AU2015201984A1 - SUBSTITUTED PYRAZOLO[1,5-a]PYRIMIDINE COMPOUNDS AS TRK KINASE INHIBITORS - Google Patents

Abstract

[00655] Compounds of Formula (I) and salts thereof in which R1, R2 , R3, R4 , X, Y and n have the meanings given in the specification, are inhibitors of Trk kinases and are useful in the treatment of diseases which can be treated with a Trk kinase inhibitor such as pain, cancer, inflammation, neurodegenerative diseases and certain infectious diseases.

Description

WO 2011/006074 PCT/US2010/041538 1 SUBSTITUTED PYRAZOLO[1,5-a]PYRIMIDINE COMPOUNDS AS TRK KINASE INHIBITORS [0001] The present invention relates to novel compounds, to pharmaceutical compositions comprising the compounds, to processes for making the compounds and to the use of the compounds in therapy. More particularly, it relates to certain substituted pyrazolo[1,5-a]pyrimidine compounds which exhibit Trk family protein tyrosine kinase inhibition, and which are useful in the treatment of pain, cancer, inflammation, neurodegenerative diseases and certain infectious diseases. [0002] The current treatment regimes for pain conditions utilize several classes of compounds. The opioids (such as morphine) have several drawbacks including emetic, constipatory and negative respiratory effects, as well as the potential for addictions. Non steroidal anti-inflammatory analgesics (NSAIDs, such as COX-1 or COX-2 types) also have drawbacks including insufficient efficacy in treating severe pain. In addition, COX-1 inhibitors can cause ulcers of the mucosa. Accordingly, there is a continuing need for new and more effective treatments for the relief of pain, especially chronic pain. [0003] Trk's are the high affinity receptor tyrosine kinases activated by a group of soluble growth factors called neurotrophins (NT). The Trk receptor family has three members: TrkA, TrkB and TrkC. Among the neurotrophins are (i) nerve growth factor (NGF) which activates TrkA, (ii) brain-derived neurotrophic factor (BDNF) and NT-4/5 which activate TrkB and (iii) NT3 which activates TrkC. Trk's are widely expressed in neuronal tissue and are implicated in the maintenance, signaling and survival of neuronal cells (Patapoutian, A. et al., Current Opinion in Neurobiology, 2001, 11, 272-280). [0004] Inhibitors of the Trk/neurotrophin pathway have been demonstrated to be effective in numerous pre-clinical animal models of pain. For example, antagonistic NGF and TrkA antibodies such as RN-624 have been shown to be efficacious in inflammatory and neuropathic pain animal models (Woolf, C.J. et al. (1994) Neuroscience 62,327-331; Zahn, P.K. et al. (2004) J. Pain 5, 157-163; McMahon, S. B. et al., (1995) Nat. Med. 1, 774-780; Ma, Q. P. and Woolf, C. J. (1997) Neuroreport 8, 807-810; Shelton, D. L. et al. (2005) Pain 116, 8-16; Delafoy, L. et al. (2003) Pain 105, 489-497; Lamb, K. et al. (2003) Neurogastroenterol. Motil. 15, 355-361; Jaggar, S. I. et al. (1999) Br. J. Anaesth. 83, 442 448) and neuropathic pain animal models (Ramer, M. S. and Bisby, M. A. (1999) Eur. J. Neurosci. 11, 837-846; Ro, L. S. et al. (1999); Pain 79, 265-274 Herzberg, U. et al. (1997) Neuroreport 8, 1613-1618; Theodosiou, M. et al. (1999) Pain 81, 245-255; Li, L. et al.

WO 2011/006074 PCT/US2010/041538 2 (2003) Mol. Cell. Neurosci. 23, 232-250; Gwak, Y. S. et al. (2003) Neurosci. Lett. 336, 117 120). Additionally, recent literature indicates after inflammation, BDNF levels and TrkB signaling is increased in the dorsal root ganglion (Cho, L. et al. Brain Research 1997, 749, 358) and several studies have show antibodies that decrease signaling through the BDNF/TrkB pathway inhibit neuronal hypersensitization and the associated pain (Chang-Qi, L et al. Molecular Pain 2008, 4:27). [00051 It has also been shown that NGF secreted by tumor cells and tumor invading macrophages directly stimulates TrkA located on peripheral pain fibers. Using various tumor models in both mice and rats, it was demonstrated that neutralizing NGF with a monoclonal antibody inhibits cancer related pain to a degree similar or superior to the highest tolerated dose of morphine. In addition, activation of the BDNF/TrkB pathway has been implicated in numerous studies as a modulator of various types of pain including inflammatory pain (Matayoshi, S., J. Physiol. 2005, 569:685-95), neuropathic pain (Thompson, S.W., Proc. Natl. Acad. Sci. USA 1999, 96:7714-18) and surgical pain (Li, C.-Q. et al., Molecular Pain, 2008, 4(28), 1-11). Because TrkA and TrkB kinases may serve as a mediator of NGF driven biological responses, inhibitors of TrkA and/or other Trk kinases may provide an effective treatment for chronic pain states. [0006] Recent literature has also shown that overexpression, activation, amplification and/or mutation of Trk kinases are associated with many cancers including neuroblastoma (Brodeur, G. M., Nat. Rev. Cancer 2003, 3, 203-216), ovarian (Davidson. B., et al., Clin. Cancer Res. 2003, 9, 2248-2259) and colorectal cancer (Bardelli, A., Science 2003, 300, 949). In preclinical models of cancer, non-selective small molecule inhibitors of Trk A, B and C were efficacious in both inhibiting tumor growth and stopping tumor metastasis (Nakagawara, A. (2001) Cancer Letters 169:107-114; Meyer, J. et al. (2007) Leukemia, 1 10; Pierottia, M.A. and Greco A., (2006) Cancer Letters 232:90-98; Eric Adriaenssens, E. et al. Cancer Res (2008) 68:(2) 346-35 1). [00071 In addition, inhibition of the neurotrophin/Trk pathway has been shown to be effective in treatment of pre-clinical models of inflammatory diseases with NGF antibodies or non-selective small molecule inhibitors of Trk A, B and C. For example, inhibition of the neurotrophin/Trk pathway has been implicated in preclinical models of inflammatory lung diseases including asthma (Freund-Michel, V; Frossard, N.; Pharmacology & Therapeutics (2008), 117(1), 52-76), interstitial cystitis (Hu Vivian Y; et. al. The Journal of Urology (2005), 173(3), 1016-21), inflammatory bowel diseases including ulcerative colitis and WO 2011/006074 PCT/US2010/041538 3 Crohn's disease (Di Mola, F. F, et. al., Gut (2000), 46(5), 670-678) and inflammatory skin diseases such as atopic dermatitis (Dou, Y.-C.; et. al. Archives of Dermatological Research (2006), 298(1), 31-37), eczema and psoriasis (Raychaudhuri, S. P., et al., J. Investigative Dermatology (2004), 122(3), 812-819). [0008] The neurotrophin/Trk pathway, particularly BDNF/TrkB, has also been implicated in the etiology of neurodegenerative diseases including multiple sclerosis, Parkinson's disease and Alzheimer's Disease (Sohrabji, Farida; Lewis, Danielle K., Frontiers in Neuroendocrinology (2006), 27(4), 404-414). [0009] The TrkA receptor is also thought to be critical to the disease process in the infection of the parasitic infection of Trypanosoma cruzi (Chagas disease) in human hosts (de Melo-Jorge, M. et al. Cell Host & Microbe (2007), 1(4), 251-261). [0010] Trk inhibitors may also find use in treating disease related to an imbalance of the regulation of bone remodeling, such as osteoporosis, rheumatoid arthritis, and bone metastases. Bone metastases are a frequent complication of cancer, occurring in up to 70 percent of patients with advanced breast or prostate cancer and in approximately 15 to 30 percent of patients with carcinoma of the lung, colon, stomach, bladder, uterus, rectum, thyroid, or kidney. Osteolytic metastases can cause severe pain, pathologic fractures, life threatening hypercalcemia, spinal cord compression, and other nerve-compression syndromes. For these reasons, bone metastasis is a serious and costly complication of cancer. Therefore, agents that can induce apoptosis of proliferating osteoblasts would be highly advantageous. Expression of TrkA and TrkC receptors has been observed in the bone forming area in mouse models of bone fracture (K. Asaumi, et al., Bone (2000) 26(6) 625 633). In addition, localization of NGF was observed in almost all bone forming cells (K. Asaumi, et al.). Recently, it was demonstrated that a pan-Trk inhibitor inhibits the tyrosine signaling activated by neurotrophins binding to all three of the Trk receptors in human hFOB osteoblasts (J. Pinski, et al., (2002) 62, 986-989). These data support the rationale for the use of Trk inhibitors for the treatment of bone remodeling diseases, such as bone metastases in cancer patients. [0011] Several classes of small molecule inhibitors of Trk kinases said to be useful for treating pain or cancer are known (Expert Opin. Ther. Patents (2009) 19(3), 305-319). [0012] Pyrazolo[1,5-a]pyrimidine compounds are known. For example, International patent application publication WO 2004/089415 discloses certain pyrazolo[1,5-a]pyrimidine 3-carboxamide compounds having a phenyl, thienyl or furyl group in the 5-position which WO 2011/006074 PCT/US2010/041538 4 are said to be 11 -beta-hydroxysteroid dehydrogenase type 1 inhibitors useful in combination therapies. [00131 European patent application publication No. EP 1948633A2 describes 5 phenyl-7-hydroxy-substituted pyrazolo[1,5-a]pyrimidine-3-carboxamide compounds as casein kinase II modulators for treating cancer. [0014] PCT publication WO 2010/051549 describes pyrazolopyrimidine compounds having the general structure: H R R 1 0 [00151 said to be inhibitors of Jak kinases. [0016] It has now been found that certain pyrazolo[1,5-a]pyrimidine compounds bearing an aryl-substituted or heteroaryl-substituted heterocyclic group at the 5-position and a group having the formula C(=O)NR R2 at the 3-position, wherein R and R 2 are as defined herein, are inhibitors of Trk kinases, in particular inhibitors of TrkA and/or TrkB and/or TrkC, and are useful for treating disorders and diseases such as cancer and pain, including chronic and acute pain. Certain compounds which are inhibitors of TrkA and/or TrkB may be useful in the treatment of multiple types of pain including inflammatory pain, neuropathic pain, and pain associated with cancer, surgery, and bone fracture. In addition, compounds of the invention may be useful for treating cancer, inflammation, neurodegenerative diseases and certain infectious diseases. [00171 In addition, compounds of the invention have been shown to be selective for the Trk family of kinases over closely related kinases. In particular, compounds of the invention are more selective for inhibiting TrkA kinase activity over inhibiting the activity of one or more members of the Jak kinase family (Jakl, Jak2, Jak3 and Tyk2). Inhibition of the Jak family of kinases has been postulated or demonstrated to result in several unwanted side effects including CD8 T and NK cell depletion (which can result in loss of tumor surveillance and increased infections), elevated cholesterol, neutropenia, thrombocytopenia, decreased reticulocytes (resulting in anemia) and bone marrow suppression (Igaz P. et al., Inflamm. Res., 2001, 50:435-441; O'Shea J.J., Immunity, 1997, 7:1-11; Ihle J.N. et al., Canc. J. Sci. Am., 1998, 4 suppl 1 S84-91; Gupta P. et al., J. Clin. Pharm. 2009; Kremer J.M. et al., Arth. & Rheum., 2009, 60:1895-1905 and van Gurp E., et al., Am. J. Transpl, 2008, 8:1711-18).

WO 2011/006074 PCT/US2010/041538 5 Accordingly, compounds of the invention may be more suitable as therapeutic treatments owing to their ability to inhibit the Trk family of kinases in preference over closely related kinases such as the Jak family of kinases, and therefore may avoid unwanted side effects in a mammal being treated with a compound of the invention. [00181 Accordingly, one embodiment of this invention provides a compound of the general Formula I: R 3 N >LN N R1 (R 4)n N x O R2 [0019] or a salt thereof, wherein: [0020] R 1 is H or (1-6C alkyl); [0021] R 2 is H, (1-6C)alkyl, -(1-6C)fluoroalkyl, -(1-6C)difluoroalkyl, -(1 6C)trifluoroalkyl, -(1-6C)chloroalkyl, -(2-6C)chlorofluoroalkyl, -(2-6C)difluorochloroalkyl, -(2-6C)chlorohydroxyalkyl, -(1-6C)hydroxyalkyl, -(2-6C)dihydroxyalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2

NH

2 , -(1-6C alkyl)NHSO 2 (1-3C alkyl), -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl), -(1-6C alkyl)N(1-4C alkyl) 2 , -(1-6C alkyl)NHC(=0)O(1-4C alkyl), (1-6C alkyl)hetCyc', -(1-6C alkyl)hetArl, hetAr 2 , hetCyc 2 , -O(1-6C alkyl) which is optionally substituted with halogen, OH or (1-4C)alkoxy, -O(3-6C cycloalkyl), Cyc', -(1-6C alkyl)(3-6C cycloalkyl), -(1-6Calkyl)(1-4C alkoxy), -(1-6C hydroxyalkyl)(1-4C alkoxy), a bridged 7-membered cycloalkyl ring optionally substituted with (1-6C)hydroxyalkyl, or a bridged 7-8 membered heterocyclic ring having 1-2 ring nitrogen atoms; [0022] or NR 1

R

2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from (1-6C)alkyl, OH, CO 2 H, (1-3C alkyl)CO 2 H, -0(1-6C alkyl) and (1-6C)hydroxyalkyl; [00231 hetCyc' is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc' is optionally substituted with oxo, OH, halogen or (1-6C)alkyl; [0024] hetCyc 2 is a 6 membered carbon-linked heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc 2 is optionally substituted with F, SO 2

NH

2 , SO 2 (1-3C alkyl) or halogen; [00251 hetArl is a 5-membered heteroaryl ring having 1-2 ring heteroatoms independently selected from N and 0 and optionally substituted with (1-4C)alkyl; WO 2011/006074 PCT/US2010/041538 6 [00261 hetAr 2 is a 5-6 membered heteroaryl ring having 1-2 ring nitrogen atoms and optionally substituted with one or more substituents independently selected from (1-4C)alkyl, (3-6C)cycloalkyl, halogen and OH; [00271 Cyc' is a 3-6 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H, (1-4C alkyl)OH, halogen and CF 3 ; [00281 Y is (i) phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 , -(1 4C alkyl)hetCyc 3 , -O(1-4C alkyl)O(1-3C alkyl) and -O(3-6C dihydroxyalkyl), or (ii) a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl), (1-4C)alkyl and NH 2 , or (iii) a pyrid-2-on-3-yl ring optionally substituted with one or more substituents independently selected from halogen and (1 4C)alkyl; [0029] hetCyc 3 is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0 and optionally substituted with (1-6C)alkyl; [00301 X is null, -CH 2 -, -CH 2

CH

2 -, -CH 2 0- or -CH 2 NRd-; [00311 Rd is H or -(1-4C alkyl); [0032] R 3 is H or -(1-4C alkyl); [00331 each R4 is independently selected from halogen, -(1-4C)alkyl, -OH, -(1 4C)alkoxy, -NH 2 , -NH(1-4C alkyl) and -CH 2 OH; and [0034] nis0, 1,2,3,4,5 or6. [00351 In one embodiment of Formula I, X is selected from any of the values described above, other than null. [00361 In one embodiment of Formula I, X is CH 2 . [00371 Compounds of Formula I include compounds of the general Formula Ia: -N R 3 N Y"/N N R1 (R 4), N x o R2 Ia [00381 or a salt thereof, wherein: [00391 R 1 is H or (1-6C alkyl); WO 2011/006074 PCT/US2010/041538 7 [00401 R 2 is H, (1-6C)alkyl, -(1-6C)fluoroalkyl, -(1-6C)hydroxyalkyl, -(2 6C)dihydroxyalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2

NH

2 , -(1-6C alkyl)NHSO 2 (1-3C alkyl), -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl), -(1-6C alkyl)N(1-4C alkyl) 2 , -(1 6C alkyl)hetCyc', -(1-6C alkyl)hetArl, hetAr 2 , hetCyc 2 , -O(1-6C alkyl), -O(3-6C cycloalkyl), Cyc', or a bridged 7-membered cycloalkyl ring, [0041] or NR 1

R

2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from (1-6C)alkyl, OH, CO 2 H and (1-3C alkyl)CO 2 H; [0042] hetCyc' is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc' is optionally substituted with oxo; [00431 hetCyc 2 is a 6 membered carbon-linked heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc 2 is optionally substituted with F, SO 2

NH

2 , or SO 2 (1-3C alkyl); [0044] hetArl is a 5-membered heteroaryl ring having 1-2 ring heteroatoms independently selected from N and 0 and optionally substituted with (1-4C)alkyl; [00451 hetAr 2 is a 5-6 membered heteroaryl ring having 1-2 ring nitrogen atoms and optionally substituted with one or more substituents independently selected from (1 -4C)alkyl; [00461 Cyc' is a 3-6 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H and -(1-4C alkyl)OH; [00471 Y is (i) phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl), or (ii) a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1 4C)alkyl; [00481 hetCyc 3 is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0; [0049] X is null, -CH 2 -, -CH 2

CH

2 -, -CH 2 0- or -CH 2 NR -; [00501 Rd is H or -(1-4C alkyl); [00511 R 3 is H or -(1-4C alkyl); [0052] each R4 is independently selected from halogen, -(1-4C)alkyl, -OH, -(1 4C)alkoxy, -NH 2 , -NH(1-4C alkyl) and -CH 2 OH; and WO 2011/006074 PCT/US2010/041538 8 [00531 nis0,1,2,3,4,5 or6. [0054] In one embodiment of Formula Ia, X is selected from any of the values described above, other than null. [00551 In one embodiment of Formula Ia, X is CH 2 . [0056] In certain embodiments of Formula I, R 1 is hydrogen. [00571 In certain embodiments of Formula I, R 1 is -(1-6C)alkyl. Examples include methyl, ethyl, propyl and isopropyl. A particular example is methyl. [0058] In certain embodiments of Formula I, R2 is H or -(1-6C)alkyl. [00591 In certain embodiments, R 2 is hydrogen. In one embodiment, R 2 and R 1 are both hydrogen. In one embodiment, R 2 is hydrogen and R 1 is -(1-6C alkyl). [0060] In certain embodiments, R 2 is selected from -(1-6C)alkyl, -(1-6C)fluoroalkyl, (1-6C)difluoroalkyl, -(1-6C)trifluoroalkyl, -(1-6C)chloroalkyl, --(2-6C)chlorofluoroalkyl, -(2-6C)chlorohydroxyalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2

NH

2 , and -(1-6C alkyl)NHSO 2 (1-3C alkyl). [00611 In certain embodiments, R2 is -(1-6C)alkyl. In certain embodiments R2 is selected from methyl, ethyl, propyl, isopropyl, butyl, isobutyl and tert-butyl. Particular examples include methyl, ethyl, isopropyl and tert-butyl. In one embodiment, R 2 is -(1 6C)alkyl and R 1 is hydrogen. In one embodiment, R 2 is -(1-6C)alkyl and R 1 is (1-6C alkyl). [0062] In certain embodiments, R 2 is selected from -(1-6C)fluoroalkyl, -(1 6C)difluoroalkyl, -(1-6C)trifluoroalkyl, -(1-6C)chloroalkyl, --(2-6C)chlorofluoroalkyl, -(2-6C)chlorohydroxyalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2

NH

2 , and -(1-6C alkyl)NHSO 2 (1-3C alkyl). [00631 In certain embodiments, R2 is selected from -(1-6C)fluoroalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2

NH

2 , and -(1-6C alkyl)NHSO 2 (1-3C alkyl). [0064] In certain embodiments, R 2 is -(1-6C)fluoroalkyl. A particular example is C(CH 3

)

2

CH

2 F. In one embodiment, R 2 is -(1-6C)fluoroalkyl and R 1 is hydrogen. In one embodiment, R2 is -(1-6C)fluoroalkyl and R 1 is (1-6C alkyl). [00651 In certain embodiments, R 2 is -(1-6C)difluoroalkyl. Examples include -CHF 2 and -CH 2

CHF

2 . In one embodiment, R 2 is -(1-6C)difluoroalkyl and R 1 is hydrogen. In one embodiment, R2 is -(1-6C)difluoroalkyl and R 1 is (1-6C alkyl). [00661 In certain embodiments, R 2 is -(1-6C)trifluoroalkyl. Examples include CF 3 ,

CH

2

CF

3 and CH(CH 3

)CF

3 . In one embodiment, R 2 is -(1-6C)trifluoroalkyl and R 1 is hydrogen. In one embodiment, R2 is -(1-6C)trifluoroalkyl and R is (1-6C alkyl).

WO 2011/006074 PCT/US2010/041538 9 [00671 In certain embodiments, R 2 is -(1-6C)chloroalkyl. An example includes

CH

2

CH

2 Cl. In one embodiment, R 2 is -(1-6C)chloroalkyl and R 1 is hydrogen. In one embodiment, R2 is -(1-6C)chloroalkyl and R1 is (1-6C alkyl). [00681 In certain embodiments, R 2 is -(1-6C)chlorofluoroalkyl. An example includes

CH

2

CHFCH

2 Cl. In one embodiment, R 2 is -(1-6C)chlorofluoroalkyl and R 1 is hydrogen. In one embodiment, R 2 is -(1-6C)chlorofluoroalkyl and R 1 is (1-6C alkyl). [00691 In certain embodiments, R2 is -(1-6C)difluorochloroalkyl. An example includes -CH 2

CF

2

CH

2 Cl. In one embodiment, R 2 is -(1-6C)difluorochloroalkyl and R 1 is H. In one embodiment, R 2 is -(1-6C)difluorochloroalkyl and R 1 is (1-6C alkyl). [0070] In certain embodiments, R2 is -(2-6C)chlorohydroxyalkyl. An example includes -CH 2

CH(OH)CH

2 Cl. In one embodiment, R2 is -(2-6C)chlorohydroxyalkyl and R 1 is hydrogen. In one embodiment, R 2 is -(2-6C)chlorohydroxyalkyl and R 1 is (1-6C alkyl). [0071] In certain embodiments, R 2 is selected from methyl, ethyl, propyl, isopropyl, isobutyl, tert-butyl, -C(CH 3

)

2

CH

2 F, -CHF 2 , -CH 2

CHF

2 , CF 3 , CH 2

CF

3 , CH(CH 3

)CF

3 ,

CH

2

CH

2 Cl, CH 2

CHFCH

2 Cl, and -CH 2

CF

2

CH

2 Cl. [0072] In certain embodiments, R 2 is selected from methyl, ethyl, propyl, isopropyl,

-CF

3 and -CH 2

CF

3 . [00731 In certain embodiments, R 2 is -(1-6C)hydroxyalkyl or -(2-6C)dihydroxyalkyl. [0074] In certain embodiments, R2 is -(1-6C)hydroxyalkyl. Examples include

-CH

2

CH

2 OH, -CH 2

CH

2

CH

2 OH, -CH 2

CH

2

CH

2

CH

2 OH, -CH 2

CH(OH)CH

3 , -C(CH 3

)

2

CH

2 OH,

-CH

2

C(CH

3

)

2 0H, -CH(CH 3

)CH

2 OH, -CH 2

C(CH

3

)

2

CH

2 OH, -CH(CH 2

OH)CH(CH

3

)

2 ,

-CH(CH

2

CH

3

)CH

2 OH, and -CH(CH 2

OH)C(CH

3

)

3 . A particular example is -CH 2

CH

2 OH. In one embodiment, R 2 is -(1-6C)hydroxyalkyl and R 1 is hydrogen. In one embodiment, R 2 is -(1-6C)hydroxyalkyl and R 1 is -(1-6C alkyl). [0075] In certain embodiments, R2 is -(2-6C)dihydroxyalkyl. Examples include

-CH

2

CH(OH)CH

2 OH, -C(CH 3

)(CH

2

OH)

2 , -CH(CH 2

OH)

2 and -CH(CH 2

OH)(CHOHCH

3 ). Particular examples include -CH 2

CH(OH)CH

2 OH and -C(CH 3

)(CH

2

OH)

2 . In one embodiment, R2 is -(2-6C)dihydroxyalkyl and R 1 is hydrogen. In one embodiment, R 2 is -(2 6C)dihydroxyalkyl and R 1 is -(1-6C alkyl). [00761 In certain embodiments, R 2 is -(1-6C alkyl)CN. Particular examples include

-CH

2 CN and -C(CH 3

)

2 CN. In one embodiment, R 2 is -(1-6C alkyl)CN and R 1 is hydrogen. In one embodiment, R 2 is -(1-6C alkyl)CN and R 1 is (1-6C alkyl).

WO 2011/006074 PCT/US2010/041538 10 [00771 In certain embodiments, R 2 is -(1-6C alkyl)SO 2

NH

2 . A particular example is CH 2

CH

2

SO

2

NH

2 . In one embodiment, R 2 is -(1-6C alkyl)SO 2

NH

2 and R 1 is hydrogen. In one embodiment, R 2 is -(1-6C alkyl)SO 2

NH

2 and R 1 is (1-6C alkyl). [0078] In certain embodiments, R2 is -(1-6C alkyl)NHSO 2 (1-3C alkyl). Particular examples include -CH 2

CH

2

NHSO

2

CH

3 and -C(CH 3

)

2

CH

2

NHSO

2

CH

3 . In one embodiment, R2 is -(1-6C alkyl)NHSO 2 (1-3C alkyl) and R 1 is hydrogen. In one embodiment, R2 is -(1-6C alkyl)NHSO 2 (1-3C alkyl) and R 1 is (1-6C alkyl). [0079] In certain embodiments, R2 is selected from -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl) and -(1-6C alkyl)N(1-4C alkyl) 2 . [00801 In certain embodiments, R2 is -(1-6C alkyl)NH 2 . Examples include

-CH

2

C(CH

3

)

2

NH

2 and -CH 2

CH

2

CH

2

NH

2 . A particular example is -CH 2

C(CH

3

)

2

NH

2 . In one embodiment, R2 is -(1-6C alkyl)NH 2 and R 1 is hydrogen. In one embodiment, R2 is -(1-6C alkyl)NH 2 and R 1 is (1-6C alkyl). [00811 In certain embodiments, R2 is -(1-6C alkyl)NH(1-4C alkyl). Examples include groups having the formula -(1-4C alkyl)NHCH 3 . A particular value is -C(CH3)2NHCH3. In one embodiment, R2 is -(1-6C alkyl)NH(1-4C alkyl) and R is hydrogen. In one embodiment, R2 is -(1-6C alkyl)NH(1-4C alkyl) and R1 is (1-6C alkyl). [0082] In certain embodiments, R 2 is -(1-6C alkyl)N(1-4C alkyl) 2 . Examples include groups having the formula -(1-4C alkyl)N(CH 3

)

2 . A particular value is -(1-6C alkyl)NMe 2 . In one embodiment, R2 is -(1-6C alkyl)N(1-4C alkyl) 2 and R 1 is hydrogen. In one embodiment, R2 is -(1-6C alkyl)N(1-4C alkyl) 2 and R 1 is (1-6C alkyl). [00831 In certain embodiments, R2 is -(1-6C alkyl)NHC(=0)O(1-4C alkyl). An example includes CH 2

CH

2

CH

2

NHC(=O)OC(CH

3

)

3 . In one embodiment, R 2 is -(1-6C alkyl)NHC(=0)O(1-4C alkyl) and R 1 is hydrogen. In one embodiment, R 2 is -(1-6C alkyl)NHC(=0)O(1-4C alkyl) and R 1 is (1-6C alkyl). [0084] In certain embodiments, R 2 is selected from -(1-6C alkyl)hetCyc' and -(1-6C alkyl)hetArl. [0085] In certain embodiments, R2 is -(1-6C alkyl)hetCyc'. Examples of hetCyc' rings include morpholinyl, piperidinyl, piperazinyl and imidazolidinyl, each of which is optionally substituted with a substituent selected from oxo, OH, halogen, and (1-6C)alkyl. In certain embodiments hetCyc' is morpholinyl, piperidinyl, piperazinyl or imidazolidin-2-one optionally substituted with OH, halogen or (1-6C)alkyl. Examples of the -(1-6C)alkyl portion include methylene, ethylene, dimethylethylene, and the like.

WO 2011/006074 PCT/US2010/041538 11 [00861 Examples of R 2 when represented by -(1-6C alkyl)hetCyc' include the structures: N N N 0 NH NH N N N OH [0087] In certain embodiments, R 2 when represented by -(1-6C alkyl)hetCyc' includes the structures: 0 0 [00881 In certain embodiments hetCyc is morpholinyl or imidazolidin-2-one. [00891 In one embodiment, R 2 is -(1-6C alkyl)hetCyc' and R 1 is hydrogen. In one embodiment, R2 is-(1-6C alkyl)hetCyc' and R 1 is (1-6C alkyl). [0090] In certain embodiments, R2 is -(1-6C alkyl)hetAr . Examples of hetArl include furanyl, pyrazolyl, and imidazolyl rings which are optionally substituted with -(1-4C alkyl), for example methyl. Examples of the -(1-6C)alkyl portion include methylene, ethylene, dimethylmethylene, and the like. Examples of R 2 when represented by -(1-6C alkyl)hetArl include the structures: -N /,s 0~ N N 0/ /!> N N H N NK. [0091] Particular values for R 2 when represented by -(1-6C alkyl)hetArl include the structures: WO 2011/006074 PCT/US2010/041538 12 N / N N, [00921 In one embodiment, R 2 is -(1-6C alkyl)hetArl and R 1 is hydrogen. In one embodiment, R2 is -(1-6C alkyl)hetArl and R 1 is (1-6C alkyl). [00931 In certain embodiments, R 2 is hetAr 2 . Examples of hetAr 2 include pyridyl, pyrazolyl and imidazolyl rings optionally substituted with one or more substituents independently selected from (1-4C)alkyl, (3-6C)cycloalkyl, halogen and OH. Particular examples of hetAr 2 substituents include methyl, ethyl, isopropyl, cyclopropyl, fluoro and hydroxy. Particular examples of hetAr 2 include the structures: N N N - N N N HN F OH 0 NH NH NH -N IN N N / N N N N H [0094] In certain embodiments hetAr 2 is a pyridyl or pyrazolyl ring optionally substituted with one or more substituents independently selected from -(1-4C)alkyl, for example one or more methyl groups, for example 1 or 2 methyl groups. Particular examples of hetAr 2 include the structures: WO 2011/006074 PCT/US2010/041538 13 N N N [00951 In one embodiment, R 2 is hetAr 2 and R 1 is hydrogen. In one embodiment, R 2 is hetAr 2 and R 1 is (1-6C alkyl). [00961 In certain embodiments, R2 is hetCyc 2 . Examples of hetCyc 2 include piperidinyl and tetrahydropyranyl rings optionally substituted with F, SO 2

NH

2 or S0 2 (1-3C alkyl). Particular examples of R 2 when represented by hetCyc 2 include the structures: F NH N N

SO

2

NH

2 S02CH 3 [00971 In one embodiment, R 2 is hetCyc 2 and R 1 is hydrogen. In one embodiment, R2 is hetCyc 2 and R 1 is (1-6C alkyl). [00981 In certain embodiments, R 2 is -O(1-6C alkyl) which is optionally substituted with halogen, OH or (1-4C)alkoxy. Examples include -OMe, -OEt, -OCH 2

CH

2 0C(CH 3

)

3 , OCH 2

CH

2 Br, -OCH 2

CH

2 Cl and -OCH 2

CH

2 OH. In one embodiment, R2 is -O(1-6C alkyl) which is optionally substituted with halogen, OH or (1-4C)alkoxy, and R 1 is hydrogen. In one embodiment, R 2 is -O(1-6C alkyl) which is optionally substituted with halogen, OH or (1-4C)alkoxy, and R 1 is (1-6C alkyl). [0099] In certain embodiments, R2 is -0(1-6C alkyl). Particular examples include OMe and OEt. [00100] In certain embodiments, R 2 is -O(3-6C cycloalkyl). A particular example is cyclopropoxy. In one embodiment, R 2 is -O(3-6C cycloalkyl) and R 1 is hydrogen. In one embodiment, R2 is -O(3-6C cycloalkyl) and R 1 is (1-6C alkyl). [00101] In certain embodiments, R 2 is -O(1-6C alkyl) or -O(3-6C cycloalkyl) [00102] In certain embodiments, R 2 is CycI or a bridged 7-membered cycloalkyl ring. [001031 In certain embodiments, R2 is Cyc', wherein Cyc' is a 3-6 membered cycloalkyl ring optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H, -(1-4C alkyl)OH, halogen and CF 3 . In one embodiment, Cyc' is optionally substituted with one or more substituents independently selected from methyl, -OH, -OMe, -CO 2 H, CH 2 OH, CH 2

CH

2 OH and CF 3 . In certain WO 2011/006074 PCT/US2010/041538 14 embodiments, R 2 is Cyc', wherein the cycloalkyl ring is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H and -(1 4C alkyl)OH, such as one or more substituents independently selected from methyl, -OH, CH 2 OH and -CO 2 H. In one embodiment, Cyc' is optionally substituted with one or more substituents independently selected from methyl, -OH, -CH 2 OH and -CO 2 H. In one embodiment, Cyc' is optionally substituted with one or two of said substituents. [00104] Examples of R 2 when represented by Cyc' include the structures: OH $ 02H '

SCF

3 ~~H OH OH C0 2 H OH OH OH [00105] Particular examples of R 2 when represented by Cyc' include the structures: 0H 2 CO2H OH

CO

2 H OH HO [00106] In one embodiment of Formula I, Cyc' is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from (1-4C alkyl), -OH, -OMe, -CO 2 H, -(1-4C alkyl)OH, halogen and CF 3

.

WO 2011/006074 PCT/US2010/041538 15 [001071 In one embodiment of Formula I, Cyc' is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, OMe, -CO 2 H and -(1-4C alkyl)OH. [00108] In one embodiment, R 2 is cyclopropyl. [00109] In one embodiment R 2 is selected from the structures: O H Ix CO2H CF3 [00110] In one embodiment, R 2 is Cyc' and R 1 is hydrogen. In one embodiment, R 2 is Cyc' and R 1 is (1-6C alkyl). [00111] In one embodiment, R 2 is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H, -(1-4C alkyl)OH, halogen and CF 3 . [00112] In one embodiment, R 2 is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, OMe, -CO 2 H and -(1-4C alkyl)OH. [00113] In one embodiment, R 2 is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from methyl, CO 2 H, and CH 2 OH. [00114] In certain embodiments, R 2 is cyclopropyl optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H, -(1-4C alkyl)OH, halogen and CF 3 . [001151 In certain embodiments, R 2 is cyclopropyl optionally substituted with one or more substituents independently selected from methyl, -CO 2 H, and CH 2 OH. [00116] In certain embodiments, R 2 is cyclobutyl optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H, -(1-4C alkyl)OH, halogen and CF 3 . In certain embodiments, R 2 is cyclobutyl optionally substituted with one or more substituents independently selected from methyl, -OH, -OMe, -CO 2 H,

CH

2 OH, CH 2

CH

2 OH and CF 3 . [001171 In certain embodiments, R 2 is cyclopentyl optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H, -(1-4C WO 2011/006074 PCT/US2010/041538 16 alkyl)OH, halogen and CF 3 . In certain embodiments, R 2 is cyclopentyl optionally substituted with one or more substituents independently selected from methyl, -OH, -OMe, -CO 2 H,

CH

2 OH, CH 2

CH

2 OH and CF 3 . [001181 In certain embodiments, R 2 is cyclohexyl optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H, -(1-4C alkyl)OH, halogen and CF 3 . In certain embodiments, R 2 is cyclohexyl optionally substituted with one or more substituents independently selected from methyl, -OH, -OMe, -CO 2 H,

CH

2 OH, CH 2

CH

2 OH and CF 3 . [00119] In certain embodiments, R 2 is -(1-6C alkyl)(3-6C cycloalkyl). Examples of the (1-6C alkyl) portion include methyl, ethyl, propyl and butyl. Examples of the cycloalkyl portion include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In one embodiment, the cycloalkyl portion is cyclopropyl. Particular examples include the structures: [00120] In one embodiment, R 2 is -(1-6C alkyl)(3-6C cycloalkyl) and R 1 is hydrogen. In one embodiment, R 2 is -(1-6C alkyl)(3-6C cycloalkyl) and R 1 is (1-6C alkyl). [00121] In certain embodiments, R 2 is -(1-6Calkyl)(1-4C alkoxy). Examples include

CH

2

CH

2 0CH 3 and CH(CH 3

)CH

2 0CH 3 . In one embodiment, R2 is -(1-6Calkyl)(1-4C alkoxy) and R 1 is hydrogen. In one embodiment, R 2 is -(1-6Calkyl)(1-4C alkoxy) and R 1 is (1-6C alkyl). [00122] In certain embodiments, R2 is -(1-6C hydroxyalkyl)(1-4C alkoxy). An example includes -CH 2

CH(OH)CH

2 0CH 3 . In one embodiment, R 2 is -(1-6C hydroxyalkyl)(1-4C alkoxy) and R 1 is hydrogen. In one embodiment, R 2 is -(1-6C hydroxyalkyl)(1-4C alkoxy) and R 1 is (1-6C alkyl). [001231 In certain embodiments, R2 is a bridged 7-membered cycloalkyl ring. In certain embodiments, R2 is a bridged 7-membered cycloalkyl ring optionally substituted with (1-6C)hydroxyalkyl. In certain embodiments, R 2 is a bridged 7-membered cycloalkyl ring optionally substituted with hydroxymethyl. Examples of R 2 include the structures: OH [00124] A particular example or R2 is the structure: WO 2011/006074 PCT/US2010/041538 17 [001251 In one embodiment, R 2 is a bridged 7-membered cycloalkyl ring optionally substituted with (1-6C)hydroxyalkyl and R 1 is hydrogen. In one embodiment, R 2 is a bridged 7-membered cycloalkyl ring and R 1 is (1-6C alkyl). [00126] In certain embodiments, R2 is a bridged 7-8 membered heterocyclic ring having 1-2 ring nitrogen atoms. A particular example is the structure: N [001271 In one embodiment, R 2 is a bridged 7-8 membered heterocyclic ring having 1 2 ring nitrogen atoms and R 1 is hydrogen. In one embodiment, R 2 is a bridged 7-8 membered heterocyclic ring having 1-2 ring nitrogen atoms and R 1 is (1-6C alkyl). [00128] In certain embodiments, NR 1

R

2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from (1-6C)alkyl, OH, CO2H, (1-3C alkyl)CO 2 H, -O(1-6C alkyl) and (1-6C)hydroxyalkyl. Examples include 4-6 membered azacyclic rings optionally substituted with one or more groups independently selected from methyl, OH, -C(=O)OH, -CH 2 COOH, OMe, and -CH 2 OH. In certain embodiments, the azacyclic ring is optionally substituted with one or two of said substituents. Particular examples include the structures: OHO H NHN O H N N N OH N N CO 2 H N CO 2 H [00129] In certain embodiments, NR 1

R

2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from -(1 6C)alkyl, -OH, -CO 2 H and -(1-3C alkyl)CO 2 H. Examples include 4-6 membered azacyclic rings optionally substituted with one or two groups independently selected from methyl, OH, -C(=O)OH and -CH 2 COOH. Particular examples include the structures: WO 2011/006074 PCT/US2010/041538 18 N OH N OH N OH N CO2HN CO 2 H [001301 Compounds of Formula I also include compounds wherein: [001311 R 1 is H or -(1-6C alkyl); [00132] R2 is H, -(1-6C)alkyl, -(1-6C)fluoroalkyl, -(1-6C)hydroxyalkyl, -(2 6C)dihydroxyalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2

NH

2 , -(1-6C alkyl)NHSO 2 (1-3C alkyl), -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl), -(1-6C alkyl)N(1-4C alkyl) 2 , -(1 6C alkyl)hetCyc', -(1-6C alkyl)hetArl, hetAr 2 , -O(1-6C alkyl), -O(3-6C cycloalkyl), or a 3, 4 or 5 membered cycloalkyl ring optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, OMe, -CO 2 H and -(1-4C alkyl)OH; [001331 or NR R2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from -(1-6C)alkyl, -OH, -CO 2 H and -(1-3C alkyl)CO 2 H; [00134] and X, Y, R3, R and n are as defined for Formula I. [001351 Compounds of Formula I also include compounds wherein: [001361 R 1 is H or -(1-6C alkyl); [001371 R2 is H, -(1-6C)alkyl, -(1-6C)fluoroalkyl, -(1-6C)hydroxyalkyl, -(2 6C)dihydroxyalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2

NH

2 , -(1-6C alkyl)NHSO 2 (1-3C alkyl), -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl), -(1-6C alkyl)N(1-4C alkyl) 2 , -(1 6C alkyl)hetCyc', -(1-6C alkyl)hetArl, hetAr 2 , hetCyc 2 , -O(1-6C alkyl), -O(3-6C cycloalkyl), or a bridged 7-membered cycloalkyl ring, [001381 or NR R2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from -(1-6C)alkyl, -OH, -CO 2 H and -(1-3C alkyl)CO 2 H; and [001391 and X, Y, R3, R and n are as defined for Formula I. [00140] Referring now to the substituents on the ring at the 5-position of Formula I, wherein the 5-position is identified in the following structure:

R

3 N' YU ' 7-N N R1 (R4 N X 0 R 2 WO 2011/006074 PCT/US2010/041538 19 [00141] in one embodiment Y is phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 , -(1-4C alkyl)hetCyc 3 , -O(1-4C alkyl)O(1-3C alkyl) and -O(3-6C dihydroxyalkyl). [00142] In one embodiment, Y is phenyl optionally substituted with one or two of said substituents. In one embodiment Y is phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -O(1-4C alkyl)hetCyc 3 , -(1 4C alkyl)hetCyc 3 , -O(1-4C alkyl)O(1-3C alkyl) and -O(3-6C dihydroxyalkyl). In one embodiment, Y is phenyl optionally substituted with one or two of said substituents. [001431 In one embodiment, Y is phenyl optionally substituted with one or more substituents independently selected from -F, -Cl, -OMe, -CF 3 , -CHF 2 , morpholinylethoxy, morpholinylethyl, -OCH 2

CH

2 OMe, 2,3-dihydroxypropoxy and 2,2-dimethyl-1,3-dioxolanyl. In one embodiment, Y is phenyl optionally substituted with one or two of said substituents. [00144] The term "morpholinylethoxy" as used herein refers to a morpholinyl ring substituted at the nitrogen ring atom with an ethoxy group and can be represented by the structure: 0 N [001451 The term "morpholinylethyl" as used herein refers to a morpholinyl ring substituted at the nitrogen ring atom with an ethyl group and can be represented by the structure: 0 N [001461 Example of Y include phenyl, 3-fluorophenyl, 2,5-difluorophenyl, 2-chloro-5 fluorophenyl, 2-methoxyphenyl, 2-methoxy-5-fluorophenyl, 2-trifluoromethyl-5-fluoro phenyl, 2-difluoromethyl-5-fluorophenyl, 3-chloro-5-fluorophenyl, 3-fluoro-5-(2-morpho linylethoxy)phenyl, 3-fluoro-5-(2-morpholinylethyl)phenyl, 5-fluoro-2-(2-morpholinyl ethyl)phenyl, 3-fluoro-5-methoxyethoxyphenyl, 5-fluoro-2-methoxyethoxyphenyl, 3-fluoro 5-(2,3-dihydroxypropoxy)phenyl, 2-(2,3-dihydroxypropoxy)-5-fluorophenyl, WO 2011/006074 PCT/US2010/041538 20 F 0 FF /<o , and 0 [00147] The terms "3-fluoro-5-(2-morpholinylethoxy)phenyl", "3-fluoro-5-(2 morpholinylethyl)phenyl" and "5-fluoro-2-(2-morpholinylethyl)phenyl" can be represented by the structures: F N F Ks N F N respectively. [00148] In one embodiment, Y is fluorophenyl optionally substituted with a substituent selected from -O(1-4C alkyl)hetCyc 3 , -(1-4C alkyl)hetCyc 3 , -O(1-4C alkyl)O(1-3C alkyl) and -O(3-6C dihydroxyalkyl). [00149] In one embodiment, Y is fluorophenyl substituted with a substituent selected from morpholinylethoxy, morpholinylethyl, -OCH 2

CH

2 OMe, 2,3-dihydroxypropoxy and 2,2 dimethyl-1,3-dioxolanyl. [001501 In one embodiment, Y is selected from 3-fluoro-5-(2 morpholinylethoxy)phenyl, 5-fluoro-2-(2-morpholinoethoxy)phenyl, 3-fluoro-5 methoxyethoxyphenyl, 3-fluoro-5-(2-morpholinylethyl)phenyl, 5-fluoro-2-(2 morpholinylethyl)phenyl, 3-fluoro-5-(2,3-dihydroxypropoxy)phenyl, 2-(2,3 dihydroxypropoxy)-5-fluorophenyl, oF F 0 F and 0 [001511 In one embodiment Y is phenyl optionally substituted with one or more substituents independently selected from halogen, -(1-4C)alkoxy, -CF 3 , -CHF 2 , -O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl). [00152] In one embodiment, Y is phenyl optionally substituted with one or more substituents independently selected from -F, -Cl, -OMe, -CF 3 , -CHF 2 , morpholinylethoxy and

-OCH

2

CH

2 OMe. In certain embodiments, Y is phenyl optionally substituted with one or two WO 2011/006074 PCT/US2010/041538 21 of said substituents. Particular values for Y include phenyl, 3-fluorophenyl, 2,5 difluorophenyl, 2-chloro-5-fluorophenyl, 2-methoxyphenyl, 2-methoxy-5-fluorophenyl, 2 trifluoromethyl-5-fluorophenyl, 2-difluoromethyl-5-fluorophenyl, 3-chloro-5-fluorophenyl, 3-fluoro-5-(2-morpholinylethoxy)phenyl, 5-fluoro-2-(2-morpholinoethoxy)phenyl, 3-fluoro 5-methoxyethoxyphenyl and 5-fluoro-2-methoxyethoxyphenyl. [00153] In one embodiment, Y is a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl), (1-4C)alkyl and NH 2 . Examples include pyridyl and thienyl groups optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl), (1-4C)alkyl and

NH

2 . [00154] In certain embodiments, Y is pyridyl optionally substituted with one or more substituents independently selected from fluoro, chloro, methoxy, methyl, ethyl, and amino. [001551 In certain embodiments Y is pyrid-2-yl, pyrid-3-yl, 5-fluoropyrid-3-yl, 2 methoxy-5-fluoropyridy-3-yl, 2-chloro-5-fluoropyridy-3-yl, 2-methyl-5-fluoropyrid-3-yl, 2 ethyl-5-fluoropyrid-3-yl or 2-amino-5-fluoropyrid-3-yl. [00156] In certain embodiments, Y is pyridyl substituted with one or more substituents independently selected from halogen, (1-4C)alkyl and amino. [001571 In certain embodiments, Y is pyridyl substituted with one or more substituents independently selected from halogen and (1-4C)alkyl. [00158] In certain embodiments, Y is pyridyl substituted with one or more substituents independently selected from fluoro, chloro, methyl and ethyl. [001591 In certain embodiments, Y is pyridyl substituted with one or more substituents independently selected from F, methyl and ethyl. [00160] In certain embodiments, Y is 5-fluoropyrid-3-yl, 2-methyl-5-fluoropyrid-3-yl or 2-ethyl-5-fluoropyrid-3-yl. [00161] In certain embodiments, Y is 5-fluoropyrid-3-yl. [00162] In one embodiment, Y is a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1 4C)alkyl. Examples include pyridyl and thienyl groups optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1-4C)alkyl, for example one or more substituents independently selected from fluoro, methoxy and methyl.

WO 2011/006074 PCT/US2010/041538 22 Particular values for Y include pyrid-2-yl, pyrid-3-yl, 5-fluoropyrid-3-yl, 2-methoxy-5 fluoropyridy-3-yl and 2-methyl-5-fluoropyridy-3-yl. [00163] In one embodiment, Y is a pyrid-2-on-3-yl ring optionally substituted with one or more substituents independently selected from halogen and (1-4C)alkyl. Examples include pyrid-2-on-3-yl rings optionally substituted with one or more substituents independently selected from fluoro and methyl. In certain embodiments the pyrid-2-on-3-yl ring is optionally substituted with one or two of said substituents. In one embodiment, Y is 5-fluoropyridin-2(1H)-one optionally substituted with (1-4C)alkyl, for example methyl. Particular values for Y include the structures: HN F N F [00164] In one embodiment, the Y group has the absolute configuration shown in Figure Ia: N-N R3N 'R4 N N R1 x 0 R2 Ia [001651 wherein R1, R , R , R4, X, Y and n are as defined herein. [00166] With reference to the R 3 substituent, in one embodiment R 3 is H. [001671 In one embodiment, R3 is -(1-4C)alkyl, for example, methyl, ethyl, propyl, isopropyl or butyl. In one embodiment, R 3 is methyl. [00168] With reference to the R 4 substituent, in one embodiment R4 is halogen. Particular examples are fluoro and chloro. [00169] In one embodiment, R4 is -(1-4C)alkyl, such as methyl, ethyl, propyl, isopropyl, or butyl. A particular example is methyl. [001701 In one embodiment, R 4 is -OH. [001711 In one embodiment, R 4 is (1-4 C)alkoxy, for example -OMe and -OEt. [00172] In one embodiment, R4 is -NH 2 . [00173] In one embodiment, R4 is -NH(1-4C alkyl), for example -NHMe, -NHEt, NHPr, -NHiPr or -NHBu. A particular example is -NHMe. [00174] In one embodiment, R4 is CH 2

OH.

WO 2011/006074 PCT/US2010/041538 23 [001751 In one embodiment, each R4 is independently selected from -F, -Cl, -OH, OMe, -NH 2 , -Me, -CH 2 OH and -NHMe. [00176] In one embodiment, n is 0, 1, 2, 3 or 4. In one embodiment, n is 0, 1, 2 or 3. In one embodiment, n is 0, 1 or 2. [001771 In one embodiment, n is 0. [00178] In one embodiment, n is 1. [001791 In one embodiment, n is 2. [00180] With reference to the heterocyclic ring directly attached to the 5-position of Formula I, in certain embodiments, X is null, -CH 2 - or -CH 2

CH

2 -. [00181] In one embodiment X is null, such that the heterocyclic ring at the 5-position of Formula I has the structure: yR3 N (R 4) [00182] where R3, R 4 , Y and n are as defined herein. In one embodiment, Y is phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3 and -CHF 2 . In one embodiment, Y is phenyl, 3-fluorophenyl and 2,5 difluorophenyl. In one embodiment, Y is 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1 4C)alkyl, for example one or more halogen atoms. In one embodiment, Y is pyridyl. In one embodiment, R 3 is hydrogen. In another embodiment, R3 is methyl. In one embodiment, n is 0. A particular example of the ring at the 5-position of Formula I when X is null includes the structures: F F N N F N [00183] In one embodiment, X is CH 2 , such that the heterocyclic ring at the 5-position of Formula I has the structure: WO 2011/006074 PCT/US2010/041538 24

R

3 YP I ( N [00184] where R3, R 4 , Y and n are as defined herein. In one embodiment, X is CH 2 , R3, R4 and n are as defined herein, and Y is phenyl optionally substituted with one or more substituents independently selected from -F, -Cl, -OMe, -CF 3 , -CHF 2 , morpholinylethoxy, morpholinylethyl, -OCH 2

CH

2 OMe, 2,3-dihydroxypropoxy and 2,2-dimethyl-1,3-dioxolanyl. [001851 In one embodiment, X is CH 2 , R3, R4 and n are as defined herein, and Y is phenyl, 3-fluorophenyl, 2,5-difluorophenyl, 2-chloro-5-fluorophenyl, 2-methoxyphenyl, 2 methoxy-5-fluorophenyl, 2-trifluoromethyl-5-fluorophenyl, 2-difluoromethyl-5-fluorophenyl, 3-chloro-5-fluorophenyl, 3-fluoro-5-(2-morpholinylethoxy)phenyl, 3-fluoro-5-(2 morpholinylethyl)phenyl, 5-fluoro-2-(2-morpholinylethyl)phenyl, 3-fluoro-5 methoxyethoxyphenyl, 5-fluoro-2-methoxyethoxyphenyl, 3-fluoro-5-(2,3 dihydroxypropoxy)phenyl, 2-(2,3-dihydroxypropoxy)-5-fluorophenyl, F 0/ F 0 0 . '' or [00186] In one embodiment, X is CH 2 , R3, R4 and n are as defined herein, and Y is fluorophenyl substituted with a substituent selected from morpholinylethoxy, OCH 2

CH

2 OMe, 2,3-dihydroxypropoxy and 2,2-dimethyl-1,3-dioxolanyl. [001871 In one embodiment, X is CH 2 , Y and R4 are as defined herein, and R3 is hydrogen. In another embodiment, X is CH 2 , Y and R4 are as defined herein, and R3 is methyl. In one embodiment, each R4 is independently selected from F, Cl, Me, OH, OMe,

NH

2 , NHMe, CH 2 OH, CHF 2 and CF 3 . In one embodiment, n is 0. In one embodiment, n is 1. In one embodiment, n is 2. [001881 In one embodiment X is CH 2 , R3, R4 and n are as defined herein, and Y is a 5 6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl), (1-4C)alkyl and NH 2 . [001891 In one embodiment, X is CH 2 , R3, R4 and n are as defined herein, and Y is pyrid-2-yl, pyrid-3-yl, 5-fluoropyrid-3-yl, 2-chloro-5-fluoropyridy-3-yl, 2-methyl-5- WO 2011/006074 PCT/US2010/041538 25 fluoropyrid-3-yl, or 2-ethyl-5-fluoropyrid-3-yl. In one embodiment, R 3 is hydrogen. In another embodiment, R3 is methyl. In one embodiment, each R4 is independently selected from F, Cl, Me, OH, OMe, NH 2 , NHMe, CH 2 OH, CHF 2 and CF 3 . In one embodiment, n is 0. In one embodiment, n is 1. In one embodiment, n is 2. [00190] In one embodiment, X is CH 2 , R3, R4 and n are as defined herein, and Y is pyridyl optionally substituted with one or more substituents independently selected from halogen and (1-4C)alkyl. [00191] In one embodiment, X is CH 2 , R3, R4 and n are as defined herein, and Y is pyridyl optionally substituted with one or more substituents independently selected from fluoro, methyl and ethyl. [00192] In one embodiment, X is CH 2 , R3, R4 and n are as defined herein, and Y is 5 fluoropyrid-3-yl, 2-methyl-5-fluoropyrid-3-yl, or 2-ethyl-5-fluoropyrid-3-yl. In one embodiment, R3 is hydrogen. [00193] In one embodiment, X is CH 2 , R3, R4 and n are as defined herein, and Y is a pyrid-2-on-3-yl ring optionally substituted with one or more substituents independently selected from halogen and (1-4C)alkyl. [00194] In one embodiment, X is CH 2 , R3, R 4 and n are as defined herein, and Y is a pyrid-2-on-3-yl ring optionally substituted with one or more groups selected from methyl and fluoro. In one embodiment, Y is 5-fluoropyridin-2(1H)-one optionally substituted with methyl. In one embodiment, R 3 is hydrogen. In another embodiment, R 3 is methyl. In one embodiment, each R4 is independently selected from F, Cl, Me, OH, OMe, NH 2 , NHMe,

CH

2 OH, CHF 2 and CF 3 . In one embodiment, n is 0. In one embodiment, n is 1. In one embodiment, n is 2. [001951 In one embodiment the ring at the 5-position of Formula I when X is CH 2 include the structures: F CI F OMe -~~ \1 F F DZ/-N F N N N WO 2011/006074 PCT/US2010/041538 26 F F F F N F N N o N 00 OH HO3 F F F HO--H N N N OH FF F F N F [ N Xs uh N N F F FhF Nd-N /HN/ N T N' T N' OH 0Y F N F N N NA [001961 In one embodiment, X is CH 2 , such that the heterocyclic ring at the 5-position of Formula I has the structure:

N

WO 2011/006074 PCT/US2010/041538 27 [001971 where R3, R 4 , Y and n are as defined herein. In one embodiment Y is phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 or -O(1-4C alkyl)O(1-3C alkyl). In one embodiment, Y is phenyl, 3-fluorophenyl, 2,5-difluorophenyl, 2-difluoromethyl-5 fluorophenyl, 2-trifluoromethyl-5-fluorophenyl, 2-chloro-5-fluorophenyl, 3-chloro-5 fluorophenyl, 2-methoxy-5-fluorophenyl, 3-fluoro-5-methoxyethoxyphenyl, 3-fluoro-5-(2 morpholinylethoxy)phenyl, 5-fluoro-2-(2-morpholinoethoxy)phenyl, or 5-fluoro-2 methoxyethoxyphenyl. In one embodiment, Y is a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1 4C)alkyl. In one embodiment, R 3 is hydrogen. In another embodiment, R3 is methyl. In one embodiment, each R4 is independently selected from F, Cl, Me, OH, OMe, NH 2 , NHMe,

CH

2 OH, CHF 2 and CF 3 . In one embodiment, Y is pyrid-2-yl, 5-fluoropyrid-3-yl or 2 methoxy-5-fluoropyridy-3-yl. In one embodiment, n is 0, 1 or 2. [00198] Particular examples of the ring at the 5-position of Formula I when X is CH 2 include the structures: '12 - 12 N F N F N F N OH OMe

NH

2 F F F F NN N N F F CI F F F F F N N N N HO OH HNs

CHF

2

CF

3 CI N F N F N F N WO 2011/006074 PCT/US2010/041538 28 F CI OMe F le, 0_ F N F N N N N F F F 0 N O H3CO N N N N F F N 0 N HO N [00199] In one embodiment, X is -CH 2

CH

2 -, such that the heterocyclic ring at the 5 position of Formula I has the structure: Y R3 N (R4 [00200] where R3, R 4 , Y and n are as defined herein. In one embodiment, Y is phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3 and -CHF 2 . In one embodiment, Y is phenyl or 3-fluorophenyl. In one embodiment, Y is a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1-4C)alkyl. In one embodiment, Y is pyridyl optionally substituted with one or more F atoms. In one embodiment, R 3 is hydrogen. In another embodiment, R3 is methyl. In one embodiment, n is 0, 1 or 2. In one embodiment, n is 0. Particular examples of the ring at the 5-position of Formula I when X is

-CH

2

CH

2 - include the structures: WO 2011/006074 PCT/US2010/041538 29 \ / F/ N N [00201] In one embodiment, X is -CH 2 0-. In one embodiment, the heterocyclic ring at the 5-position of Formula I has the structure: N 0 O4R4 [00202] where R3, R 4 , Y and n are as defined herein. In one embodiment, Y is phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3 and -CHF 2 . In one embodiment, Y is phenyl optionally substituted with one or more substituents independently selected from -F and -(1 -4C)alkoxy. In one embodiment, Y is phenyl, 3-fluorophenyl, 2,5-difluorophenyl, or 2-methoxyphenyl. In one embodiment, Y is a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1-4C)alkyl, for example one or more halogen atoms. In one embodiment, Y is pyrid-3-yl. In one embodiment, R 3 is hydrogen. In another embodiment, R3 is methyl. In one embodiment, n is 0, 1 or 2. Particular examples of the ring at the 5-position of Formula I when X is -CH 2 0- include the structures: N / \ N N N oj 0 0 F F N N -0 N [00203] In one embodiment, X is -CH 2 NRd-. In one embodiment, the heterocyclic ring at the 5-position of Formula I has the structure: WO 2011/006074 PCT/US2010/041538 30

R

3 Y N Rd [00204] where R 3 , R 4 , Y, Rd and n are as defined herein. In one embodiment, Rd is H. In one embodiment, Rd is -(1-4C alkyl), for example methyl, ethyl, propyl, isopropyl, or butyl. A particular example is methyl. In one embodiment, Y is phenyl optionally substituted with one or more substituents independently selected from halogen, -(1 4C)alkoxy, -CF 3 and -CHF 2 . In one embodiment, Y is a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1-4C)alkyl. In one embodiment, Y is pyridyl optionally substituted with one or more F atoms. In one embodiment, n is 0. Particular examples of the ring at the 5-position of Formula I when X is -CH 2 NR - include the structures: // \ \ / F F F N N N N HN HN N HN [002051 It will be appreciated that certain compounds according to the invention may contain one or more centers of asymmetry and may therefore be prepared and isolated as a mixture of isomers such as a racemic or diastereomeric mixture, or in an enantiomerically or diastereomerically pure form. [00206] The compounds of Formula I also include compounds of Formula lb

R

3 N 7-N N

(

4 ).-N ) NR1 (R N X 0 R 2 lb [002071 and salts thereof, wherein: [00208] R 1 is H; [00209] R2 is H, (1-6C)alkyl, (1-6C)fluoroalkyl, -(1-6C)hydroxyalkyl or -(2-6C)dihydroxyalkyl; WO 2011/006074 PCT/US2010/041538 31 [00210] Y is (i) phenyl optionally substituted with one or more substituents independently selected from halogen, -(1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl) or (ii) a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1 4C)alkyl; [00211] hetCyc 3 is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0; [00212] X is CH 2 or CH 2

CH

2 ; [00213] R3 is H; [00214] each R4 is independently selected from halogen, -(1-4C)alkyl, -OH, -(1 4C)alkoxy, -NH 2 , -NH(1-4C alkyl) and -CH 2 OH; and [002151 nis0, 1,or2. [00216] In certain embodiments of Formula Ib, R 2 is H, (1-6C)alkyl, -(1 6C)hydroxyalkyl or -(2-6C)dihydroxyalkyl; Y is phenyl optionally substituted with one or more substituents independently selected from -F, -Cl, -OMe, -CF 3 , -CHF 2 , morpholinylethoxy and -OCH 2

CH

2 OMe; X is CH 2 and n is 0. [002171 In certain embodiments of Formula Ib, R 2 is H, methyl, ethyl, isopropyl, tert butyl, CH 2

CH

2 OH, or CH 2

CH(OH)CH

2 OH; Y is phenyl, 3-fluorophenyl, 2,5-difluorophenyl, 2-chloro-5-fluorophenyl, 2-methoxyphenyl, 2-methoxy-5-fluorophenyl, 2-trifluoromethyl-5 fluorophenyl, 2-difluoromethyl-5-fluorophenyl, 3-chloro-5-fluorophenyl, 3-fluoro-5-(2 morpholinylethoxy) phenyl, 5-fluoro-2-(2-morpholinoethoxy)phenyl, 3-fluoro-5 methoxyethoxyphenyl or 5-fluoro-2-methoxyethoxyphenyl; X is CH 2 ; and n is 0. [00218] In certain embodiments of Formula Ib, R2 is H, (1-6C)alkyl, -(1 6C)hydroxyalkyl or -(2-6C)dihydroxyalkyl; Y is pyridyl optionally substituted with one or more substituents independently selected from F, OMe and Me; X is CH 2 ; and n is 0. [00219] In certain embodiments of Formula Ib, R 2 is H, methyl, ethyl, isopropyl, tert butyl, CH 2

CH

2 OH, or CH 2

CH(OH)CH

2 OH; Y is pyrid-2-yl, pyrid-3-yl, 5-fluoropyrid-3-yl, 2-methoxy-5-fluoropyridy-3-yl or 2-methyl-5-fluoropyridy-3-yl; X is CH 2 ; and n is 0. [00220] Compounds of Formula I also include compounds of Formula Ic, WO 2011/006074 PCT/US2010/041538 32

R

3 N 7-N N R1

(R

4 ) N x 0 R 2 Ic [00221] and salts thereof, wherein: [00222] NR 1

R

2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from (1-6C)alkyl, OH, CO 2 H and (1-3C alkyl)CO 2 H; [00223] X is CH 2 or CH 2

CH

2 ; [00224] Y is (i) phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl) or (ii) a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1 4C)alkyl; [002251 hetCyc 3 is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0; [00226] R 3 is H; [002271 each R4 is independently selected from halogen, -(1-4C)alkyl, -OH, -(1 4C)alkoxy, -NH 2 , -NH(1-4C alkyl) and -CH 2 OH; and [00228] nis0, 1,or2. [00229] In certain embodiments of Formula Ic, NR 1

R

2 forms 4-6 membered azacyclic ring optionally substituted with one or two groups independently selected from methyl, OH, C(=O)OH or CH 2 COOH; Y is phenyl optionally substituted with one or more substituents independently selected from -F, -Cl, -OMe, -CF 3 , -CHF 2 , morpholinylethoxy and OCH 2

CH

2 OMe; X is CH 2 ; and n is 0. [00230] In certain embodiments of Formula Ic, Y is phenyl, 3-fluorophenyl, 2,5 difluorophenyl, 2-chloro-5-fluorophenyl, 2-methoxyphenyl, 2-methoxy-5-fluorophenyl, 2 trifluoromethyl-5-fluorophenyl, 2-difluoromethyl-5-fluorophenyl, 3-chloro-5-fluorophenyl, 3-fluoro-5-(2-morpholinylethoxy) phenyl, 5-fluoro-2-(2-morpholinoethoxy)phenyl, 3-fluoro 5-methoxyethoxyphenyl or 5-fluoro-2-methoxyethoxyphenyl; X is CH 2 ; n is 0; and NR 1

R

2 forms 4-6 membered azacyclic ring selected from one of the following structures: WO 2011/006074 PCT/US2010/041538 33 N OH N OH N OH N CO2HN CO 2 H [002311 In certain embodiments of Formula Ic, NR 1

R

2 forms 4-6 membered azacyclic ring optionally substituted with one or two groups independently selected from methyl, OH, C(=O)OH or CH 2 COOH; Y is pyridyl optionally substituted with one or more substituents independently selected from F, OMe and Me; X is CH 2 ; and n is 0. [00232] In certain embodiments of Formula Ic, Y is pyrid-2-yl, pyrid-3-yl, 5 fluoropyrid-3-yl, 2-methoxy-5-fluoropyridy-3-yl or 2-methyl-5-fluoropyridy-3-yl; X is CH 2 ; n is 0; and NR 1

R

2 forms 4-6 membered azacyclic ring selected from one of the following structures: OH N OHN OH N CO2H N CO 2 H [00233] Compounds of Formula I also include compounds of Formula Id: -N R3 PN N N R1 (R 4)nT, N x 0 R 2 Id [00234] and salts thereof, wherein: [002351 R 1 is H; [00236] R 2 is Cyc' or a bridged 7-membered cycloalkyl ring, wherein Cyc' is a 3-6 membered cycloalkyl ring optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H and -(1-4C alkyl)OH; [002371 Y is (i) phenyl optionally substituted with one or more substituents independently selected from halogen, -(1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl) or (ii) a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1 4C)alkyl; WO 2011/006074 PCT/US2010/041538 34 [002381 hetCyc 3 is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0; [00239] X is CH 2 or CH 2

CH

2 ; [00240] R 3 is H; [00241] each R4 is independently selected from halogen, -(1-4C)alkyl, -OH, -(1 4C)alkoxy, -NH 2 , -NH(1-4C alkyl) and -CH 2 OH; and [00242] nis0, 1,or2. [00243] In certain embodiments of Formula Id, R 2 is Cyc' which is optionally substituted with one or more substituents independently selected from methyl, -OH, -CH 2 OH and -CO 2 H; Y is phenyl optionally substituted with one or more substituents independently selected from -F, -Cl, -OMe, -CF 3 , -CHF 2 , morpholinylethoxy and -OCH 2

CH

2 OMe; X is

CH

2 ; and n is 0. [00244] In certain embodiments of Formula Id, Y is phenyl, 3-fluorophenyl, 2,5 difluorophenyl, 2-chloro-5-fluorophenyl, 2-methoxyphenyl, 2-methoxy-5-fluorophenyl, 2 trifluoromethyl-5-fluorophenyl, 2-difluoromethyl-5-fluorophenyl, 3-chloro-5-fluorophenyl, 3-fluoro-5-(2-morpholinylethoxy)phenyl, 5-fluoro-2-(2-morpholinoethoxy)phenyl, 3-fluoro 5-methoxyethoxyphenyl or 5-fluoro-2-methoxyethoxyphenyl; X is CH 2 , n is 0; and R 2 is selected from the structures: OH 0 2 H OH

CO

2 H OH HO [002451 In certain embodiments of Formula Id, R 2 is cyclopropyl optionally substituted with one or two substituents independently selected from methyl, -OH, -CH 2 OH and -CO 2 H; Y is phenyl optionally substituted with one or more substituents independently selected from -F, -Cl, -OMe, -CF 3 , -CHF 2 , morpholinylethoxy and -OCH 2

CH

2 OMe; X is

CH

2 ; and n is 0. [00246] In certain embodiments of Formula Id, R 2 is Cyc' which is optionally substituted with one or more substituents independently selected from methyl, -OH, -CH 2 OH and -CO 2 H; Y is pyridyl optionally substituted with one or more substituents independently selected from F, OMe and Me; X is CH 2 ; and n is 0.

WO 2011/006074 PCT/US2010/041538 35 [002471 In certain embodiments of Formula Id, Y is pyrid-2-yl, pyrid-3-yl, 5 fluoropyrid-3-yl, 2-methoxy-5-fluoropyridy-3-yl or 2-methyl-5-fluoropyridy-3-yl; X is CH 2 ; n is 0; and R 2 is selected from the structures: OH C0 2 H OH

CO

2 H OH HO [00248] Compounds of Formula I also include compounds of Formula le

R

3 N N N NR1

(R

4 ). N x 0 R 2 le [00249] and salts thereof, wherein: [002501 R 1 is H; [002511 R 2 is -(1-6C alkyl)CN, -(1-6C alkyl)SO 2

NH

2 , -(1-6C alkyl)NHSO 2 (1-3C alkyl), -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl), or -(1-6C alkyl)N(1-4C alkyl) 2 ; [00252] Y is (i) phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl), or (ii) a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen and -O(1-4C alkyl); [00253] hetCyc 3 is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0; [00254] X is CH 2 or CH 2

CH

2 ; [002551 R 3 is H; [00256] each R4 is independently selected from halogen, -(1-4C)alkyl, -OH, -(1 4C)alkoxy, -NH 2 , -NH(1-4C alkyl) and -CH 2 OH; and [002571 nis0, 1,or2.

WO 2011/006074 PCT/US2010/041538 36 [002581 In certain embodiments of Formula le, Y is phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3

-CHF

2 , O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl); X is CH 2 ; and n is 0. [002591 In certain embodiments of Formula le, Y is phenyl, 3-fluorophenyl, 2,5 difluorophenyl, 2-chloro-5-fluorophenyl, 2-methoxyphenyl, 2-methoxy-5-fluorophenyl, 2 trifluoromethyl-5-fluorophenyl, 2-difluoromethyl-5-fluorophenyl, 3-chloro-5-fluorophenyl, 3-fluoro-5-(2-morpholinylethoxy) phenyl, 5-fluoro-2-(2-morpholinoethoxy)phenyl, 3-fluoro 5-methoxyethoxyphenyl or 5-fluoro-2-methoxyethoxyphenyl; X is CH 2 ; and n is 0. [00260] In certain embodiments of Formula le, Y is a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen and -O(1-4C alkyl); X is CH 2 ; and n is 0. [00261] In certain embodiments of Formula le, Y is pyrid-2-yl, pyrid-3-yl, 5 fluoropyrid-3-yl, 2-methoxy-5-fluoropyridy-3-yl or 2-methyl-5-fluoropyridy-3-yl; X is CH 2 and n is 0. [00262] Compounds of Formula I also include compounds of Formula If R 3 N >LN N R1

(R

4 ) X 0 R2 If [00263] and salts thereof, wherein: [00264] R 1 is H; [002651 R2 is -(1-6C alkyl)hetCyc', -(1-6C alkyl)hetArl, hetAr 2 or hetCyc 2 ; [00266] hetCyc' is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc' is optionally substituted with oxo; [002671 hetCyc 2 is a 6 membered carbon-linked heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc 2 is optionally substituted with F, SO 2

NH

2 , or S0 2 (1-3C alkyl); [00268] hetArl is a 5-membered heteroaryl ring having 1-2 ring heteroatoms independently selected from N and 0 and optionally substituted with (1-4C)alkyl; [00269] hetAr 2 is a 5-6 membered heteroaryl ring having 1-2 ring nitrogen atoms and optionally substituted with one or more substituents independently selected from (1 -4C)alkyl; WO 2011/006074 PCT/US2010/041538 37 [002701 Y is (i) phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl), or (ii) a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen and -O(1-4C alkyl); [002711 X is CH 2 or CH 2

CH

2 ; [00272] R 3 is H; [00273] each R4 is independently selected from halogen, -(1-4C)alkyl, -OH, -(1 4C)alkoxy, -NH 2 , -NH(1-4C alkyl) and -CH 2 OH; and [00274] nis0, 1,or2. [002751 In certain embodiments of Formula If, R 2 is -(1-6C alkyl)hetArl or hetAr 2 ; y is (i) phenyl optionally substituted with one or more substituents independently selected from halogen, (1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl); X is CH 2 ; and n is 0. [00276] In certain embodiments of Formula If, R 2 is -(1-6C alkyl)hetArl or hetAr 2 hetArl is a furanyl, pyrazolyl, or imidazolyl ring optionally substituted with -(1-4C alkyl); hetAr2 is a pyridyl or pyrazolo ring optionally substituted with one or more methyl groups; Y is phenyl, 3-fluorophenyl, 2,5-difluorophenyl, 2-methoxyphenyl, 2-chloro-5-fluorophenyl, 2 methoxy-5-fluorophenyl, 2-trifluoromethyl-5-fluorophenyl, 2-difluoromethyl-5-fluorophenyl, 3-chloro-5-fluorophenyl, 3-fluoro-5-(2-morpholinylethoxy)phenyl, 5-fluoro-2-(2 morpholinoethoxy)phenyl, 3-fluoro-5-methoxyethoxyphenyl or 5-fluoro-2 methoxyethoxyphenyl; X is CH 2 and n is 0. [002771 In certain embodiments of Formula If, R 2 is -(1 -6C alkyl)hetArl or hetAr 2 ; y is a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen and -O(1-4C alkyl); X is CH 2 ; and n is 0. [00278] In certain embodiments of Formula If, R 2 is -(1-6C alkyl)hetArl or hetAr 2 hetArl is a furanyl, pyrazolyl, or imidazolyl ring optionally substituted with -(1-4C alkyl); hetAr2 is a pyridyl or pyrazolo ring optionally substituted with one or more methyl groups; Y is pyrid-2-yl, pyrid-3-yl, 5-fluoropyrid-3-yl, 2-methoxy-5-fluoropyridy-3-yl or 2-methyl 5-fluoropyridy-3-yl; X is CH 2 ; and n is 0. [002791 In certain embodiments of Formula If, R 2 is -(1-6C alkyl)hetCyc' or hetCyc 2 ; Y is (i) phenyl optionally substituted with one or more substituents independently selected WO 2011/006074 PCT/US2010/041538 38 from halogen, (1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl); X is CH 2 ; and n is 0. [002801 In certain embodiments of Formula If, R 2 is -(1-6C alkyl)hetCyc' or hetCyc 2 ; hetCyc' is a morpholinyl or imidazolidin-2-one ring; hetCyc 2 is a piperidinyl or tetrahydropyranyl ring optionally substituted with F, SO 2

NH

2 , or S0 2 (1-3C alkyl); Y is phenyl, 3-fluorophenyl, 2,5-difluorophenyl, 2-chloro-5-fluorophenyl, 2-methoxyphenyl, 2 methoxy-5-fluorophenyl, 2-trifluoromethyl-5-fluorophenyl, 2-difluoromethyl-5-fluorophenyl, 3-chloro-5-fluorophenyl, 3-fluoro-5-(2-morpholinylethoxy)phenyl, 5-fluoro-2-(2 morpholinoethoxy)phenyl, 3-fluoro-5-methoxyethoxyphenyl or 5-fluoro-2 methoxyethoxyphenyl; X is CH 2 ; and n is 0. [002811 In certain embodiments of Formula If, R 2 is hetCyc 2 ; hetCyc 2 is a piperidinyl or tetrahydropyranyl ring optionally substituted with F, SO 2

NH

2 , or S0 2 (1-3C alkyl); Y is pyrid-2-yl, pyrid-3-yl, 5-fluoropyrid-3-yl, 2-methoxy-5-fluoropyridy-3-yl or 2-methyl-5 fluoropyridy-3-yl; X is CH 2 ; and n is 0. [00282] The compounds of Formula I also include compounds of Formula Ig R 3 O-NN N NR1 (R )n ) N x 0 R 2 Ig [00283] and salts thereof, wherein: [00284] R 1 is H; [002851 R 2 is -O(1-6C alkyl), -O(3-6C cycloalkyl); [002861 Y is (i) phenyl optionally substituted with one or more substituents independently selected from halogen, -(1-4C)alkoxy, -CF 3

-CHF

2 , -O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl) or (ii) a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1-4C)alkyl; [002871 hetCyc 3 is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0; [002881 X is CH 2 or CH 2

CH

2 ; [002891 R3 is H; [00290] each R4 is independently selected from halogen, -(1-4C)alkyl, -OH, -(1 4C)alkoxy, -NH 2 , -NH(1-4C alkyl) and -CH 2 OH; and WO 2011/006074 PCT/US2010/041538 39 [00291] nis0, 1,or2. [00292] In certain embodiments of Formula Ig, R 2 is -O(1-6C alkyl), -O(3-6C cycloalkyl); Y is phenyl optionally substituted with one or more substituents independently selected from -F, -Cl, -OMe, -CF 3 , -CHF 2 , morpholinylethoxy and -OCH 2

CH

2 OMe; X is

CH

2 and n is 0. [002931 In certain embodiments of Formula Ig, R2 is OMe, OEt or cyclopropoxy; Y is phenyl, 3-fluorophenyl, 2,5-difluorophenyl, 2-chloro-5-fluorophenyl, 2-methoxyphenyl, 2 methoxy-5-fluorophenyl, 2-trifluoromethyl-5-fluorophenyl, 2-difluoromethyl-5-fluorophenyl, 3-chloro-5-fluorophenyl, 3-fluoro-5-(2-morpholinylethoxy)phenyl, 5-fluoro-2-(2 morpholinoethoxy)phenyl, 3-fluoro-5-methoxyethoxyphenyl or 5-fluoro-2 methoxyethoxyphenyl; X is CH 2 ; and n is 0. [00294] In certain embodiments of Formula Ig, R 2 is -O(1-6C alkyl), -O(3-6C cycloalkyl); Y is pyridyl optionally substituted with one or more substituents independently selected from F, OMe and Me; X is CH 2 ; and n is 0. [002951 In certain embodiments of Formula Ig, R 2 is OMe, OEt or cyclopropoxy; Y is pyrid-2-yl, pyrid-3-yl, 5-fluoropyrid-3-yl, 2-methoxy-5-fluoropyridy-3-yl or 2-methyl-5 fluoropyridy-3-yl; X is CH 2 ; and n is 0. [00296] The compounds of Formula I also include compounds of Formula Ih R3 pN N NR1 (R 4 )n N x 0 R 2 Ih [002971 and salts thereof, wherein: [00298] R 1 is H or -(1-6C alkyl); [00299] R2 is H, -(1-6C)alkyl, -(1-6C)fluoroalkyl, -(1-6C)hydroxyalkyl, -(2 6C)dihydroxyalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2

NH

2 , -(1-6C alkyl)NHSO 2 (1-3C alkyl), -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl), -(1-6C alkyl)N(1-4C alkyl) 2 , -(1 6C alkyl)hetCyc', -(1-6C alkyl)hetArl, hetAr 2 , hetCyc 2 , -O(1-6C alkyl), -O(3-6C cycloalkyl), or Cyc'; [003001 or NR R2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from -(1-6C)alkyl, -OH, -CO 2 H and -(1-3C alkyl)CO 2

H;

WO 2011/006074 PCT/US2010/041538 40 [00301] Cyc' is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, OMe, -CO 2 H and -(1-4C alkyl)OH; [00302] hetCyc' is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc' is optionally substituted with oxo; [003031 hetCyc 2 is a 6 membered carbon-linked heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc 2 is optionally substituted with F, SO 2

NH

2 , or SO 2 (1-3C alkyl); [00304] hetArl is a 5-membered heteroaryl ring having 1-2 ring heteroatoms independently selected from N and 0 and optionally substituted with (1-4C)alkyl; [003051 hetAr 2 is a 5-6 membered heteroaryl ring having 1-2 ring nitrogen atoms and optionally substituted with one or more substituents independently selected from (1 -4C)alkyl; [00306] X is CH 2 ; [003071 Y is (i) fluorophenyl optionally substituted with a substituent selected from O(1-4C alkyl)hetCyc 3 , -(1-4C alkyl)hetCyc 3 , -O(1-4C alkyl)O(1-3C alkyl) and -O(3-6C dihydroxyalkyl), (ii) pyridyl substituted with one or more substituents independently selected from F, methyl and ethyl, or (iii) 5-fluoropyridin-2(1H)-one optionally substituted with (1 4C)alkyl; [003081 R 3 is H or -(1-4C alkyl); [003091 each R4 is independently selected from halogen, -(1-4C)alkyl, -OH, -(1 4C)alkoxy, -NH 2 , -NH(1-4C alkyl) and -CH 2 OH; and [003101 nis0, 1, 2, 3, 4,5 or6. [003111 In one embodiment of Formula Ih, Y is fluorophenyl optionally substituted with a substituent selected from -O(1-4C alkyl)hetCyc 3 , -(1-4C alkyl)hetCyc 3 , -O(1-4C alkyl)O(1-3C alkyl) and -0(3-6C dihydroxyalkyl). [00312] In one embodiment of Formula Ih, Y is pyridyl substituted with one or more substituents independently selected from F, methyl and ethyl. [003131 In one embodiment of Formula Ih, Y is 5-fluoropyridin-2(1H)-one optionally substituted with (1-4C)alkyl. [00314] In one embodiment of Formula Ih, R 2 is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from (1-4C alkyl), -OH, OMe, -CO 2 H and -(1-4C alkyl)OH.

WO 2011/006074 PCT/US2010/041538 41 [003151 In one embodiment of Formula Ih, R 2 is cyclopropyl optionally substituted with methyl, -CO 2 H or -CH 2 OH. [00316] In one embodiment of Formula Ih, R4 is OH, F, methyl, or CH 2 OH. [00317] In one embodiment of Formula Ih, n is 0, 1 or 2. [003181 In one embodiment of Formula Ih, R3 is hydrogen. [003191 In one embodiment of Formula Ih, R1 is H; R2 is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, OMe, -CO 2 H and -(1-4C alkyl)OH; X is CH 2 ; Y is (i) fluorophenyl optionally substituted with a substituent selected from -O(1-4C alkyl)hetCyc 3 , O(1-4C alkyl)O(1-3C alkyl) and -O(3-6C dihydroxyalkyl), (ii) pyridyl substituted with one or more substituents independently selected from F, methyl and ethyl, or (iii) 5 fluoropyridin-2(1H)-one optionally substituted with (1-4C)alkyl; R3 is H, and n is 0. [00320] It will be appreciated that certain compounds of the invention may contain asymmetric or chiral centers, and therefore exist in different stereoisomeric forms. It is intended that all stereoisomeric forms of the compounds of the invention, including but not limited to, diastereomers, enantiomers and atropisomers, as well as mixtures thereof such as racemic mixtures, form part of the present invention. [00321] In the structures shown herein, where the stereochemistry of any particular chiral atom is not specified, then all stereoisomers are contemplated and included as the compounds of the invention. Where stereochemistry is specified by a solid wedge or dashed line representing a particular configuration, then that stereoisomer is so specified and defined. [00322] It will also be appreciated that certain compounds of Formula I may be used as intermediates for further compounds of Formula I. [003231 The compounds of Formula I include salts thereof. In certain embodiments, the salts are pharmaceutically acceptable salts. In addition, the compounds of Formula I include other salts of such compounds which are not necessarily pharmaceutically acceptable salts, and which may be useful as intermediates for preparing and/or purifying compounds of Formula I and/or for separating enantiomers of compounds of Formula I. [00324] It will further be appreciated that the compounds of Formula I and their salts may be isolated in the form of solvates, and accordingly that any such solvate is included within the scope of the present invention. [003251 Compounds of the invention may also contain unnatural proportions of atomic isotopes at one or more of the atoms that constitute such compounds. That is, an atom, in WO 2011/006074 PCT/US2010/041538 42 particular when mentioned in relation to a compound according to Formula I, comprises all isotopes and isotopic mixtures of that atom, either naturally occurring or synthetically produced, either with natural abundance or in an isotopically enriched form. For example, when hydrogen is mentioned, it is understood to refer to H2H HH or mixtures thereof carbon is mentioned, it is understood to refer to HC, 1C, HC, C or mixtures thereof; when nitrogen is mentioned, it is understood to refer to 1N, "N, N or mixtures thereof; when oxygen is mentioned, it is understood to refer to O, O, 1 0 or mixtures there heof and when fluoro is mentioned, it is understood to refer to 1 8 F, 1F or mixtures thereof. The compounds according to the invention therefore also comprise compounds with one or more isotopes of one or more atom, and mixtures thereof, including radioactive compounds, wherein one or more non-radioactive atoms has been replaced by one of its radioactive enriched isotopes. Radiolabeled compounds are useful as therapeutics, research reagents, e.g., assay reagents, and diagnostic agents, e.g., in vivo imaging agents. All isotopic variations of the compounds of the present invention, whether radioactive or not, are intended to be encompassed within the scope of the present invention. [00326] The term "(1-6C) alkyl" as used herein refers to saturated linear or branched chain monovalent hydrocarbon radicals of one to six carbon atoms, respectively. Examples include, but are not limited to, methyl, ethyl, 1-propyl, 2-propyl, 1-butyl, 2-methyl-1-propyl, 2-butyl, 2-methyl-2-propyl, pentyl, and hexyl. The definition of "(1-6C) alkyl" likewise applies to the term "O-(1-6C alkyl)". [003271 The terms "(1-6C)fluoroalkyl", "(1-6C alkyl)CN", "(1-6C alkyl)SO 2 NH2", "(1 6C alkyl)NHSO 2 (1-3C alkyl)", "(1-6C alkyl)NH 2 ", "(1-6C alkyl)NH(1-4C alkyl)", "(1-6C alkyl)N(1-4C alkyl)2", "(1-6C alkyl)hetCyc" and "(1-6C alkyl)hetAr" as used herein refer to saturated linear or branched-chain monovalent hydrocarbon radicals of one to six carbon atoms, respectively, wherein one of the hydrogen atoms is replaced with a fluoro atom, or a CN, SO 2

NH

2 , NHSO 2 (1-3C alkyl), NH 2 , NH(1-4C alkyl), N(1-4C alkyl) 2 , hetCyc or hetAr group, respectively. [003281 The term "(1-6C)chloroalkyl" as used herein refers to saturated linear or branched-chain monovalent hydrocarbon radicals of one to six carbon atoms, respectively, wherein one of the hydrogen atoms is replaced with a chloro atom. [00329] The term "(1-6C)hydroxyalkyl" as used herein refers to saturated linear or branched-chain monovalent hydrocarbon radicals of one to six carbon atoms, respectively, wherein one of the hydrogen atoms are replaced with a OH group.

WO 2011/006074 PCT/US2010/041538 43 [003301 The term "(2-6C)dihydroxyalkyl" as used herein refers to saturated linear or branched-chain monovalent hydrocarbon radicals of two to six carbon atoms, respectively, wherein two of the hydrogen atoms are replaced with a OH group, provided that two OH groups are not on the same carbon. [003311 The term "(1-6C)difluoroalkyl" as used herein refers to saturated linear or branched-chain monovalent hydrocarbon radicals of one to six carbon atoms, respectively, wherein two of the hydrogen atoms are replaced with a fluoro atom. [00332] The term "(1-6C)trifluoroalkyl" as used herein refers to saturated linear or branched-chain monovalent hydrocarbon radicals of one to six carbon atoms, respectively, wherein three of the hydrogen atoms are replaced with a fluoro atom. [003331 The term "(2-6C)chlorofluoroalkyl" as used herein refers to saturated linear or branched-chain monovalent hydrocarbon radicals of two to six carbon atoms, respectively, wherein one of the hydrogen atoms is replaced with a chloro atom and one of the hydrogen atoms is replaced with a fluoro atom. [00334] The term "(2-6C)difluorochloroalkyl" as used herein refers to saturated linear or branched-chain monovalent hydrocarbon radicals of two to six carbon atoms, respectively, wherein one of the hydrogen atoms is replaced with a chloro and two of the hydrogen atoms are replaced with a fluoro atom. [003351 The term "(2-6C)chlorohydroxyalkyl" as used herein refers to a saturated linear or branched-chain monovalent hydrocarbon radicals of two to six carbon atoms, respectively, wherein one of the hydrogen atoms is replaced with a chloro and one of the hydrogen atoms is replaced with OH. [003361 The term "(1-6C alkyl)NHC(=0)O(1-4C alkyl)" as used herein refers to saturated linear or branched-chain monovalent hydrocarbon radicals of one to six carbon atoms, respectively, wherein one of the hydrogen atoms is replaced with a -NHC(=0)O(1-4C alkyl) group. [003371 The phrase "O(1-6C alkyl) which is optionally substituted with halogen, OH or (1-4C)alkoxy" as used herein refers to a saturated linear or branched-chain monovalent alkyl ether radical of one to six carbon atoms wherein the term "alkyl" is as defined herein and the radical is on the oxygen atom, and one of the hydrogen atoms on the carbon chain is optionally replaced with halogen, OH or (1 -4C)alkoxy. Examples include methoxy, ethoxy, propoxy, isopropoxy, and butoxy radicals optionally substituted with halogen, OH or (1 4C)alkoxy.

WO 2011/006074 PCT/US2010/041538 44 [003381 The term "O(3-6C cycloalkyl)" as used herein refers to a cycloalkyl ether radical wherein the term "cycloalkyl" is a a 3-6 membered carbocyclic ring and the radical is on the oxygen atom. [003391 The term "-(1-6C alkyl)(3-6C cycloalkyl)" as used herein refers to a saturated linear or branched-chain monovalent hydrocarbon radical of one to six carbon atoms, respectively, wherein one of the hydrogen atoms is replaced with a 3-6 membered carbocyclic ring. [00340] The term "-(1-6Calkyl)(1-4C alkoxy)" as used herein refers to a saturated linear or branched-chain monovalent hydrocarbon radical of one to six carbon atoms, respectively, wherein one of the hydrogen atoms is replaced with an (1-4C)alkoxy group. [00341] The term "-(1-6C hydroxyalkyl)(1-4C alkoxy)" as used herein refers to a saturated linear or branched-chain monovalent hydrocarbon radical of one to six carbon atoms, respectively, wherein one of the hydrogen atoms is replaced with hydroxy (OH) group and one of the hydrogen atoms is replaced with an (1-4C)alkoxy group. [00342] The term "halogen" includes fluoro, chloro, bromo and iodo. [003431 The term "pharmaceutically acceptable" indicates that the substance or composition is compatible chemically and/or toxicologically, with the other ingredients comprising a formulation, and/or the mammal being treated therewith. [00344] The present invention also provides a process for the preparation of a compound of Formula I or a salt thereof as defined herein, which comprises: [003451 (a) reacting a corresponding compound of Formula II

R

3 N N N

(R

4 )n OH x O II 12 [003461 or a reactive derivative thereof with an amine having the formula HNR R2; or [003471 (b) for compounds of Formula I where R 1 and R 2 are each hydrogen, reacting a compound of Formula III N 4 NN R 3 (R 4 )n- C N x

III

WO 2011/006074 PCT/US2010/041538 45 [00348] with an inorganic acid; or [00349] (c) for a compound of Formula I where R 2 is (alkyl)NHSO 2 ((1-3C alkyl), reacting a compound having the formula IV

R

3 N N N N (R4)--- )NH(1 -6C alkyl)NH 2 x o IV [003501 with -(1-3C alkyl)SO 2 Cl; or [003511 (d) for compounds of Formula I wherein Y is 5-fluoropyridin-2(1H)-one, treating a corresponding compound having the formula VIII F

R

3 N N N R1 '2 N S0 R 2 (R 4 ) VIII [00352] with an acid at elevated temperatures; or [00353] (e) for a compound of Formula I wherein R 2 is CH 2

CH(OH)CH

2 OH, treating a corresponding compound having the formula IX -N R 3 ON N (R 4 )nI N N R1 x00 IX [00354] with an acid; or [003551 (f) for a compound of Formula I wherein Y is fluorophenyl substituted with OCH 2

CH(OH)CH

2 OH, treating a corresponding compound having the formula X F O-- R3 N/R1 IR 0 0N 0 74)/ 0 N WO 2011/006074 PCT/US2010/041538 46 X [003561 with an acid; and [00357] removing or adding any protecting groups if desired, and forming a salt if desired. [00358] Referring to method (a), the coupling of the compound of formula II with an amine having the formula HNR 1

R

2 may be performed using conventional amide bond formation conditions, for example by reacting an amine with a reactive derivative of a carboxylic acid, for example an acid halide, such as an acid chloride. When reacting the acid form of a compound of Formula II, the reaction may be performed in the presence of a suitable coupling agent such as 2-(1H-7-Azabenzotriazol-1-yl)-1,1,3,3-tetramethyl uronium hexafluorophosphate methanaminium (HATU), O-Benzotriazole-N,N,N',N'-tetramethyl uronium-hexafluoro-phosphate (HBTU), 0-(Benzotriazol- 1 -yl)-N,N,N',N'-tetramethyl uronium tetrafluoroborate (TBTU), N,N'-dicyclohexylcarbodiimide (DCC), 1-(3 dimethylaminopropyl)-3-ethylcarboiimide (DIEC) and any other amide coupling reagents well known to persons skilled in the art. Suitable bases include tertiary amine bases such as diisopropylethylamine (DIlEA) and triethylamine. Suitable solvents include DMF and

CH

3 CN. [003591 Referring to method (b), suitable acids include strong inorganic acids such as sulfuric acid. [00360] Referring to methods (d), (e) and (f), suitable acids include inorganic acids such as hydrogen halides, for example HCl. [00361] Compounds of formula II may be prepared by coupling a corresponding compound having formula IV

R

3 N H

(R

4 )FN) x IV [00362] with a corresponding compound having formula V N

Z

1 N Opi 0

V

WO 2011/006074 PCT/US2010/041538 47 [003631 where Z' is OH or a leaving group or atom and P 1 is H or a carboxyl protecting group. The leaving atom represented by Z' may be, for example, a halogen atom such as a chlorine atom. In this instance, the reaction is performed in the presence of a base, such as an amine base, for example diisopropylethylamine. The reaction is conveniently performed at elevated temperatures, for example at 100 'C. Convenient solvents include alcohols such as butanol. When Z' is OH the reaction is performed in the presence of a coupling reagent. Suitable coupling reagents when Z' is OH include benzotriazolyloxy tris [dimethyl-amino] phosphonium hexafluorophosphate (BOP), HATU, HBTU or TBTU. The carboxyl protecting group may be any convenient carboxyl protecting group, for example as described in Greene & Wuts, eds., "Protecting Groups in Organic Synthesis", John Wiley & Sons, Inc. Examples of carboxyl protecting groups include (1-6C)alkyl groups, such as methyl, ethyl and t-butyl. [00364] A compound of Formula V can be prepared by cyclizing a corresponding compound of Formula VI H N N 1 /

H

2 N op 1 0 VI [003651 with (E)-ethyl 3-ethoxyacrylate to provide the compound of Formula V where Z' is OH as shown 01N N HO N

OP

1 0 V [00366] or when Z' is a leaving group or atom, converting the hydroxy group into a leaving atom or group, for example by treating the compound of Formula V where Z 1 is OH with POCl 3 . [003671 Compounds of Formula I where the Y group has the absolute configuration shown in Figure Ia: WO 2011/006074 PCT/US2010/041538 48 f-N- N R3N N N R1 x 0 R Ia [00368] are prepared by coupling a compound of Formula V with a corresponding compound having the formula IV-A

R

3 NH

(R

4 )F ) X. IV-A [00369] The compound of Formula IV-A can be prepared by treating a compound of Formula VII

P

2 (4k (R 4)./-N) x VII [0001] where P2 is an amine protecting group, with an alkyl lithium base (for example sec-butyl lithium) in the presence of a chiral complexing agent (for example (-)-sparteine), followed by coupling with a compound having Y-Z 2 where Z 2 is a leaving group or atom, such as a halogen atom (for example bromine) in the presence of a palladium (II) catalyst and a ligand. Such enantioselective palladium-catalyzed reactions are described in Campos, et al., J. Am. Chem. Soc., 2006, 128:3538-3539. Suitable catalysts include Pd(OAc) 2 . Suitable ligands include phosphine ligands such as t-Bu 3

P-HBF

4 . The amine protecting group may be any convenient amine protecting group, for example as described in Greene & Wuts, eds., "Protecting Groups in Organic Synthesis", John Wiley & Sons, Inc. Examples of amine protecting groups include acyl and alkoxycarbonyl groups, such as t-butoxycarbonyl (BOC). [0001] The compounds of the formulas II, III, and IV are also believed to be novel and are provided as further aspects of the invention. [003701 The ability of compounds of the invention to act as TrkA inhibitors may be demonstrated by the assays described in Examples A and B. The ability of compounds of the invention to act as TrkB inhibitors may be demonstrated by the assay described in Example

B.

WO 2011/006074 PCT/US2010/041538 49 [003711 The selectivity of compounds of Formula I for TrkA versus one or more JAK kinases was determined using the assays describes in Examples C, D, E and F. [00372] It was unexpectedly discovered that compounds of Formula I wherein X is

CH

2 are particularly selective for inhibiting TrkA activity over inhibiting the activity of one or more JAK kinases, for example JAK2, as shown in Table 1. In one embodiment, compounds of Formula I are 10-30 fold more potent in inhibiting TrkA kinase activity over inhibiting Jak2 kinase activity. In one embodiment, compounds of Formula I are 30-100 fold more potent in inhibiting TrkA kinase activity over inhibiting Jak2 kinase activity. In one embodiment, compounds of Formula I are greater than 100 fold more potent in inhibiting TrkA kinase activity over inhibiting Jak2 kinase activity. [00373] Additionally, it was unexpectedly discovered that compounds of Formula I wherein R2 is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, OMe, -CO 2 H and -(1 4C alkyl)OH are particularly selective for inhibiting TrkA activity over inhibiting the activity of one or more JAK kinases, for example JAK2, as shown in Table 1. [00374] Additionally, it was unexpectedly discovered that compounds of Formula I wherein Y is (i) fluorophenyl optionally substituted with a substituent selected from -0(1-4C alkyl)hetCyc 3 , -O(1-4C alkyl)O(1-3C alkyl) and -O(3-6C dihydroxyalkyl), (ii) pyridyl substituted with one or more substituents independently selected from F, methyl and ethyl, or (iii) 5-fluoropyridin-2(1H)-one optionally substituted with (1-4C)alkyl, are particularly selective for inhibiting TrkA activity over inhibiting the activity of one or more JAK kinases, for example JAK2, as shown in Table 1. [003751 Compounds of Formula I are useful for treating pain, including chronic and acute pain. Certain compounds which are inhibitors of TrkA and/or TrkB may be useful in the treatment of multiple types of pain including inflammatory pain, neuropathic pain, and pain associated with cancer, surgery, and bone fracture. [00376] In one embodiment, compounds of Formula I are useful for treating acute pain. Acute pain, as defined by the International Association for the Study of Pain, results from disease, inflammation, or injury to tissues. This type of pain generally comes on suddenly, for example, after trauma or surgery, and may be accompanied by anxiety or stress. The cause can usually be diagnosed and treated, and the pain is confined to a given period of time and severity. In some instances, it can become chronic.

WO 2011/006074 PCT/US2010/041538 50 [003771 In one embodiment, compounds of Formula I are useful for treating chronic pain. Chronic pain, as defined by the International Association for the Study of Pain, is widely believed to represent disease itself. It can be made much worse by environmental and psychological factors. Chronic pain persists over a longer period than acute pain and is resistant to most medical treatments, generally over 3 months or more. It can and often does cause severe problems for patients. [00378] Compounds of Formula I are also useful for treating cancer. Particular examples include neuroblastoma, ovarian, pancreatic, colorectal and prostate cancer. [003791 Compounds of Formula I are also useful for treating inflammation and certain infectious diseases. [00380] In addition, compounds of Formula I may also be used to treat interstitial cystitis (IC), painful bladder syndrome (PBS), urinary incontinence, asthma, anorexia, atopic dermatitis, and psoriasis. [00381] Compounds of Formula I are also useful for treating a neurodegenerative disease in a mammal, comprising administering to said mammal one or more compounds of Formula I or a pharmaceutically acceptable salt thereof in an amount effective to treat or prevent said neurodegenerative disease. In one embodiment, compounds of Formula I may also be used to treat demyelination and dysmyelination by promoting myelination, neuronal survival, and oligodendrocyte differentiation via blocking Sp35-TrkA interaction. In one embodiment, the neurodegenerative disease is multiple sclerosis. In one embodiment, the neurodegenerative disease is Parkinson's disease. In one embodiment, the neurodegenerative disease is Alzheimer's disease. [00382] As used herein, the term treatment includes prophylaxis as well as treatment of a preexisting condition. Beneficial or desired clinical results include, but are not limited to, alleviation of symptoms, diminishment of extent of disease, stabilized (i.e., not worsening) state of disease, delay or slowing of disease progression, amelioration or palliation of the disease state, and remission (whether partial or total), whether detectable or undetectable. "Treatment" can also mean prolonging survival as compared to expected survival if not receiving treatment. Those in need of treatment include those already with the condition or disorder, as well as those prone to have the condition or disorder or those in which the condition or disorder is to be prevented. [00383] Accordingly, another embodiment of this invention provides a method of treating pain in a mammal, comprising administering to said mammal one or more WO 2011/006074 PCT/US2010/041538 51 compounds of Formula I or a pharmaceutically acceptable salt thereof in an amount effective to treat or prevent said pain. [00384] Another embodiment of this invention provides a method of treating inflammation in a mammal, comprising administering to said mammal one or more compounds of Formula I or a pharmaceutically acceptable salt thereof in an amount effective to treat or prevent said inflammation. [003851 Another embodiment of this invention provides a method of treating a neurodegenerative disease in a mammal, comprising administering to said mammal one or more compounds of Formula I or a pharmaceutically acceptable salt thereof in an amount effective to treat or prevent said neurodegenerative disease. [00386] Another embodiment of this invention provides a method of treating Trypanosoma cruzi infection in a mammal, comprising administering to said mammal one or more compounds of Formula I or a pharmaceutically acceptable salt thereof in an amount effective to treat or prevent said Trypanosoma cruzi infection. [003871 The phrase "effective amount" means an amount of compound that, when administered to a mammal in need of such treatment, is sufficient to (i) treat or prevent a particular disease, condition, or disorder which can be treated with an inhibitor of TrkA and/or TrkB, (ii) attenuate, ameliorate, or eliminate one or more symptoms of the particular disease, condition, or disorder, or (iii) prevent or delay the onset of one or more symptoms of the particular disease, condition, or disorder described herein. [00388] The amount of a compound of Formula I that will correspond to such an amount will vary depending upon factors such as the particular compound, disease condition and its severity, the identity (e.g., weight) of the mammal in need of treatment, but can nevertheless be routinely determined by one skilled in the art. [00389] As used herein, the term "mammal" refers to a warm-blooded animal that has or is at risk of developing a disease described herein and includes, but is not limited to, guinea pigs, dogs, cats, rats, mice, hamsters, and primates, including humans. [00390] The compounds of the present invention can be used in combination with one or more additional drugs that work by the same or a different mechanism of action. Examples include anti-inflammatory compounds, steroids (e.g., dexamethasone, cortisone and fluticasone), analgesics such as NSAIDs (e.g., aspirin, ibuprofen, indomethacin, and ketoprofen), and opioids (such as morphine), and chemotherapeutic agents.

WO 2011/006074 PCT/US2010/041538 52 [00391] Compounds of the invention may be administered by any convenient route, e.g. into the gastrointestinal tract (e.g. rectally or orally), the nose, lungs, musculature or vasculature, or transdermally or dermally. Compounds may be administered in any convenient administrative form, e.g. tablets, powders, capsules, solutions, dispersions, suspensions, syrups, sprays, suppositories, gels, emulsions, patches etc. Such compositions may contain components conventional in pharmaceutical preparations, e.g. diluents, carriers, pH modifiers, sweeteners, bulking agents, and further active agents. If parenteral administration is desired, the compositions will be sterile and in a solution or suspension form suitable for injection or infusion. Such compositions form a further aspect of the invention. [00392] According to another aspect, the present invention provides a pharmaceutical composition, which comprises a compound of Formula I or a pharmaceutically acceptable salt thereof, as defined hereinabove, together with a pharmaceutically acceptable diluent or carrier. [00393] According to another embodiment, the present invention provides a compound of Formula I or a pharmaceutically acceptable salt thereof, for use in the treatment of pain in a mammal. [00394] In another embodiment, the present invention provides a compound of Formula I or a pharmaceutically acceptable salt thereof, for use in the treatment of inflammation in a mammal. [003951 In another embodiment, the present invention provides a compound of Formula I or a pharmaceutically acceptable salt thereof, for use in the treatment of infectious diseases, for example Trypanosoma cruzi infection, in a mammal. [00396] In another embodiment, the present invention provides a compound of Formula I or a pharmaceutically acceptable salt thereof, for use in the treatment of a neurodegenerative disease in a mammal. [003971 According to a further aspect, the present invention provides the use of a compound of Formula I or a pharmaceutically acceptable salt thereof, in the treatment of a condition selected from pain, cancer, inflammation, neurodegenerative disease or Trypanosoma cruzi infection. In one embodiment, the condition is pain. In one embodiment, the condition is cancer. In one embodiment, the condition is inflammation. In one embodiment, the condition is a neurodegenerative disease. In one embodiment, the condition is Trypanosoma cruzi infection.

WO 2011/006074 PCT/US2010/041538 53 Examples [00398] The following examples illustrate the invention. In the examples described below, unless otherwise indicated all temperatures are set forth in degrees Celsius. Reagents were purchased from commercial suppliers such as Aldrich Chemical Company, Lancaster, TCI or Maybridge, and were used without further purification unless otherwise indicated. Tetrahydrofuran (THF), dichloromethane (DCM, methylene chloride), toluene, dimethyl formamide (DMF) and dioxane were purchased from Aldrich in Sure/SealTM bottles and used as received. [003991 The reactions set forth below were done generally under a positive pressure of nitrogen or argon or with a drying tube (unless otherwise stated) in anhydrous solvents, and the reaction flasks were typically fitted with rubber septa for the introduction of substrates and reagents via syringe. Glassware was oven dried and/or heat dried. [00400] Column chromatography was done on a Biotage system (Manufacturer: Dyax Corporation) having a silica gel or C-18 reverse phase column, or on a silica SepPak cartridge (Waters). Biological assays [00401] The ability of compounds of the invention to act as TrkA inhibitors may be demonstrated by the assays described in Examples A and B. The ability of compounds of the invention to act as TrkB inhibitors may be demonstrated by the assay described in Example B. [00402] The selectivity of compounds of Formula I for inhibiting TrkA kinase activity over inhibiting one or more JAK kinases was determined using the assays describes in Examples C, D, E and F. Example A TrkA ELISA assay [004031 An enzyme-linked immunosorbant assay (ELISA) was used to assess TrkA kinase activity in the presence of inhibitors. Immulon 4HBX 384-well microtiter plates (Thermo part #8755) were coated with a 0.025 mg/mL solution of poly (Glu, Ala, Tyr; 6:3:1; Sigma P3899). Various concentrations of test compound, 2.5 nM TrkA (Invitrogen Corp., histidine-tagged recombinant human TrkA, cytoplasmic domain), and 500 gM ATP were incubated for 25 minutes at ambient temperature in the coated plates while shaking. The assay buffer consisted of 25 mM MOPS pH 7.5, 0.005% (v/v) Triton X-100 and 5 mM MgCl 2 . The reaction mixture was removed from the plate by washing with PBS containing WO 2011/006074 PCT/US2010/041538 54 0.1% (v/v) Tween 20. The phosphorylated reaction product was detected using 0.2 gg/mL of a phosphotyrosine specific monoclonal antibody (clone PY20) conjugated to horseradish peroxidase in conjunction with the TMB Peroxidase Substrate System (KPL). After the addition of IM phosphoric acid, the chromogenic substrate color intensity was quantitated via absorbance at 450 nm. IC 50 values were calculated using either a 4 or 5-parameter logistic curve fit and are provided in Table 1. Example B TrkA and TrkB Omnia Assay [00404] Trk enzymatic selectivity was assessed using Omnia Kinase Assay reagents from Invitrogen Corp. Enzyme (either TrkA or TrkB from Invitrogen Corp.) and test compound (various concentrations) were incubated for 10 minutes at ambient temperature in a 384-well white polypropylene plate (Nunc catalog# 267462). Omnia Tyr Peptide #4 (for TrkA) or #5 (for TrkB), as well as ATP, were then added to the plate. Final concentrations were as follows: 20 nM enzyme, 500 gM of ATP for TrkA assay or 1 mM ATP for TrkB assay, 10 gM peptide substrate. The assay buffer consisted of 25 mM MOPS pH 7.5, 0.005% (v/v) Triton X-100 and 5 mM MgCl 2 . The production of phosphorylated peptide was monitored continuously for 70 minutes using a Molecular Devices FlexStation 11384 microplate reader (excitation = 360 nm; emission = 485 nm). Initial rates were calculated from the progress curves. IC 50 values were calculated from these rates using either a 4 or 5 parameter logistic curve fit. [004051 In each of TrkA and TrkB Omnia assays, compounds of the invention had an average IC 50 value below 1000 nM. Certain compounds had an average IC 50 value below 100 nM. General JAK kinase Enzyme Inhibition Assay Method [00406] The assays described in Examples C, D, E and F for the determination of JAKI, JAK2, JAK3 and Tyk2 kinase activity, respectively, utilized the Omnia* Kinase fluorescence peptide substrate-based technology (Invitrogen). The specific components of the assay mixture are described in Examples C, D and E. In each of the assays described in Examples C, D and E, Mg 2 + is chelated upon phosphorylation of the Omnia peptide by the kinase to form a bridge between the chelation-enhanced fluorophore Sox and the phosphate, resulting in an increase in fluorescence emission at 485 nM when excited at 360 nM. The reactions were therefore read at excitation 360 nm and emission was measured at 485 nm every 50 seconds for 45 minutes using a PerkinElmer EnVision Multilabel Plate Reader.

WO 2011/006074 PCT/US2010/041538 55 [004071 The final buffer conditions for each of the JAKI, JAK2, JAK3 and Tyk2 assays were as follows: 25 mM HEPES, pH 7.4, 10 mM MgCl 2 , 0.01% Triton X-100 and 1 mM DTT.

IC

50 Determinations: [00408] Compounds were prepared at 50x the final concentration in DMSO by conducting 3-fold serial dilutions from a 500-gM or 1000-gM intermediate dilution to give a 10-point dosing curve having a high dose of 10 or 20 gM. Two-gL aliquots of these were transferred to a fresh plate for a ten-fold intermediate dilution with assay buffer. Five-gL aliquots of the diluted compounds were then transferred to 20-gL of assay mixtures described in Examples C, D, E and F for a final concentration of DMSO of 2%. A standard or reference compound was typically included on each assay plate to validate that plate. For each plate, percent of control (POC) values were calculated for each well according to the following equation: POC= Sample - Xm'" X 100, Xmax - Xmin where X. = Average Uninhibited Controls min= Average Background

IC

50 's were estimated from the POC's using a standard 4-parameter logistic model: YA+ B-A Y+ where A = Minimum Y (Bottom Asymptote) B = Maximum Y (Top Asymptote) C = EC 5 o D = Slope Factor X = Compound Concentration (nM) Y = POC [00409] The IC 5 0 is defined as the concentration of inhibitor at which the POC equals 50 for the fitted curve. Example C Jakl Inhibition Assay [00410] Compounds of Formula I were screened for their ability to inhibit Jak1 using the general enzyme inhibition assay method, in which the assay mixture contained 500 gM WO 2011/006074 PCT/US2010/041538 56 ATP, 8 gM Omnia* Y12 peptide (Catalog # IVGN KPZ3121C; Invitrogen Corporation, Carlsbad, CA) and 5 nM JakI in a total volume of 20 gL. GST-tagged human JakI kinase domain comprising amino acids 866-1154 was purchased from Invitrogen Corporation, Carlsbad, CA (catalog # IVGN PV4774). Results are shown in Table 2. Example D Jak2 Inhibition Assay [00411] Compounds of Formula I were screened for their ability to inhibit Jak2 using the general enzyme inhibition assay method, in which the assay mixture contained 500 gM ATP, 10 gM Omnia* Y7 peptide (Catalog # IVGN KNZ3071C, Invitrogen Corporation, Carlsbad, CA) and 4 nM Jak2 in a total volume of 20 gL. Human Jak2 kinase domain comprising amino acids 808-1132 was purchased from Invitrogen Corporation, Carlsbad, CA (catalog # IVGN PV42 10). Results are shown in Tables 1 and 2. Example E Jak3 Inhibition Assay [00412] Compounds of Formula I were screened for their ability to inhibit Jak3 using the general enzyme inhibition assay method, in which the assay mixture contained 500 gM ATP, 10 gM Omnia* Y7 peptide (Catalog # IVGN KNZ3071C, Invitrogen Corporation, Carlsbad, CA) and 1.5 nM Jak3 in a total volume of 20 gL. GST-tagged human Jak3 kinase domain comprising amino acids 781-1124 was purchased from Invitrogen Corporation, Carlsbad, CA (catalog # IVGN PV3855). Results are shown in Table 2. Example F Tyk2 Inhibition Assay [00413] Compounds of Formula I were screened for their ability to inhibit Tyk2 using the general enzyme inhibition assay method, in which the assay mixture contained 500 gM ATP, 8 gM Omnia* Y12 peptide (Catalog # IVGN KPZ3121C; Invitrogen Corporation, Carlsbad, CA) and 1 nM Tyk2 in a total volume of 20 gL. Human Tyk2 kinase domain, comprising amino acids 886 to 1187 with 10 additional histidine residues (histidine tag) on the carboxy terminus, was expressed and purified from bacculovirus in-house at Array BioPharma Inc. (Boulder, CO). The histidine tag was cleaved after purification using standard conditions. Results are shown in Table 2. [00414] Table 1 provides IC 50 values for compounds of the invention when tested in the assays of Examples A and D. The Jak2 enzyme IC 50 was designated as > 1000 nM when >50% inhibition was not observed at a 1000 nM concentration of test compound.

WO 2011/006074 PCT/US2010/041538 57 Table 1 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 50 (nM) (% inhibition at 1000 nM) 1 0.7 >1000 (39.5) 2 0.7 >1000 (19.1) 3 2.3 >1000 (10.1) 4 0.95 >1000 (18.8) 5 0.95 >1000 (14.2) 6 1.55 >1000 (12.9) 7 0.45 106.8 (90.0) 8 1.1 >1000 (25.7) 9 3.45 >1000 (4.6) 10 1.05 >1000 (47.5) 11 777.1 >1000 (10.1) 12 238.25 >1000 (4.5) 13 0.5 >1000 (41.4) 14 0.55 470 (66.7) 15 0.6 156 (83.1) 16 0.9 >1000 (31.7) 17 2.15 >1000 (5.6) 18 38.3 >1000 (2.8) 19 74.25 >1000 (3.4) 20 0.6 257 (78.9) 21 2.95 >1000 (5.8) WO 2011/006074 PCT/US2010/041538 58 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 50 (nM) (% inhibition at 1000 nM) 22 2.1 >1000 (9.8) 23 1.1 >1000 (10.1) 24 3.6 >1000 (11.5) 25 0.4 >1000 (19.4) 26 1.3 >1000 (9.9) 27 214.5 >1000 (1.3) 28 2.8 >1000 (5.0) 29 1.3 >1000 (13.2) 30 1.95 >1000 (25.7) 31 10.6 >1000 (5.1) 32 5.3 >1000 (8.6) 33 1.5 23.4 (99.9) 34 1.1 42.3 (96.9) 35 1.2 278 (77.4) 36 2.1 >1000 (22.2) 37 1.65 >1000 (25.0) 38 1.3 >1000 (18.2) 39 0.7 >1000 (30.4) 40 0.8 >1000 (41.4) 41 28.9 >1000 (6.0) 42 48.3 >1000 (7.7) 43 139 >1000 (4.2) WO 2011/006074 PCT/US2010/041538 59 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 50 (nM) (% inhibition at 1000 nM) 44 1.5 388 (71.5) 45 1.4 195 (84.0) 46 3.3 >1000 (13.8) 47 1.5 >1000 (37.3) 48 0.6 429 (72.7) 49 4.2 >1000 (17.2) 50 1.23 418 (73.7) 51 1.18 186 (82.5) 52 7.4 >1000 (9.4) 53 10.4 >1000 (8.7) 54 2.25 >1000 (23.0) 55 1 >1000 (24.1) 56 2.4 >1000 (28.0) 57 3.93 >1000 (9.7) 58 9.4 >1000 (4.7) 59 16.95 >1000 (14.5) 60 2.25 >1000 (21.1) 61 1.95 699 (54.4) 62 2.53 >1000 (35.2) 63 4.55 >1000 (33.1) 64 1.6 575 (60.3) 65 0.6 >1000 (34.1) WO 2011/006074 PCT/US2010/041538 60 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 50 (nM) (% inhibition at 1000 nM) 66 0.57 >1000 (24.7) 67 5.1 >1000 (22.3) 68 6.6 >1000 (23.0) 69 60.3 >1000 (6.0) 70 23.95 >1000 (9.0) 71 8.65 >1000 (6.2) 72 44.35 >1000 (11.7) 73 48.55 >1000 (4.9) 74 12.6 >1000 (12.2) 75 6.95 >1000 (15.1) 76 90.05 >1000 (4.8) 77 5.37 >1000 (15.3) 78 34.85 >1000 (5.8) 79 1.3 698 (54.8) 80 1.8 869 (50.7) 81 1.15 666 (54.4) 82 2.55 >1000 (10.7) 83 1.77 >1000 (26.3) 84 21.05 >1000 (2.7) 85 9.38 >1000 (6.0) 86 26.7 >1000 (17.3) 87 16.1 >1000 (37.2) WO 2011/006074 PCT/US2010/041538 61 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 50 (nM) (% inhibition at 1000 nM) 88 6.13 >1000 (15.1) 89 3.6 >1000 (33.1) 90 0.95 472 (67.3) 91 3.2 >1000 (34.9) 92 1013.3 >1000 (0.5) 93 5.1 >1000 (34.7) 94 568.2 >1000 (4.4) 95 5.4 >1000 (19.2) 96 342.5 >1000 (4.8) 97 6.2 >1000 (6.8) 98 9.0 >1000 (23.7) 99 7.0 >1000 (45.8) 100 4.7 >1000 (13.8) 101 11.7 >1000 (26.2) 102 5.2 >1000 (17.3) 103 87.8 >1000 (4.5) 104 83.1 >1000 (6.1) 105 25.4 >1000 (18.7) 106 7.7 >1000 (8) 107 16.4 >1000 (7.1) 108 1191.6 >1000 (2.8) 109 36.7 >1000 (-0.3) WO 2011/006074 PCT/US2010/041538 62 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 50 (nM) (% inhibition at 1000 nM) 110 37.2 >1000 (-0.5) 111 37.8 >1000 (0.1) 112 30.9 >1000 (3.2) 113 3.2 >1000 (44.6) 114 29.9 >1000 (5) 115 15.3 >1000 (12.6) 116 26.2 >1000 (4.7) 117 45.0 >1000 (-1.4) 118 22.5 >1000 (-4.5) 119 131.2 >1000 (1.7) 120 22.8 >1000 (2.3) 121 182.5 >1000 (3.7) 122 33.2 >1000 (7.4) 123 6.4 >1000 (38.8) 124 2.9 759 (53.3) 125 12.8 >1000 (6.1) 126 218.5 >1000 (-1) 127 469.8 >1000 (0.2) 128 2595.0 >1000 (1.2) 129 8.0 >1000 (25.2) 130 1.7 >1000 (27.9) 131 5.0 >1000 (14.6) WO 2011/006074 PCT/US2010/041538 63 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 50 (nM) (% inhibition at 1000 nM) 132 44.4 >1000 (3.7) 133 16.0 >1000 (14.4) 134 7.4 >1000 (3.3) 135 142.2 >1000 (-1) 136 26.3 >1000 (4.8) 137 793.7 >1000 (3.6) 138 34.8 >1000 (10) 139 32.7 >1000 (-0.4) 140 0.9 76.7 (90.1) 141 9.4 >1000 (9.4) 142 12.7 >1000 (-0.9) 143 8.0 >1000 (7) 144 not tested not tested 145 not tested not tested 146 not tested not tested 147 30.8 >1000 (0.9) 148 1.1 >1000 (44.3) 149 6.1 >1000 (10.2) 150 5.7 >1000 (8.9) 151 1.4 >1000 (35.6) 152 18.1 >1000 (7.6) 153 2.1 >1000 (33.8) WO 2011/006074 PCT/US2010/041538 64 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 50 (nM) (% inhibition at 1000 nM) 154 1.3 524 (64.4) 155 0.2 >1000 (33.3) 156 2.1 >1000 (16.1) 157 2.8 >1000 (9.6) 158 9.9 >1000 (14.4) 159 4.3 >1000 (28.9) 160 25.6 >1000 (8.5) 161 3.0 >1000 (27.4) 162 2.2 444 (66.2) 163 not tested not tested 164 not tested not tested 165 1.1 >1000 (26.8) 166 2.4 >1000 (13.8) 167 2.2 >1000 (9.8) 168 1.2 >1000 (23.2) 169 0.9 759 (51.9) 170 11.6 >1000 (0.7) 171 4.7 >1000 (7.8) 172 2.5 >1000 (11) 173 1.5 >1000 (5.4) 174 16.3 >1000 (6.4) 175 12.2 >1000 (3.8) WO 2011/006074 PCT/US2010/041538 65 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 50 (nM) (% inhibition at 1000 nM) 176 27.2 >1000 (6.3) 177 1.8 >1000 (26.2) 178 2.5 >1000 (16.9) 179 8.5 >1000 (-0.2) 180 12.3 >1000 (-0.8) 181 17.1 >1000 (5.2) 182 11.3 >1000 (21.2) 183 6.9 >1000 (-0.8) 184 7.4 >1000 (11.8) 185 8.6 >1000 (11.7) 186 57.1 >1000 (10.5) 187 61.6 >1000 (9.1) 188 83.0 >1000 (9.3) 189 76.4 >1000 (4.6) 190 2.4 >1000 (28.4) 191 69.5 >1000 (4.8) 192 437.8 >1000 (0.3) 193 15.6 >1000 (8.4) 194 4.7 >1000 (31.2) 195 7.7 >1000 (16.2) 196 6.8 >1000 (10.6) 197 4.8 >1000 (3.6) WO 2011/006074 PCT/US2010/041538 66 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 50 (nM) (% inhibition at 1000 nM) 198 242.8 >1000 (1.6) 199 3.6 >1000 (38.8) 200 12.7 >1000 (12.5) 201 71.8 >1000 (3.8) 202 19.3 >1000 (11.5) 203 not tested not tested 204 16.6 >1000 (37) 205 14.3 >1000 (7.2) 206 3.9 >1000 (12.6) 207 33.3 >1000 (0.9) 208 1.7 >1000 (21.2) 209 17.1 >1000 (5.4) 210 3.3 >1000 (32.3) 211 4.2 >1000 (19.5) 212 38.0 >1000 (14.2) 213 6.8 >1000 (21.9) 214 8.6 >1000 (29.7) 215 15.3 >1000 (28.3) 216 3.1 670 (54) 217 5.8 551 (61.7) 218 33.9 >1000 (24.6) 219 187.7 >1000 (25.5) WO 2011/006074 PCT/US2010/041538 67 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 50 (nM) (% inhibition at 1000 nM) 220 109.9 >1000 (15.2) 221 49.3 >1000 (-1.2) 222 not tested not tested 223 not tested not tested 224 not tested not tested 225 52.4 >1000 (2.7) 226 10.5 >1000 (100) 227 12.3 445.8 (62.8) 228 13.0 >1000 (31.9) 229 15.9 >1000 (34.9) 230 3.0 665.6 (53.3) 231 8.7 >1000 (26.6) 232 4.5 >1000 (31.9) 233 75.9 >1000 (6.7) 234 10.7 >1000 (5.3) 235 2.8 311 (73.1) 236 2.2 419 (69.1) 237 2.1 616 (51.9) 238 1.9 499 (58.9) 239 10.1 >1000 (15.5) 240 11.3 not tested 241 21.8 not tested 242 8.9 not tested 243 9 not tested WO 2011/006074 PCT/US2010/041538 68 Example # TrkA Enzyme Jak2 Enzyme

IC

50 (nM) IC 5 0 (nM) (% inhibition at 1000 nM) 244 38.2 not tested [00415] Representative compounds of the invention were tested in the four Jak Kinase enzyme assays described in Examples C, D, E and F. The IC 50 values are shown in Table 2. These compounds were found to be even more selective for inhibiting TrkA kinase activity over inhibiting kinase activity of Jakl, Jak3 and Tyk2 than over inhibiting Jak2. Table 2 Ex # TrkA Jakl Jak2 Jak3 Tyk2

IC

5 0

IC

50 (nM) IC 50 (nM) IC 50 (nM) IC 50 (nM) (nM) (% inhibition (% inhibition (% inhibition (% inhibition at 1000 nM) at 1000 nM) at 1000 nM) at 1000 nM) 30 1.9 >1000 >1000 >1000 >1000 (13.4) (30.4) (2.9) (11.3) 52 7.4 >1000 >1000 >1000 >1000 (8.6) (13.0) (0.8) (13.8) 140 0.9 546 76.7 >1000 >1000 (64.2) (98.5) (20.2) (34.8) 93 5.1 >1000 >1000 >1000 >1000 (19.7) (42.2) (10.6) (17.2) 106 7.6 >1000 >1000 >1000 >1000 (8.2) (21.0) (9.7) (14.8) 114 17.1 >1000 >1000 >1000 >1000 (10.9) (15.6) (8.5) (11.4) 181 29.8 >1000 >1000 >1000 >1000 (12.8) (18.1) (8.9) (10.7) 91 3.2 >1000 >1000 >1000 >1000 (20.3) (42.1) (8.3) (14.8) 123 6.3 >1000 >1000 >1000 >1000 (22.0) (49.1) (8.9) (14.4) 124 2.9 >1000 759 >1000 >1000 (36.4) (72.3) (7.2) (16.2) 190 2.4 >1000 >1000 >1000 >1000 (14.3) (33.9) (7.2) (13.4) 98 9.0 >1000 >1000 >1000 >1000 (8.8) (27.8) (5.5) (9.2) 194 4.6 >1000 >1000 >1000 >1000 (7.4) (37.6) (2.6) (8.1) Preparation A WO 2011/006074 PCT/US2010/041538 69 NH F F (R)-2-(2,5-difluorophenyl)yrrolidine [00416] Step A: Preparation of (R)-tert-butvl 2-(2,5-difluorophenvl)pyrrolidine-1 carboxylate. A solution of tert-butyl pyrrolidine-1-carboxylate (20 g, 116.8 mmol) and (-) sparteine (32.9, 140 mmol) in MTBE (360 mL) was cooled to -78 'C and sec-BuLi (100 mL, 140 mmol, 1.4 M in cyclohexane) was introduced drop-wise via cannula, keeping the internal temperature under -70 'C. The resulting solution was stirred for 3 hours at -78 'C, followed by addition of a solution of ZnCl 2 (93.4 mL, 93.4 mmol, IM in Et 2 0) drop-wise with rapid stirring, keeping the internal temperature below -65 'C. The resulting light suspension was stirred at -78 'C for 30 minutes and then warmed to ambient temperature. The resulting mixture was sequentially charged with 2-bromo-1,4-difluorobenzene (14.5 mL, 128 mmol), Pd(OAc) 2 (1.31 g, 5.8 mmol) and t-Bu 3

P-HBF

4 (2.03 g, 7.0 mmol) in one portion. After stirring overnight at ambient temperature, concentrated NH 4 0H (10.5 mL) was added and the reaction was stirred for 1 hour. The resulting slurry was filtered through Celite and the filter cake washed with Et 2 0 (1 L). The filtrate was washed with a IM aqueous HCl solution (0.5 L) and brine. The organic layer was filtered and concentrated, and the crude product was purified by silica column chromatography, eluting with 5-10% EtOAc/hexanes to give product (R)-tert-butyl 2-(2,5-difluorophenyl)pyrrolidine-1-carboxylate as yellow oil (23.9 g, 72% yield). [004171 Step B: Preparation of (R)-2-(2,5-difluorophenvll)pyrrolidine. To (R)-tert butyl 2-(2,5-difluorophenyl)pyrrolidine-1-carboxylate (23.9 g, 84.4 mmol) was added 4N HCl in dioxane (56.2 mL). After stirring at ambient temperature for 2 hours, ether (200 mL) was added and the mixture was stirred for 10 minutes. The resulting slurry was filtered, yielding the title compound hydrochloride salt as a white solid (17.2 g). To obtain the free base, the HCl salt product was dispersed in a mixture of EtOAc (200 mL) and NaOH solution (100 mL, 2 N aq.) The layers were separated and the aqueous layer was extracted with EtOAc. The combined organic extracts were filtered and concentrated to give the desired product as a liquid (13.2 g, 85% yield). [00418] The enantiomeric excess (% ee) of (R)-2-(2,5-difluorophenyl)pyrrolidine was determined as follows: To an ethanol solution of (R)-2-(2,5-difluorophenyl)pyrrolidine was added excess N-(2,4-dinitro-5-fluorophenyl)-L-alanine amide (FDAA, Marfey's reagent).

WO 2011/006074 PCT/US2010/041538 70 The mixture was heated to reflux for approximately two minutes. After cooling to ambient temperature, the reaction mixture was diluted with acetonitrile and analyzed by HPLC (YMC ODS-AQ 4.6 x 50 mm 3 gm 120A column; mobile phase: 5-95% solvent B in A; solvent A:

H

2 0/1% iPrOH/10 mM ammonium acetate, and solvent B: ACN/1% iPrOH /10 mM ammonium acetate; flow rate: 2 mL/min). The enantiomeric excess (ee%) was determined from the peak areas of the two diastereomeric derivatives formed. A 1:1 racemic standard was prepared according the same procedure described herein, replacing (R)-2-(2,5 difluorophenyl)pyrrolidine with (rac)-2-(2,5-difluorophenyl)pyrrolidine. The ee% of the title compound obtained as described above was determined to be > 93%. Preparation B N CI N C02Et Ethyl 5-chloropyrazolo[1,5-alpyrimidine-3-carboxylate [00419] Step A: Preparation of ethyl 5-hydroxypyrazolo[1,5-alpyrimidine-3 carboxylate. Ethyl 3-amino-1H-pyrazole-4-carboxylate (25.0 g, 161 mmol) and (E)-ethyl 3 ethoxyacrylate (35.8 ml, 242 mmol) were mixed in DMF (537 mL). Cesium carbonate (78.7 g, 242 mmol) was added and the mixture heated to 110 'C for 15 hours. The reaction mixture was cooled to ambient temperature and acidified with HOAc to pH 4. The resultant precipitate was filtered and washed with water and EtOAc, yielding the title compound as a fluffy white solid. Additional material was obtained by an aqueous workup. The filtrate was concentrated to remove the DMF, was diluted in EtOAc (500 mL) and washed with H 2 0. The resultant precipitate in the EtOAc layer was filtered and washed with water and EtOAc to obtain additional product. The solids were pooled and dried in vacuum to afford ethyl 5 hydroxypyrazolo[1,5-a]pyrimidine-3-carboxylate (33.3 g, 100 % yield) as a fluffy white solid. MS (apci) m/z = 206.2 (M-H). [00420] Step B: Preparation of ethyl 5-chloropyrazolo[1,5-alpyrimidine-3-carboxylate. Ethyl 5-hydroxypyrazolo[1,5-a]pyrimidine-3-carboxylate (22.7 g, 110 mmol) was suspended in phosphoryl trichloride (100 mL) and heated to reflux. After heating for 2 hours, the reaction mixture was cooled and concentrated to remove the excess POCl 3 . The residue was diluted in DCM (100 mL) and slowly added to a flask containing ice water. The mixture was separated and the aqueous layer extracted with DCM. The combined organics were dried WO 2011/006074 PCT/US2010/041538 71 with MgSO 4 , filtered and concentrated to afford ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3 carboxylate (24.2 g, 97.6 % yield) as a pale yellow solid. MS (apci) m/z = 225.9 (M+H). Preparation C N - N N N \ CO2H \F F (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]lpyrimidine-3-carboxylic acid [00421] Step A: Preparation of (R)-ethyl 5-(2-(2,5-difluorophenyl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxylate. A mixture of ethyl 5-chloropyrazolo[1,5 a]pyrimidine-3-carboxylate (Preparation B, 2.00 g, 8.86 mmol), (R)-2-(2,5 difluorophenyl)pyrrolidine (Preparation A, 1.62 g, 8.86 mmol), diisopropylethylamine (3.09 mL, 17.7 mmol) and butan-1-ol (2.95 ml, 8.86 mmol) was heated at 100 'C for 15 hours. The reaction mixture was cooled to ambient temperature and was diluted with EtOAc (30 mL) and water (10 mL). Undissolved solid was filtered and washed with Et 2 0 to afford the title compound as a light orange solid (2.13 g). The organic layer was separated from the filtrate, washed with brine (10 mL) and dried over MgSO 4 . The solution was filtered and concentrated to provide additional solid that was purified by silica chromatography using gradient elution with 50-100% EtOAc/hexanes. This afforded the title compound (0.50 g) as a light yellow solid. The combined yield was 2.63 g, 79.7 %. MS (apci) m/z = 373.1 (M+H). [00422] Step B: Preparation of (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. (R)-ethyl 5-(2-(2,5 difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (2.13 g, 5.72 mmol) was suspended in EtOH (28.6 mL) and heated at 90 'C for 20 min (homogeneous). IM aq. LiOH (11.4 mL, 11.4 mmol) was added and the reaction mixture was heated for 15 hours at 90 'C. After cooling, the reaction mixture was concentrated, diluted with water and washed with EtOAc to remove any unreacted starting material. The aqueous layer was then acidified to pH 1 using 2N HCl. After extracting with DCM and EtOAc, the combined organic fractions were dried with MgSO 4 , filtered and concentrated to afford (R)-5-(2-(2,5 difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (1.82 g, 92.4 %) as a light yellow solid. MS (apci) m/z = 345.0 (M+H).

WO 2011/006074 PCT/US2010/041538 72 Preparation D NH (R)-2-(3-fluorophenyl)yrrolidine [00423] Prepared by the method of Preparation A, substituting 2-bromo-1,4 difluorobenzene with 1-bromo-3-fluorobenzene in Step A. MS (apci) m/z = 166.0 (M+H). The ee% of the title compound was determined to be 94 %. Preparation E N - N N N CO2H F (R)-5-(2-(3-fluorophenvl)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid [00424] Step A: Preparation of (R)-ethyl 5-(2-(3-fluorophenvl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxylate. Prepared according to the method of Preparation C, substituting (R)-2-(2,5-difluorophenyl)pyrrolidine in Step A with (R)-2-(3 fluorophenyl)pyrrolidine. MS (apci) m/z = 355.0 (M+H). [004251 Step B: Preparation of (R)-5-(2-(3-fluorophenvl)pyrrolidin-1-Vl)pyrazolo[1,5 alpyrimidine-3-carboxylic acid. (R)-ethyl 5-(2-(3-fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylate (0.76 g, 2.14 mmol) was suspended in EtOH (10.7 mL) and the mixture was heated at 90 'C for 20 minutes (homogeneous). IM aqueous LiOH (4.29 ml, 4.29 mmol) was added and the reaction mixture was heated for 15 hours at 90 'C. After cooling, the reaction mixture was concentrated, diluted with water and washed with EtOAc to remove any unreacted starting material. The aqueous layer was then acidified to pH 4 using 2N HCl. After extracting with DCM and EtOAc, the combined organic layers were dried with MgSO 4 , filtered and concentrated to afford (R)-5-(2-(3-fluorophenyl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (0.60 g, 85.7 % yield) as a glassy yellow solid. MS (apci) m/z = 327.0 (M+H).

WO 2011/006074 PCT/US2010/041538 73 Preparation F NH

H

3 CO (R)-2-(5-fluoro-2-methoxvphenvll)pyrrolidine [00426] Prepared by the method of Preparation A, substituting 2-bromo-1,4 difluorobenzene with 2-bromo-4-fluoro-1-methoxybenzene in Step A. MS (apci) m/z = 196.1 (M+H). The ee% of the title compound was determined to be >99 %. Preparation G N N N \ C0 2 H F

H

3 CO (R)-5-(2-(5-fluoro-2-methoxvphenvll)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid [004271 In a sealed tube, ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate (Preparation B, 500 mg, 2.22 mmol), (R)-2-(5-fluoro-2-methoxyphenyl)pyrrolidine hydrochloride salt (513 mg, 2.22 mmol), and diisopropylethylamine (0.774 mL, 4.43 mmol) were combined in isopropanol (2 mL) and heated at 160 'C for 3 days. 2N NaOH (6 mL) and MeOH (5 mL) were added and the reaction mixture stirred at ambient temperature for 24 hours, followed by heating to 40 'C for 3 hours. The reaction was partially concentrated, treated with saturated aqueous NH 4 Cl (10 mL) and the mixture extracted with EtOAc. The combined organic extracts were filtered, concentrated and the residue purified by reverse phase chromatography eluting with 0

-

6 0 % acetonitrile/water to yield the title compound as a pink solid (254 mg, 32.2% yield). MS (apci) m/z = 357.0 (M+H). Preparation H NH F N (R)-3-fluoro-5-(pyrrolidin-2-yl)pyridine WO 2011/006074 PCT/US2010/041538 74 [00428] Prepared by the method of Preparation A, substituting 2-bromo-1,4 difluorobenzene with 3-bromo-5-fluoropyridine in Step A. MS (apci) m/z = 167.1 (M+H). The ee% of the title compound was determined to be 92%. Preparation I N - N N N CO2H F N (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid [00429] Step A: Preparation of ethyl 5-(2-(5-fluoropyridin-3-Vl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxylate. Ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3 carboxylate (Preparation B; 0.50 g, 2.22 mmol), (R)-3-fluoro-5-(pyrrolidin-2-yl)pyridine dihydrochloride (0.53 g, 2.22 mmol) and diisopropylethylamine (1.46 mL, 8.86 mmol) were combined in isopropanol (2 mL) and heated at 95 'C for 70 hours. The crude product was purified by reverse phase chromatography, eluting with 0-50% acetonitrile/water to yield the title compound (540 mg, 68.6% yield). MS (apci) m/z = 356.0 (M+H). [00430] Step B: Preparation of 5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. Ethyl 5-(2-(5-fluoropyridin-3-yl)pyrrolidin 1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (0.540 g, 1.52 mmol) was dissolved in MeOH (20 mL) and treated with IN NaOH (13 mL). After stirring for 5 days, citric acid (solid) was added to acidify the mixture to pH 4-5. Saturated aqueous NaCl (10 mL) was added and the reaction mixture extracted with DCM and EtOAc. The combined organic layers were combined to afford 5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxylic acid (0.49 g, 99% yield). MS (apci) m/z = 328.0 (M+H). Preparation J NH F

H

3 CO N (R)-5-fluoro-2-methoxy-3-(pyrrolidin-2-yl)pyridine [00431] Step A: Preparation of 3-bromo-5-fluoro-2-methoxvpyridine. 3-Bromo-5 fluoropyridin-2(1H)-one (10.0 g, 52.1 mmol) and Ag 2

CO

3 (10.0 g, 36.5 mmol) were combined in toluene (100 mL) and iodomethane (3.89 mL, 62.5 mmol) was added drop-wise.

WO 2011/006074 PCT/US2010/041538 75 The reaction was stirred at ambient temperature overnight, filtered through Celite and the solids were washed with toluene. The filtrate was concentrated and the residue was purified on a silica gel column (5-25% EtOAc/hexanes) to afford 3-bromo-5-fluoro-2 methoxypyridine (4.70 g, 43.8%) as a clear oil. [00432] Step B: Preparation of (R)-5-fluoro-2-methoxy-3-(pyrrolidin-2-yl)yridine. Prepared by the method of Preparation A, substituting 2-bromo-1,4-difluorobenzene with 3 bromo-5-fluoro-2-methoxypyridine in Step A. MS (apci) m/z = 197.1 (M+H). The ee% of the title compound was determined to be 98 %. Preparation K N N N C0 2 H F

H

3 CO N (R)-5-(2-(5-fluoro-2-methoxvpyridin-3-vl)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3 carboxylic acid [00433] Step A: Preparation of (R)-ethyl 5-(2-(5-fluoro-2-methoxvpyridin-3 yl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylate. Ethyl 5-chloropyrazolo[1,5 a]pyrimidine-3-carboxylate (Preparation B, 0.75 g, 3.32 mmol), (R)-5-fluoro-2-methoxy-3 (pyrrolidin-2-yl)pyridine dihydrochloride (0.984 g, 3.66 mmol), diisopropylethylamine (2.32 mL, 13.3 mmol) and n-butanol (1.11 mL) were heated at 90 'C for 48 hours. The reaction mixture was diluted with EtOAc and the mixture was washed with water, brine and saturated NaHCO 3 . The organic layer was dried with MgSO 4 , filtered and concentrated afford a dark orange oil. The oil was purified by silica chromatography, eluting with a 50-80% EtOAc/Hexane gradient, to afford (R)-ethyl 5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin 1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (0.72 g, 56.2 %) as a yellow foam. MS (apci) m/z = 386.0 (M+H). [00434] Step B: (R)-5-(2-(5-fluoro-2-methoxvpyridin-3-vl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. To a suspension of (R)-ethyl 5-(2-(5 fluoro-2-methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (0.72 g, 1.868 mmol) in MeOH (9.34 mL) was added IN LiOH (3.74 ml, 3.74 mmol) and the reaction mixture heated to 70 'C for 15 hours. After cooling, the reaction mixture was concentrated and the resulting residue diluted in water. After acidifying with citric acid (solid), the aqueous layer was extracted with DCM. The combined organics were dried with WO 2011/006074 PCT/US2010/041538 76 MgSO 4 , filtered and concentrated to afforded (R)-5-(2-(5-fluoro-2-methoxypyridin-3 yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (0.67 g, 100%) as a yellow solid. MS (apci) m/z = 357.9 (M+H). Preparation L NH F _CF

F

3 C (R)-2-(5-fluoro-2-(trifluoromethyl)phenyl)yrrolidine [004351 Prepared by the method of Preparation A, substituting 2-bromo-1,4 difluorobenzene with 2-bromo-4-fluoro-1-(trifluoromethyl)benzene in Step A. MS (apci) m/z = 234.1 (M+H). The ee% of the title compound was determined to be 90 %. Preparation M N - N N N CO2H F

F

3 C (R)-5 -(2-(5 -fluoro-2-(trifluoromethyl)phenyl)pyrrolidin- 1 -yl)pyrazolo 1,5 -alpyrimidine-3 carboxylic acid [00436] Step A: Preparation of (R)-ethyl 5-(2-(5-fluoro-2-(trifluoromethyl)phenyl) pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3-carboxylate. Ethyl 5-chloropyrazolo[1,5 a]pyrimidine-3-carboxylate (Preparation B, 0.51 g, 2.26 mmol), (R)-2-(5-fluoro-2 (trifluoromethyl)phenyl)pyrrolidine hydrochloride (0.610 g, 2.26 mmol) and diisopropylethylamine (1.12 mL, 6.78 mmol) were suspended in isopropanol (2.5 mL) and heated to 120 'C for 24 hours. The reaction mixture was purified by reverse phase chromatography eluting with 0

-

7 5 % acetonitrile/water to yield the title compound (0.92 g, 96.4% yield). MS (apci) m/z = 423.0.0 (M+H). [004371 Step B: Preparation of 5-(2-(5-fluoro-2-(trifluoromethyl)phenvl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. (R)-ethyl 5-(2-(5-fluoro-2-(trifluoromethyl) phenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (0.92 g, 2.2 mmol) was combined with IN NaOH (25 mL) and MeOH (40 mL). The reaction mixture was stirred at ambient temperature for 20 hours, followed by heating to 40 0 C until complete. Citric acid (solid) was added until the mixture was pH 4-5. Brine (10 mL) was added and this was WO 2011/006074 PCT/US2010/041538 77 extracted with DCM and EtOAc. The combined organic layers were concentrated and the crude product was purified by reverse phase silica gel column chromatography eluting with 0-60% acetonitrile/water to yield 5-(2-(5-fluoro-2-(trifluoromethyl)phenyl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (0.45 g, 52%). MS (apci) m/z = 395.0 (M+H). Preparation N NH NF N (R)-5-fluoro-2-methyl-3-(pyrrolidin-2-yl)pyridine [00438] Step A: Preparation of 3-bromo-5-fluoro-2-methylpyridine: 2,3-Dibromo-5 fluoropyridine (5.0 g, 19.6 mmol), Pd(PPh 3

)

4 (1.13 g, 0.98 mmol) and methyl boronic acid (3.52 g, 58.9 mmol) were combined in dioxane (50 mL) then treated with K 2 C0 3 (8.13, 58.9 mmol) and water (10 mL). The mixture was purged with N 2 then heated to 110 0 C in a sealed vessel for 16 hours. The cooled mixture was partitioned between water (100 mL) and EtOAc (50 mL) and the layers separated. The aqueous layer was extracted with EtOAc (2 x 50 mL) and the combined organic phases were washed with brine (50 mL), dried over Na 2

SO

4 , filtered and concentrated. The residue was purified by silica gel column chromatography eluting with 1-3% EtOAc/hexanes to afford the product as a white solid (1.20 g, 32% yield). MS (apci) m/z = 190.2 (M+). [00439] Step B: Preparation of (R)-5-fluoro-2-methyl-3-(pyrrolidin-2-vl)pyridine: Prepared by the method of Preparation A, substituting 2-bromo-1,4-difluorobenzene with 3 bromo-5-fluoro-2-methylpyridine in Step A. MS (apci) m/z = 181.1 (M+H). Preparation 0 N N N Ny C0 2 H F N (R)-5-(2-(5-fluoro-2-methylpyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3 carboxylic acid [00440] Step A: Preparation of (R)-ethyl 5-(2-(5-fluoro-2-methylpyridin-3 yl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylate: To a solution of ethyl 5- WO 2011/006074 PCT/US2010/041538 78 hydroxypyrazolo[1,5-a]pyrimidine-3-carboxylate (Preparation B, Step A, 372 mg, 1.8 mmol) in DMF (10 mL) was added (benzotriazol-1-yloxy)tris(dimethylamino)phosphonium hexafluorophosphate (874 mg, 1.98 mmol). The mixture was stirred at ambient temperature for 10 minutes then treated with DIEA (1.57 mL, 8.99 mmol) and (R)-5-fluoro-2-methyl-3 (pyrrolidin-2-yl)pyridine dihydrochloride (455 mg, 1.80 mmol). After stirring at ambient temperature for 4 hours the mixture was partitioned between 10% citric acid (50 mL) and EtOAc (50 mL). The layers were separated and the aqueous layer was extracted with EtOAc (2 x 30 mL). The combined organic phases were washed successively with water (30 mL), saturated NaHCO 3 (30 mL), water (30 mL) and brine (2 x 30 mL), then dried over Na 2

SO

4 , filtered and concentrated. The residue was purified by silica gel column chromatography eluting with 1% MeOH/DCM to afford the product as white foam (480 mg, 72% yield). MS (apci) m/z = 370.0 (M+H). [00441] Step B: Preparation of (R)-5-(2-(5-fluoro-2-methylpyridin-3-yl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid: To a solution of (R)-ethyl 5-(2-(5-fluoro-2 methylpyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (480 mg, 1.3 mmol) in a 1:1:1 mixture of THF:MeOH:water (30 mL) was added lithium hydroxide monohydrate (164 mg, 3.9 mmol). The mixture was stirred at ambient temperature for 16 hours then concentrated to 1/3 volume, acidified to pH 3 with IN HCl and extracted with EtOAc (3 x 30 mL). The combined organic phases were washed with brine (20 mL), dried over Na 2

SO

4 , filtered and concentrated to afford the title product as a white solid (381 mg, 86% yield). MS (apci) m/z = 342.0 (M+H). Preparation P NH F N (R)-2-ethyl-5-fluoro-3-(pyrrolidin-2-yl)pyridine [00442] Prepared by the method of Preparation N, substituting methyl boronic acid with ethyl boronic acid in Step A. MS (apci) m/z = 195.1 (M+H).

WO 2011/006074 PCT/US2010/041538 79 Preparation Q N N Ny C0 2 H F N (R)-5-(2-(2-ethyl-5-fluoropyridin-3-yl)pyrrolidin-1-Yl)yrazolo[1,5-alpyrimidine-3 carboxylic acid [00443] Prepared by the method of Preparation 0, substituting (R)-5-fluoro-2-methyl 3-(pyrrolidin-2-yl)pyridine dihydrochloride with (R)-2-ethyl-5-fluoro-3-(pyrrolidin-2 yl)pyridine dihydrochloride in Step A. MS (apci) m/z = 356.0 (M+H). Preparation R F N N N OH 0 (R)-5 -(2-(5 -fluoro- 1 -methyl-2-oxo- 1,2-dihydropyridin-3 -yl)pyrrolidin- 1 -yl)pyrazolo 1,5 alpyrimidine-3-carboxylic acid [00444] Step A: Preparation of (R)-ethyl 5-(2-(5-fluoro-2-oxo-1,2-dihydropyridin-3 yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate. To a mixture of (R)-ethyl 5-(2 (5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (1.0 g, 2.60 mmol, Preparation K, Step A) and AcOH (7.44 mL, 130 mmol) was added HBr (4.76 mL, 33 wt% in acetic acid, 26 mmol) at ambient temperature. The reaction mixture was heated at 90 'C for 2 hours. After cooling, the reaction mixture was diluted with EtOAc, washed with water, saturated NaHCO3, and brine, dried with MgSO4, filtered and concentrated. The crude material was purified by silica column chromatography, eluting with 2-3% MeOH/DCM to yield the title product (0.73 g, 76%). MS (apci) m/z = 372.0 (M+H). [004451 Step B: Preparation of (R)-ethyl 5-(2-(5-fluoro-1-methyl-2-oxo-1,2 dihydropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate. To a suspension of (R)-ethyl 5-(2-(5-fluoro-2-oxo-1,2-dihydropyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (0.73 g, 1.97 mmol) in DMF (10 mL) at 0 'C was added LiH (20 mg, 2.36 mmol). After stirring for 30 minutes, a solution of Mel (0.56 g, WO 2011/006074 PCT/US2010/041538 80 3.93 mmol) in DMF (2 mL) was added and the reaction was stirred at ambient temperature for 17 hours. The reaction mixture was cooled to 0 'C and quenched with ice-water (30 mL). The mixture was extracted with EtOAc (3x), washed with water and brine, dried with MgSO4, filtered and concentrated. The crude material was purified by silica column chromatography, eluting with 2.5% MeOH/DCM to yield the title product (0.64 g, 85%). MS (apci) m/z = 386.0 (M+H). [004461 Step C: Preparation of (R)-5-(2-(5-fluoro-1-methyl-2-oxo-1,2-dihydropyridin 3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid. Prepared by the method described in Preparation K, Step B using (R)-ethyl 5-(2-(5-fluoro-1-methyl-2-oxo-1,2 dihydropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate to yield the title compound (0.571 g, 96% yield). MS (apci) m/z = 358.0 (M+H). Example 1 F F - -N\ N N NH 0 (R)-N-tert-butyl-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3 carboxamide [004471 To a solution of (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid (Preparation C, 20.0 mg, 0.058 mmol) and HATU (24.3 mg, 0.064 mmol) in dry DMF (0.4 mL) was added tert-butyl amine (12.7 mg, 0.174 mmol) followed by diisopropylethylamine (22.5 mg, 0.174 mmol). The mixture was stirred under an atmosphere of N 2 for 18 hours and was added to H 2 0 (3 mL) and mixed. The mixture was extracted with EtOAc and combined extracts were washed with IM HCl, H 2 0, saturated NaHCO 3 and dried over MgSO 4 . The solution was eluted through a SPE SiOH column eluting first with 50% EtOAc-hexanes then with EtOAc. The EtOAc pool was concentrated and the residual colorless glass was treated with hexanes give a white suspension. The hexanes were removed, and the solid was washed with hexanes and dried in vacuum to afford the title compound as a white solid (20 mg, 90%). MS (apci) m/z = 400.1 (M+H).

WO 2011/006074 PCT/US2010/041538 81 Example 2 F F N'N 1N NH NH (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-vl)-N-(pyridin-2-vl)pyrazolo[1,5-alpyrimidine-3 carboxamide [00448] The title compound was prepared according to the procedure outlined for Example 1, using 2-aminopyridine (2 equivalents) heating at 90 'C for 7 hours. The crude material was purified out by SiO 2 column chromatography (50% EtOAc-hexanes) to give the title compound as a white solid (45% yield). MS (apci) m/z = 421.1 (M+H). Example 3 F F N-N cJN N H NH (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)-N-(3-methylpyridin-2-yl)lpyrazolo[1,5 alpyrimidine-3-carboxamide [00449] To a suspension of (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid (Preparation C, 25.0 mg, 0.072 mmol) in CC 4 (1.0 mL) was added thionyl chloride (0.10 mL) and the mixture heated at reflux for 4 hours. The mixture was cooled to ambient temperature and was concentrated to a brittle foam. The foam was dissolved in pyridine (2 mL), 2-amino-3-methyl-pyridine (9.3 mg, 0.086 mmol) was added and the mixture was heated at 90 0 C for 20 hours. The reaction was cooled to ambient temperature and the pyridine evaporated. The residue was partitioned into IM NaOH and EtOAc, mixed and the EtOAc layer removed. The aqueous layer was extracted with EtOAc and combined EtOAc fractions were washed with H 2 0, saturated NaCl and dried over MgSO 4 . The solution was filtered and concentrated, and the resulting solid was washed with dry Et 2 0 to afford the title compound as a white solid (7 mg, 29%). MS (apci) m/z = 435.1

(M+H).

WO 2011/006074 PCT/US2010/041538 82 Example 4 F F - NN' N N N NH 0 (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-yl)-N-(2-morpholinoethyl)pyrazolo[1,5 alpyrimidine-3-carboxamide [004501 The title compound was prepared according to the procedure outlined for Example 1 using 2-morpholinoethanamine (1.5 equiv). The combined EtOAc extracts were washed with IM Na 2

CO

3 , H 2 0, saturated NaCl and dried over MgSO 4 . The solution was filtered through a SPE SiOH column eluting first with EtOAc and then with 10% MeOH/EtOAc. The MeOH/EtOAc pool was concentrated and the residual colorless glass was triturated with hexanes to give fine white precipitate. The solvent was decanted and the solid was washed with hexanes and dried in vacuum. This afforded the title compound as a white solid (79%). MS (apci) m/z = 457.1 (M+H). Example 5 F N 8NH 0 0-\ol (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-yl)-N-((5-methylfuran-2-vl)methyl)pyrazolo[1,5 alpyrimidine-3-carboxamide [004511 The title compound was prepared according to the procedure outlined for Example 1 using (5-methylfuran-2-yl)methanamine (1.5 equiv.) The dried EtOAc solution was filtered through a packed Celite plug and concentrated. The residual colorless glass was treated with Et 2 0 until dissolved then diluted with hexanes to give a white suspension. Solvents were decanted, the solid washed with hexanes and dried in vacuum. This provided the title compound as a white solid (43% yield). MS (apci) m/z = 438.1 (M+H).

WO 2011/006074 PCT/US2010/041538 83 Example 6 F F N NH N NH, N (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-yl)-N-(1-methyl-iH-pyrazol-3-Vl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00452] The title compound was prepared according to the procedure outlined for Example 3 using 1-methyl-1H-pyrazol-3-amine (1.5 equiv.) at ambient temperature for 64 hours. The crude EtOAc solution was eluted through a SPE SiOH column (EtOAc elution) and concentrated. The residual white solid was washed with 10% Et 2 0-hexanes and dried in vacuum to afford the title compound (47% yield). MS (apci) m/z = 424.1 (M+H). Example 7 F F cN N NH 06 OH 5-((R)-2-(2,5-difluorophenvll)pyrrolidin-1-vl)-N-((trans)-4-hydroxvcvclohexvl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00453] The title compound was prepared according to the procedure outlined for Example 1 using trans-4-aminocyclohexanol (1.5 equiv). The combined EtOAc extracts were washed with IM Na 2

CO

3 , H 2 0, saturated NaCl and dried over MgSO 4 . The solution was filtered through a Celite plug, concentrated and the residual colorless glass was treated with hexanes to give a white suspension. The hexanes were decanted and the solid washed with hexanes and dried in vacuum. This afforded the title compound as a white solid (86% yield). MS (apci) m/z = 442.1 (M+H).

WO 2011/006074 PCT/US2010/041538 84 Example 8 F F/ N-N KIN N N 0 NH O O OH (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-yl)-N-(1-hydroxy-2-methylpropan-2 yl)yrazolo[1,5-alpyrimidine-3-carboxamide [00454] The compound was prepared according to Example 3 using 2-amino-2 methylpropan-1-ol (4 equiv.). In this instance, the amine was added to the crude acid chloride in THF at 0 'C and the mixture was stirred for 15 hours during which time the temperature reached ambient temperature after 1-2 hours. The reaction mixture was partitioned into H 2 0 and 50% EtOAc-hexanes. The organic layer removed and the aqueous layer was extracted with 50% EtOAc-hexanes. The combined organic fractions were washed with IM NaOH, H 2 0 and saturated NaCl. The solution was dried over MgSO 4 and eluted through a SPE SiOH column eluting first with 50% EtOAc-hexanes then with EtOAc. The EtOAc pool was concentrated and residual colorless glass was dissolved in Et 2 0. Hexane was added and the resulting white suspension was concentrated to afford the title compound as a white solid (57% yield). MS (apci) m/z = 416.1 (M+H). Example 9 F / -N F N NH 0 N (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)-N-(2-methyl-1-morpholinopropan-2 Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide [004551 The title compound was prepared according to Example 4 using 2-methyl-1 morpholinopropan-2-amine (1.5 equiv). The compound was isolated as a white solid after SiO 2 chromatography using EtOAc for elution (83% yield). MS (apci) m/z = 485.2(M+H).

WO 2011/006074 PCT/US2010/041538 85 Example 10 F F *.N' N N N NH 0 \ (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-vl)-N-methylpyrazolo[1,5-alpyrimidine-3 carboxamide [00456] Prepared by the method as described in Example 1, substituting tert-butyl amine with methyl amine, to provide the final product as a white solid (34 mg, 83% yield). MS (apci) m/z = 358.1 (M+H). Example 11 F F - N O N N 0 CO 2 H (R)-1-(5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3 carbonyl)piperidine-4-carboxylic acid [004571 Step A: Preparation of (R)-ethyl 1-(5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl) pyrazole [1,5-alpyrimidine-3-carbonyl)piperidine-4-carboxvlate: Prepared by the method as described in Example 1, substituting tert-butyl amine with ethyl piperidine-4-carboxylate. The crude material was purified by preparative TLC plate, eluting first with EtOAc and then 10% MeOH/EtOAc to afford the title compound (49 mg, 88% yield). MS (apci) m/z = 484.1 (M+H). [00458] Step B: (R)-1-(5-(2-(2,5-difluorophenvll)pyrrolidin-1-Vl)pyrazolo[1,5-a pyrimidine-3-carbonyl)piperidine-4-carboxylic acid: (R)-ethyl 1-(5-(2-(2,5-di fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carbonyl)piperidine-4-carboxylate (49 mg, 0.10 mmol) was dissolved in 1:1 THF/MeOH (1.0 mL) and IM LiOH (0.20 mL, 0.20 mmol) was added. The mixture was stirred at ambient temperature for 2 hours and the reaction mixture was concentrated. The residue was diluted in water and the mixture acidified with 2N HCl. The mixture was extracted with DCM and EtOAc. The combined organics were washed with brine, dried with MgSO 4 , filtered and concentrated. The residue was triturated with hexanes and the resulting white suspension was concentrated to afford the final product (43 mg, 92 % yield) as a white solid. MS (apci) m/z = 456.1 (M+H).

WO 2011/006074 PCT/US2010/041538 86 Example 12 F /QN N N

CO

2 H 0 (R)-2-(1-(5-(2-(2,5-difluorophenvll)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3 carbonyl)piperidin-4-yl)acetic acid [00459] Step A: Preparation of (R)-ethyl 2-(1-(5-(2-(2,5-difluorophenvll)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carbonyl)piperidin-4-yl)acetate: Prepared by the method as described in Example 11, substituting ethyl piperidine-4-carboxylate with ethyl 2-(piperidin 4-yl)acetate in step A (48 mg, 83% yield). MS (apci) m/z = 498.1 (M+H). [00460] Step B: Preparation of (R)-2-(1-(5-(2-(2,5-difluorophenyl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carbonyl)piperidin-4-vl)acetic acid: Prepared as described in Example 11 Step B to afford the final product (30 mg, 66% yield) as a white solid. MS (apci) m/z = 470.1 (M+H). Example 13 F F N' QN N NH (R)-N-cyclopropyl-5-(2-(2,5-difluorophenvll)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3 carboxamide [00461] Prepared by the method as described in Example 1 substituting tert-butyl amine with cyclopropanamine. The crude material was purified by preparative TLC eluting with EtOAc then 10% MeOH/EtOAc to provide the final product as a white solid (28 mg, 63% yield). MS (apci) m/z = 384.1 (M+H). Example 14 F N'N -N H O N WO 2011/006074 PCT/US2010/041538 87 (R)-N-cyclobutyl-5-(2-(2,5-difluorophenvll)pyrrolidin- 1 -vl)pyrazolo[ 1,5-alpyrimidine-3 carboxamide [00462] Prepared by the method as described in Example 1 substituting tert-butyl amine with cyclobutanamine, to provide the final product as a white solid (41 mg, 88% yield). MS (apci) m/z = 398.1 (M+H). Example 15 F F O NH N-((2S)-bicyclo[2.2. llheptan-2-yl)-5-((R)-2-(2,5-difluorophenyl)pyrrolidin-1 vl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00463] Prepared by the method as described in Example 1, substituting tert-butyl amine with (2R)-bicyclo[2.2.1]heptan-2-amine. The crude material was purified by reverse phase chromatography eluting with 0-100% acetonitrile/water to yield the title compound as a white solid (47 mg, 92% yield.). MS (apci) m/z = 438.2 (M+H). Example 16 F F N' N NH NH 0 OH (R)-5 -(2-(2,5 -difluorophenyl)pyrrolidin- 1 -yl)-N-( 1 (hydroxymethyl)cvclopropyl)pyrazolo [ 1,5 -a]pyrimidine-3 -carboxamide [00464] Prepared by the method as described in Example 8, using (1 aminocyclopropyl)methanol (1.5 equiv.) the procedure described for Example 8. The title compound was obtained as a white solid (35% yield). MS (apci) m/z = 414.1 (M+H).

WO 2011/006074 PCT/US2010/041538 88 Example 17 F F ~:NN' N N N O NH OH (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)-N-(2-hydroxy-2-methylpropyl)pyrazolo[1,5 alpyrimidine-3-carboxamide [004651 Prepared by the method as described in Example 8, using 1-amino-2 methylpropan-2-ol (4.0 equiv.). The title compound was obtained as a white solid (62% yield). MS (apci) m/z = 416.1 (M+H). Example 18 F F - NOH cN N 0 NO'O (5-((R)-2-(2,5-difluorophenvl)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidin-3-vl)((S)-3 hydroxypyrrolidin- 1 -yl)methanone [00466] The title compound was prepared by the method as described in Example 1 using (S)-pyrrolidin-3-ol (2.0 equiv). The EtOAc pool was concentrated and the residual colorless glass was dissolved in EtOAc. Hexanes were added and resulting white suspension was concentrated to give the title compound as a white solid (42% yield). MS (apci) m/z = 414.1 (M+H). Example 19 F F N F N N .OH (5-((R)-2-(2,5-difluorophenvl)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidin-3-yl)((R)-3 hydroxypyrrolidin- 1 -yl)methanone [004671 Prepared by the method as described in Example 18 using (R)-pyrrolidin-3-ol (2.0 equiv.). The title compound was obtained as a white solid (99% yield). MS (apci) m/z = 414.1 (M+H).

WO 2011/006074 PCT/US2010/041538 89 Example 20 F F CJN N H NH 0 b 0 (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-vl)-N-(tetrahydro-2H-pyran-4-vl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00468] The title compoundg was prepared following the method of Example 1, using tetrahydro-2H-pyran-4-amine (2.0 equiv.). The EtOAc pool was concentrated and the residual colorless glass was dissolved in EtOAc. Hexanes were added and resulting white suspension was concentrated to give the title compound as a white solid (68% yield). MS (apci) m/z = 428.1 (M+H). Example 21 F F N NH N (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)-N-((1-methyl-iH-imidazol-4-yl)methyl) pyrazole[1,5-alpyrimidine-3-carboxamide [00469] Prepared by the method described in Example 1 substituting tert-butyl amine with (1-methyl-iH-imidazol-4-yl)methanamine. The crude material was purified by reverse phase chromatography, eluting with 0-100% acetonitrile/water to yield the title compound as a white solid (22 mg, 43% yield.). MS (apci) m/z = 438.1 (M+H). Example 22 F F I iN N H N (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-yl)-N-((1-methyl-iH-pyrazol-4 yl)methyl)pyrazolo[1,5-alpyrimidine-3-carboxamide WO 2011/006074 PCT/US2010/041538 90 [004701 Prepared by the method described in Example 1 substituting tert-butyl amine with (1-methyl-1H-pyrazol-4-yl)methanamine. The crude material was purified by reverse phase chromatography, eluting with 0-100% acetonitrile/water to yield the title compound as a white solid (34 mg, 67% yield.). MS (apci) m/z = 438.1 (M+H). Example 23 F F N N H NH NJ (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)-N-(2-(1-methyl-iH-imidazol-5 Vl)ethyl)pyrazolo[1,5-alpyrimidine-3-carboxamide [004711 Prepared by the method described in Example 1 substituting tert-butyl amine with 2-(1-methyl-1H-imidazol-5-yl)ethanamine. The crude material was purified by reverse phase chromatography, eluting with 0-100% acetonitrile/water to yield the title compound as a white solid (26 mg, 49% yield.). MS (apci) m/z = 452.2 (M+H). Example 24 F F N N NH 0 NH (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1 -vl)-N-(2-(2-oxoimidazolidin-1-vl)ethyl) pyrazole[1,5-alpyrimidine-3-carboxamide [00472] Prepared by the method described in Example 1, substituting tert-butyl amine with 1-(2-aminoethyl)imidazolidin-2-one. The crude material was purified by reverse phase chromatography, eluting with 0-100% acetonitrile/water to yield the title compound as a white solid (23 mg, 43% yield.). MS (apci) m/z = 456.1 (M+H).

WO 2011/006074 PCT/US2010/041538 91 Example 25 F F N NH NH (R)-N-(2-(1H-imidazol-4-yl)ethyl)-5-(2-(2,5-difluorophenyl)yrrolidin-1-yl)pyrazole [1,5 alpyrimidine-3-carboxamide [00473] Prepared by the method described in Example 1, substituting tert-butyl amine with histamine. The crude material was purified by reverse phase chromatography, eluting with 0-100% acetonitrile/water to yield the title compound as a white solid (17 mg, 34% yield.). MS (apci) m/z = 438.2 (M+H). Example 26 F F N NH O OH OH 5-((R)-2-(2,5-difluorophenvl)pyrrolidin-1-yl)-N-((R)-2,3-dihydroxvpropyl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00474] Prepared by the method described in Example 1, substituting tert-butyl amine with (R)-3-aminopropane-1,2-diol. The crude material was purified by preparative TLC using EtOAc then 10% MeOH/EtOAc for elution to afford the title compound (19 mg, 39% yield) as a white solid. MS (apci) m/z = 418.1 (M+H). Example 27 F F N cJ N N/ o \ (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1 -yl)-N,N-dimethylpyrazolo[1,5-aklyrimidine-3 carboxamide WO 2011/006074 PCT/US2010/041538 92 [004751 Prepared by the method described in Example 1 substituting tert-butyl amine with dimethylamine. The crude material was purified by preparative TLC eluting with EtOAc then 10% MeOH/EtOAc to afford the title compound (7 mg, 19% yield) as a white solid. MS (apci) m/z = 372.1 (M+H). Example 28 F F N N NH N (R)-N-(2-(1H-imidazol-1-yl)ethyl)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-Vl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00476] Prepared by the method described in Example 1 substituting tert-butyl amine with 2-(1H-imidazol-1-yl)ethanamine dihydrobromide. The crude material was purified by reverse phase chromatography eluting with 0-100% acetonitrile/water to yield the title compound as a white solid (25 mg, 57% yield.). MS (apci) m/z = 438.1 (M+H). Example 29 F F N NH O ~ OH OH 5-((R)-2-(2,5-difluorophenvll)pyrrolidin-1 -yl)-N-((S)-2,3-dihydroxvpropyl)pyrazolo[1,5 alpyrimidine-3-carboxamide [004771 A mixture of (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid (Preparation C, 400 mg, 1.16 mmol), HATU (486 mg, 1.28 mmol), and (S)-3-aminopropane-1,2-diol (318 mg, 3.49 mmol) in dry DMF (3.0 mL) was stirred for 1-2 minutes at ambient temperature. Diisopropylethylamine (DIEA) (0.62 mL, 3.49 mmol) was added and the reaction was flushed with N 2 , sealed and stirred at ambient temperature for 18 hours. The reaction mixture was added to H 2 0 (15 mL), mixed and extracted with EtOAc. The combined EtOAc extracts were washed with H 2 0, saturated NaHCO 3 and dried over MgSO 4 /activated carbon. The solution was eluted through a SiO 2 WO 2011/006074 PCT/US2010/041538 93 column eluting first with EtOAc then 10% MeOH/EtOAc. The 10% MeOH/EtOAc pool was concentrated and the residual, colorless glass was dissolved in a minimal amount of CH 2 Cl 2 . Hexane was added and the resulting white suspension was sonicated and concentrated to give the title product as a white solid (205 mg, 42%). MS (apci) m/z = 418.1 (M+H). Example 30 F F NH2 (R)-5-(2-(2,5-difluorophenyl)yrrolidin-1-yl)pyrazolo[1,5-aklyrimidine-3-carboxamide [00478] Step A: Preparation of 5-hydroxypyrazolo[1,5-a]pyrimidine-3-carbonitrile. To a mixture of 5-amino-1H-pyrazole-4-carbonitrile (2.70 g, 25.0 mmol) and Cs 2

CO

3 (16.3 g, 50.0 mmol) in dry DMF (70 mL) was added ethyl 3-ethoxyacrylate (5.41 g, 37.5 mmol) and the mixture was heated at 100 'C for 4 hours. The mixture was cooled to ambient temperature and the resultant slurry was poured into deionized H 2 0 (150 mL). The resulting aqueous solution was cooled on an ice bath and concentrated HCl was added slowly with mixing to pH = 3.5. The resulting precipitate was collected, washed with H 2 0 followed by Et 2 0. The solid was dried in vacuum to afford the product as a light beige powder (3.87 g, 97%). MS (apci) m/z = 159.0 (M-1). [004791 Step B: Preparation of (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carbonitrile. A flask was charged with the product from Step A (2.80 g, 17.5 mmol), benzotriazole-1-yl-oxy-tris-(dimethylamino)-phosphonium hexafluorophosphate (9.28 g, 21.0 mmol) and dry DMF (35 mL). The suspension was stirred at ambient temperature for 2 minutes and (R)-2-(2,5-difluorophenyl)pyrrolidine (Preparation A, 3.84 g, 21.0 mmol) and diisopropylethylamine (6.78 g, 62.5 mmol) were sequentially added (mild exotherm). The mixture was stirred at ambient temperature for 3 hours and poured into H 2 0 (175 mL). The mixture was extracted with 50% EtOAc-hexanes and the combined organic fractions were washed sequentially with IM HCl, H 2 0, IM Na 2

CO

3 and saturated NaCl. The solution was dried over MgSO 4 /activated carbon and filtered through a short Si0 2 plug (350 mL course frit funnel, 1/4 full of Si0 2 , capped with a layer of MgSO 4 ) using 50% EtOAc-hexanes for elution. The solution was concentrated to give the title WO 2011/006074 PCT/US2010/041538 94 compound as a brittle white foam that was crushed to a flowing white solid and dried in vacuum (5.50 g, 97%). MS (apci) m/z = 326.2 (M+H). [00480] Step C: Preparation of (R)-5-(2-(2,5-difluorophenyl)yrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide. The product from Step B (3.00 g, 8.85 mmol) was added in small portions over 5 minutes to concentrated H 2

SO

4 (30 mL) and the mixture was stirred at ambient temperature for 2 hours (homogeneous after 5 minutes). The solution was slowly added to chilled H 2 0 (300 mL) with stirring and the mixture was extracted with EtOAc. The combined EtOAc portions were washed with H 2 0, IM Na 2

CO

3 and saturated NaCl. The EtOAc solution was dried over MgSO 4 /activated carbon, filtered through a packed Celite pad and concentrated to give a white foam. The foam was dissolved in minimal CH 2 Cl 2 and hexane was added to induce formation of a white precipitate. The mixture was concentrated to provide the title compound as a flowing white solid after drying in vacuum (2.80 g, 92%). MS (apci) m/z = 344.1 (M+H). Example 31 F S - N' F N N OH (R)-(5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidin-3-yl)(3 hydroxyazetidin- 1 -yl)methanone [00481] The title compound was prepared according to the method of Example 1, using azetidin-3-ol hydrochloride (2.0 equiv.). In this instance, the dried EtOAc solution was eluted through a SPE SiOH column eluting first with EtOAc then with 10% MeOH-EtOAc. The MeOH-EtOAc pool was concentrated to afford the title compound as a white solid (43% yield). MS (apci) m/z = 400.0 (M+H). Example 32 F F ~ - N F N 0 N N OH (R)-(5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidin-3-yl)(3-hydroxy-3 methylazetidin- 1 -yl)methanone WO 2011/006074 PCT/US2010/041538 95 [00482] The title compound was prepared according to the method of Example 1, using 3-methyl-azetidin-3-ol trifluoroacetate (2.0 equiv.). The dried EtOAc solution was eluted through a SPE SiOH column eluting first with EtOAc then with 10% MeOH-EtOAc. The MeOH-EtOAc pool was concentrated to afford the title compound as a white solid (71% yield). MS (apci) m/z = 414.1 (M+H). Example 33 F N NH

CO

2 H Trans-4-(5-((R)-2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3 carboxamido)cyclohexanecarboxylic acid [00483] Step A: Preparation of (trans)-methyl 4-aminocyclohexanecarboxylate hydrochloride. (Trans)-4-aminocyclohexanecarboxylic acid (200 mg, 1.40 mmol) was suspended in MeOH (5.5 mL) and cooled to -10 0 C. To this was added SOCl 2 (204 gL, 2.79 mmol) dropwise and the mixture stirred for 15 minutes. The reaction mixture was warmed to ambient temperature for 15 minutes, followed by heating at reflux for 1 hour. After cooling, the mixture was concentrated to afford the title compound (260 mg, 96.1 % yield). MS (apci) m/z = 158.0 (M+H). [00484] Step B: Preparation of (Trans)-methyl 4-(5-((R)-2-(2,5 difluorophenvl)pyrrolidin-1 -Vl)pyrazolo[1,5-alpyrimidine-3 carboxamido)cyclohexanecarboxylate. Prepared by the method described in Example 1 substituting tert-butyl amine with (trans)-methyl 4-aminocyclohexanecarboxylate hydrochloride. The crude material was purified by preparative TLC using EtOAc then 10% MeOH/EtOAc for elution to afford the title compound (38 mg, 91% yield) as a colorless oil. MS (apci) m/z = 484.1 (M+H). [004851 Step C: Preparation of (trans)-4-(5-((R)-2-(2,5-difluorophenyl)pyrrolidin-1 Vl)pyrazolo[1,5-al pyrimidine-3-carboxamido)cyclohexanecarboxylic acid. Prepared by the method as described in Example 11, step B to afford the title compound (29 mg, 79% yield) as a white solid. MS (apci) m/z = 4701 (M+H).

WO 2011/006074 PCT/US2010/041538 96 Example 34 F 00

H

3 CO0 OH 6H 5-((R)-2-(5-fluoro-2-methoxyphenyl)pyrrolidin-1-yl)-N-((trans)-4 hydroxvcvclohexvl)pyrazolo[1,5-alpyrimidine-3-carboxamide [004861 Prepared by the method as described in Example 1 using (R)-5-(2-(5-fluoro-2 methoxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation G) and (trans)-4-aminocyclohexanol. The crude material was purified by reverse phase chromatography, eluting with 0-60% acetonitrile/water to yield the title compound as a white solid (32 mg, 97% yield.). MS (apci) m/z = 454.1 (M+H). Example 35 F NH 6H 5-((R)-2-(3-fluorophenvll)pyrrolidin-1-vl)-N-((trans)-4-hydroxvcvclohexvl)pyrazolo[1,5 alpyrimidine-3-carboxamide [004871 Prepared by the method as described in Example 1 using (R)-5-(2-(3 fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation E) and (trans)-4-aminocyclohexanol to yield the title compound as a white solid (31 mg, 62% yield.). MS (apci) m/z = 424.1 (M+H). Example 36 F NH (R)-N-tert-butyl-5-(2-(3-fluorophenvll)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3 carboxamide WO 2011/006074 PCT/US2010/041538 97 [00488] Prepared by the method as described in Example 1 using (R)-5-(2-(3 fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation E) to yield the title compound as a white solid (33 mg, 74% yield.). MS (apci) m/z = 382.1 (M+H). Example 37 F N N NH (R)-N-cyclopropyl-5-(2-(3-fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3 carboxamide [00489] Prepared by the method as described in Example 1 using (R)-5-(2-(3 fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation E) and cyclopropylamine to yield the title compound as a white solid (23 mg, 54% yield.). MS (apci) m/z = 366.1 (M+H). Example 38 F F / N F NH 0 / CN (R)-N-(2-cyanopropan-2-yl)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00490] Prepared by the method described in Example 1 substituting tert-butyl amine with 2-amino-2-methylpropanenitrile. The crude material was purified by preparative TLC using EtOAc then 10% MeOH/EtOAc for elution to afford the title compound (15 mg, 41% yield) as a white solid. MS (apci) m/z = 411.1 (M+H). Example 39 WO 2011/006074 PCT/US2010/041538 98 F F N I N N NH 0 \CN (R)-N-(cyanomethyl)-5-(2-(2,5-difluorophenvll)pyrrolidin- 1 -Vl)pyrazolo[ 1,5-alpyrimidine-3 carboxamide [00491] Prepared by the method described in Example 1 substituting tert-butyl amine with 2-aminoacetonitrile to provide the final product as a white solid (31 mg, 94% yield). MS (apci) m/z = 383.0 (M+H). Example 40 F F~ -N N o F (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1 -yl)-N-(1-fluoro-2-methylpropan-2-Vl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00492] Prepared by the method described in Example 1 substituting tert-butyl amine with 1-fluoro-2-methylpropan-2-amine to provide the title compound as a white solid (31 mg, 84% yield). MS (apci) m/z = 418.0 (M+H). Example 41 F G N N NH HO 0 N-cyclopropyl-5-((2R,4R)-2-(3-fluorophenvl)-4-hydroxvpyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00493] Step A: Preparation of (R)-3-(tert-butyldimethylsilvloxv)-4 chlorobutanenitrile. Tert-butyldimethylsilanecarbonitrile (20.0 g, 142 mmol), (R)-2 (chloromethyl)oxirane (13.1 g, 142 mmol) and tetrabutylammonium cyanide (0.380 g, 1.42 mmol) were mixed and heated at 100 0 C for 15 hours. After cooling, the crude mixture was concentrated and the residue purified by silica chromatography eluting with 5% WO 2011/006074 PCT/US2010/041538 99 EtOAc/hexanes to afford (R)-3-(tert-butyldimethylsilyloxy)-4-chlorobutanenitrile (17.9 g, 54%) as a clear oil. [00494] Step B: Preparation of (R)-3-(tert-butyldimethylsilyloxy)-5-(3-fluorophenyl) 3,4-dihydro-2H-pyrrole. (3-fluorophenyl)magnesium bromide (203 mL, 102 mmol, 0.5 M in ether) was slowly added via syringe to a solution of (R)-3-(tert-butyldimethylsilyloxy)-4 chlorobutanenitrile (9.50 g, 40.6 mmol) in MTBE (120 mL). The reaction was stirred for two hours and DME (35 mL) was slowly added over 15 minutes followed by EtOH (23 mL). After stirring overnight, brine (50 mL) and IM NaOH (50 mL) were added and the reaction stirred for 1 hour. The reaction mixture was filtered through a pad of Celite and the collected solids were washed with EtOAc. The filtrate was washed with IN NaOH and brine, filtered through phase-separator paper and concentrated to provide the title compound that was used directly in the next step. MS (apci) m/z = 294.2 (M+H). [004951 Step C: Preparation of (2R,4R)-4-(tert-butyldimethylsilyloxy)-2-(3-fluoro phenyl) pyrrolidine. (R)-3-(tert-butyldimethylsilyloxy)-5-(3-fluorophenyl)-3,4-dihydro-2H pyrrole (6.21 g, 21.2 mmol) was dissolved in methanol (100 mL) and AcOH (10 mL). The reaction was cooled to -78 0 C and the sodium borohydride (2.00 g, 52.9 mmol) was slowly added in small portions. The reaction was allowed to warm to ambient temperature overnight. The reaction mixture was concentrated and the residue was diluted with EtOAc and IN NaOH. Additional NaOH pellets were added to basify the aqueous layer. The organic layer was separated and the aqueous layer was extracted with EtOAc. The combined organic layers were dried with MgSO 4 , filtered and concentrated. The residual oil was purified by silica chromatography eluting with 5% MeOH/EtOAc to afford (2R,4R)-4-(tert butyldimethylsilyloxy)-2-(3-fluorophenyl)pyrrolidine (4.82 g, 77.1 %) as a brown oil. MS (apci) m/z = 296.1 (M+H). [00496] Step D: Preparation of ethyl 5-((2R,4R)-4-(tert-butyldimethylsilvloxv)-2-(3 fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylate. Prepared according to the method of Preparation C, using (2R,4R)-4-(tert-butyldimethylsilyloxy)-2-(3 fluorophenyl)pyrrolidine in Step A. MS (apci) m/z = 485.1 (M+H). [004971 Step E: Preparation of 5-((2R,4R)-2-(3-fluorophenvl)-4-hydroxypyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. Ethyl 5-((2R,4R)-4-(tert-butyldimethyl silyloxy)-2-(3-fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (205 mg, 0.422 mmol) was suspended in EtOH (2.0 mL) and IM LiOH (0.845 ml, 0.845 mmol) was added. The mixture was heated at reflux for 4 hours and another portion of IM LiOH WO 2011/006074 PCT/US2010/041538 100 (0.845 ml, 0.845 mmol) was added. The mixture was heated at reflux overnight, cooled to ambient temperature and concentrated. The residue was diluted in water and the mixture was treated with 2N HCl to achieve pH 1. The mixture was extracted with DCM and EtOAc and the combined extracts were dried with MgSO 4 , filtered and concentrated to afford 5 ((2R,4R)-2-(3-fluorophenyl)-4-hydroxypyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxylic acid (124 mg, 86%) as a light orange solid. [00498] Step F: Preparation of N-cyclopropyl-5-((2R,4R)-2-(3-fluorophenyl)-4 hydroxypyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3-carboxamide. Prepared by the method described in Example 1 using 5-((2R,4R)-2-(3-fluorophenyl)-4-hydroxypyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid and substituting tert-butyl amine with cyclopropylamine to provide the final product as a white solid (15 mg, 66% yield). MS (apci) m/z = 382.1 (M+H). Example 42 F NNN CY N~ NH aNH HO N-tert-butyl-5-((2R,4R)-2-(3-fluorophenvl)-4-hydroxvpyrrolidin-1-Vl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00499] Prepared by the method described in Example 1 using 5-((2R,4R)-2-(3 fluorophenyl)-4-hydroxypyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid and tert-butyl amine to provide the final product as a white solid (24 mg, 100% yield). MS (apci) m/z = 398.1 (M+H). Example 43 F NH HO 5-((2R,4R)-2-(3-fluorophenyl)-4-hydroxypyrrolidin-1-yl)-N-methylpyrazolo[1,5 alpyrimidine-3-carboxamide WO 2011/006074 PCT/US2010/041538 101 [005001 Prepared by the method described in Example 1 using 5-((2R,4R)-2-(3 fluorophenyl)-4-hydroxypyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid and methylamine to provide the final product as a white solid (9.4 mg, 45% yield). MS (apci) m/z= 356.1 (M+H). Example 44 F F C N N N NH 0 b S02CH 3 (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-yl)-N-(1-(methylsulfonyl)piperidin-4 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [005011 Prepared by the method described in Example 1 using 1 (methylsulfonyl)piperidin-4-amine hydrochloride (1.5 equiv.). The title compound was isolated as a white solid (83% yield) after purification by SiO 2 column (eluting with 50% EtOAc-hexanes, then EtOAc, and then 10% MeOH-EtOAc). MS (apci) m/z = 505.0 (M+H). Example 45 F NN N N N NH 0b N

SO

2

NH

2 (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)-N-(1-sulfamoylpiperidin-4-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00502] Prepared by the method described in Example 1 using 4-aminopiperidine-1 sulfonamide (1.5 equiv.). The title compound was isolated as a white solid (80% yield) after SiO 2 column purification (eluting with 50% EtOAc-hexanes, then EtOAc, then 10% MeOH EtOAc). MS (apci) m/z = 506.0 (M+H). Example 46 WO 2011/006074 PCT/US2010/041538 102 F -N F N N NH 0

NHSO

2

CH

3 (R)-5 -(2-(2,5-difluorophenvll)pyrrolidin- 1 -vl)-N-(2-(methylsulfonamido)ethyl)pyrazolo[ 1,5 alpyrimidine-3-carboxamide [00503] Prepared by the method described in Example 1 using N-(2 aminoethyl)methanesulfonamide hydrochloride (2.0 equiv.). The title compound was isolated as a white solid (67% yield) after SiO 2 column purification (eluting with 50% EtOAc hexanes, then EtOAc, then 10% MeOH-EtOAc). MS (apci) m/z = 465.0 (M+H). Example 47 F N-N F N'j N N NH 0

SO

2 NH2 (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)-N-(2-sulfamoylethyl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00504] Prepared by the method described in Example 1 using 2 aminoethanesulfonamide (2.0 equiv.). The title compound was isolated as a white solid (67% yield) after SiO 2 column purification (eluting with 50% EtOAc-hexanes, then EtOAc, then 10% MeOH-EtOAc). MS (apci) m/z = 451.0 (M+H). Example 48 F

H

3 CO ~ N N NH (R)-N-cyclopropyl-5-(2-(5-fluoro-2-methoxvphenvl)pyrrolidin-1-vl)pyrazolo[1,5 alpyrimidine-3-carboxamide [005051 Prepared by the method described in Example 1 using (R)-5-(2-(5-fluoro-2 methoxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation G) WO 2011/006074 PCT/US2010/041538 103 and cyclopropylamine to yield the title compound as a white solid (19 mg, 68% yield). MS (apci) m/z = 396.0 (M+H). Example 49 F

H

3 CO N O NH OH (R)-5-(2-(5-fluoro-2-methoxyphenyl)pyrrolidin-1-yl)-N-(2-hydroxy-2 methylpropyl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00506] Prepared by the method described in Example 1 using (R)-5-(2-(5-fluoro-2 methoxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation G) and 1-amino-2-methylpropan-2-ol to yield the title compound as a white solid (17 mg, 55% yield). MS (apci) m/z = 428.1 (M+H). Example 50 F N HO 5-((R)-2-(2,5-difluorophenvll)pyrrolidin-1-vl)-N-(4-hydroxy-4-methyl cyclohexyl)pyrazolo[1,5-alpyrimidine-3-carboxamide (Diastereomer 1) [005071 Step A: Preparation of diastereomeric tert-butvl-4-hydroxy-4 methylcyclohexyl carbamates. A solution of tert-butyl 4-oxocyclohexylcarbamate (1.20 g, 5.63 mmol) in dry THF (28.1 mL, 5.63 mmol) was cooled to -78 0 C and 3.0 M MeMgCl (5.72 mL, 17.2 mmol) was added. The reaction mixture was allowed to warm to ambient temperature and stirred for 48 hours. The reaction was quenched with saturated NH 4 Cl (10 mL) and concentrated in vacuo. The residue was diluted in water and DCM and solid citric acid was added until the phases separated. The organic layer was removed and washed with saturated NaHCO 3 , water and brine. The solution was dried with MgSO 4 filtered and concentrated to give a mixture of diastereomeric products as a white solid. The two diastereomers were separated using silica chromatography eluting with a gradient of 20-80% WO 2011/006074 PCT/US2010/041538 104 EtOAc/Hexanes: Minor isomer (45.1 mg, 7% yield), major isomer (113 mg, 18% yield). MS (apci) m/z = 130.0 (M+H - Boc). [00508] Step B: Preparation of 5-((R)-2-(2,5-difluorophenyl)pyrrolidin-1-yl)-N-(4 hydroxy-4-methylcvclohexvl)pyrazolo[1,5-alpyrimidine-3-carboxamide (Diasteromer 1). The minor isomer from Step A (45.1 mg, 0.197 mmol) was dissolved in DCM (1.0 mL) and 4N HCl in dioxane (492 gL, 1.97 mmol) was added. The reaction mixture was stirred at ambient temperature for 1 hour and was concentrated to afford 4-amino-i -methylcyclohexanol (minor isomer). The 4-amino-1-methylcyclohexanol was reacted with (R)-5-(2-(2,5 difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation C) according to the procedure outlined in Example 1 to provide the title product as a white solid (14 mg, 48% yield). MS (apci) m/z = 456.1 (M+H). Example 51 F F FN NNH OH 5-((R)-2-(2,5-difluorophenvll)pyrrolidin-1-vl)-N-(4-hydroxy-4 methylcyclohexyl)pyrazolo[1,5-alpyrimidine-3-carboxamide (Diasteromer 2) [005091 The major isomer from Step A in Example 50 (45.1 mg, 0.197 mmol) was dissolved in DCM (1.0 mL) and 4N HCl in dioxane (492 gL, 1.97 mmol) was added. The reaction mixture was stirred at ambient temperature for 1 hour and was concentrated to afford 4-amino-1-methylcyclohexanol (major isomer). The 4-amino-1-methylcyclohexanol was reacted with (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxylic acid (Preparation C) according to the procedure outlined in Example 1 to provide the title product as a white solid (10.7 mg, 38% yield). MS (apci) m/z = 456.1 (M+H). Example 52 F N S NN NH Ci 0 WO 2011/006074 PCT/US2010/041538 105 (R)-N-cyclopropyl-5-(2-(5-fluoropyridin-3-yl)pyrrolidin- 1 -yl)pyrazolo[ 1,5-alpyrimidine-3 carboxamide [005101 To a solution of (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid (Preparation I, 30.0 mg, 0.092 mmol) and HATU (52.1 mg, 0.137 mmol) in dry DMF (0.5 mL) was added cyclopropylamine (10.5 mg, 0.183 mmol) followed by diisopropylethylamine (35.5 mg, 0.275 mmol). The mixture was stirred under an atmosphere of N 2 for 43 hours. The crude mixture was purified by reverse phase chromatography eluting with 0-50% acetonitrile/water to yield the title compound as a white solid (26 mg, 78% yield). MS (apci) m/z = 367.0 (M+H). Example 53 F N N NH 0 \ (R)-N-tert-butyl-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1 -yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [005111 Prepared by the method as described in Example 1 using (R)-5-(2-(5 fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation I) and 2-methylpropan-2-amine to yield the title compound as a white solid (23 mg, 67% yield). MS (apci) m/z = 383.1 (M+H). Example 54 F N NH NH HCO N 0 (R)-5-(2-(5-fluoro-2-methoxyphenyl)pyrrolidin-1-yl)-N-(2-morpholinoethyl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00512] Prepared by the method as described in Example 4, using (R)-5-(2-(5-fluoro 2-methoxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation WO 2011/006074 PCT/US2010/041538 106 G) and 2-morpholinoethanamine (1.5 equiv.). The title compound was obtained as a white solid (65% yield). MS (apci) m/z = 469.1 (M+H). Example 55 F

H

3 C O N O N N OH OH N-((S)-2,3-dihydroxypropyl)-5-((R)-2-(5-fluoro-2-methoxyphenyl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00513] Prepared by the method of Example 1, using (R)-5-(2-(5-fluoro-2 methoxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation G) and (S)-3-aminopropane-1,2-diol (2.0 equiv). The crude material was purified by SiO 2 column chromatography, eluting with EtOAc then 10% MeOH-EtOAc to afford the title compound as a white solid (53% yield). MS (apci) m/z = 430.1 (M+H). Example 56 F

H

3 CO N N NH NH O OH OH N-((R)-2,3-dihydroxvpropyl)-5-((R)-2-(5-fluoro-2-methoxvphenvll)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00514] Prepared by the method of Example 1, using (R)-5-(2-(5-fluoro-2 methoxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation G) and (R)-3-aminopropane-1,2-diol (2.0 equiv). The crude material was purified by SiO 2 column chromatography, eluting with EtOAc then 10% MeOH-EtOAc to afford the title compound as a white solid (46% yield). MS (apci) m/z = 430.1 (M+H). Example 57 WO 2011/006074 PCT/US2010/041538 107 F F ~!N N N N NH N H SO 2

CH

3 (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-vl)-N-(2-methyl-1-(methylsulfonamido)propan-2 yl)yrazolo[1,5-alpyrimidine-3-carboxamide [005151 Step A: Preparation of tert-butyl 2-amino-2-methylpropylcarbamate. Tert butyl phenyl carbonate (0.421 mL, 2.270 mmol) was added to a solution of 2-methylpropane 1,2-diamine (200 mg, 2.270 mmol) in EtOH (4.5 mL) and the reaction mixture was heated at reflux overnight. The mixture was concentrated and the residue diluted in water. The mixture was acidified with 2N HCl to pH 4 and washed with DCM. The aqueous layer was treated with IM NaOH (2 mL) and extracted with DCM. The combined organic layers were dried with MgSO 4 , filtered and concentrated to afford tert-butyl 2-amino-2 methylpropylcarbamate (158 mg, 37 % yield) as a colorless oil. MS (apci) m/z = 188.9 (M+H). [00516] Step B: Preparation of (R)-tert-butvl 2-(5-(2-(2,5-difluorophenvllpyrrolidin 1-yl)pyrazolo[1,5-a]lpyrimidine-3-carboxamido)-2-methylpropylcarbamate. Prepared by the method described in Example 1 using tert-butyl 2-amino-2-methylpropylcarbamate to provide the title compound as a colorless oil (109 mg, 100% yield). MS (apci) m/z = 515.2 (M+H). [005171 Step C: Preparation of (R)-N-(1-amino-2-methylpropan-2-yl)-5-(2-(2,5 difluorophenvl)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide hydrochloride. (R)-tert-butyl 2-(5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxamido)-2-methylpropylcarbamate (109 mg, 0.212 mmol) was dissolved in DCM (1.0 mL) and 4N HCl in dioxane (0.530 mL, 2.12 mmol) was added. The mixture was stirred at ambient temperature for 4 hours and was concentrated afford the title compound (105 mg). MS (apci) m/z = 415.2 (M+H). [00518] Step D: Preparation of (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)-N-(2 methyl -1-(methylsulfonamido)propan-2-Vl)pyrazolo[1,5-a]pyrimidine-3-carboxamide. (R) N-(1-amino-2-methylpropan-2-yl)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a] pyrimidine-3-carboxamide hydrochloride (24.0 mg, 0.0532 mmol) was dissolved in DCM (0.53 mL) and triethylamine (15.2 gL, 0.109 mmol) followed by MeSO 2 Cl (4.34 gL, 0.0559 WO 2011/006074 PCT/US2010/041538 108 mmol) were added sequentially. The mixture was stirred at ambient temperature for 2 hours and was diluted with EtOAc. The mixture was washed with water and brine and was dried with MgSO 4 . The solution was filtered and concentrated to afford the title compound (8.0 mg, 30 % yield) as a white solid. MS (apci) m/z = 493.1 (M+H). Example 58 F N F N Kli N NH 0

NH

2 (R)-N-(2-amino-2-methylpropyl)-5-(2-(2,5-difluorophenvll)pyrrolidin- 1 -Vl)pyrazolo[ 1,5 alpyrimidine-3-carboxamide [005191 Prepared by the method described in Example 1, using 2-methylpropane-1,2 diamine. The crude product was purified by reverse phase chromatography eluting with 0 100% acetonitrile/water to yield the title compound as a white solid (3.9 mg, 6.0% yield). MS (apci) m/z = 415.1 (M+H). Example 59 F N' NH F F (R)-N-tert-butyl-5-(4,4-difluoro-2-(3-fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00520] Step A: Preparation of (R)-N-tert-butyl-5-(2-(3-fluorophenyl)-4 oxopyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3-carboxamide: N-tert-butyl-5-((2R,4R)-2-(3 fluorophenyl)-4-hydroxypyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide (Example 42, 10 mg, 0.025 mmol) and the Dess-Martin reagent (16 mg, 0.038 mmol) in DCM (2.0 mL) were stirred at ambient temperature overnight. IN NaOH (2.5 mL) was added and the reaction stirred for 30 minutes. Brine (2.5 mL) was added and the reaction was filtered through a phase separator frit, washing with several portions of DCM. The DCM solution was concentrated and the residue purified by reverse phase chromatography (20-70% WO 2011/006074 PCT/US2010/041538 109 acetonitrile/water) to provide the title compound (2.7 mg, 27 % yield) as a clear oil. MS (apci) m/z = 396.0 (M+H). [00521] Step B: Preparation of (R)-N-tert-butyl-5-(4,4-difluoro-2-(3 fluorophenvl)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide: (R)-N-tert-butyl-5 (2-(3-fluorophenyl)-4-oxopyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide (1.40 mg, 3.54 gmol) and bis-(2-methoxyethyl)aminosulfur trifluoride (1.57 mg, 7.08 gmol) were mixed in DCM (2.0 mL) and the reaction was stirred at ambient temperature overnight. IN NaOH (1.0 mL) was added and the reaction was stirred for 30 minutes. Brine (1.0 mL) was added and the mixture was filtered through a phase separator frit, washing with several portions of DCM. The DCM solution was concentrated and the residue purified by reverse phase chromatography (0-70% acetonitrile/water) to provide the title compound (1.30 mg, 88.0 % yield) as a white solid. MS (apci) m/z = 418.1 (M+H). Example 60 F F hbN N N NH 0 \OH OH (R)-5-(2-(2,5-difluorophenvl)pyrrolidin-1-yl)-N-(1,3-dihydroxy-2-methylpropan-2 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00522] Prepared according to the method of Example 1, using 2-amino-2 methylpropane-1,3-diol (2.0 equiv). The crude material was purified by Si0 2 column chromatography, eluting with EtOAc and then 10% MeOH-EtOAc to provide the title compound as a white solid (54% yield). MS (apci) m/z = 432.1 (M+H). Example 61 F F N' N O5F N H 5-((R)-2-(2,5-difluorophenyl)pyrrolidin-1 -yl)-N-((3S,4R)-3-fluoropiperidin-4 Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide hydrochloride WO 2011/006074 PCT/US2010/041538 110 [00523] Step A: Preparation of (3S,4R)-tert-butyl 4-(5-((R)-2-(2,5 difluorophenvl)pyrrolidin- 1 -Vl)pyrazolo [ 1,5 -alpyrimidine-3 -carboxamido)-3 fluoropiperidine-1-carboxylate. Prepared according to the method of Example 1, using (3S,4R)-tert-butyl 4-amino-3-fluoropiperidine-1-carboxylate (1.5 equiv). The title compound was obtained as a white solid (79% yield). MS (apci) m/z = 545.21 (M+H). [00524] Step B: Preparation of 5-((R)-2-(2,5-difluorophenvll)pyrrolidin-1-yl-N ((3S,4R)-3-fluoropiperidin-4-yl)pyrazolo[1,5-a]yyrimidine-3-carboxamide hydrochloride. To a solution of the title compound from Step A (50.0 mg, 0.092 mmol) in EtOAc (1.5 mL) was added 4M HCl in dioxane (0.460 mL, 1.85 mmol) and the mixture was stirred at ambient temperature for 6 hours (white precipitate formed). The mixture was diluted with dry Et 2 0 (2 volumes) and sonicated to afford a fine white suspension. The solid was collected, washed with dry Et 2 0 and dried under vacuum to give the title compound as a white solid (42 mg, 95% yield). MS (apci) m/z = 445.1 (M+H). Example 62 F

F

3 C <j NH H '- uOH N-((S)-2,3-dihydroxypropyl)-5-((R)-2-(5-fluoro-2-(trifluoromethyl)phenyl) pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3-carboxamide [005251 Prepared by the method described in Example 1 using (R)-5-(2-(5-fluoro-2 (trifluoromethyl)phenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation M) and (S)-3-aminopropane-1,2-diol. The crude material was purified by reverse phase HPLC ( 0

-

6 0 % acetonitrile/water) to provide the title compound (26 mg, 73% yield). MS (apci) m/z = 468.1 (M+H). Example 63 F

F

3 C N tjN N N O OH

OH

WO 2011/006074 PCT/US2010/041538 111 N-((R)-2,3-dihydroxvpropyl)-5-((R)-2-(5-fluoro-2-(trifluoromethyl)phenvl) pyrrolidin- 1 -yl)pyrazolo[ 1,5-aklyrimidine-3-carboxamide [00526] Prepared by the method described in Example 1 using (R)-5-(2-(5-fluoro-2 (trifluoromethyl)phenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation M) and (R)-3-aminopropane-1,2-diol. The crude material was purified by reverse phase HPLC (0-60% acetonitrile/water) to provide the title compound (34 mg, 73% yield). MS (apci) m/z = 468.1 (M+H). Example 64 F F -N

F

3 C FC

NH

2 (R)-5-(2-(5-fluoro-2-(trifluoromethyl)phenvll)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3 carboxamide [005271 Prepared by the method described in Example 1 using (R)-5-(2-(5-fluoro-2 (trifluoromethyl)phenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation M) and ammonium chloride. The crude material was purified by reverse phase HPLC (0-60% acetonitrile/water) to yield the title compound (23 mg, 78% yield.). MS (apci) m/z = 394.0 (M+H). Example 65 F /~-N FNH 0 N0 0 (R)-5-(2-(2,5-difluorophenvll)pyrrolidin-1-vl)-N-methoxvpyrazolo[1,5-alpyrimidine-3 carboxamide [00528] Prepared by the method described in Example 1 using 0 methylhydroxylamine hydrochloride (2.0 equiv). The title compound was obtained as a white solid (53% yield). MS (apci) m/z = 374.1 (M+H). Example 66 WO 2011/006074 PCT/US2010/041538 112 F F NH 0 N 0 (R)-N-(cyclopropylmethoxy)-5-(2-(2,5-difluorophenyl)yrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00529] Prepared by the method described in Example 1 using 0 (cyclopropylmethyl)hydroxylamine (2.0 equiv). The title compound was obtained as a white solid (31 % yield). MS (apci) m/z = 414.1 (M+H). Example 67 F N NH2 (R)-5-(5-(2,5-difluorophenvl)-2,2-dimethylpyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3 carboxamide [00530] Step A: Preparation of (R)-tert-butyl 5-(2,5-difluorophenyl)-2,2-dimethyl pyrrolidine-1-carboxylate. Prepared by the method described in Preparation A, Step A substituting tert-butyl pyrrolidine-1-carboxylate with tert-butyl 2,2-dimethylpyrrolidine-1 carboxylate to provide the title compound as a white solid (640 mg, 37% yield). MS (apci) m/z = 212.1 (M+H - Boc). [00531] Step B: Preparation of (R)-5-(2,5-difluorophenvl)-2,2-dimethylpyrrolidine hydrochloride. Prepared by the method as described in Preparation A, Step B, using (R)-tert butyl 5-(2,5-difluorophenyl )-2,2-dimethyl pyrrolidine-1-carboxylate to afford the title compound (420 mg, 97% yield). MS (apci) m/z = 212.1 (M+H). [00532] Step C: Preparation of (R)-ethyl 5-(5-(2,5-difluorophenYl)-2,2 dimethylpyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3-carboxylate. A sealed pressure tube was charged with (R)-5-(2,5-difluoro phenyl)-2,2-dimethylpyrrolidine HCl salt (300 mg, 1.21 mmol), diisopropylethylamine (423 gl, 2.42 mmol), ethyl 5-chloropyrazolo[1,5-a]pyrimidine 3-carboxylate (273 mg, 1.21 mmol) and isopropanol (2.0 mL). The tube was sealed and the mixture was heated at 160 0 C for 3 days. Additional ethyl 5-chloropyrazolo[1,5- WO 2011/006074 PCT/US2010/041538 113 a]pyrimidine-3-carboxylate (273 mg, 1.21 mmol) was added and the reaction was heated at 160 0 C 2 days. The reaction mixture was concentrated and the residue purified by reverse phase HPLC (eluting with 0-60% acetonitrile/H 2 0) to provide the title compound (136 mg, 28%) as a beige solid. MS (apci) m/z = 401.1 (M+H). [00533] Step D: Preparation of (R)-5-(5-(2,5-difluorophenyl)-2,2-dimethylpyrrolidin 1-Vl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. (R)-ethyl 5-(5-(2,5-difluorophenyl)-2,2 dimethylpyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (136 mg, 0.340 mmol) was dissolved in MeOH (5.0 mL) and IN NaOH (3.40 mL, 3.40 mmol) was added. The reaction was stirred at ambient temperature for 5 days and then heated at reflux for 4 hours. The reaction mixture was cooled, poured onto a mixture of brine (10 mL) and 2N HCl (5 mL) and extracted with DCM. The combined organic extracts were filtered through PS paper and concentrated to provide the title compound (123 mg, 97% yield) as a beige solid. MS (apci) m/z = 373.0 (M+H). [00534] Step E: Preparation of (R)-5-(5-(2,5-difluorophenvl)-2,2-dimethylpyrrolidin 1-yl)yrazolo[1,5-a]lyrimidine-3-carboxamide. Prepared by the method described in Example 1 using (R)-5-(5-(2,5-difluorophenyl)-2,2-dimethylpyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid and ammonium chloride. The crude material was purified by reverse phase HPLC (0-70% acetonitrile/water) to provide the title compound (8.5 mg, 33% yield.). MS (apci) m/z = 372.1 (M+H). Example 68 F F N N NH (R)-N-cyclopropyl-5-(5-(2,5-difluorophenyl)-2,2-dimethylpyrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [005351 Prepared by the method described in Example 1 using (R)-5-(5-(2,5 difluorophenyl)-2,2-dimethylpyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid and cyclopropylamine in Step D. The crude material was purified by reverse phase HPLC (0-75% acetonitrile/water) to provide the title compound (11 mg, 39% yield.). MS (apci) m/z = 412.1 (M+H).

WO 2011/006074 PCT/US2010/041538 114 Example 69 F N\ N-N NH CN (R)-N-(2-cyanopropan-2-yl)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00536] Prepared by the method described in Example 1 using (R)-5-(2-(5 fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation I) and 2-amino-2-methylpropanenitrile to yield the title compound as a white solid (21 mg, 57% yield). MS (apci) m/z = 394.1 (M+H). Example 70 F NN N-N N NH 0 N

SO

2

CH

3 (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)-N-(1-(methylsulfonyl)piperidin-4 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [005371 Prepared by the method as described in Example 1 using (R)-5-(2-(5 fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation I) and 1-(methylsulfonyl)piperidin-4-amine to yield the title compound as a white solid (44 mg, 100% yield). MS (apci) m/z = 488.1 (M+H). Example 71 F N N- NH N F (R)-N-(1-fluoro-2-methylpropan-2-vl)-5-(2-(5-fluoropyridin-3-Vl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide WO 2011/006074 PCT/US2010/041538 115 [00538] Prepared by the method described in Example 1 using (R)-5-(2-(5 fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation I) and 1-fluoro-2-methylpropan-2-amine to yield the title compound as a white solid (37 mg, 100% yield). MS (apci) m/z = 401.0 (M+H). Example 72 F N N N NH 0b 0 (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)-N-(tetrahydro-2H-pyran-4-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00539] Prepared by the method as described in Example 1 using (R)-5-(2-(5 fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation I) and tetrahydro-2H-pyran-4-amine to yield the title compound as a white solid (34 mg, 90% yield). MS (apci) m/z = 411.1 (M+H). Example 73 F N -N NH 0 (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)-N-methoxypyrazolo[1,5-alpyrimidine-3 carboxamide [00540] Prepared by the method as described in Example 1 using (R)-5-(2-(5 fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation I) and O-methylhydroxylamine to yield the title compound as a white solid (15 mg, 35% yield). MS (apci) m/z = 357.0 (M+H). Example 74 F - NH 2 0 WO 2011/006074 PCT/US2010/041538 116 (R)-5-(2-(3-fluorophenvll)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00541] To a suspension of (R)-5-(2-(3-fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid (Preparation E, 50.0 mg, 0.153 mmol) in CCl 4 (1.0 mL) was added thionyl chloride (182 mg, 1.53 mmol) and the mixture was heated at reflux for 4 hours (homogeneous after 5 minutes). The mixture was cooled to ambient temperature and was concentrated to give a brittle foam. The foam was dissolved in dry THF (2 mL) and dimethylaminopyridine (DMAP) (3.74 mg, 0.031 mmol) was added. Anhydrous ammonia was bubbled into the mixture with stirring for 5 minutes. The reaction vessel was sealed and the reaction was stirred at ambient temperature for 18 hours. The mixture was added to H 2 0 (4 mL) and extracted with EtOAc. The combined extracts were washed with IM Na 2

CO

3 ,

H

2 0 and saturated NaCl. The solution was dried over MgSO 4 /activated carbon and filtered through a SiO 2 plug (EtOAc then 10% MeOH/EtOAc for elution). The solution was concentrated to give the title compound as a white solid (38 mg, 76%). MS (apci) m/z = 326.0 (M+H). Example 75 F N - N NH 0 ((R)-5-(2-(3-fluorophenyl)pyrrolidin-1-yl)-N-methoxypyrazolo[1,5-alpyrimidine-3 carboxamide [00542] To a suspension of (R)-5-(2-(3-fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid (Preparation E, 50.0 mg, 0.153 mmol) in CCl 4 (1.5 mL) was added thionyl chloride (182 mg, 1.53 mmol) and mixture heated at reflux for 4 hours (homogeneous). The mixture was cooled to ambient temperature and was concentrated to a brittle beige foam. DMAP (3.7 mg, 0.031 mmol), methylhydroxyl amine HCl (38.4 mg, 0.460 mmol) and dry THF (2 mL) were added and mixed. Diisopropylethylamine (79.2 mg, 0.613 mmol) was added, and the reaction flushed with N 2 and stirred at ambient temperature for 18 hours. The mixture was diluted with H 2 0 (4 mL) and extracted with EtOAc and the combined extracts were washed with IM Na 2

CO

3 , H 2 0 and saturated NaCl. The solution was dried over MgSO 4 /activated carbon and filtered through a SiO 2 plug eluting with EtOAc. The mixture was concentrated to give a white foam that was dissolved in minimal CH 2 Cl 2 WO 2011/006074 PCT/US2010/041538 117 and treated with dry hexanes to give a fine white suspension. The mixture was concentrated to give the title compound as white solid (42 mg, 77%). MS (apci) m/z = 356.0 (M+H). Example 76 F / N N 0O

NH

2 (R)-5-(2-(3-fluoro-5-(2-morpholinoethoxy)phenyl)pyrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00543] Step A: Preparation of (R)-tert-butyl 2-(3-fluoro-5 hydroxyphenyl)pyrrolidine-1-carboxylate. Prepared by the method as described in Preparation A, Step A, substituting 2-bromo-1,4-difluorobenzene with 3-bromo-5 fluorophenyl acetate to afford the title compounds (10.3 g, 62% yield). MS (apci) m/z = 182.1 (M+H - Boc). [00544] Step B: Preparation of (R)-3-fluoro-5-(pyrrolidin-2-yl)phenol hydrochloride. To a solution of (R)-tert-butyl 2-(3-fluoro-5-hydroxyphenyl)pyrrolidine-1 carboxylate (10.3 g, 36.5 mmol) in DCM (20 mL) was added 4N HCl in dioxane (36.5 mL, 146 mmol) and the mixture was stirred at ambient temperature for 15 hours. The resulting precipitate was filtered and washed with DCM to afford (R)-3-fluoro-5-(pyrrolidin-2 yl)phenol hydrochloride (5.81 g, 73.3 % yield). [005451 Step C: Preparation of (R)-ethyl 5-(2-(3-fluoro-5-hydroxyphenyl)pyrrolidin 1-Vl)pyrazolo[l,5-alpyrimidine-3-carboxylate. Prepared by the method as described in Preparation C, Step A, using (R)-2-(2,5-difluorophenyl)pyrrolidine and (R)-3-fluoro-5 (pyrrolidin-2-yl)phenol hydrochloride. The crude material was purified by reverse phase HPLC (0-60% acetonitrile/water) to provide yield the title compound (775 mg, 94% yield). MS (apci) m/z = 370.9 (M+H). [00546] Step D: Preparation of (R)-ethyl 5-(2-(3-fluoro-5-(2-morpholinoethoxy) phenyl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylate. (R)-ethyl 5-(2-(3-fluoro -5 hydroxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (167 mg, 0.451 mmol), 4-(2-chloroethyl)morpholine hydrochloride (168 mg, 0.902 mmol), and K 2 C0 3 (312 mg, 2.25 mmol) were suspended in DMF (5 mL) and stirred at ambient temperature for 15 hours. The crude reaction mixture was purified by reverse phase HPLC (0-60% WO 2011/006074 PCT/US2010/041538 118 acetonitrile/water) to provide the title compound (218 mg, 100% yield). MS (apci) m/z = 484.1 (M+H). [005471 Step E: Preparation of (R)-5-(2-(3-fluoro-5-(2-morpholinoethoxy)phenyl) pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. Prepared using the hydrolysis conditions described in Preparation C, Step B. The crude material was purified by reverse phase HPLC (0-40% acetonitrile/water) to yield the title compound (208 mg, 94% yield). MS (apci) m/z = 456.1 (M+H). [00548] Step F: Preparation of (R)-5-(2-(3-fluoro-5-(2-morpholinoethoxv)phenvl) pyrrolidin -1-yl)pyrazolo[1,5-alpyrimidine-3-carboxamide. Prepared by the method described in Example 1 using (R)-5-(2-(3-fluoro-5-(2-morpholinoethoxy)phenyl) pyrrolidin 1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid ammonium chloride to yield the title compound as a white solid (19 mg, 69% yield.). MS (apci) m/z = 455.1 (M+H). Example 77 F 0 N N o NH (R)-N-cyclopropyl-5-(2-(3-fluoro-5-(2-methoxyethoxy)phenyl)pyrrolidin-1-yl)pyrazole [1,5 alpyrimidine-3-carboxamide [00549] Step A: Preparation of (R)-ethyl 5-(2-(3-fluoro-5-(2-methoxyethoxy )phenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate. (R)-ethyl 5-(2-(3-fluoro-5 hydroxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (Example 76 Step B, 174 mg, 0.470 mmol), 1-bromo-2-methoxyethane (196 mg, 1.41 mmol), and K 2 C0 3 (325 mg, 2.35 mmol) were suspended in DMF (5 mL) and stirred at ambient temperature for 15 hours. The crude reaction mixture was purified by reverse phase HPLC ( 0

-

6 0 % acetonitrile/water) to provide yield the title compound (183 mg, 91% yield). MS (apci) m/z = 429.0 (M+H). [005501 Step B: Preparation of (R)-5-(2-(3-fluoro-5-(2 methoxyethoxy)phenyl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. (R) ethyl 5-(2-(3-fluoro-5-(2-methoxy ethoxy)phenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine 3-carboxylate (178 mg, 0.415 mmol) was suspended in a mixture of IN NaOH (5 mL) and MeOH (5 mL). The reaction mixture was stirred at ambient temperature until complete and WO 2011/006074 PCT/US2010/041538 119 quenched with 2N HCl (25 mL). The mixture was extracted with ethyl acetate and the combined organic fractions were concentrated to give the title compound (177 mg, 100 % yield). MS (apci) m/z = 401.0 (M+H). [005511 Step C: Preparation of (R)-N-cyclopropyl-5-(2-(3-fluoro-5-(2 methoxyethoxy) phenyl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxamide. Prepared by the method described in Example 1 using (R)-5-(2-(3-fluoro-5-(2 methoxyethoxy)phenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid and cyclopropylamine to yield the title compound as a white solid (16 mg, 52% yield). MS (apci) m/z = 440.1 (M+H). Example 78 F 0N N NH 2 (R)-5-(2-(3-fluoro-5-(2-methoxvethoxv)phenvll)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3 carboxamide [00552] Prepared by the method described in Example 77 using ammonium chloride in Step C. The crude material was purified by reverse phase HPLC ( 0

-

6 0 % acetonitrile/water) to provide the title compound (16 mg, 53% yield.). MS (apci) m/z = 400.1 (M+H). Example 79 F N N

H

3 CO QNN (R)-N-cyclopropyl-5-(2-(5-fluoro-2-methoxvpyridin-3-vl)pyrrolidin-1-Vl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00553] Prepared by the method described in Example 1 using (R)-5-(2-(5-fluoro-2 methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation K) and cyclopropanamine. The combined organic extracts were concentrated and the residue was purified by reverse phase HPLC (0-70% acetonitrile/water) to provide the title compound (19 mg, 57% yield.). MS (apci) m/z = 397.0 (M+H).

WO 2011/006074 PCT/US2010/041538 120 Example 80 F N H 3C O 2. / N ' NH 0 \ (R)-N-tert-butyl-5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1-Yl)yrazolo[1,5 alpyrimidine-3-carboxamide [00554] Prepared by the method described in Example 1 using (R)-5-(2-(5-fluoro-2 methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation K). The combined organic extracts were concentrated and the residue was purified by reverse phase HPLC (0-80% acetonitrile/water) to provide the title compound (23 mg, 68% yield). MS (apci) m/z = 413.0 (M+H). Example 81 F NN

H

3

CON

NH 0 F (R)-5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1-yl)-N-(1-fluoro-2-methylpropan-2 Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide [005551 Prepared by the method described in Example 1 using (R)-5-(2-(5-fluoro-2 methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation K) and 1 -fluoro-2-methylpropan-2-amine. The combined organic extracts were concentrated and the residue was purified by reverse phase HPLC (0-90% acetonitrile/water) to provide the title compound (28 mg, 78% yield). MS (apci) m/z = 431.0 (M+H). Example 82 F N

N

H

3 00O 0 NH2 (R)-5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3 carboxamide WO 2011/006074 PCT/US2010/041538 121 [00556] Prepared by the method described in Example 1 using (R)-5-(2-(5-fluoro-2 methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation K) and 7N NH 3 in MeOH. The combined organic extracts were concentrated and the residue was purified by reverse phase HPLC (0-80% acetonitrile/water) to provide the title compound (15 mg, 38% yield). MS (apci) m/z = 357.0 (M+H). Example 83 F N N NH

H

3 00 0 (R)-5-(2-(5-fluoro-2-methoxvpyridin-3-Vl)pyrrolidin-1-vl)-N-methoxvpyrazolo[1,5 alpyrimidine-3-carboxamide [005571 Prepared by the method described in Example 1 using (R)-5-(2-(5-fluoro-2 methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation K) and O-methylhydroxylamine. The combined organic extracts were concentrated and the residue was purified by reverse phase HPLC (0-80% acetonitrile/water) to yield the title compound (29 mg, 67% yield). MS (apci) m/z = 387.0 (M+H). Example 84 F F ~: N NN N NH 0 CO2H (R)-1-(5-(2-(2,5-difluorophenvll)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3 carboxamido)cyclopropanecarboxylic acid [00558] Step A: Preparation of (R)-ethyl 1-(5-(2-(2,5-difluorophenvll)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxamido)cyclopropanecarboxylate. Using ethyl 1 aminocyclopropanecarboxylate hydrochloride (2.0 equiv) in the procedure described for the synthesis of Example 1, the title compound was obtained as a white solid (61 % yield). MS (apci) m/z = 456.1 (M+H).

WO 2011/006074 PCT/US2010/041538 122 [005591 Step B: Preparation of (R)-1-(5-(2-(2,5-difluorophenyl)yrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxamido)cyclopropanecarboxylic acid. To a solution of the above ester (39 mg, 0.086 mmol) in 2:1 THF-MeOH (1.5 mL) was added IM aq. LiOH (0.257 mL, 257 mmol) and the mixture was stirred at ambient temperature for 18 hours. The mixture was concentrated and the residual solid was dissolved in H 2 0 (3 mL). The solution was treated with IM HCl to pH=3. The resulting precipitate was collected, washed with water and dried in vacuum to yield the title compound as a white solid (31 mg, 83%). MS (apci) m/z = 428.0 (M+H). Example 85 F N NN cN N NH (R)-N-cyclopropyl-5-(2-(3-fluoro-5-(2-morpholinoethoxy)phenyl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00560] Prepared by the method described in Example 76, substituting ammonium chloride with cyclopropylamine in Step F. The crude material was purified by reverse phase HPLC (0-60% acetonitrile/water) to provide the title compound (30 mg, 99% yield.). MS (apci) m/z = 495.1 (M+H). Example 86 F N N

NH

2 0 (R)-5-(2-(5-fluoro-2-(2-morpholinoethoxy) phenyl) pyrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00561] Step A: Preparation of (R)-tert-butyl 2-(2-acetoxy-5 fluorophenvll)pyrrolidine-1-carboxylate. Prepared by the method as described in Preparation A, Step A, substituting 2-bromo-1,4-difluorobenzene with 2-bromo-4-fluorophenyl acetate to afford the title compound (5.75 g, 35% yield). MS (apci) m/z = 224.1 (M+H - Boc). [00562] Step B: Preparation of (R)-4-fluoro-2-(pyrrolidin-2-yl)phenol hydrochloride. Prepared according to the procedure outlined for Example 76, Step B, to afford the title compound (2.64 g, 59.3 % yield). MS (apci) m/z = 182.1 (M+H).

WO 2011/006074 PCT/US2010/041538 123 [005631 Step C: Preparation of (R)-ethyl 5-(2-(3-fluoro-5-hydroxyphenyl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxylate. Prepared by the method as described in Preparation C, Step A, using ethyl 5-chloropyrazolo[1,5-a]pyrimidine-3-carboxylate and (R) 4-fluoro-2-(pyrrolidin-2-yl)phenol hydrochloride. The crude material was purified by reverse phase HPLC (0-65% acetonitrile/water) to provide the title compound (686 mg, 84 % yield). MS (apci) m/z = 371.0 (M+H). [00564] Step D: Preparation of (R)-ethyl 5-(2-(3-fluoro-5-(2-morpholinoethoxy) phenvl)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3-carboxylate. Prepared according to the procedure described in Example 76, Step D, using (R)-ethyl 5-(2-(3-fluoro-5 hydroxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5pyrimidine-3-carboxylate. The crude reaction mixture was purified by reverse phase HPLC (0-60% acetonitrile/water) to provide the title compound (250 mg, 96 % yield). MS (apci) m/z = 484.1 (M+H). [005651 Step E: Preparation of (R)-5-(2-(5-fluoro-2-(2-morpholinoethoxv) phenyl) pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid hydrochloride. To a solution of (R)-ethyl 5-(2-(3-fluoro-5-(2-morpholinoethoxy) phenyl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylate (250 mg, 535 mmol) in MeOH (10 ml) was added IN NaOH (aqueous, 6 mL). The reaction was stirred at ambient temperature for 1 week, then concentrated, treated with 4N HCl in dioxane (5 ml) and concentrated. The crude material was purified by reverse phase HPLC (0-50% acetonitrile/water) to yield the title compound. MS (apci) m/z = 456.1 (M+H). [00566] Step F: Preparation of (R)-5-(2-(5-fluoro-2-(2-morpholinoethoxv) phenyl) pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxamide. Prepared by the method described in Example 76, Step F, using ((R)-5-(2-(5-fluoro-2-(2-morpholinoethoxy) phenyl)pyrrolidin 1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid hydrochloride and ammonium chloride to yield the title compound as a white solid (42.2 mg, 91% yield.). MS (apci) m/z = 455.1

(M+H).

WO 2011/006074 PCT/US2010/041538 124 Example 87 F N N' N N NH (R)-N-cyclopropyl-5-(2-(5-fluoro-2-(2-morpholinoethoxv)phenvl) pyrrolidin-1 yl)yrazolo[1,5-alpyrimidine-3-carboxamide [005671 Prepared by the method described in Example 76, Step F using ((R)-5-(2-(5 fluoro-2-(2-morpholinoethoxy)phenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxylic acid hydrochloride and cyclopropylamine to yield the title compound as a white solid (35.4 mg, 70% yield.). MS (apci) m/z = 495.1 (M+H). Example 88 F FN HN OH OH 5-((R)-2-(2,5-difluorophenyl)pyrrolidin-1-yl)-N-((S)-2,3-dihydroxypropoxy)pyrazolo[1,5 alpyrimidine-3-carboxamide [00568] Step A: Preparation of 5-((R)-2-(2,5-difluorophenvll)pyrrolidin-1-yl)-N-(((S) 2,2-dimethyl-1,3-dioxolan-4-yl)methoxy)pyrazolo[1,5-alpyrimidine-3-carboxamide. To a suspension of (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxylic acid (Preparation C, 100 mg, 0.290 mmol) in CCl 4 (1.5 mL) was added thionyl chloride (0.10 mL, 1.37 mmol) and the mixture was heated at reflux for 2.5 hours. The mixture was cooled to ambient temperature and was concentrated to give a residual brittle foam. The foam was dissolved in dry THF (2.0 mL) and the solution was sequentially treated with DMAP, DIlEA and (S)-O-((2,2-dimethyl-1,3-dioxolan-4-yl)methyl)hydroxylamine (85.5 mg, 0.581 mmol). The reaction was stirred at ambient temperature for 4.5 hours and was concentrated to approx. 0.5 mL. The mixture was diluted with H 2 0 (5 mL) and extracted with 50% EtOAc-hexanes. The combined extracts were washed with IM HCl, H 2 0, IM Na 2

CO

3 and saturated NaCl. The solution was dried over MgSO 4 and activated carbon, then WO 2011/006074 PCT/US2010/041538 125 filtered through a short Si0 2 plug, eluting first with 50% EtOAc-hexanes then 10% MeOH EtOAc. The MeOH-EtOAc pool was concentrated to give a colorless foam. The foam was dissolved in minimal CH 2 Cl 2 and treated with hexanes to give a white suspension. The suspension was concentrated to afford the title compound as white solid that was dried in vacuum (137 mg, 100%). MS (apci) m/z = 474.1 (M+H). [00569] Step B: Preparation of 5-((R)-2-(2,5-difluorophenvl)pyrrolidin-1-yl)-N-((S) 2,3-dihydroxypropoxy)pyrazolo[1,5-aklyrimidine-3-carboxamide To a solution of 5-((R)-2 (2,5-difluorophenyl)pyrrolidin-1-yl)-N-(((S)-2,2-dimethyl-1,3-dioxolan-4-yl)methoxy) pyrazolo[1,5-a]pyrimidine-3-carboxamide (135 mg, 0.285 mmol) in THF (4.0 mL) was added 6M HCl (1.0 mL) dropwise and the mixture was stirred at ambient temperature for 1.5 hours. The reaction mixture was concentrated to approximately 1 mL and was diluted with H 2 0 (5 mL). The resulting milky white mixture was extracted with EtOAc and the combined extracts were washed with IM Na 2

CO

3 and saturated NaCl. The EtOAc solution was dried over MgSO 4 and filtered through a packed Celite pad capped with a layer of MgSO 4 . The solution was concentrated to give a colorless foam that was dissolved in minimal CH 2 Cl 2 and treated with hexanes to give a white suspension. The suspension was concentrated to give the title compound as a white solid that was dried in vacuum (102 mg, 82%). MS (apci) m/z = 434.0 (M+H). Example 89 F §N N

NH

2 0o 0 (R)-5-(2-(5-fluoro-2-(2-methoxvethoxv)phenvll)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3 carboxamide [005701 Step A: Preparation of (R)-methyl 5-(2-(5-fluoro-2-(2-methoxvethoxv)phenvl) pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylate. Prepared by the method described in Example 86, Step D, substituting 4-(2-chloroethyl)morpholine hydrochloride with 1 bromo-2-methoxyethane to afford the title compound (209 mg, 80% yield). MS (apci) m/z = 415.0 (M+H). [005711 Step B: Preparation of (R)-5-(2-(5-fluoro-2-(2-methoxvethoxv)phenvl) pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. Prepared from (R)-methyl 5 (2-(5-fluoro-2-(2-methoxyethoxy)phenyl) pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3- WO 2011/006074 PCT/US2010/041538 126 carboxylate by the method described in Example 77, Step B to afford the title compound (163 mg, 84% yield). MS (apci) m/z = 401.0 (M+H). [00572] Step C: Preparation (R)-5-(2-(5-fluoro-2-(2-methoxyethoxy)phenyl) pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxamide. Prepared by the method described in Example 76, Step F using (R)-5-(2-(5-fluoro-2-(2-methoxyethoxy)phenyl) pyrrolidin-1 yl)pyrazolo[1,5-a] pyrimidine-3-carboxylic acid and ammonium chloride to yield the title compound as a white solid (32.6 mg, 55% yield.). MS (apci) m/z = 400.1 (M+H). Example 90 F 0 / N' (R)-N-cyclopropyl-5-(2-(5-fluoro-2-(2-methoxvethoxv)phenvl) pyrrolidin-1-Vl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00573] Prepared by the method described in Example 89, Step C substituting ammonium chloride with cyclopropylamine to yield the title compound as a white solid (7.9 mg, 12% yield.). MS (apci) m/z = 495.1 (M+H). Example 91 'N F- N (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)-N-(1-methylcyclopropyl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00574] Step A: Preparation of tert-butyl 1 -methylcyclopropylcarbamate. Diphenylphosphoryl azide (2.63 mL, 12.2 mmol) was added to a mixture of 1 methylcyclopropanecarboxylic acid (1.22 g, 12.2 mmol) and TEA (1.70 mL, 12.2 mmol) in anhydrous tert-BuOH (25 mL, 12.2 mmol) under nitrogen, followed by heating first at 50 0 C for 15 minutes, then at 100 0 C for 16 hours. After cooling to ambient temperature, the reaction was concentrated. The crude material was taken up in ether (50 mL), washed with saturated NaHCO 3 and water (50 mL each), and dried (MgSO 4 ), giving the crude product as WO 2011/006074 PCT/US2010/041538 127 white solid (0.81 g, 38% yield), which was used directly in the next step without further purification. [005751 Step B: Preparation of 1-methylcyclopropanamine hydrochloride. A solution of 1-methylcyclopropylcarbamate (250 mg, 1.46 mmol) in HCl (4N dioxane, 3.65 mL, 14.6 mmol) was stirred at ambient temperature for 1 hour. It was then concentrated, triturated with ether, and filtered, giving the product as white solid (78 mg, 50%). [00576] Step C: Preparation of (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)-N-(1 methylcvclopropyl)pyrazolo[1,5-alpyrimidine-3-carboxamide. To a DMF (0.6 mL) solution of (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation I, 10.5 mg, 0.0321 mmol) and HATU (14.6 mg, 0.0385 mmol) was added 1-methylcyclopropanamine hydrochloride (4.14 mg, 0.0385 mmol) and DIEA (0.0168 mL, 0.0962 mmol). After stirring for 10 minutes, the reaction mixture was directly purified by reverse-phase chromatography (5 to 50% acetonitrile/water) to yield the final product as white solid (10 mg, 82%). LCMS (apci) m/z = 381.1 (M+H). Example 92 0 N OH (R)-(5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidin-3-yl)(3-hydroxy-3 methylazetidin- 1 -yl)methanone [005771 Step A: Preparation of 3-methylazetidin-3-ol 2,2,2-trifluoroacetate. To a solution of 1-benzhydryl-3-methylazetidin-3-ol (0.46 g, 1.82 mmol) in EtOH (15 mL) was added TFA (0.14 mL, 1.82 mmol) and Pd(OH) 2 /C (0.127 g, 0.182 mmol). The reaction was subjected to hydrogenation (50 psi) on a Parr shaker at ambient temperature overnight. The reaction mixture was filtered, concentrated and triturated with Et 2 0. The fine white solid was filtered to yield the product as a TFA salt. [00578] Step B: Preparation of (R)-(5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidin-3-yl)(3-hydroxy-3-methylazetidin-1-yl)methanone. To a DMF (1.0 mL) solution of (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid (Preparation I, 85 mg, 0.26 mmol) was added HATU (118 mg, 0.31 mmol) and 3-methylazetidin-3-ol 2,2,2-trifluoroacetate (63 mg, 0.31 mmol) at WO 2011/006074 PCT/US2010/041538 128 ambient temperature, followed by addition of DIEA (0.14 mL, 0.78 mmol) at 0 0 C. After stirring for 5 minutes at ambient temperature, the reaction was directly purified by reverse phase chromatography (5 to 45% acetonitrile/water) to yield the final product as white solid (84 mg, 82%). LCMS (apci) m/z = 397.1 (M+H). Example 93 FN FN HN (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)-N-isopropylpyrazolo[1,5 alpyrimidine-3-carboxamide [005791 To a DMF (1.0 mL) solution of (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation I, 80 mg, 0.244 mmol) was added HATU (112 mg, 0.293 mmol) and propan-2-amine (0.0250 ml, 0.293 mmol) at ambient temperature, followed by drop-wise addition of DIEA (0.128 ml, 0.733 mmol) at 0 0 C. After stirring for 5 minutes at ambient temperature, the reaction was poured into 1:1 water/saturated NaHCO 3 (15 mL) and the layers were separated. The aqueous layer was extracted with EtOAc (3 x 15 mL). The combined organic layers were dried (Na 2

SO

4 ), filtered and concentrated. The crude material was purified by reverse-phase chromatography (5 to 54% acetonitrile/water) to yield the final product as white solid (26 mg, 29%). LCMS (apci) m/z = 369.1 (M+H). Example 94 F N>N N No (R)-(5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidin-3 yl)(pyrrolidin- 1 -yl)methanone [00580] To a solution of (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid (Preparation I, 50 mg, 0.15 mmol) in anhydrous CH 2 Cl 2 (2 mL) was added HOBt (41 mg, 0.31 mmol) followed by EDCI (88 mg, 0.46 mmol). The solution was stirred for 15 minutes, then treated with triethylamine (64 tL, 0.46 mmol) followed by pyrrolidine (38 tL, 0.46 mmol). After stirring at ambient temperature overnight, WO 2011/006074 PCT/US2010/041538 129 the reaction mixture was partitioned between saturated NH 4 Cl (20 mL) and CH 2 Cl 2 (20 mL). The aqueous layer was extracted with CH 2 Cl 2 (2 x 10 mL). The combined organic phases were washed with brine (10 mL), dried over Na 2

SO

4 and concentrated. The crude material was purified by silica chromatography (2 to 5% MeOH/CH 2 Cl 2 ) to yield the final product as white solid (38 mg, 65%). LCMS (apci) m/z = 381.1 (M+H). Example 95

N

N NN HN F F 2 (R)-N-(5-fluoropyridin-2-yl)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00581] To a DMF (0.25 mL) solution of (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation I, 25 mg, 0.076 mmol) and HATU (35 mg, 0.092 mmol) was added 5-fluoropyridin-2-amine (10 mg, 0.092 mmol), followed by drop-wise addition of DIEA (0.040 mL, 0.23 mmol) at ambient temperature. The reaction was heated at 70 0 C overnight, cooled, and directly purified by reverse-phase chromatography (5 to 66% acetonitrile/water) to yield the final product as white solid (25 mg, 78%). LCMS (apci) m/z = 422.0 (M+H). Example 96 F N'~ N 0 [00582] Step A: Preparation of 3-methoxyazetidine 2,2,2-trifluoroacetate. A solution of tert-butyl 3-methoxyazetidine-1-carboxylate (270 mg, 1.44 mmol) in 1:1 TFA/DCM (1 mL) was stirred at ambient temperature for 1 hour and concentrated. The crude product was directly used in the next step assuming quantitative yield. [00583] Step B: Preparation of (R)-(5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidin-3-yl)(3-methoxyazetidin-1-yl)methanone. To a DMF (0.3 mL) solution of (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxylic acid (Preparation I, 30 mg, 0.092 mmol) and HATU (42 mg, 0.11 mmol) was WO 2011/006074 PCT/US2010/041538 130 added 3-methoxyazetidine 2,2,2-trifluoroacetate (22 mg, 0.11 mmol), followed by dropwise addition of DIlEA (0.048 mL, 0.27 mmol). After stirring for 30 minutes at ambient temperature, the reaction was directly purified by reverse-phase chromatography (5 to 50% acetonitrile/water) to yield the final product as white solid (25 mg, 69%). LCMS (apci) m/z = 397.1 (M+H). Example 97 F N CjN HN CI F N-(3-chloro-2-fluoropropyl)-5-((R)-2-(5-fluoropyridin-3-vl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00584] To a DMF (0.3 mL) solution of (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation I, 30 mg, 0.092 mmol) and HATU (42 mg, 0.11 mmol) was added 3-fluoroazetidine hydrochloride (12 mg, 0.11 mmol), followed by DIlEA (0.048 ml, 0.27 mmol) at ambient temperature. After stirring for 2 hours, the reaction was directly purified by reverse-phase chromatography (5 to 58% acetonitrile/water) to yield the product as white solid (8.8 mg, 23%). The isolated product was presumed to result from ring-opening of the azetidine starting material. LCMS (apci) m/z = 421.0 (M+H). Example 98 F N N NO FF (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)-N-(1 (trifluoromethyl)cvclopropyl)pyrazolo[1,5-alpyrimidine-3-carboxamide [005851 Step A: Preparation of tert-butyl 1-(trifluoromethyl)cvclopropylcarbamate. Diphenylphosphoryl azide (0.462 mL, 2.14 mmol) was added drop-wise to a stirred mixture of 1-(trifluoromethyl)cyclopropanecarboxylic acid (300 mg, 1.95 mmol), TEA (0.271 mL, 1.95 mmol) and 4A molecular sieves in anhydrous tert-BuOH (4 mL) under nitrogen at ambient temperature. The reaction was heated to reflux for 18 hours, then cooled, filtered, WO 2011/006074 PCT/US2010/041538 131 and concentrated, and the residue was taken up in ether (20 mL). The organic layer was washed with saturated NaHCO 3 and water (20 mL each), dried (Na 2

SO

4 ), filtered and concentrated, giving the crude product as white solid (0.32 g, 72%). The crude product was used directly in the next step without further purification. [00586] Step B: Preparation of 1-(trifluoromethyl)cyclopropanamine hydrochloride. A solution of tert-butyl 1-(trifluoromethyl)cyclopropylcarbamate (0.3 g, 1.3 mmol) in HCl (4 N dioxane, 6.7 mL, 27 mmol) was stirred at ambient temperature overnight. The reaction was then concentrated to yield the crude product as white solid, which was used directly in the next step assuming quantitative yield. [005871 Step C: Preparation of (R)-5-(2-(5-fluoropyridin-3-vl)pyrrolidin-1-yl)-N-(1 (trifluoromethyl)cyclopropyl)yrazolo[1,5-alpyrimidine-3-carboxamide. To a DMF (0.4 mL) solution of (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxylic acid (Preparation I, 50 mg, 0.15 mmol) and HATU (87 mg, 0.23 mmol) was charged 1-(trifluoromethyl)cyclopropanamine hydrochloride (37 mg, 0.23 mmol), followed by drop-wise addition of DIEA (0.080 mL, 0.46 mmol). After stirring first at ambient temperature for 15 minutes and then at 85 0 C overnight, the reaction was cooled and directly purified by reverse-phase chromatography (5 to 60% acetonitrile/water) to yield the final product as off-white solid (15 mg, 23%). LCMS (apci) m/z = 435.0 (M+H). [00588] The compounds listed in Table A were prepared according to the method described in Example 91, 92, 93 or 94, by reacting (R)-5-(2-(5-fluoropyridin-3-yl)pyrrolidin 1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation I) with appropriate amine starting materials in the presence of an amide coupling reagent (e.g. HATU, EDCI/HOBt) and an organic base (e.g. DIEA, TEA) in an appropriate solvent (e.g. DMF, DCM). Table A Ex. # Structure Chemical Name Data FN 5-((R)-2-(5-fluoropyridin-3- LCMS (apci) 99 HN 0 yl)pyrrolidin- 1 -yl)-N-((trans)-4- z425.1 hydroxycyclohexyl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide

OH

WO 2011/006074 PCT/US2010/041538 132 Ex. # Structure Chemical Name Data 5-((R)-2-(5-fluoropyridin-3- LCMS (apci) 100 N yl)pyrrolidin-1-yl)-N-((cis)-4- z= 425.1 HNhydroxycyclohexyl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide OH _______ F' (R)-N-cyclobutyl-5-(2-(5 101N fluoropyridin-3-yl)pyrrolidin-1- LCMS (apci) 0HN yl)pyrazolo[1,5-a]pyrimidine-3- mz H381.1 carboxamide (M+H) F N (R)-5-(2-(5-fluoropyridin-3 -CMS (apci) 102 N N yl)pyrrolidin- 1 -yl)-N-(1- = 395.1 12NH O methylcyclobutyl)pyrazolo[ 1,5- (M+H) a]pyrimidine-3-carboxamide 5-((R)-2-(5-fluoropyridin-3- LCMS (apci) 103 yl)pyrrolidin- 1 -yl)-N-((1 S,2S)-2- m Sz = 411.1 HN O hydroxycyclopentyl)pyrazolo[1,5- (M+H) < OH a]pyrimidine-3-carboxamide N-N 5-((R)-2-(5-fluoropyridin-3 104 N yl)pyrrolidin- 1 -yl)-N-((1 S,2R)-2- m Sz = 411.1 HN O hydroxycyclopentyl)pyrazolo[ 1,5 OH a]pyrimidine-3-carboxamide (M+H) 5-((R)-2-(5-fluoropyridin-3- CMS (apci) yl)pyrrolidin-1-yl)-N-((1S,3S)-3- = 411.1 H hydroxycyclopentyl)pyrazolo[1,5- (M+H) -OH a]pyrimidine-3-carboxamide F-N\(R)-N-(cyclopropylmethyl)-5-(2-(5- LCMS (apci) 106 N 'N- fluoropyridin-3-yl)pyrrolidin-1 m/Z = 381.1 H yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) carboxamide (R)-5-(2-(5-fluoropyridin-3- LCMS (apci) 107 N N yl)pyTTolidin- 1 -yl)-N-(1- m/z = 397.1 HN O (hydroxymethyl)cyclopropyl)pyrazolo (M+H) [1,5-a]pyrimidine-3-carboxamide

OH

WO 2011/006074 PCT/US2010/041538 133 Ex. # Structure Chemical Name Data F N- (R)-(5-(2-(5-fluoropyridin-3- CMS (apci) 1 yl)pyrrolidin-1-yl)pyrazolo[1,5- m = 383. 108 N a]pyrimidin-3-yl)(3-hydroxyazetidin- mz= 383.1 1 -yl)methanone (M+H) F N 5-((R)-2-(5-fluoropyridin-3- CMS (apci) 109N yl)pyTTolidin- 1 -yl)-N-((S)-2- m/z = 385.1 109HN O hydroxypropyl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide HO F N 5-((R)-2-(5-fluoropyridin-3- CMS (apci) 110 /-N N yl)pyrrolidin- 1 -yl)-N-((R)-2- m/z = 385.1 HN O hydroxypropyl)pyrazolo[1,5- (M+H) y- a]pyrimidine-3-carboxamide HO (R)-5-(2-(5-fluoropyridin-3 111 N yl)pyrrolidin-I-yl)-N-(2-hydroxy-2- MS 399.1 HNO methylpropyl)pyrazolo[1,5- (M+H)39. HNJ~ a]pyrimidine-3-carboxamide (M+H) OH _______ F N (R)-5-(2-(5-fluoropyridin-3 -CMS (apci) 112 N N ' yl)pyrrolidin-1-yl)-N-(2- m/z = 371.1 112H~ N O hydroxyethyl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide OH F _0 N-N N-(1-cyclopropylethyl)-5-((R)-2-(5 - CMS (apci) 113 N N fluoropyridin-3-yl)pyrrolidin-1- m/z = 395.1 H yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) carboxamide F N (R)-5-(2-(5-fluoropyridin-3 -CMS (apci) yl)pyrrolidin- 1 -yl)-N- 341.1 114 /-N V methylpyrazolo [1,5 -a]pyrimidine-3 - (M+H) HN carboxamide F N-N 5-((R)-2-(5-fluoropyridin-3 -CMS (apci) 11C Ns yl)pyrrolidin-1I-yl)-N-((R)-1I- Lmz =385.1 115 1- 1,5- mlz = 385.1 N_H hydroxypropan-2-yl)pyrazolo[1,5- (M+H) OH a]pyrimidine-3-carboxamideII WO 2011/006074 PCT/US2010/041538 134 Ex. # Structure Chemical Name Data F N-N 5-((R)-2-(5-fluoropyridin-3- LCMS (apci) 116 a s , yl)pyrrolidin- 1 -yl)-N-((S)-1- mz= 385.1 HN O hydroxypropan-2-yl)pyrazolo[1,5- (M+H) -OH a]pyrimidine-3-carboxamide -N (R)-5-(2-(5-fluoropyridin-3- LCMS (apci) 117 yl)pyrrolidin-1-yl)pyrazolo[1,5- m/z = 327.0 o a]pyrimidine-3-carboxamide (M+H)

H

2 N_ F C5 :,N'N r 5-((R)-2-(5-fluoropyridin-3- CMS aci) N yl)pyrrolidin-1-yl)-N-(1- m = 399. HN methoxypropan-2-yl)pyrazolo[1,5- mz = 399.1 a]pyrimidine-3-carboxamide (M+H) /0 N ~N'N F N N 5-((R)-2-(5-fluoropyridin-3- LCMS (apci) 119 H O yl)pyrrolidin-1-yl)-N-(2-hydroxy-3- m/z = 415.1 HOO methoxypropyl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide 0 5-((R)-2-(5-fluoropyridin-3- LCMS (apci) 120 yl)pyrrolidin- 1 -yl)-N-((trans)-2- m/z = 411.1 HN O hydroxycyclopentyl)pyrazolo[1,5- (M+H) OH a]pyrimidine-3-carboxamide F N 5-((R)-2-(5-fluoropyridin-3- LCMS (apci) 121 N 5 O yl)pyrrolidin-1-yl)-N-((S)-1-hydroxy- m/z = 413.1 H O 3-methylbutan-2-yl)pyrazolo[1,5- (M+H) SOH a]pyrimidine-3-carboxamide F N''N 5-((R)-2-(5-fluoropyridin-3- LCMS (apci) 122 O yl)pyrrolidin- 1 -yl)-N-((R)- 1 -hydroxy- m/z = 413.1 HN OH 3-methylbutan-2-yl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide F N- N-((R)-1-cyclopropylethyl)-5-((R)-2- LCMS (apci) 123 N (5-fluoropyridin-3-yl)pyrrolidin-1- m/z = 395.1 yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) carboxamide WO 2011/006074 PCT/US2010/041538 135 Ex. # Structure Chemical Name Data F

-

N-((S)-1-cyclopropylethyl)-5-((R)-2- LCMS (apci) 124 N1 N (5-fluoropyridin-3-yl)pyrrolidin-1- m/z = 395.1 124HNN~O yl)pyrazolo[1,5-a]pyrimidine-3- M+H) carboxamide (M+H) F- 0N-N (R)-5-(2-(5-fluoropyridin-3 -CMS (apci) 125 N yl)pyrrolidin-1-yl)-N-(3-hydroxy-2,2- mz= 413.1 HN dimethylpropyl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide F (R)-azetidin-1-yl(5-(2-(5- LCMS (apci) 126 NN fluoropyridin-3-yl)pyrrolidin-1- = 367.1 02 yl)pyrazolo[1,5-a]pyrimidin-3- (M+H) Q yl)methanone (M+H) F N -N (R)-(5-(2-(5-fluoropyridin-3 yl)pyrrolidin-1-yl)pyrazolo[1,5- LCMS (apci) 127 0 a]pyrimidin-3-yl)(3- m/z = 397.1 N (hydroxymethyl)azetidin- 1- (M+H) yl)methanone OH _______ (5-((R)-2-(5-fluoropyridin-3- CMS (apci) 128 N yl)pyrrolidin-1-yl)pyrazolo[1,5- m/z = 397.1 N O a]pyrimidin-3-yl)((S)-3- (M+H) hydroxypyrrolidin- 1 -yl)methanone HO _______ N 0 5-((R)-2-(5-fluoropyridin-3 -CMS (apci) 129 N yl)pyrrolidin- 1 -yl)-N-((R)- 1,1,1- m= 423.0 12H O trifluoropropan-2-yl)pyrazolo[1,5- (M+H) F .,,,, a]pyrimidine-3-carboxamide F F N' 5-((R)-2-(5-fluoropyridin-3- CMS (apci) N yl)pyrrolidin- 1 -yl)-N-((S)- 1,1,1 - m = 423.0 130~H O trifluOOpOpan-2-yl)pyraZOlO[1,5- (M+H)43. F Ha]pyrimidine-3-carboxamide F F____ WO 2011/006074 PCT/US2010/041538 136 Ex. # Structure Chemical Name Data N(R)-5 -(2-(5 -fluoropyridin-3 -LCS(p) 131 yl)pyrrolidin-1I-yl)-N-(2,2,2- m1z=409.0 N trifluoroethyl)pyrazolo[ 1,5- (M+H) 'NY F a]pyrimidine-3-carboxamide r-NN(R)-5 -(2-(5 -fluoropyridin-3 -LCS(p) yl)pyrrolidin- 1 -yl)-N-( 1 -hydroxy-2- CS(pi 132N meypropan--yIpyrazooL15 (M+H)39. HN OH a]pyrimidine-3-carboxamide MH CN5-((R)-2-(5-fluoropyridin-3- LCMS (apci) 133 NNyl)pyrrolidin- 1 -yl)-N-(( 1 R,2R)-2- mz= 411.1 hydroxycyclopentyl)pyrazolo [1,5 - (M+H) .,OH a]pyrimidine-3-carboxamide N-N (R)-N-(2,2-difluoroethyl)-5-(2-(5 14fluoropynidin-3 -yl)pyrrolidin- 1- LMS (a391.0 134 N yI)pyrazolo[1,5-a]pyrimidine-3-ml 39. KN F carboxamide (M+H) C'N5-((R)-2-(5-fluoropyridin-3- LCMS (apci) 135 NNyl)pyrrolidin- 1 -yl)-N-(( 1 R,2S)-2- mz= 411.1 hydroxycyclopentyl)pyrazolo[ 1,5 - (M+H) j.OH a]pyrimidine-3-carboxamide N5-((R)-2-(5-fluoropyridin-3-LCS(p) 136 N ~yl)pyrrolidin- 1 -yl)-N-(( 1 R,2R)-2- /=42. 6 -1H a]pyrimidine-3-carboxamide(MH N (R)-(5 -(2-(5 -fluoropyridin-3 -LCS(p) 137 ~yl)pyrrolidin-1-yl)pyrazolo[1,5- 39. 137N a]pyrimidin-3-yl)(piperidin- 1- (M+H)35. j J yl)methanone 5-((R)-2-(5-fluoropyridin-3 J~N yl)pyrrolidin- 1-yl)-N-((2R,3 S,4S)-3 - LCMS (apci) 138 j (hydroxymethyl)bicyclo[2.2. 1]heptan- mlz = 451.2 02-yl)pyrazolo[1 ,5-a]pyrimidine-3- (M+H) H carboxamide _________ ~OH___ _____ WO 2011/006074 PCT/US2010/041538 137 Example 139 N O N~ 'N N HO (R)-(5-(2-(5-fluoro-2-methoxvpyridin-3-vl)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidin 3 -yl)(3 -hydroxyazetidin- 1 -yl)methanone [005891 To a DMF (0.4 mL) solution of (R)-5-(2-(5-fluoro-2-methoxypyridin-3 yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation K, 30 mg, 0.084 mmol) was added HATU (38 mg, 0.10 mmol) and azetidin-3-ol hydrochloride (11 mg, 0.10 mmol) at ambient temperature, followed by dropwise addition of DIEA (0.044 ml, 0.25 mmol) at 0 0 C. After stirring for 20 minutes at ambient temperature, the reaction was directly purified by reverse-phase chromatography (5 to 50% acetonitrile/water) to yield the final product as white solid (26 mg, 75%). LCMS (apci) m/z = 413.1 (M+H). Example 140 F

ONN

b SNH OH 5-((R)-2-(5-fluoro-2-methoxvpyridin-3-Vl)pyrrolidin-1-vl)-N-((trans)-4 hydroxycyclohexyl)pyrazolo[1,5-alpyrimidine-3-carboxamide [005901 To a solution of (R)-5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation K, 30 mg, 0.084 mmol) in anhydrous CH 2 Cl 2 (2 mL) was added HOBt (34 mg, 0.25 mmol) followed by EDCI (48 mg, 0.25 mmol). The solution was stirred for 30 minutes, then treated with (trans)-4 aminocyclohexanol (29 mg, 0.25 mmol) followed by triethylamine (35 tL, 0.25 mmol). After stirring at ambient temperature for 5 hours, the reaction mixture was diluted with EtOAc, washed with saturated NH 4 Cl (20 mL), saturated NaHCO 3 , and brine, then dried (Na 2

SO

4 ), filtered and concentrated. The crude material was purified by silica chromatography (4% MeOH/CH 2 Cl 2 ) to yield the final product as white solid (23 mg, 60%). LCMS (apci) m/z = 455.1 (M+H).

WO 2011/006074 PCT/US2010/041538 138 Example 141 F N H -o N0 +0 (R)-tert-butyl 3-(5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamido)propylcarbamate [005911 To a mixture of (R)-5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation K, 200 mg, 0.560 mmol) and HATU (255 mg, 0.672 mmol) in DMF (2 mL) was added DIlEA (292 gL, 1.68 mmol), followed by dropwise addition of tert-butyl 3-aminopropylcarbamate (117 mg, 0.672 mmol) at ambient temperature. After stirring for 3 hours, the reaction was directly purified by reverse-phase chromatography (5 to 70% acetonitrile/water) to yield the final product as white solid (250 mg, 87%). LCMS (apci) m/z = 414.1 (M+H-Boc). Example 142 F , HN F0 HN HN O N \ ,N H2 (R)-N-(3-aminopropyl)-5-(2-(5-fluoro-2-oxo-1,2-dihydropyridin-3-yl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00592] A mixture of (R)-tert-butyl 3-(5-(2-(5-fluoro-2-methoxypyridin-3 yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamido)propylcarbamate (Example 141, 70 mg, 0.14 mmol) and HCl (4 N dioxane, 1.7 mL, 6.8 mmol) in a pressure reaction tube was heated at 85 0 C for 12 hours then concentrated under reduced pressure. The crude material was purified by reverse-phase chromatography (5 to 40% acetonitrile/water) to yield the final product as white solid. LCMS (apci) m/z = 400.1 (M+H). Example 143 F NN H SOH 0 H OH WO 2011/006074 PCT/US2010/041538 139 N-((S)-2,3-dihydroxvpropyl)-5-((R)-2-(5-fluoro-2-methoxvpyridin-3-vl)pyrrolidin- 1 yl)yrazolo[ 1,5-alpyrimidine-3-carboxamide [00593] Step A: Preparation of N-(((S)-2,2-dimethyl-1,3-dioxolan-4-Yl)methyl)-5 ((R)-2-(5-fluoro-2-methoxvpyridin-3-vl)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3 carboxamide. Prepared according to the method described in Example 140, replacing (trans) 4-aminocyclohexanol with (S)-(2,2-dimethyl-1,3-dioxolan-4-yl)methanamine. LCMS (apci) m/z = 471.0 (M+H-Boc). [00594] Step B: Preparation of N-((S)-2,3-dihydroxvpropyl)-5-((R)-2-(5-fluoro-2 methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]lyrimidine-3-carboxamide. To a solution of N-(((S)-2,2-dimethyl-1,3-dioxolan-4-yl)methyl)-5-((R)-2-(5-fluoro-2 methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide (36 mg, 0.077 mmol) in THF (2 mL) was added HCl (3 N aq.) at ambient temperature. The resulting mixture was stirred for 5 hours. The reaction was diluted with EtOAc, washed with saturated

NH

4 Cl and brine, then dried (MgSO 4 ), filtered and concentrated. The crude material was rinsed with ether to yield the final product as white solid (30 mg, 91%). LCMS (apci) m/z = 431.1 (M+H). Example 144 N O0O HN -O ci N-((S)-3-chloro-2-hydroxypropyl)-5-((R)-2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide [005951 Step A: Preparation of (S)-1-amino-3-chloropropan-2-ol hydrochloride. To a solution of benzaldehyde (4.50 g, 42.4 mmol) in EtOH (12 mL) was added aqueous ammonia (4.01 g, 65.9 mmol) in several portions. After stirring for 10 minutes, (S)-2 (chloromethyl)oxirane (3.81 g, 41.2 mmol) was added and the reaction mixture was stirred for 2 hours at ambient temperature. The reaction mixture was then heated at 35-40 0 C with a heating mantle for 6 hours, followed by stirring at ambient temperature for 18 hours. The reaction was concentrated to 5 mL and toluene (5 mL) was added. The mixture was heated to 36 0 C and a solution of concentrated HCl (6.09 g, 61.8 mmol) and water (5.9 mL) was added slowly over 5 minutes to maintain an internal reaction temperature range of 36-41 0 C. The biphasic mixture was heated at 42-45 0 C for 3 hours. The organic phase was separated and WO 2011/006074 PCT/US2010/041538 140 washed with water (10 mL). The aqueous phases were combined and ethanol (10 mL) was added. The mixture was concentrated to 10 mL, and ethanol (6 x 10 mL) was added, concentrating after each addition. After the last concentration step, the slurry was warmed to reflux, cooled to ambient temperature, and then placed at -20 0 C for 18 hours. The product was collected by vacuum filtration, washed with cold ethanol, and vacuum-dried, to provide the product as white solid (3.58 g, 60% yield). [005961 Step B: Preparation of N-((S)-3-chloro-2-hydroxypropyl)-5-((R)-2-(5-fluoro 2-methoxvpyridin-3-vl)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide. Prepared by the method described in Example 139, replacing azetidin-3-ol hydrochloride with (S)-1 amino-3-chloropropan-2-ol hydrochloride. LCMS (apci) m/z = 449.0 (M+H) Example 145 F NN

-

OH ---O N0N C N-((R)-3-chloro-2-hydroxvpropyl)-5-((R)-2-(5-fluoro-2-methoxvpyridin-3-vl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [005971 Step A: Preparation of (R)-1-amino-3-chloropropan-2-ol hydrochloride. Prepared by the method described in Example 144, Step A, replacing (S)-2 (chloromethyl)oxirane with (R)-2-(chloromethyl)oxirane. [00598] Step B: Preparation of N-((R)-3-chloro-2-hydroxvpropyl)-5-((R)-2-(5-fluoro 2-methoxypyridin-3-yl)lpyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxamide. Prepared by the method described in Example 139, replacing azetidin-3-ol hydrochloride with (R)-1 amino-3-chloropropan-2-ol hydrochloride. LCMS (apci) m/z = 449.0 (M+H) Example 146 F O N~ §N P _ HN --- O N HNO C (R)-N-(2-chloroethoxy)-5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [005991 Step A: Preparation of 2-(2-chloroethoxy)isoindoline-1,3-dione. A 1 L round-bottomed flask was charged 2-hydroxyisoindoline-1,3-dione (16.6 g, 98.71 mmol), followed by DMF (100 mL), then 1-bromo-2-chloroethane (25.2 mL, 296.1 mmol), and then WO 2011/006074 PCT/US2010/041538 141 triethylamine (42.1 mL, 296.1 mmol). After stirring at ambient temperature overnight, the reaction mixture was filtered (GF/F) and rinsed with DMF. The filtrate (250 mL) was poured into ice-water (2 L) while stirring, and the resulting precipitate was filtered, rinsed with water, and dried, yielding the crude product as a white solid (21 g). The crude product was triturated with heptane (3 x 400 mL), filtered and air-dried, giving the product as white solid (17.6 g, 79%). [00600] Step B: Preparation of O-(2-chloroethyl)hydroxylamine hydrochloride. A 1 L three-necked round-bottomed flask was charged HCl (6 M aq., 295 mL, 1773 mmol), followed by 2-(2-chloroethoxy)isoindoline-1,3-dione (10 g, 44.3 mmol) with stirring. A water condenser was attached and the reaction was refluxed at 100 0 C for 2 hours, then stirred at ambient temperature overnight. The reaction mixture was filtered. Absolute EtOH was added to the filtrate and filtrate was concentrated. The crude material was triturated from hot EtOH to yield the first crop of product as white solid (2.2 g). The mother liquor was concentrated and triturated as described, yielding a second crop of product (1.7) g. [00601] Step C: Preparation of (R)-N-(2-chloroethoxy)-5-(2-(5-fluoro-2 methoxypyridin-3-vl)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide. Prepared by the method described in Example 139, replacing azetidin-3-ol hydrochloride with 0-(2 chloroethyl)hydroxylamine hydrochloride. LCMS (apci) m/z = 434.9 (M+H) [00602] The compounds listed in Table B were prepared according to the method described in Example 139 or Example 140, by reacting (R)-5-(2-(5-fluoro-2-methoxypyridin 3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation K) with an appropriate amine starting material in the presence of an amide coupling reagent (e.g. HATU, EDCI/HOBt) and an organic base (e.g. DIEA, TEA) in an appropriate solvent (e.g. DMF, DCM). Table B Ex. # Structure Chemical Name Data ,N (R)-(5-(2-(5-fluoro-2 / 0 methoxypyridin-3-yl)pyrrolidin-1- LCMS (apci) 147 N N yl)pyrazolo[1,5-a]pyrimidin-3- m/z = 427.1 - yl)(3-hydroxy-3-methylazetidin-1- (M+H) 0 HO O yl)methanone

I

WO 2011/006074 PCT/US2010/041538 142 Ex. # Structure Chemical Name Data N' N- (R)-5-(2-(5-fluoro-2 Fmethoxypyridin-3 -yl)pyrrolidin- 1- LCMS (apci) 148 02N~ yl)-N-(3- m/z = 415.1 hydroxypropyl)pyrazolo[ 1,5- (M+H) ,'OH a]pyrimidine-3 -carboxamide F N N'Q$ NP - N-(2,3-dihydroxypropyl)-5-((R)-2- LCS(p) 149 NHN (5-fluoro-2-methoxypyridin-3- mz= 43 1.1 -0 yl)pyrrolidin- 1 -yl)pyrazolo [ 1,5 - MH HO a]pyrimidine-3-carboxamide(MH

N

1 0 P NNN-((R)-2,3 -dihydroxypropyl)-5 - LCS(c) 150 0N~ 1 (()2(-loo2mehxprdn = 43 1.0 OH a]pyrimidine-3-carboxamide(MH N / 0 N-N(R)-5-(2-(5-fluoro-2 N methoxypyridin-3-yl)pyrrolidin-1- LCMS (apci) 151 K7 HN 0 yl)-N-(4- m/z = 429.1 hydroxybutyl)pyrazolo[ 1,5- (M+H) a]pyrimidine-3 -carboxamide OH ___________________ _________ N O 0(R)-N-(2-tert-butoxyethoxy)-5 -(2 15 N~ L o (5-fluoro-2-methoxypyridin-3- LCMS (apci) 0 yl)pyrrolidin- 1-yl)pyrazolo [1,5 - (M+H) I alpyrimidine-3-carboxamide F N (R)-5-(2-(5-fluoro-2 153 )~'N methoxypyridin-3-yl)pyrrolidin-1- MS (apci) m/z= 15 yl)-N-methylpyrazolo[1,5- 371.1 (M+H) N j N a]pyrimidine-3-carboxamide O H __ _ _ _ _ _ _ _ _ _ _ _ _ F NN5 -((R)-2-(5 -fluoro-2 0methoxypyridin-3-yl)pyrrolidin-1- MS(p)m/= 154 ~ N I NH yl)-N-((1S,3S)-3- 1,- 44 1.1 (M+H) (OH a]pyrimidine-3-carboxamide________ F N 01-/(R)-5-(2-(5-fluoro-2 --0 ~~~methoxypyridin-3-yl)pyrrolidin-l 1 S(pi / 155 ~ ~N\yl)-N-(2- 401.1 (M+H) 0 NkH hydroxyethyl)pyrazolo[1,5 ( a]pyrimidine-3 -carboxamide WO 2011/006074 PCT/US2010/041538 143 Ex. # Structure Chemical Name Data F 5 -((R)-2-(5 -fluoro-2 15 methoxypyridin-3-yl)pyrrolidin-1- MS (apci) m/z 0NQKN hydroxypropyl)pyrazolo [1,5 -41.(MH OH a]pyrimidine-3-carboxamide________ N F 5 -((R)-2-(5 -fluoro-2 157 - 0%11 r N methoxypyridin-3-yl)pyrrolidin-1- MS(p)m/ 157 N L NH yl)-N-((R)-2-41. MH 0 hydroxypropyl)pyrazolo [1,5 -41.(MH OH a]pyrimidine-3-carboxamide________ F (R)-5-(2-(5-fluoro-2 r"NNmethoxypyridin-3 -yl)pyrrolidin- 1- MS(p)m/ 158 yl)-N-(2-hydroxy-2 o methylpropyl)pyrazolo [1,5-42.(MH OH a]pyrimidine-3-carboxamide________ F (R)-5-(2-(5-fluoro-2 0 ~metho xypyri din- 3-yl)pyrroIi din- 1 159 CYr- NH~ yl)-N-(1 -(2- MS (apci) m/z O hydroxyethyl)piperidin-4- 484.2 (M+H) N yl)pyrazolo[1 ,5-a]pyrimidine-3 V-- carboxamide OH _____________________ __________ F N (R)-N-( 1,3 -dihydroxypropan-2-yl) 160 .- 5-(2-(5-fluoro-2-methoxypyridin-3- MS (apci) m/z < H yl)pyrrolidin- 1-yl)pyrazolo [1,5 - 43 1.1 (M+H) 0 a]pyrimidine-3 -carboxamide N -N (R)-5-(2-(5-fluoro-2 0 methoxypyridin-3-yl)pyrrolidin- 1 MS(p- / 161 C N NH yl)-N-(6-oxo- 1,6-dihydropyridin-3- 450. (M+Hi) / 0 ~yl)pyrazolo[1 ,5-a]pyrimidine-3- 45. MH HN carboxamide 0F____________________ _________ _N (R)-5-(2-(5-fluoro-2 j~~~ ~~ methoxypyridin-3-yl)pyrrolidin-1 MS (ac) / 162 N I O yl)-N-(1 -(methylsulfonyl)piperidin-MS(pi / 0 N 4-yl)pyrazolo [1,5 -a]pyrimidine-3 - 5 18.1 (M+H) _Nr carboxamide NF P (R)-N-(2-chloroethyl)-5 -(2-(5 - LCMS (apci) 163 §-N HNf fluoro-2-methoxypyridin-3- =1. N yl)pyrrolidin- 1 -yl)pyrazolo [1,5 -0 \C-\ CI a]pyrimidine-3-carboxamide (M+H) WO 2011/006074 PCT/US2010/041538 144 Ex. # Structure Chemical Name Data N (R)-N-(2-bromoethoxy)-5-(2-(5- LCMS (apci) 164 N N 0O fluoro-2-methoxypyridin-3- = 479.0 -O HN, 10 -Br yl)pyrrolidin-1-yl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide [00603] The compounds listed Table C were prepared by the method described in Examples 1, 139 or 140, by reacting (R)-5-(2-(2,5-difluorophenyl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation C) with an appropriate amine starting material in the presence of an amide coupling reagent (e.g. HATU, EDCI/HOBt) and an organic base (e.g. DIEA, TEA) in an appropriate solvent (e.g. DMF, DCM). Table C Ex. # Structure Chemical Name Data F 5-(2-(2,5-difluorophenyl)pyrrolidin- LCMS (apci) 165 F 1-yl)-N-(2- m/z = 388.1 N H hydroxyethyl)pyrazolo[1,5- (M+H) O a]pyrimidine-3-carboxamide OH 5-((R)-2-(2,5- LCMS (apci) 166 F N' difluorophenyl)pyrrolidin- 1 -yl)-N-(2- 402.1 O NH hydroxypropyl)pyrazolo[1,5- (M+H) o ' a]pyrimidine-3-carboxamide OH F F 5-((R)-2-(2,5- LCMS (apci) 167 F N difluorophenyl)pyrrolidin- 1 -yl)-N-(2- z= 402.1 ONH hydroxypropyl)pyrazolo[1,5- (M+H) o 9a]pyrimidine-3-carboxamide F 5-((R)-2-(2,5 N difluorophenyl)pyrrolidin-1I-yl)-N-(3 - LCMS (apci) 168 F hydroxy-2,2- m/z = 430.2 N dimethylpropyl)pyrazolo[1,5- (M+H) OH a]pyrimidine-3-carboxamide F 5-((R)-2-(2,5 F N difluorophenyl)pyrrolidin- 1 -yl)-N- LCMS (apci) 169 N ((IS, 3S)-3- m/z = 428.1 O NH hydroxycyclopentyl)pyrazolo[1,5- (M+H) O_0H I a]pyrimidine-3-carboxamide I _I WO 2011/006074 PCT/US2010/041538 145 Ex. # Structure Chemical Name Data N difluorophenyl)pyrrolidin- 1-yl)-N-(2- LCMS (apci) 170 0NIH(4-hydroxypiperidin-1I- m/z = 471.2 N yl)ethyl)pyrazolo[ 1,5-a]pyrimidine-3- (M+H) carboxamide /~~ ~difluorophenyl)pyrrolidin- 1 -yl)-N-(2- LCMS (apci) 171 N N (4-methylpiperazin- 1 - m/z = 470.2 N yl)ethyl)pyrazolo [1,5 -a]pyrimidine-3 - (M+H) Q N carboxamide 5-((R)-2-(2,5- LCMS (apci) 172 difluorophenyl)pyrrolidin- 1 -yl)-N-(2- mz= 402.1 NI NH methoxyethyl)pyrazolo[ 1,5- MH 0 a]pyrimidine-3-carboxamide(MH F N difluoronhenvl~pvrrolidin- 1 -vh)-N- LCMS (anci) 173 Ny - N (1,3-dihydroxypropan-2- mlz = 418.1 o V-\ yl)pyrazolo [1,5 -a]pyrimidine-3 - (M+H) < OH carboxamide F' P [N\- difluorophenyl)pyrrolidin-1I-yl)-N- LCMS (apci) 174 (JN ' NNO ((2S,3R)- 1,3-dihydroxybutan-2- mlz = 432.1 O yl)pyrazolo [1,5 -a]pyrimidine-3 - (M+H) carboxamide F N difluorophenyl)pyrrolidin- 1 -yl)-N- LCMS (apci) 175 CN - N OH ((2S,3S)-1,3-dihydroxybutan-2- mlz = 432.1 O yl)pyrazolo [1,5 -a]pyrimidine-3 - (M+H) carboxamide F' P [N\- difluorophenyl)pyrrolidin-1I-yl)-N- LCMS (apci) 176 (JN "*N N OH ((2R,3S)-1,3-dihydroxybutan-2- mlz = 432.1 O yl)pyrazolo [ 1,5 -a]pyrimidine-3 - (M+H) carboxamide Fadifluorophenyl)pyrrolidin- 1 -yl)-N- LCMS (apci) 177 CNC ((S)-1I-hydroxypropan-2- mlz = 402.1 N NH OH yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) O ~carboxamide________ WO 2011/006074 PCT/US2010/041538 146 Ex. # Structure Chemical Name Data F 5-((R)-2-(2,5 F1 N-N difluorophenyl)pyrrolidin- 1 -yl)-N- LCMS (apci) 178 CN N *-NHO ((S)-1I-hydroxybutan-2- mlz = 416.1 O yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) carboxamide F 5-((R)-2-(2,5 F -N difluorophenyl)pyrrolidin- 1 -yl)-N- LCMS (apci) 179 N ((S)-1-hydroxy-3-methylbutan-2- m/z = 430.1 O yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) carboxamide F 5-((R)-2-(2,5 F'C r difluorophenyl)pyrrolidin-1I-yl)-N- LCMS (apci) 180 N ((S)--hydroxy-3,3-dimethylbutan-2- m Sz = 444.2 ONHN yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) carboxamide Example 181 F N~ NNH N-cyclopropyl-5-(2-(5-fluoro-2-methylpyridin-3-vl)pyrrolidin-1-Vl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00604] To a solution of (R)-5-(2-(5-fluoro-2-methylpyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation 0, 50 mg, 0.15 mmol) in DCM (2 mL) was added HOBt (40 mg, 0.29 mmol) followed by EDCI (84 mg, 0.44 mmol). The solution was stirred at ambient temperature for 15 minutes, then treated with triethylamine (61 gL, 0.44 mmol) followed by cyclopropylamine (31 gL, 0.44 mmol). After stirring for 16 hours the mixture was partitioned between saturated NH 4 Cl solution (20 mL) and DCM (20 mL) and the aqueous layer was extracted with DCM (2 x 10 mL). The combined organic phases were washed with brine (10 mL), dried over Na 2

SO

4 , filtered and concentrated. The residue was purified by silica gel column chromatography, eluting with 2-4% MeOH/DCM, to afford the title product as white solid (44 mg, 79%). MS (apci) m/z = 381.1 (M+H).

WO 2011/006074 PCT/US2010/041538 147 Example 182 F N NH N-cyclopropyl-5-(2-(2-ethyl-5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine 3-carboxamide [006051 Prepared according to the method of Example 181, substituting (R)-5-(2-(5 fluoro-2-methylpyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid with (R)-5-(2-(2-ethyl-5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxylic acid (Preparation Q). MS (apci) m/z = 395.1 (M+H). [00606] The compounds listed in Table D were prepared by the method described in Example 181 or 182, by reacting (R)-5-(2-(5-fluoro-2-methylpyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation 0) or (R)-5-(2-(2-ethyl-5 fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation Q) with an appropriate amine starting material in the presence of an amide coupling reagent (e.g. EDCI/HOBt) and an organic base (e.g. TEA) in an appropriate solvent (e.g. DCM). Table D Ex. # Structure Chemical Name Data - N (R)-N-tert-butyl-5-(2-(5-fluoro-2- LCMS (apci) 183 methylpyridin-3 -yl)pyrrolidin-1-/z = 397.1 N NH yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) o carboxamide F N-N (R)-5-(2-(5-fluoro-2-methylpyridin- LCMS (apci) 184 3-yl)pyrrolidin-1-yl)-N- m/z = 383.1 NH isopropylpyrazolo[1,5- (M+H) o a]pyrimidine-3-carboxamide F N (R)-N-cyclobutyl-5-(2-(5-fluoro-2- LCMS (apci) 185 - N methylpyridin-3-yl)pyrrolidin-1- m/z = 395.1 QCN NH yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) 0 6 carboxamide WO 2011/006074 PCT/US2010/041538 148 Ex. # Structure Chemical Name Data F NN (R)-5-(2-(5-fluoro-2-methylpyridin- LCMS (apci) 186 3-yl)pyrrolidin-1-yl)-N- = 355.1 N methylpyrazolo[1,5-a]pyrimidine- (M+H) N NH 3-carboxamide F -N N (R)-5-(2-(5-fluoro-2-methylpyridin- LCMS (apci) 187 /' N 3-yl)pyrrolidin-1-yl)pyrazolo[1,5- m/z = 341.0 N NH 2 a]pyrimidine-3-carboxamide (M+H) F -N (R)-5-(2-(5-fluoro-2-methylpyridin- LCMS (apci) 188 N 3-yl)pyrrolidin-1-yl)-N-(2- = 385.1 O NH hydroxyethyl)pyrazolo[1,5- M+H) OH a]pynimidine-3-carboxamide F -N (R)-5-(2-(5-fluoro-2-methylpyridin- LCMS (apci) 189 , r 3-yl)pyrrolidin-1 -yl)-N-((R)-2- m = 399.1 O NH hydroxypropyl)pyrazolo[1,5- (M+H) OH a pyimidine-3-cabOXamide MH _ _ OH F N-N (R)-5-(2-(5-fluoro-2-methylpyridin- LCMS (apci) 190 3-yl)pyrrolidin-1-yl)-N-(1- m/z = 395.1 N NH methylcyclopropyl)pyrazolo[1,5- (M+H) o a]pyrimidine-3-carboxamide F N-N (R)-5-(2-(5-fluoro-2-methylpyridin- LCMS (apci) 191 N 3-yl)pyrrolidin-1-yl)-N-(2- = 399.1 O NH methoxyethyl)pyrazolo[1,5- M+H) 0 \- a]pyrimidine-3-carboxamide (M+H) F (R)-(5-(2-(5-fluoro-2 N N methylpyridin-3-yl)pyrrolidin-1- LCMS (apci) 192 N yl)pyrazolo[1,5-a]pyrimidin-3- m/z = 397.1 SN OH yl)(3-hydroxyazetidin-1- (M+H) yl)methanone (R)-5-(2-(5-fluoro-2-methylpyridin N N\ 3-yl)pyrrolidin- 1 -yl)-N-(1- LCMS (apci) 193 N (hydroxymethyl)cyclopropyl)pyraz m/z = 411.1 O olo[1,5-a]pyrimidine-3- (M+H) OH carboxamide WO 2011/006074 PCT/US2010/041538 149 Ex. # Structure Chemical Name Data N F N-N 5-((R)-2-(5-fluoro-2-methylpyridin- LCMS (apci) 194 (JN N N H 3-yl)pyrrolidin-1-yl)-N-((trans)-4- m/z = 439.1 o hydroxycyclohexyl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide OH F N-N2 5-((R)-2-(5-fluoro-2-methylpyridin- LCMS (apci) 195 N N N H 3-yl)pyrrolidin-1-yl)-N-((cis)-4- m/z = 439.2 O hydroxycyclohexyl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide OH F N 5-((R)-2-(5-fluoro-2-methylpyridin 196 N 3-yl)pyrrolidin-1-yl)-N-((1S,3S)-3 196 N N hydroxycyclopentyl)pyrazolo[1,5- m/z = 425.1 0 OQOH a]pyrimidine-3-carboxamide (M+H) F N-N 5-((R)-2-(5-fluoro-2-methylpyridin 197 N 3-yl)pyrrolidin-1-yl)-N-((1R,2R)-2- LCMS (apci) O NH H hydroxycyclopentyl)pyrazolo[1,5- (M+H) 0 a]pyrimidine-3-carboxamide rF 5-((R)-2-(5-fluoro-2-methylpyridin- LCMS (apci) 198 N 3-yl)pyrrolidin-1-yl)-N-((R)- m/z = 450.2 N ~N N quinuclidin-3-yl)pyrazolo[1,5- (M+H) S 0 H a]pyrimidine-3-carboxamide F N -N 5-((R)-2-(2-ethyl-5-fluoropyridin 199 N N 3-yl)pyrrolidin-1-yl)-N-((trans)-4- = 453.2 o hydroxycyclohexyl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide OH F 5-((R)-2-(2-ethyl-5-fluoropyridin 200 N 3-yl)pyrrolidin-1-yl)-N-((1S,3S)-3- LCMS (apci) 0N NH hydroxycyclopentyl)pyrazolo[1,5- m/z = 439.1 a]pyrimidine-3-carboxamide F (R)-5-(2-(2-ethyl-5-fluoropyridin 201 N 3-yl)pyrrolidin-1-yl)-N-(2-hydroxy CN N N 2-methylpropyl)pyrazolo[1,5- (M+H) OH a]pyrimidine-3-carboxamide WO 2011/006074 PCT/US2010/041538 150 Example 202 F HN N N NH 0 (R)-N-tert-butyl-5-(2-(5-fluoro-2-oxo-1,2-dihydropyridin-3-Vl)pyrrolidin-1-Vl)pyrazolo[1,5 aklyrimidine-3-carboxamide. [006071 A pressure flask was charged with (R)-N-tert-butyl-5-(2-(5-fluoro-2 methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide (Example 80, 10 mg, 0.024 mmol), dioxane (0.7 mL) and 2M HCl (0.100 mL, 0.200 mmol). The flask was sealed and the reaction mixture was stirred at 80 0 C for 5 days. The mixture was cooled to ambient temperature and concentrated. The residue was purified by reverse-phase column chromatography (0-50% acetonitrile/water) to afford the title compound (8.2 mg, 85%). MS (apci) m/z = 399.1 (M+H). Example 203 F ONH (R)-N-(2-chloroethyl)-5-(2-(5-fluoro-2-oxo-1,2-dihydropyridin-3-vl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00608] Prepared by the method described in Example 202, replacing (R)-N-tert-butyl 5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxamide with (R)-N-(2-chloroethyl)-5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide (Example 163, 100 mg, 0.239 mmol), replacing 2 M HCl with 4 M HCl dioxane solution, and reaction was conducted at 100 0 C for 90 minutes. LCMS (apci) m/z = 405.0 (M+H). Example 204 F 0 WO 2011/006074 PCT/US2010/041538 151 N-cyclopropyl-5-((2R)-2-(2-((2,2-dimethyl- 1,3-dioxolan-4-yl)methoxy)-5 fluorophenyl)pyrrolidin- 1 -yl)pyrazolo[ 1,5-a]lpyrimidine-3-carboxamide [00609] Step A: Preparation of ethyl 5-((2R)-2-(2-((2,2-dimethyl-1,3-dioxolan-4 yl)methoxy)-5-fluorophenvll)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3-carboxylate. A mixture of (R)-ethyl 5-(2-(5-fluoro-2-hydroxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylate (Example 86, Step C, 140 mg, 0.378 mmol), 4-(chloromethyl) 2,2-dimethyl-1,3-dioxolane (114 mg, 0.756 mmol), potassium carbonate (261 mg, 1.89 mmol) and sodium bromide (77.8 mg, 0.756 mmol) in dry DMF (5 mL) was heated at 100 0 C for 14 days. The mixture was concentrated and the residue purified by chromatography to afford the title compound (45 mg, 25 % yield). MS (apci) m/z = 485.0 (M+H). [00610] Step B: Preparation of 5-((2R)-2-(2-((2,2-dimethyl-1,3-dioxolan-4 yl)methoxy)-5-fluorophenvll)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. The compound was prepared according to the method of Example 86, Step E, using ethyl 5 ((2R)-2-(2-((2,2-dimethyl-1,3-dioxolan-4-yl)methoxy)-5-fluorophenyl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (47 mg, 100 %). MS (apci) m/z = 457.0 (M+H). [00611] Step C: Preparation of N-cyclopropyl-5-((2R)-2-(2-((2,2-dimethyl-1,3 dioxolan-4-vl)methoxv)-5-fluorophenvll)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidine-3 carboxamide. Prepared according to the method of Example 86, Step F, using 5-((2R)-2-(2 ((2,2-dimethyl-1,3-dioxolan-4-yl)methoxy)-5-fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid and cyclopropyl amine to yield the title compound as a white solid (33.0 mg, 99% yield.). MS (apci) m/z = 496.1 (M+H). Example 205 F 0 0 4 5-((2R)-2-(2-((2,2-dimethyl-1,3-dioxolan-4-vl)methoxv)-5-fluorophenvl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00612] Prepared according to the procedure described in Example 204 using ammonium chloride in place of cyclopropyl amine in Step C (white solid, 13 mg, 85% yield.). MS (apci) m/z = 456.0 (M+H).

WO 2011/006074 PCT/US2010/041538 152 Example 206 O0 F O 0 N NH N-cyclopropyl-5-((2R)-2-(3-((2,2-dimethyl-1,3-dioxolan-4-yl)methoxy)-5 fluorophenyl)yrrolidin-1-yl)pyrazolo[1,5-aklyrimidine-3-carboxamide [00613] Prepared according to the method of Example 204 using (R)-ethyl 5-(2-(3 fluoro-5-hydroxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (Example 76, Step C) in Step A (36 mg, 82% yield). MS (apci) m/z = 496.1(M+H). Example 207 o F N

NH

2 5-((2R)-2-(3-((2,2-dimethyl-1,3-dioxolan-4-vl)methoxv)-5-fluorophenvl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00614] Prepared according to the method of Example 204, using (R)-ethyl 5-(2-(3 fluoro-5-hydroxyphenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (Example 76, Step C) in Step A and ammonium chloride in Step C to yield the title compound as a white solid (19 mg, 55% yield.). MS (apci) m/z = 456.0 (M+H). Example 208 HO F HOO N NH N-cyclopropyl-5-((2R)-2-(3-(2,3-dihydroxvpropoxv)-5-fluorophenvl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [006151 A solution of N-cyclopropyl-5-((2R)-2-(3-((2,2-dimethyl-1,3-dioxolan-4 yl)methoxy)-5-fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide (Example 206, 30 mg, 0.061 mmol) in dioxane (0.5 mL) was charged with two drops of 6N HCl and shaken for two minutes. DIEA (5 drops) was added and the mixture directly WO 2011/006074 PCT/US2010/041538 153 purified by reverse phase column chromatography (0-50% acetonitrile/water) to afford N cyclopropyl-5-((2R)-2-(3-(2,3-dihydroxypropoxy)-5-fluorophenyl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide (23 mg, 83 % yield) as a clear film. MS (apci) m/z = 456.1 (M+H). Example 209 HO F HO N NH 2 5-((2R)-2-(3-(2,3-dihydroxvpropoxv)-5-fluorophenvll)pyrrolidin-1-vl)pyrazolo[1,5 alpyrimidine-3-carboxamide [00616] Prepared from 5-((2R)-2-(3-((2,2-dimethyl-1,3-dioxolan-4-yl)methoxy)-5 fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide (Example 207) according to the procedure of Example 208 (8.5 mg, 55 % yield). MS (apci) m/z = 416.0 (M+H). Example 210 F HO OH N NH N-cyclopropyl-5-((2R)-2-(2-(2,3-dihydroxvpropoxv)-5-fluorophenvl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [006171 Prepared from N-cyclopropyl-5-((2R)-2-(2-((2,2-dimethyl-1,3-dioxolan-4 yl)methoxy)-5-fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide (Example 204) using the procedure of Example 208 (19 mg, 65 % yield). MS (apci) m/z = 456.1 (M+H). Example 211 F H HO O K N

NH

2 WO 2011/006074 PCT/US2010/041538 154 5-((2R)-2-(2-(2,3-dihydroxvpropoxv)-5-fluorophenvll)pyrrolidin- 1 -vl)pyrazolo[ 1,5 a]pyrimidine-3-carboxamide [00618] Prepared from 5-((2R)-2-(3-((2,2-dimethyl-1,3-dioxolan-4-yl)methoxy)-5 fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide (Example 205) using the procedure of Example 208 (10 mg, 95% yield). MS (apci) m/z = 416.0 (M+H). Example 212 F N N N HO )-CF3 5-((2R,5S)-2-(5-fluoropyridin-3-yl)-5-(hydroxymethyl)yrrolidin-1-yl)-N-((R)-1,1,1 trifluoropropan-2-vl)pyrazolo[1,5-alpyrimidine-3-carboxamide. [00619] Step A: Preparation of (S)-ethyl 2-(tert-butoxvcarbonylamino)-5-(5 fluoropyridin-3-yl)-5-oxopentanoate. A solution of 3-bromo-5-fluoropyridine (4.28 g, 24.3 mmol) in dry THF (25 mL) was cooled to -40 to -50 0 C and 2M isopropylmagnesium chloride in THF (10.2 mL, 20.4 mmol) was added. The mixture was allowed to warm to 0 0 C and was stirred for 30 minutes. The mixture was cooled to -20 0 C and a solution of (S)-1 tert-butyl 2-ethyl 5-oxopyrrolidine-1,2-dicarboxylate (5.00 g, 19.4 mmol) in dry THF (15 mL) was added. The mixture was allowed to reach ambient temperature over 30 minutes and was stirred at ambient temperature for 30 minutes. The reaction was slowly quenched with 2M HCl (10 mL, 20.0 mmol) followed by 10% aqueous NH 4 Cl (10 mL). The mixture was stirred for 10 minutes and was transferred to a separatory funnel using MTBE rinses (10 mL). Heptane (15 mL) was added and the organic layer was removed. The organic layer was washed 10% aqueous NH 4 Cl (25 mL) and DI H 2 0 (25 mL). The organic layer was concentrated to provide the crude product as a yellow oil (7.03 g, 102 %). [00620] Step B: Preparation of (2S,5R)-ethyl 5-(5-fluoropyridin-3-yl)pyrrolidine-2 carboxylate. (S)-ethyl 2-(tert-butoxycarbonylamino)-5-(5-fluoropyridin-3-yl)-5 oxopentanoate (4.80 g, 13.55 mmol) was treated with TFA (24 mL) and the mixture was stirred at ambient temperature for 45 minutes. The mixture was concentrated and the residue was dissolved in H 2 0 (10 mL) and EtOAc (50 mL) was added. The mixture was treated slowly with saturated aqueous K 2

CO

3 (15 mL). The aqueous layer was separated and the organic layer was washed with 10 % aqueous NH 4 Cl. The EtOAc layer was concentrated to WO 2011/006074 PCT/US2010/041538 155 give crude (S)-ethyl 5-(5-fluoropyridin-3-yl)-3,4-dihydro-2H-pyrrole-2-carboxylate as an amber oil (2.67 g, 83 % yield). The oil was dissolved in isopropyl alcohol (20 mL) and was treated with 10% Pd/C (0.266 g, 0.250 mmol). The reaction vessel was purged with hydrogen gas (3X) and mixture was stirred at ambient temperature under 1 atm of hydrogen for 16 hours. The reaction was purged with nitrogen and filtered through a Celite pad. The filtrate was concentrated to furnish the title compound as an amber oil that began to solidify upon standing (2.58 g, 96%). [00621] Step C: Preparation of ((2S,5R)-5-(5-fluoropyridin-3-vl)pyrrolidin-2 yl)methanol. To a solution of (2S,5R)-ethyl 5-(5-fluoropyridin-3-yl)pyrrolidine-2 carboxylate (1.10 g, 4.62 mmol) in dry THF (20 mL) was added 2M LiAlH 4 in THF (3.00 mL, 6.00 mmol). The reaction was stirred at ambient temperature for 30 minutes and Na 2

SO

4 10H 2 0 (3.00 g, 9.31 mmol) was added in small portions. The mixture was stirred for 3 hours at ambient temperature and was filtered. The collected solid was washed with EtOAc and the washes combined with the filtrate. The solution was concentrated to provide crude ((2S,5R) 5-(5-fluoropyridin-3-yl)pyrrolidin-2-yl)methanol (0.95 g, 105 % yield) that was used directly in the next step. MS (apci) m/z = 197.1 (M+H). [00622] Step D: Preparation of ethyl 5-((2R,5S)-2-(5-fluoropyridin-3-vl)-5 (hydroxymethyl)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3-carboxylate. A mixture of ((2S,5R)-5-(5-fluoropyridin-3-yl)pyrrolidin-2-yl)methanol (0.910 g, 4.64 mmol), ethyl 5 chloropyrazolo[1,5-a]pyrimidine-3-carboxylate (Preparation B, 1.05 g, 4.64 mmol) and DIlEA (1.10 mL, 6.00 mmol) in isopropyl alcohol (1.0 mL) was heated at 90 'C for 16 hours. The reaction mixture was concentrated and the residue was purified by reverse phase column chromatography (0-50% acetonitrile/water) followed by normal phase column chromatography (2-5% MeOH/DCM) to afford ethyl 5-((2R,5S)-2-(5-fluoropyridin-3-yl)-5 (hydroxymethyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (0.250 g, 14 % yield) as a viscous, clear oil. MS (apci) m/z = 386.0 (M+H). [00623] Step E: Preparation of 5-((2R,5S)-2-(5-fluoropyridin-3-vl)-5 (hydroxymethyl)pyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. A mixture of ethyl 5-((2R,5S)-2-(5-fluoropyridin-3-yl)-5-(hydroxymethyl)pyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylate (0.250 g, 0.649 mmol) and 2M sodium hydroxide (3.24 mL, 6.48 mmol) in MeOH (10 mL) was stirred at ambient temperature for 4 days followed by 50 'C for 5 hours. The reaction was cooled to ambient temperature and 4M HCl in dioxane (1.78 mL, 7.14 mmol) was added. The mixture was concentrated and the residue was purified by WO 2011/006074 PCT/US2010/041538 156 reverse phase column chromatography ( 0

-

4 0 % acetonitrile/water) to afford 5-((2R,5S)-2-(5 fluoropyridin-3-yl)-5-(hydroxymethyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxylic acid (210 mg, 90 % yield) as a white solid. MS (apci) m/z = 358.0 (M+H). [00624] Step F: Preparation of 5-((2R,5S)-2-(5-fluoropyridin-3-yl)-5 (hydroxymethyl)yrrolidin-1-yl)-N-((R)-1,1,1-trifluoropropan-2-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide. To a mixture of 5-((2R,5S)-2-(5-fluoropyridin-3-yl)-5 (hydroxymethyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (10.0 mg, 0.028 mmol), (R)-1,1,1-trifluoropropan-2-amine (6.33 mg, 0.056 mmol) and HATU (10.5 mg, 0.045 mmol) in dry DMF (0.2 mL) was added DILEA (15.0 gL, 0.084 mmol). The reaction vessel was flushed with nitrogen, sealed, and the reaction stirred at ambient temperature for 16 hours. The reaction mixture was directly purified by reverse phase column chromatography (0-50% CH 3

CN/H

2 0) to provide the title compound (6.50 mg, 51 % yield) as a white solid. MS (apci) m/z = 453.0 (M+H). Example 213 F N N YN NH / , CF 3 HO 5-((2R,5S)-2-(5-fluoropyridin-3-yl)-5-(hydroxymethyllpyrrolidin-1-yl)-N-((S)-1,1,1 trifluoropropan-2-yl)pyrazolo[1,5-aklyrimidine-3-carboxamide [006251 Prepared according to the method of Example 212, Step F, using (S)-1,1,1 trifluoropropan-2-amine (white solid; 5.5 mg, 43%). MS (apci) m/z = 453.0 (M+H). Example 214 F N NH HN HO 5-((2R,5S)-2-(5-fluoropyridin-3-yl)-5-(hydroxymethyllpyrrolidin-1-vl)-N-(1 methylcyclopropyllpyrazolo[1,5-alpyrimidine-3-carboxamide [00626] Prepared according to the method of Example 212, Step F, using 1 methylcyclopropane amine (white solid; 2.5 mg, 22%). MS (apci) m/z = 411.1 (M+H).

WO 2011/006074 PCT/US2010/041538 157 Example 215 F ~N NH HO 5-((2R,5S)-2-(5-fluoropyridin-3-yl)-5-(hydroxymethyl)pyrrolidin-1-yl-N isopropylpyrazolo[1,5-alpyrimidine-3-carboxamide [006271 Prepared according to the method of Example 212, Step F, using isopropyl amine (white solid; 2.5 mg, 12%). MS (apci) m/z = 399.1 (M+H). Example 216 F ' 0 N NH HO / CF 3 5-((2R,4S)-2-(3-fluorophenyl)-4-hydroxypyrrolidin-1-yl)-N-((S)-1,1,1-trifluoropropan-2 yllpyrazolo[1,5-alpyrimidine-3-carboxamide [00628] Step A: Preparation of (ethyl 5-((2R,4S)-4-acetoxy-2-(3 fluorophenylpyrrolidin-1-Vllypyrazolo[1,5-alpyrimidine-3-carboxylate. To a mixture of ethyl 5-((2R,4R)-2-(3-fluorophenyl)-4-hydroxypyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3 carboxylate (Example 41, Step E, 260 mg, 0.702 mmol) and PPh 3 (460 mg, 1.75 mmol) in THF (10.0 mL) was added DIAD (276 gL, 1.40 mmol) followed by acetic acid (80.4 gL, 1.40 mmol). The reaction was stirred at ambient temperature for 48 hours and then concentrated. The residue was purified by reverse phase column chromatography (0-70% acetonitrile/water) to afford ethyl 5-((2R,4S)-4-acetoxy-2-(3-fluorophenyl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (228 mg, 79% yield). MS (apci) m/z = 413.0 (M+H). [00629] Step B: Preparation of 5-((2R,4S)-2-(3-fluorophenyl)-4-hydroxypyrrolidin-1 Vllpyrazolo[1,5-alpyrimidine-3-carboxylic acid. A mixture of ethyl 5-((2R,4S)-4-acetoxy-2 (3-fluorophenyl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (225 mg, 0.546 mmol) and NaOH (131 mg, 1.64 mmol) in MeOH (1.0 mL) was stirred at ambient temperature for 60 hours followed by 3 hours at 60 0 C. The mixture was cooled to ambient temperature and 4M HCl in dioxane (1 mL) was added. The mixture was concentrated and WO 2011/006074 PCT/US2010/041538 158 the residue was treated with DCM. The mixture was filtered through Celite and concentrated to afford 5-((2R,4S)-2-(3-fluorophenyl)-4-hydroxypyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine 3-carboxylic acid (188 mg, 10 % yield) as a white solid. MS (apci) m/z = 343.0 (M+H). [00630] Step C: Preparation of 5-((2R,4S)-2-(3-fluorophenvl)-4-hydroxvpyrrolidin-1 yl)-N-((S)-1,1,1-trifluoropropan-2-yl)pyrazolo[1,5-alpyrimidine-3-carboxamide. Prepared according to the method of Example 212, Step F, using 5-((2R,4S)-2-(3-fluorophenyl)-4 hydroxypyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid and (S)-1,1,1 trifluoropropan-2-amine (white solid; 2.1 mg, 16 % yield). MS (apci) m/z = 438.0 (M+H). Example 217 F N NH HO 5-((2R,4S)-2-(3-fluorophenyl)-4-hydroxypyrrolidin-1-yl)-N-isopropylpyrazolo[1,5 alpyrimidine-3-carboxamide [00631] Prepared according to the method of Example 212, Step F, using 5-((2R,4S) 2-(3-fluorophenyl)-4-hydroxypyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Example 216, Step B) and isopropyl amine. The title compound was obtained as a white solid (5.5 mg, 49 % yield). MS (apci) m/z = 384.1 (M+H). Example 218 F N oNH HO 5-((2R,4S)-2-(3-fluorophenyl)-4-hydroxypyrrolidin-1-yl)-N-methylpyrazolo[1,5 alpyrimidine-3-carboxamide [00632] Prepared according to the method of Example 212, Step F, using 5-((2R,4S) 2-(3-fluorophenyl)-4-hydroxypyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Example 216, Step B) and methyl amine. The title compound was obtained as a white solid (6.1 mg, 29 % yield). MS (apci) m/z = 356.1 (M+H).

WO 2011/006074 PCT/US2010/041538 159 Example 219 F N N - N NH - 0 HO 1 5-((2S,5R)-5-(5-fluoropyridin-3-yl)-2-(hydroxymethyl)-2-methylpyrrolidin-1-yl)-N isopropylpyrazolo[1,5-alpyrimidine-3-carboxamide [00633] Step A: Preparation of (2S,5R)-1-tert-butyl 2-ethyl 5-(5-fluoropyridin-3 yl)yrrolidine- 1,2-dicarboxylate. A mixture of (2S,5R)-ethyl 5-(5-fluoropyridin-3 yl)pyrrolidine-2-carboxylate (Example 212, Step B, 1.00 g, 4.20 mmol), di-tert-butyl dicarbonate (0.962 g, 4.41 mmol) and PS-DMAP (0.210 g, 0.210 mmol, 1.00 mmol/g load) in dry DCM (20 mL) was mixed at ambient temperature for 18 hours. The reaction mixture was filtered and the filtrate was concentrated. The residue was purified on a silica gel column (2 10% MeOH/DCM) to afford (2S,5R)-1-tert-butyl 2-ethyl 5-(5-fluoropyridin-3-yl)pyrrolidine 1,2-dicarboxylate (1.36 g, 96 % yield) as a yellow oil. MS (apci) m/z = 339.0 (M+H). [00634] Step B: Preparation of (2S,5R)-1-tert-butyl 2-ethyl 5-(5-fluoropyridin-3-vl)- 2 methylpyrrolidine- 1,2-dicarboxylate. A solution of (2S,5R)-1-tert-butyl 2-ethyl 5-(5 fluoropyridin-3-yl)pyrrolidine-1,2-dicarboxylate (250 mg, 0.739 mmol) in THF (10 mL) was cooled to -78 'C and 0.5 M KHMDS in toluene (1.77 mL, 0.885 mmol) was added dropwise. The reaction was stirred for 1 hour at -78 'C and Mel (59.9 gL, 0.960 mmol) was added. The reaction was allowed to warm to ambient temperature and saturated aqueous NaCl (20 mL) was added. The mixture was extracted with EtOAc (2 x 50 mL) and the combined organic extracts were filtered and concentrated to afford (2S,5R)-1-tert-butyl 2-ethyl 5-(5 fluoropyridin-3-yl)-2-methylpyrrolidine-1,2-dicarboxylate (255 mg, 98 % yield) as a clear oil. MS (apci) m/z = 353.1 (M+H). [006351 Step C: Preparation of (2S,5R)-tert-butyl 5-(5-fluoropyridin-3-yl)- 2 (hydroxymethyl)-2-methylpyrrolidine-1-carboxylate. A solution of (2S,5R)-1-tert-butyl 2 ethyl 5-(5-fluoropyridin-3-yl)-2-methylpyrrolidine-1,2-dicarboxylate (240 mg, 0.681 mmol) in THF (10 mL) was cooled to -78 'C and IM LiAlH 4 in THF (1.50 mL, 1.50 mmol) was added dropwise. The reaction was allowed to warm to 0 'C and was quenched with small portions of Na 2

SO

4 -10H 2 0 (967 mg, 6.81 mmol). The mixture was filtered and concentrated WO 2011/006074 PCT/US2010/041538 160 to afford (2S,5R)-tert-butyl 5-(5-fluoropyridin-3-yl)-2-(hydroxymethyl)-2-methylpyrrolidine 1-carboxylate (200 mg, 95% yield). MS (apci) m/z = 311.1 (M+H). [00636] Step D: Preparation of ((2S,5R)-5-(5-fluoroyridin-3-yl)-2-methylpyrrolidin 2-yl)methanol hydrochloride. To a solution of (2S,5R)-tert-butyl 5-(5-fluoropyridin-3-yl)-2 (hydroxymethyl)-2-methylpyrrolidine-1-carboxylate (200 mg, 0.644 mmol) in DCM (5 mL) was added 4M HCl in dioxane (1.61 mL, 6.44 mmol). The reaction was stirred at ambient temperature for 16 hours and then concentrated to afford ((2S,5R)-5-(5-fluoropyridin-3-yl)-2 methylpyrrolidin-2-yl)methanol hydrochloride (130 mg, 96 % yield). MS (apci) m/z = 211.1 (M+H). [006371 Step E: Preparation of ethyl 5-((2S,5R)-5-(5-fluoropyridin-3-yl)-2 (hydroxymethyl)-2-methylpyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylate. A sealed reaction vessel was charged with ((2S,5R)-5-(5-fluoropyridin-3-yl)-2 methylpyrrolidin-2-yl)methanol hydrochloride (0.135 g, 0.547 mmol), ethyl 5 chloropyrazolo[1,5-a]pyrimidine-3-carboxylate (Preparation B, 0.136 g, 0.602 mmol), DIlEA (0.124 mL, 0.711 mmol) and isopropyl alcohol (1 mL). The vessel was sealed and the mixture was heated at 190 'C for 48 hours. The reaction was cooled to ambient temperature and concentrated. The residue was purified by reverse phase column chromatography (0 50% acetonitrile/water) to afford ethyl 5-((2S,5R)-5-(5-fluoropyridin-3-yl)-2 (hydroxymethyl)-2-methylpyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (55 mg, 25 % yield) as a viscous clear oil. MS (apci) m/z = 400.1 (M+H). [00638] Step F: Preparation of 5-((2S,5R)-5-(5-fluoropyridin-3-yl)-2 (hydroxymethyl)-2-methylpyrrolidin-1-yl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. A mixture of ethyl 5-((2S,5R)-5-(5-fluoropyridin-3-yl)-2-(hydroxymethyl)-2-methylpyrrolidin 1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate (52.0 mg, 0.130 mmol) and NaOH (26.0 mg, 0.325 mmol) in MeOH (1.0 mL) was stirred at ambient temperature for 60 hours. The reaction was treated with HCl (4 N dioxane, 163 gL, 0.651 mmol) and concentrated. The residue was treated with DCM and filtered through Celite. The solution was concentrated to afford 5-((2S,5R)-5-(5-fluoropyridin-3-yl)-2-(hydroxymethyl)-2-methylpyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (29 mg, 60 % yield) as a white solid. MS (apci) m/z = 372.0 (M+H). [00639] Step G: Preparation of 5-((2S,5R)-5-(5-fluoropyridin-3-yl)-2 (hydroxymethyl)-2-methylpyrrolidin-1-yl)-N-isopropylpyrazolo[1,5-a]lpyrimidine-3 carboxamide. Prepared according to the method of Example 212, Step F, using 5-((2S,5R)-5- WO 2011/006074 PCT/US2010/041538 161 (5-fluoropyridin-3-yl)-2-(hydroxymethyl)-2-methylpyrrolidin- 1 -yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid and isopropyl amine. The title compound was obtained as a white solid (7.2 mg, 46 % yield). MS (apci) m/z = 413.1 (M+H). Example 220 F N N CNCF NH H OOK HO~ ~ CF 3 5-((2S,5R)-5-(5-fluoropyridin-3-vl)-2-(hydroxymethyl)-2-methylpyrrolidin-1-yl)-N-((S) 1,1,1-trifluoropropan-2-yl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00640] Prepared according to the method of Example 212, Step F, using 5-((2S,5R) 5-(5-fluoropyridin-3-yl)-2-(hydroxymethyl)-2-methylpyrrolidin-1-yl)pyrazolo[1,5 a]pyrimidine-3-carboxylic acid (Example 219, Step F) and (S)-1,1,1-trifluoropropan-2-amine. The title compound was obtained as a white solid (5.4 mg, 31 % yield). MS (apci) m/z = 467.1 (M+H). Example 221 N\ NO H2N ' N (R)-(5-(2-(2-amino-5-fluoropyridin-3-vl)pyrrolidin-1-vl)pyrazolo[1,5-alpyrimidin-3 yl)(azetidin- 1 -yl)methanone [00641] A mixture of (R)-N-(3-aminopropyl)-5-(2-(5-fluoro-2-oxo-1,2 dihydropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide hydrochloride (Example 142, 83 mg, 0.190 mmol) and POCl 3 (697 gL, 7.62 mmol) was sealed and heated at 100 'C for 5 minutes. The reaction mixture was diluted with 1 mL heptane and azeotroped twice to yield the crude product. The crude material was purified by reverse-phase chromatography (5 to 40% acetonitrile/water) to yield the title product as white solid (2 mg, 3%). LCMS (apci) m/z = 382.3 (M+H).

WO 2011/006074 PCT/US2010/041538 162 Example 222 F N F N 0 N HN Ci N NH (R)-tert-butyl 3-(5-(2-(2-chloro-5-fluoroyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamido)propylcarbamate [00642] Step A: Preparation of (R)-tert-butyl 2-(2-chloro-5-fluoropyridin-3 vl)pyrrolidine-1-carboxylate: A solution of tert-butyl pyrrolidine-1-carboxylate (1 mL 5.70 mmol) and (-)-sparteine (1.31 mL, 5.70 mmol) in anhydrous MTBE (30 mL) was first cooled to -78 0 C under nitrogen, followed by addition of sec-butyl lithium (4.07 mL, 1.4M, 5.70 mmol) drop-wise over 15 minutes with a syringe, maintaining the temperature below -75 0 C. The pale yellowish solution was stirred at -78 0 C for 3 hours before being treated with zinc chloride (3.80 mL, 1.0 M, 3.80 mmol) dropwise over 15 minutes while maintaining the temperature below -73 0 C. The mixture was stirred at -78 0 C for 30 minutes, then placed into an ambient temperature water bath and stirred for another hour. At this point a large amount of white precipitate was present. The mixture was treated with 3-bromo-2-chloro-5 fluoropyridine (1.00 g, 4.75 mmol) in MTBE (5 mL), followed by addition of palladium acetate (53 mg, 0.24 mmol) and tri-t-butylphosphine tetrafluoroborate (83 mg, 0.28 mmol). The mixture was allowed to stir at ambient temperature overnight to reach completion. The mixture was treated with NH 4 0H (1 mL), stirred for 30 minutes and filtered through GF/F paper, washing with MTBE. The filtrate was washed with 10% citric acid (30 mL) and the aqueous layer was back-washed with MTBE (2 x 30 mL). The combined organic phases were washed with brine (20 mL), dried (MgSO 4 ), and concentrated to afford the crude product as dark yellowish oil. This crude material was purified on a silica 50 g Biotage SNAP cartridge eluting with 10% EtOAc in hexanes to afford the desired product as colorless oil (0.5 g, 35%). MS (apci pos) m/z = 201.1 (M+H-Boc). [00643] Step B: Preparation of (R)-2-chloro-5-fluoro-3-(pyrrolidin-2-vl)pyridine dihydrochloride: To a dioxane (5 mL) solution of (R)-tert-butyl 2-(2-chloro-5-fluoropyridin 3-yl)pyrrolidine-1-carboxylate (500 mg, 1.66 mmol) was added HCl (4 N dioxane, 20 mL), followed by stirring at ambient temperature overnight. The mixture was concentrated and treated with Et 2 0, then vacuum-dried, to provide the product as a white solid (0.36 g, 80%).

WO 2011/006074 PCT/US2010/041538 163 MS (apci pos) m/z = 201.1 (M+H). The enantiomeric excess (ee%) of the product was determined to be >92% according to the method described in Preparation A. [00644] Step C: Preparation of (R)-ethyl 5-(2-(2-chloro-5-fluoropyridin-3 Vl)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3-carboxylate: To a solution of ethyl 5 hydroxypyrazolo[1,5-a]pyrimidine-3-carboxylate (Preparation B, Step A, 275 mg, 1.33 mmol) in anhydrous DMF (5 mL) was added (Benzotriazol- 1 yloxy)tris(dimethylamino)phosphonium hexafluorophosphate (BOP) (646 mg, 1.46 mmol). The heterogeneous mixture was stirred for 10 minutes before adding DIlEA (1.16 mL, 6.6 mmol), followed by addition of (R)-2-chloro-5-fluoro-3-(pyrrolidin-2-yl)pyridine dihydrochloride (363 mg, 1.33 mmol). The reaction was stirred at ambient temperature overnight to reach completion. The mixture was partitioned between 10% citric acid (30 mL) and EtOAc (30 mL), and the aqueous layer was extracted with EtOAc (2 x 20 mL). The combined organic phases were washed successively with water (20 mL), saturated NaHCO 3 (20 mL), water (20 mL) and brine (3 x 20 mL), then dried (Na 2

SO

4 ) and concentrated to afford the crude product as an orange foam. The crude material was purified on a 25 g Biotage SNAP silica cartridge eluting with 1% MeOH/DCM to afford the desired product as cream-colored foam (0.35 g, 68%). MS (apci pos) m/z = 390.0 (M+H). [006451 Step D: Preparation of (R)-5-(2-(2-chloro-5-fluoropyridin-3-vl)pyrrolidin-1 yl)pyrazolo[1,5-alpyrimidine-3-carboxylic acid. Prepared by the method described in Preparation C, Step B, replacing (R)-ethyl 5-(2-(2,5-difluorophenyl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate with (R)-ethyl 5-(2-(2-chloro-5-fluoropyridin-3 yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylate. MS (apci pos) m/z = 361.9 (M+H). [00646] Step E: Preparation of (R)-tert-butyl 3-(5-(2-(2-chloro-5-fluoropyridin-3 Vl)pyrrolidin-1-Vl)pyrazolo[1,5-alpyrimidine-3-carboxamido)propylcarbamate. Prepared according to the method described in Example 141, replacing (R)-5-(2-(5-fluoro-2 methoxypyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid with (R) 5-(2-(2-chloro-5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid to yield the title product as white solid. LCMS (apci pos) m/z = 418.2 (M+H-Boc).

WO 2011/006074 PCT/US2010/041538 164 Example 223 F N N N rO ci HN 'NNH2 0 (R)-N-(3-aminopropyl)-5-(2-(2-chloro-5-fluoropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5 alpyrimidine-3-carboxamide [006471 A mixture of (R)-tert-butyl 3-(5-(2-(2-chloro-5-fluoropyridin-3-yl)pyrrolidin 1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamido)propylcarbamate (Example 222, 6 mg, 0.012 mmol) and HCl (4 N dioxane, 145 gL, 0.58 mmol) was stirred at ambient temperature for 2 hours and concentrated to yield the product as white solid. LCMS (apci pos) m/z = 418.1 (M+H). Example 224 F N NN c H N, (R)-N-(2-tert-butoxyethoxy)-5-(2-(2-chloro-5-fluoropyridin-3-yl)pyrrolidin-1 Vl)pyrazolo[1,5-alpyrimidine-3-carboxamide [00648] Prepared according to the method described in Example 222, Step E, replacing tert-butyl 3-aminopropylcarbamate with O-(2-tert-butoxyethyl)hydroxylamine hydrochloride to yield the title product as white solid (58 mg, 87%). LCMS (apci) m/z = 476.9 (M+H). Example 225 F N N O O HN, ci 0' OH (R)-5-(2-(2-chloro-5-fluoropyridin-3-yl)pyrrolidin-1-yl)-N-(2-hydroxyethoxy)pyrazolo[1,5 alpyrimidine-3-carboxamide [00649] (R)-N-(2-tert-butoxyethoxy)-5-(2-(2-chloro-5-fluoropyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide (Example 224, 57 mg, 0.120 mmol) was treated with HCl (4 N dioxane, 1.49 mL, 5.98 mmol), followed by two drops of MeOH to make a clear colorless solution. After stirring 30 minutes at ambient temperature, the reaction was WO 2011/006074 PCT/US2010/041538 165 concentrated and dried to yield the title product as white solid, assuming quantitative yield. LCMS (apci) m/z = 421.0 (M+H) Example 226 F N O~ N NH §NNH (R)-N-tert-butyl-5-(2-(5-fluoro-1-methyl-2-oxo-1,2-dihydropyridin-3-vl)pyrrolidin-1 yl)yrazolo[1,5-alpyrimidine-3-carboxamide [006501 Prepared by the method described in Example 140, using (R)-5-(2-(5-fluoro-1 methyl-2-oxo- 1,2-dihydropyridin-3 -yl)pyrrolidin- 1 -yl)pyrazolo [1,5 -a]pyrimidine-3 carboxylic acid (Preparation R) and 2-methylpropan-2-amine. The residue was purified by silica column chromatography, eluting with 3% MeOH/DCM to yield the title compound (26 mg, 75% yield). MS (apci) m/z = 413.1 (M+H). [006511 The compounds listed in the following Table were also prepared according to the method described in Example 140, by reacting (R)-5-(2-(5-fluoro-1-methyl-2-oxo-1,2 dihydropyridin-3-yl)pyrrolidin-1-yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation R) with the appropriate amine starting material in the presence of an amide coupling reagent (e.g. EDCI/HOBt), an organic base (for example, TEA) in a solvent (for example, DCM). Table E Ex. # Structure Chemical Name Data F (R)-5-(2-(5-fluoro-1-methyl-2-oxo 22 N' 1,2-dihydropyridin-3-yl)pyrrolidin-1- 399.pci) m/z 227 4NiprypyaOO[5=399.1 CYN L NH yl)-N-isopropylpyrazolo[ 1,5- (M+H) N H a]pyrimidine-3-carboxamide F (R)-N-cyclopropyl-5-(2-(5-fluoro-1- . 228 methyl-2-oxo- 1,2-dihydropyridin-3 - 397.pci) m/z N NH yl)pyrrolidin-1-yl)pyrazolo[1,5- (M+H) 0 a]pyrimidine-3-carboxamide WO 2011/006074 PCT/US2010/041538 166 Ex. # Structure Chemical Name Data F (R)-5-(2-(5-fluoro-1-methyl-2-oxo 1,2-dihydropyridin-3-yl)pyrrolidin-1- MS (apci) m/z 229 C N I NH yl)-N-(6-methylpyridin-3- = 448.1 o / yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) N' carboxamide F N (R)-N-cyclobutyl-5-(2-(5-fluoro-1 230 methyl-2-oxo-1,2-dihydropyridin-3 N3NHNyl)pyrrolidin- 1 -yl)pyrazolo [1,5 - (M+H) o H a]pyrimidine-3-carboxamide /I F, N-N (R)-5-(2-(5-fluoro-1 -methyl-2-oxo 231,2-dihydropyridin-3-yl)pyrrolidin-1- 43apci) m/z 231 NH yl)-N-(pyridin-3-yl)pyrazolo[1,5- 434.1 a]pyrimidine-3-carboxamide (M+H) F (R)-N-(cyclopropylmethyl)-5-(2-(5 o fluoro-1I-methyl-2-oxo- 1,2- MS (apci) mlz 232 N dihydropyridin-3 -yl)pyrrolidin- 1- 411.1 O NH yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) carboxamide N F5-((R)-2-(5-fluoro-1 -methyl-2-oxo - 1,2-dihydropyridin-3-yl)pyrrolidin- 1- MS (apci) m/z 233 (yN yl)-N-((S)-1-hydroxy-3,3- = 457.1 , OH dimethylbutan-2-yl)pyrazolo[1,5- (M+H) _ _ __ a]pyrimidine-3-carboxamide N F 5-((R)-2-(5-fluoro-1-methyl-2-oxo O 1,2-dihydropyridin-3-yl)pyrrolidin-1- MS (apci) m/z 234 CyN N yl)-N-((1R,2R)-2- = 455.1 O ). OH hydroxycyclohexyl)pyrazolo[1,5- (M+H) a]pyrimidine-3-carboxamide N F N-((R)-1-cyclopropylethyl)-5-((R)-2 (5-fluoro-1-methyl-2-oxo-1,2- MS (apci) m/z 235 dihydropyridin-3 -yl)pyrrolidin- 1- = 425.1 NH yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) carboxamide N F N-((S)-1-cyclopropylethyl)-5-((R)-2 (5-fluoro-1-methyl-2-oxo-1,2- MS (apci) m/z 236 dihydropyridin-3 -yl)pyrrolidin- 1- = 425.1 NH yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) _ _ carboxamide WO 2011/006074 PCT/US2010/041538 167 Ex. # Structure Chemical Name Data F (R)-5-(2-(5-fluoro-1-methyl-2-oxo 1,2-dihydropyridin-3-yl)pyrrolidin-1- MS (apci) m/z 237 .. yl)-N-(1 - =411.1 23 N NH methylcyclopropyl)pyrazolo[1,5- (M+H) o a]pyrimidine-3-carboxamide -N 5-((R)-2-(5-fluoro-1-methyl-2-oxo 0 1,2-dihydropyridin-3-yl)pyrrolidin-1- MS (apci) m/z 238 N NH yl)-N-((trans)-4- = 455.1 O ~ hydroxycyclohexyl)pyrazolo[1,5- (M+H) b. a]pyrimidine-3-carboxamide 6H FN-N (R)-5-(2-(5-fluoro-1-methyl-2-oxo o N" 1,2-dihydropyridin-3-yl)pyrrolidin-1- MS (apci) m/z 239 N NH yl)-N-(5-fluoropyridin-2- = 452.1 O N~ yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) carboxamide Example 240 F I N N -o H N Me (R)-5-(2-(5-fluoro-2-methoxyVpyridin-3-Vl)pyrrolidin-1-yl)-N-(3-methyl-iH-pyrazol-5 yl)yrazolo[1,5-alpyrimidine-3-carboxamide [00652] To a suspension of (R)-5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid (Preparation K, 101 mg, 0.283 mmol) in THF (5 mL) was added triethylamine (34.3 mg, 0.339 mmol), followed by the addition of 2,4,6 trichlorobenzoyl chloride (75.8 mg, 0.311 mmol). The suspension was stirred for 2 hours and then 3-methyl-1H-pyrazol-5-amine (35.7 mg, 0.367 mmol) was introduced. The reaction was heated at 60 0 C for 3 hours. After cooling to room temperature, the reaction was partitioned between EtOAc (20 mL) and saturated aqueous NaHCO 3 (10 mL). After phase-separation, the aqueous layer was extracted with EtOAc (2 x 10 mL). The combined organics were dried (Na 2 SO4), filtered and concentrated. The residue was purified via silica chromatography WO 2011/006074 PCT/US2010/041538 168 (EtOAc/MeOH 20:1) to yield the title product (23 mg, 19 %). LCMS (apci) m/z = 437.0 (M+H). [00653] The compounds listed in Table F were also prepared according to the method described in Example 240, using (R)-5-(2-(5-fluoro-2-methoxypyridin-3-yl)pyrrolidin-1 yl)pyrazolo[1,5-a]pyrimidine-3-carboxylic acid, Preparation K) and an appropriate amine. Table F Ex. # Structure Chemical Name Data ,N (R)-5-(2-(5-fluoro-2 241 U 0 methoxypyridin-3-yl)pyrrolidin-1- LCMS (apci) N N yl)-N-(1-methyl-1H-pyrazol-3- m/z = 437.0 -O N HN N yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) N- carboxamide ,N (R)-N-(3-cyclopropyl-1H-pyrazol 242 N §N 5-yl)-5-(2-(5-fluoro-2- LCMS (apci) -O N H NH methoxypyridin-3-yl)pyrrolidin-1- m/z = 463.0 yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) carboxamide F N N N O (R)-N-(3 -ethyl-i H-pyrazol-5 -yl)-5 - LCMS (apci) 243 N N H N (2-(5-fluoro-2-methoxypyridin-3- m = 451.0 --- NH yl)pyrrolidin-1-yl)pyrazolo[1,5- (M+H) / a]pyrimidine-3-carboxamide Et ,N (R)-5-(2-(5-fluoro-2 24 N N O methoxypyridin-3 -yl)pyrrolidin-1I- LCMS (apci) N H N yl)-N-(1-isopropyl-1H-pyrazol-3- m/z = 465.0 ~~~ rN yl)pyrazolo[1,5-a]pyrimidine-3- (M+H) carboxamide [00654] The compounds in Table G can also be prepared according to the method of Example 240.

WO 2011/006074 PCT/US2010/041538 169 Table G Ex. # Structure Name 245 F ,N (R)-5-(2-(5-fluoro-2-methoxypyridin 3-yl)pyrrolidin-1-yl)-N-(2-methyl-1H N #N imidazol-4-yl)pyrazolo[1,5 '- N HN a]pyrimidine-3-carboxamide IN H 246 F N (R)-N-(1,2-dimethyl- 1 H-imidazol-4 / yl)-5-(2-(5-fluoro-2-methoxypyridin-3 N\ -N O yl)pyrrolidin- 1 -yl)pyrazolo[ 1,5 N HN N a]pyrimidine-3-carboxamide ..- 0

N

Claims (63)

1. A compound having the general formula I R 3 NN >N N R1 (4), (R )n~)N x 0 R 2 5 or a salt thereof, wherein: R 1 is H or (1-6C alkyl); R2 is H, (1-6C)alkyl, -(1-6C)fluoroalkyl, -(1-6C)difluoroalkyl, -(1-6C)trifluoroalkyl, (1-6C)chloroalkyl, -(2-6C)chlorofluoroalkyl, -(2-6C)difluorochloroalkyl, -(2 6C)chlorohydroxyalkyl, -(1-6C)hydroxyalkyl, -(2-6C)dihydroxyalkyl, -(1-6C alkyl)CN, 0 (1-6C alkyl)SO

2 NH 2 , -(1-6C alkyl)NHSO 2 (1-3C alkyl), -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl), -(1-6C alkyl)N(1-4C alkyl) 2 , -(1-6C alkyl)NHC(=0)O(1-4C alkyl), (1-6C alkyl)hetCyc', -(1-6C alkyl)hetArl, hetAr 2 , hetCyc 2 , -O(1-6C alkyl) which is optionally substituted with halogen, OH or (1-4C)alkoxy, -O(3-6C cycloalkyl), Cyc', -(1-6C alkyl)(3-6C cycloalkyl), -(1-6Calkyl)(1-4C alkoxy), -(1-6C hydroxyalkyl)(1-4C alkoxy), a 5 bridged 7-membered cycloalkyl ring optionally substituted with (1-6C)hydroxyalkyl, or a bridged 7-8 membered heterocyclic ring having 1-2 ring nitrogen atoms; or NR R 2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from (1-6C)alkyl, OH, CO 2 H, (1-3C alkyl)CO 2 H, -0(1 -6C alkyl) and (1 -6C)hydroxyalkyl; 20 hetCyc 1 is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc' is optionally substituted with oxo, OH, halogen or (1-6C)alkyl; hetCyc 2 is a 6 membered carbon-linked heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc 2 is optionally substituted with F, 25 SO 2 NH 2 , SO 2 (1-3C alkyl) or halogen; hetArl is a 5-membered heteroaryl ring having 1-2 ring heteroatoms independently selected from N and 0 and optionally substituted with (1-4C)alkyl; hetAr 2 is a 5-6 membered heteroaryl ring having 1-2 ring nitrogen atoms and optionally substituted with one or more substituents independently selected from (1-4C)alkyl, 30 (3-6C)cycloalkyl, halogen and OH; WO 2011/006074 PCT/US2010/041538 171 Cyc' is a 3-6 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H, -(1-4C alkyl)OH, halogen and CF

3 ; Y is (i) phenyl optionally substituted with one or more substituents independently 5 selected from halogen, (1-4C)alkoxy, -CF 3 -CHF 2 , -O(1-4C alkyl)hetCyc 3 , -(1-4C alkyl)hetCyc 3 , -O(1-4C alkyl)O(1-3C alkyl) and -O(3-6C dihydroxyalkyl), or (ii) a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl), (1-4C)alkyl and NH 2 , or (iii) a pyrid-2-on-3-yl ring 0 optionally substituted with one or more substituents independently selected from halogen and (1-4C)alkyl; hetCyc 3 is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0 and optionally substituted with (1-6C)alkyl; X is -CH 2 -, -CH 2 CH 2 -, -CH 2 0- or -CH 2 NR d_; 5 Rd is H or -(1-4C alkyl); R3 is H or -(1-4C alkyl); each R 4 is independently selected from halogen, -(1-4C)alkyl, -OH, -(1-4C)alkoxy, NH 2 , -NH(1-4C alkyl) and -CH 2 OH; and n is 0, 1, 2, 3, 4, 5 or 6. 0 2. A compound of claim 1, wherein: R 1 is H or -(1-6C alkyl); R2 is H, -(1-6C)alkyl, -(1-6C)fluoroalkyl, -(1-6C)hydroxyalkyl, -(2 6C)dihydroxyalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2 NH 2 , -(1-6C alkyl)NHSO 2 (1-3C alkyl), -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl), -(1-6C alkyl)N(1-4C alkyl) 2 , -(1 25 6C alkyl)hetCyc', -(1-6C alkyl)hetArl, hetAr 2 , hetCyc 2 , -O(1-6C alkyl), -O(3-6C cycloalkyl), Cyc', or a bridged 7-membered cycloalkyl ring, or NR R 2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from -(1-6C)alkyl, -OH, -CO 2 H and -(1-3C alkyl)CO 2 H; 30 hetCyc' is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc' is optionally substituted with oxo; WO 2011/006074 PCT/US2010/041538 172 hetCyc 2 is a 6 membered carbon-linked heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0, wherein hetCyc 2 is optionally substituted with F, SO 2 NH 2 , or SO 2 (1-3C alkyl); hetArl is a 5-membered heteroaryl ring having 1-2 ring heteroatoms independently 5 selected from N and 0 and optionally substituted with -(1-4C)alkyl; hetAr 2 is a 5-6 membered heteroaryl ring having 1-2 ring nitrogen atoms and optionally substituted with one or more substituents independently selected from -(1 4C)alkyl; Cyc' is 3-6 membered cycloalkyl ring which is optionally substituted with one or 0 more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H and -(1-4C alkyl)OH; Y is (i) phenyl optionally substituted with one or more substituents independently selected from halogen, -(1-4C)alkoxy, -CF 3 -CHF 2 , -O(1-4C alkyl)hetCyc 3 and -O(1-4C alkyl)O(1-3C alkyl), or (ii) a 5-6 membered heteroaryl ring having a ring heteroatom selected 5 from N and S, wherein said heteroaryl ring is optionally substituted with one or more substituents independently selected from halogen, -O(1-4C alkyl) and (1-4C)alkyl; hetCyc 3 is a 5-6 membered heterocyclic ring having 1-2 ring heteroatoms independently selected from N and 0; X is -CH 2 -, -CH 2 CH 2 -, -CH 2 0- or -CH 2 NRd-; 0 Rd is H or -(1-4C alkyl); R3 is H or -(1-4C alkyl); each R4 is independently selected from halogen, -(1-4C)alkyl, -OH, -(1-4C)alkoxy, NH 2 , NH(1-4C alkyl) and CH 2 OH; and n is 0, 1, 2, 3, 4, 5 or 6. 25 3. A compound according to claims 1 or 2, wherein: R 1 is H or -(1-6C alkyl); and R2 is H, -(1-6C)alkyl, -(1-6C)fluoroalkyl, -(1-6C)hydroxyalkyl, -(2 6C)dihydroxyalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2 NH 2 , -(1-6C alkyl)NHSO 2 (1-3C alkyl), -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl), -(1-6C alkyl)N(1-4C alkyl) 2 , -(1 30 6C alkyl)hetCyc', -(1-6C alkyl)hetArl, hetAr 2 , -O(1-6C alkyl), -O(3-6C cycloalkyl), or a 3, 4 or 5 membered cycloalkyl ring optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H and -(1-4C alkyl)OH, WO 2011/006074 PCT/US2010/041538 173 or NR R 2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from -(1-6C)alkyl, -OH, -CO 2 H and -(1-3C alkyl)CO 2 H.

4. A compound according to claims 1 or 2, wherein: 5 R 1 is H or -(1-6C alkyl); R2 is H, -(1-6C)alkyl, -(1-6C)fluoroalkyl, -(1-6C)hydroxyalkyl, -(2 6C)dihydroxyalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2 NH 2 , -(1-6C alkyl)NHSO 2 (1-3C alkyl), -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl), -(1-6C alkyl)N(1-4C alkyl) 2 , -(1 6C alkyl)hetCyc', -(1-6C alkyl)hetArl, hetAr 2 , hetCyc 2 , -O(1-6C alkyl), -O(3-6C cycloalkyl), 0 or a bridged 7-membered cycloalkyl ring, or NR 1 R 2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from -(1-6C)alkyl, -OH, -CO 2 H and -(1-3C alkyl)CO 2 H.

5. A compound according to any of claims 1-4, wherein R2 is H or -(1-6C)alkyl. 5

6. A compound of claim 5, wherein R2 is -(1-6C)alkyl.

7. A compound of claim 6, wherein R2 is methyl, ethyl, isopropyl or tert-butyl.

8. A compound of claim 5, wherein R2 is H.

9. A compound according to any of claims 1-4, wherein R2 is -(1 6C)hydroxyalkyl or -(2-6C)dihydroxyalkyl. 0

10. A compound of claim 9, wherein R 2 is CH 2 CH 2 OH, CH 2 CH(OH)CH 2 OH or C(CH 3 )(CH 2 OH) 2 .

11. A compound of claim 1 or 2, wherein R 2 is Cyc' or a bridged 7-membered cycloalkyl ring.

12. A compound according to claim 11, wherein R 2 is CycI optionally substituted 25 with optionally substituted with one or two substituents independently selected from methyl, -OH, -CH 2 OH and -CO 2 H.

13. A compound according to claim 12, wherein R 2 is cyclopropyl.

14. A compound of claim 1 or 2, wherein Cyc' is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected 30 from -(1-4C alkyl), -OH, -OMe, -CO 2 H and -(1-4C alkyl)OH.

15. A compound of claim 1, wherein Cyc' is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from (1-4C alkyl), -OH, -OMe, -CO 2 H, -(1-4C alkyl)OH, halogen and CF 3 . WO 2011/006074 PCT/US2010/041538 174

16. A compound according to claim 1 or 2, wherein R 2 is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H and -(1-4C alkyl)OH.

17. A compound according to claim 16, wherein R 2 is cyclopropyl optionally 5 substituted with one or more substituents independently selected from methyl, -CO 2 H, or -CH 2 OH.

18. A compound of claim 1 or 2, wherein R2 is -O(1-6C alkyl) or -O(3-6C cycloalkyl).

19. A compound of claim 18, wherein R 2 is selected from -OMe, -OEt and 0 cyclopropoxy.

20. A compound of claim 1 or 2, wherein R 2 is selected from -(1-6C)fluoroalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2 NH 2 , and -(1-6C alkyl)NHSO 2 (1-3C alkyl).

21. A compound of claim 20, wherein R 2 is selected from -C(CH 3 ) 2 CH 2 F, -C(CH 3 ) 2 CH 2 OH, CH 2 C(CH 3 ) 2 OH, -CH 2 CN, -C(CH 3 ) 2 CN, -CH 2 CH 2 SO 2 NH 2 , 5 -CH 2 CH 2 NHSO 2 CH 3 and -C(CH 3 ) 2 CH 2 NHSO 2 CH 3 .

22. A compound of claim 1 or 2, wherein R 2 is selected from -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl) and -(1-6C alkyl)N(1-4C alkyl) 2 .

23. A compound of claim 22, wherein R 2 is selected from -CH 2 C(CH 3 ) 2 NH 2 , -C(CH 3 ) 2 NHCH 3 and -(1-6C alkyl)NMe 2 . 0

24. A compound of claim 1 or 2, wherein R 2 is selected from -(1-6C alkyl)hetCyc' and -(1-6C alkyl)hetArl.

25. A compound of claim 1 or 2, wherein R 2 is selected from -(1-6C alkyl)hetArl and hetAr 2 .

26. A compound according to any of claims 1-25, wherein R 1 is H. 25

27 A compound of claim 1, wherein NR 1 R 2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from -(1 6C)alkyl, -OH, -CO 2 H and -(1-3C alkyl)CO 2 H.

28. A compound according to any of claims 1-27, wherein X is -CH 2 - or -CH 2 CH 2 -. 30

29. A compound of claim 28 wherein X is -CH 2 -.

30. A compound according to any of claims 1-27, wherein X is -CH 2 0-.

31. A compound according to any of claims 1-27, wherein X is -CH 2 NR-. WO 2011/006074 PCT/US2010/041538 175

32. A compound according to any of claims 1-31, wherein Y is phenyl optionally substituted with one or more substituents independently selected from halogen, -(1-4C)alkoxy, CF 3 , CHF 2 , -0(1-4C alkyl)hetCyc 3 , -(1-4C alkyl)hetCyc 3 and -0(1-4C alkyl)0(1-3C alkyl). 5

33. A compound of claim 32, wherein Y is phenyl optionally substituted with one or more substituents independently selected from -F, -Cl, -OMe, -CF 3 , -CHF 2 , morpholinylethoxy, morpholinylethyl and -OCH 2 CH 2 OMe.

34. A compound of claim 33, wherein Y is phenyl, 3-fluorophenyl, 2,5 difluorophenyl, 2-chloro-5-fluorophenyl, 2-methoxyphenyl, 2-methoxy-5-fluorophenyl, 2 0 trifluoromethyl-5-fluorophenyl, 2-difluoromethyl-5-fluorophenyl, 3-chloro-5-fluorophenyl, 3-fluoro-5-(2-morpholinylethoxy)phenyl, 3-fluoro-5-(2-morpholinylethyl)phenyl, 5-fluoro 2-(2-morpholinylethyl)phenyl, 3-fluoro-5-methoxyethoxyphenyl or 5-fluoro-2 methoxyethoxyphenyl.

35. A compound according to any of claims 1-31, wherein Y is fluorophenyl 5 optionally substituted with a substituent selected from -0(1-4C alkyl)hetCyc 3 , -0(1-4C alkyl)0(1-3C alkyl) and -0(3-6C dihydroxyalkyl).

36. A compound of claim 35, wherein Y is fluorophenyl substituted with a substituent selected from morpholinylethoxy, -OCH 2 CH 2 OMe, 2,3-dihydroxypropoxy and 2,2-dimethyl-1,3-dioxolanyl. 0

37. A compound of claim 34, wherein Y is 2,5-difluorophenyl.

38. A compound according to any of claims 1-31, wherein Y is a 5-6 membered heteroaryl ring having a ring heteroatom selected from N and S, wherein said ring is optionally substituted with one or more substituents independently selected from halogen, -0(1-4C alkyl) and (1-4C)alkyl. 25

39. A compound of claim 38, wherein Y is pyridyl optionally substituted with one or more substituents independently selected from F, -OMe and Me.

40. A compound of claim 39, wherein Y is pyrid-2-yl, pyrid-3-yl, 5-fluoropyrid-3 yl, 2-methoxy-5-fluoropyridy-3-yl or 2-methyl-5-fluoropyridy-3-yl.

41. A compound according to any of claims 1-31, wherein Y is a 5-6 membered 30 heteroaryl ring having a ring heteroatom selected from N and S, wherein said ring is substituted with one or more substituents independently selected from halogen and (1 4C)alkyl. WO 2011/006074 PCT/US2010/041538 176

42. A compound of claim 41, wherein Y is pyridyl substituted with one or more substituents independently selected from F, methyl and ethyl.

43. A compound of claim 42, wherein Y is 5-fluoropyrid-3-yl, 2-methyl-5 fluoropyrid-3-yl or 2-ethyl-5-fluoropyrid-3-yl. 5

44. A compound of claim 40 or 43, wherein Y is 5-fluoropyrid-3-yl.

45. A compound according to any of claims 1-31, wherein Y is a pyrid-2-on-3-yl ring optionally substituted with one or more substituents independently selected form halogen and (1-4C)alkyl.

46. A compound of claim 45, wherein Y is 5-fluoropyridin-2(1H)-one optionally 0 substituted with (1-4C)alkyl.

47. A compound of claim 1, wherein: R 1 is H or -(1-6C alkyl); R2 is H, -(1-6C)alkyl, -(1-6C)fluoroalkyl, -(1-6C)hydroxyalkyl, -(2 6C)dihydroxyalkyl, -(1-6C alkyl)CN, -(1-6C alkyl)SO 2 NH 2 , -(1-6C alkyl)NHSO 2 (1-3C 5 alkyl), -(1-6C alkyl)NH 2 , -(1-6C alkyl)NH(1-4C alkyl), -(1-6C alkyl)N(1-4C alkyl) 2 , -(1 6C alkyl)hetCyc', -(1-6C alkyl)hetArl, hetAr 2 , hetCyc 2 , -O(1-6C alkyl), -O(3-6C cycloalkyl), or Cyc'; or NR R 2 forms a 4-6 membered azacyclic ring optionally substituted with one or more substituents independently selected from -(1-6C)alkyl, -OH, -CO 2 H and -(1-3C 0 alkyl)CO 2 H; Cyc' is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C alkyl), -OH, -OMe, -CO 2 H and -(1 4C alkyl)OH. X is CH 2 ; and 25 Y is (i) fluorophenyl optionally substituted with a substituent selected from -O(1-4C alkyl)hetCyc 3 , -(1-4C alkyl)hetCyc 3 , -O(1-4C alkyl)O(1-3C alkyl) and -O(3-6C dihydroxyalkyl), (ii) pyridyl substituted with one or more substituents independently selected from F, methyl and ethyl, or (iii) 5-fluoropyridin-2(1H)-one optionally substituted with (1 4C)alkyl. 30

48. A compound of claim 47, wherein Y is fluorophenyl optionally substituted with a substituent selected from -O(1-4C alkyl)hetCyc 3 , -(1-4C alkyl)hetCyc 3 , -O(1-4C alkyl)O(1-3C alkyl) and -0(3-6C dihydroxyalkyl). WO 2011/006074 PCT/US2010/041538 177

49. A compound of claim 47, wherein Y is pyridyl substituted with one or more substituents independently selected from F, methyl and ethyl.

50. A compound of claim 47, wherein Y is 5-fluoropyridin-2(1H)-one optionally substituted with (1-4C)alkyl. 5

51. A compound according to any of claims 47-50, wherein R2 is a 3, 4 or 5 membered cycloalkyl ring which is optionally substituted with one or more substituents independently selected from -(1-4C)alkyl, -OH, -OMe, -CH 2 OH and -(1-4C)alkylOH.

52. A compound according to claim 51, wherein R4 is OH, F, methyl, or CH 2 OH.

53. A compound according to claim 52, wherein n is 0, 1 or 2. 0

54. A compound according to claim 53, wherein R3 is hydrogen.

55. A compound according to any of claims 1-54, wherein Y has the absolute configuration of Figure Ia: f-N N R3N ' N N R1 (R4)n~, N/ x 0 R Ia 5

56. A compound according to any of claims 1-55, wherein R3 is H.

57. A compound according to any of claims 1-56, wherein n is 0-2 and R4 is F or Me.

58. A compound according to claim 57, wherein n is 0.

59. A pharmaceutical composition, which comprises a compound of Formula I as 20 defined in any one of claims 1 to 58, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable diluent or carrier.

60. A method for treating a disease or disorder selected from pain, cancer, inflammation, neurodegenerative disease or Trypanosoma cruzi infection in a mammal, which comprises administering to said mammal a therapeutically effective amount of a 25 compound of Formula I as defined in any one of claims I to 58, or a pharmaceutically acceptable salt thereof.

61. The method of claim 60, wherein the disease or disorder is pain.

62. A compound of Formula I as defined in any one of claims 1 to 58, or a pharmaceutically acceptable salt thereof, for use in the treatment of pain, cancer, 30 inflammation, neurodegenerative disease or Trypanosoma cruzi infection. WO 2011/006074 PCT/US2010/041538 178

63. A process for the preparation of a compound of claim 1, which comprises: (a) reacting a corresponding compound of Formula II R 3 0 'N YN N N (R 4 )n--- OH x o II 12 5 or a reactive derivative thereof with an amine having the formula HNR R ; or (b) for compounds of Formula I where R 1 and R 2 are each hydrogen, reacting a compound of Formula III R 3 >LN- N N (R 4 )n-- C N x III 0 with an inorganic acid; or (c) for a compound of Formula I where R 2 is (alkyl)NHSO 2 ((1-3C alkyl), reacting a compound having the formula IV R3 N N N (R4)- NH(1 -6C alkyl)NH 2 x o IV L 5 with (1-3C alkyl)SO 2 Cl; or (d) for compounds of Formula I wherein Y is 5-fluoropyridin-2(1H)-one, treating a corresponding compound having the formula VIII F N R3 N NN N N R1 X O R 2 (R 4) VIII 20 with an acid at elevated temperatures; or (e) for a compound of Formula I wherein R 2 is CH 2 CH(OH)CH 2 OH, treating a corresponding compound having the formula IX WO 2011/006074 PCT/US2010/041538 179 R3\ R 3 N -.. 7-N N R (R4)n---- N x o - O 0+ Ix with an acid; or (f) for a compound of Formula I wherein Y is fluorophenyl substituted with 5 OCH 2 CH(OH)CH 2 OH, treating a corresponding compound having the formula X F R3 N O- N R1 /2:-- N x with an acid; and removing or adding any protecting groups if desired, and forming a salt if desired. 0