AU2014255759B2 - Yeast alleles involved in maximal alcohol accumulation capacity and tolerance to high alcohol levels - Google Patents
Yeast alleles involved in maximal alcohol accumulation capacity and tolerance to high alcohol levels Download PDFInfo
- Publication number
- AU2014255759B2 AU2014255759B2 AU2014255759A AU2014255759A AU2014255759B2 AU 2014255759 B2 AU2014255759 B2 AU 2014255759B2 AU 2014255759 A AU2014255759 A AU 2014255759A AU 2014255759 A AU2014255759 A AU 2014255759A AU 2014255759 B2 AU2014255759 B2 AU 2014255759B2
- Authority
- AU
- Australia
- Prior art keywords
- ethanol
- tolerance
- allele
- seg5
- pool
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 892
- 108700028369 Alleles Proteins 0.000 title claims abstract description 110
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 94
- 238000009825 accumulation Methods 0.000 title claims abstract description 83
- 101100297714 Arabidopsis thaliana PIX13 gene Proteins 0.000 claims abstract description 54
- 101100453925 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) KIN3 gene Proteins 0.000 claims abstract description 54
- 101100453926 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) KIN4 gene Proteins 0.000 claims abstract description 54
- 101100317188 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) VPS70 gene Proteins 0.000 claims abstract description 15
- 101150118846 MKT1 gene Proteins 0.000 claims description 10
- 101100323459 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) APJ1 gene Proteins 0.000 claims description 10
- 241000235070 Saccharomyces Species 0.000 claims description 6
- 108020004707 nucleic acids Proteins 0.000 claims description 6
- 102000039446 nucleic acids Human genes 0.000 claims description 6
- 150000007523 nucleic acids Chemical class 0.000 claims description 6
- 238000010276 construction Methods 0.000 abstract description 4
- 239000003550 marker Substances 0.000 description 93
- 108090000623 proteins and genes Proteins 0.000 description 86
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 84
- 238000000855 fermentation Methods 0.000 description 69
- 230000004151 fermentation Effects 0.000 description 69
- 238000000034 method Methods 0.000 description 48
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 38
- 239000008103 glucose Substances 0.000 description 38
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 36
- 238000004519 manufacturing process Methods 0.000 description 34
- 230000004663 cell proliferation Effects 0.000 description 33
- 238000004458 analytical method Methods 0.000 description 32
- 210000000349 chromosome Anatomy 0.000 description 31
- 230000012010 growth Effects 0.000 description 24
- 210000004027 cell Anatomy 0.000 description 22
- 230000002068 genetic effect Effects 0.000 description 21
- 101150050575 URA3 gene Proteins 0.000 description 20
- 238000013507 mapping Methods 0.000 description 18
- 101100246753 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) pyrF gene Proteins 0.000 description 17
- 108020004414 DNA Proteins 0.000 description 15
- 235000014101 wine Nutrition 0.000 description 15
- 238000012217 deletion Methods 0.000 description 14
- 230000037430 deletion Effects 0.000 description 14
- 230000000694 effects Effects 0.000 description 14
- 238000009826 distribution Methods 0.000 description 13
- 238000003556 assay Methods 0.000 description 12
- 239000007787 solid Substances 0.000 description 12
- 230000035772 mutation Effects 0.000 description 11
- 230000006798 recombination Effects 0.000 description 11
- 238000005215 recombination Methods 0.000 description 11
- 238000012300 Sequence Analysis Methods 0.000 description 10
- 238000003752 polymerase chain reaction Methods 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 230000007704 transition Effects 0.000 description 10
- 108091026890 Coding region Proteins 0.000 description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 240000000111 Saccharum officinarum Species 0.000 description 9
- 235000007201 Saccharum officinarum Nutrition 0.000 description 9
- 230000001965 increasing effect Effects 0.000 description 9
- 230000007935 neutral effect Effects 0.000 description 9
- 238000012163 sequencing technique Methods 0.000 description 9
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 8
- 230000035945 sensitivity Effects 0.000 description 8
- 238000013179 statistical model Methods 0.000 description 8
- 235000013405 beer Nutrition 0.000 description 7
- 230000000875 corresponding effect Effects 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 6
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 6
- 238000004422 calculation algorithm Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000002773 nucleotide Substances 0.000 description 6
- 125000003729 nucleotide group Chemical group 0.000 description 6
- 238000012070 whole genome sequencing analysis Methods 0.000 description 6
- 108700018454 CDC15 Proteins 0.000 description 5
- 101100188686 Danio rerio opn1sw2 gene Proteins 0.000 description 5
- 102100031414 EF-hand domain-containing protein D1 Human genes 0.000 description 5
- 101150096151 EFHD1 gene Proteins 0.000 description 5
- 101150089053 SWS2 gene Proteins 0.000 description 5
- 101100382067 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) BUD14 gene Proteins 0.000 description 5
- 101150081467 cdc15 gene Proteins 0.000 description 5
- 230000002759 chromosomal effect Effects 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 238000009655 industrial fermentation Methods 0.000 description 5
- 230000013011 mating Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 230000003252 repetitive effect Effects 0.000 description 5
- 230000000717 retained effect Effects 0.000 description 5
- 238000005204 segregation Methods 0.000 description 5
- 238000010200 validation analysis Methods 0.000 description 5
- 230000035899 viability Effects 0.000 description 5
- 238000007400 DNA extraction Methods 0.000 description 4
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 4
- 238000013459 approach Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000002224 dissection Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- BDERNNFJNOPAEC-UHFFFAOYSA-N n-propyl alcohol Natural products CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000028070 sporulation Effects 0.000 description 4
- -1 ADE1 Proteins 0.000 description 3
- 101100494773 Caenorhabditis elegans ctl-2 gene Proteins 0.000 description 3
- 101100112369 Fasciola hepatica Cat-1 gene Proteins 0.000 description 3
- 230000005526 G1 to G0 transition Effects 0.000 description 3
- 102100036263 Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial Human genes 0.000 description 3
- 101001001786 Homo sapiens Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial Proteins 0.000 description 3
- 101100005271 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cat-1 gene Proteins 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 241001123228 Saccharomyces paradoxus Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 235000013334 alcoholic beverage Nutrition 0.000 description 3
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 3
- 238000000876 binomial test Methods 0.000 description 3
- 230000003833 cell viability Effects 0.000 description 3
- 235000019993 champagne Nutrition 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000001186 cumulative effect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 238000012268 genome sequencing Methods 0.000 description 3
- 238000009499 grossing Methods 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000002018 overexpression Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 238000007480 sanger sequencing Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 210000005253 yeast cell Anatomy 0.000 description 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 101150007665 APJ1 gene Proteins 0.000 description 2
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 description 2
- 108700023418 Amidases Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 240000008168 Ficus benjamina Species 0.000 description 2
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 2
- 102000010029 Homer Scaffolding Proteins Human genes 0.000 description 2
- 101001077298 Homo sapiens Retinoblastoma-binding protein 5 Proteins 0.000 description 2
- 101100433290 Homo sapiens ZNF471 gene Proteins 0.000 description 2
- 101000615763 Homo sapiens tRNA-splicing endonuclease subunit Sen34 Proteins 0.000 description 2
- 108091092195 Intron Proteins 0.000 description 2
- 229910009891 LiAc Inorganic materials 0.000 description 2
- 240000003183 Manihot esculenta Species 0.000 description 2
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 2
- IMTUWVJPCQPJEE-IUCAKERBSA-N Met-Lys Chemical compound CSCC[C@H](N)C(=O)N[C@H](C(O)=O)CCCCN IMTUWVJPCQPJEE-IUCAKERBSA-N 0.000 description 2
- 101150034336 NUP60 gene Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 102000001253 Protein Kinase Human genes 0.000 description 2
- 102100025192 Retinoblastoma-binding protein 5 Human genes 0.000 description 2
- 101100389697 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ERP1 gene Proteins 0.000 description 2
- 101100033865 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) RFA1 gene Proteins 0.000 description 2
- 241000235343 Saccharomycetales Species 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 241000219094 Vitaceae Species 0.000 description 2
- 101100066419 Xenopus laevis fbxo43 gene Proteins 0.000 description 2
- 102100029037 Zinc finger protein 471 Human genes 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 238000001516 cell proliferation assay Methods 0.000 description 2
- 235000020057 cognac Nutrition 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 235000021021 grapes Nutrition 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000021121 meiosis Effects 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 102000054765 polymorphisms of proteins Human genes 0.000 description 2
- 108060006633 protein kinase Proteins 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- CJBPIZBHRWDBGQ-COSFPPCYSA-N rfa-1 Chemical compound C1([C@H]2N[C@H](CC3(N=C4C=5C6=C7O[C@](C6=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@@H](C)[C@@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC(=C4N3)C(=O)C=5C(O)=C7C)C)OC)C2)C=2C=CC=CC=2)=CC=CC=C1 CJBPIZBHRWDBGQ-COSFPPCYSA-N 0.000 description 2
- 238000013207 serial dilution Methods 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 102100021776 tRNA-splicing endonuclease subunit Sen34 Human genes 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 230000032258 transport Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 235000013522 vodka Nutrition 0.000 description 2
- 239000007222 ypd medium Substances 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 101150052453 ADE1 gene Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 102000016560 Aquaglyceroporins Human genes 0.000 description 1
- 108010092667 Aquaglyceroporins Proteins 0.000 description 1
- 101100520819 Arabidopsis thaliana At5g66631 gene Proteins 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 108010023063 Bacto-peptone Proteins 0.000 description 1
- 241000722885 Brettanomyces Species 0.000 description 1
- 244000027711 Brettanomyces bruxellensis Species 0.000 description 1
- 235000000287 Brettanomyces bruxellensis Nutrition 0.000 description 1
- 101100385390 Caenorhabditis elegans kin-3 gene Proteins 0.000 description 1
- 101100184274 Candida albicans (strain SC5314 / ATCC MYA-2876) MNL1 gene Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000005572 Cathepsin A Human genes 0.000 description 1
- 108010059081 Cathepsin A Proteins 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 241001559589 Cullen Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108020005124 DNA Adducts Proteins 0.000 description 1
- 230000005971 DNA damage repair Effects 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 102100030011 Endoribonuclease Human genes 0.000 description 1
- 101710199605 Endoribonuclease Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 1
- 244000286779 Hansenula anomala Species 0.000 description 1
- MMFKFJORZBJVNF-UWVGGRQHSA-N His-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CN=CN1 MMFKFJORZBJVNF-UWVGGRQHSA-N 0.000 description 1
- 108010077223 Homer Scaffolding Proteins Proteins 0.000 description 1
- 101150028077 KIN3 gene Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 108010006519 Molecular Chaperones Proteins 0.000 description 1
- 102000005431 Molecular Chaperones Human genes 0.000 description 1
- 108010029888 NIMA-Related Kinases Proteins 0.000 description 1
- 102000001783 NIMA-Related Kinases Human genes 0.000 description 1
- 238000004497 NIR spectroscopy Methods 0.000 description 1
- 241001327682 Oncorhynchus mykiss irideus Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 208000020584 Polyploidy Diseases 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- 101100078102 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) MSN2 gene Proteins 0.000 description 1
- 101100266439 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) TY1A-A gene Proteins 0.000 description 1
- 241000452111 Saccharomyces cerevisiae Kyokai no. 7 Species 0.000 description 1
- 244000253724 Saccharomyces cerevisiae S288c Species 0.000 description 1
- 235000004905 Saccharomyces cerevisiae S288c Nutrition 0.000 description 1
- 241000277263 Salmo Species 0.000 description 1
- 241001633332 Scheffersomyces stipitis CBS 6054 Species 0.000 description 1
- 101710113029 Serine/threonine-protein kinase Proteins 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 241000009298 Trigla lyra Species 0.000 description 1
- 241000235033 Zygosaccharomyces rouxii Species 0.000 description 1
- 230000001594 aberrant effect Effects 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 150000008431 aliphatic amides Chemical class 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 102000005922 amidase Human genes 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000003322 aneuploid effect Effects 0.000 description 1
- 208000036878 aneuploidy Diseases 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000003766 bioinformatics method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000002230 centromere Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000028564 filamentous growth Effects 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 108091008053 gene clusters Proteins 0.000 description 1
- 238000012224 gene deletion Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000011331 genomic analysis Methods 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 108010025306 histidylleucine Proteins 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000005865 ionizing radiation Effects 0.000 description 1
- 235000015095 lager Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004890 malting Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000007481 next generation sequencing Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000001503 one-tailed test Methods 0.000 description 1
- 238000009401 outcrossing Methods 0.000 description 1
- 238000007427 paired t-test Methods 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- JTJMJGYZQZDUJJ-UHFFFAOYSA-N phencyclidine Chemical compound C1CCCCN1C1(C=2C=CC=CC=2)CCCCC1 JTJMJGYZQZDUJJ-UHFFFAOYSA-N 0.000 description 1
- 239000003016 pheromone Substances 0.000 description 1
- RCIMBBZXSXFZBV-UHFFFAOYSA-N piromidic acid Chemical compound N1=C2N(CC)C=C(C(O)=O)C(=O)C2=CN=C1N1CCCC1 RCIMBBZXSXFZBV-UHFFFAOYSA-N 0.000 description 1
- 238000012068 polygenic analysis Methods 0.000 description 1
- 230000003234 polygenic effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000011176 pooling Methods 0.000 description 1
- 238000012913 prioritisation Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000000135 prohibitive effect Effects 0.000 description 1
- 230000025220 protein targeting to vacuole Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000002708 random mutagenesis Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000008458 response to injury Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 229940126586 small molecule drug Drugs 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000000528 statistical test Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 102000055501 telomere Human genes 0.000 description 1
- 108091035539 telomere Proteins 0.000 description 1
- 210000003411 telomere Anatomy 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
- 238000012049 whole transcriptome sequencing Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP13163727 | 2013-04-15 | ||
EP13163727.4 | 2013-04-15 | ||
PCT/EP2014/057629 WO2014170330A2 (en) | 2013-04-15 | 2014-04-15 | Yeast alleles involved in maximal alcohol accumulation capacity and tolerance to high alcohol levels |
Publications (2)
Publication Number | Publication Date |
---|---|
AU2014255759A1 AU2014255759A1 (en) | 2015-11-05 |
AU2014255759B2 true AU2014255759B2 (en) | 2019-10-03 |
Family
ID=48083055
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2014255759A Ceased AU2014255759B2 (en) | 2013-04-15 | 2014-04-15 | Yeast alleles involved in maximal alcohol accumulation capacity and tolerance to high alcohol levels |
Country Status (6)
Country | Link |
---|---|
US (1) | US10000759B2 (de) |
EP (1) | EP2986707A2 (de) |
AU (1) | AU2014255759B2 (de) |
BR (1) | BR112015026063A2 (de) |
CA (1) | CA2909503A1 (de) |
WO (1) | WO2014170330A2 (de) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK2900690T3 (da) | 2012-09-25 | 2019-10-21 | Vib Vzw | Mutant gærstamme med reduceret glycerolproduktion |
WO2014170330A2 (en) | 2013-04-15 | 2014-10-23 | Vib Vzw | Yeast alleles involved in maximal alcohol accumulation capacity and tolerance to high alcohol levels |
WO2017037241A1 (en) * | 2015-09-03 | 2017-03-09 | Vib Vzw | Yeast alleles involved in tolerance to high alcohol levels |
CN108368475A (zh) | 2015-11-25 | 2018-08-03 | 拉金德拉·苏拉纳 | 通过经改良酵母菌株酿酒酵母在高温下生产高量乙醇的方法 |
WO2018115251A1 (en) | 2016-12-21 | 2018-06-28 | Vib Vzw | Xylose isomerases that confer efficient xylose fermentation capability to yeast |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1338608A3 (de) | 2001-12-20 | 2004-05-06 | Cellzome Ag | Protein-Komplexe und deren Verwendung |
DE60325457D1 (de) | 2002-01-23 | 2009-02-05 | Royal Nedalco B V | Fermentation von pentosezuckern |
US20100317078A1 (en) | 2007-08-27 | 2010-12-16 | Cornell Research Foundation Inc | Methods to improve alcohol tolerance of microorganisms |
US8865411B2 (en) * | 2009-03-26 | 2014-10-21 | National Institutes Of Health (Nih) | Methods of identifying modulators of TDP-43 mediated cellular toxicity |
US20100311137A1 (en) | 2009-06-08 | 2010-12-09 | Ut-Battelle, Llc | Microorganisms Having Enhanced Tolerance To Inhibitors and Stress |
US20140228224A1 (en) | 2011-06-21 | 2014-08-14 | Katholieke Universiteit Leuven, K.U.Leuven R&D | Specific alleles important for ethanol tolerance |
DK2900690T3 (da) | 2012-09-25 | 2019-10-21 | Vib Vzw | Mutant gærstamme med reduceret glycerolproduktion |
US20150307833A1 (en) | 2012-12-12 | 2015-10-29 | Vib Vzw | Identification of thermotolerance genes in yeast |
WO2014170330A2 (en) | 2013-04-15 | 2014-10-23 | Vib Vzw | Yeast alleles involved in maximal alcohol accumulation capacity and tolerance to high alcohol levels |
-
2014
- 2014-04-15 WO PCT/EP2014/057629 patent/WO2014170330A2/en active Application Filing
- 2014-04-15 CA CA2909503A patent/CA2909503A1/en not_active Abandoned
- 2014-04-15 BR BR112015026063A patent/BR112015026063A2/pt not_active Application Discontinuation
- 2014-04-15 AU AU2014255759A patent/AU2014255759B2/en not_active Ceased
- 2014-04-15 EP EP14717780.2A patent/EP2986707A2/de not_active Withdrawn
- 2014-04-15 US US14/784,893 patent/US10000759B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US10000759B2 (en) | 2018-06-19 |
BR112015026063A2 (pt) | 2017-10-17 |
WO2014170330A3 (en) | 2014-12-11 |
EP2986707A2 (de) | 2016-02-24 |
US20160068848A1 (en) | 2016-03-10 |
AU2014255759A1 (en) | 2015-11-05 |
WO2014170330A2 (en) | 2014-10-23 |
CA2909503A1 (en) | 2014-10-23 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Pais et al. | Comparative polygenic analysis of maximal ethanol accumulation capacity and tolerance to high ethanol levels of cell proliferation in yeast | |
Ambroset et al. | Deciphering the molecular basis of wine yeast fermentation traits using a combined genetic and genomic approach | |
Meijnen et al. | Polygenic analysis and targeted improvement of the complex trait of high acetic acid tolerance in the yeast Saccharomyces cerevisiae | |
Gorter de Vries et al. | Industrial relevance of chromosomal copy number variation in Saccharomyces yeasts | |
Wilkening et al. | An evaluation of high-throughput approaches to QTL mapping in Saccharomyces cerevisiae | |
Cubillos et al. | Identification of nitrogen consumption genetic variants in yeast through QTL mapping and bulk segregant RNA-Seq analyses | |
Van den Broek et al. | Chromosomal copy number variation in Saccharomyces pastorianus is evidence for extensive genome dynamics in industrial lager brewing strains | |
García-Ríos et al. | The genetic architecture of low-temperature adaptation in the wine yeast Saccharomyces cerevisiae | |
Swinnen et al. | Genetic mapping of quantitative phenotypic traits in Saccharomyces cerevisiae | |
Ekberg et al. | Adaptive evolution of the lager brewing yeast Saccharomyces pastorianus for improved growth under hyperosmotic conditions and its influence on fermentation performance | |
AU2014255759B2 (en) | Yeast alleles involved in maximal alcohol accumulation capacity and tolerance to high alcohol levels | |
Pérez-Ortı́n et al. | DNA chips for yeast biotechnology. The case of wine yeasts | |
Hauser et al. | Whole genome analysis of a wine yeast strain | |
Maclean et al. | Deciphering the genic basis of yeast fitness variation by simultaneous forward and reverse genetics | |
Krogerus et al. | Enhanced wort fermentation with de novo lager hybrids adapted to high-ethanol environments | |
Swinnen et al. | Auxotrophic mutations reduce tolerance of Saccharomyces cerevisiae to very high levels of ethanol stress | |
Smart | Brewing yeast genomes and genome‐wide expression and proteome profiling during fermentation | |
JP2019088312A (ja) | 酵母株の改良方法 | |
Mangado et al. | Evolution of a yeast with industrial background under winemaking conditions leads to diploidization and chromosomal copy number variation | |
US9862957B2 (en) | Specific alleles important for ethanol tolerance | |
Brouwers et al. | Himalayan Saccharomyces eubayanus genome sequences reveal genetic markers explaining heterotic maltotriose consumption by Saccharomyces pastorianus hybrids | |
Haas et al. | Mapping ethanol tolerance in budding yeast reveals high genetic variation in a wild isolate | |
Ho et al. | Massive QTL analysis identifies pleiotropic genetic determinants for stress resistance, aroma formation, and ethanol, glycerol and isobutanol production in Saccharomyces cerevisiae | |
Fernández-Niño et al. | Identification of novel genes involved in acetic acid tolerance of Saccharomyces cerevisiae using pooled-segregant RNA sequencing | |
US20170191087A1 (en) | Causative genes conferring acetic acid tolerance in yeast |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FGA | Letters patent sealed or granted (standard patent) | ||
MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |