AU2013202930B2 - Microorganisms and methods for producing 1,3-butanediol and related alcohols - Google Patents

Abstract

The invention provides non-naturally occurring microbial organisms containing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathways comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. The invention additionally provides methods of using such microbial organisms to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3 buten-1-ol, by culturing a non- naturally occurring microbial organism containing 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol pathways as described herein under conditions and for a sufficient period of time to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3 buten-1-ol.

Description

1 MICROORGANISMS AND METHOD FOR PRODUCING 1,3-BUTANEDIOL AND RELATED ALCOHOLS The entire disclosure in the complete specification of our Australian Patent Application No. 2012299191 is by this cross-reference incorporated into the present specification BACKGROUND OF THE INVENTION The present invention relates generally to biosynthetic processes, and more specifically to organisms having 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol biosynthetic capability. Over 25 billion pounds of butadiene (1,3-butadiene, BD) are produced annually and is applied in the manufacture of polymers such as synthetic rubbers and ABS resins, and chemicals such as hexamethylenediamine and 1,4-butanediol. Butadiene is typically produced as a by-product of the steam cracking process for conversion of petroleum feedstocks such as naphtha, liquefied petroleum gas, ethane or natural gas to ethylene and other olefins. The ability to manufacture butadiene from alternative and/or renewable feedstocks would represent a major advance in the quest for more sustainable chemical production processes One possible way to produce butadiene renewably involves fermentation of sugars or other feedstocks to produce diols, such as 1,4-butanediol or 1,3-butanediol, which are separated, purified, and then dehydrated to butadiene in a second step involving metal based catalysis. Direct fermentative production of butadiene from renewable feedstocks would obviate the need for dehydration steps and butadiene gas (bp -4.4 0 C) would be continuously emitted from the fermenter and readily condensed and collected. Developing a fermentative production process would eliminate the need for fossil-based butadiene and would allow substantial savings in cost, energy, and harmful waste and emissions relative to petrochemically-derived butadiene.

2 2,4-Pentadienoate is a useful substituted butadiene derivative in its own right and a valuable intermediate en route to other substituted 1,3-butadiene derivatives, including, for example, 1-carbamoyl-1,3-butadienes which are accessible via Curtius rearrangement. The resultant N-protected-1,3-butadiene derivatives can be used in Diels alder reactions 5 for the preparation of substituted anilines. 2,4-Pentadienoate can be used in the preparation of various polymers and co-polymers. 1,3-butanediol (1,3-BDO) is a four carbon diol traditionally produced from acetylene via its hydration. The resulting acetaldehyde is then converted to 3-hydroxybutyraldehdye which is subsequently reduced to form 1,3-BDO. In more recent years, acetylene has been 10 replaced by the less expensive ethylene as a source of acetaldehyde. 1,3-BDO is commonly used as an organic solvent for food flavoring agents. It is also used as a co monomer for polyurethane and polyester resins and is widely employed as a hypoglycaemic agent. Optically active 1,3-BDO is a useful starting material for the synthesis of biologically active compounds and liquid crystals. A commercial use of 1,3 15 butanediol is subsequent dehydration to afford 1,3-butadiene (Ichikawa et al., J. of Molecular Catalysis A-Chemical, 256:106-112 (2006); Ichikawa et al., J. of Molecular Catalysis A-Chemical, 231:181-189 (2005)), a 25 billion lb/yr petrochemical used to manufacture synthetic rubbers (e.g., tires), latex, and resins. The reliance on petroleum based feedstocks for either acetylene or ethylene warrants the development of a renewable 20 feedstock based route to 1,3-butanediol and to butadiene. 3-Buten- 1 -ol is a raw material used in pharmaceuticals, agrochemicals, perfumes and resins. The palladium-catalyzed coupling of 3-buten- 1 -ol with aryl halides is a valuable process for the preparation of aryl-substituted aldehydes such as, for example, the antifolate compound Pemetrexed disodium (R. C. Larock et al., Tetrahedron Letters, 30, 25 6629 (1989) andU.S. Pat. No. 6,262,262). 3-Buten-1-ol is commonly prepared from propylene and formaldehyde in the presence of a catalyst at high temperature and pressure. Alternately, it is prepared from 3,4-epoxy-1-butene. Preparation of 3-buten-1-ol from renewable feedstocks has not been demonstrated to date. Propylene is produced primarily as a by-product of petroleum refining and of ethylene 30 production by steam cracking of hydrocarbon feedstocks. Propene is separated by fractional distillation from hydrocarbon mixtures obtained from cracking and other refining processes. Typical hydrocarbon feedstocks are from non-renewable fossil fuels, such as petroleum, natural gas and to a much lesser extent coal. Over 75 billion pounds of 3 propylene are manufactured annually, making it the second largest fossil-based chemical produced behind ethylene. Propylene is a base chemical that is converted into a wide range of polymers, polymer intermediates and chemicals. Some of the most common derivatives of chemical and polymer grade propylene are polypropylene, acrylic acid, 5 butanol, butanediol, acrylonitrile, propylene oxide, isopropanol and cumene. The use of the propylene derivative, polypropylene, in the production of plastics, such as injection moulding, and fibers, such as carpets, accounts for over one-third of U.S. consumption for this derivative. Propylene is also used in the production of synthetic rubber and as a propellant or component in aerosols. 10 The ability to manufacture propylene from alternative and/or renewable feedstocks would represent a major advance in the quest for more sustainable chemical production processes. One possible way to produce propylene renewably involves fermentation of sugars or other feedstocks to produce the alcohols 2-propanol (isopropanol) or 1 -propanol, which is separated, purified, and then dehydrated to propylene in a second step involving 15 metal-based catalysis. Direct fermentative production of propylene from renewable feedstocks would obviate the need for dehydration. During fermentative production, propylene gas would be continuously emitted from the fermenter, which could be readily collected and condensed. Developing a fermentative production process would also eliminate the need for fossil-based propylene and would allow substantial savings in cost, 20 energy, and harmful waste and emissions relative to petrochemically-derived propylene. Crotyl alcohol, also referred to as 2-buten- 1 -ol, is a valuable chemical intermediate. It serves as a precursor to crotyl halides, esters, and ethers, which in turn are chemical intermediates in the production of monomers, fine chemicals, agricultural chemicals, and pharmaceuticals. Exemplary fine chemical products include sorbic acid, 25 trimethylhydroquinone, crotonic acid and 3-methoxybutanol. Crotyl alcohol is also a precursor to 1,3-butadiene. Crotyl alcohol is currently produced exclusively from petroleum feedstocks. For example Japanese Patent 47-013009 and U.S. Pat. Nos. 3,090,815, 3,090,816, and 3,542,883 describe a method of producing crotyl alcohol by isomerization of 1,2-epoxybutane. The ability to manufacture crotyl alcohol from 30 alternative and/or renewable feedstocks would represent a major advance in the quest for more sustainable chemical production processes. Thus, there exists a need for alternative methods for effectively producing commercial quantities of compounds such as 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, 4 (followed by 4A) crotyl alcohol or 3-buten- I -ol. The present invention satisfies this need and provides related advantages as well. SUMMARY OF INVENTION The invention provides a non-naturally occurring microbial organism, a 1,3-butanediol pathway comprising exogenous nucleic acids encoding 1,3-butanediol pathway enzymes expressed in a sufficient amount to produce 1,3-butanediol, wherein said 1,3-butanediol pathway comprises a pathway selected from: (6) 7A, 7D, 7E, 7F, 7G and 7S; (7) 7A, 7D, 71, 7G and 7S; (8) 7A, 7D, 7K, and 7S; (9) 7A, 7H, 7F, 7G and 7S; (10) 7A, 7J, 7G and 7S; (11) 7A, 7J, 7R and 7AA; (12) 7A, 7H, 7F, 7R and 7AA; (13) 7A, 7H, 7Q, 7Z and 7AA; (14) 7A, 7D, 71, 7R and 7AA; (15) 7A, 7D, 7E, 7F, 7R and 7AA; (16) 7A, 7D, 7E, 7Q, 7Z and 7AA; (17) 7A, 7D, 7P, 7N and 7AA; (18) 7A, 7D, 7P, 7Y, 7Z and 7AA; (19) 7A, 7B, 7M and 7AA; (20) 7A, 7B, 7L, 7Z and 7AA; (21) 7A, 7B, 7X, 7N and 7AA; (22) 7A, 7B, 7X, 7Y, 7Z and 7AA; (23) 7A, 7D, 7P and 70; 4A (followed by 4B) (24) 7A, 7B, 7X and 70; (25) 7A, 7D, 7E, 7F, 7R, 7AA; (26) 7A, 7D, 7E, 7F, 7G, 7S; wherein 7A is a 3-ketoacyl-ACP synthase catalyzing conversion of malonyl-ACP to acetoacetyl-ACP, wherein 7B is an acetoacetyl-ACP reductase catalyzing conversion of acetoacetyl-ACP to 3-hydroxybutyryl-ACP, wherein 7D is an acetoacetyl-CoA:ACP transferase catalyzing conversion of acetoacetyl-ACP to acetoacetyl-CoA, wherein 7E is an acetoacetyl-CoA hydrolase, acetoacetyl-CoA transferase or acetoacetyl-CoA synthetase catalyzing conversion of acetoacetyl-CoA to acetoacetate, wherein 7F is an acetoacetate reductase (acid reducing) catalyzing conversion of acetoacetate to 3-oxobuytraldehyde, wherein 7G is a 3-oxobutyraldehyde reductase (aldehyde reducing) catalyzing conversion of 3-oxobutyraldehyde to 4-hydroxy-2-butanone, wherein 7H is an acetoacetyl-ACP thioesterase catalyzing conversion ofacetoacetyl-ACP to acetoacetate, wherein 71 is an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming) catalyzing conversion of acetoacetyl-CoA to 3-oxobuytraldehyde, wherein 7J is an acetoacetyl-ACP reductase (aldehyde forming) catalyzing conversion of acetoacetyl-ACP to 3-oxobutyraldehyde, wherein 7K is an acetoacetyl-CoA reductase (alcohol forming) catalyzing conversion of acetoacetyl-CoA to 4-hydroxy-2-butanone, wherein 7L is a 3-hydroxybutyryl-ACP thioesterase catalyzing conversion of 3-hydroxybutyryl-ACP to 3-hydroxybutyrate, wherein 7M is a 3-hydroxybutyryl-ACP reductase (aldehyde forming) catalyzing conversion of 3 hydroxybutyryl-ACP to 3-hydroxybutyraldehdye, wherein 7N is a 3-hydroxybutyryl-CoA reductase (aldehyde forming) catalyzing conversion of 3-hydroxybutyryl-CoA to 3 hydroxybutyraldehyde, wherein 70 is a 3-hydroxybutyryl-CoA reductase (alcohol forming) catalyzing conversion of 3-hydroxybutyryl-CoA to 1,3-butanediol, wherein 7P is an acetoacetyl-CoA reductase (ketone reducing) catalyzing conversion of acetoacetyl-CoA to 3 hydroxybutyryl-CoA, wherein 7Q is an acetoacetate reductase (ketone reducing) catalyzing conversion of acetoacetate to 3-hydroxybutyrate, wherein 7R is a 3-oxobutyraldehyde reductase (ketone reducing) catalyzing conversion of 3-oxobutyraldehyde to 3 hydroxybutyraldehyde, wherein 7S is a 4-hydroxy-2-butanone reductase catalyzing conversion of4-hydroxy-2-butanone to 1,3-butanediol, wherein 7X is a 3-hydroxybutyryl CoA:ACP transferase catalyzing conversion of 3-hydroxybutyryl-ACP to 3-hydroxybutyryl CoA, wherein 7Y is a 3-hydroxybutyryl-CoA hydrolase, a 3-hydroxybutyryl-CoA transferase 4B (followed by 4C) or a 3-hydroxybutyryl-CoA synthetase catalyzing conversion of 3-hydroxybutyryl CoA to 3-hydroxybutyrate, wherein 7Z is a 3-hydroxybutyrate reductase catalyzing conversion of 3-hydroxybutyrate to 3-hydroxybutyraldehdye, and wherein 7AA is a 3 hydroxybutyraldehyde reductase catalyzing conversion of 3-hydroxybutyraldehyde to 1,3 butanediol. Also provided by the present invention is a method of producing 1,3-butanediol comprising culturing such a microbial organism under conditions and for a sufficient period of time to produce 1,3- butanediol. Although not the subject of the present application, also described herein for completeness are non-naturally occurring microbial organisms containing 2,4-pentadienoate, butadiene, propylene, crotyl alcohol or 3-buten-1-ol pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce 2,4 pentadienoate, butadiene, propylene, crotyl alcohol or 3-buten-1-ol. Also described are methods of using such microbial organisms to produce 2,4-pentadienoate, butadiene, propylene, crotyl alcohol or 3-buten-1-ol, by culturing a non-naturally occurring microbial organism containing 2,4-pentadienoate, butadiene, propylene, crotyl alcohol or 3-buten-1-ol pathways as described herein under conditions and for a sufficient period of time to produce 2,4-pentadienoate, butadiene, propylene, crotyl alcohol or 3-buten-1-ol. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 shows exemplary pathways to 3-bute-i1-ol, 2,4-pentadienoate and butadiene from 2-oxoadipate, 2-aminoadipate, 5-aminopentanoate and glutarykCoA. Enzymes are: A. 2-arninoadipate decarboxylase, B. 5-aninopentanoate reductase, C. 5-aminopent-2 enoate aminotransferase, dehydrogenase or amine oxidasc, D. 2 -oxoadipate decarboxylase, E. glutarate semialdehyde reductase, F. 5-hydroxyvalerate dchydrogenase. G. 5-hydroxypent-2-enoate dehydratase, H. 2-am inoadipate aminotransferase, dehydrogenase or amine oxidase, 1. 5-arninopentanoate aminotransferase, dehydrogenase or amine oxidase, 1. 5-aminopent-2-enoate deaminase, K. 5-hydroxypent-2-enoate reductase, L. 5-bydroxyvaleryl-CoA transferase and/or synthetase, A. 5 hydroxypentanoyl-CoA dehydrogenase, N. 5-hydroxypent-2-enoyb-CoA dehydratase, O. 2,4-pentadicnoy 1 -CoA transferase, synthetase or hydrolase, P. 5-bydroxypent-2-enoyl CoA transferase or synthetase, Q. 5-hydroxyvalery -CoA dcliydratase/dehydrogen.ase, R.

4C (followed by 5) 2-oxoadipate dehydrogenase, 2-oxoadipate;ferridoxin oxidoreductase or 2-oxoadipate formate lyase., S. glutaryl-CoA reductase, T. 2,4-pentadienoate decarboxylase, U. 5 hydroxypent-2-enoate decarboxylase, V, 3-buten- 1 -of dehydratase or chemical conversion, W, 5-hydroxyvalerate decarboxylase, 5 Figure 2 shows an exemplary carbon-efficient pathway from acetyl-CoA to the 2,4 pentadienoate precursor glutaryl-CoA. Enzymes are: A. acetoacetyl-CoA thiolase or synthase, B. acetoacetyl-CoA reductase, C. 3-hydroxybutyryl-CoA dehydratase, D. glutaryl-CoA dehydrogenase. 5 Figure 3 shows exemplary pathways for conversion of propionyl-CoA to 2,4 pentadienoate. Enzymes are: A. 3-oxopentanoyl-CoA thiolase or synthase, B. 3 oxopentanoyl-CoA reductase, C. 3-hydroxypentanoyl-CoA dehydratase, D. pent-2-enoyl CoA isomerase, E. pent-3-enoyl-CoA dehydrogenase, F. 2,4-pentadienoyl-CoA hydrolase, transferase or synthetase, G. pent-2-enoyl-CoA dehydrogenase. 10 Figure 4 shows an exemplary pathway for 1,3-butanediol formation from 3 hydroxypropionyl-CoA and acetyl-CoA. Enzymes are: A. 3-oxo-5-hydroxypentanoyl CoA thiolase or synthase, B. 3-oxo-5-hydroxypentanoyl-CoA hydrolase, transferase or synthetase, C. 3-oxo-5-hydroxypentanoate decarboxylase and D. 3-oxobutanol reductase. Figure 5 shows exemplary pathways to 1,3-butanediol (13-BDO), 3-buten-1-ol and 15 butadiene from pyruvate and acetaldehyde. Enzymes are: A. 4-hydroxy-2-oxovalerate aldolase, B. 4-hydroxy-2-oxovalerate dehydratase, C. 2-oxopentenoate decarboxylase, D. 3-buten-1-al reductase, E. 3-buten-1-ol dehydratase, F. 4-hydroxy-2-oxovalerate decarboxylase, G. 3-hydroxybutanal reductase, H. 4-hydroxy-2-oxopentanoate dehydrogenase, 4-hydroxy-2-oxopentanoate:ferredoxin oxidoreductase or 4-hydroxy-2 20 oxopentanoate formate lyase, . 3-hydroxybutyryl-CoA reductase (aldehyde forming), 1. 3 hydroxybutyryl-CoA hydrolase, transferase or synthetase, K. 3-hydroxybutyrate reductase, L. 3-hydroxybutyryl-CoA reductase (alcohol forming). Step E can also be catalyzed via chemical dehydration. Figure 6 shows exemplary pathways to butadiene from 2,4-pentadienoate and 2,4 25 pentadienoyl-CoA. Enzymes are: A. 2,4-pentadienoate reductase (acid reducing), B. penta-2,4-dienal decarbonylase, C. 2,4-pentadienoyl-CoA reductase (acid reducing), D. 2,4-pentadienoyl-CoA phosphotransferase, E. 2,4-pentadienoyl-phosphate reductase, F. 2,4-pentadienoyl-CoA hydrolase, transferase or synthetase, G. 2,4-pentadienoate decarboxylase, H. 2,4-pentadienoate kinase. 30 Figure 7 shows exemplary pathways for formation of 1,3-butanediol, crotyl alcohol and propylene from malonyl-ACP. Enyzmes are: A. 3-ketoacyl-ACP synthase, B. Acetoacetyl ACP reductase, C. 3-hydroxybutyryl-ACP dehydratase, D. acetoacetyl-CoA:ACP 6 transferase, E. acetoacetyl-CoA hydrolase, transferase or synthetase, F. acetoacetate reductase (acid reducing), G. 3-oxobutyraldehyde reductase (aldehyde reducing), H. acetoacetyl-ACP thioesterase, I. acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), J. acetoacetyl-ACP reductase (aldehyde forming), K. acetoacetyl-CoA reductase 5 (alcohol forming), L. 3-hydroxybutyryl-ACP thioesterase, M. 3-hydroxybutyryl-ACP reductase (aldehyde forming), N. 3-hydroxybutyryl-CoA reductase (aldehyde forming), 0. 3-hydroxybutyryl-CoA reductase (alcohol forming), P. acetoacetyl-CoA reductase (ketone reducing), Q. acetoacetate reductase (ketone reducing), R. 3-oxobutyraldehyde reductase (ketone reducing), S. 4-hydroxy-2-butanone reductase, T. crotonyl-ACP thioesterase, U. 10 crotonyl-ACP reductase (aldehyde forming), V. crotonyl-CoA reductase (aldehyde forming), W. crotonyl-CoA (alcohol forming), X. 3-hydroxybutyryl-CoA:ACP transferase, Y. 3-hydroxybutyryl-CoA hydrolase, transferase or synthetase, Z. 3 hydroxybutyrate reductase, AA. 3-hydroxybutyraldehyde reductase, AB. 3 hydroxybutyryl-CoA dehydratase, AC. 3-hydroxybutyrate dehydratase, AD. 3 15 hydroxybutyraldehyde dehydratase, AE. crotonyl-CoA:ACP transferase, AF. crotonyl CoA hydrolase, transferase or synthetase, AG. crotonate reductase, AH. crotonaldehyde reductase, Al. Butryl-CoA:ACP transferase, AJ. Butyryl-CoA transferase, hydrolase or synthetase, AK. Butyrate decarboxylase, AL. crotonyl-ACP reductase, AM. crotonyl-CoA reductase, AN. crotonate reductase, AO. crotonaldehyde decarbonylase, AP. butyryl-ACP 20 thioesterase, AQ. crotonate decarboxylase, AR. 3-hydroxybutyrate decarboxylase, AS. acetoacetyl-CoA synthase. ACP is acyl carrier protein. Figure 8 shows the reverse TCA cycle for fixation of CO 2 on carbohydrates as substrates. The enzymatic transformations are carried out by the enzymes as shown. Figure 9 shows the pathway for the reverse TCA cycle coupled with carbon monoxide 25 dehydrogenase and hydrogenase for the conversion of syngas to acetyl-CoA. Figure 10 shows Western blots of 10 micrograms ACS90 (lane 1), ACS91 (lane2), Mta98/99 (lanes 3 and 4) cell extracts with size standards (lane 5) and controls of M. thermoacetica CODH (Moth 1202/1203) or Mtr (Moth_1197) proteins (50, 150, 250, 350, 450, 500, 750, 900, and 1000 ng). 30 Figure 11 shows CO oxidation assay results. Cells (M. thermoacetica or E. coli with the CODH/ACS operon; ACS90 or ACS91 or empty vector: pZA33S) were grown and extracts prepared. Assays were performed at 55*C at various times on the day the extracts 7 were prepared. Reduction of methylviologen was followed at 578 nm over a 120 sec time course. Figure 12 shows pathways for conversion of crotyl alcohol to butadiene. Enzymes are: A. crotyl alcohol kinase, B. 2-butenyl-4-phosphate kinase, C. butadiene synthase, and D. 5 crotyl alcohol diphosphokinase. Step E is catalyzed non-enzymatically. DETAILED DESCRIPTION OF THE INVENTION The present invention is directed to the design and production of cells and organisms having biosynthetic production capabilities for 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. The invention, in particular, relates to the 10 design of microbial organisms capable of producing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol by introducing one or more nucleic acids encoding a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway enzyme. In one embodiment, the invention utilizes in silico stoichiometric models of Escherichia 15 coli metabolism that identify metabolic designs for biosynthetic production of 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol. The results described herein indicate that metabolic pathways can be designed and recombinantly engineered to achieve the biosynthesis of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol in Escherichia coli and other 20 cells or organisms. Biosynthetic production of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- I -ol, for example, for the in silico designs can be confirmed by construction of strains having the designed metabolic genotype. These metabolically engineered cells or organisms also can be subjected to adaptive evolution to further augment butadiene biosynthesis, including under conditions approaching 25 theoretical maximum growth. In certain embodiments, the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol biosynthesis characteristics of the designed strains make them genetically stable and particularly useful in continuous bioprocesses. Separate strain design strategies were identified with incorporation of different non-native or heterologous 30 reaction capabilities into E. coli or other host organisms leading to 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol producing metabolic pathways from 2-aminoadipate, 5-aminopentanoate, 2-oxoadipate, glutaryl-CoA, acetyl- 8 CoA, propionyl-CoA, 3-hydroxypropionyl-CoA or pyruvate. In silico metabolic designs were identified that resulted in the biosynthesis of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol in microorganisms from each of these substrates or metabolic intermediates. 5 Strains identified via the computational component of the platform can be put into actual production by genetically engineering any of the predicted metabolic alterations, which lead to the biosynthetic production of 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten-1-ol or other intermediate and/or downstream products. In yet a further embodiment, strains exhibiting biosynthetic production of these 10 compounds can be further subjected to adaptive evolution to further augment product biosynthesis. The levels of product biosynthesis yield following adaptive evolution also can be predicted by the computational component of the system. The maximum theoretical 2,4-pentadienoate yield from glucose is 1.09 mol/mol (0.59 g/g). 15 11 C 6

H

12 0 6 = 12 C 5

H

6 0 2 + 6 C0 2 + 30 H 2 0 The pathways presented in Figure 1 achieve a yield of 0.85 moles 2,4-pentadienoate per mole of glucose utilized. Increasing product yields is possible if cells are capable of fixing

CO

2 through pathways such as the reductive (or reverse) TCA cycle or the Wood Ljungdahl pathway. Organisms engineered to possess the pathway depicted in Figure 1 20 are also capable of reaching near theoretical maximum yields of 2,4-pentadienoate. The maximum theoretical butadiene yield from glucose is 1.09 mol/mol (0.327g/g). 11 C 6

H

1 2 0 6 = 12 C 4

H

6 + 18 C0 2 + 30 H 2 0 The pathways presented in Figure 1 achieves a yield of 0.85 moles butadiene per mole of glucose utilized. Increasing product yields to near theoretical maximum values is possible 25 if cells are capable of fixing CO 2 through pathways such as the reductive (or reverse) TCA cycle or the Wood-Ljungdahl pathway. Organisms engineered to possess a pathway depicted in Figures 5, 6 or Figure 1 in combination with a pathways depicted in Figure 12 are also capable of reaching near theoretical maximum yields of butadiene. The maximum theoretical 1,3-butanediol yield from glucose is 1.09 mol/mol (0.54 g/g).

9 11 C 6

H

12 0 6 = 12 C 4

H

10 0 2 + 18 CO 2 + 6 H 2 0 The pathways presented in Figure 5 achieve a yield of 1 moles 1,3-butanediol per mole of glucose utilized. Increasing product yields to theoretical maximum value is possible if cells are capable of fixing CO 2 through pathways such as the reductive (or reverse) TCA 5 cycle or the Wood-Ljungdahl pathway. Organisms engineered to possess the pathways depicted in Figure 7 are also capable of reaching theoretical maximum yields of 1,3 butanediol. The maximum theoretical 3-buten-1-ol yield from glucose is 1.09 mol/mol (0.437 g/g). 11 C 6

H

12 0 6 = 12 C 4 HsO + 18 CO 2 + 18 H20 10 The pathways presented in Figure 1 achieve a yield of 0.85 moles 3-buten- 1 -ol per mole of glucose utilized. Increasing product yields to nearly the theoretical maximum is possible if cells are capable of fixing CO 2 through pathways such as the reductive (or reverse) TCA cycle or the Wood-Ljungdahl pathway. Organisms engineered to possess the pathway depicted in Figure 5 are also capable of reaching near theoretical maximum yields of 15 butadiene. The maximum theoretical crotyl alcohol yield from glucose is 1.09 mol/mol (0.436 g/g). 11 C 6

H

12 0 6 = 12 C 4

H

8 0 + 18 CO 2 + 18 H20 The pathways presented in Figure 7 achieve a yield of 1.08 moles crotyl alcohol per mole of glucose utilized. Increasing product yields to the theoretical maximum is possible if 20 cells are capable of fixing CO 2 through pathways such as the reductive (or reverse) TCA cycle or the Wood-Ljungdahl pathway. The maximum theoretical propylene yield from glucose is 1.33 mol/mol (0.31 g/g). 3 C 6

H

12

O

6 = 4 C 4 H0 + 6 C02 + 6 H 2 0 The pathways presented in Figure 7 achieve a yield of 1.2 moles propylene per mole of 25 glucose utilized. Increasing product yields to nearly the theoretical maximum is possible if cells are capable of fixing CO 2 through pathways such as the reductive (or reverse) TCA cycle or the Wood-Ljungdahl pathway.

10 As used herein, the term "non-naturally occurring" when used in reference to a microbial organism or microorganism of the invention is intended to mean that the microbial organism has at least one genetic alteration not normally found in a naturally occurring strain of the referenced species, including wild-type strains of the referenced species. 5 Genetic alterations include, for example, modifications introducing expressible nucleic acids encoding metabolic polypeptides, other nucleic acid additions, nucleic acid deletions and/or other functional disruption of the microbial organism's genetic material. Such modifications include, for example, coding regions and functional fragments thereof, for heterologous, homologous or both heterologous and homologous polypeptides for the 10 referenced species. Additional modifications include, for example, non-coding regulatory regions in which the modifications alter expression of a gene or operon. Exemplary metabolic polypeptides include enzymes or proteins within a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol biosynthetic pathway. A metabolic modification refers to a biochemical reaction that is altered from its naturally 15 occurring state. Therefore, non-naturally occurring microorganisms can have genetic modifications to nucleic acids encoding metabolic polypeptides, or functional fragments thereof. Exemplary metabolic modifications are disclosed herein, As used herein, the term "isolated" when used in reference to a microbial organism is intended to mean an organism that is substantially free of at least one component as the 20 referenced microbial organism is found in nature. The term includes a microbial organism that is removed from some or all components as it is found in its natural environment. The term also includes a microbial organism that is removed from some or all components as the microbial organism is found in non-naturally occurring environments. Therefore, an isolated microbial organism is partly or completely separated from other substances as it is 25 found in nature or as it is grown, stored or subsisted in non-naturally occurring environments. Specific examples of isolated microbial organisms include partially pure microbes, substantially pure microbes and microbes cultured in a medium that is non naturally occurring. As used herein, the terms "microbial," "microbial organism" or "microorganism" are 30 intended to mean any organism that exists as a microscopic cell that is included within the domains of archaea, bacteria or eukarya. Therefore, the term is intended to encompass prokaryotic or eukaryotic cells or organisms having a microscopic size and includes bacteria, archaea and eubacteria of all species as well as eukaryotic microorganisms such 11 as yeast and fungi. The term also includes cell cultures of any species that can be cultured for the production of a biochemical. As used herein, the term "CoA" or "coenzyme A" is intended to mean an organic cofactor or prosthetic group (nonprotein portion of an enzyme) whose presence is required for the 5 activity of many enzymes (the apoenzyme) to form an active enzyme system. Coenzyme A functions in certain condensing enzymes, acts in acetyl or other acyl group transfer and in fatty acid synthesis and oxidation, pyruvate oxidation and in other acetylation. As used herein, the term "ACP" or "acyl carrier protein" refers to any of the relatively small acidic proteins that are associated with the fatty acid synthase system of many 10 organisms, from bacteria to plants. ACPs can contain one 4'-phosphopantetheine prosthetic group bound covalently by a phosphate ester bond to the hydroxyl group of a serine residue. The sulfhydryl group of the 4'-phosphopantetheine moiety serves as an anchor to which acyl intermediates are (thio)esterified during fatty-acid synthesis. An example of an ACP is Escherichia coli ACP, a separate single protein, containing 77 15 amino-acid residues (8.85 kDa), wherein the phosphopantetheine group is linked to seine 36. As used herein, the term "butadiene," having the molecular formula C 4

H

6 and a molecular mass of 54.09 g/mol (see Figures 1, 5, 6 and 12) (IUPAC name Buta-1,3-diene) is used interchangeably throughout with 1,3-butadiene, biethylene, erythrene, divinyl, 20 vinylethylene. Butadiene is a colorless, non corrosive liquefied gas with a mild aromatic or gasoline-like odor. Butadiene is both explosive and flammable because of its low flash point. As used herein, the term "substantially anaerobic" when used in reference to a culture or growth condition is intended to mean that the amount of oxygen is less than about 10% of 25 saturation for dissolved oxygen in liquid media. The term also is intended to include sealed chambers of liquid or solid medium maintained with an atmosphere of less than about 1% oxygen. "Exogenous" as it is used herein is intended to mean that the referenced molecule or the referenced activity is introduced into the host microbial organism. The molecule can be 30 introduced, for example, by introduction of an encoding nucleic acid into the host genetic material such as by integration into a host chromosome or as non-chromosomal genetic material such as a plasmid. Therefore, the term as it is used in reference to expression of 12 an encoding nucleic acid refers to introduction of the encoding nucleic acid in an expressible form into the microbial organism. When used in reference to a biosynthetic activity, the term refers to an activity that is introduced into the host reference organism. The source can be, for example, a homologous or heterologous encoding nucleic acid that 5 expresses the referenced activity following introduction into the host microbial organism. Therefore, the term "endogenous" refers to a referenced molecule or activity that is present in the host. Similarly, the term when used in reference to expression of an encoding nucleic acid refers to expression of an encoding nucleic acid contained within the microbial organism. The term "heterologous" refers to a molecule or activity derived 10 from a source other than the referenced species whereas "homologous" refers to a molecule or activity derived from the host microbial organism. Accordingly, exogenous expression of an encoding nucleic acid of the invention can utilize either or both a heterologous or homologous encoding nucleic acid. It is understood that when more than one exogenous nucleic acid is included in a microbial 15 organism that the more than one exogenous nucleic acids refers to the referenced encoding nucleic acid or biosynthetic activity, as discussed above. It is further understood, as disclosed herein, that such more than one exogenous nucleic acids can be introduced into the host microbial organism on separate nucleic acid molecules, on polycistronic nucleic acid molecules, or a combination thereof, and still be considered as more than one 20 exogenous nucleic acid. For example, as disclosed herein a microbial organism can be engineered to express two or more exogenous nucleic acids encoding a desired pathway enzyme or protein. In the case where two exogenous nucleic acids encoding a desired activity are introduced into a host microbial organism, it is understood that the two exogenous nucleic acids can be introduced as a single nucleic acid, for example, on a 25 single plasmid, on separate plasmids, can be integrated into the host chromosome at a single site or multiple sites, and still be considered as two exogenous nucleic acids. Similarly, it is understood that more than two exogenous nucleic acids can be introduced into a host organism in any desired combination, for example, on a single plasmid, on separate plasmids, can be integrated into the host chromosome at a single site or multiple 30 sites, and still be considered as two or more exogenous nucleic acids, for example three exogenous nucleic acids. Thus, the number of referenced exogenous nucleic acids or biosynthetic activities refers to the number of encoding nucleic acids or the number of biosynthetic activities, not the number of separate nucleic acids introduced into the host organism.

13 The non-naturally occurring microbal organisms of the invention can contain stable genetic alterations, which refers to microorganisms that can be cultured for greater than five generations without loss of the alteration. Generally, stable genetic alterations include modifications that persist greater than 10 generations, particularly stable modifications 5 will persist more than about 25 generations, and more particularly, stable genetic modifications will be greater than 50 generations, including indefinitely. Those skilled in the art will understand that the genetic alterations, including metabolic modifications exemplified herein, are described with reference to a suitable host organism such as E. coli and their corresponding metabolic reactions or a suitable source organism 10 for desired genetic material such as genes for a desired metabolic pathway. However, given the complete genome sequencing of a wide variety of organisms and the high level of skill in the area of genomics, those skilled in the art will readily be able to apply the teachings and guidance provided herein to essentially all other organisms. For example, the E. coli metabolic alterations exemplified herein can readily be applied to other species 15 by incorporating the same or analogous encoding nucleic acid from species other than the referenced species. Such genetic alterations include, for example, genetic alterations of species homologs, in general, and in particular, orthologs, paralogs or nonorthologous gene displacements. An ortholog is a gene or genes that are related by vertical descent and are responsible for 20 substantially the same or identical functions in different organisms. For example, mouse epoxide hydrolase and human epoxide hydrolase can be considered orthologs for the biological function of hydrolysis of epoxides. Genes are related by vertical descent when, for example, they share sequence similarity of sufficient amount to indicate they are homologous, or related by evolution from a common ancestor. Genes can also be 25 considered orthologs if they share three-dimensional structure but not necessarily sequence similarity, of a sufficient amount to indicate that they have evolved from a common ancestor to the extent that the primary sequence similarity is not identifiable. Genes that are orthologous can encode proteins with sequence similarity of about 25% to 100% amino acid sequence identity. Genes encoding proteins sharing an amino acid 30 similarity less that 25% can also be considered to have arisen by vertical descent if their three-dimensional structure also shows similarities. Members of the seine protease family of enzymes, including tissue plasminogen activator and elastase, are considered to have arisen by vertical descent from a common ancestor.

14 Orthologs include genes or their encoded gene products that through, for example, evolution, have diverged in structure or overall activity. For example, where one species encodes a gene product exhibiting two functions and where such functions have been separated into distinct genes in a second species, the three genes and their corresponding 5 products are considered to be orthologs. For the production of a biochemical product, those skilled in the art will understand that the orthologous gene harboring the metabolic activity to be introduced or disrupted is to be chosen for construction of the non-naturally occurring microorganism. An example of orthologs exhibiting separable activities is where distinct activities have been separated into distinct gene products between two or 10 more species or within a single species. A specific example is the separation of elastase proteolysis and plasminogen proteolysis, two types of serine protease activity, into distinct molecules as plasminogen activator and elastase. A second example is the separation of mycoplasma 5'-3' exonuclease and Drosophila DNA polymerase III activity. The DNA polymerase from the first species can be considered an ortholog to either or both of the 15 exonuclease or the polymerase from the second species and vice versa. In contrast, paralogs are homologs related by, for example, duplication followed by evolutionary divergence and have similar or common, but not identical functions. Paralogs can originate or derive from, for example, the same species or from a different species. For example, microsomal epoxide hydrolase (epoxide hydrolase I) and soluble 20 epoxide hydrolase (epoxide hydrolase II) can be considered paralogs because they represent two distinct enzymes, co-evolved from a common ancestor, that catalyze distinct reactions and have distinct functions in the same species. Paralogs are proteins from the same species with significant sequence similarity to each other suggesting that they are homologous, or related through co-evolution from a common ancestor. Groups of 25 paralogous protein families include HipA homologs, luciferase genes, peptidases, and others. A nonorthologous gene displacement is a nonorthologous gene from one species that can substitute for a referenced gene function in a different species. Substitution includes, for example, being able to perform substantially the same or a similar function in the species 30 of origin compared to the referenced function in the different species. Although generally, a nonorthologous gene displacement will be identifiable as structurally related to a known gene encoding the referenced function, less structurally related but functionally similar genes and their corresponding gene products nevertheless will still fall within the meaning 15 of the term as it is used herein. Functional similarity requires, for example, at least some structural similarity in the active site or binding region of a nonorthologous gene product compared to a gene encoding the function sought to be substituted. Therefore, a nonorthologous gene includes, for example, a paralog or an unrelated gene. 5 Therefore, in identifying and constructing the non-naturally occurring microbial organisms of the invention having 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol biosynthetic capability, those skilled in the art will understand with applying the teaching and guidance provided herein to a particular species that the identification of metabolic modifications can include identification and inclusion or 10 inactivation of orthologs. To the extent that paralogs and/or nonorthologous gene displacements are present in the referenced microorganism that encode an enzyme catalyzing a similar or substantially similar metabolic reaction, those skilled in the art also can utilize these evolutionally related genes. Orthologs, paralogs and nonorthologous gene displacements can be determined by 15 methods well known to those skilled in the art. For example, inspection of nucleic acid or amino acid sequences for two polypeptides will reveal sequence identity and similarities between the compared sequences. Based on such similarities, one skilled in the art can determine if the similarity is sufficiently high to indicate the proteins are related through evolution from a common ancestor. Algorithms well known to those skilled in the art, 20 such as Align, BLAST, Clustal W and others compare and determine a raw sequence similarity or identity, and also determine the presence or significance of gaps in the sequence which can be assigned a weight or score. Such algorithms also are known in the art and are similarly applicable for determining nucleotide sequence similarity or identity. Parameters for sufficient similarity to determine relatedness are computed based on well 25 known methods for calculating statistical similarity, or the chance of finding a similar match in a.random polypeptide, and the significance of the match determined. A computer comparison of two or more sequences can, if desired, also be optimized visually by those skilled in the art. Related gene products or proteins can be expected to have a high similarity, for example, 25% to 100% sequence identity. Proteins that are unrelated 30 can have an identity which is essentially the same as would be expected to occur by chance, if a database of sufficient size is scanned (about 5%). Sequences between 5% and 24% may or may not represent sufficient homology to conclude that the compared sequences are related. Additional statistical analysis to determine the significance of such 16 matches given the size of the data set can be carried out to determine the relevance of these sequences. Exemplary parameters for determining relatedness of two or more sequences using the BLAST algorithm, for example, can be as set forth below. Briefly, amino acid sequence 5 alignments can be performed using BLASTP version 2.0.8 (Jan-05-1999) and the following parameters: Matrix: 0 BLOSUM62; gap open: 11; gap extension: 1; xdropoff: 50; expect: 10.0; wordsize: 3; filter: on. Nucleic acid sequence alignments can be performed using BLASTN version 2.0.6 (Sept-16-1998) and the following parameters: Match: 1; mismatch: -2; gap open: 5; gap extension: 2; xdropoff: 50; expect: 10.0; 10 wordsize: 11; filter: off. Those skilled in the art will know what modifications can be made to the above parameters to either increase or decrease the stringency of the comparison, for example, and determine the relatedness of two or more sequences. In some embodiments, the invention provides a non-naturally occurring microbial organism, having a microbial organism having a 2,4-pentadienoate pathway having at least 15 one exogenous nucleic acid encoding a 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce 2,4-pentadienoate, wherein the 2,4-pentadienoate pathway includes a pathway shown in Figures 1 and/or 3 selected from: (1) 1D, 11, 1B, IC, 1K and IG; (2) ID, 1E, 1F and IG; (3) 1D, 1E, IL, IM, 1P and 1G; (4) ID, 11, lB and 1J; (5) 1D, 11, 1B, IC, 1K, 1P, 1N and 10; (6) ID, lE, 1F, IP, 1N and 10; (7) ID, 1E, IL, 1M, 1N 20 and 10; (8) ID, IE, IL, IQ and 10; (9) IS, ii, 1B, IC, lK and 1G; (10) 1S, 1E, IF and 1G; (11) 1S, 11, lB and 1J; (12) lS, 11, 1B, 1C, 1K, 1P, IN and 10; (13) IS, 1E, IF, IP, IN and 10; (14) IS, 1E, IL, 1M, 1N and 10; (15) lS, lE, IL, 1Q and 10; (16) 1B, IC, 1K and iG; (17) 11, 1E, IF and 1G; (18) 11, lE, IL, iM, 1P and 1G; (19) lB and 1J; (20) 11, 1E, 1F, 1P, 1N and 10; (21) 11, lE, 1L, IM, 1N and 10; (22) 11, 1E, 1L, 1Q and 10; 25 (23) 3A, 3B, 3C, 3D, 3E and 3F; and (24) 3A, 3B, 3C, 3G and 3F, wherein lB is a 5 aminopentanoate reductase, wherein IC is a 5-aminopent-2-enoate aminotransferase, a 5 aminopent-2-enoate dehydrogenase or an amine oxidase, wherein ID is a 2-oxoadipate decarboxylase, wherein 1 E is a glutarate semialdehyde reductase, wherein 1F is a 5 hydroxyvalerate dehydrogenase, wherein 1G is a 5-hydroxypent-2-enoate dehydratase, 30 wherein 1I is a 5-aminopentanoate aminotransferase, a 5-aminopentanoate dehydrogenase or an amine oxidase, wherein 1J is a 5-aminopent-2-enoate deaminase, wherin 1K is a 5 hydroxypent-2-enoate reductase, wherein 1L is a 5-hydroxyvaleryl-CoA transferase or a 5 hydroxyvaleryl-CoA synthetase, wherein IM is a 5-hydroxypentanoyl-CoA 17 dehydrogenase, wherein iN is a 5-hydroxypent-2-enoyl-CoA dehydratase, wherein 10 is a 2,4-pentadienoyl-CoA transferase, a 2,4-pentadienoyl-CoA synthetase or a 2,4 pentadienoyl-CoA hydrolase, wherein 1P is a 5-hydroxypent-2-enoyl-CoA transferase or a 5-hydroxypent-2-enoyl-CoA synthetase, wherein 1Q is a 5-hydroxyvaleryl-CoA 5 dehydratase/dehydrogenase, wherein iS a glutaryl-CoA reductase, wherein 3A is a 3 oxopentanoyl-CoA thiolase or 3-oxopentanoyl-CoA synthase, wherein 3B is a 3 oxopentanoyl-CoA reductase, wherein 3C is a 3-hydroxypentanoyl-CoA dehydratase, wherein 3D is a pent-2-enoyl-CoA isomerase, wherein 3E is a pent-3-enoyl-CoA dehydrogenase, wherein 3F is a 2,4-pentadienoyl-CoA hydrolase, a 2,4-pentadienoyl-CoA 10 transferase or a 2,4-pentadienoyl-CoA synthetase, wherein 3G is a pent-2-enoyl-CoA dehydrogenase. In some aspects of the invention, the microbial organism can include two, three, four, five, six, seven, eight, nine or ten exogenous nucleic acids each encoding a 2,4-pentadienoate pathway enzyme. In some aspects of the invention, the microbial organism can include 15 exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(24) as described above. In some aspects, the at least one exogenous nucleic acid is a heterologous nucleic acid. In some aspects, the non-naturally occurring microbial organism is in a substantially anaerobic culture medium. In some embodiments, the invention provides a non-naturally occurring microbial 20 organism as described herein, wherein the non-naturally occuring microbial organism having a 2,4-pentadienoate pathway selected from (9)-(15) as described above further includes a glutaryl-CoA pathway having at least one exogenous nucleic acid encoding a glutaryl-CoA pathway enzyme expressed in a sufficient amount to produce glutaryl-CoA, the glutaryl-CoA pathway having a pathway selected from: an acetoacetyl-CoA thiolase or 25 an acetoacetyl-CoA synthase; an acetoacetyl-CoA reductase; a 3-hydroxybutyryl-CoA dehydratase; and a glutaryl-CoA dehydrogenase; or a 2-aminoadipate aminotransferase, a 2-aminoadipate dehydrogenase or a 2-aminoadipate amine oxidase; and a 2-oxoadipate dehydrogenase, a 2-oxoadipate:ferridoxin oxidoreductase or a 2-oxoadipate formate lyase. In some embodiments, the invention provides a non-naturally occurring microbial 30 organism as described herein, wherein the non-naturally occuring microbial organism having a 2,4-pentadienoate pathway selected from (16)-(22) as described above further includes a 5-aminopentanoate pathway having at least one exogenous nucleic acid encoding a 5-aminopentanoate pathway enzyme expressed in a sufficient amount to 18 produce 5-aminopentanoate, the 5-aminopentanoate pathway having a 2-aminoadipate decarboxylase; or a 2-aminoadipate decarboxylase and a 2-aminoadipate aminotransferase, a 2-aminoadipate dehydrogenase or a 2-aminoadipate amine oxidase. In some embodiments, the invention provides a non-naturally occurring microbial 5 organism as described herein, wherein the non-naturally occuring microbial organism having a 2,4-pentadienoate pathway selected from (1)-(8) as described above further includes a 2-oxoadipate pathway having an exogenous nucleic acid encoding a 2 oxoadipate pathway enzyme expressed in a sufficient amount to produce a 2-oxoadipate, the 2-oxoadipate pathway having a 2-aminoadipate aminotransferase, a 2-aminoadipate 10 dehydrogenase or a 2-aminoadipate amine oxidase. In some embodiments, the invention provides a non-naturally occurring microbial organism having a 2,4-pentadienoate pathway having at least one exogenous nucleic acid encoding a 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce 2,4-pentadienoate, wherein the 2,4-pentadienoate pathway includes a pathway as 15 described above, further having: (i) a reductive TCA pathway having at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein the at least one exogenous nucleic acid is selected from an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; (ii) a reductive TCA pathway having at least one exogenous nucleic acid encoding a reductive TCA pathway 20 enzyme, wherein the at least one exogenous nucleic acid is selected from a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, a phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or (iii) at least one exogenous nucleic acid encodes an enzyme selected from a CO dehydrogenase, an H2 hydrogenase, and combinations thereof. 25 In some aspects, the microbial organism having (i) as described above further includes an exogenous nucleic acid encoding an enzyme selected from a pyruvate:ferredoxin oxidoreductase, an aconitase, an isocitrate dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, an acetate kinase, a phosphotransacetylase, an acetyl-CoA synthetase, an NAD(P)H:ferredoxin 30 oxidoreductase, ferredoxin, and combinations thereof. In some aspects, the microbial organism having (ii) further includes an exogenous nucleic acid encoding an enzyme selected from an aconitase, an isocitrate dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, and combinations thereof.

19 In some aspects, the microbial organism having (i) as described above includes four exogenous nucleic acids encoding an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; wherein the microbial organism having (ii) as described above includes five exogenous nucleic acids encoding a 5 pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, a phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or wherein the microbial organism having (iii) as described above includes two exogenous nucleic acids encoding a CO dehydrogenase and an H2 hydrogenase. In some embodiments, the invention provides a method for producing 2,4-pentadienoate, 10 having culturing the non-naturally occurring microbial organism as described herein under conditions and for a sufficient period of time to produce 2,4-pentadienoate. In some embodiments, the invention provides a non-naturally occurring microbial organism, having a microbial organism having a butadiene pathway having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient 15 amount to produce butadiene, wherein the butadiene pathway includes a pathway shown in Figures 1, 3, 5, 6 and/or 12 selected from: (1) 1D, 11, 1B, IC, 1K, IG and IT; (2) 1D, 1E, IF, IG and IT; (3) ID, 1E, 1L, IM, IP, IG and IT; (4) ID, 11, 1B, 1J and IT; (5) 1D, 11, 1B, IC, 1K, IP, IN, 10 and IT; (6) 1D, 1E, iF, IP, IN, 10 and iT; (7) ID, 1E, IL, 1M, iN, 10 and iT; (8) ID, IE, IL, 1Q, 10 and iT; (9) 1D, 1E, IF, 1U and 1V; (10) 1D, 11, 20 IB, 1C, 1K, lU and 1V; (11) 1D, 1E, IL, IM, iP, lU and 1V; (12) ID, lE, IW and 1V; (13) ID, 11, 1B, 1C, 1K, IG, 6A and 6B; (14) ID, 1E, IF, 1G, 6A and 6B; (15) ID, IE, IL, 1M, IP, 1G, 6A and 6B; (16) 1D, 11, IB, 1J, 6A and 6B; (17) 1D, 11, 1B, IC, 1K, iP, IN, 10, 6A and 6B; (18) ID, IE, IF, IP, IN, 10, 6A and 6B; (19) ID, 1E, IL, IM, IN, 10, 6A and 6B; (20) 1D, 1E, IL, 1Q, 10, 6A and 6B; (21) 1D, 11, 1B, 1C, 1K, IG, 6H, 6E 25 and 6B; (22) ID, 1E, iF, IG, 6H, 6E and 6B; (23) ID, 1E, IL, IM, IP, IG, 6H, 6E and 6B; (24) 1D, 11, 1B, 1J, 6H, 6E and 6B; (25) 1D, 11, 1B, IC, 1K, lP, IN, 10, 6H, 6E and 6B; (26) ID, 1E, IF, IP, IN, 10, 6H, 6E and 6B; (27) ID, 1E, IL, IM, IN, 10, 6H, 6E and 6B; (28) ID, 1E, IL, 1Q, 10, 6H, 6E and 6B; (29) ID, 11, 1B, IC, 1K, IP, IN, 6C and 6B; (30) ID, 1E, IF, IP, iN, 6C and 6B; (31) ID, 1E, IL, IM, IN, 6C and 6B; (32) ID, 30 1E, IL, iQ, 6C and 6B; (33) ID, 1I, IB, IC, 1K, iP, IN, 6D, 6E and 6B; (34) ID, lE, IF, lP, iN, 6D, 6E and 6B; (35) ID, 1E, 1L, IM, IN, 6D, 6E and 6B; (36) ID, 1E, IL, 1Q, 6D, 6E and 6B; (37) ID, ii, iB, IC, 1K, IG, 6F, 6C and 6B; (38) ID, 1E, IF, IG, 6F, 6C and 6B; (39) ID, IE, IL, 1M, IP, IG, 6F, 6C and 6B; (40) ID, 11, 1B, IC, 1K, IG, 6F, 20 6D, 6E and 6B; (41) 1D, 1E, IF, IG, 6F, 6D, 6E and 6B; (42) 1D, 1E, IL, IM, IP, IG, 6F, 6D, 6E and 6B; (43) IS, 11, 1B, IC, 1K, iG and IT; (44) IS, IE, IF, iG and IT; (45) IS, 11, iB, 1J and IT; (46) IS, 11, 1B, IC, 1K, IP, IN, 10 and IT; (47) IS, 1E, IF, iP, IN, 10 and IT; (48) IS, lE, IL, IM, IN, 10 and IT; (49) 1S, 1E, IL, 1Q, 10 and IT; (50) IS, 5 1E, IF, 1U and IV; (51) 1S, 11, 1B, IC, 1K, 1U and IV; (52) IS, 1E, IL, IM, IP, 1U and 1V; (53) IS, 1E, 1W and 1V; (54) IS, 1I, iB, IC, 1K, iG, 6A and 6B; (55) IS, 1E, IF, iG, 6A and 6B; (56) IS, 11, 1B, 1J, 6A and 6B; (57) IS, 11, iB, IC, 1K, iP, IN, 10, 6A and 6B; (58) IS, 1E, IF, iP, IN, 10, 6A and 6B; (59) IS, lE, IL, IM, IN, 10, 6A and 6B; (60) IS, 1E, IL, 1Q, 10, 6A and 6B; (61) IS, 11, IB, IC, 1K, IG, 6H, 6E and 6B; 10 (62) IS, 1E, IF, IG, 6H, 6E and 6B; (63) IS, 11, IB, 1J, 6H, 6E and 6B; (64) IS, 11, IB, IC, 1K, iP, IN, 10, 6H, 6E and 6B; (65) IS, 1E, IF, IP, IN, 10, 6H, 6E and 6B; (66) IS, IE, IL, IM, IN, 10, 6H, 6E and 6B; (67) iS, 1E, IL, 1Q, 10, 6H, 6E and 6B; (68) 1S, 11, 1B, IC, 1K, iP, IN, 6C and 6B; (69) IS, lE, IF, IP, IN, 6C and 6B; (70) IS, IE, IL, IM, IN, 6C and 6B; (71) IS, lE, IL, IQ, 6C and 6B; (72) IS, 1I, IB, IC, 1K, IP, IN, 6D, 6E 15 and 6B; (73) IS, lE, IF, iP, IN, 6D, 6E and 6B; (74) IS, lE, IL, IM, IN, 6D, 6E and 6B; (75) iS, 1E, IL, 1Q, 6D, 6E and 6B; (76) 1S, II, 1B, IC, 1K, IG, 6F, 6C and 6B; (77) 1S, 1E, IF, IG, 6F, 6C and 6B; (78) 1S, 11, 1B, IC, 1K, IG, 6F, 6D, 6E and 6B; (79) 1S, 1E, IF, IG, 6F, 6D, 6E and 6B; (80) 1B, IC, 1K, IG and IT; (81) 11, 1E, IF, IG and IT; (82) II, lE, IL, IM, IP, I G and IT; (83) 1B, I J and IT; (84) 11, lE, IF, IP, IN, 10 and IT; 20 (85) 11, 1E, IL, IM, IN, 10 and IT; (86) I, lE, IL, 1Q, 10 and IT; (87) 1B, IC, 1K, IU and 1V; (88) 11, 1E, IF, 1U and IV; (89) 11, 1E, IL, IM, IP, IU and 1V; (90) 11, 1E, 1W and IV; (91) 1B, IC, 1K, iG, 6A and 6B; (92) 11, 1E, IF, IG, 6A and 6B; (93) 11, lE, IL, IM, IP, IG, 6A and 6B; (94) IB, IJ, 6A and 6B; (95) 11, 1E, IF, IP, IN, 10, 6A and 6B; (96) 11, IE, IL, IM, IN, 10, 6A and 6B; (97) 1I, lE, IL, IQ, 10, 6A and 6B; (98) 1B, 25 IC, 1K, IG, 6H, 6E and 6B; (99) 11, 1E, IF, IG, 6H, 6E and 6B; (100) 11, IE, IL, I.M, IP, IG, 6H, 6E and 6B; (101) IB, IJ, 6H, 6E and 6B; (102) 11, 1E, IF, IP, IN, 10, 6H, 6E and 6B; (103) 11, 1E, IL, IM, IN, 10, 6H, 6E and 6B; (104) 11, 1E, IL, IQ, 10, 6H, 6E and 6B; (105) 11, 1E, IF, IP, IN, 6C and 6B; (106) 11, 1E, IL, IM, IN, 6C and 6B; (107) 11, lE, IL, IQ, 6C and 6B; (108) ii, 1E, IF, IP, IN, 6D, 6E and 6B; (109) 11, 1E, 1L, IM, 30 1N, 6D, 6E and 6B; (110) ii, 1E, IL, IQ, 6D, 6E and 6B; (I11) 1B, IC, 1K, IG, 6F, 6C and 6B; (112) 11, lE, IF, IG, 6F, 6C and 6B; (113) 11, 1E, IL, IM, IP, IG, 6F, 6C and 6B; (114) 1B, IC, 1K, 1G, 6F, 6D, 6E and 6B; (115) 11, 1E, IF, IG, 6F, 6D, 6E and 6B; (116) 11, 1E, IL, IM, IP, 1G, 6F, 6D, 6E and 6B; (117) 3A, 3B, 3C, 3D, 3E, 3F and IT; (118) 3A, 3B, 3C, 3D, 3E, 3F, 6A and 6B; (119) 3A, 3B, 3C, 3D, 3E, 3F, 6H, 6E and 6B; 21 (120) 3A, 3B, 3C, 3D, 3E, 6C and 6B; (121) 3A, 3B, 3C, 3D, 3E, 6D, 6E and 6B; and (122) 3A, 3B, 3C, 3G, 3F and IT; (123) 3A, 3B, 3C, 3G, 3F, 6A and 6B; (124) 3A, 3B, 3C, 3G, 3F, 6H, 6E and 6B; (125) 3A, 3B, 3C, 3G, 6C and 6B; (126) 3A, 3B, 3C, 3G, 6D, 6E and 6B; (127) 5A, 5B, 5C, 5D and 5E; (128) 7A, 7J, 7R, 7AD, 7AH, 12A, 12B and 5 12C; (129) 7A, 7H, 7F, 7R, 7AD, 7AH, 12A, 12B and 12C; (130) 7A, 7H, 7Q, 7Z, 7AD, 7AH, 12A, 12B and 12C; (131) 7A, 7H, 7Q, 7AC, 7AG, 7AH, 12A, 12B and 12C; (132) 7A, 7D, 71, 7R, 7AD, 7AH, 12A, 12B and 12C; (133) 7A, 7D, 7E, 7F, 7R, 7AD, 7AH, 12A, 12B and 12C; (134) 7A, 7D, 7E, 7Q, 7Z, 7AD, 7AH, 12A, 12B and 12C; (135) 7A, 7D, 7E, 7Q, 7AC, 7AG, 7AH, 12A, 12B and 12C; (136) 7A, 7D, 7P, 7N, 7AD, 7AH, 12A, 10 12B and 12C; (137) 7A, 7D, 7P, 7Y, 7Z, 7AD, 7AH, 12A, 12B and 12C; (138) 7A, 7D, 7P, 7Y, 7AC, 7AG, 7AH, 12A, 12B and 12C; (139) 7A, 7D, 7P, 7AB, 7V, 7AH, 12A, 12B and 12C; (140) 7A, 7D, 7P, 7AB, 7AF, 7AG, 7AH, 12A, 12B and 12C; (141) 7A, 7B, 7M, 7AD, 7AH, 12A, 12B and 12C; (142) 7A, 7B, 7L, 7Z, 7AD, 7AH, 12A, 12B and 12C; (143) 7A, 7B, 7L, 7AC, 7AG, 7AH, 12A, 12B and 12C; (144) 7A, 7B, 7X, 7Y, 7Z, 15 7AD, 7AH, 12A, 12B and 12C; (145) 7A, 7B, 7X, 7Y, 7AC, 7AG, 7AH, 12A, 12B and 12C; (146) 7A, 7B, 7X, 7AB, 7V, 7AH, 12A, 12B and 12C; (147) 7A, 7B, 7X, 7AB, 7AF, 7AG, 7AH, 12A, 12B and 12C; (148) 7A, 7B, 7C, 7U, 7AH, 12A, 12B and 12C; (149) 7A, 7B, 7C, 7T, 7AG, 7AH, 12A, 12B and 12C; (150) 7A, 7B, 7C, 7AE, 7AF, 7AG, 7AH, 12A, 12B and 12C; (151) 7A, 7D, 7P, 7AB, 7W, 12A, 12B and 12C; (152) 7A, 7B, 7X, 20 7AB, 7W, 12A, 12B and 12C; (153) 7A, 7B, 7C, 7AE, 7W, 12A, 12B and 12C; (154) 7A, 7B, 7C, 7AE, 7V, 7AH; , 12A, 12B and 12C (155) 7A, 7J, 7R, 7AD, 7AH, 12D and 12C; (156) 7A, 7H, 7F, 7R, 7AD, 7AH, 12D and 12C; (157) 7A, 7H, 7Q, 7Z, 7AD, 7AH, 12D and 12C; (158) 7A, 7H, 7Q, 7AC, 7AG, 7AH, 12D and 12C; (159) 7A, 7D, 71, 7R, 7AD, 7AH, 12D and 12C; (160) 7A, 7D, 7E, 7F, 7R, 7AD, 7AH, 12D and 12C; (161) 7A, 7D, 25 7E, 7Q, 7Z, 7AD, 7AH, 12D and 12C; (164) 7A, 7D, 7E, 7Q, 7AC, 7AG, 7AH, 12D and 12C; (163) 7A, 7D, 7P, 7N, 7AD, 7AH, 12D and 12C; (164) 7A, 7D, 7P, 7Y, 7Z, 7AD, 7AH, 12D and 12C; (165) 7A, 7D, 7P, 7Y, 7AC, 7AG, 7AH, 12D and 12C; (166) 7A, 7D, 7P, 7AB, 7V, 7AH, 12D and 12C; (167) 7A, 7D, 7P, 7AB, 7AF, 7AG, 7AH, 12D and 12C; (168) 7A, 7B, 7M, 7AD, 7AH, 12D and 12C; (169) 7A, 7B, 7L, 7Z, 7AD, 7AH, 12D 30 and 12C; (170) 7A, 7B, 7L, 7AC, 7AG, 7AH, 12D and 12C; (171) 7A, 7B, 7X, 7Y, 7Z, 7AD, 7AH, 12D and 12C; (172) 7A, 7B, 7X, 7Y, 7AC, 7AG, 7AH, 12D and 12C; (173) 7A, 7B, 7X, 7AB, 7V, 7AH, 12D and 12C; (174) 7A, 7B, 7X, 7AB, 7AF, 7AG, 7AH, 12D and 12C; (175) 7A, 7B, 7C, 7U, 7AH, 12D and 12C; (176) 7A, 7B, 7C, 7T, 7AG, 7AH, 12D and 12C; (177) 7A, 7B, 7C, 7AE, 7AF, 7AG, 7AH, 12D and 12C; (178) 7A, 7D, 7P, 22 7AB, 7W, 12D and 12C; (179) 7A, 7B, 7X, 7AB, 7W, 12D and 12C; (180) 7A, 7B, 7C, 7AE, 7W, 12D and 12C; (181) 7A, 7B, 7C, 7AE, 7V, 7AH, 12D and 12C; (182) 71, 7R, 7AD, 7AH, 12A, 12B and 12C; (183) 7E, 7F, 7R, 7AD, 7AH, 12A, 12B and 12C; (184) 7E, 7Q, 7Z, 7AD, 7AH, 12A, 12B and 12C; (185) 7E, 7Q, 7AC, 7AG, 7AH, 12A, 12B 5 and 12C; (186) 7P, 7N, 7AD, 7AH, 12A, 12B and 12C; (187) 7P, 7Y, 7Z, 7AD, 7AH, 12A, 12B and 12C; (188) 7P, 7Y, 7AC, 7AG, 7AH, 12A, 12B and 12C; (189) 7P, 7AB, 7V, 7AH, 12A, 12B and 12C; (190) 7P, 7AB, 7AF, 7AG, 7AH, 12A, 12B and 12C; (191) 7P, 7AB, 7W, 12A, 12B and 12C; (192) 71, 7R, 7AD, 7AH, 12D and 12C; (193) 7E, 7F, 7R, 7AD, 7AH, 12D and 12C; (194) 7E, 7Q, 7Z, 7AD, 7AH, 12D and 12C; (195) 7E, 7Q, 10 7AC, 7AG, 7AH, 12D and 12C; (196) 7P, 7N, 7AD, 7AH, 12D and 12C; (197) 7P, 7Y, 7Z, 7AD, 7AH, 12D and 12C; (198) 7P, 7Y, 7AC, 7AG, 7AH, 12D and 12C; (199) 7P, 7AB, 7V, 7AH, 12D and 12C; (200) 7P, 7AB, 7AF, 7AG, 7AH, 12D and 12C; (201) 7P, 7AB, 7W, 12D and 12C, (202) 7AS, 71, 7R, 7AD, 7AH, 12A, 12B and 12C; (203) 7AS, 7E, 7F, 7R, 7AD, 7AH, 12A, 12B and 12C; (204) 7AS, 7E, 7Q, 7Z, 7AD, 7AH, 12A, 12B 15 and 12C; (205) 7AS, 7E, 7Q, 7AC, 7AG, 7AH, 12A, 12B and 12C; (206) 7AS, 7P, 7N, 7AD, 7AH, 12A, 12B and 12C; (207) 7AS, 7P, 7Y, 7Z, 7AD, 7AH, 12A, 12B and 12C; (208) 7AS, 7P, 7Y, 7AC, 7AG, 7AH, 12A, 12B and 12C; (209) 7AS, 7P, 7AB, 7V, 7AH, 12A, 12B and 12C; (210) 7AS, 7P, 7AB, 7AF, 7AG, 7AH, 12A, 12B and 12C; (211) 7AS, 7P, 7AB, 7W, 12A, 12B and 12C; (212) 7AS, 71, 7R, 7AD, 7AH, 12D and 12C; (213) 20 7AS, 7E, 7F, 7R, 7AD, 7AH, 12D and 12C; (214) 7AS, 7E,. 7Q, 7Z, 7AD, 7AH, 12D and 12C; (215) 7AS, 7E, 7Q, 7AC, 7AG, 7AH, 12D and 12C; (216) 7AS, 7P, 7N, 7AD, 7AH, 12D and 12C; (217) 7AS, 7P, 7Y, 7Z, 7AD, 7AH, 12D and 12C; (218) 7AS, 7P, 7Y, 7AC, 7AG, 7AH, 12D and 12C; (219) 7AS, 7P, 7AB, 7V, 7AH, 12D and 12C; (220) 7AS, 7P, 7AB, 7AF, 7AG, 7AH, 12D and 12C; and (221) 7AS, 7P, 7AB, 7W, 12D and 12C, 25 wherein 1 B is a 5-aminopentanoate reductase, a 5-aminopent-2-enoate aminotransferase, a 5-aminopent-2-enoate dehydrogenase or 5-aminopent-2-enoate amine oxidase, wherein ID is a 2-oxoadipate decarboxylase, wherein 1E is a glutarate semialdehyde reductase, wherein IF is a 5-hydroxyvalerate reductase, wherein IG is a 5-hydroxypent-2-enoate dehydratase, wherein 1I is a 5-aminopentanoate aminotransferase, a 5-aminopentanoate 30 dehydrogenase or a 5-aminopentanoate amine oxidase, wherein 1J is a 5-aminopent-4 enoate deaminase, wherein 1K is a 5-hydroxypent-2-enoate reductase, wherein IL is a 5 hydroxyvaleryl-CoA transferase or a 5-hydroxyvaleryl-CoA synthetase, wherein IM is a 5-hydroxypentanoyl-CoA dehydrogenase, wherin IN is a 5-hydroxypent-2-enoyl-CoA dehydratase, wherein 10 is a 2,4-pentadienoyl-CoA transferase, a 2,4-pentadienoyl-CoA 23 synthetase or a 2,4-pentadienoyl-CoA hydrolase, wherein IP is a 5-hydroxypent-2-enoyl CoA transferase or a 5-hydroxypent-2-enoyl-CoA synthetase, wherein in 1Q is a 5 hydroxyvaleryl-CoA dehydratase/dehydrogenase, wherein IS is a glutaryl-CoA reductase, wherein IT is a 2,4-pentadienoate decarboxylase, wherein IU is a 5-hydroxypent-2-enoate 5 decarboxylase, wherein 1V is a 3-buten-1-ol dehydratase, wherein 1W is a 5 hydroxyvalerate decarboxylase, wherein 3A is a 3-oxopentanoyl-CoA thiolase or a 3 oxopentanoyl-CoA synthase, wherein 3B is a 3-oxopentanoyl-CoA reductase, wherein 3C is a 3-hydroxypentanoyl-CoA dehydratase, wherein 3D is a pent-2-enoyl-CoA isomerase, wherein 3E is a pent-3-enoyl-CoA dehydrogenase, wherein 3F is a 2,4-pentadienoyl-CoA 10 hydrolase, a 2,4-pentadienoyl-CoA transferase or a 2,4-pentadienoyl-CoA synthetase, wherein 3G is a pent-2-enoyl-CoA dehydrogenase, wherein 5A is a 4-hydroxy-2 oxovalerate aldolase, wherein 5B is a 4-hydroxy-2-oxovalerate dehydratase, wherein 5C is a 2-oxopentenoate decarboxylase, wherein 5D is a 3-buten- 1-al reductase, wherein 5E is a 3-buten-1-ol dehydratase, wherein 6A is a 2,4-pentadienoate reductase (acid reducing), 15 wherein 6B is a penta-2,4-dienal decarbonylase, wherein 6C is a 2,4-pentadienoyl-CoA reductase (acid reducing), wherein 6D is a 2,4-pentadienoyl-CoA phosphotransferase, wherein 6E is a 2,4-pentadienoyl-phosphate reductase, wherein 6F is a 2,4-pentadienoyl CoA hydrolase, a 2,4-pentadienoyl-CoA transferase or a 2,4-pentadienoyl-CoA synthetase, wherein 6H is a 2,4-pentadienoate kinase, wherein 7A is a 3-ketoacyl-ACP 20 synthase, wherein 7B is an acetoacetyl-ACP reductase, wherein 7C is a 3-hydroxybutyryl ACP dehydratase, wherein 7D is an acetoacetyl-CoA:ACP transferase, wherein 7E is an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA transferase or an acetoacetyl-CoA synthetase, wherein 7F is an acetoacetate reductase (acid reducing), wherein 7H is an acetoacetyl-ACP thioesterase, wherein 71 is an acetoacetyl-CoA reductase (CoA 25 dependent, aldehyde forming), wherein 7J is an acetoacetyl-ACP reductase (aldehyde forming), wherein 7K is an acetoacetyl-CoA reductase (alcohol forming), wherein 7L is an 3-hydroxybutyryl-ACP thioesterase, wherein 7M is an 3-hydroxybutyryl-ACP reductase (aldehyde forming), wherein 7N is an 3-hydroxybutyryl-CoA reductase (aldehyde forming), wherein 70 is an 3-hydroxybutyryl-CoA reductase (alcohol forming), wherein 30 7P is an acetoacetyl-CoA reductase (ketone reducing), wherein 7Q is an acetoacetate reductase (ketone reducing), wherein 7R is a 3-oxobutyraldehyde reductase (ketone reducing), wherein 7T is a crotonyl-ACP thioesterase, wherein 7U is a crotonyl-ACP reductase (aldehyde forming), wherein 7V is a crotonyl-CoA reductase (aldehyde forming), wherein 7W is a crotonyl-CoA (alcohol forming), wherein 7X is a 3- 24 hydroxybutyryl-CoA:ACP transferase, wherein 7Y is a 3-hydroxybutyryl-CoA hydrolase, a 3-hydroxybutyryl-CoA transferase or a 3-hydroxybutyryl-CoA synthetase, wherein 7Z is a 3-hydroxybutyrate reductase, wherein 7AB is a 3-hydroxybutyryl-CoA dehydratase, wherein 7AC is a 3-hydroxybutyrate dehydratase, wherein 7AD is a 3 5 hydroxybutyraldehyde dehydratase, wherein 7AE is a crotonyl-CoA:ACP transferase, wherein 7AF is a crotonyl-CoA hydrolase, a crotonyl-CoA transferase or a crotonyl-CoA synthetase, wherein 7AG is a crotonate reductase, wherein 7AH is a crotonaldehyde reductase, wherein 7AS is an acetoacetyl-CoA synthase, wherein 12A is a crotyl alcohol kinase, wherein 12B is a 2-butenyl-4-phosphate kinase, wherein 12C is a butadiene 10 synthase, and wherein 12D is a crotyl alcohol diphosphokinase. In some aspects of the invention, the microbial organism can include two, three, four, five, six, seven, eight, nine, ten or eleven exogenous nucleic acids each encoding a butadiene pathway enzyme. In some aspects, the microbial organism includes exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1) 15 (221). In some aspects, the at least one exogenous nucleic acid is a heterologous nucleic acid. In some aspects, the non-naturally occurring microbial organism is in a substantially anaerobic culture medium. In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein the non-naturally occuring microbial organism 20 having a butadiene pathway selected from (43)-(79) as described above further includes a glutaryl-CoA pathway having at least one exogenous nucleic acid encoding a glutaryl CoA pathway enzyme expressed in a sufficient amount to produce glutaryl-CoA, the glutaryl-CoA pathway having a pathway selected from: an acetoacetyl-CoA thiolase or acetoacetyl-CoA synthase; an acetoacetyl-CoA reductase; a 3-hydroxybutyryl-CoA 25 dehydratase; and a glutaryl-CoA dehydrogenase; or a 2-aminoadipate aminotransferase, a 2-aminoadipate dehydrogenase or a 2-aminoadipate amine oxidase; and a 2-oxoadipate dehydrogenase, a 2-oxoadipate:ferridoxin oxidoreductase or a 2-oxoadipate formate lyase. In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein the non-naturally occuring microbial organism 30 having a butadiene pathway selected from (80)-(116) as described above further includes a 5-aminopentanoate pathway having at least one exogenous nucleic acid encoding a 5 aminopentanoate pathway enzyme expressed in a sufficient amount to produce 5 aminopentanoate, the 5-aminopentanoate pathway having a 2-aminoadipate 25 decarboxylase; or a 2-aminoadipate decarboxylase and a 2-aminoadipate aminotransferase, a 2-aminoadipate dehydrogenase or a 2-aminoadipate amine oxidase. In some embodiments, the invention provides a non-naturally occurring microbial organism as described herein, wherein the non-naturally occuring microbial organism 5 having a butadiene pathway selected from (1)-(42) as described above further includes a 2 oxoadipate pathway having an exogenous nucleic acid encoding a 2-oxoadipate pathway enzyme expressed in a sufficient amount to produce a 2-oxoadipate, the 2-oxoadipate pathway having a 2-aminoadipate aminotransferase, a 2-aminoadipate dehydrogenase or a 2-aminoadipate amine oxidase. 10 In some embodiments, the invention provides a non-naturally occurring microbial organism having a butadiene pathway having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce butadiene, wherein the butadiene pathway includes a pathway as described above, further having: (i) a reductive TCA pathway having at least one exogenous nucleic acid encoding a reductive 15 TCA pathway enzyme, wherein the at least one exogenous nucleic acid is selected from an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha ketoglutarate:ferredoxin oxidoreductase; (ii) a reductive TCA pathway having at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein the at least one exogenous nucleic acid is selected from a pyruvate:ferredoxin oxidoreductase, a 20 phosphoenolpyruvate carboxylase, a phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or (iii) at least one exogenous nucleic acid encodes an enzyme selected from a CO dehydrogenase, an H2 hydrogenase, and combinations thereof. In some aspects, the microbial organism having (i) as described above further includes an 25 exogenous nucleic acid encoding an enzyme selected from a pyruvate:ferredoxin oxidoreductase, an aconitase, an isocitrate dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, an acetate kinase, a phosphotransacetylase, an acetyl-CoA synthetase, an NAD(P)H:ferredoxin oxidoreductase, ferredoxin, and combinations thereof. In some aspect, the microbial 30 organism having (ii) as described above further includes an exogenous nucleic acid encoding an enzyme selected from an aconitase, an isocitrate dehydrogenase, a succinyl CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, and combinations thereof. In some aspects, the microbial organism having (i) as described 26 above includes four exogenous nucleic acids encoding an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; wherein the microbial organism having (ii) as described above includes five exogenous nucleic acids encoding a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, a 5 phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or wherein the microbial organism having (iii) as described above includes two exogenous nucleic acids encoding a CO dehydrogenase and an H2 hydrogenase. In some embodiments, the invention provides a method for producing butadiene, having culturing the non-naturally occurring microbial organism as described herein under 10 conditions and for a sufficient period of time to produce butadiene. In some embodiments, the invention provides a non-naturally occurring microbial organism, having a microbial organism having a 1,3-butanediol pathway having at least one exogenous nucleic acid encoding a 1,3-butanediol pathway enzyme expressed in a sufficient amount to produce 1,3-butanediol, wherein the 1,3-butanediol pathway includes 15 a pathway shown in Figures 4, 5 and/or 7 selected from: (1) 4A, 4B, 4C and 4D; (2) 5A, 5H, 5J, 5K and 5G; (3) 5A, 5H, 51 and 5G; (4) 5A, 5H and 5L; (5) 5A, 5F and 5G; (6) 7A, 7D, 7E, 7F, 7G and 7S; (7) 7A, 7D, 71, 7G and 7S; (8) 7A, 7D, 7K, and 7S; (9) 7A, 7H, 7F, 7G and 7S; (10) 7A, 7J, 7G and 7S; (11) 7A, 7J, 7R and 7AA; (12) 7A, 7H, 7F, 7R and 7AA; (13) 7A, 7H, 7Q, 7Z and 7AA; (14) 7A, 7D, 71, 7R and 7AA; (15) 7A, 7D, 7E, 20 7F, 7R and 7AA; (16) 7A, 7D, 7E, 7Q, 7Z and 7AA; (17) 7A, 7D, 7P, 7N and 7AA; (18) 7A, 7D, 7P, 7Y, 7Z and 7AA; (19) 7A, 7B, 7M and 7AA; (20) 7A, 7B, 7L, 7Z and 7AA; (21) 7A, 7B, 7X, 7N and 7AA; (22) 7A, 7B, 7X, 7Y, 7Z and 7AA; (23) 7A, 7D, 7P and 70; (24) 7A, 7B, 7X and 70; (25) 7A, 7D, 7E, 7F, 7R, 7AA; (26) 7A, 7D, 7E, 7F, 7G, 7S, (27) 7AS, 7E, 7F, 7G and 7S; (28) 7AS, 71, 7G and 7S; (29) 7AS, 7K, and 7S; (30) 7AS, 25 71, 7R and 7AA; (31) 7AS, 7E, 7F, 7R and 7AA; (32) 7AS, 7E, 7Q, 7Z and 7AA; (33) 7AS, 7P, 7N and 7AA; (34) 7AS, 7P, 7Y, 7Z and 7AA; (35) 7AS, 7P and 70; (36) 7AS, 7E, 7F, 7R, and 7AA; and (37) 7AS, 7E, 7F, 7G, and 7S, wherein 4A is a 3-oxo-5 hydroxypentanoyl-CoA thiolase or a 3-oxo-5-hydroxypentanoyl-CoA synthase, wherein 4B is a 3-oxo-5-hydroxypentanoyl-CoA hydrolase, 3-oxo-5-hydroxypentanoyl-CoA 30 transferase or 3-oxo-5-hydroxypentanoyl-CoA synthetase, wherein 4C is a 3-oxo-5 hydroxypentanoate decarboxylase, wherein 4D is a 3-oxobutanol reductase, wherein in 5A is a 4-hydroxy-2-oxovalerate aldolase, wherein 5F is a 4-hydroxy-2-oxovalerate decarboxylase, wherin 5G is a 3-hydroxybutanal reductase, wherein 5H is a 4-hydroxy-2- 27 oxopentanoate dehydrogenase, a 4-hydroxy-2-oxopentanoate:ferredoxin oxidoreductase or a 4-hydroxy-2-oxopentanoate formate lyase, wherein 51 is a 3-hydroxybutyryl-CoA reductase (aldehyde forming), wherein 5J is a 3-hydroxybutyryl-CoA hydrolase, a 3 hydroxybutyryl-CoA transferase or a 3-hydroxybutyryl-CoA synthetase, wherein 5K is a 5 3-hydroxybutyrate reductase, wherein 5L is a 3-hydroxybutyryl-CoA reductase (alcohol forming), wherein 7A is a 3-ketoacyl-ACP synthase, wherein 7B is an acetoacetyl-ACP reductase, wherein 7D is an acetoacetyl-CoA:ACP transferase, wherein 7E is an acetoacetyl-CoA hydrolase, acetoacetyl-CoA transferase or acetoacetyl-CoA synthetase, wherein 7F is an acetoacetate reductase (acid reducing), wherein 7G is a 3 10 oxobutyraldehyde reductase (aldehyde reducing), wherein 7H is an acetoacetyl-ACP thioesterase, wherein 71 is an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), wherein 7J is an acetoacetyl-ACP reductase (aldehyde forming), wherein 7K is an acetoacetyl-CoA reductase (alcohol forming), wherein 7L is a 3-hydroxybutyryl-ACP thioesterase, wherein 7M is a 3-hydroxybutyryl-ACP reductase (aldehyde forming), 15 wherein 7N is a 3-hydroxybutyryl-CoA reductase (aldehyde forming), wherein 70 is a 3 hydroxybutyryl-CoA reductase (alcohol forming), wherein 7P is an acetoacetyl-CoA reductase (ketone reducing), wherein 7Q is an acetoacetate reductase (ketone reducing), wherein 7R is a 3-oxobutyraldehyde reductase (ketone reducing), wherein 7S is a 4 hydroxy-2-butanone reductase, wherein 7X is a 3-hydroxybutyryl-CoA:ACP transferase, 20 wherein 7Y is a 3-hydroxybutyryl-CoA hydrolase, a 3-hydroxybutyryl-CoA transferase or a 3-hydroxybutyryl-CoA synthetase, wherein 7Z is a 3-hydroxybutyrate reductase, wherein 7AA is a 3-hydroxybutyraldehyde reductase and wherein 7AS is an acetoacetyl CoA synthase. In some aspects, the microbial organism includes two, three, four or five exogenous 25 nucleic acids each encoding a 1,3-butanediol pathway enzyme. In some aspects, the microbial organism includes exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(37) as described above. In some aspects, the at least one exogenous nucleic acid is a heterologous nucleic acid. In some aspects, the non-naturally occurring microbial organism is in a substantially anaerobic culture medium. 30 In some embodiments, the invention provides a non-naturally occurring microbial organism having a 1,3-butanediol pathway having at least one exogenous nucleic acid encoding a 1,3-butanediol pathway enzyme expressed in a sufficient amount to produce 1,3-butanediol, wherein the 1,3-butanediol pathway includes a pathway as described 28 above, further having: (i) a reductive TCA pathway having at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein the at least one exogenous nucleic acid is selected from an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; (ii) a reductive TCA pathway having at 5 least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein the at least one exogenous nucleic acid is selected from a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, a phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or (iii) at least one exogenous nucleic acid encodes an enzyme selected from a CO dehydrogenase, an H2 hydrogenase, and 10 combinations thereof. In some aspects, the microbial organism having (i) as described above further includes an exogenous nucleic acid encoding an enzyme selected from a pyruvate:ferredoxin oxidoreductase, an aconitase, an isocitrate dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, an acetate kinase, a 15 phosphotransacetylase, an acetyl-CoA synthetase, an NAD(P)H:ferredoxin oxidoreductase, ferredoxin, and combinations thereof. In some aspects, the microbial organism having (ii) as described above further includes an exogenous nucleic acid encoding an enzyme selected from an aconitase, an isocitrate dehydrogenase, a succinyl CoA synthetase, a succinyl-CoA transferase, a fumarase, a palate dehydrogenase, and 20 combinations thereof. In some aspects, the microbial organism having (i) as described herein includes four exogenous nucleic acids encoding an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; wherein the microbial organism having (ii) includes five exogenous nucleic acids encoding a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, a 25 phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or wherein the microbial organism having (iii) includes two exogenous nucleic acids encoding a CO dehydrogenase and an H2 hydrogenase. In some embodiments, the invention provides a method for producing 1,3-butanediol, having culturing the non-naturally occurring microbial organism as described herein under 30 conditions and for a sufficient period of time to produce 1,3-butanediol. In some embodiments, the invention provides a non-naturally occurring microbial organism, having a microbial organism having a 3-buten-1-ol pathway having at least one exogenous nucleic acid encoding a 3-buten-1-ol pathway enzyme expressed in a sufficient 29 amount to produce 3-buten-1-ol, wherein the 3-buten-1-ol pathway includes a pathway shown in Figures 1 and/or 5 selected from: (1) 1D, IE, IF and IU; (2) 1D, 11, 1B, IC, 1K and 1U; (3) ID, lE, IL, IM, IP and lU; (4) ID, lE and 1W; (5) IS, lE, iF and 1U; (6) IS, 1I, IB, IC, 1K and 1U; (7) IS, lE, IL, IM, iP and 1U; (8) IS, lE and 1W; (9) IB, 5 IC, 1K and 1U; (10) 11, lE, IF and 1U; (11) 11, 1E, IL, IM, iP and 1U; (12) 11, 1E and 1W; and (13) 5A, 5B, 5C and 5D, wherein lB is a 5-aminopentanoate reductase, wherein IC is a 5-aminopent-2-enoate aminotransferase, a 5-aminopent-2-enoate dehydrogenase or an amine oxidase, wherein ID is a 2-oxoadipate decarboxylase, wherein 1E is a glutarate semialdehyde reductase, wherein IF is a 5-hydroxyvalerate dehydrogenase, wherein 11 is a 10 5-aminopentanoate aminotransferase, a 5-aminopentanoate dehydrogenase or a 5 aminopentanoate amine oxidase, wherein 1K is a 5-hydroxypent-2-enoate reductase, wherein IL is a 5-hydroxyvaleryl-CoA transferase or a 5-hydroxyvaleryl-CoA synthetase, wherein IM is a 5-hydroxypentanoyl-CoA dehydrogenase, wherein IP is a 5-hydroxypent 2-enoyl-CoA transferase or a 5-hydroxypent-2-enoyl-CoA synthetase, wherein IS is a 15 glutaryl-CoA reductase, wherein 1U is a 5-hydroxypent-2-enoate decarboxylase, wherein 1W is a 5-hydroxyvalerate decarboxylase, wherein 5A is a 4-hydroxy-2-oxovalerate aldolase, wherein 5B is a 4-hydroxy-2-oxovalerate dehydratase, wherein 5C is a 2 oxopentenoate decarboxylase, wherein 5D is a 3-buten- 1-al reductase. In some aspects, the microbial organism includes two, three, four, five or six exogenous 20 nucleic acids each encoding a 3-buten-I -ol pathway enzyme. In some aspects, the microbial organism includes exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(13) as described above. In some aspects, the at least one exogenous nucleic acid is a heterologous nucleic acid. In some aspects, the non-naturally occurring microbial organism is in a substantially anaerobic culture medium. 25 In some embodiments, the invention provides a non-naturally occurring microbial organism having a 3-buten-1-ol pathway having at least one exogenous nucleic acid encoding a 3-buten-1-ol pathway enzyme expressed in a sufficient amount to produce 3 buten-I-ol, wherein the 3-buten-1-ol pathway includes a pathway as described above, further having: (i) a reductive TCA pathway having at least one exogenous nucleic acid 30 encoding a reductive TCA pathway enzyme, wherein the at least one exogenous nucleic acid is selected from an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; (ii) a reductive TCA pathway having at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein the 30 at least one exogenous nucleic acid is selected from a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, a phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or (iii) at least one exogenous nucleic acid encodes an enzyme selected from a CO dehydrogenase, an H2 hydrogenase, and 5 combinations thereof. In some aspects, the microbial organism having (i) as described above further includes an exogenous nucleic acid encoding an enzyme selected from a pyruvate:ferredoxin oxidoreductase, an aconitase, an isocitrate dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, an acetate kinase, a .10 phosphotransacetylase, an acetyl-CoA synthetase, an NAD(P)H:ferredoxin oxidoreductase, ferredoxin, and combinations thereof In some aspects, the microbial organism having (ii) as described above further includes an exogenous nucleic acid encoding an enzyme selected from an aconitase, an isocitrate dehydrogenase, a succinyl CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, and 15 combinations thereof. In some aspects the microbial organism having (i) as described above includes four exogenous nucleic acids encoding an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; wherein the microbial organism having (ii) as described above includes five exogenous nucleic acids encoding a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, a 20 phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or wherein the microbial organism having (iii) as described above includes two exogenous nucleic acids encoding a CO dehydrogenase and an H2 hydrogenase. In some embodiments, the invention provides a method for producing 3-buten-1-ol, having culturing the non-naturally occurring microbial organism as described above under 25 conditions and for a sufficient period of time to produce 3-buten-1-ol. In some embodiments, the invention provides a method for producing butadiene, having culturing the non-naturally occurring microbial organism as described above under conditions and for a sufficient to produce 3-buten-1-ol, and chemically dehydrating the 3 buten-1-ol to produce butadiene. 30 In some embodiments, the invention provides a non-naturally occurring microbial organism, including a microbial organism having a crotyl alcohol pathway including at least one exogenous nucleic acid encoding a crotyl alcohol pathway enzyme expressed in a 31 sufficient amount to produce crotyl alcohol, wherein the crotyl alcohol pathway includes a pathway shown in Figure 7 selected from: (1) 7A, 7J, 7R, 7AD and 7AH; (2) 7A, 7H, 7F, 7R, 7AD and 7AH; (3) 7A, 7H, 7Q, 7Z, 7AD and 7AH; (4) 7A, 7H, 7Q, 7AC, 7AG and 7AH; (5) 7A, 7D, 71, 7R, 7AD and 7AH; (6) 7A, 7D, 7E, 7F, 7R, 7AD and 7AH; (7) 7A, 5 7D, 7E, 7Q, 7Z, 7AD and 7AH; (8) 7A, 7D, 7E, 7Q, 7AC, 7AG and 7AH; (9) 7A, 7D, 7P, 7N, 7AD and 7AH; (10) 7A, 7D, 7P, 7Y, 7Z, 7AD and 7AH; (11) 7A, 7D, 7P, 7Y, 7AC, 7AG and 7AH; (12) 7A, 7D, 7P, 7AB, 7V and 7AH; (13) 7A, 7D, 7P, 7AB, 7AF, 7AG AND 7AH (14) 7A, 7B, 7M, 7AD and 7AH; (15) 7A, 7B, 7L, 7Z, 7AD and 7AH; (16) 7A, 7B, 7L, 7AC, 7AG and 7AH; (17) 7A, 7B, 7X, 7Y, 7Z, 7AD and 7AH; (18) 7A, 7B, 10 7X, 7Y, 7AC, 7AG and 7AH; (19) 7A, 7B, 7X, 7AB, 7V and 7AH; (20) 7A, 7B, 7X, 7AB, 7AF, 7AG and 7AH; (21) 7A, 7B, 7C, 7U and 7AH; (22) 7A, 7B, 7C, 7T, 7AG and 7AH; (23) 7A, 7B, 7C, 7AE, 7AF, 7AG and 7AH; (24) 7A, 7D, 7P, 7AB and 7W; (25) 7A, 7B, 7X, 7AB and 7W; (26) 7A, 7B, 7C, 7AE and 7W; (27) 7A, 7B, 7C, 7AE, 7V and 7AH; (28) 71, 7R, 7AD and 7AH; (29) 7E, 7F, 7R, 7AD and 7AH; (30) 7E, 7Q, 7Z, 7AD 15 and 7AH; (31) 7E, 7Q, 7AC, 7AG and 7AH; (32) 7P, 7N, 7AD and 7AH; (33) 7P, 7Y, 7Z, 7AD and 7AH; (34) 7P, 7Y, 7AC, 7AG and 7AH; (35) 7P, 7AB, 7V and 7AH; (36) 7P, 7AB, 7AF, 7AG and 7AH; (37) 7P, 7AB and 7W, (38) 7AS, 71, 7R, 7AD and 7AH; (39) 7AS, 7E, 7F, 7R, 7AD and 7AH; (40) 7AS, 7E, 7Q, 7Z, 7AD and 7AH; (41) 7AS, 7E, 7Q, 7AC, 7AG and 7AH; (42) 7AS, 7P, 7N, 7AD and 7AH; (43) 7AS, 7P, 7Y, 7Z, 7AD and 20 7AH; (44) 7AS, 7P, 7Y, 7AC, 7AG and 7AH; (45) 7AS, 7P, 7AB, 7V and 7AH; (46) 7AS, 7P, 7AB, 7AF, 7AG and 7AH; and (47) 7AS, 7P, 7AB and 7W, wherein 7A is a 3 ketoacyl-ACP synthase, wherein 7B is an acetoacetyl-ACP reductase, wherein 7C is a 3 hydroxybutyryl-ACP dehydratase, wherein 7D is an acetoacetyl-CoA:ACP transferase, wherein 7E is an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA transferase or an 25 acetoacetyl-CoA synthetase, wherein 7F is an acetoacetate reductase (acid reducing), wherein 7H is an acetoacetyl-ACP thioesterase, wherein 71 is an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), wherein 7J is an acetoacetyl-ACP reductase (aldehyde forming), wherein 7K is an acetoacetyl-CoA reductase (alcohol forming), wherein 7L is an 3-hydroxybutyryl-ACP thioesterase, wherein 7M is an 3 30 hydroxybutyryl-ACP reductase (aldehyde forming), wherein 7N is an 3-hydroxybutyryl CoA reductase (aldehyde forming), wherein 70 is an 3-hydroxybutyryl-CoA reductase (alcohol forming), wherein 7P is an acetoacetyl-CoA reductase (ketone reducing), wherein 7Q is an acetoacetate reductase (ketone reducing), wherein 7R is a 3-oxobutyraldehyde reductase (ketone reducing), wherein 7T is a crotonyl-ACP thioesterase, wherein 7U is a 32 crotonyl-ACP reductase (aldehyde forming), wherein 7V is a crotonyl-CoA reductase (aldehyde forming), wherein 7W is a crotonyl-CoA (alcohol forming), wherein 7X is a 3 hydroxybutyryl-CoA:ACP transferase, wherein 7Y is a 3-hydroxybutyryl-CoA hydrolase, a 3-hydroxybutyryl-CoA transferase or a 3-hydroxybutyryl-CoA synthetase, wherein 7Z is 5 a 3-hydroxybutyrate reductase, wherein 7AB is a 3-hydroxybutyryl-CoA dehydratase, wherein 7AC is a 3-hydroxybutyrate dehydratase, wherein 7AD is a 3 hydroxybutyraldehyde dehydratase, wherein 7AE is a crotonyl-CoA:ACP transferase, wherein 7AF is a crotonyl-CoA hydrolase, a crotonyl-CoA transferase or a crotonyl-CoA synthetase, wherein 7AG is a crotonate reductase, wherein 7AH is a crotonaldehyde 10 reductase and wherein 7AS is an acetoacetyl-CoA synthase. In some aspects, the invention provides that the microbial organism having a crotyl alcohol pathway as described above, wherein the microbial organism includes two, three, four, five, six or seven exogenous nucleic acids each encoding a crotyl alcohol pathway enzyme. In some aspects, the microbial organism includes exogenous nucleic acids 15 encoding each of the enzymes of at least one of the crotyl alcohol pathways selected from (I)-(47) as described above. In some aspects, the at least one exogenous nucleic acid is a heterologous nucleic acid, In som aspects, the non-naturally occurring microbial organism is in a substantially anaerobic culture medium. In some embodiments, the invention provides a non-naturally occurring microbial 20 organism having a crotyl alcohol pathway having at least one exogenous nucleic acid encoding a crotyl alcohol pathway enzyme expressed in a sufficient amount to produce crotyl alcohol, wherein the crotyl alcohol pathway includes a pathway as described above, and further includes: (i) a reductive TCA pathway including at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein the at least one 25 exogenous nucleic acid is selected from an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; (ii) a reductive TCA pathway including at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein the at least one exogenous nucleic acid is selected from a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, a 30 phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or (iii) at least one exogenous nucleic acid encodes an enzyme selected from a CO dehydrogenase, an H2 hydrogenase, and combinations thereof.

33 In some aspects, the microbial organism including (i) as described above further includes an exogenous nucleic acid encoding an enzyme selected from a pyruvate:ferredoxin oxidoreductase, an aconitase, an isocitrate dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, an acetate kinase, a 5 phosphotransacetylase, an acetyl-CoA synthetase, an NAD(P)H:ferredoxin oxidoreductase, ferredoxin, and combinations thereof. In some aspects, the microbial organism including (ii) as described above further includes an exogenous nucleic acid encoding an enzyme selected from an aconitase, an isocitrate dehydrogenase, a succinyl CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, and 10 combinations thereof. In some aspects, the microbial organism including (i) as described above includes four exogenous nucleic acids encoding an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; wherein the microbial organism including (ii) includes five exogenous nucleic acids encoding a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, a 15 phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or wherein the microbial organism including (iii) includes two exogenous nucleic acids encoding a CO dehydrogenase and an H2 hydrogenase. In some embodiments, the invention provides a method for producing crotyl alcohol, including culturing the non-naturally occurring microbial organism as described above 20 under conditions and for a sufficient period of time to produce crotyl alcohol. In some embodiments, access to butadiene can be accomplished by biosynthetic production of crotyl alcohol and subsequent chemical dehydration to butadiene. In some embodiments, the invention provides a process for the production of butadiene that includes (a) culturing by fermentation in a sufficient amount of nutrients and media a non 25 naturally occurring microbial organism as described herein that produces crotyl alcohol; and (b) converting crotyl alcohol produced by culturing the non-naturally occurring microbial organism to butadiene. The dehydration of alcohols are known in the art and can include various thermal processes, both catalyzed and non-catalyzed. In some embodiments, a catalyzed thermal 30 dehydration employs a metal oxide catalyst or silica. In some embodiments, step (b) of the process is performed by chemical dehydration in the presence of a catalyst. For example, it has been indicated that crotyl alcohol can be dehydrated over bismuth molybdate (Adams, C.R. J. Catal. 10:355-361, 1968) to afford 1,3-butadiene.

34 Dehydration can be achieved via activation of the alcohol group and subsequent elimination by standard elimination mechanisms such as El or E2 elimination. Activation can be achieved by way of conversion of the alcohol group to a halogen such as iodide, chloride, or bromide. Activation can also be accomplished by way of a sulfonyl, 5 phosphate or other activating functionality that convert the alcohol into a good leaving group. In some embodiments, the activating group is a sulfate or sulfate ester selected from a tosylate, a mesylate, a nosylate, a brosylate, and a triflate. In some embodiments, the leaving group is a phosphate or phosphate ester. In some such embodiments, the dehydrating agent is phosphorus pentoxide. 10 In some embodiments, the invention provides a non-naturally occurring microbial organism, including a microbial organism having a propylene pathway including at least one exogenous nucleic acid encoding a propylene pathway enzyme expressed in a sufficient amount to produce propylene, wherein the propylene pathway includes a pathway shown in Figure 7 selected from: (1) 7A, 7J, 7R, 7AD and 7AO; (2) 7A, 7H, 7F, 15 7R, 7AD and 7AO; (3) 7A, 7D, 71, 7R, 7AD and 7AO; (4) 7A, 7D, 7E, 7F, 7R, 7AD and 7AO; (5) 7A, 7H, 7Q, 7Z, 7AD and 7AO; (6) 7A, 7D, 7E, 7Q, 7AD and 7AO; (7) 7A, 7D, 7P, 7Y, 7Z, 7AD and 7AO; (8) 7A, 7D, 7P, 7N, 7AD and 7AO; (9) 7A, 7B, 7X, 7N, 7AD and 7AO; (10) 7A, 7B, 7X, 7Y, 7Z, 7AD and 7AO; (11) 7A, 7H, 7Q, 7V, 7AG and 7AO; (12) 7A, 7D, 7E, 7Q, 7AC, 7AG and 7AO; (13) 7A, 7D, 7P, 7Y, 7AC, 7AG and 20 7AO; (14) 7A, 7D, 7P, 7AB, 7AF, 7AG and AO; (15) 7A, 7P, 7AB, 7V and 7AO; (16) 7A, 7B, 7M, 7AD and 7AO; (17) 7A, 7B, 7L, 7Z, 7AD and 7AO; (18) 7A, 7B, 7X, 7N, 7AD and 7AO; (19) 7A, 7B, 7X, 7Y, 7Z, 7AD and 7AO; (20) 7A, 7B, 7C, 7U and 7AO; (21) 7A, 7B, 7C, 7T, 7AG and 7AO; (22) 7A, 7B, 7C, 7AE, 7V and 7AO; (23) 7A, 7B, 7C, 7AE, 7AF, 7AG and 7AO; (24) 7A, 7H, 7Q and 7AR; (25) 7A, 7D, 7E, 7Q and 25 7AR; (26) 7A, 7D, 7P, 7Y and 7AR; (27) 7A, 7B, 7X, 7Y and 7AR; (28) 7A, 7B, 7L and 7AR; (29) 7A, 7H, 7Q, 7AC and 7AQ; (30) 7A, 7D, 7E, 7Q, 7AC and 7AQ;(31) 7A, 7D, 7P, 7Y, 7AC and 7AQ; (32) 7A, 7D, 7P, 7AB, 7AF and 7AQ; (33) 7A, 7B, 7L, 7AC and 7AQ; (34) 7A, 7B, 7X, 7Y, 7AC and 7AQ; (35) 7A, 7B, 7X, 7AB, 7AF and 7AQ; (36) 7A, 7B, 7C, 7AE, 7AF and 7AQ; (37) 7A, 7B, 7C, 7T and 7AQ; (38) 7A, 7H, 7Q, 30 7AC, 7AN and 7AK; (39) 7A, 7D, 7E, 7Q, 7AC, 7AN and 7AK; (40) 7A, 7D, 7P, 7Y, 7AC, 7AN and 7AK; (41) 7A, 7D, 7P, 7AB, 7AF, 7AN and 7AK; (42) 7A, 7D, 7P, 7AB, 7AM, 7AJ and 7AK; (43) 7A, 7B, 7L, 7AC, 7AN and 7AK; (44) 7A, 7B, 7X, 7Y, 7AC, 7AN and 7AK; (45) 7A, 7B, 7X, 7AB, 7AF, 7AN and 7AK; (46) 7A, 7B, 7X, 7AB, 7AM, 7AJ and 7AK; (47) 7A, 7B, 7C, 7T, 7AN and 7AK; (48) 7A, 7B, 7C, 7AE, 7AF, 35 7AN and 7AK; (49) 7A, 7B, 7C, 7AE, 7AM, 7AJ and 7AK; (50) 7A, 7B, 7C, 7AL, 7AP and 7AK; (51) 7A, 7B, 7C, 7AL, 7AI, 7AJ and 7AK; (52) 7A, 7B, 7X, 7AB, 7V and 7AO; (53) 7A 7B, 7L, 7AC, 7AG and 7AO; (54) 7A, 7B, 7X, 7Y, 7AC, 7AC, 7AG and 7AO; (55) 7A, 7B, 7X, 7AB, 7AF, 7AG and 7AO; and (56) 7A, 7H, 7Q, 7AC, 7AG and 5 7AO; (57) 71, 7R, 7AD and 7AO; (58) 7E, 7F, 7R, 7AD and 7AO; (59) 7E, 7Q, 7Z, 7AD and 7AO; (60) 7P, 7Y, 7Z, 7AD and 7AO; (61) 7P, 7N, 7AD and 7AO; (62) 7E, 7Q, 7AC, 7AG and 7AO; (63) 7P, 7Y, 7AC, 7AG and 7AO; (64) 7P, 7AB, 7AF, 7AG and 7AO; (65) 7P, 7AB, 7V and 7AO; (66) 7E, 7Q and 7AR; (67) 7P, 7Y and 7AR; (68) 7E, 7Q, 7AC and 7AQ; (69) 7P, 7Y, 7AC and 7AQ; (70) 7P, 7AB, 7AF and 7AQ; (71) 7E, 7Q, 10 7AC, 7AN and 7AK; (72) 7P, 7Y, 7AC, 7AN and 7AK; (73) 7P, 7AB, 7AF, 7AN and 7AK; (74) 7P, 7AB, 7AM, 7AJ and 7AK, (75) 7AS, 71, 7R, 7AD and 7AO; (76) 7AS, 7E, 7F, 7R, 7AD and 7AO; (77) 7AS, 7E, 7Q, 7AD and 7AO; (78) 7AS, 7P, 7Y, 7Z, 7AD and 7AO; (79) 7AS, 7P, 7N, 7AD and 7AO; (80) 7AS, 7E, 7Q, 7AC, 7AG and 7AO; (81) 7AS, 7P, 7Y, 7AC, 7AG and 7AO; (82) 7AS, 7P, 7AB, 7AF, 7AG and 7AO; (83) 7AS, 15 7E, 7Q and 7AR; (84) 7AS, 7P, 7Y and 7AR; (85) 7AS, 7E, 7Q, 7AC and 7AQ; (86) 7AS, 7P, 7Y, 7AC and 7AQ; (87) 7AS, 7P, 7AB, 7AF and 7AQ; (88) 7AS, 7E, 7Q, 7AC, 7AN and 7AK; (89) 7AS, 7P, 7Y, 7AC, 7AN and 7AK;(90) 7AS, 7P, 7AB, 7AF, 7AN and 7AK; and (91) 7AS, 7P, 7AB, 7AM, 7AJ and 7AK, wherein 7A is a 3-ketoacyl-ACP synthase, wherein 7B is an acetoacetyl-ACP reductase, wherein 7C is a 3-hydroxybutyryl 20 ACP dehydratase, wherein 7D is an acetoacetyl-CoA:ACP transferase, wherein 7E is an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA transferase or an acetoacetyl-CoA synthetase, wherein 7F is an acetoacetate reductase (acid reducing), wherein 7H is an acetoacetyl-ACP thioesterase, wherein 71 is an acetoacetyl-CoA reductase (CoA dependent, aldehyde forming), wherein 7J is an acetoacetyl-ACP reductase (aldehyde 25 forming), wherein 7L is a 3-hydroxybutyryl-ACP thioesterase, wherein 7M is a 3 hydroxybutyryl-ACP reductase (aldehyde forming), wherein 7N is a 3-hydroxybutyryl CoA reductase (aldehyde forming), wherein 7P is an acetoacetyl-CoA reductase (ketone reducing), wherein 7Q is an acetoacetate reductase (ketone reducing), wherein 7R is a 3 oxobutyraldehyde reductase (ketone reducing), wherein 7S is a 4-hydroxy-2-butanone 30 reductase, wherein 7T is a crotonyl-ACP thioesterase, wherein 7U is a crotonyl-ACP reductase (aldehyde forming), wherein 7V is a crotonyl-CoA reductase (aldehyde forming), wherein 7X is a 3-hydroxybutyryl-CoA:ACP transferase, wherein 7Y is a 3 hydroxybutyryl-CoA hydrolase, a 3-hydroxybutyryl-CoA transferase or a 3 hydroxybutyryl-CoA synthetase, wherein 7Z is a 3-hydroxybutyrate reductase, wherein 36 7AB is a 3-hydroxybutyryl-CoA dehydratase, wherein 7AC is a 3-hydroxybutyrate dehydratase, wherein 7AD is a 3-hydroxybutyraldehyde dehydratase, wherein 7AE is a crotonyl-CoA:ACP transferase, wherein 7AF is a crotonyl-CoA hydrolase, a crotonyl-CoA transferase or a crotonyl-CoA synthetase, wherein 7AG is a crotonate reductase, wherein 5 7AI is a butryl-CoA:ACP transferase, wherein 7AJ is a butyryl-CoA transferase, a butyryl CoA hydrolase or a butyryl-CoA synthetase, wherein 7AK is a butyrate decarboxylase, wherein 7AL is a crotonyl-ACP reductase, wherein 7AM is a crotonyl-CoA reductase, wherein 7AN is a crotonate reductase, wherein 7AO is a crotonaldehyde decarbonylase, wherein 7AP is a butyryl-ACP thioesterase, wherein 7AQ is a crotonate decarboxylase, 10 wherein 7AR is a 3-hydroxybutyrate decarboxylase and wherein 7AS is an acetoacetyl CoA synthase. In some aspects, the invention provides that the microbial organism having a propylene pathway as described above, wherein the microbial organism includes two, three, four, five, six, seven or eight exogenous nucleic acids each encoding a propylene pathway 15 enzyme. In some aspects, the microbial organism includes exogenous nucleic acids encoding each of the enzymes of at least one of the pathways selected from (1)-(91) as described above. In some aspects, the at least one exogenous nucleic acid is a heterologous nucleic acid. In some aspects, the non-naturally occurring microbial organism is in a substantially anaerobic culture medium. 20 In some embodiments, the invention provides a non-naturally occurring microbial organism having a propylene pathway having at least one exogenous nucleic acid encoding a propylene pathway enzyme expressed in a sufficient amount to produce propylene, wherein the propylene pathway includes a pathway as described above, and further includes: (i) a reductive TCA pathway including at least one exogenous nucleic 25 acid encoding a reductive TCA pathway enzyme, wherein the at least one exogenous nucleic acid is selected from an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; (ii) a reductive TCA pathway including at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein the at least one exogenous nucleic acid is selected from a pyruvate:ferredoxin 30 oxidoreductase, a phosphoenolpyruvate carboxylase, a phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or (iii) at least one exogenous nucleic acid encodes an enzyme selected from a CO dehydrogenase, an H2 hydrogenase, and combinations thereof.

37 In some aspects, the microbial organism including (i) as described above further includes an exogenous nucleic acid encoding an enzyme selected from a pyruvate:ferredoxin oxidoreductase, an aconitase, an isocitrate dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, an acetate kinase, a 5 phosphotransacetylase, an acetyl-CoA synthetase, an NAD(P)H:ferredoxin oxidoreductase, ferredoxin, and combinations thereof. In some aspects, the microbial organism including (ii) as described above further includes an exogenous nucleic acid encoding an enzyme selected from an aconitase, an isocitrate dehydrogeriase, a succinyl CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, and 10 combinations thereof. In some aspects, the microbial organism including (i) as described above includes four exogenous nucleic acids encoding an ATP-citrate lyase, citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; wherein the microbial organism including (ii) as described above includes five exogenous nucleic acids encoding a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, 15 a phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H2 hydrogenase; or wherein the microbial organism including (iii) as described above includes two exogenous nucleic acids encoding a CO dehydrogenase and an H2 hydrogenase. In some embodiments, the invention provides a method for producing propylene, including culturing the non-naturally occurring microbial organism of as described above 20 under conditions and for a sufficient period of time to produce propylene. In an additional embodiment, the invention provides a non-naturally occurring microbial organism having a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway, wherein the non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme or protein that converts a 25 substrate to a product selected from the group consisting of 2-aminoadipate to 5 aminopentanoate, 2-aminoadipate to 2-oxoadipate, 5-aminopentanoate to glutarate semialdehyde, 2-oxoadipate to glutarate semialdehyde, 2-oxoadipate to glutaryl-CoA, glutaryl-CoA to glutarate semialdehyde, glutarate semialdehyde to 5-hydroxyvalerate, 5 aminopentanoate to 5-aminopent-2enoate, 5-aminopent-2enoate to 5-hydroxypent-2 30 enoate, 5-hydroxypent-2-enoate to 5-hydroxypent-2-enoate, 5-hydroxyvalerate to 5 hydroxypent-2-enoate, 5-hydroxyvalerate to 5-hydroxyvaleryl-CoA, 5-hydroxyvalerate to 3-buten- 1 -ol, 5-aminopent-2-enoate to 2,4-pentadienoate, 5-hydroxypent-2-enoate to 3 buten-1-ol, 5-hydroxypent-2-enoate to 2,4-pentadienoate, 5-hydroxypent-2-enoate to 5- 38 hydroxypent-2-enoyl-CoA, 5-hydroxypent-2-enoyl-CoA to 5-hydroxypent-2-enoate, glutarate semialdehyde to 5-aminopentanoate, 2-oxoadipate to 2-aminoadipate, 5 hydroxyvaleryl-CoA to 5-hydroxypent-2-enoyl-CoA, 5-hydroxyvaleryl-CoA to 2,4 pentadienoyl-CoA, 5-hydroxypent-2-enoyl-CoA to 2,4-pentadienoyl-CoA, 2,4 5 pentadienoyl-CoA to 2,4-pentadienoate, 2,4-pendienoate to butadiene, 3-buten-1-ol to butadiene, acetyl-CoA to acetoacetyl-CoA, acetoacetyl-CoA to 3-hydroxybutyryl-CoA, 3 hydroxybutyryl-CoA to crotonyl-CoA, crotonyl-CoA, glutaryl-CoA, propionyl-CoA and acetyl-CoA to 3-oxopentanoyl-CoA, propionyl-CoA and malonyl-CoA to 3-oxopentanoyl CoA, 3-oxopentanoyl-CoA to 3-hydroxypentanoyl-CoA, 3-hydroxypentanoyl-CoA to 10 pent-2-onoyl-CoA, pent-2-onoyl-CoA to pent-3-enoyl-CoA, pent-3-enoyl-CoA to 2,4 pentadienoyl-CoA, 3-hydroxypropionyl-CoA and acetyl-CoA to 3-oxo-5 hydroxypentanoyl-CoA, 3-oxo-5-hydroxypentanoyl-CoA to 3-oxo-5-hydroxypentanoate, 3-oxo-5-hydroxypentanoate to 3-oxobutanol, 3-oxobutanol to 1,3-butanediol, pyruvate and acetaldehyde to 4-hydroxy-2-oxovalerate, 4-hydroxy-2-oxovalerate to 2-oxopentenoate, 2 15 oxopentenoate to 3-buten-1-al, 3-buten-1-al to 3-buten-1-ol, 4-hydroxy-2-oxovalerate to 3 hydroxybutyryl-CoA, 4-hydroxy-2-oxovalerate to 3-hydroxybutanal, 3-hydroxybutyryl CoA to 3-hydroxybutanal, 3-hydroxybutanal to 1,3-butanediol, 3-hydroxybutyryl-CoA to 3-hydroxybutyrate, 3-hydroxybutyrate to 3-hydroxybutyrate to 3-hydroxybutanal, 3 hydroxybutyryl-CoA to 1,3-butanediol,'2,4-pentadienoate to 2,4-pentadienoyl-CoA, 2,4 20 pentadienoate to penta-2,4-dienal, penta-2,4-dienal to butadiene, 2,4-pentadienoate to 2,4 pentadienoyl-phosphate, 2,4-pentadienoyl-phosphate to penta-2,4-dienal, 2,4 pentadienoyl-CoA to 2,4-pentadienoyl-phosphate, 2,4-pentadienoyl-CoA to penta-2,4 dienal, malonyl-ACP and acetyl-CoA or acetyl-ACP to acetoacetyl-ACP, acetoacetyl-ACP to 3-hydroxybutyryl-ACP, 3-hydroxybutyryl-ACP to crotonyl-ACP, acetoacetyl-ACP to 25 acetoacctyl-CoA, malonyl-CoA and acetyl-CoA to acetoacetyl-CoA, acetoacetyl-CoA to acetoacetate, acetoacetate to 3-oxobutyraldehyde, 3-oxobutyraldehyde to 4-hydroxy-2 butanone, acetoacetyl-ACP to acetoacetate, acetoacetyl-CoA to 3-oxobutyraldehyde, acetoacetyl-ACP to 3-oxobutyraldehyde, acetoacetyl-CoA to 4-hydroxy-2-butanone, 3 hydroxybutyryl-ACP to 3-hydroxybutyrate, 3-hydroxybutyryl-ACP to 3 30 hydroxybutyraldehyde, 3-hydroxybutyryl-CoA to 3-hydroxybutyraldehyde, 3 hydroxybutyryl-CoA to 1,3-butanediol, acetoacetyl-CoA to 3-hydroxybutyryl-CoA, acetoacetate to 3-hydroxybutyrate, 3-oxobutyraldehyde to 3-hydroxybutyraldehyde, 4 hydroxy-2-butanone to 1,3-butanediol, crotonyl-ACP to crotonate, crotonyl-ACP to crotonaldehyde, crotonyl-CoA to crotonaldehyde, crotonyl-CoA to crotyl alcohol, 3- 39 hydroxybutyryl-ACP to 3-hydroxybutyryl-CoA, 3-hydroxybutyryl-CoA to 3 hydroxybutyrate, 3-hydroxybutyrate to 3-hydroxybutyraldehyde, 3-hydroxybutyraldehyde to 1,3-butanediol, 3-hydroxybutyryl-CoA to crotonyl-CoA, 3-hydroxybutyrate to crotonate, 3-hydroxybutyraldehyde to crotonaldehyde, crotonyl-ACP to crotonyl-CoA, 5 crotonyl-CoA to crotonate, crotonate to crotonaldehyde, crotonaldehyde to crotyl alcohol, butyryl-ACP to butyryl-CoA, butyryl-CoA to butyrate, butyrate to propylene, crotonyl ACP to butyryl-ACP, crotonyl-CoA to butyryl-CoA, crotonate to butyrate, crotonaldehyde to propylene, butyryl-ACP to butyrate, crotonate to propylene, 3-hydroxybutyrate to propylene, crotyl alcohol to 2-butenyl-4-phosphate, 2-butenyl-4-phosphate to 2-butenyl-4 10 diphosphate, crotyl alcohol to 2-butenyl-4-diphosphate and 2-butenyl-4-diphosphate to butadiene. One skilled in the art will understand that these are merely exemplary and that any of the substrate-product pairs disclosed herein suitable to produce a desired product and for which an appropriate activity is available for the conversion of the substrate to the product can be readily determined by one skilled in the art based on the teachings herein. 15 Thus, the invention provides a non-naturally occurring microbial organism containing at least one exogenous nucleic acid encoding an enzyme or protein, where the enzyme or protein converts the substrates and products of a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol pathway, such as that shown in Figures 1-7 and 12. 20 While generally described herein as a microbial organism that contains a 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway, it is understood that the invention additionally provides a non-naturally occurring microbial organism having at least one exogenous nucleic acid encoding a 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol 25 pathway enzyme expressed in a sufficient amount to produce an intermediate of a 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway. For example, as disclosed herein, a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway is exemplified in Figures 1-7 and 12. Therefore, in addition to a microbial organism containing a 2,4-pentadienoate, 30 butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol pathway that produces 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol, the invention additionally provides a non-naturally occurring microbial organism having at least one exogenous nucleic acid encoding a 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten-1-ol pathway enzyme, where the microbial organism 40 produces a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3 buten- 1 -ol pathway intermediate, for example, 5-aminopent-2-enoate, glutarate semialdehyde, 5-hydroxyvalerate, 5-hydroxyvaleryl-CoA, 5-hydroxypent-2-enoyl-CoA, 2,4-pentadienoyl-CoA, 5-hydroxypent-2-enoate, 5-hydroxypent-2-enoate, acetoacetyl 5 CoA, 3-hydroxybutyryl-CoA, crotoyl-CoA, glutaryl-CoA, 3-oxopentanoyl-CoA, 3 hydroxypentanoyl-CoA, pent-2-enoyl-CoA, pent-3-enoyl-CoA, 3-oxo-5 hydroxypentanoyl-CoA, 3-oxo-5-hydroxypentanoate, 3-oxobutanol, 4-hydroxy-2 oxovalerate, 2-oxopentenoate, 3-buten-1-al, 3-hydroxybutyryl-CoA, 3-hydroxybutyrate, 3 hydroxybutanal, 2,4-pentadienoyl-phosphate, penta-2,4-dienal, acetoacetyl-ACP, 10 acetoacetyl-CoA, acetoacetate, 3-oxobutyraldehyde, 4-hydroxy-2-butanone, 3 hydroxybutyryl-ACP, 3-hydroxybutyryl-CoA, 3-hydroxybutyrate, 3 hydroxybutyraldehyde, crotonyl-ACP, crotonyl-CoA, crotonate, crotonaldehyde, butyryl ACP, butyryl-CoA, butyrate, 2-butenyl-4-phosphate, or 2-butenyl-4-diphosphate. It is understood that any of the pathways disclosed herein, as described in the Examples 15 and exemplified in the Figures, including the pathways of Figures 1-9 and 12, can be utilized to generate a non-naturally occurring microbial organism that produces any pathway intermediate or product, as desired. As disclosed herein, such a microbial organism that produces an intermediate can be used in combination with another microbial organism expressing downstream pathway enzymes to produce a desired product. 20 However, it is understood that a non-naturally occurring microbial organism that produces a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1-ol pathway intermediate can be utilized to produce the intermediate as a desired product. The invention is described herein with general reference to the metabolic reaction, reactant or product thereof, or with specific reference to one or more nucleic acids or genes 25 encoding an enzyme associated with or catalyzing, or a protein associated with, the referenced metabolic reaction, reactant or product. Unless otherwise expressly stated herein, those skilled in the art will understand that reference to a reaction also constitutes reference to the reactants and products of the reaction. Similarly, unless otherwise expressly stated herein, reference to a reactant or product also references the reaction, and 30 reference to any of these metabolic constituents also references the gene or genes encoding the enzymes that catalyze or proteins involved in the referenced reaction, reactant or product. Likewise, given the well known fields of metabolic biochemistry, enzymology and genomics, reference herein to a gene or encoding nucleic acid also constitutes a 41 reference to the corresponding encoded enzyme and the reaction it catalyzes or a protein associated with the reaction as well as the reactants and products of the reaction. As disclosed herein, the product 2,4-pentadienoate and intermediates 5-aminopentanoate, 5-aminopent-2-enoate, 5-hydroxypent-2-enoate, 5-hydroxyvalerate, 5-hydroxypent-2 5 enoate, 3-oxo-5-hydroxypentanoate, 3-hydroxybutyrate, 4-hydroxy-2-exovalerate, 2 oxopentenoate, acetoacetate, crotonate, butyrate, as well as other intermediates, are carboxylic acids, which can occur in various ionized forms, including fully protonated, partially protonated, and fully deprotonated forms. Accordingly, the suffix "-ate," or the acid form, can be used interchangeably to describe both the free acid form as well as any 10 deprotonated form, in particular since the ionized form is known to depend on the pH in which the compound is found. It is understood that carboxylate products or intermediates includes ester forms of carboxylate products or pathway intermediates, such as 0 carboxylate and S-carboxylate esters. 0- and S-carboxylates can include lower alkyl, that is C1 to C6, branched or straight chain carboxylates. Some such 0- or S-carboxylates 15 include, without limitation, methyl, ethyl, n-propyl, n-butyl, i-propyl, sec-butyl, and tert butyl, pentyl, hexyl 0- or S-carboxylates, any of which can further possess an unsaturation, providing for example, propenyl, butenyl, pentyl, and hexenyl 0- or S carboxylates. O-carboxylates can be the product of a biosynthetic pathway. Exemplary 0-carboxylates accessed via biosynthetic pathways can include, without limitation, methyl 20 2,4-pentadienoate, ethyl 2,4-pentadienoate, and n-propyl 2,4-pentadienoate. Other biosynthetically accessible O-carboxylates can include medium to long chain groups, that is C7-C22, O-carboxylate esters derived from fatty alcohols, such heptyl, octyl, nonyl, decyl, undecyl, lauryl, tridecyl, myristyl, pentadecyl, cetyl, palmitolyl, heptadecyl, stearyl, nonadecyl, arachidyl, heneicosyl, and behenyl alcohols, any one of which can be 25 optionally branched and/or contain unsaturations. O-carboxylate esters can also be accessed via a biochemical or chemical process, such as esterification of a free carboxylic acid product or transesterification of an 0- or S-carboxylate. S-carboxylates are exemplified by CoA S-esters, cysteinyl S-esters, alkylthioesters, and various aryl and heteroaryl thioesters. The non-naturally occurring microbial organisms of the invention 30 can be produced by introducing expressible nucleic acids encoding. one or more of the enzymes or proteins participating in one or more 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol biosynthetic pathways. Depending on the host microbial organism chosen for biosynthesis, nucleic acids for some or all of a particular 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3- 42 buten- 1-ol biosynthetic pathway can be expressed. For example, if a chosen host is deficient in one or more enzymes or proteins for a desired biosynthetic pathway, then expressible nucleic acids for the deficient enzyme(s) or protein(s) are introduced into the host for subsequent exogenous expression. Alternatively, if the chosen host exhibits 5 endogenous expression of some pathway genes, but is deficient in others, then an encoding nucleic acid is needed for the deficient enzyme(s) or protein(s) to achieve 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol biosynthesis. Thus, a non-naturally occurring microbial organism of the invention can be produced by introducing exogenous enzyme or protein activities to obtain a desired 10 biosynthetic pathway or a desired biosynthetic pathway can be obtained by introducing one or more exogenous enzyme or protein activities that, together with one or more endogenous enzymes or proteins, produces a desired product such as 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol. Host microbial organisms can be selected from, and the non-naturally occurring microbial 15 organisms generated in, for example, bacteria, yeast, fungus or any of a variety of other microorganisms applicable to fermentation processes. Exemplary bacteria include species selected from Escherichia coli, Klebsiella oxytoca, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Rhizobium etli, Bacillus subtilis, Corynebacterium glutamicum, Cupriavidus necator, 20 Gluconobacter oxydans, Zymamonas mobilis, Lactococcus lactis, Lactobacillus plantarum, Streptomyces coelicolor, Clostridium acetobutylicum, Pseudomonas fluorescens, and Pseudomonas putida. Exemplary yeasts or fungi include species selected from Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Pichia pastoris, 25 Rhizopus arrhizus, Rhizobus oryzae, Yarrowia lipolytica, Candida albicans and the like. E. coli is a particularly useful host organism since it is a well characterized microbial organism suitable for genetic engineering. Other particularly useful host organisms include yeast such as Saccharomyces cerevisiae. It is understood that any suitable microbial host organism can be used to introduce metabolic and/or genetic modifications 30 to produce a desired product. Depending on the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol biosynthetic pathway constituents of a selected host microbial organism, the non-naturally occurring microbial organisms of the invention will include at least one 43 exogenously expressed 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway-encoding nucleic acid and up to all encoding nucleic acids for one or more 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol biosynthetic pathways. For example, 2,4-pentadienoate, 5 butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol biosynthesis can be established in a host deficient in a pathway enzyme or protein through exogenous expression of the corresponding encoding nucleic acid. In a host deficient in all enzymes or proteins of a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway, exogenous expression of all enzymes or proteins in the pathway can 10 be included, although it is understood that all enzymes or proteins of a pathway can be expressed even if the host contains at least one of the pathway enzymes or proteins. For example, exogenous expression of all enzymes or proteins in a pathway for production of butadiene can be included, such as, a glutaryl-CoA reductase, aglutarate semialdehyde reductase, a 5-hydroxyvaleryl-CoA transferase and/or synthetase, a 5-hydroxyvaleryl-CoA 15 dehydratase/dehydrogenase, a 2,4-pentadienoyl-CoA transferase, synthetase or hydrolase and a 2,4-pentadienoate decarboxylase. As another example, exogenous expression of all enzymes or proteins in a pathway for production of 1,3-butanediol can be included, such as, a 3-ketoacyl-ACP synthase, an acetoacetyl-ACP reductase, a 3-hydroxybutyryl CoA:ACP transferase, an 3-hydroxybutyryl-CoA hydrolase, transferase or synthetase, a 3 20 hydroxybutyrate reductase, and a 3-hydroxybutyraldehyde reductase. As yet another example, exogenous expression of all enzymes or proteins in a pathway for production of crotyl-alcohol can be included, such as, a 3-ketoacyl-ACP synthase, an acetoacetyl-ACP reductase, a 3-hydroxybutyryl-ACP dehydratase, a crotonyl-CoA:ACP transferase, a crotonyl-CoA hydrolase, transferase or synthetase, a crotonate reductase and a 25 crotonaldehyde reductase. Given the teachings and guidance provided herein, those skilled in the art will understand that the number of encoding nucleic acids to introduce in an expressible form will, at least, parallel the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3 buten- 1 -ol pathway deficiencies of the selected host microbial organism. Therefore, a 30 non-naturally occurring microbial organism of -the invention can have one, two, three, four, five, six, seven, eight, nine, ten or eleven, up to all nucleic acids encoding the enzymes or proteins constituting a 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten- 1 -ol biosynthetic pathway disclosed herein. In some embodiments, the non-naturally occurring microbial organisms also can include other 44 genetic modifications that facilitate or optimize 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol biosynthesis or that confer other useful functions onto the host microbial organism. One such other functionality can include, for example, augmentation of the synthesis of one or more of the 2,4-pentadienoate, 5 butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol pathway precursors such as 2-aminoadipate, 5-aminopentanoate, 2-oxoadipate, glutaryl-CoA, propionyl-CoA, acetyl-CoA, malonyl-CoA, 3-hydroxypropionyl-CoA, malonyl-ACP or pyruvate. Generally, a host microbial organism is selected such that it produces the precursor of a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol 10 pathway, either as a naturally produced molecule or as an engineered product that either provides de novo production of a desired precursor or increased production of a precursor naturally produced by the host microbial organism. For example, 2-aminoadipate, 5 aminopentanoate, 2-oxoadipate, glutaryl-CoA, propionyl-CoA, acetyl-CoA, malonyl-CoA, 3-hydroxypropionyl-CoA, pyruvate or malonyl-ACP is produced naturally in a host 15 organism such as E. coli. A host organism can be engineered to increase production of a precursor, as disclosed herein. In addition, a microbial organism that has been engineered to produce a desired precursor can be used as a host organism and further engineered to express enzymes or proteins of a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway. 20 In some embodiments, a non-naturally occurring microbial organism of the invention is generated from a host that contains the enzymatic capability to synthesize 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. In this specific embodiment it can be useful to increase the synthesis or accumulation of a 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol 25 pathway product to, for example, drive 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten-1-ol pathway reactions toward 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol production. Increased synthesis or accumulation can be accomplished by, for example, overexpression of nucleic acids encoding one or more of the above-described 2,4-pentadienoate, butadiene, 30 propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway enzymes or proteins. Overexpression of the enzyme or enzymes and/or protein or proteins of the 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1-ol pathway can occur, for example, through exogenous expression of the endogenous gene or 45 genes, or through exogenous expression of the heterologous gene or genes. Therefore, naturally occurring organisms can be readily generated to be non-naturally occurring microbial organisms of the invention, for example, producing 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol, through 5 overexpression of one, two, three, four, five, six, seven, eight, nine, ten or eleven, that is, up to all nucleic acids encoding 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol biosynthetic pathway enzymes or proteins. In addition, a non-naturally occurring organism can be generated by mutagenesis of an endogenous gene that results in an increase in activity of an enzyme in the 2,4-pentadienoate, butadiene, 10 propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol biosynthetic pathway. In particularly useful embodiments, exogenous expression of the encoding nucleic acids is employed. Exogenous expression confers the ability to custom tailor the expression and/or regulatory elements to the host and application to achieve a desired expression level that is controlled by the user. However, endogenous expression also can be utilized in 15 other embodiments such as by removing a negative regulatory effector or induction of the gene's promoter when linked to an inducible promoter or other regulatory element. Thus, an endogenous gene having a naturally occurring inducible promoter can be up-regulated by providing the appropriate inducing agent, or the regulatory region of an endogenous gene can be engineered to incorporate an inducible regulatory element, thereby allowing 20 the regulation of increased expression of an endogenous gene at a desired time. Similarly, an inducible promoter can be included as a regulatory element for an exogenous gene introduced into a non-naturally occurring microbial organism. It is understood that, in methods of the invention, any of the one or more exogenous nucleic acids can be introduced into a microbial organism to produce a non-naturally 25 occurring microbial organism of the invention. The nucleic acids can be introduced so as to confer, for example, a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol biosynthetic pathway onto the microbial organism. Alternatively, encoding nucleic acids can be introduced to produce an intermediate microbial organism having the biosynthetic capability to catalyze some of the required reactions to confer 2,4 30 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol biosynthetic capability. For example, a non-naturally occurring microbial organism having a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3 buten- 1 -ol biosynthetic pathway can comprise at least two exogenous nucleic acids 46 encoding desired enzymes or proteins, such as the combination of a 5-hydroxypentanoyl CoA dehydrogenase and a 2,4-pentadienoyl-CoA transferase, or alternatively a 5 aminopentanoate reductase and a 5-aminopent-2-enoate deaminase, or alternatively a 2,4 pentadienoate decarboxylase and a 3-hydroxybutyryl-CoA dehydratase, or alternatively a 5 3-oxopentanoyl-CoA reductase and a 2,4-pentadienoyl-CoA hydrolase, or alternatively a 4-hydroxy-2-oxopentanoate dehydrogenase and a 3-hydroxybutyryl-CoA reductase (alcohol forming), or alternatively a 3-hydroxybutyrate reductase and a 3 hydroxybutyraldehyde reductase, and the like. Thus, it is understood that any combination of two or more enzymes or proteins of a biosynthetic pathway can be included in a non 10 naturally occurring microbial organism of the invention. Similarly, it is understood that any combination of three or more enzymes or proteins of a biosynthetic pathway can be included in a non-naturally occurring microbial organism of the invention, for example, a 5-hydroxyvalerate dehydrogenase, a 5-hydroxypent-2-enoyl-CoA transferase and a 5 hydroxypent-2-enoyl-CoA dehydratase, or alternavely a penta-2,4-dienal decarbonylase, a 15 2,4-pentadienoyl-CoA reductase (acid reducing) and a 5-hydroxyvaleryl-CoA dehydratase/dehydrogenase, or alternatively a 2-oxopentenoate decarboxylase, a 3-buten 1-al reductase and a 3-buten-1-ol dehydratase, or alternatively a crotonaldehyde reductase, a crotonate reductase and a crotonyl-CoA hydrolase, and so forth, as desired, so long as the combination of enzymes and/or proteins of the desired biosynthetic pathway results in 20 production of the corresponding desired product. Similarly, any combination of four, five, six, seven, eight, nine, ten, eleven or more enzymes or proteins of a biosynthetic pathway as disclosed herein can be included in a non-naturally occurring microbial organism of the invention, as desired, so long as the combination of enzymes and/or proteins of the desired biosynthetic pathway results in production of the corresponding desired product. 25 In addition to the biosynthesis of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol as described herein, the non-naturally occurring microbial organisms and methods of the invention also can be utilized in various combinations with each other and with other microbial organisms and methods well known in the art to achieve product biosynthesis by other routes. For example, one alternative to produce 2,4 30 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol other than use of the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol producers is through addition of another microbial organism capable of converting a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3 buten-1-ol pathway intermediate to 2,4-pentadienoate, butadiene, propylene, 1,3- 47 butanediol, crotyl alcohol or 3-buten-1-ol. One such procedure includes, fbr example, the fermentation of a microbial organism that produces a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway intermediate. The 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol 5 pathway intermediate can then be used as a substrate for a second microbial organism that converts the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3 buten-1-ol pathway intermediate to 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten-1-ol. The 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol pathway intermediate can be added directly 10 to another culture of the second organism or the original culture of the 2,4-pentadienoate, butadiene, propylene, 1,3 -butanediol, crotyl alcohol or 3-buten- 1 -ol pathway intermediate producers can be depleted of these microbial organisms by, for example, cell separation, and then subsequent addition of the second organism to the fermentation broth can be utilized to produce the final product without intermediate purification steps. 15 In other embodiments, the non-naturally occurring microbial organisms and methods of the invention can be assembled in a wide variety of subpathways to achieve biosynthesis of, for example, 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. In these embodiments, biosynthetic pathways for a desired product of the invention can be segregated into different microbial organisms, and the different microbial 20 organisms can be co-cultured to produce the final product. In such a biosynthetic scheme, the product of one microbial organism is the substrate for a second microbial organism until the final product is synthesized. For example, the biosynthesis of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol can be accomplished by constructing a microbial organism that contains biosynthetic pathways for conversion 25 of one pathway intermediate to another pathway intermediate or the product. Alternatively, 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3 buten- 1 -ol also can be biosynthetically produced from microbial organisms through co culture or co-fermentation using two organisms in the same vessel, where the first microbial organism produces a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, 30 crotyl alcohol or 3-buten-l-ol intermediate and the second microbial organism converts the intermediate to 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol.

48 Given the teachings and guidance provided herein, those skilled in the art will understand that a wide variety of combinations and permutations exist for the non-naturally occurring microbial organisms and methods of the invention together with other microbial organisms, with the co-culture of other non-naturally occurring microbial organisms 5 having subpathways and with combinations of other chemical and/or biochemical procedures well known in the art to produce 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten- 1 -ol. Sources of encoding nucleic acids for a 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten-l-ol pathway enzyme or protein can include, for 10 example, any species where the encoded gene product is capable of catalyzing the referenced reaction. Such species include both prokaryotic and eukaryotic organisms including, but not limited to, bacteria, including archaea and eubacteria, and eukaryotes, including yeast, plant, insect, animal, and mammal, including human. Exemplary species for such sources include, for example, Escherichia coli, Acetobacter aceti, Acetobacter 15 pasteurians, Achromobacter denitrificans, Acidaminococcusfermentans, Acinetobacter baumanii, Acinetobacter baylyi, Acinetobacter calcoaceticus, Acinetobacter sp. ADPI, Acinetobacter sp. Strain M-1, Actinobacillus succinogenes, Acyrthosiphon pisum, Aeropyrum pernix, Agrobacterium tumefaciens, Allochromatium vinosum DSM 180, Anabaena variabilis, Anaerobiospirillum succiniciproducens, Anaerostipes caccae DSM 20 14662, Anaerotruncus colihominis, Antheraea yamamai, Aquifex aeolicus, Arabidopsis thaliana, Archaeglubusfulgidus, Archaeoglobusfulgidus, Aromatoleum aromaticum EbNJ, Ascaris suum, Ascarius suum, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Aspergillus terreus, Aspergillus terreus NIH2624, Azoarcus sp. CIB, Azoarcus sp. T, Azotobacter vinelandii DJ, Anabaena variabilis, Bacillus anthracis, Bacillus 25 amyloliquefaciens, Bacillus cereus, Bacillus coahuilensis, Bacillus megaterium, Bacillus pseudofirmus, Bacillus pumilus, Bacillus sphaericus, Bacillus subtilis ,Bacteroides capillosus, Balnearium lithotrophicum, Bos taurus, Bradyrhizobiumjaponicum, Bradyrhizobium japonicum USDA 110, Brass ica napsus, Burkholderia ambifaria AMMD, Burkholderia phymatum, Burkholderia xenovorans, butyrate-producing bacterium L2-50, 30 butyrate-producing bacterium L2-50, butyrate-producing bacterium SS3/4, Campylobacter curvus 525.92, Campylobacterjejuni, Candida albicans, Candida parapsilosis ,Candida tropicalis, Carboxydothermus hydrogenoformans, Chlamydomonas reinhardtii, Chlorobium limicola, Chlorobium phaeobacteroides DSM 266, Chlorobium tepidum, Chlorobium tepidum, Chloroflexus aurantiacus, Citrobacter amalonaticus, 49 Citrobacter youngae A TCC 29220, Clostridium acetobutylicum, Clostridium aminobutyricum, Clostridium beierinckii, Clostridium beijerinckii NRRL B593, Clostridium botulinum, Clostridium botulinum A 3 str, Clostridium botulinum C str. Eklund, Clostridium carboxidivorans P7, Clostridium carboxidivorans P7, Clostridium 5 cellulolyticum HO, Clostridium k/uyveri, Clostridium k/uyveri DSM 555, Clostridium novyi NT, Clostridium pasteurianum, Clostridium propionicum, Clostridium saccharoperbutylacetonicum, Clostridium sp. SS2/1, Clostridium tetani, Clostridium tetanomorphum,Clostridium tyrobutyricum, Comamonas sp. CNB-1, Corynebacterium glutamicum, Corynebacterium glutamicum A TCC 13032, Corynebacterium glutanicum, 10 Cucumis sativus, Cupriavidus necator, Cupriavidus taiwanensis, Cyanobium PCC7001, Desulfovibrio africanus, DesulfoVibrio desulfuricans G20, Desulfovibrio desulfuricans subsp. desulfuricans str. A TCC 27774, Desulfovibrio fructosovorans JJ, Desulfovibrio vulgaris str. Hildenborough, Dictyostelium discoideum AX4, Drosophila melanogaster, Elizabethkingia meningoseptica, Erythrobacter sp. NAP], Escherichia coli C, Escherichia 15 coli K12, Escherichia coli K12 subsp. MG1655, Escherichia coli 0157:H7 str. Sakai, Escherichia coli str. K-12 substr. MG1655, Escherichia coli W, Eubacterium barkeri, Eubacterium rectale A TCC 33656, Eubacterium yurii, Euglena gracilis, Flavobacterium lutescens, Fusobacterium gonidiaformans, Fusobacterium nucleatum, Geobacillus stearothermophilus, Geobacillus thermoglucosidasius , Geobacter metallireducens GS-1 5, 20 Geobacter sulfurreducens, Gibberella zeae, Haemophilus influenza, Haloarcula marismortui, Halobacillus dabanensis, Halobacterium salinarum, Haloferax mediterranei, Helicobacter pylori, Helicobacterpylori 26695, Helicoverpa zea, Heliobacterpylori, Homo sapiens, Hydrogenobacter thermophilus, Jeotgalicoccus sp. A TCC8456, Klebsiella oxytoca, Klebsiella pneumonia, Klebsiella pneumonia, 25 Kluyveromyces lactis, Kocuria rosea, Lactobacillusplantarum, Lactobacillus sp. 30a, Lactococcus lactis, Leuconostoc mesenteroides, Macrococcus caseolyticus, Mannheimia succiniciproducens, marine gamma proteobacterium HTCC2080, Marinococcus halophilus, Marinomonas mediterranea, Medicago truncatula, Mesorhizobium loti, Metallosphaera sedula, Methanocaldococcusjannaschii, Methanosarcina thermophila, 30 Methanothermobacter thermautotrophicus, Methylobacterium extorquens, Moorella thermoacetica, Mus musculus, Musca domestica, Mycobacterium avium, Mycobacterium avium subsp. paratuberculosis K-10, Mycobacterium avium subsp. Pratuberculosis, Mycobacterium bovis BCG, Mycobacterium marinum M, Mycobacterium smegmatis, Mycobacterium smegmatis MC2 155, Mycobacterium tuberculosis, Natranaerobius 50 thermophilus, Neosartoryafischeri, Nicotiana glutinosa, Nicotiana tabacum, Nocardia farcinica IFM 10152, Nocardia iowensis, Nostoc sp. PCC 7120, Oryctolagus cuniculus, Oryza sativa, Paracoccus denitrificans, Pedicoccus pentosaceus, Pelobacter carbinolicus DSM 2380, Pelotomaculum thermopropionicum, Penicillium chrysogenum, Peptoniphilus 5 harei, Pichia stipitis, Porphyromonas gingivalis, Pseudoalteromonas tunicate, Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO], Pseudomonasfluorescens, Pseudomonasfluorescens KU- 7, Pseudomonasfluorescens Pf-5, Pseudomonas knackmussii (B13), Pseudomonas mendocina, Pseudomonas putida, Pseudomonasputida KT2440, Pseudomonas reinekei MTJ, Pseudomonas sp, Pseudomonas sp. CF600, 10 Pseudomonas sp. CF600, Pseudomonas sp. CF600, Pseudomonas sp. strain B13, Pseudomonas stutzeri, Pseudoramibacter alactolyticus, Psychroflexus torquis A TCC 700755, Pyrobaculum aerophilum str. IM2, Pyrococcusfuriosus, Ralstonia eutropha, Ralstonia eutropha H16, Ralstonia eutropha JMP134, Ralstonia metallidurans, Ralstonia pickettii, Rattus norvegicus, Rhizobium leguminosarum, Rhodobacter capsulates, 15 Rhodobacter capsulatus, Rhodobacter sphaeroides, Rhodococcus opacus, Rhodococcus ruber, Rhodopseudomonas palustris, Rhodopseudomonas palustris CGA 009, Rhodospirillum rubrum, Roseburia intestinalis LJ-82, Roseburia inulinivorans, Roseburia sp. A 2-183, Roseiflexus castenholzii, Saccharomyces cerevisae ,Salinispora arenicola, Salmonella enteric, Salmonella enterica subsp. arizonae serovar, Salmonella 20 typhimurium, Salmonella typhimurium LT2, Schizosaccharomyces pombe, Selenomonas ruminantium, Serratia marcescens, Simmondsia chinensis, Solibacillus silvestris, Sordaria macrospora, Sporosarcina newyorkensis, Staphylococcus pseudintermedius, Streptococcus mutans, Streptococcus oligofermentans, Streptococcus pyogenes A TCC 10782, Streptomyces clavuligenus, Streptomyces coelicolor, Streptomyces griseus, 25 Streptomyces griseus subsp. griseus ABRC 13350, Sulfolobus acidocalarius, Sulfolobus sp. strain 7, Sulfolobus tokodaii, Sulfolobus tokodaii 7, Sulfurihydrogenibium subterraneum, Sulfurimonas denitrificans, Sus scrofa, Synechocystis str. PCC 6803, Syntrophus aciditrophicus, Thauera aromatic, Thauera aromatic, Thermoanaerobacter brockii HTD4, Thermocrinis albus, Thermoproteus neutrophilus, Thermotoga maritime, 30 Thermus thermophilus, Thiobacillus denitrificans, Treponema denticola, Trichomonas vaginalis G3 ,Trypanosoma brucei, Tsukamurella paurometabola DSM 20162, Vibrio cholera, Vibrio parahaemolyticus , Vibrio vulnificus, Vitis vinfera, Yarrowia lipolytica, Yersinia intermedia A TCC 29909, Zea mays, Zoogloea ramigera, Zymomonas mobilis, Carthamus tinctorius, Cuphea hookeriana, Cuphea palustris, Cyanothece sp. PCC 7425, 51 Elizabethkingia meningoseptica, Lyngbya sp. PCC 8106, Nodularia spumigena CCY9414, Nostoc azollae, Plasmodiumfalciparum, Prochlarococcus marinus, Streptococcus pneumoniae, Streptococcus pyogenes A TCC 10782, Streptomyces avermitillis, Synechococcus elongatus, Synechacoccus elongatus PCC7942, Thermomyces 5 lanuginosus, Umbellularia caifornica, Arabidopsis thaliana col, Enterococcusfaecalis, Mycoplasmapneumoniae M129, Populus alba, Populus tremula, Pueraria montana, Staphylococcus aureus, Streptomyces sp. ACT-I, Thermotoga maritime MSB8, Streptomyces sp CL190, Streptomyces sp. KO-3988, Streptomyces cinnamonensis, Streptomyces anulatus, Nocardia brasiliensis as well as other exemplary species disclosed 10 herein are available as source organisms for corresponding genes. However, with the complete genome sequence available for now more than 550 species (with more than half of these available on public databases such as the NCBI), including 395 microorganism genomes and a variety of yeast, fungi, plant, and mammalian genomes, the identification of genes encoding the requisite 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, 15 crotyl alcohol or 3-buten- 1 -ol biosynthetic activity for one or more genes in related or distant species, including for example, homologues, orthologs, paralogs and nonorthologous gene displacements of known genes, and the interchange of genetic alterations between organisms is routine and well known in the art. Accordingly, the metabolic alterations allowing biosynthesis of 2,4-pentadienoate, butadiene, propylene, 20 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol described herein with reference to a particular organism such as E. coli can be readily applied to other microorganisms, including prokaryotic and eukaryotic organisms alike. Given the teachings and guidance provided herein, those skilled in the art will know that a metabolic alteration exemplified in one organism can be applied equally to other organisms. 25 In some instances, such as when an alternative 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol biosynthetic pathway exists in an unrelated species, 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3 buten-1-ol biosynthesis can be conferred onto the host species by, for example, exogenous expression of a paralog or paralogs from the unrelated species that catalyzes a similar, yet 30 non-identical metabolic reaction to replace the referenced reaction. Because certain differences among metabolic networks exist between different organisms, those skilled in the art will understand that the actual gene usage between different organisms may differ. However, given the teachings and guidance provided herein, those skilled in the art also will understand that the teachings and methods of the invention can be applied to all 52 microbial organisms using the cognate metabolic alterations to those exemplified herein to construct a microbial organism in a species of interest that will synthesize 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. Methods for constructing and testing the expression levels of a non-naturally occurring 5 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol producing host can be performed, for example, by recombinant and detection methods well known in the art. Such methods can be found described in, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, Third Ed., Cold Spring Harbor Laboratory, New York (2001); and Ausubel et al., Current Protocols in Molecular Biology, John 10 Wiley and Sons, Baltimore, MD (1999). Exogenous nucleic acid sequences involved in a pathway for production of 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol can be introduced stably or transiently into a host cell using techniques well known in the art including, but not limited to, conjugation, electroporation, chemical transformation, 15 transduction, transfection, and ultrasound transformation. For exogenous expression in E. coli or other prokaryotic cells, some nucleic acid sequences in the genes or cDNAs of eukaryotic nucleic acids can encode targeting signals such as an N-terminal mitochondrial or other targeting signal, which can be removed before transformation into prokaryotic host cells, if desired. For example, removal of a mitochondrial leader sequence led to 20 increased expression in E. coli (Hoffineister et al., J. Biol. Chem. 280:4329-4338 (2005)). For exogenous expression in yeast or other eukaryotic cells, genes can be expressed in the cytosol without the addition of leader sequence, or can be targeted to mitochondrion or other organelles, or targeted for secretion, by the addition of a suitable targeting sequence such as a mitochondrial targeting or secretion signal suitable for the host cells. Thus, it is 25 understood that appropriate modifications to a nucleic acid sequence to remove or include a targeting sequence can be incorporated into an exogenous nucleic acid sequence to impart desirable properties. Furthermore, genes can be subjected to codon optimization with techniques well known in the art to achieve optimized expression of the proteins. An expression vector or vectors can be constructed to include one or more 2,4 30 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1 -ol biosynthetic pathway encoding nucleic acids as exemplified herein operably linked to expression control sequences functional in the host organism. Expression vectors applicable for use in the microbial host organisms of the invention include, for example, 53 plasmids, phage vectors, viral vectors, episomes and artificial chromosomes, including vectors and selection sequences or markers operable for stable integration into a host chromosome. Additionally, the expression vectors can include one or more selectable marker genes and appropriate expression control sequences. Selectable marker genes also 5 can be included that, for example, provide resistance to antibiotics or toxins, complement auxotrophic deficiencies, or supply critical nutrients not in the culture media. Expression control sequences can include constitutive and inducible promoters, transcription enhancers, transcription terminators, and the like which are well known in the art. When two or more exogenous encoding nucleic acids are to be co-expressed, both nucleic acids 10 can be inserted, for example, into a single expression vector or in separate expression vectors. For single vector expression, the encoding nucleic acids can be operationally linked to one common expression control sequence or linked to different expression control sequences, such as one inducible promoter and one constitutive promoter. The transformation of exogenous nucleic acid sequences involved in a metabolic or synthetic 15 pathway can be confirmed using methods well known in the art. Such methods include, for example, nucleic acid analysis such as Northern blots or polymerase chain reaction (PCR) amplification of mRNA, or immunoblotting for expression of gene products, or other suitable analytical methods to test the expression of an introduced nucleic acid sequence or its corresponding gene product. It is understood by those.skilled in the art that 20 the exogenous nucleic acid is expressed in a sufficient amount to produce the desired product, and it is further understood that expression levels can be optimized to obtain sufficient expression using methods well known in the art and as disclosed herein. Suitable purification and/or assays to test for the production of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol can be performed 25 using well known methods. Suitable replicates such as triplicate cultures can be grown for each engineered strain to be tested. For example, product and byproduct formation in the engineered production host can be monitored. The final product and intermediates, and other organic compounds, can be analyzed by methods such as HPLC (High Performance Liquid Chromatography), GC-MS (Gas Chromatography-Mass Spectroscopy) and LC-MS 30 (Liquid Chromatography-Mass Spectroscopy) or other suitable analytical methods using routine procedures well known in the art. The release of product in the fermentation broth can also be tested with the culture supernatant. Byproducts and residual glucose can be quantified by HPLC using, for example, a refractive index detector for glucose and alcohols, and a UV detector for organic acids (Lin et al., Biotechnol. Bioeng. 90:775-779 54 (2005)), or other suitable assay and detection methods well known in the art. The individual enzyme or protein activities from the exogenous DNA sequences can also be assayed using methods well known in the art. The 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1 5 ol can be separated from other components in the culture using a variety of methods well known in the art. Such separation methods include, for example, extraction procedures as well as methods that include continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, size 10 exclusion chromatography, adsorption chromatography, and ultrafiltration. All of the above methods are well known in the art. Any of the non-naturally occurring microbial organisms described herein can be cultured to produce and/or secrete the biosynthetic products of the invention. For example, the 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol 15 producers can be cultured for the biosynthetic production of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. For the production of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol, the recombinant strains are cultured in a medium with carbon source and other essential nutrients. It is sometimes desirable and can be highly desirable 20 to maintain anaerobic conditions in the fermenter to reduce the cost of the overall process. Such conditions can be obtained, for example, by first sparging the medium with nitrogen and then sealing the flasks with a septum and crimp-cap. For strains where growth is not observed anaerobically, microaerobic or substantially anaerobic conditions can be applied by perforating the septum with a small hole for limited aeration. Exemplary anaerobic 25 conditions have been described previously and are well-known in the art. Exemplary aerobic and anaerobic conditions are described, for example, in United State publication 2009/0047719, filed August 10, 2007. Fermentations can be performed in a batch, fed batch or continuous manner, as disclosed herein. Fermentations can also be conducted in two phases, if desired. The first phase can be aerobic to allow for high growth and 30 therefore high productivity, followed by an anaerobic phase of high 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol yields.

55 If desired, the pH of the medium can be maintained at a desired pH, in particular neutral pH, such as a pH of around 7 by addition of a base, such as NaOH or other bases, or acid, as needed to maintain the culture medium at a desirable pH. The growth rate can be determined by measuring optical density using a spectrophotometer (600 urn), and the 5 glucose uptake rate by monitoring carbon source depletion over time. The growth medium can include, for example, any carbohydrate source which can supply a source of carbon to the non-naturally occurring microorganism. Such sources include, for example, sugars such as glucose, xylose, arabinose, galactose, mannose, fructose, sucrose and starch. Other sources of carbohydrate include, for example, renewable 10 feedstocks and biomass. Exemplary types of biomasses that can be used as feedstocks in the methods of the invention include cellulosic biomass, hemicellulosic biomass and lignin feedstocks or portions of feedstocks. Such biomass feedstocks contain, for example, carbohydrate substrates useful as carbon sources such as glucose, xylose, arabinose, galactose, mannose, fructose and starch. Given the teachings and guidance provided 15 herein, those skilled in the art will understand that renewable feedstocks and biomass other than those exemplified above also can be used for culturing the microbial organisms of the invention for the production of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. In addition to renewable feedstocks such as those exemplified above, the 2,4 20 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol microbial organisms of the invention also can be modified for growth on syngas as its source of carbon. In this specific embodiment, one or more proteins or enzymes are expressed in the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol producing organisms to provide a metabolic pathway for utilization of syngas 25 or other gaseous carbon source. Synthesis gas, also known as syngas or producer gas, is the major product of gasification of coal and of carbonaceous materials such as biomass materials, including agricultural crops and residues. Syngas is a mixture primarily of H 2 and CO and can be obtained from the gasification of any organic feedstock, including but not limited to coal, coal oil, natural 30 gas, biomass, and waste organic matter. Gasification is generally carried out under a high fuel to oxygen ratio. Although largely H 2 and CO, syngas can also include CO 2 and other gases in smaller quantities. Thus, synthesis gas provides a cost effective source of gaseous carbon such as CO and, additionally, CO 2

.

56 The Wood-Ljungdahl pathway catalyzes the conversion of CO and H 2 to acetyl-CoA and other products such as acetate. Organisms capable of utilizing CO and syngas also generally have the capability of utilizing CO 2 and CO 2

/H

2 mixtures through the same basic set of enzymes and transformations encompassed by the Wood-Ljungdahl pathway. H 2 5 dependent conversion of CO 2 to acetate by microorganisms was recognized long before it was revealed that CO also could be used by the same organisms and that the same pathways were involved. Many acetogens have been shown to grow in the presence of

CO

2 and produce compounds such as acetate as long as hydrogen is present to supply the necessary reducing equivalents (see for example, Drake, Acetogenesis, pp. 3-60 Chapman 10 and Hall, New York, (1994)). This can be summarized by the following equation: 2 CO 2 + 4 H 2 + n ADP + n Pi -+ CH 3 COOH + 2 H 2 0 + n ATP Hence, non-naturally occurring microorganisms possessing the Wood-Ljungdahl pathway can utilize CO 2 and H 2 mixtures as well for the production of acetyl-CoA and other desired products. 15 The Wood-Ljungdahl pathway is well known in the art and consists of 12 reactions which can be separated into two branches: (1) methyl branch and (2) carbonyl branch. The methyl branch converts syngas to methyl-tetrahydrofolate (methyl-THF) whereas the carbonyl branch converts methyl-THF to acetyl-CoA. The reactions in the methyl branch are catalyzed in order by the following enzymes or proteins: ferredoxin oxidoreductase, 20 formate dehydrogenase, formyltetrahydrofolate synthetase, methenyltetrahydrofolate cyclodehydratase, methylenetetrahydrofolate dehydrogenase and methylenetetrahydrofolate reductase. The reactions in the carbonyl branch are catalyzed in order by the following enzymes or proteins: methyltetrahydrofolate:corrinoid protein methyltransferase (for example, AcsE), corrinoid iron-sulfur protein, nickel-protein 25 assembly protein (for example, AcsF), ferredoxin, acetyl-CoA synthase, carbon monoxide dehydrogenase and nickel-protein assembly protein (for example, CooC). Following the teachings and guidance provided herein for introducing a sufficient number of encoding nucleic acids to generate a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway, those skilled in the art will understand that the same 30 engineering design also can be performed with respect to introducing at least the nucleic acids encoding the Wood-Ljungdahl enzymes or proteins absent in the host organism. Therefore, introduction of one or more encoding nucleic acids into the microbial 57 organisms of the invention such that the modified organism contains the complete Wood Ljungdahl pathway will confer syngas utilization ability. Additionally, the reductive (reverse) tricarboxylic acid cycle coupled with carbon monoxide dehydrogenase and/or hydrogenase activities can also be used for the 5 conversion of CO, CO 2 and/or H 2 -to acetyl-CoA and other products such as acetate. Organisms capable of fixing carbon via the reductive TCA pathway can utilize one or more of the following enzymes: ATP citrate-lyase, citrate lyase, aconitase, isocitrate dehydrogenase, alpha-ketoglutarate:ferredoxin oxidoreductase, succinyl-CoA synthetase, succinyl-CoA transferase, fumarate reductase, fumarase, malate dehydrogenase, 10 NAD(P)H:ferredoxin oxidoreductase, carbon monoxide dehydrogenase, and hydrogenase. Specifically, the reducing equivalents extracted from CO and/or H 2 by carbon monoxide dehydrogenase and hydrogenase are utilized to fix CO 2 via the reductive TCA cycle into acetyl-CoA or acetate. Acetate can be converted to acetyl-CoA by enzymes such as acetyl-CoA transferase, acetate kinase/phosphotransacetylase, and acetyl-CoA synthetase. 15 Acetyl-CoA can be converted to the 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten-1-ol precursors, glyceraldehyde-3-phosphate, phosphoenolpyruvate, and pyruvate, by pyruvate:ferredoxin oxidoreductase and the enzymes of gluconeogenesis. Following the teachings and guidance provided herein for introducing a sufficient number of encoding nucleic acids to generate a 2,4-pentadienoate, 20 butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol pathway, those skilled in the art will understand that the same engineering design also can be performed with respect to introducing at least the nucleic acids encoding the reductive TCA pathway enzymes or proteins absent in the host organism. Therefore, introduction of one or more encoding nucleic acids into the microbial organisms of the invention such that the 25 modified organism contains a reductive TCA pathway can confer syngas utilization ability. Thus, this invention is also directed, in part to engineered biosynthetic pathways to improve carbon flux through a central metabolism intermediate en route to 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-I -ol. The 30 present invention provides non-naturally occurring microbial organisms having one or more exogenous genes encoding enzymes that can catalyze various enzymatic transformations en route to 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. In some embodiments, these enzymatic transformations are part 58 of the reductive tricarboxylic acid (RTCA) cycle and are used to improve product yields, including but not limited to, from carbohydrate-based carbon feedstock. In numerous engineered pathways, realization of maximum product yields based on carbohydrate feedstock is hampered by insufficient reducing equivalents or by loss of 5 reducing equivalents and/or carbon to byproducts. In accordance with some embodiments, the present invention increases the yields of 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten-1-ol by (i) enhancing carbon fixation via the reductive TCA cycle, and/or (ii) accessing additional reducing equivalents from gaseous carbon sources and/or syngas components such as CO, C0 2 , and/or H 2 . In addition to 10 syngas, other sources of such gases include, but are not listed to, the atmosphere, either as found in nature or generated. The C0 2 -fixing reductive tricarboxylic acid (RTCA) cycle is an endergenic anabolic pathway of CO 2 assimilation which uses reducing equivalents and ATP (Figure 8). One turn of the RTCA cycle assimilates two moles of CO 2 into one mole of acetyl-CoA, or 15 four moles of CO 2 into one mole of oxaloacetate. This additional availability of acetyl CoA improves the maximum theoretical yield of product molecules derived from carbohydrate-based carbon feedstock. Exemplary carbohydrates include but are not limited to glucose, sucrose, xylose, arabinose and glycerol. In some embodiments, the reductive TCA cycle, coupled with carbon monoxide 20 dehydrogenase and/or hydrogenase enzymes, can be employed to allow syngas, C0 2 , CO,

H

2 , and/or other gaseous carbon source utilization by microorganisms. Synthesis gas (syngas), in particular is a mixture of primarily H 2 and CO, sometimes including some amounts of CO 2 , that can be obtained via gasification of any organic feedstock, such as coal, coal oil, natural gas, biomass, or waste organic matter. Numerous gasification 25 processes have been developed, and most designs are based on partial oxidation, where limiting oxygen avoids full combustion, of organic materials at high temperatures (500 1500"C) to provide syngas as a 0.5:1-3:1 H 2 /CO mixture. In addition to coal, biomass of many types has been used for syngas production and represents an inexpensive and flexible feedstock for the biological production of renewable chemicals and fuels. Carbon 30 dioxide can be provided from the atmosphere or in condensed from, for example, from a tank cylinder, or via sublimation of solid CO 2 . Similarly, CO and hydrogen gas can be provided in reagent form and/or mixed in any desired ratio. Other gaseous carbon forms can include, for example, methanol or similar volatile organic solvents.

59 The components of synthesis gas and/or other carbon sources can provide sufficient C0 2 , reducing equivalents, and ATP for the reductive TCA cycle to operate. One turn of the RTCA cycle assimilates two moles of CO 2 into one mole of acetyl-CoA and requires 2 ATP and 4 reducing equivalents. CO and/or H 2 can provide reducing equivalents by 5 means of carbon monoxide dehydrogenase and hydrogenase enzymes, respectively. Reducing equivalents can come in the form of NADH, NADPH, FADH, reduced quinones, reduced ferredoxins, reduced flavodoxins and thioredoxins. The reducing equivalents, particularly NADH, NADPH, and reduced ferredoxin, can serve as cofactors for the RTCA cycle enzymes, for example, malate dehydrogenase, fumarate reductase, 10 alpha-ketoglutarate:ferredoxin oxidoreductase (alternatively known as 2 oxoglutarate:ferredoxin oxidoreductase, alpha-ketoglutarate synthase, or 2-oxoglutarate synthase), pyruvate:ferredoxin oxidoreductase and isocitrate dehydrogenase. The electrons from these reducing equivalents can alternatively pass through an ion-gradient producing electron transport chain where they are passed to an acceptor such as oxygen, 15 nitrate, oxidized metal ions, protons, or an electrode. The ion-gradient can then be used for ATP generation via an ATP synthase or similar enzyme. The reductive TCA cycle was first reported in the green sulfur photosynthetic bacterium Chlorobium limicola (Evans et aL, Proc. Natl. Acad. Sci. U.S.A. 55:928-934 (1966)). Similar pathways have been characterized in some prokaryotes (proteobacteria, green 20 sulfur bacteria and thermophillic Knallgas bacteria) and sulfur-dependent archaea (Hugler et al., J. Bacteriol. 187:3020-3027 (2005; Hugler et al., Environ. Microbiol. 9:81-92 (2007). In some cases, reductive and oxidative (Krebs) TCA cycles are present in the same organism (Hugler et al., supra (2007); Siebers et al., J. Bacteriol. 186:2179-2194 (2004)). Some methanogens and obligate anaerobes possess incomplete oxidative or reductive 25 TCA cycles that may function to synthesize biosynthetic intermediates (Ekiel et al., J. Bacteriol. 162:905-908 (1985); Wood et al., FEMSMicrobiol. Rev. 28:335-352 (2004)). The key carbon-fixing enzymes of the reductive TCA cycle are alpha ketoglutarate:ferredoxin oxidoreductase, pyruvate:ferredoxin oxidoreductase and isocitrate dehydrogenase. Additional carbon may be fixed during the conversion of 30 phosphoenolpyruvate to oxaloacetate by phosphoenolpyruvate carboxylase or phosphoenolpyruvate carboxykinase or by conversion of pyruvate to malate by malic enzyme.

60 Many of the enzymes in the TCA cycle are reversible and can catalyze reactions in the reductive and oxidative directions. However, some TCA cycle reactions are irreversible in vivo and thus different enzymes are used to catalyze these reactions in the directions required for the reverse TCA cycle. These reactions are: (1) conversion of citrate to 5 oxaloacetate and acetyl-CoA, (2) conversion of fumarate to succinate, and (3) conversion of succinyl-CoA to alpha-ketoglutarate. In the TCA cycle, citrate is formed from the condensation of oxaloacetate and acetyl-CoA. The reverse reaction, cleavage of citrate to oxaloacetate and acetyl-CoA, is ATP-dependent and catalyzed by ATP-citrate lyase, or citryl-CoA synthetase and citryl-CoA lyase. Alternatively, citrate lyase can be coupled to 10 acetyl-CoA synthetase, an acetyl-CoA transferase, or phosphotransacetylase and acetate kinase to form acetyl-CoA and oxaloacetate from citrate. The conversion of succinate to fumarate is catalyzed by succinate dehydrogenase while the reverse reaction is catalyzed by fumarate reductase. In the TCA cycle succinyl-CoA is formed from the NAD(P)* dependent decarboxylation of alpha-ketoglutarate by the alpha-ketoglutarate 15 dehydrogenase complex. The reverse reaction is catalyzed by alpha ketoglutarate:ferredoxin oxidoreductase. An organism capable of utilizing the reverse tricarboxylic acid cycle to enable production of acetyl-CoA-derived products on 1) CO, 2) CO 2 and H2, 3) CO and C0 2 , 4) synthesis gas comprising CO and H 2 , and 5) synthesis gas or other gaseous carbon sources 20 comprising CO, C0 2 , and H 2 can include any of the following enzyme activities: ATP citrate lyase, citrate lyase, aconitase, isocitrate dehydrogenase, alpha ketoglutarate:ferredoxin oxidoreductase, succinyl-CoA synthetase, succinyl-CoA transferase, funarate reductase, fumarase, malate dehydrogenase, acetate kinase, phosphotransacetylase, acetyl-CoA synthetase, acetyl-CoA transferase, 25 pyruvate:ferredoxin oxidoreductase, NAD(P)H:ferredoxin oxidoreductase, carbon monoxide dehydrogenase, hydrogenase, and ferredoxin (see Figure 9). Enzymes and the corresponding genes required for these activities are described herein above. Carbon from syngas or other gaseous carbon sources can be fixed via the reverse TCA cycle and components thereof. Specifically, the combination of certain carbon gas 30 utilization pathway components with the pathways for formation of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol from acetyl-CoA results in high yields of these products by providing an efficient mechanism for fixing the 61 carbon present in carbon dioxide, fed exogenously or produced endogenously from CO, into acetyl-CoA. In some embodiments, a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway in a non-naturally occurring microbial organism of the 5 invention can utilize any combination of (1) CO, (2) C0 2 , (3) H 2 , or mixtures thereof to enhance the yields of biosynthetic steps involving reduction, including addition to driving the reductive TCA cycle. In some embodiments a non-naturally occurring microbial organism having an 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1 -ol 10 pathway includes at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme. The at least one exogenous nucleic acid is selected from an ATP-citrate lyase, citrate lyase, a fumarate reductase, isocitrate dehydrogenase, aconitase, and an alpha ketoglutarate:ferredoxin oxidoreductase; and at least one exogenous enzyme selected from a carbon monoxide dehydrogenase, a hydrogenase, a NAD(P)H:ferredoxin 15 oxidoreductase, and a ferredoxin, expressed in a sufficient amount to allow the utilization of (1) CO, (2) CO 2 , (3) H 2 , (4) CO 2 and H 2 , (5) CO and C0 2 , (6) CO and H 2 , or (7) CO, C0 2 , and H 2 . In some embodiments a method includes culturing a non-naturally occurring microbial organism having a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol 20 or 3-buten-1-ol pathway also comprising at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme. The at least one exogenous nucleic acid is selected from an ATP-citrate lyase, citrate lyase, a fumarate reductase, isocitrate dehydrogenase, aconitase, and an alpha-ketoglutarate:ferredoxin oxidoreductase. Additionally, such an organism can also include at least one exogenous enzyme selected from a carbon 25 monoxide dehydrogenase, a hydrogenase, a NAD(P)H:ferredoxin oxidoreductase, and a ferredoxin, expressed in a sufficient amount to allow the utilization of (1) CO, (2) C0 2 , (3)

H

2 , (4) CO 2 and H 2 , (5) CO and C0 2 , (6) CO and H 2 , or (7) CO, CO 2 , and H 2 to produce a product. In some embodiments a non-naturally occurring microbial organism having an 2,4 30 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol pathway further includes at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme expressed in a sufficient amount to enhance carbon flux through acetyl- 62 CoA. The at least one exogenous nucleic acid is selected from an ATP-citrate lyase, citrate lyase, a fumarate reductase, a pyruvate:ferredoxin oxidoreductase, isocitrate dehydrogenase, aconitase and an alpha-ketoglutarate:ferredoxin oxidoreductase. In some embodiments a non-naturally occurring microbial organism having an 2,4 5 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide and/or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. The at least one exogenous nucleic acid is 10 selected from a carbon monoxide dehydrogenase, a hydrogenase, an NAD(P)H:ferredoxin oxidoreductase, and a ferredoxin. In some embodiments, the present invention provides a method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock, such as sugars or gaseous carbon sources, the 15 method includes culturing this non-naturally occurring microbial organism under conditions and for a sufficient period of time to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. In some embodiments, the non-naturally occurring microbial organism having an 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol 20 pathway includes two exogenous nucleic acids, each encoding a reductive TCA pathway enzyme. In some embodiments, the non-naturally occurring microbial organism having an 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol pathway includes three exogenous nucleic acids each encoding a reductive TCA pathway enzyme. In some embodiments, the non-naturally occurring microbial organism includes 25 three.exogenous nucleic acids encoding an ATP-citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase. In some embodiments, the non-naturally occurring microbial organism includes three exogenous nucleic acids encoding a citrate lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase. In some embodiments, the non-naturally occurring microbial organism includes four 30 exogenous nucleic acids encoding a pyruvate:ferredoxin oxidoreductase; a phosphoenolpyruvate carboxylase or a phosphoenolpyruvate carboxykinase, a CO dehydrogenase; and an H 2 hydrogenase. In some embodiments, the non-naturally 63 occurring microbial organism includes two exogenous nucleic acids encoding a CO dehydrogenase and an H 2 hydrogenase. In some embodiments, the non-naturally occurring microbial organisms having an 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1 -ol 5 pathway further include an exogenous nucleic acid encoding an enzyme selected from a pyruvate:ferredoxin oxidoreductase, an aconitase, an isocitrate dehydrogenase, a succinyl CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, an acetate kinase, a phosphotransacetylase, an acetyl-CoA synthetase, an NAD(P)H:ferredoxin oxidoreductase, and combinations thereof. 10 In some embodiments, the non-naturally occurring microbial organism having an 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol pathway further includes an exogenous nucleic acid encoding an enzyme selected from carbon monoxide dehydrogenase, acetyl-CoA synthase, ferredoxin, NAD(P)H:ferredoxin oxidoreductase and combinations thereof. 15 In some embodiments, the non-naturally occurring microbial organism having an 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol pathway utilizes a carbon feedstock selected from (1) CO, (2) C0 2 , (3) CO 2 and H 2 , (4) CO and H 2 , or (5) CO, C0 2 , and H 2 . In some embodiments, the non-naturally occurring microbial organism having an 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, 20 crotyl alcohol or 3-buten-1-ol pathway utilizes hydrogen for reducing equivalents. In some embodiments, the non-naturally occurring microbial organism having an 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol pathway utilizes CO for reducing equivalents. In some embodiments, the non-naturally occurring microbial organism having an 2,4-pentadienoate, butadiene, propylene, 1,3 25 butanediol, crotyl alcohol or 3-buten-l-ol pathway utilizes combinations of CO and hydrogen for reducing equivalents. In some embodiments, the non-naturally occurring microbial organism having an 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway further includes one or more nucleic acids encoding an enzyme selected from a 30 phosphoenolpyruvate carboxylase, a phosphoenolpyruvate carboxykinase, a pyruvate carboxylase, and a malic enzyme.

64 In some embodiments, the non-naturally occurring microbial organism having an 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway further includes one or more nucleic acids encoding an enzyme selected from a malate dehydrogenase, a fumarase, a fumarate reductase, a succinyl-CoA synthetase, and a 5 succinyl-CoA transferase. In some embodiments, the non-naturally occurring microbial organism having an 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway further includes at least one exogenous nucleic acid encoding a citrate lyase, an ATP-citrate lyase, a citryl-CoA synthetase, a citryl-CoA lyase, an aconitase, an isocitrate 10 dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, an acetate kinase, a phosphotransacetylase, an acetyl-CoA synthetase, and a ferredoxin. Accordingly, given the teachings and guidance provided herein, those skilled in the art will understand that a non-naturally occurring microbial organism can be produced that 15 secretes the biosynthesized compounds of the invention when grown on a carbon source such as a carbohydrate. Such compounds include, for example, 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol and any of the intermediate metabolites in the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway. All that is required is to engineer in one or more 20 of the required enzyme or protein activities to achieve biosynthesis of the desired compound or intermediate including, for example, inclusion of some or all of the 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol biosynthetic pathways. Accordingly, the invention provides a non-naturally occurring microbial organism that produces and/or secretes 2,4-pentadienoate, butadiene, propylene, 25 1,3-butanediol, crotyl alcohol or 3-buten-1-ol when grown on a carbohydrate or other carbon source and produces and/or secretes any of the intermediate metabolites shown in the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway when grown on a carbohydrate or other carbon source. The 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol producing microbial 30 organisms of the invention can initiate synthesis from an intermediate, for example, 5 aminopent-2-enoate, glutarate semialdehyde, 5-hydroxyvalerate, 5-hydroxyvaleryl-CoA, 5-hydroxypent-2-enoyl-CoA, 2,4-pentadienoyl-CoA, 5-hydroxypent-2-enoate, 5 hydroxypent-2-enoate, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotoyl-CoA, glutaryl- 65 CoA, 3-oxopentanoyl-CoA, 3-hydroxypentanoyl-CoA, pent-2-enoyl-CoA, pent-3-enoyl CoA, 3-oxo-5-hydroxypentanoyl-CoA, 3-oxo-5-hydroxypentanoate, 3-oxobutanol, 4 hydroxy-2-oxovalerate, 2-oxopentenoate, 3-buten-1-al, 3-hydroxybutyryl-CoA, 3 hydroxybutyrate, 3-hydroxybutanal, 2,4-pentadienoyl-phosphate, or penta-2,4-dienal. 5 The non-naturally occurring microbial organisms of the invention are constructed using methods well known in the art as exemplified herein to exogenously express at least one nucleic acid encoding a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway enzyme or protein in sufficient amounts to produce 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol. It is 10 understood that the microbial organisms of the invention are cultured under conditions sufficient to produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. Following the teachings and guidance provided herein, the non naturally occurring microbial organisms of the invention can achieve biosynthesis of 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol 15 resulting in intracellular concentrations between about 0.1-200 mM or more. Generally, the intracellular concentration of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol is between about 3-150 mM, particularly between about 5 125 mM and more particularly between about 8-100 mM, including about 10 mM, 20 mM, 50 mM, 80 mM, or more. Intracellular concentrations between and above each of these 20 exemplary ranges also can be achieved from the non-naturally occurring microbial organisms of the invention. In some embodiments, culture conditions include anaerobic or substantially anaerobic growth or maintenance conditions. Exemplary anaerobic conditions have been described previously and are well known in the art, Exemplary anaerobic conditions for 25 fermentation processes are described herein and are described, for example, in U.S. publication 2009/0047719, filed August 10, 2007. Any of these conditions can be employed with the non-naturally occurring microbial organisms as well as other anaerobic conditions well known in the art. Under such anaerobic or substantially anaerobic conditions, the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 30 3-buten-1-ol producers can synthesize 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten-1-ol at intracellular concentrations of 5-10 mM or more as well as all other concentrations exemplified herein. It is understood that, even though the above description refers to intracellular concentrations, 2,4-pentadienoate, 66 butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol producing microbial organisms can produce 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol intracellularly and/or secrete the product into the culture medium. In addition to the culturing and fermentation conditions disclosed herein, growth condition 5 for achieving biosynthesis of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol can include the addition of an osmoprotectant to the culturing conditions. In certain embodiments, the non-naturally occurring microbial organisms of the invention can be sustained, cultured or fermented as described herein in the presence of an osmoprotectant. Briefly, an osmoprotectant refers to a compound that 10 acts as an osmolyte and helps a microbial organism as described herein survive osmotic stress. Osmoprotectants include, but are not limited to, betaines, amino acids, and the sugar trehalose. Non-limiting examples of such are glycine betaine, praline betaine, dimethylthetin, dimethylslfonioproprionate, 3-dimethylsulfonio-2-methylproprionate, pipecolic acid, dimethylsulfonioacetate, choline, L-carnitine and ectoine. In one aspect, 15 the osmoprotectant is glycine betaine. It is understood to one of ordinary skill in the art that the amount and type of osmoprotectant suitable for protecting a microbial organism described herein from osmotic stress will depend on the microbial organism used. The amount of osmoprotectant in the culturing conditions can be, for example, no more than about 0.1 mM, no more than about 0.5 mM, no more than about 1.0 mM, no more than 20 about 1.5 mM, no more than about 2.0 mM, no more than about 2.5 mM, no more than about 3.0 mM, no more than about 5.0 mM, no more than about 7.0 mM, no more than about 10mM, no more than about 50mM, no more than about 100mM or no more than about 500mM. In some embodiments, the carbon feedstock and other cellular uptake sources such as 25 phosphate, ammonia, sulfate, chloride and other halogens can be chosen to alter the isotopic distribution of the atoms present in 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten- 1 -ol or any 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway intermediate. The various carbon feedstock and other uptake sources enumerated above will be referred to herein, 30 collectively, as "uptake sources." Uptake sources can provide isotopic enrichment for any atom present in the product 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol or 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway intermediate, or for side products generated in reactions 67 diverging away from a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway. Isotopic enrichment can be achieved for any target atom including, for example, carbon, hydrogen, oxygen, nitrogen, sulfur, phosphorus, chloride or other halogens. 5 In some embodiments, the uptake sources can be selected to alter the carbon- 12, carbon 13, and carbon-14 ratios. In some embodiments, the uptake sources can be selected to alter the oxygen-16, oxygen-17, and oxygen-18 ratios. In some embodiments, the uptake sources can be selected to alter the hydrogen, deuterium, and tritium ratios. In some embodiments, the uptake sources can be selected to alter the nitrogen-14 and nitrogen-15 10 ratios. In some embodiments, the uptake sources can be selected to alter the sulfur-32, sulfur-33, sulfur-34, and sulfur-35 ratios. In some embodiments, the uptake sources can be selected to alter the phosphorus-3 1, phosphorus-32, and phosphorus-33 ratios. In some embodiments, the uptake sources can be selected to alter the chlorine-35, chlorine-36, and chlorine-37 ratios. 15 In some embodiments, the isotopic ratio of a target atom can be varied to a desired ratio by selecting one or more uptake sources. An uptake source can be derived from a natural source, as found in nature, or from a man-made source, and one skilled in the art can select a natural source, a man-made source, or a combination thereof, to achieve a desired isotopic ratio of a target atom. An example of a man-made uptake source includes, for 20 example, an uptake source that is at least partially derived from a chemical synthetic reaction. Such isotopically enriched uptake sources can be purchased commercially or prepared in the laboratory and/or optionally mixed with a natural source of the uptake source to achieve a desired isotopic ratio. In some embodiments, a target atom isotopic ratio of an uptake source can be achieved by selecting a desired origin of the uptake source 25 as found in nature. For example, as discussed herein, a natural source can be a biobased derived from or synthesized by a biological organism or a source such as petroleum-based products or the atmosphere. In some such embodiments, a source of carbon, for example, can be selected from a fossil fuel-derived carbon source, which can be relatively depleted of carbon-14, or an environmental or atmospheric carbon source, such as C0 2 , which can 30 possess a larger amount of carbon-14 than its petroleum-derived counterpart. The unstable carbon isotope carbon-14 or radiocarbon makes up for roughly 1 in 1012 carbon atoms in the earth's atmosphere and has a half-life of about 5700 years. The stock of carbon is replenished in the upper atmosphere by a nuclear reaction involving cosmic 68 rays and ordinary nitrogen (1 4 N). Fossil fuels contain no carbon-14, as it decayed long ago. Burning of fossil fuels lowers the atmospheric carbon-14 fraction, the so-called "Suess effect". Methods of determining the isotopic ratios of atoms in a compound are well known to 5 those skilled in the art. Isotopic enrichment is readily assessed by mass spectrometry using techniques known in the art such as accelerated mass spectrometry (AMS), Stable Isotope Ratio Mass Spectrometry (SIRMS) and Site-Specific Natural Isotopic Fractionation by Nuclear Magnetic Resonance (SNIF-NMR). Such mass spectral techniques can be integrated with separation techniques such as liquid chromatography 10 (LC), high performance liquid chromatography (HPLC) and/or gas chromatography, and the like. In the case of carbon, ASTM D6866 was developed in the United States as a standardized analytical method for determining the biobased content of solid, liquid, and gaseous samples using radiocarbon dating by the American Society for Testing and Materials 15 (ASTM) International. The standard is based on the use of radiocarbon dating for the determination of a product's biobased content. ASTM D6866 was first published in 2004, and the current active version of the standard is ASTM D6866- 11 (effective April 1, 2011). Radiocarbon dating techniques are well known to those skilled in the art, including those described herein. 20 The biobased content of a compound is estimated by the ratio of carbon-14 ( 14 C) to carbon-12 (1 2 C). Specifically, the Fraction Modem (Fm) is computed from the expression: Fm = (S-B)/(M-B), where B, S and M represent the 1 4 C/1 2 C ratios of the blank, the sample and the modern reference, respectively. Fraction Modem is a measurement of the deviation of the 4

C/

12 C ratio of a sample from "Modem." Modem is defined as 95% of the 25 radiocarbon concentration (in AD 1950) of National Bureau of Standards (NBS) Oxalic Acid I (i.e., standard reference materials (SRM) 4990b) normalized to 6 3 CVPDB=-19 per mil (Olsson, The use of Oxalic acid as a Standard. in, Radiocarbon Variations and Absolute Chronologv Nobel Symposium, 12th Proc., John Wiley & Sons, New York (1970)). Mass spectrometry results, for example, measured by ASM, are calculated using 30 the internationally agreed upon definition of 0.95 times the specific activity of NBS Oxalic Acid I (SRM 4990b) normalized to S 3 CvpDo=-1 9 per mil. This is equivalent to an absolute (AD 1950) 1 4 C/1 2 C ratio of 1.176 i 0.010 x 10-12 (Karlen et al., Arkiv Geofysik, 4:465-471 (1968)). The standard calculations take into account the differential uptake of 69 one istope with respect to another, for example, the preferential uptake in biological systems of C1 2 over C1 3 over C' 4 , and these corrections are reflected as a Fm corrected for 613. An oxalic acid standard (SRM 4990b or HOx 1) was made from a crop of 1955 sugar beet. 5 Although there were 1000 lbs made, this oxalic acid standard is no longer commercially available. The Oxalic Acid II standard (HOx 2; N.I.S.T designation SRM 4990 C) was made from a crop of 1977 French beet molasses. In the early 1980's, a group of 12 laboratories measured the ratios of the two standards. The ratio of the activity of Oxalic acid II to 1 is 1.2933±0.001 (the weighted mean), The isotopic ratio of HOx II is -17.8 per 10 mille. ASTM D6866-11 suggests use of the available Oxalic Acid II standard SRM 4990 C (Hox2) for the modem standard (see discussion of original vs. currently available oxalic acid standards in Mann, Radiocarbon, 25(2):519-527 (1983)). A Fm = 0% represents the entire lack of carbon-14 atoms in a material, thus indicating a fossil (for example, petroleum based) carbon source. A Fm = 100%, after correction for the post-1950 15 injection of carbon-14 into the atmosphere from nuclear bomb testing, indicates an entirely modem carbon source. As described herein, such a "modern" source includes biobased sources. As described in ASTM D6866, the percent modern carbon (pMC) can be greater than 100% because of the continuing but diminishing effects of the 1950s nuclear testing 20 programs, which resulted in a considerable enrichment of carbon-14 in the atmosphere as described in ASTM D6866- 11. Because all sample carbon-14 activities are referenced to a "pre-bomb" standard, and because nearly all new biobased products are produced in a post-bomb environment, all pMC values (after correction for isotopic fraction) must be multiplied by 0.95 (as of 2010) to better reflect the true biobased content of the sample, A 25 biobased content that is greater than 103% suggests that either an analytical error has occurred, or that the source of biobased carbon is more than several years old. ASTM D6866 quantifies the biobased content relative to the material's total organic content and does not consider the inorganic carbon and other non-carbon containing substances present. For example, a product that is 50% starch-based material and 50% 30 water would be considered to have a Biobased Content = 100% (50% organic content that is 100% biobased) based on ASTM D6866. In another example, a product that is 50% starch-based material, 25% petroleum-based, and 25% water would have a Biobased Content = 66.7% (75% organic content but only 50% of the product is biobased). In 70 another example, a product that is 50% organic carbon and is a petroleum-based product would be considered to have a Biobased Content = 0% (50% organic carbon but from fossil sources). Thus, based on the well known methods and known standards for determining the biobased content of a compound or material, one skilled in the art can 5 readily determine the biobased content and/or prepared downstream products that utilize of the invention having a desired biobased content. Applications of carbon-14 dating techniques to quantify bio-based content of materials are known in the art (Currie et al., Nuclear Instruments and Methods in Physics Research B, 172:281-287 (2000)). For example, carbon-14 dating has been used to quantify bio-based 10 content in terephthalate-containing materials (Colonna et aL, Green Chemistry, 13:2543 2548 (2011)). Notably, polypropylene terephthalate (PPT) polymers derived from renewable 1,3-propanediol and petroleum-derived terephthalic acid resulted in Fm values near 30% (i.e., since 3/11 of the polymeric carbon derives from renewable 1,3-propanediol and 8/11 from the fossil end member terephthalic acid) (Currie et aL, supra, 2000). In 15 contrast, polybutylene terephthalate polymer derived from both renewable 1,4-butanediol and renewable terephthalic acid resulted in bio-based content exceeding 90% (Colonna et al., supra, 2011). Accordingly, in some embodiments, the present invention provides 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol or a 2,4 20 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway intermediate that has a carbon- 12, carbon-13, and carbon- 14 ratio that reflects an atmospheric carbon, also referred to as environmental carbon, uptake source. For example, in some aspects the 2,4-pentadienoate, butadiene, propylene, I,3-butanediol, crotyl alcohol or 3-buten-l-ol or a 2,4-pentadienoate, butadiene, propylene, 1,3 25 butanediol, crotyl alcohol or 3-buten- 1 -ol pathway intermediate can have an Fm value of at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 98% or as much as 100%. In some such embodiments, the uptake source is CO 2 . In some embodiments, 30 the present invention provides 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol or a 2,4-pentadienoate, butadiene, propylene, 1,3 butanediol, crotyl alcohol or 3-buten-1-ol intermediate that has a carbon-12, carbon-13, and carbon-14 ratio that reflects petroleum-based carbon uptake source. In this aspect, the 71 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol or a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol pathway intermediate can have an Fm value of less than 95%, less than 90%, less than 85%, less than 80%, less than 75%, less than 70%, less than 65%, less than 60%, less than 5 55%, less than 50%, less than 45%, less than 40%, less than 35%, less than 30%, less than 25%, less than 20%, less than 15%, less than 10%, less than 5%, less than 2% or less than 1%. In some embodiments, the present invention provides 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol or a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol intermediate that has a 10 carbon-12, carbon-13, and carbon-14 ratio that is obtained by a combination of an atmospheric carbon uptake source with a petroleum-based uptake source. Using such a combination of uptake sources is one way by which the carbon-12, carbon-13, and carbon 14 ratio can be varied, and the respective ratios would reflect the proportions of the uptake sources. 15 Further, the present invention relates to the biologically produced 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol or 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1 -ol pathway intermediate as disclosed herein, and to the products derived therefrom, wherein the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol or a 2,4 20 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway intermediate has a carbon- 12, carbon- 13, and carbon- 14 isotope ratio of about the same value as the CO 2 that occurs in the environment. For example, in some aspects the invention provides: bioderived 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol or a bioderived 2,4-pentadienoate, butadiene, propylene, 25 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway intermediate having a carbon-12 versus carbon-13 versus carbon-14 isotope ratio of about the same value as the CO 2 that occurs in the environment, or any of the other ratios disclosed herein. It is understood, as disclosed herein, that a product can have a carbon-12 versus carbon-13 versus carbon-14 isotope ratio of about the same value as the CO 2 that occurs in the environment, or any of 30 the ratios disclosed herein, wherein the product is generated from bioderived 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol or a bioderived 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3 buten- 1 -ol intermediate as disclosed herein, wherein the bioderived product is chemically modified to generate a final product. Methods of chemically modifying a bioderived 72 product of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3 buten- 1 -ol, or an intermediate thereof, to generate a desired product are well known to those skilled in the art, as described herein. The invention further provides polyurethane, polymer, co-polymer, synthetic rubber, resin, chemical, polymer intermediate, organic 5 solvent, hypoglycaemic agent, polyester resin, latex, monomer, fine chemical, agricultural chemical, pharmaceutical, or perfume having a carbon-12 versus carbon-13 versus carbon 14 isotope ratio of about the same value as the CO 2 that occurs in the environment, wherein the polyurethane, polymer, co-polymer, synthetic rubber, resin, chemical, polymer intermediate, organic solvent, hypoglycaemic agent, polyester resin, latex, 10 monomer, fine chemical, agricultural chemical, pharmaceutical, or perfume is generated directly from or in combination with bioderived 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol or a bioderived 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol intermediate as disclosed herein. 15 2,4-Pentadienoate is a useful substituted butadiene derivative and a valuable intermediate en route to other substituted 1,3-butadiene derivatives, including, for example, 1 carbamoyl-1,3-butadienes. Non-limiting examples of applications of 2,4-pentadienoate include production of N-protected-1,3-butadiene derivatives that can be used in the preparation of anilines, a precursor to many inductrial chemicals, such as polyurethane and 20 production of various polymers and co-polymers. Accordingly, in some embodiments, the invention provides a biobased polyurethane, polymer or co-polymer comprising one or more bioderived 2,4-pentadienoate or bioderived 2,4-pentadienoate intermediate produced by a non-naturally occurring microorganism of the invention or produced using a method disclosed herein. 25 Butadiene is a chemical commonly used in many commercial and industrial applications. Non-limiting examples of such applications include production of polymers, such as synthetic rubbers and ABS resins, and chemicals, such as hexamethylenediamine and 1,4 butanediol . Accordingly, in some embodiments, the invention provides a biobased polymer, synthetic rubber, resin, or chemical comprising one or more bioderived 30 butadiene or bioderived butadiene intermediate produced by a non-naturally occurring microorganism of the invention or produced using a method disclosed herein. Propylene is a chemical commonly used in many commercial and industrial applications. Non-limiting examples of such applications include production of polymers, polymer 73 intermediates and chemicals, such as polypropylene, acrylic acid, butanol, butanediol, acrylonitrile, propylene oxide, isopropanol and cumene. Moreover, these propylene derivatives, such as polypropylene, are used in the production of a wide range of products including plastics, such as injection moulding, and fibers, such as carpets. Accordingly, in 5 some embodiments, the invention provides a biobased polymer, polymer intermediate, or chemical comprising one or more bioderived propylene or bioderived propylene intermediate produced by a non-naturally occurring microorganism of the invention or produced using a method disclosed herein. 1,3-Butanediol is a chemical commonly used in many commercial and industrial 10 applications. Non-limiting examples of such applications include its use as an organic solvent for food flavoring agents or as a hypoglycaemic agent and its use in the production of polyurethane and polyester resins . Moreover, optically active 1,3-butanediol is also used in the synthesis of biologically active compounds and liquid crystals. Still further, 1,3-butanediol can be used in commercial production of 1,3-butadiene, a compound used 15 in the manufacture of synthetic rubbers (e.g., tires), latex, and resins. Accordingly, in some embodiments, the invention provides a biobased organic solvent, hypoglycaemic agent, polyurethane, polyester resin, synthetic rubber, latex, or resin comprising one or more bioderived 1,3-butanediol or bioderived 1,3-butanediolinternediate produced by a non-naturally occurring microorganism of the invention or produced using a method 20 disclosed herein. Crotyl alcohol is a chemical commonly used in many commercial and industrial applications. Non-limiting examples of such applications include production of crotyl halides, esters, and ethers, which in turn are chemical are chemical intermediates in the production of monomers, fine chemicals, such as sorbic acid, trimethylhydroquinone, 25 crotonic acid and 3-methoxybutanol, agricultural chemicals, and pharmaceuticals. Crotyl alcohol can also be used as a precursor in the production of 1,3-butadiene. Accordingly, in some embodiments, the invention provides a biobased monomer, fine chemical, agricultural chemical, or pharmaceutical comprising one or more bioderived crotyl alcohol or bioderived crotyl alcohol intermediate produced by a non-naturally occurring 30 microorganism of the invention or produced using a method disclosed herein. 3-Buten-1-ol is a chemical commonly used in many commercial and industrial applications. Non-limiting examples of such applications include production of pharmaceuticals, agrochemicals, perfumes and resins. Accordingly, in some 74 embodiments, the invention provides a biobased pharmaceutical, agrochemical, perfume or resin comprising one or more bioderived 3-buten-1-ol or bioderived 3-buten-l-ol intermediate produced by a non-naturally occurring microorganism of the invention or produced using a method disclosed herein. 5 As used herein, the term "bioderived" means derived from or synthesized by a biological organism and can be considered a renewable resource since it can be generated by a biological organism. Such a biological organism, in particular the microbial organisms of the invention disclosed herein, can utilize feedstock or biomass, such as, sugars or carbohydrates obtained from an agricultural, plant, bacterial, or animal source. 10 Alternatively, the biological organism can utilize atmospheric carbon. As used herein, the term "biobased" means a product as described above that is composed, in whole or in part, of a bioderived compound of the invention. A biobased or bioderived product is in contrast to a petroleum derived product, wherein such a product is derived from or synthesized from petroleum or a petrochemical feedstock. 15 In some embodiments, the invention provides polyurethane, polymer or co-polymer comprising bioderived 2,4-pentadienoate or bioderived 2,4-pentadienoate pathway intermediate, wherein the bioderived 2,4-pentadienoate or bioderived 2,4-pentadienoate pathway intermediate includes all or part of the 2,4-pentadienoate or 2,4-pentadienoate pathway intermediate used in the production of polyurethane, polymer or co-polymer. 20 Thus, in some aspects, the invention provides a biobased polyurethane, polymer or co polymer comprising at least 2%, at least 3%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98% or 100% bioderived 2,4-pentadienoate or bioderived 2,4-pentadienoate pathway intermediate as 25 disclosed herein. Additionally, in some aspects, the invention provides a biobased polyurethane, polymer or co-polymer wherein the 2,4-pentadienoate or 2,4-pentadienoate pathway intermediate used in its production is a combination of bioderived and petroleum derived 2,4-pentadienoate or 2,4-pentadienoate pathway intermediate. For example, a biobased polyurethane, polymer or co-polymer can be produced using 50% bioderived 30 2,4-pentadienoate and 50% petroleum derived 2,4-pentadienoate or other desired ratios such as 60%/40%, 70 0 /30%, 80%/20%, 90%/10%, 95%1/5%, 100%/0%, 40%/60%, 30%/70%, 20%/80%, 10%/90% of bioderived/petroleum derived precursors, so long as at least a portion of the product comprises a bioderived product produced by the microbial 75 organisms disclosed herein. It is understood that methods for producing polyurethane, polymer or co-polymer using the bioderived 2,4-pentadienoate or bioderived 2,4 pentadienoate pathway intermediate of the invention are well known in the art. In some embodiments, the invention provides polymer, synthetic rubber, resin, or 5 chemical comprising bioderived butadiene or bioderived butadiene pathway intermediate, wherein the bioderived butadiene or bioderived butadiene pathway intermediate includes all or part of the butadiene or butadiene pathway intermediate used in the production of polymer, synthetic rubber, resin, or chemical. Thus, in some aspects, the invention provides a biobased polymer, synthetic rubber, resin, or chemical comprising at least 2%, 10 at least 3%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98% or 100% bioderived butadiene or bioderived butadiene pathway intermediate as disclosed herein. Additionally, in some aspects, the invention provides a biobased polymer, synthetic rubber, resin, or chemical wherein the 15 butadiene or butadiene pathway intermediate used in its production is a combination of bioderived and petroleum derived butadiene or butadiene pathway intermediate. For example, a biobased polymer, synthetic rubber, resin, or chemical can be produced using 50% bioderived butadiene and 50% petroleum derived butadiene or other desired ratios such as 60%/40%, 700/o/30%, 800/o/20%, 90%/I 0%, 95%/5%, 1000/o/0%, 40%/60%, 20 30%/70%, 20%/80%, 10%/90% of bioderived/petroleum derived precursors, so long as at least a portion of the product comprises a bioderived product produced by the microbial organisms disclosed herein, It is understood that methods for producing polymer, synthetic rubber, resin, or chemical using the bioderived butadiene or bioderived butadiene pathway intermediate of the invention are well known in the art. 25 In some embodiments, the invention provides polymer, polymer intermediate, or chemical comprising bioderived propylene or bioderived propylene pathway intermediate, wherein the bioderived propylene or bioderived propylene pathway intermediate includes all or part of the propylene or propylene pathway intermediate used in the production of polymer, polymer intermediate, or chemical. Thus, in some aspects, the invention 30 provides a biobased polymer, polymer intermediate, or chemical comprising at least 2%, at least 3%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98% or 100% bioderived propylene or bioderived propylene 76 pathway intermediate as disclosed herein. Additionally, in some aspects, the invention provides a biobased polymer, polymer intermediate, or chemical wherein the propylene or propylene pathway intermediate used in its production is a combination of bioderived and petroleum derived propylene or propylene pathway intermediate. For example, a biobased 5 polymer, polymer intermediate, or chemical can be produced using 50% bioderived propylene and 50% petroleum derived propylene or other desired ratios such as 60%/40%, 70%/30%, 80%/20%, 90%/10%, 95%/5%, 100%/0%, 40%/60%, 300/o/70%, 20%/80%, 10%/90% of bioderived/petroleum derived precursors, so long as at least a portion of the product comprises a bioderived product produced by the microbial organisms disclosed 10 herein. It is understood that methods for producing polymer, polymer intermediate, or chemical using the bioderived propylene or bioderived propylene pathway intermediate of the invention are well known in the art. In some embodiments, the invention provides organic solvent, hypoglycaemic agent, polyurethane, polyester resin, synthetic rubber, latex, or resin comprising bioderived 1,3 15 butanediol or bioderived 1,3-butanediol pathway intermediate, wherein the bioderived 1,3 butanediol or bioderived 1,3-butanediol pathway intermediate includes all or part of the 1,3-butanediol or 1,3-butanediol pathway intermediate used in the production of organic solvent, hypoglycaemic agent, polyurethane, polyester resin, synthetic rubber, latex, or resin. Thus, in some aspects, the invention provides a biobased organic solvent, 20 hypoglycaemic agent, polyurethane, polyester resin, synthetic rubber, latex, or resin comprising at least 2%, at least 3%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98% or 100% bioderived 1,3 butanediol or bioderived 1,3-butanediol pathway intermediate as disclosed herein. 25 Additionally, in some aspects, the invention provides a biobased organic solvent, hypoglycaemic agent, polyurethane, polyester resin, synthetic rubber, latex, or resin wherein the 1,3-butanediol or 1,3-butanediol pathway intermediate used in its production is a combination of bioderived and petroleum derived 1,3-butanediol or 1,3-butanediol pathway intermediate. For example, a biobased organic solvent, hypoglycaemic agent, 30 polyurethane, polyester resin, synthetic rubber, latex, or resin can be produced using 50% bioderived 1,3-butanediol and 50% petroleum derived 1,3-butanediol or other desired ratios such as 60%/40%, 700/o/30%, 800/o/20%, 900//10%, 95%/5%, 100%/0%, 40%/160%, 30%/70%, 200/o/80%, 100/o/90% of bioderived/petroleum derived precursors, so long as at least a portion of the product comprises a bioderived product produced by the microbial 77 organisms disclosed herein. It is understood that methods for producing organic solvent, hypoglycaemic agent, polyurethane, polyester resin, synthetic rubber, latex, or resin using the bioderived 1,3-butanediol or bioderived 1,3-butanediol pathway intermediate of the invention are well known in the art. 5 In some embodiments, the invention provides monomer, fine chemical, agricultural chemical, or pharmaceutical comprising bioderived crotyl alcohol or bioderived crotyl alcohol pathway intermediate, wherein the bioderived crotyl alcohol or bioderived crotyl alcohol pathway intermediate includes all or part of the crotyl alcohol or crotyl alcohol pathway intermediate used in the production of monomer, fine chemical, agricultural 10 chemical, or pharmaceutical. Thus, in some aspects, the invention provides a biobased monomer, fine chemical, agricultural chemical, or pharmaceutical comprising at least 2%, at least 3%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98% or 100% bioderived crotyl alcohol or bioderived 15 crotyl alcohol pathway intermediate as disclosed herein. Additionally, in some aspects, the invention provides a biobased monomer, fine chemical, agricultural chemical, or pharmaceutical wherein the crotyl alcohol or crotyl alcohol pathway intermediate used in its production is a combination of bioderived and petroleum derived crotyl alcohol or crotyl alcohol pathway intermediate. For example, a biobased monomer, fine chemical, 20 agricultural chemical, or pharmaceutical can be produced using 50% bioderived crotyl alcohol and 50% petroleum derived crotyl alcohol or other desired ratios such as 60%/40%, 70%/30%, 80%/20%, 90%/10%, 95%/5%, 100%/0%, 40%/60%, 30%/70%, 20%/80%, 10%/90% of bioderived/petroleum derived precursors, so long as at least a portion of the product comprises a bioderived product produced by the microbial 25 organisms disclosed herein. It is understood that methods for producing monomer, fine chemical, agricultural chemical, or pharmaceutical using the bioderived crotyl alcohol or bioderived crotyl alcohol pathway intermediate of the invention are well known in the art. In some embodiments, the invention provides pharmaceutical, agrochemical, perfume, or resin comprising bioderived 3-buten-1-ol or bioderived 3-buten-1-ol pathway 30 intermediate, wherein the bioderived 3-buten-l-ol or bioderived 3-buten-1-ol pathway intermediate includes all or part of the 3-buten- 1 -ol or 3-buten-1 -ol pathway intermediate used in the production of pharmaceutical, agrochemical, perfume, or resin. Thus, in some aspects, the invention provides a biobased pharmaceutical, agrochemical, perfume, or 78 resin comprising at least 2%, at least 3%, at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 98% or 100% bioderived 3 buten-1-ol or bioderived 3-buten-1-ol pathway intermediate as disclosed herein. 5 Additionally, in some aspects, the invention provides a biobased pharmaceutical, agrochemical, perfume, or resin wherein the 3-buten- 1 -ol or 3-buten- 1 -ol pathway intermediate used in its production is a combination of bioderived and petroleum derived 3-buten-1-ol or 3-buten-1-ol pathway intermediate. For example, a biobased pharmaceutical, agrochemical, perfume, or resin can be produced using 50% bioderived 3 10 buten-1-ol and 50% petroleum derived 3-buten-1-ol or other desired ratios such as 60%/40%, 70%/30%, 80%/20%, 90%/10%, 95//5%, 100%/0%, 40/60%, 30%/70%, 20%/80%, 10%/90% of bioderived/petroleum derived precursors, so long as at least a portion of the product comprises a bioderived product produced by the microbial organisms disclosed herein. It is understood that methods for producing pharmaceutical, 15 agrochemical, perfume, or resin using the bioderived 3-buten-1-ol or bioderived 3-buten 1-ol pathway intermediate of the invention are well known in the art. The culture conditions can include, for example, liquid culture procedures as well as fermentation and other large scale culture procedures. As described herein, particularly useful yields of the biosynthetic products of the invention can be obtained under anaerobic 20 or substantially anaerobic culture conditions. As described herein, one exemplary growth condition for achieving biosynthesis of 2,4 pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol includes anaerobic culture or fermentation conditions. In certain embodiments, the non naturally occurring microbial organisms of the invention can be sustained, cultured or 25 fermented under anaerobic or substantially anaerobic conditions. Briefly, anaerobic conditions refers to an environment devoid of oxygen. Substantially anaerobic conditions include, for example, a culture, batch fermentation or continuous fermentation such that the dissolved oxygen concentration in the medium remains between 0 and 10% of saturation. Substantially anaerobic conditions also includes growing or resting cells in 30 liquid medium or on solid agar inside a sealed chamber maintained with an atmosphere of less than 1% oxygen. The percent of oxygen can be maintained by, for example, sparging the culture with an N 2

/CO

2 mixture or other suitable non-oxygen gas or gases.

79 The culture conditions described herein can be scaled up and grown continuously for manufacturing of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. Exemplary growth procedures include, for example, fed-batch fermentation and batch separation; fed-batch fermentation and continuous separation, or 5 continuous fermentation and continuous separation. All of these processes are well known in the art. Fermentation procedures are particularly useful for the biosynthetic production of commercial quantities of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. Generally, and as with non-continuous culture procedures, the continuous and/or near-continuous production of 2,4-pentadienoate, butadiene, 10 propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol will include culturing a non naturally occurring 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol producing organism of the invention in sufficient nutrients and medium to sustain and/or nearly sustain growth in an exponential phase. Continuous culture under such conditions can include, for example, growth for I day, 2, 3, 4, 5, 6 or 7 days or more. 15 Additionally, continuous culture can include longer time periods of I week, 2, 3, 4 or 5 or more weeks and up to several months. Alternatively, organisms of the invention can be cultured for hours, if suitable for a particular application. It is to be understood that the continuous and/or near-continuous culture conditions also can include all time intervals in between these exemplary periods. It is further understood that the time of culturing the 20 microbial organism of the invention is for a sufficient period of time to produce a sufficient amount of product for a desired purpose. Fermentation procedures are well known in the art. Briefly, fermentation for the biosynthetic production of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol can be utilized in, for example, fed-batch fermentation and batch 25 separation; fed-batch fermentation and continuous separation, or continuous fermentation and continuous separation. Examples of batch and continuous fermentation procedures are well known in the art. In addition to the above fermentation procedures using the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol producers of the invention for 30 continuous production of substantial quantities of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol, the 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1 -ol producers also can be, for example, simultaneously subjected to chemical synthesis procedures to convert the product to other 80 compounds or the product can be separated from the fermentation culture and sequentially subjected to chemical or enzymatic conversion to convert the product to other compounds, if desired. To generate better producers, metabolic modeling can be utilized to optimize growth 5 conditions. Modeling can also be used to design gene knockouts that additionally optimize utilization of the pathway (see, for example, U.S. patent publications US 2002/0012939, US 2003/0224363, US 2004/0029149, US 2004/0072723, US 2003/0059792, US 2002/0168654 and US 2004/0009466, and U.S. Patent No. 7,127,379). Modeling analysis allows reliable predictions of the effects on cell growth of shifting the 10 metabolism towards more efficient production of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. One computational method for identifying and designing metabolic alterations favoring biosynthesis of a desired product is the OptKnock computational framework (Burgard et al., Biotechnol. Bioeng. 84:647-657 (2003)). OptKnock is a metabolic modeling and 15 simulation program that suggests gene deletion or disruption strategies that result in genetically stable microorganisms which overproduce the target product. Specifically, the framework examines the complete metabolic and/or biochemical network of a microorganism in order to suggest genetic manipulations that force the desired biochemical to become an obligatory byproduct of cell growth. By coupling biochemical 20 production with cell growth through strategically placed gene deletions or other functional gene disruption, the growth selection pressures imposed on the engineered strains after long periods of time in a bioreactor lead to improvements in performance as a result of the compulsory growth-coupled biochemical production. Lastly, when gene deletions are constructed there is a negligible possibility of the designed strains reverting to their wild 25 type states because the genes selected by OptKnock are to be completely removed from the genome. Therefore, this computational methodology can be used to either identify alternative pathways that lead to biosynthesis of a desired product or used in connection with the non-naturally occurring microbial organisms for further optimization of biosynthesis of a desired product. 30 Briefly, OptKnock is a term used herein to refer to a computational method and system for modeling cellular metabolism. The OptKnock program relates to a framework of models and methods that incorporate particular constraints into flux balance analysis (FBA) models. These constraints include, for example, qualitative kinetic information, 81 qualitative regulatory information, and/or DNA microarray experimental data. OptKnock also computes solutions to various metabolic problems by, for example, tightening the flux boundaries derived through flux balance models and subsequently probing the performance limits of metabolic networks in the presence of gene additions or deletions. 5 OptKnock computational framework allows the construction of model formulations that allow an effective query of the performance limits of metabolic networks and provides methods for solving the resulting mixed-integer linear programming problems. The metabolic modeling and simulation methods referred to herein as OptKnock are described in, for example, U.S. publication 2002/0168654, filed January 10, 2002, in International 10 Patent No. PCT/US02/00660, filed January 10, 2002, and U.S. publication 2009/0047719, filed August 10, 2007. Another computational method for identifying and designing metabolic alterations favoring biosynthetic production of a product is a metabolic modeling and simulation system termed SimPheny@. This computational method and system is described in, for 15 example, U.S. publication 2003/0233218, filed June 14, 2002, and in International Patent Application No. PCT/US03/18838, filed June 13, 2003. SimPheny@ is a computational system that can be used to produce a network model in silico and to simulate the flux of mass, energy or charge through the chemical reactions of a biological system to define a solution space that contains any and all possible functionalities of the chemical reactions 20 in the system, thereby determining a range of allowed activities for the biological system. This approach is referred to as constraints-based modeling because the solution space is defined by constraints such as the known stoichiometry of the included reactions as well as reaction thermodynamic and capacity constraints associated with maximum fluxes through reactions. The space defined by these constraints can be interrogated to determine 25 the phenotypic capabilities and behavior of the biological system or of its biochemical components. These computational approaches are consistent with biological realities because biological systems are flexible and can reach the same result in many different ways. Biological systems are designed through evolutionary mechanisms that have been restricted by 30 fundamental constraints that all living systems must face. Therefore, constraints-based modeling strategy embraces these general realities. Further, the ability to continuously impose further restrictions on a network model via the tightening of constraints results in a 82 reduction in the size of the solution space, thereby enhancing the precision with which physiological performance or phenotype can be predicted. Given the teachings and guidance provided herein, those skilled in the art will be able to apply various computational frameworks for metabolic modeling and simulation to design 5 and implement biosynthesis of a desired compound in host microbial organisms. Such metabolic modeling and simulation methods include, for example, the computational systems exemplified above as SimPheny@ and OptKnock. For illustration of the invention, some methods are described herein with reference to the OptKnock computation framework for modeling and simulation. Those skilled in the art will know 10 how to apply the identification, design and implementation of the metabolic alterations using OptKnock to any of such other metabolic modeling and simulation computational frameworks and methods well known in the art. The methods described above will provide one set of metabolic reactions to disrupt. Elimination of each reaction within the set or metabolic modification can result in a 15 desired product as an obligatory product during the growth phase of the organism. Because the reactions are known, a solution to the bilevel OptKnock problem also will provide the associated gene or genes encoding one or more enzymes that catalyze each reaction within the set of reactions. Identification of a set of reactions and their corresponding genes encoding the enzymes participating in each reaction is generally an 20 automated process, accomplished through correlation of the reactions with a reaction database having a relationship between enzymes and encoding genes. Once identified, the set of reactions that are to be disrupted in order to achieve production of a desired product are implemented in the target cell or organism by functional disruption of at least one gene encoding each metabolic reaction within the set. One 25 particularly useful means to achieve functional disruption of the reaction set is by deletion of each encoding gene. However, in some instances, it can be beneficial to disrupt the reaction by other genetic aberrations including, for example, mutation, deletion of regulatory regions such as promoters or cis binding sites for regulatory factors, or by truncation of the coding sequence at any of a number of locations. These latter 30 aberrations, resulting in less than total deletion of the gene set can be useful, for example, when rapid assessments of the coupling of a product are desired or when genetic reversion is less likely to occur.

83 To identify additional productive solutions to the above described bilevel OptKnock problem which lead to further sets of reactions to disrupt or metabolic modifications that can result in the biosynthesis, including growth-coupled biosynthesis of a desired product, an optimization method, termed integer cuts, can be implemented. This method proceeds 5 by iteratively solving the OptKnock problem exemplified above with the incorporation of an additional constraint referred to as an integer cut at each iteration. Integer cut constraints effectively prevent the solution procedure from choosing the exact same set of reactions identified in any previous iteration that obligatorily couples product biosynthesis to growth. For example, if a previously identified growth-coupled metabolic modification 10 specifies reactions 1, 2, and 3 for disruption, then the following constraint prevents the same reactions from being simultaneously considered in subsequent solutions. The integer cut method is well known in the art and can be found described in, for example, Burgard et al., Biotechnol. Prog. 17:791-797 (2001). As with all methods described herein with reference to their use in combination with the OptKnock computational framework for 15 metabolic modeling and simulation, the integer cut method of reducing redundancy in iterative computational analysis also can be applied with other computational frameworks well known in the art including, for example, SimPheny@. The methods exemplified herein allow the construction of cells and organisms that biosynthetically produce a desired product, including the obligatory coupling of 20 production of a target biochemical product to growth of the cell or organism engineered to harbor the identified genetic alterations. Therefore, the computational methods described herein allow the identification and implementation of metabolic modifications that are identified by an in silico method selected from OptKnock or SimPheny@. The set of metabolic modifications can include, for example, addition of one or more biosynthetic 25 pathway enzymes and/or functional disruption of one or more metabolic reactions including, for example, disruption by gene deletion. As discussed above, the OptKnock methodology was developed on the premise that mutant microbial networks can be evolved towards their computationally predicted maximum-growth phenotypes when subjected to long periods of growth selection. In 30 other words, the approach leverages an organism's ability to self-optimize under selective pressures. The OptKnock framework allows for the exhaustive enumeration of gene deletion combinations that force a coupling between biochemical production and cell growth based on network stoichiometry. The identification of optimal gene/reaction 84 knockouts requires the solution of a bilevel optimization problem that chooses the set of active reactions such that an optimal growth solution for the resulting network overproduces the biochemical of interest (Burgard et al., Biotechnol. Bioeng. 84:647-657 (2003)). 5 An in silicon stoichiometric model of E. coli metabolism can be employed to identify essential genes for metabolic pathways as exemplified previously and described in, for example, U.S. patent publications US 2002/0012939, US 2003/0224363, US 2004/0029149, US 2004/0072723, US 2003/0059792, US 2002/0168654 and US 2004/0009466, and in U.S. Patent No. 7,127,379. As disclosed herein, the OptKnock 10 mathematical framework can be applied to pinpoint gene deletions leading to the growth coupled production of a desired product. Further, the solution of the bilevel OptKnock problem provides only one set of deletions. To enumerate all meaningful solutions, that is, all sets of knockouts leading to growth-coupled production formation, an optimization technique, termed integer cuts, can be implemented. This entails iteratively solving the 15 OptKnock problem with the incorporation of an additional constraint referred to as an integer cut at each iteration, as discussed above. As disclosed herein, a nucleic acid encoding a desired activity of a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol pathway can be introduced into a host organism. In some cases, it can be desirable to modify an activity of 20 a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten- 1-ol pathway enzyme or protein to increase production of 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-1-ol. For example, known mutations that increase the activity of a protein or enzyme can be introduced into an encoding nucleic acid molecule. Additionally, optimization methods can be applied to increase the 25 activity of an enzyme or protein and/or decrease an inhibitory activity, for example, decrease the activity of a negative regulator. One such optimization method is directed evolution. Directed evolution is a powerful approach that involves the introduction of mutations targeted to a specific gene in order to improve and/or alter the properties of an enzyme. Improved and/or altered enzymes can 30 be identified through the development and implementation of sensitive high-throughput screening assays that allow the automated screening of many enzyme variants (for example, >104). Iterative rounds of mutagenesis and screening typically are performed to afford an enzyme with optimized properties. Computational algorithms that can help to 85 identify areas of the gene for mutagenesis also have been developed and can significantly reduce the number of enzyme variants that need to be generated and screened. Numerous directed evolution technologies have been developed (for reviews, see Hibbert et al., Biomol.Eng 22:11-19 (2005); Huisman and Lalonde, In Biocatalysis in the pharmaceutical 5 and biotechnology industries pgs. 717-742 (2007), Patel (ed.), CRC Press; Otten and Quax. Biomol.Eng 22:1-9 (2005).; and Sen et al., Appl Biochem.Biotechnol 143:212-223 (2007)) to be effective at creating diverse variant libraries, and these methods have been successfully applied to the improvement of a wide range of properties across many enzyme classes. Enzyme characteristics that have been improved and/or altered by 10 directed evolution technologies include, for example: selectivity/specificity, for conversion of non-natural substrates; temperature stability, for robust high temperature processing; pH stability, for bioprocessing under lower or higher pH conditions; substrate or product tolerance, so that high product titers can be achieved; binding (Km), including broadening substrate binding to include non-natural substrates; inhibition (Ki), to remove 15 inhibition by products, substrates, or key intermediates; activity (kcat), to increases enzymatic reaction rates to achieve desired flux; expression levels, to increase protein yields and overall pathway flux; oxygen stability, for operation of air sensitive enzymes under aerobic conditions; and anaerobic activity, for operation of an aerobic enzyme in the absence of oxygen. 20 A number of exemplary methods have been developed for the mutagenesis and diversification of genes to target desired properties of specific enzymes. Such methods are well known to those skilled in the art. Any of these can be used to alter and/or optimize the activity of a 2,4-pentadienoate, butadiene, propylene, 1,3-butanediol, crotyl alcohol or 3-buten-l-ol pathway enzyme or protein. Such methods include, but are not limited to 25 EpPCR, which introduces random point mutations by reducing the fidelity of DNA polymerase in PCR reactions (Pritchard et al., J Theor.Biol. 234:497-509 (2005)); Error prone Rolling Circle Amplification (epRCA), which is similar to epPCR except a whole circular plasmid is used as the template and random 6-mers with exonuclease resistant thiophosphate linkages on the last 2 nucleotides are used to amplify the plasmid followed 30 by transformation into cells in which the plasmid is re-circularized at tandem repeats (Fujii et al., Nucleic Acids Res. 32:e 145 (2004); and Fujii et al., Nat. Protoc. 1:2493-2497 (2006)); DNA or Family Shuffling, which typically involves digestion of two or more variant genes with nucleases such as Dnase 1 or EndoV to generate a pool of random fragments that are reassembled by cycles of annealing and extension in the presence of 86 DNA polymerase to create a library of chimeric genes (Stemmer, Proc Natl A cad Sci USA 91:10747-10751 (1994); and Stemmer, Nature 370:389-391 (1994)); Staggered Extension (StEP), which entails template priming followed by repeated cycles of 2 step PCR with denaturation and very short duration of annealing/extension (as short as 5 sec) (Zhao et al., 5 Nat. Biotechnol. 16:258-261 (1998)); Random Priming Recombination (RPR), in which random sequence primers are used to generate many short DNA fragments complementary to different segments of the template (Shao et al., Nucleic Acids Res 26:681-683 (1998)). Additional methods include Heteroduplex Recombination, in which linearized plasmid DNA is used to form heteroduplexes that are repaired by mismatch repair (Volkov et al, 10 Nucleic Acids Res. 27:e18 (1999); and Volkov et al., Methods Enzymol. 328:456-463 (2000)); Random Chimeragenesis on Transient Templates (RACHITT), which employs Dnase I fragmentation and size fractionation of single stranded DNA (ssDNA) (Coco et al., Nat. Biotechnol. 19:354-359 (2001)); Recombined Extension on Truncated templates (RETT), which entails template switching of unidirectionally growing strands from 15 primers in the presence of unidirectional ssDNA fragments used as a pool of templates (Lee et al., J. Molec. Catalysis 26:119-129 (2003)); Degenerate Oligonucleotide Gene Shuffling (DOGS), in which degenerate primers are used to control recombination between molecules; (Bergquist and Gibbs, Methods Mol.Biol 352:191-204 (2007); Bergquist et al., Biomol.Eng 22:63-72 (2005); Gibbs et al., Gene 271:13-20 (2001)); 20 Incremental Truncation for the Creation of Hybrid Enzymes (ITCHY), which creates a combinatorial library with 1 base pair deletions of a gene or gene fragment of interest (Ostermeier et al., Proc. Natl. Acad. Sci. USA 96:3562-3567 (1999); and Ostermeier et al., Nat. Biotechnol. 17:1205-1209 (1999)); Thio-Incremental Truncation for the Creation of Hybrid Enzymes (THIO-ITCHY), which is similar to ITCHY except that phosphothioate 25 dNTPs are used to generate truncations (Lutz et al., Nucleic Acids Res 29:E16 (2001)); SCRATCHY, which combines two methods for recombining genes, ITCHY and DNA shuffling (Lutz et al., Proc. NatL. Acad. Sci. USA 98:11248-11253 (2001)); Random Drift Mutagenesis (RNDM), in which mutations made via epPCR are followed by screening/selection for those retaining usable activity (Bergquist et al., Biomol. Eng. 30 22:63-72 (2005)); Sequence Saturation Mutagenesis (SeSaM), a random mutagenesis method that generates a pool of random length fragments using random incorporation of a phosphothioate nucleotide and cleavage, which is used as a template to extend in the presence of "universal" bases such as inosine, and replication of an inosine-containing complement gives random base incorporation and, consequently, mutagenesis (Wong et 87 al., Biotechnol. J. 3:74-82 (2008); Wong et al., Nucleic Acids Res. 32:e26 (2004); and Wong et al., Anal. Biochem. 341:187-189 (2005)); Synthetic Shuffling, which uses overlapping oligonucleotides designed to encode "all genetic diversity in targets" and allows a very high diversity for the shuffled progeny (Ness et al., Nat. Biotechnol. 5 20:1251-1255 (2002)); Nucleotide Exchange and Excision Technology NexT, which exploits a combination of dUTP incorporation followed by treatment with uracil DNA glycosylase and then piperidine to perform endpoint DNA fragmentation (Muller et al., Nucleic Acids Res, 33:el 17 (2005)). Further methods include Sequence Homology-Independent Protein Recombination 10 (SHIPREC), in which a linker is used to facilitate fusion between two distantly related or unrelated genes, and a range of chimeras is generated between the two genes, resulting in libraries of single-crossover hybrids (Sieber et al., Nat. Biotechnol. 19:456-460 (2001)); Gene Site Saturation MutagenesisTm (GSSMTM), in which the starting materials include a supercoiled double stranded DNA (dsDNA) plasmid containing an insert and two primers 15 which are degenerate at the desired site of mutations (Kretz et al., Methods Enzymol. 388:3-11 (2004)); Combinatorial Cassette Mutagenesis (CCM), which involves the use of short oligonucleotide cassettes to replace limited regions with a large number of possible amino acid sequence alterations (Reidhaar-Olson et al. Methods Enzymol. 208:564-586 (1991); and Reidhaar-Olson et al. Science 241:53-57 (1988)); Combinatorial Multiple 20 Cassette Mutagenesis (CMCM), which is essentially similar to CCM and uses epPCR at high mutation rate to identify hot spots and hot regions and then extension by CMCM to cover a defined region of protein sequence space (Reetz et al., Angew. Chem. Int. Ed Engl. 40:3589-3591 (2001)); the Mutator Strains technique, in which conditional ts mutator plasmids, utilizing the mutD5 gene, which encodes a mutant subunit of DNA polymerase 25 III, to allow increases of 20 to 4000-X in random and natural mutation frequency during selection and block accumulation of deleterious mutations when selection is not required (Selifonova et al., Appl. Environ. Microbiol. 67:3645-3649 (2001)); Low et al., J. Mol. Biol. 260:359-3680 (1996)). Additional exemplary methods include Look-Through Mutagenesis (LTM), which is a 30 multidimensional mutagenesis method that assesses and optimizes combinatorial mutations of selected amino acids (Rajpal et al., Proc. Natl. Acad. Sci. USA 102:8466 8471 (2005)); Gene Reassembly, which is a DNA shuffling method that can be applied to multiple genes at one time or to create a large library of chimeras (multiple mutations) of a 88 single gene (Tunable GeneReassemblyTM (TGRT4) Technology supplied by Verenium Corporation), in Silico Protein Design Automation (PDA), which is an optimization algorithm that anchors the structurally defined protein backbone possessing a particular fold, and searches sequence space for amino acid substitutions that can stabilize the fold 5 and overall protein energetics, and generally works most effectively on proteins with known three-dimensional structures (Hayes et al., Proc. Nat. Acad. Sci. USA 99:15926 15931 (2002)); and Iterative Saturation Mutagenesis (ISM), which involves using knowledge of structure/function to choose a likely site for enzyme improvement, performing saturation mutagenesis at chosen site using a mutagenesis method such as 10 Stratagene QuikChange (Stratagene; San Diego CA), screening/selecting for desired properties, and, using improved clone(s), starting over at another site and continue repeating until a desired activity is achieved (Reetz et al., Nat. Protoc. 2:891-903 (2007); and Reetz et al., Angew. Chem. Int. Ed EngL. 45:7745-7751 (2006)). Any of the aforementioned methods for mutagenesis can be used alone or in any 15 combination. Additionally, any one or combination of the directed evolution methods can be used in conjunction with adaptive evolution techniques, as described herein. It is understood that modifications which do not substantially affect the activity of the various embodiments of this invention are also provided within the definition of the invention provided herein. Accordingly, the following examples are intended to illustrate 20 but not limit the present invention. EXAMPLE I Pathways for producing 2,4-pentadienoate, 3-buten-1-ol and butadiene from 2 aminopentanoate, 2-oxoadipate and glutaryl-CoA Several routes to 2,4-pentadienoate, 3-buten-1-ol and butadiene, are depicted in Figure 1. 25 Starting metabolites include 2-oxoadipate, glutaryl-CoA, and 5-aminopentanoate. These routes are catalyzed by one or more of the following enzymes: 2-aminoadipate decarboxylase, 5-aminopentanoate reductase, 5-aminopent-2-enoate aminotransferase, dehydrogenase or amine oxidase, 2-oxoadipate decarboxylase, glutarate semialdehyde reductase, 5-hydroxyvalerate dehydrogenase, 5-hydroxypent-2-enoate dehydratase, 2 30 aminoadipate aminotransferase, dehydrogenase or amine oxidase, 5-aminopentanoate aminotransferase, dehydrogenase or amine oxidase, 5-aminopent-2-enoate deaminase, 5 hydroxypent-2-enoate reductase, 5-hydroxyvaleryl-CoA transferase and/or synthetase, 5- 89 hydroxypentanoyl-CoA dehydrogenase, 5-hydroxypent-2-enoyl-CoA dehydratase, 2,4 pentadienoyl-CoA transferase, synthetase or hydrolase, 5-hydroxypent-2-enoyl-CoA transferase or synthetase, 5-hydroxyvaleryl-CoA dehydratase/dehydrogenase, 2 oxoadipate dehydrogenase, 2-oxoadipate:ferridoxin oxidoreductase, 2-oxoadipate formate 5 lyase, glutaryl-CoA reductase, 2,4-pentadienoate decarboxylase, 5-hydroxypent-2-enoate decarboxylase, 3-buten-1-ol dehydratase and 5-hydroxyvalerate decarboxylase. Glutaryl-CoA is an intermediate in the degradation of numerous metabolites including benzoyl-CoA, lysine and tryptophan. Glutaryl-CoA can also be biosynthesized by means of, for example, the pathway shown in Figure 2. Glutaryl-CoA can be converted to 2,4 10 pentadienoate in five or more enzymatic steps. In the first step, glutaryl-CoA is reduced to glutarate semialdehyde by glutaryl-CoA reductase (step S). Further reduct ion to 5 hydroxyvalerte is catalyzed by an aldehyde reductase enzyme (step E). 5-Hydroxyvalerate is subsequently activated to 5-hydroxyvaleryl-CoA by a CoA transferase or synthetase in step L. The conversion of 5-hydroxyvaleryl-CoA to 2,4-pentadienoyl-CoA is catalyzed by 15 a bifunctional enzyme with dehydratase and dehydrogenase activity (step Q). Alternately, the reaction is catalyzed in two steps by separate enzymes (step M, N). 2,4-Pentadienoate is formed by removal of the CoA moiety by a CoA transferase, synthetase or hydrolase (step 0). 2,4-Pentadienoate or 2,4-pentadienoyl-CoA can be further converted to butadiene by a number of pathways shown in Figure 6. Alternate pathways for converting 5 20 hydroxyvalerate to 2,4-pentadienoate and butadiene are also shown. The 5 hydroxyvalerate intermediate can also be converted to 3-buten- 1-ol in one or more enzymatic steps. Direct conversion of 5-hydroxyvalerate to 3-buten- 1 -ol is catalyzed by an alkene-forming decarboxylase (step W). Indirect conversion entails oxidation of 5 hydroxyvalerate to 5-hydroxypent-2-enoate, followed by decarboxylation to 3-buten-1-ol 25 (steps F and U). The 3-buten- I -ol can be isolated as a product, or further dehydrated to form butadiene. The dehydration proceeds via an enzymatic or catalytic reaction. Another starting metabolite for the pathways shown in Figure 1 is 5-aminopentanoate. 5 Aminopentanoate is an intermediate formed during lysine, ornithine and proline degradation. An aminotransferase, dehydrogenase or amine oxidase is required to convert 30 5-aminopentanoate to glutarate semialdehyde. Glutarate semialdehyde is then converted to 2,4-pentadienoate, 3-buten- 1 -ol or butadiene as described above. Alternately, 5 aminopentanoate is oxidized to 5-aminopent-2-enoate by an enoic acid reductase (step B). Deamination of 5-aminopent-2-enoate yields 2,4-pentadienoate. In yet another 90 embodiment, 5-aminopent-2-enoate is first converted to its corresponding aldehyde, 5 hydroxypent-2-enoate by an aminotransferase, dehydrogenase or amine oxidase. 5 Hydroxypent-2-enoate is then dehydrated to 2,4-pentadienoate directly (step G) or via a CoA intermediate (steps P, N, Q). 5 2-Aminoadipate and 2-oxoadipate (also called alpha-ketoadipate) are intermediates of lysine metabolism in organisms such as Saccharomyces cerevisiae. 2-Oxoadipate is also an intermediate of coenzyme B biosynethesis, where it is formed from alpha-ketoglutarate and acetyl-CoA by the enzymes homocitrate synthase, homoaconitase, and homoisocitrate dehydrogenase. 2-Oxoadipate and 2-aminoadipate are interconverted by aminotransferase, 10 dehydrogenase or amine oxidase enzymes. Decarboxylation of 2-oxoadipate by a keto acid decarboxylase yields glutarate semialdehyde (step D). Alternately, an acylating decarboxylase with alpha-ketoadipate dehydrogenase activity forms glutaryl-CoA from 2 oxoadipate (step R). Decarboxylation of 2-aminoadipate by an amino acid decarboxylase yields 5-aminopentanoate. Further transformation of the glutaryl-CoA, glutarate 15 semialdehyde or 5-aminopentanoate intermediates to 2,4-pentadienoate, 3-buten-1-ol or butadiene proceeds as shown in Figure 1 and described previously. Enzyme candidates for the reactions shown in Figure 1 are described in Example VII Example II. Pathway for producing glutaryl-CoA from acetyl-CoA 20 Figure 2 shows a carbon efficient pathway for converting two molecules of acetyl-CoA to glutaryl-CoA. In the first step, acetoacetyl-CoA is formed by the condensation of two molecules of acetyl-CoA by acetoacetyl-CoA thiolase, a beta-ketothiolase enzyme. Acetoacetyl-CoA can alternately be formed from malonyl-CoA and acetyl-CoA by acetoacetyl-CoA synthase. The 3-keto group of acetoacetyl-CoA is then reduced and 25 dehydrated to form crotonyl-CoA. Glutaryl-CoA is formed from the reductive carboxylation of crotonyl-CoA. Enzymes and gene candidates for converting acetoacetyl CoA to glutaryl-CoA are described in further detail in Example VII. Example III. Pathway for producing 2,4-pentadienoate from propionyl-CoA 30 This example describes a pathway for converting propionyl-CoA to 2,4-pentadienoate, shown in Figure 3. Enzymes include: 3-oxopentanoyl-CoA thiolase or synthase, 3- 91 oxopentanoyl-CoA reductase, 3-hydroxypentanoyl-CoA dehydratase, pent-2-enoyl-CoA isomerase, pent-3-enoyl-CoA dehydrogenase, one or more of 2,4-pentadienoyl-CoA hydrolase, transferase or synthetase and pent-2-enoyl-CoA dehydrogenase. Propionyl-CoA is formed as a metabolic intermediate in numerous biological pathways 5 including the 3-hydroxypropionate/4-hydroxybutyrate and 3-hydroxypropionate cycles of

CO

2 fixation, conversion of succinate or pyruvate to propionate, glyoxylate assimilation and amino acid degradation. In the pathways of Figure 3, propionyl-CoA is further converted to 2,4-pentadienoate. In the first step of the pathway, propionyl-CoA and acetyl CoA are condensed to 3-oxopentanoyl-CoA by 3-oxopentanoyl-CoA thiolase. Alternately, 10 propionyl-CoA and malonyl-CoA are condensed by an enzyme with 3-oxopentanoyl-CoA synthase activity. Alternately, the 3-oxopentanoyl-CoA intermediate can be formed in two steps by first converting propionyl-CoA and malonyl-ACP to 3-oxopentanoyl-ACP, then converting the ACP to the CoA. 3-Oxopentanoyl-CoA is then reduced to 3 hydroxypentanoyl-CoA, and subsequently dehydrated to pent-2-enoyl-CoA by a 3 15 oxoacyl-CoA reductase and 3-hydroxyacyl-CoA dehydratase, resepectively (steps B, C). A delta-isomerase shifts the double bond from the 2- to the 3- position, forming pent-3 enoyl-CoA, the substrate for pent-3-enoyl-CoA dehydrogenase (steps D and E). Together the enzymes catalyzing steps B, C, D and E participate in the reverse direction in 5 aminovalerate utilizing organisms such as Clostridium aminovalericum. Alternately the 20 pent-2-enoyl-CoA intermediate is oxidized to 2,4-pentadienoyl-CoA by a pent-2-enoyl CoA dehydrogenase. In the final step of the pathway, 2,4-pentadienoyl-CoA is converted to its corresponding acid by a CoA hydrolase, transferse or synthetase (step F). 2,4 Pentadiene can be isolated as a product, or 2,4-Pentadienoate or 2,4-pentadienoyl-CoA can be further converted to butadiene as depicted in Figure 6. Enzymes and gene candidates 25 for converting propionyl-CoA to 2,4-pentadienoate are described in further detail in Example VII. Example IV. Pathway for synthesizing 1,3-butanediol from 3-hydroxypropionyl-CoA. This example describes a pathway for converting 3-hydroxypropionyl-CoA to 1,3 30 butanediol, shown in Figure 4. Enzymes include: 3-oxo-5-hydroxypentanoyl-CoA thiolase or a 3-oxo-5-hydroxypentanoyl-CoA synthase, 3-oxo-5-hydroxypentanoate decarboxylase, 3-oxobutanol reductase and one or more of 3-oxo-5-hydroxypentanoyl-CoA hydrolase, transferase or synthetase.

92 3-Hydroxypropionyl-CoA is an intermediate of the 3-hydroxypropionate/4 hydroxybutyrate CO 2 fixation cycle of autotrophs and a related 3-hydroxypropionate cycle discovered in phototrophic bacteria (Berg et al, Science 318(5857):1782-6 (2007); Strauss and Fuchs, EurJBiochem 215(3):633-43 (1993)). In the pathway to 1,3-butanediol, 3 5 hydroxypropionyl-CoA and acetyl-CoA are condensed by a 3-oxo-5-hydroxypentanoyl CoA thiolase to form 3-oxo-5-hydroxypentanoyl-CoA (step A). Alternately, the 3-oxo-5 hydroxypentanoyl-CoA intermediate is formed from 3-HP-CoA and malonyl-CoA by a 3 oxo-5-hydroxypentanoyl-CoA synthase. Removal of the CoA moiety by a CoA synthetase, transferase or hydrolase yields 3-oxo-5-hydroxypentanoate (step B). 10 Decarboxylation of 3-oxo-5-hydroxypentanoate to 3-oxobutanol is catalyzed by a keto acid decarboxylase (step C). In the final step of the pathway 3-oxobutanol is reduced to 1,3-butanol by an alcohol dehydrogenase or ketone reductase. Enzymes and gene candidates are described in further detail in Example VII. Example V. 15 Pathways for the formation of 1,3-butanediol, 3-buten-1-ol and butadiene from pyruvate and acetaldehyde. This example describes pathways for converting pyruvate and acetaldehyde to 1,3 butanediol, 3-buten-1-ol and butadiene. The pathways are shown in Figure 5. Relevant enzymes include: 4-hydroxy-2-oxovalerate aldolase, 4-hydroxy-2-oxovalerate 20 dehydratase, 2-oxopentenoate decarboxylase, 3-buten-1-al reductase, 3-buten-1-ol dehydratase, 4-hydroxy-2-oxovalerate decarboxylase, 3-hydroxybutanal reductase, 4 hydroxy-2-oxopentanoate dehydrogenase, 4-hydroxy-2-oxopentanoate:ferredoxin oxidoreductase, 3-hydroxybutyryl-CoA reductase (aldehyde forming), 3-hydroxybutyryl CoA hydrolase, 3-hydroxybutyryl-CoA transferase or 3-hydroxybutyryl-CoA synthetase, 25 3-hydroxybutyrate reductase and 3-hydroxybutyryl-CoA reductase (alcohol forming). Step E can also be catalyzed via chemical dehydration. The conversion of pyruvate and acetaldehyde to 3-buten-1-ol is accomplished in four enzymatic steps. Pyruvate and acetaldehyde are first condensed to 4-hydroxy-2 oxovalerate by 4-hydroxy-2-ketovalerate aldolase (Step A of Figure 5). The 4-hydroxy-2 30 oxovalerate product is subsequently dehydrated to 2-oxopentenoate (Step B of Figure 5). Decarboxylation of 2-oxopentenoate yields 3-buten-1-al (step C), which is further reduced to 3-buten-1-ol by an alcohol dehydrogenase (Step D). Further dehydration of the 3-buten 1-ol product to butadiene is performed by an enzyme or chemical catalyst.

93 The 4-hydroxy-2-oxovalerate intermediate can also be converted to 1,3-butanediol in two or more enzymatic steps. In one embodiment, 4-hydroxy-2-oxovalerate is decarboxylated to 3-hydroxybutanal (step F) and reduced to form 1,3-butanediot (step G). Alternately, 4 hydroxy-2-oxovalerate is converted to 3-hydroxybutyryl-CoA by an acylating and 5 decarboxylating oxidoreductase or formate lyase (step H). The 3-hydroxybutyryl-CoA intermediate is further reduced to 3-hydroxybutanal in one or two enzymatic steps, by either an aldehyde-forming acyl-CoA reductase (step I) or the combined reaction of a 3 hydroxybutyryl-CoA hydrolase, transferase or synthetase and a 3-hydroxybutyrate reductase (steps J, K). 3-Hydroxybutanal is further reduced to 1,3-butanediol by 3 10 hydroxybutanal reductase (step G). In another embodiment, the 3-hydroxybutyryl-CoA intermediate is directly converted to 1,3-butanediol by an alcohol-forming bifunctional aldehyde/alcohol dehydrogenase (step L). Enzymes and gene candidates are described in further detail in Example VII. Example VI. 15 Pathways for converting 2,4-pentadienoate or 2,4-pentadienoyl-CoA to butadiene. Figures 1 and 3 show pathways for forming 2,4-pentadienoate or 2,4-pentadienoyl-CoA from common metabolic precursors. Figure 6 shows pathways for further converting 2,4 pentadienoate or 2,4-pentadienoyl-CoA to butadiene. 2,4-Pentadienoate is converted to butadiene by several alternate pathways. One route is direct decarboxylation, shown in 20 step G. Alternately, the acid moiety is reduced to an aldehyde by a carboxylic acid reductase enzyme (step A). Decarbonylation of the penta-2,4-dienal intermediate forms butadiene (step B). Steps H and E depict an alternate pathway wherein 2,4-pentadienoate is first activated to 2,4-pentadienoyl-phosphate by a kinase, and subsequently reduced to penta-2,4-dienal by a phosphate reductase. 2,4-Pentadienoate and 2,4-pentadienoyl-CoA 25 are interconverted by a CoA transferase, hydrolase or synthetase. Reduction of 2,4 pentadienoyl-CoA to its corresponding aldehyde is catalyzed by an acylating aldehyde dehydrogenase (step C). Alternately, the CoA moiety is exchanged for a phosphate by a 2,4-pentadienoyl-CoA phosphotransferase (step D). The 2,4-pentadienoyl-phosphate or penta-2,4-dienal intermediates are further converted to butadiene as described previously. 30 Enzymes and gene candidates for the reactions shown in Figure 6 are described in further detail in Example VII.

94 Example VII. Enzyme candidates for the reactions shown in Figures 1-6 Label Function Step 1.1.1.a Oxidoreductase (oxo to alcohol) 1E,1K;2B; 3B; 4D;5D, 5G 1.1.1 .c Oxidoreductase (acyl-CoA to alcohol) 5L 1.2.1 .b Oxidoreductase (acyl-CoA to aldehyde) IS, 5I, 6C Oxidoreductase (2-oxo acid to acyl- 1R, 5H 1.2.1.c CoA) 1.2.1 .d Oxidoreductase (dephosphorylating) 6E 1.2.1 .e Oxidoreductase (acid to aldehyde) 5K, 6A 1.3.1.a Oxidoreducatse alkanee to alkene) 1B,1F,1M; 3E, 3G 1.4.1.a Oxidoreductase (amine to oxo) 1C,IH,1I 1.4.3.a Amine oxidase 1C,1H,1I Acyltransferase (transferring phosphate 6D 2.3.1 .a group to CoA; phosphotransacylase) 2.3.1 .b Beta-ketothiolase 2A,3A,4A 2.3.1Ld Formate C-acyltransferase 1R, 5H 2.3.1.e Synthase 2A, 3A, 4A 2.6.1.a Aminotransferase IC, 1H,1I 2.7.2.a Phosphotransferase (kinase) 6H 2.8.3.a CoA transferase 1L,1P,10; 3F; 4B; 5J; 6F 3.1.2.a CoA hydrolase 10; 3F; 4B; 5J; 6F 1A,1D,1T, 1U; 2D; 4C; 5C, 4.1.1.a Decarboxylase 5F; 6G 4.1.1 .b Decarboxylase, alkene forming 1W 4.1.99.a Decarbonylase 6B 4.1.3.a Lyase 5A 4.2.1.a Hydro-lyase 1G,1N, 1V; 2C, 3C; 5B, 5E 4.3.1.a Ammonia-lyase 1J 5.3.3.a Delta-isomerase 3D 6.2.1.a CoA synthetase 1L,1P,10; 3F; 4B; 5J; 6F N/A Bifunctional dehydratase/dehydrogenase 1Q 1.1.1.a Oxidoreductase (oxo to alcohol) 5 Several reactions shown in Figures 1-5 are catalyzed by alcohol dehydrogenase enzymes. These reactions include Steps E and K of Figure 1, Step B of Figure 2, Step B of Figure 3, Step D of Figure 4 and Steps D and G of Figure 5. Exemplary alcohol dehydrogenase enzymes are described in further detail below. The reduction of glutarate semialdehyde to 5-hydroxyvalerate by glutarate semialdehyde 10 reductase entails reduction of an aldehyde to its corresponding alcohol. Enzymes with glutarate semialdehyde reductase activity include the ATEG_00539 gene product of 95 Aspergillus terreus and 4-hydroxybutyrate dehydrogenase of Arabidopsis thaliana, encoded by 4hbd (WO 2010/068953A2). The A. thaliana enzyme was cloned and characterized in yeast (Breitkreuz et al., J.Biol.Chem. 278:41552-41556 (2003)). PROTEIN GENBANK ID GI NUMBER ORGANISM ATEG 00539 XP 001210625.1 115491995 Aspergillus terreus NIH2624 4hbd AAK94781.1 15375068 Arabidopsis thaliana 5 Additional genes encoding enzymes that catalyze the reduction of an aldehyde to alcohol (i.e., alcohol dehydrogenase or equivalently aldehyde reductase) include alrA encoding a medium-chain alcohol dehydrogenase for C2-C14 (Tani et al., AppL.Environ.Microbiol. 66:5231-5235 (2000)), yqhD andfAcO from E. coli (Sulzenbacher et al., 342:489-502 (2004)), and bdh I and bdh II from C. acetobutylicum which converts butyryaldehyde into 10 butanol (Walter et al., 174:7149-7158 (1992)). YqhD catalyzes the reduction of a wide range of aldehydes using NADPH as the cofactor, with a preference for chain lengths longer than C(3) (Sulzenbacher et al., 342:489-502 (2004);Perez et al., J Biol.Chem. 283:7346-7353 (2008)). The adhA gene product from Zymomonas mobilisE has been demonstrated to have activity on a number of aldehydes including formaldehyde, 15 acetaldehyde, propionaldehyde, butyraldehyde, and acrolein (Kinoshita et al., Appl Microbiol Biotechnol 22:249-254 (1985)). Additional aldehyde reductase candidates are encoded by bdh in C. saccharoperbutylacetonicum and Cbei_1722, Cbei_2181 and Cbei_2421 in C. Beijerinckii. Additional aldehyde reductase gene candidates in Saccharomyces cerevisiae include the aldehyde reductases GRE3, ALD2-6 and HFD 1, 20 glyoxylate reductases GORI and YPL1 13C and glycerol dehydrogenase GCY1 (WO 2011/022651A1; Atsumi et al., Nature 451:86-89 (2008)). The enzyme candidates described previously for catalyzing the reduction of methylglyoxal to acetol or lactaldehyde are also suitable lactaldehyde reductase enzyme candidates. Protein GENBANK ID GI NUMBER ORGANISM alrA BAB12273.1 9967138 Acinetobacter sp. strain M-1 ADH2 NP 014032.1 6323961 Saccharomyces cerevisiae yqhD NP 417484.1 16130909 Escherichia coli fucO NP 417279.1 16130706 Escherichia coli bdh I NP 349892.1 15896543 Clostridium acetobutylicum bdh II NP 349891.1 15896542 Clostridium acetobutylicum adhA YP 162971.1 56552132 Zymomonas mobilis bdh BAF45463.1 124221917 Clostridium saccharoperbutylacetonicum Cbei 1722 YP 001308850 150016596 Clostridium begerinckii Cbei 2181 YP 001309304 150017050 Clostridium beijerinckii 96 Protein GENBANK ID GI NUMBER ORGANISM Cbei 2421 YP 001309535 150017281 Clostridium beijerinckii GRE3 P38715.1 731691 Saccharomyces cerevisiae ALD2 CAA89806.1 825575 Saccharomyces cerevisiae ALD3 NP 013892.1 6323821 Saccharomyces cerevisiae ALD4 NP 015019.1 6324950 Saccharomyces cerevisiae ALD5 NP 010996.2 330443526 Saccharomyces cerevisiae ALD6 ABX39192.1 160415767 Saccharomyces cerevisiae HFD1 Q04458.1 2494079 Saccharomyces cerevisiae GOR I NP 014125.1 6324055 Saccharomyces cerevisiae YPLI13C AAB68248.1 1163100 Saccharomyces cerevisiae GCY1 CAA99318.1 1420317 Saccharomyces cerevisiae Enzymes exhibiting 4-hydroxybutyrate dehydrogenase activity (EC 1.1.1.61) also fall into this category. Such enzymes have been characterized in Ralstonia eutropha (Bravo et al., J Forens Sci, 49:379-387 (2004)) and Clostridium kluyveri (Wolff et al., Protein Expr.Purif 5 6:206-212 (1995)). Yet another gene is the alcohol dehydrogenase adhI from Geobacillus thermoglucosidasius (Jeon et al., JBiotechnol 135:127-133 (2008)). PROTEIN GENBANK ID GI NUMBER ORGANISM 4hbd YP 726053.1 113867564 Ralstonia eutropha H16 4hbd L21902.1 146348486 Clostridium kluyveri DSM 555 adhI AAR91477.1 40795502 Geobacillus thermoglucosidasius Another exemplary aldehyde reductase is methylmalonate semialdehyde reductase, also known as 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31). This enzyme participates in valine, leucine and isoleucine degradation and has been identified in bacteria, eukaryotes, 10 and mammals. The enzyme encoded by P84067 from Thernus thermophilus HB8 has been structurally characterized (Lokanath et al., JMolBiol, 352:905-17 (2005)). The reversibility of the human 3-hydroxyisobutyrate dehydrogenase was demonstrated using isotopically-labeled substrate (Manning et al., Biochem J, 231:481-4 (1985)). Additional genes encoding this enzyme include 3hidh in Homo sapiens (Hawes et al., Methods 15 Enzymol, 324:218-228 (2000)) and Oryctolagus cuniculus (Hawes et al., supra; Chowdhury et al., Biosci.Biotechnol Biochem. 60:2043-2047 (1996)), mmsB in Pseudomonas aeruginosa and Pseudomonas putida, and dhat in Pseudomonas putida (Aberhart et al., J Chen.Soc.[Perkin 1] 6:1404-1406 (1979); Chowdhury et al., Biosci.Biotechnol Biochem. 60:2043-2047 (1996); Chowdhury et al., Biosci.Biotechnol 20 Biochem. 67:438-441 (2003)). Several 3-hydroxyisobutyrate dehydrogenase enzymes have been characterized in the reductive direction, including mmsB from Pseudomonas 97 aeruginosa (Gokam et al., US Patent 739676, (2008)) and mmsB from Pseudomonas putida. PROTEIN GENBANK ID GI NUMBER ORGANISM P84067 P84067 75345323 Thermus thermophilus 3hidh P31937.2 12643395 Homo sapiens 3hidh P32185.1 416872 Oryctolagus cuniculus mmsB NP 746775.1 26991350 Pseudomonas putida mmsB P28811.1 127211 Pseudomonas aeruginosa dhat Q59477.1 2842618 Pseudomonas putida There exist several exemplary alcohol dehydrogenases that convert a ketone to a hydroxyl 5 functional group. Two such enzymes from E. coli are encoded by malate dehydrogenase (mdh) and lactate dehydrogenase (ldhA). In addition, lactate dehydrogenase from Ralstonia eutropha has been shown to demonstrate high activities on 2-ketoacids of various chain lengths includings lactate, 2-oxobutyrate, 2-oxopentanoate and 2 oxoglutarate (Steinbuchel et al., Eur.J.Biochem. 130:329-334 (1983)). Conversion of 10 alpha-ketoadipate into alpha-hydroxyadipate can be catalyzed by 2-ketoadipate reductase, an enzyme reported to be found in rat and in human placenta (Suda et al., Arch.Biochem.Biophys. 176:610-620 (1976); Suda et al., Biochem.Biophys.Res. Commun. 77:586-591 (1977)). An additional oxidoreductase is the mitochondrial 3-hydroxybutyrate dehydrogenase (bdh) from the human heart which has been cloned and characterized 15 (Marks et al., J.Biol.Chem. 267:15459-15463 (1992)). Alcohol dehydrogenase enzymes of C. beijerinckii (Ismaiel et al., J.Bacteriol. 175:5097-5105 (1993)) and T. brockii (Lamed et al., Biochem.J. 195:183-190 (1981); Peretz et al., Biochemistry. 28:6549-6555 (1989)) convert acetone to isopropanol. Methyl ethyl ketone reductase catalyzes the reduction of MEK to 2-butanol. Exemplary MEK reductase enzymes can be found in Rhodococcus 20 ruber (Kosjek et al., Biotechnol Bioeng. 86:55-62 (2004)) and Pyrococcusfuriosus (van der Oost et al., Eur.J.Biochem. 268:3062-3068 (2001)). Gene GenBank Accession No. GI No. Organism mdh AAC76268.1 1789632 Escherichia coli ldhA NP 415898.1 16129341 Escherichia coli ldh YP 725182.1 113866693 Ralstonia eutropha bdh AAA58352.1 177198 Homo sapiens adh AAA23199.2 60592974 Clostridium beijerinckii NRRL B593 adh P14941.1 113443 Thermoanaerobacter brockii HTD4 sadh CAD36475 21615553 Rhodococcus ruber adhA AAC25556 3288810 Pyrococcus furiosus 98 A number of organisms encode genes that catalyze the reduction of 3-oxobutanol to 1,3 butanediol, including those belonging to the genus Bacillus, Brevibacterium, Candida, and Klebsiella among others, as described by Matsuyama et al. JMol Cat B Enz, 11:513-521 (2001). One of these enzymes, SADH from Candida parapsilosis, was cloned and 5 characterized in E. coli. A mutated Rhodococcus phenylacetaldehyde reductase (Sar268) and a Leifonia alcohol dehydrogenase have also been shown to catalyze this transformation at high yields (Itoh et al., Appl.Microbiol Biotechnol. 75:1249-1256 (2007)). Gene GenBank Accession No. GI No. Organism sadh BAA24528.1 2815409 Candida parapsilosis 10 Alcohol dehydrogenase enzymes that reduce 3-oxoacyl-CoA substrates to their corresponding 3-hydroxyacyl-CoA product are also relevant to the pathways depicted in Figure 2 (step B) and Figure 3 (step B). Exemplary enzymes include 3-oxoacyl-CoA reductase and acetoacetyl-CoA reductase. 3-Oxoacyl-CoA reductase enzymes (EC 1.1.1.35) convert 3-oxoacyl-CoA molecules into 3-hydroxyacyl-CoA molecules and are 15 often involved in fatty acid beta-oxidation or phenylacetate catabolism. For example, subunits of two fatty acid oxidation complexes in E. coli, encoded byfadB andfadJ, function as 3-hydroxyacyl-CoA dehydrogenases (Binstock et al., Methods Enzymol. 71 Pt C:403-411 (1981)). Given the proximity in E. coli ofpaaH to other genes in the phenylacetate degradation operon (Nogales et al., 153:357-365 (2007)) and the fact that 20 paaH mutants cannot grow on phenylacetate (Ismail et al., Eur.JBiochem. 270:3047-3054 (2003)), it is expected that the E. coli paaH gene also encodes a 3-hydroxyacyl-CoA dehydrogenase. Additional 3-oxoacyl-CoA enzymes include the gene products ofphaC in Pseudomonas putida (Olivera et al., Proc.Natl.Acad.Sci U.S.A 95:6419-6424 (1998)) and paaC in Pseudomonasfluorescens (Di et al., 188:117-125 (2007)). These enzymes 25 catalyze the reversible oxidation of 3-hydroxyadipyl-CoA to 3-oxoadipyl-CoA during the catabolism of phenylacetate or styrene. Acetoacetyl-CoA reductase (EC 1.1.1.36) catalyzes the reduction of acetoacetyl-CoA to 3 hydroxybutyryl-CoA. This enzyme participates in the acetyl-CoA fermentation pathway to butyrate in several species of Clostridia and has been studied in detail (Jones et al., 30 Microbiol Rev. 50:484-524 (1986)). Acetoacetyl-CoA reducatse also participates in polyhydroxybutyrate biosynthesis in many organisms, and has also been used in metabolic engineering applications for overproducing PHB and 3-hydroxyisobutyrate (Liu et al., 99 AppL. Microbiol. Biotechnol. 76:811-818 (2007); Qui et al., Appl. Microbiol. Biotechnol. 69:537-542 (2006)). The enzyme from Clostridium acetobutylicum, encoded by hbd, has been cloned and functionally expressed in E. coli (Youngleson et al., JBacteriol. 171:6800-6807 (1989)). Additional gene candidates include phbB from Zoogloea 5 ramigera (Ploux et, al., Eur.JBiochem. 174:177-182 (1988)) and phaB from Rhodobacter sphaeroides (Alber et al., Mol.Microbiol 61:297-309 (2006)). The Z. ramigera gene is NADPH-dependent and the gene has been expressed in E. coli (Peoples et al., Mol.Microbiol 3:349-357 (1989)). Substrate specificity studies on the gene led to the conclusion that it could accept 3-oxopropionyl-CoA as a substrate besides acetoacetyl 10 CoA (Ploux et al., Eur.JBiochem. 174:177-182 (1988)). Additional genes include phaB in Paracoccus denitrificans, Hbd1 (C-terminal domain) and Hbd2 (N-terminal domain) in Clostridium kluyveri (Hillmer and Gottschalk, Biochim. Biophys. Acta 3334:12-23 (1974)) and HSD1 7B10 in Bos taurus (Wakil et al., JBiol.Chem. 207:631-638 (1954)). The enzyme from Paracoccus denitrificans has been functionally expressed and characterized 15 in E. coli (Yabutani et al., FEMS Microbiol Lett. 133:85-90 (1995)). A number of similar enzymes have been found in other species of Clostridia and in Metallosphaera sedula (Berg et al., Science. 318:1782-1786 (2007)). The enzyme from Candida tropicalis is a component of the peroxisomal fatty acid beta-oxidation multifunctional enzyme type 2 (MFE-2). The dehydrogenase B domain of this protein is catalytically active on 20 acetoacetyl-CoA. The domain has been functionally expressed in E. coli, a crystal structure is available, and the catalytic mechanism is well-understood (Ylianttila et al., Biochem Biophys Res Commun 324:25-30 (2004); Ylianttila et al., JMoI Biol 358:1286 1295 (2006)). Protein GENBANK ID GI NUMBER ORGANISM fadB P21177.2 119811 Escherichia coli fadJ P77399.1 3334437 Escherichia coli paaH NP 415913.1 16129356 Escherichia coli Hbd2 EDK34807.1 146348271 Clostridium kluyveri HbdJ EDK325 12.1 146345976 Clostridium kluyveri phaC NP 745425.1 26990000 Pseudomonas putida paaC ABF82235.1 106636095 Pseudomonas fluorescens HSD17B10 002691.3 3183024 Bos taurus phbB P23238.1 130017 Zoogloea ramigera phaB YP 353825.1 77464321 Rhodobacter sphaeroides phaB BAA08358 675524 Paracoccus denitrificans Hbd NP 349314.1 15895965 Clostridium acetobutylicum Hbd AAM 14586.1 20162442 Clostridium bejerinckii Msed 1423 YP 001191505 146304189 Metallosphaera sedula 100 Protein GENBANK ID GI NUMBER ORGANISM Msed 0399 YP 001190500 146303184 Metallosphaera sedula Msed 0389 YP 001190490 146303174 Metallosphaera sedula Msed 1993 YP 001192057 146304741 Metallosphaera sedula Fox2 Q02207 399508 Candida tropicalis 1.1.1.c Oxidoreductase (acyl-CoA to alcohol) Bifunctional oxidoreductases convert an acyl-CoA to its corresponding alcohol. Enzymes with this activity are required to convert 3-hydroxybutyryl-CoA to 1,3-butanediol (Step L 5 of Figure 5). Exemplary bifunctional oxidoreductases that convert an acyl-CoA to alcohol include those that transform substrates such as acetyl-CoA to ethanol (e.g., adhE from E. coli (Kessler et al., FEBS.Lett. 281:59-63 (1991))) and butyryl-CoA to butanol (e.g. adhE2 from C. acetobutylicum (Fontaine et al., J.Bacteriol, 184:821-830 (2002))). The C. acetobutylicum 10 enzymes encoded by bdh I and bdh II (Walter, et al., J. Bacteriol. 174:7149-7158 (1992)), reduce acetyl-CoA and butyryl-CoA to ethanol and butanol, respectively. In addition to reducing acetyl-CoA to ethanol, the enzyme encoded by adhE in Leuconostoc mesenteroides has been shown to oxide the branched chain compound isobutyraldehyde to isobutyryl-CoA (Kazahaya et al., J. Gen.Appl.Microbiol. 18:43-55 (1972); Koo et al., 15 Biotechnol Lett, 27:505-510 (2005)). Another exemplary enzyme can convert malonyl CoA to 3-HP. An NADPH-dependent enzyme with this activity has characterized in Chloroflexus aurantiacus where it participates in the 3-hydroxypropionate cycle (Hugler et al., JBacteriol, 184:2404-2410 (2002); Strauss et al., Eur JBiochem, 215:633-643 (1993)). This enzyme, with a mass of 300 kDa, is highly substrate-specific and shows little 20 sequence similarity to other known oxidoreductases (Hugler et al., supra). No enzymes in other organisms have been shown to catalyze this specific reaction; however there is bioinformatic evidence that other organisms may have similar pathways (Klatt et al., Env Microbiol, 9:2067-2078 (2007)). Enzyme candidates in other organisms including Roseiflexus castenholzii, Erythrobacter sp. NAP] and marine gamma proteobacterium 25 HTCC2080 can be inferred by sequence similarity. Protein GenBank ID Gi Number Organism adhE NP 415757.1 16129202 Escherichia coli adhE2 AAK09379.1 12958626 Clostridium acetobutylicum bdh I NP 349892.1 15896543 Clostridium acetobutylicum bdh II NP 349891.1 15896542 Clostridium acetobutylicum adhE AAV66076.1 55818563 Leuconostoc mesenteroides 101 Protein GenBank ID GI Number Or2ganism mcr AAS20429.1 42561982 Chloroflexus aurantiacus Rcas 2929 YP 001433009.1 156742880 Roseiflexus castenholzii NAP] 02720 ZP 01039179.1 85708113 Erythrobactersp. NAP] MGP2080_00535 ZP_01626393.1 119504313 marine gamma proteobacterium HTCC2080 Longer chain acyl-CoA molecules can be reduced to their corresponding alcohols by enzymes such as the jojoba (Simmondsia chinensis) FAR which encodes an alcohol forming fatty acyl-CoA reductase. Its overexpression in E. coli resulted in FAR activity 5 and the accumulation of fatty alcohol (Metz et al., Plant Physiol, 122:635-644 (2000)). Protein GenBank ID I GI Number I Organism FAR AAD38039.1 5020215 Simmondsia chinensis Another candidate for catalyzing these steps is 3-hydroxy-3-methylglutaryl-CoA reductase (or HMG-CoA reductase). This enzyme naturally reduces the CoA group in 3-hydroxy-3 methylglutaryl-CoA to an alcohol forming mevalonate. The hmgA gene of Sulfolobus 10 solfataricus, encoding 3-hydroxy-3-methylglutaryl-CoA reductase, has been cloned, sequenced, and expressed in E. coli (Bochar et al., JBacteriol. 179:3632-3638 (1997)). S. cerevisiae also has two HMG-CoA reductases in it (Basson et al., ProcNatl.Acad.Sci.U.S.A 83:5563-5567 (1986)). The gene has also been isolated from Arabidopsis thaliana and has been shown to complement the HMG-COA reductase 15 activity in S. cerevisiae (Learned et al., Proc.Natl.Acad.Sci.U.S.A 86:2779-2783 (1989)). Pro GenBank ID GI Oranism tein Number HMG] CAA86503.1 587536 Saccharomyces cerevisiae HMG2 NP 013555 6323483 Saccharomyces cerevisiae HMG] CAA70691.1 1694976 Arabidopsis thaliana hmgA AAC45370.1 2130564 Sulfolobus soffataricus 1.2.1.b Oxidoreductase (acVl-CoA to aldehyde) Acyl-CoA reductases in the 1.2.1 family reduce an acyl-CoA to its corresponding aldehyde. Such a conversion is required to catalyze the reduction of glutaryl-CoA to 20 glutarate semialdehyde (step S of Figure 1) and 3-hydroxybutyryl-CoA to 3 hydroxybutyraldehyde (step I of Figure 5). Several acyl-CoA reductase enzymes have 102 been described in the open literature and represent suitable candidates for this step. These are described below. Acyl-CoA reductases or acylating aldehyde dehydrogenases reduce an acyl-CoA to its corresponding aldehyde. Exemplary enzymes include fatty acyl-CoA reductase, succinyl 5 CoA reductase (EC 1.2.1.76), acetyl-CoA reductase, butyryl-CoA reductase and propionyl-CoA reductase (EC 1.2.1.3). Exemplary fatty acyl-CoA reductases enzymes are encoded by acrl of Acinetobacter calcoaceticus (Reiser, Journal ofBacteriology 179:2969-2975 (1997)) and Acinetobacter sp. M-1 (Ishige et al., Appl. Environ. Microbiol. 68:1192-1195 (2002)). Enzymes with succinyl-CoA reductase activity are encoded by 10 sucD of Clostridium kluyveri (Sohling, J. Bacteriol. 178:871-880 (1996)) and sucD of P. gingivalis (Takahashi, J. Bacteriol 182:4704-4710 (2000)). Additional succinyl-CoA reductase enzymes participate in the 3-hydroxypropionate/4-hydroxybutyrate cycle of thermophilic archaea including Metallosphaera sedula (Berg et al., Science 318:1782 1786 (2007)) and Thermoproteus neutrophilus (Ramos-Vera et al., J Bacteriol., 191:4286 15 4297 (2009)). The M. sedula enzyme, encoded by Msed_0709, is strictly NADPH dependent and also has malonyl-CoA reductase activity. The T. neutrophilus enzyme is active with both NADPH and NADH. The enzyme acylating acetaldehyde dehydrogenase in Pseudomonas sp, encoded by bphG, is yet another as it has been demonstrated to oxidize and acylate acetaldehyde, propionaldehyde, butyraldehyde, isobutyraldehyde and 20 formaldehyde (Powlowski, J. Bacteriol. 175:377-385 (1993)). In addition to reducing acetyl-CoA to ethanol, the enzyme encoded by adhE in Leuconostoc mesenteroides has been shown to oxidize the branched chain compound isobutyraldehyde to isobutyryl-CoA (Kazahaya, J. Gen. Appl, Microbiol. 18:43-55 (1972); and Koo et al., Biotechnol Lett. 27:505-510 (2005)). Butyraldehyde dehydrogenase catalyzes a similar reaction, 25 conversion of butyryl-CoA to butyraldehyde, in solventogenic organisms such as Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci Biotechnol Biochem., 71:58-68 (2007)). Exemplary propionyl-CoA reductase enzymes include pduP of Salmonella typhimurium LT2 (Leal, Arch. Microbiol. 180:353-361 (2003)) and eutE from E. coli (Skraly, WO Patent No. 2004/024876). The propionyl-CoA reductase of 30 Salmonella typhimurium LT2, which naturally converts propionyl-CoA to propionaldehyde, also catalyzes the reduction of 5-hydroxyvaleryl-CoA to 5 hydroxypentanal (WO 2010/068953A2).

103 Protein GenBank ID GI Number Organism acr1 YP 047869.1 50086359 Acinetobacter calcoaceticus acr] AAC45217 1684886 Acinetobacter baylyi acri BAB85476.1 18857901 Acinetobacter sp. Strain M-1 MSED 0709 YP 001190808.1 146303492 Metallosphaera sedula Tneu 0421 ACB39369.1 170934108 Thermoproteus neutrophilus sucD P38947.1 172046062 Clostridium kluyveri sucD NP 904963.1 34540484 Porphyromonas gingivalis bphG BAA03892.1 425213 Pseudomonas sp adhE AAV66076.1 55818563 Leuconostoc mesenteroides bld AAP42563.1 31075383 Clostridium saccharoperbutylacetonicum pduP NP 460996 16765381 Salmonella typhimurium LT2 eutE NP 416950 16130380 Escherichia coli An additional enzyme that converts an acyl-CoA to its corresponding aldehyde is malonyl CoA reductase which transforms malonyl-CoA to malonic semialdehyde. Malonyl-CoA reductase is a key enzyme in autotrophic carbon fixation via the 3-hydroxypropionate 5 cycle in thermoacidophilic archaeal bacteria (Berg, Science 318:1782-1786 (2007); and Thauer, Science 318:1732-1733 (2007)). The enzyme utilizes NADPH as a cofactor and has been characterized in Metallosphaera and Sulfolobus sp. (Alber et al., J. Bacteriol. 188:8551-8559 (2006); and Hugler, J. Bacteriol. 184:2404-2410 (2002)). The enzyme is encoded by Msed_0709 in Metallosphaera sedula (Alber et al., J. Bacteriol. 188:855 1 10 8559 (2006); and Berg, Science 318:1782-1786 (2007)). A gene encoding a malonyl-CoA reductase from Sulfolobus tokodaii was cloned and heterologously expressed in E. coli (Alber et al., J. Bacteriol 188:8551-8559 (2006). This enzyme has also been shown to catalyze the conversion of methylmalonyl-CoA to its corresponding aldehyde (W02007141208 (2007)). Although the aldehyde dehydrogenase functionality of these 15 enzymes is similar to the bifunctional dehydrogenase from Chloroflexus aurantiacus, there is little sequence similarity. Both malonyl-CoA reductase enzyme candidates have high sequence similarity to aspartate-semialdehyde dehydrogenase, an enzyme catalyzing the reduction and concurrent dephosphorylation of aspartyl-4-phosphate to aspartate semialdehyde. Additional gene candidates can be found by sequence homology to proteins 20 in other organisms including Sulfolobus solfataricus and Sulfolobus acidocaldarius and have been listed below. Yet another candidate for CoA-acylating aldehyde dehydrogenase is the ald gene from Clostridium beijerinckii (Toth, AppL. Environ. Microbiol. 65:4973 4980 (1999). This enzyme has been reported to reduce acetyl-CoA and butyryl-CoA to their corresponding aldehydes. This gene is very similar to eutE that encodes 104 acetaldehyde dehydrogenase of Salmonella typhimurium and E. coli (Toth, Appl. Environ. Microbiol. 65:4973-4980 (1999). Protein GenBank ID GI Number Organism Msed 0709 YP 001190808.1 146303492 Metallosphaera sedula Mcr NP 378167.1 15922498 Sulfolobus tokodali asd-2 NP 343563.1 15898958 Sulfolobus solfataricus Saci 2370 YP 256941.1 70608071 Sulfolobus acidocaldarius Ald AAT66436 49473535 Clostridium beijerinckii eutE AAA80209 687645 Salmonella typhimurium eutE P77445 2498347 Escherichia coli 1.2.1.c Oxidoreductase 2-oxoacid to acyl-CoA, decarboxylation 5 The reductive decarboxylation and acylation of 2-oxoadipate to glutarate semialdehyde (step D of Figure 1) is catalyzed by an oxidoreductase in EC class 1.2. A similar enzyme is required to convert 4-hydroxy-2-oxovalerate to 3-hydroxybutyryl-CoA (step H of Figure 5). Exemplary enzymes are found in the 2-ketoacid dehydrogenase and 2-ketoglutarate 10 ferredoxin oxidoreductase (OFOR) families. Alpha-ketoglutarate dehydrogenase (AKGD) converts alpha-ketoglutarate to succinyl CoA and is the primary site of control of metabolic flux through the TCA cycle (Hansford, Curr. Top.Bioenerg. 10:217-278 (1980)). Encoded by genes sucA, sucB and lpd in E. coli, AKGD gene expression is downregulated under anaerobic conditions and during growth 15 on glucose (Park et al., 15:473-482 (1995)). Although the substrate range of AKGD is narrow, structural studies of the catalytic core of the E2 component pinpoint specific residues responsible for substrate specificity (Knapp et al., JMol.Biol. 280:655-668 (1998)). The Bacillus subtilis AKGD, encoded by odhAB (El and E2) and pdhD (E3, shared domain), is regulated at the transcriptional level and is dependent on the carbon 20 source and growth phase of the organism (Resnekov et al., Mol.Gen.Genet. 234:285-296 (1992)). In yeast, the LPD1 gene encoding the E3 component is regulated at the transcriptional level by glucose (Roy et al., J.Gen.Microbiol. 133:925-933 (1987)). The El component, encoded by KGD1, is also regulated by glucose and activated by the products of HAP2 and HAP3 (Repetto et al., Mol.Cell Biol. 9:2695-2705 (1989)). The AKGD 25 enzyme complex, inhibited by products NADH and succinyl-CoA, is well-studied in mammalian systems, as impaired function of has been linked to several neurological diseases.

105 Gene GI # Accession No. Organism sucA 16128701 NP 415254.1 Escherichia coli sucB 16128702 NP 415255.1 Escherichia coli lpd 16128109 NP 414658.1 Escherichia coli odhA 51704265 P23129.2 Bacillus subtilis odhB 129041 P 16263.1 Bacillus subtilis pdhD 118672 P21880.1 Bacillus subtilis KGD1 6322066 NP 012141.1 Saccharomyces cerevisiae KGD2 6320352 NP 010432.1 Saccharomyces cerevisiae LPD 1 14318501 NP 116635.1 Saccharomyces cerevisiae Branched-chain 2-keto-acid dehydrogenase complex (BCKAD), also known as 2 oxoisovalerate dehydrogenase, participates in branched-chain amino acid degradation pathways, converting 2-keto acids derivatives of valine, leucine and isoleucine to their 5 acyl-CoA derivatives and CO 2 . The complex has been studied in many organisms including Bacillus subtilis (Wang et al., Eur.J.Biochem. 213:1091-1099 (1993)), Rattus norvegicus (Namba et al., J.Biol.Chem. 244:4437-4447 (1969)) and Pseudomonas putida (Sokatch et al., 148:647-652 (1981)). In Bacillus subtilis the enzyme is encoded by genes pdhD (E3 component), bfmBB (E2 component), bfinBAA and bfinBAB (El component) 10 (Wang et al., Eur.J.Biochem. 213:1091-1099 (1993)). In mammals, the complex is regulated by phosphorylation by specific phosphatases and protein kinases. The complex has been studied in rat hepatocites (Chicco et al., J.Biol.Chem. 269:19427-19434 (1994)) and is encoded by genes Bckdha (El alpha), Bckdhb (El beta), Dbt (E2), and Dld (E3). The El and E3 components of the Pseudomonas putida BCKAD complex have been 15 crystallized (Aevarsson et al., Nat.Struct.Biol. 6:785-792 (1999); Mattevi et al., Science. 255:1544-1550 (1992)) and the enzyme complex has been studied (Sokatch et al., 148:647-652 (1981)). Transcription of the P. putida BCKAD genes is activated by the gene product of bkdR (Hester et al., 233:828-836 (1995)). In some organisms including Rattus norvegicus (Paxton et al., Biochem.J. 234:295-303 (1986)) and Saccharomyces 20 cerevisiae (Sinclair et al., Biochem.Mol.Biol.Int. 31:911-922 (1993)), this complex has been shown to have a broad substrate range that includes linear oxo-acids such as 2 oxobutanoate and alpha-ketoglutarate, in addition to the branched-chain amino acid precursors. The active site of the bovine BCKAD was engineered to favor alternate substrate acetyl-CoA (Meng et al., Biochemistry. 33:12879-12885 (1994)). Gene Accession No. GI # Organism bfmBB NP 390283.1 16079459 Bacillus subtilis bfmBAA NP 390285.1 16079461 Bacillus subtilis bfmBAB NP 390284.1 16079460 Bacillus subtilis 106 Gene Accession No. .GI # Organism pdhD P21880.1 118672 Bacillus subtilis SlpdV P09063.1 118677 Pseudomonas putida bkdB P09062.1 129044 Pseudomonas putida bkdAl NP 746515.1 26991090 Pseudomonas putida bkdA2 NP 746516.1 26991091 Pseudomonas putida Bckdha NP 036914.1 77736548 Rattus norvegicus Bckdhb NP 062140.1 158749538 Rattus norvegicus Dbt NP 445764.1 158749632 Rattus norvegicus Did NP 955417.1 40786469 Rattus norvegicus The pyruvate dehydrogenase complex, catalyzing the conversion of pyruvate to acetyl CoA, has also been extensively studied. In the E. coli enzyme, specific residues in the El component are responsible for substrate specificity (Bisswanger, 256:815-822 (1981); 5 Bremer, 8:535-540 (1969); Gong et al., 275:13645-13653 (2000)). As mentioned previously, enzyme engineering efforts have improved the E. coli PDH enzyme activity under anaerobic conditions (Kim et al., J.BacterioL. 190:3851-3858 (2008); Kim et al., Appl.Environ.Microbiol. 73:1766-1771 (2007); Zhou et al., BiotechnoL.Lett. 30:335-342 (2008)). In contrast to the E. coli PDH, the B. subtilis complex is active and required for 10 growth under anaerobic conditions (Nakano et al., 179:6749-6755 (1997)). The Klebsiella pneumoniae PDH, characterized during growth on glycerol, is also active under anaerobic conditions (Menzel et al., 56:135-142 (1997)). Crystal structures of the enzyme complex from bovine kidney (Zhou et al., 98:14802-14807 (2001)) and the E2 catalytic domain from Azotobacter vinelandii are available (Mattevi et al., Science. 255:1544-1550 (1992)). 15 Some mammalian PDH enzymes complexes can react on alternate substrates such as 2 oxobutanoate. Comparative kinetics of Rattus norvegicus PDH and BCKAD indicate that BCKAD has higher activity on 2-oxobutanoate as a substrate (Paxton et al., Biochem.J. 234:295-303 (1986)). The S. cerevisiae complex consists of an E2 (LATI) core that binds El (PDA1, PDB1), E3 (LPD1), and Protein X (PDX1) components (Pronk et al., Yeast 20 12:1607-1633 (1996)). Gene Accession No. GI # Organism aceE NP 414656.1 16128107 Escherichia coli aceF NP 414657.1 16128108 Escherichia coli lpd NP 414658.1 16128109 Escherichia coli pdhA P21881.1 3123238 Bacillus subtilis pdhB P21882.1 129068 Bacillus subtilis pdhC P21883.2 129054 Bacillus subtilis pdhD P21880.1 118672 Bacillus subtilis aceE YP 001333808.1 152968699 Klebsiella pneumonia aceF YP 001333809.1 152968700 Klebsiella pneumonia 107 Gene Accession No. GI # Organism lpdA YP 001333810.1 152968701 Klebsiella pneumonia Pdhal NP 001004072.2 124430510 Rattus norvegicus Pdha2 NP 446446.1 16758900 Rattus norvegicus Dat NP 112287.1 78365255 Rattus norvegicus Did NP 955417.1 40786469 Rattus norvegicus LAT1 NP 014328 6324258 Saccharomyces cerevisiae PDA] NP 011105 37362644 Saccharomyces cerevisiae PDB1 NP 009780 6319698 Saccharomyces cerevisiae LPD1 NP 116635 14318501 Saccharomyces cerevisiae PDX1 NP 011709 6321632 Saccharomyces cerevisiae As an alternative to the large multienzyme 2-keto-acid dehydrogenase complexes described above, some anaerobic organisms utilize enzymes in the 2-ketoacid oxidoreductase family (OFOR) to catalyze acylating oxidative decarboxylation of 2-keto 5 acids. Unlike the dehydrogenase complexes, these enzymes contain iron-sulfur clusters, utilize different cofactors, and use ferredoxin, flavodixin or FAD as electron donors in lieu of NAD(P)H. While most enzymes in this family are specific to pyruvate as a substrate (POR) some 2-keto-acid:ferredoxin oxidoreductases have been shown to accept a broad range of 2-ketoacids as substrates including alpha-ketoglutarate and 2-oxobutanoate 10 (Zhang et al., J.Biochem. 120:587-599 (1996); Fukuda et al., Biochim.Biophys.Acta 1597:74-80 (2002)). One such enzyme is the OFOR from the thermoacidophilic archaeon Sulfolobus tokodaii 7, which contains an alpha and beta subunit encoded by gene ST2300 (Zhang et al., J.Biochem. 120:587-599 (1996); Fukuda and Wakagi, Biochim.Biophys.Acta 1597:74-80 (2002)). A plasmid-based expression system has been 15 developed for efficiently expressing this protein in E. coli (Fukuda et al., Eur.J.Biochem. 268:5639-5646 (2001)) and residues involved in substrate specificity were determined (Fukuda and Wakagi, Biochim.Biophys.Acta 1597:74-80 (2002)). The 2 oxoacid:ferredoxin oxidoreductase from Sulfolobus solfataricus P1 is also active on a broad range of 2-oxoacids (Park et al., J.Biochem.Mol.Biol. 39:46-54 (2006)). The OFOR 20 enzyme encoded by Ape1472/Ape1473 from Aeropyrum pernix str. KI was recently cloned into E. coli, characterized, and found to react with 2-oxoglutarate and a broad range of 2-oxoacids (Nishizawa et al., FEBS Lett. 579:2319-2322 (2005)). There is bioinformatic evidence that similar enzymes are present in all archaea, some anaerobic bacteria and amitochondrial eukarya (Fukuda and Wakagi, supra). OFOR enzymes are 25 also found in organisms that fix carbon by the RTCA cycle including Hydrogenobacter thermophilus, Desulfobacter hydrogenophilus and Chlorobium species (Shiba et al., Archives of Microbiology 141:198-203 (1985); Evans et al., Proc.Natl.Acad.Sci.U.S.A 108 55:928-934 (1966)). The two-subunit enzyme from H. thermophilus, encoded by korAB, was cloned and expressed in E. coli (Yun et al., Biochem.Biophys.Res.Commun. 282:589 594 (2001)). Gene GI # Accession No. Organism ST2300 NP 378302.1 15922633 Sulfolobus tokodaii 7 A pe1472 BAA80470.1 5105156 Aeropyrum pernix A pe1473 BAA8047 1.2 116062794 Aeropyrum pernix korA BAB21494 12583691 Hydrogenobacter thermophilus korB BAB21495 12583692 Hydrogenobacterthermophilus 5 1.2.1.d Oxidoreductase (dephosphorylatilg) The reduction of a phosphonic acid to its corresponding aldehyde is catalyzed by an oxidoreductase or phosphate reductase in the EC class 1.2.1. Step E of Figure 6 requires such an enzyme for the reduction of 2,4-pentadienoyl-phosphate to its corresponding 10 aldehyde. This transformation has not been characterized in the literature to date. Exemplary phosphonate reductase enzymes include glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), aspartate-semialdehyde dehydrogenase (EC 1.2.1.11) acetylglutamylphosphate reductase (EC 1.2.1.38) and glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.-). Aspartate semialdehyde dehydrogenase (ASD, EC 1.2.1.11) 15 catalyzes the NADPH-dependent reduction of 4-aspartyl phosphate to aspartate-4 semialdehyde. ASD participates in amino acid biosynthesis and recently has been studied as an antimicrobial target (Hadfield et al., Biochemistry 40:14475-14483 (2001)). The E. coli ASD structure has been solved (Hadfield et al., JMol.Biol. 289:991-1002 (1999)) and the enzyme has been shown to accept the alternate substrate beta-3-methylaspartyl 20 phosphate (Shames et al., JBiol.Chem. 259:15331-15339 (1984)). The Haemophilus influenzae enzyme has been the subject of enzyme engineering studies to alter substrate binding affinities at the active site (Blanco et al., Acta Crystallogr.D.Biol.Crystallogr. 60:1388-1395 (2004); Blanco et al., Acta Crystallogr.D.Biol.Crystallogr. 60:1808-1815 (2004)). Other ASD candidates are found in Mycobacterium tuberculosis (Shafiani et al., J 25 ApplMicrobiol 98:832-838 (2005)), Methanococcusjannaschii (Faehnle et al., JMol.Biol. 353:1055-1068 (2005)), and the infectious microorganisms Vibrio cholera and Heliobacterpylori (Moore et al., Protein Expr.Purif 25:189-194 (2002)). A related enzyme candidate is acetylglutamylphosphate reductase (EC 1.2.1.38), an enzyme that naturally reduces acetylglutamylphosphate to acetylglutamate-5-semialdehyde, found in S. 30 cerevisiae (Pauwels et al., Eur.JBiochem. 270:1014-1024 (2003)), B. subtilis (O'Reilly et 109 al., Microbiology 140 ( Pt 5):1023-1025 (1994)), E. coli (Parsot et al., Gene. 68:275-283 (1988)), and other organisms. Additional phosphate reductase enzymes of E. coli include glyceraldehyde 3-phosphate dehydrogenase (gapA (Branlant et al., Eur.J.Biochem. 150:61-66 (1985))) and glutamate-5-semialdehyde dehydrogenase (proA (Smith et al., 5 J.Bacteriol. 157:545-551 (1984))). Genes encoding glutamate-5-semialdehyde dehydrogenase enzymes from Salmonella typhimurium (Mahan et al., JBacteriol. 156:1249-1262 (1983)) and Campylobacterjejuni (Louie et al, Mol.Gen.Genet. 240:29-35 (1993)) were cloned and expressed in E. coli. Protein GenBanklD GI Number Or2anism asd NP 417891.1 16131307 Escherichia coli asd YP 248335.1 68249223 Haemophilus influenzae asd AAB49996 1899206 Mycobacterium tuberculosis VC2036 NP 231670 15642038 Vibrio cholera asd YP 002301787.1 210135348 Heliobacter pylori ARG5,6 NP 010992.1 6320913 Saccharomyces cerevisiae argC NP 389001.1 16078184 Bacillus subtilis argC NP 418393.1 16131796 Escherichia coli gapA POA9B2.2 71159358 Escherichia coli proA NP 414778.1 16128229 Escherichia coli proA NP 459319.1 16763704 Salmonella typhimurium proA P53000.2 9087222 Campylobacterjejuni 10 1.2.1.e Oxidoreductase (acid to aldehyde) The conversion of an acid to an aldehyde is thermodynamically unfavorable and typically requires energy-rich cofactors and multiple enzymatic steps. Direct conversion of the acid to aldehyde by a single enzyme is catalyzed by an acid reductase enzyme in the 1.2.1 15 family. An enzyme in this EC class is required to convert 3-hydroxybutyrate to 3 hydroxybutanal (Step 5K of Figure 5) and 2,4-pentadienoate to penta-2,4-dienal (Step A of Figure 6). Exemplary acid reductase enzymes include carboxylic acid reductase, alpha-aminoadipate reductase and retinoic acid reductase. Carboxylic acid reductase (CAR), found in 20 Nocardia iowensis, catalyzes the magnesium, ATP and NADPH-dependent reduction of carboxylic acids to their corresponding aldehydes (Venkitasubramanian et al., J Biol. Chem. 282:478-485 (2007)). The natural substrate of this enzyme is benzoate and the enzyme exhibits broad acceptance of aromatic substrates including p-toluate (Venkitasubramanian et al., Biocatalysis in Pharmaceutical and Biotechnology Industries.

110 CRC press (2006)). The enzyme from Nocardia iowensis, encoded by car, was cloned and functionally expressed in E. coli (Venkitasubramanian et al., JBio. Chem. 282:478-485 (2007)). CAR requires post-translational activation by a phosphopantetheine transferase (PPTase) that converts the inactive apo-enzyme to the active holo-enzyme (Hansen et al., 5 AppLEnviron.Microbiol 75:2765-2774 (2009)). Expression of the npt gene, encoding a specific PPTase, product improved activity of the enzyme. An additional enzyme candidate found in Streptomyces griseus is encoded by the griC and griD genes. This enzyme is believed to convert 3-amino-4-hydroxybenzoic acid to 3-amino-4 hydroxybenzaldehyde as deletion of either griC or griD led to accumulation of 10 extracellular 3-acetylamino-4-hydroxybenzoic acid, a shunt product of 3-amino-4 hydroxybenzoic acid metabolism (Suzuki, et al., J. Antibiot. 60(6):380-387 (2007)). Co expression of griC and griD with SGR_665, an enzyme similar in sequence to the Nocardia iowensis npt, can be beneficial. Gene GenBank Accession No. GI No. Organism car AAR91681.1 40796035 Nocardia iowensis npt AB183656.1 114848891 Nocardia iowensis griC YP 001825755.1 182438036 Streptomyces gniseus griD YP 001825756.1 182438037 Streptomyces griseus 15 Additional car and npt genes can be identified based on sequence homology. Gene name GI No. GenBank Accession No. Organism fadD9 121638475 YP_978699.1 Mycobacterium bovis BCG BCG_2812c 121638674 YP_978898.1 Mycobacterium bovis _ _ BCG nfa20150 54023983 YP_ 118225.1 ardiafarcinica IFM nfa40540 54026024 YP_120266.1 Nocardiafarcinica IFM YP_001828302. Streptomyces griseus SGR_6790 182440583 1 subsp. griseus NBRC 13350 YP_001822177. Streptomyces griseus SGR_665 182434458 1 subsp. griseus NBRC 13350 MSMEG_2956 YP_887275.1 YP_887275.1 Mycobacterium smegmatis MC2 155 MSMEG_5739 YP_889972.1 118469671 Mycobacterium smegmatis MC2 155 MSMEG_2648 YP_886985.1 118471293 Mycobacterium smegmatis MC2 155 111 Gene name GI No. A nNo Organism Mycobacterium avium MAP1040c NP_959974.1 41407138 subsp. paratuberculosis K-10 Mycobacterium avium MAP2899c NP_961833.1 41408997 subsp. paratuberculosis K-10 MMAR2117 YP001850422. 183982131 Mycobacterium marinum 1 M MMAR_2936 YP_001851230. 183982939 Mycobacterium marinum MMAR_1916 YP001850220. 183981929 Mycobacterium marinum TpauDR AFT_33 Tsukamurella 060 ZP04027864.1 227980601 paurometabola DSM 20162 Tsukamurella TpauDRAFT_20 ZP_04026660.1 ZP_04026660.1 paurometabola DSM 920 20162 CPCC7001132 ZP_05045132.1 254431429 Cyanobium PCC7001 DDBDRAFT_01 XP63693 1 66806417 Dictyostelium discoideum 87729 AX4 An enzyme with similar characteristics, alpha-aminoadipate reductase (AAR, EC 1.2.1.31), participates in lysine biosynthesis pathways in some fungal species. This enzyme naturally reduces alpha-aminoadipate to alpha-aminoadipate semialdehyde. The 5 carboxyl group is first activated through the ATP-dependent formation of an adenylate that is then reduced by NAD(P)H to yield the aldehyde and AMP. Like CAR, this enzyme utilizes magnesium and requires activation by a PPTase. Enzyme candidates for AAR and its corresponding PPTase are found in Saccharomyces cerevisiae (Morris et al., Gene 98:141-145 (1991)), Candida albicans (Guo et al., Mol.Genet.Genomics 269:271-279 10 (2003)), and Schizosaccharomyces pombe (Ford et al., Curr.Genet. 28:131-137 (1995)). The AAR from S. pombe exhibited significant activity when expressed in E. coli (Guo et al., Yeast 21:1279-1288 (2004)). The AAR from Penicillium chrysogenum accepts S carboxymethyl-L-cysteine as an alternate substrate, but did not react with adipate, L glutamate or diaminopimelate (Hijarrubia et al., JBiol.Chem. 278:8250-8256 (2003)). The 15 gene encoding the P. chrysogenum PPTase has not been identified to date and no high confidence hits were identified by sequence comparison homology searching. Gene GenBank Accession No. I GI No. Organism LYS2 AAA34747.1 171867 Saccharomyces cerevisiae 112 Gene GenBank Accession No. GI No. Organism LYS5 P50113.1 1708896 Saccharomyces cerevisiae LYS2 AAC02241.1 2853226 Candida albicans LYS5 AA026020.1 28136195 Candida albicans Lyslp P40976.3 13124791 Schizosaccharomyces pombe Lys7p Q10474.1 1723561 Schizosaccharomyces pombe Lys2 CAA74300.1 3282044 Penicillium chrysogenum 1.3.1.a Oxidoreducatse (alkane to alkene) Several transformations in Figure 1 involve the oxidation of an alkane to an alkene, 5 including steps B, F and M. Steps B and F are catalyzed by a dehydrogenase or enoate reductase operating in the reverse direction. Step M is catalyzed by 5-hydroxyvaleryl-CoA dehydrogenase, an acyl-CoA dehydrogenase or enoate reductase. Steps E and G of Figure 3 entail oxidation of pent-3-enoyl-CoA or pent-2-enoyl-CoA, respectively, to 2,4 pentadienoyl-CoA. Exemplary enzyme candidates are described below. 10 The oxidation of pent-3-enoyl-CoA or pent-2-enoyl-CoA to 2,4-pentadienoyl-CoA is catalyzed by 2,4-pentadienoyl-CoA forming dehydrogenase enzymes. 2,4-Dienoyl-CoA reductase enzymes (EC 1.3.1.34) are suitable candidates for these transformations. Generally, bacterial 2,4-dienoyl-CoA reductases yield 2-enoyl-CoA products, whereas eukaryotic 2,4-dienoyl-CoA reductases yield 3-enoyl-CoA products (Dommes and Kunau, 15 JBiol Chem, 259:1781-1788 (1984)). ThefadH gene product of E. coli is an NADPH dependent 2,4-dienoyl-CoA reductase, which participates in the beta-oxidation of unsaturated fatty acids (Tu et al, Biochem, 47:1167-1175 (2008). A series of mutant DCR enzymes were constructed and shown to yield both 2-enoyl-CoA and 3-enoyl-CoA products (Tu et al, supra). Eukaryotic DCR enzymes have been characterized in humans 20 and the mouse (Koivuranta et al, Biochem J, 304:787-792 (1994); Geisbrecht et al, J Biol Chem 274:25814-20 (1999); Miinalainen et al, PLoS genet. 5: E1000543 (2009)). The 2,4 pentadienoyl-CoA reductase of Clostridium aminovalericum was shown to catalyze the oxidation of 3-pent-3-enoyl-CoA to 2,4-pentadienoyl-CoA. This enzyme has been purified, characterized and crystallized (Eikmanns, Acta Cryst, D50: 913-914 (1994) and 25 Eikmanns and Buckel, Eur JBiochem 198:263-266 (1991)). The electron carrier of this enzyme is not known; however, it is not NAD(P)H. The sequence of the enzyme has not been published to date.

113 Protein GenBank ID GI Number Organism fadH NP_417552.1 16130976 Escherichia coli Decr1 Q16698.1 3913456 Homo sapiens Pdcr Q9WV68.1 90109767 Mus musculus Decr NP_080448.1 13385680 Mus musculus 2-Enoate reductase enzymes in the EC classes 1.3.* are known to catalyze the reversible reduction of a wide variety of a, p-unsaturated carboxylic acids and aldehydes (Rohdich et al., JBiol Chem 276:5779-5787 (2001)). In the recently published genome sequence of C. 5 kluyveri, 9 coding sequences for enoate reductases were reported, out of which one has been characterized (Seedorf et al., PNAS 105:2128-2133 (2008)). The enr genes from both C. tyrobutyricum and Moorella thermoaceticum have been cloned and sequenced and show 59% identity to each other. The former gene is also found to have approximately 75% similarity to the characterized gene in C. kluyveri (Giesel et al., 135:51-57 (1983)). It 10 has been reported based on these sequence results that the C. tyrobutyricum enr is very similar to the FadH dienoyl CoA reductase of E. coli (Rohdich et al., supra). The M. thermoaceticum enr gene was expressed in a catalytically active form in E. coli (Rohdich et al., supra). This enzyme exhibits activity on a broad range of alpha, beta-unsaturated carbonyl compounds. Protein GenBank ID GI Number Organism enr ACA54153.1 169405742 Clostridium botulinum A3 str enr CAA71086.1 2765041 Clostridium tyrobutyricum enr CAA76083.1 3402834 Clostridium kluyveri enr YP_430895.1 83590886 Moorella thermoacetica 15 Another candidate 2-enoate reductase is maleylacetate reductase (MAR, EC 1.3.1.32), an enzyme catalyzing the reduction of 2-maleylacetate (4-oxohex-2-enedioate) to 3 oxoadipate. MAR enzymes naturally participate in aromatic degradation pathways (Kaschabek et al., JBacteriol. 175:6075-6081 (1993); Kaschabek et al., JBacteriol. 20 177:320-325 (1995); Camara et al., JBacteriol. (2009); Huang et al., Appl Environ.Microbiol 72:7238-7245 (2006)). The enzyme activity was identified and characterized in Pseudomonas sp. strain B13 (Kaschabek et al., 175:6075-6081 (1993); Kaschabek et al., 177:320-325 (1995)), and the coding gene was cloned and sequenced (Kasberg et al., JBacteriol. 179:3801-3803 (1997)). Additional MAR gene candidates 25 include cicE gene from Pseudomonas sp. strain B13 (Kasberg et al., JBacteriol.

114 179:3801-3803 (1997)), macA gene from Rhodococcus opacus (Seibert et al., 180:3503 3508 (1998)), the macA gene from Ralstonia eutropha (also known as Cupriavidus necator) (Seibert et al., Microbiology 150:463-472 (2004)), tfdFII from Ralstonia eutropha (Seibert et al., JBacteriol. 175:6745-6754 (1993)) and NCgl1112 in 5 Corynebacterium glutamicum (Huang et al., Appl Environ.Microbiol 72:7238-7245 (2006)). A MAR in Pseudomonas reinekei MT, encoded by ccaD, was recently identified (Camara et al., JBacteriol. (2009)). Gene GI # Accession No. Organism clcE 3913241 030847.1 Pseudomonas sp. strain B13 macA 7387876 084992.1 Rhodococcus opacus macA 5916089 AAD55886 Cupriavidus necator tfdFII 1747424 AC44727.1 Ralstonia eutropha JMP134 NCglI 112 19552383 NP_600385 Corynebacterium glutamicum ccaD AB061029.1 134133940 Pseudomonas reinekei MT1 An exemplary enoate reductase that favors the alkene-forming oxidative direction is 10 succinate dehydrogenase (EC classes 1.3.99 or 1.3.5), also known as succinate-ubiquinone oxidoreductase and complex II. SDH is a membrane-bound enzyme complex that converts succinate to fumarate and transfers electrons to ubiquinone. The enzyme is composed of two catalytic subunits, encoded by sdhAB, and two membrane subunits encoded by sdhCD. Although the E. coli SDH is reversible, the enzyme is 50-fold more proficient in 15 oxidizing succinate than reducing fumarate (Maklashina et al J Biol.Chem. 281:11357 11365 (2006)). Protein GenBank ID GI Number Organism sdhA AAC73817.1 1786942 Escherichia coli sdhB AAC73818.1 1786943 Escherichia coli sdhC AAC73815.1 1786940 Escherichia coli sdhD AAC73816.1 1786941 Escherichia coli An exemplary acyl-CoA dehydrogenase or enoyl-CoA reductase is the gene product of bcd from Clostridium acetobutylicum (Atsumi et al., 10:305-311 (2008); Boynton et al., J 20 Bacteriol. 178:3015-3024 (1996)), which naturally catalyzes the reduction of crotonyl CoA to butyryl-CoA (EC 1.3.99.2). This enzyme participates in the acetyl-CoA fermentation pathway to butyrate in Clostridial species (Jones et al., Microbiol Rev. 50:484-524 (1986)). Activity of butyryl-CoA reductase can be enhanced by expressing 115 bcd in conjunction with expression of the C. acetobutylicum etfAB genes, which encode an electron transfer flavoprotein. An additional candidate for the enoyl-CoA reductase step is the mitochondrial enoyl-CoA reductase (EC 1.3.1.44) from E. gracilis (Hoffimeister et al., JBiol.Chem 280:4329-4338 (2005)). A construct derived from this sequence following the 5 removal of its mitochondrial targeting leader sequence was cloned in E. coli resulting in an active enzyme (Hoffmeister et al, supra). A close homolog of the protein from the prokaryote Treponema denticola, encoded by TDE0597, has also been cloned and expressed in E. coli (Tucci et al., FEBS Lett, 581:1561-1566 (2007)). Six genes in Syntrophus aciditrophicus were identified by sequence homology to the C. acetobutylicum 10 bcd gene product. The S. aciditrophicus genes syn_02637 and syn_02636 bear high sequence homology to the etfAB genes of C. acetobutylicum, and are predicted to encode the alpha and beta subunits of an electron transfer flavoprotein. Protein GenBank ID GI Number Organism bcd NP_349317.1 15895968 Clostridium acetobutylicum etfA NP_349315.1 15895966 Clostridium acetobutylicum etfB NP_349316.1 15895967 Clostridium acetobutylicum TER Q5EU90.1 62287512 Euglena gracilis TDE0597 NP_971211.1 42526113 Treponema denticola syn_02587 ABC76101 85721158 Syntrophus aciditrophicus syn_02586 ABC76100 85721157 Syntrophus aciditrophicus syn 01146 ABC76260 85721317 Syntrophus aciditrophicus syn_00480 ABC77899 85722956 Syntrophus aciditrophicus syn_02128 ABC76949 85722006 Syntrophus aciditrophicus syn_01699 ABC78863 85723920 Syntrophus aciditrophicus syn_02637 ABC78522.1 85723579 Syntrophus aciditrophicus syn_02636 ABC78523.1 85723580 Syntrophus aciditrophicus Additional enoyl-CoA reductase enzyme candidates are found in organisms that degrade 15 aromatic compounds. Rhodopseudomonas palustris, a model organism for benzoate degradation, has the enzymatic capability to degrade pimelate via beta-oxidation of pimeloyl-CoA. Adjacent genes in the pim operon, pimC and pimD, bear sequence homology to C. acetobutylicum bcd and are predicted to encode a flavin-containing pimeloyl-CoA dehydrogenase (Harrison et al., 151:727-736 (2005)). The genome of 20 nitrogen-fixing soybean symbiont Bradyrhizobiumjaponicum also contains apim operon composed of genes with high sequence similarity to pimC and pimD of R. palustris (Harrison and Harwood, Microbiology 151:727-736 (2005)).

116 Protein GenBank ID GI Number Organism pimC CAE29155 39650632 Rhodopseudomonas palustris pimD CAE29154 39650631 Rhodopseudomonas palustris pimC BAC53083 27356102 Bradyrhizobiumjaponicum pimD BAC53082 27356101 Bradyrhizobiumjaponicum An additional candidate is 2-methyl-branched chain enoyl-CoA reductase (EC 1.3.1.52 and EC 1.3.99.12), an enzyme catalyzing the reduction of sterically hindered trans-enoyl CoA substrates. This enzyme participates in branched-chain fatty acid synthesis in the 5 nematode Ascarius suum and is capable of reducing a variety of linear and branched chain substrates including 2-methylvaleryl-CoA, 2-methylbutanoyl-CoA, 2-methylpentanoyl CoA, octanoyl-CoA and pentanoyl-CoA (Duran et al., 268:22391-22396 (1993)). Two isoforms of the enzyme, encoded by genes acad1 and acad, have been characterized. Protein GenBank ID GI Number Organism acadi AAC48316.1 2407655 Ascarius suum acad AAA16096.1 347404 Ascarius suum 10 14.1.a Oxidoreductase (amine to oxo) Enzymes in the EC class 1.4.1 catalyze the oxidative deamination of amines to aldehydes or ketones. Enzymes in this EC class typically employ NAD+, NADP+ or FAD as an electron acceptor, and the reactions are typically reversible. Steps C, H and I of Figure 1 15 can be catalyzed by a deaminating oxidoreductase. Enzyme candidates are described below. Glutamate dehydrogenase (EC 1.4.1.2), leucine dehydrogenase (EC 1.4.1.9), and aspartate dehydrogenase (EC 1.4.1.2 1) convert amino acids to their corresponding 2-keto acids. The gdhA gene product from Escherichia coli (Korber et al., JMol.Biol. 234:1270-1273 20 (1993); McPherson et al., Nucleic Acids Res. 11:5257-5266 (1983)), gdh from Thermotoga maritime (Kort et al., Extremophiles. 1:52-60 (1997); Lebbink et al., JMol.Biol. 280:287 296 (1998); Lebbink et al., JMol.Biol. 289:357-369 (1999)), and gdhA1 from Halobacterium salinarum (Ingoldsby et al., Gene 349:237-244 (2005)) catalyze the reversible conversion of glutamate to 2-oxoglutarate and ammonia, while favoring 25 NADP(H), NAD(H), or both, respectively. Additional glutamate dehydrogenase gene candidates are found in Bacillus subtilis (Khan et al., Biosci.Biotechnol Biochem. 69:1861 1870 (2005)), Nicotiana tabacum (Purnell et al., Planta 222:167-180 (2005)), Oryza sativa 117 (Abiko et al., Plant Cell Physiol 46:1724-1734 (2005)), Haloferax mediterranei (Diaz et al., Extremophiles. 10:105-115 (2006)) and Halobactreium salinarum (Hayden et al., FEMSMicrobiol Lett. 211:37-41 (2002)). The Nicotiana tabacum enzyme is composed of alpha and beta subunits encoded by gdhl and gdh2 (Purnell et al., Planta 222:167-180 5 (2005)). Overexpression of the NADH-dependent glutamate dehydrogenase was found to improve ethanol production in engineered strains of S. cerevisiae (Roca et al., Appl Environ.Microbiol 69:4732-4736 (2003)). The ldh gene of Bacillus cereus encodes the LeuDH protein that accepts a wide of range of substrates including leucine, isoleucine, valine, and 2-aminobutanoate (Ansorge et al., Biotechnol Bioeng 68:557-562 (2000); 10 Stoyan et al., JBiotechnol 54:77-80 (1997)). The nadX gene from Thermotoga maritima encodes aspartate dehydrogenase, involved in the biosynthesis of NAD (Yang et al., J Biol.Chem. 278:8804-8808 (2003)). Protein GenBank ID GI Number Organism gdhA P00370 118547 Escherichia coli gdh P96110.4 6226595 Thermotoga maritima gdhAl NP 279651.1 15789827 Halobacterium salinarum rocG NP 391659.1 16080831 Bacillus subtilis gdh1 AAR11534.1 38146335 Nicotiana tabacum gdh2 AAR1535.1 38146337 Nicotiana tabacum GDH Q852M0 75243660 Oryza sativa GDH Q977U6 74499858 Haloferax mediterranei GDH P29051 118549 Halobactreium salinarum GDH2 NP 010066.1 6319986 Saccharomyces cerevisiae ldh P0A393 61222614 Bacillus cereus nadX NP 229443.1 15644391 Thennotoga maritima An exemplary enzyme for catalyzing the conversion of primary amines to their 15 corresponding aldehydes is lysine 6-dehydrogenase (EC 1.4.1.18), encoded by the lysDH genes. This enzyme catalyzes the oxidative deamination of the 6-amino group of L-lysine to form 2-aminoadipate-6-semialdehyde (Misono et al., JBacteriol. 150:398-401 (1982)). Exemplary lysine 6-dehydrogenase enzymes are found in Geobacillus stearothermophilus (Heydari et al., AEM 70:937-942 (2004)), Agrobacterium tumefaciens (Hashimoto et al., J 20 Biochem. 106:76-80 (1989); Misono and Nagasaki, JBacteriol. 150:398-401 (1982)), and Achromobacter denitrificans (Ruldeekulthamrong et al., BMB.Rep. 41:790-795 (2008)). Protein GenBank ID GI Number Organism lysDH BAB39707 13429872 Geobacillus stearothermophilus lysDH NP 353966 15888285 Agrobacterium tumefaciens lysDH AAZ94428 74026644 Achromobacter denitrificans 118 1.4.3.a Amine oxidase Amine.oxidase enzymes in EC class 1.4.3 catalyze the oxidative deamination of amino groups to their corresponding aldehydes or ketones. This class of enzymes utilizes oxygen 5 as the electron acceptor, converting an amine, 02 and water to an aldehyde or ketone, ammonia and hydrogen peroxide. L-Amino-acid oxidase catalyzes the oxidative deamination of a number of L-amino acids to their respective 2-oxoacids. The Streptococcus oligofermentans enzyme was overexpressed in E. coli (Tong et al, J Bacteriol 190:4716-21 (2008)). Other amine oxidase enzymes such as lysine-6-oxidase 10 (EC 1.4.3.20) and putrescine oxidase (EC 1.4.3.10), are specific to terminal amines. Lysine-6-oxidase enzymes are encoded by lodA of Marinomonas mediterranea (Lucas Elio et al, J Bacteriol 188:2493-501 (2006)) and alpP of Pseudoalteromonas tunicata (Mai-Prochnow et al, J Bacteriol 190:5493-501 (2008)). Putrescine oxidase enzymes are encoded by puo of Kocuria rosea (Ishizuka et al, J Gen Microbiol 139:425-32 (1993)) and 15 ATA01 of Arabidopsis thaliana (Moller and McPherson, PlantJ 13:781-91 (1998)). Protein GenBank ID GI Number Organism EU495328.1:1..1176 ACA52024.1 169260271 Streptococcus oligofermentans lodA AAY33849.1 83940756 Marinomonas mediterranea alpP AAP73876.1 32396307 Pseudoalteromonas tunicata puo BAA02074.1 303641 Kocuria rosea ATA01 AAB87690.1 2654118 Arabidopsisthaliana 2.3.1.a Acyltransferase (transferring phosphate group to CoA: phosphotransacylase) An enzyme with 2,4-pentadienoyl-CoA phosphotransferase activity is required to convert 20 2,4-pentadienoyl-CA to 2,4-pentadienoyl-phosphate (Figure 6, Step D). Exemplary phosphate-transferring acyltransferases include phosphotransacetylase (EC 2.3.1.8) and phosphotransbutyrylase (EC 2.3.1.19). The pta gene from E. coli encodes a phosphotransacetylase that reversibly converts acetyl-CoA into acetyl-phosphate (Suzuki, Biochim.Biophys.Acta 191:559-569 (1969)). This enzyme can also utilize propionyl-CoA 25 as a substrate, forming propionate in the process (Hesslinger et al., Mol.Microbiol 27:477 492 (1998)). Other phosphate acetyltransferases that exhibit activity on propionyl-CoA are found in Bacillus subtilis (Rado et al., Biochim.Biophys.Acta 321:114-125 (1973)), Clostridium kluyveri (Stadtman, Methods Enzymol 1:596-599 (1955)), and Thermotoga maritima (Bock et al., JBacteriol. 181:1861-1867 (1999)). Similarly, the ptb gene from C. 30 acetobutylicum encodes phosphotransbutyrylase, an enzyme that reversibly converts 119 butyryl-CoA into butyryl-phosphate (Wiesenborn et al., Appl Environ.Microbiol 55:317 322 (1989); Walter et al., Gene 134:107-111 (1993)). Additional ptb genes are found in butyrate-producing bacterium L2-50 (Louis et al., JBacteriol. 186:2099-2106 (2004)) and Bacillus megaterium (Vazquez et al., Curr.Microbiol 42:345-349 (2001)). Protein GenBankID GI Number Ormanism pta NP 416800,1 71152910 Escherichia coli pta P39646 730415 Bacillus subtilis pta A5N801 146346896 Clostridium kluyveri pta Q9XOL4 6685776 Thennotoga maritima ptb NP 349676 34540484 Clostridium acetobutylicum ptb AAR19757.1 38425288 butyrate-producing bacterium L2-50 ptb CAC07932.1 10046659 Bacillus megaterium 5 2.3.1.b Beta-ketothiolase Beta-ketothiolase enzymes in the EC class 2.3.1 catalyze the condensation of two acyl CoA substrates. Several transforms in Figures 2-4 require a beta-ketothiolase, including 10 step A of Figure 2, step A of Figure 3 and step A of Figure 4. Exemplary beta-ketothiolases with acetoacetyl-CoA thiolase activity include the gene products of atoB from E. coli (Martin et al., Nat.Biotechnol 21:796-802 (2003)), thiA and thIB from C. acetobutylicum (Hanai et al., Appl Environ Microbiol 73:7814-7818 (2007); 15 Winzer et al., J.Mol.Microbiol Biotechnol 2:531-541 (2000)), and ERG10 from S. cerevisiae (Hiser et al., J.Biol. Chem. 269:31383-31389 (1994)). Protein GenBank ID GI Number Organism atoB NP_416728 16130161 Escherichia coli thiA NP_349476.1 15896127 Clostridium acetobutylicum thIB NP_149242.1 15004782 Clostridium acetobutylicum ERG1O NP_015297 6325229 Saccharomyces cerevisiae Beta-ketothiolase enzymes catalyzing the formation of beta-ketovalerate from acetyl-CoA and propionyl-CoA are also suitable candidates. Zoogloea ramigera possesses two 20 ketothiolases that can form 3-ketovaleryl-CoA from propionyl-CoA and acetyl-CoA and R. eutropha has a beta-oxidation ketothiolase that is also capable of catalyzing this transformation (Gruys et al., US Patent 5,958,745 (1999)). The sequences of these genes 120 or their translated proteins have not been reported, but several candidates in R. eutropha, Z. ramigera, or other organisms can be identified based on sequence homology to bktB from R. eutropha. These include: Protein GenBank ID GI Number Organism phaA YP_725941.1 113867452 Ralstonia eutropha h16_AJ713 YP_726205.1 113867716 Ralstonia eutropha pcaF YP_728366.1 116694155 Ralstonia eutropha h16_B1369 YP_840888.1 116695312 Ralstonia eutropha h16_A0170 YP_724690.1 113866201 Ralstonia eutropha h16_A0462 YP_724980.1 113866491 Ralstonia eutropha h16_A1528 YP 726028.1 113867539 Ralstonia eutropha h16_B0381 YP_728545.1 116694334 Ralstonia eutropha h16_B0662 YP_728824.1 116694613 Ralstonia eutropha h16_B0759 YP_728921.1 116694710 Ralstonia eutropha h16_B0668 YP_728830.1 116694619 Ralstonia eutropha h6_A.1720 YP_726212.1 113867723 Ralstonia eutropha h16_A1887 YP_726356.1 113867867 Ralstonia eutropha phbA P07097.4 135759 Zoogloea ramigera bktB YP_002005382.1 194289475 Cupriavidus taiwanensis Rmet_1362 YP_583514.1 94310304 Ralstonia metallidurans Bphy_0975 YP_001857210.1 186475740 Burkholderia phymatum 5 Another suitable candidate is 3-oxoadipyl-CoA thiolase (EC 2.3.1.174), which converts beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA, and is a key enzyme of the beta ketoadipate pathway for aromatic compound degradation. The enzyme is widespread in soil bacteria and fungi including Pseudomonas putida (Harwood et al., JBacteriol. 176:6479-6488 (1994)) and Acinetobacter calcoaceticus (Doten et al., JBacteriol. 10 169:3168-3174 (1987)). The gene products encoded by pcaF in Pseudomonas strain B13 (Kaschabek et al., J Bacteriol. 184:207-215 (2002)), phaD in Pseudomonas putida U (Olivera et al., Proc.NatL.Acad.Sci U.S.A 95:6419-6424 (1998)), paaE in Pseudomonas fluorescens ST (Di et al., Arch.Microbiol 188:117-125 (2007)), and paaJ from E. coli (Nogales et al., Microbiology 153:357-365 (2007)) also catalyze this transformation. 15 Several beta-ketothiolases exhibit significant and selective activities in the oxoadipyl-CoA forming direction including bkt from Pseudomonas putida, pcaF and bkt from Pseudomonas aeruginosa PA01, bkt from Burkholderia aibifaria AMMD, paaJ from E. coli, and phaD from P. putida.

121 Gene name GI# GenBank Accession # Organism paaJ 16129358 NP 415915.1 Escherichia coli pcaF 17736947 AAL02407 Pseudomonas knackmussii (B13) phaD 3253200 AAC24332.1 Pseudomonasputida pcaF 506695 AAA85138.1 Pseudomonas putida pcaF 141777 AAC37148.1 Acinetobacter calcoaceticus paaE 106636097 ABF82237.1 Pseudomonas fluorescens bkt 115360515 YP 777652.1 Burkholderia ambifaria AMMD bkt 9949744 AAG06977.1 Pseudomonas aeruginosa PA01 pcaF 9946065 AAG3617.1 Pseudomonas aeruginosa PA01 2.3.1.d Formate C-acyltransferase Formate C-acyltransferase enzymes in the EC class 2.3.1 catalyze the acylation of 5 ketoacids and concurrent release of formate. Such an enzyme is suitable for the conversion of 2-oxoadipate to glutaryl-CoA in Figure 1 (step R) and the conversion of 4-hydroxy-2 oxovalerate to 3-hydroxybutyryl-CoA in step H of Figure 5. Enzymes in this class include pyruvate formate-lyase and ketoacid formate-lyase. Pyruvate formate-lyase (PFL, EC 2.3.1.54), encoded by pflB in E. coli, converts pyruvate into 10 acetyl-CoA and formate. The active site of PFL contains a catalytically essential glycyl radical that is posttranslationally activated under anaerobic conditions by PFL-activating enzyme (PFL-AE, EC 1.97.1.4) encoded by pflA (Knappe et al., Proc.Natl.Acad.Sci U.S.A 81:1332-1335 (1984); Wong et al., Biochemistry 32:14102-14110 (1993)). Keto-acid formate-lyase (EC 2.3.1.-), also known as 2-ketobutyrate formate-lyase (KFL) and 15 pyruvate formate-lyase 4, is the gene product of tdcE in E. coli. This enzyme catalyzes the conversion of 2-ketobutyrate to propionyl-CoA and formate during anaerobic threonine degradation, and can also substitute for pyruvate formate-lyase in anaerobic catabolism (Simanshu et al., JBiosci. 32:1195-1206 (2007)). The enzyme is oxygen-sensitive and, like PflB, requires post-translational modification by PFL-AE to activate a glycyl radical 20 in the active site (Hesslinger et al., Mol.Microbiol 27:477-492 (1998)). A pyruvate formate-lyase from Archaeglubusfulgidus encoded by pflD has been cloned, expressed in E. coli and characterized (Lehtio et al., Protein Eng Des Sel 17:545-552 (2004)). The crystal structures of the A.fulgidus and E. coli enzymes have been resolved (Lehtio et al., JMoL.Biol. 357:221-235 (2006); Leppanen et al., Structure. 7:733-744 (1999)). Additional 25 PFL and PFL-AE candidates are found in Lactococcus lactis (Melchiorsen et al., Appl Microbiol Biotechnol 58:338-344 (2002)), and Streptococcus mutans (Takahashi-Abbe et al., Oral.MicrobiollImmunol. 18:293-297 (2003)), Chlamydomonas reinhardii 122 (Hemschemeier et al., Eukaryot.Cell 7:518-526 (2008b); Atteia et al., J.Biol.Chem. 281:9909-9918 (2006)) and Clostridium pasteurianum (Weidner et al., JBacteriol. 178:2440-2444 (1996)). Protein GenBank ID GI Number Organism p*fB NP 415423 16128870 Escherichia coli Rfl4 NP 415422.1 16128869 Escherichia coli tdcE AAT48170.1 48994926 Escherichia coli pflD NP 070278.1 11499044 Archaeglubusfulgidus pfl CAA03993 2407931 Lactococcus lactis R9 BAA09085 1129082 Streptococcus muans PFL1 XP 001689719.1 159462978 Chlamydomonas reinhardtii pflA XP 001700657.1 159485246 Chlamydomonas reinhardtii flQ46266.1 2500058 Clostridium pasteurianum act CAA63749.1 1072362 Clostridium pasteurianum 5 2.3.1.h 3-Oxoacvl-CoA Synthase 3-Oxoacyl-CoA products such as acetoacetyl-CoA, 3-oxopentanoyl-CoA, 3-oxo-5 hydroxypentanoyl-CoA can be synthesized from acyl-CoA and malonyl-CoA substrates by 3-oxoacyl-CoA synthases (Steps 2A, 3A, 4A, 7AS). As enzymes in this class catalyze 10 an essentially irreversible reaction, they are particularly useful for metabolic engineering applications for overproducing metabolites, fuels or chemicals derived from 3-oxoacyl CoA intermediates such as acetoacetyl-CoA. Acetoacetyl-CoA synthase, for example, has been heterologously expressed in organisms that biosynthesize butanol (Lan et al, PNAS USA (2012)) and poly-(3-hydroxybutyrate) (Matsumoto et al, Biosci Biotech Biochem, 15 75:364-366 (2011). An acetoacetyl-CoA synthase (EC 2.3.1.194) enzyme (FhsA) has been characterized in the soil bacterium Streptomyces sp. CL 190 where it participates in mevalonate biosynthesis (Okamura et al, PNAS USA 107:11265-70 (2010)). Other acetoacetyl-CoA synthase genes can be identified by sequence homology toJhsA. Protein GenBank ID GI Number Organism fhsA BAJ83474.1 325302227 Streptomyces sp CL190 AB183750.1:1 BAD86806.1 57753876 Streptomyces sp. KO-3988 1991..12971 epzT ADQ43379.1 312190954 Streptomyces cinnamonensis ppzT CAX48662.1 238623523 Streptomyces anulatus 031 22085 ZP 09840373.1 378817444 Nocardia brasiliensis 20 123 2.6.1.a Aminotransferase Aminotransferases reversibly convert an amino group to an aldehyde or ketone. Exemplary enzymes for converting aldehydes to primary amines include lysine-6 aminotransferase (EC 2.6.1.36), 5-aminovalerate aminotransferase (EC 2.6.1.48), gamma 5 aminobutyrate aminotransferase and diamine aminotransferases such as putrescine aminotransferase (EC 2.6.1.82 and 2.6.1.29). These enzymes are suitable for catalyzing steps C and I of Figure 1. Aspartate aminotransferase and similar enzymes convert amino acids to their corresponding 2-ketoacids. Amino acid aminotransferases are suitable candidates for interconverting 2-oxoadipate and 2-aminoadipate (Step H of Figure 1). 10 Lysine-6-aminotransferase (EC 2.6.1.36) converts lysine to alpha-aminoadipate semialdehyde, and has been charactierized in yeast and bacteria. Candidates from Candida utilis (Hammer et al., J Basic Microbiol 32:21-27 (1992)), Flavobacterium lutescens (Fujii et al., JBiochem. 128:391-397 (2000)) and Streptomyces clavuligenus (Romero et al., J Ind.Microbiol Biotechnol 18:241-246 (1997)) have been characterized. A recombinant 15 lysine-6-aminotransferase from S. clavuligenus was functionally expressed in E. coli (Tobin et al., JBacteriol. 173:6223-6229 (1991)). The F. lutescens enzyme is specific to alpha-ketoglutarate as the amino acceptor (Soda et al., Biochemistry 7:4110-4119 (1968)). A related enzyme, diaminobutyrate aminotransferase (EC 2.6.1.46 and EC 2.6.1.76), is encoded by the dat gene products in Acinetobacter baumanii and Haemophilus influenza 20 (Ikai et al., JBacteriol. 179:5118-5125 (1997); Ikai et al., Biol Pharm.Bull. 21:170-173 (1998)). In addition to its natural substrate, 2,4-diaminobutyrate, the A. baumanii DAT transaminates the terminal amines of lysine, 4-aminobutyrate and ornithine. Additional diaminobutyrate aminotransferase gene candidates include the ectB gene products of Marinococcus halophilus and Halobacillus dabanensis (Zhao et al., Curr Microbiol 25 53:183-188 (2006); Louis et al., Microbiology 143 (Pt 4):1141-1149 (1997)) and the pvdH gene product of Pseudomonas aeruginosa (Vandenende et al., J Bacteriol. 186:5596-5602 (2004)). The beta-alanine aminotransferase of Pseudomonasfluorescens also accepts 2,4-diaminobutyrate as a substrate (Hayaishi et al., JBiol Chem 236:781-790 (1961)); however, this activity has not been associated with a gene to date. Gene GenBank ID GI Number Organism lat BAB13756.1 10336502 Flavobacterium lutescens lat AAA26777.1 153343 Streptomyces clavuligenus dat P56744.1 6685373 Acinetobacter baumanii dat P44951.1 1175339 Haemophilus influenzae ectB AAB57634.1 2098609 Marinococcus halophilus 124 ectB AAZ57191.1 171979940 Halobacillus dabanensis pvdH AAG05801.1 9948457 Pseudomonas aeruginosa The conversion of an aldehyde to a terminal amine can also be catalyzed by gamma aminobutyrate (GABA) transaminase (EC 2.6.1.19) or 5-aminovalerate transaminase. GABA aminotransferase interconverts succinic semialdehyde and glutamate to 4 5 aminobutyrate and alpha-ketoglutarate and is known to have a broad substrate range (Schulz et al., 56:1-6 (1990); Liu et al., 43:10896-10905 (2004)). The two GABA transaminases in E. coli are encoded by gabT (Bartsch et al., JBacteriol. 172:7035-7042 (1990)) and puuE (Kurihara et al., J.BioL.Chem. 280:4602-4608 (2005)). GABA transaminases in Mus musculus and Sus scrofa also catalyze the transamination of 10 alternate substrates including 6-aminocaproic acid (Cooper, Methods Enzymol. 113:80-82 (1985)). 5-Aminovalerate aminotransferase (EC 2.6.1.48) converts 5-aminovalerate to 5 oxovalerate during lysine degradation. The enzyme is encoded by davT of Pseudomonas putida (Espinosa-Urgel et al, Appl Env Microbiol, 67:5219-24 (2001)) and PA 0266 of Pseudomonas aeruginosa (Yamanishi et al, FEBSJ. 274:2262-73 (2007)). A 5 15 aminovalerate aminotransferase from Clostridium aminovalericum was purified and characterized but the sequence has not been published to date (Barker et al, J Biol Chem, 262:8994-9003 (1987)). Gene GenBank ID GI Number Organism gabT NP 417148.1 16130576 Escherichia coli puuE NP 415818.1 16129263 Escherichia coli Abat NP 766549.2 37202121 Mus musculus gabT YP 257332.1 70733692 Pseudomonasfluorescens Abat NP 999428.1 47523600 Sus scrofa davT AAK97868.1 15428718 Pseudomonasputida PA0266 NP 248957.1 15595463 Pseudomonas aeruginosa Putrescine aminotransferase (EC 2.6.1.82) and other diamine aminotransferases (EC 20 2.6.1.29) also catalyze the interconversion of aldehydes and primary amines. The E. coli putrescine aminotransferase is encoded by the ygjG gene and the purified enzyme transaminates the alternative substrates cadaverine, spermidine and 1,7-diaminoheptane (Samsonova et al., BMC.Microbiol 3:2 (2003)). Activity of this enzyme with amino acceptors other than 2-oxoglutarate (e.g., pyruvate, 2-oxobutanoate) has been reported 25 (Samsonova et al., BMC.Microbiol 3:2 (2003); KIM, JBiol.Chem. 239:783-786 (1964)). Another putrescine aminotransferase enzyme is encoded by spuC gene of Pseudomonas aeruginosa (Lu et al., JBacteriol. 184:3765-3773 (2002)).

125 Gene GenBank ID GI Number Organism ygjG NP 417544 145698310 Escherichiacoli spuC AAG03688 9946143 Pseudomonas aeruginosa Several aminotransferases transaminate the amino groups of amino acid groups to form 2 oxoacids. Aspartate aminotransferase is an enzyme that naturally transfers an oxo group 5 from oxaloacetate to glutamate, forming alpha-ketoglutarate and aspartate. Aspartate aminotransferase activity is catalyzed by, for example, the gene products of aspC from Escherichia coli (Yagi et al., 100:81-84 (1979); Yagi et al., 113:83-89 (1985)), AAT2 from Saccharomyces cerevisiae (Yagi et al., 92:35-43 (1982)) and ASP5 from Arabidopsis thaliana (Kwok et al., 55:595-604 (2004); de la et al., 46:414-425 (2006); 10 Wilkie et al., Protein Expr.Purif 12:381-389 (1998)). The enzyme from Rattus norvegicus has been shown to transaminate alternate substrates such as 2-aminohexanedioic acid and 2,4-diaminobutyric acid (Recasens et al., Biochemistry 19:4583-4589 (1980)). Aminotransferases that work on other amino-acid substrates may also be able to catalyze these transformations. Valine aminotransferase catalyzes the conversion of valine and 15 pyruvate to 2-ketoisovalerate and alanine. The E. coli gene, avtA, encodes one such enzyme (Whalen et al., J.Bacteriol. 150:739-746 (1982)). This gene product also catalyzes the transamination of a-ketobutyrate to generate a-aminobutyrate, although the amine donor in this reaction has not been identified (Whalen et al., J.Bacteriol. 158:571-574 (1984)). The gene product of the E. coli serC catalyzes two reactions, phosphoserine 20 aminotransferase and phosphohydroxythreonine aminotransferase (Lam et al., J.Bacteriol. 172:6518-6528 (1990)), and activity on non-phosphorylated substrates could not be detected (Drewke et al., FEBS.Lett. 390:179-182 (1996)). Another enzyme candidate is alpha-aminoadipate aminotransferase (EC 2.6.1.39), an enzyme that participates in lysine biosynthesis and degradation in some organisms. This enzyme interconverts 2 25 aminoadipate and 2-oxoadipate, using alpha-ketoglutarate as the amino acceptor. Gene candidates are found in Homo sapiens (Okuno et al., Enzyme Protein 47:136-148 (1993)) and Thermus thermophilus (Miyazaki et al., Microbiology 150:2327-2334 (2004)). The Thermus thermophilus enzyme, encoded by lysN, is active with several alternate substrates including oxaloacetate, 2-oxoisocaproate, 2-oxoisovalerate, and 2-oxo-3-methylvalerate. Protein GenBank ID GI Number Organism aspC NP_415448.1 16128895 Escherichia coli AA T2 P23542.3 1703040 Saccharomyces cerevisiae 126 ASP5 P46248.2 20532373 Arabidopsis thaliana got2 P00507 112987 Rattus norvegicus avtA YP_026231.1 49176374 Escherichia coli lysN BAC76939.1 31096548 Thermus thermophilus AadAT-II Q8N5Z0.2 46395904 Homo sapiens 2.7.2.a Phosphotransferase (kinase) Kinase or phosphotransferase enzymes in the EC class 2.7.2 transform carboxylic acids to 5 phosphonic acids with concurrent hydrolysis of one ATP. An enzyme with 2,4 pentadienoate kinase activity is required in step H of Figure 6. Exemplary enzyme candidates include butyrate kinase (EC 2.7.2.7), isobutyrate kinase (EC 2.7.2.14), aspartokinase (EC 2.7.2.4), acetate kinase (EC 2.7.2.1) and glycerate kinase. Butyrate kinase catalyzes the reversible conversion of butyryl-phosphate to butyrate during 10 acidogenesis in Clostridial species (Cary et al., Appl Environ Microbiol 56:1576-1583 (1990)). The Clostridium acetobutylicum enzyme is encoded by either of the two buk gene products (Huang et al., JMol.Microbiol Biotechnol 2:33-38 (2000)). Other butyrate kinase enzymes are found in C. butyricum and C. tetanomorphum (Twarog et al., JBacteriol. 86:112-117 (1963)). A related enzyme, isobutyrate kinase from Thermotoga maritima, 15 was expressed in E. coli and crystallized (Diao et al., JBacteriol. 191:2521-2529 (2009); Diao et al., Acta Crystallogr.D.Biol.Crystallogr. 59:1100-1102 (2003)). Aspartokinase catalyzes the ATP-dependent phosphorylation of aspartate and participates in the synthesis of several amino acids. The aspartokinase III enzyme in E. coli, encoded by lysC, has a broad substrate range and the catalytic residues involved in substrate specificity have been 20 elucidated (Keng et al., Arch Biochem Biophys 335:73-81 (1996)). Two additional kinases in E. coli are also acetate kinase and gamma-glutamyl kinase. The E. coli acetate kinase, encoded by ackA (Skarstedt et al., J.Biol. Chem. 251:6775-6783 (1976)), phosphorylates propionate in addition to acetate (Hesslinger et al., Mol.Microbiol 27:477-492 (1998)). The E. coli gamma-glutamyl kinase, encoded by proB (Smith et al., J.Bacteriol. 157:545 25 551 (1984)), phosphorylates the gamma carbonic acid group of glutamate. Protein GenBank ID GI Number Organism buki NP 349675 15896326 Clostridium acetobutylicum buk2 Q971I1 20137415 Clostridium acetobutylicum buk2 Q9X278.1 6685256 Thermotoga maritima 127 Protein GenBank ID GI Number Oranism lysC NP 418448.1 16131850 Escherichia coli ackA NP 416799.1 16130231 Escherichia coli proB NP 414777.1 16128228 Escherichia coli Glycerate kinase (EC 2.7.1.31) activates glycerate to glycerate-2-phosphate or glycerate-3 phosphate. Three classes of glycerate kinase have been identified. Enzymes in class I and II produce glycerate-2-phosphate, whereas the class III enzymes found in plants and yeast 5 produce glycerate-3-phosphate (Bartsch et al., FEBS Lett. 582:3025-3028 (2008)). In a recent study, class III glycerate kinase enzymes from Saccharomyces cerevisiae, Oryza sativa and Arabidopsis thaliana were heterologously expressed in E. coli and characterized (Bartsch et al., FEBSLett. 582:3025-3028 (2008)). This study also assayed the glxK gene product of E. coli for ability to form glycerate-3 -phosphate and found that 10 the enzyme can only catalyze the formation of glycerate-2-phosphate, in contrast to previous work (Doughty et al., JBiol.Chem. 241:568-572 (1966)). Protein GenBankID GI Number Ori!nism glxK AAC73616.1 1786724 Escherichia coli YGR205W AAS56599.1 45270436 Saccharomyces cerevisiae Os01g0682500 BAF05800.1 113533417 Oryza sativa Atig80380 BAH57057.1 227204411 Arabidopsisthaliana 2.8.3.a CoA transferase 15 Enzymes in the 2.8.3 family catalyze the reversible transfer of a CoA moiety from one molecule to another. Such a transformation is required by steps L, P and 0 of Figure 1, step F of Figure 3, step B of Figure 4, step J of Figure 5 and step F of Figure 6. Several CoA transferase enzymes have been described in the open literature and represent suitable 20 candidates for these steps. These are described below. Many transferases have broad specificity and thus can utilize CoA acceptors as diverse as acetate, succinate, propionate, butyrate, 2-methylacetoacetate, 3-ketohexanoate, 3 ketopentanoate, valerate, crotonate, 3-mercaptopropionate, propionate, vinylacetate, butyrate, among others. For example, an enzyme from Roseburia sp. A2-183 was shown 25 to have butyryl-CoA:acetate:CoA transferase and propionyl-CoA:acetate:CoA transferase activity (Charrier et al., Microbiology 152, 179-185 (2006)). Close homologs can be found in, for example, Roseburia intestinalis L1-82, Roseburia inulinivorans DSM 16841, 128 Eubacterium rectale ATCC 33656. Another enzyme with propionyl-CoA transferase activity can be found in Clostridium propionicum (Selmer et al., Eur JBiochem 269, 372 380 (2002)). This enzyme can use acetate, (R)-lactate, (S)-lactate, acrylate, and butyrate as the CoA acceptor (Selmer et al., Eur J Biochem 269, 372-380 (2002); Schweiger and 5 Buckel, FEBS Letters, 171(1) 79-84 (1984)). Close homologs can be found in, for example, Clostridium novyi NT, Clostridium beijerinckii NCIMB 8052, and Clostridium botulinum C str. Eklund. YgfH encodes a propionyl CoA:succinate CoA transferase in E. coli (Haller et al., Biochemistry, 39(16) 4622-4629). Close homologs can be found in, for example, Citrobacteryoungae ATCC 29220, Salmonella enterica subsp. arizonae 10 serovar, and Yersinia intermedia ATCC 29909. These proteins are identified below. Protein GenBankID GI Number Organism Achi AAX19660.1 60396828 Roseburia sp. A2-183 ROSINTL 182_07 ZP_04743841.2 257413684 Roseburia intestinalis 121 L1-82 ROSEINA2194_0 ZP_03755203.1 225377982 Roseburia inulinivorans 3642 EUBREC_3075 YP_002938937.1 238925420 Eubacterium rectale ATCC 33656 Pct CAB77207.1 7242549 Clostridium propionicum NT0ICX 2372 YP 878445.1 118444712 Clostridium novyi NT Cbei 4543 YP 001311608.1 150019354 Clostridium beijerinckii CBC_A0889 ZP_02621218.1 168186583 Clostridium botulinum C str. Eklund ygf NP 417395.1 16130821 Escherichia coli CIT292_04485 ZP_03838384.1 227334728 Citrobacter youngae A TCC 29220 SARI_04582 YP_001573497.1 161506385 Salmonella enterica subsp. arizonae serovar yinte0001_14430 ZP_04635364.1 238791727 Yersinia intermedia ATCC 29909 An additional candidate enzyme is the two-unit enzyme encoded by pcal and pcal in Pseudomonas, which has been shown to have 3-oxoadipyl-CoA/succinate transferase activity (Kaschabek et al., supra). Similar enzymes based on homology exist in 15 Acinetobacter sp. ADP1 (Kowalchuk et al., Gene 146:23-30 (1994)) and Streptomyces coelicolor. Additional exemplary succinyl-CoA:3:oxoacid-CoA transferases are present in Helicobacterpylori (Corthesy-Theulaz et al., J.Biol.Chem. 272:25659-25667 (1997)) and Bacillus subtilis (Stols et al., Protein.Expr.Purif 53:396-403 (2007)). These proteins are identified below.

129 Protein GenBank ID GI Number Organism pcal AAN69545.1 24985644 Pseudomonas putida pcaJ NP 746082.1 26990657 Pseudomonas putida pcaI YP 046368.1 50084858 Acinetobacter sp. ADPJ pcaJ AAC37147.1 141776 Acinetobacter sp. ADP1 pcal NP 630776.1 21224997 Streptomyces coelicolor pcaJ NP 630775.1 21224996 Streptomyces coelicolor HPAGJ 0676 YP 627417 108563101 Helicobacter pylori HPAGJ 0677 YP 627418 108563102 Helicobacter pylori ScoA NP 391778 16080950 Bacillus subtilis ScoB NP 391777 16080949 Bacillus subtilis A CoA transferase that can utilize acetate as the CoA acceptor is acetoacetyl-CoA transferase, encoded by the E. coli atoA (alpha subunit) and atoD (beta subunit) genes (Vanderwinkel et al., Biochem.Biophys.Res Commun. 33:902-908 (1968); Korolev et al., 5 Acta Crystallogr.D Biol Crystallogr. 58:2116-2121 (2002)). This enzyme has also been shown to transfer the CoA moiety to acetate from a variety of branched and linear acyl CoA substrates, including isobutyrate (Matthies et al., AppI Environ Microbiol 58:1435 1439 (1992)), valerate (Vanderwinkel et al., supra) and butanoate (Vanderwinkel et al., supra). Similar enzymes exist in Corynebacterium glutamicum ATCC 13032 (Duncan et 10 al., Appl Environ Microbiol 68:5186-5190 (2002)), Clostridium acetobutylicum (Cary et al., AppI Environ Microbiol 56:1576-1583 (1990)), and Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci.Biotechnol Biochem. 71:58-68 (2007)). These proteins are identified below. Protein GenBank ID GI Number Organism atoA P76459.1 2492994 Escherichia coli K12 atoD P76458.1 2492990 Escherichia coli K12 actA YP_226809.1 62391407 Corynebacterium glutamicum A TCC 13032 cg0592 YP_224801.1 62389399 Corynebacterium glutamicum ATCC 13032 ctA NP 149326.1 15004866 Clostridium acetobutylicum ct NP 149327.1 15004867 Clostridium acetobutylicum ctfA AAP42564.1 31075384 Clostridium saccharoperbutylacetonicum ctfB AAP42565.1 31075385 Clostridium saccharoperbutylacetonicum 15 Additional exemplary transferase candidates are catalyzed by the gene products of cat], cat2, and cat3 of Clostridium kluyveri which have been shown to exhibit succinyl-CoA, 4 hydroxybutyryl-CoA, and butyryl-CoA transferase activity, respectively (Seedorf et al., 130 supra; Sohling et al., Eur.JBiochem. 212:121-127 (1993); Sohling et al., JBacteriol. 178:871-880 (1996)). Similar CoA transferase activities are also present in Trichomonas vaginalis (van Grinsven et al., J.Biol.Chem. 283:1411-1418 (2008)) and Trypanosoma brucei (Riviere et al., J.Biol.Chem. 279:45337-45346 (2004)). These proteins are 5 identified below. Protein GenBank.ID GI Number Or-glanism catl P38946.1 729048 Clostridium kluyveri cat2 P38942.2 172046066 Clostridium kluyveri cat3 EDK35586.1 146349050 Clostridium luyveri TVAG 395550 XP 001330176 123975034 Trichomonas vaginalis G3 Tb]1.02.0290 XP 828352 71754875 Trypanosoma brucei The glutaconate-CoA-transferase (EC 2.8.3.12) enzyme from anaerobic bacterium Acidaminococcusfermentans reacts with diacid glutaconyl-CoA and 3-butenoyl-CoA (Mack et al., FEBS Lett. 405:209-212 (1997)). The genes encoding this enzyme are gctA 10 and gctB. This enzyme has reduced but detectable activity with other CoA derivatives including glutaryl-CoA, 2-hydroxyglutaryl-CoA, adipyl-CoA and acrylyl-CoA (Buckel et al., Eur.J.Biochem. 118:315-321 (1981)). The enzyme has been cloned and expressed in E. coli (Mack et al., Eur.J.Biochem. 226:41-51 (1994)). These proteins are identified below. Protein GenBankID GI Number Organisin getA CAA57199.1 559392 Acidaminococcus-fermentans gctB CAA57200.1 559393 Acidaminococcusfermentans 15 3.1.2.a CoA hydrolase Enzymes in the 3.1.2 family hydrolyze acyl-CoA molecules to their corresponding acids. Such a transformation is required by step 0 of Figure 1, step F of Figure 3, step B of Figure 4, step J of Figure 5 and step F of Figure 6. Several such enzymes have been 20 described in the literature and represent suitable candidates for these steps. For example, the enzyme encoded by acot12 from Rattus norvegicus brain (Robinson et al., Biochem.Biophys.Res.Commun. 71:959-965 (1976)) can react with butyryl-CoA, hexanoyl-CoA and malonyl-CoA. The human dicarboxylic acid thioesterase, encoded by acot8, exhibits activity on glutaryl-CoA, adipyl-CoA, suberyl-CoA, sebacyl-CoA, and 25 dodecanedioyl-CoA (Westin et al., J.BioL.Chem. 280:38125-38132 (2005)). The closest E. coli homolog to this enzyme, tesB, can also hydrolyze a range of CoA thiolesters (Naggert 131 et al., JBiol Chem 266:11044-11050 (1991)). A similar enzyme has also been characterized in the rat liver (Deana R., Biochem Int 26:767-773 (1992)). Additional enzymes with hydrolase activity in E. coli include ybgC, paaI, and ybdB (Kuznetsova, et al., FEMSMicrobiol Rev, 2005, 29(2):263-279; Song et al., JBiol Chem, 2006, 5 281(16):11028-38). Though its sequence has not been reported, the enzyme from the mitochondrion of the pea leaf has a broad substrate specificity, with demonstrated activity on acetyl-CoA, propionyl-CoA, butyryl-CoA, palmitoyl-CoA, oleoyl-CoA, succinyl-CoA, and crotonyl-CoA (Zeiher et at., Plant.Physiol. 94:20-27 (1990)) The acetyl-CoA hydrolase, ACH1, from S. cerevisiae represents another candidate hydrolase (Buu et al., 10 J.Biol.Chem. 278:17203-17209 (2003)). Protein GenBank Accession # GI# Organism acot12 NP 570103.1 18543355 Rattus norvegicus tesB NP 414986 16128437 Escherichia coli acot8 CAA15502 3191970 Homo sapiens acot8 NP 570112 51036669 Rattus norvegicus tesA NP 415027 16128478 Escherichia coli ybgC NP 415264 16128711 Escherichia coli paaI NP 415914 16129357 Escherichia coli ybdB NP 415129 16128580 Escherichia coli ACH I NP 009538 6319456 Saccharomyces cerevisiae Additional hydrolase enzymes include 3-hydroxyisobutyryl-CoA hydrolase which has been described to efficiently catalyze the conversion of 3-hydroxyisobutyryl-CoA to 3 hydroxyisobutyrate during valine degradation (Shimomura et al., JBiol Chem. 269:14248 15 14253 (1994)). Genes encoding this enzyme include hibch of Rattus norvegicus (Shimomura et al., Methods Enzymol 324:229-240 (2000)) and Homo sapiens (Shimomura et al., supra). Similar gene candidates can also be identified by sequence homology, including hibch of Saccharomyces cerevisiae and BC_2292 of Bacillus cereus. Protein GenBank Accession # GI# Organism hibch Q5XIE6.2 146324906 Rattus norvegicus hibch Q6NVYl.2 146324905 Homo sapiens hibch P28817.2 2506374 Saccharomyces cerevisiae BC 2292 AP09256 29895975 Bacillus cereus 20 Yet another candidate hydrolase is the glutaconate CoA-transferase from Acidaminococcusfermentans. This enzyme was transformed by site-directed mutagenesis into an acyl-CoA hydrolase with activity on glutaryl-CoA, acetyl-CoA and 3-butenoyl CoA (Mack et al., FEBS.Lett. 405:209-212 (1997)).This suggests that the enzymes 132 encoding succinyl-CoA:3-ketoacid-CoA transferases and acetoacetyl-CoA:acetyl-CoA transferases may also serve as candidates for this reaction step but would require certain mutations to change their function. GeneBank accession numbers for the gctA and gctB genes are listed above. 5 4.1.1.a Decarboxylase Decarboxylase enzymes in the EC class 4.1.1 are required to catalyze steps A, D, T and U of Figure 1, step D of Figure 2, step C of Figure 4, steps C and F of Figure 5 and step G of Figure 6. 10 The decarboxylation reactions of 2,4-pentadienoate to butadiene (step T of Figure 1 and step G of Figure 6) and 5-hydroxypent-2-enoate to 3-buten-1-ol (step U of Figure 1) are catalyzed by enoic acid decarboxylase enzymes. Exemplary enzymes are sorbic acid decarboxylase, aconitate decarboxylase, 4-oxalocrotonate decarboxylase and cinnamate decarboxylase. Sorbic acid decarboxylase converts sorbic acid to 1,3-pentadiene. Sorbic 15 acid decarboxylation by Aspergillus niger requires three genes: padA1, ohbA1, and sdrA (Plumridge et al. Fung. Genet. Bio, 47:683-692 (2010). PadA1 is annotated as a phenylacrylic acid decarboxylase, ohbA1 is a putative 4-hydroxybenzoic acid decarboxylase, and sdrA is a sorbic acid decarboxylase regulator. Additional species have also been shown to decarboxylate sorbic acid including several fungal and yeast species 20 (Kinderlerler and Hatton, Food Addit Contain., 7(5):657-69 (1990); Casas et al., Int J Food Micro., 94(l):93-96 (2004); Pinches and Apps, Int. J. Food Microbiol. 116: 182-185 (2007)). For example, Aspergillus oryzae and Neosartoryafischeri have been shown to decarboxylate sorbic acid and have close homologs to padA1, ohbA1, and sdrA. Gene name GenBankID GI Number Organism padAl XP 001390532.1 145235767 Aspergillus niger ohbA1 XP 001390534.1 145235771 Aspergillus niger sdrA XP 001390533.1 145235769 Aspergillus niger padAl XP 001818651.1 169768362 Aspergillus oryzae ohbA1 XP 001818650.1 169768360 Aspergillus oryzae sdrA XP 001818649.1 169768358 Aspergillus oryzae padAl XP 001261423.1 119482790 Neosartoryafischeri ohbAl XP 001261424.1 119482792 Neosartoryafischeri sdrA XP 001261422.1 119482788 Neosartoryafischeri 25 Aconitate decarboxylase (EC 4.1.1.6) catalyzes the final step in itaconate biosynthesis in a strain of Candida and also in the filamentous fungus Aspergillus terreus (Bonnarme et al.

133 JBacteriol. 177:3573-3578 (1995); Willke and Vorlop, Appl Microbiol. Biotechnol 56:289-295 (2001)). A cis-aconitate decarboxylase (CAD) (EC 4.1.16) has been purified and characterized from Aspergillus terreus (Dwiarti et al., J. Biosci. Bioeng. 94(1): 29-33 (2002)). Recently, the gene has been cloned and functionally characterized (Kanamasa et 5 al., Appl.Microbiol Biotechnol 80:223-229 (2008)) and (WO/2009/014437). Several close homologs of CAD are listed below (EP 2017344A1; WO 2009/014437 Al). The gene and protein sequence of CAD were reported previously (EP 2017344 Al; WO 2009/014437 Al), along with several close homologs listed in the table below. Gene name GenBanklD GI Number Or anism CAD XP 001209273 115385453 Aspergillus terreus XP 001217495 115402837 Aspergillus terreus XP 001209946 115386810 Aspergillus terreus BAE66063 83775944 Aspergillus oryzae XP 001393934 145242722 Aspergillus niger XP 391316 46139251 Gibberella zeae XP 001389415 145230213 Aspergillus niger XP 001383451 126133853 Pichia stipitis YP 891060 118473159 Mycobacterium smegmatis NP_961187 41408351 Mycobacterium avium subsp. pratuberculosis YP 880968 118466464 Mycobacterium avium I ZP 01648681 119882410 Salinispora arenicola 10 An additional class of decarboxylases has been characterized that catalyze the conversion of cinnamate (phenylacrylate) and substituted cinnamate derivatives to the corresponding styrene derivatives. These enzymes are common in a variety of organisms and specific genes encoding these enzymes that have been cloned and expressed in E. coli are: pad I from Saccharomyces cerevisae (Clausen et al., Gene 142:107-112 (1994)), pdc from 15 Lactobacillus plantarum (Barthelmebs et al., 67:1063-1069 (2001); Qi et al., Metab Eng 9:268-276 (2007); Rodriguez et al., J.Agric.Food Chem. 56:3068-3072 (2008)), pofK (pad) from Klebsiella oxytoca (Uchiyama et al., Biosci.Biotechnol.Biochem. 72:116-123 (2008); Hashidoko et al., Biosci.Biotech.Biochem. 58:217-218 (1994)), Pedicoccus pentosaceus (Barthelmebs et al., 67:1063-1069 (2001)), and padC from Bacillus subtilis 20 and Bacillus pumilus (Shingler et al., 174:711-724 (1992)). A ferulic acid decarboxylase from Pseudomonasfluorescens also has been purified and characterized (Huang et al., J.Bacteriol. 176:5912-5918 (1994)). Importantly, this class of enzymes have been shown to be stable and do not require either exogenous or internally bound co-factors, thus 134 making these enzymes ideally suitable for biotransformations (Sariaslani, Annu.Rev.Microbiol. 61:51-69 (2007)). Protein GenBank ID GI Number Organism pad! AAB64980.1 1165293 Saccharomyces cerevisae pdc AAC45282.1 1762616 Lactobacillus plantarum pad BAF65031.1 149941608 Klebsiella oxytoca padC NP 391320.1 16080493 Bacillus subtilis pad YP 804027.1 116492292 Pedicoccus pentosaceus pad CAC18719.1 11691810 Bacillus pumilus 4-Oxalocronate decarboxylase catalyzes the decarboxylation of 4-oxalocrotonate to 2 5 oxopentanoate. This enzyme has been isolated from numerous organisms and characterized. The decarboxylase typically functions in a complex with vinylpyruvate hydratase. Genes encoding this enzyme include dmpH and dmpE in Pseudomonas sp. (strain 600) (Shingler et al., 174:711-724 (1992)), xylII and xylIII from Pseudomonas putida (Kato et al., Arch.Microbiol 168:457-463 (1997); Stanley et al., Biochemistry 10 39:3514 (2000); Lian et al., J.Am.Chem.Soc. 116:10403-10411 (1994)) and ReutB5691 and Reut_B5692 from Ralstonia eutropha JMP134 (Hughes et al., J Bacteriol, 158:79-83 (1984)). The genes encoding the enzyme from Pseudomonas sp. (strain 600) have been cloned and expressed in E. coli (Shingler et al., J. Bacteriol. 174:711-724 (1992)). The 4 oxalocrotonate decarboxylase encoded by xylI in Pseudomonas putida functions in a 15 complex with vinylpyruvate hydratase. A recombinant form of this enzyme devoid of the hydratase activity and retaining wild type decarboxylase activity has been characterized (Stanley et al., Biochem. 39:718-26 (2000)). A similar enzyme is found in Ralstonia pickettii (formerly Pseudomonas pickettii) (Kukor et al., J Bacteriol. 173:4587-94 (1991)). Gene GenBank GI Number Organism dmpH CAA43228.1 45685 Pseudomonas sp. CF600 dmpE C A A43225.1 45682 Pseudomonas sp. CF600 xylII YP 709328.1 111116444 Pseudomonas putida xylIII YP 709353.1 111116469 Pseudomonas putida Reut B5691 Y P 299880.1 7 3539513 Ralstonia eutropha JMP134 Reut B5692 Y P 299881.1 735395 14 Ralstonia eutropha JMP134 xylI P49155.1 135 1446 Pseudomonas putida tbuI YP 002983475.1 1241665116 Ralstonia pickettii nbaG BAC65309.1 28971626 Pseudomonasfluorescens KU-7 20 The decarboxylation of 2-keto-acids such as 2-oxoadipate (step D of Figure 1) is catalyzed by a variety of enzymes with varied substrate specificities, including pyruvate 135 decarboxylase (EC 4.1.1.1), benzoylformate decarboxylase (EC 4.1.1.7), alpha ketoglutarate decarboxylase and branched-chain alpha-ketoacid decarboxylase. Pyruvate decarboxylase (PDC), also termed keto-acid decarboxylase, is a key enzyme in alcoholic fermentation, catalyzing the decarboxylation of pyruvate to acetaldehyde. The enzyme 5 from Saccharomyces cerevisiae has a broad substrate range for aliphatic 2-keto acids including 2-ketobutyrate, 2-ketovalerate, 3-hydroxypyruvate and 2-phenylpyruvate (22). This enzyme has been extensivelystudied, engineered for altered activity, and functionally expressed in E. coli (Killenberg-Jabs et al., EurJ.Biochem. 268:1698-1704 (2001); Li et al., Biochemistry. 38:10004-10012 (1999); ter Schure et al., Appl.Environ.Microbiol. 10 64:1303-1307 (1998)). The PDC from Zymomonas mobilus, encoded by pdc, also has a broad substrate range and has been a subject of directed engineering studies to alter the affinity for different substrates (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)). The crystal structure of this enzyme is available (Killenberg-Jabs et al., Eur.J.Biochem. 268:1698-1704 (2001)). Other well-characterized PDC candidates include the enzymes 15 from Acetobacterpasteurians (Chandra et al., 176:443-451 (2001)) and Kluyveromyces lactis (Krieger et al., 269:3256-3263 (2002)). Protein GenBank ID GI Number Organism pdc P06672.1 118391 Zymomonas mobilis pdcl P06169 30923172 Saccharomyces cerevisiae pdc Q8L388 20385191 Acetobacter pasteurians pdcl Q12629 52788279 Kluyveromyces lactis Like PDC, benzoylformate decarboxylase (EC 4.1.1.7) has a broad substrate range and has been the target of enzyme engineering studies. The enzyme from Pseudomonas putida has 20 been extensively studied and crystal structures of this enzyme are available (Polovnikova et al., 42:1820-1830 (2003); Hasson et al., 37:9918-9930 (1998)). Site-directed mutagenesis of two residues in the active site of the Pseudomonas putida enzyme altered the affinity (Kn) of naturally and non-naturally occurring substrates (Siegert et al., Protein Eng Des Sel 18:345-357 (2005)). The properties of this enzyme have been further 25 modified by directed engineering (Lingen et al., Chembiochem. 4:721-726 (2003); Lingen et al., Protein Eng 15:585-593 (2002)). The enzyme from Pseudomonas aeruginosa, encoded by mdiC, has also been characterized experimentally (Barrowman et al., 34:57 60 (1986)). Additional gene candidates from Pseudomonas stutzeri, Pseudomonas fluorescens and other organisms can be inferred by sequence homology or identified using 136 a growth selection system developed in Pseudomonas putida (Henning et al., Appl.Environ.Microbiol. 72:7510-7517 (2006)). Protein GenBank ID GI Number Organism mdlC P20906.2 3915757 Pseudomonas putida md/C Q9HLR2.1 81539678 Pseudomonas aeruginosa dpgB ABN80423.1 126202187 Pseudomonas stutzeri ilvB-1I YP 260581.1 70730840 Pseudomonasfiuorescens A third enzyme capable of decarboxylating 2-oxoacids is alpha-ketoglutarate 5 decarboxylase (KGD). The substrate range of this class of enzymes has not been studied to date. The KDC from Mycobacterium tuberculosis (Tian et al., 102:10670-10675 (2005)) has been cloned and functionally expressed. KDC enzyme activity has been detected in several species of rhizobia including Bradyrhizobiumjaponicum and Mesorhizobium loti (Green et al., 182:2838-2844 (2000)). Although the KDC-encoding gene(s) have not been 10 isolated in these organisms, the genome sequences are available and several genes in each genome are annotated as putative KDCs. A KDC from Euglena gracilis has also been characterized but the gene associated with this activity has not been identified to date (Shigeoka et al., Arch.Biochem.Biophys. 288:22-28 (1991)). The first twenty amino acids starting from the N-terminus were sequenced MTYKAPVKDVKFLLDKVFKV (SEQ ID 15 NO. ) (Shigeoka and Nakano, Arch.Biochem.Biophys 288:22-28 (1991)). The gene could be identified by testing candidate genes containing this N-terminal sequence for KDC activity. Protein GenBank ID GI Number Organism kgd 050463.4 160395583 Mycobacterium tuberculosis kgd NP 767092.1 27375563 Bradyrhizobiumjaponicum USDA11O kgd NP 105204.1 13473636 Mesorhizobium loti A fourth candidate enzyme for catalyzing this reaction is branched chain alpha-ketoacid 20 decarboxylase (BCKA). This class of enzyme has been shown to act on a variety of compounds varying in chain length from 3 to 6 carbons (Oku et al., JBiol Chem. 263:18386-18396 (1988); Smit et al., Appl Environ Microbiol 71:303-311 (2005)). The enzyme in Lactococcus lactis has been characterized on a variety of branched and linear substrates including 2-oxobutanoate, 2-oxohexanoate, 2-oxopentanoate, 3-methyl-2 25 oxobutanoate, 4-methyl-2-oxobutanoate and isocaproate (Smit et al., Appl Environ Microbiol 71:303-311 (2005)). The enzyme has been structurally characterized (Berg et al., Science. 318:1782-1786 (2007)). Sequence alignments between the Lactococcus lactis 137 enzyme and the pyruvate decarboxylase of Zymomonas mobilus indicate that the catalytic and substrate recognition residues are nearly identical (Siegert et at, Protein Eng Des Sel 18:345-357 (2005)), so this enzyme would be a promising candidate for directed engineering. Decarboxylation of alpha-ketoglutarate by a BCKA was detected in Bacillus 5 subtilis; however, this activity was low (5%) relative to activity on other branched-chain substrates (Oku and Kaneda, JBiol Chem. 263:18386-18396 (1988)) and the gene encoding this enzyme has not been identified to date. Additional BCKA gene candidates can be identified by homology to the Lactococcus lactis protein sequence. Many of the high-scoring BLASTp hits to this enzyme are annotated as indolepyruvate decarboxylases 10 (EC 4.1.1.74). Indolepyruvate decarboxylase (IPDA) is an enzyme that catalyzes the decarboxylation of indolepyruvate to indoleacetaldehyde in plants and plant bacteria. Recombinant branched chain alpha-keto acid decarboxylase enzymes derived from the El subunits of the mitochondrial branched-chain keto acid dehydrogenase complex from Homo sapiens and Bos taurus have been cloned and functionally expressed in E. coli 15 (Davie et al., J.Biol.Chem. 267:16601-16606 (1992); Wynn et al., J.Biol.Chem. 267:12400-12403 (1992); Wynn et al., J.Biol.Chem. 267:1881-1887 (1992)). In these studies, the authors found that co-expression of chaperonins GroEL and GroES enhanced the specific activity of the decarboxylase by 500-fold (Wynn et al., J.Biol.Chem. 267:12400-12403 (1992)). These enzymes are composed of two alpha and two beta 20 subunits. Protein GenBank ID GI Number Organism kdcA AAS49166.1 44921617 Lactococcus lactis BCKDHB NP 898871.1 34101272 Homo sapiens BCKDHA NP 000700.1 11386135 Homo sapiens BCKDHB P21839 115502434 Bos taurus BCKDHA P11178 129030 Bos taurus A decarboxylase enzyme suitable for decarboxylating 3-ketoacids is acetoacetate decarboxylase (EC 4.1.1.4). The enzyme from Clostridium acetobutylicum, encoded by adc, has a broad substrate specificity and has been shown to decarboxylate numerous 25 alternate substrates including 2-ketocyclohexane carboxylate, 3-oxopentanoate, 2-oxo-3 phenylpropionic acid, 2-methyl-3-oxobutyrate and benzoyl-acetate (Rozzel et al., J.Am.Chem.Soc. 106:4937-4941 (1984); Benner and Rozzell, J.Am.Chem.Soc. 103:993 994 (1981); Autor et al., JBiol.Chem. 245:5214-5222 (1970)). An acetoacetate decarboxylase has also been characterized in Clostridium beijerinckii (Ravagnani et al., 30 Mol.Microbiol 37:1172-1185 (2000)). The acetoacetate decarboxylase from Bacillus 138 polymyxa, characterized in cell-free extracts, also has a broad substrate specificity for 3 keto acids and can decarboxylate 3-oxopentanoate (Matiasek et al., Curr.Microbiol 42:276-281 (2001)). The gene encoding this enzyme has not been identified to date and the genome sequence of B. polymyxa is not yet available. Another adc is found in 5 Clostridium saccharoperbutylacetonicum (Kosaka, et al., Biosci.Biotechnol Biochem. 71:58-68 (2007)). Additional gene candidates in other organisms, including Clostridium botulinum and Bacillus amyloliquefaciens FZB42, can be identified by sequence homology. Protein GenBank ID GI Number Organism adc NP_149328.1 15004868 Clostridium acetobutylicum adc AAP42566.1 31075386 Clostridium saccharoperbutylacetonicum adc YP_001310906.1 150018652 Clostridium bejerinckii CLLA2135 YP001886324.1 187933144 Clostridium botulinum RBAM_030030 YP_001422565.1 154687404 Bacillus amyloliquefaciens 10 Numerous characterized enzymes decarboxylate amino acids and similar compounds, including aspartate decarboxylase, lysine decarboxylase and ornithine decarboxylase. Aspartate decarboxylase (EC 4.1.1.11) decarboxylates aspartate to form beta-alanine. This enzyme participates in pantothenate biosynthesis and is encoded by gene panD in Escherichia coli (Dusch et al., Appl.Environ.Microbiol 65:1530-1539 (1999); Ramjee et 15 al., Biochem.J 323 ( Pt 3):661-669 (1997); Merkel et al., FEMSMicrobiol Lett. 143:247 252 (1996); Schmitzberger et al., EMBO J22:6193-6204 (2003)). The enzymes from Mycobacterium tuberculosis (Chopra et al., Protein Expr.Purif 25:533-540 (2002)) and Corynebacterium glutanicum (Dusch et al., Appl.Environ.Microbiol 65:1530-1539 (1999)) have been expressed and characterized in E. coli. Protein GenBank ID GI Number Organism panD P0A790 67470411 Escherichia coli K12 panD Q9X4NO 18203593 Corynebacterium glutanicum panD P65660.1 54041701 Mycobacterium tuberculosis 20 Lysine decarboxylase (EC 4.1.1.18) catalyzes the decarboxylation of lysine to cadaverine. Two isozymes of this enzyme are encoded in the E. coli genome by genes cadA and ldcC.

139 CadA is involved in acid resistance and is subject to positive regulation by the cadC gene product (Lemonnier et al., Microbiology 144 ( Pt 3):751-760 (1998)). CadC accepts hydroxylysine and S-aminoethylcysteine as alternate substrates, and 2-aminopimelate and 6-aminocaproate act as competitive inhibitors to this enzyme (Sabo et al., Biochemistry 5 13:662-670 (1974)). The constitutively expressed Idc gene product is less active than CadA (Lemonnier and Lane, Microbiology 144 (Pt 3):751-760 (1998)). A lysine decarboxylase analogous to CadA was recently identified in Vibrio parahaemolyticus (Tanaka et al., JAppl Microbiol 104:1283-1293 (2008)). The lysine decarboxylase from Selenomonas ruminantium, encoded by ldc, bears sequence similarity to eukaryotic 10 ornithine decarboxylases, and accepts both L-lysine and L-ornithine as substrates (Takatsuka et al., Biosci.Biotechnol Biochem. 63:1843-1846 (1999)). Active site residues were identified and engineered to alter the substrate specificity of the enzyme (Takatsuka et al., JBacteriol. 182:6732-6741 (2000)). Several ornithine decarboxylase enzymes (EC 4.1.1.17) also exhibit activity on lysine and other similar compounds. Such enzymes are 15 found in Nicotiana glutinosa (Lee et al., Biochem.J 360:657-665 (2001)), Lactobacillus sp. 30a (Guirard et al., JBiol.Chem. 255:5960-5964 (1980)) and Vibrio vulnficus (Lee et al., JBiol Chem. 282:27115-27125 (2007)). The enzymes from Lactobacillus sp. 30a (Momany et al., JMol.Biol. 252:643-655 (1995)) and V. vulnficus have been crystallized. The V. vulnficus enzyme efficiently catalyzes lysine decarboxylation and the residues 20 involved in substrate specificity have been elucidated (Lee et al., JBiol.Chem. 282:27115 27125 (2007)). A similar enzyme has been characterized in Trichomonas vaginalis but the gene encoding this enzyme is not known (Yarlett et al., Biochem.J 293 ( Pt 2):487-493 (1993)). Protein GenBank ID GI Number Organism cadA AAA23536.1 145458 Escherichia coli ldcC AAC73297.1 1786384 Escherichia coli ldc 050657.1 13124043 Selenomonas ruminantium cadA AB124819.1 44886078 Vibrio parahaemolyticus AF323910.1:1..1299 AAG45222.1 12007488 Nicotiana glutinosa odcl P43099.2 1169251 Lactobacillus sp. 30a VV2 1235 NP 763142.1 27367615 Vibrio vulnificus 25 Glutaryl-CoA dehydrogenase (GCD, EC 1.3.99.7 and EC 4.1.1.70) is a bifunctional enzyme that catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA (Figure 3, step 3). Bifunctional GCD enzymes are homotetramers that utilize electron transfer flavoprotein as an electron acceptor (Hartel et al., Arch.Microbiol 159:174-181 140 (1993)). Such enzymes were first characterized in cell extracts of Pseudomonas strains KB740 and K172 during growth on aromatic compounds (Hartel et al., Arch.Microbiol 159:174-181 (1993)), but the associated genes in these organisms is unknown. Genes encoding glutaryl-CoA dehydrogenase (gcdH) and its cognate transcriptional regulator 5 (gcdR) were identified in Azoarcus sp. CIB (Blazquez et al., Environ.Microbiol 10:474 482 (2008)). An Azoarcus strain deficient in gcdH activity was used to identify a heterologous gcdH gene from Pseudomonas putida (Blazquez et al., Environ.Microbiol 10:474-482 (2008)). The cognate transcriptional regulator in Pseudomonas putida has not been identified but the locus PP_0157 has a high sequence homology (> 69% identity) to 10 the Azoarcus enzyme. Additional GCD enzymes are found in Pseudomonasfuorescens and Paracoccus denitrificans (Husain et al., JBacteriol. 163:709-715 (1985)). The human GCD has been extensively studied, overexpressed in E. coli (Dwyer et al., Biochemistry 39:11488-11499 (2000)), crystallized, and the catalytic mechanism involving a conserved glutamate residue in the active site has been described (Fu et al., Biochemistry 43:9674 15 9684 (2004)). A GCD in Syntrophus aciditrophicus operates in the C0 2 -assimilating direction during growth on crotonate (Mouttaki et al., 73:930-938 (2007))). Two GCD genes in S. aciditrophicus were identified by protein sequence homology to the Azoarcus GcdH: syn_00480 (31%) and syn_01146 (31%). No significant homology was found to the Azoarcus GcdR regulatory protein. Protein GenBank ID GI Number Organism gcdH ABM69268.1 123187384 Azoarcus sp. CIB gcdR ABM69269.1 123187385 Azoarcus sp. CIB gcdH AAN65791.1 24981507 Pseudomonas putida KT2440 PP 0157 (gcdR) AAN65790.1 24981506 Pseudomonas putida KT2440 gcdH YP 257269.1 70733629 Pseudomonas fluorescens Pf-5 gcvA (gcdR) YP 257268.1 70733628 Pseudomonas fluorescens Pf-5 gcd YP 918172.1 119387117 Paracoccus denitrificans gcdR YP 918173.1 119387118 Paracoccus denitrificans gcd AAH02579.1 12803505 Homo sapiens syn 00480 ABC77899 85722956 Syntrophus aciditrophicus syn 01146 ABC76260 85721317 Syntrophus aciditrophicus 20 Alternatively, the carboxylation of crotonyl-CoA to glutaconyl-CoA and subsequent reduction to glutaryl-CoA can be catalyzed by separate enzymes: glutaconyl-CoA decarboxylase and glutaconyl-CoA reductase. Glutaconyl-CoA decarboxylase enzymes, characterized in glutamate-fermenting anaerobic bacteria, are sodium-ion translocating 25 decarboxylases that utilize biotin as a cofactor and are composed of four subunits (alpha, beta, gamma, and delta) (Boiangiu et al., JMoL.Microbiol Biotechnol 10:105-119 (2005); 141 Buckel, Biochim.Biophys.Acta 1505:15-27 (2001)). Such enzymes have been characterized in Fusobacterium nucleatum (Beatrix et al., Arch.Microbiol 154:362-369 (1990)) and Acidaminococcusfermentans (Braune et al., Mol.Microbiol 31:473-487 (1999)). Analogs to the F. nucleatum glutaconyl-CoA decarboxylase alpha, beta and delta 5 subunits are found in S. aciditrophicus. A gene annotated as an enoyl-CoA dehydrogenase, syn_00480, another GCD, is located in a predicted operon between a biotin-carboxyl carrier (syn_00479) and a glutaconyl-CoA decarboxylase alpha subunit (syn_00481). The protein sequences for exemplary gene products can be found using the following GenBank accession numbers shown below. Enoyl-CoA reductase enzymes are described above (see 10 EC 1.3.1). Protein GenBank ID GI Number Organism gcdA CAA49210 49182 Acidaminococcusfermentans gcdC AAC69172 3777506 Acidaminococcusfennentans gcdD AAC69171 3777505 Acidaminococcusfermentans gcdB AAC69173 3777507 Acidaminococcus fermentans FNO200 AAL94406 19713641 Fusobacterium nucleatum FNO201 AAL94407 19713642 Fusobacterium nucleatum FN0204 AAL94410 19713645 Fusobacterium nucleatum syn 00479 YP 462066 85859864 Syntrophus aciditrophicus syn 00481 YP 462068 85859866 Syntrophus aciditrophicus syn 01431 YP 460282 85858080 Syntrophus aciditrophicus syn 00480 ABC77899 85722956 Syntrophus aciditrophicus 4.1.1.b Decarboxylase, alkene forming An olef'm-forming decarboxylase enzyme catalyzes the conversion of 5-hydroxyvalerate to 15 3-buten- 1 -ol (step W of Figure 1). A terminal olefin-forming fatty acid decarboxylase is encoded by the oleT gene product of Jeotgalicoccus sp. ATCC8456 (Rude et al, AEM 77(5):1718-27 (2011)). This recently discovered enzyme is a member of the cytochrome P450 family of enzymes and is similar to P450s that catalyze fatty acid hydroxylation. OleT and homologs are listed in the table below. Additional fatty acid decarboxylase 20 enzymes are found in US 2011/0196180. Protein GenBank ID GI Number Organism oleT ADW41779.1 320526718 Jeotgalicoccus sp, ATCC8456 MCCL 0804 BAH 17511.1 222120176 Macrococcus caseolyticus SPSE_1582 ADX76840.1 323464687 Staphylococcus pseudintermedius faaH ADC49546.1 288545663 Bacillus pseudofirmus cypC2 EGQ19322.1 339614630 Sporosarcina newyorkensis 142 cypC BAK15372.1 32743900 Solibacillus silvestris Bcoam 010100017440 ZP 03227611.1 205374818 Bacillus coahuilensis 4.1.99.a Decarbonylase The conversion of penta-2,4-dienal to butadiene is catalyzed by a decarbonylase (Step B 5 of Figure 6). Decarbonylase enzymes catalyze the final step of alkane biosynthesis in plants, mammals, and bacteria (Dennis et al., Arch.Biochem.Biophys. 287:268-275 (1991)). Non-oxidative decarbonylases transfom aldehydes into alkanes with the concurrent release of CO. Exemplary decarbonylase enzymes include octadecanal decarbonylase (EC 4.1.99.5), sterol desaturase and fatty aldehyde decarbonylase. A cobalt 10 porphyrin containing decarbonylase was purified and characterized in the algae Botryococcus braunii; however, no gene is associated with this activity to date (Dennis et al., Proc.Natl.Acad.Sci.US.A 89:5306-5310 (1992)). A copper-containing decarbonylase from Pisum sativum was also purified and characterized (Schneider-Belhaddad et al., A rch.Biochem.Biophys. 377:341-349 (2000)). The CER1 gene of Arabidopsis thaliana 15 encodes a fatty acid decarbonylase involved in epicuticular wax formation (US 6,437,218). Additional fatty acid decarbonylases are found in Medicago truncatula, Vitis vinifera and Oryza sativa (US Patent Application 2009/0061493). Protein GenBank ID GI Number Oranism CERI NP 850932 145361948 Arabidopsis thaliana MtrDRAFT AC153128g2v2 ABN07985 124359969 Medicago truncatula VITISV 029045 CAN60676 147781102 Vitis vinifera OSJNBa0004N05.14 CAE03390.2 38345317 Oryza sativa Alternately, an oxidative decarbonylase can convert an aldehyde into an alkane. Oxidative 20 decarbonylases are cytochrome P450 enzymes that utilize NADPH and 02 as cofactbrs and release CO 2 , water and NADP*. This activity was demonstrated in the CYP4G2v1 and CYP4G~gene products of Musca domestica and Drosophila melanogaster (US Patent Application 2010/0136595). Additional enzyme candidates with oxidative decarbonylase activity can be identified in other organisms, for example Mamestra brassicae, 25 Helicoverpa zea and Acyrthosiphon pisum, by sequence homology. Protein GenBank ID GI Number Organism CYP4G2vl ABV48808.1 157382740 Musca domestica CYP4G1 NP 525031.1 17933498 Drosophila melanogaster CYP4G25 BAD81026.1 56710314 Antheraea yamamai CYP4M6 AAM54722.1 21552585 Helicoverpa zea 143 Protein GenBank ID GI Number Organism LOC100164072 XP 001944205.1 193650239 Acyrthosiphon pisum 4.1.3.a Lyase The condensation of pyruvate and acetaldehyde to 4-hydroxy-2-oxovalerate (Step A of 5 Figure 5) is catalyzed by 4-hydroxy-2-oxovalerate aldolase (EC 4.1.3.39). This enzyme participates in pathways for the degradation of phenols, cresols and catechols. The E. coli enzyme, encoded by mhpE, is highly specific for acetaldehyde as an acceptor but accepts the alternate substrates 2-ketobutyrate or phenylpyruvate as donors (Pollard et al., Appl Environ Microbiol 64:4093-4094 (1998)). Similar enzymes are encoded by the cmtG and 10 todH genes of Pseudomonas putida (Lau et al., Gene 146:7-13 (1994); Eaton, JBacteriol. 178:1351-1362 (1996)). In Pseudomonas CF600, this enzyme is part of a bifunctional aldolase-dehydrogenase heterodimer encoded by dmpFG (Manjasetty et al., Acta Crystallogr.D.Biol Crystallogr. 57:582-585 (2001)). The dehydrogenase functionality interconverts acetaldehyde and acetyl-CoA, providing the advantage of reduced cellular 15 concentrations of acetaldehyde, toxic to some cells. A similar aldolase-dehydrogenase complex is encoded by BphIJ of Burkholderia xenovorans (Baker et al, Biochem 48:655 1 8 (2009)). Gene GenBank ID GI Number Organism mhpE AAC73455.1 1786548 Escherichia coli cmtG AAB62295.1 1263190 Pseudomonas putida todH AAA61944.1 485740 Pseudomonas putida dmpG CAA43227.1 45684 Pseudomonas sp. CF600 dmpF CAA43226.1 45683 Pseudomonas sp. CF600 bphl ABE37049.1 91693852 Burkholderia xenovorans bphJ ABE37050.1 91693853 Burkholderia xenovorans 4.2.1.a Hydro-Ivase 20 The removal of water to form a double bond is catalyzed by dehydratase enzymes in the 4.2.1 family of enzymes. Hydratase enzymes are sometimes reversible and also catalyze dehydration. Dehydratase enzymes are sometimes reversible and also catalyze hydration. The removal of water from a given substrate is required by steps G, N and V of Figure 1, step C of Figure 2, step C of Figure 3 and steps B and E of Figure 5. Several hydratase and 25 dehydratase enzymes have been described in the literature and represent suitable candidates for these steps.

144 For example, many dehydratase enzymes catalyze the alpha, beta-elimination of water which involves activation of the alpha-hydrogen by an electron-withdrawing carbonyl, carboxylate, or CoA-thiol ester group and removal of the hydroxyl group from the beta position (Buckel et al, J Bacteriol, 117:1248-60 (1974); Martins et al, PNAS 101:15645-9 5 (2004)). Exemplary enzymes include 2-(hydroxymethyl)glutarate dehydratase (EC 4.2.1.

), fumarase (EC 4.2.1.2), 3-dehydroquinate dehydratase (EC 4.2.1.10), cyclohexanone hydratase (EC 4.2.1.-) and 2-keto-4-pentenoate dehydratase (EC 4.2.1.80), citramalate hydrolyase and dimethylmaleate hydratase. 2-(Hydroxymethyl)glutarate dehydratase is a [4Fe-4S]-containing enzyme that dehydrates 10 2-(hydroxymethyl)glutarate to 2-methylene-glutarate, studied for its role in nicontinate catabolism in Eubacterium barkeri (formerly Clostridium barkeri) (Alhapel et al., Proc Natl Acad Sci 103:12341-6 (2006)). Similar enzymes with high sequence homology are found in Bacteroides capillosus, Anaerotruncus colihominis, and Natranaerobius thermophilius. These enzymes are homologous to the alpha and beta subunits of [4Fe-4S] 15 containing bacterial serine dehydratases (e.g., E. coli enzymes encoded by tdcG, sdhB, and sdaA). An enzyme with similar functionality in E. barker is dimethylmaleate hydratase, a reversible Fe 2 '-dependent and oxygen-sensitive enzyme in the aconitase family that hydrates dimethylmaeate to form (2R,3S)-2,3-dimcthylmalate. This enzyme is encoded by dmdAB (Alhapel et al., Proc Nati Acad Sci U SA 103:12341-6 (2006); Kollmann-Koch et 20 al., Hoppe Seylers.Z.Physiol Chem. 365:847-857 (1984)). Protein GenBank ID GI Number Organism hmd ABC88407.1 86278275 Eubacterium barkeri BACCAP 02294 ZP 02036683.1 154498305 Bacteroides capillosus ANACOL_02527 ZP_02443222.1 167771169 Anaerotruncus colihominis NtherDRAFT_2368 ZP_02852366.1 169192667 Natranaerobius thermophilus dmdA ABC88408 86278276 Eubacterium barkeri dmdB ABC88409 86278277 Eubacterium barker Fumarate hydratase (EC 4.2.1.2) enzymes naturally catalyze the reversible hydration of fumarate to malate. Although the ability of fumarate hydratase to react with 3-oxobutanol as a substrate has not been described in the literature, a wealth of structural information is 25 available for this enzyme and other researchers have successfully engineered the enzyme to alter activity, inhibition and localization (Weaver, 61:1395-1401 (2005)). E. coli has three fumarases: FumA, FumB, and FumC that are regulated by growth conditions. FumB 145 is oxygen sensitive and only active under anaerobic conditions. FumA is active under microanaerobic conditions, and FumC is the only active enzyme in aerobic growth (Tseng et al., JBacteriol, 183:461-467 (200 1); Woods et al., 954:14-26 (1988); Guest et al., J Gen Microbiol 131:2971-2984 (1985)). Additional enzyme candidates are found in 5 Campylobacterjejuni (Smith et al., Int.JBiochem.Cell Biol 31:961-975 (1999)), Thernus thermophilus (Mizobata et al., Arch.Biochem.Biophys. 355:49-55 (1998)) and Rattus norvegicus (Kobayashi et al., J. Biochem, 89:1923-1931 (1981)). Similar enzymes with high sequence homology includefuml from Arabidopsis thaliana andfumC from Corynebacterium glutamicum. The MmcBC fumarase from Pelotomaculum 10 thermopropionicum is another class of fumarase with two subunits (Shimoyama et al., FEMSMicrobiol Lett, 270:207-213 (2007)). Protein GenBank ID GI Number Ornanism fumA NP 416129.1 16129570 Escherichia coli fiumB NP 418546.1 16131948 Escherichia coli fumC NP 416128.1 16129569 Escherichia coli fMC 069294 9789756 Campylobacterjejuni fumC P84127 75427690 Thermus thennophilus fumH P14408 120605 Rattus norvegicus fumi P93033 39931311 Arabidopsis thaliana fumC Q8NRN8 39931596 Corynebacterium glutamicum MmcB YP_001211906 147677691 Pelotomaculum thermopropionicum MmcC YP_001211907 147677692 Pelotomaculum 1_ 1 thermopropionicum Dehydration of 4-hydroxy-2-oxovalerate to 2-oxopentenoate is catalyzed by 4-hydroxy-2 oxovalerate hydratase (EC 4.2.1.80). This enzyme participates in aromatic degradation 15 pathways and is typically co-transcribed with a gene encoding an enzyme with 4-hydroxy 2-oxovalerate aldolase activity. Exemplary gene products are encoded by mhpD of E. coli (Ferrandez et al., JBacteriol. 179:2573-2581 (1997); Pollard et al., EurJBiochem. 251:98-106 (1998)), todG and cmtF of Pseudomonasputida (Lau et al., Gene 146:7-13 (1994); Eaton, JBacteriol. 178:1351-1362 (1996)), cnbE of Comamonas sp. CNB-1 (Ma 20 et al., Appi Environ Microbiol 73:4477-4483 (2007)) and mhpD of Burkholderia xenovorans (Wang et al., FEBSJ272:966-974 (2005)). A closely related enzyme, 2 oxohepta-4-ene- 1,7-dioate hydratase, participates in 4-hydroxyphenyl acetic acid degradation, where it converts 2-oxo-hept-4-ene-1,7-dioate (OHED) to 2-oxo-4-hydroxy hepta-1,7-dioate using magnesium as a cofactor (Burks et al., J.Am.Chem.Soc. 120: 146 (1998)). OHED hydratase enzyme candidates have been identified and characterized in E. coli C (Roper et al., Gene 156:47-51 (1995); Izumi et al., JMoL.Biol. 370:899-911 (2007)) and E. coli W (Prieto et al., JBacteriol. 178:111-120 (1996)). Sequence comparison reveals homologs in a wide range of bacteria, plants and animals. Enzymes with highly 5 similar sequences are contained in Klebsiella pneumonia (91% identity, eval = 2e-138) and Salmonella enterica (91% identity, eval = 4e- 138), among others. Protein GenBank Accession No. GI No. Organism mhpD AAC73453.2 87081722 Escherichia coli cmtF AAB62293.1 1263188 Pseudomonas putida todG AAA61942.1 485738 Pseudomonas putida cnbE YP 001967714.1 190572008 Comamonas sp. CNB-1 mhpD Q13VUO 123358582 Burkholderia xenovorans hpcG CAA57202.1 556840 Escherichia coli C hpaH CAA86044.1 757830 Escherichia coli W hpaH ABR80130.1 150958100 Klebsiella pneumoniae Sari 01896 ABX21779.1 160865156 Salmonella enterica Another enzyme candidate is citramalate hydrolyase (EC 4.2.1.34), an enzyme that naturally dehydrates 2-methylmalate to mesaconate. This enzyme has been studied in 10 Methanocaldococcusjannaschii in the context of the pyruvate pathway to 2-oxobutanoate, where it has been shown to have a broad substrate range (Drevland et al., JBacteriol. 189:4391-4400 (2007)). This enzyme activity was also detected in Clostridium tetanomorphum, Morganella morganii, Citrobacter amalonaticus where it is thought to participate in glutamate degradation (Kato et al., Arch.Microbiol 168:457-463 (1997)). 15 The M. jannaschii protein sequence does not bear significant homology to genes in these organisms. Protein GenBank ID GI Number Organism leuD | Q58673.1 3122345 Methanocaldococcusjannaschii Dimethylmaleate hydratase (EC 4.2.1.85) is a reversible Fe 2 -dependent and oxygen sensitive enzyme in the aconitase family that hydrates dimethylmaeate to form (2R,3S) 20 2,3-dimethylmalate. This enzyme is encoded by dmrdAB in Eubacterium barkeri (Alhapel et al., supra; Kollmann-Koch et al., Hoppe Seylers.Z.Physiol Chem. 365:847-857 (1984)). Protein GenBank ID GI Number Organism dmdA ABC88408 86278276 Eubacterium barkeri dmdB ABC88409.1 86278277 Eubacterium barkeri 147 Oleate hydratases represent additional suitable candidates as suggested in W02011076691. Examples include the following proteins. Protein GenBank ID GI Number Organism OhyA ACT54545.1 254031735 Elizabethkingia meningoseptica HMPREF0841_1446 ZP_07461147.1 306827879 Streptococcus pyogenes A TCC 10782 P700755_13397 ZP_01252267.1 91215295 Psychroflexus torquis ATCC 700755 RPB_2430 YP_486046.1 86749550 Rhodopseudomonas I I I palustris Enoyl-CoA hydratases (EC 4.2.1.17) catalyze the dehydration of a range of 3 5 hydroxyacyl-CoA substrates (Roberts et al., Arch.Microbiol 117:99-108 (1978); Agnihotri et al., Bioorg.Med.Chem. 11:9-20 (2003); Conrad et al., JBacteriol. 118:103-111 (1974)). The enoyl-CoA hydratase of Pseudomonas putida, encoded by ech, catalyzes the conversion of 3-hydroxybutyryl-CoA to crotonyl-CoA (Roberts et al., Arch.Microbiol 117:99-108 (1978)). This transformation is also catalyzed by the crt gene product of 10 Clostridium acetobutylicum, the crtl gene product of C. kluyveri, and other clostridial organisms Atsumi et al., Metab Eng 10:305-311 (2008); Boynton et al., JBacteriol. 178:3015-3024 (1996); Hillmer et al., FEBS Lett. 21:351-354 (1972)). Additional enoy[ CoA hydratase candidates are phaA and phaB, of P. putida, and paaA and paaB from P. fluorescens (Olivera et al., Proc.Natl.Acad.Sci U.S.A 95:6419-6424 (1998)). The gene 15 product ofpimF in Rhodopseudomonas palustris is predicted to encode an enoyl-CoA hydratase that participates in pimeloyl-CoA degradation (Harrison et al., Microbiology 151:727-736 (2005)). Lastly, a number of Escherichia coli genes have been shown to demonstrate enoyl-CoA hydratase functionality including maoC (Park et al., JBacteriol. 185:5391-5397 (2003)), paaF (Ismail et al., Eur.JBiochem. 270:3047-3054 (2003); Park 20 et al., Appl.Biochem.Biotechnol 113-116:335-346 (2004); Park et al., Biotechnol Bioeng 86:681-686 (2004)) and paaG (Ismail et al., Eur.JBiochem. 270:3047-3054 (2003); Park and Lee, Appl.Biochem.Biotechnol 113-116:335-346 (2004); Park and Yup, Biotechnol Bioeng 86:681-686 (2004)). Protein GenBank Accession No. GI No. Organism ech NP 745498.1 26990073 Pseudomonas putida crt NP 349318.1 15895969 Clostridium acetobutylicum crtl YP 001393856 153953091 Clostridium kluyveri phaA ABF82233.1 26990002 Pseudomonas putida phaB ABF82234.1 26990001 Pseudomonas putida 148 paaA NP 745427.1 106636093 Pseudomonasfluorescens paaB NP 745426.1 106636094 Pseudomonas fluorescens maoC NP 415905.1 16129348 Escherichia coli paaF NP 415911.1 16129354 Escherichia coli paaG NP 415912.1 16129355 Escherichia coli Alternatively, the E. coli gene products offadA andfadB encode a multienzyme complex involved in fatty acid oxidation that exhibits enoyl-CoA hydratase activity (Yang et al., Biochemistry 30:6788-6795 (1991); Yang, J Bacteriol. 173:7405-7406 (1991); 5 Nakahigashi et al., Nucleic Acids Res. 18:4937 (1990)). Knocking out a negative regulator encoded byfadR can be utilized to activate thefadB gene product (Sato et al., J Biosci.Bioeng 103:38-44 (2007)). ThefadI andfadJ genes encode similar functions and are naturally expressed under anaerobic conditions (Campbell et al., Mol.Microbiol 47:793-805 (2003)). Protein GenBank ID GI Number Organism fadA YP 026272.1 49176430 Escherichia coli fadB NP 418288.1 16131692 Escherichia coli fadI NP 416844.1 16130275 Escherichia coli fadl NP 416843.1 16130274 Escherichia coli fadR NP 415705.1 16129150 Escherichia coli 10 4.3.1.a Ammonia-Ivase In step J of Figure 1, an ammonia lyase in EC class 4.3.1 is required to catalyze the deamination of 5-aminopen-2-enoate to 2,4-pentadienoate. Exemplary enzymes are 15 aspartase and 3-methylaspartase. Aspartase (EC 4.3.1.1), catalyzing the deamination of aspartate to fumarate, has been characterized extensively (Viola, Adv Enzym Relat Areas Mol Biol, 74:295-341 (2000)). The E. coli enzyme is active on a variety of alternate substrates including aspartatephenylmethylester, asparagine, benzyl-aspartate and malate (Ma et al., Ann NY Acad Sci, 672:60-65 (1992)). In addition, directed evolution was 20 employed on this enzyme to alter substrate specificity (Asano et al., Biomol Eng 22:95 101 (2005)). The crystal structure of the E. coli aspartase, encoded by aspA, has been solved (Shi et al., Biochem, 36:9136-9144 (1997)). Enzymes with aspartase functionality have also been characterized in Haemophilus influenzae (Sjostrom et al., Biochim.Biophys.Acta 1324:182-190 (1997)), Pseudomonasfluorescens (Takagi et al., 25 J.Biochem. 96:545-552 (1984)), Bacillus subtilis (Sjostrom et al, Biochim Biophys Acta 1324:182-190 (1997)) and Serratia marcescens (Takagi et al., JBacteriol, 161:1-6 149 (1985)). 3-Methylaspartase catalyzes the deamination of threo-3-methylasparatate to mesaconate. The 3-methylaspartase from Clostridium tetanomorphum has been cloned, functionally expressed in E. coli, and crystallized (Asuncion et al., Acta Cryst D Biol Crystallog, 57:731-733 (2001); Asuncion et al., JBiol Chem. 277:8306-8311 (2002); 5 Botting et al., Biochem 27:2953-2955 (1988); Goda et al., Biochem 31:10747-10756 (1992)). In Citrobacter amalonaticus, this enzyme is encoded by BAA28 709 (Kato et al., Arch.Microbiol 168:457-463 (1997)). 3-methylaspartase has also been crystallized from E. coli YG1002 (Asano et al., FEMSMicrobiol Lett. 118:255-258 (1994)) although the protein sequence is not listed in public databases such as GenBank..Sequence homology 10 can be used to identify additional candidate genes, including CTC_02563 in C. tetani and ECsO761 in Escherichia coli 0157:H7. Protein GenBauk ID GI Number Origanism aspA NP 418562 90111690 Escherichia coli K12 subsp. MG1655 aspA P44324.1 1168534 Haemophilus influenzae aspA P07346.1 114273 Pseudomonasfluorescens ansB P26899.1 251757243 Bacillus subtilis aspA P33109.1 416661 Serratia marcescens MAL AAB24070.1 259429 Clostridium tetanomorphum BAA28709 BAA28709.1 3184397 Citrobacter amalonaticus CTC 02563 NP 783085.1 28212141 Clostridium tetani ECsO761 BAB34184.1 13360220 Escherichia coli 015 7:H7 str. Sakai 5,3.3.a Delta-isomerase 15 Several characterized enzymes shift the double bond of enoyl-CoA substrates from the 2 to the 3- position. Such a transformation is required in step D of Figure 3. Exemplary enzymes include 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta-isomerase (EC 5.3.3.3), delta-3, delta-2-enoyl-CoA isomerase (EC 5.3.3.8) and fatty acid oxidation complexes. 4-Hydroxybutyrul-CoA dehydratase enzymes catalyze the reversible 20 conversion of 4-hydroxybutyryl-CoA to crotonyl-CoA. These enzymes are bifunctional, catalyzing both the dehydration of 4-hydroxybutyryl-CoA to vinylacetyl-CoA, and also the isomerization of vinylacetyl-CoA and crotonyl-CoA. 4-Hydroxybutyrul-CoA dehydratase enzymes from C. aminobutyrium and C. kluyveri were purified, characterized, and sequenced at the N-terminus (Scherf et al., Arch.Microbiol 161:239-245 (1994); 25 Scherf and Buckel, Eur.JBiochem. 215:421-429 (1993)). The C. kluyveri enzyme, encoded by abJD, was cloned, sequenced and expressed in E. coli (Gerhardt et al., Arch.Microbiol 174:189-199 (2000)). The abfD gene product from Porphyromonas 150 gingivalis A TCC 33277 is closely related by sequence homology to the Clostridial gene products. 4-Hydroxybutyryl-CoA dehydratase/isomerase activity was also detected in Metallosphaera sedula, and is likely associated with the Msed 1220 gene (Berg et al, Science 318(5857):1782-6 (2007). Delta isomerization reactions are also catalyzed by the 5 fatty acid oxidation complex. In E. coli, thefadJ andfadB gene products convert cis-3 enoyl-CoA molecules to trans-2-enoyl-CoA molecules under aerobic and anaerobic conditions, respectively (Campbell et al, Mol Micro 47(3):793-805 (2003)). A monofunctional delta-isomerase isolated from Cucumis sativus peroxisomes catalyzes the reversible conversion of both cis- and trans-3-enoyl-CoA into trans-2-enoyl-CoA 10 (Engeland et al, Eur JBiochem, 196 (3):699-705 (1991). The gene associated with this enzyme has not been identified to date. A number of multifunctional proteins (MFP) from Cucumis sativus also catalyze this activity, including the gene product of MFP-a (Preisig Muller et al, JBiol Chem 269:20475-81 (1994)). Gene GenBank GI Number Organism abfD P55792 84028213 Clostridium aminobutyricum abfD YP 001396399.1 153955634 Clostridium kluyveri abfD YP_001928843 188994591 Porphyromonas gingivalis Msed 1220 ABP95381.1 145702239 Metallosphaera sedula fadJ AAC75401.1 1788682 Escherichia coli fadB AAC76849.1 1790281 Escherichia coli MFP-a Q39659.1 34922495 Cucumis sativus 15 6.2.1.a Acid-thiol ligase The conversion of acyl-CoA substrates to their acid products can be catalyzed by a CoA acid-thiol ligase or CoA synthetase in the 6.2.1 family of enzymes, several of which are reversible. Several reactions shown in Figures 1-6 are catalyzed by acid-thiol ligase 20 enzymes. These reactions include Steps L, P and 0 of Figure 1, step F of Figure 3, step B of Figure 4, step J of Figure 5 and step F of Figure 6. Several enzymes catalyzing CoA acid-thiol ligase or CoA synthetase activities have been described in the literature and represent suitable candidates for these steps. For example, ADP-forming acetyl-CoA synthetase (ACD, EC 6.2.1.13) is an enzyme that 25 couples the conversion of acyl-CoA esters to their corresponding acids with the concomitant synthesis of ATP. ACD I from Archaeoglobusfulgidus, encoded by AF1211, 151 was shown to operate on a variety of linear and branched-chain substrates including isobutyrate, isopentanoate, and fumarate (Musfeldt et al., JBacteriol. 184:636-644 (2002)). A second reversible ACD in Archaeoglobusfulgidus, encoded by AFI 983, was also shown to have a broad substrate range with high activity on cyclic compounds 5 phenylacetate and indoleacetate (Musfeldt and Schonheit, JBacteriol. 184:636-644 (2002)). The enzyme from Haloarcula marismortui (annotated as a succinyl-CoA synthetase) accepts propionate, butyrate, and branched-chain acids (isovalerate and isobutyrate) as substrates, and was shown to operate in the forward and reverse directions (Brasen et al., Arch Microbiol 182:277-287 (2004)). The ACD encoded by PAE3250 from 10 hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetyl-CoA, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen et al, supra). Directed evolution or engineering can be used to modify this enzyme to operate at the physiological temperature of the host organism. The enzymes from A. fulgidus, H. marismortui and P. aerophilum have all been 15 cloned, functionally expressed, and characterized in E. coli (Brasen and Schonheit, supra; Musfeldt and Schonheit, J Bacteriol. 184:636-644 (2002)). An additional candidate is succinyl-CoA synthetase, encoded by sucCD of E. coli and LSCJ and LSC2 genes of Saccharomyces cerevisiae. These enzymes catalyze the formation of succinyl-CoA from succinate with the concomitant consumption of one ATP in a reaction which is reversible 20 in vivo (Buck et al., Biochemistry 24:6245-6252 (1985)). The acyl CoA ligase from Pseudomonas putida has been demonstrated to work on several aliphatic substrates including acetic, propionic, butyric, valeric, hexanoic, heptanoic, and octanoic acids and on aromatic compounds such as phenylacetic and phenoxyacetic acids (Fernandez Valverde et al., Appl.Environ.Microbiol. 59:1149-1154 (1993)). A related enzyme, 25 malonyl CoA synthetase (6.3.4.9) from Rhizobium leguminosarum could convert several diacids, namely, ethyl-, propyl-, allyl-, isopropyl-, dimethyl-, cyclopropyl-, cyclopropylmethylene-, cyclobutyl-, and benzyl-malonate into their corresponding monothioesters (Pohl et al., J.Am.Chem.Soc. 123:5822-5823 (2001)). Protein GenBank ID GI Number Organism AF1211 NP 070039.1 11498810 Archaeoglobusfulgidus AF1983 NP 070807.1 11499565 Archaeoglobusfulgidus Scs YP 135572.1 55377722 Haloarcula marismortui PAE3250 NP_560604.1 18313937 Pyrobaculum aerophilum str. IM2 sucC NP 415256.1 16128703 Escherichia coli sucD AAC73823.1 1786949 Escherichia coli 152 Protein GenBank ID GI Number Organism LSCJ NP 014785 6324716 Saccharomyces cerevisiae LSC2 NP 011760 6321683 Saccharomyces cerevisiae paaF AAC24333.2 22711873 Pseudomonas putida matB AAC83455.1 3982573 Rhizobium leguminosarum Another candidate enzyme for these steps is 6-carboxyhexanoate-CoA ligase, also known as pimeloyl-CoA ligase (EC 6.2.1.14), which naturally activates pimelate to pimeloyl-CoA during biotin biosynthesis in gram-positive bacteria. The enzyme from Pseudomonas 5 mendocina, cloned into E. coli, was shown to accept the alternate substrates hexanedioate and nonanedioate (Binieda et al., Biochem.J 340 ( Pt 3):793-801 (1999)). Other candidates are found in Bacillus subtilis (Bower et al., JBacteriol. 178:4122-4130 (1996)) and Lysinibacillus sphaericus (formerly Bacillus sphaericus) (Ploux et al., Biochem.J 287 ( Pt 3):685-690 (1992)). Protein GenBank ID GI Number Organism bioW NP 390902.2 50812281 Bacillus subtilis bioW CAA10043.1 3850837 Pseudomonas mendocina bioW P22822.1 115012 Bacillus sphaericus 10 Additional CoA-ligases include the rat dicarboxylate-CoA ligase for which the sequence is yet uncharacterized (Vamecq et al., Biochem.J230:683-693 (1985)), either of the two characterized phenylacetate-CoA ligases from P. chrysogenum (Lamas-Maceiras et al., Biochem.J395:147-155 (2006); Wang et al., 360:453-458 (2007)), the phenylacetate-CoA 15 ligase from Pseudomonas putida (Martinez-Blanco et al., JBiol Chem 265:7084-7090 (1990)) and the 6-carboxyhexanoate-CoA ligase from Bacillus subtilis (Bower et al. J Bacteriol 178(14):4122-4130 (1996)). Acetoacetyl-CoA synthetases from Mus musculus (Hasegawa et al., Biochim Biophys Acta 1779:414-419 (2008)) and Homo sapiens (Ohgami et al., Biochem.Pharmacol. 65:989-994 (2003)) naturally catalyze the ATP 20 dependent conversion of acetoacetate into acetoacetyl-CoA. Protein Accession No. GI No. Organism phl CAJ15517.1 77019264 Penicillium chrysogenum phIB ABS19624.1 152002983 Penicillium chrysogenum paaF AAC24333.2 22711873 Pseudomonas putida bioW NP 390902.2 50812281 Bacillus subtilis AACS NP 084486.1 21313520 Mus musculus AACS NP 076417.2 31982927 Homo sapiens 153 Like enzymes in other classes, certain enzymes in the EC class 6.2.1 have been determined to have broad substrate specificity. The acyl CoA ligase from Pseudomonas putida has been demonstrated to work on several aliphatic substrates including acetic, propionic, butyric, valeric, hexanoic, heptanoic, and octanoic acids and on aromatic compounds such 5 as phenylacetic and phenoxyacetic acids (Femandez-Valverde et al., Applied and Environmental Microbiology 59:1149-1154 (1993)). A related enzyme, malonyl CoA synthetase (6.3.4.9) from Rhizobium triolii could convert several diacids, namely, ethyl-, propyl-, allyl-, isopropyl-, dimethyl-, cyclopropyl-, cyclopropylmethylene-, cyclobutyl-, and benzyl-malonate into their corresponding monothioesters (Pohl et al., J.Am.Chem.Soc. 10 123:5822-5823 (2001)). N/A (No EC number) In step Q of Figure 1, the conversion of 5-hydroxyvaleryl-CoA to 2,4-pentadienoyl-CoA is catalyzed by 5-hydroxyvaleryl-CoA dehydratase/dehydratase, a bifunctional enzyme. 15 Participating in 5-aminovalerate fermentation by Clostridium aminovalericum, this enzyme was purified characterized and crystallized (Eikmanns et al, Proteins: Struct Fun Gen 19:269-271 (1994), Eikmanns and Buckel, Eur JBiochem, 197:661-668 (1991)). The protein sequence is known but has not been assigned a GenBank identifier to date. Homologs with similar protein sequences are listed in the table below. Gene GenBank ID GI Number Organism CLOSS21 02963 ZP 02440459.1 167768406 Clostridium sp. SS2/1 CK3_30740 CBL42530.1 291563714 butyrate-producing bacterium SS3/4 ANACAC_0I,346 ZP_02418762.1 167746635 Anaerostipes caccae DSM 14662 mmgC2 ZP 07921990.1 315925783 Pseudoramibacter alactolyticus ANACAC 01346 ZP 07822451.1 167746635 Peptoniphilus harei FgonA2_010100002879 ZP_05630680.1 257466369 Fusobacterium gonidiaformans FNP_2146 ZP04969457.1 254302099 Fusobacterium nucleatum acdA2 ZP_07921487.1 315925275 Pseudoramibacter alactolyticus CHY_1732 YP_360552.1 78043883 Carboxydothermus h drogenoformans acdA ZP 07454495.1 306820875 Eubacterium yurii 20 154 Example VIII Chemical dehydration of 1,3-butanediol and 3-buten-1-ol to butadiene Alcohols can be converted to olefins by reaction with a suitable dehydration catalyst under appropriate conditions. Typical dehydration catalysts that convert alcohols such as 5 butanols and pentanols into olefins include various acid treated and untreated alumina (e.g., y-alumina) and silica catalysts and clays including zeolites (e.g., p-type zeolites, ZSM-5 or Y-type zeolites, fluoride-treated p-zeolite catalysts, fluoride-treated clay catalysts, etc.), sulfonic acid resins (e.g., sulfonated styrenic resins such as Amberlyst@ 15), strong acids such as phosphoric acid and sulfuric acid, Lewis acids such boron 10 trifluoride and aluminum trichloride, and many different types of metal salts including metal oxides (e.g., zirconium oxide or titanium dioxide) and metal chlorides (e.g., Latshaw B E, Dehydration of Isobutanol to Isobutylene in a Slurry Reactor, Department of Energy Topical Report, February 1994). Dehydration reactions can be carried out in both gas and liquid phases with both 15 heterogeneous and homogeneous catalyst systems in many different reactor configurations. Typically, the catalysts used are stable to the water that is generated by the reaction. The water is usually removed from the reaction zone with the product. The resulting alkene(s) either exit the reactor in the gas or liquid phase (e.g., depending upon the reactor conditions) and are captured by a downstream purification process or are 20 further converted in the reactor to other compounds (such as butadiene or isoprene) as described herein. The water generated by the dehydration reaction exits the reactor with unreacted alcohol and alkene product(s) and is separated by distillation or phase separation. Because water is generated in large quantities in the dehydration step, the dehydration catalysts used are generally tolerant to water and a process for removing the 25 water from substrate and product may be part of any process that contains a dehydration step. For this reason, it is possible to use wet (i.e., up to about 95% or 98% water by weight) alcohol as a substrate for a dehydration reaction and remove this water with the water generated by the dehydration reaction (e.g., using a zeolite catalyst as described U.S. Pat. Nos. 4,698,452 and 4,873,392). Additionally, neutral alumina and zeolites will 30 dehydrate alcohols to alkenes but generally at higher temperatures and pressures than the acidic versions of these catalysts. Dehydration of 1,3-butaediol to 3-buten-1-ol and butadiene is known in the art. For example, 3-buten-1-ol is synthesized from 1,3-butanediol by heating the diol in the 155 presence of a trivalent metal sulfate to a temperature in the range of 70-100 degrees Celcius (US Patent 4400562). The dehydration of 1,3-butanediol to butadiene entails, for example, heating 1,3-butanediol in the presence of superheated steam and a phosphate phosphoric acid catalyst (Sato, et al, Catalysis Communications, 5 (8), 2004, p. 397-400). 5 Dehydration of 3-buten- I -ol to butadiene is also well known in the art (Gustav. Egloff and George. Hulla, Chem. Rev., 1945, 36 (1), pp 63-141). EXAMPLE IX Exemplary Hydrogenase and CO Dehydrogenase Enzymes for Extracting Reducing Equivalents from Syngas and Exemplary Reductive TCA Cycle Enzymes 10 Enzymes of the reductive TCA cycle useful in the non-naturally occurring microbial organisms of the present invention include one or more of ATP-citrate lyase and three C0 2 -fixing enzymes: isocitrate dehydrogenase, alpha-ketoglutarate:ferredoxin oxidoreductase, pyruvate:ferredoxin oxidoreductase. The presence of ATP-citrate lyase or citrate lyase and alpha-ketoglutarate:ferredoxin oxidoreductase indicates the presence of 15 an active reductive TCA cycle in an organism. Enzymes for each step of the reductive TCA cycle are shown below. ATP-citrate lyase (ACL, EC 2.3.3.8), also called ATP citrate synthase, catalyzes the ATP dependent cleavage of citrate to oxaloacetate and acetyl-CoA. ACL is an enzyme of the RTCA cycle that has been studied in green sulfur bacteria Chlorobium limicola and 20 Chlorobium tepidum. The alpha(4)beta(4) heteromeric enzyme from Chlorobium limicola was cloned and characterized in E. coli (Kanao et al., Eur. J. Biochem. 269:3409-3416 (2002). The C. limicola enzyme, encoded by aclAB, is irreversible and activity of the enzyme is regulated by the ratio of ADP/ATP. A recombinant ACL from Chlorobium tepidum was also expressed in E. coli and the holoenzyme was reconstituted in vitro, in a 25 study elucidating the role of the alpha and beta subunits in the catalytic mechanism (Kim and Tabita, J. Bacteriol. 188:6544-6552 (2006). ACL enzymes have also been identified in Balnearium lithotrophicum, Sulfurihydrogenibium subterraneum and other members of the bacterial phylum Aquificae (Hugler et al., Environ. Microbiol. 9:81-92 (2007)). This acitivy has been reported in some fungi as well. Exemplary organisms include Sordaria 30 macrospora (Nowrousian et al., Curr. Genet. 37:189-93 (2000), Aspergillus nidulans , Yarrowia lipolytica (Hynes and Murray, Eukaryotic Cell, July: 1039-1048, (2010) and Aspergillus niger (Meijer et al. J. Ind. Microbiol. Biotechnol. 36:1275-1280 (2009). Other 156 candidates can be found based on sequence homology. Information related to these enzymes is tabulated below: Protein GenBank ID GI Number Organism aclA BAB21376.1 12407237 Chlorobium limicola aciB BAB21375.1 12407235 Chlorobium limicola acA AAM72321.1 21647054 Chlorobiumtepidum aciB AAM72322.1 21647055 Chlorobium tepidum aclA AB150076.1 114054981 Balnearium lithotrophicum aciB ABI50075.1 114054980 Balnearium lithotrophicum aclA ABI50085.1 114055040 Sulfurihydrogenibium subterraneum aclB AB150084.1 114055039 Sulfurihydrogenibium subterraneuin aclA AAX76834.1 62199504 Sulfurimonas denitrificans aciB AAX76835.1 62199506 Sulfurimonas denitrificans acil XP_504787.1 50554757 Yarrowia lipolytica ac! 2 XP_503231.1 50551515 Yarrowia lipolytica SPBC1 703.07 NP_596202.1 19112994 Schizosaccharomyces pombe SPAC22A12.16 NP_593246.1 19114158 Schizosaccharomycespombe acl1 CAB76165.1 7160185 Sordaria macrospora ac!2 CAB76164.1 7160184 Sordaria macrospora aclA CBF86850.1 259487849 Aspergillus nidulans aclB CBF86848 259487848 Aspergillus nidulans In some organisms the conversion of citrate to oxaloacetate and acetyl-CoA proceeds 5 through a citryl-CoA intermediate and is catalyzed by two separate enzymes, citryl-CoA synthetase (EC 6.2.1.18) and citryl-CoA lyase (EC 4.1.3.34) (Aoshima, M., AppL. Microbiol. Biotechnol. 75:249-255 (2007). Citryl-CoA synthetase catalyzes the activation of citrate to citryl-CoA. The Hydrogenobacter thermophilus enzyme is composed of large and small subunits encoded by ccsA and ccsB, respectively (Aoshima et al., MoL. Micrbiol. 10 52:751-761 (2004)). The citryl-CoA synthetase of Aquifex aeolicus is composed of alpha and beta subunits encoded by sucC1 and sucDl (Hugler et al., Environ. Microbiol. 9:81-92 (2007)). Citryl-CoA lyase splits citryl-CoA into oxaloacetate and acetyl-CoA. This enzyme is a homotrimer encoded by ccl in Hydrogenobacter thermophilus (Aoshima et al., MoL. Microbiol. 52:763-770 (2004)) and aq_150 in Aquifex aeolicus (Hugler et al., 157 supra (2007)). The genes for this mechanism of converting citrate to oxaloacetate and citryl-CoA have also been reported recently in Chlorobium tepidum (Eisen et al., PNAS 99(14): 9509-14 (2002). Protein GenBank ID GI Number Organism ccsA BAD17844.1 46849514 Hydrogenobacter thermophilus ccsB BAD17846.1 46849517 Hydrogenobacter thermophilus sucCi AAC07285 2983723 Aquifex aeolicus sucDl AAC07686 2984152 Aquifex aeolicus ccl BAD17841.1 46849510 Hydrogenobacter thermophilus aq_150 AAC06486 2982866 Aquifex aeolicus CT0380 NP_661284 21673219 Chlorobium tepidum CT0269 NP_661173.1 21673108 Chlorobium tepidum CT1834 AAM73055.1 21647851 Chlorobiumtepidum 5 Oxaloacetate is converted into malate by malate dehydrogenase (EC 1.1.1.37), an enzyme which functions in both the forward and reverse direction. S. cerevisiae possesses three copies of malate dehydrogenase, MDH1 (McAlister-Henn and Thompson, J. Bacteriol. 169:5157-5166 (1987), MDH2 (Minard and McAlister-Henn, Mol. Cell. Biol. 11:370-380 (1991); Gibson and McAlister-Henn, J. Biol. Chem. 278:25628-25636 (2003)), and MDH3 10 (Steffan and McAlister-Henn, J. Biol. Chem. 267:24708-24715 (1992)), which localize to the mitochondrion, cytosol, and peroxisome, respectively. E. coli is known to have an active malate dehydrogenase encoded by mdh. Protein GenBank ID GI Number Organism MDHI NP_012838 6322765 Saccharomyces cerevisiae MDH2 NP_014515 116006499 Saccharomyces cerevisiae MDH3 NP_010205 6320125 Saccharomyces cerevisiae Mdh NP_417703.1 16131126 Escherichia coli Fumarate hydratase (EC 4.2.1.2) catalyzes the reversible hydration of fumarate to malate. 15 The three fumarases of E. coli, encoded byfumA,fumB andfumC, are regulated under different conditions of oxygen availability. FumB is oxygen sensitive and is active under anaerobic conditions. FumA is active under microanaerobic conditions, and FumC is active under aerobic growth conditions (Tseng et al., J. Bacteriol. 183:461-467 158 (2001);Woods et al., Biochim. Biophys. Acta 954:14-26 (1988); Guest et al., J. Gen. Microbiol. 131:2971-2984 (1985)). S cerevisiae contains one copy of a fumarase encoding gene, FUMI, whose product localizes to both the cytosol and mitochondrion (Sass et al., J. Bio. Chem. 278:45109-45116 (2003)). Additional fumarase enzymes are 5 found in Campylobacterjejuni (Smith et al., Int. J. Biochem. Cell. Bio. 31:961-975 (1999)), Thermus thermophilus (Mizobata et al., Arch. Biochem. Biophys. 35 5:49-55 (1998)) and Rattus norvegicus (Kobayashi et al., J. Biochem. 89:1923-1931 (1981)). Similar enzymes with high sequence homology includefum1 from Arabidopsis thaliana and fumC from Corynebacterium glutamicum. The MmcBC fumarase from 10 Pelotomaculum thermopropionicum is another class of fumarase with two subunits (Shimoyama et al., FEMSMicrobiol. Lett. 270:207-213 (2007)). Protein GenBank ID GI Number Organism fumA NP_416129.1 16129570 Escherichia coli fumB NP_418546.1 16131948 Escherichia coli fumC NP_416128.1 16129569 Escherichia coli FUMI NP_015061 6324993 Saccharomyces cerevisiae fumC Q8NRN8.1 39931596 Corynebacterium glutamicum fumC 069294.1 9789756 Campylobacterjejuni fumC P84127 75427690 Thermus thermophilus fumH P14408.1 120605 Rattus norvegicus MmcB YP_001211906 147677691 Pelotomaculum thermopropionicum MmcC YP_001211907 147677692 Pelotomaculum thermopropionicum Fumarate reductase catalyzes the reduction of fumarate to succinate. The fumarate reductase of E. coli, composed of four subunits encoded byfrdABCD, is membrane-bound 15 and active under anaerobic conditions. The electron donor for this reaction is menaquinone and the two protons produced in this reaction do not contribute to the proton gradient (Iverson et al., Science 284:1961-1966 (1999)). The yeast genome encodes two soluble fumarate reductase isozymes encoded by FRDS1 (Enomoto et al., DNA Res. 3:263-267 (1996)) and FRDS2 (Muratsubaki et al., Arch. Biochem. Biophys. 352:175-181 20 (1998)), which localize to the cytosol and promitochondrion, respectively, and are used 159 during anaerobic growth on glucose (Arikawa et al., FEMS Microbiol. Lett. 165:111-116 (1998)). Protein GenBank ID GI Number Organism FRDS1 P32614 418423 Saccharomyces cerevisiae FRDS2 NP_012585 6322511 Saccharomyces cerevisiae frdA NP_418578.1 16131979 Escherichia coli frdB NP418577.1 16131978 Escherichia coli frdC NP_418576.1 16131977 Escherichia coli frdD NP_418475.1 16131877 Escherichia coli The ATP-dependent acylation of succinate to succinyl-CoA is catalyzed by succinyl-CoA 5 synthetase (EC 6.2.1.5). The product of the LSC1 and LSC2 genes of S. cerevisiae and the sucC and sucD genes of E. coli naturally form a succinyl-CoA synthetase complex that catalyzes the formation of succinyl-CoA from succinate with the concomitant consumption of one ATP, a reaction which is reversible in vivo (Buck et al., Biochemistry 24:6245-6252 (1985)). These proteins are identified below: Protein GenBank ID GI Number Organism LSC1 NP_014785 6324716 Saccharomyces cerevisiae LSC2 NP_011760 6321683 Saccharomyces cerevisiae sucC NP_415256.1 16128703 Escherichia coli sucD AAC73823.1 1786949 Escherichia coli 10 Alpha-ketoglutarate:ferredoxin oxidoreductase (EC 1.2.7.3), also known as 2-oxoglutarate synthase or 2-oxoglutarate:ferredoxin oxidoreductase (OFOR), forms alpha-ketoglutarate from C02 and succinyl-CoA with concurrent consumption of two reduced ferredoxin equivalents. OFOR and pyruvate:ferredoxin oxidoreductase (PFOR) are members of a 15 diverse family of 2-oxoacid:ferredoxin (flavodoxin) oxidoreductases which utilize thiamine pyrophosphate, CoA and iron-sulfur clusters as cofactors and ferredoxin, flavodoxin and FAD as electron carriers (Adams et al., Archaea. Adv. Protein Chem. 48:101-180 (1996)). Enzymes in this class are reversible and function in the carboxylation direction in organisms that fix carbon by the RTCA cycle such as 20 Hydrogenobacter thermophilus, Desulfobacter hydrogenophilus and Chlorobium species (Shiba et al. 1985; Evans et al., Proc. Natl. Acad. ScI. U.S.A. 55:92934 (1966); Buchanan, 160 1971). The two-subunit enzyme from H. thennophilus, encoded by korAB, has been cloned and expressed in E. coli (Yun et al., Biochem. Biophys. Res. Commun. 282:589 594 (2001)). A five subunit OFOR from the same organism with strict substrate specificity for succinyl-CoA, encoded byforDABGE, was recently identified and expressed in E. coli 5 (Yun et al. 2002). The kinetics of C02 fixation of both H. thermophilus OFOR enzymes have been characterized (Yamamoto et al., Extremophiles 14:79-85 (2010)). A C02-fixing OFOR from Chlorobium thiosulfatophilum has been purified and characterized but the genes encoding this enzyme have not been identified to date. Enzyme candidates in Chlorobium species can be inferred by sequence similarity to the H. thermophilus genes. 10 For example, the Chlorobium limicola genome encodes two similar proteins. Acetogenic bacteria such as Moorella thermoacetica are predicted to encode two OFOR enzymes. The enzyme encoded by Moth 0034 is predicted to function in the C02-assimilating direction. The genes associated with this enzyme, Moth_0034 have not been experimentally validated to date but can be inferred by sequence similarity to known OFOR enzymes. 15 OFOR enzymes that function in the decarboxylation direction under physiological conditions can also catalyze the reverse reaction. The OFOR from the thermoacidophilic archaeon Sulfolobus sp. strain 7, encoded by ST2300, has been extensively studied (Zhang et al. 1996. A plasmid-based expression system has been developed for efficiently expressing this protein in E. coli (Fukuda et al., Eur. J. Biochem. 268:5639-5646 (2001)) 20 and residues involved in substrate specificity were determined (Fukuda and Wakagi, Biochim. Biophys. Acta 1597:74-80 (2002)). The OFOR encoded by Ape1472/Ape1473 from Aeropyrum pernix str. KI was recently cloned into E. coli, characterized, and found to react with 2-oxoglutarate and a broad range of 2-oxoacids (Nishizawa et al., FEBS Lett. 579:2319-2322 (2005)). Another exemplary OFOR is encoded by oorDABC in 25 Helicobacter pylori (Hughes et al. 1998). An enzyme specific to alpha-ketoglutarate has been reported in Thauera aromatica (Dorner and Boll, J, Bacteriol. 184 (14), 3975-83 (2002). A similar enzyme can be found in Rhodospirillum rubrum by sequence homology. A two subunit enzyme has also been identified in Chlorobium tepidum (Eisen et al., PNAS 99(14): 9509-14 (2002)). Protein GenBank ID GI Number Organism korA BAB21494 12583691 Hydrogenobacter thermophilus korB BAB21495 12583692 Hydrogenobacter thermophilus forD BAB62132.1 14970994 Hydrogenobacter thermophilus 161 forA BAB62133.1 14970995 Hydrogenobacter thermophilus forB BAB62134.1 14970996 Hydrogenobacter thermophilus forG BAB62135.1 14970997 Hydrogenobacter thermophilus forE BAB62136.1 14970998 Hydrogenobacter thermophilus Clim_0204 ACD89303.1 189339900 Chlorobium limicola Clim_0205 ACD89302.1 189339899 Chlorobium limicola Clim_1123 ACD90192.1 189340789 Chlorobium limicola Clim_1124 ACD90193.1 189340790 Chlorobium limicola Moth_1984 YP_430825.1 83590816 Moorella thermoacetica Moth_1985 YP_430826.1 83590817 Moorella thermoacetica Moth_0034 YP_428917.1 83588908 Moorella thermoacetica ST2300 NP_378302.1 15922633 Sulfolobus sp. strain 7 Ape1472 BAA80470.1 5105156 Aeropyrum pernix Ape1473 BAA80471.2 116062794 Aeropyrum pernix oorD NP_207383.1 15645213 Helicobacterpylori oorA NP_207384.1 15645214 Helicobacterpylori oorB NP_207385.1 15645215 Helicobacterpylori oorC NP_207386.1 15645216 Helicobacterpylori CT0163 NP_661069.1 21673004 Chlorobium tepidum CT0162 NP_661068.1 21673003 Chlorobiumtepidum korA CAA12243.2 19571179 Thauera aromatica korB CAD27440.1 19571178 Thauera aromatica Rru_A 2721 YP_427805.1 83594053 Rhodospirillum rubrum RruA2722 YP_427806.1 83594054 Rhodospirillum rubrum Isocitrate dehydrogenase catalyzes the reversible decarboxylation of isocitrate to 2 oxoglutarate coupled to the reduction of NAD(P)+. IDH enzymes in Saccharomyces cerevisiae and Escherichia coli are encoded by IDP1 and icd, respectively (Haselbeck and 5 McAlister-Henn, J. Biol. Chem. 266:2339-2345 (1991); Nimmo, H.G., Biochem. J. 234:317-2332 (1986)). The reverse reaction in the reductive TCA cycle, the reductive carboxylation of 2-oxoglutarate to isocitrate, is favored by the NADPH-dependent C0 2

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162 fixing IDH from Chlorobium limicola and was functionally expressed in E. coli (Kanao et al., Eur. J. Biochem. 269:1926-1931 (2002)). A similar enzyme with 95% sequence identity is found in the C. tepidum genome in addititon to some other candidates listed below. Protein GenBank ID GI Number Organism lcd ACI84720.1 209772816 Escherichia coli IDP1 AAA34703.1 171749 Saccharomyces cerevisiae Idh BAC00856.1 21396513 Chlorobium limicola Icd AAM71597.1 21646271 Chlorobium tepidum icd NP_952516.1 39996565 Geobacter sulfurreducens icd YP_393560. 78777245 Sulfurimonas denitrificans 5 In H. thermophilus the reductive carboxylation of 2-oxoglutarate to isocitrate is catalyzed by two enzymes: 2-oxoglutarate carboxylase and oxalosuccinate reductase. 2 Oxoglutarate carboxylase (EC 6.4.1.7) catalyzes the ATP-dependent carboxylation of alpha-ketoglutarate to oxalosuccinate (Aoshima and Igarashi, Mo. Microbiol. 62:748-759 10 (2006)). This enzyme is a large complex composed of two subunits. Biotinylation of the large (A) subunit is required for enzyme function (Aoshima et al., Mo. Microbiol. 51:791 798 (2004)). Oxalosuccinate reductase (EC 1. 1.1.-) catalyzes the NAD-dependent conversion of oxalosuccinate to D-threo-isocitrate. The enzyme is a homodimer encoded by icd in H. thennophilus. The kinetic parameters of this enzyme indicate that the enzyme 15 only operates in the reductive carboxylation direction in vivo, in contrast to isocitrate dehydrogenase enzymes in other organisms (Aoshima and Igarashi, J. BacterioL. 190:2050-2055 (2008)). Based on sequence homology, gene candidates have also been found in Thiobacillus denitrficans and Thermocrinis albus. Protein GenBank ID GI Number Organism cfiA BAF34932.1 116234991 Hydrogenobacter thermophilus cifB BAF34931.1 116234990 Hydrogenobacter thermophilus Icd BAD02487.1 38602676 Hydrogenobacter thermophilus Tbd_1556 YP_315314 74317574 Thiobacillus denitrificans Tbd_1555 YP_315313 74317573 Thiobacillus denitrificans Tbd_0854 YP_314612 74316872 Thiobacillusdenitrificans Thal_0268 YP_003473030 289548042 Thermocrinis albus 163 Protein GenBank ID GI Number Organism Thal_0267 YP_003473029 289548041 Thermocrinis albus Thal_0646 YP_003473406 289548418 Thermocrinis albus Aconitase (EC 4.2.1.3) is an iron-sulfur-containing protein catalyzing the reversible isomerization of citrate and iso-citrate via the intermediate cis-aconitate. Two aconitase enzymes are encoded in the E. coli genome by acnA and acnB. AcnB is the main catabolic 5 enzyme, while AcnA is more stable and appears to be active under conditions of oxidative or acid stress (Cunningham et al., Microbiology 143 (Pt 12):3795-3805 (1997)). Two isozymes of aconitase in Salmonella typhimurium are encoded by acn.A and acnB (Horswill and Escalante-Semerena, Biochemistry 40:4703-4713 (2001)). The S. cerevisiae aconitase, encoded by ACO, is localized to the mitochondria where it participates in the 10 TCA cycle (Gangloff et al., Mol. Cell. Biol. 10:3551-3561 (1990)) and the cytosol where it participates in the glyoxylate shunt (Regev-Rudzki et al., Mol. Biol. Cell. 16:4163-4171 (2005)). Protein GenBank ID GI Number Organism acnA AAC7438,1 1787531 Escherichia coli acnB AAC73229.1 2367097 Escherichia coli acnA NP 46067 1.1 16765056 Salmonella typhimurium HP0779 NP_207572.1 15645398 Helicobacterpylori 26695 H16_B0568 CAJ95365.1 113529018 Ralstonia eutropha DesfrDRAFT 3783 ZP_07335307.1 303249064 Desulfovibrio fructosovorans JJ Suden_1040 ABB44318.1 78497778 Sulfurimonas (acnB) denitrificans Hydth_0755 ADO45152.1 308751669 Hydrogenobacter thermophilus CT0543 (acn) AAM71785.1 21646475 Chlorobium tepidum Clim_2436 YP_001944436.1 189347907 Chlorobium limicola Clim 0515 ACD89607.1 189340204 Chlorobium limicola acnB NP_459163.1 16763548 Salmonella typhimurium ACO! AAA34389.1 170982 Saccharomyces cerevisiae Pyruvate:ferredoxin oxidoreductase (PFOR) catalyzes the reversible oxidation of pyruvate to form acetyl-CoA. The PFOR from Desulfovibrio africanus has been cloned and 15 expressed in E. coli resulting in an active recombinant enzyme that was stable for several 164 days in the presence of oxygen (Pieulle et al., J. Bacteriol. 179:5684-5692 (1997)). Oxygen stability is relatively uncommon in PFORs and is believed to be conferred by a 60 residue extension in the polypeptide chain of the D. africanus enzyme. Two cysteine residues in this enzyme form a disulfide bond that protects it against inactivation in the 5 form of oxygen. This disulfide bond and the stability in the presence of oxygen has been found in other Desulfovibrio species also (Vita et al., Biochemistry, 47: 957-64 (2008)). The M. thermoacetica PFOR is also well characterized (Menon and Ragsdale, Biochemistry 36:8484-8494 (1997)) and was shown to have high activity in the direction of pyruvate synthesis during autotrophic growth (Furdui and Ragsdale, J. Biol. Chem. 10 275:28494-28499 (2000)). Further, E. coli possesses an uncharacterized open reading frame, ydbK, encoding a protein that is 51% identical to the M. thermoacetica PFOR. Evidence for pyruvate oxidoreductase activity in E. coli has been described (Blaschkowski et al., Eur. J. Biochem. 123:563-569 (1982)), PFORs have also been described in other organisms, including Rhodobacter capsulatas (Yakunin and Hallenbeck, Biochimica et 15 Biophysica Acta 1409 (1998) 39-49 (1998)) and Choloboum tepidum (Eisen et al., PNAS 99(14): 9509-14 (2002)). The five subunit PFOR from H. thermophilus, encoded by porEDABG, was cloned into E. coli and shown to function in both the decarboxylating and C0 2 -assimilating directions (Ikeda et al. 2006; Yamamoto et al., Extremophiles 14:79-85 (2010)). Homologs also exist in C. carboxidivorans P7. Several additional PFOR 20 enzymes are described in the following review (Ragsdale, S.W., Chem. Rev. 103:2333 2346 (2003)). Finally, flavodoxin reductases (e.g.,fqrB from Helicobacterpylori or Campylobacterjejuni) (St Maurice et al., J. Bacteriol. 189:4764-4773 (2007)) or Rnf-type proteins (Seedorf et al., Proc. Nati. Acad. Sci. U.S.A. 105:2128-2133 (2008); and Herrmann, J. Bacteriol 190:784-791 (2008)) provide a means to generate NADH or 25 NADPH from the reduced ferredoxin generated by PFOR. These proteins are identified below. Protein GenBank ID GI Number Organism DesfrDRAFT_0121 ZP_07331646.1 303245362 Desulfovibrio fructosovorans JJ Por CAA70873.1 1770208 Desulfovibrio africanus por YP_012236.1 46581428 Desulfovibrio vulgaris str. Hildenborough Dde_3237 ABB4003 1.1 78220682 Desulfo Vibrio 1 -7 1 desulfuricans G20 165 Protein GenBank ID GI Number Organism Ddes_0298 YP_002478891.1 220903579 Desulfovibrio desulfuricans subsp. desulfuricans str. A TCC 27774 Por YP_428946.1 83588937 Moorella thermoacetica YdbK NP_415896.1 16129339 Escherichia coli nifJ (CT1628) NP_662511.1 21674446 Chlorobium tepidum CJE1649 YP179630.1 57238499 Campylobacterjejuni nifJ ADE85473.1 294476085 Rhodobacter capsulatus porE BAA95603.1 7768912 Hydrogenobacter thermnophilus porD BAA95604.1 7768913 Hydrogenobacter thennophilus porA BAA95605.1 7768914 Hydrogenobacter thermophilus porB BAA95606.1 776891 Hydrogenobacter thermophilus porG BAA95607.1 7768916 Hydrogenobacter thermnophilus FqrB YP_001482096.1 157414840 Campylobacterjejuni HP 1164 NP_207955.1 15645778 Helicobacterpylori RnfC EDK33306.1 146346770 Clostridium kluyveri RnfD EDK33307.1 146346771 Clostridium kluyveri RnfG EDK33308.1 146346772 Clostridium kluyveri RnfE EDK33309.1 146346773 Clostridium kluyveri RnfA EDK33310.1 146346774 Clostridium kluyveri RnfB EDK333 11.1 146346775 Clostridium kluyveri The conversion of pyruvate into acetyl-CoA can be catalyzed by several other enzymes or their combinations thereof For example, pyruvate dehydrogenase can transform pyruvate into acetyl-CoA with the concomitant reduction of a molecule of NAD into NADH. It is a 166 multi-enzyme complex that catalyzes a series of partial reactions which results in acylating oxidative decarboxylation of pyruvate. The enzyme comprises of three subunits: the pyruvate decarboxylase (El), dihydrolipoamide acyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). This enzyme is naturally present in several 5 organisms, including E. coli and S. cerevisiae. In the E. coli enzyme, specific residues in the El component are responsible for substrate specificity (Bisswanger, H., J. Biol. Chem. 256:815-82 (1981); Bremer, J., Eur. J. Biochem. 8:535-540 (1969); Gong et al., J. Biol. Chem. 275:13645-13653 (2000)). Enzyme engineering efforts have improved the E. coli PDH enzyme activity under anaerobic conditions (Kim et al., J. Bacteriol. 190:3851-3858 10 (2008); Kim et al., Appl. Environ. Microbiol. 73:1766-1771 (2007); Zhou et al., Biotechnol. Lett. 30:335-342 (2008)). In contrast to the E. coli PDH, the B. subtilis complex is active and required for growth under anaerobic conditions (Nakano et al., J. Bacteriol, 179:6749-6755 (1997)). The Klebsiella pneumoniae PDH, characterized during growth on glycerol, is also active under anaerobic conditions (5). Crystal structures of the 15 enzyme complex from bovine kidney (18) and the E2 catalytic domain from Azotobacter vinelandii are available (4). Yet another enzyme that can catalyze this conversion is pyruvate formate lyase. This enzyme catalyzes the conversion of pyruvate and CoA into acetyl-CoA and formate. Pyruvate formate lyase is a common enzyme in prokaryotic organisms that is used to help modulate anaerobic redox balance. Exemplary enzymes can 20 be found in Escherichia coli encoded by pflB (Knappe and Sawers, FEMS.Microbiol Rev. 6:383-398 (1990)), Lactococcus lactis (Melchiorsen et al., Appl Microbiol Biotechnol 58:338-344 (2002)), and Streptococcus mutans (Takahashi-Abbe et al., Oral.Microbiol Immunol. 18:293-297 (2003)). E. coli possesses an additional pyruvate formate lyase, encoded by tdcE, that catalyzes the conversion of pyruvate or 2-oxobutanoate to acetyl 25 CoA or propionyl-CoA, respectively (Hesslinger et al., Mol. Microbiol 27:477-492 (1998)). BothpflB and tdcE from E. coli require the presence of pyruvate formate lyase activating enzyme, encoded by pflA. Further, a short protein encoded by yfiD in E. coli can associate with and restore activity to oxygen-cleaved pyruvate formate lyase (Vey et al., Proc.Natl. Acad. S.ci. U.S.A. 105:16137-16141 (2008). Note that pflA and pflB from 30 E. coli were expressed in S. cerevisiae as a means to increase cytosolic acetyl-CoA for butanol production as described in WO/2008/080124]. Additional pyruvate formate lyase and activating enzyme candidates, encoded by pfl and act, respectively, are found in Clostridium pasteurianum (Weidner et al., J Bacteriol. 178:2440-2444 (1996)).

167 Further, different enzymes can be used in combination to convert pyruvate into acetyl CoA. For example, in S. cerevisiae, acetyl-CoA is obtained in the cytosol by first decarboxylating pyruvate to form acetaldehyde; the latter is oxidized to acetate by acetaldehyde dehydrogenase and subsequently activated to form acetyl-CoA by acetyl 5 CoA synthetase. Acetyl-CoA synthetase is a native enzyme in several other organisms including E. coli (Kumari et al., J. Bacteriol. 177:2878-2886 (1995)), Salmonella enterica (Starai et al., Microbiology 151:3793-3801 (2005); Starai et al., J. Biol. Chem. 280:26200 26205 (2005)), and Moorella thermoacetica (described already). Alternatively, acetate can be activated to form acetyl-CoA by acetate kinase and phosphotransacetylase. Acetate 10 kinase first converts acetate into acetyl-phosphate with the accompanying use of an ATP molecule. Acetyl-phosphate and CoA are next converted into acetyl-CoA with the release of one phosphate by phosphotransacetylase. Both acetate kinase and phosphotransacetlyase are well-studied enzymes in several Clostridia and Methanosarcina thermophila. 15 Yet another way of converting pyruvate to acetyl-CoA is via pyruvate oxidase. Pyruvate oxidase converts pyruvate into acetate, using ubiquione as the electron acceptor. In E. coli, this activity is encoded by poxB. PoxB has similarity to pyruvate decarboxylase of S. cerevisiae and Zymomonas mobilis. The enzyme has a thiamin pyrophosphate cofactor (Koland and Gennis, Biochemistry 21:4438-4442 (1982)); O'Brien et al., Biochemistry 20 16:3105-3109 (1977); O'Brien and Gennis, J. Biol. Chem. 255:3302-3307 (1980)) and a flavin adenine dinucleotide (FAD) cofactor. Acetate can then be converted into acetyl CoA by either acetyl-CoA synthetase or by acetate kinase and phosphotransacetylase, as described earlier. Some of these enzymes can also catalyze the reverse reaction from acetyl-CoA to pyruvate. 25 For enzymes that use reducing equivalents in the form of NADH or NADPH, these reduced carriers can be generated by transferring electrons from reduced ferredoxin. Two enzymes catalyze the reversible transfer of electrons from reduced ferredoxins to NAD(P)*, ferredoxin:NAD* oxidoreductase (EC 1.18.1.3) and ferredoxin:NADP+ oxidoreductase (FNR, EC 1.18.1.2). Ferredoxin:NADP* oxidoreductase (FNR, EC 30 1.18.1.2) has a noncovalently bound FAD cofactor that facilitates the reversible transfer of electrons from NADPH to low-potential acceptors such as ferredoxins or flavodoxins (Blaschkowski et al., Eur. J. Biochem. 123:563-569 (1982); Fujii et al., 1977). The Helicobacter pylori FNR, encoded by HP1164 (fqrB), is coupled to the activity of 168 pyruvate:ferredoxin oxidoreductase (PFOR) resulting in the pyruvate-dependent production of NADPH (St et al. 2007). An analogous enzyme is found in Campylobacter jejuni (St et al. 2007). A ferredoxin:NADP t oxidoreductase enzyme is encoded in the E. coli genome byfpr (Bianchi et al. 1993). Ferredoxin:NAD* oxidoreductase utilizes 5 reduced ferredoxin to generate NADH from NAD t . In several organisms, including E. coli, this enzyme is a component of multifunctional dioxygenase enzyme complexes. The ferredoxin:NAD* oxidoreductase of E. coli, encoded by hcaD, is a component of the 3 phenylproppionate dioxygenase system involved in involved in aromatic acid utilization (Diaz et al. 1998). NADH:ferredoxin reductase activity was detected in cell extracts of 10 Hydrogenobacter thermophilus strain TK-6, although a gene with this activity has not yet been indicated (Yoon et al. 2006). Finally, the energy-conserving membrane-associated Rnf-type proteins (Seedorf et al., Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133 (2008); Herrmann et al., J. Bacteriol. 190:784-791 (2008)) provide a means to generate NADH or NADPH from reduced ferredoxin. Additional ferredoxin:NAD(P)+ oxidoreductases have 15 been annotated in Clostridium carboxydivorans P7. Protein GenBank ID GI Number Organism HP1164 NP_207955.1 15645778 Helicobacterpylori RPA3954 CAE29395.1 39650872 Rhodopseudomonas palustris fpr BAH29712.1 225320633 Hydrogenobacter thermophilus yumC NP_391091.2 255767736 Bacillus subtilis CJE0663 AAW35824.1 57167045 Campylobacterjejuni fpr P28861.4 399486 Escherichia coli hcaD AAC75595.1 1788892 Escherichia coli LOC100282643 NP_001149023.1 226497434 Zea nays RnJC EDK33306.1 146346770 Clostridiwn kluyveri RnfD EDK33307.1 146346771 Clostridium kluyveri RnfG EDK33308.1 146346772 Clostridium kluyveri RnJE EDK33309.1 146346773 Clostridium kluyveri RnfA EDK33310.1 146346774 Clostridium kluyveri RnfB EDK3331 1.1 146346775 Clostridium kluyveri CcarbDRAFT_2639 ZP_05392639.1 255525707 Clostridium carboxidivorans P7 CcarbDRAFT_2638 ZP_05392638.1 255525706 Clostridium carboxidivorans P7 169 7 CcarbDRAFT_2636 ZP_05392636.1 255525704 Clostridium carboxidivorans P7 CcarbDRAFT_5060 ZP_05395060.1 255528241 Clostridium carboxidivorans P7 CcarbDRAFT_2450 ZP_05392450.1 255525514 Clostridium I I_ carboxidivorans P7 CcarbDRAFT_1084 ZP_05391084.1 255524124 Clostridium carboxidivorans P7 Ferredoxins are small acidic proteins containing one or more iron-sulfur clusters that function as intracellular electron carriers with a low reduction potential. Reduced ferredoxins donate electrons to Fe-dependent enzymes such as ferredoxin-NADP* 5 oxidoreductase, pyruvate:ferredoxin oxidoreductase (PFOR) and 2 oxoglutarate:ferredoxin oxidoreductase (OFOR). The H. thermophilus genefdxl encodes a [4Fe-4S]-type ferredoxin that is required for the reversible carboxylation of 2 oxoglutarate and pyruvate by OFOR and PFOR, respectively (Yamamoto et al., Extremophiles 14:79-85 (2010)). The ferredoxin associated with the Sulfolobus 10 solfataricus 2-oxoacid:ferredoxin reductase is a monomeric dicluster [3Fe-4S][4Fe-4S] type ferredoxin (Park et al. 2006). While the gene associated with this protein has not been fully sequenced, the N-terminal domain shares 93% homology with the zfx ferredoxin from S. acidocaldarius. The E. coli genome encodes a soluble ferredoxin of unknown physiological function,fdx. Some evidence indicates that this protein can 15 function in iron-sulfur cluster assembly (Takahashi and Nakamura, 1999). Additional ferredoxin proteins have been characterized in Helicobacterpylori (Mukhopadhyay et al. 2003) and Campylobacterjejuni (van Vliet et al. 2001). A 2Fe-2S ferredoxin from Clostridium pasteurianum has been cloned and expressed in E. coli (Fujinaga and Meyer, Biochemical and Biophysical Research Communications, 192(3): (1993)). Acetogenic 20 bacteria such as Moorella thermoacetica, Clostridium carboxidivorans P7 and Rhodospirillum rubrum are predicted to encode several ferredoxins, listed in the table below. Protein GenBank ID GI Number Or2anism fdxl BAE02673.1 68163284 Hydrogenobacter the rmophilus M 11214.1 AAA83524.1 144806 Clostridium pasteurianum Zfx AAY79867.1 68566938 Sulfolobus acidocalarius Fdx AAC75578.1 1788874 Escherichia coli hp 0277 AAD07340.1 2313367 Helicobacter pylori 170 fdxA CAL34484.1 112359698 Campylobacterjiejuni Moth 0061 ABC18400.1 83571848 Moorella thermoacetica Moth 1200 ABC19514.1 83572962 Moorella thermoacetica Moth 1888 ABC20188.1 83573636 Moorella thermoacetica Moth 2112 ABC20404.1 83573852 Moorella thermoacetica Moth_1037 ABC19351.1 83572799 Moorella thermoacetica CcarbDRAFT_4383 ZP_05394383.1 255527515 Clostridium carboxidivorans P7 CcarbDRAFT_2958 ZP_05392958.1 255526034 Clostridium carboxidivorans P7 CcarbDRAFT_2281 ZP_05392281.1 255525342 Clostridium carboxidivorans P7 CcarbDRAFT_5296 ZP_05395295.1 255528511 Clostridium carboxidivorans P7 CcarbDRAFT_1615 ZP_05391615.1 255524662 Clostridium carboxidivorans P7 CcarbDRAFT_1304 ZP_05391304.1 255524347 Clostridium carboxidivorans P7 cooF AAG29808.1 11095245 Carboxydothermus hydrogenoformans fdxN CAA35699.1 46143 Rhodobacter capsulatus Rru A2264 ABC23064.1 83576513 Rhodospirillum rubrum Rru A1916 ABC22716.1 83576165 Rhodospirillum rubrum Rru A2026 ABC22826.1 83576275 Rhodospirillum rubrum cooF AAC45122.1 1498747 Rhodospirillum rubrum fdxN AAA26460.1 152605 Rhodospirillum rubrum Alvin_2884 ADC63789.1 288897953 Allochromatium vinosum DSM 180 fdx YP_002801146.1 226946073 Azotobacter vinelandii DJ CKL_3790 YP_001397146.1 153956381 Clostridium kluyveri DSM ___ ___ ___ ___ _ _ ___ ___ ___ 555 ferI NP_949965.1 39937689 Rhodopseudomonas palustris CGA009 fdx CAA12251.1 3724172 Thauera aromaica CHY_2405 YP_361202.1 78044690 Carboxydothermus hydrogenoformans fer YP_359966.1 78045103 Carboxydothermus hydrogenoformans fer AAC83945.1 1146198 Bacillus subtilis fdx1 NP_249053.1 15595559 Pseudomonas aeruginosa PA 01 yfhL AP 003148.1 89109368 Escherichia coliK-12 Succinyl-CoA transferase catalyzes the conversion of succinyl-CoA to succinate while transferring the CoA moiety to a CoA acceptor molecule. Many transferases have broad specificity and can utilize CoA acceptors as diverse as acetate, succinate, propionate, 171 butyrate, 2-methylacetoacetate, 3-ketohexanoate, 3-ketopentanoate, valerate, crotonate, 3 mercaptopropionate, propionate, vinylacetate, and butyrate, among others. The conversion of succinate to succinyl-CoA can be carried by a transferase which does not require the direct consumption of an ATP or GTP. This type of reaction is common in 5 a number of organisms. The conversion of succinate to succinyl-CoA can also be catalyzed by succinyl-CoA:Acetyl-CoA transferase. The gene product of cat1 of Clostridium kluyveri has been shown to exhibit succinyl-CoA: acetyl-CoA transferase activity (Sohling and Gottschalk, J. Bacteriol. 178:871-880 (1996)). In addition, the activity is present in Trichomonas vaginalis (van Grinsven et al. 2008) and Trypanosoma 10 brucei (Riviere et al. 2004). The succinyl-CoA:acetate CoA-transferase from Acetobacter aceti, encoded by aarC, replaces succinyl-CoA synthetase in a variant TCA cycle (Mullins et al. 2008). Similar succinyl-CoA transferase activities are also present in Trichomonas vaginalis (van Grinsven et al. 2008), Trypanosoma brucei (Riviere et al. 2004) and Clostridium kluyveri (Sohling and Gottschalk, 1996c). The beta-ketoadipate:succinyl 15 CoA transferase encoded by pcaI and pcaJ in Pseudomonas putida is yet another candidate (Kaschabek et al. 2002). The aforementioned proteins are identified below. Protein GenBank ID GI Number Organism cat] P38946.1 729048 Clostridium kluyveri TVAG_395550 XP_001330176 123975034 Trichomonas vaginalis G3 Th1.02.0290 XP_828352 71754875 Trypanosoma brucei pcaI AAN69545.1 24985644 Pseudomonas putida pcaJ NP_746082.1 26990657 Pseudomonas putida aarC ACD85596.1 189233555 Acetobacter aceti An additional exemplary transferase that converts succinate to succinyl-CoA while converting a 3-ketoacyl-CoA to a 3-ketoacid is succinyl-CoA:3:ketoacid-CoA transferase 20 (EC 2.8.3.5). Exemplary succinyl-CoA:3:ketoacid-CoA transferases are present in Helicobacterpylori (Corthesy-Theulaz et al. 1997), Bacillus subtilis, and Homo sapiens (Fukao et al. 2000; Tanaka et al. 2002). The aforementioned proteins are identified below. Protein GenBank ID GI Number Organism HPAGJ_0676 YP_627417 108563101 Helicobacterpylori HPAGJ_0677 YP_627418 108563102 Helicobacterpylori 172 Protein GenBank ID GI Number Organism ScoA NP_391778 16080950 Bacillus subtilis ScoB NP_391777 16080949 Bacillus subtilis OXCTJ NP_000427 4557817 Homo sapiens OXCT2 NP_071403 11545841 Homo sapiens Converting succinate to succinyl-CoA by succinyl-CoA:3:ketoacid-CoA transferase requires the simultaneous conversion of a 3-ketoacyl-CoA such as acetoacetyl-CoA to a 3 ketoacid such as acetoacetate. Conversion of a 3-ketoacid back to a 3-ketoacyl-CoA can 5 be catalyzed by an acetoacetyl-CoA:acetate:CoA transferase. Acetoacetyl CoA:acetate:CoA transferase converts acetoacetyl-CoA and acetate to acetoacetate and acetyl-CoA, or vice versa. Exemplary enzymes include the gene products of atoAD from E. coli (Hanai et al., Appi Environ Microbiol 73:7814-7818 (2007), ctfAB from C. acetobutylicum (Jojima et al., Appi Microbiol Biotechnol 77:1219-1224 (2008), and ctfAB 10 from Clostridium saccharoperbutylacetonicum (Kosaka et al., Biosci.Biotechnol Biochem. 71:58-68 (2007)) are shown below. Protein GenBank ID GI Number Organism AtoA NP_416726.1 2492994 Escherichia coli AtoD NP_416725.1 2492990 Escherichia coli CtfA NP_149326.1 15004866 Clostridium acetobutylicum CtfB NP_149327.1 15004867 Clostridium acetobutylicum CtfA AAP42564.1 31075384 Clostridium saccharoperbutylacetonicum C fB AAP42565.1 31075385 Clostridium saccharoperbutylacetonicum Yet another possible CoA acceptor is benzylsuccinate. Succinyl-CoA:(R) Benzylsuccinate CoA-Transferase functions as part of an anaerobic degradation pathway 15 for toluene in organisms such as Thauera aromatica (Leutwein and Heider, J. Bact. 183(14) 4288-4295 (2001)). Homologs can be found in Azoarcus sp. T, Aromatoleum aromaticum EbN 1, and Geobacter metallireducens GS- 15. The aforementioned proteins are identified below. Protein GenBank ID GI Number Organism bbsE AAF89840 9622535 Thauera aromatic Bbsf. AAF89841 9622536 Thauera aromatic 173 Protein GenBank ID GI Number Organism bbsE AAU45405.1 52421824 Azoarcus sp. T bbsF AAU45406.1 52421825 Azoarcus sp. T bbsE YP_158075.1 56476486 Aromatoleum aromaticum EbNJ bbsF YP_158074.1 56476485 Aromatoleum aromaticum EbNJ Gmet_1521 YP_384480.1 78222733 Geobacter metallireducens GS-15 Gmet_1522 YP_384481.1 78222734 Geobacter metallireducens GS-15 Additionally, ygfH encodes a propionyl CoA:succinate CoA transferase in E. coli (Haller et al., Biochemistry, 39(16) 4622-4629). Close homologs can be found in, for example, Citrobacter youngae ATCC 29220, Salmonella enterica subsp. arizonae serovar, and 5 Yersinia intermedia ATCC 29909. The aforementioned proteins are identified below. Protein GenBank ID GI Number Organism ygfH NP_417395.1 16130821 Escherichia coli str. K-12 substr. MG1655 CIT292_04485 ZP_03838384.1 227334728 Citrobacter youngae A TCC 29220 SARI_04582 YP_001573497.1 161506385 Salmonella enterica subsp. arizonae serovar yinte000l_14430 ZP_04635364.1 238791727 Yersinia intermedia ATCC 29909 Citrate lyase (EC 4.1.3.6) catalyzes a series of reactions resulting in the cleavage of citrate to acetate and oxaloacetate. The enzyme is active under anaerobic conditions and is composed of three subunits: an acyl-carrier protein (ACP, gamma), an ACP transferase 10 (alpha), and a acyl lyase (beta). Enzyme activation uses covalent binding and acetylation of an unusual prosthetic group, 2'-(5"-phosphoribosyl)-3-'-dephospho-CoA, which is similar in structure to acetyl-CoA. Acylation is catalyzed by CitC, a citrate lyase synthetase. Two additional proteins, CitG and CitX, are used to convert the apo enzyme into the active holo enzyme (Schneider et al., Biochemistry 39:9438-9450 (2000)). Wild 15 type E. coli does not have citrate lyase activity; however, mutants deficient in molybdenum cofactor synthesis have an active citrate lyase (Clark, FEMSMicrobiol. Lett. 55:245-249 (1990)). The E. coli enzyme is encoded by citEFD and the citrate lyase 174 synthetase is encoded by citC (Nilekani and SivaRaman, Biochemistry 22:4657-4663 (1983)). The Leuconostoc mesenteroides citrate lyase has been cloned, characterized and expressed in E. coli (Bekal et al., J. Bacteriol. 180:647-654 (1998)). Citrate lyase enzymes have also been identified in enterobacteria that utilize citrate as a carbon and 5 energy source, including Salmonella typhimurium and Klebsiella pneumoniae (Bott, Arch. Microbiol. 167: 78-88 (1997); Bott and Dimroth, Mol. Microbiol. 14:347-356 (1994)). The aforementioned proteins are tabulated below. Protein GenBank ID GI Number Ornanism citF AAC73716.1 1786832 Escherichia coli Cite AAC73717.2 87081764 Escherichia coli citD AAC73718.1 1786834 Escherichia coli citC AAC73719.2 87081765 Escherichia coli citG AAC73714.1 1786830 Escherichia coli citX AAC73715.1 1786831 Escherichia coli citF CAA71633.1 2842397 Leuconostoc mesenteroides Cite CAA71632.1 2842396 Leuconostoc mesenteroides citD CAA71635.1 2842395 Leuconostoc mesenteroides citC CAA71636.1 3413797 Leuconostoc mesenteroides citG CAA71634.1 2842398 Leuconostoc mesenteroides citX CAA71634.1 2842398 Leuconostoc mesenteroides citF NP_459613.1 16763998 Salmonella typhimurium cite AAL19573.1 16419133 Salmonella typhimurium citD NP_459064.1 16763449 Salmonella typhimurium citC NP_459616.1 16764001 Salmonella typhimurium citG NP_459611.1 16763996 Salmonella typhimurium citX NP_459612.1 16763997 Salmonella typhimurium citF CAA56217.1 565619 Klebsiella pneumoniae 175 Protein GenBank ID GI Number Organism cite CAA56216.1 565618 Klebsiella pneumoniae citD CAA56215.1 565617 Klebsiella pneumoniae citC BAH66541.1 238774045 Klebsiella pneumoniae citG CAA56218.1 565620 Klebsiella pneumoniae citX AAL60463.1 18140907 Klebsiella pneumoniae Acetate kinase (EC 2.7.2.1) catalyzes the reversible ATP-dependent phosphorylation of acetate to acetylphosphate. Exemplary acetate kinase enzymes have been characterized in many organisms including E. coli, Clostridium acetobutylicum and Methanosarcina 5 thermophila (Ingram-Smith et al., J. Bacteriol. 187:2386-2394 (2005); Fox and Roseman, J. Biol. Chem. 261:13487-13497 (1986); Winzer et al., Microbioloy 143 (Pt 10):3279 3286 (1997)). Acetate kinase activity has also been demonstrated in the gene product of E. colipurT(Marolewski et al., Biochemistry 33:2531-2537 (1994). Some butyrate kinase enzymes (EC 2.7.2.7), for example buki and buk2 from Clostridium acetobutylicum, also 10 accept acetate as a substrate (Hartmanis, M.G., J. Biol. Chem. 262:617-621 (1987)). Protein GenBank ID GI Number Organism ackA NP_416799.1 16130231 Escherichia coli Ack AAB18301.1 1491790 Clostridium acetobutylicum Ack AAA72042.1 349834 Methanosarcina thermophila purT AAC74919.1 1788155 Escherichia coli buki NP_349675 15896326 Clostridium acetobutylicum buk2 Q97111 20137415 Clostridium acetobutylicum The formation of acetyl-CoA from acetylphosphate is catalyzed by phosphotransacetylase (EC 2.3.1.8). The pta gene from E. coli encodes an enzyme that reversibly converts acetyl-CoA into acetyl-phosphate (Suzuki, T., Biochim. Biophys. Acta 191:559-569 (969)). 15 Additional acetyltransferase enzymes have been characterized in Bacillus subtilis (Rado and Hoch, Biochim. Biophys. Acta 321:114-125 (1973), Clostridium kluyveri (Stadtman, E., Methods Enzymol. 1:5896-599 (1955), and Thermotoga maritima (Bock et al., J. Bacteriol. 181:1861-1867 (1999)). This reaction is also catalyzed by some phosphotranbutyrylase enzymes (EC 2.3.1.19) including the ptb gene products from 20 Clostridium acetobutylicum (Wiesenbom et al., App. Environ. Microbiol. 55:317-322 176 (1989); Walter et al., Gene 134:107-111 (1993)). Additionalptb genes are found in butyrate-producing bacterium L2-50 (Louis et al., J. Bacteriol. 186:2099-2106 (2004) and Bacillus megaterium (Vazquez et al., Curr. Microbiol. 42:345-349 (2001). Protein GenBank ID GI Number Organism Pta NP_416800.1 71152910 Escherichia coli Pta P39646 730415 Bacillus subtilis Pta A5N801 146346896 Clostridium kluyveri Pta Q9XOL4 6685776 Thermotoga maritima Ptb NP_349676 34540484 Clostridium acetobutylicum Ptb AAR19757.1 butyrate-producing bacterium 38425288 L2-50 Ptb CAC07932.1 10046659 Bacillus megaterium 5 The acylation of acetate to acetyl-CoA is catalyzed by enzymes with acetyl-CoA synthetase activity. Two enzymes that catalyze this reaction are AMP-forming acetyl CoA synthetase (EC 6.2.1.1) and ADP-forming acetyl-CoA synthetase (EC 6.2.1.13). AMP-forming acetyl-CoA synthetase (ACS) is the predominant enzyme for activation of acetate to acetyl-CoA. Exemplary ACS enzymes are found in E. coli (Brown et al., J. 10 Gen. Microbiol. 102:327-336 (1977)), Ralstonia eutropha (Priefert and Steinbuchel, J. Bacteriol. 174:6590-6599 (1992)), Methanothermobacter thermautotrophicus (Ingram Smith and Smith, Archaea 2:95-107 (2007)), Salmonella enterica (Gulick et al., Biochemistry 42:2866-2873 (2003)) and Saccharomyces cerevisiae (Jogl and Tong, Biochemistry 43:1425-1431 (2004)). ADP-forming acetyl-CoA synthetases are reversible 15 enzymes with a generally broad substrate range (Musfeldt and Schonheit, J. Bacteriol. 184:636-644 (2002)). Two isozymes of ADP-forming acetyl-CoA synthetases are encoded in the Archaeoglobusfulgidus genome by are encoded by AF1211 and AF1983 (Musfeldt and Schonheit, supra (2002)). The enzyme from Haloarcula marismortui (annotated as a succinyl-CoA synthetase) also accepts acetate as a substrate and 20 reversibility of the enzyme was demonstrated (Brasen and Schonheit, Arch. Microbiol. 182:277-287 (2004)). The ACD encoded by PAE3250 from hyperthermophilic crenarchaeon Pyrobaculum aerophilum showed the broadest substrate range of all characterized ACDs, reacting with acetate, isobutyryl-CoA (preferred substrate) and phenylacetyl-CoA (Brasen and Schonheit, supra (2004)). Directed evolution or 25 engineering can be used to modify this enzyme to operate at the physiological temperature of the host organism. The enzymes from A. fulgidus, H. marismortui and P. aerophilum 177 have all been cloned, functionally expressed, and characterized in E. coli (Brasen and Schonheit, supra (2004); Musfeldt and Schonheit, supra (2002)). Additional candidates include the succinyl-CoA synthetase encoded by sucCD in E. coli (Buck et al., Biochemistry 24:6245-6252 (1985)) and the acyl-CoA ligase from Pseudomonasputida 5 (Fernandez-Valverde et al., Appl. Environ. Microbiol. 59:1149-1154 (1993)). The aforementioned proteins are tabulated below. Protein GenBank ID GI Number Organism acs AAC77039.1 1790505 Escherichia coli acoE AAA21945.1 141890 Ralstonia eutropha acsi ABC87079.1 86169671 Methanothermobacter thermautotrophicus acs! AAL23099.1 16422835 Salmonella enterica ACSI Q01574.2 257050994 Saccharomyces cerevisiae AF1211 NP_070039.1 11498810 Archaeoglobusfulgidus AF1983 NP_070807.1 11499565 Archaeoglobusfulgidus scs YP_135572.1 55377722 Haloarcula marismortui PAE3250 NP_560604.1 18313937 Pyrobaculum aerophilum str. IM2 sucC NP_415256.1 16128703 Escherichia coli sucD AAC73823.1 1786949 Escherichia coli paaF AAC24333.2 22711873 Pseudomonas putida The product yields per C-mol of substrate of microbial cells synthesizing reduced fermentation products such as 2,4-pentadienoate, butadiene, 1,3-butanediol or 3-buten- 1 10 ol, are limited by insufficient reducing equivalents in the carbohydrate feedstock. Reducing equivalents, or electrons, can be extracted from synthesis gas components such as CO and H 2 using carbon monoxide dehydrogenase (CODH) and hydrogenase enzymes, respectively. The reducing equivalents are then passed to acceptors such as oxidized ferredoxins, oxidized quinones, oxidized cytochromes, NAD(P)+, water, or hydrogen 15 peroxide to form reduced ferredoxin, reduced quinones, reduced cytochromes, NAD(P)H,

H

2 , or water, respectively. Reduced ferredoxin and NAD(P)H are particularly useful as they can serve as redox carriers for various Wood-Ljungdahl pathway and reductive TCA cycle enzymes.

178 Here, we show specific examples of how additional redox availability from CO and/or H 2 can improve the yields of reduced products such as 2,4-pentadienoate, butadiene, 1,3 butanediol or 3-buten-1-ol. In some embodiments of the invention, a combined feedstock strategy where syngas is 5 combined with a sugar-based feedstock or other carbon substrate can greatly improve the theoretical yields. In this co-feeding appoach, syngas components H 2 and CO can be utilized by the hydrogenase and CO dehydrogenase to generate reducing equivalents, that can be used to power chemical production pathways in which the carbons from sugar or other carbon substrates will be maximally conserved and the theoretical yields improved. 10 Such improvements provide environmental and economic benefits and greatly enhance sustainable chemical production. Herein below the enzymes and the corresponding genes used for extracting redox from synags components are described. CODH is a reversible enzyme that interconverts CO and CO 2 at the expense or gain of electrons. The natural physiological role of the CODH 15 in ACS/CODH complexes is to convert CO 2 to CO for incorporation into acetyl-CoA by acetyl-CoA synthase. Nevertheless, such CODH enzymes are suitable for the extraction of reducing equivalents from CO due to the reversible nature of such enzymes. Expressing such CODH enzymes in the absence of ACS allows them to operate in the direction opposite to their natural physiological role (i.e., CO oxidation). 20 In M. thermoacetica, C. hydrogenoformans, C. carboxidivorans P7, and several other organisms, additional CODH encoding genes are located outside of the ACS/CODH operons. These enzymes provide a means for extracting electrons (or reducing equivalents) from the conversion of carbon monoxide to carbon dioxide. The M thermoacetica gene (Genbank Accession Number: YP_430813) is expressed by itself in an 25 operon and is believed to transfer electrons from CO to an external mediator like ferredoxin in a "Ping-pong" reaction. The reduced mediator then couples to other reduced nicolinamide adenine dinucleotide phosphate (NAD(P)H) carriers or ferredoxin-dependent cellular processes (Ragsdale, Annals ofthe New York Academy ofSciences 1125: 129-136 (2008)). The genes encoding the C hydrogenoformans CODH-II and CooF, a 30 neighboring protein, were cloned and sequenced (Gonzalez and Robb, FEMS Microbiol Lett. 191:243-247 (2000)). The resulting complex was membrane-bound, although cytoplasmic fractions of CODH-II were shown to catalyze the formation of NADPH suggesting an anabolic role (Svetlitchnyi et al., J Bacteriol. 183:5134-5144 (2001)). The 179 crystal structure of the CODH-II is also available (Dobbek et al., Science 293:1281-1285 (2001)). Similar ACS-free CODH enzymes can be found in a diverse array of organisms including Geobacter metallireducens GS-1 5, Chlorobium phaeobacteroides DSM 266, Clostridium cellulolyticum H 10, Desulfovibrio desulfuricans subsp. desulfuricans str. 5 ATCC 27774, Pelobacter carbinolicus DSM 2380, and Campylobacter curvus 525.92. Protein GenBank ID GI Number Organism CODH (putative) YP_430813 83590804 Moorella thermoacetica CODH-II (CooS-II) YP_358957 78044574 Carboxydothermus hydrogenoformans CooF YP_358958 78045112 Carboxydothermus hydrogenoformans CODH (putative) ZP_05390164.1 255523193 Clostridium carboxidivorans P7 CcarbDRAFT_0341 ZP_05390341.1 255523371 Clostridium carboxidivorans P7 CcarbDRAFT_1756 ZP_05391756.1 255524806 Clostridium carboxidivorans P7 CcarbDRAFT_2944 ZP_05392944.1 255526020 Clostridium carboxidivorans P7 CODH YP_384856.1 78223109 Geobacter metallireducens GS-15 Cpha266_0148 YP_910642.1 119355998 Chlorobium (cytochrome c) phaeobacteroides DSM 266 Cpha266_0149 YP_910643.1 119355999 Chlorobium (CODH) phaeobacteroides DSM 266 Ccel_0438 YP_002504800.1 220927891 Clostridium cellulolyticum H10 Ddes_0382 YP_002478973.1 220903661 Desulfovibrio (CODH) desulfuricans subsp. desulfuricans str. A TCC 27774 Ddes_0381 (CooC) YP_002478972.1 220903660 Desulfovibrio desulfuricans subsp. desulfuricans str. A TCC 27774 Pcar_0057 YP_355490.1 7791767 Pelobacter carbinolicus (CODH) DSM 2380 Pcar_0058 YP_355491.1 7791766 Pelobacter carbinolicus 180 (CooC) DSM 2380 Pcar_0058 YP_355492.1 7791765 Pelobacter carbinolicus (HypA) DSM 2380 CooS (CODH) YP_001407343.1 154175407 Campylobacter curvus 525.92 In some cases, hydrogenase encoding genes are located adjacent to a CODH. In Rhodospirillum rubrum, the encoded CODH/hydrogenase proteins form a membrane bound enzyme complex that has been indicated to be a site where energy, in the form of a 5 proton gradient, is generated from the conversion of CO and H 2 0 to CO 2 and H 2 (Fox et al., JBacteriol. 178:6200-6208 (1996)). The CODH-I of C. hydrogenoformans and its adjacent genes have been proposed to catalyze a similar functional role based on their similarity to the R. rubrum CODH/hydrogenase gene cluster (Wu et al., PLoS Genet. 1:e65 (2005)). The C. hydrogenoformans CODH-I was also shown to exhibit intense CO 10 oxidation and CO 2 reduction activities when linked to an electrode (Parkin et al., J Am.Chem.Soc, 129:10328-10329 (2007)). The protein sequences of exemplary CODH and hydrogenase genes can be identified by the following GenBank accession numbers. Protein GenBank ID GI Number Organism CODH-I YP_360644 78043418 Carboxydothermus (CooS-I) hydrogenoformans CooF YP_360645 78044791 Carboxydothermus hydrogenoformans HypA YP_360646 78044340 Carboxydothermus hydrogenoformans CooH YP_360647 78043871 Carboxydothermus hydrogenoformans CooU YP_360648 78044023 Carboxydothermus hydrogenoformans CooX YP_360649 78043124 Carboxydothermus hydrogenoformans CooL YP_360650 78043938 Carboxydothermus hydrogenoformans CooK YP_360651 78044700 Carboxydothermus hydrogenoformans CooM YP_360652 78043942 Carboxydothermus hydrogenoformans CooC YP_360654.1 78043296 Carboxydothermus 181 Protein GenBank ID GI Number Organism hydrogenoformans CooA-1 YP_360655.1 78044021 Carboxvdothermus hydrogenoformans CooL AAC45118 1515468 Rhodospirillum rubrum CooX AAC45119 1515469 Rhodospirillum rubrum CooU AAC45120 1515470 Rhodospirillum rubrum CooH AAC45121 1498746 Rhodospirillum rubrum CooF AAC45122 1498747 Rhodospirillum rubrum CODH AAC45123 1498748 Rhodospirillum rubrum (CooS) CooC AAC45124 1498749 Rhodospirillum rubrum CooT AAC45125 1498750 Rhodospirillum rubrum CooJ AAC45126 1498751 Rhodospirillum rubrum Native to E. coli and other enteric bacteria are multiple genes encoding up to four hydrogenases (Sawers, G., Antonie Van Leeuwenhoek 66:57-88 (1994); Sawers et al., J Bacteriol. 164:1324-1331 (1985); Sawers and Boxer, Eur.JBiochem. 156:265-275 (1986); 5 Sawers et al., JBacteriol. 168:398-404 (1986)). Given the multiplicity of enzyme activities, E. coli or another host organism can provide sufficient hydrogenase activity to split incoming molecular hydrogen and reduce the corresponding acceptor. E. coli possesses two uptake hydrogenases, Hyd- 1 and Hyd-2, encoded by the hyaABCDEF and hybOABCDEFG gene clusters, respectively (Lukey et al., How E. coli is equipped to 10 oxidize hydrogen under different redox conditions, JBiol Chem published online Nov 16, 2009). Hyd-1 is oxygen-tolerant, irreversible, and is coupled to quinone reduction via the hyaC cytochrome. Hyd-2 is sensitive to 02, reversible, and transfers electrons to the periplasmic ferredoxin hybA which, in turn, reduces a quinone via the hybB integral membrane protein. Reduced quinones can serve as the source of electrons for fumarate 15 reductase in the reductive branch of the TCA cycle. Reduced ferredoxins can be used by enzymes such as NAD(P)H:ferredoxin oxidoreductases to generate NADPH or NADH. They can alternatively be used as the electron donor for reactions such as pyruvate ferredoxin oxidoreductase, AKG ferredoxin oxidoreductase, and 5,10-methylene-H4folate reductase. 20 182 Protein GenBank ID GI Number Organism HyaA AAC74057.1 1787206 Escherichia coli HyaB AAC74058.1 1787207 Escherichia coli HyaC AAC74059.1 1787208 Escherichia coli HyaD AAC74060.1 1787209 Escherichia coli HyaE AAC74061.1 1787210 Escherichia coli HyaF AAC74062.1 1787211 Escherichia coli Protein GenBank ID GI Number Organism HybO AAC76033.1 1789371 Escherichia coli HybA AAC76032.1 1789370 Escherichia coli HybB AAC76031.1 2367183 Escherichia coli HybC AAC76030.1 1789368 Escherichia coli HybD AAC76029.1 1789367 Escherichia coli HybE AAC76028.1 1789366 Escherichia coli HybF AAC76027.1 1789365 Escherichia coli HybG AAC76026.1 1789364 Escherichia coli The hydrogen-lyase systems of E. coli include hydrogenase 3, a membrane-bound enzyme 5 complex using ferredoxin as an acceptor, and hydrogenase 4 that also uses a ferredoxin acceptor. Hydrogenase. 3 and 4 are encoded by the hyc and hyf gene clusters, respectively. Hydrogenase 3 has been shown to be a reversible enzyme (Maeda et al., Appi Microbiol Biotechnol 76(5):1035-42 (2007)). Hydrogenase activity in E. coli is also dependent upon the expression of the hyp genes whose corresponding proteins are involved in the 10 assembly of the hydrogenase complexes (Jacobi et al., Arch.Microbiol 158:444-451 (1992); Rangarajan et al., J. Bacterial. 190:1447-1458 (2008)). Protein GenBank ID GI Number Organism HycA NP_417205 16130632 Escherichia coli HycB NP_417204 16130631 Escherichia coli HycC NP_417203 16130630 Escherichia coli HycD NP_417202 16130629 Escherichia coli HycE NP_417201 16130628 Escherichia coli HycF NP_417200 16130627 Escherichia coli 183 HycG NP_417199 16130626 Escherichia coli HycH NP_417198 16130625 Escherichia coli HycI NP_417197 16130624 Escherichia coli Protein GenBank ID GI Number Organism HyfA NP_416976 90111444 Escherichia coli HyfB NP_416977 16130407 Escherichia coli HyfC NP_416978 90111445 Escherichia coli HyfD NP_416979 16130409 Escherichia coli HyfE NP_416980 16130410 Escherichia coli HyfF NP_416981 16130411 Escherichia coli HyfG NP_416982 16130412 Escherichia coli HyfH NP_416983 16130413 Escherichia coli Hyfi NP_416984 16130414 Escherichia coli HyfJ NP_416985 90111446 Escherichia coli HyfR NP_416986 90111447 Escherichia coli Protein GenBank ID GI Number Organism HypA NP_417206 16130633 Escherichia coli HypB NP_417207 16130634 Escherichia coli HypC NP_417208 16130635 Escherichia coli HypD NP_417209 16130636 Escherichia coli HypE NP_417210 226524740 Escherichia coli HypF NP_417192 16130619 Escherichia coli The M. thermoacetica hydrogenases are suitable for a host that lacks sufficient 5 endogenous hydrogenase activity. M. thermoacetica can grow with CO 2 as the exclusive carbon source indicating that reducing equivalents are extracted from H 2 to enable acetyl CoA synthesis via the Wood-Ljungdahl pathway (Drake, H. L., J. Bacterial. 150:702-709 (1982); Drake and Daniel, Res. Microbiol. 155:869-883 (2004); Kellum and Drake, J. Bacterial. 160:466-469 (1984)) (see Figure 7). M. thermoacetica has homologs to several 10 hyp, hyc, and hyf genes from E. coli. The protein sequences encoded for by these genes are identified by the following GenBank accession numbers.

184 Proteins in M. thennoacetica whose genes are homologous to the E. coli hyp genes are shown below. Protein GenBank ID GI Number Organism Moth_2175 YP_431007 83590998 Moorella thermoacetica Moth_2176 YP_431008 83590999 Moorella thermoacetica Moth_2177 YP_431009 83591000 Moorella thermoacetica Moth_2178 YP_431010 83591001 Moorella thennoacetica Moth_2179 YP_431011 83591002 Moorella thermoacetica Moth_2180 YP_431012 83591003 Moorella thermoacetica Moth_2181 YP_431013 83591004 Moorella thermoacetica Proteins in M. thermoacetica that are homologous to the E. coli Hydrogenase 3 and/or 4 5 proteins are listed in the following table. Protein GenBank ID GI Number Organism Moth_2182 YP_431014 83591005 Moorella thermoacetica Moth_2183 YP_431015 83591006 Moorella thermoacetica Moth_2184 YP_431016 83591007 Moorella thermoacetica Moth_2185 YP_431017 83591008 Moorella thermoacetica Moth_2186 YP_431018 83591009 Moorella thermoacetica Moth_2187 YP_431019 83591010 Moorella thermoacetica Moth_2188 YP_431020 83591011 Moorella thermoacetica Moth_2189 YP_431021 83591012 Moorella thermoacetica Moth_2190 YP_431022 83591013 Moorella thermoacetica Moth_2191 YP_431023 83591014 Moorella thermoacetica Moth_2192 YP_431024 83591015 Moorella thermoacetica In addition, several gene clusters encoding hydrogenase functionality are present in M. thermoacetica and their corresponding protein sequences are provided below. Protein GenBank ID GI Number Organism Moth_0439 YP_429313 83589304 Moorella thermoacetica Moth_0440 YP_429314 83589305 Moorella thermoacetica Moth_0441 YP_429315 83589306 Moorella thennoacetica 185 Protein GenBank ID GI Number Organism Moth_0442 YP_429316 83589307 Moorella thermoacetica Moth_0809 YP_429670 83589661 Moorella thermoacetica Moth_0810 YP_429671 83589662 Moorella thennoacetica Moth_0811 YP_429672 83589663 Moorella thermoacetica Moth_0812 YP_429673 83589664 Moorella thermoacetica Moth_0814 YP_429674 83589665 Moorella thermoacetica Moth_0815 YP_429675 83589666 Moorella thermoacetica Moth_0816 YP_429676 83589667 Moorella thermoacetica Moth_1193 YP_430050 83590041 Moorella thermoacetica Moth_1194 YP_430051 83590042 Moorella thermoacetica Moth_1195 YP_430052 83590043 Moorella thermoacetica Moth_ 1196 YP_430053 83590044 Moorella thennoacetica Moth_1717 YP_430562 83590553 Moorella thennoacetica Moth_1718 YP_430563 83590554 Moorella thermoacetica Moth 1719 YP_430564 83590555 Moorella thennoacetica Moth_1883 YP_430726 83590717 Moorella thennoacetica Moth_1884 YP_430727 83590718 Moorella thermoacetica Moth_1885 YP_430728 83590719 Moorella thermoacetica Moth_1886 YP_430729 83590720 Moorella thennoacetica Moth_1887 YP_430730 83590721 Moorella thennoacetica Moth_1888 YP_430731 83590722 Moorella thermoacetica Moth_1452 YP_430305 83590296 Moorella thermoacetica Moth_1453 YP_430306 83590297 Moorella thermoacetica Moth_1454 YP_430307 83590298 Moorella thennoacetica Ralstonia eutropha H16 uses hydrogen as an energy source with oxygen as a terminal electron acceptor. Its membrane-bound uptake [NiFe]-hydrogenase is an "02-tolerant" hydrogenase (Cracknell, et al. Proc Nat Acad Sci, 106(49) 20681-20686 (2009)) that is 5 periplasmically-oriented and connected to the respiratory chain via a b-type cytochrome (Schink and Schlegel, Biochin. Biophys. Acta, 567, 315-324 (1979); Bernhard et al., Eur. J. Biochem. 248, 179-186 (1997)). R. eutropha also contains an 0 2 -tolerant soluble hydrogenase encoded by the Hox operon which is cytoplasmic and directly reduces NAD+ at the expense of hydrogen (Schneider and Schlegel, Biochim. Biophys. Acta 452, 66-80 186 (1976); Burgdorf, J. Bact. 187(9) 3122-3132(2005)). Soluble hydrogenase enzymes are additionally present in several other organisms including Geobacter sulfitrreducens (Coppi, Microbiology 151, 1239-1254 (2005)), Synechocystis str. PCC 6803 (Germer, J. Biol. Chem., 284(52), 36462-36472 (2009)), and Thiocapsa roseopersicina (Rakhely, 5 Appl. Environ. Microbiol. 70(2) 722-728 (2004)). The Synechocystis enzyme is capable of generating NADPH from hydrogen. Overexpression of both the Hox operon from Synechocystis str. PCC 6803 and the accessory genes encoded by the Hyp operon from Nostoc sp. PCC 7120 led to increased hydrogenase activity compared to expression of the Hox genes alone (Germer, J. Biol. Chem. 284(52), 36462-36472 (2009)). Protein GenBank ID GI Number Organism HoxF NP_942727.1 38637753 Ralstonia eutropha H16 HoxU NP_942728.1 38637754 Ralstonia eutropha H16 HoxY NP_942729.1 38637755 Ralstonia eutropha H16 HoxH NP_942730.1 38637756 Ralstonia eutropha H16 HoxW NP_942731.1 38637757 Ralstonia eutropha H16 Hox] NP_942732.1 38637758 Ralstonia eutropha H16 HoxE NP_953767.1 39997816 Geobacter sulfurreducens HoxF NP_953766.1 39997815 Geobacter sulfurreducens HoxU NP_953765.1 39997814 Geobacter sulfurreducens HoxY NP_953764.1 39997813 Geobacter sulfurreducens HoxH NP_953763.1 39997812 Geobacter sulfurreducens GSU2717 NP_953762.1 39997811 Geobacter sulfurreducens HoxE NP_441418.1 16330690 Synechocystis str. PCC 6803 HoxF NP_441417.1 16330689 Synechocystis str. PCC 6803 Unknown NP_441416.1 16330688 Synechocystis str. PCC 6803 function HoxU NP_441415.1 16330687 Synechocystis str. PCC 6803 HoxY NP_441414.1 16330686 Synechocystis str. PCC 6803 Unknown NP_441413.1 16330685 Synechocystis str. PCC 6803 function Unknown NP_441412.1 16330684 Synechocystis str. PCC 6803 function HoxH NP_441411.1 16330683 Synechocystis str. PCC 6803 HypF NP_484737.1 17228189 Nostoc sp. PCC 7120 187 HypC NP_484738.1 17228190 Nostoc sp. PCC 7120 HypD NP_484739.1 17228191 Nostoc sp. PCC 7120 Unknown NP_484740.1 17228192 Nostoc sp. PCC 7120 function HypE NP_484741.1 17228193 Nostoc sp. PCC 7120 HypA NP_484742.1 17228194 Nostoc sp. PCC 7120 HypB NP_484743.1 17228195 Nostoc sp. PCC 7120 HoxlE AAP50519.1 37787351 Thiocapsa roseopersicina HoxIF AAP50520.1 37787352 Thiocapsa roseopersicina Hox1U AAP50521.1 37787353 Thiocapsa roseopersicina Hox1Y AAP50522.1 37787354 Thiocapsa roseopersicina HoxlH AAP50523.1 37787355 Thiocapsa roseopersicina Several enzymes and the corresponding genes used for fixing carbon dioxide to either pyruvate or phosphoenolpyruvate to form the TCA cycle intermediates, oxaloacetate or malate are described below. 5 Carboxylation of phosphoenolpyruvate to oxaloacetate is catalyzed by phosphoenolpyruvate carboxylase. Exemplary PEP carboxylase enzymes are encoded by ppc in E. coli (Kai et al., Arch. Biochem. Biophys. 414:170-179 (2003), ppcA in Methylobacterium extorquens AM] (Arps et al., J. Bacteriol. 175:3776-3783 (1993), and ppc in Corynebacterium glutamicum (Eikmanns et al., Mol. Gen. Genet. 218:330-339 10 (1989). Protein GenBank ID GI Number Organism Ppc NP_418391 16131794 Escherichia coli ppcA AAB58883 28572162 Methylobacterium extorquens Ppc ABB53270 80973080 Corynebacterium glutamicumn An alternative enzyme for converting phosphoenolpyruvate to oxaloacetate is PEP carboxykinase, which simultaneously forms an ATP while carboxylating PEP. In most organisms PEP carboxykinase serves a gluconeogenic function and converts oxaloacetate 15 to PEP at the expense of one ATP. S. cerevisiae is one such organism whose native PEP carboxykinase, PCK1, serves a gluconeogenic role (Valdes-Hevia et al., FEBS Lett. 258:313-316 (1989). E. coli is another such organism, as the role of PEP carboxykinase in 188 producing oxaloacetate is believed to be minor when compared to PEP carboxylase, which does not form ATP, possibly due to the higher Km for bicarbonate of PEP carboxykinase (Kim et al., AppL. Environ. Microbiol. 70:1238-1241 (2004)). Nevertheless, activity of the native E. coli PEP carboxykinase from PEP towards oxaloacetate has been recently 5 demonstrated inppc mutants of E. coli K-12 (Kwon et al., J. Microbiol. Biotechnol. 16:1448-1452 (2006)). These strains exhibited no growth defects and had increased succinate production at high NaHCO 3 concentrations. Mutant strains of E. coli can adopt Pck as the dominant C02-fixing enzyme following adaptive evolution (Zhang et al. 2009). In some organisms, particularly rumen bacteria, PEP carboxykinase is quite 10 efficient in producing oxaloacetate from PEP and generating ATP. Examples of PEP carboxykinase genes that have been cloned into E. coli include those from Mannheimia succiniciproducens (Lee et al., Biotechnol. Bioprocess Eng. 7:95-99 (2002)), Anaerobiospirillum succiniciproducens (Laivenieks et al., Appl. Environ. Microbio. 63:2273-2280 (1997), and Actinobacillus succinogenes (Kim et al. supra). The PEP 15 carboxykinase enzyme encoded by Haemophilus influenza is effective at forming oxaloacetate from PEP. Protein GenBank ID GI Number Organism PCK1 NP_013023 6322950 Saccharomyces cerevisiae pck NP_417862.1 16131280 Escherichia coli pckA YP_089485.1 52426348 Mannheimia succiniciproducens pckA 009460.1 3122621 Anaerobiospirillum succiniciproducens pckA Q6W6X5 75440571 Actinobacillus succinogenes pckA P43923.1 1172573 Haemophilus influenza Pyruvate carboxylase (EC 6.4.1.1) directly converts pyruvate to oxaloacetate at the cost of one ATP. Pyruvate carboxylase enzymes are encoded by PYC1 (Walker et al., Biochem. Biophys. Res. Commun. 176:1210-1217 (1991) and PYC2 (Walker et al., supra) in 20 Saccharomyces cerevisiae, and pyc in Mycobacterium smegmatis (Mukhopadhyay and Purwantini, Biochim. Biophys. Acta 1475:191-206 (2000)). Protein GenBank ID GI Number Organism PYC1 NP_011453 6321376 Saccharomyces cerevisiae PYC2 NP_009777 6319695 Saccharomyces cerevisiae 189 Protein GenBank ID GI Number Organism Pyc YP_890857.1 118470447 Mycobacterium smegmatis Malic enzyme can be applied to convert CO 2 and pyruvate to malate at the expense of one reducing equivalent. Malic enzymes for this purpose can include, without limitation, malic enzyme (NAD-dependent) and malic enzyme (NADP-dependent). For example, one of 5 the E. coli malic enzymes (Takeo, J. Biochem. 66:379-387 (1969)) or a similar enzyme with higher activity can be expressed to enable the conversion of pyruvate and CO 2 to malate. By fixing carbon to pyruvate as opposed to PEP, malic enzyme allows the high energy phosphate bond from PEP to be conserved by pyruvate kinase whereby ATP is generated in the formation of pyruvate or by the phosphotransferase system for glucose 10 transport. Although malic enzyme is typically assumed to operate in the direction of pyruvate formation from malate, overexpression of the NAD-dependent enzyme, encoded by maeA, has been demonstrated to increase succinate production in E. coli while restoring the lethal pfl- ldhA phenotype under anaerobic conditions by operating in the carbon-fixing direction (Stols and Donnelly, AppL. Environ. Microbiol. 63(7) 2695-2701 15 (1997)). A similar observation was made upon overexpressing the malic enzyme from Ascaris suum in E. coli (Stols et al., Appl. Biochem. Biotechnol. 63-65(1), 153-158 (1997)). The second E. coli malic enzyme, encoded by maeB, is NADP-dependent and also decarboxylates oxaloacetate and other alpha-keto acids (Iwakura et al., J. Biochem. 85(5):1355-65 (1979)). 20 Protein GenBank ID GI Number Organism maeA NP_415996 90111281 Escherichia coli maeB NP_416958 16130388 Escherichia coli NAD-ME P27443 126732 Ascaris suum The enzymes used for converting oxaloacetate (formed from, for example, PEP carboxylase, PEP carboxykinase, or pyruvate carboxylase) or malate (formed from, for example, malic enzyme or malate dehydrogenase) to succinyl-CoA via the reductive 25 branch of the TCA cycle are malate dehydrogenase, fumarate dehydratase (fumarase), fumarate reductase, and succinyl-CoA transferase. The genes for each of the enzymes are described herein above.

190 Enzymes, genes and methods for engineering pathways from succinyl-CoA to various products into a microorganism are now known in the art. The additional reducing equivalents obtained from CO and/or H 2 , as disclosed herein, improve the yields of 2,4 pentadienoate, butadiene, 1,3-butanediol or 3-buten- I -ol when utilizing carbohydrate 5 based feedstock. Enzymes, genes and methods for engineering pathways from glycolysis intermediates to various products into a microorganism are known in the art. The additional reducing equivalents obtained from CO and H 2 , as described herein, improve the yields of all these products on carbohydrates. 10 EXAMPLE X Methods for Handling CO and Anaerobic Cultures This example describes methods used in handling CO and anaerobic cultures. A. Handling of CO in small quantities for assays and small cultures. CO is an 15 odorless, colorless and tasteless gas that is a poison. Therefore, cultures and assays that utilized CO required special handling. Several assays, including CO oxidation, acetyl CoA synthesis, CO concentration using myoglobin, and CO tolerance/utilization in small batch cultures, called for small quantities of the CO gas that were dispensed and handled within a fume hood. Biochemical assays called for saturating very small quantities (<2 20 mL) of the biochemical assay medium or buffer with CO and then performing the assay. All of the CO handling steps were performed in a fume hood with the sash set at the proper height and blower turned on; CO was dispensed from a compressed gas cylinder and the regulator connected to a Schlenk line. The latter ensures that equal concentrations of CO were dispensed to each of several possible cuvettes or vials. The Schlenk line was 25 set up containing an oxygen scrubber on the input side and an oil pressure release bubbler and vent on the other side. Assay cuvettes were both anaerobic and CO-containing. Threfore, the assay cuvettes were tightly sealed with a rubber stopper and reagents were added or removed using gas-tight needles and syringes. Secondly, small (-50 mL) cultures were grown with saturating CO in tightly stoppered serum bottles. As with the 30 biochemical assays, the CO-saturated microbial cultures were equilibrated in the fume hood using the Schlenk line setup. Both the biochemical assays and microbial cultures were in portable, sealed containers and in small volumes making for safe handling outside of the fume hood. The compressed CO tank was adjacent to the fume hood.

191 Typically, a Schlenk line was used to dispense CO to cuvettes, each vented. Rubber stoppers on the cuvettes were pierced with 19 or 20 gage disposable syringe needles and were vented with the same. An oil bubbler was used with a CO tank and oxygen scrubber. The glass or quartz spectrophotometer cuvettes have a circular hole on top into which a 5 Kontes stopper sleeve, Sz7 774250-0007 was fitted. The CO detector unit was positioned proximal to the fume hood. B. Handling of CO in larger quantities fed to large-scale cultures. Fermentation cultures are fed either CO or a mixture of CO and H 2 to simulate syngas as a feedstock in fermentative production. Therefore, quantities of cells ranging from 1 liter to several liters 10 can include the addition of CO gas to increase the dissolved concentration of CO in the medium. In these circumstances, fairly large and continuously administered quantities of CO gas are added to the cultures. At different points, the cultures are harvested or samples removed. Alternatively, cells are harvested with an integrated continuous flow centrifuge that is part of the fermenter. 15 The fermentative processes are carried out under anaerobic conditions. In some cases, it is uneconomical to pump oxygen or air into fermenters to ensure adequate oxygen saturation to provide a respiratory environment. In addition, the reducing power generated during anaerobic fermentation may be needed in product formation rather than respiration. Furthermore, many of the enzymes for various pathways are oxygen-sensitive to varying 20 degrees, Classic acetogens such as M. thermoacetica are obligate anaerobes and the enzymes in the Wood-Ljungdahl pathway are highly sensitive to irreversible inactivation by molecular oxygen. While there are oxygen-tolerant acetogens, the repertoire of enzymes in the Wood-Ljungdahl pathway might be incompatible in the presence of oxygen because most are metallo-enzymes, key components are ferredoxins, and 25 regulation can divert metabolism away from the Wood-Ljungdahl pathway to maximize energy acquisition. At the same time, cells in culture act as oxygen scavengers that moderate the need for extreme measures in the presence of large cell growth. C. Anaerobic chamber and conditions. Exemplary anaerobic chambers are available commercially (see, for example, Vacuum Atmospheres Company, Hawthorne CA; 30 MBraun, Newburyport MA). Conditions included an 02 concentration of 1 ppm or less and 1 atm pure N 2 . In one example, 3 oxygen scrubbers/catalyst regenerators were used, and the chamber included an 02 electrode (such as Teledyne; City of Industry CA). Nearly all items and reagents were cycled four times in the airlock of the chamber prior to 192 opening the inner chamber door. Reagents with a volume >5mL were sparged with pure N2 prior to introduction into the chamber. Gloves are changed twice/yr and the catalyst containers were regenerated periodically when the chamber displays increasingly sluggish response to changes in oxygen levels. The chamber's pressure was controlled through 5 one-way valves activated by solenoids. This feature allowed setting the chamber pressure at a level higher than the surroundings to allow transfer of very small tubes through the purge valve. The anaerobic chambers achieved levels of 02 that were consistently very low and were needed for highly oxygen sensitive anaerobic conditions. However, growth and handling 10 of cells does not usually require such precautions. In an alternative anaerobic chamber configuration, platinum or palladium can be used as a catalyst that requires some hydrogen gas in the mix. Instead of using solenoid valves, pressure release can be controlled by a bubbler. Instead of using instrument-based 02 monitoring, test strips can be used instead. D. Anaerobic microbiology. Small cultures were handled as described above for CO 15 handling. In particular, serum or media bottles are fitted with thick rubber stoppers and aluminum crimps are employed to seal the bottle. Medium, such as Terrific Broth, is made in a conventional manner and dispensed to an appropriately sized serum bottle. The bottles are sparged with nitrogen for -30 min of moderate bubbling. This removes most of the oxygen from the medium and, after this step, each bottle is capped with a rubber 20 stopper (such as Bellco 20 mm septum stoppers; Bellco, Vineland, NJ) and crimp-sealed (Bellco 20 mm). Then the bottles of medium are autoclaved using a slow (liquid) exhaust cycle. At least sometimes a needle can be poked through the stopper to provide exhaust during autoclaving; the needle needs to be removed immediately upon removal from the autoclave. The sterile medium has the remaining medium components, for example buffer 25 or antibiotics, added via syringe and needle. Prior to addition of reducing agents, the bottles are equilibrated for 30 - 60 minutes with nitrogen (or CO depending upon use). A reducing agent such as a 100 x 150 mM sodium sulfide, 200 mM cysteine-HCI is added. This is made by weighing the sodium sulfide into a dry beaker and the cysteine into a serum bottle, bringing both into the anaerobic chamber, dissolving the sodium sulfide into 30 anaerobic water, then adding this to the cysteine in the serum bottle. The bottle is stoppered immediately as the sodium sulfide solution generates hydrogen sulfide gas upon contact with the cysteine. When injecting into the culture, a syringe filter is used to sterilize the solution. Other components are added through syringe needles, such as B 12 193 (10 iM cyanocobalamin), nickel chloride (NiCl 2 , 20 microM final concentration from a 40 mM stock made in anaerobic water in the chamber and sterilized by autoclaving or by using a syringe filter upon injection into the culture), and ferrous ammonium sulfate (final concentration needed is 100 iM-made as 100- 1000x stock solution in anaerobic water in 5 the chamber and sterilized by autoclaving or by using a syringe filter upon injection into the culture). To facilitate faster growth under anaerobic conditions, the 1 liter bottles were inoculated with 50 mL of a preculture grown anaerobically. Induction of the pAl-lacOl promoter in the vectors was performed by addition of isopropyl p-D-1 thiogalactopyranoside (IPTG) to a final concentration of 0.2 mM and was carried out for 10 about 3 hrs. Large cultures can be grown in larger bottles using continuous gas addition while bubbling. A rubber stopper with a metal bubbler is placed in the bottle after medium addition and sparged with nitrogen for 30 minutes or more prior to setting up the rest of the bottle. Each bottle is put together such that a sterile filter will sterilize the gas bubbled 15 in and the hoses on the bottles are compressible with small C clamps. Medium and cells are stirred with magnetic stir bars. Once all medium components and cells are added, the bottles are incubated in an incubator in room air but with continuous nitrogen sparging into the bottles. EXAMPLE XI 20 CO oxidation (CODH) Assay This example describes assay methods for measuring CO oxidation (CO dehydrogenase; CODH). The 7 gene CODH/ACS operon of Moorella thermoacetica was cloned into E. coli expression vectors. The intact ~10 kbp DNA fragment was cloned, and it is likely that 25 some of the genes in this region are expressed from their own endogenous promoters and all contain endogenous ribosomal binding sites. These clones were assayed for CO oxidation, using an assay that quantitatively measures CODH activity. Antisera to the M. thermoacetica gene products was used for Western blots to estimate specific activity. M. thermoacetica is Gram positive, and ribosome binding site elements are expected to work 30 well in E. coli. This activity, described below in more detail, was estimated to be ~1/50th of the M. thermoacetica specific activity. It is possible that CODH activity of recombinant E. coli cells could be limited by the fact that M. thermoacetica enzymes have temperature optima around 55oC. Therefore, a mesophilic CODH/ACS pathway could be 194 advantageous such as the close relative of Moorella that is mesophilic and does have an apparently intact CODH/ACS operon and a Wood-Ljungdahl pathway, Desulfitobacterium hafniense. Acetogens as potential host organisms include, but are not limited to, Rhodospirillum rubrum, Moorella thermoacetica and Desulfitobacterium hafnliense. 5 CO oxidation is both the most sensitive and most robust of the CODH/ACS assays. It is likely that an E. coli-based syngas using system will ultimately need to be about as anaerobic as Clostridial (i.e., Moorella) systems, especially for maximal activity. Improvement in CODH should be possible but will ultimately be limited by the solubility of CO gas in water. 10 Initially, each of the genes was cloned individually into expression vectors. Combined expression units for multiple subunits/1 complex were generated. Expression in E. coli at the protein level was determined. Both combined M. thermoacetica CODH/ACS operons and individual expression clones were made. CO oxidation assay. This assay is one of the simpler, reliable, and more versatile assays 15 of enzymatic activities within the Wood-Ljungdahl pathway and tests CODH (Seravalli et al., Biochemistry 43:3944-3955 (2004)). A typical activity of M. thermoacetica CODH specific activity is 500 U at 55oC or ~60U at 25oC. This assay employs reduction of methyl viologen in the presence of CO. This is measured at 578 nm in stoppered, anaerobic, glass cuvettes. 20 In more detail, glass rubber stoppered cuvettes were prepared after first washing the cuvette four times in deionized water and one time with acetone. A small amount of vacuum grease was smeared on the top of the rubber gasket. The cuvette was gassed with CO, dried 10 min with a 22 Ga. needle plus an exhaust needle. A volume of 0.98 miL of reaction buffer (50 mM Hepes, pH 8.5, 2mM dithiothreitol (DTT) was added using a 22 25 Ga. needle, with exhaust needled, and 100%CO. Methyl viologen (CH 3 viologen) stock was 1 M in water. Each assay used 20 microliters for 20 mM final concentration. When methyl viologen was added, an 18 Ga needle (partial) was used as a jacket to facilitate use of a Hamilton syringe to withdraw the CH 3 viologen. 4 -5 aliquots were drawn up and discarded to wash and gas equilibrate the syringe. A small amount of sodium dithionite 30 (0.1 M stock) was added when making up the CH 3 viologen stock to slightly reduce the

CH

3 viologen. The temperature was equilibrated to 55'C in a heated Otis spectrophotometer (Bogart GA). A blank reaction (CH 3 viologen + buffer) was run first to 195 measure the base rate of CH 3 viologen reduction. Crude E. coli cell extracts of ACS90 and ACS91 (CODH-ACS operon of M. thennoacetica with and without, respectively, the first cooC). 10 microliters of extract were added at a time, mixed and assayed. Reduced

CH

3 viologen turns purple. The results of an assay are shown in Table 1. 5 Table I. Crude extract CO Oxidation Activities. ACS90 7.7 mg/mi ACS91 11.8 mg/mI Mta98 9.8 mg/r I Mta99 11.2 mg/rI Extract VI QDL nI m ACS90 10 microliters 0.073 0.376 0.049 ACS91 l 10microliters 0.096 0.494 0.042 Mta99 10 microliters 0.0031 0.016 0.0014 A CS90 10microliters 0.099 0.51 0.066 Mta99 25 microliters 0.012 0.025 0.0022 CS91 25 microliters 0.215 0.443 0.037 Mta98 25 microliters 0.019 0.039 0.004 ACS91 10 microliters 0.129 0.66 0.056 Ave rages ACS90 0.057 U/mg ACS91 0.04S U/mg Mta99 0.0018 U/mg Mta98/Mta99 are E. coli MG 1655 strains that express methanol methyltransferase genes 10 from M. thermoacetia and, therefore, are negative controls for the ACS90 ACS91 E. coli strains that contain M. thermoacetica CODH operons. If 1% of the cellular protein is CODH, then these figures would be approximately 100X less than the 500 U/mg activity of pure M. thermoacetica CODH. Actual estimates based on Western blots are 0.5% of the cellular protein, so the activity is about 50X less than for 15 M. thermoacetica CODH. Nevertheless, this experiment demonstrates CO oxidation activity in recombinant E. coli with a much smaller amount in the negative controls. The small amount of CO oxidation (CH3 viologen reduction) seen in the negative controls indicates that E. coli may have a limited ability to reduce CH3 viologen. To estimate the final concentrations of CODH and Mtr proteins, SDS-PAGE followed by 20 Western blot analyses were performed on the same cell extracts used in the CO oxidation, ACS, methyltransferase, and corrinoid Fe-S assays. The antisera used were polyclonal to purified M. thermoacetica CODH-ACS and Mtr proteins and were visualized using an 196 alkaline phosphatase-linked goat-anti-rabbit secondary antibody. The Westerns were performed and results are shown in Figure 9. The amounts of CODH in ACS90 and ACS91 were estimated at 50 ng by comparison to the control lanes. Expression of CODH-ACS operon genes including 2 CODH subunits and the methyltransferase were 5 confirmed via Western blot analysis. Therefore, the recombinant E. coli cells express multiple components of a 7 gene operon. In addition, both the methyltransferase and corrinoid iron sulfur protein were active in the same recombinant E. coli cells. These proteins are part of the same operon cloned into the same cells. The CO oxidation assays were repeated using extracts of Moorella thermoacetica cells for 10 the positive controls. Though CODH activity in E. coli ACS90 and ACS91 was measurable, it was at about 130 - 150 X lower than the M. thermoacetica control. The results of the assay are shown in Figure 10. Briefly, cells (M. thermoacetica or E. coli with the CODH/ACS operon; ACS90 or ACS91 or empty vector: pZA33S) were grown and extracts prepared as described above. Assays were performed as described above at 15 55oC at various times on the day the extracts were prepared. Reduction of methylviologen was followed at 578 nm over a 120 sec time course. These results describe the CO oxidation (CODH) assay and results. Recombinant E. coli cells expressed CO oxidation activity as measured by the methyl viologen reduction assay. EXAMPLE XII 20 E. coli CO Tolerance Experiment and CO Concentration Assay (myoglobin assay) This example describes the tolerance of E. coli for high concentrations of CO. To test whether or not E. coli can grow anaerobically in the presence of saturating amounts of CO, cultures were set up in 120 ml serum bottles with 50 ml of Terrific Broth 25 medium (plus reducing solution, NiCl2, Fe(II)NH4SO4, cyanocobalamin, IPTG, and chloramphenicol) as described above for anaerobic microbiology in small volumes. One half of these bottles were equilibrated with nitrogen gas for 30 min. and one half was equilibrated with CO gas for 30 min. An empty vector (pZA33) was used as a control, and cultures containing the pZA33 empty vector as well as both ACS90 and ACS91 were 30 tested with both N 2 and CO. All were inoculated and grown for 36 hrs with shaking (250 rpm) at 37'C. At the end of the 36 hour period, examination of the flasks showed high amounts of growth in all. The bulk of the observed growth occurred overnight with a long lag.

197 Given that all cultures appeared to grow well in the presence of CO, the final CO concentrations were confirmed. This was performed using an assay of the spectral shift of myoglobin upon exposure to CO. Myoglobin reduced with sodium dithionite has an absorbance peak at 435 nm; this peak is shifted to 423 nm with CO. Due to the low 5 wavelength and need to record a whole spectrum from 300 nm on upwards, quartz cuvettes must be used. CO concentration is measured against a standard curve and depends upon the Henry's Law constant for CO of maximum water solubility = 970 micromolar at 20oC and 1 atm. For the myoglobin test of CO concentration, cuvettes were washed 1OX with water, IX 10 with acetone, and then stoppered as with the CODH assay. N2 was blown into the cuvettes for -10 min. A volume of 1 ml of anaerobic buffer (HEPES, pH 8.0, 2mM DTT) was added to the blank (not equilibrated with CO) with a Hamilton syringe. A volume of 10 microliter myoglobin (-1 mM-can be varied, just need a fairly large amount) and 1 microliter dithionite (20 mM stock) were added. A CO standard curve was made using 15 CO saturated buffer added at 1 microliter increments. Peak height and shift was recorded for each increment. The cultures tested were pZA33/CO, ACS90/CO, and ACS91/CO. Each of these was added in I microliter increments to the same cuvette. Midway through the experiment a second cuvette was set up and used, The results are shown in Table I. Table I. Carbon Monoxide Concentrations, 36 hrs. Strain and Growth Conditions Final CO concentration (micromolar) pZA33-CO 930 ACS9O-CO638 494 734 883 ave 687 SD 164 ACS91-CO 728 812 760 611 ave. 728 2 0 .. .S.. .D ....1. .... .. .- -. . ..... .. ... .. ...: .. .... ... .. ... .... ..8. ... ... ... .. . .... ... ..

198 The results shown in Table II indicate that the cultures grew whether or not a strain was cultured in the presence of CO or not. These results indicate that E. coli can tolerate exposure to CO under anaerobic conditions and that E. coli cells expressing the CODH ACS operon can metabolize some of the CO. 5 These results demonstrate that E. coli cells, whether expressing CODH/ACS or not, were able to grow in the presence of saturating amounts of CO. Furthermore, these grew equally well as the controls in nitrogen in place of CO. This experiment demonstrated that laboratory strains of E. coli are insensitive to CO at the levels achievable in a syngas project performed at normal atmospheric pressure. In addition, preliminary experiments 10 indicated that the recombinant E. coli cells expressing CODH/ACS actually consumed some CO, probably by oxidation to carbon dioxide. EXAMPLE XI1 Pathways to 1,3-butanediol, propylene and crotyl alcohol Pathways to 1,3-butanediol, propylene and crotyl alcohol are shown in Figure 7. These 15 pathways can begin with the initiation of fatty acid biosynthesis, in which malonyl-ACP is condensed with acetyl-CoA or acetyl-ACP to form acetoacetyl-ACP (step A). The second step involves reduction of acetoacetyl-ACP to 3-hydroxybutyryl-ACP. Following dehydration to crotonyl-ACP and another reduction, butyryl-ACP is formed. The chain elongation typically continues with further addition of malonyl-ACP until a long-chain 20 acyl chain is formed, which is then hydrolyzed by a thioesterase into a free C 16 fatty acid. Bacterial fatty acid synthesis systems (FAS II) utilize discreet proteins for each step, whereas fungal and mammalian fatty acid synthesis systems (FAS I) utilize complex multifunctional proteins. The pathways utilize one or more enzymes of fatty acid biosynthesis to produce the C3 and C4 products, propylene, 1,3-butanediol and crotyl 25 alcohol. Several pathways are shown in Figure 7 for converting acetoacetyl-ACP to 1,3-butanediol. In some pathways, acetoacetyl-ACP is first converted to acetoacetyl-CoA (step D). Alternatively, acetoacetyl-CoA can also be synthesized from acetyl-CoA and malonyl CoA by acetoacetyl-CoA synthase (EC 2.3.1.194). Acetoacetyl-CoA can then be 30 hydrolyzed to acetoacetate by a CoA transferase, hydrolase or synthetase (step E). Acetoacetate is then reduced to 3-oxobutyraldehyde by a carboxylic acid reductase (step F). Alternately, acetoacetyl-CoA is converted directly to 3-oxobutyraldehyde by a CoA- 199 dependent aldehyde dehydrogenase (step I). In yet another embodiement, acetoacetyl-ACP is converted directly to 3-oxobutyraldehyde by an acyl-ACP reductase (step J), 3 Oxobutyraldehyde is further reduced to 1,3-butanediol via a 4-hydroxy-2-butanone or 3 hydroxybutyraldehyde intermediate (steps G and S, or steps R and AA). Another option is 5 the direct conversion of acetoacetyl-CoA to 4-hydroxy-2-butanone by a bifunctional enzyme with aldehyde dehydrogenase/alcohol dehydrogenase activity (step K). Pathways to 1,3-butanediol can also proceed through a 3-hydroxybutyryl-CoA intermediate. This intermediate is formed by the reduction of acetoacetyl-CoA (step P) or the transacylation of 3-hydroxybutyryl-ACP (step X). 3-Hydroxybutyryl-CoA is further converted to 3 10 hydroxybutyrate (step Y), 3-hydroxybutyraldehyde (step N) or 1,3-butanediol (step 0). Alternately, the 3-hydroxybutyrate intermediate is formed from acetoacetate (step Q) or via hydrolysis of 3-hydroxybutyryl-ACP (step L). The 3-hydroxybutyraldehyde intermediate is also the product of 3-hydroxybutyrl-ACP reductase (step M). Figure 7 also shows pathways from malonyl-ACP to crotyl alcohol. In one embodiment, 15 fatty acid initiation and extension enzymes produce the crotonyl-ACP intermediate (steps A, B, C). Crotonyl-ACP is then transacylated, hydrolyzed or reduced to crotonyl-CoA, crotonate or crotonaldehyde, respectively (steps AE, T, U). Crotonyl-CoA and crotonate are interconverted by a CoA hydrolase, transferase or synthetase (step AF). Crotonate is reduced to crotonaldehyde by a carboxylic acid reductase (step AG). In the final step of all 20 pathways, crotonaldehyde is reduced to crotyl alcohol by an aldehyde reductase in step AH. Numerous alternate pathways enumerated in the table below are also encompassed in the invention. Crotonyl-CoA can be reduced to crotonaldehyde or crotyl alcohol (steps V, W). Alternately, the 3-hydroxybutyryl intermediates of the previously described 1,3 butanediol pathways can also be converted to crotyl alcohol precursors. For example, 25 dehydration of 3-hydroxybutyryl-CoA, 3-hydroxybutyrate or 3-hydroxybutyraldehyde yields crotonyl-CoA, crotonate or crotonaldehyde, respectively (step AB, AC, AD). Pathways to propylene are also shown in Figure 7. In one embodiment, the crotonaldehyde intermediate is decarbonylated to propylene (step AO). In another embodiment, the 3 hydroxybutyrate intermediate is converted to propylene by an alkene-forming 30 decarboxylase (step AR). Decarboxylation of crotonate also forms propylene (step AQ). In yet another embodiment, the enzymes of fatty acid biosynthesis further convert crotonyl ACP to butyryl-ACP (step AL), which can then be transacylated to butyryl-CoA (step AI) or hydrolyzed to butyrate (step AP). The butyryl-CoA intermediate is also formed from 200 the reduction of crotonyl-CoA (step AM). The butyrate intermediate is also formed from reduction of crotonate or removal of the CoA moiety of butyryl-CoA (step AN or AJ). Propylene is formed from butyrate by an alkene-forming decarboxylase (step AK). Pathways from malonyl-ACP to propylene are listed in the table below. 5 Exemplary pathways from shown in Figure 7 are listed in the table below: Product Pathways 1,3-BDO A, D, E, F, G, S A, D, K, S AS, E, F, G, S A, D, E, F, R, AA A, H, F, G, S AS, I, G, S A, D, E, Q, Z, AA A, H, F, R, AA AS, K,, S A, D, P, Y, Z, AA A, H, Q, Z, AA AS, I, R, AA A, D, P, O A, J, G, S AS, E, F, R, AA A, D, E, F, G, S A, J, R, AA AS, E, Q, Z, AA A, D, E, F, R, AA A, B, X, Y, Z, AA AS, P, N, AA A, D, P, N, AA A, B, X, O AS, P, Y, Z, AA A, D, I, G, S A, B, X, N, AA AS, P, O A, D, I R, AA A, B, L, Z, AA AS, E, F, R, AA A, B, M, AA AS, E, F, G, S Crotyl A, B, C, AE, AF, AG, AH A, D, P, AB, AF, AG, AH A, H, Q, Z, AD, AH alcohol A, B, C, AE, W A, D, P, AB, V, AH A, J, R, AD, AH A, B, C, AE, V, AH A, D, P, AB, W AS, I, R, AD, AH A, B, C, T, AG, AH A, D, P, Y, AC, AG, AH AS, E, F, R, AD, AH A, B, C, U, AH A,D, P, Y, Z, AD, AH AS, E, Q, Z, AD, AH A, B, X, Y, Z, AD, AH A, D, P, N, AD, AH AS, E, Q, AC, AG, A, B, X, Y, AC, AG, AH A, D, E, F, R, AD, AH AH A, B, X, AB, AF, AG, AH A, D, E, Q, Z, AD, AH AS, P, N, AD, AH A, B, X, AB, V, AH A, D, E, Q, AC, AG, AH AS, P, Y, Z, AD, AH A, B, X, AB, W A, D, I, R, AD, AH AS, P, Y, AC, AG, AH A, B, L, Z, AD, AH A, H, F, R, AD, AH AS, P, AB, V, AH A, B, L, AC, AG, AH A, H, Q, AC, AG, AH AS, P, AB, AF, AG, A, B, M, AD, AH AH AS, P, AB, W Propylene A, B, C, AL, AI, AJ, AK A, B, L, AC, AG, AO A, H, Q, AR A, B, C, AL, AP, AK A, B, L, AC, AN, AK A, H, Q, Z, AD, AO A, B, C, AE, AF, AG, AO A, B, L, AC, AQ A, H, Q, AC, AQ A, B, C, AE, AF, AQ A, B, M, AD, AO A, H, Q, AC, AG, AO A, B, C, AE, AF, AN, AK A, D, E, F, R, AD, AO A, H, Q, AC, AN, AK A, B, C, AE, AM AJ, AK A, D, E, Q, AR A, H, Q, V, AG, AO A, B, C, AE, V, AO A, D, E, Q, Z, AD, AO AS, I, R, AD, AO A, B, C, T, AG, AO A, D, E, Q, AC, AN, AK AS, E, F, R, AD, AO A, B, C, T, AQ A, D, E, Q, AC, AG, AO AS, E, Q, AD, AO A, B, C, T, AN, AK A, D, E, Q, AC, AQ AS, P, Y, Z, AD, AO A, B, C, U, AO A, D, P, Y, Z, AD, AO AS, P, N, AD, AO A, B, X, Y, Z, AD, AO A, D, P, N, AD, AO AS, E, Q, AC, AG, A, B, X, Y, AR A, D, P, Y, AR AO A, B, X, Y, AC, AN, AK A, D, P, Y, AC, AG, AO AS, P, Y, AC, AG, AO A, B, X, Y, AC, AQ A, D, P, Y, AC, AQ AS, P, AB, AF, AG, A, B, X, Y, AC, AG, AO A, D, P, Y, AC, AN, AK AO A, B, X, N, AD, AO A, D, P, AB, AM, AJ, AK AS, E, Q, AR A, B, X, AB, AF, AG, AO A, D, P, AB, AF, AG, AO AS, P, Y, AR A, B, X, AB, AF, AQ A, D, P, AB, AF, AQ AS, E, Q, AC, AQ A, B, X, AB, AF, AN, AK A, D, P, AB, AF, AN, AK AS, P, Y, AC, AQ A, B, X, AB, AM, AJ, AK A, D, P, AB, V, AO AS, P, AB, AF, AQ A, B, X, AB, V, AO A, D, I, R, AD, AO AS, E, Q, AC, AN, A, B, L, AR A, J, R, AD, AO AK A, B, L, Z, AD, AO A, H, F, R, AD, AO AS, P, Y, AC, AN, AK 201 Product Pathways AS, P, AB, AF, AN, AK AS, P, AB, AM, AJ, AK Enzyme activities required for the reactions shown in Figure 7 are listed in the table below. Label Function Step 7B, 7G, 7P, 7Q, 7R, 7S, 7AA, 1.1.1.a Oxidoreductase (oxo to alcohol) 7AH 1.1.1 .c Oxidoreductase (acyl-CoA to alcohol) 7K, 70, 7W 1.2.1 .b Oxidoreductase (acyl-CoA to aldehyde) 71, 7N, 7V 1.2.1 .e Oxidoreductase (acid to aldehyde) 7F, 7Z, 7AG 1.2.1 .f Oxidoreductase (acyl-ACP to aldehyde) 7J, 7M, 7U 1.3.1 .a Oxidoreducatse (alkane to alkene) 7AL, 7AM, 7AN Acyl-ACP C-acyltransferase 7A 2.3. .e (decarboxylating) 2.3.1 .f CoA-ACP acyltransferase 7D, 7X, 7AE, 7AI 2.3.1 .g Fatty-acid synthase 7A, 7B, 7C, 7AL 2.8.3.a CoA transferase 7E, 7Y, 7AJ, 7AF 3.1.2.a CoA hydrolase 7E, 7Y, 7AJ, 7AF 3.1.2.b Acyl-ACP thioesterase 7H, 7L, 7T, 7AP 4.1.1 .a Decarboxylase 7AQ, 7AR 4.1.1 .b Decarboxylase, alkene forming 7AK 4.1.99.a Decarbonylase 7AO 4.2.1.a Hydro-lyase 7C, 7AB, 7AC, 7AD 6.2.1 .a CoA synthetase 7E, 7Y, 7AJ, 7AF 5 Enzyme candidates in many of these EC classes have been described earlier and represent suitable candidates for to the transformations depicted in Figure 7. These enzyme classes include EC 1.1.1.a, 1.1.l.c, 1.2.1.b, 1.2.1.e, 2.3.1.b, 2.3.1.h, 2.8.3.a, 3.1.2.a, 4.1.1.a, 4.1.99.a, 4.2.1 .a and 6.2.1 .a. New enzyme candidates relevant to the Figure 7 pathways are described below. 10 1.1.1.a Oxidoreductase (oxo to alcohol) Several reactions shown in Figure 7 are catalyzed by alcohol dehydrogenase enzymes. These reactions include Steps B, G, P, Q, R, S, AA and AH. Exemplary alcohol dehydrogenase enzymes for catalyzing steps G, P, Q, R, S, AA and AH were described above in Example VII. Enzyme candidates suitable for catalyzing step B are described 15 below.

202 The reduction of acetoacetyl-ACP to 3-hydroxyacetyl-ACP is catalyzed by acetoacetyl ACP reductase or 3-oxoacyl-ACP reductase (EC 1.1.1.100). The E. coli 3-oxoacyl-ACP reductase is encoded byfabG. Key residues responsible for binding the acyl-ACP 5 substrate to the enzyme have been elucidated (Zhang et al, JBiol Chem 278:52935-43 (2003)). Additional enzymes with this activity have been characterized in Bacillus anthracis (Zaccai et al, Prot Struct Funct Gen 70:562-7 (2008)) and Mycobacterium tuberculosis (Gurvitz, Mol Genet Genomics 282:407-16 (2009)). The beta-ketoacyl reductase (KR) domain of eukaryotic fatty acid synthase also catalyzes this activity 10 (Smith, FASEB J, 8:1248-59 (1994)). Protein GenBank ID GI Number r fabG POAEK2.1 84028081 Escherichia coli fabG AAP27717.1 30258498 Bacillus anthracis FabGl NP 215999.1 15608621 Mycobacterium tuberculosis FabG4 YP 003030167.1 253797166 Mycobacterium tuberculosis 1.2.1.f Oxidoreductase (acvl-ACP to aldehyde) The reduction of an acyl-ACP to its corresponding aldehyde is catalyzed by an acyl-ACP reductase (AAR). Such a transformation is depicted in steps J, M and U of Figure 7. 15 Suitable enzyme candidates include the orfl 594 gene product of Synechococcus elongatus PCC7942 and homologs thereof (Schirmer et al, Science, 329: 559-62 (2010)). The S. elongates PCC7942 acyl-ACP reductase is coexpressed with an aldehyde decarbonylase in an operon that appears to be conserved in a majority of cyanobacterial organisms. This enzyme, expressed in E. coli together with the aldehyde decarbonylase, conferred the 20 ability to produce alkanes. The P. marinus AAR was also cloned into E. coli and, together with a decarbonylase, demonstrated to produce alkanes (US Application 2011/0207203). Protein GenBank ID GI Number Organism orf1 594 YP_400611.1 81300403 Synechococcus elongatus PCC7942 PMT9312_0533 YP_397030.1 78778918 Prochlorococcus marinus MIT 9312 syc0051_d YP_170761.1 56750060 Synechococcus elongatus PCC 6301 Ava 2534 YP 323044.1 75908748 Anabaena variabilis A TCC 29413 alr5284 NP 489324.1 17232776 Nostoc sp. PCC 7120 Aazo 3370 YP 003722151.1 298491974 Nostoc azollae Cyan7425 0399 YP 002481152.1 220905841 Cyanothece sp. PCC 7425 N9414 21225 ZP 01628095.1 119508943 Nodularia spumigena CCY9414 L8106 07064 ZP 01619574.1 119485189 Lyngbya sp. PCC 8106 203 1.3.1.a (alkane to alkene) Several transformations in Figure 7 involve the reduction of an alkene to an alkane, In steps AM and AN, an enoyl-CoA is reduced to its corresponding acyl-CoA. Enzyme 5 candidates for catalyzing these reactions were described previously in Example VII. Step AL depicts the reduction of crotonyl-ACP to butyryl-ACP, catalyzed by a butyryl-ACP reductase. Suitable enzyme candidates for this step are described here. Enoyl-ACP reductase catalyzes the formation of a saturated acyl-ACP by an NAD(P)H dependent reduction of the enoyl-ACP double bond. The FabI protein of E. coli is a well 10 characterized enoyl-ACP reductase that catalyzes the reduction of enoyl substrates of length 4 to 16 carbons (Rafi et al, JBC 281:39285-93 (2006)). FabI is inhibited by acyl ACP by product inhibition (Heath, JBiol Chem 271:1833-6 (1996)). Bacillus subtilis contains two enoyl-ACP reductase isozymes, FabI and FabL (Heath et al, JBiol Chem 275:40128-33 (2000)). The Streptococcus pneumoniae FabK protein is a triclosan 15 resistant flavoprotein catalyzing the same activity (Heath and Rock, Nature 406:145-6 (2000)). An additional candidate is the Pseudomonas aeruginosa FabI protein, which was recently crystallized (Lee et al, Acta Cryst Sect F 67:214-216 (2011)). Protein GenBank ID GI Number Organism fabI POAEK4.2 84028072 Escherichia coli fabI P54616.2 7531269 Bacillus subtilis fabL P71079.1 81817482 Bacillus subtilis fabK AAF98273.1 9789231 Streptococcus pneumoniae fabI Q9ZFE4.1 7531118 Pseudomonas aeruginosa 2.3.1.e Acyl-ACP C-acyltransferase (decarboxylating) 20 In step A of Figure 7, acetoacetyl-ACP is formed from malonyl-ACP and either acetyl CoA or acetyl-ACP. This reaction is catalyzed by an acyl-ACP C-acyltransferase in EC class 2.3.1. The condensation of malonyl-ACP and acetyl-CoA is catalyzed by beta ketoacyl-ACP synthase (KAS, EC 2.3.1.180). E, coli has three KAS enzymes encoded by 25 fabB,fabF andfabH. FabH (KAS III), the key enzyme of initiation of fatty acid biosynthesis in E. coli, is selective for the formation of acetoacetyl-ACP. FabB and FabF catalyze the condensation of malonyl-ACP with acyl-ACP substrates and function primarily in fatty acid elongation although they can also react with acetyl-ACP and thereby participate in fatty acid inititation. For example, the Bacillus subtilis KAS 204 enzymes are similar to FabH but are less selective, accepting branched acyl-CoA substrates (Choi et al, JBacteriol 182:365-70 (2000)). Protein GenBank ID GI Number Organism fabB AAC75383.1 1788663 Escherichia coli fabF AAC74179.1 1787337 Escherichia coli fabH AAC74175.1 1787333 Escherichia coli FabHA NP 389015.1 16078198 Bacillus subtilis FabHB NP 388898.1 16078081 Bacillus subtilis Alternately, acetyl-CoA can first be activated to acetyl-ACP and subsequently condensed 5 to acetoacetyl-ACP by two enzymes, acetyl-CoA:ACP transacylase (EC 2.3.1.38) and acetoacetyl-ACP synthase (EC 2.3.1.41). Acetyl-CoA:ACP transacylase converts acetyl CoA and an acyl carrier protein to acetyl-ACP, releasing CoA. Enzyme candidates for acetyl-CoA:ACP transacylase are described in section EC 2.3.1.f below. Acetoacetyl-ACP synthase enzymes catalyze the condensation of acetyl-ACP and malonyl-ACP. This 10 activity is catalyzed by FabF and FabB of E. coli, as well as the multifunctional eukaryotic fatty acid synthase enzyme complexes described in EC 2.3.1.g. 2.3.1.f CoA-ACP acyltransferase The exchange of an ACP moiety for a CoA is catalyzed by enzymes in EC class 2.3.1. 15 This reaction is shown in steps D, X, AE and Al of Figure 7. Activation of acetyl-CoA to acetyl-ACP (step A of Figure 7) is also catalyzed by a CoA:ACP acyltransferase. Enzymes with CoA-ACP acyltransferase activity include acetyl-CoA:ACP transacylase (EC 2.3.1.38) and malonyl-CoA:ACP transacylase (EC 2.3.1.39). The FabH (KASHI) enzyme of E. coli functions as an acyl-CoA:ACP transacylase, in 20 addition to its primary activity of forming acetoacetyl-ACP. Butyryl-ACP is accepted as an alternate substrate of FabH (Prescott et al, Adv. Enzymol. Relat. Areas Mol, 36:269 311 (1972)). Acetyl-CoA:ACP transacylase enzymes from Plasmodium falciparum and Streptomyces avermitillis have been heterologously expressed in E. coli (Lobo et al, Biochem 40:11955-64 (2001)). A synthetic KASIII (FabH) from P. falciparum expressed 25 in a fabH-deficient Lactococcus lactis host was able to complement the native fadH activity (Du et al, AEM 76:3959-66 (2010)). The acetyl-CoA:ACP transacylase enzyme from Spinacia oleracea accepts other acyl-ACP molecules as substrates, including butyryl ACP (Shimakata et al, Methods Enzym 122:53-9 (1986)). The sequence of this enzyme has not been determined to date. Malonyl-CoA:ACP transacylase enzymes include FabD 205 of E. coli and Brassica napsus (Verwoert et al, J Bacteriol, 174:2851-7 (1992); Simon et al, FEBS Lett 435:204-6 (1998)). FabD of B. napsus was able to complementfabD deficient E. coli. The multifunctional eukaryotic fatty acid synthase enzyme complexes (described in EC 2.3.1 .g) also catalyze this activity. Protein GenBank ID GI Number Organism fabH AAC74175.1 1787333 Escherichia coli fadA NP 824032.1 29829398 Streptomyces avermitillis fabH AAC63960.1 3746429 Plasmodium falciparum Synthetic construct ACX34097.1 260178848 Plasmodiumfalciparum fabH CAL98359.1 124493385 Lactococcus lactis fabD AAC74176.1 1787334 Escherichia coli fabD CAB45522.1 5139348 Brassica napsus 5 2.3.1.2 Fatty acid synthase Steps A, B, C and AL of Figure 7 can together be catalyzed fatty acid synthase or fatty acyl-CoA synthase, multifunctional enzyme complexes composed of multiple copies of 10 one or more subunits. The fatty acid synthase of Saccharomyces cerevisiae is a dodecamer composed of two multifunctional subunits FASI and FAS2 that together catalyze all the reactions required for fatty acid synthesis: activation, priming, elongation and termination (Lomakin et al, Cell 129:319-32 (2007)). This enzyme complex catalyzes the formation of long chain fatty acids from acetyl-CoA and malonyl-CoA. The favored product of 15 eukaryotic FAS systems is palmitic acid (C 16). Similar fatty acid synthase complexes are found in Candida parapsilosis and Thermomyces lanuginosus (Nguyen et al, PLoS One 22:e8421 (2009); Jenni et al, Science 316:254-61 (2007)). The multifunctional Fas enzymes of Mycobacterium tuberculosis and mammals such as Homo sapiens are also suitable candidates (Fernandes and Kolattukudy, Gene 170:95-99 (1996) and Smith et al, 20 Prog Lipid Res 42:289-317 (2003)). Protein GenBank ID GI Number Organism FASI CAA82025.1 486321 Saccharomyces cerevisiae FAS2 CAA97948.1 1370478 Saccharomyces cerevisiae Fas1 ABO37973.1 133751597 Thermomyces lanuginosus Fas2 ABO37974.1 133751599 Thermomyces lanuginosus Fas AAB03809.1 1036835 Mycobacterium tuberculosis Fas NP 004095.4 41872631 Homo sapiens 206 3.1.2.b Acyl-ACP thioesterase Acyl-ACP thioesterase enzymes convert an acyl-ACP to its corresponding acid. Such a transformation is required in steps H, L, T and AP of Figure 7. Exemplary enzymes include the FatA and FatB isoforms of Arabidopsis thaliana (Salas et al, Arch Biochem 5 Biophys 403:25-34 (2002)). The activities of these two proteins vary with carbon chain length, with FatA preferring oleyl-ACP and FatB preferring palmitoyl-ACP. , See 3.1.2.14. A number of thioesterases with different chain length specificities are listed in WO 2008/113041 and are included in the table below [see p 126 Table 2A of patent]. For example, it has been shown previously that expression of medium chain plant thioesterases 10 like FatB from Umbellularia californica in E. coli results in accumulation of high levels of medium chain fatty acids, primarily laurate (C 12:0). Similarly, expression of Cuphea palustris FatB 1 thioesterase in E. coli led to accumulation of C8-10:0 acyl-ACPs (Dehesh et al, Plant Physiol 110:203-10 (1996)). Similarly, Carthamus tinctorius thioesterase, when expressed in E. coli leads to >50 fold elevation in C 18:1 chain termination and 15 release as free fatty acid (Knutzon et al, Plant Physiol 100:1751-58 (1992)). Methods for altering the substrate specificity of acyl-ACP thioesterases are also known in the art (for example, EP 1605048). Protein GenBank ID GI Number Organism fatA AEE76980.1 332643459 Arabidopsis thaliana fatB AEE28300.1 332190179 Arabidopsis thaliana fatB2 AAC49269.1 1292906 Cuphea hookeriana fatBI AAC491791 1215718 Cuphea palustris M96568.1:94..1251 AAA33019.1 404026 Carthamus tinctorius fatBI Q41635.1 8469218 Umbellularia californica tesA AAC73596.1 1786702 Escherichia coli 4.1.99.a Decarbonylase 20 Decarbonylase enzyme candidates described in Example VII are also relevant here. Additional enzyme candidates suitable for catalyzing decarbonylation reactions in Figures 1-7 include the orfi593 gene product of Synechococcus elongatus PCC7942 and homologs thereof (US Application 2011/0207203). Protein GenBank ID GI Number Organism Orf 1593 YP 400610.1 81300402 Synechococcus etongatus PCC7942 25 207 4.2.1.a Hydro-ivase Several reactions in Figure 7 depict dehydration reactions, including steps C, AB, AC and AD. Candidate hydro-lyase enzymes described in Example VII are also applicable here. Oleate hydratase enzymes are applicable to catalyze all the dehydration reactions in 5 Figures 1-7, in particular the dehydration of 3-buten-1-ol to butadiene. Oleate hydratase enzymes catalyze the reversible hydration of non-activated atkenes to their corresponding alcohols. These enzymes represent additional suitable candidates as suggested in W02011076691. Oleate hydratases from Elizabethkingia meningoseptica and Streptococcus pyogenes have been characterized (WO 2008/119735). Examples include 10 the following proteins. Protein GenBank ID GI Number Organism OhyA ACT54545.1 254031735 Elizabethkingia meningoseptica HMPREF08411446 ZP_07461147.1 306827879 Streptococcus pyogenes ATCC 10782 P700755_13397 ZP_01252267.1 91215295 Psychroflexus torquis ATCC 700755 RPB_2430 YP_486046.1 86749550 Rhodopseudomonas palustris 3-Hydroxyacyl-ACP dehydratase enzymes are suitable candidates for dehydrating 3 hydroxybutyryl-ACP to crotonyl-ACP (step C of Figure 7). Enzymes with this activity include FabA and FabZ of E. coli, which posess overlapping broad substrate specificities 15 (Heath, JBiol Chem 271:1833-6 (1996)). Fatty acid synthase complexes, described above, also catalyze this reaction. The FabZ protein from Plasmodiumfalciparum has been crystallized (Kostrew et al, Protein Sci 14:1570-80 (2005)). Additional candidates are the mitochondrial 3-hydroxyacyl-ACP dehydratase encoded by Htd2p in yeast and TbHTD2 in Homo sapiens and Trypanosoma brucei (Kastanoitis et al, Mol Micro 53:1407-21 20 (2004); Kaija et al, FEBS Lett 582:729-33 (2008)). Protein GenBank ID GI Number Organism fA AAC74040.1 1787187 Escherichia coli fabZ AAC73291.1 1786377 Escherichia coli PfFabZ AAK83685.1 15080870 Plasmodiumfalciparum Htd2p NP 011934.1 6321858 Saccharomyces cerevisiae HTD2 P86397.1 281312149 Homo sapiens . 208 EXAMPLE XIV Chemical Production of Butadiene From Crotyl Alcohol In a typical process for converting crotyl alcohol into butadiene, crotyl alcohol is passed, 5 either neat or in a solvent and either in presence or absence of steam, over a solid inorganic, organic or metal-containing dehydration catalyst heated to temperatures in the range 40-400 "C inside of the reaction vessel or tube, leading to elimination of water and release of butadiene as a gas, which is condensed (butadiene bp = -4.4"C) and collected in a reservoir for further processing, storage, or use. Typical catalysts can include bismuth 10 molybdate, phosphate-phosphoric acid, cerium oxide, kaolin-iron oxide, kaolin-phosphoric acid, silica-alumina, and alumina. Typical process throughputs are in the range of 0.1 20,000 kg/h. Typical solvents are toluene, heptane, octane, ethylbenzene, and xylene. EXAMPLE XV 15 Enzymatic pathways for Producing Butadiene from Crotyl Alcohol This example describes enzymatic pathways for converting crotyl alcohol to butadiene. The two pathways are shown in Figure 12. In one pathway, crotyl alcohol is phosphorylated to 2-butenyl-4-phosphate by a crotyl alcohol kinase (Step A). The 2 20 butenyl-4-phosphate intermediate is again phosphorylated to 2-butenyl-4-diphosphate (Step B). A butadiene synthase enzyme catalyzes the conversion of 2-butenyl-4 diphosphate to butadiene (Step C). Such a butadiene synthase can be derived from a phosphate lyase enzyme such as isoprene synthase using methods, such as directed evolution, as described herein. In an alternate pathway, crotyl alcohol is directly 25 converted to 2-butenyl-4-diphosphate by a diphosphokinase (step D). Enzyme candidates for steps A-D are provided below. Crotyl alcohol kinase (Figure 12, Step A) Crotyl alcohol kinase enzymes catalyze the transfer of a phosphate group to the hydroxyl 30 group of crotyl alcohol. The enzymes described below naturally possess such activity or can be engineered to exhibit this activity. Kinases that catalyze transfer of a phosphate group to an alcohol group are members of the EC 2.7.1 enzyme class. The table below lists several useful kinase enzymes in the EC 2.7.1 enzyme class.

209 Enzyme Enzyme Enzyme Commission Commission Commission Number Enzyme Name Number Enzyme Name Number Enzyme Name 2.7.1.1 hexokinase 2.7.1.48 uridine kinase 2.7.1.94 acylglycerol kinase hydroxymethylpyrimidine 2.7.1.2 glucokinase 2.7.1.49 kinase 2.7.1.95 kanamycin kinase 2.7.1.3 ketohexokinase 2.7.1.50 hydroxyethylthiazole kinase 2.7.1.100 S-methyl-5-thioribose kinase 2.7.1.4 fructokinase 2.7.1.51 L-fuculokinase 2.7.1.101 tagatose kinase 2.7.1.5 rhamnulokinase 2.7.1.52 fucokinase 2.7.1.102 hamamelose kinase 2.7.1.6 galactokinase 2.7.1.53 L-xylulokinase 2.7.1.103 viomycin kinase 2.7.1.7 mannokinase 2.7.1.54 D-arabinokinase 2.7.1.105 6-phosphofructo-2-kinase glucose-1,6-bisphosphate 2.7.1.8 glucosamine kinase 2.7.1.55 allose kinase 2.7.1.106 synthase 2.7.1.10 phosphoglucokinase 2.7.1.56 1 -phosphofructokinase 2.7.1.107 diacylglycerol kinase 2-dehydro-3 2.7.1.11 6-phosphofructokinase 2.7.1.58 deoxygalactonokinase 2.7.1.108 dolichol kinase 2.7.1.12 gluconokinase 2.7.1.59 N-acetylglucosamine kinase 2.7.1.113 deoxyguanosine kinase 2.7.1.13 dehydrogluconokinase 2.7.1.60 N-acylmannosamine kinase 2.7.1.114 AMP-thymidine kinase acyl-phosphate-hexose 2.7.1.14 sedoheptulokinase 2.7.1.61 phosphotransferase 2.7.1.118 ADP-thymidine kinase phosphoramidate-hexose 2.7.1.15 ribokinase 2.7.1.62 phosphotransferase 2.7.1.119 hygromycin-B 7"-O-kinase polyphosphate-glucose phosphoenolpyruvate 2.7.1.16 ribulokinase 2.7.1.63 phosphotransferase 2.7.1.121 glycerone phosphotransferase 2.7.1.17 xylulokinase 2.7.1.64 inositol 3-kinase 2.7.1.122 xylitol kinase inositol-trisphosphate 3 2.7.1.18 phosphoribokinase 2.7.1.65 scyllo-inosamine 4-kinase 2.7.1.127 kinase 2.7.1.19 phosphoribulokinase 2.7.1.66 undecaprenol kinase 2.7.1.130 tetraacyldisaccharide 4'-kinase 210 Enzyme Enzyme Enzyme Commission Commission Commission Number Enzyme Name Number Enzyme Name Number Enzyme Name inositol-tetrakisphosphate 1 2.7.1.20 adenosine kinase 2.7.1.67 1 -phosphatidylinositol 4-kinase 2.7.1.134 kinase 1 -phosphatidylinositol-4 2.7.1.21 thymidine kinase 2.7.1.68 phosphate 5-kinase 2.7.1.136 macrolide 2'-kinase ribosylnicotinamide protein-Np-phosphohistidine 2.7.1.22 kinase 2.7.1.69 sugar phosphotransferase 2.7.1.137 phosphatidylinositol 3-kinase 2.7.1.23 NAD+ kinase 2.7.1.70 identical to EC 2.7.1.37. 2.7.1.138 ceramide kinase inositol-tetrakisphosphate 5 2.7.1.24 dephospho-CoA kinase 2.7.1.71 shikimate kinase 2.7.1.140 kinase glycerol-3-phosphate 2.7.1.25 adenylyl-sulfate kinase 2.7.1.72 streptomycin 6-kinase 2.7.1.142 glucose phosphotransferase diphosphate-purine nucleoside 2.7.1.26 riboflavin kinase 2.7.1.73 inosine kinase 2.7.1.143 kinase 2.7.1.27 erythritol kinase 2.7.1.74 deoxycytidine kinase 2.7.1.144 tagatose-6-phosphate kinase 2.7.1.28 triokinase 2.7.1.76 deoxyadenosine kinase 2.7.1.145 deoxynucleoside kinase ADP-dependent 2.7.1.29 glycerone kinase 2.7.1.77 nucleoside phosphotransferase 2.7.1.146 phosphofructokinase polynucleotide 5'-hydroxyl 2.7.1.30 glycerol kinase 2.7.1.78 kinase 2.7.1.147 ADP-dependent glucokinase diphosphate-glycerol 4-(cytidine 5'-diphospho)-2 2.7.1.31 glycerate kinase 2.7.1.79 phosphotransferase 2.7.1.148 C-methyl-D-erythritol kinase diphosphate-serine I-phosphatidylinositol-5 2.7.1.32 choline kinase 2.7.1.80 phosphotransferase 2.7.1.149 phosphate 4-kinase 1-phosphatidylinositol-3 2.7.1.33 pantothenate kinase 2.7.1.81 hydroxylysine kinase 2.7.1.150 phosphate 5-kinase inositol-polyphosphate 2.7.1.34 pantetheine kinase 2.7.1.82 ethanolamine kinase 2.7.1.151 multikinase 211 Enzyme Enzyme Enzyme Commission Commission Commission Number Enzyme Name Number Enzyme Name Number Enzyme Name phosphatidylinositol-4,5 2.7.1.35 pyridoxal kinase 2.7.1.83 pseudouridine kinase 2.7.1.153 bisphosphate 3-kinase phosphatidylinositol-4 2.7.1.36 mevalonate kinase 2.7.1.84 alkylglycerone kinase 2.7.1.154 phosphate 3-kinase 2.7.1.39 homoserine kinase 2.7.1.85 8-glucoside kinase 2.7.1.156 adenosylcobinamide kinase 2.7.1.40 pyruvate kinase 2.7.1.86 NADH kinase 2.7.1.157 N-acetylgalactosamine kinase glucose-i-phosphate inositol-pentakisphosphate 2 2.7.1.41 phosphodismutase 2.7.1.87 streptomycin 3"-kinase 2.7.1.158 kinase riboflavin dihydrostreptomycin-6- inositol-1,3,4-trisphosphate 2.7.1.42 phosphotransferase 2.7.1.88 phosphate 3'a-kinase 2.7.1.159 5/6-kinase 2.7.1.43 glucuronokinase 2.7.1.89 thiamine kinase 2.7.1.160 2'-phosphotransferase diphosphate-fructose-6- CTP-dependent riboflavin 2.7.1.44 galacturonokinase 2.7.1.90 phosphate 1 -phosphotransferase 2.7.1.161 kinase 2-dehydro-3 2.7.1.45 deoxygluconokinase 2.7.1.91 sphinganine kinase 2.7.1.162 N-acetylhexosamine 1-kinase 5-dehydro-2 2.7.1.46 L-arabinokinase 2.7.1.92 deoxygluconokinase 2.7.1.163 hygromycin B 4-0-kinase O-phosphoseryl-tRNASec 2.7.1.47 D-ribulokinase 2.7.1.93 alkylglycerol kinase 2.7.1.164 kinase 212 Mevalonate kinase (EC 2.7.1.36) phosphorylates the terminal hydroxyl group of mevalonate. Gene candidates for this step include erg 12 from S. cerevisiae, mvk from Methanocaldococcusjannaschi, MVK from Homo sapeins, and mvk from Arabidopsis thaliana col. Additional mevalonate kinase candidates include the feedback-resistant 5 mevalonate kinase from the archeon Methanosarcina mazei (Primak et al, AEM, in press (2011)) and the Mvk protein from Streptococcus pneumoniae (Andreassi et al, Protein Sci, 16:983-9 (2007)). Mvk proteins from S. cerevisiae, S. pneumoniae and M. mazei were heterologously expressed and characterized in E. coli (Primak et al, supra). The S. pneumoniae mevalonate kinase was active on several alternate substrates including 10 cylopropylmevalonate, vinylmevalonate and ethynylmevalonate (Kudoh et al, Bioorg Med Chem 18:1124-34 (2010)), and a subsequent study determined that the ligand binding site is selective for compact, electron-rich C(3)-substituents (Lefurgy et al, JBiol Chem 285:20654-63 (2010)). Protein GenBank ID GI Number Organism erg12 CAA39359.1 3684 Sachharomyces cerevisiae mvk Q58487.1 2497517 Methanocaldococcus jannaschii mvk AAH16140.1 16359371 Homo sapiens mvk NP_851084.1 30690651 Arabidopsis thaliana mvk NP 633786.1 21227864 Methanosarcina mazei mvk NP 357932.1 15902382 Streptococcus pneumoniae 15 Glycerol kinase also phosphorylates the terminal hydroxyl group in glycerol to form glycerol-3-phosphate. This reaction occurs in several species, including Escherichia coli, Saccharomyces cerevisiae, and Thermotoga maritima. The E. coli glycerol kinase has been shown to accept alternate substrates such as dihydroxyacetone and glyceraldehyde (Hayashi et al., JBiol.Chem. 242:1030-1035 (1967)). T, maritime has two glycerol kinases 20 (Nelson et al., Nature 399:323-329 (1999)). Glycerol kinases have been shown to have a wide range of substrate specificity. Crans and Whiteside studied glycerol kinases from four different organisms (Escherichia coli, S. cerevisiae, Bacillus stearothermophilus, and Candida mycoderma) (Crans et al., J.Am.Chem.Soc. 107:7008-7018 (2010); Nelson et al., supra, (1999)). They studied 66 different analogs of glycerol and concluded that the 25 enzyme could accept a range of substituents in place of one terminal hydroxyl group and that the hydrogen atom at C2 could be replaced by a methyl group. Interestingly, the kinetic constants of the enzyme from all four organisms were very similar.

213 Protein GenBank ID GI Number Organism glpK AP_003883.1 89110103 Escherichia coli K12 glpK1 NP 228760.1 15642775 Thermotoga maritime MSB8 gIpK2 NP 229230.1 15642775 Thermotoga maritime MSB8 Gut] NP 011831.1 82795252 Saccharomyces cerevisiae Homoserine kinase is another possible candidate. This enzyme is also present in a number of organisms including E. coli, Streptomyces sp, and S. cerevisiae. Homoserine kinase 5 from E. coli has been shown to have activity on numerous substrates, including, L-2 amino,1,4- butanediol, aspartate semialdehyde, and 2-amino-5-hydroxyvalerate (Huo et al., Biochemistry 35:16180-16185 (1996); Huo et al., Arch.Biochem.Biophys. 330:373-379 (1996)). This enzyme can act on substrates where the carboxyl group at the alpha position has been replaced by an ester or by a hydroxymethyl group. The gene candidates are: Protein GenBank ID GI Number Organism thrB BAB96580.2 85674277 Escherichia coli K12 SACTIDRAFT_4809 ZP_06280784.1 282871792 Streptomyces sp. A CT-1 Thr1 AAA35154.1 172978 Saccharomyces serevisiae 10 2-Butenvl-4-phosphate kinase (Figure 12, Step B) 2-Butenyl-4-phosphate kinase enzymes catalyze the transfer of a phosphate group to the phosphate group of 2-butenyl-4-phosphate. The enzymes described below naturally 15 possess such activity or can be engineered to exhibit this activity. Kinases that catalyze transfer of a phosphate group to another phosphate group are members of the EC 2.7.4 enzyme class. The table below lists several useful kinase enzymes in the EC 2.7.4 enzyme class. Enzyme Commission Number Enzyme Name 2.7.4.1 polyphosphate kinase 2.7.4.2 phosphomevalonate kinase 2.7.4.3 adenylate kinase 2.7.4.4 nucleoside-phosphate kinase 2.7.4.6 nucleoside-diphosphate kinase 2.7.4.7 phosphomethylpyrimidine kinase 2.7.4.8 guanylate kinase 2.7.4.9 dTMP kinase 2.7.4.10 nucleoside-triphosphate-adenylate kinase 2.7.4.11 (deoxy)adenylate kinase 214 Enzyme Commission Number Enzyme Name 2.7.4.12 T2-induced deoxynucleotide kinase 2.7.4.13 (deoxy)nucleoside-phosphate kinase 2.7.4.14 cytidylate kinase 2.7.4.15 thiamine-diphosphate kinase 2.7.4.16 thiamine-phosphate kinase 3-phosphoglyceroyl-phosphate 2.7.4.17 polyphosphate phosphotransferase 2.7.4.18 farnesyl-diphosphate kinase 2.7.4.19 5-methyldeoxycytidine-5'-phosphate kinase dolichyl-diphosphate--polyphosphate 2.7.4.20 phosphotransferase 2.7.4.21 inositol-hexakisphosphate kinase 2.7.4.22 UMP kinase 2.7.4.23 ribose 1,5-bisphosphate phosphokinase 2.7.4.24 diphosphoinositol-pentakisphosphate kinase 2.7.4.- Farnesyl monophosphate kinase 2.7.4.- Geranyl-geranyl monophosphate kinase 2.7.4.- Phytyl-phosphate kinase Phosphomevalonate kinase enzymes are of particular interest. Phosphomevalonate kinase (EC 2.7.4.2) catalyzes the analogous transformation to 2-butenyl-4-phosphate kinase. This enzyme is encoded by erg8 in Saccharomyces cerevisiae (Tsay et al., Mol. Cell Biol. 5 11:620-631 (1991)) and mvaK2 in Streptococcus pneumoniae, Staphylococcus aureus and Enterococcusfaecalis (Doun et al., Protein Sci. 14:1134-1139 (2005); Wilding et al., J Bacteriol. 182:4319-4327 (2000)). The Streptococcus pneumoniae and Enterococcus faecalis enzymes were cloned and characterized in E. coli (Pilloff et al., J Biol. Chem. 278:4510-4515 (2003); Doun et al., Protein Sci. 14:1134-1139 (2005)). The S. 10 pneumoniae phosphomevalonate kinase was active on several alternate substrates including cylopropylmevalonate phosphate, vinylmevalonate phosphate and ethynylmevalonate phosphate (Kudoh et al, Bioorg Med Chem 18:1124-34 (2010)). Protein GenBank ID GI Number Organism Erg8 AAA34596.1 171479 Saccharomyces cerevisiae mvaK2 AAG02426.1 9937366 Staphylococcus aureus mvaK2 AAG02457.1 9937409 Streptococcus pneumoniae mvaK2 AAG02442.1 9937388 Enterococcusfaecalis Farnesyl monophosphate kinase enzymes catalyze the CTP dependent phosphorylation of 15 farnesyl monophosphate to famesyl diphosphate. Similarly, geranylgeranyl phosphate kinase catalyzes CTP dependent phosphorylation. Enzymes with these activities were 215 identified in the microsomal fraction of cultured Nicotiana tabacum (Thai et al, PNAS 96:13080-5 (1999)). However, the associated genes have not been identified to date. Butadiene synthase (Figure 12, Step C) 5 Butadiene synthase catalyzes-the conversion of 2-butenyl-4-diphosphate to 1,3-butadiene. The enzymes described below naturally possess such activity or can be engineered to exhibit this activity. Carbon-oxygen lyases that operate on phosphates are found in the EC 4.2.3 enzyme class. The table below lists several useful enzymes in EC class 4.2.3. Enzyme Commission Number Enzyme Name 4.2.3.15 Myrcene synthase 4.2.3.26 Linalool synthase 4.2.3.27 Isoprene synthase 4.2.3.36 Terpentriene sythase 4.2.3.46 (E, E)-alpha-Farnesene synthase 4.2.3.47 Beta-Farnesene synthase 4.2.3.49 Nerolidol synthase 10 Particularly useful enzymes include isoprene synthase, myrcene synthase and farnesene synthase. Enzyme candidates are described below. Isoprene synthase naturally catalyzes the conversion of dimethylallyl diphosphate to isoprene, but can also catalyze the synthesis of 1,3-butadiene from 2-butenyl-4 diphosphate. Isoprene synthases can be found in several organisms including Populus alba 15 (Sasaki et al., FEBS Letters, 2005, 579 (11), 2514-2518), Pueraria montana (Lindberg et al., Metabolic Eng, 12(1):70-79 (2010); Sharkey et al., Plant Phvsiol., 137(2):700-712 (2005)), and Populus tremula x Populus alba, also called Populus canescens (Miller et al., Planta, 2001, 213 (3), 483-487). The crystal structure of the Populus canescens isoprene synthase was determined (Koksal et al, J Mol Biol 402:363-373 (2010)). Additional 20 isoprene synthase enzymes are described in (Chotani et al., WO/2010/031079, Systems Using Cell Culture for Production of Isoprene; Cervin et al., US Patent Application 20100003716, Isoprene Synthase Variants for Improved Microbial Production of Isoprene). Protein GenBank ID GI Number Organism ispS BAD98243.1 63108310 Populus alba ispS AAQ84170.1 35187004 Pueraria montana ispS CAC35696.1 13539551 Populus tremula x Populus alba 216 Myrcene synthase enzymes catalyze the dephosphorylation of geranyl diphosphate to beta myrcene (EC 4.2.3.15). Exemplary myrcene synthases are encoded by MST2 of Solanum lycopersicum (van Schie et al, Plant Mol Biol 64:D473-79 (2007)), TPS-Myr of Picea 5 abies (Martin et al, Plant Physiol 135:1908-27 (2004)) g-myr of Abies grandis (Bohlmann et al, J Biol Chem 272:21784-92 (1997)) and TPS10 of Arabidopsis thaliana (Bohlmann et al, Arch Biochem Biophys 375:261-9 (2000)). These enzymes were heterologously expressed in E. coli. Protein GenBank ID GI Number Organism MST2 ACN58229.1 224579303 Solanum lycopersicum TPS-Myr AAS47690.2 77546864 Picea abies G-myr 024474.1 17367921 Abies grandis TPSIO EC07543.1 330252449 Arabidopsis thaliana 10 Farnesyl diphosphate is converted to alpha-farnesene and beta-farnesene by alpha farnesene synthase and beta-farnesene synthase, respectively. Exemplary alpha-farnesene synthase enzymes include TPS03 and TPSO2 of Arabidopsis thaliana (Faldt et al, Planta 216:745-51 (2003); Huang et al, Plant Physiol 153:1293-310 (2010)), afs of Cucumis sativus (Mercke et al, Plant Physiol 135:2012-14 (2004), eafar of Malus x domestica 15 (Green et al, Phytochem 68:176-88 (2007)) and TPS-Far of Picea abies (Martin, supra). An exemplary beta-farnesene synthase enzyme is encoded by TPS1 of Zea mays (Schnee et al, Plant Physiol 130:2049-60 (2002)). Protein GenBank ID GI Number Organism TPS03 A4FVP2.1 205829248 Arabidopsis thaliana TPS02 POCJ43.1 317411866 Arabidopsis thaliana TPS-Far AAS47697.1 44804601 Picea abies afs AAU05951.1 51537953 Cucumis sativus eafar Q84LB2.2 75241161 Malus x domestica TPS1 Q84ZW8.1 75149279 Zea mays Crotvl alcohol diphosphokinase (Figure 12, Step D) 20 Crotyl alcohol diphosphokinase enzymes catalyze the transfer of a diphosphate group to the hydroxyl group of crotyl alcohol. The enzymes described below naturally possess such activity or can be engineered to exhibit this activity. Kinases that catalyze transfer of a diphosphate group are members of the EC 2.7.6 enzyme class. The table below lists 25 several useful kinase enzymes in the EC 2.7.6 enzyme class.

217 Enzyme Commission Number Enzyme Name 2.7.6.1 ribose-phosphate diphosphokinase 2.7.6.2 thiamine diphosphokinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine 2.7.6.3 diphosphokinase 2.7.6.4 nucleotide diphosphokinase 2.7.6.5 GTP diphosphokinase Of particular interest are ribose-phosphate diphosphokinase enzymes which have been identified in Escherichia coli (Hove-Jenson et al., J Biol Chem, 1986, 261(15);6765-71) and Mycoplasma pneumoniae M129 (McElwain et al, International Journal of Systematic 5 Bacteriology, 1988, 38:417-423) as well as thiamine diphosphokinase enzymes. Exemplary thiamine diphosphokinase enzymes are found in Arabidopsis thaliana (Ajjawi, Plant Mol Biol, 2007, 65(1-2);151-62). Protein GenBank ID GI Number Organism prs NP 415725.1 16129170 Escherichia coli prsA NP_109761.1 13507812 Mycoplasma pneumoniae M129 TPKI BAH19964.1 222424006 Arabidopsis thaliana col TPK2 BAH57065.1 227204427 Arabidopsis thaliana col Throughout this application various publications have been referenced. The disclosures of 10 these publications in their entireties, including GenBank and GI number publications, are hereby incorporated by reference in this application in order to more fully describe the state of the art to which this invention pertains. Although the invention has been described with reference to the examples provided above, it should be understood that various modifications can be made without departing from the spirit of the invention. 15 In the claims which follow and in the preceding description of the invention, except where the context requires otherwise due to express language or necessary implication, the word "comprise" or variations such as "comprises" or "comprising" is used in an inclusive sense, i.e. to specify the presence of the stated feature but not to preclude the presence or addition of further features in various embodiments of the invention. It is to be understood that, if any prior art publication is referred to herein, such reference does not constitute an admission that the publication forms a part of the common general knowledge in the art, in Australia or any other country.

Claims (16)

1. A non-naturally occurring microbial organism, a 1,3-butanediol pathway comprising exogenous nucleic acids encoding 1,3-butanediol pathway enzymes expressed in a sufficient amount to produce 1,3-butanediol, wherein said 1,3-butanediol pathway comprises a pathway selected from: (6) 7A, 7D, 7E, 7F, 7G and 7S; (7) 7A, 7D, 71, 7G and 7S; (8) 7A, 7D, 7K, and 7S; (9) 7A, 7H, 7F, 7G and 7S; (10) 7A, 7J, 7G and 7S; (11) 7A, 7J, 7R and 7AA; (12) 7A, 7H, 7F, 7R and 7AA; (13) 7A, 7H, 7Q, 7Z and 7AA; (14) 7A, 7D, 71, 7R and 7AA; (15) 7A, 7D, 7E, 7F, 7R and 7AA; (16) 7A, 7D, 7E, 7Q, 7Z and 7AA; (17) 7A, 7D, 7P, 7N and 7AA; (18) 7A, 7D, 7P, 7Y, 7Z and 7AA; (19) 7A, 7B, 7M and 7AA; (20) 7A, 7B, 7L, 7Z and 7AA; (21) 7A, 7B, 7X, 7N and 7AA; (22) 7A, 7B, 7X, 7Y, 7Z and 7AA; (23) 7A, 7D, 7P and 70; (24) 7A, 7B, 7X and 70; (25) 7A, 7D, 7E, 7F, 7R, 7AA; (26) 7A, 7D, 7E, 7F, 7G, 7S; wherein 7A is a 3-ketoacyl-ACP synthase catalyzing conversion of malonyl-ACP to acetoacetyl-ACP, wherein 7B is an acetoacetyl-ACP reductase catalyzing conversion of acetoacetyl-ACP to 3-hydroxybutyryl-ACP, wherein 7D is an acetoacetyl-CoA:ACP transferase catalyzing conversion of acetoacetyl-ACP to acetoacetyl-CoA, wherein 7E is an acetoacetyl-CoA hydrolase, acetoacetyl-CoA transferase or acetoacetyl-CoA synthetase catalyzing conversion of acetoacetyl-CoA to acetoacetate, wherein 7F is an acetoacetate 219 reductase (acid reducing) catalyzing conversion of acetoacetate to 3-oxobuytraldehyde, wherein 7G is a 3-oxobutyraldehyde reductase (aldehyde reducing) catalyzing conversion of

3-oxobutyraldehyde to 4-hydroxy-2-butanone, wherein 7H is an acetoacetyl-ACP thioesterase catalyzing conversion of acetoacetyl-ACP to acetoacetate, wherein 71 is an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming) catalyzing conversion of acetoacetyl-CoA to 3-oxobuytraldehyde, wherein 7J is an acetoacetyl-ACP reductase (aldehyde forming) catalyzing conversion of acetoacetyl-ACP to 3-oxobutyraldehyde, wherein 7K is an acetoacetyl-CoA reductase (alcohol forming) catalyzing conversion of acetoacetyl-CoA to 4-hydroxy-2-butanone, wherein 7L is a 3-hydroxybutyryl-ACP thioesterase catalyzing conversion of 3-hydroxybutyryl-ACP to 3-hydroxybutyrate, wherein 7M is a 3-hydroxybutyryl-ACP reductase (aldehyde forming) catalyzing conversion of 3 hydroxybutyryl-ACP to 3-hydroxybutyraldehdye, wherein 7N is a 3-hydroxybutyryl-CoA reductase (aldehyde forming) catalyzing conversion of 3-hydroxybutyryl-CoA to 3 hydroxybutyraldehyde, wherein 70 is a 3-hydroxybutyryl-CoA reductase (alcohol forming) catalyzing conversion of 3-hydroxybutyryl-CoA to 1,3-butanediol, wherein 7P is an acetoacetyl-CoA reductase (ketone reducing) catalyzing conversion of acetoacetyl-CoA to 3 hydroxybutyryl-CoA, wherein 7Q is an acetoacetate reductase (ketone reducing) catalyzing conversion of acetoacetate to 3-hydroxybutyrate, wherein 7R is a 3-oxobutyraldehyde reductase (ketone reducing) catalyzing conversion of 3-oxobutyraldehyde to 3 hydroxybutyraldehyde, wherein 7S is a 4-hydroxy-2-butanone reductase catalyzing conversion of4-hydroxy-2-butanone to 1,3-butanediol, wherein 7X is a 3-hydroxybutyryl CoA:ACP transferase catalyzing conversion of 3-hydroxybutyryl-ACP to 3-hydroxybutyryl CoA, wherein 7Y is a 3-hydroxybutyryl-CoA hydrolase, a 3-hydroxybutyryl-CoA transferase or a 3-hydroxybutyryl-CoA synthetase catalyzing conversion of 3-hydroxybutyryl-CoA to 3 hydroxybutyrate, wherein 7Z is a 3-hydroxybutyrate reductase catalyzing conversion of 3 hydroxybutyrate to 3-hydroxybutyraldehyde, and wherein 7AA is a 3-hydroxybutyraldehyde reductase catalyzing conversion of 3-hydroxybutyraldehyde to 1,3-butanediol. 2. The non-naturally occurring microbial organism of claim 1, wherein at least one, two, three, four or five of said exogenous nucleic acids are heterologous nucleic acids. 3. The non-naturally occurring microbial organism of claim 1, wherein each of said exogenous nucleic acids is a heterologous nucleic acid. 220

4. The non-naturally occurring microbial organism of claim 1, wherein said non naturally occurring microbial organism is in a substantially anaerobic culture medium.

5. The non-naturally occurring microbial organism of claim 1, further comprising: (i) a reductive TCA pathway comprising at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein said at least one exogenous nucleic acid is selected from an ATP-citrate lyase, a citrate lyase, a citryl-CoA synthetase, a citryl-CoA lyase, a fumarate reductase, and an alpha-ketoglutarate:ferredoxin oxidoreductase; (ii) a reductive TCA pathway comprising at least one exogenous nucleic acid encoding a reductive TCA pathway enzyme, wherein said at least one exogenous nucleic acid is selected from a pyruvate:ferredoxin oxidoreductase, a phosphoenolpyruvate carboxylase, a phosphoenolpyruvate carboxykinase, a CO dehydrogenase, and an H 2 hydrogenase; or (iii) at least one exogenous nucleic acid encodes an enzyme selected from a CO dehydrogenase, an H 2 hydrogenase, and combinations thereof.

6. The non-naturally occurring microbial organism of claim 5, wherein said microbial organism comprising (i) further comprises an exogenous nucleic acid encoding an enzyme selected from a pyruvate:ferredoxin oxidoreductase, an aconitase, an isocitrate dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, an acetate kinase, a phosphotransacetylase, an acetyl-CoA synthetase, an NAD(P)H:ferredoxin oxidoreductase, ferredoxin, and combinations thereof.

7. The non-naturally occurring microbial organism of claim 5, wherein said microbial organism comprising (ii) further comprises an exogenous nucleic acid encoding an enzyme selected from an aconitase, an isocitrate dehydrogenase, a succinyl-CoA synthetase, a succinyl-CoA transferase, a fumarase, a malate dehydrogenase, and combinations thereof.

8. The non-naturally occurring microbial organism of claim 5, wherein said microbial organism comprising (i) comprises three exogenous nucleic acids encoding an ATP-citrate lyase or a citrate lyase, a fumarate reductase, and an alpha ketoglutarate:ferredoxin oxidoreductase; 221 wherein said microbial organism comprising (ii) comprises four exogenous nucleic acids encoding a pyruvate:ferredoxin oxidoreductase; a phosphoenolpyruvate carboxylase or a phosphoenolpyruvate carboxykinase; a CO dehydrogenase; and an H 2 hydrogenase; or wherein said microbial organism comprising (iii) comprises two exogenous nucleic acids encoding a CO dehydrogenase and an H 2 hydrogenase.

9. The non-naturally occurring microbial organism of any one of claims 1 to 8, wherein said non-naturally occurring microorganism is a species of bacteria, yeast, or fungus.

10. The non-naturally occurring microbial organism of claim 9, wherein said bacteria is selected from the group consisting of Escherichia coli, Klebsiella oxytoca, Anaerobiospirillum succiniciproducens, Actinobacillus succinogenes, Mannheimia succiniciproducens, Rhizobium etli, Bacillus subtilis, Corynebacterium glutamicum, Cupriavidus necator, Gluconobacter oxydans, Zymomonas mobilis, Lactococcus lactis, Lactobacillus plantarum, Streptomyces coelicolor, Clostridium acetobutylicum, Pseudomonas fluorescens, and Pseudomonoas putida.

11. The non-naturally occurring microbial organism of claim 9, wherein said yeast or fungus is selected from the group consisting of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Kluyveromyces marxianus, Aspergillus terreus, Aspergillus niger, Pichia pastoris, Rhizopus arrhizus, Rhiobus oryzae, Yarrowia lipolytica, and Candida albicans.

12. A method for producing 1,3-butanediol, comprising culturing the non-naturally occurring microbial organism of any one of claims 1-11 under conditions and for a sufficient period of time to produce 1,3-butanediol.

13. The method of claim 12, wherein said method further comprises isolating said 1,3-butanediol.

14. The method of claim 13, wherein said isolation is performed by extraction, continuous liquid-liquid extraction, pervaporation, membrane filtration, membrane separation, reverse osmosis, electrodialysis, distillation, crystallization, centrifugation, extractive filtration, ion exchange chromatography, size exclusion chromatography, adsorption chromatography, and ultrafiltration. 222

15. The method of claim 14, wherein said isolation is performed by distillation.

16. A microbial organism according to claim 1, substantially as herein described or exemplified.

17. A method according to claim 12, substantially as herein described or exemplified.