numerically coincides with deviation with respect to 0).

Entering in LLN and ULN zones can typically not be considered as a metabolic disorder (deficiency or excess), but the fact of such entering enforces to follow dynamics of the content of the corresponding elements in the future.

Below LLN, times is a ratio of the lower limit of the reference interval to the measured value of the element content. If the element content is below the lower limit of the reference interval, this value will be > 1. This value is used only for essential and conditionally essential elements.

The lower limit of the norm (LLN) is an area adjacent to the lower limit of the reference interval (taking about 1-20% of the reference interval width) and located generally within this interval typically with extremely low (about 5%) extreme in the outer area.

The principal elements are C, Η, Ο, N.

Deviation: a) in case of the essential and conditionally essential elements, the distance from the result of measurement to the middle of the reference interval, if we assume that the half-width of the reference interval equal to one (in this case the lower limit of the reference interval corresponds to deviation - 1 and the upper limit, accordingly, 1). When the reference interval goes beyond the limits deviation is < -1 (below the lower limit) or > +1 (above the upper limit); in this case sometimes it is said "deviation with respect to the middle of the reference interval"; b) in case of conditionally toxic elements the distance from the result of measurement to zero, when assuming that the width of the reference interval is equal to 1. By sense in this case it is a deviation with respect to 0, it coincides with the measure "above ULN, times" and is always > 0.

The ratio of the element content is the ratio of values of the content of two elements. The analysis of such ratio enables detection of concealed abnormalities for one of two elements of the given pair. Typically, there is a ratio of the synergism or antagonism type in these pairs. The record of the type A/B means the ratio of the content of element A to the content of element B in the same biological sample.

The detection threshold (DT) is the minimum level, at which the presence of said element in the biosubstrate is detected. Sometimes really the element content is lower than the detection threshold. In this case the result of measurement is changed for numerical value of the detection threshold.

The reference interval (RI) is the interval of the element content of the entirely healthy person without predicting a deterioration in the state of health. Limits of the reference intervals are published in several sources, e.g., [PycTeMdeKOBa, C.A. Age- and sex-related differences in Al, Cd, Co, Cu, Fe, Mg, Μη, Mo, P, Pb, S and Zn levels in heard hair of health humans. Macro and Trace Elements. / S. Rustembekova, V. Zaichick // Mengen - und Spurenelemente. 22th Workshop.Friedrich-Schiller-Universitat, Jena. -2006. - Vol. 1. - P. 230-236; Pedpoe Β.Γ., TpoMoea O.A. BnTaMHHbi n MHKpoojieMeHTbi. - Μ.: «ΑΙ1ΕΦ-Β», 2003. - C. 626-633].

The synergism of the elements is such a relationship between the elements, whereby increasing the content of one element typically is followed by increasing the content of or uptaking the other element.

The element content in hair or nails is measured in pg/g, e.g.: the content of phosphorus is 100 pg/g means that 1 g of hairs comprises 100 pg of phosphorus.

As for the conditionally toxic (CT) micronutrients, the usage of such elements typically is harmful, than healthful, in total 50 items. These are all micronutrients, except for essential and conditionally essential elements. It is considered that mercury (Hg) is harmful for a human in any quantity, therefore it can be called as (unconditionally) toxic element, though it is included in mentioned 50 conditionally toxic micronutrients.

The conditionally essential (CE) micronutrients are the micronutrients that are vital, but harmful in certain dozes (Ag, Al, Au, B, Br, Co, Ge, Li, Ni, Si, V) - 11 items.

The essential (E) elements are vital elements; these are all structural elements (H, O, N, C; Ca, Cl, F, K, Mg, Na, P, S) + 8 micronutrients (Cr, Cu, Fe, 1, Mn, Mo, Se, Zn) -in total 20 items.

Besides the content of individual macro- and micronutrients in the body, the object of the interest is to study the ratio of various macro- and micronutrients, which may be in the state of synergism and/or antagonism (relative to each other). Information about these states for the most studied pairs of essential elements, as well as conditionally toxic and essential elements are shown in Table 3. E/E, as used herein, means the ratio of the content of two essential elements, and CT/E is the ratio of the content of the essential and conditionally toxic elements. A selection of the pairs of the macro- and micronutrients in order to form the ratio of their content, is determined by the available information about their effect on human health, described in various sources [PeopoB B.E., TpoMOBa O.A. Βητβμηηβι h MHKpoajieMeHTbi. - Μ.: «ΑΛΕΦ-Β», 2003. - 670 c.; Αβπ,μη Α.Π. and etc. MHKpo3JieMeHT03bi nenoBeKa: οτηολογη}!, KJiaccH(|)HKanHfl, opraHonaTOJiorna / Α.Π. Αβρμη, A.A. TKaBopomcoB, M.A. Pnm, JI.C. CTponKOBa; AMH CCCP. - M.: Me^nunHa, 1991. - 496 c.]/. Such ratios are used during the research in various laboratories, in particular, they are listed on the site of the company Trace Elements, USA (http://www.traceelements.com/docs/Graph.pdf, application date 01.06.2016).

Table 3

The relationship states for the pairs of the elements

Basis of relationship state for above mentioned pairs of E/E category elements is shown below [references are made to pages in the following book PebpoB B.E., EpoMOBa O.A. Βητ3μηηβι η MHKpoojieMeHTw, - Μ.: «ΑΛΕΦ-Β», 2003], 1. Calcium/magnesium. Magnesium enhances the absorption of calcium (synergism), the ratio of calcium to the ration of magnesium in the diet should be 2:1. An increased magnesium content can reduce the absorption rate of calcium from the intestinal tract (antagonism), while however severe magnesium deficiency can cause hypocalcaemia. When the amount of magnesium in the blood decreases, kidneys restore balance by keeping less calcium (synergism) (p. 373). 2. Calcium/phosphorus. Phosphorus enhances the absorption of calcium (synergism), the ratio of calcium to the ratio of phosphorus in the diet should be 2:1 (p.373. Calcium excess leads to phosphorus deficiency (antagonism) (p. 557). 3. Iron/Copper. Copper is extremely important for iron uptaking processes (synergism). Excessive consumption and accumulation of iron leads to the deficiency of copper in the body (antagonism) (p. 451). 4. Sodium/potassium. Sodium and potassium are electrolytes that compose all the fluids of the human body participate in a wide range of biochemical reactions. Hypersensitivity to sodium is associated with potassium deficiency in the body (synergism) (p. 479). With an excess of sodium, the level of potassium in the body is reduced due to its enhanced excretion (antagonism). It is known that potassium channels in the cell and hyper polarization of its membrane are physiological means that reduce cellular excitability (p 467). 5. Sodium/magnesium. Magnesium ions are involved in the metabolism of sodium; sodium inhibits the interstitial absorption of magnesium (antagonism) (p. 427). 6. Copper/zinc is one of the most important constants in the human body. Zinc and copper are known physiological antagonists involved in the metallothionein formation. Accumulation or excessive consumption of zinc leads to the deficiency of copper in the body (antagonism) (p. 451). 7. Calcium/potassium. In hypercaliemia treatment first of all it is necessary to administer calcium ions, stimulate the transition of potassium from the extracellular fluid into the cell (antagonism) (p. 363).

All of this makes it necessary to study the balance for each of the above pairs, in particular, the balance of calcium-magnesium, calcium-phosphorus, iron-copper, etc. The norms of normal values are established for the above ratios, generally not coinciding with the possible zones that would be determined by the limits of the normal content of individual elements for each of the above pairs. Therefore, there may be situations, when, for example, the content of both elements of any pair is normal, but the content ratio thereof goes beyond the normal zone for such pair. Previously in such situations was not clear how to restore the balance for such pair, which required the introduction of the concept of concealed alternations such as deficiency (insufficiency) or excess (metabolism disorder) of elements included in one or another pair from the above list.

This statement can be illustrated in the following way. We introduce the designations: jCni is a lower limit of the norm of the element, which is standing in the numerator of the ratio for the pair of the elements; xnu is an upper limit of the norm of the element, which is standing in the numerator of the ratio for the pair of the elements; jtdi is a lower limit of the element, which is standing in the denominator of the ratio for the pair of the elements;

Xdu is an upper limit of the element, which is standing in the denominator of the ratio for the pair of the elements; I/™ = Xnu/xdi is an upper limit of the range for the pair of the elements; ua = Xni/xdu is a lower limit of the range for the pairs of the elements; uu - an upper limit of the reference interval for the ratio of the element content; u\ - a lower limit of the reference interval for the ratio of the element content;

In the standard report on the study of mineral balance, which is presented on the website of the company Trace Elements (http://www.traceelements.com/docs/Graph.pdf, application date 01.06.2016), there are indicated the upper and lower limits of the norm for nine elements of seven above pairs of the essential elements (E/E), which are presented in Table 4.

Table 4

The interval limits of the normal content (reference intervals) for individual elements of seven selected for study pairs of the essential elements

In turn, Table 5 represents the lower and upper limits for the range (built on the basis of Table 4) and predefined limits (received on the said site Trace Elements) for the ratios of above pairs. From this Table it follows that the interval of the normal content (the reference interval) of each of seven selected pairs of the essential elements (E/E) in all cases is already turned out to be of corresponding range. This implies, that for each selected pair of the essential elements there can be a situation, when the content ratio will be beyond the reference interval for this pair, while the content of each individual element from this pair will be normal, that proves the feasibility of the practical use of the proposed method.

Table 5

The limits of the range and the interval of the normal content (reference interval) for seven selected for study pairs of the essential elements

The presence of concealed insufficiency and excess of the content of certain macro-and micronutrients was confirmed laboratorially (by carrying out an analysis of the content of the macro- and micronutrients in other biosubstrates, namely in blood and urine) and clinically (with the results of the correction of the concealed mineral alternations due to the administration of the preparations, intended for the correction of the mineral balance). The clinical data on some patients with the revealed concealed deviations and the results of the treatment thereof for each of the above E/E ratios are shown below. The first 5 examples refer to the study for individual patients and the example 6 refers to the study for a group of patients. The data on the content of the macro- and micronutrients in hair, as well as the ratio of the element content of the selected pair (elements) prior to the beginning of the mineral balance correction for the first five examples is summarized in Table 6.

Table 6

Data on the content of the macro-and micronutrients in hair, as well as the ratio of the element content of the selected element prior to the beginning of the mineral balance correction for the examples Nos. 1-5

Designations: D - deficiency, E - excess, CD - concealed deficiency, CE -concealed excess

Example 1. Calcium/magnesium (insufficiency, hair) - concealed excess of calcium.

It is studied the patient S., 46 years old, with the diagnosis of immunologic thyroiditis, primary hypothyroidism, stage of drug-induced euthyroidism.

She complained about stiffness in the knee joints, cramps of calf muscles, unpleasant sensations during urination. From anamnesis: chronic pyelonephritis, remission stage. The patient has been taking L-thyroxine at a dose of 75 to 100 pg/day for 6 years.

When examined at the time of the study, attention is drawn to the dryness of the skin, especially in the ulnar region, a slight pastosity of the face.

When palpated it is detected that the thyroid gland (TG) is enlarged to the 1 st degree (according to WHO), painless. Regional lymph nodes are not enlarged.

In the TG ultrasound investigation, it is detected that TG is slightly enlarged, total volume 21.5 cm3. The glandular tissue is not uniform due to multiple portions of reduced echogenicity, the contours are not smooth. Portions are from 3 to 7 mm in size, lump in the right lobe is 4.9 mm in size. Conclusion: immunologic thyroiditis, hypertrophic form.

During the electrocardiography it is detected a sinus tachycardia, 88 beats per minute; horizontal position of the electrical cardiac axis (QRS axis); left ventricular hypertrophy.

The results of medical examination: blood hormones were normalized - TSH 2.6 pmlU/L (N 0.4-4.0), T4 11.4 pmol/1 (N 10.3-24.5); antibodies to TPO 293 U/ml (N up to 30); general blood analysis: erythrocytes 5.2xl012/l, leucocytes 6.0xl09/l, in Leukocytic formula - normal; ESR - 11 mm/h; biochemical blood test: cholesterol 5.1 mmol/1 (N up to 5.2), ioduria median was 152.7 pg/l of stable iodine in the urine.

Element content in blood serum (pg/ml): Ca 83.6 (norm, N); Mg 18.2 (N); in hair (pg/g): Ca 1282.2 (N); Mg 108.6 (N). Element content in daily urine (mg/day): Ca 504.1 (N 100-300); Mg 82.4 (N). Thus, the study analysis showed that the concentrations of calcium and magnesium in the blood serum and in hair was within the normal values; in daily urine it was detected an elevated level of calcium in case of normal level of magnesium. In the hair mineralogram, an alteration of the CA/Mg ratio (concealed excess of calcium in hair) was revealed, which was confirmed by an increased content of calcium in daily urine and the clinical picture of the patient.

The patient was diagnosed with urolithiasis, confirmed by the instrumental methods of the investigation.

Example 2. Phosphorus/calcium (excess, hair) - concealed accumulation of phosphorus.

The patient M., bom in 1939, observed from 2011 to 2015 in the scientific centre "MHKP03JIEMEHT" with regard to hypothyroidism in the outcome of TG near-total resection, is taking L-thyroxine of 50 pg/day. In 2014 generalized osteoporosis has been identified according to the densitometry data. In blood tests: PTH is elevated up to 72.4 pg/ml (N 15.0-65.0); 25 OH D3 (transport form of vitamin D) is 14.7 ng/ml; phosphorus is 0.98 mmol/1 (N 0.8-1.55); ionized Ca is 1.22 mmol/1 (N 1.05-1.03); level of Ca in daily urine is 1.38 mmol/1 (N 1.7-5.3), phosphoms in daily urine is 55.5 mmol/day (N 12.9-42). In the parathyroid gland ultrasound investigation of the s (PTG) it is detected echo-signs of volumetric formation from the right upper PTG, size is 0.9x0.4xl.5 cm3. During the scintigraphy: the right upper PTG neoplasm picture.

According to the clinical state of the patient the data of the instrumental and laboratory studies the state is regarded as secondary hyperparathyroidism (confirmed also by hyperphosphaturia and hypocalcemia in daily urine).

Example 3. Iron/copper (excess, hair) - concealed copper deficiency.

The patient, A., 14 years old. Diagnosis: atopic dermatitis, erythematous. Gastroduodenitis, pancreatopathy. Secondary dysbacteriosis of the intestines of II-III degree.

Complaints about weakness; skin rashes; itching; pain in the abdomen, arising after taking acid food, spicy food; flatulence; unstable stool. In the anamnesis - formula feeding, food allergy. Actual exacerbation occurred after eating abundant and fatty foods. In the clinical blood analysis, hypochromic anemia and leukopenia were detected. Biochemical analysis of blood is without pathology. A study of spectral analysis of hair and serum revealed a chromium (0.13 pg/g and 0.067 pg/ml, respectively) and magnesium (54 pg/g and 15.8 pg/ml, respectively) deficiency, in case of the normal copper content level. To confirm the concealed deficiency of copper a daily urine analysis was performed in which a deficiency of copper was detected (10.22 pg/day at N 15.12-50.4). Feces analysis with respect to disbacteriosis revealed a decrease of normal collibacillus up to 14 mln/g, an increase of collibacillus with weakly expressed enzymatic properties (ICS) up to 60%, there was also a decrease in bifidobacteria and enteromycosis.

Complex treatment was carried out which included chromium, 1 tablet of which contains 100 pg of chromium picolinate (NOW, USA) according to the scheme of 200 pg per day for 3 months, the mineral and organic complex 'TeMMoe" (R&amp;D enterprise "Kam>OH", Russia) for 1 capsule once a day in combination with magne B6, 1 tablet of which contains magnesium lactate dihydrate 470 mg (corresponding to 48 mg of magnesium) ("Sanofi-Winthrop Industrie", France) 2 tablets once a day for 3 months.

Biotherapy was performed by combining Bifilysis (5 doses) and Lactobacterin (5 doses) 3 times a day for 1.5 months. Control spectral analysis of hair and daily urine was carried out after 4 months, the stool analysis for dysbacteriosis was measured in a week after the end of the treatment. Clinical improvement occurred at the end of the 1 st week of treatment and characterized by improvement in overall health, a decrease in itching intensity, normalization of the frequency and the stool, a decrease in flatulence and abdominal pains. Clinical signs of intestines dysfunction were stabilized completely on the 30th day from the start of the treatment.

Bacteriologically after the course of treatment the bifidobacteria and lactobacteria content was normalized, the total amount of collibacillus increased to 330 mln/g, the amount of collibacillus with weakly expressed enzymatic properties decreased to 10%, and the elimination of microorganisms with pathogenic potencies (yeast fungi) was detected in the gut organisms.

After the combined treatment, by the end of the second month, atomic dermatitis passed into the stage of remission, skin rashes were completely gone. After 3 months from the beginning of treatment the patient had a persistent recovery (positive dynamics of laboratory and functional indicators - normalization of blood parameters and pancreas size). Weakness stopped. In the mineralogram the copper exchange was restored (based on the clinical picture and the dynamics of the content in daily urine).

Example 4. Sodium/potassium (excess, hair) - concealed accumulation of sodium.

Patient S., 1947, with the diagnosis: immunologic thyroiditis, primary hypothyroidism. She was observed at the Institute of endocrinology for several years. Addressed in March 2014 with complaints about an increased fatigue, tachycardia, a feeling of "lump" in the throat, pastosity of lower limbs. From the anamnesis: during the last 2 years she took L-thyroxine in a dose of 50 pg (monotherapy), thyroid gland was slightly enlarged during the ultrasound investigation of the thyroid gland, signs of diffuse changes were detected in the structure of the gland, as a type of chronic thyroiditis, the hormonal background was not changed, antibodies to TPO 940 (N up to 35 IU/ml, international units per millilitre). During the electrocardiography sinus tachycardia, 90 beats per minute; the normal position of the electrical cardiac axis (QRS axis) were detected. In the analysis the following was detected: a general blood test - leukopenia 2.8xl09/l, a biochemical blood test - cholesterol 6.5 mmol/1. The potassium and sodium content in the serum (pg/ml) and in the hair (pg/g) was within the normal range. In the daily urine an increase in the level of sodium and normal potassium values were revealed.

The patient was under the combined treatment with the mineral-organic complex ’TeMMOc" (R&amp;D enterprise "KaHtoH", Russia) for 1 caps, x 2 times in combination with potassium, 1 tablet of which contains 99 mg of potassium gluconate (RBC, USA) 1 tablet 1 time per day, when treated with L-thyroxin 50 pg for 3 months. The blood test was carried out after 3 months, hair analysis and daily urine for potassium and sodium - after 4 months, a control ultrasound investigation of the thyroid gland 4 months after the start of the treatment.

In dynamics in blood tests was detected the following: leukocytes 4.9x109/l, antibodies to TPO 220 mU/ml (milliunits per milliliter), cholesterol 5.4 mmol/1. During the electrocardiography sinus rhythm was 74 beats per minute.

In the mineralogram the sodium exchange was restored (based on the clinical picture and the dynamics of the content in daily urine).

Improvement of the patient clinical state indicators (the above complaints disappeared), normalization of the data of instrumental and laboratory studies allow to consider the combined treatment effective.

Example 5. Sodium/magnesium (excess, hair) - a concealed deficiency of magnesium.

The patient K. 54 years old, addressed with complaints about emotional lability, general weakness, paresthesia in the upper and lower limbs, increased blood pressure to 140/90 mm Hg, pastosity of the lower limbs, poor sleep, irritability. Objectively: condition is satisfactory, internal organs doesn't have any pathology. In biochemical blood tests - without pathology, a general urine analysis without pathology, daily urine for magnesium content - a deficit of 44.62 mg/day (N 60-210) was detected. In the hair analysis there is a normal content of magnesium and sodium, also concealed deficiency of magnesium is revealed (based on the alteration of the of calcium/magnesium ratio). The correction of the mineral balance of magnesium is prescribed, 1 tablet of which contains 150 mg of magnesium malate (NOW, USA) 1 tablet 1 time per day for 3 months. After the therapy there is a positive dynamic in the clinical state of the patient, the condition stabilized after a month of administration: arterial pressure normalized to 120/80 mm Hg., paresthesias stopped bothering the patient, the mood improved, pastosity has disappeared. After 1.5 months of the administration the vital activity appeared. Stable improvement of the patient clinical state values allows to consider the corrective treatment performed as successful, and the object to reveal the concealed deficiency of the magnet - achieved.

Example 6. Zinc/copper (insufficiency, hair) - concealed zinc deficiency.

In the Scientific and Medical Center "MICROELEMENT", the effect of administration of the preparation "Gemmos" (RPE "Canyon", Russia) on the balance of the macro- and micronutrients of the body was studied. Firstly, we evaluated the effect of "Gemmos" in respect of the balance of the macro- and micronutrients in the blood serum of 15 healthy volunteers on 7th, 14th and 30the day of application [Pycre.viGeKOBci C.A. MnKpoajieMeHTbi npn 3a6ojieBainuix iumtobm^hom >Kejie3Bi -Saarbrucken: LAMBERT Academic Press, 2014. - C. 175], Obtained results are demonstrated in the Table 7.

Table 7

The dynamics of values of the macro-and micronutrients when applying "Gemmos" with healthy volunteers, pg/g/ml (n = 15)

As a result of the study, the reliable decrease of cadmium and lead after 7 and 14 days of administration has been detected. We have observed a more pronounced decrease in these elements in 5.2 and 2.5 times, respectively, after 1 month of "Gemmos" administration. Attention was drawn to the reliable decrease of zinc and "frozen" low values for magnesium in the blood serum after 30 days of correction with "Gemmos" of healthy volunteers; the content of copper, magnesium, calcium reliably did not change. Hair studies regarding copper and zinc content were conducted for all healthy volunteers on the 30th day of "Gemmos" application and all of them had alteration of zinc/copper ratio - concealed zinc deficiency, which was confirmed by analysis of the blood serum.

Suggested method can be performed as follows.

Step 1. Firstly, the content of the macro- and micronutrients in hair or nails is determined with any spectroscopic (mass spectroscopy, atomic absorption, atomic emission, etc.) method.

Step 2. Then, thus determined value of the content is compared with the limits of a normal content interval for every macro- or micronutrient, subsequently judgement is made whether deviation with respect to the norm (towards excess or deficiency) was detected for this element. Wherein for the essential and conditionally essential elements both upper and lower limits of the normal content interval are determined, while for the conditionally toxic elements only the upper limit is detennined, since the lower limit of the normal content, also known as the lower limit of the guaranteed normal content interval, coincides with zero.

Fig. 1 represents an explanation for the comparison procedure of the measurement results with the limits of the normal content interval for the essential and conditionally essential elements, the conditionally toxic elements are shown in fig. 2, and explanations for graphs on figs. 1 and 2, respectively, are given in Table 8 and Table 9.

Table 8

Explanations to the graphs of deviations of the essential and conditionally essential elements

Table 9

Explanations to the graphs of deviations of the conditionally toxic elements

On these drawings and in these tables for single elements the following designations are used: x - measured absolute value of the element content, gg/g; = (xu - xi)/2 - mean value (middle point) of the reference interval (RI), wherein xu -upper limit of the reference interval, and x\ - lower limit of the reference interval; / - half-width of the reference interval (for the conditionally toxic elements the lower limit of the reference interval coincides with 0, as opposed to essential and conditionally essential elements, for which this is not observed); y = (x - xm)// - normalized (to the half-width of the reference interval) centered (with respect to the middle of the reference interval) value of the element content, also referred to as (normalized) deviation (with respect to the middle of the reference interval); L - biologically admissible level; a - zone width of the upper limit of the norm (ULN), and in case of the essential and conditionally essential elements - also the lower limit of the norm (LLN). d - "extreme" value (how far upper and lower limits of the norm go beyond the limits of the reference interval; the amount of this value can be set based on the analysis of measurement error, percentage units or put equal to zero). x/xu - degree of extending beyond the upper limit of the reference interval for element content, referred to as "above ULN, times"; xilx - degree to which the content of the element is lower than the lower limit of the reference interval, referred to as "below LLN, times" (for the conditionally toxic elements this value equals zero).

Step 3. After the decryption of the data of each individual element, determination of the concealed deviations for the elements from selected pairs is performed. A ratio of the content for the selected pair of elements (essential, as well as essential and conditionally toxic) allows for determination of concealed disorders: • the ratio of two essential elements concealed deficiency and concealed excess of one of the two elements in a pair; • the ratio of the conditionally toxic and essential elements - concealed excess of the conditionally toxic element in a pair.

Below for simplicity of examination of the case of the concealed disorders the following abbreviations are used: N - norm; D - denominator; N - numerator; NN - norm for an element in the numerator; ND - norm for an element in the denominator; DULN - distance to the upper limit for an element in the numerator; DLLD - distance to the lower limit for an element in the denominator; CEN - concealed excess for an element in the numerator; CDD - concealed deficiency for an element in the denominator; CDN - concealed deficiency for an element in the numerator; CED - concealed excess for an element in the denominator; E/E - content ratio for two essential elements (dimensionless); CT/E - content ratio for conditionally essential and essential elements (dimensionless).

Designations for the content ratios of the elements in the selected pair of elements: xn - content of the element, standing in the numerator of the ratio for the pair of the elements; Χά - content of the element, standing in the denominator of the ratio for the pair of the elements; u = Xn/xd - content ratio of the elements (such ratio is correct since lower limit of the reference interval for the essential elements is always above zero); uu - upper limit of the reference interval for the content ratio of the elements; in - lower limit of the reference interval for the content ratio of the elements; / = (mu - u\)!2 - half-width of the reference interval for the ratio of elements (for the ratio of the conditionally toxic and essential elements lower limit of the reference interval u\ = 0);

Um = (mu + u\)/2 - average value (middle point) of the reference interval; v = (u - Mm)// - normalized (to the half-width of the reference interval) centered (with respect to the middle of the reference interval) value of the content ratio of the elements, also referred to as (normalized) deviation (with respect to the middle of the reference interval); cimi - width of the zone of the upper and lower limit of the norm for the ratio (for the ratio of the conditionally toxic and essential elements there is no zone of the lower limit of the norm); dm - "extreme" value for the ratio (how far upper and lower limits of the norm go beyond the limits of the reference interval; the amount of this value can be set based on the analysis of measurement error, percentage units or put equal to zero); u/uu - degree of extending beyond the upper limit of the reference interval for the content ratio of the elements, referred to as "above ULN, times"; u\/u - degree to which the content ratio of the elements is lower than the lower limit of the reference interval, referred to as "below LLN, times" (for the ratio of the conditionally toxic and essential elements this value equals zero).

Herein indexes are the same as for the single elements.

Thus, for each pair based on the results of the analysis of the single elements in a pair the following input data is available: 1. Absolute content values for each pair of the elements, the content of one of which is in the numerator, and of another one is in the denominator, more specifically: N (.i'n) and D (χχι). 2. Normalized centered content values for each pair of the elements numerator (yn) and denominator (yd). 3. 6 numbers - data regarding the ratio of the numerator and denominator with the norm, formed during the decryption of the content study results for the individual elements from a pair, more specifically: N (xn) = NN, N > NN, N < NN, D (xd)= ND, D > ND, D < ND (in case of the conditionally toxic elements, which stand in the numerator, ratio N < NN cannot exist).

In the tables below all possible cases of the element ratios in the selected pair are listed (Table 10 - essential elements, Table 11 conditionally toxic and essential elements). Dash in the last column corresponds to the absence of the concealed deviations.

Table 10

Possible ratios between element contents in the selected pair and corresponding concealed deviations (in case of the pair of essential elements)

Table 11

Possible ratios between element contents in the selected pair and corresponding concealed deviations (the content of the conditionally toxic element in numerator, and of the essential - in the denominator)

Obtained results of the calculations (decryption) both for individual elements and for pairs of the elements are presented in the form of the table "Problematic elements (deviations from the norm)". Table 12 corresponding to the above-mentioned Example 1 is provided below.

Table 12

Problematic elements (deviations from the norm)

Designations: LLN - lower limit of the norm; ULN - upper limit of the norm; SC -substantial content; RI reference interval; BAL biologically admissible level.

The detection results of the concealed disorders are not entered into this table but are used during formation of conclusion regarding conducted studies. Below is given the conclusion of the mineralogram, made based on Table 12.

The conclusion of the mineralogram 1. Syndrome of the molybdenum deficiency 2. Chrome deficiency 3. Mild sulphur deficiency and selenium deficiency 4. Copper deficiency 5. Disorder of potassium metabolism 6. Concealed disorder of calcium metabolism (see disorder in the pair Calcium/Magnesium below) 7. Upper limit of the norm for titanium content - biologically admissible level (BAL) 8. Alteration of the sodium/potassium ratio (decreasingly) 9. Alteration of the calcium/magnesium ratio (increasingly)

Step 4. When relative to any element a fact of the metabolism disorder in a clear or concealed form is established, this on the basis of the previously detected correlation allows for making a diagnosis with high reliability and detecting one or another disease.

Tables 8 and 9 define an order of making a judgement regarding the presence or absence of concealed disorders for individual elements determined based on the analysis of the content ratio for a pair of the elements and individually for each element from the pair. Below are given rules for making such judgements separately for essential elements and ratios of conditionally toxic and essential elements. In operations listed below centered (with respect to the middle point of the reference interval) and normalized (to the half-width of the reference interval) values of the contents and distances are indicated. 1. Content ratios of the essential elements: - assume that a content ratio of the pair of the essential elements is above the norm and a content of the elements in the numerator and denominator is in the norm; provided that the distance from the upper limit of the norm for the element in the numerator to the content value of this element is less than the distance from the lower limit of the norm for the element in the denominator to the content value of this element, then the judgement is made about the concealed excess of the element in the numerator, otherwise about concealed deficiency of the element in the denominator; - assume that a content ratio of the pair of the essential elements is above the norm, a content of the element in the numerator is in the norm, a content of the element in the denominator is above the norm; then the judgement is made about the concealed deficiency of the element in the denominator; - assume that a content ratio of the pair of the essential elements is above the norm, a content of the element in the numerator is below the norm, a content of the element in the denominator is in the norm; then the judgement is made about concealed deficiency of the element in the denominator; - assume that a content ratio of the pair of the essential elements is below the norm, a content of the elements in the numerator and denominator is in the norm; provided that distance from the upper limit of the norm for the element in the numerator to the content value of this element is greater than the distance from the lower limit of the norm for the element in the denominator to the content value of this element, then the judgement is made about concealed deficiency of the element in the numerator, otherwise about concealed excess of the element in the denominator; - assume that a content ratio of the pair of the essential elements is below the norm, a content of the element in the numerator is in the norm, a content of the element in the denominator is below the norm; then the judgement is made about concealed deficiency of the element in the numerator; - assume that a content ratio of the pair of the essential elements is below the norm, a content of the element in the numerator is above the norm, a content of the element in the denominator is in the norm; then the judgement is made about concealed excess of the element in the denominator; - in all other cases, it is assumed that there are no concealed disorders. 2. The content ratio of the conditionally toxic and essential elements - assume that a content ratio of the conditionally toxic and essential elements is above the norm, a content of the conditionally toxic element (in the numerator) is in the norm; provided that content of the conditionally essential element (in the denominator) is in the norm or above the norm, then judgement is made about concealed excess of the conditionally toxic element (in the numerator); - in all other cases, it is assumed that there are no concealed disorders. Data regarding application of the invention

This method has been already successfully used in the Scientific and Medical Center "MICROELEMENT" for a few years, with its help the calculations have been done for the thousands of the patients. Over the years its effectiveness and the reliability of the results obtained with its help have been proved.

The system for implementation of the method provided, being the spectrometric system for studying the mineralogram of the body based on hair or nail analysis, operates as follows.

In the input device 1 a biosubstrate to be studied (advantageously samples of hair or nails) is introduced, and in this device, the preparation of the biosubstrate is carried out. Spectrometric study is performed in the evacuated part 18 of the system, which comprises an ion source 2, a mass analyzer 3, a detector 4 and a pumping device 7, connected as shown on the figure. Pumping device 7 performs the evacuation, i.e., evacuation of the air and gases for the creation of the depression (vacuum). In the ion source 2 studied biosubstrate is partially ionized and formation of the ion beam occurs, which enters the input of the mass analyzer 3 through the ion beam transmission channel 21. In the mass analyzer 3 separation of the ions based on the ratio value of the ion mass m and its charge e occurs, the mass separated ionsenter trough the input of the detector 4 through the feed channel 22 for the ions separated by mass, and information regarding the mass of the ions through the line 23 of sending ion mass data enters through one of the inputs of the first computer 6. In the detector 4 the ion current is converted to the electric current, which then is amplified and through the line 24 of sending a signal with ion current data enters through the input to the data processing unit 5, where this information is registered. Data from the output of the unit 5 through the line 25 of sending ion current data enters through the second input of the first computer 6.

Therefore, information about the ion mass (through the line) and about ion current (through the line) enters into the first computer 6, which allows for determining which elements are comprised in the studied biosubstrate and what is the content of each element. This information is presented in the machine-readable form and as a digital signal x containing information about the content of each element, is supplied for the following calculation through the line 26 of sending a signal about the measured absolute content value for individual elements into two parallel channels - channel 19 for detennination of the distinct deviations and channel 20 for determination of concealed deviations. Wherein the channel 19 for determination of distinct deviations contains a centering and normalization unit 8 for individual elements, a reference interval parameter setting unit 11 for individual elements and a decryption unit 13 for individual elements, and the channel 20 for determination of the concealed deviations contains a unit 9 for forming ratios of the element contents in a pair, a centering and normalization unit 10 for pair ratios, a reference interval parameter setting unit 12 for pair ratios, a unit 14 for calculating and comparing the distance to upper and lower limits, concealed deviation detection units 15 and a pair ratio decryption unit 16.

Signal x through the line 26 enters one input of the unit of the channel for determination of the distinct deviation, on the other input of which enters the data xi, xu, xm through the line 27 of setting reference interval parameter for individual elements from the unit 11. In the unit 8, signal x, characterizing the content of a certain element, is compared with parameters of the reference interval for this element and formation of the signal y = (x xcp)//, which represents the deviation of the measured content of this element from the middle of the reference interval, normalized to the half-width of this interval, is carried out.

For the operation description of the channel 20 there is a need in the following designations for the content ratios of the elements in the selected pair of the elements:

Xn - content of the element standing in the numerator of the ratio for the pair of the elements;

Xd - content of the element standing in the denominator of the ratio for the pair of the elements; u = xn/xd content ratio of the elements (such ratio is correct since lower limit of the reference interval for the essential elements is always above zero); uu - upper limit of the reference interval for the content ratio of the elements; m - lower limit of the reference interval for the content ratio of the elements; / = (Uu - m)/2 - half-width of the reference interval for the ratio of elements (for the ratio of the conditionally toxic and essential elements lower limit of the reference interval mi = 0);

Um = («u + in) 12 - average value (middle point) of the reference interval; v = (u - Mm)// normalized (to the half-width of the reference interval) centered (with respect to the middle of the reference interval) value of the content ratio of the elements, also referred to as (normalized) deviation (with respect to the middle of the reference interval); «rat - width of the zone of the upper and lower limit of the norm (for the ratio of the conditionally toxic and essential elements there is no zone of the lower limit of the norm); dm\ - "extreme" value (how far upper and lower limits of the norm go beyond the limits of the reference interval; the amount of this value can be set based on the analysis of measurement error, e.g., percentage units or put equal to zero).

Signal y through the line 28 of sending a deviation signal for individual elements enters through the input of the second computer 17 and through the inputs of the decryption unit 13 for individual elements and the unit 14 for calculating and comparing the distance to upper and lower limits. In decryption unit 13 for individual elements based on the signal y the decision is taken with respect to that in which of the zones content value of this element falls - in the zone below the lower limit of the norm (LLN), in the zone LLN, the zone of the guaranteed normal content, the zone of the upper limit of the norm (ULN) or the zone above ULN (1 from 5 options -respectively "Deficiency", "LLN", "Norm", "ULN", "Excess"). Information about this is received by the input of the second computer 17 through the line 29 of sending information about the entering zone for individual elements.

Signal x through the line 26 also is received by the input of the unit 9 for forming ratios of the element contents in a pair of the determination channel 20 of the concealed deviations, which selects from all elements for the following use only those, which were determined for formation of the ratios in the pair (advantageously essential/essential and conditionally toxic/essential elements). In the output of the unit 9 for forming ratios of the element contents in a pair signal u is produced, the said signal is indicative of the ratio of the element content in the pair, and this signal through the line 30 of sending a signal of the content ratio of elements in a pair is transferred to the first input of the centering and normalization unit 10 for pair ratios, on the second input of which through the line 31 of setting reference interval parameter for a pair the data u\, uu, um are received. In aforementioned unit 10 the signal u, which is indicative of the ratio of the element content of the pair, is compared to the parameters of the reference interval for this pair and it is provided a formation of the signal v, which represents a deviation of the ratio of the element content of the pair from the reference interval middle, normalized by the half-width of this interval. Signal v through the line 32 of sending deviation signal for a pair is received by the input of second computer 17 and by the input of pair ratio decryption unit 16, in which the decision is taken with respect to that in which of the zones the content ratio of the elements of the pair falls - in the zone below the lower limit of the norm (LLN), in the zone LLN, the zone of the guaranteed normal content, the zone of the upper limit of the norm (ULN) or the zone above ULN (1 from 5 options - respectively "Deficiency", "LLN", "Norm", "ULN", "Excess", as in case of the individual elements). Information about such decision through the line 33 of sending information about the entering zone for a pair is received by the first input of the concealed deviation detection unit 15, two other inputs of which from the determination channel 19 of the distinct deviations receive the signals through line 34 of sending data, which are indicative of the proximity to the limits (1 from 2 options - "Yes” (DULN > DLLD), "No" (DULN < DLLD) from the output of the unit 14 and a signal through the line 29 from the output of the unit 13.

In this case, it is about the pairs of the elements, the content ratios for which are characterized by the deviation from the norm, which was identified in the pair ratio decryption unit 16. In the concealed deviation detection unit 15 based on the information, received by its three inputs the decision is taken with respect to the presence or absence of the concealed deviation for one of the two elements of the pair, in this regard a corresponding signal (1 from 5 options the absence of the concealed deviation, the concealed excess of the numerator (CEN), the concealed deficiency of the numerator (CDN), the concealed excess of the denominator (CED), the concealed deficiency of the denominator (CDD)) is transmitted through the line 35 of sending data about the presence of the concealed deviations on the third input of the second computer 17. Making a decision about absence or presence of the corresponding concealed deviations is carried out in the unit 15 based on the Tables 10 and 11 above, where all possible cases of the element ratios in the selected pair are listed. Dash in the last column of the tables corresponds to the absence of the concealed deviations.

Table 4

Possible ratios between element contents in the selected pair and corresponding concealed deviations (the content of the conditionally toxic element - in numerator, and of the essential element - in the denominator)

Thus, the necessary technical result in the suggested system is achieved, consisting in increasing the accuracy and sensitivity of the system by expanding its functional capabilities, so as to provide capabilities based on the measurement results to create mineralogram of the body and determine, the content of which chemical elements go beyond the normal values in distinct or concealed form for the further application of this information in order to diagnose diseases.

Claims (5)

1. Claims

   1. A method for determining the balance of the macro- and micronutrients in the body, which consists in determination of a content of the macro- and micronutrients in a biosubstrate, hair and nails of the patient being advantageously used as the biosubstrate, wherein the content of the macro- and micronutrients and correlations thereof are defined, and the changes with respect to the norm of the content of the macro- and micronutrients are registered based on the values of the absolute deviations from the norm, and are used for referring the patient to the risk group with associated disease, wherein the changes with respect to the norm of the content of the macro- and micronutrients are further determined based on the values of the relative deviations from the norm at ratios of pairs of the macro- and micronutrients, wherein the acceptable intervals for the absolute deviations from the norm of the content for the macro- and micronutrients and the acceptable intervals for the relative deviations from the norm at ratios of pairs of the macro- and micronutrients are predetermined, and the patient is referred to the risk group with associated disease based on the data of going beyond the acceptable intervals of the absolute deviations of the macro- and micronutrients and/or data of going beyond the acceptable intervals of the relative deviations from the norm at ratios of pairs of the macro- and micronutrients, and/or based on the concealed deviation of one element from the pair beyond the acceptable intervals at ratios of pairs of the macro- and micronutrients.
2. 2. The method of claim 1, wherein the macro- and micronutrients, the content of which is determined in a biosubstrate are the essential macronutrients Ca, Cl, F, K, Na, Mg, P and S and the essential micronutrients Cr, Cu, Fe, 1, Mn, Mo, Se, Zn, as well as the conditionally essential micronutrients Ag, Al, Au, B, Br, Co, Ge, Li, Ni, Si, V and the conditionally toxic micronutrients As, Ba, Be, Bi, Cd, Ce, Cs, Dy, Er, Eu, Ga, Gd, Hf, Hg, Ho, In, Ir, La, Lu, Nb, Nd, Os, Pb, Pd, Pr, Pt, Rb, Re, Rh, Ru, Sb, Sc, Sm, Sn, Sr, Ta, Tb, Te, Th, Ti, Tl, Tm, U, W, Y, Yb, Zr are used.
3. 3. The method of claim 1, wherein the ratios of pairs of the essential macro- and micronutrients are Ca/P, Na/K, Na/Mg, Ca/Mg, Fe/Cu, Zn/Cu, Ca/K, and of the conditionally toxic and essential elements are Pb/S, Pb/Fe, Pb/Ca, Cd/Zn, Cd/S, Hg/Z, Hg/Se, Hg/S, Hg/Zn.
4. 4. The method of claim 1, wherein the normal content interval for the essential and conditionally essential macro- and micronutrients includes a zone of the lower limit of the interval, a zone of the guaranteed normal content and a zone of the upper limit of the interval, and for the conditionally toxic micro elements - a zone of the guaranteed normal content and a zone of the upper limit of the interval.
5. 5. A system for implementation of the method of claim 1, comprising a biosubstrate input device, a data processing unit, a first computer, an ion source, the input of which is connected to the biosubstrate input device output, a mass analyzer, the input of which is connected to the ion source output, a detector, the input of which is connected to the first output of the mass analyzer, the first output is connected to the input of the data processing unit, the output of which is connected to the first input of the first computer, the second input of which is connected to the second output of the mass analyzer, as well as a pumping device, the first, second and third inputs of which are connected to the second outputs of the ion source and detector and to the third output of the mass analyzer, wherein a reference interval parameter setting unit for individual elements, a centering and normalization unit for individual elements, the first input of which is connected to the output of the reference interval parameter setting unit for individual elements, and the second input is connected to the output of the first computer, a second computer, a decryption unit for individual elements, the input of which is connected to the output of the centering and normalizing unit for individual elements, and the output is connected to the first input of the second computer, a unit for calculating and comparing the distance to the upper and lower limits, the input of which is connected to the output of the centering and normalization unit for individual elements and to the second input of the second computer, a unit for forming the ratios of the contents of the elements in a pair, the input of which is connected to the first computer output, a reference interval parameter setting unit for the pair ratios, a centering and normalization unit for the pair ratios, the first input is connected to the output of the reference interval parameter setting unit for the pair ratios, and the second input is connected to the output of the unit for forming the ratios of the contents of the elements in a pair, a pair ratio decryption unit, the input of which is connected to the output of the centering and normalization unit for the pair ratios and to the third input of the second computer, and a concealed deviation detection unit, the first input of which is connected to the pair ratio decryption unit, the second input is connected to the output of the unit for calculating and comparing the distance to the upper and lower limits, the third input is connected to the output of the decryption unit for individual elements, and the output is connected to the fourth input of the second computer.