AU2015201228B2 - Treatment for neoplastic diseases - Google Patents

Abstract

Administration of a mAb that specifically binds IL-la is useful for treating tumor-associated diseases in human subjects.

Description

TREATMENT FOR NEOPLASTIC DISEASES CROSS-REFERENCE TO RELATED APPLICATIONS [0001] The present application claims the priority of U.S. provisional patent application serial numbers 61/376,097 filed on August 23, 2010, 61/406,759 filed on October 26, 2010, 61/41 1,183 filed on November 8, 2010, and 61/480,635 filed on April 29, 201 1, all of which are incorporated herein by reference in their entirety. STATEMENT AS TO FEDERALLY SPONSORED RESEARCH [0002] Not applicable. FIELD OF THE INVENTION [0003] The invention relates generally to the fields of medicine, oncology, and immunology. More particularly, the invention relates to the use of antibodies (Abs) which specifically bind interleukin-la (IL-la) to treat a tumor-associated disease and other tumor-associated pathologies. BACKGROUND [0003a] Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common general knowledge in the field. [0004] Despite many advances, tumor-associated diseases such as cancer remain one of the leading causes of death and morbidity in developed nations. Although many of the molecular mechanisms of tumorigenesis have now been revealed, standard treatment of most aggressive tumors continues to be surgical resection, chemotherapy, and radiotherapy. While increasingly successful, each of these treatments still causes numerous undesired side effects. For example, surgery results in pain, traumatic injury to healthy tissue, and scarring. Radiotherapy and chemotherapy cause nausea, immune suppression, gastric ulceration, and secondary tumorigenesis. [0005] Over the last several years much progress has been made using biologic agents such as Abs to treat cancerous tumors. Abs can directly target specific types of tumor cells to harness a patient's immune response to kill the tumor. Alternatively, they can target cell growth factors to interfere with the growth of tumor cells. As with conventional chemotherapeutic agents, not all anti-tumor Abs are useful for treating all types of neoplasms and many initially - 1 effective antibodies later lose potency. Thus, new anti-tumor Abs are needed. SUMMARY [0005a] According to a first aspect, the present invention provides a method for decreasing the size of a cancerous tumor in a human subject having a cancer that has progressed after treatment with chemotherapy, radiotherapy, or biologic agents, the method comprising the step of administering to the subject a pharmaceutical composition comprising a pharmaceutically acceptable carrier and an amount of an anti-IL-1a antibody effective to reduce the size of the tumor in the subject. [0005b] According to a second aspect, the present invention provides use of an anti-IL-1a antibody in the preparation of a medicament for treating a cancer that has progressed after treatment with chemotherapy, radiotherapy, or biologic agents. [0005c] Unless the context clearly requires otherwise, throughout the description and the claims, the words "comprise", "comprising", and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense; that is to say, in the sense of "including, but not limited to". [0006] The invention is based on the discovery that a mAb that specifically binds IL-la is useful for treating various tumor-associated diseases. - la - [00071 Accordingly, the invention features a niedicanent and method for treating neoplastic diseases (e~g., a colorectal cancer such as one having a KRAS mutation, an EBV-associated cancer such as nasopharygeal carcinoma or Burkitt's lymphoma, non-small cell lung cancer (NSCIC) or non-cancerous conditions associated with tumors such as Castleman's disease) in a hmnan subject The method can be performed by administering to the subject a pharmaceutical composition including a pharmaceutically acceptable carrier and an amount of an anti-IL-la Ab effective to ameliorate a symptom of a tumor-associated pathology and/or to reduce the size of a tumor in the subject by at least about 10% (e.g, at least 8, 9, 10, 15, 17, 20, 30, 40, 50, 60, 70, 80, 90, or 100%). The medicament can include an anti-IL-I o. Ab. The anti-IL-l( Ab can be a mAb such as an IgGi. The antiIL--l Ab can be the mA.b designated as MABpl or a mAb that inchides one or more complementanty determining regions (CD Rs) of MABp I. [0008] The pharmaceutical composition can be administered to the subject by injection, subcutaneously, intravenously, intramuscularly., or directly into a tumor. In the method, the dose can be at least 0.25 (e.g, at least 0.2, 0.5, 0.75., 1, 2, 3, 4, or 5) mg/ml [00091 Unless otherwise defined, all technical terms used herein have the same meaning as commonly iderstood by one of ordinary skill in the art to which this invention belongs. Commonly understood definitions of biological terms can be fond in Rieger et al, Glossary of Genetics: Classical and Molecular, Sth edition, Springer Verlag: New York, 1991; and Lewin, Genes V, Oxford University Press: New York, 1994. Commonly understood definitions of medical terms can be found in Stedman's Medical Dictionary, 27t Edition, Lippincott, Williams & Wilkins, 2000. [00-10 As used herein, an "Ab" or "Ab" is an immunoglobulin (1g), a solution of identical or heterogeneous Igs, or a mixture of Igs.An "Ab" can also refer to fragments and engineered versions of Igs such as Fat, Fab, and [(ab')2 fragments; and scFv's, heteroconjugate Abs, and similar artificial molecules that employ 1g derived CDRs to impart antigen specificity, A "mAb" or "mAb" is an Ab expressed by one clonal B cell line or a population of Ab molecules that contains only one species of an antigen binding site capable of immunoreacting with a particular epitope of a particular antigen. A "polyclonal Ab" or "polyclonal Ab" is a mixture of heterogeneous Abs. Typically, a polyclonal Ab will inchide myriad different Ab 2 molecules which bind a particular antigen with at least some of the different Abs immunoreacting with a different epitope of the antigen, As used herein, a polyclonal Ab can be a mixture of two or more mAbs. 100111 An. "antigen-binding portion" of an Ab is contained within the variable region of the Fab portion of an Ab and is the portion of the Ab that confers antigen specificity to the Ab (he typically the three-dimensional pocket formed by the CDRs of the heavy and light chains of the Ab). A "Fab portion" or "Fab region" is the proteolytic fragment of a papain-digested Ig that contains the antigen-binding portion of that Ig. A "non-Fab portion" is that portion of an Ab not within the Fab portion, e.g., an "Fe portion" or "Fe region." A "constant region" of an Ab is that portion of the Ab outside of the variable region. Generally encompassed within the constant region is the "effector portion" of an Ab, which is the portion of an Ab that is responsible for binding other immune system components that facilitate the immune response. This, for example, the site on an Ab that binds complement components or Fc receptor; (not via its antigen-binding portion) is an effector portion of that Ab, 100121 When referring to a protein molecule such as an Ab, "purified" means separated from components that naturally accompany such molecules, Typically, an Ab or protein is punfied when it is at least about 1I,% (eg, 9%, 10%, 20%, 30% 40%, 50%, 60%, 70%, 80%, 90%, 95%, 98%, 99%, 99.9%, and 100%), by weight, free from the non-Ab proteins or other naturally-occurring organic molecules with which it is naturally associated. Purity can be measured by any appropriate method, e.g., column chromatography, polyacrylamide gel electrophoresis, or HPLC analyst is. A chemically-synthesized protein or other recombinant protein produced in a cell type other than the cell type in which it naturally occurs is "purified." [001.3] By "bind", "binds". or "reacts with" is meant that one molecule recognizes and adheres to a particular second molecule in a sample, but does not substantially recognize or adhere to other molecules in the sample. Generally, an Ab that "specifically binds" another molecule has a Kj greater than about 105 l0, 10, 10', 10, 10" ,I 10", or 10"- liters/mole for that other molecule. 10014] A "therapeutically eflective amount" is an. amount which is capable of producing a medically desirable effect in a treated animal or human (e.g, amelioration or prevention of a disease or symptom of a disease). [0015] Although methods and materials similar or equivalcut to those described herein can be used in the practice or testing of the present invention, suitable methods 3 and Materials are described below, All publications mentioned herein are incorporated by reference in their entirety. In the case of conflict, the present specification, including definitions will control. In addition, the particular embodiments discussed below are illustrative only and not intended to be limiting. DETAILED DESCRIPTION [001,61 The invention encompasses compositions and methods for ameliorating one or more symptoms of a tumor-associated pathology in a subject. The below described preferred embodiments illustrate adaptation of these compositions and methods. Nonetheless, from the description of these embodiments, other aspects of the invention can be made and/or practiced based on the description provided below. General Methodology 1001.71 Methods involving conventional imnnological and molecular biological are~-i described heen Imunogicaca techniques are described herein, Immunological methods (for example., assays for detection and localization of antigen-Ab complexes, immunoprecipitation, immunoblotting, and the like) are generally known in the art and described in methodology treatises such as Current Protocols in inmmunology, Coligan et at. ed., John Wiley & Sons, New York. Techniques of molecular biology are described in detail in treatises such as Molecular Cloning: A Laboratory Manual, 2nd ed., vol. 1-3, Sambrook et al, ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N:Y., 2001; and Current Protocols in Molecular Biology, Ausubel et al., ed., Greene Publishing and Wiley-Interscience, New York. Ab methods are described in Handbook of Therapeutic Abs, Dubel, S., ed., Wiley-VCR, 2007. General methods of medical treatment are described in McPhee and Papadakis, Current Medical Diagnosis and Treatment 2010., 49"' Edition, McGraw-Hill Medical, 2010; and Fauci et al., Harrison's Principles of Internal Medicine, 17d Edition, McGraw-Hill Professional, 2008 Treatment of a Tumor-associated Disease [0018] The compositions and methods described herein are useful for treating a tumor-associated disease in a mammalian subject by administering to the subject a pharmaceutical composition including an amount of an anti-IL-a I Ab effective to improve at least one characteristic of the tumor-associated disease in the subject. The mammalian subject might be any that suffers from a tumor-associated disease including, human beings, dogs, cats, horses, cattle, sheep, goats, and pigs. Human 4 subjects might be male, female, adults, children, seniors (65 and older), and those with other diseases. Particularly preferred subjects are those whose disease has progressed after treatment with chemotherapy, radiotherapy, surgery, and/or biologic agents. Any type of a tumor-associated disease susceptible to treatment with an anti IL-la Ab might be targeted. Anti-L-1a Ab administration is thought to be particularly effective for treating colorectal tumors (e0g, colorectal cancers with a KRAS mutation), EBV-associated neoplasms such as nasopharyngeal cancer or Burkitt's lymphoma, NSCLC, and blood cell neoplasms such as in Castleman's disease. A disease with tumor. expressing IL-la or tenors infiltrated with IL I (I inflammatory cells might also be targeted, The particular characteristic of a tumor-associated disease to be improved can be tumor size (e.g., TO, Tis, or TI-4), state of metastasis (e.g., MO, M.l), number of observable tumors, node involvement (e.g., NO, N1-4, Nx), grade (i.e., grades 1, 2, 3, or 4), stage (e.g, 0,1 1, 111, or IV), presence or concentration of certain markers on the cells or in bodily fluids (e.g., AFP, 12M, beta-HCG, BTA, CA 15-3. CA 27.29, CA 125, CA 72.4, CA 19-9, calcitonin, CEA., chromgrainin A, EGFR, hormone receptors, H-ER2, HCO, immunoglobulins, NSE, NM% P22, PSA, PAP, PSMA, S-100, TA-90, and thyroglobulin), and/or associated pathologies (e.g., ascites or edema) or symptoms (e.g, cachexia, fever, anorexia, or pain). The improvement, if measureable by percent, can be at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 70, 80, or 90% (e.g., volume or linear dimensions of a tumor). Antibodies and other Agents that Target IL-la [0019] Any suitable type of Ab or other biologic agent (e.g,., a fusion protein including an IL-Ia-binding component such as an IL-I receptor) that specifically binds IL-l a and reduces a characteristic of a tumor-associated disease in a subject might be used in the invention. For example, the anti-IL-I a Ab used might be mAb, a polyclonal Ab, a mixture of mAbs, or an Ab fragment or engineered Ab-like molecule such as an scFv The Ka of the Ab is preferably at least I xl0V M" or greater (e.g., greater than 9 x10 M , 8 0 xl0" M V. 7 x.0"0 6 x.' M 5 x10" M', 4 x10" N, 3 x10 O 2 x 10 M or I xlO 0

N

1 ). In a preferred embodiment, the invention utilizes a. fully human mAb that includes (i) an antigen binding variable region that exhibits very high binding affinity (e.g, at least nano or picomolar) for human IL-la and (ii) a constant region. The human Ab is preferably

S

an IgGI, ahhough it might be of a different isotype such as 1gM, .gA, or IgE, or subclass such as lgG2, IgG3, or igG4, One example of a particularly useful mAb is MABpI, an IL-la-specific IgG1 mAb described in US. patent application serial number 12/455,458 filed on June 1, 2009. Other useful mAbs are those that include at least one but preferably all the CDRs of MABpIL [00201 Because B lymphocytes which express ig specific for human ILI a occur naturally in human beings, a presently preferred method for raising mAbs is to first isolate such a B lymphocyte from a subject and then immortalize it so that it can be continuously replicated in culture, Subjects lacking large numbers of naturally occurring B lymphocytes which express Ig specific for human IL-la may be immunized with one or more human IL- I a antigens to increase the number of such B lymphocytes. Human mAbs are prepared by immortalizing a human Ab secreting cell (eg, a human plasma cell). See, e.g., U.S. patent no. 4,634,664, [00211 in an exemplary method, one or more (e.g, 5, 10, 25, 50, 100, 1000, or more) human subjects are screened for the presence of such human IL- 1 a-specific Ab in their blood. Those subjects that express the desired Ab can then be used as B lymphocyte donors. In one possible method, peripheral blood is obtained from a human donor that possesses B lymphocytes that express human IL-la-specific Ab. Such B lymphocytes are then isolated from the blood sample, e.g. by cells sorting (e.g, fluorescence activated cell sorting, "FACS"; or magnetic bead cell sorting) to select B lymphocytes expressing human IL-la-specific Ig. These cells can then be immortalized by viral transformation (e.g., using EBV) or by fusion to another innortalized cell such as a human myeloma according to known techniques. The B lymphocytes within this population that express Ig specific for human IL-la can then be isolated by limiting dilution tnethods (e.g, cells in wells of a microtiter plate that are positive for Ig specific for human IL-la are selected and subcuhured, and the process repeated until a desired clonal line can be isolated). See, e.g., Goding, MAbs: Principles and Practice, pp. 59-103, Academic Press, 1986. Those clonal cell lines that express Ig having at least nanomolar or picomolar binding affinities for human IL-la are preferred, MAbs secreted by these clonal cell lines can be purified from the culture medium or a bodily fluid (e.g, ascites) by conventional Ig purification procedures such as salt cuts, size exclusion, ion exchange separation, and affinity chromatorgraphy. 6 [0022] Although immortalized B lymphocytes might be used in in vitro cultures to directly produce nmAbs, in certain cases it might be desirable to use heterologous expression systems to produce mAbs. See, e.g., the methods described in U.S. patent application number 11/754.899. For example, the genes encoding an mAb specific for human IL-la might be cloned and introduced into an expression vector (e,g., a plasmid-based expression vector) for expression in a heterologous host cell (eg, CHO cell, COS cells, mycloma cells, and E. col cells). Because Igs include heavy (H) and light (L) chains in an it 2 configuration, the genes encoding each may be separately isolated and expressed in different vectors. [0023] Although generally less preferred due to the greater likelihood that a. subject will develop an anti-Ab response, chimeric mAbs (e.g, "humanized" mAbs), which are antigen-binding molecules having different portions derived from different animal species (e.g., variable region of a mouse Ig fused to the constant region of a human Ig), might be used in the invention. Such chimeric Abs can be prepared by methods known in the art, See, e.g., Morrison et aL Proc. Nat'l Acad. Sci. USA, 81:6851, 1984; Neuberger et at, Nature, 312:604, 1984; Takeda et al, Nature, 314:452, 1984. Similarly, Abs can be humanized by methods known in the art. For example. nAbs with a desired binding specificity can be humanized by various vendors or as described in U.S. Pat. Nos. 5,693,762; 5,530,101; or 5,585,089. 100241 The niAbs described herein might be affinity matured to enhance or otherwise alter their binding specificity by known methods such as VH and VL domain shuffling (Marks et alt Bio/Technology 10:779-783, 1992), random mutagenesis of the hypervariable regions (IVRs) and/or framework residues (Barbas et at Proc Nat Acad. Sci. USA 91:3809-3813, 1994; Schieret al. Gene 169:1.47-155, 1995; Yelton ct al. J, Immunol. 155:1994-2004, 1995; Jackson et al., Y, Immunol. 154(7):3310-9, 1995; and Hawkins et al, J, Mot Biol. 226:889-896, 1992. Amino acid sequence variants of an Ab may be prepared by introducing appropriate changes into the nucleotide sequence encoding the Ab. In addition, modifications to nucleic acid sequences encoding mAbs might be altered (e.g., without changing the amino acid sequence of the mAb) for enhancing production of the mAb in certain expression systems (e.g, intron elimination and/or codon optimization for a given expression system), The mAbs described herein can also be modified by conjugation to another protein (e.g., another mAb) or non-protein molecule. For example, a mAb might be 7 conjugated to a water soluble polymer such as polyethylene glycol or a carbon nanotube (See, e.g, Kam et al, Proc. Nat], Acad. Sci, USA 102: 11600-11605, 2005). See, U.S. patent application number 11 /754,899. [0025] Preferably, to ensure that high titers of human i14u -specific mAb can be administered to a subject with minimal adverse effects, the mAb compositions of the invention are at least 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 95, 96, 97, 98, 99, 99.9 or more percent by weight pure (excluding any excipients). The mAb compositions of the invention might include only a single type of mAb (i.e., one produced from a single clonal B lymphocyte line) or might include a mixture of two or more (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10 or more) different types of mAbs. [0026] To modify or enhance their function, the human IL-hi mAbs might be conjugated with another molecule such as a. cytotoxin. A human IL-la specific mAb might be conjugated with one or more cytotoxins to more effectively kill cells expressing IL-la. Cytotoxins for use in the invention can be any cytotoxic agent (e.g., molecule that can kill a cell after contacting the cell) that can be conjugated to a human IL-la specific mAb, Examples of cytotoxins include, without limitation, radionuclides (e., S, NC P, .I, I 11y, 11Zr, - T1, 1 Re," ae, -Cu, " -Bi, and 21 'At), conjugated radionuclides, and chemotherapeutic agents. Further examples of cytotoxins include, but are not limited to, antimetabolites (e~g., 5-fluorouricil (5 FU), niethotrexate (MTXI, fludarabine, etc.), anti-microtubule agents (e.g, vincristine, vinblastine, colchicine, taxanes (such as paclitaxel and docetaxel), etc.), alkylating agents (e.g, cyclophasphamide, melphalan, biscloroethylnitrosurea (BCNU), etc.), platinum agents (e.g, cisplatin (also termed c:DDP), carboplatin, oxaliplatin, JM-216, CI-973, etc.), anthracyclines (e.g., doxorubicin, daunorubicin, etc.), antibiotic agents (e.g., mitomycin-C), topoisomerase inhibitors (e.g, etoposide, tenoposide, and camptothecins), or other cytotoxic agents such as ricin, diphtheria toxin (DT), Pseudoimonas exotoxin (PE) A, PE40, abrin, saporin, pokeweed viral protein, ethidium bromide, glucocorticoid, anthrax toxin and others. See, e.g., U.S. Pat. No. 5,932,188. 100271 While the IL-la specific Abs described above are preferred for use in the invention, in some cases, other agents that specifically target IL-la might be used so long as their administration leads to improvement of a characteristic of a tumor 8 associated disease. These other agents might include small organic molecules, aptaners, peptides, and proteins that specifically bind IL-la (e,g anakinra or rilonacept). Pharmaceutical Compositions and Methods [0028j The anti-IL-Ia( Ab compositions may be administered to animals or humans in pharmaceutically acceptable carriers (e.g, sterile saline), that are selected on the basis of mode and route of administration and standard pharmaceutical practice. A list of pharmaceutically acceptable carriers, as well as pharmaceutical formulations, can be found in Remington's Pharmaceutical Sciences, a standard text in this field, and in USP/NFP. Other substances may be added to the compositions and other steps taken to stabilize and/or preserve the compositions, and/or to facilitate their administration to a subject. 100291 For example, the Ab compositions might be lyophilized (see Draber et al, . immunol. Methods, 181:37, 1995; and PCT/US90/01383); dissolved in a solution including sodium and chloride ions; dissolved in a solution including one or more stabilizing agents such as albumin, glucose, maltose, sucrose, sorbitol, polyethylene glycol, and glycine; filtered (e.g., using a 0.45 and/or 0.2 micron filter); contacted with beta-propiolactone; and/or dissolved in a solution including a microbicide (e.g, a detergent, an organic solvent, and a mixture of a detergent and organic solvent. 100301 The Ab compositions may be administered to animals or humans by any suitable technique. Typically, such administration will be parenteral (e,g., intravenous, subcutaneous, intramuscular, or intraperitoneal introduction), The compositions may also be administered directly to the target site (e.g., intratumorally) by, for example, injection. Other methods of delivery, e.g, liposomal delivery or diffusion from a device impregnated with the composition, are known in the art. The composition may be administered in a single bolus, multiple injections, or by continuous infusion (e.g., intravenously or by peritoneal dialysis) 100311 A therapeutically effective amount is an amount which is capable of producing a medically desirable result in a treated animal or human. An effective amount of anti-IL-la Ab compositions is an amount which shows clinical efficacy in patients as measured by the improvement in one or more a tumor-associated disease characteristics described above. As is well known in the medical arts, dosage for any one animal or human depends on many factors, including the subject's size, body 9 surface area, age, the particular composition to be administered, sex, time and route of administration, general health, and other drugs being administered concurrently. Preferred doses range from about 0.2 to 20 (e.g,, 0.15, 0.2, 0.3, 0.4, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 30, 50, or 100) mg/kg body weight The dose may be given repeatedly, e.g.. hourly, daily, semi-weekly, weekly, bi-weekly, tri-weekly, or monthly. EXAMPLES Example I - Xilonixll" 100321 XilonixTM is a sterile injectable liquid formulation of 15 mg/mL MABpl in a stabilizing isotonic buffer (pH 6.4) Each 10-mL Type 1 borosilicate glass serum vial contains 5 mL of the formulation, and is sealed with a 20-mm Daikyo Flurotec butyl rubber stopper and flip-off aluminum seal The product is stored at 5+34C, with excursions to room temperature permitted, The exact composition of the drug product is shown below: Composition of the Drug Product (xilonix ... ingredient Grade Manufacturer Concentration MABpI Ab GMP XBiotech 15 mg/mL sodium phosphate dibasic compendial JT Baker 12 mgn/mL citric acid monohydrate compendial JT Baker 2 mg/mL Trehalose.2-120 (high-purity low compendial Ferro- 60 mgnmL endotoxin) Pfanstiehl polysorbate 80 compendial JT Baker 0.2 mg/ml, Phosphoric acid, to adj ust pH compendial JT Baker 0.04 mg/mL water for injection compendial Microbix qs, Method of Administration: 100331 The calculated volume is withdrawn from the drug (mAb)-containing vial(s) using a suitable syringe. The drug is then injected into a small W bag containing 100 mL of normal saline (0.9% NaCl) and mixed by inversion, The diluted drug product can be stored at room temperature for 3 hours prior to administration and is infused over a 1-hour period, with the subject being monitored for signs of an 10 infusion reaction. The infusion is chased with a minimum of 30 mLs of normal saline to deliver any product that may be held up in the infusion set, Example 2 - Treatment of Colorectal Cancer with an IL-I u-specific MAb (XilonixT'M). 100341 The human subject was a 63 year-old female diagnosed with metastatic colorectal cancer (KRAS mutation positive). Prior to treatment with XilonixM. the subject underwent right hemicolectomy and was reportedly staged T3N.1MX, She thereafter received adjuvant chemotherapy with FOLFOX for a total of 12 cycles over about six months, A PET CT scan performed about two months after completion of FOLFOX revealed a mass in her pelvis. The subject was hospitalized at the time for placement of areteral stent due to obstructive hydronephrosis apparently from the tumor, She started FOLFIRI and Avastin shortly thereafter and received 8 cycles of therapy. The subject then underwent re-staging PET CT scan which confirmed disease in the pelvis, and also revealed small pulmonary nodiles consistent with metastatic disease. A CT scan of the chest, abdomen and pelvis revealed a 12 cm pelvic mass, a .2 cm mental mass, and the hydronephrosis on the right side with. associated ureteral sten. She received 2 extra cycles of FOLFIRI and Avastin. A subsequent PET CT scan showed progression of the bilateral pulmonary nodules. The subject then started irinotecan and Erbitux* (Cetuxinab) therapy. A follow up PET CT scan demonstrated disease progression in the lungs. [00351 The subject was initiated on a phase I trial with Doxil (Doxorubicin Liposomal), Velcade* (Bortezomib), and Ge.mzar* (G emcitabine) but unfortunately the first re-staging suggested disease progression. She also completed another phase 1 trial with oxaliplatin in combination with azacitydineon and completed 2 cycles before disease progression. At the conclusion of her participation on this last clinical phase 1 trial, the subject was enrolled in the current clinical trial. 100361 She was enrolled in the first dosing cohort (0.25 mg/m) and completed 5 -21 day cycles on the protocol, thus receiving a total of five infusions of MABpl (0.25 mg/kg) every 21 days. The subject's dose was increased to 075 mg/kg on Cycle 6 Day L, An initial PET CT scan revealed about a 17% reduction in the sum of diameters in the patient's tumors that were being tracked. Following additional doses of MABpI, an over 30% reduction in the sum of diameters in. the patient's 11.

tracked tumors was observed. A Chest CT showed a paratrocheal lymph node that previously measured 3.5 cm was reduced to 2.9 cm at the end of Cycle 6. A left lung metastasis decreased from 2.2 cm to 1.9 cm, and an implant from the left rectus muscle decreased from 3.2 cm to 2.7 cm. The CEA tumor marker at baseline was 81, decreased to 69,2 at the end of cycle 3, and was 27.9 as of Cycle 7 Dayl. This patient has continued on therapy for over 71 weeks and the disease has remained stable, Example 3 - Treatment of Nasopharygeal Cancer with an IL- a-specific MAb (Xilonix"m). 100371 The subject was a 47 year old Chinese male having EBV+ (Epstein-Barr virus) nasopharyngeal carcinoma with the histological subtype lymphoepithelioma (old terminology) or non-keratinizing carcinoma, The subject was previously treated with cisplatin, 5-FU, radiotherapy, Taxotere- (Docetaxel), Gemzar@ (Gemcitabine) , Xelodat (Capecitabi-ne), adoptive EBV-directed T cell transfer, and Cymevene"a Ganciclovir) in combination with Gemzarlk (Gemcitabine) . Prior to starting therapy, the patient had fatigue, fevers and sweats, and was receiving frequent therapeutic paracentesis for ascites. 100381 The subject began MABpl treatment on day 0 at 1.25 mg/kg IV every two weeks. By days 3 and 4, a marked decrease in the subjects fatigue, fevers, and sweats was noted. The ascites also resolved. Abdominal CT abdomen scans showed a reduction in the size of a metastatic liver tumor from 50,4mm on day I to 35.8mm by day 36 (almost 30%) of one of tie masses. Multiple other liver lesions decreased in size, and bone lesions appeared to be stable. Example 4 - Treatment of Castleman's Syndrome with an IL-ia-specific MAb (Xilonixf). [00391 The subject was a 55-year-old woman suffering from Castleman's disease (the variant known as POEMS syndrome). Her symptoms included fatigue, edema, and nerve pain. Prior treatment with Rituxan"' (Rituximab) and an investigational anti-iL-6 therapy failed. The subject was administered a total of four infusions of MABp. (0.75 mg/kg) every 21 days. The subject's dose was increased to 1.25 mg/kg in the next cycle, 12 [0040] This subject had stable disease through 2 re-stagings, and has been treated for over 4 months, For approximately 2 weeks after each injection, her symptoms of fatigue, edema, and nerve pain improved significantly, and then gradually recurred until the next injection. Her RECIST staging criteria showed 2% increase of lymph node size from baseline at the first restage, and 4% increase of lymph node size from baseline at the second restage. [0041] After completing 7 cycles, the subject withdrew consent for therapy in order to try another experimental treatment, Afler being off study for 8 weeks, the subject physician requested that she be allowed to resume therapy with M.ABp.1 due to "rapid disease progression". Since resuming treatment, the subject's disease is stable and she has been on study for over 58 weeks. Example 5 - Treatment of NSCLC with an IL-I a-specific MAb (XilonixmV) [00421 The subject was an 84 year old female with a history of metastatic non-small cell lung cancer diagnosed by fine needle aspiration. Three months after diagnosis, the subject began treatment with Tarcevak (Erlotinib) for 8 months at which point disease progression was noted. The subject was then treated with 11 cycles of Alimta" (Permetrexed) over 8 months, at which time treatment was halted due to the development of renal failure of undetermined etiology, Six months later, progressive disease was noted and the patient was again treated with Tarceva@ (Erlotinib) for 3 months. At that point, her CAT scan showed further progressive disease in the lungs with an increase in size of a right upper-lobe mass, pulmonary nodules consistent with metastases, and increasing intra-thoracic adenopathy. 100431 The subject was then enrolled in a trial using XilonixMN. MABpl (3.75 mg.kg) was infused intravenously every 21 days for 9 cycles, After treatment, stable disease was noted for approximately 30 weeks, and in the most recent restaging the right lung lesion appeared to be cavitating. [00441 Example 6 - Treatment of Non-small Cell Lung Cancer with an IL-la specific MAb (Xilonix"m). 100451 The subject was a 52 yr old female diagnosed with KRAS-positive non-small cell (adenocarcinoma) cancer of the lung on day 0. A PET/CT scan from day 14 revealed a 4 x 3,5 cm left upper lobe mass, with disease metastasis to the lungs, 13 hilar nodes, right inguinal nodes, right adrenal, 4th right rib, and sacroiliac joint. The subject began treatment with Carboplatin, Paclitaxel, and Bevacizumab a few weeks after the scan. The subject had a good initial response and completed five cycles before progressing at about 5 months from the first treatment. Over the next six months, the subject was treated with 3 cycles of Docetaxel and one cycle of Carboplatin plus Pemetrexed. Despite this therapy, she continued to progress. [00461 The subject subsequently began treatment with MABpL After only 4 days the subject began experiencing a worsening of her headaches. These were initially attributed to sinusitis, but MRI revealed brain metastases. The investigator believed that these were likely present prior to beginning therapy, however, the subject came off of study after only one dose of MABp I to receive gamma-knife radiotherapy, The subject was seen in follow up twenty days after the initial MABpI dose, and reported a subjective improvement in symptoms with a decrease in her chest pain. Because of this, the investigator checked a chest x-ray, which showed "an. obvious decrease in the size of her lung lesions" after only one dose, A waiver was issued, and the subject resumed therapy. Forty-six days after the initial MABpl dose, the subject underwent restaging, and had a 6% decrease in the sum total diameter of lesions as graded by RECI-ST criteria, Other Embodiments [0047] It is to be understood that while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims, Other aspects, advantages, and modifications are within the scope of the following claims. What is claimed is; 14

Claims (11)

1. A method for decreasing the size of a cancerous tumor in a human subject having a cancer that has progressed after treatment with chemotherapy, radiotherapy, or biologic agents, the method comprising the step of administering to the subject a pharmaceutical composition comprising a pharmaceutically acceptable carrier and an amount of an anti-IL-1a antibody effective to reduce the size of the tumor in the subject.

2. The method of claim 1, wherein the anti-IL-1a antibody is a monoclonal antibody.

3. The method of claim 2, wherein the monoclonal antibody is an IgG1.

4. The method of claim 2, wherein the monoclonal antibody comprises a complementarity determining region of MABpl.

5. The method of claim 2 or claim 3, wherein the monoclonal antibody is MABpl.

6. The method of any one of claims 1 to 5, wherein the cancer is a KRAS-mutated cancer.

7. The method of any one of claims 1 to 5, wherein the cancer is associated with EBV infection.

8. The method of any one of claims 1 to 5, wherein the cancer is nasopharyngeal cancer.

9. The method of any one of claims 1 to 5, wherein the cancer is lung cancer.

10. The method of any one of claims 1 to 5, wherein the cancer is colorectal cancer.

11. Use of an anti-IL-la antibody in the preparation of a medicament for treating a cancer that has progressed after treatment with chemotherapy, radiotherapy, or biologic agents.