AU2015100628A4 - Process for producing alcohol by fermentation of sugars - Google Patents

Abstract

The present invention relates to an improved process for producing alcohol by fermentation of sugars, 5 wherein the improvement to the process consists in the application of an electric field to the broth prepared before fermentation starts and during the entire fermentation process.

Description

WO 2014/015405 PCT/BR2013/000267 1 TITLE "PROCESS FOR PRODUCING ALCOHOL BY FERMENTATION OF SUGARS" 5 The present application is a divisional innovation application from Australian Patent Application number 2013296083, which claims priority from Brazilian basic application BR1120120185756, filed 26 July 2012, which 10 is hereby incorporated by reference in its entirety. Field of the invention This invention relates to a physical chemical process for improving the production of alcohol through the 15 fermentation of sugars which includes establishing polarisation in the fermentation broth by means of an electrical field. Background to the invention 20 Alcohols are a class of chemical compounds characterized in that they contain at least one hydroxyl group (OH) and they are extensively used in industry. The best known compound in this class is ethanol or ethyl alcohol. This can be found in 25 alcoholic drinks, cleaning products and pharmaceutical products and it is extensively used as a chemical solvent; it also has an application as a fuel for motor vehicles, which is currently its highest value and most intense use. 30 The process for producing ethanol is generally carried out on sugar cane, but it may also be carried out on a variety of grains and sources of sugar such as maize, cassava, other roots, sorghum, wheat, barley and molasses, syrup, cane bagasse, potatoes, whey, etc. 35 The manufacture of ethanol is basically divided into 4 stages: milling, liquefaction, fermentation and distillation. Milling comprises passage from the source of sugar by a processor. In this stage there is WO 2014/015405 PCT/BR2013/000267 2 obtained a broth which contains a high concentration of water and sugars. The fermentation part comprises the addition of some kind of yeast which causes the sugar present in the 5 solution to be converted into ethanol. It is the action of enzymes which carries out this work. After this process a fermented must is obtained, and this already contains part of its total volume converted into ethanol. 10 The must then continues on to the last stage, fractional distillation, and will give rise to a solution having the composition of ethanol and water. The stage most directly affecting the result of ethanol production, and therefore the most studied, is 15 fermentation, also known as alcoholic fermentation, which is the chemical process of converting sugars, mainly saccharose, glucose and fructose, into ethanol. Microbiological agents which are responsible for the conversion of sugars into ethanol take part in this 20 process. As it is a widely known process in the state of the art various documents describing processes for the purpose described above can be found. In general the processes concentrate on finding an ideal species or combination 25 of species for the production of ethanol, given that the process used involves a significant loss of the raw materials (sugars), thus reducing the efficiency of the process. Various documents dealing with different processes for 30 the production of ethanol can be found in the patent literature. American patent US 4451566 by Donald B. Spencer describes methods and equipment for the enzyme production of ethanol from fermentable sugars. A sequence of enzymes for catalysing conversion of the 35 sugars into ethanol is maintained in a variety of reaction zones. The fermentable sugar solution passes through these zones in sequence and alcohol is recovered in the last zone. Apart from providing a more WO 2014/015405 PCT/BR2013/000267 3 efficient reaction than the usual process, this document provides a solution which is onerous, complex and difficult to maintain. International patent application WO 2007/064545 by 5 Brian Burmaster describes a process for improving the yield of ethanol, reducing the fermentation time and reducing the formation of by-product by monitoring and controlling the oxidation-reduction potential of the fermenter. However this process requires very specific 10 monitoring and is difficult to maintain, causing the process to be expensive, even though it is more efficient. International patent application WO 2008/024331 by Vladimir Vlad describes a method for magnetic 15 fermentation which includes subjecting a biological material to a static magnetic field in order to affect fermentation of the biological material into a fermented product. The fermentation reaction can take place in alkaline or acid medium and the magnetic field 20 may be positive or negative. This document makes use of a static magnetic field to provide a more propitious environment for the cellular reproduction of microorganisms. Apart from increasing the number of microorganisms in the alcoholic fermentation and thus 25 increasing the reaction yield, this process requires constant monitoring and total control of the reaction, which renders the process expensive. Noting the limitations of the state of the art the inventor has developed a process for the production of 30 alcohol through the fermentation of sugars which brings benefits such as an increase in the efficiency of the process, through continuous electrical polarisation during the fermentation. 35 Summary of the invention Processes for the production of alcohol through the fermentation of sugars are already known to those skilled in the art. However all the processes used for WO 2014/015405 PCT/BR2013/000267 4 the production of alcohol through alcoholic fermentation either have substantial shortcomings in the yield from the fermentation reaction, giving rise to losses of raw material (sugars), or require 5 equipment or a process with a high cost of implementation or maintenance. Thus the object of this invention is to provide an improved low-cost physical chemical process for the production of alcohol through the fermentation of sugars which offers a high level of 10 utilization of the raw material (sugars) and their by products, consequently increasing the yield of the fermentation reaction. Fermentation processes are already well established, and involve a great cost of materials. Thus one object 15 of the invention is to provide a process which can be easily implemented in already installed facilities, providing better efficiency in the sugar fermentation process. 20 Detailed description of the invention This invention provides a physical chemical process for increasing and improving the yield of alcohol production through modifications and improvements in the stage of the fermentation of sugar-containing 25 solutions. This improvement comprises applying a direct current electrical field, causing dynamic polarisation of the sugars, together with electrolysis of the must, which in fact originates from the water present in the must which is almost wholly acidified. 30 The application of an electrical field to the processed broth (sugar-containing solution) has the result that polarisation is brought about in the molecules of the sugars present in the processed broth. The formation of these electrical polarisations increases the 35 selectivity of the sugar molecules, thus increasing the process yield. In order that this selectivity can take place with the desired efficiency the fermentation process must only WO 2014/015405 PCT/BR2013/000267 5 begin after the electrical field has been applied to the processed broth. Once all the molecules are electrically orientated the fermentation can begin. The electrodes described in this invention are devices 5 made of conducting metal. The process described in this invention comprises the following steps: 1. Preparing a container for the processed broth containing at least 2 electrodes; 10 2. Connecting the electrodes to a source of direct current; 3. Preparing the broth for fermentation; 4. Filling the container batchwise or continuously; 5. Connecting the electrodes to orientate the sugar 15 molecules; and 6. Starting the fermentation process. In one aspect of this invention the equipment includes means for controlling and maintaining fermentation parameters such as voltage and current. 20 A number of tests were performed to evaluate the efficacy applying the electrical field to the fermentation broth. One example of a process which is not intended to restrict the scope of protection of the invention follows below. 25 Example of a process for the production of ethanol through the fermentation of sugars. The process described below not only includes the application of an electrical field to the prepared 30 broth, but all the stages for the production of ethanol from sugar cane. We will consider a present-day case of a modern factory which has 12 (twelve) vats each of 300,000 liters, where this volume comprises cane broth, steeping water 35 plus the sum of chemical products, these products amounting on average to 6 x 10- 4 kg/l or 180 kg per vat of 300,000 liters.

WO 2014/015405 PCT/BR2013/000267 6 Through fermentation and distillation this factory produces 9 to 10% of ethanol from the 300,000 liters in each vat, that is 27,000 to 30,000 liters of ethanol per batch in each vat. 5 A direct current electrical field is introduced into each vat bringing about dynamic polarisation of the saccharose, together with electrolysis of the must, which in fact originates from water which is almost wholly acidified. Two types of gases are naturally 10 formed in this type of fermentation combined with electrolysis: - the fermentation gas, which is bubbles of carbon dioxide gas, - the electrolysis gas, where basically the gases in 15 acid medium are ionic in water with a small contribution from other gases relating to chemical elements present in the must in a very low concentration. This means that there will be an increase in the volume 20 of gases in the fermentation vats. Dynamic polarisation of the sugars plus the increase in volume of the gases and their reactivities will also give rise to a volume of ETHANOL and other compounds, in addition to those from simple alcoholic fermentation. 25 For the purposes of illustrating this invention the term TRS will be used to mean "total reducing sugars" . We will also use the term BRIX to mean the hydrometric scale for measuring the concentration of sugars in a particular solution at a particular temperature. These 30 terms are already well established in the state of the art and will be readily understood by a person skilled in the art. The pH in the vat at the start of fermentation is approximately 4.5. The BRIX for the must when 35 fermenting varies from 18 to 22 with an alcohol content of approximately 8%. When the fermentation starts the total reducing sugars, TRS, together with the BRIX are on a decreasing scale, their concentrations falling, WO 2014/015405 PCT/BR2013/000267 7 and the pH of the must which is becoming acidic decreases to 3.5 as the temperature rises. The temperature of the must should not rise, because this will cause the growth of microorganisms which will 5 consume some of the TRS still remaining in the process. Anodes and cathodes The anodes and cathodes are geometrically identical. They comprise coils with a sheet between the turns, 10 approximately equal to the diameter of the tubes, which should be of copper of 99.9% purity. These are made to the dimensions of the existing vats, which now already reach 10 (ten) meters in diameter and each have a capacity of 1000 m 3 of must. 15 The functions of the tubular-shaped ANODES and CATHODES comprise: - conducting the cathodic and anodic direct current; - bringing about cooling of the must through a flow of demineralized and deionised water in a closed circuit, 20 holding the temperature of the must below or equal to 35 0 C, using the water passing through the cathode and anode coils. The shape of the spirals depends on the shape of the vat, and they are present in pairs, and there must at 25 least be one pair. These spirals are supported on insulating material supports which do not allow electrical contact between them and the sides of the vats. The current circulating in these spirals may be switched in polarity in order to prevent the deposition 30 of anodic sludge on the anodes. The distance between the anode and the cathode will depend on the amperage and voltage, varying automatically in accordance with the changes in pH. As the pH of the must becomes acidic, an automatic 35 injection of a broth of lime or other alkalies is injected. Process gases WO 2014/015405 PCT/BR2013/000267 8 The fermentation gas is CO 2 (or carbon dioxide gas) which decreases with the fermentation process. When no more CO 2 forms it means that almost all the sugars have been converted and all the must has to proceed to 5 distillation. The reactive gases are ionic, capable of reacting in the formation of ethanol and other products. They take different forms and themselves maintain a pH around 4.5 and a temperature below 35 0 C, remaining approximately constant throughout all the 10 fermentation process even after CO 2 formation ceases with the natural end of fermentation. Thus part of the alcohol is also continuously drawn off in the form of vapor. These alcohol vapors should preferably be condensed and recovered without passing through 15 distillation of the final must. This will also allow capture of almost all the CO 2 , which is now discharged to the atmosphere. In this way the atmospheric emission of CO 2 , which is of the order of 1 cubic meter for each cubic meter of ethanol, will be substantially reduced. 20 Dynamic polarization of saccharose As the saccharose molecules are dynamically polarized they will always be orientated, aiding their conversion. 25 Automation of the process In automating the process pH must be controlled to the most appropriate level for each stage in the fermentation. This control will be implemented through 30 the pumps metering the lime broth or equivalent, because the trend during the course of the process is to further acidify the remaining fermenting must. Because of the change in pH during the fermentation process there will be changes in the density of the 35 electrical current, which will also have a system for measuring and controlling this, mainly in the batch process.

WO 2014/015405 PCT/BR2013/000267 9 Those skilled in the art will understand that the process described in this invention is not restricted to the specific features of the process of ethanol fermentation, and this process can be extended to each 5 and every fermentation process known in the state of the art, providing efficiency and speed and an increase in the production of alcohol.

Claims (5)

1. A process for the production of alcohol by the fermentation of sugars characterized in that it 5 comprises the application of an electrical field to the fermentation broth.

2. The process as claimed in claim 1, characterized in that said application of an electrical field is brought about through at least two electrodes. 10

3. The process as claimed in claim 2, characterized in that said electrodes comprise tubes with a geometry convenient for the circulation of water which also controls the process temperature of the fermenting must. 15

4. The process as claimed in claim 3, characterized in that said temperature lies within the range from 30 to 35 0 C.

5. The process as claimed in claim 1, characterized in that said pH varies from 6 to 4.5, in general of low 20 acidity.