AU2012244091A1 - Multivalent canine vaccines against leptospira bratislava and other pathogens - Google Patents
Multivalent canine vaccines against leptospira bratislava and other pathogens Download PDFInfo
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- AU2012244091A1 AU2012244091A1 AU2012244091A AU2012244091A AU2012244091A1 AU 2012244091 A1 AU2012244091 A1 AU 2012244091A1 AU 2012244091 A AU2012244091 A AU 2012244091A AU 2012244091 A AU2012244091 A AU 2012244091A AU 2012244091 A1 AU2012244091 A1 AU 2012244091A1
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- leptospira
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Abstract
Abstract The present application relates to combination vaccines for immunizing dogs against canine pathogens such as Bordatella bronchiseptica and Leptospira bratislava. The combination vaccine can comprise a Leptospira cell preparation of Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae, Leptospira bratislava, Leptospira pomona and further comprising attenuated strains of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, canine parvovirus (CPV), other pathogens and various carriers and adjuvants. Figure 1: Summary of Geometic Mean of P68 EUSA Endpoint Titers in Unvaccinated and p68 Bordetefla (16 jzgldosa) Vaccinated Dogs Aerosol Challenge with Bordetella Bronchiseptica BOWOG=C SIUU - DOGS SUMMA~RY OF MU EM INIS OMEMN MEWt BY Dftf OF sIUD 0 10 20 30 40506 day af A*ud 000.0 n (3 -6 SM Sdhe6 8 0-0-E)aTM SI9 =.-o-. i imsso 1%m pa Lowg I
Description
AUSTRALIA Patents Act 1990 ORIGINAL COMPLETE SPECIFICATION STANDARD PATENT Invention title: Multivalent canine vaccines against leptospira bratislava and other pathogens This application is a divisional of Australian Patent Application No. 2005290921 which in turn is a divisional Australian Patent Application No. 2004208556 which in turn is the Australian national phase entry of PCT/1B2004/000146 which claims priority to US provisional patent application No. 60/443,418 filed 29 January 2003. Each of these applications are herein incorporated by reference in their entireties. The following statement is a full description of this invention, including the best method of performing it known to us: la MULTIVALENT CANINE VACCINES AGAINST LEPTOSPIRA BRATISLAVA AND OTHER PATHOGENS FIELD OF THE INVENTION This invention relates to vaccines containing a Bordetella bronchiseptica p68 antigen and the use thereof for protecting dogs against infectious tracheobronchitis ("kennel cough") 10 caused by Bordetella bronchiseptica. This invention also relates to combination vaccines containing a Bordetella bronchiseptica p68 antigen and one or more antigens of another canine pathogen such as canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, canine coronavirus (CCV), canine parvovirus (CPV), Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira 15 icterohaemorrhagiae or Leptospira pomona. Methods for protecting dogs against diseases caused by canine pathogens using combination vaccines are also provided. This invention relates to vaccines containing Leptospira bratislava and the use thereof for protecting dogs against infections caused by Leptospira bratislava. This invention also relates to combination vaccines containing Leptospira bratislava and one or more antigens of another canine 20 pathogen such as canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, canine coronavirus (CCV), canine parvovirus (CPV), Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae or Leptospira pomona. This invention further relates to combination vaccines of said antigens without Leptospira bratislava. Methods for protecting dogs against diseases caused by canine pathogens using 25 the combination vaccines are also provided. BACKGROUND OF THE INVENTION The present commercially available canine Bordetella bronchiseptica vaccine product is composed of an inactivated, nonadjuvanted Bordetella bronchiseptica whole cell bacterin. 30 Such whole cell bacterin can lead to cell protein related post-vaccination reactions. The p68 protein of B. bronchiseptica is antigenically similar to the Outer Membrane Protein (OMP) of B. pertussis and the OMP of B. parapertussis (Shahin et al., "Characterization of the Protective Capacity and Immunogenicity of the 69-kD Outer Membrane Protein of Bordetella pertussis", J. Exp. Med.. 171: 63-73, 1990). A protective role of this OMP has been demonstrated for 35 mice (Shahin et al., supra; Novotny et al., "Biologic and Protective Properties of the 69-kD Outer Membrane Protein of Bordetella pertussis: A Novel Formulation for a Acellular Pertussis Vaccine", J. Infect. Dis. 164:114-22, 1991), humans (He et al., "Protective Role of Immunoglobulin G Antibodies to Filamentous Hemagglutinin and Pertactin of Bordetella pertussis in Bordetela parapertussis Infection", Eur. J Clin Microbiol Infect Dis. 10:793-798, 40 1996) and swine (Kobisch et al., " Identification of a 68-Kilodalton Outer Membrane Protein as the Major Protective Antigen of Bordetella bronchiseptica by Using Specific-Pathogen-Free Piglets", Infect. Immun. 58(2):352-357, 1990). Prior to the present invention, there had been no showing that a Bordetella bronchiseptica p68 antigen can be a safe and effective vaccine in dogs. Therefore, there is a 2 5 need to develop a Bordetella bronchiseptica vaccine containing a p68 antigen that is suitable for canine use. It would be even more advantageous if such a Bordetella bronchiseptica p68 vaccine is safe for administration to puppies and provides a long-term protection. CD is a universal, high-mortality viral disease with variable manifestations. Approximately 50% of nonvaccinated, nonimmune dogs infected with CD virus develop clinical 10 signs, and approximately 90% of those dogs die. Infectious canine hepatitis or ICH, caused by canine adenovirus type 1 (CAV-1), is a universal, sometimes fatal, viral disease of dogs characterized by hepatic and generalized endothelial lesions. CAV-2 causes respiratory disease, which, in severe cases, may include pneumonia and bronchopneumonia. 15 CPI is a common viral upper respiratory disease. Uncomplicated CPI may be mild or subclinical, with signs becoming more severe if concurrent infection with other respiratory pathogens exists. CPV infection results in enteric disease characterized by sudden onset of vomiting and diarrhea, often hemorrhagic. Leukopenia commonly accompanies clinical signs. 20 Susceptible dogs of any age can be affected, but mortality is greatest in puppies. In puppies 4-12 weeks of age CPV may occasionally cause myocarditis that can result in acute heart failure after a brief and inconspicuous illness. Following infection many dogs are refractory to the disease for a year or more. Similarly, seropositive bitches may transfer to their puppies CPV antibodies which can interfere with active immunization of the puppies through 16 weeks 25 of age. CCV also causes enteric disease in susceptible dogs of all ages worldwide. Highly contagious, the virus is transmitted primarily through direct contact with infectious feces, and may cause clinical enteritis within 1-4 days after exposure. Severity of disease may be exacerbated by concurrent infection with other agents. Primary signs of CCV infection include 30 anorexia, vomiting, and diarrhea. Frequency of vomiting usually diminishes within a day or 2 after onset of diarrhea, but diarrhea may linger through the course of infection, and stools occasionally may contain streaks of blood. With CCV infection most dogs remain afebrile and leukopenia is not observed in uncomplicated cases. Leptospirosis occurs In dogs of all ages, with a wide range of clinical signs and 35 chronic nephritis generally following acute infection. Some combination vaccines have been developed, including those sold under the Vanguard@ tradename. However, prior to the present invention, there have been no effective combination vaccines that protect dogs against Bordetella bronchiseptica and one or more of other canine pathogens such as CD virus, CAV-2, CPI virus, CPV, CCV, and a Leptospira 40 species such as L. bratislava, L. canicola, L. grippotyphosa, L. icterohaemorrhagiae and L. pomona. There also have been no effective combination vaccines comprising L. Bratislava against these other canine pathogens but without Bordetella bronchiseptica. A problem in developing combination vaccines involves efficacy interference, namely a failure of one or more antigens in a combination composition to maintain or achieve efficacy because of the 3 5 presence of the other antigens in the composition. This is believed to be a result of interference with an antigen in the composition administered to a host, e.g., a dog, in the immunological, antigenic, antibody or protective response such antigen induced in the host because of the other antigens present in the composition. However, for other hosts, such as cats, combination vaccines are known. It is believed that efficacy interference in dogs is due 10 to some peculiarity of the canine biological system, or due to the reaction of the antigens with the canine biological system. There is a need, therefore, to develop a combination vaccine suitable for administration to dogs against Bordetella bronchiseptica and one or more other canine pathogens, which does not exhibit efficacy interference in canines. There is also a need to 15 develop such combination vaccines without Bordetella bronchiseptica. It would be even more advantageous if such combination vaccines are safe for administration to puppies and provide long-term protection. SUMMARY OF THE INVENTION 20 The present invention provides vaccines and methods for protecting dogs against diseases caused by canine pathogens. In one embodiment, the present invention provides p68 vaccines suitable for administration to dogs and capable of protecting dogs against disease caused by Bordetella bronchiseptica. Such vaccines of the present invention include a Bordetella bronchiseptica 25 p68 antigen and a veterinary-acceptable carrier such as an adjuvant. In another embodiment, the present invention provides methods of protecting dogs against disease caused by Bordetella bronchiseptica by administering to a dog a vaccine which includes a Bordetella bronchiseptica p68 antigen and.a veterinary-acceptable carrier such as an adjuvant. 30 In another embodiment, the present invention provides Leptospira bratislava vaccines suitable for administration to dogs and capable of protecting dogs against disease caused by Leptospira bratislava. Such vaccines of the present invention include a cell preparation of Leptospira bratislava and a veterinary-acceptable carrier such as an adjuvant. In another embodiment, the present invention provides methods of protecting dogs 35 against disease caused by Leptospira bratislava by administering to a dog a vaccine which includes a cell preparation of Leptospira bratislava and a veterinary-acceptable carrier such as an adjuvant. In still another embodiment, the present invention provides combination vaccines suitable for administration to dogs. The combination vaccines of the present invention include 40 a Bordetella bronchiseptica p68 antigen in combination with at least one other antigen from other canine pathogens, capable of inducing a protective immune response in dogs against disease caused by such other pathogen(s). Such other pathogens can be selected from canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, canine parvovirus (CPV), canine coronavirus (CCV), canine herpesvirus, rabies virus, 4 5 Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae, Leptospira pomona, Leptospira hardjobovis, Porphyromonas spp., Bacteriodes spp., Leishmania spp., Borrelia spp., Ehrlichia spp., Mycoplasma spp. and Microsporum canis. A preferred combination of the present invention includes two or more antigens from 10 canine pathogens, capable of inducing a protective immune response in dogs against disease caused by such pathogen(s). Such pathogens can be selected from canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, canine parvovirus (CPV), canine coronavirus (CCV), canine herpesvirus, rabies virus, Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae, Leptospira 15 pomona, Leptospira hardjobovis, Porphyromonas spp., Bacteriodes spp., Leishmania spp., Borrelia spp., Ehrlichia spp., Mycoplasma spp. and Microsporum canis. A preferred combination vaccine of the present invention includes attenuated strains of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus and canine parvovirus (CPV); an inactivated preparation of a strain of canine coronavirus 20 (CCV); and a Bordetella bronchiseptica p68 antigen. A preferred combination vaccine of the present invention includes attenuated strains of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, and canine parvovirus (CPV); and an inactivated preparation of a strain of canine coronavirus (CCV). 25 Another preferred combination vaccine of the present invention includes attenuated strains of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus and canine parvovirus (CPV); an Inactivated preparation of a strain of canine coronavirus (CCV); a Bordetella bronchiseptica p68 protein, and an inactivated cell preparation of five Leptospira serovars (Leptospira bratisiava, Leptospira canicola, Leptospira 30 grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona). Another preferred combination vaccine of the present invention includes attenuated strains of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, and canine parvovirus (CPV); and an inactivated preparation of a strain of canine coronavirus (CCV); and a cell preparation of five Leptospira serovars 35 (Leptospira bratislava, Leptospira canicola, Leptospira gippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona). Another preferred combination vaccine of the present invention includes attenuated strains of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, and canine parvovirus (CPV); an inactivated preparation of a strain 40 of canine coronavirus (CCV); and an inactivated cell preparation of four Leptospira serovars (Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona).
5 5 Still another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain; and a Bordetella bronchiseptica p68 antigen. Still another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain. 10 Another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain; a Bordetella bronchiseptica p68 antigen; and an inactivated cell preparation of Leptospira canicola and Leptospira icterohaemorrhagiae. Another preferred combination vaccine of the present invention includes attenuated 15 strains of CD virus, CAV-2, CPI virus, a CPV strain; and an inactivated cell preparation of Leptospira canicola and Leptospira icterohaemorrhagiae. Still another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain, a Bordetella bronchiseptica p68 antigen and an inactivated cell preparation of five Leptospira serovars (Leptospira 20 bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona). Still another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain, and an inactivated cell preparation of five Leptospira serovars (Leptospira bratislava, Leptospira canicola, Leptospira 25 grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona). Still another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain, and an inactivated cell preparation of four-Leptospira serovars (Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona). 30 Another preferred combination vaccine includes a Bordetella bronchiseptica p68 antigen and an attenuated CPI virus. Still another preferred combination vaccine includes a Bordetella bronchiseptica p68 antigen, an attenuated CPI virus and an inactivated cell preparation of Leptospira canicola and Leptospira icterohaemorrhagiae. 35 The present invention also provides methods of protecting dogs against disease caused by a canine pathogen by administering to a dog a combination vaccine of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS 40 Figure 1. Summary of the geometric mean of p68 ELISA endpoint titers in unvaccinated and Bordetella p68 (15 gg/dose) vaccinated dogs-aerosol challenge with Bordetella bronchiseptica. Figure 2. Summary of Serum Amyloid A titers in dogs following aerosol challenge with Bordetella bronchiseptica.
6 5 Figure 3. Summary of the geometric mean of p68 ELISA endpoint titers in unvaccinated and Bordetella p68 vaccinated dogs following vaccination and aerosol challenge with Bordetella bronchiseptica. Figure 4. Summary of Serum Amyloid A titers in dogs following aerosol challenge with Bordetella bronchiseptica. 10 Figure 5. Western blot showing reactivity of p68 monoclonal antibody Bord 2-7 to p68 whole cell lysate. DETAILED DESCRIPTION OF THE INVENTION In one embodiment, the present invention provides monovalent vaccines suitable for administration to dogs which are capable of protecting dogs against disease caused by 15 Bordetella bronchiseptica. The monovalent vaccines of the present invention include a recombinantly produced Bordetella bronchiseptica p68 antigen and a veterinary-acceptable carrier such as an adjuvant. In another embodiment, the present invention provides methods of protecting dogs against disease caused by Bordetella bronchiseptica by administering to a dog a monovalent 20 vaccine which includes a recombinantly produced Bordetella bronchiseptica p68 antigen and a veterinary-acceptable carrier such as an adjuvant. In still another embodiment, the present invention provides combination vaccines suitable for administration to dogs. The combination vaccines of the present invention include a recombinantly produced Bordetella bronchiseptica p68 antigen in combination with at least 25 one other antigen capable of inducing a protective immune response in dogs against disease caused by such other antigen. Another embodiment of the present invention includes two or more antigens from canine pathogens, capable of inducing a protective immune response in dogs against disease caused by such pathogen(s). A preferred combination vaccine of the present invention includes attenuated strains 30 of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus and canine parvovirus (CPV); an inactivated preparation of a strain of canine coronavirus (CCV); and a preparation of four Leptospira serovars (Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae, and Leptospira pomona). Another preferred combination vaccine of the present invention includes attenuated 35 strains of canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus and canine parvovirus (CPV); an inactivated preparation of a strain of canine coronavirus (CCV); and a preparation of five Leptospira serovars (Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona). 40 Still another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain; and a preparation of four Leptospira serovars (Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona)..
7 5 Another preferred combination vaccine of ihe present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain, a CCV strain; and a preparation of Leptospira canicola and Leptospira icterohaemorrhagiae. Still another preferred combination vaccine of the present invention includes attenuated strains of CD virus, CAV-2, CPI virus, a CPV strain and a preparation of five 10 Leptospira serovars (Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona). The present invention also provides methods of protecting dogs against disease caused by a canine pathogen by administering to a dog a combination vaccine of the present invention. 15 For clarity of disclosure, and not by way of limitation, the detailed description of the invention is divided into the following subsections which describe or illustrate certain features, embodiments or applications of the invention. Definitions and Abbreviations 20 The term "protecting a dog against a disease caused by a canine pathogen" as used herein means reducing or eliminating the risk of infection by the pathogen, ameliorating or alleviating the symptoms of an infection, or accelerating the recovery from an infection. Protection is achieved if there is a reduction in viral or bacterial load, a reduction in viral or bacterial shedding, a decrease in incidence or duration of infections, reduced acute phase 25 serum protein levels, reduced rectal temperatures, and/or increase in food uptake and/or growth, for example. The term "p68 antigen" refers to a protein with a molecular weight of 68 kDa as determined by SDS polyacrylamide gel electrophoresis, is recognized by the p68-specific monoclonal antibody Bord 2-7 (ATCC#), and has an amino acid sequence as set forth in SEQ 30 ID NO: 1 or an amino acid sequence that is substantially identical to SEQ ID NO: 1. By "substantially identical" is meant a degree of sequence identity of at least about 90%, preferably at least about 95%, or more preferably, at least about 98%. The term "monovalent vaccine" as used herein refers to a vaccine having one principal antigenic component. For example, a p68 monovalent vaccine includes a Bordetella 35 bronchiseptica p68 antigen as the principal antigenic component of the vaccine and is capable of protecting the animal to which the vaccine is administered against diseases caused by Bordetella bronchiseptica. Another example of a monovalent vaccine includes a cell preparation of Leptospira bratislava as the principal antigenic component of the vaccine and is capable of protecting the animal to which the vaccine is administered against diseases caused 40 by Leptospira bratislava. The term "combination vaccine" is meant a bivalent or multivalent combination of antigens which are capable of inducing a protective immune response in dogs. The protective effects of a combination vaccine against a pathogen or pathogens are normally achieved by inducing in the animal subject an immune response, either a cell-mediated or a humoral 45 immune response or a combination of both.
8 5 By "immunogenic" is meant the capacity of a composition to provoke an immune response in dogs against a particular pathogen. The immune response can be a cellular immune response mediated primarily by cytotoxic T-cells and cytokine-producing T-cells, or a humoral immune response mediated primarily by helper T-cells, which in turn activates B-cells leading to antibody production. 10 The term "therapeutically effective amount" or "effective amount" refers to an amount of a monovalent or combination vaccine sufficient to elicit a protective immune response in the dog to which it is administered. The immune response may comprise, without limitation, induction of cellular and/or humoral immunity. The amount of a vaccine that is therapeutically effective may vary depending on the particular antigen used in the vaccine, the age and 15 condition of the dog, and/or the degree of infection, and can be determined by a veterinary physician. p68 Vaccines The present invention has demonstrated for the first time that a vaccine composition containing a Bordetella bronchiseptica p68 antigen effectively protected dogs against disease 20 caused by Bordetella bronchiseptica. The vaccine composition of the present invention does not cause significant post-vaccination reactions, is safe for administration to puppies, and induces protective immunity in dogs that lasts for an extended period of time. Accordingly, one embodiment of the present invention is directed to a vaccine composition containing a Bordetella bronchiseptica p68 antigen (or "a p68 vaccine"), that is 25 suitable for administration to dogs and is capable of protecting dogs against disease caused by Bordetella bronchiseptica, e.g., infectious tracheobronchitis ("kennel cough"). For the purpose of the present invention, the term "p68 antigen" refers to a protein (see Figure 5) with a molecular weight of 68 kDa as determined by SDS polyacrylamide gel electrophoresis, is recognized by the p68-specific monoclonal antibody Bord 2-7 (ATCC# ), 30 and has an amino acid sequence as set forth in SEQ ID NO: 1 or an amino acid sequence that is substantially identical to SEQ ID NO: 1. By "substantially identical" is meant a degree of sequence identity of at least about 90%, preferably at least about 95%, or more preferably, at least about 98%. An example of a p68 antigen having an amino acid sequence substantially identical to SEQ ID NO: 1 is the p68 antigen described in WO 92/17587, which is set forth in 35 SEQ ID NO: 3. The p68 specific monoclonal antibody of the present invention recognizes native p68 proteins, recombinant p68 proteins and p68 proteins on the surface of bacteria, for example. In accordance with the present invention, p68 antigens suitable for use in the present invention include both native p68 proteins (i.e., naturally occurring p68 proteins purified from 40 Bordetella bronchiseptica) and recombinantly produced p68 proteins. Purification of native p68 from Bordetella bronchiseptica is described, e.g., in Montaraz et al., Infection and Immunity 47: 744-751 (1985), and is also illustrated in the examples provided hereinbelow. Recombinant production of p68 can be achieved using any one of the molecular cloning and recombinant expression techniques known to those skilled in 9 5 the art. For example, a nucleic acid molecule encoding p68 can be introduced into an appropriate host cell, such as a bacterium, a yeast cell (e.g., a Pichia cell), an insect cell or a mammalian cell (e.g., CHO cell). The p68-encoding nucleic acid molecule can be placed in an operable linkage to a promoter capable of effecting the expression of the p68 antigen in the host cell. p68, which is expressed by the host cell, can be readily purified using routine protein 10 purification techniques. In a preferred embodiment of the present invention, the nucleotide sequence as set forth in.SEQ ID NO: 2 coding for the p68 antigen which has the amino acid sequence of SEQ ID NO: 1, is cloned in an expression vector and placed in an operable linkage to a temperature sensitive promoter. The expression vector is introduced into Escherichia coliand 15 the p68 antigen is expressed upon heat induction. The cells are lysed and the inclusion bodies where the p68 antigen accumulates are separated by centrifugation. The recombinant p68 in the inclusion bodies is solubilized using SDS or other solubilization agents known in the art such as urea, guanidine hydrochloride, sodium cholate, taurocholate, and sodium deoxycholate. In accordance with the present invention, a purified native or recombinant p68 20 protein is combined with a veterinary-acceptable carrier to form a p68 vaccine composition. The term "a veterinary-acceptable carrier" includes any and all solvents, dispersion media, coatings, adjuvants, stabilizing agents, diluents, preservatives, antibacterial and antifungal agents, isotonic agents, adsorption delaying agents, and the like. Diluents can include water, saline, dextrose, ethanol, glycerol, and the like. Isotonic agents can include 25 sodium chloride, dextrose, mannitol, sorbitol, and lactose, among others. Stabilizers include albumin, among others. Adjuvants suitable for use in accordance with the present invention include, but are not limited to several adjuvant classes such as; mineral salts, e.g., Alum, aluminum hydroxide, aluminum phosphate and calcium phosphate; surface-active agents and microparticles, e.g., 30 nonionic block polymer surfactants (e.g., cholesterol), virosomes, saponins (e.g., Quil A, QS 21 and GPI-01 00), proteosomes, immune stimulating complexes, cochleates, quarterinary amines (dimethyl diocatadecyl ammonium bromide (DDA)), avridine, vitamin A, vitamin E; bacterial products such as the RIBI adjuvant system (Ribi Inc.), cell wall skeleton of Mycobacterum phlei (Detox@), muramyl dipeptides (MDP) and tripeptides (MTP), 35 monophosphoryl lipid A, Bacillus Calmete-Guerin, heat labile E. coli enterotoxins, cholera toxin, trehalose dimycolate, CpG oligodeoxnucleotides; cytokines and hormones, e.g., interleukins (IL-1, IL-2, IL-6, IL-1 2, IL-1 5, IL-1 8), granulocyte-macrophage colony stimulating factor, dehydroepiandrosterone, 1,25-dihydroxy vitamin D 3 ; polyanions, e.g., dextran; polyacrylics (e.g., polymethylmethacrylate, Carbopol 934P); carriers e.g., tetanus toxid, 40 diptheria toxoid, cholera toxin B subnuit, mutant heat labile enterotoxin of enterotoxigenic E. coli (rmLT), heat shock proteins; oil-in-water emulsions e.g.,AMPHIGEN* (Hydronics, USA); and water-in-oil emulsions such as, e.g., Freund's complete and incomplete adjuvants. Preferred adjuvants for use in the vaccines of the present invention include Quil A and cholesterol.
10 5 The p68 antigen and the veterinary-acceptable carrier can be combined in any convenient and practical manner to form a vaccine composition, e.g., by admixture, solution, suspension, emulsification, encapsulation, absorption and the like, and can be made in formulations such as tablets, capsules, powder, syrup, suspensions that are suitable for injections, implantations, inhalations, ingestions or the like. Preferably, the vaccine is 10 formulated such that it can be administered to dogs by injection in a dose of about 0.1 to 5 ml, or preferably about 0.5 to 2.5 ml, or even more preferably, in a dose of about 1 ml. When appropriate, the pharmaceutical compositions of the present invention should be made sterile by well-known procedures. The amount of p68 in the vaccines should be immunizing-effective and is generally in 15 the range of 0.5 - 1000 pg per dose. Preferably, the amount of p68 is in the range of 1-260 pg per dose. More preferably, the amount of p68 is in the range of 10-100 pg per dose. Even more preferably, the amount of p68 is about 15 to 25 pg per dose. The amount of adjuvants suitable for use in the vaccines depends upon the nature of the adjuvant used. For example, when Quil A and cholesterol are used as adjuvant, Quil A is 20 generally in an amount of about 1-1000 pg per dose, preferably 30-100 pg per dose, and more preferably, about 50-75 pig per dose; and cholesterol is generally in an amount of about 1 1000 pg peridose, preferably about 30-100 lag per dose, and more preferably, about 50-75 g per dose. In another embodiment, the present invention provides methods of protecting dogs 25 against disease caused by Bordetella bronchiseptica by administering to a dog a p68 vaccine composition, as described hereinabove. In accordance with the present invention, the p68 vaccine composition provides dogs with a long term immunity for at least about 4 months, preferably for at least about 6 months, or even more preferably, for about one year, In accordance with the present invention, a p68 vaccine can be administered to a dog 30 by any known routes, including the oral, intranasal, mucosal, topical, transdermal, and parenteral (e.g., intravenous, intraperitoneal, intradermal, subcutaneous or intramuscular). Administration can also be achieved using needle-free delivery devices. Administration can be achieved using a combination of routes, e.g., first administration using a parental route and subsequent administration using a mucosal route. 35 Preferred routes of administration include subcutaneous and intramuscular administrations. The p68 vaccine composition of the present invention can be administered to dogs of at least 6 weeks old, preferably at least 7 weeks old, and more preferably, at least 8 or 9 weeks old. Dogs can be vaccinated with one dose orwith more than one dose of a p68 40 vaccine. Preferably, two doses of a p68 vaccine are administered to dogs with an interval of about 2-4 weeks, preferably about 3 weeks, between the two administrations. If dogs are vaccinated before the age of 4 months, it is recommended that they be revaccinated with a single dose upon reaching 4 months of age, because matemal antibodies may interfere with development of an adequate immune response in puppies less than 4 months old. Dogs can 11 5 also be revaccinated annually with a single dose. Where B. bronchiseptica exposure is likely, such as breeding, boarding, and showing situations, an additional booster may be given within 1 year, or preferably 6 months, of the occurrence of these events. Combination Vaccines 10 In another embodiment, the present invention provides combination vaccines and methods for protecting dogs against Bordetella bronchiseptica and/or one or more other canine pathogens by administering such combination vaccines. The combination vaccine compositions of the present invention do not exhibit efficacy interference and are safe for administration to puppies. 15 The combination vaccines of the present invention include a Bordetella bronchiseptica p68 antigen, which can be made as described hereinabove, in combination with at least one antigen from other canine pathogens capable of inducing a protective immune response in dogs against disease caused by such other pathogens. Such combination vaccines also include combinations of two or more such other canine pathogens without the p68 antigen. 20 Such other pathogens include, but are not limited to, canine distemper (CD) virus, canine adenovirus type 2 (CAV-2), canine parainfluenza (CPI) virus, canine parvovirus (CPV), canine coronavirus (CCV), canine herpesvirus, and rabies virus. Antigens from these pathogens for use in the vaccine compositions of the present invention can be in the form of a modified live viral preparation or an inactivated viral preparation. Methods of attenuating 25 virulent strains of these viruses and methods of making an inactivated viral preparation are known in the art and are described in, e.g., U.S. Patents 4,567,042 and 4,567,043. Other pathogens also include Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae, Leptospira pomona, Leptospira hardjobovis, Porphyromonas spp., Bacteriodes spp., Leishmania spp., Borrelia spp., Ehrlichia spp., 30 Mycoplasma ssp. and Microsporum canis. Antigens from these pathogens for use in the vaccine compositions of the present invention can be in the form of an inactivated whole or partial cell preparation, using methods well-known in the art. For example, methods of making an inactivated whole or partial Leptospira cell preparation are known in the art and are described in, e.g., Yan, K-T, "Aspects of Immunity to Leptospira borgpeterseni serovar 35 hardjd', PhD Thesis, Appendix 1, 1996. Faculty of Agriculture and Food Science, The Queen's University of Belfast; Mackintosh et al., "The use of a hardjo-pomona vaccine to prevent leptospiruria in cattle exposed to natural challenge with Leptospia interrogans serovar hardjo", New Zealand Vet. J. 28:174-177, 1980;Bolin et. al., "Effect of vaccination with a pentavalent leptopsiral vaccine on Leptospira interrogans serovar hardjo type hardjo-boivs infection of 40 pregnant cattle", Am. J. Vet. Res. 50:161-165, 1989. In accordance with the present invention, the combination vaccines generally include a veterinary-acceptable carrier. As described hereinabove, a veterinary-acceptable carrier includes any and all solvents, dispersion media, coatings, adjuvants, stabilizing agents, diluents, preservatives, antibacterial and antifungal agents, isotonic agents, adsorption 12 5 delaying agents, and the like. Diluents can include water, saline, dextrose, ethanol, glycerol, and the like. Isotonic agents can include sodium chloride, dextrose, mannitol, sorbitol, and lactose, among others. Stabilizers include albumin, among others. Adjuvants suitable for use in accordance with the present invention include, but are not limited to several adjuvant classes such as; mineral salts, e.g., Alum, aluminum hydroxide, 10 aluminum phosphate and calcium phosphate; surface-active agents and microparticles, e.g., nonionic block polymer surfactants (e.g., cholesterol), virosomes, saponins (e.g., Quil A, QS 21 and GPI-0100), proteosomes, immune stimulating complexes, cochleates, quarterinary amines (dimethyl diocatadecyl ammonium bromide (DDA)), avridine, vitamin A, vitamin E; bacterial products such as the RIBI adjuvant system (Ribi Inc.), cell wall skeleton of 15 Mycobacterum phlei (Detox@), muramyl dipeptides (MDP) and tripeptides (MTP), monophosphoryl lipid A, Bacillus Calmete-Guerin, heat labile E. coli enterotoxins, cholera toxin, trehalose dimycolate, CpG oligodeoxnucleotides; cytokines and hormones, e.g., interleukins (IL-1, IL-2, IL-6, IL-12, IL-15, IL-18), granulocyte-macrophage colony stimulating factor, dehydroepiandrosterone, 1,25-dihydroxy vitamin D 3 ; polyanions, e.g., dextran; 20 polyacrylics (e.g., polymethylmethacrylate, Carbopol 934P); carriers e.g., tetanus toxid, diptheria toxoid, cholera toxin B subnuit, mutant heat labile enterotoxin of enterotoxigenic E. coli (rmLT), heat shock proteins; oil-in-water emulsions e.g.,AMPHIGEN* (Hydronics, USA); and water-in-oil emulsions such as, e.g., Freund's complete and incomplete adjuvants. Preferred adjuvants for use in the combination vaccines in accordance with the 25 present invention include 1) Quil A plus cholesterol; and 2) aluminum hydroxide. The amount of adjuvants suitable for use in the vaccines depends upon the nature of the adjuvant used. For example, when Quil A and cholesterol are used as adjuvant, Quil A is generally in an amount of about 1-1000 gg per dose, preferably 30-100 jig per dose, and more preferably, about 50-75 gg per dose; and cholesterol is generally in an amount of about 1-1000 ig per 30 dose, preferably about 30-100 gg per dose, and more preferably, about 50-75 jg per dose. When aluminum hydroxide is used as adjuvant, it is generally in an amount of about 0.5-20%, preferably about 0.5-10%, and more preferably about 1-2%. The p68 antigen, one or more antigens from other pathogens, and/or the veterinary acceptable carrier can be combined in any convenient and practical manner to form a 35 combination vaccine composition, e.g., by admixture, solution, suspension, emulsification, encapsulation, absorption and the like, and can be made in formulations such as tablets, capsules, powder, syrup, suspensions that are suitable for injections, implantations, inhalations, ingestions or the like. Preferably, the vaccine is formulated such that it can be administered to dogs by injection in a dose of about 0.1 to 5 ml, or preferably about 0.5 to 2.5 40 ml, or even more preferably, in a dose of about 1 ml. Combination vaccines may prepared by rehydrating a freeze-dried preparation of the attenuated viral strains (or a preparation made by other methods such as spray drying or desiccation) and viral preparation with a liquid preparation, which liquid preparation is composed of the Leptospiral antigens, dissolved in sterile saline solution and adjuvanted with 13 5 Quil A and cholesterol. Such combination vaccine may also be prepared by rehydrating a freeze-dried preparation of the attenuated viral strains and Leptospira viral preparation (or a preparation made by other methods such as spray drying or desiccation) with a sterile solution, or rehydrating said freeze-dried preparation with CCV plus diluent. In accordance with the present invention, combination vaccines can be administered 10 to a dog of at least 6 weeks old, preferably at least 7 weeks old, and more preferably, at least 8 or 9 weeks old. The combination vaccines can be administered in 2 to 4 doses, preferably in 2 to 3 doses. The doses can be administered with 2 to 6 weeks between each dose, preferably with 2 to 4 weeks between each dose. The administration can be done by any known routes, including the oral, intranasal, 15 mucosal topical, transdermal, and parenteral (e.g., intravenous, intraperitoneal, intradermal, subcutaneous or intramuscular). Administration can also be achieved using needle-free delivery devices. Administration can also be achieved using a combination of routes, e.g., first administration using a parental route and subsequent administration using a mucosal route. Preferred routes of administration include subcutaneous and intramuscular administrations. 20 Preferred Combination Vaccines and Vaccination Methods A preferred combination vaccine of the present invention includes an attenuated strain of CD virus, an attenuated strain of CAV-2, an attenuated strain of CPI virus, an attenuated strain of CPV, an inactivated preparation of a strain of CCV, and a Bordetella bronchiseptica 25 p68antigen. An especially preferred combination vaccine includes the attenuated CD virus strain designated as the "Snyder Hill" strain (National Veterinary Service Laboratory, Ames, IA), the attenuated CAV-2 strain designated as the "Manhattan" strain (National Veterinary Service Laboratory, Ames, IA), the attenuated CPI virus strain having the designation of "NL-CPI-5" 30 (National Veterinary Service Laboratory, Ames, IA ), the attenuated CPV strain having the designation of "NL-35-D" (National Veterinary Service Laboratory, Ames, IA), an inactivated preparation of the CCV strain having the designation of "NL-1 8" (National Veterinary Service Laboratory, Ames, IA), and the recombinant Bordetella bronchiseptica p68 antigen having the sequence of SEQ ID NO: 1. Such combination vaccine, also referred to herein as "the 35 p68/5CV combination vaccine", is preferably prepared by rehydrating a freeze-dried preparation of the attenuated viral strains and viral preparation with a liquid preparation, which liquid preparation is composed of the p68 antigen dissolved in sterile saline solution and adjuvanted with Quil A and cholesterol. This combination without the p68 antigen is referred to herein as the 5CV combination. Such combination vaccine is preferably prepared by 40 rehydrating a freeze-dried preparation of the attenuated viral strains and viral preparation with a liquid preparation, which liquid preparation is composed of sterile saline solution and adjuvanted with Quil A and cholesterol. Another especially preferred combination vaccine includes the antigenic components of the p68/5CV combination vaccine as well as inactivated whole cell preparations of five 14 5 Leptospira species: Leptospira bratislava (e.g., a Leptospira bratislava strain which can be obtained from National Veterinary Service Laboratory, Ames, IA), Leptospira canicola (e.g., strain C-5, National Veterinary Service Laboratory, Ames, IA), Leptospira grippotyphosa (e.g., strain MAL 1540, National Veterinary Service Laboratory, Ames, IA.), Leptospira icterohaemorrhagiae (e.g., strain NADL 11403, National Veterinary Service Laboratory, Ames, 10 IA ) and Leptospira pomona (e.g., strain T262, National Veterinary Service Laboratory, Ames, IA). Such combination vaccine, also referred to herein as "the p68/5CV-Leptospira combination vaccine", is preferably prepared by rehydrating a freeze-dried preparation of the attenuated viral strains (or a preparation made by other methods such as spray drying or desiccation) and viral preparation with a liquid preparation, which liquid preparation is 15 composed of the p68 antigen and Leptospiral antigens, dissolved in sterile saline solution and adjuvanted with Quil A and cholesterol. This combination without the p68 antigen is referred to herein as the 5CV-5Leptospira combination. This combination without the p68 antigen and without Leptospira bratislava is referred to herein as the 5CV-4Leptospira combination. The 5CV combination without the p68 antigen and with Leptospira canicola and Leptospira 20 icterohaemorrhagiae is referred to herein as the 5CV-2Leptospira combination. Such combination vaccines are preferably prepared by rehydrating a freeze-dried preparation of the attenuated viral strains (or a preparation made by other methods such as spray drying or desiccation) and viral preparation with a liquid preparation, which liquid preparation is composed of the Leptospiralantigens, dissolved in sterile saline solution and adjuvanted with 25 Quil A and cholesterol. Such combination vaccines are also preferably prepared by rehydrating a freeze-dried preparation of the attenuated viral strains and Leptospira viral preparation (or a preparation made by other methods such as spray drying or desiccation) with a sterile solution, or rehydrating said freeze-dried preparation with CCV plus diluent. In accordance with the present invention, the p68/5CV, p68/5CV-Leptospira, 5CV, 30 5CV-5Leptospira, 5CV-4Leptospira, and 5CV-2Leptospira combination vaccines can be administered to healthy dogs 4 weeks of age or older, preferably 6 weeks or older, and preferably in 3 doses, each administered about 3 weeks apart. Dogs can be revaccinated annually with a single dose. Where B. bronchiseptica and/or canine virus exposure is likely, such as breeding, boarding, and showing situations, an additional booster may be given within 35 1 year, or preferably 6 months, of the occurrence of these events. .Still another preferred combination vaccine of the present invention includes an attenuated strain of CD virus, an attenuated strain of CAV-2, an attenuated strain of CPI virus, an attenuated strain of CPV, and a recombinant Bordetella bronchiseptica p68 antigen. An especially preferred combination vaccine includes the attenuated CD virus strain 40 designated as the "Synder Hill" strain (National Veterinary Service Laboratory, Ames, IA), the attenuated CAV-2 strain designated as the "Manhattan" strain (National Veterinary Service Laboratory, Ames, IA), the attenuated CPI virus strain having the designation of "NL-CPI-5" (National Veterinary Service Laboratory, Ames, IA), the attenuated CPV strain designated as "NL-35-D" (National Veterinary Service Laboratory, Ames, IA), and the recombinant Bordetella 15 5 bronchiseptica p68 antigen having the sequence of SEQ ID NO: 1. Such combination vaccine, also referred to herein as "the p68/DA 2 PP combination vaccine", is preferably prepared by rehydrating a freeze-dried preparation of the attenuated viral strains (or a preparation made by other methods such as spray drying or desiccation) with a liquid preparation, which liquid preparation is composed of the p68 antigen dissolved in sterile saline 10 solution and adjuvanted with Quil A and cholesterol. This combination vaccine without the p68 antigen is referred to as the DA 2 PP combination vaccine. Such combination vaccine is preferably prepared by rehydrating a freeze-dried preparation of the attenuated viral strains (or a preparation made by other methods such as spray drying or desiccation) with a liquid preparation, which liquid preparation is composed of sterile saline solution and adjuvanted 15 with Quil A and cholesterol. Another especially preferred combination vaccine includes the antigenic components of the p68/DA 2 PP combination vaccine as well as inactivated whole cell preparations of two Leptospira species: Leptospira canicola (e.g., strain C-51, National Veterinary Service Laboratory, Ames, IA), and Leptospira icterohaemorrhagiae (e.g., strain NADL 11403, 20 National Veterinary Service Laboratory, Ames, IA). Alternatively, a preferred combination vaccine can include the antigenic components of the p68/DA 2 PP combination vaccine as well as inactivated whole cell preparations of five Leptospira species: Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona. These combination vaccines, also referred to herein as "the p68/DA 2
PP
25 Leptospira combination vaccines", are preferably prepared by rehydrating a freeze-dried preparation of the attenuated viral strains (or a preparation made by other methods such as spray drying or desiccation) and viral preparation with a liquid preparation, which liquid preparation is composed of the p68 antigen and Leptospiral antigens, dissolved in sterile saline solution and adjuvanted with Quil A and cholesterol. 30 Alternatively, another preferred combination vaccine includes the antigenic components of the DA 2 PP combination vaccine as well as inactivated whole cell preparations of five Leptospira species: Leptospira bratislava, Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona. Still another preferred combination vaccine includes the antigenic components of the DA 2 PP combination 35 vaccine as well as inactivated whole cell preparations of four Leptospira species: Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae and Leptospira pomona. These combination vaccines, also referred to herein as "the DA 2 PP-Leptospira combination vaccines", are preferably prepared by rehydrating a freeze-dried preparation of the attenuated viral strains (or a preparation made by other methods such as spray drying or desiccation) and 40 viral preparation with a liquid preparation, which liquid preparation is composed of the Leptospiral antigens, dissolved in sterile saline solution and adjuvanted with Quil A and cholesterol. In accordance with the present invention, the p6B/DA 2 PP, p68/DA 2 PP-Leptospira,
DA
2 PP, and DA 2 PP-Leptospira combination vaccines can be administered to healthy dogs 6 16 5 weeks or older, or preferably 8 weeks of age or older, and preferably in 2 doses, each administered about 3 weeks apart. A single dose may be sufficient if given to a dog at least 12 weeks of age. Dogs can be revaccinated annually with a single dose. Where B. bronchiseptica and/or canine virus exposure is likely, such as breeding, boarding, and showing situations, an additional booster may be given within 1 year, or preferably 6 months, 10 of the occurrence of these events. Another preferred combination vaccine includes a p68 antigen, preferably the recombinant p68 antigen having SEQ ID NO: 1, in combination with an attenuated strain of CPI. Still another preferred combination vaccine includes a p68 antigen, preferably the recombinant p68 antigen having SEQ ID NO: 1, an attenuated strain of CPI, and two at least 15 two Leptospira species such as Leptospira canicola (e.g., strain C-51, National Veterinary Service Laboratory, Ames, IA), and Leptospira icterohaemorrhagiae (e.g., strain NADL 11403, National Veterinary Service Laboratory, Ames, IA). The amount of the p68 antigen and the antigen(s) from one or more other pathogens in the combination vaccines of the present invention should be immunizing-effective. In 20 general, the p68 antigen in a combination vaccine should be in an amount of at least about 0.5 pg per dose. The attenuated CD virus should be in an amount of at least about 102 to about 109 TCID50 per dose TCID 50 (tissue culture infectious dose 50% cytopathic effect) per dose, and preferably in the range of about 104 to about 106 TCID 50 per dose. The attenuated CAV-2 should be in an amount of at least about 102 TCIDo to about 10' TCID 50 per dose, preferably 25 in the range of 1040 to about 106.0 TCID 50 per dose. The attenuated CPI virus should be in an amount of at least about 102 TCID 5 oto about 10 9
TCID
50 per dose, and preferably in the range of 108 to about 108 TCID 5 o per dose. The attenuated CPV should be in an amount of at least about 102 TCID 50 to about 109 TCID 50 per dose, preferably, an amount in the range of 107 to about 10 9
TCID
5 0 per dose. The amount of CCV in an inactivated viral preparation should be 30 at least about 100 relative units per dose, and preferably in the range of 1000-4500 relative units per dose. Each Leptospira species in the vaccine should be in the range of about 100 3500 NU (nephelometric units) per vaccine dose, and preferably in the range of 200-2000 NU per dose. The combination vaccines are formulated such that the vaccines can be administered 35 to dogs by injection in a dose of 0.1 ml to 5 ml, preferably from 0.5 ml to 2.5 ml, and more preferably, about 1 ml.
17 5 The present invention is further illustrated by the following non-limiting examples. EXAMPLE 1 CANINE BORDETELLA p68 RECOMBINANT ANTIGEN DOSE TITRATION STUDY 10 VACCINE: The experimental vaccine antigen was a recombinant p68 outer membrane protein (SEQ ID NO: 1) of B. bronchiseptica produced by E. coli strain LW68. The vaccine contained varying levels of SDS (sodium dodecyl sulfate) solubilized p68, adjuvanted with 50 gg of QAC 15 (Quil A/50gg cholesterol) in a 1 mL dose. CHALLENGE MATERIAL: An aerosol of Bordetella bronchiseptica, dog isolate #85B, passage #3, lot #051597, was used as the challenge material. The mean plate count was 1.59 X 108 CFU/ml. 20 ANIMALS: Sixty male and female canine pups were randomly allocated to one of six treatment groups (10. pups per group). Pups were bled and tracheal swabs were taken 41 days prior to the first vaccination and again 28 days prior to first vaccination and any seropositive or culture positive animals were removed from the study. 25 Animals were randomly assigned to treatments and rooms according to a randomized complete block design. Post-vaccination observations were done without knowledge of vaccine assignment groups. DESIGN: Group Dose Level Route' Number of Animals Tol Saline Control (0.9% saline as a SC 9 1 mL dose) T02 1 pg p68 SC 8 T03 4 jig p68 SC 8 T04 16 pg p68 SC 9 TOS 64 jig p68 SC 9 T06 256 pg p68 SC 9 30 SC = subcutaneous 18 5 PROCEDURE: IVP Administration: Animals were vaccinated on Day 0 with either the placebo or the experimental vaccine. A second vaccination was administered on Day 21. The first vaccination was administered subcutaneously in the right neck and the second vaccination was administered 10 subcutaneously in the left neck. Challenge Administration: All animals were challenged 28 days after the second vaccination with an aerosol of Bordetella bronchispetica. Animals were monitored for coughing for a period of 30 minutes, twice daily (once in the a.m. and once in the p.m.) on days two through fourteen following 15 challenge (Days 51 through 63). Observations and Samples Collection: All injection sites were palpated and measured three dimensionally for seven days following each vaccination (Days 0 through 7 and 21 through 28) and on the 14 th day post each vaccination (Days 14 and 35). 20 Rectal temperatures were recorded on the day of vaccination and for three days following each vaccination (Days 0 through 3 and 21 through 24). Blood was collected on the days of vaccination (Days 0 and 21) and on Days 42, 50, and 63 and assayed by ELISA for specific antibodies against the p68 protein purified from B. bronchispetica. Blood was also collected on Days 42, 49, 50, 52, 54, 56 and 58 and analyzed 25 for Serum Amyloid A (SAA). All animals were tracheal swabbed for B. bronchispetica isolation and blood was collected for B. bronchispetica agglutination titers prior to vaccination (at the vendor, on Day 41 and Day -28) and on Day 49. Bordetella P68 Doq and Mouse Antibody Titration DAB ELISA 30 Purified native p68 was diluted to 600 ng/mL in 0.01 M Borate Buffer and was added to each well at 100 Uwell. The plates were incubated overnight at 4*C. The plates were then washed once with excess PBS-Tween 20. 1% nonfat dried milk in PBS was added to the plates at 200 Uwell. The plates were then incubated for 1 hour at 37 0 C. The plates were then washed once with excess PBS-Tween 20. 35 Dog or mouse serum was added at a 1:50 dilution to the top row of the ELISA plates and two fold serially diluted serum was added all the way down the plate. The plates were incubated for 1 hour at 370C. Subsequently, the plates were washed 3 times with excess PBS-Tween 20. To plates incubated with dog serum above, peroxidase labeled goat anti-dog IgG (H + 40 L), diluted at a 1:2000 dilution, was added at 100 gUwell. The plates were then incubated for 1 hour at 37 0 C. To plates incubated with mouse serum above, peroxidase labeled goat anti mouse IgG (H + L), diluted at a 1:4000 dilution, was added at 100 pUwell. The plates were then incubated for 1 hour at 37"C. The plates were then washed 3 times with excess PBS Tween 20.
19 5 ABTS substrate was added at 100 iUwell. Approximately 20 minutes later, the plates were read with a Molecular Devices or an equivalent plate reader at 405-490 nm. DATA ANALYSIS: Treatment differences in the number of dogs coughing were tested using Fisher's Exact Test. The 5% level of significance was used. 10 ELISA titers were log transformed prior to analysis using a general linear mixed model. The 95% level of confidence was used to assess treatment differences. Challenge observations were monitored twice daily for 30 minutes each. RESULTS: Tracheal Swab Culture and Aglutination Titers 15 Tracheal swab cultures and agglutination titers were evaluated to monitor the B. bronchiseptica status of animals enrolled in the study. A number of dogs demonstrated increased titers at various time points but no titer increased above 128 prior to challenge. Injection Site Observations Injection site reactions following the first vaccination are presented in Table 1. The 20 largest injection site reactions were observed in T05 (64 pg) vaccinated animals, with the largest mean injection site reaction measuring only 14.69 cm 3 (two days post vaccination). T03 (4 ig), T04 (16 Ig) and T06 (256 jig) vaccinated animals demonstrated varying injection site reactions up to 7 days post vaccination. T02 (1 lig) vaccinated animals only demonstrated reactions on Day 1 post vaccination. By the seventh day post vaccination, there was no 25 statistically significant difference in injection site reactions among the treatment groups. By Day 14, all injection site reactions had dissipated. Injection site reactions following the second vaccination are presented in Table 2. Following the second vaccination the largest mean injection site reactions were observed in T06 (256 jig), with the largest mean injection site reaction measuring 50.03 cm 3 (one day post 30 vaccination). Injection site reactions were demonstrated in T05 (64 jig) and T04 (16 jig) animals up to 7 days post second vaccination. Minimal injection site reactions were demonstrated in T03 (4 pg) and T02 (1 pg) animals up to 7 days post vaccination. Injection site reactions that were not statistically different from the placebo group were demonstrated in T02 (1 jig) and T03 (4 gg) post vaccination. Fourteen days post second vaccination no 35 injection site reactions were observed. Frequency of injection site reactions following first vaccination is presented in Table 3. The highest overall LSM frequency, 76%, of injection sites exhibiting a reaction at any time post first vaccination resulted from vaccination with T06 (256 pg). The next most frequent were 72% of the injection sites showing a reaction following the first vaccination with TOS 40 (64 pg), 69% following the first vaccination with T04 (16 jig), and 63% following the first vaccination with T03 (4 pg). The lowest frequency, 38%, followed the first vaccination of T02 (1 g).
20 5 Frequency of injection site reactions following the second vaccination is presented in Table 4. The overall LSM frequency for each vaccine was consistent with that seen post first vaccination. Incidence and duration of injection site reactions following vaccination are summarized in Table 5. The incidence (or the number of dogs showing a reaction at any 10 time) of a measurable injection site reaction was 100% for T03, T04, T05 and T06 (4 pg, 16 pg, 64 jig, and 256 pg, respectively) following the first and second vaccination. Animals that received T02 (1 Vg) demonstrated the least incidence of injection site reactions post vaccination (57.1%). Duration of the reaction (expressed as a least squares means of days with a reaction 15 shown in Table 5) was longer for T04, TOS and T06 (16 jg, 64 lig, and 256 lig, respectively) vaccinated animals following the first and second vaccinations (2.7 to 5.1 days post first vaccination and 6.0 to 6.7 days post second vaccination). T02 and T03 (1 pg and 4 pg, respectively) vaccinated animals demonstrated the fewest number of days with an injection site reaction following the first and second vaccinations (0.3 and 1.3 days post first vaccination 20 and 1.9 and 4.5 days post second vaccination). Rectal Temperatures Mean rectal temperature measurements are summarized in Table 6. The LSM rectal temperature for T02 (1 jig) on Day 1 and 24, for T03 (4 jg) on Day 1, 21, and 24, for T04 (16 pg) on Day 2 and 24, for TOS (64 jg) on Day 23, and for T06 (256 jg) on Day 0, 1, and 24 25 were significantly different from the placebo. On Day 23 all comparisons were not statistically significant (P>0.05) from the placebo. p68 ELISA Serology Summary of p68 ELISA data are presented in Table 7. The pre-vaccination geometric mean virus titers of p68 ELISA specific antibodies in all groups were low (range 30 24.9 to 28.9) and titers for the placebo remained low throughout the duration of the study. Twenty-one days following the first vaccination, p68 ELISA geometric mean titers had increased in the vaccinated treatment (range 55.2 to 4,411.7), however T02 (1 jg) titer was not statistically different from the Placebo (T01). Forty-two days after the second vaccination, geometric mean titers were further increased in all vaccinated groups (range 674.6 to 35 48,382.0) demonstrating good serological response to vaccination. Serum Amyloid A (SAA) Seroloav SAA titers are summarized in Table 8. Prior to challenge, geometric mean SAA titers were low in all the treatment groups (range 0.1 to 0.5). Post challenge, T01 GMT titers ranged from 1.5 to 146.0, where p68 treatment groups ranged from 0.3 to 23.1. All treatment groups 40 were statistically different than the placebo on Days 50, 52, 54, and 56. No statistical differences were demonstrated among the p68 vaccines with the exception of T02 (1 jig) on Day 52 when it demonstrated a statistically different geometric mean from all other p68 treatment groups.
21 5 Challenge Response Challenge response data are presented in Table 9. The response was determined by monitoring coughing following challenge and the observations were analyzed using two methods: least square mean number of days with cough and two consecutive days of 10 coughing (Incidence of Disease). Analysis of the mean number of days coughing demonstrated no statistically significant difference between the p68 treatment groups; but in the dogs vaccinated with placebo coughed a mean of 8.6 days whereas dogs administered p68 vaccines coughed significantly less, means ranging between 2.2 to 4.7 days. 15 When dogs were evaluated using Incidence of Disease, all T01 (placebo) were observed coughing for two consecutive days (100% Incidence of Disease). T04 (16 jg) and T05 (64 pg) vaccinated dogs demonstrated an Incidence of Disease of 55.6% and 66.7%, respectively. Only 28.6% of T02 (1 pg), 50% of T03 (4 lag), and 33.3% of T06 (256 pg) vaccinated dogs were observed coughing for two consecutive days. 20 DISCUSSION: In this study, the objective was to establish a relationship between antigen dose, immune response, and protection in dogs. The p68 antigen doses examined were 1 jig, 4 jig, 16 pg, 64 ig, and 256 jg. Analysis of injection site reaction measurements demonstrated a negligible reaction in 25 the p68 treatment groups, with the exception of T06 (256 jig) on the first day post second vaccination. Reactions that were observed tended to be small, generally decreasing in size during the observation periods. The size of these reactions was clinically insignificant and would most likely go unnoticed on unshaven dogs. Rectal temperatures post vaccination were unremarkable and were within normal 30 limits for all dogs in all groups. Serological response to vaccination was excellent in T03 through T06 groups. In these treatment groups, all demonstrated significantly higher p68 ELISA titers when compared to the placebo from Day 21 through Day 63. T02 (1 jig) demonstrated significant p68 ELISA titers compared to the T01 (placebo) from Day 42 through Day 63. The highest titers were 35 observed in T05 (64 jig) and T06 (256 jig). Examination of the SAA response in all p68 vaccinated dogs following challenge indicated a much smaller rise in the SAA post-challenge when compared to control dogs. No difference was demonstrated between the p68 vaccine dose levels post-challenge with the exception of T02 (1 pg) on Day 52 that demonstrated a statistically different geometric mean 40 from all other p68 treatment groups. Post challenge coughing observations were analyzed using least squares means (LSM) of the number of days with cough or two consecutive days coughing (incidence of disease). Using LSM of days coughing, a significant difference was demonstrated between placebo and all p68 vaccinated groups although no difference was demonstrated between the 22 5 different p68 vaccine dose levels. Using the Incidence of Disease T02 (1 ug), T03 (4ug) and T06 (256ug) vaccinated dogs coughed significantly less than the placebo. CONCLUSIONS: The study was conducted to establish relationship between antigen dose, immune response, and protection in dogs. The p68 antigen doses examined were 1 jg, 4 Ig, 16 pg, 10 64 pg, and 256 pg. All vaccines were safe as demonstrated by minimal injection site reactions, normal rectal temperatures and absence of adverse response to vaccination. The size of the injection site reactions and the duration of these reactions were less in the lower antigenic treatment groups. Serological response to vaccination as measured by ELISA titers was excellent with 15 the higher antigenic dose groups demonstrating higher serological responses. When using LSM days coughing as a method of comparison, all treatment groups demonstrated a significant reduction in coughing when compared to placebo. No differences were noted between the treatment groups. When two consecutive days coughing (or Incidence of Disease) was used for comparison, T02 (1 jg), T03 (4 jg) and T06 (256 jg) vaccinated dogs 20 coughed significantly less than the placebo.
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29 EXAMPLE 2 CANINE BORDETELLA p68 IMMUNOGENICITY STUDY Animals 5 Forty-five male and female mixed breed dogs were purchased. A MLV parvovirus vaccine was administered to all puppies on the day the puppies arrived at the study site. No other vaccines, other than the experimental products, were administered to the puppies during the study. Dogs were approximately 9 weeks of age (± 1 week) on Day 0 (day of first vaccination). 10 Dogs were kept in an isolation facility necessary to prevent exposure to Bordetella and other canine pathogens prior to challenge. After aerosol challenge with Bordetella, isolation procedures were continued to prevent exposure to other canine pathogens. Vaccines 15 Sterile saline was used as a placebo vaccine in treatment groups T01 and T02. Canine recombinant p68 Bordetella'Bronchiseptica Vaccine was used in treatment groups T03 and T04. The structural gene of the p68 antigen was cloned in Escherichia coli and expression of the gene was regulated by a temperature sensitive promoter. The cells were lysed and the inclusion bodies were separated by centrifugation. The recombinant p68 in the 20 inclusion bodies was solubilized by SDS treatment. The recombinant p68 (15 pg per mL) was combined with 50 pg of Quil A and 50 pg of cholesterol per mL in sterile saline as the diluent. Each one mL dose contained 0.28% of ethanol and 0.01% thimerosal. Challenge Inoculum 25 Bordetella bronchiseptica Bihr Cat strain was prepared as the challenge inoculum using the method currently employed by Biologics Control Laboratories-Microbiology. Bordet Genou agar plates were plated with a confluent growth of Bordetella bronchiseptica - Bihr Cat strain and incubated for 48 hours at 37.5 +/- 2.5"C. Virulent phase I colonies were selected and streaked on Bordet-Genou agar and incubated for 24 hours at 37.5 +/- 2.5*C. After 30 incubation, Bordetella saline was used to wash colonies from the agar and the antigen was diluted to an optical density of 0.80 at 600 nm. A cell count was performed pre- and post challenge for confirmation of the nephelometer reading. Challenge target concentration was approximately 1 X 10 9 CFU. The pre-challenge concentration was 2.37 x 109 CFU (100% Phase 1) and post-challenge concentration count was 1.35 x 109 CFU (100% Phase 1).
30 Study Design Summary Table 5 Treatment Treatment Route Number of Animals Group T01 Saline Control Intramuscular 8 T02 Saline Control Subcutaneous 7 T03 p68 15 g/dose Subcutaneous 15 T04 p68 15 ig/dose Intramuscular 15 Randomization/Blinding For the time period from vaccination to day of challenge, animals were assigned to treatments according to a generalized block design. Treatments were randomly assigned to 10 rooms. On the day of challenge, animals were randomly assigned to challenge rooms by block. Qualified individuals, unaware of the assigned treatment groups, conducted microbiological and serological assays and assessments of injection sites, measurements of rectal temperatures, and observations of coughing. 15 Data Analysis Post vaccination response variables consisted of injection site data, rectal temperatures and p68 ELISA titers. Injection site data was summarized in the following ways: 1) number of animals having a measurable reaction by treatment and day of study, 2) number 20 of animal time points having a measurable reaction by treatment, 3) number of animals having a measurable reaction at any time point by treatment. Separately for first and second vaccinations, injection site volume (cubic cm), rectal temperatures and natural log transformed p68 ELISA titer data was analyzed using a general linear mixed model. 25 A priori linear contrasts of the treatment by observation time-point least squares mean were. constructed to test treatment group differences at each observation time-point and to compare time-points within each treatment. The 5% level of significance was used for all comparisons. Post challenge response variables consisted of daily coughing observations, p68 30 ELISA titers and serum amyloid A titers. Number of days coughing during the post challenge period was analyzed using a general linear mixed model. A priori contrasts of the treatment least squares mean was constructed to test treatment group differences. The 5% level of significance was used for all comparisons. Separately for first and second vaccinations, Fisher's Exact test was used to compare 35 treatment groups for the incidence of two days of consecutive coughing. The 5% level of significance was used for all comparisons. For serum amyloid A (SAA) titer data post challenge, the natural log transformation was applied to titer values prior to analysis using a general linear mixed model.
31 A priori linear contrasts of the treatment by observation time-point least squares mean was constructed to test treatment group differences at each observation time-point and to compare time-points within each treatment. The 5% level of significance was used for all comparisons. 5 Study Procedure Detailed Animal Procedures Forty-five (45) seronegative and culture negative pups were randomly assigned to one of 4 treatment groups. Eight and seven dogs were allocated to the intramuscular (IM) or 10 subcutaneous (SC) control groups respectively, for a total of 15 control dogs. Fifteen dogs were allocated to the p68 SC treatment group and 15 dogs were allocated to the p68 IM treatment group. Treatment Groups are detailed in the Study Design Section above. Day 0 was designated as the day of first vaccination. Vaccinations were administered on Day 0 and repeated 21 days later. For the first vaccination, the right side of the neck was 15 used and for the second vaccination, the left side of the neck was used. Intramuscular injections were administered in the right and left semimembranosus muscle for the first and second vaccinations, respectively. All injection sites were measured three dimensionally for seven days following each vaccination with a follow-up measurement conducted 14 days following vaccination. Rectal temperatures were monitored on the day of vaccination (prior to 20 vaccination) and for three days following each vaccination. On Day 35, all animals were tracheal swabbed for B. bronchiseptica culture and blood was collected for agglutination titers. All animals were negative by tracheal swab and serologically negative to Bordetella and deemed eligible for challenge. On Day 45, twenty-four days after the second vaccination, an aerosol challenge of B. 25 bronchiseptica was administered to all dogs. Sedated dogs were challenged using a disposable nose cone, which was fitted snugly over the muzzle of the sedated dog. The nose cone was attached to a nebulizer which was attached to a vacuum pressure pump set at 5.5 to 6.0 psi. One mL of challenge material was placed in the nebulizer and the aerosolized challenge material was administered to each dog for 4 minutes. Personnel making 30 observations were unaware of treatment group assignments. Animals were monitored for coughing for 14 days following challenge (Days 46-59). Observations were made in 2 (two), approximately 30-minute periods at approximately the same time each day, one conducted in the AM and one in the PM and results were recorded. 35 Blood Collection Blood for agglutination titers was collected prior to first vaccination and prior to challenge. Blood for anti-p68 ELISA evaluation was collected prior to vaccination on Days 0 and 21 and on Days 35, 45, and 59. Blood for Serum Amyloid A (SAA) assay was collected on the day of challenge (Day 45) and on Days 46, 48, 50, 52 and 54.
32 Tests Performed on Samples Tracheal swabs were evaluated for the presence of B. bronchiseptica by culture. Each tracheal swab was streaked onto a Bordetella Selective Agar plate. Positive and negative controls were included. The plates were incubated at 37.5 ± 2.50 C for 48 ± 4 hours. 5 The resulting colonies on each plate were compared to the positive control and any colony which appeared identical to the positive control was further tested to confirm the presence of B. bronchiseptica. Confirmational testing included the use of TSI, Citrate and Urea Agar and Nitrate Red media. Sera were evaluated for agglutination titers, p68 ELISA analysis or SAA analysis using 10 the following methods: Agglutination titers - Sera were serially diluted in microtiter plates using Bordetella saline. Positive and negative controls were included on each plate. B. bronchiseptica Strain 87 (grown on Bordet Genou agar, harvested, inactivated and diluted to 20% T at 630nm) was used as the agglutinating antigen and was added to each well. Plates were shaken and 15 incubated at 35 ± 20 C for 2 hours. Plates were read after a second incubation at room temperature for 22 hours. The endpoint titer was determined using the last well to show 50% agglutination. p68 ELISA titers - The recombinant p68 antigen was captured on a 96 well microtiter plate coated with a polyclonal antiserum specific to the Bordetella p68 antigen. Serial two-fold 20 dilutions of the canine serum were added to the plate and incubated. Positive and negative controls at a 1:1000 dilution were included on each plate. A peroxidase labeled affinity purified goat anti-dog IgG indicator conjugate was used to detect antibodies specific for the p68 antigen. A chromogenic substrate ABTS was then added and the plate read when the positive control wells had an O.D. of 1.2 + 0.2. The titer of a given sample was calculated as 25 the reciprocal of the last dilution with an optical density greater than the mean of the negative control serum dilution plus five standard deviations. SAA titers - The canine Serum Amyloid A titers were evaluated using a kit purchased from Accuplex Co., University of Nebraska Medical Center, Omaha, NE 68198. Briefly, canine SAA was captured on a microtiter plate coated with a monoclonal anti-canine SAA antibody. 30 Diluted samples of the canine serum were added to the plate followed by a biotin labeled anti canine antibody conjugate. Following incubation, a peroxidase conjugated streptavidin chromogenic substrate was added. The plate was read after 30 minutes. Results 35 Rectal Temperatures Summary of rectal temperature measurements are presented in Tables 10 and 11.
33 Table 10: Least squares mean of rectal temperatures (C*) in dogs following saline or p68 Bordetella vaccination (post first vaccination) Rectal Temperatures (C*) Treatment Day of Study Std Error 0 1 2 3 T01 saline IM 38.2 38.2 38.2 38.2 0.08 T02 saline SC 38.0 38.3 38.2 38.2 0.09 T03 p68 SC 38.1 38.3 38.1 38.0 0.06 T04 p68 IM 38.2 38.4 38.2 38.2 0.06 5 " First vaccination administered on Day 0. Table 11: Least squares mean of rectal temperatures (C*) in dogs following saline or p68 Bordetella vaccination (post second vaccination") Rectal Temperatures (C*) Treatment Day of Study Std Error 21 22 23 24 T01 saline IM 38.1 38.3 38.2 38.2 0.10 T02 saline SC 38.5 38.5 38.2 38.2 0.10 T03 p68 SC 38.0 38.3 38.1 38.1 0.08 T04 p68 IM 38.1 38.4 38.4 38.4 0.08 10 a Second vaccination administered on Day 21. No significant difference was noted between any groups on any day following the first vaccination. A significant difference was noted between saline vaccinated dogs and all p68 vaccinated dogs (p=0.0053 for T01T02 v T03T04) on Day 21. A significant difference 15 (p=0.01 24) between p68 SC and IM vaccinates was demonstrated on Day 24. Injection Site Reactions Injection site reactions are summarized in Tables 12 and 13. Due to technical oversight, no Injection site observations were conducted at the 14-day observation following 20 the second vaccination (Day 35). Measurable site reactions were observed in T03 (p68 SC) and were minimal in size. A small injection site reaction was noted in one dog in T04 (p68 IM) on Day 3 but the minimal impact of the measurement Is not reflected in the overall mean for the group. 25 Table 12: Least squares mean (cubic cm) of injection site reactions In dogs following saline or p68 (15pg/dose) Bordetella vaccination (post first vaccination) Mean Size (cubic cm) of Injection Site Reactions TreatmentP Day of Study 1 2 3 4 5 6 7 14 T01 saline IM 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 T02 saline SC 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 T03 p68 SC 7.4 4.3 6.8 3.4 2.5 2.6 1.9 0.1 T04 p68 IM 0.0 0.0 0.0c 0.0 0.0 0.0 0.0 0.0 a First vaccination administered on Day 0 b standard error for T01= 0.80, T02=0.84, T03=0.70, and T04=0.70 30 one dog had a small site reaction (0.5cm 2 ) but due to rounding, the minimal impact of value is not reflected in overall mean.
34 Table 13. Least squares mean (cubic cm) of injection site reactions in dogs following saline or p68 (15 ig/dose) Bordetella vaccination (post second vaccinations) Mean Size (cubic cm) of Injection Site Reactions TreatmentP Day of Study 22 23 24 25 26 27 28 TO1 saline IM 0.0 0.0 0.0 0.0 0.0 0.0 0.0 T02 saline SC 0.0 0.0 0.0 0.0 0.0 0.0 0.0 T03 p6 8 SC 8.6 9.2 9.5 7.3 5.2 -4.7 5.1 T04 p68 IM 0.0 0.0 0.0 0.0 0.0 0.0 0.0 a Second vaccination administered on Day 21 5 b standard error for T01=1.12, T02 = 1.19, T03 = 0.92, and T06 = 0.92 Significant differences in injection sites measurements are summarized in Table 14. A significant difference in injection site measurements between T02 (saline SC) versus T03 (p68 15 g SC) was noted for six days following the first vaccination. By Day 7 and again on 10 Day 14 (the two week re-evaluation observation), no significant differences were noted between any treatment group. A significant difference in injection site measurements between T02 (saline SC) versus T03 (p68 15p g SC) was noted for seven days following the second vaccination. Table 14. Significance values for a prior contrasts among least squares 15 mean of injection site reaction measurements. Day of Study Contrasts p-value (treatment v. treatment) 1 T02 v T03 0.0001 T03 v TO4 0.0001 T01T02 v T03T04 0.0001 2 T02 v T03 0.0007 TO3 v TO4 0.0008 T01T02 v T03TO4 0.0101 3 T02 v T03 0.0001 T03 v TO4 0.0001 T01T02 v T03TO4 0.0002 4 T02 v T03 0.0044 T03 v T04 0.0042 TO1T02 v T03T04 0.0339 5 T02 v TO3 0.0313 T03 v TO4 0.0253 22 T02 v T03 0.0001 T03 v TO4 0.0001 T01T02 v T03TO4 0.0002 23 T02 v T03 0.0001 T03 v TO4 0.0001 T01T02 v T03TO4 0.0001 24 T02 v T03 0.0001 T03 v T04 0.0001 T01T02 v T03T04 0.0001 25 T02 v T03 0.0001 T03 v TO4 0.0001 T01T02 v T03T04 0.0013 35 26 T02 v T03 0.0013 TO3 v TO4 0.0007 T01T02 v T03T04 0.0172 27 T02 v T03 0.0035 T03 v TO4 0.0019 T01T02 v T03T04 0.0313 Only significant (P<0.05) contrasts are presented. 1)68 ELISA Titers p68 ELISA data are summarized in Table 15 and Figure 1. Due to the considerable 5 titer response to p68 in the vaccinated dogs, various titration minimums were used at different time-points In the study. Titrations for Days 0 and 21 were started at 50. For Days 35, 45 and 59, titrations were begun at 200. Any value reported as "less than" was divided by 2 prior to analysis. The incremental rise observed in p68 ELISA values for control groups (T01 and T02) during the course of the study is due to these minimum titration values. Agglutination 10 titers remained <4. All p68 vaccinated animals demonstrated at least a four-fold Increase In titers from the first day of vaccination to the day of challenge (Day 0 vs. Day 45) when compared to placebo vaccinated animals. 15 Table 15. Geometric mean and standard errors of p68 (15 pg/dose) ELISA endpoint titers" in dogs following saline or p68 (15 pgdose) vaccination and following B. bronchiseptica aerosol challenge. Treatment Day of Studvb 0 Std 21 Std 35, Std 45 Std 59 Std error error error error error T01 saline IM 25.0 4.78 25.0 4.78 125.4 23.96 100.0 18.14 130.0 32.24 T02 saline SC 25.0 5.06 25.0 5.06 113.5 23.00 100.0 19.39 100.0 26.50 T03 p68 SC 25.0 3.93 189.5 29.77 10688.7 1679.20 4555.1 603.43 8796.0 1592.43 T04 p68 IM 25.0 3.93 121.4 19.08 11023.7 1731.85 4419.2 585.44 13718.1 2483.54 a Titrations for Days 0 and 21 were started at 50. Titrations for Days 35, 45 and 59 20 were stared at 200. Any value reported as "less than" was divided by 2 prior to analysis. b First vaccination occurred on Day 0; Second vaccination occurred on Day 21 and challenge was administered on Day 45. Significant differences for least squares mean of post vaccination and post challenge 25 ELISA titers are listed in Table 16. No significant difference was noted between the p68 SC and p68 IM vaccinated animals after vaccination was complete.
36 Table 16: Significance values for a priori contrasts among least square mean of post vaccination and post challenge p68 ELISA endpoint titers. Day of study Contrast p-value 21 TO1 v T04 0.0001 T02 v T03 0.0001 T03 v TO4 0.0491 35 T01 vTO4 0.0001 T02 v T03 0.0001 45 T01 vTO4 0.0001 T02 v T03 0.0001 59 T01 v T04 0.0001 T02 v TO3 0.0001 5 Only significant (P<0.05) contrasts are presented. Serum Amyloid A Titers SAA values were determined on Days 0, 1, 3, 5, 7 and 9 following challenge. Serum Amyloid A values are presented in Table 17 and represented in Figure 2. 10 Table 17: Geometric mean and standard errors of Serum Amyloid A titers in saline and p68 (15 pg/dose) Bordetella vaccinated dogs following aerosol challenge with Bordetella bronchiseptica Geometric Mean and Standard Errors of Serum Amyloid A Dav of Studva 45 46 48 50 52 54 TRT Mean Std. Mean Std. Mean Std. Mean Std. Mean Std. Mean Std. error error Error error error error T01 0.1 0.02 277.3 125.30 82.4 42.17 69.8 28.60 6.0 2.24 1.2 0.33 T02 0.4 0.06 384.5 185.63 128.9 70.46 215.9 94.56 28.0 11.23 3.7 1.11 T03 0.5 0.05 31.7 10.51 6.0 2.25 2.3 0.68 0.8 0.23 1.3 0.27 T04 0.3 0.04 50.0 16.58 , 8.6 3.22 5.0 1.50 2.4 0.65 0.7 0.14 15 'Challenge administered on Day 45 Significant differences in SAA titers are summarized in Table 18. Saline controls demonstrated higher SAA titers than vaccinated dogs on Days 1, 3 and 5 following challenge. Saline SC controls continued to demonstrate significantly higher SAA titers when compared to 20 vaccinated SC dogs on Day 7 and 9 following challenge.
37 Table 18. Significance values for a priori contrasts among least square mean of Serum Amyloid A titers postchallenge. Day of study Contrast p-value (treatment v treatment) 46 TO1 vTO4 0.0025 T02 v T03 0.0001 48 T01 vTO4 0.0007 T02 v T03 0.0001 50 TO1 v T04 0.0001 T02 v T03 0.0001 52 TO1 v T02 0.0093 T02 v T03 0.0001 54 T02 v T03 0.0493 5 Only significant (P<0.05) contrasts are presented. Coughing Observations Aerosol challenge for all treatment groups occurred 24 days following the second 10 vaccination (Day 45). Coughing observations were examined using two methods - disease status based on two consecutive days coughing (presented in Table 19) and percentage of days coughing (presented in Tables 20 and 21). When dogs were evaluated using criteria of two consecutive days coughing, 80% of the p68 vaccinated dogs (SC and IM) coughed at least two consecutive days whereas the Saline SC and Saline IM vaccinated dogs coughed 15 100% and 87.5%, respectively. When dogs were evaluated using percentage of days observed coughing, p68 SC and IM vaccinated dogs coughed 38.72% and 41.05% of the days observed, respectively. Saline SC and Saline IM vaccinated dogs coughed 69.04% and 62.66%, respectively. Table 19. Summary of disease status in saline and p68 (15 gg/dose) Bordetella 20 vaccinated dogs based on two consecutive days coughing following aerosol challenge with Bordetella bronchiseptica Treatment Number # of Dogs Percent Dogs with Two Consecutive Days Coughing T01 Saline IM 8 87.5 T02 Saline SC 7 - 100.0 T03 p68 15 sg/dose SC 15 80.0 T04 p68 15 g/dose IM 15 80.0 No significant difference was demonstrated between saline vaccinated and p68 25 vaccinated dogs when disease status was based on two consecutive days coughing.
38 Table 20. Mean percentage of days coughing in saline and p68 (15 pg/dose) Bordetella vaccinated dogs following aerosol challenge with Bordetella bronchiseptica Treatment Number of Mean Std Error Dogs T01 Saline IM 8 62.66% 8.67 T02 Saline SC 7 69.04% 8.86 T03 p6815 pg SC 15 38.72% 6.38 T04 p6815 pg IM 15 41.05% 6.44 A significant difference (p= 0.0112) was demonstrated between T02 (saline SC) and 5 T03 (p68 15 gg SC). No significant difference was demonstrated between T01 (saline IM) and T04 (p68 15 g IM). Table 21. Mean percentage of days coughing by treatment In saline and p68 (15 pg/dose) Bordetella vaccinated dogs following aerosol to challenge with Bordetella bronchiseptica Parameter Estimate Std error Saline mean (T01 &T02) 65.89% 1.10 15 pg/dose mean (TO3 & T04) 39.88% 4.34 A significant difference (p=0.0022) was demonstrated between the saline controls and p68-vaccinated dogs. 15 Discussion The study was designed to demonstrate the safety and efficacy of a p68 Bordetella 15 jg/dose vaccine in dogs. Safety was examined using injection site and rectal temperature observations. 20 Analysis of injection site reaction measurements demonstrated a negligible reaction in the IM vaccinated group and minimal reactions in SC vaccinated group. Reactions that were observed tended to be small, generally decreasing in size during the observation periods. The size of these reactions would most likely go unnoticed on unshaven dogs. Although a significant difference was observed between saline and vaccinated on Day 21 and between IM 25 and SC vaccinates on Day 24, rectal temperatures were clinically unremarkable and were within normal limits for all dogs in all groups. Efficacy was examined using observations of measurement of p68 ELISA endpoint titers and coughing. Regardless of the route of administration, a good p68 antibody response was demonstrated in p68-vaccinated groups by Day 35. A good anamnestic response was 30 observed in vaccinates post challenge. Although higher antibody responses have traditionally been obtained with the more vascular and less fatty IM route as compared to the SC route, the difference between p68 SC and IM vaccinates was not significant through the course of the study. Examination of the SAA response in vaccinated and unvaccinated p68 Bordetella 35 dogs following challenge indicated a much smaller rise in the SAA values in vaccinated animals groups especially on days 1, 3 and 5 days post-challenge.
39 Conclusions In this study, efficacy a 15 gg/dose p68 canine Bordetella vaccine was examined using a canine challenge model 24 days after vaccination. The vaccine was safe as demonstrated by normal rectal temperatures, minimal injection site reactions and efficacy was 5 demonstrated in combined IM and SC groups. Comparison of SAA values demonstrated a significant difference between saline and p68 vaccinated dogs on Days 1, 3 and 5 following aerosol challenge. EXAMPLE 3 10 SIX MONTH DURATION OF IMMUNITY STUDY OF CANINE BORDETELLA p68 VACCINE Animals Ninety male and female mixed breed dogs were purchased and the majority of 15 puppies were 9 weeks (±1 week) on the day of first vaccination. A MLV parvovirus vaccine was administered to dogs upon arrival at the study site. To be eligible for the study, animals were determined to be negative to B. bronchiseptica by tracheal swab and agglutination titer. No vaccines, other than the experimental products, were administered during the study. 20 Dogs were kept in an isolation facility necessary to prevent exposure to B. bronchiseptica and canine pathogens prior to challenge. After aerosol challenge with B. bronchiseptica, isolation procedures were continued to prevent exposure to other canine pathogens. 25 Vaccines Sterile saline was used as a placebo vaccine in treatment groups T01 and T02. Canine recombinant p68 Bordetella Bronchiseptica Vaccine was used in treatment groups T03 and T04. The structural gene of the p68 antigen was cloned in Escherichia coi and expression of the gene was regulated by a temperature sensitive promoter. The cells were 30 lysed and the inclusion bodies were separated by centrifugation. The recombinant p68 in the inclusion bodies was solubilized by SDS treatment. Separately, the 15 tg p68 and 60 pg p68 were combined with 50 sg of Quil A and 50 lag of cholesterol per mL in sterile Lepto saline as the diluent. The combined components were mixed at 4 0 C for 24 hours and passed three times through a microfluidizer. Each one mL dose contained 2.7 y1 of ethanol and 0.0001% 35 thimerosal. p68 concentrations in the experimental vaccines were measured by p68 ELISA. All assays were done in replicates of five (5). All vaccines were used within 6 months of assembly. Challenge Inoculum 40 Bordet-Genou agar plates were plated with Bordetella bronchiseptica - Bihr Cat strain and incubated for 48 hours at 37.5 +/- 2.5"C. Virulent phase I colonies were selected and streaked on Bordet-Genou agar and incubated for 24 hours at 37.5 +/- 2.5 0 C. After 40 incubation, Bordetella saline was used to wash colonies from agar and the cells diluted to an optical density of 0.80 at 600 nm. A cell count was performed pre and post challenge for confirmation of the nephelometer reading. Challenge target concentration was approximately 1 X 109 CFU. For Group 1, the prechallenge concentration count was 1.94 X 109 and the post 5 challenge concentration count was 1.43 X 109. For Group 1I, the prechallenge concentration count was 2.55 X 109 and the post challenge concentration count was 2.13 X 109. Study Design Summary Table 10 Number of Animals Treatment Treatment Route Da- of First Vaccination Group Day 0 Day 20 Total Number (Group 1) (Group II) of Animals Tol Saline Control Subcutaneous 8 7 15 T02 Saline Control Intramuscular 8 7 15 T03 p68 60 pqdose Subcutaneous 8. 7 15 T04 p68 60 pg/dose Intramuscular 8 7 15 T05 p68 15 g/dose Subcutaneous 8 7 15 T06 p68 15 pq/dose Intramuscular 8 7 15 The study was conducted in two phases or groups consisting of 48 dogs in Group I and 42 dogs in Group II. Vaccination #1 occurred on Day 0 for the each Group. Vaccination #2 occurred 20 days later. Events in Group 1 were offset from the events in Group 11 by approximately 15 days. 'Dogs were aerosol challenged with B. bronchiseptica 181 days after 15 the last vaccination. Randomization/Blinding Animals were randomly assigned to treatments and rooms according to a complete randomized design. For the time period from vaccination #1 to day of challenge within each study group, 20 animals were randomly assigned to treatments and rooms (3 to 5 dogs per room) using a randomization plan. On the day of challenge, the previously treated animals were randomized to challenge rooms within study group using a generalized block design. Qualified individuals, unaware of the assigned treatment groups, conducted 25 microbiological and serological assays and assessments of coughing and injection sites. Data Analysis . Post vaccination response variables consisted of injection site data, rectal temperatures and p68 ELISA titers. Injection site data was summarized as follows: 1) number of animals having a measurable reaction by treatment and day of study, 2) number of animal 30 time points having a measurable reaction by treatment, 3) number of animals having a measurable reaction at any time point by treatment, 4) duration of a measurable reaction for each animal.
41 Separately for first and second vaccinations, injection site volume (cubic cm), rectal temperatures and natural log transformed p68 ELISA titer data was analyzed using a general linear mixed model. A priori linear contrasts of the treatment by observation time point least squares mean 5 was constructed to test treatment group differences at each observation time point and to compare time points within each treatment. The specific comparisons of interest were T01 vs. T03, T01 vs. T05, T03 vs. T05, T02 vs. T04, T02 vs. T06, and T04 vs. T06. If the time point by-treatment-by-study group interaction term was significant at P<0.05, contrasts among treatment groups at each time point and among time points within treatment groups was within 10 each study group, otherwise these contrasts were based on the time point-by-treatment interaction effect least squares mean. The 5% level of significance was used for all comparisons. Post challenge response variables consisted of p68 ELISA titers,- Serum Amyloid A titers and daily coughing observations. Post challenge p68 ELISA titers were analyzed as 15 previously described. For Serum Amyloid A (SAA) titer data post challenge, the natural log transformation was applied to titer values prior to analysis using a general linear mixed model. The analysis of coughing was amended to reflect USDA requirements. For each dog, the percentage of observation periods during which coughing was observed was calculated. Prior to analysis, the percentage was transformed using the arcsin square root transformation. 20 A general linear mixed model was used for analysis of coughing. Least squares mean from this analysis were back-transformed to percentages and the percent reduction in coughing was calculated as: Percent Reduction = 100 X (control aroup mean - treatment group mean) ' (control group mean) 25 A prior linear contrasts of the treatment least squares mean was constructed to test treatment group differences. The specific comparisons of interest were T01 vs. T03, TO1 vs. T05, T03 vs. T05, T02 vs. T04, T02 vs. T06, and T04 vs. T06. If the treatment-by-study group interaction term was significant at P<0.05, contrasts among treatment groups was within each study group otherwise contrasts among treatment groups were based on the treatment main 30 effect least squares mean. The 5% level of significance was used for all comparisons. Study Procedure Detailed Animal Procedures Prior to arrival on study premises and prior to the first vaccination, puppies were 35 tracheal swabbed for B. bronchiseptica culture and blood was collected for agglutination titers. All animals were negative by tracheal swab and serologically negative to Bordetella and deemed eligible for the study. Forty-eight puppies were randomly assigned to one of six treatment groups for Group I. The procedure was repeated using forty-two dogs for Group 1I. Animals were acclimated to the study site for at least five days.
42 Groups and treatments are detailed in Section 7.4.A. Due to facility constraints and to enhance the accuracy of coughing observations following challenge, the vaccination and the respective challenge periods were staggered by 15 days to generate the two dog groups. Day 0 refers to the day of vaccination #1 for both Group I and 11. Vaccination #2 occurred 20 days 5 later. Treatments T01, T03, and T05 were administered via the subcutaneous route. Treatments T02, T04, and T06 were administered via the intramuscular route. Subcutaneous injections were administered in the dorsolateral aspect of the neck. For vaccination #1, the right side of the neck was used and for vaccination #2, the left side of the neck was used. Intramuscular injections were administered in the right and left semimembranosus muscle for 10 vaccination #1 and vaccination #2, respectively. All injection sites were measured three dimensionally for seven days following each vaccination with a follow-up measurement done 14 days following vaccination. Rectal temperatures were monitored on the day of vaccination (prior to vaccination) and for three days following each vaccination. Blood was collected prior to each vaccination (on Day -1 and Day 19) and on Day 50 for p68 ELISA titer determination. 15 Each month, all dogs were tracheal swabbed for B. bronchiseptica culture under sedation to confirm B. bronchiseptica negative status. Blood was also collected for agglutination and ELISA titers. The procedure was repeated 7 days prior to challenge for each group. Evidence of a positive tracheal swab culture or a rising agglutination titer excluded the animal from the study. 20 Challenge was administered to dogs 181 days after vaccination #2. Sedated dogs were challenged using a disposable nose cone, which was fitted snuggly over the muzzle of the sedated dog. The nose cone was attached to a nebulizer which was attached to a vacuum pressure pump set at 5.5 to 6.0 psi. One mL of challenge material was placed in the nebulizer and the aerosolized challenge material was administered to each dog for 4 minutes. 25 Post challenge coughing observations were amended prior to challenge to comply with USDA recommendations. After challenge, each group of dogs was observed between the third and tenth day following challenge, for a total of 8 days. Animals were observed twice daily for coughing for approximately 45 minutes at each observation period. The interval between observation periods was approximately 12 hours. Personnel unaware of the 30 assigned treatment groups recorded coughing observations. Blood Collection Blood for agglutination titers was collected prior to first vaccination, monthly and prior to challenge for each group. 35 Blood for anti-p68 ELISA evaluation was collected the day before vaccination #1 and #2, on Day 50 and at approximately 30 day intervals thereafter for each group. Blood was also collected the day of challenge and on the final day of post challenge observation. Blood for Serum Amyloid A (SAA) assay was collected on the day of challenge (prior to challenge) and on 1, 3, 5, 7, and 9 days post challenge for each group.
43 Tests Performed on Samples Tracheal swabs were evaluated for the presence of B. bronchiseptica by culture. Each tracheal swab was streaked onto a Bordetella Selective Agar plate. Positive and negative controls are included. The plates were incubated at 37.5 ± 2.5 0 C for 48 ± 4 hours. 5 The resulting colonies on each plate were compared to the positive control and any colony which appeared identical to the positive control was further tested to confirm the presence of B. bronchiseptica. Confirmational testing included the use of TSI, Citrate and Urea Agar and Nitrate Red media. Sera were evaluated for agglutination titers, p68 ELISA analysis or SAA analysis using 10 the following methods: Agglutination titers - Sera were serially diluted in microtiter plates using Bordetella saline. Positive and negative controls were included on each plate. B. bronchiseptica Strain 87 (grown on Bordet Genou agar, harvested, inactivated and diluted to 20%T at 630nm) was used as the agglutinating antigen and was added to each well. Plates were shaken and 15 incubated at 35 ± 2 0 C for 2 hours. Plates were read after a second incubation at room temperature for 22 hours. The endpoint titer was determined using the last well to show 50% agglutination. p68 ELISA titers - The recombinant p68 antigen was captured on a 96 well microtiter plate coated with a polyclonal antiserum specific to the Bordetella p68 antigen. Serial two fold 20 dilutions of the canine serum were added to the plate and incubated. Positive and negative controls at a 1:1000 dilution were included on each plate. A peroxidase labeled affinity purified goat anti-dog IgG indicator conjugate was used to detect antibodies specific for the rp68 antigen. A chromogenic substrate ABTS was then added and the plate read when the positive control wells had an O.D. of 1.2 + 0.2. The titer of a given sample was calculated as the 25 reciprocal of the last dilution with an optical density greater than the mean of the negative control serum dilution plus five standard deviations. SAA titers - The canine Serum Amyloid A was captured on a 96 well microtiter plate coated with a monoclonal anti-canine SAA antibody. Diluted samples of the canine serum were added to the plate and incubated. A reference standard was added to obtain a standard 30 curve from 0.31 ng/ml to 20 ng/ml. A biotin labeled anti-canine antibody conjugate was added. Following the incubation of the biotin labeled anti-canine antibody, a peroxidase conjugated streptavidin was added. A chromogenic substrate TMB was added and the plate was read after 30 minutes. The concentration of Serum Amyloid A was determined by comparison the sample to the standard curve and multiplication by the appropriate dilution factor. 35 Results Unless otherwise noted, results are the combined data from Group I and 11. Tracheal Swab Culture and Aqqlutination Titers Positive tracheal swab cultures and/or rising agglutination titers were demonstrated in 40 eleven dogs during the course of the study. These dogs and any dog housed with the positive dogs were removed from the study, resulting in a loss of 20 dogs. The number of dogs 44 removed from each group was: T01-4 dogs, T02-2 dogs, T03-3 dogs, T04-1 dog, T05-5 dogs, T06-5 dogs. Injection Site Observations Injection site reactions are summarized in Tables 22-25. Injection site information 5 was not collected for Dog 81595 on Day 21 for Group I due to technical oversight. The protocol was amended so that injection site reaction data was not collected for dogs in Group II on Day 22 therefore, summary of data from Day 22 contains only information from the eight dogs per treatment group in Group I. Injection site reactions were not observed for any dog receiving an IM treatment. Injection site measurements were minimal for both SC vaccinated 10 treatment groups (T03 and T05). Table 22. Least squares mean (cubic cm) of injection site reactions in dogs following saline or p68 Bordetella vaccination (post first vaccination') Mean Size (cubic cm) Treatment Day of Study 1 2 3 4 5 6 7 14 TO1 saline SC 0 0 0 0 0 0 0 0 T02 saline IM 0 0 0 0 0 0 0 0 T03 60 pg SC 5.8 6.4 7.3 6.3 5.0 4.9 2.7 0 TO4 60 pg IM 0 0 0 0 0. 0 0 0 T05 15 pg SC 4.4 4.6 3.1 2.1 1.2 0.9 0.7 0 T06 15 pg IM 0 0 0 0 0 0 0 0 15 a Vaccination #1 administered on Day 0 bstandard error for all means = 0.64 20 Table 23. Least squares mean (cubic cm) of injection site reactions In dogs following saline or p68 Bordetella vaccination (post second vaccination) Mean Size (cubic cm) Treatment Day of Study 21 22c 23 24 25 26 27 b 34 T01salineSC 0 0 0 0 0 0 0 0 T02salinelM 0 0 0 0 0 0 0 0 T03 60 gg SC 5.0 3.2 4.2 5.8 5.9 5.8 4.3 0.1 T04 60 g IM 0 0 0 0 0 0 0 0 T05 15 gg SC 3.7 2.4 2.2 2.6 2.1 2.0 1.6 0 T0615 g IM 0 0 0 0 0 0 0 0 "Vaccination #2 administered on Day 20 bstandard error for means on this day = 0.55 25 c standard error for means on this day 0.70 dstandard error for T06 on Day 21 = 0.57 Table 24. Percent of dogs having a measurable injection site reaction following 30 vaccination with saline or p68 Bordetella (post first vaccination') Percent Measurable Reaction Treatment n 1 2 3 4 5 6 7 1 4 T01 saline SC 15 0 0 0 0 0 0 0 0 45 T02 saline IM 15 0 0 0 0 0 0 0 0 T03 60 pg SC 15 66.7 73.3 73.3 73.3 73.3 73.3 66.7 0 TO4 60 ag IM 15 0 0 0 0 0 0 0 0 T05 15 pag SC 15 73.3 73.3 60.0 60.0 53.3 46.7 46.7 0 T06 15 pg IM 15 0 0 0 0 0 0 0 0 aVaccination #1 administered on Day 0 Table 25. Percent of dogs having a measurable injection site reaction following vaccination with saline or p68 Bordetella (post second vaccination) 5 Percent Measurable Reaction Treatment Day of Study n 21 22 b 23 24 25 26 27 34 T01salineSC 15 0 0 0 0 0 0 0 0 TO2salinelM 15 0 0 0 0 0 0 0 0 T03 60 jg SC 15 80.0 50.0 66.7 66.7 80.0 80.0 80.0 6.7 TO4 60 pg IM 15 0 0 0 0 0 0 0 0 T05 15 pg SC 15 73.3 50.0 73.3 73.3 73.3 73.3 66.7 0 T0615 4g IM 15 Oc 0 0 0 0 0 0 0 Vaccination #2 administered on Day 0 b n=8 for Day 22 cn=1 4 for T06 on Day 21 10 Significant differences in injection sites measurements are summarized in Table 26. A significant difference in injection sites measurements between T01 (saline SC) versus T03 (60 pg SC) was noted for seven days after the first vaccination. A significant difference between T01 (saline SC) and T05 (15 ig SC) was noted for only the first four days after the first vaccination. A significant difference was found between T03 (60 jg SC) and T05 (15 g 15 SC) on Days 3 through 7. By Day 14 (the two week re-evaluation observation), no difference was noted between any of the groups. A significant difference (Pi=0.0138) in injection sites measurements between T01 (saline SC) versus T03 (60 pg SC) and T05 (15 jg SC) was noted for seven days after the second vaccination. A significant difference was found between T03 (60 Ig SC) and T05 (15 20 gg SC) on Days 23 through 27. By Day 34 (the two week re-evaluation observation), no difference was noted between any of the groups Table 26. Significance values for a priori contrasts among least squares mean of Injection site reaction measurements. 25 Day of Study Contrasts p-value (treatment v. treatment) 1 TO1 v T03 0.0001 T01 v T05 0.0001 2 TOI vT03 0.0001 T01 v TO5 0.0001 3 T01 v T03 0.0001 TO1 v T05 0.0006 TO3 v T05 0.0001 4 T01 v.T03 0.0001 T01 v TO5 0.0214 TO3 v T05 0.0001 5 T01 vT03 0.0001 T03 v TO5 0.0001 6 T01 v T03 0.0001 T03 v T05 0.0001 46 7 T01 vT03 0.0031 T03 v T05 0.0258 21 T01 vTO3 0.0001 T01 vTO5 0.0001 22 T01 v T03 0.0010 T01 vT05 0.0166 23 T01 vT03 0.0001 T01 vTO5 0.0055 T03 v T05 0.0113 24 T01 v T03 0.0001 T01 vT05 0.0008 T03 v TO5 0.0001 25 T01 vT03 0.0001 T01 vT05 0.0067 T03 v TO5 0.0001 26 T01 vTO3 0.0001 T01 vT05 0.0104 T03 v T05 0.0001 27 T01 vT03 0.0001 T01 vTO5 0.0475 T03 v TOS 0.0004 Only significant (P<0.05) contrasts are presented. Rectal Temperature Rectal temperature measurements are summarized in Tables 27 and 28. The protocol was amended so that rectal temperature data were not collected for Group l1 dogs on 5 Day 22, therefore, summary of data from Day 22 contains only information from the dogs per treatment group in Group 1. Table 27. Least squares mean of rectal temperature ("C) In dogs following saline or p68 Bordetella vaccination (post first vaccination) 10 Rectal Temperature(oC)b Treatment Day of Study 0 1 2 3 T01 saline SC 38.5 38.4 38.3 38.2 T02 saline IM 38.4 38.2 38.4 38.2 T03 60 pg SC 38.4 38.5 38.2 38.3 T04 60 pg IM 38.4 38.5 38.4 38.4 TOS 15 pg SC 38.5 38.5 38.2 38.2 T06 15 pg IM 38.5 38.4 38.3 38.4 a Vaccination #1 administered on Day 20. b standard error = 0.09 Table 28. Least squares mean of rectal temperature (*C) in dogs following 15 saline or p68 Bordetella vaccination (post second vaccination') Rectal Temperature(*C) Treatment Day of Study 202 21 bc T01 saline SC 38.6 38.5 38.3 38.4 T02 saline IM 38.6 38.6 . 38.3 38.4 T03 60 pg SC 38.7 38.6 38.6 38.4 T04 60 gg IM 38.8 38.7 38.5 38.5 T05 15 pg SC 38.6 38.6 38.5 38.4 T06 15 g IM 38.7 38.7 38.4 38.5 a Vaccination #2 administered on Day 20 b standard error = 0.08 C standard error = 0.11 20 47 A significant difference was noted in rectal temperatures between T02 (saline IM) and T04 (60 gg IM) on Day 1 after the first vaccination. No significant difference was found in rectal temperatures between any group on any day after the second vaccination. p68 ELISA Titers 5 Prechallenge p68 ELISA data are summarized in Table 29. Due to the response of Group I dogs in T06 on Day 19, an effect due to group was observed in the data analysis of p68 ELISA titers. The effect was small and did not influence other analyzed timepoints. Therefore, data from Group I and lI are combined for reporting purposes. Due to the considerable titer response to p68 in the vaccinated dogs, various titration 10 minimums were used at different timepoints in the study. Titrations for Days -1 and 19 were started at 50. For days 50 through 195, titrations were begun at 200. Titrations for samples collected on Day 201 and 211 were started at 1000. Any value reported as "less than" was divided by 2 prior to analysis. The incremental rise observed in p68 ELISA values for control groups (T01 and T02) during the course of the study is due to these minimum titration values. 15 Agglutination titers, except as previously noted, remained <4. All placebo vaccinated dogs had p68 titers <200 on Day 50. All p68-vaccinated animals demonstrated at least a four-fold increase in titers after the second vaccination (Day 0 vs. Day 50) when compared to placebo vaccinated animals. 20 Table 29. Geometric mean and standard errors of p68 ELISA endpoint titers in dogs following p68 Bordetella vaccination on Day 0 and Day 20. Day of Study Treatment _,a 19,a 50 b Mean std. error Mean std. error Mean esror Tol Saline SC 31.4 5.06 30.5 4.92 100.0 16.14 T02 Saline IM 27.7 4.47 25.0 4.03 100.0 16.14 T03 P68 60 ug SC 29.8 4.80 507.6 81.92 10633.5 1715.9 4 T04 P68 60 ug IM 26.7 4.31 249.7 40.29 4722.2 762.02 TOS P68 15 ug SC 25.0 4.03 268.7 43.36 5622.8 907.35 T06 P68 15 ug IM 33.4 5.40 91.8 14.81 3528.9 569.46 atitrations for Days -i and 19 were started at 50. Any value reported as "less than" was divided by 2 prior to analysis 25 btitrations for Day 50 were started at 200. Any value reported as "less than" was divided by 2 prior to analysis Significant differences for least squares mean of post vaccination ELISA titers are listed in Table 30. No significant difference in p68 ELISA titers was observed between SC 30 controls and SC vaccinates or IM controls and IM vaccinates prior to vaccination (Day -1).
48 Table 30. Significance values for a priori contrasts among least square mean of post vaccination p68 ELISA endpoint titers. Day of study Contrast p-value 19 T01 v TO3 0.0001 T01 v T05 0.0001 T02 v TO4 0.0001 T02 v T06 0.0001 T03 v T05 0.0061 T04 v T06 0.0001 50 T01 vT03 0.0001 T01 vTO5 0.0001 T02 v TO4 0.0001 T02 v T06 0.0001 T03 v TO5 0.0059 Only significant (P<0.05) contrasts are presented. 5 p68 ELISA titers measured during the course of the study are summarized in Table 31 and illustrated in Figure 3. During the course of the study, every attempt was made to coordinate activities between Groups I and 11. For pivotal data collection time points (i.e. events surrounding 10 vaccination and challenge), this was achieved. In three instances during the interim of the study, blood and tracheal swab collection varied by 1 or 2 days between groups. In order to summarize and report p68 ELISA data for this interim period, data from these days were combined. Therefore, Day 79 contains combined data from Day 79 (Group I) and Day 81 (Group II), Day 111 corresponds to Day 110 (Group II) and Day 111 (Group 1) and Day 169 15 corresponds to Day 169 (Group II) and Day 170 (Group 1). Per the protocol, data analysis was not performed on p68 ELISA data beyond Day 50. Table 31. Summary of geometric mean of p68 ELISA endpoint titers in unvaccinated and p68 Bordetella vaccinated dogs following vaccination and aerosol challenge with 20 Bordetella bronchiseptica Geometric Mean of p68 ELISA Endpoint Titers. Treatment Day of Studv' 79 110 140 169 195 201 211 T01 Saline SC 100.00 154.92 106.00 108.59 117.18 109.14 500.0 T02 Saline IM 112.85 145.33 130.61 115.51 113.43 112.37 500.0 T03 60 ig SC 3434.24 2884.97 1861.64 1895.56 3097.24 2408.61 81564.79 T04 60 pg IM 1508.60 1164.81 933.12 987.64 1142.01 1547.87 59940.47 T05 15 pag SC 1699.20 1511.80 1229.35 1312.62 2248.63 2244.47 29869.29 T06 15 pg IM 974.18 916.21 573.51 876.15 1505.01 1458.16 11503.28 a Titrations Days 50 through 195, were started at 200. Titrations for samples collected on Day 201 and 211 were started at 1000. Any value reported as "less than" was divided by 2 prior 25 to analysis. b Vaccination #1 and #2 was administered on Day 0 and 20, respectively. Challenge was administered on Day 201.
49 c During the course of the study, every attempt was made to'coordinate activities between Groups I and II. In three instances, blood collection for the Groups varied by 1 or 2 days. Data from these days were combined for data summary. Analysis was not done on p68 ELISA titer values collected beyond Day 50. Day 79 corresponds to Day 79 and 81, Day 5 110 corresponds to Day 110 and 111, Day 169 corresponds to Day 169 and 170. Coughing Observations Aerosol challenge for both groups occurred 181 days following the second 10 vaccination. To comply with USDA recommendations, coughing criteria was amended to approximately 45-minute observations, approximately twelve hours apart on the third through eighth day following challenge. Coughing observations are summarized in Tables 32 and 33. Table 32. Mean percentage of timepoints coughing in unvaccinated and p68 Bordetella vaccinated dogs following aerosol challenge with Bordetella bronchiseptica 15 Treatment Number of Mean Std Error Dogs T01 Saline SC 11 75.44% 7.73 T02 Saline IM 13 80.50% 6.64 T03 p68 60 pg SC 12 67.30% 8.12 T04 p68 60 pq IM 14 71.81% 7.32 TOS p68 15 pg SC 10 36.30% 9.19 T06 p68 15 pg IM 10 39.55% 9.18 Table 33. Mean percentage of timepoints coughing by treatment In unvaccinated and p68 Bordetella vaccinated dogs following aerosol challenge with Bordetella 20 bronchiseptica Parameter Estimate Std error Saline mean (TO1 & T02) 78.26% 5.28 60 pg/dose mean (T03 & T04) 69.58% 5.68 15 gg/dose mean (T05 & T06) 37.92% 6.70 The percent reduction in coughing when compared to the saline control was 51.55% for the 15 sg/dose groups and 11.09% for the 60 tig/dose groups. Statistical significant 25 differences are summarized in Table 34. Table 34. Significance values for a priori contrasts among least squares mean for percentage of timepoints coughing Contrast p-value T01 v TO5 0.0041 T03 v TO5 0.0199 TO2 v T06 0.0019 TO4 v T06 0.0119 T01 & T02 v T05 & T06 0.0001 30 (Only significant (P<0.05) contrasts are presented.) Serum Amyloid A SAA values were determined on Days 0, 1, 3, 5, 7 and 9 following challenge. Serum Amyloid A values are presented in Table 35 and represented in Figure 4.
50 Table 35: Geometric mean and standard errors of Serum Amyloid A titers in unvaccinated and p68 Bordetella vaccinated dogs following aerosol challenge with Bordetella bronchiseptica Geometric Mean and Standard Errors of Serum Amyloid A8 TX Day of Study 201 202 204 206 208 210 Mean Std. Mean Std. Mean Std. Mean Std. Mean Std. Mean Std. . error error error error error erroi T01 1.1 0.33 257.3 84.61 371.6 116.44 548.7 171.93 68.0 21.31 9.4 2.95 T02 1.0 0.28 114.0 32.83 111.9 32.21 108.9 31.36 15.6 4.48 1.7 0.49 T03 0.8 0.25 135.7 40.55 119.7 35.75 74.5 22.25 10.9 3.38 1.0 0.30 T04 0.8 0.23 134.7 39.20 156.0 43.59 174.8 48.86 34.6 9.67 1.9 0.54 TOS 0.8 0.28 68.4 22.85 88.3 29.50 9.3 3.12 1.9 0.64 2.0 0.66 T06 0.8 0.27 45.7 14.94 54.0 17.67 9.4 3.07 1.9 0.63 0.8 0.27 5 challenge administered on Day 201. Significant differences in SAA titers are summarized in Table 36. Table 36. Significance values for a priori contrasts among least square mean 10 of Serum Amyloid A titers postchallenge. Day of study Contrast p-value (treatment v treatment) 202 T01 vT05 0.0054 T02 v T06 0.0393 TO4 v T06 0.0154 204 T01 v T03 0.0097 T01 vT05 0.0020 TO4 v T06 0.0154 206 T01 v T03 0.0001 T01 vT05 0.0001 T02 v T06 0.0001 T03 v TO5 0.0001 TO4 v T06 0.0001 208 T01 vT03 0.0001 T01 v TOS 0.0001 T02 v T06 0.0001 T03 v T05 0.0023 T04 v T06 0.0001 210 T01 v T03 0.0002 T01 v T05 0.0068 Only significant (P<0.05) contrasts are presented. 15 Discussion The study was designed to demonstrate the safety and six-month efficacy of a recombinant p68 Bordetella vaccine in dogs. Safety of both the 15 gg/dose and the 60 gg/dose vaccine was demonstrated. The efficacy and 6 month duration of immunity of the 15 gg/dose was well supported in the study. 20 Safety was examined using Injection site and rectal temperature observations. Analysis of injection site reaction measurements demonstrated no reactions in the IM vaccinated groups and minimal reactions in both the 15 pg/dose and 60 pg/dose SC vaccinated groups. Reactions that were observed tended to be smaller in the 15gg/dose SC vaccinated dogs. Such reactions that were seen were transient, generally resolving in 14 days 51 or less. The size of these reactions would most likely go unnoticed on dogs where injection sites were not shaven. Rectal temperatures post vaccination were unremarkable and were within normal limits for all dogs in all groups. Efficacy and duration of immunity were examined 181 days from vaccination using 5 observations of coughing and measurement of p68 ELISA endpoint titers. The percentage of coughing in the saline controls (78.26%) indicated that the challenge administered to the study animals was acceptable. Percentage of time points coughing for the 15 pg/dose group (37.92%) demonstrated a 51.55% reduction in coughing when compared to the controls, satisfying the efficacy requirements mandated by the USDA. No protection was demonstrated 10 in the 60 pg/dose group (69.58%) with dogs demonstrating minimal reduction in coughing when compared to the controls. Although not statistically compared, it can be seen from Tables 29 and 31 that SC vaccinates tended to have higher antibody responses when compared to IM vaccinates. For the purposes of the discussion, further comments regarding the different dose groups 15 combine the results of the IM and SC routes of administration. Regardless of the route of administration, an excellent p68 antibody response was demonstrated in both vaccinated groups by Day 50 and was maintained by vaccinates throughout the course of the study. A good anamnestic response was observed in vaccinates post challenge. 20 Comparison of the p68 ELISA titers of the 15 pg/dose and 60 pg/dose during the course of the study indicated that the 60 pg/dose group showed a slightly higher titer response (Figure 3). This response, although excellent, did not correlate with protection following aerosol challenge. p68 ELISA titer responses were variable in dogs removed from the study with positive tracheal swabs or rising agglutination titers. Three of the six controls removed 25 from the study with positive tracheal swab cultures and/or rising agglutination titers maintained p68 ELISA titers of <200. There appears to be no correlation of p68 ELISA titers to the tracheal swab or agglutination titer status of dogs removed from the study. Examination of the SAA response in vaccinated and unvaccinated p68 Bordetella dogs following challenge indicated a much smaller rise in the SAA values in the 15 pg/dose 30 groups especially on days 5 and 7 post-challenge. Conclusions In this study, efficacy a 15 pg/dose and 60 pg/dose of a p68 canine Bordetella vaccine was examined using a canine challenge model 6 months after vaccination. Both vaccines 35 were safe as demonstrated by normal rectal temperatures and minimal injection site reactions. Although both the 15pg/dose and the 6Og/dose vaccinated dogs showed good serological response to vaccination as measured by p68 ELISA titers, the response did not correlate with clinical protection in the 60 pg/dose vaccinated dogs. The 60g/dose vaccinated dogs demonstrated no significant difference in coughing when compared to unvaccinated 40 controls. Good efficacy of the 15pg/dose vaccine was demonstrated by a greater than 50% 52 reduction in coughing when compared to controls. It is postulated that increased levels of SDS in the 60 pg/dose vaccine may result in the demonstrated difference in protection. Comparison of SAA values demonstrated a difference between vaccinates and controls. EXAMPLE 4 5 Safety and Efficacy of VANGUARD@ Plus 5/CV-L VANGUARD@ Plus 5/CV-L is a freeze-dried preparation of attenuated strains of CD virus, CAV-2, CPI virus, CPV, and inactivated whole cultures of L. canicola and L. icterohaemorrhagiae, plus a liquid preparation of inactivated CCV with an adjuvant. All viruses 10 were propagated on established cell lines. The CPV fraction was attenuated by low passage on the canine cell line which gave it the immunogenic properties capable of overriding maternal antibody interference at the levels indicated in Table 38. The liquid component was used to rehydrate the freeze-dried component, which had been packaged with inert gas in place of vacuum. 15 Laboratory evaluation demonstrated that VANGUARD® Plus 5/CV-L immunized dogs against CD, ICH, CAV-2 and CPI respiratory disease, enteritis caused by CCV and CPV, and leptospirosis caused by L. canicola and L. icterohaemorrhagiae, and that no immunologic interference existed among the vaccine fractions. Extensive field safety trials showed it to be safe and essentially reaction-free in dogs as young as 6 weeks of age under normal usage 20 conditions. It was also demonstrated that CAV-2 vaccine cross-protects against ICH caused by CAV-1. Studies demonstrated that CAV-2 not only protects against ICH, but against CAV-2 respiratory disease as well. Canine adenovirus type 2 challenge virus was not recovered from CAV-2-vaccinated dogs in tests conducted. 25 The CPV fraction in VANGUARD@ Plus 5/CV-L was subjected to comprehensive safety and efficacy testing. It was shown to be safe and essentially reaction-free in laboratory tests and in clinical trials under field conditions. Product safety was further demonstrated by a backpassage study which included oral administration of multiple doses of the vaccine strain to susceptible dogs, all of whom remained normal. 30 Research demonstrated that 3 doses of the vaccine with increased CPV virus titer can overcome serum neutralization (SN) titers associated with matemal antibody. Serum neutralization titers as low as 1:4 were shown by others to interfere with active immunization using conventional modified live vaccines. A clinical trial was conducted with fifty 6-week-old puppies [25 vaccinates (SN titer range -256) and 25 nonvaccinated controls (SN titer range 4 35 1024)] (Table 37). The group of vaccinates received 3 doses, with vaccinations administered 3 weeks apart beginning at 6 weeks of age. After 1 vaccination, 13/25 puppies exhibited a 4 fold or greater increase in CPV SN titer (seroconversion) (Table 38). Twelve of these 13 puppies had maternal SN titers s1:16 at the time of the first vaccination with the remaining puppy having an SN titer of 1:64. Another 9 puppies with initial SN titers between 1:16 and 40 1:256 seroconverted after the second vaccination. Their maternal antibody SN titers had declined to s1:64 at the time of the second vaccination. Similarly, the last 3 vaccinates, with 53 initial SN titers of 1:128, seroconverted after the third vaccination, after their maternal antibody CPV titer dropped 51:64. Therefore, in this study, when 3 doses of vaccine were given beginning at 6 weeks of age, all 25 vaccinates, even those with the highest maternal antibody levels, became actively immunized (GM = 1:1176; range of SN titers 128-4096). All 50 dogs 5 were challenged 3 weeks after the third vaccination with a heterologous CPV challenge virus. Fourteen of 25 nonvaccinated control dogs died or showed illness severe enough to warrant euthanasia, while all 25 vaccinates remained essentially healthy. The high-titer, low-passage vaccine virus in VANGUARD@ Plus 5/CV-L was therefore highly immunogenic and capable of stimulating active immunity in the presence of maternal antibodies. 10 The efficacy of the CCV fraction of VANGUARD@ Plus 5/CV-L was demonstrated in an extensive vaccination challenge study. Sixteen 7- to 8-week-old puppies were vaccinated with VANGUARD@ Plus 5/CV-L (vaccinates) and 17 with Vanguard@ Plus 5/L (controls). All puppies received three 1-mL doses at 3-week intervals. Three weeks following the third vaccination, puppies were challenged with a virulent strain of CCV (CV-6). Clinical 15 observations, temperatures, weights, and blood parameters were monitored for 21 days following infection. CCV vaccinates demonstrated a reduction in the occurrence of diarrhea and amount of virulent CCV shed when compared to controls. At 21 days postchallenge, fluorescent antibody staining for virulent CCV of small intestinal sections demonstrated a significant reduction (P) in detectable CCV antigen between CCV vaccinates and controls 20 (Table 39). Table 37 Initial Serum Neutralization (SN) Titers of Vaccinates and Controls SN Titers # Vaccinates Included # Controls Included <1:2 3 10 1:4 4 13 1:8 1 3 1:1 6 14 1 1:32 2 5 16 3 11 1:128 6 3 1:256 2 3 1:512 09' 5 1:1024 .0 1 Table 38. Postvaccination Serum Neutralization (SN) Titers Geometric Mean (Range)r Groups N Prevaccination Postvaccination 2 All Vaccinated 25 1:24 1:108 1:605 1:1176 Dogs (<2-256) (8-1024) (8-4096) (128->4096) 1Responders Post 13 1:6 1:460 1:1745 1:1410 1st Vaccination (<2-64) 1(64-1024) (256-4096) (256-4096) Responders Post 9 1:87 1:20 1:376 1:1625 2nd Vaccination (16-256) (8-64) (256-1024) -(256-4096) Responders Post 3 1:128 1:32 1:25 1:203 3rd Vaccination (128) (16-64) (8-64) -(128-256) Nonvaccinated 25 1:64 1:9 1:3 1<1:2 jControi Dogs (4-1024) (<2-64) (<2-64) |(<-4) 54 aDogs were vaccinated at 6, 9, and 12 weeks of age. bPre-challenge SN titers Table 39. Fluorescent Antibody Staining of Small Intestinal Sections 21 Days Following Challenge 5 Gut Section % Dogs Fluorescent Antibody Positive Vaccinates Icontrols 1i0 89 Duodenum 2 0 100 3 0 00 Jejunum 4 10 89 5 0 100 6 112.5 56 Ileum 7 0 78 18 112.5 178 F 10 167 10 112.5 156 Conclusions In this study, an adjuvanted combination vaccine containing CD virus, CAV-2, CPI virus, CPV 10 and inactivated whole cultures of L. canicola and L. icterohaemorrhagiae and CCV, was shown to be both safe and efficacious as a vaccine when used in puppies. The combination vaccine was also shown to overcome serum neutralization (SN) titers associated with maternal antibody. EXAMPLE 5 15 CANINE BORDETELLA NATIVE p68 IMMUNOGENICITY STUDY Animals The study included two litters of SPF beagles and two litters of random source dogs. Dogs were assigned randomly to vaccinated or non-vaccinated groups. The study included a 20 total of 10 vaccinated and 11 non-vaccinated dogs. Preparation of Experimental Vaccine B. bronchispetica (strain 11 OH) was harvested froma 48 hour Bordet-Gengou blood agar spread plates by washing the plate surface with 5 to 10 ml heat extraction buffer. 25 Alternatively, cells grown in both culture (Charlotte Parker Defined Medium) were harvested by centrifugation discarding the supernatant fraction. Harvested cells were suspended in 25 mM Tris-HCL, pH 8.8 and incubated at 600C for 1 hour. Cell debris was separated from heat extract by centrifugation at 20,000 x g at 40C fro 30 minutes. Sodium azide (0.01%) was added to the heat extracted supernatant fraction which was then further clarified by 30 microporous filtration.
55 Monoclonal antibody affinity resin was prepared by conjugation of monoclonal antibody (designated Bord 2-7) to CNBr-activated Sepharose 4B using standard procedures. Approximately 30.35 mg of monoclonal antibody was conjugated to 1 gram of affinity resin. Clarified heat extracted supernatant fraction (above) and Bord 2-7 affinity resin was combined 5 at an approximate ratio of 1 liter extract to 20 ml resin. Binding of the native p68 to the resin was facilitated by incubating the mixture at ambient temperature, with gentle shaking, overnight, followed by resin settling and aspiration of the supernatant fraction. The resin was then packed into a 2.6 cm diameter column and the column washed sequentially with PBS, pH 7.5 and 10 mM phosphate buffer, pH 8.0 at a flow 10 rate of 5 ml/min. When absorbance at 280 nm reached a baseline level, bound material was eluted using 100 mM triethylamine and fractions under the single large peak of 280 nm absorbance were collected and tested for the presence of p68 by ELISA. Fractions containing p68 were pooled and dialyzed against PBS to remove triethylamine. An experimental vaccine serial formulated was formulated to contain approximately 15 100 micrograms of purified p68 and 1% aluminum hydroxide gel. Formalin (0.01%) was used as a preservative in a final vaccine dose volume of 1 mL. Challenge Inoculum Challenge material was prepared essentially as described in examples above. 20 Study Procedure Twenty-one (21) seronegative and culture negative pups were randomly assigned to one of two treatment groups. Eleven dogs were assigned to the non-vaccintated, control group and ten dogs to the vaccinated group. Day 0 was designated as the day of first 25 vaccination. One mL of vaccine was administered subcutaneously on Day 0 and repeated 21 days later. Blood was collected for serological p68 ELISA prior to first and second vaccination. On Day 35, fourteen days after the second vaccination, an aerosol challenge of B. bronchiseptica was administered to all dogs as described above. Animals were monitored for coughing for 14 days following challenge as described in previous examples. 30 Results Summary of clinical observations and serologic responses to p68 are presented in Table 40. Table 40: Summary of Clinical Observations and Serologic Responses 35 GROUP N NUMBER NORMAL/ TITER (GMT) TITER (GMT) TITER (GMT) COUGH ON TOTAL PRE-VAX PRE- POST TWO CHALLENGE CHALLENGE CONSECUT IVE DAYS VACCINE 10 0 10/10 5.08 348.15 391.30 . CONTROL 11 11 0/11 6.10 6.45 18.81 56 Discussion In this study, 10 of 10 control dogs coughed on at least two consecutive days. A dog is considered clinically diseased if it coughs for two consecutive days. By this criteria, 100% of the non-vaccinated control dogs were diseased. In the vaccinated group, one dog coughed 5 on day 4 post-challenge and one dog coughed on days 4 and 6 post-challenge. Two dogs coughed on day 14. None of the vaccinated dogs coughed for two consecutive days. Therefore, 100% of the dogs in the native p68 vaccinated group were judged to remain normal following challenge. 10 Conclusions This trial demonstrates the ability of a native p68 vaccine to protect against B. bronchiseptica disease. EXAMPLE 6 15 Efficacy of multivalent canine vaccines against Leptospira bratislava challenge The purpose of this study was to demonstrate the efficacy of vaccines containing fractions to Leptospira serovars bratislava, canicola, grippotyphosa, icterohaemorrhagiae and pomona against L. bratislava challenge in dogs. MATERIALS 20 Vaccines: Vaccines used were the following: 1. A lyophilized vaccine comprising canine distemper, adenovirus type 2, parainfluenza, and parvovirus antigens (VANGUARD @ PLUS 5) was used. The vaccine contained release antigen levels. Product code 13D1.22 2. A canine coronavirus vaccine in a liquid diluent formulation (FIRSTDOSE@ CV) 25 was used. The vaccine contained release antigen levels. Product code 14P5.20 3. A lyophilized vaccine comprising canine distemper, adenovirus type 2, parainfluenza, parvovirus, bratislava, canicola, grippotyphosa, icterohaemorrhagiae and pomona antigens was used. The vaccine contained approximately 600 nephlos of each Leptospira serovar; and release antigen levels of the modified live virus fractions (canine 30 adenovirus, distemper virus, parainfluenza virus, parvovirus). Product code 4637.2A Study Animals: Beagle puppies of approximately 5-6 weeks of age of either sex were used. They were seronegative (< 1:8) to Leptospira serovars bratislava, canicola, grippotyphosa, icterohaemorrhagiae and Pomona at vaccination. They were allowed commercial feed and city-system sourced water ad libitum. 35 Challenge Organism: Each animal was administered one dose of approximately 2 mL (101 dilution of infected hamster liver tissue) of Leptospira bratislava as an intraperitoneal injection.
57 Study Design Trt. Vaccine No. Vacc. Leptospira Challenge** Blood Sample Route Cleto No. Descrip.* Dogs Day Day Dose Dilution Collection Days T1 Control SQ 10 0 & 21 49 2 mL 10-1 0, 21, 35, 48, 50,52, 55, 58, 61,64,67, T2 Control IM 10 0 & 21 49 2 mL 10-1 0, 21, 35, 48, 50, 52, 55, 58, f__ 1__ _ _ _ ____ _ _ ___ 61, 64, 67, 70 T3 Leptospira SQ 10 0 & 21 49 2 mL 10-1 0, 21, 35, 48, 50,52,55,58, 61, 64, 67, 70 T4 Leptospira IM 10 0 & 21 49 2 mL 10-1 0, 21, 35, 48, 50, 52, 55, 58, 61, 64, 67, 70 PROCEDURES Vaccination Phase: On Study Days 0 and 21, 40 dogs in 4 vaccinate groups (10 5 animals/group) were given an injection of the control or test vaccines as outlined below: T1: 1 mL of control vaccine containing canine distemper, adenovirus type 2, parainfluenza, parvovirus vaccine reconstituted with canine coronavirus diluent and given by subcutaneous (SQ) injection. (Product Code 13D1.22 reconstituted with Product Code 14P5.20) 10 T2: 1 mL of control vaccine containing canine distemper, adenovirus type 2, parainfluenza, parvovirus vaccine reconstituted with canine coronavirus diluent and given by intramuscular (IM) injection. (Product Code 13D1.22 reconstituted with Product Code 14P5.20) T3: I mL of Leptospira vaccine containing canine distemper, adenovirus type 2, 15 parainfluenza, parvovirus, bratislava, canicola, grippotyphosa, icterohaemorrhagiae and pomona vaccine reconstituted with canine coronavirus diluent and given by SQ injection. (Product Code 46J7.2A; a combination of Product Codes 4637.2A and 14P5.20) T4: 1 mL of Leptospira vaccine containing canine distemper, adenovirus type 2, parainfluenza, parvovirus, bratislava, canicola, grippotyphosa, icterohaemorrhagiae and 20 pomona vaccine reconstituted with canine coronavirus diluent and given by IM injection. (Product Code 46J7.2A; a combination of Product Codes 4637.2A and 14P5.20) Post-vaccination observations for untoward systemic reactions were made at approximately 1 hour and 5 hours following vaccination. Leptospira Challenge: On Study Days 49, the test animals were challenged via an 25 approximate 2 mL dose of L. bratislava by intraperitoneal injection.
58 Blood Sample Collection: Serum samples were collected from available animals on Study Days 0, 21, 35, 48, 50, 52, 55, 58, 61, 64, 67 and 70. Similarly, plasma samples were collected on Study Days 48, 50, 52, 55, 58, 61, 64, 67 and 70. Bacterial Serology: Serum samples obtained on Study Days 0, 21, 35, 48, 58 and 5 70 were assayed via a microagglutination test for circulating antibodies to L. bratislava. Spirochetemia: Plasma samples obtained on Study Days 48, 50, 52, 55, 58, 61, 64, 67 and 70 were examined by dark-field microscopy for spirochetes and cultured for Leptospira re-isolation. Complete Blood Count / Serum Chemistry Panel: Plasma samples obtained on 10 Study Days 48, 50, 52, 55, 58, 61, 64, 67 and 70 were assayed for, but not limited to, platelet counts and sedimentation rate. Serum samples, obtained at those same intervals were assayed for, but not limited to, amylase, alanine aminotransferase (ALT), aspartate aminotransaminase (AST) and creatinine. A CBC and sedimentation rate. was not completed for 2 animals (Nos. QPH3, RVG3) on Study Day 55, for one animal (No. RBH3) on Study Day 15 58, for 3 animals (Nos. OLH3, OUH3, PTG3) on Study Day 61, nor for one animal (No. OWG3) on Study Day 70 because the plasma sample collected clotted prior to testing. On Study Day 67, a sedimentation rate was not completed for 2 animals (Nos. OUH3, SAG3) because the plasma sample quantity was insufficient for testing. Those outcomes had no impact on these study parameters. 20 Rectal Body Temperatures: Rectal body temperatures were recorded on Study Days 47-70. Post-challenge (Study Day 50), an elevated body temperature of 2 39.2 *C was considered indicative of leptospirosis. Urine Cultures: Urine samples obtained on Study Days 48, 55 and 70 were cultured for Leptospira, and submitted for urinalysis. On Study Day 48, a urinalysis was not completed 25 for one animal (No. CBC3) as the quantity of urine available at collection was insufficient for testing. On Study Day 55, a urinalysis was not completed for 2 animals (No. OPH3, RXG3) as the quantity of urine available at collection was insufficient for testing. On Study Day 70, a urinalysis was not completed for 10 animals (Nos. OYG3, PIG3, PUG3, QHG3, QQH3, QSG3, RDG3, RUG3, RVG3, RYG3) as the quantities of urine available at collection were insufficient 30 for testing. Those outcomes had no impact on this study parameter. Necropsy and Leptospira Isolation: Animals euthanized during or at conclusion of the post-challenge period were necropsied. Body fluids and tissues (i.e., liver, kidney and urine) were collected and submitted to BCL for Leptospira re-isolation. On Study Day 55, bacterial re-isolation was not completed for 2 animals (No. OPH3, RXG3) as the quantity of 35 urine available at collection was insufficient for testing. On Study Day 70, bacterial re-isolation was not completed for 4 animals (Nos. OYG3, PUG3, QSG3, RUG3) as the quantities of urine available at collection were insufficient for testing. Those outcomes had no impact on this study parameter.
59 Health Observations: Animals were monitored daily for general health status. DATA SUMMARY AND ANALYSIS Data were analyzed with a mixed model or categorical (SAS/STAT Software Changes 5 and Enhancements through Release 6.12, SAS Institute, Cary, North Carolina) procedure. A general linear repeated measures mixed model (fixed effect model terms are treatment, study day, and treatment by study day) was used to analyze temperature, serum antibody titers, blood platelet count, sedimentation rate, amylase, alanine aminotransferase (ALT), aspartate aminotransaminase (AST) and creatinine. Contrasts of interest were made 10 after detecting a significant (P s0.05) treatment or treatment by day of study interaction effect. Titers were log-transformed as appropriate for analysis, and when transformed, the least squares means were back-transformed to geometric means for presentation. Observations not analyzed (i.e., necropsy results and post-vaccination observations) were not entered into the database for summary. 15 Frequency distributions of animals with platelet counts < 200 at least once and animals with rectal temperatures greater than or equal to 39.2 *C were calculated for each treatment. Animals were classified as normal or ill on each day post-challenge during the study. The presence of any of the following signs resulted in a classification of ill: conjunctivitis, 20 depression, inappetence, muscle tremors, nasal discharge, pyrexia (2 39.2 *C) or watery eyes. A general linear mixed model (fixed effect model term is treatment) was used to analyze the number of days post-challenge that an animal was classified as ill. The binomial variable died or euthanized was analyzed with Fisher's Exact test. Spirochetemia, and 25 bacteria re-isolation in the urine, kidney and liver were analyzed using Fisher's Exact test to compare treatment groups. In the absence of a significant difference between the routes of administration and route by treatment interaction (P50.05 two-sided), contrasts were used to compare the average of treatments T1 and T2 to the average of treatments T3 and T4 (P50.05 one-sided). 30 If the analysis was a repeated measures analysis, then the comparison was made at each time point data was collected. Otherwise, contrasts were used to compare Ti to T3 and T2 to T4 (Ps0.05 one-sided). These comparisons were made at each time point if the analysis was a repeated measures. The efficacy of the Leptospira vaccine against L. bratislava was demonstrated by a 35 lower incidence (P 0.05, one sided) of illness in the vaccinated animals. The following variables supported the efficacy of the Leptospira vaccine: (1) The mean platelet count was significantly (P s 0.05, one sided) higher for the vaccinates; (2) The mean sedimentation rate was significantly (P 5 0.05; one sided) lower for the vaccinates; (3) The incidence of spirochetemia was significantly (P 5 0.05, one sided) reduced for the vaccinates. 40 60 RESULTS Clinical Signs Post-Challenge: The mean number of days that the test animals displayed clinical signs indicative of leptospirosis (e.g., conjunctivitis, depression, diarrhea, hematuria, icterus, inappetence, moribund, muscle tremors, pyrexia, vomiting) is presented in 5 Table 1. Post-challenge (Study Days 50-70), the mean number of days the T1 and T2 controls were ill was 4.3 and 3.3, respectively. Conversely, the means for the T3 and T4 vaccinates were 0.7 and 0.5, and those results were significantly improved (P<0.05) when compared to the T1-T2 controls. The percent of animals presenting with clinical signs post-challenge is also provided in Table 1. Signs of Leptospirosis was displayed by 75% of the T1 -T2 controls, while similar signs 10 were observed for only 30% of the T3-T4 vaccinates. The number of infected animals requiring euthanasia during the challenge phase of the study is also presented in Table 1. Five animals (25%) in the T1-T2 control groups displayed severe signs of leptospirosis and were euthanized. Conversely, the T3-T4 vaccinates remained otherwise healthy during that same interval, and that comparison (TI -T2 vs T3-T4) was significantly improved (P<0.05) for the vaccinates. A 15 representation of mean body temperatures post-challenge is presented in Table 2. During the initial 9 days after challenge (Study Days 50-58), the mean temperatures for the T1 -T2 controls ranged from 37.8 to 39.40C. During that same interval, the mean temperatures for the T3-T4 vaccinates ranged from 38.2 to 38.7 *C. Notably, the mean temperatures for the T3-T4 vaccinates were significantly lower (P<0.05) at 2, 3 and 5 days post-challenge when compared to 20 the T1-T2 mean temperatures. The frequency of animals with at least one temperature 2 39.2 0C is also presented in Table 2. An elevated body temperature of 39.2 0C was considered indicative of Leptospira infection. During the course of the post-challenge observation period, 60 70% of the T1 -T2 controls presented with a temperature of 2 39.2 *C. Conversely, only 30% of the T3-T4 vaccinates presented with that clinical sign. 25 Spirochetemia Post-Challenge: The frequency of spirochetemia is presented in Table 3. The presence of spirochetes in the blood (detected via bacterial culture) is a clinical outcome demonstrating leptospirosis. One day post-challenge (Study Day 50), spirochetemia was established in 90% of the TI controls, 60% of the T2 controls, 60% of the T3 vaccinates and 70% of the T4 vaccinates. Bacterial re-isolation was expected from the blood at 24 hours 30 after intraperitoneal injections regardless of the status of vaccination. Thereafter, spirochetemia was established for the T1 controls as follows: 100% on Day 3 post-challenge (Study Day 52), 56% on Day 6 (Study Day 55) and 33% of Day 9 (Study Day 58). Similarly, spirochetemia was established for the T2 controls as follows: 60% on Day 3 post-challenge, 50% on Day 6 and 29% of Day 9. Conversely, it was not established in the T3-T4 vaccinates 35 during that same post-challenge period nor during the remainder of the post-challenge period (i.e., Study Days 50-70). Overall, the percentage of animals that were positive for spirochetemia including one day post-challenge (Study Days 50-70) was 60% - 100% for the T1 -T2 controls and 60%-70% for the T3-T4 vaccinates. In contrast, the percentage of animals that were positive excluding 40 one day post-challenge (Study Days 52-70) was 60% - 100% for the T1 -T2 controls and 0% for the T3-T4 vaccinates.
61 Leptospira Re-Isolation from Body Fluids and Tissues Post-Challenge: Leptospira re-isolation from blood, urine, kidney and liver samples is presented in Table 4. A summary of Leptospira re-isolation results from blood is provided in the preceding section. Beginning at 3 days post-challenge (i.e., excluding day 1 post-challenge), spirochetemia was 5 established in 60-100% of the T1-T2 controls, and was not established in the T3-T4 vaccinates during that same period. Notably, that comparison (T1 -T2 vs T3-T4) was significantly improved (P<0.05) for the vaccinates. Kidney samples were collected at necropsy. Leptospira was re-isolated from 50% of the kidney samples obtained from the T1 and T2 controls. It was not re-isolated from samples 10 derived from the T3-T4 vaccinates. That comparison (T1 -T2 vs T3-T4) was significantly improved (P<0.05) for the vaccinates. Liver samples were collected at necropsy. Leptospira was re-isolated from 10% and 20% of the liver samples obtained from the T1 and T2 controls, respectively. It was not re isolated from samples derived from the T3-T4 vaccinates. That comparison (T1-T2 vs T3-T4) 15 was significantly improved (P<0.05) for the vaccinates. Urine samples were collected at 2 intervals post-challenge and at necropsy. Leptospira was re-isolated from 2 T1 controls at 6 days post-challenge (Study Day 55). Leptospira was not re-isolated from any sample for the T2 controls nor T3-T4 vaccinates. Platelet Counts Post-Challenge: Mean platelet counts are presented in Table 5. A 20 decrease in the number of blood platelets (thrombocytopenia) is a clinical result indicative of leptospirosis. Mean concentrations for the T1 -T2 controls ranged between 61 and 937. During the same interval, the mean counts for the T3-T4 vaccinates ranged between 400 and 566. Notably, the platelet counts for the T3-T4 vaccinates were significantly improved (P<0.05) at 3, 6 and 9 days post-challenge when compared to the T1-T2 controls. 25 The frequency of animals with at least one platelet count < 200 is also presented in Table 5. During the course of the post-challenge period, 90% of the T1 controls and 60% of the T2 controls were determined to be thrombocytopenic (platelet count < 200). Conversely, only 10% of the T3 vaccinates and none of the T4 vaccinates were thrombocytopenic post-infection. Sedimentation Rates Post-Challenge: Mean sedimentation rates are presented in Table 6. An 30 increase in sedimentation rate is a clinical result indicative of leptospirosis. Mean rates for the T1 -T2 controls ranged between 2.4 and 16.0. During the same interval, the mean rates for the T3-T4 vaccinates ranged between 1.5 and 6.3. Notably, the rates for the T3-T4 vaccinates were significantly lower (P<0.05) at 3, 6, 9, 12, 15, 18 and 21 days post-challenge when compared to the T1 -T2 controls. 35 ALT Concentrations Post-Challenge: Mean alanine aminotransferase (ALT) concentrations are presented in Table 7. An increase in ALT is a clinical result indicative of bacterial infection (i.e., as liver function deteriorates, ALT levels rise). Mean concentrations for the T1 -T2 controls ranged between 22 and 79. During the same interval, the mean concentrations for the T3-T4 vaccinates ranged between 21 and 61. Notably, the 40 concentrations for the T3-T4 vaccinates were significantly lower (P<0.05) at 3, 6, 9 and 12 days post-challenge when compared to the T1-T2 controls.
62 Creatinine Concentrations Post-Challenge: Mean creatinine concentrations are presented in Table 8. An increase in creatinine concentrations is a clinical result indicative of bacterial infection (i.e., as kidney function deteriorates due to leptospirosis, creatinine levels 5 rise). Mean concentrations for the T1 controls (i.e., SQ administration) ranged between 0.30 and .99. During the same interval, the mean concentrations for the T3 vaccinates (i.e., SQ administration) ranged between 0.26 and 0.40. Notably, the concentrations for the T3 vaccinates were significantly lower (P<0.05) at 1, 6, 9, 12, 15, 18 and 21 days post-challenge when compared to the T1 controls. Mean concentrations for the T2 controls (i.e., IM 10 administration) ranged between 0.30 and 1.31. During the same interval, the mean concentrations for the T4 vaccinates (i.e., IM administration) ranged between 0.32 and 0.46. There were no significant differences between the T2 and T4 values post-challenge. Amylase, AST and Urinalysis Post-Challenge: There were no significant 15 differences in the mean concentrations for the T3-T4 vaccinates when compared to the T1 -T2 controls. However, in general the post-challenge concentrations were more dramatically increased for the T1 -T2 controls. By and large, post-challenge changes in AST (aspartate aminotransaminase) and urinalysis results were not observed for the T1- T4 test animals. The results for these 3 parameters are not otherwise tabulated herein. 20 Serum Antibody Titers: Mean serum L. bratislava antibody titers are presented in Table 9. During the vaccination phase of the investigation (Study Days 0-48), the mean titers for the T1-T2 controls were approximately 2 (i.e., seronegative). Correspondingly, the means for the T3-T4 vaccinates ranged between 2 (pre-vaccination) and 1181 (post-vaccination). Notably, the mean titers for the T3-T4 vaccinates were significantly higher (P<0.05) at 21, 35 25 and 48 days post-vaccination when compared to the mean T1 - T2 titers. During the challenge phase of the investigation (Study Days 58 & 70), the mean titers for the T1-T2 controls ranged between 2135 and 41160. Correspondingly, the mean titers for the T3 and T4 vaccinates ranged between 727 and 10891, and were significantly lower (P<0.05) on Study Days 58 (i.e., 9 days post-challenge) and 70 days post-vaccination (i.e., 30 Day 21 PC) when compared to the T1 -T2 controls. As a result, the controls demonstrated a dramatic primary response to L. bratislava post-challenge (i.e., infection established), while the response for the T3-T4 vaccinates was less robust (i.e., typical anamnestic response). Systemic Reactions Post-Vaccination: No post-vaccinal systemic reactions were observed in the T1 -T4 test animals when evaluated at approximately 1 and 5 hours after the 35 primary and booster vaccinations. That result is not tabulated herein. CONCLUSION The efficacy of a multivalent Leptospira vaccine given by SQ or IM injection against L. bratislava was demonstrated post-challenge by, inter alia: (1) a significantly lower incidence of 40 Leptospira-associated illness, (2) a significantly lower incidence of spirochetemia, (3) 63 significantly higher platelet counts, and (4) significantly lower mean sedimentation rates, when compared to controls.
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Claims (16)
1. A combination vaccine for immunizing dogs against canine pathogens comprising a Leptospira cell preparation of Leptospira canicola, Leptospira grippotyphosa, Leptospira icterohaemorrhagiae, Leptospira bratislava and Leptospira pomona, and further comprising an 5 attenuated strain of canine distemper (CD) virus, an attenuated strain of canine adenovirus type 2 (CAV-2), an attenuated strain of canine parainfluenza (CPI) virus, an attenuated strain of canine parvovirus (CPV), and a carrier.
2. The combination vaccine of claim 1, wherein the amount of each Leptospira strain in the vaccine is in the range of about 100-3500 nephelometric units per vaccine dose. 10
3. The combination vaccine of claim 1, wherein the amount of said attenuated strain of CD virus, CAV-2, CPI, and CPV in said vaccine is in the range of 102 to 109 TCIDSO per dose for each virus.
4. The combination vaccine of any one of claims 1 to 3, wherein said carrier comprises saponin and a surfactant. 15
5. The combination vaccine of claim 4, wherein said saponin is Quil A and said surfactant is cholesterol.
6. The combination vaccine of any one of claims 1 to 5, wherein the carrier comprises aluminium hydroxide.
7. The combination vaccine of claim 1, further comprising an inactivated whole or 20 partial cell preparation of a strain of canine coronavirus (CCV).
8. The combination vaccine of claim 7, wherein the amount of each Leptospira strain in the vaccine is in the range of about 100-3500 nephelometric units per vaccine dose.
9. The combination vaccine of claim 7, wherein the amount of said attenuated strain of CD virus, CAV-2, CPI, and CPV in said vaccine is in the range of 102 to 109 TCID 50 per dose for 25 each virus.
10. The combination vaccine of claim 7, wherein the amount of the cell preparation of said strain of CCV in said vaccine is at least about 100 relative units per dose .
11. The combination vaccine of any one of claims 7 to 10, wherein said carrier comprises saponin and a surfactant. 30
12. The combination vaccine of claim 11, wherein said saponin is Quil A and said surfactant is cholesterol.
13. The vaccine composition of any one of claims 7 to 12 wherein the carrier comprises aluminium hydroxide.
14. A combination vaccine for immunizing dogs against canine pathogens comprising a 35 Leptospira cell preparation of Leptospira canicola, Leptospira grippotyphosa, Leptospira 74 icterohaemorrhagiae, Leptospira bratislava and Leptospira pomona, and further comprising an attenuated strain of canine distemper (CD) virus, an attenuated strain of canine adenovirus type 2 (CAV-2), an attenuated strain of canine parainfluenza (CPI) virus, an attenuated strain of canine parvovirus (CPV), substantially as hereinbefore described with reference to Example 6. 5
15. A method for immunizing dogs against canine pathogens comprising administering to a dog the combination vaccine of any one of claims 1 to 14.
16. Use of the combination vaccine of any one of claims 1 to 14 in the manufacture of a vaccine for immunizing dogs against canine pathogens.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2012244091A AU2012244091A1 (en) | 2003-01-29 | 2012-10-19 | Multivalent canine vaccines against leptospira bratislava and other pathogens |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US60/443,418 | 2003-01-29 | ||
| AU2005290921A AU2005290921C1 (en) | 2003-01-29 | 2005-09-23 | Multivalent canine vaccines against Leptospira bratislava and other pathogens |
| AU2012244091A AU2012244091A1 (en) | 2003-01-29 | 2012-10-19 | Multivalent canine vaccines against leptospira bratislava and other pathogens |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2005290921A Division AU2005290921C1 (en) | 2003-01-29 | 2005-09-23 | Multivalent canine vaccines against Leptospira bratislava and other pathogens |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2012244091A1 true AU2012244091A1 (en) | 2012-11-15 |
Family
ID=47144651
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012244091A Abandoned AU2012244091A1 (en) | 2003-01-29 | 2012-10-19 | Multivalent canine vaccines against leptospira bratislava and other pathogens |
Country Status (1)
| Country | Link |
|---|---|
| AU (1) | AU2012244091A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CZ307883B6 (en) * | 2013-01-29 | 2019-07-24 | Bioveta, A.S. | Multivalent vaccine for immunoprophylaxis of infectious diseases in dogs |
-
2012
- 2012-10-19 AU AU2012244091A patent/AU2012244091A1/en not_active Abandoned
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CZ307883B6 (en) * | 2013-01-29 | 2019-07-24 | Bioveta, A.S. | Multivalent vaccine for immunoprophylaxis of infectious diseases in dogs |
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Free format text: IN VOL 26 , NO 43 , PAGE(S) 5546 UNDER THE HEADING COMPLETE APPLICATIONS FILED - NAME INDEX UNDER THE NAME PFIZER PRODUCTS INC, APPLICATION NO. 2012244091, UNDER INID (54) CORRECT THE TITLE TO READ MULTIVALENT CANINE VACCINES AGAINST LEPTOSPIRA BRATISLAVA AND OTHER PATHOGENS |
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| DA3 | Amendments made section 104 |
Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE INVENTION TITLE TO READ MULTIVALENT CANINE VACCINES AGAINST LEPTOSPIRA BRATISLAVA AND OTHER PATHOGENS |
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