AU2014367230A1 - Films of poly alpha-1,3-glucan esters and method for their preparation - Google Patents

Description

WO 2015/094402 PCT/US2014/044281 TITLE FILMS OF POLY ALPHA-1 3-GLUCAN ESTERS AND METHOD FOR THEIR PREPARATION THEREFROM This application is a Continuation-In-Part of United States Patent 5 Application 14/136168, filed December 20, 2013 and claims the benefit of U.S. Provisional Application Nos. 61/746,328; 61/746,335 and 61/746,338; each filed December 27, 2012, each now expired, all of which are incorporated herein by reference in their entirety. FIELD OF INVENTION 10 This invention is in the field of poly alpha-1,3-glucan derivatives. Specifically, this invention pertains to poly alpha-1,3-glucan esters, methods of their preparation and films made therefrom. BACKGROUND Driven by a desire to find new structural polysaccharides using enzymatic 15 syntheses or genetic engineering of microorganisms or plant hosts, researchers have discovered polysaccharides that are biodegradable, and that can be made economically from renewable resource-based feedstocks. One such polysaccharide is poly alpha-1,3-glucan, a glucan polymer characterized by having alpha-1,3-glycosidic linkages. This polymer has been isolated by 20 contacting an aqueous solution of sucrose with a glucosyltransferase enzyme isolated from Streptococcus salivarius (Simpson et al., Microbiology 141:1451 1460, 1995). Films prepared from poly alpha-1,3-glucan tolerate temperatures up to 150 0 C and provide an advantage over polymers obtained from beta-1,4 linked polysaccharides (Ogawa et al., Fiber Differentiation Methods 47:353-362, 25 1980). U.S. Patent 7,000,000 disclosed the preparation of a polysaccharide fiber comprising hexose units, wherein at least 50% of the hexose units within the polymer were linked via alpha-1,3-glycosidic linkages using an S. salivarius gtfJ enzyme. This enzyme utilizes sucrose as a substrate in a polymerization 30 reaction producing poly alpha-1,3-glucan and fructose as end-products (Simpson et al., 1995). The disclosed polymer formed a liquid crystalline solution when it 1 WO 2015/094402 PCT/US2014/044281 was dissolved above a critical concentration in a solvent or in a mixture comprising a solvent. From this solution, continuous, strong, cotton-like fibers, highly suitable for use in textiles, were spun and used. Information regarding preparation of various derivatives of poly alpha-1,3 5 glucan and their application is sparse. Yui et al. (/nt. J. Biol. Macromol. 14:87-96, 1992) disclose using poly alpha-1,3-glucan extracted from the fruiting body of the fungus, Laetiporus silphureus, to synthesize poly alpha-1,3-glucan triacetate. The structure of this polymer was analyzed by X-ray crystallography. 10 Ogawa et al. (Carb. Poly. 3:287-297, 1983) used three different samples of poly alpha-1,3-glucan to prepare poly alpha-1,3-glucan triacetate. One sample was isolated from a bacterial extracellular polysaccharide, and the other two samples were extracted from fruiting bodies of fungi. The structures of these polymers were analyzed by X-ray crystallography. 15 Development of new poly alpha-1,3-glucan ester derivatives and methods of preparing such derivatives is desirable given their potential utility in various applications, such as film production. SUMMARY OF INVENTION The present invention is directed toward a film comprising poly alpha-1,3 20 glucan ester having at least one of: (a) a tear resistance of at least about 0.1 gf/mil; or (b) a haze of less than about 20%. In another embodiment, the present invention is directed toward a method to prepare a poly alpha-1,3-glucan ester film comprising: (a) providing poly alpha 1,3-glucan ester; (b) contacting the poly alpha-1,3-glucan ester of (a) with a 25 solvent to make a solution of poly alpha-1,3-glucan ester; (c) applying the solution of poly alpha-1,3-glucan ester on a surface; and (d) allowing the solvent to evaporate to provide the poly alpha-1,3-glucan ester film. DETAILED DESCRIPTION OF INVENTION The disclosures of all patent and non-patent literature cited herein are 30 incorporated herein by reference in their entirety. 2 WO 2015/094402 PCT/US2014/044281 As used herein, the term "invention" or "disclosed invention" is not meant to be limiting, but applies generally to any of the inventions defined in the claims or described herein. These terms are used interchangeably herein. The terms "poly alpha-1,3-glucan", "alpha-1,3-glucan polymer" and 5 "glucan polymer" are used interchangeably herein. Poly alpha-1,3-glucan is a polymer comprising glucose monomeric units linked together by glycosidic linkages, wherein at least about 50% of the glycosidic linkages are alpha-1,3 glycosidic linkages. Poly alpha-1,3-glucan is a type of polysaccharide. The structure of poly alpha-1,3-glucan can be illustrated as follows: O~H C-1 H H H0 10 - . The poly alpha-1,3-glucan that can be used for preparing poly alpha-1,3 glucan ester compounds herein can be prepared using chemical methods. Alternatively, it can be prepared by extracting it from various organisms, such as fungi, that produce poly alpha-1,3-glucan. Alternatively still, poly alpha-1,3 15 glucan can be enzymatically produced from sucrose using one or more glucosyltransferase (gtf) enzymes (e.g., gtfJ), such as described in U.S. Patent No. 7,000,000, and U.S. Patent Appl. Publ. Nos. 2013/0244288 and 2013/0244287 (all of which are incorporated herein by reference), for example. The terms "glucosyltransferase enzyme", "gtf enzyme", "gtf enzyme 20 catalyst", "gtf', and "glucansucrase" are used interchangeably herein. The activity of a gtf enzyme herein catalyzes the reaction of sucrose substrate to make products poly alpha-1,3-glucan and fructose. Other products (byproducts) 3 WO 2015/094402 PCT/US2014/044281 of a gtf reaction can include glucose (where glucose is hydrolyzed from the glucosyl-gtf enzyme intermediate complex), various soluble oligosaccharides (DP2-DP7), and leucrose (where glucose of the glucosyl-gtf enzyme intermediate complex is linked to fructose). Leucrose is a disaccharide composed of glucose 5 and fructose linked by an alpha-1,5 linkage. Wild type forms of glucosyltransferase enzymes generally contain (in the N-terminal to C-terminal direction) a signal peptide, a variable domain, a catalytic domain, and a glucan binding domain. A gtf herein is classified under the glycoside hydrolase family 70 (GH70) according to the CAZy (Carbohydrate-Active EnZymes) database 10 (Cantarel et al., Nucleic Acids Res. 37:D233-238, 2009). The percentage of glycosidic linkages between the glucose monomer units of poly alpha-1,3-glucan used to prepare poly alpha-1,3-glucan ester compounds herein that are alpha-1,3 is at least about 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% (or any integer value between 50% 15 and 100%). In such embodiments, accordingly, poly alpha-1,3-glucan has less than about 50%, 40%, 30%, 20%, 10%, 5%, 4%, 3%, 2%, 1%, or 0% (or any integer value between 0% and 50%) of glycosidic linkages that are not alpha-1,3. Poly alpha-1,3-glucan used to produce poly alpha-1,3-glucan ester compounds herein is preferably linear/unbranched. In certain embodiments, poly 20 alpha-1,3-glucan has no branch points or less than about 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, or 1 % branch points as a percent of the glycosidic linkages in the polymer. Examples of branch points include alpha-1,6 branch points, such as those present in mutan polymer. The terms "glycosidic linkage" and "glycosidic bond" are used 25 interchangeably herein and refer to the type of covalent bond that joins a carbohydrate (sugar) molecule to another group such as another carbohydrate. The term "alpha-1,3-glycosidic linkage" as used herein refers to the type of covalent bond that joins alpha-D-glucose molecules to each other through carbons 1 and 3 on adjacent alpha-D-glucose rings. This linkage is illustrated in 30 the poly alpha-1,3-glucan structure provided above. Herein, "alpha-D-glucose" is referred to as "glucose". 4 WO 2015/094402 PCT/US2014/044281 The terms "poly alpha-1,3-glucan ester compound", "poly alpha-1,3-glucan ester", and "poly alpha-1,3-glucan ester derivative" are used interchangeably herein. A poly alpha-1,3-glucan ester compound herein can be represented by the structure: OR RO OR Regarding the formula of this structure, n can be at least 6, and each R can independently be a hydrogen atom (H) or an acyl group. A poly alpha-1,3-glucan ester compound herein has a degree of substitution of about 0.05 to about 3.0. Poly alpha-1,3-glucan ester compounds disclosed herein are synthetic, 10 man-made compounds. A poly alpha-1,3-glucan ester compound is termed an "ester" herein by virtue of comprising the substructure -CG-O-CO-C-, where "-CG-" represents carbon 2, 4, or 6 of a glucose monomeric unit of a poly alpha-1,3-glucan ester compound, and where "-CO-C-" is comprised in the acyl group. 15 An "acyl group" group herein can be an acetyl group (-CO-CH 3 ), propionyl group (-CO-CH 2

-CH

3 ), butyryl group (-CO-CH 2

-CH

2

-CH

3 ), pentanoyl group

(-CO-CH

2

-CH

2

-CH

2

-CH

3 ), hexanoyl group (-CO-CH 2

-CH

2

-CH

2

-CH

2

-CH

3 ), heptanoyl group (-CO-CH 2

-CH

2

-CH

2

-CH

2

-CH

2

-CH

3 ), or octanoyl group

(-CO-CH

2

-CH

2

-CH

2

-CH

2

-CH

2

-CH

2

-CH

3 ), for example. The carbonyl group (-CO-) 20 of the acyl group is ester-linked to carbon 2, 4, or 6 of a glucose monomeric unit of a poly alpha-1,3-glucan ester compound. Regarding nomenclature, a poly alpha-1,3-glucan ester compound can be referenced herein by referring to the organic acid(s) corresponding with the acyl group(s) in the compound. For example, an ester compound comprising acetyl 25 groups can be referred to as a poly alpha-1,3-glucan acetate, an ester compound 5 WO 2015/094402 PCT/US2014/044281 comprising propionyl groups can be referred to as a poly alpha-1,3-glucan propionate, and an ester compound comprising butyryl groups can be referred to as a poly alpha-1,3-glucan butyrate. However, this nomenclature is not meant to refer to the poly alpha-1,3-glucan ester compounds herein as acids per se. 5 "Poly alpha-1,3-glucan triacetate" herein refers to a poly alpha-1,3-glucan ester compound with a degree of substitution by acetyl groups of 2.75 or higher. The terms "poly alpha-1,3-glucan monoester" and "monoester" are used interchangeably herein. A poly alpha-1,3-glucan monoester contains only one type of acyl group. Examples of such monoesters are poly alpha-1,3-glucan 10 acetate (comprises acetyl groups) and poly alpha-1,3-glucan propionate (comprises propionyl groups). The terms "poly alpha-1,3-glucan mixed ester" and "mixed ester" are used interchangeably herein. A poly alpha-1,3-glucan mixed ester contains two or more types of an acyl group. Examples of such mixed esters are poly alpha-1,3 15 glucan acetate propionate (comprises acetyl and propionyl groups) and poly alpha-1,3-glucan acetate butyrate (comprises acetyl and butyryl groups). The terms "reaction", "reaction composition", and "esterification reaction" are used interchangeably herein and refer to a reaction comprising poly alpha 1,3-glucan, at least one acid catalyst, at least one acid anhydride and at least 20 one organic acid. The reaction is substantially anhydrous. A reaction is placed under suitable conditions (e.g., time, temperature) for esterification of one or more hydroxyl groups of the glucose units of poly alpha-1,3-glucan with an acyl group from at least the acid anhydride, thereby yielding a poly alpha-1,3-glucan ester compound. 25 The terms "substantially anhydrous" and "anhydrous" are used interchangeably herein. Substantially anhydrous conditions are conditions in which there is less than about 1.5 wt% or 2.0 wt% water. Such conditions may characterize a reaction or a reaction component, for example. Herein, a poly alpha-1,3-glucan that is "acid-exchanged" has been treated 30 with acid to remove water from the poly alpha-1,3-glucan. An acid-exchange process for producing acid-exchanged poly alpha-1,3-glucan can comprise one 6 WO 2015/094402 PCT/US2014/044281 or more treatments in which the glucan is placed in an acid (e.g., organic acid) and then removed from the acid. The term "acid catalyst" as used herein refers to any acid that accelerates progress of an esterification reaction. Examples of acid catalysts are inorganic 5 acids such as sulfuric acid (H 2

SO

4 ) and perchloric acid (HCIO 4 ). The term "acid anhydride" as used herein refers to an organic compound that has two acyl groups bound to the same oxygen atom. Typically, an acid anhydride herein has the formula (R-CO) 2 0, where R is a saturated linear carbon chain (up to seven carbon atoms). Examples of acid anhydrides are acetic 10 anhydride [(CH 3

-CO)

2 O], propionic anhydride [(CH 3

-CH

2

-CO)

2 O] and butyric anhydride [(CH 3

-CH

2

-CH

2

-CO)

2 O]. The terms "organic acid" and "carboxylic acid" are used interchangeably herein. An organic acid has the formula R-COOH, where R is an organic group and COOH is a carboxylic group. The R group herein is typically a saturated 15 linear carbon chain (up to seven carbon atoms). Examples of organic acids are acetic acid (CH 3 -COOH), propionic acid (CH 3

-CH

2 -COOH) and butyric acid

(CH

3

-CH

2

-CH

2 -COOH). The term "degree of substitution" (DoS) as used herein refers to the average number of hydroxyl groups substituted in each monomeric unit (glucose) 20 of a poly alpha-1,3-glucan ester compound. Since there are three hydroxyl groups in each monomeric unit in poly alpha-1,3-glucan, the DoS in a poly alpha 1,3-glucan ester compound herein can be no higher than 3. "Contacting" herein can be performed by any means known in the art, such as dissolving, mixing, shaking, or homogenization, for example. Where 25 three or more reaction components are contacted with each other, such contacting can be done all at once or in stages (e.g., two components mixed before mixing in a third component). The "molecular weight" of poly alpha-1,3-glucan and poly alpha-1,3-glucan ester compounds herein can be represented as number-average molecular 30 weight (Mn) or as weight-average molecular weight (Mw). Alternatively, molecular weight can be represented as Daltons, grams/mole, DPw (weight average 7 WO 2015/094402 PCT/US2014/044281 degree of polymerization), or DPn (number average degree of polymerization). Various means are known in the art for calculating these molecular weight measurements, such as high-pressure liquid chromatography (HPLC), size exclusion chromatography (SEC), or gel permeation chromatography (GPC). 5 The terms "percent by volume", "volume percent", "vol%" and "v/v %" are used interchangeably herein. The percent by volume of a solute in a solution can be determined using the formula: [(volume of solute)/(volume of solution)] x 100%. The terms "percent by weight", "weight percentage (wt%)" and "weight 10 weight percentage (% w/w)" are used interchangeably herein. Percent by weight refers to the percentage of a material on a mass basis as it is comprised in a composition, mixture or solution. The terms "increased", "enhanced" and "improved" are used interchangeably herein. These terms may refer to, for example, a quantity or 15 activity that is at least 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 125%, 150%, 175%, or 200% (or any integer between 1 % and 200%) more than the quantity or activity for which the increased quantity or activity is being compared. Embodiments of the disclosed invention concern a composition 20 comprising a poly alpha-1,3-glucan ester compound represented by the structure: OR RO OR n. Regarding the formula of this structure, n can be at least 6, and each R can independently be an H or an acyl group. Furthermore, the poly alpha-1,3-glucan 25 ester compound has a degree of substitution of about 0.05 to about 3.0. 8 WO 2015/094402 PCT/US2014/044281 Each R group in the formula of the poly alpha-1,3-glucan ester compound can independently be an H or an acyl group. An acyl group herein can be an acetyl group, propionyl group, butyryl group, pentanoyl group, hexanoyl group, heptanoyl group, or octanoyl group, for example. Thus, an acyl group can 5 comprise a chain of 2 to 8 carbons; this chain preferably has no branches. Poly alpha-1,3-glucan ester compounds in certain embodiments disclosed herein may contain one type of acyl group. For example, one or more R groups ester-linked to the glucose group in the above formula may be a propionyl group; the R groups in this particular example would thus independently be hydrogen 10 and propionyl groups. As another example, one or more R groups ester-linked to the glucose group in the above formula may be an acetyl group; the R groups in this particular example would thus independently be hydrogen and acetyl groups. Certain embodiments of poly alpha-1,3-glucan ester compounds herein do not have a DoS by acetyl groups of 2.75 or more. 15 Alternatively, poly alpha-1,3-glucan ester compounds disclosed herein can contain two or more different types of acyl groups. Examples of such compounds contain two different acyl groups, such as (i) acetyl and propionyl groups (poly alpha-1,3-glucan acetate propionate, where R groups are independently H, acetyl, or propionyl), or (ii) acetyl and butyryl groups (poly 20 alpha-1,3-glucan acetate butyrate, where R groups are independently H, acetyl, or butyryl). The poly alpha-1,3-glucan ester compound has a degree of substitution (DoS) of about 0.05 to about 3.0. Alternatively, the DoS of a poly alpha-1,3 glucan ester compound disclosed herein can be about 0.2 to about 2.0. 25 Alternatively still, the DoS can be at least about 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, or 3.0. It would be understood by those skilled in the art that since a poly alpha-1,3-glucan ester compound disclosed herein has a degree of substitution between about 0.05 to about 3.0, the R groups of the compound 30 cannot only be hydrogen. 9 WO 2015/094402 PCT/US2014/044281 The wt% of one or more acyl groups in a poly alpha-1,3-glucan ester compound herein can be referred to instead of referencing a DoS value. For example, the wt% of an acyl group in a poly alpha-1,3-glucan ester compound can be at least about 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 5 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%. The percentage of glycosidic linkages between the glucose monomer 10 units of the poly alpha-1,3-glucan ester compound that are alpha-1,3 is at least about 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% (or any integer between 50% and 100%). In such embodiments, accordingly, the compound has less than about 50%, 40%, 30%, 20%, 10%, 5%, 4%, 3%, 2%, 1 %, or 0% (or any integer value between 0% and 50%) of glycosidic linkages that 15 are not alpha-1,3. The backbone of a poly alpha-1,3-glucan ester compound disclosed herein is preferably linear/unbranched. In certain embodiments, the compound has no branch points or less than about 10%, 9%, 8%, 7%, 6%, 5%, 4%, 3%, 2%, or 1 % branch points as a percent of the glycosidic linkages in the polymer. 20 Examples of branch points include alpha-1,6 branch points. The formula of a poly alpha-1,3-glucan ester compound in certain embodiments can have an n value of at least 6. Alternatively, n can have a value of at least 10, 50,100, 200, 300, 400, 500, 600, 700, 800, 900,1000,1100, 1200,1300,1400,1500,1600,1700,1800,1900,2000,2100,2200,2300,2400, 25 2500,2600,2700,2800,2900,3000,3100,3200,3300,3400,3500,3600,3700, 3800, 3900, or 4000 (or any integer between 10 and 4000). The molecular weight of a poly alpha-1,3-glucan ester compound disclosed herein can be measured as number-average molecular weight (Mo) or as weight-average molecular weight (Mw). Alternatively, molecular weight can be 30 measured in Daltons or grams/mole. It may also be useful to refer to the DPw (weight average degree of polymerization) or DPo (number average degree of 10 WO 2015/094402 PCT/US2014/044281 polymerization) of the poly alpha-1,3-glucan polymer component of the compound. The Mo or M, of poly alpha-1,3-glucan ester compounds disclosed herein may be at least about 1000. Alternatively, the Mo or M, can be at least about 5 1000 to about 600000. Alternatively still, the Mn or M, can be at least about 10000,25000,50000,75000,100000,125000,150000,175000,200000, 225000, 250000, 275000, or 300000 (or any integer between 10000 and 300000), for example. A poly alpha-1,3-glucan ester in certain embodiments can have a DoS by 10 acetyl groups up to about 2.00, 2.05, 2.10, 2.15, 2.20, 2.25, 2.30, 2.35, 2.40, 2.45, 2.50, 2.55, 2.60, 2.65, 2.70, 2.75, 2.80, 2.85, 2.90, 2.95, or 3.00. Thus, for example, the DoS by acetyl groups can be up to about 2.00-2.40, 2.00-2.50, or 2.00-2.65. As other examples, the DoS by acetyl groups can be about 0.05 to about 2.60, about 0.05 to about 2.70, about 1.2 to about 2.60, or about 1.2 to 15 about 2.70. Such poly alpha-1,3-glucan esters can be a monoester or a mixed ester. A poly alpha-1,3-glucan ester in certain embodiments can have a wt% of propionyl groups up to about 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 20 53%, 54%, or 55%. Such poly alpha-1,3-glucan esters can be a monoester or a mixed ester. Regarding mixed esters, poly alpha-1,3-glucan acetate propionate can have a wt% of acetyl groups up to about 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, or 10%, and a wt% of propionyl groups as per any of the propionyl wt%'s listed above, for example. 25 A poly alpha-1,3-glucan ester in certain embodiments can have a wt% of butyryl groups up to about 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 30 59%, or 60%. A poly alpha-1,3-glucan ester in other embodiments can have a DoS by butyryl groups up to about 0.80, 0.85, 0.90, 0.95, 1.00, 1.05, 1.10, 1.15, 11 WO 2015/094402 PCT/US2014/044281 or 1.20. Such poly alpha-1,3-glucan esters can be a monoester or a mixed ester. Regarding mixed esters, poly alpha-1,3-glucan acetate butyrate can have a wt% of acetyl groups up to about 0.1%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 5 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, or 36%, and a wt% of butyryl groups as per any of the butyryl wt%'s listed above, for example. The disclosed invention also concerns a method for producing a poly alpha-1,3-glucan ester compound. This method comprises: contacting poly alpha-1,3-glucan in a reaction that is substantially anhydrous with at least one 10 acid catalyst, at least one acid anhydride, and at least one organic acid, wherein an acyl group derived from the acid anhydride is esterified to the poly alpha-1,3 glucan thereby producing a poly alpha-1,3-glucan ester compound represented by the structure: OR RO OR 15 wherein (i) n is at least 6, (ii) each R is independently an H or the acyl group, and (iii) the compound has a degree of substitution of about 0.05 to about 3.0. A poly alpha-1,3-glucan ester produced by this method can optionally be 20 isolated. A poly alpha-1,3-glucan is contacted with at least one acid catalyst, at least one acid anhydride, and at least one organic acid in a reaction that is substantially anhydrous. A substantially anhydrous reaction herein contains no water or less than about 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 25 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, or 2.0 wt% water. Substantially anhydrous 12 WO 2015/094402 PCT/US2014/044281 conditions can be obtained by using reaction components that are substantially anhydrous. Reaction components that are not substantially anhydrous may be used for preparing a reaction, but only in amounts such that the final reaction preparation is substantially anhydrous. 5 Enzymatically produced preparations of poly alpha-1,3-glucan that can be used in the disclosed esterification reaction typically contain water. This poly alpha-1,3-glucan can be acid-exchanged to remove water thereby rendering the glucan to be substantially anhydrous. In certain embodiments, poly alpha-1,3 glucan can be acid-exchanged with an organic acid (e.g., acetic, propionic, or 10 butyric acid) before contacting step (a) to remove water from the poly alpha-1,3 glucan. An acid-exchange process herein can comprise boiling poly alpha-1,3 glucan in water, removing most of the water by any physical means (e.g., filtration, decantation, and/or drying), placing the glucan in an organic acid, and then removing the organic acid by filtration and/or decantation. Treatment with 15 an organic acid can comprise stirring the glucan in the acid, and can be performed one, two, or more times. The amount of organic acid used in each treatment can be at least about 2 to 20 times, or 2 to 10 times, the amount of poly alpha-1,3-glucan being treated, for example. Poly alpha-1,3-glucan is contacted with at least one acid catalyst in the 20 disclosed reaction. An acid catalyst can be an inorganic acid in certain embodiments. Examples of an inorganic acid catalyst that can be included in a reaction herein are sulfuric acid and perchloric acid. Other examples of inorganic acid catalysts include hydrochloric, phosphoric, nitric, boric, hydrofluoric, hydrobromic, sulfonic, any mineral acid, and any combination thereof. An acid 25 catalyst herein can typically be obtained commercially in a concentrated (e.g., >95%, 96%, 97%, 98%, or 99% pure) and/or substantially anhydrous form. For example, sulfuric acid for use in a reaction herein can be at least about 95-98% pure. Alternatively, an acid catalyst can be provided in solution with an organic acid such as acetic acid. An example of such a solution is perchloric acid (0.1 N) 30 in acetic acid. The amount of acid catalyst in a reaction can be at least about 13 WO 2015/094402 PCT/US2014/044281 0.005, 0.0075, 0.01, 0.025, 0.05, 0.075, 0.1, 0.25, 0.5, 0.75, 1.0, 1.25, 1.5, 1.75, or 2.0 wt%, for example. Poly alpha-1,3-glucan is contacted with at least one acid anhydride in the disclosed reaction. Examples of an acid anhydride that can be included in a 5 reaction herein include acetic anhydride, propionic anhydride, butyric anhydride, pentanoic anhydride, hexanoic anhydride, heptanoic anhydride, octanoic anhydride and phthalic anhydride. Any combination of these can be used in a reaction herein (e.g., acetic and propionic anhydrides, acetic and butyric anhydrides, propionic and butyric anhydrides). An acid anhydride herein can 10 typically be obtained commercially in a concentrated (e.g., >95%, 96%, 97%, 98%, or 99% pure) and/or substantially anhydrous form. For example, acetic anhydride, propionic anhydride and/or butyric anhydride for use in a reaction herein can be at least about 97%, 98%, or 99% pure. The amount of acid anhydride in a reaction can be at least about 10, 15, 20, 25, 30, 35, 40, 45, 50, 15 55, 60, 65, or 70 wt% (or any integer value between 10 and 70 wt%), for example. In certain embodiments, the amount of acetic anhydride in a reaction can be at least about 20-45 wt%. The amount of propionic or butyric anhydride in other embodiments can be at least about 40-50 wt%. Poly alpha-1,3-glucan is contacted with at least one organic acid in the 20 disclosed reaction. Examples of an organic acid that can be included in a reaction herein include acetic acid, propionic acid, butyric acid, pentanoic acid, hexanoic acid, heptanoic acid, octanoic acid and phthalic acid. The amount of organic acid in a reaction can be at least about 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, or 80 wt% (or any integer value between 5 and 80 wt%), 25 for example. Typically, one or more acid anhydrides used in a reaction herein are selected based on the type of esterification desired. As examples, if esterification of poly alpha-1,3-glucan with acetyl groups, propionyl groups and/or butyryl groups is desired, then acetic anhydride, propionic anhydride and/or 30 butyric anhydride, respectively, is/are included in the reaction accordingly. The selected acid anhydride(s) is the main source of acyl groups in the disclosed 14 WO 2015/094402 PCT/US2014/044281 esterification process. That being said, acyl groups for esterification can also be derived from one or more organic acids included in the reaction. For example, an acetyl group, propionyl group, butyryl group, pentanoyl group, hexanoyl group, heptanoyl group, and octanoyl group can be derived from, respectively, 5 acetic acid, propionic acid, butyric acid, pentanoic acid, hexanoic acid, heptanoic acid, and octanoic acid. Reactions containing a particular acid anhydride typically also contain the organic acid corresponding to the acid anhydride. In certain embodiments of the disclosed reaction, the acid anhydride is one or more of acetic anhydride, propionic anhydride, or butyric anhydride; and 10 the organic acid is one or more of acetic acid, propionic acid, or butyric acid. Combinations of (i) acetic anhydride and acetic acid can be used to prepare poly alpha-1,3-glucan acetate; (ii) propionic anhydride and propionic acid can be used to prepare poly alpha-1,3-glucan propionate; (iii) butyric anhydride and butyric acid can be used to prepare poly alpha-1,3-glucan butyrate; (iv) propionic 15 anhydride, propionic acid, acetic anhydride and optionally acetic acid can be used to prepare poly alpha-1,3-glucan acetate propionate; (v) propionic anhydride, propionic acid and acetic acid can be used to prepare poly alpha-1,3 glucan acetate propionate; (vi) butyric anhydride, butyric acid, acetic anhydride and optionally acetic acid can be used to prepare poly alpha-1,3-glucan acetate 20 butyrate; and (vii) butyric anhydride, butyric acid and acetic acid can be used to prepare poly alpha-1,3-glucan acetate butyrate, for example. In reactions containing acetic acid along with propionic acid or butyric acid, the amount of acetic acid can be about 5-10, 5-20, or 5-30 wt%, for example. Reactions for producing mixed esters (e.g., poly alpha-1,3-glucan acetate 25 propionate, poly alpha-1,3-glucan acetate butyrate) typically contain more of an acid anhydride having an acyl group for which a higher DoS is desired, and less of an acid anhydride and/or corresponding organic acid for which a lower DoS is desired. For example, to produce a poly alpha-1,3-glucan acetate propionate with a higher DoS of propionyl groups compared to acetyl groups, more propionic 30 anhydride is included in a reaction compared to the amount of acetic anhydride and/or acetic acid. DoS in mixed esters may also be modulated by the order in 15 WO 2015/094402 PCT/US2014/044281 which acid anhydrides are added to a reaction already containing an acid catalyst. For example, one may expect a higher DoS with propionyl groups if propionic anhydride is added before acetic anhydride (to a preparation already containing acid catalyst) when preparing a reaction to produce poly alpha-1,3 5 glucan acetate propionate. An acid anhydride selected for a reaction herein can correspond with the organic acid used to prepare acid-exchanged poly alpha-1,3-glucan. For example, if a reaction will include propionic anhydride, then an acid exchange process can be performed with propionic acid. Alternatively, an acid anhydride 10 selected for a reaction herein can differ from the organic acid used to prepare acid-exchanged poly alpha-1,3-glucan. For example, if a reaction will include propionic anhydride, then an acid exchange process can be performed with acetic acid. A reaction herein can comprise components in addition to poly alpha-1,3 15 glucan, acid catalyst, acid anhydride, and organic acid. For example, one or more organic solvents can be included in a reaction, such as methylene chloride. An organic solvent such a methylene chloride can be included at about 30-40 wt% in a reaction (e.g., producing glucan triacetate), for example. The components of a reaction herein can be added together in any order. 20 For example, poly alpha-1,3-glucan, acid catalyst and organic acid can first be mixed together, afterwhich acid anhydride can be added to the mixture. As another example, acid anhydride and organic acid can first be mixed together, afterwhich poly alpha-1,3-glucan and acid catalyst can be added to the mixture. As yet another example, acid catalyst and organic acid can first be mixed 25 together, afterwhich poly alpha-1,3-glucan and acid anhydride can be added to the mixture. In certain embodiments, poly alpha-1,3-glucan and another component (e.g., acid catalyst or acid anhydride) are added in sequential order to a mixture containing the other reaction components. Cooling can be applied during various stages of preparing a reaction 30 herein. The terms "cool" and "chill" are used interchangeably herein and refer to decreasing the temperature of a reaction or mixture to a lower temperature. 16 WO 2015/094402 PCT/US2014/044281 Cooling can be performed by any means known in the art, such as with an ice bath or industrial cooling system. Step (a) of preparing a reaction can comprise cooling the reaction after its preparation (i.e., containing all of poly alpha-1,3 glucan, acid catalyst, acid anhydride and organic acid), such as to about 10, 11, 5 12, 13, 14, 15, 16, 17, 18, 19, or 20 0C, or about 12-18 C. Alternatively, step (a) can comprise cooling (e.g., to any of the preceding temperatures) a mixture containing poly alpha-1,3-glucan, acid catalyst and organic acid, and then adding acid anhydride to the cooled mixture. Alternatively still, step (a) can comprise cooling (e.g., to any of the preceding temperatures) a mixture containing acid 10 anhydride and organic acid, and then adding poly alpha-1,3-glucan and acid catalyst to the cooled mixture. Alternatively still, step (a) can comprise cooling (e.g., to any of the preceding temperatures) a mixture containing acid catalyst and organic acid, and then adding poly alpha-1,3-glucan and acid anhydride to the cooled mixture. A reaction can optionally be held at any of the preceding 15 cooler temperature points for about 1-10 minutes after its initial preparation. A reaction can then be (i) placed under ambient temperature conditions without direct application of heat, and/or (ii) directly heated using any means known in the art (e.g., water bath, industrial or electric heater). Ambient temperature conditions can be held for up to about 30, 60, 120, 240, 360, or 480 20 minutes (or any integer value between 30 and 480 minutes), for example. Alternatively, ambient temperature conditions can be held for up to about 24, 48, or 72 hours. The term "ambient temperature" as used herein refers to a temperature between about 15-30 0C or 20-25 0C (or any integer between 15 and 30 C). Reaction heating can be up to about 30, 35, 40, 45, 50, 55, 60, 65, 70, 25 75, or 80 0C (or any integer value between 30 and 80 C), about 30-60 C, or about 30-50 C, for example. Such heating can be done is stages, if desired. For example, a reaction can first be heated to about 35 C, and then heated to about 39-50 C. A maximum reaction temperature (e.g., about 36-43 0C) may be applied to avoid excess degradation of poly alpha-1,3-glucan ester molecular 30 weight in certain embodiments, such as when producing poly alpha-1,3-glucan propionate, poly alpha-1,3-glucan acetate propionate, or poly alpha-1,3-glucan 17 WO 2015/094402 PCT/US2014/044281 acetate butyrate. The temperature after heating to any of the preceding temperatures can be maintained for about 20-30, 20-40, 20-60 minutes, or up to about 40, 60, 80, 100, 120, or 140 minutes, for example. When heating is done in stages, the first temperature point(s) can be held for about 20-40 minutes, for 5 example. In embodiments in which a reaction is placed under ambient temperature conditions without direct application of heat, the reaction can subsequently be heated, if desired, to any of the preceding temperatures and time periods. A reaction typically does not contain any solid material, but may be viscous, after any of the above temperature treatments (ambient temperature 10 and/or heating). A reaction can optionally be cooled after any of the above temperature treatments (ambient temperature and/or heating). For example, a reaction can be cooled to about 18, 19, 20, 21, 22, 23, 24, or 25 0C, about 20-30 C, or about 20-40 OC. A reaction that was heated to 60-80 0C can typically be cooled to 15 about 35-45 OC. The temperature of a reaction upon cooling can be held for about 5-10 minutes, for example. Optionally, a reaction herein can be maintained under an inert gas (e.g., nitrogen). As used herein, the term "inert gas" refers to a gas which does not undergo chemical reactions under a set of given conditions, such as those 20 disclosed for preparing a reaction herein. A reaction can optionally be quenched after any of the above temperature treatments (ambient temperature and/or heating) and cooling treatments. Quenching of a reaction can be accomplished by adding acid, base, or certain salts to the reaction. Various acids, bases and salts useful for quenching a 25 reaction include, but are not limited to, acetic acid (e.g., -50-70 wt%), any other mineral or organic acid (e.g., -50-70 wt%), magnesium acetate (e.g., -20-25 wt%), sodium hydroxide, potassium hydroxide, calcium hydroxide, sodium bicarbonate, sodium carbonate and combinations thereof. In certain embodiments of producing poly alpha-1,3-glucan acetate, a reaction is quenched 30 with acetic acid (e.g., -50 or 70 wt%) or magnesium acetate (e.g., -20-25 wt%). 18 WO 2015/094402 PCT/US2014/044281 A quenched reaction can optionally be heated to about 40 0C to 150 0C for up to 48 hours. For example, a quenched reaction can be heated to about 100 0C for up to about 20-40 minutes (e.g., 25-30 minutes), such as in a process for producing poly alpha-1,3-glucan acetate. Optionally, water may be added to a 5 reaction (quenched or not quenched), which is then heated to about 40 0C to 150 0C (e.g., -100 0C) for up to about 20-40 minutes (e.g., 25-30 minutes) to reduce DoS of acyl groups by hydrolysis. Such a heating/water-treatment step may be useful for reducing DoS in a process for producing poly alpha-1,3-glucan acetate. A poly alpha-1,3-glucan ester compound produced by a reaction herein 10 can be precipitated using an agent that is a non-solvent for the poly alpha-1,3 glucan ester compound. For example, deionized water and/or methanol can be added to a reaction solution in an amount sufficient to precipitate a poly alpha 1,3-glucan ester compound. Precipitation herein can further comprise mixing the reaction solution and non-solvent by any means known in the art, such as with an 15 air-powered blender. Precipitated poly alpha-1,3-glucan ester compound can optionally be neutralized by washing it with water two or more times, followed by a wash in a bicarbonate (e.g., sodium bicarbonate) solution (e.g., -5 wt%). The ester compound can then be washed one, two or more times with water until neutral 20 pH is achieved. Alternatively, precipitated poly alpha-1,3-glucan ester compound can be washed with water and base (e.g., diluted alkaline hydroxide such as sodium hydroxide, calcium hydroxide, or potassium hydroxide) to achieve a neutral pH, optionally followed by washing with water. The term "neutral pH" as used herein refers to a pH that is neither substantially acidic or basic (e.g., a pH 25 of about 6-8, or about 6.0, 6.2, 6.4, 6.6, 6.8, 7.0, 7.2, 7.4, 7.6, 7.8, or 8.0). A poly alpha-1,3-glucan ester produced in the disclosed reaction can be isolated. The above precipitation process can be a step in an isolation process. Isolation can be performed with precipitated product before or after neutralization and/or washing steps using a funnel, centrifuge, press filter, or any other method 30 or equipment known in the art that allows removal of liquids from solids. An isolated poly alpha-1,3-glucan ester product can be dried using any method 19 WO 2015/094402 PCT/US2014/044281 known in the art, such as vacuum drying, air drying (e.g., -16-35 0C), or freeze drying. Any of the above esterification reactions can be repeated using a poly alpha-1,3-glucan ester product as the starting material for further modification. 5 This approach may be suitable for increasing the DoS of an acyl group, and/or adding one or more different acyl groups to the ester product. The structure, molecular weight and DoS of a poly alpha-1,3-glucan ester product can be confirmed using various physiochemical analyses known in the art such as NMR spectroscopy and size exclusion chromatography (SEC). 10 The percentage of glycosidic linkages between the glucose monomer units of poly alpha-1,3-glucan used to prepare poly alpha-1,3-glucan ester compounds herein that are alpha-1,3 is at least about 50%, 60%, 70%, 80%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% (or any integer value between 50% and 100%). In such embodiments, accordingly, poly alpha-1,3-glucan has less 15 than about 50%, 40%, 30%, 20%, 10%, 5%, 4%, 3%, 2%, 1%, or 0% (or any integer value between 0% and 50%) of glycosidic linkages that are not alpha-1,3. Poly alpha-1,3-glucan used to prepare poly alpha-1,3-glucan ester compounds herein is preferably linear/unbranched. In certain embodiments, poly alpha-1,3-glucan has no branch points or less than about 10%, 9%, 8%, 7%, 6%, 20 5%, 4%, 3%, 2%, or 1 % branch points as a percent of the glycosidic linkages in the polymer. Examples of branch points include alpha-1,6 branch points. The Mn or M, of poly alpha-1,3-glucan used to prepare poly alpha-1,3 glucan ester compounds herein may be at least about 500 to about 300000. Alternatively still, Mo or M, can be at least about 10000, 25000, 50000, 75000, 25 100000,125000,150000,175000,200000,225000,250000,275000,or300000 (or any integer between 10000 and 300000), for example. A process is disclosed herein for producing poly alpha-1,3-glucan acetate with a DoS of 0.05 to 2.70 using poly alpha-1,3-glucan triacetate. The triacetate used in this process can be produced according to any of the above processes, 30 for example. This process comprises: contacting poly alpha-1,3-glucan triacetate with acetic acid and water to form a preparation, and applying steam 20 WO 2015/094402 PCT/US2014/044281 pressure of about 3-10 kg/cm 2 to the preparation to raise its temperature up to about 260 OC. This process results in a poly alpha-1,3-glucan acetate having a DoS of 0.05 to 2.70. Such reduction in DoS results from hydrolysis of a portion of the acetyl groups of the poly alpha-1,3-glucan triacetate. A poly alpha-1,3 5 glucan acetate produced by this method can optionally be isolated. Poly alpha-1,3-glucan triacetate can optionally be washed and/or have a neutral pH prior to use in this process. Poly alpha-1,3-glucan triacetate can be contacted with acetic acid and water by first dissolving the glucan triacetate in acetic acid, and then adding water to this solution. In certain embodiments, the 10 amount of acetic acid in the preparation can be about 75, 80, 85, or 90 wt%, and the amount of water in the preparation can be up to about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 wt%. A preparation containing poly alpha-1,3-glucan triacetate, acetic acid and water is then subjected to steam pressure of about 3-10 kg/cm 2 to raise its 15 temperature up to about 260 OC. This step can be optionally be carried out in a pressure vessel such as a Parr reactor, autoclave, or any other pressure vessel known in the art. A steam pressure of about 4, 5, or 6 kg/cm 2 , for example, can be used to raise the temperature of the preparation to about 140-160 C (e.g., 150 0C). This elevated temperature can be held for about 30, 40, 50, 60, or 70 20 minutes, afterwhich the applied pressure can be increased further to about 7, 8, or 9 kg/cm 2 . After reaching this elevated pressure, the temperature can be cooled to ambient temperature. Poly alpha-1,3-glucan acetate having a DoS of 0.05 to 2.70 can be isolated from the pressure- / heat-treated preparation using any of the 25 precipitation, washing and isolation steps disclosed above. Poly alpha-1,3-glucan esters formed using various methods described above can be used to prepare various types of films. The poly alpha-1,3-glucan esters prepared according to the disclosed methods can be dissolved in one or more solvents to provide a solution of poly alpha-1,3-glucan ester. As used 30 herein, the term "solution of poly alpha-1,3-glucan ester" refers to poly alpha-1,3 glucan ester dissolved in one or more solvents. The solvents useful for this 21 WO 2015/094402 PCT/US2014/044281 purpose include, but are not limited to, methylene chloride (dichloromethane), methanol, chloroform, tetrachloroethane, formic acid, acetic acid, nitrobenzene, bromoform, pyridine, dioxane, ethanol, acetone, alcohols, aromatic compounds such as monochlorobenzene, benzene and toluene, esters such as ethyl acetate 5 and propyl acetate, ethers such as tetrahydrofuran, methyl cellosolve and ethylene glycol monomethyl ether or combinations thereof. In an embodiment poly alpha-1,3-glucan acetate is dissolved in acetone to prepare a solution of poly alpha-1,3-glucan acetate. This solution can then be applied to a surface and the solvent is allowed to evaporate to form a film of desired thickness. The 10 surfaces suitable for this application can be, but are not limited to, glass, Teflon@, plastic, or various types of substrates. Methods to make films from the above mentioned solution, which are well known in the art, include but not limited to solution casting, spin coating, thermal and regular spraying. In an embodiment, the solution of poly alpha-1,3-glucan ester is cast on a glass plate. 15 The tear resistance, tensile strength and temperature stability of the poly alpha-1,3-glucan ester films can be determined by methods well known in the art. As used herein, the term "tear resistance" is defined as a measure of how well a film can withstand the effects of tearing. The term "tensile strength", as used herein, refers to the maximum tension a material can withstand without tearing. 20 The suitable tear resistance for a poly alpha-1,3-glucan ester film disclosed herein can be at least 0.1 gf/mil. The tensile strength of the film suitable for the disclosed invention can be at least 4 kgf/mm 2 . In an embodiment, the tear resistance of the poly alpha-1,3-glucan diacetate film is 2-2.4 gf/mil and the tensile strength is 3.97-4.8 kgf/mm 2 . In another embodiment, the tear resistance 25 is 1.4-3.1 gf/mil and the tensile strengthis 4.98-6.44 kgf/mm 2 The haze and transmittance of the poly alpha-1,3-glucan ester film can be determined by methods well known in the art. As used herein, the term "haze" refers to the percentage of light that is deflected more than 2.5 degrees from the incoming light direction. Low haze values correspond to better clarity. The term 30 "transmittance" as used herein, refers to the fraction of incident light at a specified wavelength that passes through a film. The suitable film for the poly 22 WO 2015/094402 PCT/US2014/044281 alpha-1,3-glucan acetate film in this application can have a haze up to 20% and the transmittance of at least 80%. In an embodiment, the haze is 6.2% and the transmittance is 94.6%. The speed with which the film is produced can be increased by adding a 5 weak solvent, such as methanol and cyclohexane, ethanol and n-butanol or abundant methanol or ethanol in addition to methylene chloride, into the poly alpha-1,3-glucan ester solution to accelerate the solidification speed. One can restrain planar orientation degree and crystallization degree by controlling the surface temperature and shrinkage percentage of a film. 10 EXAMPLES The disclosed invention is further defined in the following Examples. It should be understood that these Examples, while indicating certain preferred aspects of the invention, are given by way of illustration only. From the above discussion and these Examples, one skilled in the art can ascertain the essential 15 characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various uses and conditions. Abbreviations: "mL" is milliliter(s); "g" is gram(s); "DI water" is deionized water; "tL" is 20 microliter(s); " C" is degrees Celsius; "mg" is milligram(s); "TFA" is trifluoroacetic acid; "Hz" is Hertz; "MHz" is mega Hertz; "ppm" is parts per million; "HFIP" is hexafluoro-2-propanol; "TFA-d" is deuterated trifluoroacetic acid, "kgf" is kilogram force. Materials 25 Sulfuric acid, acetic acid and sodium bicarbonate were from EMD Chemicals (Billerica, MA). Acetic anhydride was from Acros Organics (Pittsburgh, PA). Butyric acid, butyric anhydride, propionic anhydride and 0.1 N perchloric acid in acetic acid were from Sigma Aldrich (St. Louis, MO). Propionic acid was from JT Baker (Center Valley, PA). Magnesium acetate was from Alfa 30 Aesar (Ward Hill, MA). Unless otherwise specified, all acids and anhydrides used herein were water-free or substantially water-free. 23 WO 2015/094402 PCT/US2014/044281 Preparation of Poly Alpha-1,3-Glucan Poly alpha-1,3-glucan was prepared using a gtfJ enzyme preparation as described in U.S. Patent Appl. Publ. No. 2013/0244288, which is incorporated herein by reference in its entirety. 5 1 H Nuclear Maqnetic Resonance (NMR) Method for Determininq Degree of Substitution of Poly Alpha-1,3-Glucan Acetate Derivatives Degree of substitution (DoS) in poly alpha-1,3-glucan acetate ester derivatives was determined using 1 H NMR. Approximately 20 mg of derivative sample was weighed into a vial on an analytical balance. The vial was removed 10 from the balance and 0.7 mL of TFA-d was added to the vial. A magnetic stir bar was added to the vial and the mixture was stirred until the solid sample dissolved. Deuterated benzene (C 6

D

6 ), 0.3 mL, was then added to the vial to provide a better NMR lock signal than the TFA-d would provide. A portion, 0.8 mL, of the solution was transferred using a glass pipet into a 5-mm NMR tube. A 15 quantitative 1 H NMR spectrum was acquired using an Agilent VNMRS 400 MHz NMR spectrometer equipped with a 5-mm Autoswitchable Quad probe. The spectrum was acquired at a spectral frequency of 399.945 MHz using a spectral window of 6410.3 Hz, an acquisition time of 1.278 seconds, and an inter-pulse delay of 10 seconds and 124 pulses. The time domain data were transformed 20 using exponential multiplication of 0.78 Hz. Two regions of the resulting spectrum were integrated: from 3.1 ppm to 6.0 ppm, giving the integral for the seven poly alpha-1,3-glucan protons, and from 1.4 ppm to 2.7 ppm, giving the integral for the three acetyl protons. The degree of acetylation was calculated by dividing one third of the acetyl protons 25 integral area by one seventh of the poly alpha-1,3-glucan protons integral area. 1 H NMR Method for Determininq Degree of Substitution of Poly Alpha-1 3-Glucan Propionate Derivatives DoS in poly alpha-1,3-glucan propionate ester derivatives was determined using 1 H NMR. Approximately 20 mg of derivative sample was weighed into a 30 vial on an analytical balance. The vial was removed from the balance and 0.7 mL of TFA-d was added to the vial. A magnetic stir bar was added to the vial 24 WO 2015/094402 PCT/US2014/044281 and the mixture was stirred until the solid sample dissolved. Deuterated benzene

(C

6

D

6 ), 0.3 mL, was then added to the vial to provide a better NMR lock signal than the TFA-d would provide. A portion, 0.8 mL, of the solution was transferred using a glass pipet into a 5-mm NMR tube. A quantitative 1 H NMR spectrum was 5 acquired using an Agilent VNMRS 400 MHz NMR spectrometer equipped with a 5-mm Autoswitchable Quad probe. The spectrum was acquired at a spectral frequency is 399.945 MHz using a spectral window of 6410.3 Hz, an acquisition time of 1.278 seconds, and an inter-pulse delay of 10 seconds and 32 pulses. The time domain data were transformed using exponential line broadening of 1.0 10 Hz and the benzene solvent peak was set to 7.15 ppm. For poly alpha-1,3-glucan propionate samples, three regions of the resulting spectrum were integrated: from 3.3 ppm to 6.0 ppm, giving the integral for the seven poly alpha-1,3-glucan protons; from 1.9 ppm to 2.7 ppm, giving the integral for the methylene group of the propionyl group plus the methyl group of 15 the acetyl group; and from 0.8 ppm to 1.3 ppm, giving the integral for the methyl group of the propionyl group. The DoS by propionyl groups was calculated by dividing the integral value for the methyl group of the propionyl group by three. The integral value of the propionyl group's methylene group was then calculated by multiplying the integral 20 value for the methyl group of the propionyl group by 0.666. This value was then subtracted from the integral for the region of the methylene group of the propionyl group plus the methyl group of the acetyl group to give the integral value for the acetyl group's methyl group. 1 H NMR Method for Determininq Deqree of Substitution of Poly Alpha-1 3-Glucan 25 Mixed Ester Derivatives DoS in poly alpha-1,3-glucan mixed ester derivatives was determined using 1 H NMR. Approximately 20 mg of derivative sample was weighed into a vial on an analytical balance. The vial was removed from the balance and 0.7 mL of TFA-d was added to the vial. A magnetic stir bar was added to the vial 30 and the mixture was stirred until the solid sample dissolved. Deuterated benzene

(C

6

D

6 ), 0.3 mL, was then added to the vial to provide a better NMR lock signal 25 WO 2015/094402 PCT/US2014/044281 than the TFA-d would provide. A portion, 0.8 mL, of the solution was transferred using a glass pipet into a 5-mm NMR tube. A quantitative 1 H NMR spectrum was acquired using an Agilent VNMRS 400 MHz NMR spectrometer equipped with a 5-mm Autoswitchable Quad probe. The spectrum was acquired at a spectral 5 frequency of 399.945 MHz using a spectral window of 6410.3 Hz, an acquisition time of 1.278 seconds, and inter-pulse delay of 10 seconds and 32 pulses. The time domain data were transformed using exponential line broadening of 1.0 Hz and the benzene solvent peak was set to 7.15 ppm. For poly alpha-1,3-glucan acetate propionate samples, three regions of 10 the resulting spectrum were integrated: from 3.3 ppm to 6.0 ppm, giving the integral for the seven poly alpha-1,3-glucan protons; from 1.9 ppm to 2.7 ppm giving the integral for the methylene group of the propionyl group plus the methyl group of the acetyl group; and from 0.8 ppm to 1.3 ppm giving the integral for the methyl group of the propionyl group. 15 The DoS by propionyl groups on the glucan was calculated by dividing the integral value for the methyl group of the propionyl group by three. The integral value of the propionyl group's methylene group was then calculated by multiplying the integral value for the methyl group of the propionyl group by 0.666. This value was then subtracted from the integral for the region of the 20 methylene group of the propionyl group plus the methyl group of the acetyl group to give the integral value for the acetyl group's methyl group. Finally, the acetyl group integral value was divided by three to obtain the degree of acetylation. For poly alpha-1,3-glucan acetate butyrate samples, three regions of the resulting spectrum were integrated: from 3.3 ppm to 6.0 ppm giving the integral 25 for the seven poly alpha-1,3-glucan protons; from 1.9 ppm to 2.6 ppm giving the integral for the methylene group alpha to the carbonyl of the butyryl group plus the methyl group of the acetyl group; and from 0.6 ppm to 1.0 ppm giving the integral for the methyl group of the butyryl group. The DoS by butyryl groups on the glucan was calculated by dividing the 30 integral value for the methyl group of the butyryl group by three. The integral value of the butyryl group's methylene group was then calculated by multiplying 26 WO 2015/094402 PCT/US2014/044281 the integral value for the methyl group of the butyryl group by 0.666. This value was then subtracted from the integral for the region of the methylene group of the butyryl group plus the methyl group of the acetyl group to give the integral value for the acetyl group's methyl group. Finally, the acetyl group integral value was 5 divided by three to obtain the degree of acetylation. Determination of the Deqree of Polymerization The degree of polymerization (DP) was determined by size exclusion chromatography (SEC). Poly alpha-1,3-glucan ester was dissolved in HFIP (2 mg/mL) with shaking for 4 hours at 45 C. The chromatographic system used 10 was AllianceTM 2695 separation module from Waters Corporation (Milford, MA) coupled with three on-line detectors: a differential refractometer 2410 from Waters, a multi-angle light-scattering photometer HeleosTM 8+ from Wyatt Technologies (Santa Barbara, CA), and a differential capillary viscometer ViscoStarTM from Wyatt Technologies. The columns used for SEC were two 15 Shodex (Showa Denko America, New York) GPC HFIP-806M TM styrene-divinyl benzene columns and one Shodex GPC HFIP-804M TM styrene-divinyl benzene column. The mobile phase was redistilled HFIP with 0.01 M sodium trifluoroacetate. Chromatographic conditions used were 50 0 C at column and detector compartments, 40 0 C at sample and injector compartments, a flow rate 20 of 0.5 mL/min, and injection volume of 100 tL. Software packages used for data reduction were Astra version 6 from Wyatt (triple detection method with column calibration). EXAMPLE 1 Preparation of Acid-Exchanged Poly Alpha-1,3-Glucan 25 This Example describes producing acid-exchanged poly alpha-1,3-glucan, which can be used for preparing ester derivatives of poly alpha-1,3-glucan. Acid-exchanged poly alpha-1,3-glucan was prepared by placing 10 g of poly alpha-1,3-glucan in a 250-mL glass beaker with 150 mL of DI water. This mixture was boiled for one hour on a hot plate, afterwhich the poly alpha-1,3 30 glucan was recovered by vacuum filtration. The poly alpha-1,3-glucan was then subjected to two acid exchange steps of stirring it with 100 mL of glacial acetic 27 WO 2015/094402 PCT/US2014/044281 acid at room temperature followed by vacuum filtration, thereby providing acid exchanged poly alpha-1,3-glucan. Other forms of acid-exchanged poly alpha-1,3-glucan were also prepared by following the above process, but using propionic acid or butyric acid instead of 5 acetic acid. Acid-exchanged poly alpha-1,3-glucan prepared by these techniques was used in certain of the following examples to prepare various poly alpha-1,3 glucan ester derivatives. Since the acid exchange process removes water from the poly alpha-1,3-glucan, introduction of acid-exchanged poly alpha-1,3-glucan 10 to an esterification reaction with an acid anhydride does not introduce water which may react with the acid anhydride. EXAMPLE 2 Preparation of Poly Alpha-1,3-Glucan Acetate This Example describes producing the glucan ester derivative, poly alpha 15 1,3-glucan acetate. Acid-exchanged poly alpha-1,3-glucan (10 g) as prepared in Example 1 using acetic acid was added to a mixture containing 180 mL of acetic acid and 1.84 g of sulfuric acid in a 500-mL round bottom flask equipped with a magnetic stir bar, thermocouple and condenser. This mixture was stirred for 1 minute at 20 ambient temperature, afterwhich acetic anhydride (50 mL) was added to the mixture. The reaction was allowed to proceed for 30 minutes at ambient temperature, and then heated in a water bath at 35 C for 20 minutes followed by heating to 50 C for 30 minutes. The resulting reaction preparation did not contain any solids. The reaction was then removed from the water bath and 25 allowed to chill for 15 minutes to reach 42 OC. The reaction was then quenched with 25 mL of 70% acetic acid and stirred for 40 minutes. Poly alpha-1,3-glucan acetate was precipitated using an air-powered blender and DI water. The solid was washed twice with water for 30 minutes, followed by one wash with 5% sodium bicarbonate. The poly alpha-1,3-glucan acetate solid was then finally 30 washed with water until neutral pH was achieved (two water washes). The solid was collected by vacuum filtration, dried under vacuum, and characterized by 28 WO 2015/094402 PCT/US2014/044281 NMR and SEC. This method yielded poly alpha-1,3-glucan acetate with a DoS of 2.3 and an Mo of 29170. Thus, the ester derivative, poly alpha-1,3-glucan acetate, was prepared and isolated. 5 EXAMPLE3 Additional Preparation of Poly Alpha-1,3-Glucan Acetate This Example describes producing the glucan ester derivative, poly alpha 1,3-glucan acetate, using various reaction conditions. Acid-exchanged poly alpha-1,3-glucan was prepared as in Example 1 10 using acetic acid. A mixture of 180 mL acetic acid and 0.08 g of concentrated sulfuric acid was prepared in a 500-mL round bottom flask equipped with a magnetic stir bar and thermocouple; this mixture was chilled to 18 OC. The acid exchanged poly alpha-1,3-glucan (10 g) was slowly added to the chilled mixture and stirred for 1 minute. Acetic anhydride (50 mL) was then added to the 15 mixture. The reaction was allowed to proceed for 10 minutes with no heating, and then heated in a water bath at 35 0C for 20 minutes. The resulting reaction, which was devoid of any solid, was chilled to 22 0C over 7 minutes using an ice bath. The reaction was then quenched with 25 mL of 70% acetic acid and stirred for 40 minutes. Poly alpha-1,3-glucan acetate was precipitated, washed and 20 analyzed as described in Example 2. This process yielded poly alpha-1,3-glucan acetate with a DoS of 2.41 and an Mo of 73960. Using different concentrations of reagents allowed for different ester products to be formed. Table 1 below shows different poly alpha-1,3-glucan acetate esters synthesized using processes similar to the above process, but 25 with certain modifications as indicated in the table. The results in Table 1 indicate that by altering the reaction conditions and the molecular weight of poly alpha-1,3-glucan starting material used in the reaction, the DoS by acetyl groups in the ester product, as well as the molecular weight of the product, can be altered. 29 WO 2015/094402 PCT/US2014/044281 Table 1 Poly Alpha-1,3-Glucan Acetate Prepared from Poly Alpha-1,3-Glucan Poly alpha-1,3- Reaction Amount of acetic anhydride Poly alpha-1,3 glucan starting time and and sulfuric acid catalyst glucan acetate material temp". used in each reaction product Acetic Amount anhydride Sulfuric Acid Mn (g) 'C min (mL) ( Mn DoS 66K 10 36 40 50 0.9 73960 2.41 66K 10 30 45 50 1.6 78160 2.55 78K 50 47 35 84 1.8 58800 2.57 66K 10 36 30 50 0.8 48940 2.15 66K 10 32 45 50 0.8 87510 2.6 a Temperature after heating the reaction. 5 Thus, various forms of the ester derivative, poly alpha-1,3-glucan acetate, were prepared and isolated. EXAMPLE 4 Additional Preparation of Poly Alpha-1,3-Glucan Acetate This Example describes a process having a hydrolysis step for producing 10 poly alpha-1,3-glucan acetate. Acid-exchanged poly alpha-1,3-glucan (28 g) as prepared in Example 1 using acetic acid was added to a mixture containing 93.4 mL of acetic acid and 2.24 g of concentrated sulfuric acid and mixed. This mixture was added to a 1 -L jacketed reaction vessel equipped with an overhead stirrer and thermocouple, 15 and chilled to 12 C using a recirculating bath. The reaction mixture was then stirred for 1 minute before acetic anhydride (89 mL) was added. The reaction was heated using a recirculation bath set at 42 0C for 40 minutes. The reaction at this stage, which was devoid of any solid, was quenched with 15.25 g (24%) magnesium acetate with excess water to reduce sulfuric acid content to 2%. The 20 reaction was then heated to 100 C over 25 minutes, afterwhich it was stirred at this temperature for 2 hour. The reaction was completely quenched by adding 24% magnesium acetate in 5% excess (6.1 g). Poly alpha-1,3-glucan acetate was precipitated, washed and analyzed as in Example 2. This process yielded poly alpha-1,3-glucan acetate with a DoS of 2.58. 30 WO 2015/094402 PCT/US2014/044281 Thus, the ester derivative, poly alpha-1,3-glucan acetate, was prepared using a method incorporating a hydrolysis step. EXAMPLE 5 Preparation of Poly Alpha-1,3-Glucan Acetate Via Hydrolysis of Poly Alpha-1,3 5 Glucan Triacetate This Example describes, in part, preparing poly alpha-1,3-glucan acetate by hydrolysis of poly alpha-1,3-glucan triacetate. Poly alpha-1,3-glucan triacetate was first prepared as follows. Acetic acid (384 mL), acetic anhydride (990 mL), and methylene chloride 10 (890 mL) were mixed. This preparation was added to a 4-L glass reaction vessel equipped with an overhead stirrer and thermocouple, and chilled to 12 C. Acid exchanged poly alpha-1,3-glucan (130 g) as prepared in Example 1 using acetic acid was slowly added to the chilled mixture and stirred for 1 minute. Perchloric acid (0.1 N) in acetic acid (180 mL) was then added. The reaction was allowed 15 to proceed at ambient temperature for 3 hour and 35 minutes. The reaction, which was devoid of any solids, was then added to an air-powered blender containing methanol to precipitate poly alpha-1,3-glucan triacetate. The poly alpha-1,3-glucan triacetate solid thus formed was washed for 30 minutes with methanol followed by two washes with deionized (DI) water and one wash with 20 5% sodium bicarbonate. The poly alpha-1,3-glucan triacetate solid was then finally washed with water until neutral pH was achieved (two water washes) and collected by vacuum filtration, dried under vacuum, and characterized by NMR and SEC. The poly alpha-1,3-glucan triacetate produced had a DoS of 3.0 and an Mo of 132300. 25 The poly alpha-1,3-glucan triacetate produced above was set up for hydrolysis by first dissolving it in 80 mL of acetic acid. DI water (4 mL) was then added to this preparation and stirred using a magnetic stir bar until thoroughly mixed. The preparation was then transferred to a Parr Reactor (Parr Instrument Company, Moline, IL); steam at a pressure of 5 kg/cm 2 was blown into the 30 reactor to raise the temperature to 150 0 C in 12 minutes. The preparation was held at this temperature for 50 minutes. The pressure was then increased from 5 31 WO 2015/094402 PCT/US2014/044281 kg/cm 2 to 8.37 kg/cm 2 , afterwhich the reaction vessel was chilled to ambient temperature. The preparation retrieved from the reactor was yellow in color. However, upon addition of DI water, white solids of poly alpha-1,3-glucan acetate precipitated. This poly alpha-1,3-glucan acetate was isolated using vacuum 5 filtration, and washed and analyzed as in Example 2. This process yielded poly alpha-1,3-glucan acetate with a DoS of 2.4 and an Mo of 44200. Thus, poly alpha-1,3-glucan acetate with a DoS below 2.75 was prepared from poly alpha-1,3-glucan triacetate. EXAMPLE 6 10 Preparation of Poly Alpha-1,3-Glucan Propionate This Example describes producing the glucan ester derivative, poly alpha 1,3-glucan propionate. Acid-exchanged poly alpha-1,3-glucan (Mn of 119130) was prepared as described in Example 1 except that propionic acid was used instead of acetic 15 acid. Propionic acid (8 mL) and sulfuric acid (0.03 g) were mixed in a 250-mL round bottom flask and chilled to 18 C. The acid-exchanged poly alpha-1,3 glucan (2 g) was slowly added to the chilled mixture and stirred for 1 minute. Propionic anhydride (10 mL) was then added to this preparation, afterwhich 0.6 mL glacial acetic acid was added. The reaction was allowed to proceed for 5 20 minutes with no heating, and then heated in a water bath at 42 0C for 1 hour and 45 minutes. The maximum temperature was not allowed to go beyond 43 0C to avoid excess degradation of molecular weight. The resulting reaction preparation, which was devoid of any solids, was chilled to 20 0C using an ice bath over 5 minutes. The reaction was then quenched with 4 mL of 50% 25 aqueous acetic acid and stirred for 45 minutes. Poly alpha-1,3-glucan propionate was precipitated using an air-powered blender and DI water. The solid was washed twice with water for 30 minutes followed by a wash with 5% sodium bicarbonate. The poly alpha-1,3-glucan propionate solid was then washed with water until neutral pH was achieved (two water washes). The solid was collected 30 by vacuum filtration, dried under vacuum, and characterized by NMR and SEC. 32 WO 2015/094402 PCT/US2014/044281 The solid was confirmed as poly alpha-1,3-glucan propionate with 44.1 wt% propionyl groups (0 wt% acetyl groups) and an Mn of 59510. Thus, the ester derivative, poly alpha-1,3-glucan propionate, was prepared and isolated. 5 EXAMPLE7 Preparation of Poly Alpha-1,3-Glucan Acetate Butyrate This Example describes producing the glucan mixed ester derivative, poly alpha-1,3-glucan acetate butyrate. Acid-exchanged poly alpha-1,3-glucan (10 g) as prepared in Example 1 10 with acetic acid was added to a mixture containing 21 mL of glacial acetic acid, 20 mL of butyric acid and 0.09 g of sulfuric acid in a 500-mL round bottom flask equipped with a magnetic stir bar, thermocouple and condenser. The mixture was chilled to 18 0C using an ice bath and stirred for 1 minute before butyric anhydride (39 mL) was added to the flask. The reaction was allowed to proceed 15 for 10 minutes with no heating, and then heated in a water bath at 35 C for 80 minutes, followed by heating to 39 C for 30 minutes where the maximum temperature reached was 39 0C to avoid excess degradation of product molecular weight. The resulting viscous solution, which was devoid of any solids, was cooled to 20 C using an ice bath for 10 minutes. The reaction was then 20 quenched with 20 mL of 50% aqueous acetic acid and stirred for 40 minutes. Solid poly alpha-1,3-glucan acetate butyrate was precipitated using an air powered blender and DI water. The solid was washed twice with water for 30 minutes, followed by washing with 5% sodium bicarbonate. The solid thus obtained was then finally washed with DI water until neutral pH was achieved 25 (two water washes). The solid was collected by vacuum filtration, dried under vacuum, and characterized by NMR and SEC. This process yielded poly alpha 1,3-glucan acetate butyrate mixed ester with a butyryl DoS of 1.0, an acetyl DoS of 1.3, and a number-average molecular weight (Mo) of 66340. Using different concentrations of reagents allowed for different mixed ester 30 products to be formed. Table 2 below shows different poly alpha-1,3-glucan acetate butyrate esters synthesized using processes similar to the above 33 WO 2015/094402 PCT/US2014/044281 process, but with certain modifications as indicated in the table. The results in Table 2 indicate that by altering the reaction conditions and the molecular weight of poly alpha-1,3-glucan starting material used in the reaction, the amount of acetyl and butyryl groups in the mixed ester product, as well as the molecular 5 weight of the product, can be altered. 34 WO 2015/094402 PCT/US2014/044281 CO I- COC\ o0 cco __o oo :3 co -C -0 CC I~ - m LO 000 co LO (. 0 0C m I ~ - LO ~ 0 Z~ E a) c~ .2 co _ 4. 0 a) .c _ _o co co Co D- ". -:5 >, UW 0 0 _0 =~0~ co c 7 4-

-

U 0 0 E co <Coa, c co -00 0Co a)a, L. 0 ) -o a) co A~ 0 0)CJ\ C~ C

-C

coo~ E C) C) WO 2015/094402 PCT/US2014/044281 Thus, various forms of the mixed ester derivative, poly alpha-1,3-glucan acetate butyrate, were prepared and isolated. EXAMPLE 8 Preparation of Poly Alpha-1,3-Glucan Acetate Propionate 5 This Example describes producing the glucan mixed ester derivative, poly alpha-1,3-glucan acetate propionate. Acid-exchanged poly alpha-1,3-glucan was prepared as described in Example 1 using acetic acid. A mixture of 35 mL of propionic acid and 0.09 g sulfuric acid was prepared in a 500-mL round bottom flask and chilled to 18 OC. 10 Acid-exchanged poly alpha-1,3-glucan solid (10 g) was slowly added to the chilled mixture and stirred for 1 minute. Propionic anhydride (50 mL) was then added, afterwhich 5 mL of glacial acetic acid was added. The reaction was allowed to proceed for 10 minutes with no heating, and then heated in a water bath at 30 0C for 1 hour, followed by heating to 34 0C for 10 minutes. The 15 maximum temperature was not allowed to go beyond 36 0C to avoid excess degradation of product molecular weight. The solution thus obtained, which was devoid of any solids, was chilled to 20 0C in an ice bath for 5 minutes. The reaction was then quenched with 20 mL of 50% aqueous acetic acid and stirred for 40 minutes. Poly alpha-1,3-glucan acetate propionate was precipitated from 20 the solution using an air-powered blender and DI water. The solid poly alpha 1,3-glucan acetate propionate product was washed twice with water for 30 minutes followed by a wash with 5% sodium bicarbonate. The solid was then washed with water until neutral pH was achieved (two water washes). The solid was collected by vacuum filtration, dried under vacuum, and characterized by 25 NMR and SEC. The solid created was confirmed as a poly alpha-1,3-glucan acetate propionate containing 17.6 wt% acetyl and 32.9 wt% propionyl groups and having an Mo of 64030. Using different concentrations of reagents allowed for different mixed ester products to be formed. Table 3 below shows different poly alpha-1,3-glucan 30 acetate propionate esters synthesized using processes similar to the above process, but with certain modifications as indicated in the table. The results in 36 WO 2015/094402 PCT/US2014/044281 Table 3 indicate that by altering the reaction conditions and the molecular weight of poly alpha-1,3-glucan starting material used in the reaction, the amount of acetyl and propionyl groups in the mixed ester product, as well as the molecular weight of the product, can be altered. 37 WO 2015/094402 PCT/US2014/044281 0 CO__ C-D c0 '0 A C: Z - - M r- -00 2- a) CD CDCD CD CD CD a) -t caO Cc - 0 < w w MZCD MLO 0 0o C 0 UO C)ro L 0 00 LU) 2~c - - - 00) 0C) U U)) C:) .C4- 0 - L O:) c HOO (n< crU) a) 4C 0 j C:) C:) m Lo c V -0 co 0 0 oc D) 0-C~ - o 2 Co2 __ 0 000) r)00 00 a r a 7U)0 < 0- .0 .00 -0 ~\ 0 cr) cc 0 )0 co~~ ~ 0 a)C oML CDC: CD Ico~ C < < (3) cz f _0 70 0 ) WO 2015/094402 PCT/US2014/044281 Thus, various forms of the mixed ester derivative, poly alpha-1,3-glucan acetate propionate, were prepared and isolated. EXAMPLE 9 Preparation of Poly Alpha-1,3-Glucan Triacetate Usinq Sulfuric Acid as Catalyst 5 This Example describes producing poly alpha-1,3-glucan triacetate using sulfuric acid as the catalyst in the reaction. Acid-exchanged poly alpha-1,3-glucan was prepared as described in Example 1 using acetic acid. A mixture of 180 mL acetic acid and 1.84 g of concentrated sulfuric acid, as a catalyst, was prepared in a 500-mL round bottom 10 flask equipped with an overhead stirrer and thermocouple. Acid-exchanged poly alpha-1,3-glucan (10 g) was slowly added to the mixture and stirred under nitrogen for 1 minute. This mixture was chilled to about 18 0C using an ice bath. Acetic anhydride (50 mL) was added to the reaction, which was then heated to 80 0C over 45 minutes and allowed to react at this temperature for 30 minutes. 15 The reaction, which was devoid of any solids, was chilled to 40 C using an ice bath over 5 minutes. The reaction was then quenched with 25 mL of 70% acetic acid and stirred for 30 minutes. Poly alpha-1,3-glucan triacetate was precipitated using an air-powered blender (Waring, Torrington, CT) and DI water. The solid poly alpha-1,3-glucan triacetate product was washed with water for 30 minutes 20 twice, followed by washing with 5% sodium bicarbonate. The solid was then washed with water until neutral pH was achieved (two water washes). The solid was collected by vacuum filtration, dried under vacuum, and characterized by NMR and SEC. This process yielded 7.8 g of poly alpha-1,3-glucan triacetate with a DoS of 3.1 and an Mn of 5130. The DoS reading over 3.0 likely reflects 25 integration variability typical to the NMR measurement process. Table 4 below shows different molecular weight poly alpha-1,3-glucan triacetate esters synthesized using processes similar to the above process, but with certain modifications as indicated in the table. The results in Table 4 indicate that by altering the reaction conditions and the molecular weight of poly 30 alpha-1,3-glucan starting material used in the reaction, the molecular weight of the product can be altered. 39 WO 2015/094402 PCT/US2014/044281 Table 4 Poly Alpha-1,3-Glucan Triacetate Esters Prepared from Poly Alpha-1,3-Glucan Using Sulfuric Acid Catalyst Poly alpha-1,3- Reaction Poly alpha-1,3 glucan starting time and Sulfuric glucan triacetate material tempa. Acid product Amount (wt% of Mn (g) 'C min glucan) Mn DoS 112K 10 43 40 8 68090 2.75 66K 10 59 60 8 29380 2.94 66K 28 39 55 8 n/a 2.91 a Maximum temperature after heating the reaction. 5 Thus, various forms of poly alpha-1,3-glucan triacetate were prepared and isolated in reactions using sulfuric acid as a catalyst. EXAMPLE 10 Preparation of Poly Alpha-1,3-Glucan Triacetate Using Perchloric Acid as 10 Catalyst This Example describes producing poly alpha-1,3-glucan triacetate using perchloric acid as the catalyst in the reaction. Acid-exchanged poly alpha-1,3-glucan was prepared as described in Example 1 using acetic acid. A mixture of 384 mL acetic acid, 990 mL acetic 15 anhydride, and 890 mL methylene chloride was prepared in a 4-L glass reaction vessel equipped with an overhead stirrer and thermocouple, and chilled to 12 OC. Acid-exchanged poly alpha-1,3-glucan (130 g) was slowly added to the chilled mixture and stirred for 1 minute. Perchloric acid (0.1 N) in acetic acid (180 mL) was then added to the mixture. The reaction was allowed to proceed at ambient 20 temperature for 3 hour and 35 minutes. The resulting reaction, which was devoid of solid, was added to an air-powered blender containing methanol to precipitate poly alpha-1,3-glucan triacetate. The poly alpha-1,3-glucan triacetate solid was washed for 30 minutes with methanol followed by washing twice with DI water and one wash with 5% sodium bicarbonate. The poly alpha-1,3-glucan triacetate 25 was then washed with water until neutral pH was achieved (two water washes). The solid was collected by vacuum filtration, dried under vacuum, and 40 WO 2015/094402 PCT/US2014/044281 characterized by NMR and SEC. This process produced 221.5 g of poly alpha 1,3-glucan triacetate with a DoS of 3.2 and an Mo of 132300. The DoS reading over 3.0 likely reflects integration variability typical to the NMR measurement process. 5 Table 5 below shows different molecular weight poly alpha-1,3-glucan triacetate esters synthesized using processes similar to the above process, but with certain modifications as indicated in the table. The results in Table 5 indicate that by altering the reaction conditions and the molecular weight of poly alpha-1,3-glucan starting material used in the reaction, the molecular weight of 10 the product can be altered. Table 5 Poly Alpha-1,3-Glucan Triacetate Esters Prepared from Poly Alpha-1,3-Glucan Using Perchloric Acid Catalyst Poly alpha-1,3- Reaction Poly alpha-1,3 glucan starting time and glucan triacetate material tempa. Perchloric product Amount Acid Mn (g) 'C min (g) Mn DoS 126K 130 32 180 1.80 132300 3.0 82K 30 30 270 0.49 130700 3.0 66K 130 41 150 2.17 93510 2.75 98K 130 37 215 1.80 197000 3.0 a Maximum temperature after heating the reaction. 15 Thus, various forms of poly alpha-1,3-glucan triacetate were prepared and isolated in reactions using perchloric acid as a catalyst. EXAMPLE 11 Preparation of Films Using Poly Alpha-1,3-Glucan Acetate 20 Poly alpha-1,3-glucan acetate, prepared as in Example 2, was dissolved in acetone at 10 wt% mixture to make a solution. The solution was then cast onto a clean glass plate with a film caster and the solvent was allowed to evaporate to dryness to provide a film. The film was removed from the glass and rinsed with DI water. Table 6 shows properties of two different poly alpha-1,3 25 glucan acetate films prepared using two different samples of poly alpha-1,3 glucan acetate. 41 WO 2015/094402 PCT/US2014/044281 Table 6 Poly Alpha-1,3-Glucan Acetate Films Poly Poly alpha- alpha-1,3 1,3-glucan glucan Breaking Tear Average acetate acetate Stress (gf/ Tear Thickness DoS Mn (kgf/mm 2 ) mil (microns) 2.6 87510 3.97 2.4 5.6 58.4 2.74 57240 4.81 2.0 6.8 86.4 EXAMPLE 12 5 Optical Analysis of Poly Alpha-1,3-Glucan Acetate Films A sample of poly alpha-1,3-glucan acetate film, prepared as in Example 11, was analyzed for color and haze. The spectra that were collected were consistent with ASTM El 164-09a. Spectral Bandwidth (SBW) = 1, at 1 nm interval and wavelength range = 830 - 360 nm. Table 7 shows the results of this 10 study. Table 7 Optical Measurements of a Poly Alpha-1,3-Glucan Acetate Film Poly alpha- Poly alpha 1,3-glucan 1,3-glucan acetate acetate DoS Mn Haze Transmittance 2.6 78160 6.32% 5.36% EXAMPLE 13 15 Preparation of Poly Alpha-1,3-Glucan Triacetate Films Poly alpha-1,3-glucan triacetate was prepared as described in Example 10. A 10 wt% solution of the poly alpha-1,3-glucan triacetate was prepared by dissolving 10 g of it in 90 g methylene chloride:methanol (11.5:1 v/v). This solution was then cast onto a clean glass plate with a Gardner Knife (Gardner 20 Lab Inc., Bethesda, MD). The solvent was allowed to evaporate to dryness. The film produced following solvent evaporation was removed from the glass and rinsed with DI water. Table 8 summarizes the tensile and tear data for poly 42 WO 2015/094402 PCT/US2014/044281 alpha-1,3-glucan triacetate films produced using this method. It can be seen that variation of the Ma and DoS of the constituent glucan ester results in providing different physical properties for the film produced. Table 8 5 Poly Alpha-1,3-Glucan Triacetate Films Poly Poly Breaking alpha-1,3- alpha-1,3- stress glucan glucan (kgf/mm 2 ) Tear/ Average triacetate triacetate Thickness Tear Thickness DoS Mn (gf/mil) (gf) (micrometers) 2.75 93510 4.98 1.4 6.9 129.5 3.0 93670 6.44 2.9 11.6 101.6 3.0 132300 5.9 2.5 14.8 152.4 3.0 197000 5.26 3.1 18.1 147.5 EXAMPLE 14 Thermal Analysis of Poly Alpha-1,3-Glucan Triacetate Films Poly alpha-1,3-glucan triacetate films as prepared in Example 13 were 10 analyzed using MDSC and TGA. MDSC measurements were performed with 5-6 mg of film at a heating rate of 3 0 C/min, modulation amplitude of 0.48 0C, and modulation period of 60 seconds starting from 0 C, in N 2 using Q1000 TA instrument. TGA experiments were performed from ambient temperature to 800 0C 15 under N 2 using Q500 TA instrument. The information provided in Table 9 shows the heat stability/thermal degradation of the poly alpha-1,3-glucan triacetate films prepared according to the method disclosed above. Table 9 summarizes data acquired from the MDSC 43 WO 2015/094402 PCT/US2014/044281 and TGA measurements. It can be seen that variation of the Mn and DoS of the constituent glucan ester results in providing different physical properties for the film produced. Table 9 5 MDSC and TGA Data for Poly Alpha-1,3-Glucan Triacetate Films Tm ('C), Tm ('C), Poly alpha- AH (J/g) AH (J/g) Onset of 1,3-glucan Tg (SC) Total Non-Rev Heat Decomposition triacetate (DoS/Mn) (Reverse) Heat flow flow (C) 333.0 332.9 2.75/93510 188.0 29.6 16.8 349.3 340.5 339.9 3.0/197000 189.1 39.4 29.6 356.7 EXAMPLE 15 Optical Analysis of Poly Alpha-1,3-Glucan Triacetate Films Poly alpha-1,3-glucan triacetate films prepared as in Example 13 were 10 analyzed for color and haze. Spectra were collected consistent with ASTM El 164-09a using spectral Bandwidth (SBW) of 1, at 1 nm interval and wavelength range of 830 - 360 nm. Results of optical measurements of poly alpha-1,3-glucan triacetate films are shown in Table 10. 44 WO 2015/094402 PCT/US2014/044281 Table 10 Optical Measurements of Poly Alipha-1,3-Glucan Triacetate Films Poly alpha- Poly alpha 1,3-glucan 1,3-glucan triacetate triacetate Haze Transmittance DoS Mn(%) (%) 3.0 130700 6.32 91.6 2.75 93510 1.41 92.1 53690 2.72 1.37 92.3 45

Claims (7)

1. A film comprising poly alpha-1,3-glucan ester having at least one of: (a) a tear resistance of at least about 0.1 gf/mil; or 5 (b) a haze of less than about 20%.

2. The film of claim 1, wherein the poly alpha-1,3-glucan ester used is poly alpha-1,3-glucan acetate. 10

3. The film of claim 1, wherein the film has at least one of: (a) a tear resistance from about 2.0 to about 2.4 gf/mil; or (b) a haze of less than about 10%.

4. The film of claim 3, wherein the poly alpha-1,3-glucan ester used is poly 15 alpha-1,3-glucan acetate.

5. A method to prepare a poly alpha-1,3-glucan ester film comprising: (a) providing poly alpha-1,3-glucan ester; (b) contacting the poly alpha-1,3-glucan ester of (a) with a solvent 20 to make a solution of poly alpha-1,3-glucan ester; (c) applying the solution of poly alpha-1,3-glucan ester on a surface; and (d) allowing the solvent to evaporate to provide the poly alpha-1,3 glucan ester film. 25

6. The method of claim 5, wherein the poly alpha-1,3-glucan ester used is poly alpha-1,3-glucan acetate.

7. The method of claim 5, wherein the solvent is acetone. 46