AU2012216406A1 - Modification of feeding behavior - Google Patents

Abstract

Methods are disclosed for decreasing calorie intake, food intake, and appetite in a subject. The methods include peripherally administering PYY or an agonist thereof and GLP-1 or an agonist thereof to the subject, simultaneously or sequentially, thereby decreasing the calorie intake of the subject. SIS S/G Ex/G Fig. 11 Fig. 12 SUBSTITUTE SHEET (RULE 26)

Description

AUSTRALIA Patents Act 1990 OREGON HEALTH AND SCIENCE UNIVERSITY, IMPERIAL INNOVATIONS LIMITED COMPLETE SPECIFICATION STANDARD PATENT Invention Title: Modification offeeding behavior The following statement is a full description of this invention including the best method of performing it known to us:- -1A MODIFICATION OF FEEDING BEIHA VIOUR PRIORITY CLAIM This application claims the benefit of UK Application No. GB0200507.2 5 filed January 10, 2002, and International Application PCT/ US02/31944 which are both incorporated by reference in their entirety herein. STATEMENT OF GOVERNMENT SUPPORT This disclosure was made in part with United States government support 10 pursuant to grants RR00163, DK51730 and DK55819, from the National Institutes of Health. The United States government has certain rights in the disclosure. FIELD This application relates to the use of agents to control appetite, feeding, food 15 intake, energy expenditure and calorie intake, particularly in the field of obesity. BACKGROUND According to the National Health and Nutrition Examination Survey (NHANES III, 1988 to 1994), between one third and one half of men and women in 20 the United States are overweight. In the United States, sixty percent of men and fifty-one percent of women, of the age of 20 or older, are either overweight or obese. In addition, a large percentage of children in the United States are overweight or obese. The cause of obesity is complex and multi-factorial. Increasing evidence 25 suggests that obesity is not a simple problem of self-control but is a complex disorder involving appetite regulation and energy metabolism. In addition, obesity is associated with a variety of conditions associated with increased morbidity and mortality in a population. Although the etiology of obesity is not definitively established, genetic, metabolic, biochemical, cultural and psychosocial factors are 30 believed to contribute. In general, obesity has been described as a condition in which excess body fat puts an individual at a health risk. SUBSTITUTE SHEET (RULE 26) -2 There is strong evidence that obesity is associated with increased morbidity and mortality. Disease risk, such as cardiovascular disease risk and type 2 diabetes disease risk, increases independently with increased body mass index (BMI). Indeed, this risk has been quantified as a five percent increase in the risk of cardiac 5 disease for females, and a seven percent increase in the risk of cardiac disease for males, for each point of a BMI greater than 24.9 (see Kenchaiah et al., N. Engl. J. Med. 347:305, 2002; Massie, N. Engl. J' Med. 347:358, 2002). In addition, there is substantial evidence that weight loss in obese persons reduces important disease risk factors. Even a small weight loss, such as 10% of the initial body weight in both 10 overweight and obese adults has been associated with a decrease in risk factors such as hypertension, hyperlipidemia, and hyperglycemia. Although diet and exercise provide a simple process to decrease weight gain, overweight and obese individuals often cannot sufficiently control these factors to effectively lose weight. Pharmacotherapy is available; several weight loss drugs 15 have been approved by the Food and Drug Administration that can be used as part of a comprehensive weight loss program. However, many of these drugs have serious adverse side effects. When less invasive methods have failed, and the patient is at high risk for obesity related morbidity or mortality, weight loss surgery is an option in carefully selected patients with clinically severe obesity. However, these 20 treatments are high-risk, and suitable for use in only a limited number of patients. It is not only obese subjects who wish to lose weight. People with weight within the recommended range, for example, in the upper part of the recommended range, may wish to reduce their weight, to bring it closer to the ideal weight. Thus, a need remains for agents that can be used to effect weight loss in overweight and obese 25 subjects. SUMMARY Disclosed herein are findings that peripheral administration of PYY, or an agonist thereof, to a subject results in decreased food intake, caloric intake, and 30 appetite, and an alteration in energy metabolism. The subject can be any subject, including, but not limited to, a human subject. In several embodiments, the subject -1 MODIFICA ION OF FEEDING BEH VIOUR PRIORITY CLAIM This application claims the benefit of UK Application No. GB0200507.2 5 filed January 10, 2002, and International Application PCT/ US02/31944 which are both incorporated by reference in their entirety herein. STATEMENT OF GOVERNMENT SUPPORT This disclosure was made in part with United States government support 10 pursuant to grants RROO163, DK51730 and DK55819, from the National Institutes of Health. The United States government has certain rights in the disclosure. FIELD This application relates to the use of agents to control appetite, feeding, food 15 intake, energy expenditure and calorie intake, particularly in the field of obesity. BACKGROUND According to the National Health and Nutrition Examination Survey (NHANES III, 1988 to 1994), between onc third and one half of men and women in 20 the United States are overweight. In the United States, sixty percent of men and fifty-one percent of women, of the age of 20 or older, are either overweight or obese. In addition, a large percentage of children in the United States are overweight or obese. The cause of obesity is complex and multi-factorial. Increasing evidence 25 suggests that obesity is not a simple problem of self-control but is a complex disorder involving appetite regulation and energy metabolism. In addition, obesity is associated with a variety of conditions associated with increased morbidity and mortality in a population. Although the etiology of obesity is not definitively established, genetic, metabolic, biochemical, cultural and psychosocial factors are 30 believed to contribute. In general, obesity has been described as a condition in which excess body fat puts an individual at a health risk. SUBSTITUTE SHEET (RULE 26) -2 There is strong evidence that obesity is associated with increased morbidity and mortality. Disease risk, such as cardiovascular disease risk and type 2 diabetes disease risk, increases independently with increased body mass index (BMI). Indeed, this risk has been quantified as a five percent increase in the risk of cardiac 5 disease for females, and a seven percent increase in the risk of cardiac disease for males, for each point of a BMI greater than 24.9 (see Kenchaiah et al., N. Engl. J. Med. 347:305, 2002; Massie, N. Engl. .J Med. 347:358, 2002). In addition, there is substantial evidence that weight loss in obese persons reduces important disease risk factors. Even a small weight loss, such as 10% of the initial body weight in both 10 overweight and obese adults has been associated with a decrease in risk factors such as hypertension, hyperlipidemia, and hyperglycemia. Although diet and exercise provide a simple process to decrease weight gain, overweight and obese individuals often cannot sufficiently control these factors to effectively lose weight. Pharmacotherapy is available; several weight loss drugs 15 have been approved by the Food and Drug Administration that can be used as part of a comprehensive weight loss program. However, many of these drugs have serious adverse side effects. When less invasive methods have failed, and the patient is at high risk for obesity related morbidity or mortality, weight loss surgery is an option in carefully selected patients with clinically severe obesity. However, these 20 treatments are high-risk, and suitable for use in only a limited number of patients. It is not only obese subjects who wish to lose weight. People with weight within the recommended range, for example, in the upper part of the recommended range, may wish to reduce their weight, to bring it closer to the ideal weight. Thus, a need remains for agents that can be used to effect weight loss in overweight and obese 25 subjects. SUMMARY Disclosed herein are findings that peripheral administration of PYY, or an agonist thereof, to a subject results in decreased food intake, caloric intake, and 30 appetite, and an alteration in energy metabolism. The subject can be any subject, including, but not limited to, a human subject. In several embodiments, the subject -3 desires to lose weight, is obese, overweight, or suffers from a weight-related disorder. PYY 3

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36 can preferably be administered to the subject. Also disclosed are findings that co-administration of PYY, or an agonist thereof, for example, PYY 3-36, with GLP-1 or an agonist thereof to a subject results 5 in a greater decrease in food intake, caloric intake, and appetite, than when PYY or an agonist thereof is administered alone. The effect is synergistic. In one embodiment, a method is disclosed for decreasing calorie intake in a subject. The method includes peripherally administering a therapeutically effective amount of PYY or an agonist thereof and GLP-1 or an agonist thereof to the subject, 10 thereby decreasing the calorie intake of the subject. In another embodiment, a method is disclosed for decreasing appetite in a subject. The method includes peripherally administering a therapeutically effective amount of PYY or an agonist thereof and GLP-1 or an agonist thereof to the subject, thereby decreasing the appetite of the subject. 15 In a further embodiment, a method is disclosed for decreasing food intake in a subject. The method includes peripherally administering a therapeutically effective amount of PYY or an agonist thereof, and GLP-1 or an agonist thereof to the subject, thereby decreasing the food intake of the subject. In yet another embodiment, a method is disclosed herein for increasing 20 energy expenditure in a subject. The method includes peripherally administering a therapeutically effective amount of PYY or an agonist thereof, and GLP-1 or an agonist thereof to the subject, thereby increasing energy expenditure in the subject. A method is also disclosed for decreasing calorie intake, food intake, or appetite in a human subject. The method includes peripherally injecting a 25 therapeutically effective amount of PYY or an agonist thereof in a pharmaceutically acceptable carrier to the subject in a pulse dose, thereby decreasing the calorie intake, food intake, or appetite of the subject, and also administering GLP-1 or an agonist thereof. In each method above, the GLP-1 or an agonist thereof may be administered 30 simultaneously or substantially simultaneously as the PYY or agonist thereof, or sequentially, in any order. The PYY or agonist thereof and the GLP-1 or agonist thereof may be administered in a single pharmaceutical composition or in separate -4 compositions, and they may be administered by the same route or my different routes. The foregoing and other features and advantages will become more apparent from the following detailed description of several embodiments, which proceeds 5 with reference to the accompanying figures. BRIEF DESCRIPTION OF THE FIGURES 10 Fig. 1 is a set of diagrams and digital images showing the generation of transgenic mice expressing EGFP in ARC POMC neurons. Fig. la is a schematic diagram of the structure of the POMC-EGFP transgene. Fig. lb is a digital image showing the identification of a single POMC neuron (arrowhead on recording electrode tip) by BGFP fluorescence (upper) and IR-DIC microscopy (lower) in a 15 living ARC slice prior to electrophysiological recordings. Fig. 1 c is a set of digital images showing the co-localization (bright, on right) of EGFP (left) and B-endorphin immunoreactivity (middle) in ARC POMC neurons. Scale bars: b & c, 50 pm. Fig. Id is a set of diagrams showing the distribution of EGFP-positive neuronal soma throughout the ARC nucleus. o = 5 cells, .= 10 cells. 20 Fig. 2 is a tracing and graphs showing activation of MOP-Rs hyperpolarizes the EGFP-labeled POMC neurons by opening G protein-coupled inwardly-rectifying potassium channels. Fig. 2a is a tracing showing met-enkephalin hyperpolarizes POMC neurons and inhibits all action potentials. The horizontal bar indicates the 25 time when 30 pM Met-Enk was bath-applied to the slice. Fig. 2b is a graph showing met-enkephalin current and reversal potential is shifted by extracellular K* concentration. Fig. 2c is a graph showing met-enkephalin activates MOP-Rs on POMC neurons. A Met-Enk (30 pM) current was observed and the MOP-R specific antagonist CTAP (1 pM) was applied for 1 minute. Following CTAP Met-Enk 30 elicited no current. The figure is representative of three experiments.

-5 Fig. 3 are tracings and graphs demonstrating that leptin depolarizes POMC neurons via a non-specific cation channel, and decreases GABAergic tone onto POM cells. Fig. 3a is a tracing demonstrating that leptin depolarizes POMG neurons and increases the frequency of action potentials within I to 10 minutes of 5 addition. The figure is a representative example of recordings made from 77 POMC neurons. Fig. 3b is a graph showing that leptin causes a concentration dependent depolarization of POMC cells. The depolarization caused by leptin was determined at 0.1, 1, 10, 50, and 100 nM (ECso =5.9 nM) in (8, 7, 9, 3, 45) cells respectively. Fig. 3c is a graph showing that leptin depolarizes POMC cells by activating a 10 nonspecific cation current. The figure is representative of the response in 10 cells. Fig. 3d is a graph showing that leptin decreases the frequency of IPSCs in POMC cells. The figure is an example of 5 cells in which leptin (100 nM) decreased the frequency of IPSCs. Fig. 3e is a tracing demonstrating that leptin had no effect on 5 adjacent non-fluorescent ARC neurons. Fig. 3f is a tracing showing that leptin 15 hyperpolarized 5 non-fluorescent ARC neurons. Fig. 4 is a set of images showing that the GABAergic inputs to POMC cells are from NPY neurons that co-express GABA. Fig. 4a is a graph showing that NPY decreases the frequency of mini IPSCs in POMC neurons. Fig. 4b is a graph 20 demonstrating that D-Trp*-IMSH (7nM), a dose that selectively activates MC3-R, increases the frequency of GABAergic IPSCs in POMC neurons. Fig. 4c is a tracing showing that D-Trpg-'yMSH hyperpolarizes POMC neurons. Figs. 4a, 4b and 4c are representative. Fig. 4d is a set of digital images demonstrating that expression of NPY in nerve terminals adjacent to POMC neurons in the ARC. NPY nerve 25 terminals (black, arrowheads); POMC neuronal soma (grey). Scale bar, 10 pm. Fig. 4e is a digital image showing expression of GABA and NPY in nerve terminals synapsing onto POMC neurons in the ARC. GABA immunoreactivity (10 un gold particles, arrowheads without tail) and NPY immunoreactivity (25 nm gold particles, arrows with tail) are in separate vesicle populations co-localized within synaptic 30 boutons that make direct contact with the soma of POMC neurons (DAB contrasted with uranyl acetate and lead citrate, diffuse black in cytoplasm). Scale bar, 1 pim. Fig. 4f is a diagram of the model of NPY/GABA and POMC neurons in the ARC.

-6 Fig. 5 is a set of graphs relating to the feeding response to PYY 3

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36 in rats. Fig. 5a is a bar graph of dark-phase feeding tabulating food intake after intraperitoneal injection of PYY 3

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36 . Freely feeding rats were injected with PYY 3 -3 6 5 at the doses indicated (jg/i 00g), or saline, just prior to 'lights off' and 4-hour cumulative food intake was measured. Results are the mean ± s.e.m. (n = 8 per group), * = p <0.05, ** = p < 0.01, *** = <0.001 compared to saline. Fig. 5b is a bar graph of food intake after intraperitoneal injection of PYY 3

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3 6 . Fasted rats were injected with PYY 3

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3 6 at the doses indicated (pg/i 00g), or saline, and 4-hour 10 cumulative food intake was measured. Results are shown as the mean ± s.e.m. (n 8 per group), *= p < 0.05, **= p < 0.01, **=< 0.001 compared to saline. Fig. 5c is a bar graph of cumulative food intake after intraperitoneal injection of saline or

PYY

3

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3 a. Fasted rats were injected with either saline (closed bars) or PYY 3

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36 Spig/100g (open bars) and cumulative food intake measured at the time points 15 indicated. Results are expressed as mean I s.e.m. (n = 12 per group), **= p < 0.01 compared to saline. Fig. 5d is a line graph of body weight gain during chronic treatment with PYY 3

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3 6 . Rats were injected intraperitoneally with PYY 3 .3 5pg/100g (open squares) or saline (filled inverted triangles) twice daily for 7 days. Body weight gain was calculated each day. Results are expressed as mean ± s.e.m. (n = 12 20 per group) ** = p <0.01 compared to saline. Fig. 6 is a set of digital images of c-fos expression in Pomc-EGFP mice. Figs. 6a and 6b are digital images of representative sections (bregma -1.4 mm 22 ) of c-fos expression in the arcuate nucleus of Pomc-EGFP mice response to 25 intraperitoneal saline (Fig. 6a) or PYY 3

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3 6 (5ig/100g) (Fig. 6b). Scale bar 100 pm. 3V, third ventricle; Arc, arcuate nucleus. Figs. 6c and 6d are digital images of representative sections showing POMC-EGFP neurons (Fig. 6c) and c-fos immunoreactivity (Fig. 6d) either co-localizing (bright arrows) or alone (single darker arrow). Scale bar 25 pm. 30 Fig. 7 is a set of bar graphs relating to intra-arcuate PYY 3

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3 6 in rats and feeding effects of IP PYY 3

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3 6 in Y2r- null mice. Fig. 7a is a bar graph of food intake -7 following intra-arcuate PYY 3

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36 injection. Fasted rats were injected with saline or

PYY

3

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36 into the arcuate nucleus at the doses indicated. Post-injection 2-hour food intake was measured, ** = p < 0.01 compared to saline. Figs. 7b and 7c are bar graphs of feeding response to PYY 3

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36 in Y2r-null mice following IP administration: 5 wild type littermates mice (Fig. 7b) and Y2r-null mice (Fig. 7c), fasted for 24 hours, were injected with PYY 3

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36 at the doses indicated (pig/I 00g), or saline, and 4-hour cumulative food intake was measured. Results are the mean ± s.e.m. (n= 5 per group), * = p < 0.05, ** = p < 0.01 compared to saline. Fig. 8 is a set of images relating to the electrophysiological and neuropeptide 10 responses to PYY 3

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36 and Y2A. Fig. 8a is a tracing showing the effect of PYY 3

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36 (10 nM) on the frequency of action potentials in POMC neurons (whole-cell configuration recordings; n= 22) * p< 0.05. PYY 3 -3 8 was administered at time D for 3 minutes; baseline, -3 to 0 minute; PYY 3 .36, 2-5 minutes; and wash-out, 8-11 minutes. Inset shows a representative recording of membrane potential and action 15 potential frequency. Fig. 8b is a graph of the effect if PYY 3

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38 (10nM) on the frequency of action potentials in loose cell-attached patch recordings (n=8). Data from individual cells were normalized to the firing rate for the 200s before PYY 3 -- 3 8 addition. Fig. 8c is a tracing and a graph of the effect of PYY 3

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36 (50nM) on spontaneous IPSCs onto POMC neurons (n=13). Inset shows a representative 20 recording of IPSCs before and after PYY 3 .36 (50nM), respectively. Results in Fig. 8a-8c are expressed as mean V s.e.m. Fig. 8d and 8e are bar graphs showing NPY (Fig. 8d) and %-MSH (Fig. 8e) released from hypothalamic explants in response to Y2A. Hypothalamic slices were incubated with artificial CSF (aCSF), with or without 50nM Y2A, for 45 minutes. Results are expressed as mean V s.e.m. (n=40); 25 = p<0.01; *** = p<0.001 compared to saline. Fig. 9 is a set of graphs showing the effect of PYY3- 36 infusion on appetite and food intake in human subjects. Fig. 9a is a graph of the calorie intake from a "free-choice" buffet meal 2 hours after infusion with saline or PYY3- 36 . The thin lines indicate individual changes in calorie intake for each subject between saline 30 and PYY 3 -36 administration. The thick line represents mean change between the two infusions (n= 12). Fig. 9b is a graph of the 24-hour calorie intake following -8 infusion with saline or PYY 3

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36 . Total calorie intake, as assessed by food diaries, is shown for the 24-hour period following either saline or PYY 3

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36 infusion. Data is given as mean ± s.e.m. (n = 12), ***= p < 0.0001 compared to saline. Fig. 9c is a graph of the appetite score (relative scale). Visual analogue scores (Raben et al., Br. 5 J. Nutr. 73, 517-30, 1995) show perceived hunger during and after infusions. The results are presented as change from baseline scores and are the mean L s.e.m. for all 12 subjects. Fig. 10 shows plasma PYY 3

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36 levels following subcutaneous administration of 10 nmols PYY 3 -36 (broken line) and 20 nmols PYY 3

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3 6 (solid line). 10 Fig. 11 shows the effect on I-hour food intake in non-fasted rats of IP administration of GLP- 1 in the presence or absence of concomitant exendin 9-39 in the arcuate nucleus of the rat. S = Saline, G = GLP- 1 (25 nmol/kg), and Ex = exendin 9-39 (20 nmoles/kg). Fig. 12 shows the effects of co-administration of PYY 3

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36 and GLP-1 on 1 15 hour food intake in non-fasted rats injected (intraperitoneally) prior to the onset of the dark phase. IP GLP-1 (30pg/kg = lxG or 60pg/kg = 2xG in 500 pl saline) or IP

PYY

3 a 36 (3pg/kg = 1xP or 6pg/kg = 2xP in 500 pl saline). Co-administration (P+G group) is GLP-1 30pg/kg and PYY 3

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36 3pg/kg combined. 20 SEQUENCE LISTING The nucleic and amino acid sequences listed in the accompanying sequence listing are shown using standard letter abbreviations for nucleotide bases, and three letter code for amino acids, as defined in 37 C.F.R. 1.822. Only one strand of each 25 nucleic acid sequence is shown, but the complementary strand is understood as included by any reference to the displayed strand. DETAILED DESCRIPTION 30 . Abbreviations a-MSH: alpha melanocortin stimulating hormone -9 Are: arcuate nucleus EPSP: excitatory postsynaptic potential GABA: yaminobutyric acid GFP, EGFP: green fluorescent protein 5 GLP-1: glucagons-like peptide-1 ICV: intracerebroventricular IP: intraperitoneal IPSC: inhibitory postsynaptic current kb: kilobase 10 kg: kilogram MOP-R: y-opiod receptor MV: millivolts NPY: neuropeptide Y Oxm: oxyntomodulin 15 pmol: picomole POMC: proopiomelanocortin RIA: radioimmunoassay RPA: RNase protection assay s.e.m: standard error of the mean 20 TH: tyrosine hydroxylase gM: micromolar V: volts Y2A: N-acetyl (Leu 2, Leu ") NPY (24-36) 25 II. Terms Unless otherwise noted, technical terms are used according to conventional usage. Definitions of common terms in molecular biology may be found in Benjamin Lewin, Genes V, published by Oxford University Press, 1994 (ISBN 0-19 854287-9); Kendrew et al. (eds.), The Encyclopedia ofMolecular Biology, published 30 by Blackwell Science Ltd., 1994 (ISBN 0-632-02182-9); and Robert A. Meyers (ed.), Molecular Biology and Biotechnology: a Comprehensive Desk Reference, published by VCH Publishers, Inc., 1995 (ISBN 1-56081-569-8).

-10 In order to facilitate review of the various embodiments of this disclosure, the following explanations of specific terms are provided: Action potential: A rapidly propagated electrical message that speeds along 5 an axon of a neuron and over the surface membrane of many muscle and glandular cells. In axons they are brief, travel at constant velocity, and maintain a constant amplitude. Like all electrical messages of the central nervous system, the action potential is a membrane potential change caused by the flow of ions through ion channels in the membrane. In one embodiment, an action potential is a regenerative 10 wave of sodium permeability. Animal: Living multi-cellular vertebrate organisms, a category that includes, for example, mammals and birds. The term mammal includes both human and non-human mammals. Similarly, the term "subject" includes both human and veterinary subjects. 15 Anorexia: A lack or loss of the appetite for food. In one embodiment, anorexia is a result of "anorexia nervosa." This is an eating disorder primarily affecting females, usually with onset in adolescence, characterized by refusal to maintain a normal minimal body weight, intense fear of gaining weight or becoming obese, and a disturbance of body image resulting in a feeling of being fat or having 20 fat in certain areas even when extremely emaciated, undue reliance on body weight or shape for self-evaluation, and amenorrhea. Associated features often include denial of the illness and resistance to psychotherapy, depressive symptoms, markedly decreased libido, and obsessions or peculiar behavior regarding food, such as hoarding. The disorder is divided into two subtypes, a restricting type, in which 25 weight loss is achieved primarily through diet or exercise, and a binge eating/purging type, in which binge eating or purging behavior also occur regularly. Antagonist: A substance that tends to nullify the action of another, as an agent that binds to a cell receptor without eliciting a biological response, blocking binding of substances that could elicit such responses. 30 Appetite: A natural desire, or longing for food. In one embodiment, appetite is measured by a survey to assess the desire for food. Increased appetite generally leads to increased feeding behavior.

-11 Appetite Suppressants: Compounds that decrease the desire for food. Commercially available appetite suppressants include, but are not limited to, amfepramone (diethylpropion), phentermine, mazindol and phenylpropanolamine fenfluramine, dexfenfluramine, and fluoxetine. 5 Binding: A specific interaction between two molecules, such that the two molecules interact. Binding can be specific and selective, so that one molecule is bound preferentially when compared to another molecule. In one embodiment, specific binding is identified by a disassociation constant (Kd). Body Mass Index (BMI): A mathematical formula for measuring body 10 mass, also sometimes called Quetelet's Index. BMI is calculated by dividing weight (in kg) by height (in meters 2 ). The current standards for both men and women accepted as "normal" are a BMI of 20-24.9 kg/m 2 . In one embodiment, a BMI of greater than 25 kg/m2 can be used to identify an obese subject. Grade I obesity corresponds to a BMI of 25-29.9 kg/m 2 . Grade I obesity corresponds to a BMI of 15 30-40 kg/n 2 ; and Grade 11I obesity corresponds to a BMI greater than 40 kg/m2 (Jequier,Am. J Clin. Nutr. 45:1035-47, 1987). Ideal body weight will vary among species and individuals based on height, body build, bone structure, and sex. c-fos: The cellular homologue of the viral v-fos oncogene found in FBJ (Finkel-Biskis-Jinkins) and FBR murine osteosarcoma viruses (MSV). The human 20 fos gene maps to chromosome 14q21-q31. Human fos has been identified as TIS 28. C-fos is thought to have an important role in signal transduction, cell proliferation, and differentiation. It is a nuclear protein which, in combination with other transcription factors (for example, jun) acts as a trans-activating regulator of 25 gene expression. C-fos is an immediate early response gene, which are believed to play a key role in the early response of cells to growth factors. C-fos is involved also in the control of cell growth and differentiation of embryonic hematopoietic cells and neuronal cells. The human c-fos coding amino acid and nucleic sequences are known (e.g., see Verma et al., Cold Spring Harb. Symp. Quant. Biol. 51, 949, 30 1986; GenBank Accession Nos. K00650 and M16287, and is available on the internet).

-12 Caloric intake or calorie intake: The number of calories (energy) consumed by an individual. Calorie: A unit of measurement in food. A standard calorie is defined as 4.184 absolute joules, or the amount of energy it takes to raise the temperature of 5 one gram of water from 15 to 16* C (or1/100th the amount of energy needed to raise the temperature of one gram of water at one atmosphere pressure from 0* C to 1000 C), food calories are actually equal to 1,000 standard calories (1 food calorie = 1 kilocalorie). Conservative variation: The replacement of an amino acid residue by 10 another, biologically similar residue. Examples of conservative variations include the substitution of one hydrophobic residue such as isoleucine, valine, leucine or methionine for another, or the substitution of one polar residue for another, such as the substitution of arginine for lysine, glutamic for aspartic acid, or glutamine for asparagine, and the like. The term "conservative variation" also includes the use of a 15 substituted amino acid in place of an unsubstituted parent amino acid provided that antibodies raised to the substituted polypeptide also immunoreact with the unsubstituted polypeptide. Non-limiting examples of conservative amino acid substitutions include those listed below: 20 Original Residue Conservative Substitutions Ala Ser Arg Lys 25 Asn Gln, His Asp Glu Cys Ser Gin Asn Glu Asp 30 His Asn; Gin Ile Leu, Val Leu Ile; Val Lys Arg; Gln; Glu Met Leu; fle 35 Phe Met; Leu; Tyr Ser Thr Thr Ser Trp Tyr -13 Tyr Trp; Phe Val Ile; Leu Depolarization: An increase in the membrane potential of a cell. Certain 5 stimuli reduce the charge across the plasma membrane. These can be electrical stimuli (which open voltage-gated channels), mechanical stimuli (which activate mechanically-gated channels) or certain neurotransmitters (which open ligand-gated channels). In each case, the facilitated diffusion of sodium into the cell increases the resting potential at that spot on the cell creating an excitatory postsynaptic potential 10 (EPSP). Depolarizations can also be generated by decreasing the frequency of inhibitory postsynaptic currents (IPSCs), these are due to inhibitory neurotransmitters facilitating the influx of chloride ions into the cell, creating an IPSC. If the potential is increased to the threshold voltage (about -50 mV in mammalian neurons), an action potential is generated in the cell. 15 Diabetes: A failure of cells to transport endogenous glucose across their membranes either because of an endogenous deficiency of insulin and/or a defect in insulin sensitivity. Diabetes is a chronic syndrome of impaired carbohydrate, protein, and fat metabolism owing to insufficient secretion of insulin or to target tissue insulin resistance. It occurs in two major forms: insulin-dependent diabetes 20 mellitus (IDDM, type I) and non-insulin dependent diabetes mellitus (NIDDM, type II) which differ in etiology, pathology, genetics, age of onset, and treatment. The two major forms of diabetes are both characterized by an inability to deliver insulin in an amount and with the precise timing that is needed for control of glucose homeostasis. Diabetes type I, or insulin dependent diabetes mellitus 25 (IDDM) is caused by the destruction of cells, which results in insufficient levels of endogenous insulin. Diabetes type II, or non-insulin dependent diabetes, results from a defect in both the body's sensitivity to insulin, and a relative deficiency in insulin production. Exendin: A 39-amino acid peptide isolated from the salivary glands of the 30 Gila monster (Heloderma suspectum) (Eng J et al J Biol Chem 267:7402-7405, 1992), see SEQ ID NO:341. Exendin is an example of an agonist at the GLP-1 receptor. Molecules derived from exendin-4 and that also have GLP-l agonist activity are further examples of GLP-1 agonists.

-14 Food Intake: The amount of food consumed by an individual. Food intake can be measured by volume or by weight. In one embodiment, food intake is the total amount of food consumed by an individual. In another embodiment, food intake is the amount of proteins, fat, carbohydrates, cholesterol, vitamins, minerals, 5 or any other food component, of the individual. "Protein intake" refers to the amount of protein consumed by an individual. Similarly, "fat intake," "carbohydrate intake," "cholesterol intake," "vitamin intake," and "mineral intake" refer to the amount of proteins, fat, carbohydrates, cholesterol, vitamins, or minerals consumed by an individual. 10 Glucagon-like peptide-1: Peptides produced by processing of preproglucogon, which is a 160 amino acid polypeptide, in the central nervous system (CNS) and the intestine. GLP-1 (1-37) is the initial product. GLP-1 (1-37) is amidated by post-translational processing to yield GLP-1 (1-36) NH, or is enzymatically processed to give GLP-1 (7-37) (SEQ ID NO: 338). GLP-1 (7-37) 15 can be amidated to give GLP-l (7-36) amide (SEQ ID NO: 339), which is the most biologically active form. GLP-1 is released into the circulation in response to nutrient intake. Intestinal cells secrete GLP-1 (7-37) and GLP-1 (7-36) amide in a ratio of 1 to 5. GLP-1 receptors are found in the brainstem, arcuate nucleus and 20 paraventricular nucleus. Physiological actions of GLP- 1 in man include stimulation of insulin release, suppression of gastric acid secretion and slowing of gastric emptying. A GLP-l agonist is a peptide, small molecule, or chemical compound that preferentially binds to the GLP-1 receptor and stimulate sthe same biological 25 activity as GLP-1. In one embodiment, an agonist for the GLP- I receptor binds to the receptor with an equal or greater affinity than a GLP-1 peptide. In another embodiment, an agonist selectively binds the GLP-1 receptor, as compared to binding to another receptor. One of skill in the art can readily determine the dissociation constant (K,4) 30 value of a given compound. This value is dependent on the selectivity of the compound tested. For example, a compound with a Kd which is less than 10 nM is generally considered an excellent drug candidate. However, a compound that has a -15 lower affinity, but is selective for the particular receptor, can also be a good drug candidate. In one specific, non-limiting example, an assay, such as a competition assay, is used to determine if a compound of interest is a GLP-1 receptor agonist. GLP-1 agonists include GLP-1 related peptides and peptides that result from 5 natural or synthetic enzymatic or chemical processing of a GLP-1 peptide or a related peptide. Hyperpolarization: A decrease in the membrane potential of a cell. Inhibitory neurotransmitters inhibit the transmission of nerve impulses via hyperpolarization. This hyperpolarization is called an inhibitory postsynaptic 10 potential (IPSP). Although the threshold voltage of the cell is unchanged, a hyperpolarized cell requires a stronger excitatory stimulus to reach threshold. Inhibitory Postsynaptic Current: A current that inhibits an electrophysiological parameter of a postsynaptic cell. The potential of a postsynaptic cell can be analyzed to determine an effect on a presynaptic cell. In 15 one embodiment, the postsynaptic cell is held in voltage clamp mode, and postsynaptic currents are recorded. If necessary, antagonists of other classes of current can be added. In one specific, non-limiting example, to record GABAergic IPSCs, blockers of excitatory channels or receptors can be added. The instantaneous frequency over time is then determined. 20 In one embodiment, IPSCs give a measure of the frequency of GABA release from an NPY neuron. Thus, as NPY neurons release GABA onto POMC neurons, measurement of IPSC frequency is a gauge of the inhibitory tone that POMC neurons are receiving, and can be used to assess the effect of an agonist of PYY. Membrane potential: The electrical potential of the interior of the cell with 25 respect to the environment, such as an external bath solution. One of skill in the art can readily assess the membrane potential of a cell, such as by using conventional whole cell techniques. Activation of a cell is associated with less negative membrane potentials (for example shifts from about -50 mV to about -40 mV). These changes in potential increase the likelihood of action potentials, and thus lead 30 to an increase in the rate of action potentials. The rate of action potentials can be assessed using many approaches, such as using conventional whole cell access, or using, for example, perforated-patch whole- -16 cell and cell-attached configurations. In each event the absolute voltage or current is not assessed, rather the frequency of rapid deflections characteristic of action potentials is assessed, as a function of time (therefore this frequency is an instantaneous frequency, reported in "bins"). This time component can be related to 5 the time at which a compound, such as a PYY agonist, is applied to the bath to analyze the effect of the compound, such as the PYY agonist, on action potential firing rate. Neuropeptide Y (NPY): A 36-amino acid peptide that is a neuropeptide identified in the mammalian brain. NPY is believed to be an important regulator in 10 both the central and peripheral nervous systems and influences a diverse range of physiological parameters, including effects on psychomotor activity, food intake, central endocrine secretion, and vasoactivity in the cardiovascular system. High concentrations of NPY are found in the sympathetic nerves supplying the coronary, cerebral, and renal vasculature and have contributed to vasoconstriction. NPY 15 binding sites have been identified in a variety of tissues, including spleen, intestinal membranes, brain, aortic smooth muscle, kidney, testis, and placenta. In addition, binding sites have been reported in a number of rat and human cell lines. Neuropeptide Y (NPY) receptor has structure/activity relationships within the pancreatic polypeptide family. This family includes NPY, which is synthesized 20 primarily in neurons; peptide YY (PYY), which is synthesized primarily by endocrine cells in the gut; and pancreatic polypeptide (PP), which is synthesized primarily by endocrine cells in the pancreas. These 36 amino acid peptides have a compact helical structure involving an amino acid structure, termed a "PP-fold" in the middle of the peptide. 25 NPY binds to several receptors, including the Y1, Y2, Y3, Y4 (PP), Y5, Y6, and Y7 receptors. These receptors are recognized based on binding affinities, pharmacology, and sequence (if known). Most, if not all of these receptors are G protein-coupled receptors. The Y1 receptor is generally considered to be postsynaptic and mediates many of the known actions of neuropeptide Y in the 30 periphery. Originally, this receptor was described as having poor affinity for C terminal fragments of neuropeptide Y, such as the 13-36 fragment, but interacts with -17 the full length neuropeptide Y and peptide YY with equal affinity (e.g., see PCT publication WO 93/09227). Pharmacologically, the Y2 receptor is distinguished from Y1 by exhibiting affinity for C-terminal fragments of neuropeptide Y. The Y2 receptor is most often 5 differentiated by the affinity of neuropeptide Y(13-36), although the 3-36 fragment of neuropeptide Y and peptide YY provides improved affinity and selectivity (see Dumont et al., Societyfor Neuroscience Abstracts 19:726, 1993). Signal transmission through both the Yl and the Y2 receptors are coupled to the inhibition of adenylate cyclase. Binding to the Y-2 receptor was also found to reduce the 10 intracellular levels of calcium in the synapse by selective inhibition of N-type calcium channels. In addition, the Y-2 receptor, like the Yl receptors, exhibits differential coupling to second messengers (see U.S. Patent No. 6,355,478). Y2 receptors are found in a variety of brain regions, including the hippocampus, substantia nigra-lateralis, thalamus, hypothalamus, and brainstem. The human, 15 murine, monkey and rat Y2 receptors have been cloned (e.g., see U.S. Patent No. 6,420,352 and U.S. Patent No. 6,355,478). A Y2 receptor agonist is a peptide, small molecule, or chemical compound that preferentially binds to the Y2 receptor and stimulates intracellular signaling. In one embodiment, an agonist for the Y2 receptor binds to the receptor with an equal 20 or greater affinity than NPY. In another embodiment, an agonist selectively binds the Y2 receptor, as compared to binding to another receptor. One of skill in the art can readily determine the dissociation constant (Kd) value of a given compound. This value is dependent on the selectivity of the compound tested. For example, a compound with a Kd which is less than 10 nM is 25 generally considered an excellent drug candidate. However, a compound that has a lower affinity, but is selective for the particular receptor, can also be a good drug candidate. In one specific, non-limiting example, an assay, such as a competition assay, is used to determine if a compound of interest is a Y2 receptor agonist. Assays useful for evaluating neuropeptide Y receptor antagonists are also well 30 known in the art (see U.S. Patent No. 5,284,839, which is herein incorporated by reference, and Walker et al., Journal ofNeurosciences 8:2438-2446, 1988).

-18 Normal Daily Diet: The average food intake for an individual of a given species. A normal daily diet can be expressed in terms of caloric intake, protein intake, carbohydrate intake, and/or fat intake. A normal daily diet in humans generally comprises the following: about 2,000, about 2,400, or about 2,800 to 5 significantly more calories. In addition, a normal daily diet in humans generally includes about 12 g to about 45 g of protein, about 120 g to about 610 g of carbohydrate, and about 11 g to about 90 g of fat. A low calorie diet would be no more than about 85%, and preferably no more than about 70%, of the normal caloric intake of a human individual. 10 In animals, the caloric and nutrient requirements vary depending on the species and size of the animal. For example, in cats, the total caloric intake per pound, as well as the percent distribution of protein, carbohydrate and fat varies with the age of the cat and the reproductive state. A general guideline for cats, however, is 40 cal/lb/day (18.2 cal/kg/day). About 30% to about 40% should be protein, 15 about 7% to about 10% should be from carbohydrate, and about 50% to about 62.5% should be derived from fat intake. One of skill in the art can readily identify the normal daily diet of an individual of any species. Obesity: A condition in which excess body fat may put a person at health risk (see Barlow and Dietz, Pediatrics 102:E29, 1998; National Institutes of Health, 20 National Heart, Lung, and Blood Institute (NHLBI), Obes. Res. 6 (suppl. 2):5 1S 209S, 1998). Excess body fat is a result of an imbalance of energy intake and energy expenditure. In one embodiment, the Body Mass Index (BMI) is used to assess obesity. In one embodiment, a BMI of 25.0 kg/m 2 to 29.9 kg/m 2 is overweight, while a BMI of 30 kg/m 2 is obese. 25 In another embodiment, waist circumference is used to assess obesity. In this embodiment, in men a waist circumference of 102 cm or more is considered obese, while in women a waist circumference of 89 cm or more is considered obese. Strong evidence shows that obesity affects both the morbidity and mortality of individuals. For example, an obese individual is at increased risk for heart disease, 30 non-insulin dependent (type 2) diabetes, hypertension, stroke, cancer (e.g. endometrial, breast, prostate, and colon cancer), dyslipidemia, gall bladder disease, -19 sleep apnea, reduced fertility, and osteoarthritis, amongst others (see Lyznicki et al., Am. Fam. Phys. 63:2185, 2001). Overweight: An individual who weighs more than their ideal body weight. An overweight individual can be obese, but is not necessarily obese. In one 5 embodiment, an overweight individual is any individual who desires to decrease their weight. In another embodiment, an overweight individual is an individual with a BMI of 25.0 kg/m 2 to 29.9 kg/m 2 Oxyntomodulin: A further peptide produced by post-translational processing of preproglucagon in the CNS and intestine, see SEQ ID NO: 340.. 10 Agonists of oxyntomodulin may be identified as described above for agonists of GLP-1 and of NPY. include oxyntomodulins of other species and also modified sequences, for example, sequences in which The term OXM used in this text also covers any analogue of the above OXM sequence, wherein the histidine residue at position 1 is maintained or replaced by an aromatic moiety carrying a positive 15 charge or a derivative thereof, preferably wherein the moiety is an amino acid, more preferably wherein it is a histidine derivative, while 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 or 22 of the other amino acids in the above OXM sequence can be independently replaced by any other independently chosen amino acid, with the exception of histidine in position 1. 20 Any one or more (to 22) other alpha-amino acid residue in the sequence can be independently replaced by any other one alpha-amino acid residue. Preferably, any amino acid residue other than histidine is replaced with a conservative replacement as well known in the art i.e. replacing an amino acid with one of a similar chemical type such as replacing one hydrophobic amino acid with another. 25 As discussed above, 1 to 22 of the amino acids can be replaced. In addition to the replacement option above, this may be by a non-essential or modified or isomeric form of an amino acid. For example, 1 to 22 amino acids can be replaced by an isomeric form (for example a D-amino acid), or a modified amino acid, for example a nor-amino acid (such as norleucine or norvaline) or a non-essential amino 30 acid (such as taurine). Furthermore, 1 to 22 amino acids may be replaced by a con'esponding or different amino acid linked via its side chain (for example gamma- -20 linked glutamic acid). For each of the replacements discussed above, the histidine residue at position 1 is unaltered or defined above. In addition, 1, 2, 3, 4 or 5 of the amino acid residues can be removed from the OXM sequence with the exception of histidine at the 1 position (or as defined 5 above). The deleted residues may be any 2, 3, 4 or 5 contiguous residues or entirely separate residues. The C-terminus of the OXM sequence may be modified to add further amino acid residues or other moieties. Pancreatic Polypeptide: A 36 amino acid peptide produced by the pancreas 10 that is has homology to PYY and NPY. Peripheral Administration: Administration outside of the central nervous system. Peripheral administration does not include direct administration to the brain. Peripheral administration includes, but is not limited to intravascular, intramuscular, subcutaneous, inhalation, oral, rectal, transdermal or intra-nasal 15 administration Polypeptide: A polymer in which the monomers are amino acid residues which are joined together through amide bonds. When the amino acids are alpha amino acids, either the L-optical isomer or the D-optical isomer can be used, the L isomers being preferred. The terms "polypeptide" or "protein" as used herein are 20 intended to encompass any amino acid sequence and include modified sequences such as glycoproteins. The term "polypeptide" is specifically intended to cover naturally occurring proteins, as well as those which are recombinantly or synthetically produced. The term "polypeptide fragment" refers to a portion of a polypeptide, for example such a fragment which exhibits at least one useful 25 sequence in binding a receptor. The term "functional fragments of a polypeptide" refers to all fragments of a polypeptide that retain an activity of the polypeptide. Biologically functional peptides can also include fusion proteins, in which the peptide of interest has been fused to another peptide that does not decrease its desired activity. 30 PYY: A peptide YY polypeptide obtained or derived from any species. Thus, PYY includes the human full length polypeptide (as set forth in SEQ ID NO: 1) and species variations of PYY, including e.g. marine, hamster, chicken, bovine, -21 rat, and dog PYY (SEQ ID NOS: 5-12). In one embodiment, PYY agonists do not include NPY. PYY also includes PYY 3 .3. A "PYY agonist" is any compound which binds to a receptor that specifically binds PYY, and elicits an effect of PYY. In one embodiment, a PYY agonist is a compound that affects food intake, caloric 5 intake, or appetite, and/or which binds specifically in a Y receptor assay or competes for binding with PYY, such as in a competitive binding assay with labeled PYY. PYY agonists include, but are not limited to, compounds that bind to the Y2 receptor. Substantially purified: A polypeptide which is substantially free of other 10 proteins, lipids, carbohydrates or other materials with which it is naturally associated. For example, the polypeptide may be at least 50%, 80% or 90% free of other proteins, lipids, carbohydrates or other materials with which it is naturally associated. Therapeutically effective amount: A dose sufficient to prevent 15 advancement, or to cause regression of a disorder, or which is capable of relieving a sign or symptom of a disorder, or which is capable of achieving a desired result. In several embodiments, a therapeutically effect of PYY or an agonist thereof is an amount sufficient to inhibit or halt weight gain, or an amount sufficient to decrease appetite, or an amount sufficient to reduce caloric intake or food intake or increase 20 energy expenditure. Unless otherwise explained, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. The singular terms "a," "an," and "the" include 25 plural referents unless context clearly indicates otherwise. Similarly, the word "or" is intended to include "and" unless the context clearly indicates otherwise. It is further to be understood that all base sizes or amino acid sizes, and all molecular weight or molecular mass values, given for nucleic acids or polypeptides are approximate, and are provided for description. Although methods and materials 30 similar or equivalent to those described herein can be used in the practice or testing of this disclosure, suitable methods and materials are described below. The term "comprises" means "includes." All publications, patent applications, patents, and -22 other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including explanations of terms, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting. 5 Methods for Altering Food Intake, Appetite, Caloric Intake and Energy Expenditure A method is disclosed herein for reducing food intake by peripherally administering to a subject a therapeutically effective amount of PYY or an agonist of 10 PYY and GLP-1 or an agonist thereof. In one embodiment, administration of PYY, or an agonist of PYY, and GLP-1 or an agonist thereof, results in a decrease in the amount, either the total weight or the total volume of food. In other embodiment, administration of PYY, or an agonist thereof, and GLP-1 or an agonist thereof, results in a decrease of the intake of a food component, such as a decrease in the 15 ingestion of lipids, carbohydrates, cholesterol, or proteins. In the any of the methods disclosed herein, a preferred compound, PYY 3.36 can be administered. This disclosure includes the corresponding uses of PYY or an agonist thereof and GLP-1 or an agonist thereof, for the manufacture of a medicament or medicaments for the purposes set herein, and includes the use of PYY 3

.

36 . 20 A method is also disclosed herein for reducing caloric intake by peripherally administering to a subject a therapeutically effective amount of PYY or an agonist of PYY and GLP- I or an agonist thereof. In one embodiment, total caloric intake is reduced by peripheral administration of a therapeutically effective amount of PYY and GLP-1 or an agonist thereof. In other embodiments, the caloric intake from the 25 ingestion of a specific food component, such as, but not limited to, the ingestion of lipids, carbohydrates, cholesterol, or proteins, is reduced. In an additional embodiment, a method is disclosed herein for reducing appetite by administering a therapeutically effective amount of PYY or an agonist thereof and GLP-l or an agonist thereof. Appetite can be measured by any means 30 known to one of skill in the art. For example, decreased appetite can be assessed by a psychological assessment. In this embodiment, administration of PYY and the other agent(s) results in a change in perceived hunger, satiety, and/or fullness.

-23 Hunger can be assessed by any means known to one of skill in the art. In one embodiment, hunger is assessed using psychological assays, such as by an assessment of hunger feelings and sensory perception using a questionnaire, such as, but not limited to, a Visual Analog Score (VAS) questionnaire (see the Examples 5 section). In one specific, non-limiting example, hunger is assessed by answering questions relating to desire for food, drink, prospective food consumption, nausea, and perceptions relating to smell or taste. In a further embodiment, a method is disclosed herein for altering energy metabolism in a subject. The method includes peripherally administering a 10 therapeutically effective amount of PYY or an agonist thereof and GLP-l or an agonist thereof, to the subject, thereby altering energy expenditure. Energy is burned in all physiological processes. The body can alter the rate of energy expenditure directly, by modulating the efficiency of those processes, or changing the number and nature of processes that are occurring. For example, during 15 digestion the body expends energy moving food through the bowel, and digesting food, and within cells, the efficiency of cellular metabolism can be altered to produce more or less heat. In a further embodiment a method is disclosed herein for any and all manipulations of the arcuate circuitry described in this application, that alter food intake coordinately and reciprocally alter energy expenditure. Energy 20 expenditure is a result of cellular metabolism, protein synthesis, metabolic rate, and calorie utilization. Thus, in this embodiment, peripheral administration of PYY and administration of GLP-1 or an agonist thereof, results in increased energy expenditure, and decreased efficiency of calorie utilization. In one embodiment, a therapeutically effective amount of PYY or an agonist and GLP-1 or an agonist 25 thereof, thereof is administered to a subject, thereby increasing energy expenditure. In several embodiments, PYY (e.g., PYY 3

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36 ) or an agonist thereof and GLP 1 or an agonist thereof, are used for weight control and treatment, reduction or prevention of obesity, in particular any one or more of the following: preventing and reducing weight gain; inducing and promoting weight loss; and reducing obesity as 30 measured by the Body Mass Index. The disclosure further relates to the use of PYY or an agonist and GLP-1 or an agonist thereof, in control of any one or more of appetite, satiety and hunger, in particular any one or more of the following: -24 reducing, suppressing and inhibiting appetite; inducing, increasing, enhancing and promoting satiety and sensations of satiety; and reducing, inhibiting and suppressing hunger and sensations of hunger. The disclosure further relates to the use of PYY an agonist thereof and GLP-l or an agonist thereof, in maintaining any one or more of 5 a desired body weight, a desired Body Mass Index, a desired appearance and good health. The subject can be any subject, including both human and veterinary mammalian subjects. Thus, the subject can be a human, or can be a non-human primate, a farm animal such as swine, cattle, and poultry, a sport animal or pet such 10 as dogs, cats, horses, hamsters, rodents, or a zoo animal such as lions, tigers, or bears. Obesity is currently a poorly treatable, chronic, essentially intractable metabolic disorder. A therapeutic drug useful in weight reduction of obese persons could have a profound beneficial effect on their health. Thus, the subject can be, but 15 is not limited to, a subject who is overweight or obese. In one embodiment, the subject has, or is at risk of having, a disorder wherein obesity or being overweight is a risk factor for the disorder. Disorders of interest include, but are not limited to, cardiovascular disease, (including, but not limited to, hypertension, atherosclerosis, congestive heart failure, and dyslipidemia), stroke, gallbladder disease, 20 osteoarthritis, sleep apnea, reproductive disorders such as, but not limited to, polycystic ovarian syndrome, cancers (e.g., breast, prostate, colon, endometrial, kidney, and esophagus cancer), varicose veins, acnthosis nigricans, eczema, exercise intolerance, insulin resistance, hypertension hypercholesterolemia, cholithiasis, osteoarthritis, orthopedic injury, insulin resistance (such as, but not limited to, type 2 25 diabetes and syndrome X) and tromboembolic disease (see Kopelman, Nature 404:635-43; Rissanen et al., British Med. J. 301, 835, 1990). Other associated disorders also include depression, anxiety, panic attacks, migraine headaches, PMS, chronic pain states, fibromyalgia, insomnia, impulsivity, obsessive compulsive disorder, and myoclonus. Obesity is a recognized risk factor 30 for increased incidence of complications of general anesthesia. (See e. g., Kopelnan, Nature 404:635-43, 2000). It reduces life span and carries a serious risk of co-morbidities listed above.

-25 Other diseases or disorders associated with obesity are birth defects (maternal obesity associated with increased incidence of neural tube defects), carpal tunnel syndrome (CTS), chronic venous insufficiency (CVI), daytime sleepiness, deep vein thrombosis (DVT), end stage renal disease (ESRD), gout, heat disorders, 5 impaired immune response, impaired respiratory function, infertility, liver disease, lower back pain, obstetric and gynecologic complications, pancreatititis, as well as abdominal hernias, acanthosis nigricans, endocrine abnormalities, chronic hypoxia and hypercapnia, dermatological effects, elephantitis, gastroesophageal reflux, heel spurs, lower extremity edema, manmegaly (causing considerable problems such as 10 bra strap pain, skin damage, cervical pain, chronic odors and infections in the skin folds under the breasts, etc.), large anterior abdominal wall masses (abdominal panniculitis with frequent panniculitis, impeding walking, causing frequent infections, odors, clothing difficulties, low back pain), musculoskeletal disease, pseudo tumor cerebri (or benign intracranial hypertension), and sliding hiatil hernia. 15 The present disclosure relates to treating, prcvcntion, aniciorating or alleviating conditions or disorders caused by, complicated by, or aggravated by a relatively high nutrient availability. By "condition or disorder which can be alleviated by reducing caloric (or nutrient) availability," it is meant any condition or disorder in a subject that is either caused by, complicated by, or aggravated by a 20 relatively high nutrient availability, or that can be alleviated by reducing nutrient availability, for example by decreasing food intake. Subjects who are insulin resistant, glucose intolerant, or have any form of diabetes mellitus (e.g., type 1, 2 or gestational diabetes) can also benefit from this disclosure. Such conditions or disorders are disorders associated with increased caloric 25 intake, insulin resistance, or glucose intolerance and include, but are not limited to, obesity, diabetes, including type 2 diabetes, eating disorders, insulin-resistance syndromes, and Alzheimer's disease. In another embodiment, the subject is a subject who desires weight loss, such as female and male subject who desire a change in their appearance. In yet a further 30 embodiment, the subject is a subject who desires decreased feelings of hunger, such as, but not limited to, a person involved in a lengthy task that requires a high level of -26 concentration (e.g., soldiers on active duty, air traffic controllers, or truck drivers on long distance routes, etc.). A suitable administration format may be best determined by the subject or by a medical practitioner. In one embodiment, the pharmaceutical compositions 5 that include PYY, or an agonist thereof, will preferably be formulated in unit dosage form, suitable for individual administration of precise dosages. An effective amount of PYY or an agonist thereof can be administered in a single dose, or in multiple doses, for example daily, during a course of treatment. In one embodiment, PYY is administered whenever the effect (e.g., appetite suppression, decreased food intake, 10 or decreased caloric intake) is desired. In another embodiment, PYY or an analog thereof is administered slightly prior to whenever the effect is desired, such as, but not limited to about 10 minutes, about 15 minutes, about 30 minutes, about 60 minutes, about 90 minutes, or about 120 minutes, prior to the time the effect is desired. In another embodiment, a time release formulation is utilized. 15 In one embodiment, a therapeutically effective amount of PYY or an agonist thereof is administered as a single pulse dose, as a bolus dose, or as pulse doses administered over time. Thus, in pulse doses, a bolus administration of PYY is provided, followed by a time period wherein no PYY is administered to the subject, followed by a second bolus administration. In specific, non-limiting examples, 20 pulse doses of PYY are administered during the course of a day, during the course of a week, or during the course of a month. The therapeutically effective amount of PYY or an agonist thereof will be dependent on the molecule utilized, the subject being treated, the severity and type of the affliction, and the manner of administration. For example, a therapeutically 25 effective amount of PYY or an agonist thereof can vary from about 0.01 sg per kilogram (kg) body weight to about 1 g per kg body weight, such as about I pg to about 5 mg per kg body weight, or about 5pg to about 1 mg per kg body weight. In another embodiment, PYY or an agonist thereof is administered to a subject at 0.5 to 135 picomole (pmol) per kg body weight, or about 72 pmol per kg body weight. 30 In one specific, non-limiting example about 5 to about 50 nmol is administered as a subcutaneous injection, such as about 2 to about 20 nmol, or about 10 nmol is administered as a subcutaneous injection. The exact dose is readily determined by -27 one of skill in the art based on the potency of the specific compound (such as the PYY polypeptide, or agonist) utilized, the age, weight, sex and physiological condition of the subject. The dose of an agonist can be a molar equivalent of the therapeutically effective dose of PYY or PYY 3-36. 5 The compositions or pharmaceutical compositions can be administered by any route, including intravenous, intraperitoneal, subcutaneous, sublingual, transdermal, intramuscular, oral, topical, transmucosal, or by pulmonary inhalation. Compositions useful in the disclosure may conveniently be provided in the form of formulations suitable for parenteral (including intravenous, intramuscular and 10 subcutaneous), nasal or oral administration. The term "parenteral" as used herein refers to modes of administration which include intravenous, intramuscular, intraperitoneal, intrasternal, subcutaneous and intraarticular injection and infusion. PYY, including PYY 3

.

36 , an agonist of PYY, or an antagonist of PYY, can be administered subcutaneously. It is well known in the art that subcutaneous 15 injections can be easily self-administered. In some cases, it will be convenient to provide a PYY or a PYY agonist and another food-intake-reducing, plasma glucose-lowering or plasma lipid-altering agent, in a single composition or solution for administration together. In other cases, it may be more advantageous to administer the additional agent separately from said 20 PYY or PYY agonist. A suitable administration format may best be determined by a medical practitioner for each patient individually. Various pharmaceutically acceptable carriers and their formulation are described in standard formulation treatises, e.g., Remington's Pharmaceutical Sciences by E. W. Martin. See also Wang, Y. J. and 25 Hanson, M. A., Journal ofParenteral Science and Technology, Technical Report No. 10, Supp. 42:2S, 1988. PYY and PYY agonists useful in the methods of this disclosure can be provided as parenteral compositions, e.g., for injection or infusion. Preferably, they are suspended in an aqueous carrier, for example, in an isotonic buffer solution at a 30 pH of about 3.0 to about 8.0, preferably at a pH of about 3.5 to about 7.4, 3.5 to 6.0, or 3.5 to about 5.0. Useful buffers include sodium citrate-citric acid and sodium phosphate-phosphoric acid, and sodium acetate/acetic acid buffers. A form of -28 repository or "depot" slow release preparation may be used so that therapeutically effective amounts of the preparation are delivered into the bloodstream over many hours or days following transdermal injection or delivery. Since the PYY and agonists are amphoteric, they may be utilized as free 5 bases, as acid addition salts or as metal salts. The salts must, of course, be pharmaceutically acceptable, and these will include metal salts, particularly alkali and alkaline earth metal salts, e.g., potassium or sodium salts. A wide variety of pharmaceutically acceptable acid addition salts are available. Such products are readily prepared by procedures well known to those skilled in the art. 10 For use by the physician, the compositions can be provided in dosage unit form containing an amount of a PYY or a PYY agonist with or without another active ingredient, e.g., a food intake-reducing, plasma glucose-lowering or plasma lipid-altering agent. Administration may begin whenever the suppression of nutrient availability, food intake, weight, blood glucose or plasma lipid lowering is desired, 15 for example, at the first sign of symptoms of a weight-related disorder or shortly after diagnosis of obesity, diabetes mellitus, or insulin resistance syndrome. Therapeutically effective amounts of a PYY or a PYY agonist for use in reducing nutrient availability are those that suppress appetite at a desired level. As will be recognized by those in the field, an effective amount of therapeutic agent will 20 vary with many factors including the potency of the particular compound, age and weight of the patient, the patient's physical condition, the blood sugar level, the weight level to be obtained, and other factors. As will be recognized by those in the field, an effective amount of this therapeutic agent will also vary with many factors including the potency of the particular compound, age and weight of the patient, the 25 patient's physical condition, the blood sugar level, the weight level to be obtained, and other factors. Administration may begin whenever the increased of nutrient availability, food intake, weight, blood glucose or plasma lipid lowering is desired, such as, but not limited to, at the first sign of symptoms of a anorexia or at the onset of weight loss due to AIDS. 30 The optimal formulation and mode of administration of PYY or a PYY agonist to a patient depend on factors known in the art such as the particular disease or disorder, the desired effect, and the type of patient. While the PYY and PYY -29 agonists will typically be used to treat human subjects they may also be used to treat similar or identical diseases in other vertebrates such as other primates, farm animals such as swine, cattle and poultry, and sport animals and pets such as horses, dogs and cats. 5 As a pharmaceutical medicament the PYY and PYY agonists of the present disclosure may be administered directly by any suitable technique, including parenterally, intranasally, orally, or by absorption through the skin. The specific route of administration of each agent will depend, e.g., on the medical history of the animal. 10 For parenteral administration, in one embodiment, PYY and PYY agonists can be formulated generaUy by mixing it at the desired degree of purity, in a unit dosage injectable form (solution, suspension, or emulsion), with a pharmaceutically acceptable carrier, i.e., one that is non-toxic to recipients at the dosages and concentrations employed and is compatible with other ingredients of the 15 formulation. "Pharmaceutically acceptable carrier" refers to a non-toxic solid, semisolid or liquid filler, diluent, encapsulating material or formulation auxiliary of any type. For example, the formulation preferably does not include oxidizing agents and other compounds that are known to be deleterious to PYY and PYY agonists. Generally, the formulations are prepared by contacting the PYY or PYY 20 agonist uniformly and intimately with liquid carriers or finely divided solid carriers or both. Then, if necessary, the product is shaped into the desired formulation. Preferably the carrier is a parenteral carrier, more preferably a solution that is isotonic with the blood of the recipient. Examples of such carrier vehicles include water, saline, Ringer's solution, and dextrose solution. Non-aqueous vehicles such 25 as fixed oils and ethyl oleate are also useful herein, as well as liposomes. PPY and PYY agonists are also suitably administered by sustained-release systems. Suitable examples of sustained-release PYY and PYY agonists include suitable polymeric materials (such as, for example, semi-permeable polymer matrices in the form of shaped articles, e.g., films, or mimcapsules), suitable 30 hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, and sparingly soluble derivatives (such as, for example, a sparingly soluble salt). Sustained-release PPY and PYY agonist compositions may be -30 administered orally, rectally, parenterally, intracistemally, intravaginally, intraperitoneally, topically (as by powders, ointments, gels, drops or transdermal patch), bucally, or as an oral or nasal spray. Sustained release matrices include polylactides (U.S. Patent No. 3,773,919, 5 EP 58,481), copolymers of L-glutamic acid and gamma-ethyl-L-glutamate (Sidman et al., Biopolymers 22:547-556, 1983, poly(2-hydroxyethyl methacrylate)); (Langer et al., J Biomed. Mater. Res. 15:167-277, 1981; Langer, Chem. Tech. 12:98-105, 1982, ethylene vinyl acetate (Langer et al., Id.) or poly-D-(-)-3-hydroxybutyric acid (EP 133,988). 10 Sustained-release PPY and PYY agonists include liposomally PPY and PYY agonists (see generally, Langer, Science 249:1527-1533, 1990; Treat et al., in Liposomes in the Therapy of Infectious Disease and Cancer, Lopez-Berestein and Fidler (eds.), Liss, New York, pp. 317-327 and 353-365, 1989). Liposomes containing PPY peptide and peptide analogs are prepared by methods known per se: 15 DE 3,218,121; Epstein et al., Proc. NatI. Acad. Sci. U.S.A. 82:3688-3692, 1985; Hwang et al., Proc. Nati. Acad. Sci. US.A. 77:4030-4034, 1980; EP 52,322; EP 36,676; EP 88,046; EP 143,949; EP 142,641; Japanese Patent Application No. 83-118008; U.S. Patent No. 4,485,045, U.S. Patent No. 4,544,545; and EP 102,324. Ordinarily, the liposomes are of the small (about 200-800 Angstroms) unilamellar 20 type in which the lipid content is greater than about 30 mole percent cholesterol, the selected proportion being adjusted for the optimal performance. Preparations for administration can be suitably formulated to give controlled release of PYY and PYY agonists. For example, the pharmaceutical compositions may be in the form of particles comprising a biodegradable polymer and/or a 25 polysaccharide jellifying and/or bioadhesive polymer, an amphiphilic polymer, an agent modifying the interface properties of the particles and a pharmacologically active substance. These compositions exhibit certain biocompatibility features which allow a controlled release of the active substance. See U.S. Patent No. 5,700,486. 30 In yet an additional embodiment, PPY and PYY agonists are delivered by way of a pump (see Langer, supra; Sefton, CRC Crit. Ref Biomed. Eng. 14:20 1, 1987; Buchwald et al., Surgery 88:507, 1980; Saudek et al., N. Engl. J. Med.

-31 321:574, 1989) or by continuous subcutaneous infusions, for example, using a mini pump. An intravenous bag solution may also be employed. The key factor in selecting an appropriate dose is the result obtained, as measured by decreases in total body weight or ratio of fat to lean mass, or by other criteria for measuring control or 5 prevention of obesity or prevention of obesity-related conditions, as are deemed appropriate by the practitioner. Other controlled release systems are discussed in the review by Langer (Science 249:1527-1533, 1990). In another aspect of the disclosure, PPY and PYY agonists are delivered by way of an implanted pump, described, for example, in U.S. Patent No. 6,436,091; 10 U.S. Patent No. 5,939,380; U.S. Patent No. 5,993,414. Implantable drug infusion devices are used to provide patients with a constant and long term dosage or infusion of a drug or any other therapeutic agent. Essentially such device may be categorized as either active or passive. Active drug or programmable infusion devices feature a pump or a metering 15 system to deliver the drug into the patient's system. An example of such an active drug infusion device currently available is the Medtronic SynchroMed T M programmable pump. Such pumps typically include a drug reservoir, a peristaltic pump to pump out the drug from the reservoir, and a catheter port to transport the pumped out drug from the reservoir via the pump to a patient's anatomy. Such 20 devices also typically include a battery to power the pump as well as an electronic module to control the flow rate of the pump. The Medtronic SynchroMedTM pump further includes an antenna to permit the remote programming of the pump. Passive drug infusion devices, in contrast, do not feature a pump, but rather rely upon a pressurized drug reservoir to deliver the drug. Thus such devices tend to be both 25 smaller as well as cheaper as compared to active devices. An example of such a device includes the Medtronic IsoMed"h. This device delivers the drug into the patient through the force provided by a pressurized reservoir applied across a flow control unit. The implanted pump can be completely implanted under the skin of a patient, 30 thereby negating the need for a percutaneous catheter. These implanted pumps can provide the patient with PYY or a PYY agonist at a constant or a programmed delivery rate, e.g., to give pulsed doses at or around meal time. Constant rate or -32 programmable rate pumps are based on either phase-change or peristaltic technology. When a constant, unchanging delivery rate is required, a constant-rate pump is well suited for long-term implanted drug delivery. If changes to the infusion rate are expected, a programmable pump may be used in place of the 5 constant rate pump system. Osmotic pumps may be much smaller than other constant rate or programmable pumps, because their infusion rate can be very low. An example of such a pump is described listed in U.S. Patent No. 5,728,396. For oral administration, the pharmaceutical compositions can take the form of, for example, tablets or capsules prepared by conventional means with 10 pharmaceutically acceptable excipients such as binding agents (e.g., pregelatinized maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g., lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (e.g., magnesium stearate, tale or silica); disintegrants (e.g., potato starch or sodium starch glycolate); or wetting agents (e.g., sodium lauryl sulphate). The tablets can be 15 coated by methods well known in the art. Liquid preparations for oral administration can take the form of, for example, solutions, syrups or suspensions, or they can be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations can be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g., sorbitol 20 syrup, cellulose derivatives or hydrogenated edible fats); emulsifying agents (e.g., lecithin or acacia); non-aqueous vehicles (e.g., almond oil, oily esters, ethyl alcohol or fractionated vegetable oils); and preservatives (e.g., methyl or propyl-p hydroxybenzoates or sorbic acid). The preparations can also contain buffer salts, flavoring, coloring and sweetening agents as appropriate. 25 For administration by inhalation, the compounds for use according to the present disclosure are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebulizer, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a 30 pressurized aerosol the dosage unit can be determined by providing a valve to deliver a metered amount. Capsules and cartridges of e.g., gelatin for use in an inhaler or insufflator can be formulated containing a powder mix of the compound -33 and a suitable powder base such as lactose or starch. The compounds can also be formulated in rectal compositions such as suppositories or retention enemas, e.g., containing conventional suppository bases such as cocoa butter or other glycerides. 5 In addition to the formulations described previously, the compounds can also be formulated as a depot preparation. Such long acting formulations can be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection. Thus, for example, the compounds can be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an 10 acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt. Pharmaceutical compositions that comprise a PYY, or an agonist thereof, as described herein as an active ingredient will normally be formulated with an appropriate solid or liquid carrier, depending upon the particular mode of 15 administration chosen. The pharmaceutically acceptable carriers and excipients useful in this disclosure are conventional. For instance, parenteral formulations usually comprise injectable fluids that are pharmaceutically and physiologically acceptable fluid vehicles such as water, physiological saline, other balanced salt solutions, aqueous dextrose, glycerol or the like. Excipients that can be included 20 are, for instance, other proteins, such as human serum albumin or plasma preparations. If desired, the pharmaceutical composition to be administered may also contain minor-amounts of non-toxic auxiliary substances, such as wetting or emulsifying agents, preservatives, and pH buffering agents and the like, for example sodium acetate or sorbitan monolaurate. Other medicinal and pharmaceutical 25 agents, for instance other appetite suppressants, or protease inhibitors, also may be included. Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in the art. The dosage form of the pharmaceutical composition will be determined by the mode of administration chosen. For instance, in addition to injectable fluids, 30 inhalation, suppository, and oral formulations can be employed. The pharmaceutical compositions can be produced of conventional mixing, granulating, confectioning, dissolving or lyophilizing processes.

-34 Oral formulations may be liquid (e.g., syrups, solutions or suspensions), or solid (e.g., powders, pills, tablets, or capsules). For example, pharmaceutical compositions for oral use can be obtained by combining the active ingredient with one or more solid carriers, optionally granulating a resulting mixture, and, if desired, 5 processing the mixture or granules, if appropriate with the addition of additional excipients, to form tablets or dragee cores. Suitable carriers include fillers, such as sugars, for example lactose, saccharose, mannitol or sorbitol, cellulose preparations and/or calcium phosphates, for example tricalcium phosphate or calcium hydrogen phosphate, also binders, such 10 as starches, for example corn, wheat, rice or potato starch, methylcellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose and/or polyvinylpyffolidone, and/or, if desired, disintegrators, such as the above-mentioned starches, also carboxymethyl starch, cross-linked polyvinylpyrrolidone, alginic acid or a salt thereof, such as sodium alginate. Additional excipients include flow 15 conditioners and lubricants, for example silicic acid, talc, stearic acid or salts thereof, such as magnesium or calcium stearate, and/or polyethylene glycol, or derivatives thereof. For parenteral administration compositions include suitable aqueous solutions of an active ingredient in water-soluble form, for example in the form of a 20 water-soluble salt, or aqueous injection suspensions that contain viscosity-altering substances, for example sodium carboxymethylcellulose, sorbitol and/or dextran, and, if desired, stabilizers. The active ingredient, optionally together with excipients, can also be in the form of a lyophilisate and can be made into a solution prior to parenteral administration by the addition of suitable solvents. Solutions 25 such as those that are used, for example, for parenteral administration can also be used as infusion solutions. For inhalation, PYY or an agonist thereof is administered as an aerosol or a dispersion in a carrier. In one specific, non-limiting example, PYY or an agonist thereof is administered as an aerosol from a conventional valve, such as, but not 30 limited to, a metered dose valve, through an aerosol adapter also known as an actuator. A suitable fluid carrier can be also included in the formulation, such as, but not limited to, air, a hydrocarbon, such as n-butane, propane, isopentane, -35 amongst others, or a propellant, such as, but not limited to a fluorocarbon. Optionally, a stabilizer is also included, and/or porous particles for deep lung delivery are included (e.g., see U.S. Patent No. 6,447,743). Compounds with poor solubility in aqueous systems require formulation by 5 using solubilizing agents such as ionic surfactants, cholates, polyethylene glycol (PEG), ethanol, or other agents which may have undesirable effects when used for inhalation. In addition, a treatment requiring successful delivery into alveoli of the lower pulmonary region may preclude from the formulation the use of certain irritants such as chlorofluorocarbons and should involve a minimum number of 10 required doses. Alternatively, to avoid such limitations, liposomes or hydrophobic particles can be used. In one embodiment, an inhalation formulation for a sustained release includes using aerosol droplet particles approximately 1-2.1 gm in size, or of less than 1 pm in size. Small particle aerosol liposomes and liposome-drug combinations for medical use have been previously described (e.g., see EP 15 87309854.5). According to the invention, a therapeutically effective amount of PYY or an agonist thereof is administered with a therapeutically effective amount of GLP-l or an agonist thereof. The PYY or agonist thereof and the GLP-1 or an agonist thereof maybe 20 administered simultaneously or substantially simultaneously, or sequentially, in any order. The PYY or agonist thereof and the GLP-1 or an agonist thereof may be administered in a single pharmaceutical composition or in separate compositions, and they may be administered by the same route or my different routes. If the PYY and the GLP-1 or an agonist thereof are to be administered in a 25 single pharmaceutical composition, that composition may be any of those described above for PYY or an agonist thereof. The composition may enable simultaneous or substantially simultaneous administration of the PYY or agonist thereof and the GLP-1 or an agonist thereof. If desired, the PYY or agonist thereof and the GLP-1 or an agonist thereof may be compartmentalized in the composition, for example, in 30 different layers of a tablet, or in different granules in a capsule. If desired, such compartmentalization may be designed to give different release properties to the two -36 components to enable delivery of the PYY or agonist component and the GLP-1 or an agonist thereof at different times, for example, sequentially. Alternatively, the PYY or agonist thereof and the GLP-1 or an agonist thereof may be formulated in separate pharmaceutical compositions, for example, 5 any of the pharmaceutical compositions described above for PYY and agonists thereof Such separate compositions may be administered simultaneously or substantially simultaneously, or they may be administered sequentially, in any order. If administered separately, whether sequentially or simultaneously (or substantially simultaneously), the PYY or agonist thereof and the GLP-1 or an 10 agonist thereof may be administered by the same route or by different routes. It is generally more convenient to administer all the active agents in a single composition. However, in some cases it may be necessary or appropriate to administer the active agents by different routes. For example, peptides are generally not stable on oral administration unless modified or formulated in a special way, so 15 must generally be administered via a non-oral route. Some agonists, for example, GLP-1 agonists, are chemical compounds that are stable when administered orally. It may be appropriate to administer OXM non-orally and the other component by a non-oral route. When PYY or an agonist thereof and GLP-1 or an agonist thereof are used in 20 the manufacture of a medicament for use in a treatment as described herein, the medicament may be a single pharmaceutical composition comprising both components, as described above, or may be a two-component medicament, one component being a pharmaceutical composition comprising PYY or an agonist thereof, the other component being a pharmaceutical composition comprising GLP-1 25 or an agonist thereof, see above. The medicament, whether a one component medicament or a two component medicament as described above, will generally be packaged with instructions relating to its use. Such instructions will refer to the timing, dose and route of administration of the component(s). If desired, a further agent that influences food intake and/or appetite may 30 also be administered. Such agents include naturally-occurring agents and agonists thereof, for example, amylin and amylin agonists, leptin and leptin agonosts, and oxyntomodulin.

-37 If desired, one or more other agents, such as, but not limited to, an additional appetite suppressant, may also be administered. Specific, non-limiting example of an additional appetite suppressant include amfepramone (diethylpropion), phentermine, mazindol and phenylpropanolamine, fenfluramine, dexfenflurainne, 5 and fluoxetine. PYY or a PYY agonist, or GLP- 1 or a GLP- 1 agonist, or both, can be administered simultaneously with the additional agent(s), or the various substances may be administered sequentially, and in any order. Thus, in one embodiment, PYY or an agonist thereof and GLP-1 or an agonist thereof is formulated and 10 administered with an additional agent, for example, oxyntomodulin or an agonist thereof, or an appetite suppressant as a single composition. Additionally, a method of treating obesity is disclosed herein. The method includes administering to an obese subject a therapeutically effective amount of PYY or a PYY agonist, and GLP-l or an agonist thereof. The PYY agonist can 15 have potency in at least one of food intake or gastric emptying greater than NPY. PYY or a PYY agonist can be administered peripherally, such as in a single or divided dose. Suitable single or divided doses include, but are not limited to, 1 sg to about 5 mg or about 0.01 trg/kg to about 500 pg/kg per dose. The subject can be insulin resistant or glucose intolerant, or both. In addition to being obese, the 20 subject can have diabetes mellitus. A method of reducing food intake is also disclosed herein. The method includes administering to an obese subject a therapeutically effective amount of PYY or a PYY agonist, and GLP-1 or an agonist thereof. The PYY agonist can have potency in at least one of food intake or gastric emptying greater than NPY. 25 PYY or a PYY agonist can be administered peripherally, such as in a single or divided dose. Suitable single or divided doses include, but are not limited to, 1 pg to about 5 mg or about 0.01 pxg/kg to about 500 pLg/kg per dose. The subject can have -Type II diabetes, and/or can be overweight. A method is disclosed herein for improving lipid profile in a subject. The 30 method includes administering to the subject an effective amount of PYY or a PYY agonist, and GLP-l or an agonist thereof. An improvement in lipid profile includes, but is not limited to, at least one method of reducing cholesterol levels, reducing -38 triglyceride levels and increasing HDL cholesterol levels. PYY or a PYY agonist can be administered peripherally, such as in a single or divided dose. PYY or a PYY agonist can be administered peripherally, such as in a single or divided dose. Suitable single or divided doses include, but are not limited to, I pg to about 5 mg or 5 about 0.01 prg/kg to about 500 pg/kg per dose. The PYY agonist can have potency in at least one of food intake or gastric emptying greater than NPY. In another embodiment, a method is disclosed herein for alleviating a condition or disorder which can be afleviated by reducing nutrient availability. The method includes administering to a subject a therapeutically effective amount of 10 PYY or a PYY agonist, and GLP-1 or an agonist thereof. Suitable disorders include any of the disorders mentioned above. The PYY or a PYY agonist and GLP-1 or GLP-1 agonist can be administered peripherally, such as in a single or divided dose. Suitable single or divided doses include, but are not limited to, 1 pg to about 5 mg or about 0.01 pg/kg 15 to about 500 pg/kg of PYY or a PYY agonist per dose. The PYY agonist can have potency in at least one of food intake or gastric emptying greater than NPY. Suitable doses also include those that raise the concentration of PYY and/or the agonist thereof significantly above the basal concentration of PYY, such as, but not limited to, a dose that that mimic postparandial serum concentrations of PYY (or the 20 agonist). Thus, in one embodiment, PYY or an agonist thereof is administered to achieve the level of to effect a reduction in calorie intake, food intake, or appetite equivalent to the reduction in calorie intake, food intake, or appetite, or to increase the energy expenditure, caused by the postprandial level of PYY 3

.

36 . Specific, non limiting examples of doses include, but are not limited doses that produce the effect 25 demonstrated when the serum levels of PYY are from about 40 pM/litre to about 60 pM/litre, for example, from about 40 pM/litre to about 50 pM/litre, or from about 40 pM/litre to about 45 pM/litre, or to about 43 pM/litre. For all methods disclosed herein, the dose of PYY or PYY 3.36 can be-based on the physiological levels observed post-prandially. The normal circulating levels 30 of PYY 3.36 are about 8 pmol/litre, typically rising to about 40 to 60 pmollitre after a meal. Agonists of PYY can be used at analogous doses. A single dose may be administered per day, or divided doses can be used (see above).

-39 As indicated above, PYY 3

-

36 has a prolonged and sustained action, continuing to act even after it has been cleared from the circulating blood, for example, for up to 12 and even up to 24 hours. Accordingly, PYY or an agonist thereof may be administered twice per day or even just once. If desired, more doses 5 may be administered, for example, three or four per day. For example, it may bc desired to administer PYY or an agonist thereof before a meal, for example, about half an hour before a meal. It may be desired to administer PYY or an agonist thereof before each meal. Alternately, it may be desired to administer as few doses as possible, in which case a regime or one or two doses per day may be used. 10 GLP-1 or an agonist may be administered according to the same regime as the PYY or agonist thereof, whether administered simultaneously or substantially simultaneously with the PYY or PYY agonist, or sequentially. Alternatively, the GLP-I may be administered in a different regime, for example, with more doses of GLP- 1 than of PYY, for example, the PYY or PYY agonist may be administered 15 once or twice per day, and the GLP-l or agonist thereof may be administered three or four times per day. For example, PYY or a PYY agonist may be administered in the morning and evening, for example, before breakfast and before the evening meal, and the GLP-1 agonist thereof may also be administered before the mid-day mcal. 20 In one embodiment, when administered peripherally, PYY, including

PYY

3

-

36 has its effects at physiological levels. No side effects are observed when PYY 3.36 is used at such levels. Without being bound by theory, PYY 3

.

36 does not affect Y2 receptors throughout the brain, which could cause side effects. It should be noted, without being limiting, that a further advantage of PYY 3

-

3 6 is that PYY 3

-

3 6 25 does not increase blood pressure. The effects of PYY 3

-

36 are as long lasting as 24 hours. Recipients claim a decrease in appetite over that period, and a reduction of food intake of about one third has been reported. - In one specific, non-limiting example, PYY3.

36 is administered in a dose of about 1 nmol or more, 2 nmol or more, or 5 nmol or more. In this example, 30 the dose of PYY 3 -36 is generally not more than 100 nmol, for example, the dose is 90 nmols or less, 80 nmols or less, 70 nmols or less, 60 nmols or less, 50 nmols or less, 40 nmols or less, 30 nmols or less, 20 nmols or less, 10 nmols. For example, a -40 dosage range may comprise any combination of any of the specified lower dose limits with any of the specified upper dose limits. Thus, exemplar non-limiting dose ranges include a dose of PYY 3

.

36 may be within the range of form I to 100 n mols, from 1 to 90 mols, from 1 to 80 nmols. Exemplary, non-limiting dose ranges 5 include, from 2 to 100 nmols, from 2 to 90 n mols, for example, from 2 to 80 nmols etc., from 5 nmols to 100 mols, from 5 nmols to 90 nmols, from 5 nmols to 80 nmols etc. By way of example, a dose of from about 5 to about 50 nmol may be administered such as, but not limited to, from about 2 to about 20 nmol, for example, about 10 nmol. The selected dose may be administered for example, by 10 injection, for example, as a subcutaneous injection. A PYY agonist may be administered at the same dose. In a further, non-limiting example, PYY 3-36 may be administered peripherally at a dose of 0.1 nmoles or more per kg body weight of the subject, for example, 0.2 nmoles or more, for example, 0.4 nmoles or more, for example, 0.6 15 moles or more, for example, 0.8 nmoles or more, for example, 1.0 nmole or more, for example, 1.2 moles or more, for example, 1.4 moles or more, for example, 1.6 nmoles or more, for example, 1.8 moles or more, for example, 2.0 nmoles or more, for example, 2.2 moles or more, for example, 2.4 moles or more, for example, 2.6 nmoles or more, for example, 2.8 nmoles, for example, 3.0 nmoles or more, for 20 example, up to 3.2 nmoles per kg body weight PYY 3.36 or a PYY agonist andmay be administered peripherally in an amount of up to 3.0 nmoles per kg body weight, for example, up to 2.8 nmoles, for example, up to 2.6 nmoles, for example, up to 2.4 nmoles, for example, up to 2.2 nmoles, for example, up to 2.0 nmoles, for example, up to 1.8 moles, for example, up to 1.4 moles, for example, up to 1.2 moles, for 25 example, up to 1.0 moles, for example, up to 0.8 moles, for example, up to 0.6 nmoles, for example, up to 0.4 moles, for example, up to 0.2 nmoles per kg body weight. For subcutaneous administration, a dose of PYY for example PYY 3

-

36 or a PYY agonist of 10 nmoles or more, for example, 20 nmoles or more, for example, 30 30 nmoles or more, for example, 40 nmoles or more may be used. It is generally preferable to use up to about 70 nmoles, for example, up to about 60 namoles. A dose of about 40nmoles may be used. In one embodiment, a dose of PYY or PYY 3

.

-41 36 at 0.143 n moles (1/7h of a mole) is administered per kilogram, to achieve a dose that is similar to the postprandial level of PYY. If PYY itself or an agonist thereof is used, the dose is preferably a molar equivalent of a PYY 3

.

3 6 dose, or achieves the same effect as a PYY 336 dose, as 5 described above. The doses can be calculated on the basis of a subject, such as a subject weighing from 70 to 75 kg. The exact dose is readily determined by one of skill in the art based on the potency of the specific compound (such as the PYY polypeptide, or agonist) utilized, and the age, weight, sex and physiological condition of the subject. 10 All the teachings given above in relation to PYY 3

.

36 also apply to PYY itself and to PYY agonists. For example, the teachings relating to routes of administration and dosage regimes. The GLP-1 or an agonist thereof should be administered in an therapeutically effective dose, see the section relating to GLP-l and agonists thereof below. GLP-1 15 or an agonist thereof may be used in amounts or doses in the ranges described above for PYY or a PYY agonist. The doses of the various agent may be independent of each other or, for example, equimolar doses may be used, for example, equimolar doses of GLP-1 or an agonist thereof and PYY or an agonist thereof. As disclosed herein, a naturally occurring peptide, PYY or PYY 3

.

36 can be 20 used to achieve a physiological effect. This results in minimal side effects and enables long term use, if necessary. The dose of PYY or PYY 3

.

36 can be based on the physiological levels observed post-prandially. The normal circulating levels of

PYY

3

-

36 are about 8 pmol/litre, typically rising to about 40 to 60 pmol/litre after a meal. PYY (e.g., PYY 3 -36) and agonists can be used at analogous doses. 25 The considerations set out above in relation to the administration regime also relate to administration using the subcutaneous route. The various uses of PYY, or an agonist thereof, and GLP-1 or an agonist thereof, as set out above may be in a method of treatment of a mammalian subject in need of such treatment, or may be in the manufacture of a medicament for such 30 treatment. PYY (e.g., PYY 3

.

36 ) or an agonist thereof, and GLP-1 or an agonist thereof, should be administered in an amount effective to achieve the stated object. Some of the treatments described above are medical treatments, for example, the -42 treatment of obesity. Others, however, do not relate to medical treatment, and are part of the maintenance of a healthy lifestyle, or are for cosmetic purposes. The use of a combination of any of OXM and GLP-1 or an agonist thereof and PYY or an agonist thereof may serve to increase the effectiveness of any of the 5 agents compared with its use alone, for example, as described above. Alternatively or in addition, use of the two or three agents in combination may reduce any tendency for "escape" when using an agent alone. The term "escape" is used to denote a reduction in effect of an agent with time. For example, if any one of the agents above has been used alone, its effect may reduce with time. Use of one or 10 both of the other agents in addition may reduce or prevent the tendency for that reduction in effectiveness. For example, PYY has a sustained effect and may be used for prolonged periods. If the effect of PYY should appear to reduce, or to reduce or prevent any such reduction in effect, OXM may be administered in addition to the PYY. GLP-1 may also be used for the same purpose, with OXM or 15 with OXM and PYY. In another aspect the present invention provides a pharmaceutical composition that comprises PYY or a PYY agonist as the active ingredient and a pharmaceutically suitable carrier in a form suitable for subcutaneous administration, for example, as an injectable solution. Such a composition may comprise an amount 20 of PYY for example PYY 3

.

3 6 or a PYY agonist of 10 moles or more, for example, 20 nmoles or more, for example, 30 moles or more, for example, 40 moles or more may be used. It is generally preferable to use up to about 70 moles, for example, up to about 60 nnmoles. An amount of about 40nmoles may present.. Suitable formulations for subcutaneous administration are well known, and are 25 described above. One or more further active ingredient(s) may also be present in the composition, in particular GLP-1 or an agonist thereof, which may be used in amounts and doses as described above. Further active ingredients may be present, for example, as described above, for example, oxyntomodulin or an agonist thereof, or an appetite suppressant as described above. 30 A further aspect of the invention relates to methods as disclosed above, including a method for decreasing calorie intake in a subject, a method for decreasing appetite in a subject, a method for decreasing food intake in a subject, a -43 method for weight control or treatment in a subject, and a method for reduction or prevention of obesity, in particular any one or more of the following: preventing and reducing weight gain; inducing and promoting weight loss; and reducing obesity as measured by the Body Mass Index. The methods include control of any one or more 5 of appetite, satiety and hunger, in particular any one or more of the following: reducing, suppressing and inhibiting appetite; inducing, increasing, enhancing and promoting satiety and sensations of satiety; and reducing, inhibiting and suppressing hunger and sensations of hunger. The methods further include maintaining any one or more of a desired body weight, a desired Body Mass Index, a desired appearance 10 and good health. In the methods of this aspect of the invention, PYY, for example,

PYY

3 .a 6 or a PYY agonist is administered subcutaneously to the subject. Preferably the PYY or PYY agonist is administered at dose of 10 nmoles or more, for example, 20 nmoles or more, for example, 30 moles or more, for example, 40 moles or more may be used. It is generally preferable to use up to 15 about 70 moles, for example, up to about 60 nnmoles. An dose of about 40nmoles be used. As indicated above, PYY 3

.

36 has a prolonged and sustained action, continuing to act even after it has been cleared from the circulating blood, for example, for up to 12 and even up to 24 hours. Accordingly, PYY or an agonist thereof may be administered twice per day or even just once. If desired, more doses 20 may be administered, for example, three or four per day. For example, it may be desired to administer PYY or an agonist thereof before a meal, for example, about half an hour before a meal. It may be desired to administer PYY or an agonist thereof before each meal. Alternately, it may be desired to administer as few doses as possible, in which case a regime or one or two doses per day may be used. 25 In this aspect of the invention, PYY or an agonist thereof may be administered alone or PYY or an agonist thereof and GLP-1 or an agonist thereof may be administered. If GLP-1 or an agonist thereof is used in addition to the PYY or PYY agonist, the GLP-1 or agonist thereof may be administered simultaneously or substantially simultaneously with the PYY or agonist thereof, or sequentially, in 30 either order. The PYY or agonist thereof and the GLP-1 or agonist thereof may be administered in a single pharmaceutical composition or in separate compositions, and they may be administered by the same route or by different routes. Further -44 active ingredients may also be administered, for example, as described above for subcutaneous PYY pharmaceutical compositions. The present invention also includes PYY or an agonist thereof for use in the manufacture of a medicament for subcutaneous administration for any of the 5 methods of treatment described above. PYY Agonists A PYY agonist, of use in the methods of the present disclosure, is a molecule 10 that binds to a receptor that specifically binds PYY, and elicits an effect of PYY. Assays for binding to PYY receptors, and eliciting a response in a cell with a PYY receptor, are known in the art. A specific assay for detecting a PYY agonist is also disclosed herein. Thus, in one embodiment, a PYY agonist binds to a NPY neuron in the arcuate nucleus, which results in an electrophysiological effect on an NPY 15 neuron. As disclosed herein, NPY neurons synapse with POMC neurons. Thus, the electrophysiological effect on the NYP neuron can result in a further electrophysiological effect on a POMC neuron. In one specific, non-limiting example, an administration of PYY agonist results in hyperpolization of the membrane potential of a POMC neuron. In another specific, non-limiting example, 20 administration of a PYY agonist results in an increase in IPSCs in a POMC neuron. In another embodiment, PYY agonists do not include NPY. Suitable PYY agonists include molecules that bind NPY neurons, but do not cross the blood/brain barrier. The arcuate nucleus neurons upon which PYY exerts its effects are not protected by the blood/brain barrier, and thus are readily accessible to peripherally 25 available molecules. In addition, other brain sites that express the Y2 receptor are protected by the blood/brain barrier. Without being bound by theory, agents able to bind to the arcuate Y2R, but that do not cross the blood/brain barrier following peripheral administration, are likely to be of use. In one embodiment, a PYY agonist is a compound that affects food intake, 30 caloric intake, or appetite, and/or which binds specifically in a Y receptor assay or competes for binding with PYY, such as in a competitive binding assay with labeled -45 PYY. PYY agonists include, but are not limited to, compounds that bind to the Y2 receptor. PYY and agonists useful in the methods disclosed herein include, but are not limited to, polypeptides comprising, or alternatively consisting of, the amino acid 5 sequence for PPY and agonists thereof, e.g., mutants, fragments and/or variants thereof. Variants include deletions, insertions, inversions, repeats and substitutions (e.g., conservative substitutions and non-conservative substitutions; see, e.g., Tables 1 and 2, infra). More than one amino acid (e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, etc.) can be deleted or inserted or substituted with another amino acid. Typically conservative 10 substitutions are the replacements, one for another, among the aliphatic amino acids Ala, Val, Leu and Ile; interchange of Ser and Thr containing hydroxy residues, interchange of the acidic residues Asp and Glu, interchange between the amide residues Asn and Gin, interchange of the basic residues Lys and Arg, interchange of the aromatic residues Phe and Tyr, and interchange of the small-sized amino acids 15 Ala, Ser, Thr, Met and Gly. Guidance concerning how to make phenotypically silent amino acid substitutions is provided in Bowie et al., Science 247:1306-1310, 1990. As another example, polypeptide fragments may contain a continuous series of deleted residues from the amino (N)- or the carboxyl (C)- terminus, or both (see, 20 e.g., Tables 1 and 2, infra). Any number of amino acids, ranging from 1 to 24, can be deleted from the N-terminus, the C-terminus or both. Furthermore, the agonist polypeptides may also include, but are not limited to, polypeptides comprising, or alternatively consisting of, internal deletions of the amino acid sequences for PPY and/or agonist thereof (see, e.g., Table 2, infra). 25 Such deletions may comprise one or more amino acid residue deletions (e.g., one, two, three, four, five, six, seven, eight, nine, ten, etc.) and may begin at any amino acid position (e.g., two, three, four, five, six, seven, eight, nine, ten, etc.). In addition, the polypeptides-of this disclosure may contain one or more such internal deletions. Such deletions are contemplated in PPY, NPY and PP. 30 Also contemplated are agonist peptides that are PPY, NPY and/or PP chimeras having high affinity and/or selectivity for the Y2 receptor. These chimeras may comprise amino acid substitutions of one or more amino acids (e.g., 1, 2, 3, 4, -46 5, 6, 7, 8, 9, 10, etc.) from PPY, NPY and/or PP, variants, mutants and/or deletions thereof, with one or more amino acids (e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, etc.) from a second PPY, NPY, or PP, variants, mutations and/or deletions thereof. These substitutions may begin at any amino acid position (e.g., two, three, four, five, six, 5 seven, cight, nine, ten, etc.). Preferably, the peptide is selective for the Y2 receptor. That is, it binds with higher affinity to Y2 compared to other receptors, such as Yl, Y2, Y3, Y4, Y5 and Y6. In another embodiment, the peptide is selective for the Y2 and Y5 receptors over the YI, Y3, Y4 and Y6 receptors. 10 Other polypeptide fragments are fragments comprising structural or functional domain of the polypeptides of this disclosure. Such fragments include amino acid residues that comprise a polyproline-type II helix (residues 1-8), beta turn (residues 9-14), amphipathic alpha-helix (residues 15-32) and/or a C-terminal turn structure (residues 33-36). See, Kirby et al., JMed Chem 36:385-393, 1993. 15 In addition, this disclosure includes the use of a polypeptide or agonist comprising, or alternatively consisting of, the amino acid sequence for PPY, NPY and PP species variants (see Table 1, infra) and/or mutants, and fragments thereof Also contemplated are fusion proteins, whereby a PYY or PYY agonist will be fused to another protein or polypeptide (the fusion partner) using recombinant 20 methods known in the art. Alternatively, such a fusion protein may be synthetically synthesized by any known method. Any known peptide or protein can be used as the fusion partner (e.g., serum albumin, carbonic anhydrase, glutathione-S transferase or thioredoxin, etc.). Preferred fusion partners will not have an adverse biological activity in vivo. Such fusion proteins may be designed linking the 25 carboxy-terminus of the fusion partner to the amino-terminus of the PYY or agonist peptide, or vice versa. Optionally, a cleavable linker region may be used linking the PYY or PYY agonist to the fusion partner, and may be cleaved in vivo thereby resulting in the release of an active form of PYY or a PYY agonist. Examples of such cleavage regions include, but are not limited to, the linker regions D-D-D-D-Y 30 (SEQ ID NO: 330), G-P-R (SEQ ID NO: 331), A-G-G (SEQ ID NO: 332) and H-P F-H-L (SEQ ID NO 333), which can be cleaved by enterokinase, thrombin, ubiquitin cleaving enzyme and renin, respectfully. See, e.g., U.S. Patent No. 6,410,707.

-47 Also contemplated as useful PYY agonists are Y2 specific NPY peptide agonists as described in U.S. Patent No. 5,026,685; U.S. Patent No. 5,574,010; U.S. Patent No. 5,604,203; U.S. Patent No. 5,696,093; U.S. Patent No. 6,046,167. See below: 5 Preferred PPY agonists are described herein as follows. TABLE 1 - PYY: Variation Among Species 10 PEPTIDE YY AA SEQUENCE Human YPIKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 1) Rat YPAKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY (SEQ ID NO: 5) Pig YPAKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY (SEQ ID NO: 6) Guinea pig YPSKPEAPGSDASPEELARYYASLRHYLNLVTRQRY (SEQ ID NO: 7) 15 Frog YPPKPENPGEDASPEEMTKYLTALRHYINLVTRQRY (SEQ ID NO: 8) Raja YPPKPENPGDDAAPEELAKYYSALRHYINUTRQRY (SEQ ID NO: 9) Dogfish YPPKPENPGEDAPPEELAKYYSALRHYINLITRQRY (SEQ ID NO; 10) Lampetra FPPKPDNPGDNASPEQMARYKAAVRHYINUTRQRY (SEQ ID NO: 11) Petromyzon MPPKPDNPSPDASPEELSKYMLAVRNYINLTRQRY (SEQ ID NO: 12) 20 NEUROPEPTIDE Y AA SEQUENCE Human YPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRY (SEQ ID NO: 2) Rat YPSKPDNPGEDAPAEDMARYYSALRHYINLTRQRY (SEQ ID NO: 13) Rabbit YPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRY (SEQ ID NO: 14) 25 Dog YPSKPDNPGEDAPAEDMARYYSALRHYINLTRQRY (SEQ ID NO: 15) Pig YPSKPDNPGEDAPABDLARYYSALRHYINLITRQRY (SEQ II) NO: 16) Cow YPSKPDNPGEDAPAEDLARYYSALRHYINLITRQRY (SEQ ID NO: 17) Sheep YPSKPDNPGDDAPAEDLARYYSALRHYINLITRQRY (SEQ ID NO: 18) Guinea pig YPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRY (SEQ ID NO: 19) 30 Avian YPSKPDSPGEDAPAEDMARYYSALRHYINLITRQRY (SEQ ID NO: 20) Rana YPSKPDNPGEDAPAEDMAKYYSALRHYINLITRQRY (SEQ ID NO: 21) Goldfish YPTKPDNPGEDAPAEELAKYYSALRHYINLITRQRY (SEQ ID NO: 22) Dogfish YPSKPDNPGEGAPAEDLAKYYSALRHYINLITRQRY (SEQ ID NO: 23) Lampetra PPNKPDSPGEDAPAEDLARYLSAVRHYINLITRQRY (SEQ ID NO: 24) 35 PANCREATIC POLYPEPTIDE AA SEQUENCE Human ASLEPEYPGDNATPEQMAQYAAELRRYINMLTRPRY (SEQ ID NO: 3) -48 Sheep APLEPVYPGDNATPEQMAQYAADLRRYINMLTRPRY (SEQ ID NO: 25) Pig APLEPVYPGDDATPEQMAQYAAELRRYINMLTRPRY (SEQ ID NO: 26) Dog APLEPVYPGDDATPEQMAQYAAELRRYINMLTRPRY (SEQ ID NO: 27) Cat APLEPVYPGDNATPEQMAQYAAELRRYINMLTRPRY (SEQ D NO: 28) 5 Cow APLEPEYPGDNATPEQMAQYAAELRRYINMLTRPRY (SEQ ID NO: 29) Rat APLEPMYPGDYATHEQRAQYETQLRRYINTLTRPRY (SEQ ID NO: 30) Mouse APLEPMYPGDYATPEQMAQYETQLRRYINTLTRPRY (SEQ ID NO: 31) Guinea pig APLEPVYPGDNATPEQQMAQYAAEMRRYINMLTRPRY (SEQ ID NO: 32) Chicken GPSQPTYPGDDAPVEDLIRFYNDLQQYLNVVTRHRY (SEQ ID NO: 33) 10 Alligator TPLQPKYPGDGAPVEDLIQFYNDLQQYLNVVTRPRF (SEQ ID NO: 34) Bullfrog APSEPHHPGDQATPDQLAQYYSDLYQYITFITRPRF (SEQ ID NO: 35) Ref: Beck-Sickinger, A.G., Jung, G., Biopolymers 37:123-142, 1995. 15 TABLE 2 - PEPTIDE AGON!ST OF PYY PEPTIDE SEQUENCE PPY(3-36)(human) IKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 334) 20 Ref: Eberlein et al., Peptides 10:797-803, 1989; Grandt et al., Peptides 15(5):815 20, 1994. Variations of PPY(3-36) N-Terminal Deletions of PYY, including but not limited to: PYY(26-36), PYY(25 25 36), PYY(24-36), PYY(23-36), PYY(22-36), PYY(21-36), PYY(20-36), PYY(19 36), PYY(18-36), PYY(17-36), PYY(16-36), PYY(15-36), PYY(14-36), PYY(13 36), PYY(12-36), PYY(1 1-36), PYY(10-36), PYY(9-36), PYY(8-36), PYY(7-36), PYY(6-36), PYY(5-36), PYY(4-36), PYY(3-36). Ref: See, e.g., Balasubramaniam et al., Pept Res l(1):32-5, Sep-Oct 1998; Liu et al., 30 JGastrointest Surg 5(2):147-52, Mar-Apr 2001. PEPTIDE SEQUENCE NPY (human) YPSKPDNPGBDAPAEDMARYYSALRHYINLITRQRY (SEQ ID NO: 2) -49 Ref: Tatemoto et al., Proc Natl A cad Sci U.S.A. 79:5485-9, 1982. Variations of NPY N-Terminal Deletions of NPY, including but not limited to: NPY(26-36), NPY(25 5 36), NPY(24-36), NPY(23-36), NPY(22-36), NPY(21-36), NPY(20-36), NPY(19 36), NPY(18-36), NPY(17-36), NPY(16-36), NPY(15-36), NPY(14-36), NPY(13 36), NPY(12-36), NPY(11-36), NPY(10-36), NPY(9-36), NPY(8-36), NPY(7-36), NPY(6-36), NPY(5-36), NPY(4-36), NPY(3-36). Ref: See e.g., Gehlert et al., Proc Soc Exp Biol Med 218:7-22, 1998; Sheikh et al., 10 Am JPhysiol 261:G701-15, Nov. 1991. Internal Deletions, including but not limited to: (1-4)-Aca-(14-36)pNPY, (1-4)-Aca (15-36)pNPY, (1-4)-Aca-(16-36)pNPY, (1-4)-Aca-(17-36)pNPY, (1-4)-Aca-(18 36)pNPY, (1-4)-(31-36)pNPYl 1, (1-4)-Aca-(31-36)pNPY, (4-1)-(31-36)pNPY, (4 15 1)-Aca-(31-36)pNPY, (4-1)D-( 3 1-36)pNPY, (4-1)D-Aca-(31-36)pNPY. Ref: Fournier et al., Mol Pharmacol 45(l):93-101, Jan 1994. Additional Internal Deletion Mutants, including but not limited to: des-AA' 0 ~ NPY, des-AA' 0 1 ", Ac-[D-Lys 9 (e-Ac-Ala)]NPY, des-AA 1 0

-

7 , Ac[D-Lys 9 (E-Ac 20 Ala)]NPY, des-AAlcO~[Ala 7 ,2]NPY, des-AAo-r[Cys7 21 ]NPY, des-AA 0 ~ 17 [Glu 7 ,Lys"]NPY, des-AA' ~ 17 [D-Lys' 0 (E-Ac), Cys 7 ',2]NPY, des-AA' 0 17 [D-Cys 7 , D-Lys(e-Ac), Cys 2 ']NPY,des-AAc4- 7 [D-Cys 7 , Lys 9 (e-Ac), Cys 2 1 ]NPY, des-AA"~ 7 [Cys 7

,

2 1 , Pro 34 ]NPY, des-AAl"- 17 [Asp 7 , Dpr 2t , Pro 3 4 ]NPY, des-AA'' 7 [Glu 7 , Lys 2 1 , Pro 34 ]NPY, des-AA"- 7 [Cys 7 2 1 , Leu 31 , Pro]NPY, des-AA 1 0-2 0 {Cys 7 ', Pro]NPY, 25 des-AA'" 0 7 [Cys 2

,

2 7 ]NPY, des-AA'- 17 [Cys 2 , D-Cys 27 ]NPY. Ref: Kirby et al., JMed Chem 38:4579-86, 1995. Cyclic agonist of NPY, including burnot limited to: [Lys 25-Glu 29]NPY(Ac-25 36), [Glu 25-Lys 29]NPY(Ac-25-36), [Lys 26-Glu31]NPY(Ac-25-36), [Glu 27-Lys 30 31]NPY(Ac-25-36), [Lys28-Glu 32]NPY(Ac-25-36), [Lys27-Glu34]NPY(Ac-25 36). Ref: Ristetal.,EurJBiochem247:1019-1028, 1997.

-50 D-amnino acid substitutions: [D-Tyr']NPY, [D-Pro']NPY, [D-SerINPY, [D Lys 4 ]NPY, [D-Pro']NPY, [D-Asp 6 ]NPY, [D-Asn 7 ]NPY, [D-Pro']NPY, [D Ala 9 ]NPY, [D-Glu' 0 ]NY, [D-Asp" ]NPY, [D-Ala"]INPY, [D-Pro' 3 ]NPY, [D 5 Ala 1 4 ]Npy, [D-Glu' 5 ]NPY, [D-Asp' 6 ]NPY, [D-Leu 1 7 ]Npy, [DAla' 8 ]NPy, [D Arg' 9 ]NPY, [D-Tyr 20 ]NPY, [D-Tyr 21 ]NPY, [D-Ser 22 ]NPY, [D-Aja 23 ]NPY, [D Leu 24 ]Npy, [D-Arg 25 ]NPY, [D-His 26 INPY, [D-Tyr]NPY, [D-fle 2 ]NPY, [D-. Asn 29 ]NPY, [D-Leu 30 ]NPY, [D-I1e 3 ]NPY, [D-Thr 32 ]NPY, [D-Arg 33 ]NPY, [D Gln 3 4 ]NPY, [D-Arg 35 ]NPY, [D-Tyr 36 ]NPY, [D-Tyr', D-pro 2 ]Npy, [D-S&r, D 10 Lys 4 ]NPY, [D-Pro', D-ASp 6 ]Npy, [D-Asn 7 , D-Pro']NPY, [D-Glu' 0 , D-Asp" ]NPY, [D-Asp", D-AlaI 2 ]Npy, [D-Pro' 3 , D-A~a 14 ]Npy, (D-Glu' 5 , D-Asp' 6 ]NPY, [D Met1 7 , D-Ala' 8 ]NPY, [D-Arg' 9 , D-Tyr 20 ]NPY, [D-Tyr 2 l, D-Ser'2]NPY, [D-Ala 23 , D LeU 24 iINpy, [D-Arg", DHis1 6 ]Npy, [D-T yr", D-11e 2 Npy, [D-Asn 29 , D Leu 30 ]NPY, [D-11e 3 , D-Thr& 2 ]Npy, [D-Arg" 3 , D-G3ln 34 ]NPY, [D-Arg 35 , D 15 Tyrn 6 NPY. Ref. Kirby et al., JMed Chem 36:3802-08, 1993; Gmundemnar et al., Regulatory Peptides 62:13 1-136, 1996. Other NPY Ap-onist and Analogs 20 PEPTIDE SEQUENCE NPY(3-36) SKPDNPGEDAPAEDMARYYSALRHYINLTRQRY (SEQ ID NO: 335) Ref: Grandt et al., Regulatory Peptides 67(l):33-7, 1996. 25 PEPTIDE SEQUENCE N-Acetyl NPY(24-36) LRHYINLITRQRY (SEQ ID NO: 213) Ref: Patter et al., EurJPhamacol 267(3):253-262, May 17,1994. 30 PEPTIDE SEQUENCE N-Acetyl [Leu 28 , Leu 31 ] NPY(24-36) LRHYLNLLTRQRY (SEQ ID NO: 214) Ref: Potter et al., EurJPhamacol 267(3):253-262, May 17, 1994.

-51 PEPTIDE SEQUENCE [Leu", Leu"] NPY(24-36) LRHYLNLLTRQRY (SEQ ID NO: 215) 5 Ref: Potter et al.,EurJPharmacol267(3):253-262, May 17, 1994. PEPTIDE SEQUENCE [Leu1 7 , GIn' 9 , Ala 2 1 , Ala 22 , Glu", Leu2, Leu"] NPY(13-36) PAEDLAQYAAELRHYLNLLTRQRY (SEQ ID NO: 216) 10 Ref: Potter et al., Eur J Pharmacol 267(3):253-262, May 17, 1994. PEPTIDE SEQUENCE Cyclo S-S [Cys 20 ,Cys 24 ]pNPY SKPDNPGEDAPAEDMARCYSACRHYINLITRQRY (SEQ ID NO: 315) 15 Ref: Sol] et al., EurJBiochem 268(10):2828-37, May 2001. PEPTIDE SEQUENCE Cyclo-(28/32)-Ac-[Lys 2 -Glu 32 ]-(25-36)-pNPY RHYLNLIGRQRY (SEQ ID NO: 316) 20 Ref: Cabrele et al., JPept Sci 6(3):97-122, Mar 2000. PEPTIDE SEQUENCE Cyclo-(27/31)-Ac-[Gu 27 -Lys3 t ]-(25-36)-pNPY RHOLNLLGRQRY (SEQ ID NO: 317) 25 Ref: Cabrele et al., JPept Sci 6(3):97-122, Mar 2000. PEPTIDE SEQUENCE [Tyr", Leu3]NPY(27-36) YINLIYRLRY (SEQ ID NO: 318) 30 Ref: Leban et al.,JMed Chem 38:1150-57, 1995. PEPTIDE SEQUENCE [Tr 2 Leu 3t ]NPY(26-36) HYINLIYRLRY (SEQ ID NO: 319) 35 Ref: Leban et al., JMed Chem 38:1150-57, 1995.

-52 PEPTIDE SEQUENCE [Tyr", Leu"]NPY(25-36) RHYINLIYRLRY (SED ID NO: 320) Ref: Leban et al., JMed Chem 38:1150-57, 1995. 5 [Leu 3 ]NPY(27-36) YINLLYRQRY (SEQ ID NO: 321) Ref: Leban et al., JMed Chem 38:1150-57, 1995. 10 PEPTIDE SEQUENCE [T', Leu-] (1-4)-Ahr-(27-36)NPY YPSL-Aba-YINLIYRLRY (SED ID NO: 322) Ref: Leban et al., JMed Chem 38:1150-57, 1995. 15 PEPTIDE SEQUENCE [TyrI 2 , Leu 4 ]NPY(28-36) INLIYRLRY (SEQ ID NO: 323) Ref: Leban et al., JMed Chem 38:1150-57, 1995. 20 PEPTIDE SEQUENCE PP (human) ASLEPEYPGDNATPEQMAQYAAELRRYINMLTRPRY (SEQ ID NO: 3) Ref: Kimmel et al., Endocrinology 83:1323-30, 1968. 25 Variations of PP N-Terminal Deletions including but not limited to: PP(26-36), PP(25-36), PP(24 36), PP(23-36), PP(22-36), PP(21-36), PP(20-36), PP(19-36), PP(18-36), PP(17-36), PP(16-36), PP(15-36), PP(14-36), PP(13-36), PP(12-36), PP(1 1-36), PP(10-36), PP(9-36), PP(8-36), PP(7-36), PP(6-36), PP(5-36), PP(4-36), PP(3-36). 30 TABLE 3 - EXAMPLES OF CONSERVATIVE AMNO ACID SUBSTITUTIONS OF PYY Single point mutations of PYY(25-36) -53 PEPTIDE SEQUENCE [Lys"]PPY(25-36) KHYLNLVTRQRY (SEQ ID NO: 36) [Thr]PPY(25-36) RHTLNLVTRQRY (SEQ ID NO: 37) [Phe"]PPY(25-36) RHFLNLVTRQRY (SEQ ID NO: 38) 5 [Ilem]PYY (25-36) RHYINLVTRQRY (SEQ ID NO: 39) (Val"]PYY (25-36) RHYVNLVTRQRY (SEQ ID NO: 40) [GInI]PYY (25-36) RHYLQLVTRQRY (SEQ ID NO: 41) [le"]PYY (25-36) RHYLNIVTRQRY (SEQ ID NO: 42) [Valo]PYY (25-36) RHYLNVVTRQRY (SEQ ID NO: 43) 10 [Ile"]PYY (25-36) RHYLNLITRQRY (SEQ ID NO: 44) [Leu 3 ]PYY (25-36) RHYLNLLTRQRY (SEQ ID NO: 45) [Ser 2 ]PYY (25-36) RHYLNLVSRQRY (SEQ ID NO: 46) [Lys"]PYY (25-36) RHYLNLVTKQRY (SEQ ID NO: 47) [Asn"]PYY (25-36) RHYLNLVTRNRY (SEQ ID NO: 48) 15 [Lys"]PYY (25-36) RHYLNLVTRQKY (SEQ ID NO: 49) [Thr"]PYY (25-36) RHYLNLVTRQRT (SEQ ID NO: 50) [Phe 3 ]PYY (25-36) RHYLNLVTRQRF (SEQ ID NO: 51) Double point mutations 20 PEPTIDE SEQUENCE [Lys, Thr"]PPY(25-36) KHTLNLVTRQRY (SEQ ID NO: 52) [Lys", Phe 2 ]PPY(25-36) KHFLNLVTRQRY (SEQ ID NO: 53) [Lyse fe 2 ]PPY(25-36) KHYINLVTRQRY (SEQ ID NO: 54) [Lys", Val"]PPY(25-36) KHYVNLVTRQRY (SEQ ID NO: 55) 25 [Lyse, Gln"]PPY(25-36) KHYLQLVTRQRY (SEQ ID NO: 56) (Lyse, Ilem]PPY(25-36) KHYLNIVTRQRY (SEQ ID NO: 57) [Lys", ValP]PPY(25-36) KHYLNVVTRQRY (SEQ ID NO: 58) [Lys", Ile"]PPY(25-36) KHYLNLITRQRY (SEQ ID NO: 59) [Lys', Leu 3 ]PPY(25-36) KHYLNLLTRQRY (SEQ ID NO: 60) 30 [Lys 1, Ser 3 2 ]PPY(25-36) KHYLNLVSRQRY (SEQ ID NO: 61) [Lyse, Lys"3]PPY(25-36) KHYLNLVTKQRY (SEQ ID NO: 62) [Lys", Asnm]PPY(25-36) KHYLNLVTRNRY (SEQ ID NO: 63) [Lys", Lys"]PPY(25-36) KHYLNLVTRQKY (SEQ ID NO: 64) [Lys", Thr?]PPY(25-36) KHYLNLVTRQRT (SEQ ID NO: 65) 35 [Lys", Phey]PPY(25-36) KHYLNLVTRQRF (SEQ ID NO: 66) [Thr, Ile"]PPY(25-36) RHTINLVTRQRY (SEQ ID NO: 67) [Thr", Val"]PPY(25-36) RHTVNLVTRQRY (SEQ ID NO: 68) [Thr", Gn 2 ]PPY(25-36) RHTLQLVTRQRY (SEQ ID NO: 69) -54 [Thr7, Ile"]PPY(25-36) RHTLNIVTRQRY (SEQ ID NO: 70) [Th 2 7, Val"]PPY(25-36) RHTLNVVTRQRY (SEQ ID NO: 71) [ThrA7, Ile"]PPY(25-36) RHTLNLITRQRY (SEQ ID NO: 72) [Thr 7 , Leu"]PPY(25-36) RHTLNLLTRQRY (SEQ ID NO: 73) 5 [Thr", Se 2 ]PPY(25-36) RHTLNLVSRQRY (SEQ ID NO: 74) [Thr2, Lys"IPPY(25-36) RHTLNLVTKQRY (SEQ ID NO: 75) [Thr", Asn 34 ]PPY(25-36) RHTLNLVTRNRY (SEQ ID NO: 76) [Thr 7 , Lys']PPY(25-36) RHTLNLVTRQKY (SEQ ID NO: 77) [Thr", Th/i"PPY(25-36) RHTLNLVTRQRT (SEQ ID NO: 78) 10 (Thr", Phe"]PPY(25-36) RHTLNLVTRQRF (SEQ ID NO: 79) [phe 7 , Ile"]PPY(25-36) RHFINLVTRQRY (SEQ ID NO: 80) [Phe", Val"]PPY(25-36) RHFVNLVTRQRY (SEQ ID NO: 81) [Phe 2 , Gn 2 ]PPY(25-36) RHFLQLVTRQRY (SEQ ID NO: 82) [Phe", lle]PPY(25-36) RHFLNIVTRQRY (SEQ ID NO: 83) 15 [Phe 27 , Va1 3 ]PPY(25-36) RHFLNVVTRQRY (SEQ ID NO: 84) [Phe", Ile"]PPY(25-36) RHFLNUTRQRY (SEQ ID NO: 85) [Phe 27 , Leu 3 ]PPY(25-36) RHFLNLLTRQRY (SEQ ID NO: 86) [Phe", Ser 2 ]PPY(25-36) RHFLNLVSRQRY (SEQ ID NO: 87) [Phe 2 , Lys 3 3 ]PPY(25-36) RHFLNLVTKQRY (SEQ ID NO: 88) 20 [Phe 27 , Asnm]PPY(25-36) RHFLNLVTRNRY (SEQ ID NO: 89) [Phel, Lys"]PPY(25-36) RHFLNLVTRQKY (SEQ ID NO: 90) [Phe 27 , Thr]'JPPY(25-36) RHFLNLVTRQRT (SEQ ID NO: 91) phe 7 , Phe 3 ]PPY(25-36) RIFLNLVTRQRF (SEQ ID NO: 92) [Gin", Ile 3 ]PYY (25-36) RHYLQIVTRQRY (SEQ ID NO: 93) 25 [Gli, Val 3 ]PYY (25-36) RHYLQVVTRQRY (SEQ ID NO: 94) [Gn 2 , lIe"]PYY (25-36) RHYLQLITRQRY (SEQ ID NO: 95) [Gn", Leu 31 )PYY (25-36) RHYLQLLTRQRY (SEQ ID NO: 96) [Gin 29 , Ser 3 2 ]PYY (25-36) RHYLQLVSRQRY (SEQ ID NO: 97) [Gln 29 , Leu 3 ]PYY (25-36) RHYLQLVTKQRY (SEQ ID NO: 98) 30 [Gln 29 , Asnz]PYY (25-36) RHYLQLVTRNRY (SEQ ID NO: 99) [GinO, Leu"]PYY (25-36) RHYLQLVTRQKY (SEQ ID NO: 100) [Gn", Thr"l]PYY (25-36) RHYLQLVTRQRT (SEQ ID NO: 101) [Gin 2 9 , Phe"]PYY (25-36) RHYLQLVTRQRF (SEQ ID NO: 102) [Ile", Ile']PYY (25-36) RHYLNIITRQRY (SEQ ID NO: 103) 35 [Ile", Leu"]PYY (25-36) RHYLNILTRQRY (SEQ ID NO: 104) [IUc', Ser 2 ]PYY (25-36) RHYLNIVSRQRY (SEQ ID NO: 105) [Ile", Lys"]PYY (25-36) RHYLNIVTKQRY (SEQ ID NO: 106) [Ile", Asn]PYY(25-36) RHYLNIVTRNRY (SEQ ID NO: 107) [Ile, Lys 3 )PYY (25-36) RHYLNIVTRQKY (SEQ ID NO: 108) -55 [Ile", Thr"]PYY (25-36) RHYLNIVTRQRT (SEQ ID NO: 109) [Ie, Phe"]PYY (25-36) RHYLNIVTRQRF (SEQ ID NO: 110) [Val", leu"]PYY (25-36) RHYLNV1TRQRY (SEQ ID NO: 111) [Valm, Leu]PYY (25-36) RHYLNVLTRQRY (SEQ ID NO: 112) 5 [Val", Ser"]PYY (25-36) RHYLNVVSRQRY (SEQ ID NO: 113) [Val", Lys?]PYY (25-36) RHYLNVVTKQRY (SEQ ID NO: 114) [Val", Asn"]PYY (25-36) RHYLNVVTRNRY (SEQ ID NO: 115) [Val", Lys"]PYY (25-36) RHYLNVVTRQKY (SEQ ID NO: 116) [Val", Thre]PYY (25-36) RHYLNVVTRQRT (SEQ ID NO: 117) 10 [Va", Phe]PYY (25-36) RHYLNVVTRQRF (SEQ ID NO: 118) [IleP, Se 32 ]PYY (25-36) RHYLNLTSRQRY (SEQ ID NO: 119) [Ile", Lys"]PYY (25-36) RHYLNLITKQRY (SEQ ID NO: 120) [Ile", Asn"]PYY (25-36) RHYLNLITRNRY (SEQ ID NO: 121) [le", Lys 3 s]PYY (25-36) RHYLNLITRQKY (SEQ ID NO: 122) 15 [Ie", Thr']PYY (25-36) RHYLNLITRQRT (SEQ ID NO: 123) [Leu 31 , Phe"]PYY (25-36) RHYLNLITRQRF (SEQ ID NO: 124) [Leu 31 , Ser 3 3]PYY (25-36) RHYLNLLSRQRY (SEQ ID NO: 125) [VaL", Lys"]PYY (25-36) RHYLNLLTKQRY (SEQ ID NO: 126) [Leu, Asn"]PYY (25-36) RHYLNLLTRNRY (SEQ ID NO: 127) 20 [Leu", Lys"]PYY (25-36) RHYLNLLTRQKY (SEQ ID NO: 128) [Leu", Th']PYY (25-36) RHYLNLLTRQRT (SEQ ID NO: 129) [Leu, Phe]PYY (25-36) RHYLNLLTRQRF (SEQ ID NO: 130) [Se9 2 , Lys"]PYY (25-36) RHYLNLVSKQRY (SEQ ID NO: 131) Se2, Asn2']PYY (25-36) RHYLNLVSRNRY (SEQ ID NO: 132) 25 [Se 32 , Lys"]PYY (25-36) RHYLNLVSRQKY (SEQ ID NO: 133) [Ser", The']PYY (25-36) RHYLNLVSRQRT (SEQ ID NO: 134) [Se 2 , Phe"]PYY (25-36) RHYLNLVSRQRY (SEQ ID NO: 135) [Lys 33 , Asn 3 ]PYY (25-36) RHYLNLVTKNRY (SEQ ID NO: 136) [Lys 3 ", Lys"]PYY (25-36) RHYLNLVTKQKY (SEQ ID NO: 137) 30 [Lys", Thr"]PYY (25-36) RHYLNLVITKQRT (SEQ ID NO: 138) [Lys 33 , Phe"]PYY (25-36) RHYLNLVTKQRF (SEQ ID NO: 139) [Asn, Lys]PYY (25-36) RHYLNLVTRNKY (SEQ ID NO: 140) [Asn?, Thr 3 6]PYY (25-36) RHYLNLVTRNRT (SEQ ID NO: 141) [Asn3, Phe"]PYY (25-36) RHYLNLVTRNRF (SEQ ID NO: 142) 35 [Lys 3 , Thr']PYY (25-36) RHYLNLVTRQKT (SEQ ID NO: 143) [Lys", Phe 3 "]PYY (25-36) RHYLNLVTRQKF (SEQ ID NO: 144) Point Mutations of PYY(24-36) -56 PEPTIDE SEQUENCE PYY(24-36) LRHYLNLVTRQRY (SEQ ID NO: 145) [Ile1 4 ]PYY(24-36) IRHYLNLVTRQRY (SEQ ID NO: 146) [Va1 4 ]PYY(24-36) VRHYLNLVTRQRY (SEQ ID NO: 147) 5 Also included as PYY(24-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of any of these three mutants with any of the above listed mutants for PYY(25-36), e.g., [Lys 2 s]PPY(24 36) (Amino acid sequence=LKHYLNLVTRQRY (SEQ ID NO: 191)) would result 10 from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 145. Point Mutations of PYY(23-36) PEPTIDE SEQUENCE PYY(23-36) SLRHYLNLVTRQRY (SEQ ID NO: 148) 15 [Tbr]PYY(23-36) TLRHYLNLVTRQRY (SEQ ID NO: 149) Also included as PYY(23-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of either of these two mutants with any of the above listed mutants for PYY(25-36), and/or any of the 20 above listed mutants for PYY(24-36), e.g., [Lys5]PPY(23-36) (Amino acid sequence=SLKHYLNLVTRQRY (SEQ ID NO: 192)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 148. Point Mutations of PYY(22-36) 25 PEPTIDE SEQUENCE PYY(22-36) ASLRHYLNLVTRQRY (SEQ ID NO: 150) [Ser?)PYY(22-36) SSLRHYLNLVTRQRY (SEQ ID NO: 151) Also included as PYY(22-36) mutations are polypeptide variations (amino 30 acid sequence variations) resulting from the combination of either of these two mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(23-36), e.g., [Lys"JPPY(22-36) (Amino acid sequence=ASLKHYLNLVTRQRY (SEQ ID NO: 193)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 150.

-57 Point Mutations of PYY(21-36) PEPTIDE SEQUENCE PYY(21-36) YASLRHYLNLVTRQRY (SEQ ID NO: 152) 5 [Thr 2 ]PYY(21-36) TASLRHYLNLVTRQRY (SEQ DD NO: 153) [Phe 2 ]PYY(21-36) FASLRHYLNLVTRQRY (SEQ ID NO: 154) Also included as PYY(21-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of any of these three 10 mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(22-36), e.g., [Lys 25 ]PPY(21-36) (Amino acid sequence=YASLKHYLNLVTRQRY (SEQ ID NO: 194)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 152. 15 Point Mutations of PYY(20-36) PEPTIDE SEQUENCE PYY(20-36) YYASLRHYLNLVTRQRY (SEQ ID NO: 155) [Thr 2 ]PYY(20-36) TYASLRHYLNLVTRQRY (SEQ ID NO: 156) [Phem]PYY(20-36) FYASLRHYLNLVTRQRY (SEQ ID NO: 157) 20 Also included as PYY(20-36) mutations are polypeptide variations (amino acid scquence variations) resulting from the combination of any of these three mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(21-36), e.g., [Lys1 5 ]PPY(20-36) (Amino acid 25 sequence=YYASLKHYLNLVTRQRY (SEQ ID NO: 195)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 155. Point Mutations of PYY(19-36) PEPTIDE SEQUENCE 30 PYY(19-36) RYYASLRHYLNLVTRQRY (SEQ ID NO: 158) [Lys']PYY(19-36) KYYASLRHYLNLVTRQRY (SEQ ID NO: 159) Also included as PYY(19-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of either of these two -58 mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(20-36), e.g., [Lys"]PPY(19-36) (Amino acid sequence=RYYASLKHYLNLVTRQRY (SEQ ID NO: 196)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 158. 5 Point Mutations of PYY(1 8-36) PEPTIDE SEQUENCE PYY(18-36) NRYYASLRHYLNLVTRQRY (SEQ ID NO: 160) [Gln']PYY(18-36) QRYYASLRHYLNLVTRQRY (SEQ ID NO: 161) 10 Also included as PYY(18-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of either of these two mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(19-36), e.g., [Lys" 5 ]PPY(1 8-36) (Amino acid 15 sequence=NRYYASLKHYLNLVTRQRY (SEQ ID NO: 197)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 160. Point Mutations of PYY(1 7-36) PEPTIDE SEQUENCE 20 PYY(17-36) LNRYYASLRHYLNLVTRQRY (SEQ ID NO: 162) [Ile"IPYY(17-36) INRYYASLRHYLNLVTRQRY (SEQ ID NO: 163) [Val"]PYY(17-36) VNRYYASLRHYLNLVTRQRY (SEQ ID NO: 164) Also included as PYY(17-36) mutations are polypeptide variations (amino 25 acid sequence variations) resulting from the combination of any of these three mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(18-36), e.g., [Lys"]PPY(17-36) (Amino acid sequence=LNRYYASLKHYLNLVTRQRY (SEQ ID NO: 198)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 162. 30 Point Mutations of PYY(16-36) PEPTIDE SEQUENCE PYY(16-36) ELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 165) [Asp' 6 JPYY(16-36) DLNRYYASLRHYLNLVTRQRY (SEQ ID NO: 166) -59 Also included as PYY(l 6-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of either of these two mutants with any of the above listed mutants for PYY(25-36), and/or any of the 5 above listed mutants for PYY(17-36), e.g., [Lys"]PPY(16-36) (Amino acid sequence=ELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 199)) would result from combining the mutations from SEQ IID NO: 36 with SEQ ID NO: 165. Point Mutations of PYY(l 5-36) 10 PEPTIDE SEQUENCE PYY(15-36) EELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 167) [Asp"]PYY(15-36) DELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 168) Also included as PYY(15-36) mutations are polypeptide variations (amino 15 acid sequence variations) resulting from the combination of either of these two mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(16-36), e.g., [Lys"]PPY(15-36) (Amino acid sequence=EELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 200)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 167. 20 Point Mutations of PYY(14-36) PEPTIDE SEQUENCE PYY(14-36) PEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 169) 25 Also included as PYY(14-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of this PYY(14-36) mutant with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(15-36), e.g., [Lys 2 ]PPY(23-36) (Amino acid sequence=PEELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 201) would result 30 from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 169. Point Mutations of PYY(13-36) PEPTIDE SEQUENCE -60 PYY(13-36) SPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 170) [Thr"]PYY(13-36) TPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 171) Also included as PYY(13-36) mutations are polypeptide variations (amino 5 acid sequence variations) resulting from the combination of either of these two mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(14-36), e.g., [Lys2]PPY(13-36) (Amino acid sequence=SEELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 202)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 170. 10 Point Mutations of PYY(12-36) PEPTIDE SEQUENCE PYY(12-36) ASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 172) [Ser' 2 ]PYY(I 2-36) SSPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 173) 15 Also included as PYY(12-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of either of these two mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(13-36), e.g., [Lys 25 ]PPY(12-36) (Amino acid 20 sequence=ASEELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 203)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 172. Point Mutations of PYY(1 1-36) PEPTIDE SEQUENCE 25 PYY(11-36) DASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 174) [Glu"]PYY(1 1-36) EASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 175) Also included as PYY(1 2-3 6) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of either of these two 30 mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(12-36), e.g., [Lys2]PPY(l 1-36) (Amino acid sequence=DASEELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 204)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 174.

-61 Point Mutations of PYY(10-36) PEPTIDE SEQUENCE PYY(1O-36) EDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 176) [Asp' 0 ]PYY(10-36) DDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 177) 5 Also included as PYY(10-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of either of these two mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(1 1-36), e.g., [Lys"]PPY(10-36) (Amino acid 10 sequence=EDASEELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 205)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 176. Point Mutations of PYY(9-36) PEPTIDE SEQUENCE 15 PYY(9-36) GEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 178) Also included as PYY(9-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of this PPY(9-36) mutant with any of the above listed mutants for PYY(25-36), and/or any of the above listed 20 mutants for PYY(10-36), e.g., [Lys25]PPY(9-36) (Amino acid sequence=GEDASPEELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 206)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 178. 25 Potin Mutations of PYY(8-36) PEPTIDE SEQUENCE PYY(8-36) PGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 179) Also included as PYY(8-36) mutations are polypeptide variations (amino 30 acid sequence variations) resulting from the combination of this PPY(8-36) mutant with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(9-36), e.g., [Lys"]PPY(8-36) (Amino acid sequence= SEQ ID NO: 207)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 179.

-62 Point Mutations of PYY(7-36) PEPTIDE SEQUENCE PYY(7-36) APGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 180) 5 [Ser']PYY(7-36) SPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 181) Also included as PYY(7-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of either of these two mutants with any of the above listed mutants for PYY(25-36), and/or any of the 10 above listed mutants for PYY(8-36), e.g., [Lys 25 ]PPY(7-36) (Amino acid sequence=APGEDASEELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 208)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 180. 15 Point Mutations of PYY(6-36) PEPTIDE SEQUENCE PYY(6-36) EAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 182) [Asp']PYY(6-36) DAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 183) 20 Also included as PYY(6-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of either of these two mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(7-36), e.g., [Lys 2 s]PPY(6-36) (Amino acid sequence=EAPGEDASEELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 209)) 25 would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 182. Point Mutations of PYY(5-36) PEPTIDE SEQUENCE 30 PYY(5-36) PEAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 184) Also included as PYY(5-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of this PPY(5-36) mutant -63 with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(6-36), e.g., [Lys"]PPY(5-36) (Amino acid sequence=PEAPGEDASPEELNRYYASLKHYLNLVTRQRY (SEQ ID NO; 210)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 5 184. Point Mutations of PYY(4-36) PEPTIDE SEQUENCE PYY(4-26) KPEAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 185) 10 (Arg'JPYY(4-36) RPEAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 186) [Gln]PYY(4-36) QPEAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 187) [Asn 4 JPYY(4-36) NPEAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 188) Also included as PYY(4-36) mutations are polypeptide variations (amino 15 acid sequence variations) resulting from the combination of any of these four mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(5-36), e.g., [Lys 2 ]PPY(4-36) (Amino acid sequence=KPEAPGEDASEELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 211)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 20 185. Point Mutations of PYY(3-36) PEPTIDE SEQUENCE PYY(3-36) IKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 1) 25 [Leu 3 ]PYY(3-36) LKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 189) [Val) PYY(3-36) VKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY (SEQ ID NO: 190) Also included as PYY(3-36) mutations are polypeptide variations (amino acid sequence variations) resulting from the combination of any of these three 30 mutants with any of the above listed mutants for PYY(25-36), and/or any of the above listed mutants for PYY(4-36), e.g., [Lys 25 j]PPY(3-36) (Amino acid sequence=IKPEAPGEDASEELNRYYASLKHYLNLVTRQRY (SEQ ID NO: 212)) would result from combining the mutations from SEQ ID NO: 36 with SEQ ID NO: 1.

-64 Also contemplated are PYY agonists (NPY analogs) having the formula: 5 X-Q-Ri9 -R 20

-R

2 1

-R

22 -R23 -Leu-R 25

-R

2 6 -R27 -R2 8

-R

29

-R

30

-R

31 -R32 -Arg-R 34 Arg-R 36 -Y wherein X is H or Ca Me or Na Me or desamino or an acyl group having 7 carbon atoms or less; Q is R 1 7 -Rig, Rig or desQ; R 1 7 is Met, Arg, Nle, Nva, Leu, Ala 10 or D-Ala; R, 8 is Ala, Ser, Ile, D-Ala, D-Ser or D-Ile; Rig is Arg, Lys or Gln; R 20 is Tyr or Phe; R 2 1 is Tyr, Glu, His or Ala; R 2 2 is Ser, Ala, Thr, Asn or Asp; R 23 is Ala, Asp, Glu, Gln, Asn or Ser; R 25 is Arg or GIn; R 2 6 is His, Arg or Gin; R 2 7 is Phe or Tyr; R2s is Be, Leu, Val or Arg; R 29 is Asn or Ile; R30 is Leu, Met, Thr or Val; R 31 is Ile, Val or Leu; R 32 is Thr or Phe; R 34 is Gin, Pro or His; R 36 is Phe or Tyr; and Y is 15 NH 2 or OH; provided that when Q is RI 8 , then at least one of R 27 and R 36 is Phe. Analogs of NPY have the following applications: potent postsynaptic treatment of hypertension and cardiogenic shock, the treatment of acute cardiovascular circulatory failure, and the elevation of intracellular calcium. See U.S. Patent No. 5,026,685. 20 Certain preferred NPY analogs have the formula: X-R, 8 -Arg-Tyr-Tyr-R 22 R23-Leu-Arg-His-Tyr-R2g-Asn-Leu-R3,-Thr-Arg-Gln-Arg-Tyr-NH2, wherein X is H or Ca Me or N' Me or desamino or an acyl group having 7 carbon atoms or less; Ris is Ala or Ser, R 2 2 is Ser or Ala; R 2 3 is Ala or Ser; R 27 is Phe or Tyr; R2 8 is Ile or Leu;

R

31 is le or Val; and R 36 is Phe or Tyr; provided that at least one of R27 and R 36 is 25 Phe. See U.S. Patent No. 5,026,685. Other contemplated NPY analogs have the formula: X-R1 7 -RIB -Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-R27-De-Asn-Leu-Ile-Thr-Arg-Gln 30 Arg-R 36

-NH

2 , wherein R 17 is Arg or Leu and RIB is Ser or Ala or le; and wherein X, R 27 and R 36 are as previously indicated.

-65 Still other preferred NPY analogs have the formula: X-Rig-Arg-Tyr-Tyr-Ala-Ser-Leu-R25-His-R7-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg 5 R 3 6-NH 2 , wherein X is desamino or Ca Me or N' Me and wherein RI 8 , R 2 5 , R2 and R 36 arc as previously indicated. 10 Examples of such NPY agonists include: pNPY (17-36) having the formula: H-Leu-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln Arg-Tyr-NH2 (SEQ ID NO: 217) 15 The peptide hNPY (17-36) having the formula: H-Met-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-le-Asn-Leu-Ile-Thr-Arg-Gln Arg-Tyr-NH 2 (SEQ ID NO: 218) 20 The peptide [Phe 27 ]-NPY (18-36) having the formula: H-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Phe-Ie-Asn-Leu-Ile-Thr-Arg-Gln-Arg Tyr-NH 2 (SEQ ID NO: 219) The peptide [Ac-D-Ala"]-NPY (17-36) having the formula: 25 Ac-D-Ala-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg Gln-Arg-Tyr-NH 2 (SEQ ID NO: 220) - The peptide NPY (19-36) having the formula: H-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg-Tyr 30 NH 2 (SEQ ID NO: 221) The peptide [Nle 7 ]-NPY (17-36) having the formula: -66 H-Nle-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln Arg-Tyr-NH 2 (SEQ ID NO: 222) The peptide [D-Ser' 8 ]-NPY (18-36) having the formula: 5 H-D-Ser-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg Tyr-NH 2 (SEQ ID NO: 223) The peptide [Ala", His 2 l]-NPY (17-36) having the formula: H-Ala-Ala-Arg-Tyr-His-Ser-Ala-Leu-Arg-His-Tyr-De-Asn-Leu-Ile-Thr-Arg-Gln 10 Arg-Tyr-NH 2 (SEQ ID NO: 224) The peptide [D-Ile' 8 ]-NPY (18-36) having the formula: D-Ie-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-IlmAsn-Leu-Ile-Thr-Arg-Gln-Arg Tyr-NH 2 (SEQ ID NO: 225) 15 The peptide [Ac-Arg' 7 ]-NPY (17-36) having the formula: Ac-Arg-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ie-Asn-Leu-Be-Thr-Arg-Gln Arg-Tyr-NH 2 (SEQ ID NO: 226) 20 The peptide [Gln']-NPY (19-36) having the formula: H-Gln-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-De-Asn-Leu-Be-Thr-Arg-Gln-Arg-Tyr NiH 2 (SEQ ID NO: 227) The peptide [Phe 20 ]-NpY (18-36) having the formula: 25 H-Ala-Arg-Phe-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gln-Arg Tyr-NH 2 (SEQ ID NO: 228) The peptide [Ca MeLeu"]-pNPY (17-36) having the formula: H-Ca MeLeu-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Rle-Asn-Leu-Ile-Thr-Arg 30 Gln-Arg-Tyr-NH 2 (SEQ ID NO: 229) The peptide [N' MeLeu 7 ]-pNPY (17-36) having the formula: -67 H-Na MeLeu-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ilc-Asn-Leu-Ile-Thr-Arg Gln- Arg-Tyr-NH 2 (SEQ ID NO: 230) The peptide [desamino Ala' 8 ]-NpY (18-36) having the formula: 5 desamino-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg Gln-Arg-Tyr- NH 2 (SEQ ID NO: 231) The peptide [For-Ala" 8 , Glu 2 ', Arg 2 ]-NPY (18-36) having the formula: For-Ala-Arg-Tyr-Tyr-Ser-Glu-Leu-Arg-Arg-Tyr-le-Asn-Leu-Ile-Thr-Arg-Gln-Arg 10 Tyr-NH2 (SEQ ID NO: 232) The peptide [Nva 7 , Ala 21 , Leu"]-NPY (17-36) having the formula: H-Nva-Ala-Arg-Tyr-Ala-Ser-Ala-Leu-Arg-His-Tyr-Leu-Asn-Leu-Ile-Thr-Arg-Gln Arg-Tyr-NH 2 (SEQ ID NO: 233) 15 The peptide [Th? 2 , GIn3]-NPY (18-36) having the formula: H-Ala-Arg-Tyr-Tyr-Thr-Gln-Leu-Arg-His-Tyr-Ie-Asn-Leu-Ile-Thr-Arg-Gln-Arg Tyr-NH 2 (SEQ ID) NO: 234) 20 The peptide [desamino Leu 1 7, Asn", Val 3 ]-NPY (17-36) having the formula: H-desamino Leu-Ala-Arg-Tyr-Tyr-Ser-Asn-Leu-Arg-His-Tyr-Ile-Asn-Val-Ie-T Arg-Gln-Arg-Tyr-NH2 (SEQ ID NO: 235) 25 The peptide [Asp", Ser?, Th 3 ]-NPY (18-36) having the formula: H-Ala-Arg-Tyr-Tyr-Asp-Ser-Leu-Arg-His-Tyr-Ile-Asn-Thr-Ile-Thr-Arg-Gln-Arg Tyr-NH 2 (SEQ ID NO: 236) The peptide [Gin 5 , Leu 31 , Pro1 4 ]-NPY (18-36) having the formula: 30 H-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Gln-His-Tyr-Ile-Asn-Leu-Leu-Thr-Arg-Pro-Arg Tyr-NH2 (SEQ ID NO: 237) -68 The peptide [Gln 2 Phen-NPY (17-36) having the formula: H-Leu-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-Gln-Tyr-Arg-Asn-Leu-Ile-Thr-Arg-Gln Arg-Phe-NH 2 (SEQ ID NO: 238) 5 The peptide [Phe 3 ]-pPYY (19-36) having the formula: H-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Phe

NH

2 (SEQ ID NO: 239) The peptide pPYY (18-36) having the formula: 10 H-Ser-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg Tyr-NH2 (SEQ ID NO: 240) The peptide [Ac-Ser", Phe"]-pPYY (18-36) having the formula: Ac-Ser-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Phe-Leu-Asn-Leu-Val-Thr-Arg-Gln 15 Arg-Tyr-NH 2 (SEQ ID NO: 241) The peptide [Ne 17 , Asn 2 2 , Phe"]-NPY (17-36) having the formula: H-Nle-Ala-Arg-Tyr-Tyr-Asn-Ala-Leu-Arg-His-Phe-le-Asn-Leu-lle-Thr-Arg-Gln Arg-Tyr-NH 2 (SEQ ID NO: 242) 20 The peptide [D-Ala' 8 , GluZ t , His 34 ]-NPY (18-36) having the formula: H-D-Ala-Arg-Tyr-Glu-Ser-Ala-Leu-Arg-His-Tyr-Be-Asn-Leu-Ile-Thr-Arg-His-Arg Tyr-NH 2 (SEQ ID NO: 243) 25 The peptide [Bz-Leu 17 , Pro34, Phe"]-pNPY (17-36) having the formula: Bz-Leu-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-lle-Thr-Arg-Pro Arg-Phe-NH 2 (SEQ ID NO: 244) The peptide [Lys", Phe", Val 2 ]-NpY (18-36) having the formula: 30 H-Ala-Lys-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Phe-Val-Asn-Leu-Ile-Thr-Arg-Gln-Arg Tyr-NH 2 (SEQ ID NO: 245) -69 The peptide [D-Ala 17 , Val2s, Phe 32 ]-NPY (17-36) having the formula: D-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Val-Asn-Leu-Ile-Phe-Arg-Gln-Arg Tyr-NH 2 (SEQ ID NO: 246) The peptide [CMeSer Met ,Phe ]-NPY (18-36) having the formula: H-C" MeSer-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Met-Ile-Thr-Arg-Gln-Arg Phe-NH 2 (SEQ ID NO: 247) The peptide [Arg 17 , Ile", Phe 27 ,1]-NPY (17-36) having the formula: 10 H-Arg-Ile-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Phe-De-Asn-Leu-Il-Thr-Arg-Gln Arg-Phe-NH2 (SEQ ID NO: 248) The peptide [Ser 8 , Phe 27 ]-pNPY (17-36) having the formula: H-Leu-Ser-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Phe-Ile-Asn-Leu-Ie-Thr-Arg-Gln 15 Arg-Tyr-NH 2 (SEQ ID NO: 249) The peptide [Na Melle", Gln 5 , Phe" 7 ]-NPY (18-36) having the formula: N' Mele-Arg-Tyr-Tyr-Ser-Ala-Leu-Gin-His-Phe-Ile-Asn-Leu-Ile-Thr-Arg-Gln Arg-Tyr-NH 2 (SEQ ID NO: 250) 20 The peptide [D-Ser's, Phe"]-NPY (18-36) having the formula: H-D-Ser-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Lcu-Ile-Thr-Arg-Gln-Arg Phe-NH 2 (SEQ ID NO: 251) 25 The peptide [Asp 23 , Arg6]hNPY (17-36) having the formula: H-Met-Ala-Arg-Tyr-Tyr-Ser-Asp-Leu-Arg-Arg-Tyr-le-Asn-Leu-Ile-Thr-Arg-Gln Arg-Tyr-NH 2 (SEQ ID NO: 252) The peptide [Glu" 3 , Ile"]-NPY (18-36) having the formula: 30 H-Ala-Arg-Tyr-Tyr-Ser-Glu-Leu-Arg-His-Tyr-Ile-Ile-Leu-Ile-Thr-Arg-Gln-Arg Tyr-NH 2 (SEQ ID NO: 253) -70 The peptide [D-Ala 17 ]-NPY(17-36)-OH having the formula: D-Ala-Ala-Arg-Tyr-Tyr-Ser-Ala-Leu-Arg-His-Tyr-Ile-Asn-Leu-Ile-Thr-Arg-Gn Arg-Tyr-OH (SEQ ID NO: 254). 5 Other peptide YY agonists have the formula: RI R3 R2XAIAIII_26_A27 28 29 30 31 32

R

2

-X-A

2

-A

23

-A

24

-A

25 -A2 -A -A -An-A-A -A -Y-R 4 10 wherein: X is a chain of 0-5 amino acids, inclusive, the N-terminal one of which is bonded to R, and R 2 Y is a chain of 0-4 amino acids, inclusive, the C-terminal one of which is 15 bonded to R 3 and R 4 Ri is H, C1 -C 2 alkyl (e.g., methyl), C 6

-C

18 aryl (e.g., phenyl, napthaleneacetyl), C 1 -- C12 acyl (e.g., formyl, acetyl, and myristoyl), C7 -C 18 aralkyl (e.g., benzyl), or C7 -Cia alkaryl (e.g., p-methylphenyl);

R

2 is H, C 1

-C

12 alkyl (e.g., methyl), C6 -C 1 8 aryl (e.g., phenyl, 20 naphthaleneacetyl), C, -C 12 acyl (e.g., formyl, acetyl, and myristoyl), C7 -CIs aralkyl (e.g., benzyl), or C7 -C 18 alkaryl (e.g., p-methylphenyl); A 2 is an aromatic amino acid, Ala, Aib, Anb, N-Me-Ala, or is deleted;

A

23 is Ser, Thr, Ala, N-Me-Ser, N-Me-Thr, N-Me-Ala, or is deleted; AN is Leu, lie, Vat, Trp, Gly, Aib, Anb, N-Me-Leu, or is deleted; 25 A" is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-E-NH-R (where R is H, a branched or straight chain C, -Cio alkyl group, or an aryl group), Ore, or is deleted; A26 is His, Thr, 3-Me-His, 1-Me-His, #-pyrozolylalanine, N-Me-His, Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-E-NH-R (where R is H, a branched or straight chain C1 -Cio alkyl group, or an aryl group), Orn, or is deleted; 30 A 2 7 is an aromatic amino acid other than Tyr;

A

2 8 is Lou, Ile, Vat, Trp, Aib, Aib, Anb, or N-Me-Leu; A9 is Asn, Ala, Gln, Gly, Trp, or N-Me-Asn;

A

30 is Leu, Ile, Val, Trp, Aib, Anb, or N-Me-Leu;

A

3 ' is Vat, Ile, Trp, Aib, Anb, or N-Me-Val; -71

A

32 is Thr, Ser, N-Me-Set, or N-Me-Thr;

R

3 is H, CI -C1 2 alkyl (e.g., methyl), C 6 -CIS aryl (e.g., phenyl, naphthaleneacetyl), C, -C2 acyl (e.g., formyl, acetyl, and myristoyl), C7 -Cis aralkyl (e.g., benzyl), or C 7 -CIS alkaryl (e.g., p-methylphenyl); 5 R 4 is H, C, -C12 alkyl (e.g., methyl), C6 -CIS aryl (e.g., phenyl, naphthaleneacetyl), C, -C, 2 acyl (e.g., formyl, acetyl, and myristoyl), C7 -CIS aralkyl (e.g., benzyl), or C 7 -Cis alkaryl (e.g., p-methylphenyl), or a pharmaceutically acceptable salt thereof. See U.S. Patent No. 5,574,010. 10 Particularly preferred agonists of this formula to be used in the method of the disclosure include: N-a-Ala-Ser-Leu-Arg-His-Trp-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH 2 (SEQ ID NO: 255). 15 Other peptide YY agonists have the formula: Rt R3

R

2

-A

25

-A

26

-A

27 -A 2-A29-A3 -A3I-A -Y-R 4 20 wherein: the N-terminal amino acid bonds to R, and R 2 ; Y is a chain of 0-4 amino acids, inclusive the C-terminal one of which bonds to R3 and R4; R, is H, C, -C12 alkyl, C6 -CiS aryl, C1 -C 1 2 acyl, C7 -CiS aralkyl, or C7 -Cis 25 alkaryl;

R

2 is H, Cl -C12 alkyl, C6 -CS aryl, CI -C2 acyl, C7 -CS aralkyl, or C7 -CS alkaryl; A2 is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-E-NH-R (where R is H, a branched or straight chain C, -CIO alkyl group, or an aryl group), Orn, or is deleted; 30 A2 is Ala, His, Thr, 3-Me-His, 1-Me-His, P-pyrozolylalanine, N-Me-His, Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-e-NH-R (where R is H, a branched or straight chain Ci -Cio alkyl group, or an aryl group), Orn or is deleted; A2 is an aromatic amino acid; -72

A

8 is Leu, Ile, Val, Trp, Aib, Anb, or N-Me-Leu;

A

29 is Asn, Ala, Gln, Gly, Trp, or N-Me-Asn;

A

30 is Leu, ile, Val, Trp, Aib, Anb, or N-Me-Leu;

A

3 1 is Val, Ie, Trp, Aib, Anb, or N-Me-Val; 5 A32 is Thr, Set, N-Me-Set, or N-Me-Thr or D-Trp;

R

3 is H, CI-C 2 alkyl, C 6 -Cis aryl, C, -C12 acyl, C 7

-CI

8 aralkyl, or C 7 -CIS alkaryl; and

R

4 is H, CI -C1 2 alkyl, C 6

-CI

8 aryl, CI -C 2 acyl, C 7 -CIS aralkyl, or C 7 -CIS alkaryl, or a pharmaceutically acceptable salt thereof. Note that, unless indicated 10 otherwise, for all peptide YY agonists described herein, each amino acid residue, e.g., Leu and A', represents the structure of NH--C(R)H--CO--, in which R is the side chain. Lines between amino acid residues represent peptide bonds which join the amino acids. Also, where the amino acid residue is optically active, it is the L form configuration that is intended unless D-form is expressly designated. 15 Other PYY agonists have the formula: RI R2-- X-A22-A-A4-Aa'-AN-A27--A28--A29-A"

R

3

-AM--A-Y-R

4 wherein: X is a chain of 0-5 amino acids, inclusive, the N-terminal one of which is 20 bonded to R, and R 2 ; Y is a chain of 0-4 amino acids, inclusive, the C-terminal one of which is bonded to R 3 and R 4 ; RI is H, CI-C 2 alkyl (e.g. methyl), Cr-CIs aryl (e.g., phenyl, naphthaleneacetyl),

CI-C

2 acyl (e.g., fbrmyl, acetyl, and myristoyl), C 7 -Cjs aralkyl 25 (e.g., benzyl), or C 7 -CIs alkaryl (e.g., p-methylphenyl);

R

2 is H, CI-C 12 alkyl (e.g., methyl), C 6 -CIS aryl (e.g., phenyl, naphthaleneacetyl), Ci-C 2 acyl (e.g., formyl, acetyl, and myristoyl), C 7

-C

18 atalkyl (e.g., benzyl), or C 7 -C1S alkaryl (e.g., p-methylphenyl);

A

22 is an aromatic amino acid, Ala, Aib, Anb, N-Me-Ala, or is deleted; -73

A

3 is Ser, Thr, Ala, Aib, N-Me-Ser, N-Me-Thr, N Me-Ala, or is deleted;

A

24 is leu, Ile, Val, Trp, Gly, Nle, Nva, Aib, Anb, N-Me-Leu, or is deleted;

A

5 is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lye-e-NH-R (where R is H, a branched or straight chain C 1 -Cl 0 alkyl group, or an aryl group), Orn, or is deleted; 5 A6 is Ala, His, Thr, 3-Me-His, 1-Me-His, p-pyrozolylalanine, N-Me-His, Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-e-NH-R (where R is H, a branched or straight chain Ci-CIO alkyl groups or an aryl group), Orn, or is deleted;

A

27 is an aromatic amino acid other than Tyr;

A

2 is Leu, Ile, Val, Trp, Nle, Nva, Aib, Anb, or N-Me-Leu; 10 A 29 is Asn, Ala, Gin, Gly, Trp, or N-Me-Asn;

A

30 is Leu, Ile, Val, Trp, Nle, Nva, Aib, Anb, or N-Me-Leu; A" is Val, Leu, Ile, Trp, Nle, Nva, Aib, Anb, or N-Me-Val;

A

32 is Thr, Ser, N-Me-Ser, N-Me-Thr, or D-Trp;

R

3 is H, Ci-C 2 alkyl (e.g., methyl), C 6

-C,

8 aryl (e.g., phenyl, 15 naphthaleneacetyl),

CI-C

12 acyl (e.g., formyl, acetyl, and myristoyl), C 7 -Cig aralkyt (e.g., benzyl), or C 7 -Cig alkaryl (e.g., p-methylphenyl); and

R

4 is H, Ci-C 2 alkyl (e.g., methyl), C6-CIs aryl (e.g., phenyl, naphthaleneacetyl),

CI-C

2 acyl (e.g., formyl, acetyl, and myristoyl), C 7 -Cls aralkyl (e.g., benzyl), or C 7 -Cis alkaryl (e.g., p-methylphenyl), or a pharmaceutically 20 acceptable salt thereof. In preferred embodiments, A 27 is Phe, Nal, Bip, Pcp, Tic, Trp, Bth, Thi, or Dip. In preferred embodiments X is A17-A'-A -A 2-A2 wherein

A

7 is Cys, Leu, Ile, Val, Nle, Nva, Aib, Anb, or N-Me-Leu; 25 A' 8 is Cys, Ser, Thr, N-Me-Ser, or N-Me-Thr;

A'

9 is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-E-NH-R (where R is H, a branched or straight chain C-CIO alkyl group, or C 6 -CIs aryl group), Cys, or Orn;

A

20 is an aromatic amino acid, or Cys; and

A

21 is an aromatic amino acid, Cys, or a pharmaceutically acceptable salt 30 thereof. In yet other preferred embodiments, Y is A 3

-A

34 -A -A 3 wherein A" is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-e-NH-R (where R is H, a branched or straight chain CI-CI 0 alkyl group, or an aryl group), Cys, or Orn; -74

A

3 4 is Cys, Gln, Asn, Ala, Gly, N-Me-Cln, Aib, or Anb; A35 is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-E-NH-R (where R is H, a branched or straight chain Cl-Clo alkyl group, or C 6 -Cis aryl group), Cys, or Orn; and 5 A 36 is an aromatic amino acid, Cys or a pharmaceutically acceptable salt thereof. See U.S. Patent No. 5,604,203. Particular embodiments include compounds has the formula: N-a-Ac-Ala Ser-Leu-Arg-His-Phe-Leu-Asn-Leu-Val-Thr-Arg-Gin-Arg-Tyr-NH2 (SEQ. ID. NO: 325), H-Ala-Ser-Leu-Arg-His-Phe-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH2 10 (SEQ. ID. NO: 326), N-a-Ac-Ala-Ser-Leu-Arg-Thr-Arg-Gin-Arg-Tyr-NH 2 (SEQ. ID. NO: 327), N-a-Ac-Ala-Ser-Leu-Arg-His-Thi-Leu-Asn-Leu-Val-Thr-Arg-Gin Arg-Tyr-NH 2 (SEQ. ID. NO: 328), N-a-Ac-Tyr-Ser-Leu-Arg-His-Phe-Leu-Asn Leu-Val-Thr-Arg-Gin-Arg-Tyr-NH 2 (SEQ. ID. NO: 329) or a pharmaceutically acceptable salt thereof. 15 Other PYY agonists have the formula: (Formula 11) R1 ?-W -31 2y R4

R

2 -A -A --A-A -A 29 -As 0 -A -A 32 --- R 4 wherein the N-terminal amino acid is bounded to R, and R 2 ; Y is a chain of 20 0-4 amino acids, inclusive the C-terminal one of which is bonded to R 3 and R 4 ; R, is H, CI-C 12 alkyl (e.g., methyl), C 6 -Ci8 aryl (e.g., phenyl, naphthaleneacetyl), CI-C 12 acyl (e.g., formyl, acetyl, and myristoyl), C 7

-C,

8 aralkyl (e.g., benzyl), or C 7 -Ci 8 alkaryl (e.g., p-methylphenyl);

R

2 is H, C1-C 1 2 alkyl (e.g., methyl), C 6

-CI

8 aryl (e.g., phenyl, 25 naphthaleneacetyl), CI-C 1 2 acyl (e.g., formyl, acetyl, and myristoyl), C7-Cis aralkyl (e.g., benzyl), or C 7

-C

18 alkaryl (e.g., p-methylphenyl);

A

3 is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-s-NH-R (where R is H, a branched or straight chain CI-Cio alkyl group, or an aryl group), Orn, or is deleted; -75

A

26 is Ala, His, Thr, 3-Me-His, 1-Me-His, P-pyrozolylalanine, N-Me-His, Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-c-NH-R (where R is H, a branched or straight chain CI-Clo alkyl groups or an aryl group), Orn, or is deleted; A27 is an aromatic amino acid; 5 A 28 is Leu, Ile, Val, Trp, Nle, Nva, Aib, Anb, or N-Me-Leu; A2 is Asn, Ala, Gin, Gly, Trp, or N-Mc-Asn;

A

30 is Leu, Ile, Val, Trp, Ne, Nva, Aib, Anb, or N-Me-Leu;

A

3 1 is Val, Ile, Trp, Nlc, Nva, Aib, Anb, or N-Me-Val;

A

32 is Thr, Ser, N-Me-Ser, N-Me-Thr, or D-Trp; 10 R 3 is H, C-C 2 alkyl (e.g., methyl), C 6 -Cjs aryl (e.g., phenyl, naphthaleneacetyl), C-C 12 acyl (e.g., formyl, acetyl, and myristoyl), C 7

-C

1 i aralkyl (e.g., benzyl), or C 7

-C

18 alkaryl (e.g., p-methylphenyl); and R4 is H, C-C1 2 alkyl (e.g., methyl), C 6

-C

18 aryl (e.g., phenyl, naphthaleneacetyl), C-C 12 acyl (e.g., formyl, acetyl, and myristoyl), C 7 -Cig aralkyl 15 (e.g., benzyl), or C 7 -Cs alkaryl (e.g., p-methylphenyl), or a pharmaceutically acceptable salt thereof See U.S. Patent No. 5,604,203. In particular embodiments, A 27 is Phe, Nal, Bip, Pcp, Tic, Trp, Bth, Thi, or Dip. In particular embodiments X is A 33

-A

4

-A

35

-A

3 - wherein 20 AD is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-e-NH-R (where R is H, a branched or straight chain C-Cio alkyl group, or C-C1 8 aryl group), Cys, or Orn; A34 is Gin, Asn, Ala, Gly, N-Me-Gin, Aib, Cys, or Anb;

A

35 is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-E-NH-R (where R is H, a branched or straight chain C-Cio alkyl group, or C 6 -Clg aryl group), Cys, or Orn; 25 and

A

6 is an aromatic amino acid, Cys, or a pharmaceutically acceptable salt thereof. Preferably, the compound has the formula: N-cr-Ac-Arg-His-Phe-Leu-Asn Leu-Val-Thr-Arg-Gln-Arg-Tyr-NH 2 (SEQ. ID. NO: 324). 30 Examplary PYY agonists include: YPAKEAPGEDASPEELSTYYASLR [im-DNP-His S ID NO: 256) -76

YLNLVTRZRY-NH

2 PYY(22-36)

ASLRHYLNLVTRQRY-NH

2 (SEQ ID NO: 257) [Ala")PYY ASLRHYLNLV[Ala]RQRY-NH 2 (SEQ ID NO: 258) [Ala 2 32 ]PYY A[Ala]LRHYLNLV[Ala]RQRY-NN 2 (SEQ ID NO: 259) {Glu 2 )PYY(22-36) ASLRHY[Glu]NLVTRQRY-NH 2 (SEQ ID NO: 260) N-a-Ac-PYY(22-36) N-a-Ac-ASLRHYLNLVTRORY.NH 2 (SEQ ID NO: 261) N-cx-Ac[p,CL.Phe 6 ]PYY N-a-Ac-ASLR[p.CL.Phe 26

]YLNLVTRQRY-NH

2 (SEQ ID NO: 262) N-a-Ac[Gu 28 ]PYY N-ot-Ac-ASLRHY[Glu]NLVTRQRY-NH 2 (SEQ ID NO: 263) N-a-Ac[Phe 7 ]PYY N-a-Ac-ASLRH[Phe]ENLVTRQR[N-Me-Tyr]-NH 2 (SEQ ID NO: 264) N-a-Ac]8N-Me-Tyrl 6 ]PYY N-a-Ac-ASLRHYENLVTROR[N-Me-Tyr]-NH 2 (SEQ ID NO: 265) N-aX-myristoyl-PYY(2214 36) N-ca-myristoyl-ASLRHYLNLVTRQRY-NH 2 (SEQ ID NO: 266) N-a-naphthateneacetyl-PYY(22-36) N-ct- naphthateneacetyl-ASLRHYLNLVTRQRY-NH 2 (SEQ ID NO: 267) N-ca-Ac[Phe 27 ]PYY N-a-Ac-ASLRH[Phe]ENLVTRR[N-Me-Tyr]-NH 2 (SEQ ID NO: 268) N-a-Ac-PYY (22-36) N-L-Ac-ASLRHYLNLVTRQRY-NH 2 (SEQ ID NO: 269) N-a-Ac-{Bth 27 1PYY (22-36) N-a-Ac-ASLRH[Bth]LNLVTRQRY-NH 2 (SEQ ID NO: 270) N-a-Ac-[Bip 2 l]PYY (22-36) (SEQ ID NO: 271) N-ca-Ac-ASLRH[Bth]LNLVTRQRY-NH 2 (SEQ ID NO: 272) N-a-Ac-[Nal 27 JPYY (22-36) N-a-Ac-ASLRH[Bth]LNLVTRQRY-NH 2 (SEQ ID NO: 273) N-a-Ac-[TrpF]PYY (22-36) (SEQ ID NO: 274) N-a-Ac-ASLRH[Trp]LNLVTRQRY-NH 2 (SEQ ID NO: 275) N-a-Ac-[Thi 27 ]PYy (22-36) N-a-Ac-ASLRN[Thi]LNLVTRQRY-NH 2 (SEQ ID NO: 276) N-cc-Ac-{Tic 27 ]PYY (22-36) N-aX-Ac-ASLRH[Tic]LNLVTRQRY-NH 2 (SEQ ID NO: 277) N-a-Ac-[Phe 27 ]PYY (25-36) N-a-Ac-H[Phe]LNLVTRQRY-NH 2 (SEQ ID NO: 279) N-a-Ac-[Phe 27 ,Thi 27 ]PYY (22-36) N-c-Ac-ASLRH[Phe]LNLVTRQR[Thi]-NH 2 (SEQ ID NO: 280) N-ac-Ac-[Phz',Phe]PYY (22-36) N-cx-Ac-ASLRH[Thz][Phe]LNLVTRQRY-NH 2 (SEQ ID NO: 281) -77 N-a-Ac-[Phe]PYY (22-36) N-a-Ac-ASLRH[Thz][Phe]LNLVTRQRY-NH 2 (SEQ ID NO: 282) N-a-Ac-[Phe 27 ]PYY (22-36) N-a-Ac-[Phe]SLRN[Phe]LNLVTRQRY-NH 2 (SEQ ID NO: 289) N-x-Ac-[Tyr",Phe 27 ]PYY (22-36) N-a-Ac-[Tyr]SLRH[Phe]LNLVTRQRY-NH 2 (SEQ ID NO: 290) N-a-Ac-[Trp 2 8 ]PYY (22-36) N-a-Ac-ASLRHY[Trp]NLVTRQRY-NH 2 (SEQ ID NO: 291) N-a-Ac-[Trp 2 8 ]PYY (22-36) N-a-Ac-ASLRHYLN[Trp]VTRQRY-NH 2 (SEQ ID NO: 292) N-a-Ac-[Aa 2 6 ,Phe"]PYY (22-36) N-a-Ac-ASLR[Ala][Phe]LNLVTRQRY-NH 2 (SEQ ID NO: 293) N-a-Ac-[Bth 27 ]PYY (22-36) N-a-Ac-ASLR[Btb]LNLVTRQRY-NH 2 (SEQ ID NO: 294) N-a-Ac-[Phe 27 ]PYY (22-36) N-a-Ac-ASLRH[Phe]LNLVTRQRY-NH 2 (SEQ ID NO: 295) N-a-Ac-[Phe 7 ']PYY (22-36) N-a-Ac-ASLRH[Phe]LNLVTRQR[Phe]-NHz (SEQ ID NO: 296) N-a-Ac-[Phe 2 1, D-Trp 32 ]PYY (22-36) N-a-Ac-ASLRH[Phe]LNLV[D-Trp]RQRY-NH2 (SEQ ID NO: 297) Other PYY agonists include neurophilic Y Y2 receptor specific peptides having the formula: Xl(-X2-X3-X4-X5-X6-X7-X8-X9-XIO-XI 1-X12-X13-XI4),-XI5 5 wherein X1 is NH, CH 3 CO or one or two naturally occurring amino acids. X2 is Leu, Ile or Val. X3 is Arg, Lys or His. X4 is His, Lys or Arg. 10 X5 is Tyr or Phe. X6 is Leu, Ile or Val. X7 is Asn or Gln. X8 is Leu, Ile or Val. X9 is Leu, le or Val. 15 X10 is Thr or Ser. X1I is Arg, His or Lys. X12 is Gin or Asn.

-78 X13 is Arg, His or Lys. XI 4 is Tyr or Phe. XI5 is COOH, NH 2 or one or two naturally occurring amino acids with the terminal amino acid being in the normal or carboxamide form; and 5 n is I to 5. See U.S. Patent No. 5,696,093. Examplary agonists include:

CH

3 CO-L-R-H-Y-L-N-L-L-T-R-Q-R-Y-NH2 (SEQ ID NO: 298)

CH

3

CO-L-R-H-Y-I-N-L-I-T-R-Q-R-Y-NH

2 (SEQ ID NO: 299) 10 NH2-L-R-H-Y-L-N-L-L-T-R-Q-R-Y-NH 2 (SEQ ID NO: 300)

NH

2

-L-R-H-Y-I-N-L-I-T-R-Q-R-Y-NH

2 (SEQ ID NO: 301) Other PYY agonists have the formula: N-a-R1 -[Ne 24

,

30 , TrpV, NVa 3 I, ;p 3 5

/

3 6 IPYY(22-36)-NH2, 15 N-a-R' -[Nle 24

'

2 ', Trp 27

'

3 0 , Nva 31 , 3 5' ]PYY(22-36)-NH 2 , N-a-R' -[Nle 2 4 ,3'2o, Phe 2 7 , Nva" t , g35/ 3 s ]PYY(22-36)-NH 2 , N-a-R -[Nle 24 ,, Phe', Trp 30 , Nva 3t , l35/36 ]PYY(22-36)-NH 2 , N-a-R -[Trp 30 , 03"36 ]PYY(25-36)-NH2, N-a-R' -[Trp 3 0 ]PYY(25-36)-NH 2 , 20 N-a-R' -[Nle 24

,

2 , Trp 30 , NVa 1 , '3"}15 JPYY(22-36)-NH 2 and N-a-Ri -[Nem, Trp 3 0 , NVa 3 l, 0356 ]PYY(22-36)-NH 2 or a pharmaceutically acceptable salt thereof, wherein R' is H, (CI -C, 2 )alkyl or (CI -C,2)acyl; and iP is a pseudopeptide bond selected from the group consisting of --CH 2

-NH

25 -, -- H 2 -S--, --CH 2

-CH

2 -- , --CH2 --0-- and -- CH 2 --CO--. See U.S. Patent No. 6,046,162. Particular compounds of the immediately foregoing group of compounds are where R 1 is acetyl and is --CH 2 --NH-. 30 A particular group of compounds is selected from a group consisting of N-a-Ac-[Ne 24 ,.3, Trp 27 , Nva 31 , V35/"3 ]PYY(22-36)-NH 2 , (SEQ ID NO: 302) -79 N-a-Ac-[Nle 24

,

2 ., Trp" 0 , Nva, ,33 ]PYY(22-36)-NH 2 , (SEQ ID NO: 303) N-a-Ac-[Nle 2 4

,

30 , Phe 27 , NVa #3/36 ]PYY(22-36)-NH 2 , (SEQ ID NO: 304) 5 N-a-Ac-[Ne 24 ,2 8 , Phe 27 , Trp 3 0 , Nva 3 , 336 ]PYY(22-36)-NH 2 , (SEQ ID NO: 305) N-a-Ac-[Trp 30 , 0"1 6 ]PYY(25-36)-NH 2 , (SEQ ID NO: 306) N-a-Ac-[Trp 30 ]PYY(25-36)-NH 2 (SEQ ID NO: 307) and N-a-Ac-[Nje 2 , Trp 30 , Nva", #3536 ]PYY(22-36)-NH 2 , (SEQ ID NO: 308) or 10 a pharmaceutically acceptable salt thereof. Another particular compound has the formula N-a-Ac-[Nle 24

,

28 , Trp 3 0 , Nva.sup.

31 , v3536 ]PYY(22-36)-NH 2 (SEQ. ID. NO: 309) or a pharmaceutically acceptable salt thereof. Another PYY agonist has the formula (A), 15 R R 3

R

2

-RI-A

2 -Aa-A 2 4

-A

5

-A

26 -Fla 27 -A 2

-A

2 9

-A

30

-A

3 1

-A-

32

R

20

-R

4 20 having one or two pseudopeptide bonds where each pseudopeptide bond is independently selected from the group consisting of --CH 2 --NH-, -CH 2 --S--, -

CH

2

--CH

2 --, --CH 2 --0-- and -CH 2 -CO-; wherein: R1 0 is a chain of 0-5 amino acids, inclusive, where the N-terminal amino acid 25 is bonded to R1 and R 2 by the side chain of the N-terminal amino acid or by the nitrogen of the amino group of the N-terminal amino acid;

R

20 is a chain of 0-4 amino acids, inclusive, where the C-terminal amino acid is bonded to R 3 and R 4 by the side chain of the C-terminal amino acid or by the carbon of the carboxyl group of the C-terminal amino acid; 30 R', R 2 , R 3 and R 4 are each independently selected from the group consisting of H, (CI -C 12 )alkyl, (C 6 -Cis)aryl, (CI -C 12 )acyl, phenyl(Ci -C1 2 )alkyl and ((CI C12)alkyl).s -phenyl; A2 is an aromatic amino acid, Ala, Aib, Anb, N-Me-Ala or is deleted;

A

23 is Ser, Thr, Ala, N-Me-Ser, N-Me-Thr, N-Me-Ala or is deleted; -80

A

24 is Leu, lie, Nle, Val, Trp, Gly, Aib, Anb, N-Me-Leu or is deleted; A25 is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-p.epsilon.-NH-Z, Orn or is deleted;

A

26 is His, Thr, 3-Me-His, 1-Me-His, 0-pyrazolylalanine, N-Me-His, Arg, 5 Lys, homo-Arg, diethyl-homo-Arg, Lys-e-NH-Z, Orn or is deleted;

A

2 8 is Leu, Ile, Nle, Val, Trp, Aib, Anb or N-Me-Leu;

A

29 is An, Ala, Gin, Gly, Trp or N-Me-Asn; A30 is Leu, Ile, Ne, Fla, Val, Trp, Aib, Anb or N-Me-Leu; A 3 is Val, Nva, Ile, Trp, Aib, Anb or N-Me-Val; and 10 A2 is Thr, Ser, N-Me-Ser or N-Me-Thr; where Z for each occurrence is independently selected from the group consisting of H, (C 1 -Cto)alkyl and (C 6 -Cig)aryl; or a pharmaceutically acceptable salt thereof. See U.S. Patent No. 6,046,167. A particular group of compounds of the immediately foregoing group of 15 compounds is where RIO is A 7

-A'

8 19 -'A 20 -A; where A1 7 is Cys, Leu, Ile, Val, Nle, Nva, Aib, Anb or N-Me-Leu;

A'

8 is Cys, Ser, Thr, N-Me-Scr or N-Me-Thr;

A'

9 is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-E-NH-R.sup.5, Cys or Orn; 20 A 20 is an aromatic amino acid or Cys;

A

2 ' is an aromatic amino acid or Cys;

R

20 is A 3 -AM -A 35

-A

36 , A" is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-E-NH-Rs, Cys or Orn;

A

3 4 is Cys, Gin, Asn, Ala, Gly, N-Me-Gin, Aib or Anb; 25 A 35 is Arg, Lys, homo-Arg, diethyl-homo-Arg, Lys-E-NH-R 5 , Cys or Orn; and

A

36 is an aromatic amino acid or Cys; where R 5 for each occurrence is independently selected from the group consisting of H, (C, -Cio)alkyl and (C 6 -C1S) aryl. 30 -81 A particular group of compounds of the foregoing group of compounds are the compounds of the formula N-a-Ac-[Fla 2 7 )]PYY(25-36)-NH 2 and N-a-Ac-[Fla 27 ]PYY(22-36)-NH 2 or a pharmaceutically acceptable salt thereof. 5 Another group of PYY agonist has the formula: (1) (R' R)-A' -A 2

-A

3 -A' -A 5 -A' -AB -A 9 -A 0 -Ra 0 , 10 (R'R 2

)-A-A-A

3

-A'-A'-A'-A

7

-A-A-A"-R

0 \ / / \ 15 (R'R 2

)-A'-A

2

-A

3

-A

4 -A6-A4-A-A-A'-R" (113) (R' R 2 )-[A' -A' -A 7 -A' -A' A" ]. R , 20 or a pharmaceutically acceptable salt thereof wherein - represents an optional bond between the amino acids shown connected where each bond is independently selected from the group consisting of --S--Sonly when the amino acids connected are Cys-Cys, -CO-NH-, -CH 2 -NH- and 25 -HN-CH-Co

(CH

2 )4 30 -HN-CH-CO provided that when the optional bond is -HN-CH-Co 35

(CH

2

)

4

-HN-CH-CO

40 it replaces the two amino acids that the optional bond is attached to; q is 1-4; m is 1 to 4; -82 RO is OH or -O-R', provided that when A' to A 7 are deleted then R 30 is also NH-R1, where R 30 is attached to the carbon atom of the carboxyl of the C-terminal amino acid; R' and R 2 for each occurrence are each independently selected from the 5 group consisting of H, (CI -C1 2 )alkyl, (C 6 -Cl8)aryl, (CI -C1 2 )acyl, phenyl(CI C] 2 )alkyl and ((CI -C 2 )alkyl)1.s -phenyl where R' and R 2 are attached to the nitrogen of the amine of the N-terminal amino acid; A' is deleted or D- or L- of the following amino acids: Trp, Tyr, Fla, Bth, Nal, Tic, Tic-OH, Dip, Bip or optionally substituted Phe where the Phe is optionally 10 substituted with one to five substituents selected from the group consisting of (CI C 4 )alkyl, halo, (CI -C 4 )alkoxy, amino and nitro;

A

2 is deleted or D- or L- of the following amino acids: Ile, Val, Leu, Nle, Anb, Aib, Pro, Gln or Asn;

A

3 is deleted or D- or L- of the following amino acids: Asn, Gln, Glu, Asp, 15 Orn, Lys, Dpr or Cys;

A

4 is deleted or D- or L- of the following amino acids: Ile, Val, Leu, Ne, Anb, Aib or Pro; As is deleted or D- or L- of the following amino acids: Ile, Val, Leu, Ne, Anb, Aib, Pro, Glu, Asp, Orn, Lys, Dpr or Cys; 20 A 6 is deleted or D- or L- of the following amino acids: Thr, Ser, Trp, Tyr, Fla, Bth, Nal, Tic, Tic-OH, Dip, Bip or optionally substituted Phe where the Phe is optionally substituted with one to five substituents selected from the group consisting of (CI -C4)alkyl, halo, (CI -C 4 )alkoxy, amino and nitro; A2 is deleted or D- or L- of the following amino acids: Arg, Lys, homo-Arg, 25 dialkyl-homo-Arg, Lys-E-NH-R or Om; AB is deleted or D- or L- of the following amino acids: Nva, Val, Ile, Leu, Nle, Anb, Aib, Pro, Gin, Asn, Glu, Asp, Orn, Lys, Dpr or Cys; A? is deleted or D- or L- of the following amino acids: Arg, Lys, homo-Arg, dialkyl-homo-Arg, Lys-e-NH-R 7 or Orn; and 30 A' 0 is deleted or D- or L- of the following amino acids: Tyr, Trp, Fla, Bth, Nal, Tic, Tic-OH, Dip, Bip, tyramine or optionally substituted Phe where the Phe is optionally substituted with one to five substituents selected from the group -83 consisting of (CI -C 4 )alkyl, halo, (CI -C 4 )alkoxy, amino and nitro, or the corresponding decarboxylated optionally substituted Phe; where R 7 for each occurrence is independently selected from the group consisting of H.sub., (CI -CIo)alkyl and (C 6

-CI

8 ) aryl, provided that not all of At to 5 Aio are deleted at the same time. See U.S. Patent No. 6,046,167. A particular group of compounds of the immediately foregoing group of compounds is (SEQ ID NO: 310) H--Ie--Asn-Pro--Be--Tyr-Arg--Leu-Arg-Tyr--OMe 10 (SEQ ID NO: 311) H-Ile--Asn--Pro--Cys-Tyr--Arg--Leu-Arg--Tyr-Ome 15 H-Ile--Asn-Pro--Cys--Tyr--Arg--Leu--Arg--Tyr--Ome, (SEQ ID NO: 312) H--Cys-Tyr--Arg--Leu--Arg--Tyr--Ome 20 1 H--Cys--Tyr-Arg--Leu-Arg-Tyr-OMe, 25 (SEQ ID NO: 313) H--Ile--Asn--Pro--NH-CH--CO--Tyr--Arg--Leu--Arg--Tyr--OMe

(CH

2

)

4 30 H-le--Asn--Pro-NH--CH-CO--Tyr-Arg--Leu--Arg--Tyr--OMe, (SEQ ID NO: 314) H-[Tyr-Arg-Leu-Arg-Tyr] 2 -Ome or a pharmaceutically acceptable salt thereof. 35 PYY and PYY agonists may be produced by recombinant DNA technology by chemical synthesis, from natural sources, or by any combination thereof. PYY and PYY agonists may be modified by well known processes such as amidation, -84 glycosylation, acylation (e.g. acetylation), sulfation, phosphylation, cyclization, lipidization and pegylation. Methods for lipidization with fatty acid derivatives of sulfhydryl-containing compounds are disclosed in U.S. Patent No. 5,936,092; U.S. Patent No. 6,093,692; and U.S. Patent No. 6,225,445. Fatty acid derivatives of 5 sulfhydryl-containing PYY and PYY agonists comprising fatty acid-conjugated products with a disulfide linkage are employed for delivery of the PYY and PYY agonists to neuronal cells and tissues. This modification markedly increases the absorption of the compounds relative to the rate of absorption of the unconjugated compounds, as well as prolonging blood and tissue retention of the compounds. 10 Moreover, the disulfide linkage in the conjugate is quite labile in the cells and thus facilitates intracellular release of the intact compounds from the fatty acid moieties. Fatty acids, as constituents of phospholipids, make up the bulk of cell membranes. Due to their lipidic nature, fatty acids can easily partition into and interact with the cell membrane in a non-toxic way. Therefore, fatty acids represent 15 potentially a useful carrier ligand for the delivery of proteins and peptides. Strategies that may use fatty acids in the delivery of proteins and peptides include the covalent modification of proteins and peptides and the use of fatty acid emulsions. To prepare such conjugates, a sulfhydryl-containing PYY and PYY agonist 20 is attached to a fatty acid derivative via a reversible, biodegradable disulfide bond. Such a conjugate is expected to bind to the apical side of a cell membrane, reach the basolateral membrane of the GI-epithelium as a result of membrane transport and turnover, and become released into interstitial fluid as the result of disulfide bond reduction. 25 Such lipidized PYY and PYY agonist compounds have the general formula SOR 3 P--S-S--CH2 -NH(C=)R 2 RI in which P is a residue derived from a PYY or PYY agonist; R' is hydrogen, lower alkyl or aryl; R 2 is a lipid-containing moiety and R 3 is --OH, a lipid-containing 30 moiety or an amino acid chain comprising one or 2 amino acids and terminating in - -85

CO

2 H or -COR 2 . See U.S. Patent No. 5,936,092. These conjugates are particularly useful for increasing the absorption and prolonging blood and tissue retention of PYY and PYY agonists. Typical alkyl groups include C 1 .6 alkyl groups including methyl, ethyl, 5 propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, 2-pentyl, 3-pentyl, neopentyl, hexyl, 2-hexyl, 3-hexyl, 2-methyl-l-pentyl, 3-methyl-I-pentyl, 4-methyl 1-pentyl, and the like. Preferred aryl groups are C 6

.

14 aryl groups and typically include phenyl, naphthyl, fluorenyl, phenanthryl, and anthracyl groups. 10 The term "lipid-containing moiety" refers to either a lipid group per se or a hydrocarbon-based group (in particular, one or more amino acids) comprising a lipid group. By the term "lipid group" is meant a hydrophobic substituent consisting of 4 to 26 carbon atoms, preferably 5 to 19 carbon atoms. Suitable lipid groups include, but are not limited to, the following: palmityl (Ci 5

H

31 ,), oleyl (C 15

H

29 ), stearyl 15 (C 17

H

3 ), cholate; and deoxycholate. PCT Application No. WO 00/34236 describes drug-carrier conjugates and synthetic strategies for their production, as well as synthetic methods, intermediates, and final products useful for the uptake and release of biologically-active amino group containing compounds. Such lipidized PYY and PYY agonist compounds 20 have general Formula I 0 2 R4 R5 R3 -c(Y) x o in which R 2 is selected from the group consisting of hydrogen, halo, alkyl, or aryl, wherein the alkyl or aryl groups are optionally substituted with one or more alkoxy, alkoxyalkyl, alkanoyl, nitro, cycloalkyl, alkenyl, alkynyl, alkanoyloxy, alkyl or 25 halogen atoms; R is a lipophilic group; one of R 4 and R 5 is a PYY or a PYY agonist and the other of R 4 and R 5 is ORe where R 6 is hydrogen, an alkali thetal or a negative charge; -86 X is oxygen or sulfur; Y is a bridging natural or unnatural amino acid; n is zero or 1; and m is an integer from zero to 10. Typical alkyl groups include C 1

.

6 alkyl groups including methyl, ethyl, 5 propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, 2-pentyl, 3-pentyl, neopentyl, hexyl, 2-hexyl, 3-hexyl, 2-methyl-l-pentyl, 3-methyl-1-pentyl, 4-methyl 1-pentyl, and the like. Typical alkoxy groups include oxygen substituted by any of the alkyl groups mentioned above. 10 Typical alkoxyalkyl groups include any of the above alkyl groups substituted by an alkoxy group, such as methoxymethyl, ethoxymethyl, propoxymethyl, butoxymethyl, pentoxymethyl, hexoxymethyl, methoxyethyl, methoxypropyl, methoxybutyl, methoxypentyl, methoxyhexyl, and the like. Preferred aryl groups are C644 aryl groups and typically include phenyl, 15 naphthyl, fluorenyl, phenanthryl, and anthracyl groups. Typical alkoxy substituted aryl groups include the above aryl groups substituted by one or more of the above alkoxy groups, e.g., 3-methoxyphenyl, 2 ethoxyphenyl, and the like. Typical alkyl substituted aryl groups include any of the above aryl groups 20 substituted by any of the CI.6 alkyl groups, including the group Ph(CH 2 )n, where n is 1-6, for example, tolyl, o-, m-, and p-xylyl, ethylphenyl, I -propylphenyl, 2 propylphenyl, 1-butylphenyl, 2-butylphenyl, t-butylphenyl, i-pentylphenyl, 2 pentylphenyl, 3-pentylphenyl. Typical alkenyl groups include C2-6 alkenyl groups, e.g. ethenyl, 2-propenyl, 25 isopropenyl, 2-butenyl, 3-butenyl, 4-pentenyl, 3-pentenyl, 2-pentenyl, 5-hexenyl, 4 hexenyl, 3-hexenyl, and 2-hexenyl groups. Typical alkynyl groups include C 2

.

6 alkynyl groups e.g. enthynyl, 2 propenyl, 2-butynyl, 3-butynyl, 4-pentynyl, 3-pentynyl, 2-pentynyl, 5-hexynyl, 4hexynyl, 3-hexynyl, and 2-hexynyl groups. 30 Typical alkenyl or alkynyl substituted aryl groups include any of the above C.14 aryl groups substituted by any of the above C2.6 alkenyl or C2.6 alkynyl groups, e.g., ethenylphenyl, 1-propenylphenyl, 2-propenylphenyl, lbutenylphenyl, 2- -87 butenylphenyl, 1-pentenylphenyl, 2-pentenylphenyl, 3-pentenylphenyl, 1 hexenylphenyl, 2-hexenylphenyl, 3-hexenylphenyl, ethynylphenyl, 1 propynylphenyl, 2-propynylphenyl, 1-butynylphenyl, 2-butynylphenyl, 1 pentynylphenyl, 2-pentynylphenyl, 3-pentynylphenyl, 1-hexynylphenyl, 2 5 hexynylphenyl, 3-hexynylphenyl groups. Typical halo groups include fluorine, chlorine, bromine, and iodine. Typical halo substituted alkyl groups include Cr-6 alkyl groups substituted by one or more fluorine, chlorine, bromine, or iodine atoms, e.g., fluoromethyl, difluoromethyl, trifluoromethyl, pentafluoroethyl, 1,1-difluoroethyl, and 10 trichloromethyl groups. Typical alkanoyl groups include C 1

.

5 C(=O)- alkanoyl groups, e.g., acetyl, propionyl, butanoyl, pentanoyl, and hexanoyl groups, or by an arylalkanoyl group, e.g., a C 1 .sC(=O) - alkanoyl group substituted by any of the above aryl groups. Typical cycloalkyl groups include C 3

.

8 cycloalkyl groups including 15 cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl groups. The term "lipophilic group" as used herein refers to either a naturally occurring lipid per se, a hydrophobic branched or unbranched hydrocarbon comprising about 4 to about 26 carbon atoms, preferably about 5 to about 19 carbon 20 atoms, a fatty acid or ester thereof, or a surfactant. Suitable lipophilic groups include, but are not limited to, long chain alkanoyl groups including: palmityl

(C

15

H

31 ), oleyl (Ci 5 Hz 9 ), stearyl (CiyH 35 ), lauryl (CuIH 23 ), cholyl, and myristyl

(C

13

H

27 ) The term "natural or unnatural amino acid" as used herein refers to any of the 25 21 naturally occurring amino acids as well as D-form amino acids, blocked L-and D form amino acids such as those blocked by amidation or acylation, substituted amino acids (e.g., those substituted with a sterically hindered alkyl group or a cycloalkyl group such as cyclopropyl or cyclobutyl) in which the substitution introduces a conformational restraint in the amino acid. The preferred naturally occurring amino 30 acids for use in the present disclosure as amino acids or components of a peptide or protein are alanine, arginine, asparagine, aspartic acid, citrulline, cysteine, cystine, y-glutamic acid, glutamine, glycine, histidine, isoleucine, norleucine, leucine, lysine, -88 methionine, ormithine, phenylalanine, proline, hydroxyproline, serine, threonine, tryptophan, tyrosine, valine,'y -carboxyglutamate, or 0-phosphoserine. The preferred non-naturally occurring amino acids for use in the present disclosure as amino acids or components of peptides or proteins are any of the 0-amino acids, e.g., 5 a'-alanine, y-amino butyric acid, -y-amino butyric acid, L(aminophenyl)butyric acid, a-amino isobutyric acid, e-amino caproic acid, 7-amino heptanoic acid, amino benzoic acid, aminophenyl acetic acid, aminophenyl butyric acid, cysteine (ACM), methionine sulfone, phenylglycine, norvaline, ornithine, 6-ornithine, pnitro phenylalanine, 1,2,3,4-terahydroisoquinoline-3-carboxylic acid and thioproline. 10 The present disclosure is also directed to methods of preparing lipidized conjugates of PYY and PYY agonists, pharmaceutical compositions comprising lipidized conjugates of PYY and PYY agonists, and methods of increasing the delivery of amino group-containing PYY and PYY agonists into a cell. Also provided by the disclosure are chemically modified derivatives of PYY 15 and PYY agonists which may provide additional advantages such as increased solubility, stability and circulating time of the polypeptide, or decreased immunogenicity (see U.S. Patent No. 4,179,337). Such modified derivatives include PYY and PYY agonists modified by pegylation. The terms "pegylated" and "pegylation" refer to the process of reacting a poly(alkylene glycol), preferably an 20 activated poly(alkylene glycol), with a facilitator such as an amino acid, e.g. lysine, to form a covalent bond. Although "pegylation" is often carried out using poly(ethylene glycol) or derivatives thereof, such as methoxy poly(ethylene glycol), the term is not intended to be so limited here, but is intended to include any other useful poly(alkylene glycol), such as, for example poly(propylene glycol). 25 The chemical moieties for derivitization may also be selected from water soluble polymers such as polyethylene glycol, ethylene glycol/propylene glycol copolymers, carboxymethylcellulose, dextran, polyvinyl alcohol and the like. The polypoptides may be modified at random positions within the molecule, or at predetermined positions within the molecule and may include one, two, three or 30 more attached chemical moieties. The polymer may be of any molecular weight, and may be branched or unbranched. For polyethylene glycol, the preferred molecular weight is between -89 about I kDa and about 100 kDa (the term "about" indicating that in preparations of polyethylene glycol, some molecules will weigh more, some less, than the stated molecular weight) for ease in handling and manufacturing. Other sizes may be used, depending on the desired therapeutic profile (e.g., the duration of sustained release 5 desired, the effects, if any on biological activity, the ease in handling, the degree or lack of antigenicity and other known effects of the polyethylene glycol to a therapeutic protein or analog). For example, the polyethylene glycol may have an average molecular weight of about 200, 500, 1000, 1500, 2000, 2500, 3000, 3500, 4000, 4500, 5000, 5500, 6000, 6500, 7000, 7500, 8000, 8500, 9000, 9500, 10,000, 10 10,500, 11,000, 11,500, 12,000, 12,500, 13,000, 13,500, 14,000, 14,500, 15,000, 15,500, 16,000, 16,500, 17,000, 17,500, 18,000, 18,500, 19,000, 19,500, 20,000, 25,000, 30,000, 35,000, 40,000, 50,000, 55,000, 60,000, 65,000, 70,000, 75,000, 80,000, 85,000, 90,000, 95,000, or 100,000 kDa. As noted above, the polyethylene glycol may have a branched structure. 15 Branched polyethylene glycols are described, for example, in U.S. Patent No. 5,643,575; Morpurgo et al., AppL. Biochem. Biotechnol. 56:59-72, 1996; Vorobjev et al., Nucleosides Nucleotides 18:2745-2750, 1999; and Caliceti et al., Bioconjug. Chem. 10:638-646, 1999. The polyethylene glycol molecules (or other chemical moieties) should be 20 attached to the polypeptides or proteins with consideration of effects on functional or antigenic domains of the polypeptides or proteins. There are a number of attachment methods available to those skilled in the art, e.g., EP 0 401 384 (coupling PEG to G-CSF), see also Malik et al., Exp. Hematol. 20:1028-1035, 1992 (reporting pegylation of GM-CSF using tresyl chloride). For example, polyethylene glycol 25 may be covalently bound through amino acid residues via a reactive group, such as, a free amino or carboxyl group. Reactive groups are those to which an activated polyethylene glycol molecule may be bound. The amino acid residues having a free amino group may include lysine residues and the N-terminal amino acid residues; those having a free carboxyl group may include aspartic acid residues glutamic acid 30 residues and the C-terminal amino acid residue. Sulfhydryl groups may also be used as a reactive group for attaching the polyethylene glycol molecules. Preferred for -90 therapeutic purposes is attachment at an amino group, such as attachment at the N-terminus or lysine group. As suggested above, polyethylene glycol may be attached to proteins and polypeptides via linkage to any of a number of amino acid residues. For example, 5 polyethylene glycol can be linked to proteins and polypeptides via covalent bonds to lysine, histidine, aspartic acid, glutamic acid, or cysteine residues. One or more reaction chemistries may be employed to attach polyethylene glycol to specific amino acid residues (e.g., lysine, histidine, aspartic acid, glutamic acid, or cysteine) of the polypeptide or protein or to more than one type of amino acid residue (e.g., 10 lysine, histidine, aspartic acid, glutamic acid, cysteine and combinations thereof) of the protein or polypeptide. One may specifically desire proteins and polypeptides chemically modified at the N-terminus. Using polyethylene glycol as an illustration, one may select from a variety of polyethylene glycol molecules (by molecular weight, branching, etc.), 15 the proportion of polyethylene glycol molecules to protein (or peptide) molecules in the reaction mix, the type of pegylation reaction to be performed, and the method of obtaining the selected N-terminally pegylated protein. The method of obtaining the N-terminally pegylated preparation (i.e., separating this moiety from other monopegylated moieties if necessary) may be by purification of the N-terminally 20 pegylated material from a population of pegylated protein molecules. Selective proteins chemically modified at the N-terminus modification may be accomplished by reductive alkylation which exploits differential reactivity of different types of primary amino groups (lysine versus the N-terminal) available for derivatization in a particular protein. Under the appropriate reaction conditions, substantially selective 25 derivatization of the protein at the N-terminus with a carbonyl group containing polymer is achieved. As indicated above, pegylation of the proteins and polypeptides may be accomplished by any number of means. For example, polyethylene glycol may be attached to the protein or polypeptide either directly or by an intervening linker. 30 Linkerless systems for attaching polyethylene glycol to proteins and polypeptides are described in Delgado et al., Crit. Rev. Thera. Drug Carrier Sys. 9:249-304, -91 1992; Francis et al., Intern. J. ofHematol. 68:1-18, 1998; U.S. Patent No. 4,002,531; U.S. Patent No. 5,349,052; WO 95/06058; and WO 98/32466. One system for attaching polyethylene glycol directly to amino acid residues of proteins and polypeptides without an intervening linker employs tresylated 5 MPEG, which is produced by the modification of monmethoxy polyethylene glycol (MPEG) using tresylchloride (CISO 2

CH

2

CF

3 ). Upon reaction of the protein or polypeptide with tresylated MPEG, polyethylene glycol is directly attached to amine groups of the protein or polypeptide. Thus, the disclosure includes protein polyethylene glycol conjugates produced by reacting proteins and polypeptides with 10 a polyethylene glycol molecule having a 2,2,2-trifluoreothane sulphonyl group. Polyethylene glycol can also be attached to proteins and polypeptides using a number of different intervening linkers. For example, U.S. Patent No. 5,612,460 discloses urethane linkers for connecting polyethylene glycol to proteins. Protein polyethylene glycol conjugates wherein the polyethylene glycol is attached to the 15 protein or polypeptide by a linker can also be produced by reaction of proteins or polypeptides with compounds such as MPEG-succinimidylsuccinate, MPEG activated with 1,1'-carbonyldiimidazole, MPEG-2,4,5-trichloropenylcarbonate, MPEG- p -nitrophenolcarbonate, and various MPEG-succinate derivatives. A number of additional polyethylene glycol derivatives and reaction chemistries for 20 attaching polyethylene glycol to proteins and polypeptides are described in WO 98/32466. The number of polyethylene glycol moieties attached to each protein or polypeptide (i.e., the degree of substitution) may also vary. For example, the pegylated proteins and polypeptides may be linked, on average, to 1, 2, 3, 4, 5, 6, 7, 25 8, 9, 10, 12, 15, 17, 20, or more polyethylene glycol molecules. Similarly, the average degree of substitution within ranges such as 1-3, 2-4, 3-5, 4-6, 5-7, 6-8, 7-9, 8-10, 9-11, 10-12, 11-13, 12-14, 13-15, 14-16, 15-17, 16-18, 17-19, or 18-20 polyethylene glycol moieties per protein or polypeptide molecule. Methods for determining the degree of substitution are discussed, for example, in Delgado et al., 30 Crit. Rev. Thera. Drug Carrier Sys. 9:249-304, 1992. The proteins and polypeptides containing substantially non-antigenic polymers, preferably poly(alkylene glycols) may be prepared, for example, as -92 described in U.S. Patent No. 5,428,128; U.S. Patent No. 6,127,355; and U.S. Patent No. 5,880,131. To effect covalent attachment of poly(ethylene glycol) (PEG) to a protein or polypeptide, the hydroxyl end groups of the PEG must first be converted into 5 reactive functional groups. This process is frequently referred to as "activation" and the product is called "activated PEG." Methoxy poly(ethylene glycol) (mPEG), distally capped with a reactive functional group is often used. One such activated PEG is succinimidyl succinate derivative of PEG (SS-PEG). See also Abuchowski et al., Cancer Biochem. Biophys. 7:175-186, 1984; and U.S. Patent No. 5,122,614 10 which discloses poly(ethylene glycol)-N-succinimide carbonate and its preparation. Alternative substantially non-antigenic polymers that may be employed in the practice of the present disclosure include materials such as dextran, polyvinyl pyrrolidones, polysaccharides, starches, polyvinyl alcohols, polyacrylamides, or other similar non-immunogenic polymers. Those of ordinary skill in the art will 15 realize that the foregoing are merely illustrative and not intended to restrict the type of polymeric substances suitable for use herein. In one aspect of the disclosure, the polymer is introduced into the peptide or protein molecule after being functionalized or activated for reaction and attachment to one or more amino acids. By activation, it is understood by those of ordinary skill 20 in the art that the polymer is functionalized to include a desired reactive group. See, for example, U.S. Patent No. 4,179,337 and U.S. Patent No. 5,122,614. In this embodiment, the hydroxyl end groups of poly(alkylene glycols) are converted and activated into reactive functional groups. In another aspect of the disclosure, the polymer is conjugated to a facilitator 25 moiety prior to being introduced into the polypeptide or protein molecule. The facilitator moiety is preferably an amino acid such as lysine, however, non-amino acid moieties are also contemplated. Within the aspect, there are included multifunctionalized organic moieties such as alkyls or substituted alkyls. Such moieties can be prepared to have a nucleophilic functional group such as an amine 30 and an electrophilic group such as an acid as well as a suitably functionalized region for conjugating with the desired polymer or polymers. The facilitator moieties allow easier inclusion of a polymer into the peptide -93 or protein molecule during synthesis. For example, poly(alkylene glycols) coupled to facilitator amino acids or amino acid residues in polypeptides or proteins by means of suitable coupling agents are illustrative. A useful review of a number of coupling agents known in the art appears in Dreborg et al., Critical Reviews in 5 Therapeutic Drug Carrier Systems 6(4):315-165, 1990, see especially, pp. 317-320. Pegylated PYY peptides and agonists can also be of the general formula Y R1 D--Y'--C-(Chr---X--R3 R2 wherein: D is a residue of a PYY peptide or agonist; 10 X is an electron withdrawing group; Y and Y' are independently 0 or S; (n) is zero (0) or a positive integer, preferably from I to about 12; RI and R 2 are independently selected from the group consisting of H, C 1

.

6 alkyls, aryls, substituted aryls, aralkyls, heteroalkyls, substituted heteroalkyls, and 15 substituted C 1

.

6 alkyls;

R

3 is a substantially non-antigenic polymer, CI-2 straight or branched alkyl or substituted alkyl, Css cycloalkyl or substituted cycloalkyl, carboxyalkyl, carboalkoxy alkyl, dialkylaminoalkyl, phenylalkyl, phenylaryl or R4 N-Rs; and 20 R 4 and R 5 are independently selected from the group consisting of H, C 1

.

6 alkyls, aryls, substituted aryls, aralkyls, heteroalkyls, substituted heteroalkyls and substituted C1.6 alkyls or jointly form a cyclic C 5

-C

7 ring. See U.S. Patent No. 6,127,355. Typical alkyl groups include CI.6 alkyl groups including methyl, ethyl, 25 propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, 2-pentyl, 3-pentyl, neopentyl, hexyl, 2-hexyl, 3-hexyl, 2-methyl-l-pentyl, 3-methyl-1-pentyl, 4-methyl 1-pentyl, and the like.

-94 Preferred aryl groups are C6-1 aryl groups and typically include pheny, naphthyl, fluorenyl, phenanthryl, and anthracyl groups. Typical alkyl substituted aryl groups include any of the above aryl groups substituted by any of the C1.6 alkyl groups, including the group Ph(CH 2 )n, where n is 5 1-6, for example, tolyl, o-, m-, and p-xylyl, ethylphenyl, 1 -propylphenyl, 2 propylphenyl, I -butylphenyl, 2-butylphenyl, t-butylphenyl, I -pentylphenyl, 2 pentylphenyl, 3-pentylphenyl. Typical cycloalkyl groups include C 3

.

8 cycloalkyl groups including cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl 10 groups. Typical electron withdrawing groups include 0, NR 1 , S, SO and SO 2 , wherein R, is defined above. GLP-1 and Agonists Thereof 15 GLP- 1 is produced from preproglucogon, which is a 160 amino acid polypeptide, in the central nervous system (CNS) and the intestine. It is released into the circulation in response to nutrient intake. Physiological actions of GLP-1 in man include stimulation of insulin release, suppression of gastric acid secretion and 20 slowing of gastric emptying. GLP-1 (1-37) (SEQ ID NO: 336) is the initial product of the processing of preproglucagon. GLP-1 (1-37) is amidated by post-translational processing to yield GLP-1 (1-36) NH (SEQ ID NO 337), or is enzymatically processed to give GLP-1 (7-37) (SEQ ID NO: 338). GLP-l (7-37) can be amidated to give GLP-1 (7-36) 25 amide (SEQ ID NO: 339). The sequences of human GLP-1 are given below: GLP-1 (1-37): His Asp Glu Phe Glu Arg His Ala Glu Gly Thr Phe The Ser Asp Val Ser Ser Tyr Leu Gu Gly Gly Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly (SEQ ID NO: 336). 30 -95 GLP-1 (1-36) amide: His Asp Glu Phe Glu Arg His Ala Glu Gly Thr Phe The Ser Asp Val Ser Ser Tyr Leu Glu Gly Gly Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg NH 2 (SEQ ID NO: 337), 5 GLP-1 (7-37): His Ala Glu Gly Thr Phe The Ser Asp Val Ser Ser Tyr Leu Glu Gly Gly Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly (SEQ ID NO: 338). GLP-1 (7-36) amide: His Ala Glu Gly Thr Phe The Ser Asp Val Ser Ser Tyr Leu Glu Gly Gly Ala Ala Lys Glu Phe fle Ala Trp Leu Val Lys Gly Arg NH 2 10 (SEQ ID NO: 339). A GLP-1 agonist is a peptide, small molecule, or chemical compound that preferentially binds to the GLP- 1 receptor and stimulates the same biological activity as does GLP-1. In one embodiment, an agonist for the GLP-1 receptor 15 binds to the receptor with an equal or greater affinity than GLP-l. In another embodiment, an agonist selectively binds the GLP-1 receptor, as compared to binding to another receptor. Exendin-4, which is a 39-amino acid peptide isolated from the salivary glands of the Gila monster (Heloderma suspectum) (Eng J et al J Biol Chem 267:7402-7405, 1992) is an example of an agonist at the GLP-1 receptor. 20 Molecules derived from exendin-4 and that also have GLP-1 agonist activity are further examples of GLP-1 agonists. GLP-1 agonists include GLP-1 related peptides and peptides that result from natural or synthetic enzymatic or chemical processing of preproglucagon or of a GLP-1 peptide or a related peptide. Any compound that is described as being a GLP-1 agonist may be used in 25 the present invention, as may any compound that is tested for GLP-1 agonist activity, for example, as described above, and found to function as a GLP- I agonist. A recombinant GLP- I receptor suitable for use in screening is disclosed in WO93/19175. Many GLP-l agonists are known and are described in the art. Examples of published patent specifications that disclose GLP-l agonists are the 30 following: W02002/67918, W02002/66479, W02002/03978, W02001/89554, W02001/14386, W02001/66135, WO2001/35988, W02001/14368, W02001/04156, W02000/78333, W02000/59887, W02000/42026, EP 0955314, -96 and W099/43707. Examples of GLP-1 agonists are Arg34, Lys26(N-epsilon (gamma-Glu(N-alpha-hexadecanoyl)))-GLP-1 (7-37), IP7-GLP-1 (7-37)OH. GLP-1 or an agonist thereof may be administered according to the present invention peripherally at a dose of, for example, 0.1 moles or more per kg body 5 weight of the subject, for example, 0.2 nmoles or more, for example, 0.4 nmoles or more, for example, 0.6 nmoles or more, for example, 0.8 moles or more, for example, 1.0 mole or more, for example, 1.2 moles or more, for example, 1.4 nmoles or more, for example, 1.6 nmoles or more, for example, 1.8 moles or more, for example, 2.0 rnoles or more, for example, 2.2 moles or more, for example, 2.4 10 nmoles or more, for example, 2.6 nmoles or more, for example, 2.8 nimoles , for example, 3.0 moles or more, for example, up to 3.2 nmoles per kg body weight. The amount used may be up to 3.0 nmoles per kg body weight, for example, up to 2.8 moles, for example, up to 2.6 nmoles, for example, up to 2.4 moles, for example, up to 2.2 moles, for example, up to 2.0 nmoles, for example, up to 1.8 15 nmoles, for example, up to 1.4 nmoles, for example, up to 1.2 moles, for example, up to 1.0 moles, for example, up to 0.8 nmoles, for example, up to 0.6 nmoles, for example, up to 0.4 moles, for example, up to 0.2 nmoles per kg body weight. The dose is generally in the range of from 0.1 to 3.2 nmoles per kg body weight, for example, within any combination of upper and. lower ranges given above. 20 We have shown that intracerebroventricular (ICV) administration of GLP-1 to rats potently inhibits food intake. This effect is blocked by the specific GLP-l receptor antagonist, exendin 9-39. GLP-1 receptors are found in the brainstem, arcuate nucleus and PVN. Following ICV GLP-1 administration, c-fos is expressed in the arcuate nucleus and PVN. However, peripheral administration of GLP-1 in 25 man and rat also inhibits food intake and results in c-fos expression in the brainstem of rats. As is disclosed herein, the effect on I-hour food intake in non-fasted rats of IP administration of GLP-1 is not influenced by the presence of concomitant exendin 9-39, a GLP-1 receptor agonist, in the arcuate nucleus of the rat. This indicates that circulating GLP- I acts via the brainstem rather than the arcuate 30 nucleus. These and other findings suggest that the main site for appetite inhibition by peripheral GLP-1 is the dorsal vagal complex, acting in part directly through the -97 area postrema. However, the physiological importance of this in man is currently unclear. As disclosed herein, when administered to humans, PYY was found to reduce appetite. When infused into humans at physiological post-prandial levels, 5 PYY3.

36 significantly decreased appetite and reduced food intake by a third over 12 hours, and even by a third over 24 hours. Both the effect itself and the duration of the effect are surprising and unpredictable, as they occurred for many hours after the hormone had been cleared from the circulation. The effects, which are produced at physiological levels of the peptide, are strong indications that PYY acts in vivo to 10 regulate feeding behavior. As disclosed herein, peripheral administration of PYY 3

-

36 in the rat caused an increase of c-fos immunoreactivity in the arcuate nucleus of the hypothalamus and a decrease in hypothalamic neuropeptide Y (NPY) mRNA. Further, electro physiological studies demonstrated that PYY 3

.

36 inhibits synaptic activity of the 15 NPY nerve terminals and thus activates POMC neurons, which are known to receive inhibitory NPY synaptic inputs. Without being bound by theory, these results demonstrate that the gut hormone PYY 3.36 can act via the neuropeptide Y Y2 receptor. This hypothesis is supported by the observation that when PYY 3.36 was administered to neuropeptide 20 Y Y2 receptor null mice (Y2R gene knock out mice), no inhibition of feeding was observed. Administration of PYY 3-36 to wild type littermates of the Y2R null mice was fully effective in inhibiting feeding. Thus, a novel gut-brain pathway that inhibits feeding after meals is described. Without being bound by theory, the natural pathway involves release of 25 PYY from the gut, its conversion to PYY 3.36, which acts as an agonist on the neuropeptide Y Y2 receptor (NPY Y2 receptor) in the brain. The NPY Y2 receptor acts as a inhibitory pre-synaptic receptor reducing release of neuropeptide Y, which is a most potent stimulator of feeding, and also acting on the anorexigenic melanocortin systems, the result of the NPY Y2 receptor activity being to suppress 30 appetite and decrease food intake. The action of PYY 3.36 may occur in the arcuate nucleus of the hypothalamus, but other areas may be also be involved.

-98 The results obtained show that PYY 3

-

36 , a gut hormone that circulates in the blood, inhibits appetite at physiological concentrations, and that the inhibitory effect is observed even for some hours after the hormone has been cleared from the blood. This effect has been observed in all species tested, i.e. in mouse, rat and human. The 5 circulating gut hormone appears to act via hypothalamic circuits. The reduction of messenger RNA, necessary for the synthesis of brain appetite regulating hormones, in particular of hypothalamic NPY mRNA may be a possible mechanism for the long action of PYY 3.36. When PYY 3 .36 was co-administered parenterally, either IP or IV with GLP-1, 10 a clear synergistic reduction in food intake was produced. This contrasts with the results obtained with oxyntomodulin, where there was no synergistic effect, only a sum of the individual effects. As indicated above, PYY 3

.

36 is considered to act via the arcuate nucleus. Oxynotmodulin is also considered to act via that region of the brain. GLP-1, however, is believed to act via the brainstem, as explained above. 15 While not being limited to the following, it appears that, when agents that act via different areas of the brain and/or by different neurological routes are administered parenterally, they produce a synergistic affect, whereas when they act by the same area and/or route they do not. These results indicate the importance in vivo of the interaction of gut 20 hormones on the regulation of appetite and food intake. The present invention demonstrates that, surprisingly, it is possible to regulate appetite and food intake more effectively by informed choice of agents. The disclosure is illustrated by the following non-limiting Examples. 25 EXAMPLES Example 1 Material and Methods 30 Generation ofPOMC-EGFP mice: The EGFP cassette contains its own Kozak consensus translation initiation site along with SV40 polyadcnylation signals downstream of the EGFP coding sequences directing proper processing of the 3' end -99 of the EGFP mRNA. The EGFP cassette was introduced by standard techniques into the 5' untranslated region of exon 2 of a mouse Pomc genomic clone containing 13 kb of 5' and 2 kb of 3' flanking sequences (Young et al., JNeurosci 18, 6631-40, 1998). The transgene was microinjected into pronuclei of one-cell stage embryos of 5 C57BLJ6J mice (Jackson Laboratories) as described (Young et al., JNeurosci 18, 6631-40, 1998). One founder was generated and bred to wildtype C57BL/6J to produce N, hemizygous mice. In addition, N 2 and subsequent generations of mice homozygous for the transgene were also generated. The mice are fertile and have normal growth and development. 10 Immunofluorescence and GFP co-localization: Anesthetized mice were perfused transcardially with 4% paraformaldehyde and free-floating brain sections prepared with a vibratome. Sections were processed for immunofluorescence and colocalization of GFP fluorescence using standard techniques. Primary antisera and their final dilutions were rabbit anti-p-endorphin, 1:2500 v/v; rabbit anti-NPY, 15 1:25,000 v/v (Alanex Corp.); rabbit anti-ACTH, 1:2000 v/v; and mouse anti-TH, 1:1000 v/v (Incstar). After rinsing, sections were incubated with 10mg/ml biotinylated horse anti-mouse/rabbit IgG (Vector Laboratories) followed by Cy-3 conjugated streptavidin, 1:500 v/v (Jackson Immunoresearch Laboratories). Photomicrographs were taken on a Zeiss Axioscop using FITC and RITC filter sets 20 (Chroma Technology Corp.). Electrophysiology (Example 2): 200 prm thick coronal slices were cut from the ARC of four-week old male POMC-EGFP mice. Slices were maintained in (in mM) [NaCl, 126; KCl, 2.5; MgCl2, 1.2; CaCl 2 .2H 2 0, 2.4; NaH 2

PO

4

.H

2 0, 1.2; 25 NaHCO 3 , 21.4; Glucose, 11.1] (Krebs) at 35*C and saturated with 95% 02 5% C02 for 1 hour(hr) prior to recordings. Recordings were made in Krebs at 35* C. Slices were visualized on an Axioskop FS2 (Zeiss) through standard infra red optics and using epifluoresence through a FITC filter set (see Fig. 1 c). Whole cell recordings were made from fluorescent neurons using an Axopatch 1D amplifier (Axon 30 Instruments) and Clampex 7 (Axon Instruments). Resting membrane potentials were determined using an event detection protocol on a PowerLab system (AD Instruments, Mountain View, CA) to average expanded traces of the membrane -100 potential. Drugs were applied to the bath over the times indicated. The resting membrane potential was stable for up to an hour in cells treated with Krebs alone. I-V relationships for the Met-Enk currents were established using a step protocol; ( 60 mV holding potential, sequentially pulsed (40 ms) from -120 to -50 mV, cells 5 were returned to -60 mV for 2 s between voltage steps). The protocol was repeated after Met Enk addition. The net current was the difference between the two I-V relationships. This protocol was repeated in Krebs with 6.5 mM K. I-V relationships to identify the postsynaptic leptin current were performed similarly with slow voltage ramps (5 mV/ s from -100 to -20 mV) before and 10 minutes 10 after the addition of leptin (100 nM). GABAergic IPSCs were recorded using a CsCl internal electrode solution (in mM) [CsCl, 140; Hepes, 10; MgCl2, 5; Bapta, 1; (Mg)-ATP, 5; (Na)GTP, 0.3]. Both mini IPSCs and large amplitude (presumably multisynaptic) IPSCs were observed in the untreated slices. TTX (1 RM) abolished large IPSCs. Data were acquired before and after addition of drug for the times 15 indicated on the figures at a -50 mV holding potential in 2 s. sweeps every 4 s. Mini postsynaptic currents were analyzed using Axograph 4 (Axon Instruments). IPSCs and excitatory postsynaptic currents (EPSCs) were distinguished on the basis of their decay constants; additionally picrotoxin (100 pM) blocked all IPSCs. POMC neurons receive a low EPSC tone and the frequency was not modulated by any of 20 the treatments described here. Immunostainingfor light and electron microscopy: Double immunocytochemistry for NPY and POMC using different color diaminobenzidine(DAB) chromogens was carried out on fixed mouse hypothalami 25 according to published protocols (Horvath et al., Neuroscience 51, 391-9, 1992). For electron microscopy, preembedding immunostaining for p-endorphin was using an ABC Elite kit (Vector Laboratories) and a DAB reaction followed by post embedding labeling of GABA and NPY using rabbit anti-GABA, 1:1000 v/v and gold conjugated (10 nm) goat anti-rabbit IgG or sheep anti-NPY and gold 30 conjugated (25 nm) goat anti-sheep IgG. Finally, sections were contrasted with saturated uranyl acetate (10 minutes) and lead citrate (20-30 s) and examined using a Philips CM-10 electron microscope.

-101 Animals: Male Wistar rats (200-250g), 7-8 weeks old (Charles River Laboratories, United Kingdom) were maintained under controlled temperature (21 23' C) and light conditions (lights on 07:00-19:00) with ad libitum access to water and food (RMI dict; SDS Ltd., Witham, United Kingdom) except where stated. 5 Arcuate and paraventricular nuclei cannulations and injections were performed as previously described (Glaum et al., Mol. Pharmacol. 50, 230-5, 1996; Lee et al., J. Physiol (Lond) 515, 439-52; 1999; Shiraishi et al., Nutrition 15, 576-9, 1999). Correct intranuclear cannula placement was confirmed histologically at the end of each study period (Glaum et al., Mol. Pharmacol 50, 230-5, 1996; Lee et al., J. 10 Physiol (Lond) 515, 439-52, 1999; Shiraishi et al., Nutrition 15, 576-9, 1999). All animal procedures were approved under the British Home Office Animals (Scientific Procedures) Act, 1986. All injection studies on fasting animals were performed in the early light-phase (0800-0900). All dark-phase feeding studies injections were performed just prior to lights off. 15 Male Pomc-EGFP mice were studied at 5-6 weeks of age and were generated as described above. Y2r-null mice were generated using Cre-lox P mediated recombination, which results in the germline deletion of the entire coding region of the Y2 receptor. All Y2r-null mice were maintained on a mixed C57/B16 20 129SvJ background. Male mice aged 8-12 weeks and between 20-30 g bodyweight were kept under controlled temperature (21-23 C) and light conditions (lights on 06:00-18:00) with ad libitum access to water and food (Gordon's Speciality Stock feeds) except where stated. All studies were performed in the early light-phase (0700-0800). 25 Intraperitoneal injections: Rats were accustomed to IP injection by injections of 0.5 ml saline on the two days prior to study. For all studies, animals received an IP injection of PYY3.

36 , GLP-1 or saline in 500 VI (for rats) or 100 pl (for mice). 30 -102 Electrophysiology: Whole cell patch clamp recordings were made from POMC neurons in the hypothalamus of 180 jpm thick coronal slices from Pomc EGFP mice, as previously reported (Cowley et al., Nature 411, 480-484, 2001). "Loose cell-attached" recordings were made using extracellular buffer in the 5 electrode solution, and maintaining seal resistance between 3-5Mohm throughout the recording. Firing rates were analysed using mini-analysis protocols (MiniAnalysis, Jaejin Software, NJ). Vehicle controls were used in this system, previously validated for the electrophysiological actions of neuropeptides (Cowley et al., Nature 411, 480-484, 2001). Data were analysed by ANOVA, Neuman-Keuls 10 posthoc comparison, and Wilcoxon Signed Rank Test. Hypothalamic explants: Male Wistar rats were killed by decapitation and the whole brain immediately removed, mounted with the ventral surface uppermost and placed in a vibrating niicrotome (Biorad, Microfield Scientific Ltd., Devon, UK). A 15 1.7 rum slice was taken from the base of the brain to include the PVN and the ARC and immediately transferred to lml of artificial CSF (aCSF) (Kim et al., J. Clin. Invest. 105, 1005-11, 2000) equilibrated with 95% 02 and 5% CO 2 and maintained at 37* C. After an initial 2-hour equilibration period, with aCSF replaced every 60 minutes, the hypothalami were then incubated for 45 minutes in 600jl aCSF (basal 20 period) before being exposed to the Y2A (50nM) in 600 l aCSF. Finally, the viability of the tissue was verified by a 45 minute exposure to 56 mM KCL; isotonicity was maintained by substituting K* for Na *. At the end of each period, the aCSF was removed and frozen at -20* C until assayed for NPY and aMSH by radioimmunoassay. 25 C-fos expression: C-fos expression was measured in adult Wistar rats and Pomc-EGFP mice 2 hours after IP administration of saline or PYY 3

.

36 (5 jig/1 00g) using standard immunohistochemical techniques (Hoffman et al., Front. Neuroendocrinol 14, 173-213, 1993). Data were obtained from 3 rats and 5 mice in 30 each group. For the Pomc-EGFP mice 5 anatomically matched arcuate nucleus sections (Franldin et al., The Mouse Brain in Stereotaxic Coordinates, Academic -103 Press, San Diego, 1997) were counted from each animal, and images acquired using a Leica TSC confocal microscope (Grove et al., Neuroscience 100, 731-40, 2000). RNase protection assay (RPA): Total RNA was extracted from hypothalami 5 (Trizol, Gibco). RPAs were performed (RPAIII kit, Ambion) using 5Rg RNA and probes specific for NPY, aMSH and 0 actin (internal standard). For each neuropeptide, the ratio of the optical density of the neuropeptide mRNA band to that of P actin was calculated. Neuropeptide mRNA expression levels are expressed relative to saline control (mean ± s.e.m. n = 4 per group). The statistical analysis 10 used was ANOVA, with Bonferroni post hoc analysis. Plasma assays: Human leptin was measured using a commercially available radioimrnmunoassay (RIA) (Linco Research, USA). All other plasma hormone levels were measured using established in-house RIAs (Tarling et al., Intensive Care Med. 15 23, 256-260, 1997). Glucose concentrations were measured using a YSI 2300STAT analyser (Yellow Springs Instruments Inc., Ohio, USA). Plasma paracetamol levels were measured using an enzymatic colorimetric assay (Olympus AU600 analyzer). Human Studies: PYY 3 .3, was purchased from Bachem (California, USA). 20 The Limulus Amoebocyte Lysate assay test for pyrogen was negative and the peptide was sterile on culture. Ethical approval was obtained from the Local Research Ethics Committee (project registration 2001/6094) and the study was performed in accordance with the principles of the Declaration of Helsinki. Subjects gave informed written consent. 25 Each subject was studied on two occasions with at least 1 week between each study. Volunteers filled out a food diary for three days prior to each infusion, and for the following 24 hours. All subjects fasted and drank only water from 20:00 on the evening prior to each study. Subjects arrived at 08:30 on each study day, were cannulated and then allowed to relax for 30 minutes prior to the onset of the study 30 protocol. Blood samples were collected every 30 minutes into heparinised tubes containing 5,000 Kallikrein Inhibitor Units (0.2 ml) of aprotinin (Bayer) and centrifuged. Plasma was separated and then stored at -70' C until analysis. Subjects -104 were infused with either saline or 0.8 pmol.kg 1.min- PYY 3

.

36 for 90 minutes (about 72 pmol total infusion), in a double blind randomized crossover design. Two hours after the termination of the infusion, subjects were offered an excess free-choice buffet meal (Edwards et al., Am. J. Physiol. Endocrinol. Metab. 5 281, E155-E166, 2001), such that all appetites could be satisfied. Food and water were weighed pre- and postprandially and caloric intake calculated. Appetite ratings were made on 100 mm visual analogue scores (VAS) with the text expressing the most positive and the negative rating anchored at each end (Raben et al., Br. J. Nutr. 73, 517-30, 1995). VAS was used to assess hunger, satiety, fullness, prospective 10 food consumption and nausea. Caloric intake following saline and PYY 3

.

36 were compared using a paired t test. The postprandial response curves were compared by ANOVA using repeated paired measures, with time and treatment as factors. Measurements ofEnergy Expenditure: To determine the actions of PYY on 15 energy expenditure the OXYMAX system is utilized with rodents following PYY injection into a treatment cohort. This system is also utilized with rodents following a saline injection (control cohort). The equipment measures 02 consumption and

CO

2 production; the efficiency with which the body produces CO 2 from 02 gives a reliable index of caloric or metabolic efficiency. A similar system is used with 20 human volunteers.

-105 Example 2 Neural Network in the Arcuate Nucleus A strain of transgenic mice was generated expressing green fluorescent protein (EGFP Clontech), under the transcriptional control of mouse Pomc genomic 5 sequences that include a region located between -13 kb and -2 kb required for accurate neuronal expression (Young et al., JNeurosci 18, 6631-40, 1998) (Fig. la). Bright green fluorescence (509 nm) was seen in the two CNS regions where POMC is produced: the ARC and the nucleus of the solitary tract. Under ultraviolet (450 480 nm) excitation POMC neurons were clearly distinguished from adjacent, non 10 fluorescent neurons (Fig. lb) visualized under infrared optics. Double immunofluorescence revealed >99% cellular co-localization of EGFP and POMC peptides within the ARC (Fig. Ic). There was close apposition of both tyrosine hydroxylase (TH)- and NPY-stained terminals on EGFP-expressing POMC neurons, but no evidence of co-localization of the TH or NPY immunoreactivity with EGFP. 15 Total fluorescent cell counts performed on coronal hypothalamic sections revealed 3148 ± 62 (mean - SEM: n=3) POMC-EGFP neurons distributed through the entire ARC (Franklin et al., The Mouse Brain in Stereotaxic Coordinates, Academic Press, San Diego, 1997) (Fig. Id). POMC neurons in the mouse are located both medially and ventrally within the ARC, in contrast to a predominantly lateral position in the 20 rat ARC. POMC-EGFP neurons in hypothalamic slices had a resting membrane potential of-40 to -45 mV and exhibited frequent spontaneous action potentials. The non-selective opioid agonist met-enkephalin (Met-Enk: 30 PM; Sigma) caused a rapid (35- 40 s), reversible hyperpolarization (10-20 mV) of the membrane potential 25 of POMC cells (n=10) and prevented spontaneous action potential generation (Fig. 2a). In normal (2.5 mM K*) Krebs buffer, the reversal-potential of the inwardly rectifying opioid current was approximately -9OmV, while in 6.5 mM K Krebs the reversal-potential was shifted to approximately -60 mV (n=3: Fig. 2b). The p opioid receptor (MOP-R) antagonist CTAP (1 pM; Phoenix Pharmaceuticals) 30 completely prevented the current induced by Met-Enk in POMC cells (n=3: Fig. 2c). These characteristics indicate the opioid current was due to activation of MOP-R and increased ion conductance through G protein coupled, inwardly-rectifying -106 potassium channels (GIRK) (Kelly et al., Neuroendocrinology 52, 268-75, 1990). The similar opioid responses in EGFP-labeled POMC neurons to that of a guinea pig (Kelly et al., Neuroendocrinology 52, 268-75, 1990) or mouse (Slugg et al., Neuroendocrinology 72, 208-17, 2000). POMC cells, identified by post-recording 5 immunohistochemistry, suggests that expression of the EGFP transgene does not compromise either expression of receptors nor their coupling to second messenger systems in POMC neurons. Next, the direct effects of leptin on identified POMC cells in slice preparations were investigated. Leptin (0.1 - 100 nM) depolarized 72 of 77 POMC 10 cells by 3-30 mV (Fig. 3a; mean ± SEM depolarization at 100 nM leptin = 9.7 ± 1.2 mV, n= 45) within 2-10 minutes, in a concentration responsive manner (Fig. 3b). There were two components to the depolarization and neither were fully reversible within 40 minutes. Firstly, the depolarization was due to a small inward current which reversed at approximately -20 mV (Fig. 3c), suggesting the involvement of a 15 non-specific cation channel (Powis et al., Am JPhysiol 274, R1468-72, 1998). Secondly, leptin treatment decreased the GABAergic tone onto POMC cells. GABAergic inhibitory postsynaptic currents (IPSCs) were observed in.POMC cells and leptin (100 nM) decreased their frequency by 25% (Fig. 3d) in 5 out of 15 cells suggesting that it acted presynaptically to reduce GABA release (leptin had no effect 20 on IPSCs inl0 out of 15 POMC neurons). The effect on IPSC frequency occurred with a similar lag to the effect on membrane potential. Thus, leptin not only directly depolarizes POMC neurons but also acts at GABAergic nerve terminals to reduce the release of GABA onto POMC neurons, allowing them to adopt a more depolarized resting potential. The consistent depolarization of POMC cells by leptin 25 was specific because leptin had no effect on 5 of 13 adjacent non-fluorescent cells tested (Fig. 3e), while it hyperpolarized 5 (Fig. 3f) and depolarized 3 other non POMC neurons in the ARC. The electrophysiological effects of leptin reported here are consistent with leptin's biological actions; leptin rapidly causes release of a MSH from rat hypothalami (Kim et al., J Clin Invest 105, 1005-11, 2000), 30 presumably by activating POMC neurons. Previous reports of neuronal hyperpolarization by leptin (Glaum et al., Mol Pharmacol 50, 230-5, 1996; Spanswick et al., Nature 390, 521-5, 1997), and the -107 demonstrated co-localization of GABA and NPY (Horvath ct al., Brain Res 756, 283-6, 1997) within subpopulations of ARC neurons, led us to speculate that leptin hyperpolarizes NPY/GABA cells that directly innervate POMC neurons, and thus reduces GABAergic drive onto POMC cells. Both the leptin and NPY Y2 receptors 5 are expressed on NPY neurons in the ARC (Hakansson et al., JNeurosci 18, 559-72, 1998; Broberger et al., Neuroendocrinology 66, 393-408, 1997). Furthermore, activation of Y2 receptors inhibits NPY release from NPY neurons (King et al., J Neurochem 73, 641-6, 1999), and presumably would also diminish GABA release from NPY/GABA terminals. This is an alternative pharmacological approach, 10 independent of leptin, to test the hypothesized innervation of POMC neurons by GABAergic NPY neurons. Indeed, NPY (100 nM; Bachem) decreased the frequency of GABAergic IPSCs by 55% within 3 minutes, in all 12 POMC cells tested (Fig. 4a). Both NPY and leptin still inhibited IPSCs in the presence of tetrodotoxin (TTX) (6 of 6 and 3 of 5 cells respectively), indicating that some of the 15 inhibition of IPSCs was occurring through direct effects at presynaptic nerve terminals. POMC neurons express the NPY Y1 receptor (Broberger et al., Neuroendocrinology 66, 393-408, 1997) and NPY also hyperpolarized all POMC neurons tested, by an average of 9±6 mV (n=3). Another pharmacological test to confirm the origin of GABAergic 20 innervation on POMC neurons from NPY/GABA terminals was to test the effect of the recently characterized and highly selective MC3-R agonist D-Trp 8 'MSH (Grieco et al., JMed Chen 43, 4998-5002, 2000) on local GABA release. D-Trp -yMSH (7 nM) increased the frequency of GABAergic IPSCs (280 ± 90%) recorded from 3 of 4 POMC neurons (Fig. 4b). It had no effect on one cell. The positive 25 effect of MC3-R activation, together with the negative effects of NPY and leptin, demonstrate the dynamic range of the NPY/GABA synapse onto POMC neurons and point to the important role of this synapse in modulating signal flow within the ARC. D-Trp 8 -'yMSH (7 nM) also hyperpolarized (-5.5 ± 2.4 mV) 9 of 15 POMC neurons tested and decreased the frequency of action potentials (Fig 4c); the 30 remaining cells showed no significant response to D-Trp-'MSH. These effects could be due entirely to increased GABA release onto the POMC cells, or could be due to an additional postsynaptic action of D-Trps--yMSH on POMC neurons, -108 approximately half of which also express the MC3-R (Bagnol et al., JNeurosci (Online) 19, RC26, 1999). Thus, MC3-R acts in a similar autoreceptor manner to MOP-Rs on POMC neurons, diminishing POMC neuronal activity in response to elevated POMC peptides. 5 To further determine that the IPSCs in POMC neurons were due to local innervation by NPY/GABA cells, multi-label immunohistochemistry was performed using light and electron microscopy. Although independent NPY (Csiffary et al., Brain Res 506, 215-22, 1990) and GABA (Horvath et al., Neuroscience 51, 391-9, 1992) innervation of POMC cells has been reported, co-localization of NPY and 10 GABA in nerve terminals forming synapses onto POMC cells has not been shown. Similar to the rat (Csiffary et al., Brain Res 506, 215-22, 1990), a dense innervation of POMC cells by NPY axon terminals was detected in the mouse (Fig. 4d). Electron microscopy confirmed the coexpression of NPY and GABA in axon terminals and revealed that these boutons established synapses on the perikarya of 15 all 15 ARC POMC neurons analyzed (representative exampic, Fig. 4e). A detailed model of regulation of this circuit shows dual mechanisms of leptin action in the ARC, interactions between NPY/GABA and POMC neurons, and autoregulatory feedback from opioid and melanocortin peptides as well as NPY (Fig. 4f). In this model, leptin directly depolarizes the POMC neurons and 20 simultaneously hyperpolarizes the somata of NPY/GABA neurons, and diminishes release from NPY/GABA terminals. This diminished GABA release disinhibits the POMC neurons, and result in an activation of POMC neurons and an increased frequency of action potentials. 25 Example 3 Administration of PYY Inhibits Food Intake The orexigenic NPY and the anorectic alpha melanocortin stimulating hormone (a-MSH) systems of the hypothalamic arcuate nucleus are involved in the central regulation of appetite (Schwartz et al., Nature 404, 661-671, 2000). 30 However the potential mechanisms signaling meal ingestion directly to these hypothalamic-feeding circuits are unclear. PYY 3

.

36 is a gut-derived hormone that is released postprandially in proportion to the calories ingested (Pedersen-Bjergaard et -109 al., Scand. J. Clin. Lab. Invest. 56, 497-503, 1996). The effects of peripheral administration of PYY 3

.

36 on feeding were investigated. An intraperitoneal injection (IP) of PYY 3

.

36 to freely feeding rats, prior to the onset of the dark-phase, significantly decreased subsequent food intake (Fig. 5a). A 5 similar inhibition of feeding was seen following IP injection in rats fasted for 24 hours (Fig. 5b). A time course of the plasma PYY 3

.

3 6 levels achieved following IP injection of PYY 3 -3 6 demonstrated a peak level at 15 minutes post injection, which was within the normal postprandial range (peak PYY 3

-

36 levels 15 minutes post IP injection of 0.3pg/100g = 99.3 ± 10.4 pmol/l vs. peak postprandial level= 112.1 A 10 7.8 pmol/l, n = 8-10 per group), suggesting that physiological concentrations of

PYY

3

.

36 inhibit feeding. PYY 3

.

36 did not affect gastric emptying (percentage of food ingested remaining in the stomach at 3 hours: PYY 3

-

36 = 36 ± 1.9 %, saline = 37.41 1.0 % n = 12 ) (Barrachina et al., Am. J. Physiol. 272, R1007-11, 1997). PYY 3 -3 6 administered IP twice daily for 7 days reduced cumulative food intake (7-day 15 cumulative food intake: PYY 3

.

36 = 187.6 ± 2.7g vs. saline = 206.8 ± 2.3, n = 8 per group, P <0.0001) and decreased body weight gain (Fig. 5d) (PYY 3

.

36 = 48.2 ± 1.3g vs. saline = 58.7 ± 1.9, n = 8 per group, P <0.002). Example 4 20 PYY Administration Affects c-fos Expression To investigate whether this inhibition of food intake involved a hypothalamic pathway, c-fos expression was examined in the arcuate nucleus, an important center of feeding control (Schwartz et al., Nature 404, 661-671, 2000; Cowley et al., Nature 411, 480-484, 2001), following a single IP injection of PYY 3

.

3 6 . There was a 25 2-fold increase in the number of cells positive for c-fos in the lateral arcuate of the rat (PYY 3

.

36 =168 ± 2, saline = 82.7 ± 5, n= 3, P <0.0001). Likewise in Pomc EGFP-transgenic mice (Cowley et al., Nature 411, 480-484, 2001) IP administration of PYY 3 -3 6 resulted in a 1.8-fold increase in the number of arcuate cells positive for c-fos (Fig. 6b), compared with saline control animals (Fig. 6a) (PYY 3 .3 = 250 ± 40, 30 saline = 137 A 15, n = 5, P < 0.05). IP PYY 3 -3 6 caused a 2.6 fold increase in the proportion of POMC neurons that express c-fos (PYY 3

.

36 =20.4 ±2.9%, saline = 8 1.4%, n = 5, P <0.006) (Figs. 6c and d).

-110 These observations suggested that PYY 3

.

36 may act via the arcuate nucleus. Thus, the actions of PYY 3

.

36 , and its effects upon NPY and POMC circuits in the hypothalamus, were studied. In view of the sustained inhibition of food intake and the effects on weight gain following peripheral administration of PYY 3

-

36 both PoInc 5 and Npy hypothalamic messenger RNA (mRNA) were measured using RNase protection assays. A significant decrease in Npy mRNA in response to PYY 3 .3 6 was observed 6 hours post IP injection, compared with saline treated animals (saline = 17.3 + 2.0, PYY 3

.

3 6 = 8.8 + 1.0, relative optical density units, P <0.02). A non significant increase occurred in Pomc mRNA levels. 10 Example 5 Y2 receptors

PYY

3 .a 6 shows a 70% amino acid sequence identity to NPY and acts through NPY receptors (Soderberg et al., J. Neurochem. 75, 908-18, 2000). The Y2R is a 15 putative inhibitory presynaptic receptor and is highly expressed on the arcuate NPY neurons (Broberger et al., Neuroendocrinology 66, 393-408, 1997), though not on the neighboring POMC neurons. PYY 3

.

36 is a high affinity agonist at the Y2 receptor (Grandt et al., Regul. Pept. 51, 151-159, 1994). It was hypothesized that peripheral PYY 3

.

3 6 inhibits food intake via the Y2R in the arcuate nucleus, an area 20 known to be directly accessible to circulating hormones (Kalra et al., Endocr. Rev. 20, 68-100, 1999). To investigate this hypothesis, PYY 3 -36 was injected directly into the arcuate nucleus (Kim et al., Diabetes 49, 177-82, 2000). In rats fasted for 24 hours, food intake was significantly decreased by doses as low as 100 fmol (Fig. 7a), resulting in 25 a similar inhibition to that seen following IP administration. To establish whether these effects were via the Y2R, aY2R selective agonist was used (Potter et al., Eur. J. Pharmacol. 267, 253-262, 1994), N-acetyl (Leu 28, Leu ") NPY (24-36) [Y2A]. Its affinity was confirmed using receptor-binding studies (Small et al., Proc. Nat!. Acad. Sci. USA. 94, 11686-91, 1997) on cell lines expressing the NPY Yl, Y2 and 30 Y5 receptors (Y2 IC 50 = 1.3 ± 0.2 nM, Yl IC 5 0 > 5000 nM, Y5 IC 5 o> 5000 nM). Intra-arcuate nucleus injection of Y2A in rats previously fasted for 24 hours dose dependently (100 finol - 1 nmol) inhibited food intake (chow ingested 2 hours post- -111 injection, 0.1 nmol Y2A = 6.2 ± 0.5 g, saline = 8.2 ± 0.6 g, n = 8 per group, P < 0.05). To confirm the anatomical specificity of this effect Y2A (100 fmol - I nmol) was injected into the paraventricular nucleus (PVN) (Kim et al., J. Clin. Invest. 105, 5 1005-11, 2000) of rats fasted for 24 hours and found no alteration of food intake (2 hour post-injection saline = 8.3 -10.4 g, 0. inmol Y2A = 8.0 i 0.6 g, n = 8 per group). To further determine the role of the Y2R in the feeding inhibition caused by peripheral PYY 3

.

36 , the effect of PYY 3

.

3 6 on Y2r-null mice and littermate controls was examined. PYY 3

.

3 6 inhibited daytime feeding in a dose responsive manner in 10 fasted male wild-type mice but did not inhibit food intake in fasted male Y2r-null mice (Figs. 7b and 7c). Food intake measured in response to a fast demonstrated that male Y2r-null mice eat significantly more at 2, 4 and 24 hours compared with their littermate controls (24-hour cumulative food intake; Y2r-null mice = 7.1 0.48g vs. wild-type = 5.3 ± 0.7g, n = 8 per group, P < 0.05). 15 The electrophysiological response of hypothalamic P0MC neurons to administration of both PYY3.3 6 and Y2A was examined. These neurons werc identified using mice with targeted expression of green fluorescent protein in POMC neurons (Cowley et al., Nature 411, 480-484, 2001). PYY 3

.

36 disinhibited the POMC neurons, resulting in a significant depolarization of 19 of the 22 POMC 20 neurons tested (Fig. 8a inset) (10.3 ± 2.1 mV depolarization, n = 22, P <0.0003). A similar depolarization was seen with Y2A (8.7 * 1.8 mV depolarization, n = 9, P < 0.002). The depolarization caused by PYY 3 .36 stimulated a significant increase in the frequency of action potentials in POMC neurons (Fig 8a) (93% increase over control, P < 0.05, n = 22). In the whole cell mode the effect of PYY 3

-

36 was 25 sometimes reversed upon washout, but only after a long latency (30 minutes). A similar washout of leptin effects upon these neurons was observed. To exclude effects of cellular rundown, or seal deterioration, the effects of

PYY

3

-

36 in the "loose cell-attached" (or extracellular) configuration was examined. PYY3.

3 6 caused a reversible 5-fold increase in the frequency of action potentials in 30 loose cell-attached recordings of POMC neurons (Fig. 8b). This increase in firing rate occurred with the same latency as PYY 3

.

36 reduced the frequency of inhibitory postsynaptic currents (IPSCs) onto all 13 POMC neurons tested (Fig. 8c) (51.9 i 9.2 -. 112 % reduction, n = 13, P < 0.0001), indicating a reduced frequency of GABA release onto POMC neurons. Interestingly, the firing rate of POMC neurons returned to basal, in spite of continued inhibition of IPSCs. A similar effect upon IPSC frequency was seen with Y2A (44.4 ± 9.3% reduction, n = 8, P < 0.004) suggesting 5 this effect to be via Y2R. PYY 3 .36 (25 nM) caused a hyperpolarization (5.2 ± 1.16 mV, P < 0.004, n = 5) of unidentified, but presumably NPY-containing, non-POMC, neurons in the arcuate nucleus. There is a tonic GABAergic inhibition of POMC neurons by NPY neurons (Cowlcy et al., Nature 411, 480-484, 2001) and these results suggest that PYY 3

.

36 acts by inhibiting NPY neurons, thus decreasing this 10 GABAergic tone and consequentially disinhibiting POMC neurons. The effect of Y2A on peptide secretion was also examined using hypothalamic explants (Kim et al., J. ClIn. Invest. 105, 1005-11, 2000). Y2A significantly decreased NPY release, with a concomitant increase in oi-MSH release from hypothalamic explants (Figs. 8d and 4e). Taken together, these observations suggest that PYY 3

.

36 modulates both the 15 NPY and melanocortin systems in the arcuate nucleus. Example 6 Human Studies Because of the importance of the melanocortin system in man (Barsh et al., 20 Nature 404, 644-651, 2000) and the profound effects of PYY 3

-

36 on both feeding and weight change seen in rodents, the effects of PYY 3

-

36 on appetite and food intake were investigated in human subjects. Twelve healthy fasted, non-obese volunteers (six men and six women, mean age 26.7 10.7 years, BMI= 24.6 -10.94 kg.m- 2 ) were infused with PYY 3

-

36 (0.8 pmol.kg- .min') or saline for 90 minutes in a 25 double-blind placebo controlled crossover study.

PYY

3

.

36 plasma concentrations increased from mean basal concentration of 8.3 ± 1.0 pM to 43.5 ± 3 pM during the PYY 3

-

36 infusion and mimicked postprandial levels (Pedersen-Bjergaard et al., Scand. J Clin. Lab. Invest. 56, 497-503, 1996; Adrian et al., Gastroenterology 89, 1070-1077, 1985). Post-infusion, PYY 3

.

36 30 concentrations returned to basal within 30 minutes. PYY 3

.

3 6 infusion resulted in a significant decrease in hunger scores (Raben et al., Br. J. Nutr. 73, 517-30, 1995) (Fig. 9c), but not in the scores for sleepiness or sickness. Calorie intake during a -113 free-choice buffet meal (Tarling et al., Intensive Care Med. 23, 256-260, 1997) two hours after the termination of the infusion was reduced by over a third compared to saline (36 t 7.4%, p < 0.0001) (Fig. 9a). There was no effect upon fluid intake and no difference in sensations of fullness or nausea reported by the volunteers. PYY 3 .36 5 administration had no effect on gastric emptying, as estimated by the paracetamol absorption method (Edwards et al., Am. J. Physiol. Endocrinol. Metab. 281, E155 El 66, 2001; Tarling et al., Intensive Care Med. 23, 256-260, 1997), or on plasma glucose, plasma leptin, GLP-1, or insulin. Analysis of the food diaries revealed a significant inhibition of food intake in the 12-hour period following the PYY 3

.

36 10 infusion (saline = 2205 + 243 kcal, PYY 3

-

36 = 1474 ± 207 kcal). However, food intake during a 12 to 24 hour period between the two groups was virtually identical. Overall there was a 33% decrease in cumulative total calorie consumption in the 24 hour period following the PYY 3

.

3 6 infusion (Fig.9b). These findings demonstrate that infusion of PYY 3

.

36 , matching postprandial levels, caused a marked inhibition of 15 both appetite and food intake in man. In an additional study, two groups of healthy subjects (n = 12 per group, 6 males and 6 females), one with increased Body Mass Index (BMI) ( mean = 32.73 +/- 0.93 kg/m2) and another group with low BMI (mean = 20.49 +/- 2.05 kg/m2), were studied on two occasions with at least 1 week between each study. All subjects 20 fasted and drank only water from 20:00 hours on the evening prior to each study. Subjects arrived at 08:30 on each study day, were cannulated and then allowed to relax for 30 minutes prior to the onset of the study protocol. Subjects were infused with either saline or 0.8 pmol.kgl.min-I PYY 3

-

36 for 90 minutes, in a double blind randomized crossover design. Two hours after the termination of the infusion, 25 subjects were offered an excess free-choice buffet meal, such that all appetites could be satisfied. Food and water were weighed pre- and postprandially and caloric intake calculated. Caloric intake following saline and PYY 3

.

36 were compared using a paired t test ( p<0.001). The number of calories ingested following administration of PYY 3

.

36 differed significantly from the number of calories ingested following 30 administration of saline for both the overweight group and the lean group. The overweight group showed a 28.8 +/- 4.3 % reduction and the lean group a 31.1+/ 4.4 % reduction. However, the reduction for the overweight group did not differ -114 significantly from the reduction for the lean group. These findings demonstrate that infusion of PYY 3

.

36 , matching postprandial levels, caused a marked inhibition of both appetite and food intake in both lean and overweight subjects. Administration of PYY 3

.

3 6 at doses of 10 nmoles and 20 moles (in saline) 5 subcutaneously to human subjects resulted in raised plasma levels of PYY 3 .a 6 as shown in Fig. 10. Without being bound by theory, cells within the arcuate nucleus could detect circulating peripheral satiety signals and relay these signals to other brain regions (Butler et al., Nature Neuroscience 4, 605-611, 2001). This is supported by the 10 observation that leptin modifies the activity of both the POMC and NPY arcuate neurons (Cowley et al., Nature 411, 480-484, 2001). The results disclosed herein demonstrate, through a combination of electrophysiological and hypothalamic explant studies, that the gut hormone, PYY 3

.

36 , can directly influence hypothalamic circuits, resulting in coordinate changes in POMC and NPY action. The results 15 presented here demonstrate that NPY neurons in the ARC are not protected by the blood/brain barrier, and thus are accessible to circulating molecules. Furthermore, PYY3.

36 administered directly into this brain region reduces food intake. The data disclosed herein demonstrates that postprandial levels of PYY 3

.

36 inhibit food intake in more than one mammalian species (e.g. rodents and human 20 subjects) for up to 12 hours, thereby demonstrating a role in regulation of food intake. This role can be described as a long term role, such as over a period of several hours (e.g. at least two, three, four, eight, or twelve hours, or from about two to about fifteen hours). This is in contrast to previously characterized gut-derived 'short-term' satiety signals, e.g. cholecystokinin (Schwartz et al., Nature 404, 661 25 671, 2000; Moran, Nutrition 16, 858- 865, 2000), the effects of which are relatively short-lived (e.g., from about 1-4 hours). The failure of PYY 3

.

36 to inhibit food intake in the Y2r-null mice provides evidence that PYY 3 -3 6 reduces food intake via a Y2R dependent mechanism. The results disclosed herein suggest the existence of a novel gut-hypothalamic pathway 30 in the regulation of feeding, involving postprandial PYY 3

.

36 acting at the arcuate Y2R. Thus, PYY, and analogs thereof, such as PYY 3

.

3 6 provide novel therapeutic agents for the treatment of obesity.

-115 Example 7 Parenterally administered GLP-1 5 GLP-1 i.e. GLP-1 (7-36) amide (25 nmol/kg) or saline (control) was administered parenterally to non-fasted rats prior to the onset of the dark phase. Exendin 9-39 (20 nmoles/kg) or saline (control) injected into the arcuate nucleus of non-fasted rats. Food intake one hour later was measured. The results as shown in Fig. 11 demonstrate that the effect on 1-hour food 10 intake in non-fasted rats of IP administration of GLP-1 is not influenced by the presence of concomitant exendin 9-39, a GLP-l receptor agonist, in the arcuate nucleus of the rat. This indicates that circulating GLP-1 acts via the brainstem rather than the arcuate nucleus. These and other findings suggest that the main site for appetite inhibition by peripheral GLP-1 is the dorsal vagal complex, acting in part 15 directly through the area postrema. Example 8 Co-administration of PYY 3

.

36 and GLP-1 in rats 20 PYY 3

.

36 and GLP-1 i.e. GLP-1 (7-36) amide were injected (intraperitoneally) into non-fasted rats prior to the onset of the dark phase. GLP- 1 and PYY 3

.

36 were administered separately at the following doses: GLP-1 30Lg/kg = 1xG or 60pug/kg 2xG in 500 pl saline; PYY 3

.

36 (3yig/kg = 1xP or 6pig/kg = 2xP in 500 yl saline).

PYY

3

.

36 and GLP-1 were co-administrated IP (P+G group) at doses of GLP-1 25 30pg/kg and PYY 3

.

36 3yg/kg combined. - The results set out in Fig. 12 show that there is a clear synergistic effect on the reduction of food intake when PYY 3

.

3 6 and GLP-1 were co-administered (P+G). However, when the procedure was carried out substituting oxyntomodulin for GLP 30 1, the results obtained for reduction of food intake when PYY 3

.

36 and -116 oxynotomdulin were co-administered were only the sum of the individual effects. No synergistic effect was observed. Example 9 5 Co-administration of PYY3.a 6 and GLP-1 in humans 10 healthy volunteers (4 men and 6 women) having a mean age of 24.7 (+/ 0.8) years and a mean BMI of 22.7 (+/- 0.6) kg/m 2 were recruited. The volunteers were asked to fast and to drink only water from 9 pm on the night preceding all 10 study days. Each volunteer attended for four study days. The volunteers arrived at 9.30 am and the venflons were inserted. A rest period from 10.00 to 10.30 followed. Intrvenous infusion started at 10.30, and a buffet lunch was provided between 12.00 and 12.30. Infusion was terminated at 12.30, and the volunteers went home at 13.30. The volunteers were given the following intra-venous infusions for 120 15 minutes (90 minutes pre-meal) in random order: Saline, GLP-1, PYY 3

-

36 , PYY 3 36 +GLP-1. The dose of both GLP-1 and PYY3-36 was 0.4pmol/kg/min. The study was double blinded. GLP-1 is i.e. GLP-1 (7-36) amide The results of the food intake from the free buffet meal are given in the Table below 20 Food intake from free buffet meal (Kcal) Saline G P P+G mean 997 950 848 724 Sem 68.0 81.3 105.2 66.6 p v saline 0.336318995 0.069375886 3.00605E-06 p v P+G 0.003622523 0.044418337 % reduction from saline 5% 15% 27% using paired t tests for all statistical comparisons 25 These result demonstrate a synergistic effect when PYY3-36 and GLP-1 are co-administered. The results obtained in Examples 4 and 5 above demonstrate that PYY 3

.

36 acts via the arcuate nucleus. Oxynotmodulin is also considered to act via that region -117 of the brain. The results obtained in Example 8 above indicates that GLP-1 acts via the brainstem. It appears from the results obtained in this Example that, when agents such as PYY 3

-

36 and GLP-1 that act via different areas of the brain and/or by different neurological routes are administered parenterally, they produce a 5 synergistic affect, whereas when they act by the same area and/or route such as

PYY

3 s 36 and oxynotmodulin, they do not. The synergistic effect on food intake obtained when PYY and GLP-1 were co-administered to humans confirms the observation made in rats. 10 These results indicate the importance in vivo of the interaction of gut hormones on the regulation of appetite and food intake. The present invention demonstrates that, surprisingly, it is possible to regulate appetite and food intake more effectively by informed choice of agents. 15 It will be apparent that the precise details of the methods or compositions described maybe varied or modified without departing from the spirit of the described disclosure. We claim all such modifications and variations that fall within the scope and spirit of the claims below.

Claims (57)

1. A method for decreasing calorie intake in a subject, a method for decreasing appetite in a subject, a method for decreasing food intake in a subject, a method for 5 weight control or treatment in a subject, or a method for reduction or prevention of obesity in a subject, which comprises peripherally administering a therapeutically effective amount of PYY or an agonist thereof and a therapeutically effective amount of GLP- I or an agonist thereof to the subject. 10

2. A method for preventing and reducing weight gain in a subject; a method for inducing and promoting weight loss in a subject; or a method for reducing obesity in a subject as measured by the Body Mass Index, which comprises peripherally administering a therapeutically effective amount of PYY or an agonist thereof and a therapeutically effective amount of GLP- I or an agonist thereof to the subject. 15

3. A method for controlling of any one or more of appetite, satiety and hunger in a subject, which comprises peripherally administering a therapeutically effective amount of PYY or an agonist thereof and a therapeutically effective amount of GLP 1 or an agonist thereof to the subject. 20

4. A method as claimed in claim 3 for inducing, increasing, enhancing or promoting satiety and/or sensations of satiety in a subject, which comprises peripherally administering a therapeutically effective amount of PYY or an agonist thereof and a therapeutically effective amount of GLP-1 or an agonist thereof to the 25 subject.

5. A method as claimed in claim 3 for reducing, inhibiting or suppressing hunger or sensations of hunger in a subject, which comprises peripherally administering a therapeutically effective amount of PYY or an agonist thereof and a 30 therapeutically effective amount of GLP-1 or an agonist thereof to the subject. -119

6. A method for maintaining desired body weight, a desired Body Mass Index, and/or a desired appearance and good health in a subject, which comprises peripherally administering a therapeutically effective amount of PYY or an agonist thereof and a therapeutically effective amount of GLP- I or an agonist thereof to the 5 subject.

7. A method for improving lipid profile in a subject, which comprises peripherally administering a therapeutically effective amount of PYY or an agonist thereof and a therapeutically effective amount of GLP-1 or an agonist thereof to the 10 subject.

8. A method for alleviating a condition or disorder which can be alleviated by reducing nutrient availability, which comprises peripherally administering a therapeutically effective amount of PYY or an agonist thereof and a therapeutically 15 effective amount of GLP-1 or an agonist thereof to the subject.

9. A method as claimed in any one of claims 1 to 8, wherein the PYY or agonist thereof and the GLP-1 or agonist thereof are administered simultaneously or sequentially. 20

10. A method as claimed in any one of claims I to 9, wherein the PYY and the GLP-1 are administered via different routes.

11. A method as claimed in any one of claims I to 10, wherein the subject is 25 overweight.

12. A method as claimed in any one of claims I to 11, wherein the subject is obese. ~ 30

13. A method as claimed in any one of claims 1 to 12, wherein the subject is diabetic. -120

14, A method as claimed in any one of claims I to 13, wherein peripheral administration comprises subcutaneous, intravenous, intramuscular, intranasal, transdermal or sublingual administration. 5

15. A method as claimed in any one of clams 1 to 14, wherein peripherally administering PYY or the agonist thereof comprises administering about 45 to about 135 pmol per kilogram body weight of the subject.

16. A method as claimed in claim 15, wherein peripherally administering PYY 10 or the agonist thereof comprises administering about 72 pmol per kilogram body weight of the subject.

17. A method as claimed in claim 15, wherein peripherally administering PYY or the agonist thereof comprises administering about 45 to about 135 pmol per 15 kilogram body weight of the subject at least 30 minutes prior to a meal.

18. A method as claimed in any one of claims I to 14, wherein peripherally administering the therapeutically effective amount of PYY or the agonist thereof comprises administering PYY or an agonist thereof to the subject in a multitude of 20 doses, wherein each dose in the multitude of doses comprises administration of about 0.5 to about 135 pmol per kilogram of body weight at least about 30 minutes prior to a meal.

19. A method as claimed in any one of claims 1 to 18, wherein the PYY or the 25 agonist thereof is administered in an amount sufficient to decrease calorie intake for a period of at least about 2 hours.

20. A method as claimed in any claim 19, wherein the PYY or the agonist thereof is administered in an amount sufficient to decrease calorie intake for a period 30 of about 2 to 12 hours.

21. A method as claimed in any one of claims I to 14, wherein the PYY or -121 agonist thereof is administered peripherally at a dose of 0.1 nmoles per kg body weight of the subject or more, for example, 0.2 nmoles or more, for example, 0.4 nmoles or more, for example, 0.6 nmoles or more, for example, 0.8 nmoles or more, for example, 1.0 nmole or more, for example, 1.2 moles or more, for example, 1.4 5 nmoles or more, for example, 1.6 nmoles or more, for example, 1.8 nmoles or more, for example, 2.0 moles or more, for example, 2.2 nmoles or more, for example, 2.4 moles or more, for example, 2.6 nmoles or more, for example, 2.8 nmoles, for example, 3.0 nmoles or more, for example, up to 3.2 nmoles per kg body weight. 10

22. A method as claimed in any one of claims I to 14 or claim 21, wherein the PYY or agonist thereof and/or the GLP- I or agonist thereof is administered peripherally in an amount of up to 3.0 mnoles per kg body weight, for example, up to 2.8 nmoles, for example, up to 2.6 moles, for example, up to 2.4 nmoles, for example, up to 2.2 moles, for example, up to 2.0 nmoles, for example, up to 1.8 15 nmoles, for example, up to 1.4 nmoles, for example, up to 1.2 moles, for example, up to 1.0 nmoles, for example, up to 0.8 nmoles, for example, up to 0.6 nmoles, for example, up to 0.4 moles, for example, up to 0.2 nmoles per kg body weight.

23. A method as claimed in any one of claims I to 14, 21 and 22, wherein the 20 GLP-1 or agonist thereof is administered peripherally at a dose of 0.1 moles per kg body weight of the subject or more, for exampic, 0.2 moles or more, for example, 0.4 nmoles or more, for example, 0.6 nmoles or more, for example, 0.8 nmoles or more, for example, 1.0 mole or more, for example, 1.2 moles or more, for example, 1.4 nmoles or more, for example, 1.6 moles or more, for example, 1.8 25 moles or more, for example, 2.0 nmoles or more, for example, 2.2 nmoles or more, for example, 2.4 nmoles or more, for example, 2.6 nmoles or more, for example, 2.8 moles, for example, 3.0 nmoles or more, for example, up to 3.2 nmoles per kg body weight. 30

24. A method as claimed in any one of claims I to 14 and 21 to 23, wherein the GLP- 1 or agonist thereof is administered peripherally in an amount of up to 3.0 rnmoles per kg body weight, for example, up to 2.8 nmoles, for example, up to 2.6 -122 nmoles, for example, up to 2.4 moles, for example, up to 2.2 nmoles, for example, up to 2.0 moles, for example, up to 1.8 moles, for example, up to 1.4 nmoles, for example, up to 1.2 nmoles, for example, up to 1.0 nmoles, for example, up to 0.8 nmoles, for example, up to 0.6 moles, for example, up to 0.4 moles, for example, 5 up to 0.2 nmoles per kg body weight.

25. A method as claimed in any one of claims I to 24, wherein the PYY agonist comprises a molecule that specifically binds the Y2 receptor. 10

26. A method as claimed in claim , wherein the PYY agonist increases the expression of c-fos in a section of an arcuate nucleus contacted with the compound.

27. A method as claimed in any one of claims I to 24, wherein the PYY agonist specifically binds to a neuropeptide Y neuron and inhibits an activity of a 15 neuropeptide Y neuron.

28. A method as claimed in claim 27, wherein the PYY agonist decreases the action potential firing rate of the neuropeptide Y neuron. 20

29. A method as claimed in claim 27, wherein the neuropeptide Y neuron synapses with a proopiomelanocortin neuron, and wherein binding of the PYY agonist to the neuropeptide Y neuron results in an increased activity of the proopiomelanocortin neuron. 25

30. A method as claimed in claim 27, wherein the decreased activity of the neuropeptide Y neuron results in an increase in action potential firing on the proopiomelanocortin neuron.

31. A method as claimed in any one of claims 1 to 30, wherein the GLP-1 30 agonist is exendin-4 or a derivative thereof that is a GLP- 1 agonist.

32. A method as claimed in any one of claims I to 31, further comprising -123 administering a therapeutically effective amount of amfepramone (diethylpropion), phentermine, mazindol, phenylpropanolamine, fenfluramine, dexfenfluramine, or fluoxetine 5

33. A method as claimed in any one of claims 1 to 32, wherein the subject is human.

34. Use of PYY and an agonist thereof and GLP- I or an agonist thereof for the manufacture of a medicament or medicaments for use in a method as defined in any 10 one of claims I to 33.

35. Use of PYY and an agonist thereof in the manufacture of a medicament for use, in combination with GLP-I or an agonist thereof, in a method for decreasing calorie intake in a subject, a method for decreasing appetite in a subject, a method 15 for decreasing food intake in a subject, a method for weight control or treatment in a subject, or a method for reduction or prevention of obesity in a subject.

36. Use of PYY and an agonist thereof in the manufacture of a medicament for use, in combination with GLP-1 or an agonist thereof, in a method for preventing 20 and reducing weight gain in a subject; a method for inducing and promoting weight loss in a subject; or a method for reducing obesity in a subject as measured by the Body Mass Index.

37. Use of PYY and an agonist thereof in the manufacture of a medicament for 25 use, in combination with GLP-1 or an agonist thereof, in a method for controlling of any one or more of appetite, satiety and hunger in a subject.

38. Use of PYYand an agonist thereof in the manufacture of a medicament for use, in combination with GLP-1 or an agonist thereof, in a method for inducing, 30 increasing, enhancing or promoting satiety and/or sensations of satiety in a subject.

39. Use of PYY and an agonist thereof in the manufacture of a medicament for -124 use, in combination with GLP-1 or an agonist thereof, in a method for reducing, inhibiting or suppressing hunger or sensations of hunger in a subject.

40. Use of PYY and an agonist thereof in the manufacture of a medicament for 5 use, in combination with GLP-1 or an agonist thereof, in a method for maintaining desired body weight, a desired Body Mass Index, and/or a desired appearance and good health in a subject.

41. Use of PYY and an agonist thereof in the manufacture of a medicament for 10 use, in combination with GLP- I or an agonist thereof, in a method for improving lipid profile in a subject

42. Use of PYY and an agonist thereof in the manufacture of a medicament for use, in combination with GLP-1 or an agonist thereof, in a method for alleviating a 15 condition or disorder which can be alleviated by reducing nutrient availability.

43. Use as claimed in any one of claims 31 to 41, wherein the PYY or agonist thereof and the GLP-1 or agonist thereof are administered simultaneously, or sequentially in either order. 20

44. A pharmaceutical composition comprising PYY and an agonist thereof and GLP-1 or an agonist thereof, in admixture or conjunction with a pharmaceutically suitable carrier. 25

45. A pharmaceutical composition comprising PYY or an agonist thereof in admixture with a pharmaceutically suitable carrier, in a form suitable for subcutaneous administration

46. A pharmaceutical composition as claimed in claim 45, which comprises 10 30 nmoles or more, for example, 20 nmoles or more, for example, 30 moles or more, for example, 40 moles or more of PYY or an agonist thereof. -125-

47. A pharmaceutical composition as claimed in claim 46, which comprises from 20 to 60 moles, for example, 35 to 45 moles of PYY or an agonist thereof.

48. A pharmaceutical composition as claimed in claim 45, which comprises from 5 PYY or an agonist thereof in an amount suitable for administration peripherally in an amount of up to 3.0 nmoles per kg body weight, for example, up to 2.8 nmoles, for example, up to 2.6 nmoles, for example, up to 2.4 nmoles, for example, up to 2.2 nmoles, for example, up to 2.0 moles, for example, up to 1.8 nmoles, for example, up to 1.4 nmoles, for example, up to 1.2 nmoles, for example, up to 1.0 moles, for 10 example, up to 0.8 nmoles, for example, up to 0.6 nmoles, for example, up to 0.4 nmoles, for example, up to 0.2 nmoles per kg body weight.

49. A pharmaceutical composition as claimed in claim 45, which comprises from PYY or an agonist thereof in an amount suitable for administration peripherally in 15 an amount of 0.1 moles per kg body weight of the subject or more, for example, 0.2 nmoles or more, for example, 0.4 moles or more, for example, 0.6 moles or more, for example, 0.8 nmoles or more, for example, 1.0 nmole or more, for example, 1.2 nmoles or more, for example, 1.4 nmoles or more, for example, 1.6 moles or more, for example, 1.8 nmoles or more, for example, 2.0 nmoles or more, 20 for example, 2.2 nmoles or more, for example, 2.4 moles or more, for example, 2.6 nmoles or more, for example, 2.8 nmoles, for example, 3.0 moles or more, for example, up to 3.2 roles per kg body weight.

50. A pharmaceutical composition as claimed in claim 48 or 49, in unit dosage 25 form.

51. A method as defined in any one of claims I to 8, which comprises administering PYY or an agonist thereof subcutaneously at a dose of 10 nmoles or more, for example, 20 nmoles or more, for example, 30 nmoles or more, for 30 example, 40 nmoles or more to the subject of the method.

52. A method as claimed in claim 51, wherein from 20 to 60 nmoles, for -126 example, 35 to 45 nmoles of PYY or an agonist thereof are adminisntered.

53. Use of PYY or an agonist thereof for the manufacture of a composition for subcutaneous administration to a subject for treatment of the subject by a method as 5 defined in any one of claims 1 to 8.

54. Use as claimed in claim 53, wherein the treatment comprises administering PYY or an agonist thereof subcutaneously at a dose of 10 nmoles or more, for example, 20 nmoles or more, for example, 30 moles or more, for example, 40 10 nmoles or more to the subject.

55. Use as claimed in claim 54, wherein the treatment comprises administering PYY or an agonist thereof subcutaneously at a dose of from 20 to 60 nmoles, for example, 35 to 45 nmoles to the subject. 15

56. Use as claimed in claim 53, wherein the PYY or agonist thereof is administered peripherally at a dose of 0.1 moles or more per kg body weight of the subject, for example, 0.2 nmoles or more, for example, 0.4 nmoles or more, for example, 0.6 nmoles or more, for example, 0.8 moles or more, for example, 1.0 20 nmole or more, for example, 1.2 moles or more, for example, 1.4 nmoles or more, for example, 1.6 nmoles or more, for example, 1.8 moles or more, for example, 2.0 moles or more, for example, 2.2 nmoles or more, for example, 2.4 nmoles or more, for example, 2.6 nmoles or more, for example, 2.8 moles, for example, 3.0 nmoles or more, for example, up to 3.2 nmoles per kg body weight. 25

57. Use as claimed in claim 53 or claim 56, wherein the PYY or agonist thereof is administered peripherally in an amount of up to 3.0 nmoles per kg body weight, for example, up to 2.8 nmoles, for example, up to 2.6 nmoles, for example, up to 2.4 nmoles, for example, up to 2.2 nmoles, for example, up to 2.0 nmoles, for example, 30 up to 1.8 moles, for example, up to 1.4 nmoles, for example, up to 1.2 nmoles, for example, up to 1.0 nmoles, for example, up to 0.8 moles, for example, up to 0.6 -127 nmoles, for example, up to 0.4 moles, for example, up to 0.2 nmoles per kg body weight. 5