AU2015200651B2 - Theobromine in combination with an expectorant or a mucolytic for use in therapy - Google Patents

Abstract

An agent comprises theobromine and an expectorant, or an agent comprises theobromine and a mucolytic. The agents of the invention may be used as a combined preparation for simultaneous, sequential or separate use in the therapy of cough.

Description

WO 2012/025761 PCT/GB2011/051610 THEOBROMINE IN COMBINATION WITH AN EXPECTORANT OR A MUCOLYTIC FOR USE IN THERAPY Field of the Invention This invention relates to a drug combination, its composition and its use in therapy. 5 Background of the Invention Cough is a protective reflex. Persistent cough can be distressing. Over the-counter remedies are available but their effectiveness is doubtful. W098/42322 discloses the use of theobromine for the treatment of cough, to be given orally. 10 Usmeni et al., FASEB J. express article 10.1096, discloses that theobromine inhibits sensory nerve action and cough. Data are provided, showing effects following oral dosing in citric-acid induced cough in the guinea pig, and in the capsaicin cough challenge in humans, and following bathing of isolated guinea pig vagus nerve preparations. 15 Expectorants and mucolytic agents dissolve thick mucus and relieve respiratory difficulties. Expectorants reduce the viscosity of bronchial secretions thus increasing mucus flow that can then be removed by coughing. Mucolytic agents also thin mucus, but do so by breaking down the chemical structure of mucus molecules. 20 Expectorants and mucolytics thin the mucus protective barrier, which as a result maximizes the penetration of pro-tussive agents (such as citric acid, dust, allergens etc) into the lung and enhances the pro-tussive effect. Summary of the Invention Given that a mucolytic or an expectorant is potentially pro-tussive, it was 25 surprising to find that a mucolytic/expectorant and theobromine had synergy. Based on what is known about expectorants/mucolytics, it was expected that an expectorant/mucolytic would decrease the efficacy of theobromine. The invention is therefore based at least in part on data showing synergistic anti-tussive effects for theobromine combined with guaifenesin in a 30 citric acid-induced cough model. When expectorants and/or mucolytics are combined with theobromine, the effects are surprisingly potent and greater than the sum of the individual drugs, revealing that the combination has a substantially improved effect. Consequently, a considerably reduced dose of WO 2012/025761 PCT/GB2011/051610 2 both drugs can be given for an equivalent effect for each individual drug, so reducing side-effects and drug burden. Therefore, the present invention is an agent comprising theobromine and an expectorant, or an agent comprising theobromine and a mucolytic. 5 Description of the Fiqures Figure 1 shows the total number of coughs in guinea-pigs during a 10 minute exposure and 5 minute recovery period to citric acid (1 M), following pre treatment with either vehicle (p.o.), theobromine (10 mg kg-1, i.p), guaifenesin (30 mg/kg, p.o.) or theobromine (10 mg/kg, p.o.) combined with guaifenesin (30 10 mg/kg. p.o.). Each column represents the mean and the vertical bars the s.e. mean. Changes in total cough number in the presence of theobromine, guaifenesin and theobromine in combination with guaifenesin were compared to vehicle dosed animals using ANOVA followed by Dunnetts test to compare the means. **P<0.01, *P<0.05. 15 Description of the Invention Any suitable form of theobromine can be chosen. These include salts, prodrugs and active metabolites. The additional agent (expectorant or mucolytic) may be used in an amount that is already known for its use, although combination according to this invention means that a reduced dose may be 20 effective. A combination according to the invention may be provided in a single formulation or in separate formulations, for combined, simultaneous or sequential administration. Expectorants suitable for use in the invention are ambroxol, ammonium bicarbonate, ammonium carbonate, bromhexine, calcium iodide, carbocysteine, 25 guaiacol, guaicacol benzoate, guaiacol carbonate, guaiacol phosphate, guaifenesin, guaithylline, hydriodic acid, iodinated glycerol, potassium guaiacolsulfonate, potassium iodide, sodium citrate, sodium iodide, storax terebene, terpin, trifolium, Althea root, Antimony pentasulfide, Creosote, lpecacuanha (Syrup of ipecac), levoverbenone, Senega and Tyloxapol. 30 Preferably, the expectorant is guaifenesin. Mucolytic agents suitable for use in the invention are acetylcysteine, bromhexine, carbocysteine, domiodol, erdostine, letostine, lysozyme, mecysteine hydrochloride, mesna, sobrerol, stepronin, tiopronin, tyloxapol, WO 2012/025761 PCT/GB2011/051610 3 ambroxol, ammonium chloride, Dornase alfa, Eprazinone, Erdosteine, Letosteine and Neltenexine. In a preferred embodiment, an agent comprises theobromine, an expectorant and a mucolytic. 5 In a preferred embodiment, an agent of the invention consists essentially of theobromine and an expectorant, or consists essentially of theobromine and a mucolytic, or consists essentially of theobromine, an expectorant and a mucolytic. In a preferred embodiment, an agent of the invention consists of 10 theobromine and an expectorant as active agents, or consists of theobromine and a mucolytic as active agents, or consists of theobromine, an expectorant and a mucolytic as active agents. Any suitable form of theobromine can be chosen. As theobromine is present in cocoa, the theobromine may be added in the form of cocoa or 15 chocolate (provided the amount of theobromine is in a high enough concentration to provide a therapeutic effect in the combination). If the theobromine is added in the form of cocoa and the medicament combination is in the form of a liquid, then there is the added benefit that the medicament is pleasant tasting. 20 The compounds of the invention are preferably as combinations to be administered orally, for example as tables, troches, lozenges, aqueous or orally suspensions, dispersible powders or granules. Preferred pharmaceutical compositions of the invention are tablets and capsules. Liquid dispersions for oral administration may be syrups, emulsions and suspensions. More 25 preferably, the pharmaceutical composition of the combination is a pressed tablet or capsule with conventional excipients, examples of which are given below. Compositions of the combination intended for oral use may be prepared according to any method known to the art for the manufacture of pharmaceutical 30 compositions, and such compositions may contain one or more agents selected from the group consisting of sweetening agents, flavouring agents, colouring agents and preserving agents in order to provide pharmaceutically elegant and palatable preparations. Tablets contain the combined active ingredients in admixture with non-toxic pharmaceutically acceptable excipients which are WO 2012/025761 PCT/GB2011/051610 4 suitable for the manufacture of tablets. These excipients may be, for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example corn starch or alginic acid; binding agents, for example starch gelatin, 5 acacia, microcrystalline cellulose or polyvinyl pyrrolidone; and lubricating agents, for example magnesium stearate, stearic acid or talc. The tablets may be uncoated or they may be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl 10 monostearate or glyceryl distearate may be employed. Aqueous suspensions contain the combined active materials in admixture with excipients suitable for the manufacture of aqueous suspensions. Such excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydroxypropylmethylcellulose, sodium alginate, polyvinyl 15 pyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents may be a naturally occurring phosphatide, for example lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long-chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products 20 of ethylene oxide with partial esters derived from fatty acids, for example polyoxyethylene sorbitan monooleate. The aqueous suspensions may also contain one or more preservatives, for example ethyl or n-propyl p hydroxybenzoate, one or more colouring agents, one or more flavouring agents, and one or more sweetening agents, such as sucrose or saccharin. 25 Oily suspensions may be formulated by suspending the active ingredient in a vegetable oil, for example arachis oil, olive oil, sesame oil or coconut oil, polyoxyethylene hydrogenated castor oil, fatty acids such as oleic acid, or in a mineral oil such as liquid paraffin or in other surfactants or detergents. The oily suspensions may contain a thickening agent, for example beeswax, hard 30 paraffin or cetyl alcohol. Sweetening agents, such as those set forth above, and flavouring agents may be added to provide a palatable oral preparation. These compositions may be preserved by the addition of an antioxidant such as ascorbic acid.

WO 2012/025761 PCT/GB2011/051610 5 Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the combined active ingredients in admixture with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable sweetening, flavouring and colouring agents may also 5 be present. The combined pharmaceutical compositions of the invention may also be in the form of oil-in-water emulsions. The oily phase may be a vegetable oil, for example olive oil or arachis oil, or a mineral oil, for example liquid paraffin, or mixtures of these. Suitable emulsifying agents may be naturally occurring gums, 10 for example gum acacia or gum tragacanth, naturally occurring phosphatides, for example soya bean, lecithin, and esters or partial esters derived from fatty acids and hexitol anhydrides, for example sorbitan monooleate and condensation products of the said partial esters with ethylene oxide, for example polyoxyethylene sorbitan monooleate. The emulsions may also contain 15 sweetening and flavouring agents. Syrups and elixirs may be formulated with sweetening agents, for example glycerol, propylene glycol, sorbitol or sucrose. Such formulations may also contain a demulcent, a preservative, flavouring and colouring agents. Suspensions and emulsions may contain a carrier, for example a natural 20 gum, agar, sodium alginate, pectin, methylcellulose, carboxymethylcellulose, or polyvinyl alcohol. In a preferred embodiment, theobromine in combination with an expectorant and/or a mucolytic is to be administered via the oral route. Combined compositions according to the invention may be produced using 25 conventional formulation techniques. In particular, spray-drying may be used to produce microparticles comprising the active agent dispersed or suspended within a material that provides the controlled release properties. The process of milling, for example jet milling, may also be used to formulate the therapeutic composition. The manufacture of fine particles by 30 milling can be achieved using conventional techniques. The term "milling" is used herein to refer to any mechanical process which applies sufficient force to the particles of active material to break or grind the particles down into fine particles. Various milling devices and conditions are suitable for use in the production of the compositions of the invention. The selection of appropriate WO 2012/025761 PCT/GB2011/051610 6 milling conditions, for example, intensity of milling and duration, to provide the required degree of force, will be within the ability of the skilled person. Ball milling is a preferred method. Alternatively, a high pressure homogeniser may be used, in which a fluid containing the particles is forced through a valve at high 5 pressure, producing conditions of high shear and turbulence. Shear forces on the particles, impacts between the particles and machine surfaces or other particles, and cavitation due to acceleration of the fluid, may all contribute to the fracture of the particles. Suitable homogenisers include the EmulsiFlex high pressure homogeniser, the Niro Soavi high pressure homogeniser and the 10 Microfluidics Microfluidiser. The milling process can be used to provide the microparticles with mass median aerodynamic diameters as specified above. If hygroscopic, the active agent may be milled with a hydrophobic material, as stated above. If it is required, the microparticles produced by the milling step can then 15 be formulated with an additional excipient. This may be achieved by a spray drying process, e.g. co-spray-drying. In this embodiment, the particles are suspended in a solvent and co-spray-dried with a solution or suspension of the additional excipient. Preferred additional excipients include polysaccharides. Additional pharmaceutically effective excipients may also be used. 20 Compositions of the combination intended for inhaled, topical, intranasal, sublingual, intravenous, rectal and vaginal use may be prepared according to any method known to the art for the manufacture of pharmaceutical compositions. Any suitable pharmaceutically effective drug which is used for the 25 treatment of a respiratory disease may also be co-administered with compositions of the invention. For example, 2 -agonists, e.g. salbutamol, salmeterol and formoterol, may be formulated for co-administration. Additional anti-muscarinic compounds may also be co-administered. For example, ipratropium (e.g. ipratropium bromide) or tiotropium may be administered. 30 Additional therapeutic agents, including steroids, may also be co administered. Examples of suitable steroids include beclomethasone, dipropionate and fluticasone. Other suitable therapeutic agents suitable for co administration include mucolytics, matrix metalloproteinase inhibitors, leukotrienes, antibiotics, anti-infective agents, antineoplastics, peptides, WO 2012/025761 PCT/GB2011/051610 7 antitussives, nicotine, PDE4 inhibitors, elastase inhibitors and sodium cromoglycate. Therapy according to the invention may be conducted in generally known manner, depending on various factors, such as the sex, age or condition of the 5 patient, and the existence or otherwise of one or more concomitant therapies. The patient population may be important, for example, in the treatment of patients with liver disease. The amounts of theobromine in the combination in one unit dose may be from 1 mg to 3000 mg. Preferably, the unit dose is from 50 mg to 1500 mg. 10 More preferably, the unit dose is from 400 mg to 1000 mg to be dosed one to four times daily. In a preferred embodiment, theobromine is to be dosed in an amount from 1-1000 mg/kg, preferably from 5-50 mg/kg. In a preferred embodiment, the expectorant and/or mucolytic is to be 15 dosed in an amount from 1-1000 mg/kg, preferably from 1-100 mg/kg. The following study illustrates the invention. Study Test Doses Group 1 - vehicle control 20 Group 2 - theobromine (10 mg/kg, p.o.) Group 3 - guaifenesin (30 mg/kg, p.o.) Group 4 - theobromine (10 mg/kg, p.o.) + guaifenesin (30 mg/kg, p.o.) Methods Animals 25 Male Dunkin Hartley guinea pigs (400-510 g, supplied by Harlan UK Ltd) were used throughout the study. Protocol Twenty four guinea pigs were randomly allocated to one of the three treatment groups (see Table 1) according to the blinding code. The blinding 30 code was not revealed to the investigator until coughs from all of the animals had been tallied. Guinea pigs were dosed via oral gavage (dose volume 2 mL/kg) with theobromine administered 0.5 hours prior to citric acid exposure. Guaifenesin and vehicle were administered 60 min prior to citric acid exposure.

WO 2012/025761 PCT/GB2011/051610 8 Individual guinea pigs were placed in an exposure chamber with an airflow of 2 L/min at t -10 min prior to citric acid exposure to allow acclimatisation. At t = 0 min, cough responses were induced by exposure to citric acid aerosol (1 M) generated by an ultrasonic nebuliser at a nebulisation 5 rate of 0.6 mL/min for 10 min. Coughs were counted throughout the 10 min citric acid exposure and for a further 5 min post exposure. Table 1: Treatment groups for study 376 (n=6 guinea pigs per group). 10 Vehicle Group Treatment group I Treatment group 2 Treatment group 3 theobromine guaifenesin (30 Theobromine (10 mg/kgp.o.), 0.5h mg/kg, p.o.), 1h (10 mg/kg, p.o.) + pretreatment pretreatment guaifenesin, (16 mg/kg, p.o.), 0.Sh and 1h pretreatment respectively Results The mean number of citric acid induced cough responses recorded in the vehicle treated guinea pigs was 30 ± 4 coughs. Pre-treatment with theobromine 15 (10 mg/kg, p.o.) caused a significant (P<0.05) reduction to the number of citric acid induced coughs (15 ± 3). In contrast though, guaifenesin (30 mg/kg, p.o.) had no significant effect on the total number of coughs (28 ± 3) evoked by citric acid exposure. In combination with guaifenesin (30 mg/kg, p.o.), the inhibitory effect of theobromine on the citric acid-induced tussive activity, showed a trend 20 to be potentiated in respect to total number of coughs observed (13 ± 3 c.f. 15 3). This synergy is shown in Figure 1.

Claims (20)

1. An agent consisting of theobromine and an expectorant, or theobromine and a mucolytic, or theobromine, an expectorant and a mucolytic as a combined preparation, wherein the agent is formulated for simultaneous administration of the theobromine with the expectorant; or wherein the agent is formulated for simultaneous administration of the theobromine with the mucolytic; or wherein the agent is formulated for simultaneous administration of the theobromine with the expectorant and the mucolytic.

2. An agent consisting of theobromine and an expectorant, or theobromine and a mucolytic, or theobromine, an expectorant and a mucolytic as a combined admixture preparation.

3. An agent consisting of theobromine and an expectorant, or theobromine and a mucolytic, or theobromine, an expectorant and a mucolytic as a combined preparation, wherein the agent is formulated for simultaneous administration of the theobromine with the expectorant, or wherein the agent is formulated for simultaneous or sequential administration of the theobromine with the mucolytic, or wherein the agent is formulated for simultaneous administration of the theobromine with the expectorant and the mucolytic, for therapy of cough.

4. An agent consisting of theobromine and an expectorant, or theobromine and a mucolytic, or theobromine, an expectorant and a mucolytic as a combined admixture preparation for therapy of cough.

5. An agent according to claim 1 or claim 3, consisting of theobromine, an expectorant and a mucolytic, wherein the agent is formulated for simultaneous administration of the theobromine with the expectorant and the mucolytic . 10

6. An agent according to claim 2 or claim 4, consisting of theobromine, an expectorant and a mucolytic as a combined admixture preparation.

7. The agent according to any one of claims 1-6, wherein the expectorant is ambroxol, ammonium bicarbonate, ammonium carbonate, bromhexine, calcium iodide, carbocysteine, guaiacol, guaiacol benzoate, guaiacol carbonate, guaiacol phosphate, guaifenesin, guaithylline, hydriodic acid, iodinated glycerol, potassium guaiacolsulfonate, potassium iodide, sodium citrate, sodium iodide, storax, terebene, terpin, trifolium, Althea root, antimony pentasulfide, creosote, ipecacuanha, levoverbenone, senega or tyloxapol.

8. The agent according to any one of claims 1-7, wherein the mucolytic is acetylcysteine, bromhexine, carbocysteine, domiodol, erdostine, letostine, lysozyme, mecysteine hydrochloride, mesna, sobrerol, stepronin, tiopronin, tyloxapol, ambroxol, ammonium chloride, dornase alfa, eprazinone, erdosteine, letosteine or neltenexine.

9. The agent according to any one of claims 1-8, wherein the theobromine is an amount of between 1 and 3000 mg.

10. The agent according to claim 9, wherein the theobromine is in an amount of between 50 and 1500 mg.

11. The agent according to claim 10, wherein the theobromine is in an amount of between 400 mg to 1000 mg.

12. The agent according to any one of claims 1-11 manufactured for oral administration.

13.The agent according to any one of claims 1-12 characterized by being prepared as a tablet, capsule, troche, lozenge, powder, granule, suspension, syrup or emulsion. 11

14. A pharmaceutical composition comprising an agent as defined in any one of claims 1 11 and one or more excipients.

15. The composition according to claim 14, further comprising one or more of sweetening agents, flavoring agents, coloring agents and/or preserving agents.

16.The composition according to claim 14 or claim 15, wherein the composition is manufactured for oral administration.

17.The composition according to any one of claims 14-16, wherein the composition is prepared as a tablet, capsule, troche, lozenge, powder, granule, suspension, syrup or emulsion.

18. Use of an agent as defined in any one of claims 1-13 or a pharmaceutical composition as defined in any one of claims 14-17 for the treatment of cough.

19. Use of an agent as defined in any one of claims 1-13 or a pharmaceutical composition as defined in any one of claims 14-17 for the manufacture of a medicament for treating cough.

20.A method for treating a cough, the method comprising the step of administering an agent as defined in any one of claims 1-13 or a pharmaceutical composition as defined in any one of claims 14-17.