AU2012201242B2 - Processes and intermediates for the preparation of aspartic acetal caspase inhibitors - Google Patents

Abstract

Processes and intermediates for the preparation of aspartic acetal caspase inhibitors Abstract The invention relates to processes such as that shown in the scheme and compounds useful for producing modified aspartic acid derivatives, such as aspartic acid aldehyde s moieties. Aspartic acid derivatives are useful for preparing caspase inhibitors and/or prodrugs thereof.

Description

S&F Ref: 779463D1 AUSTRALIA PATENTS ACT 1990 COMPLETE SPECIFICATION FOR A STANDARD PATENT Name and Address Vertex Pharmaceuticals Incorporated, of 130 Waverly of Applicant: Street, Cambridge, Massachusetts, 02139-4242, United States of America Actual Inventor(s): Andrew D. Jones David J. Guerin Gerald J. Tanoury Philip L. Nyce Martin Trudeau Minzhang Chen John R. Snoonian Address for Service: Spruson & Ferguson St Martins Tower Level 35 31 Market Street Sydney NSW 2000 (CCN 3710000177) Invention Title: Processes and intermediates for the preparation of aspartic acetal caspase inhibitors The following statement is a full description of this invention, including the best method of performing it known to me/us: 5845c(6070599_1) Processes and intermediates for the preparation of aspartic acetal caspase inhibitors [001] This application claims priority under 35 U.S.C. § 119(e) to U.S. Serial No. 60/552,480 filed on 12 March 2004, the entire contents of which are hereby incorporated by reference. FIELD OF THE INVENTION [0021 This invention relates to processes for preparing caspase inhibitors and intermediates derivatives thereof. BACKGOUND OF THE INVENTION [003] Caspases are a family of cysteine protease enzymes that are key mediators in the signaling pathways for apoptosis and cell disassembly (Thornberry, Chem. Biol., 1998, 5, R97-R103). Apoptosis, or programmed cell death, is a principal mechanism by which organisms eliminate unwanted cells. The deregulation of apoptosis, either excessive apoptosis or the failure to undergo it, has been implicated in a number of diseases such as cancer, acute inflammatory and autoimmune disorders, and certain neurodegenerative disorders (see generally Science, 1998, 281, 1283-1312; Ellis et al., Ann. Rev. Cell. Biol., 1991, 7, 663). Caspase-1, the first identified caspase, is also known as interleukin-1 converting enzyme or "ICE." Caspase-1 converts precursor interleukin-10 ("pIL-13") to the pro-inflammatory active form by specific cleavage of pIL-1P between Asp-116 and Ala-117. Besides caspase-1 there are also eleven other known human caspases which have been classified into families based on their biological function. [004] Many currently reported synthetic routes for producing caspase inhibitors require expensive starting materials, chromatographic separation of diastereomers, and/or disadvantageous synthetic steps.

-2 [005] It would be desirable to have a synthetic route to caspase inhibitors, or prodrugs thereof, that is amenable to large-scale synthesis and overcomes the aforementioned shortcomings or otherwise improves upon the current methods. SUMMARY OF THE INVENTION 5 [006] The processes and compounds described herein are useful for producing modified aspartic acid derivatives, such as aspartic acid aldehyde moieties. Aspartic acid derivatives are useful for preparing caspase inhibitors and/or prodrugs thereof. [007] Disclosed herein is a process for preparing a compound of formula GIA or GIB: O0 Ra OR4 0 GIA GIB 10 comprising the steps of: (a) reacting a compound of formula GIIA or GIIB: 0 Ra OR4 Ra _OR4 RaX X p~ H GI1A GIIB; and a compound of formula GIII: 0 R"

NH

2 15 GIII; in the presence of a palladium catalyst, a palladium ligand, and a base in a solvent optionally including a phase transfer catalyst and optionally including water; wherein: A1Tfl1 /A0hil 1 \.TTD -3 X is a leaving group; Ra is H, an optionally substituted alkyl, an optionally substituted aryl, -CN, -C(O)-Oalkyl or halogen;

R

3 is an organic moiety; 5 R2 is an optionally substituted alkyl, heterocyclic, alkylaryl, or aryl; and

R

4 is an optionally substituted aliphatic, a heterocyclic, or an aromatic; or R2 and R4 together with the groups to which they are bound, form a 5- to 8 membered heterocyclic ring which is optionally substituted. Embodiments of this aspect may include using a phase transfer catalyst. 10 [007a] Specifically, a first aspect of the present invention provides a process of producing a compound of 0

R

8 -N H N |0

O-R

9 15 comprising purifying a mixture of 0

R

8 -N H NI 0

O-R

9 wherein R 8 is a protecting group and R 9 is C 1 5 alkyl, and purifying includes selectively 20 crystallizing the mixture with an organic solvent, or dynamically crystallizing the mixture wherein: selective crystallization comprises combining the mixture and an organic solvent at or above room temperature and warming the combination with stirring to dissolve the mixture and then cooling the combination; and wherein: 25 dynamically recrystallizing the mixture comprises contacting the mixture with a Lewis acid and a solvent optionally including a protic acid; wherein: the organic solvent is selected from toluene, dioxane, THF, or mixtures thereof; the lewis acid is selected from BF 3 -etherates, metal halides, alkoxides, or mixed AUO1 / A(1 1 \.TTD - 3a halide/alkoxides; the mixed halide alkoxides are selected from Al(Oalkyl) 2 C1 or Al(Oalkyl)Cl 2 ); the alkyl group of the alkyl or alkoxide is a straight or branched chain having one to twelve carbon atoms; 5 the metal is aluminum, titanium, zirconium, magnesium, copper, zinc, iron, tin, boron, ytterbium, lanthanum, or samarium; and the protic acid is selected from HCl, HBr, triflic acid, sulfuric acid, phosphoric acid, or combinations thereof. [008] Other aspects of the invention are set forth herein. 10 DESCRIPTION OF THE INVENTION I. DEFINITIONS [009] As used herein, the base used in connection with palladium catalyst and palladium ligand refers to an "inorganic base" or an "organic base". [010] As used herein, "inorganic bases" that may be used in a process of this is invention include, but are not limited to a carbonate salt, a bicarbonate salt, and/or a phosphate salt (and mixtures thereof). In some embodiments of this invention, the inorganic base may be a carbonate salt having the formula MCO 3 , wherein M is an appropriate counter-cation. Examples of carbonate salts include, but are not limited to,

K

2

CO

3 , K 2

PO

4 , Na 2

CO

3 , Li 2

CO

3 , Rb 2

CO

3 , and Cs 2

CO

3 . In some specific embodiments, 20 the inorganic base is K 2

CO

3 or Cs 2

CO

3 . [011] As used herein, "organic bases" that may be used in a process of this invention include tertiary organic bases that include, but are not limited to trialkylamines, e.g. diethylisopropylamine, triethylamine, N-methylmorpholine and the A1Tfl1 /0Ti6l 1 \.TTD like, and heteroaryl amines, e.g. pyridine, quinoline, and the like. [0121 As used herein, "Palladium catalysts" that may be used in a process of this invention include, but are not limited to, Palladium II Salts such as Pd(OAc) 2 and Pd 2 dba 3 . 1013] As used herein, "Palladium ligand" and "Palladium II ligand" refers to a ligand that is capable of forming a complex with the palladium catalyst. Palladium ligands include, but are not limited to, phosphine, bisphosphine, XantPhos, bis(diphenylphosphino)ferrocene and DPEPhos (see Aldrich catalog). See also, WO 95/30680 and US 5,817,848. [0141 "Solvents" for use in this invention include, but are not limited to, toluene, dioxane, and THF, and mixtures thereof. [015] The term "leaving group" refers to a moiety which is replaced by R 3

CONH

2 . Specific groups include, but are not limited to, chloro, bromo, iodo, pseudohalogens, triflate, tosylate, mesylate, and nosylate. [0161 The term "organic moiety" as used in defining variable R 3 refers to any chemical moiety provided that the moiety does not contain a moiety that would interfere with the palladium catalysts. Such interfering moieties would be well known to skilled practitioners and include, e.g., a free sulfhydryl group. A group such as a sulfide or a thiol should not therefore be present in the R 3 organic moiety. Furthermore, the

R

3 organic moiety should not contain an amine group, such as a primary or secondary amine that would be more reactive than the amide of formula (GIIA or GIIB). R 3 may contain primary and secondary amines that are capped with protecting groups that reduce the interaction between the protected amine and the palladium catalysts. [017] As used herein, the term "phase transfer catalyst" means a compound which is capable of transfering a water soluble anion 4 into an organic phase. Phase transfer catalysts include tetralkylammonium salts, phosphonium salts and crown ethers. Examples of phase transfer catalysts include, but are not limited to tetrasubstituted ammonium salts and trisubstituted amines which may form tetrasubstituted ammonium salts in situ. Tetrasubstituted ammonium salts include, but are not limited to, tetrabutylammonium, benzyltrimethylammonium, tetraethylamnonium, cetyltrimethylammonium salts in which the counter ion can be salts bromide, chloride, or iodide. In some examples, the phase transfer catalyst is cetyltrimethylammonium bromide. Trisubstituted amines include, but are not limited to triethylamine, tributylamine,benzyldiethylamine, and diisopropylethylamine. [018] As used herein, the terms "lactone" and "furanone" may be used interchangeably as will be understood by one skilled in the art. [019] As used herein, the term "aliphatic" means straight chained, branched or cyclic Cl-C 12 hydrocarbons which are completely saturated or which contain one or more units of unsaturation. For example, suitable aliphatic groups include substituted or unsubstituted linear, branched or cyclic alkyl, alkenyl, alkynyl groups and hybrids thereof such as. (cycloalkyl)alkyl, (cycloalkenyl)alkyl or (cycloalkyl)alkenyl. [020] The term "alkyl" and "alkoxy" used alone or as part of a larger moiety refers to both straight and branched chains containing one to twelve carbon atoms. The terms "alkenyl" and "alkynyl" used alone or as part of a larger moiety shall include both straight and branched chains containing two to twelve carbon atoms. [0211 As used herein, the term "aryl", used alone or as part of a larger moiety as in "aralkyl", refers to aromatic ring gro-ups having five to fourteen members, such as phenyl, benzyl, 1 5 naphthyl, 2-naphthyl, 1-anthracyl and 2-anthracyl, and heterocyclic aromatic groups or heteroaryl groups such as 2 furanyl, 3-furanyl, N-imidazolyl, 2-imidazolyl, 4-imidazolyl, 5-imidazolyl, 3-isoxazolyl, 4-isoxazolyl, 5-isoxazolyl, a 1,3,4 oxadiazolyl, a 1,2,4-oxadiazolyl, 2-oxadiazolyl, 5-oxadiazolyl, 2-oxazolyl, 4-oxazolyl, 5-oxazolyl, 2-pyrrolyl, 3-pyrrolyl, 2 pyridyl, 3-pyridyl, 4-pyridyl, 2-pyrimidyl, 4-pyrimidyl, 5 pyrimidyl, 3-pyridazinyl, 2-thiadiazolyl, 5-thiadiazolyl, 2-thiazolyl, 4-thiazolyl, 5-thiazolyl, 5-tetrazolyl, 2-triazolyl, 5-triazolyl, 2-thienyl, or 3-thienyl. The term "aryl ring" also refers to rings that are optionally substituted. Aryl groups also include fused polycyclic aromatic ring systems in which a carbocyclic aromatic ring or heteroaryl ring is fused to one or more other rings. Examples include tetrahydronaphthyl, benzimidazolyl, benzothienyl, benzofuranyl, indolyl, quinolinyl, benzothiazolyl, benzooxazolyl, benzimidazolyl, isoquinolinyl, isoindolyl, acridinyl, benzoisoxazolyl, and the like. Also included within the scope of the term "aryl", as it is used herein, is a group in which. one or more carbocyclic aromatic rings and/or heteroaryl rings are fused to a cycloalkyl or non-aromatic heterocyclic ring, for example, indanyl or tetrahydrobenzopyranyl. The term "aromatic ring" or "aromatic group" refers to aryl groups. [022] The term "heterocyclic" refers to saturated and partially unsaturated monocyclic or polycyclic ring systems containing one or more heteroatoms and a ring size of three to eight such as piperidinyl, piperazinyl, tetrahydrofuranyl, pyrrolidinyl, tetrahydropyranyl, morpholinyl, and the like. [0231 As used herein, the term "bicyclic fused ring system" or "bicyclic ring system" refers to two rings which share two atoms. Either ring may be saturated, partially unsaturated, or aromatic. Each ring also may contain 1 to 3 heteroatoms. 6 Examples of bicyclic fused ring systems include, but are not limited to, compounds g, j, k, 1, and m shown in Table 1, and compounds g-l and j-1, 1-1, 1-2, k-1, m-1 and m-2 shown in Table 2. (0241 As used herein, the term "tricyclic fused ring system" or "tricyclic ring system" refers to a bicyclic ring system in which a third ring is fused to the bicyclic ring system such that the third ring shares at least two atoms with the bicyclic ring system. In some embodiments, all three rings share at least one common atom. Any of the rings in the tricyclic ring system may be saturated, partically unsaturated, or aromatic. Each of the rings may include 1 to 3 heteroatoms. Examples of tricyclic ring systems include, but are not limited to, compounds e and q shown in Table 1, and compounds e-1 and q-1 shown in Table 2. [025] As used herein, the phrase "optionally substituted" followed by a chemical moiety (e.g., an optionally substituted aliphatic) means that the chemical moiety may be substituted with one or more (e.g., 1-4) substituents. In some embodiments, aliphatic groups, alkyl groups, aryl groups, heterocyclic groups, carbocyclic groups, and bicyclic or tricyclic ring systems contain one or more substituents. The substituents are selected from those that will be stable under the reaction conditions of the present process, as would be generally known to those skilled in the art. Examples of substituents include halogen, -Qi, -OQi, -OH, protected OH (such as acyloxy), phenyl (Ph), substituted Ph, -OPh, substituted -OPh, -NO2, -CN, -NHQI,

-N(QI)

2 , -NHCOQi, -NHCONHQi, -NQiCONHQi, -NHCON (Qi) 2, -NQiCON (Qi)2, -NQiCOQi, -NHC0 2

Q

1 , -NQiCO 2 Qi, -C0 2

Q

1 , -COQ 1 , -CONHQi, -CON(Qi)2, -S(0) 2

Q

1 , -SONH 2 , -S(O)Qi, -SO 2

NHQ

1 , -SO 2 N(Qi) 2 , -NHS(0) 2

Q

1 , -NQiS(O) 2 Qi, =0, =S, =NNHQi, =NN(Qi) 2 , =N-OQi, =NNHCOQ,, =NNQiCOQ 1 ,

=NNHCO

2 Qi, =NNQlCO 2 Qi, =NNHSO 2 Qi, =NNQiSO 2 Ql, or =NQi where Qi is an 7 aliphatic, aryl or aralkyl group, and each of Q1, the substituted phenyl and the substituted -OPh may be substituted with 1 to 4 of halogen, -Q3, -OQ3, -OH, protected OH (such as acyloxy), phenyl (Ph), -OPh, -NO 2 , -CN, -NHQ 3 , -N(Q 3

)

2 , -NHCOQ 3 ,

-NHCONHQ

3 , -NQ 3

CONHQ

3 , -NHCON (Q3) 2, -NQ 3 CON (Q3) 2, -NQ 3

COQ

3 ,

-NHCO

2

Q

3 , -NQ 3

CO

2

Q

3 , -C0 2

Q

3 , -COQ 3 , -CONHQ 3 , -CON(Q3) 2 , -S(O) 2 Q3,

-SONH

2 , -S(O)Q3, -SO 2

NHQ

3 , -SO 2

N(Q

3

)

2 , -NHS(O) 2

Q

3 , -NQiS(O) 2

Q

3 , =0, =S, =NNHQ 3 , =NN(Q 3

)

2 , =N-OQ 3 , =NNHCOQ 3 , =N-NQ 3

COQ

3 , =NNHCO 2

Q

3 ,

=NNQ

3

CO

2

Q

3 , =NNHSO 2

Q

3 , =NNQ 3

SO

2

Q

3 , or =NQ 3 where Q3 is aliphatic or aryl. [026] As used herein, nitrogen atoms on a heterocyclic ring may be optionally substituted. Suitable substituents on the nitrogen atom include Q2, COQ 2 , S(O) 2

Q

2 , And C0 2

Q

2 , where Q 2 is an aliphatic group or a substituted aliphatzic group. [027] Unless otherwise stated, structures depicted herein are also meant to include all stereochemical forms of the structure; i.e., the R and S configurations for each asymmetric center. Therefore, single stereochemical isomers as well as enantiomeric and diastereomeric mixtures of the present compounds are within the scope of the invention. [0281 The term "substantially pure" refers to the stereochemical purity of a compound that is greater than 90%. In some embodiments, the stereochemical purity of a compound is greater than 95%. And in still others, the stereochemical purity of a compound is 99% or greater. [0291 The term "selective crystallization" means crystallization of a substantially pure isomer from a soJlvent containing a mixture of isomers. [030] The term "dynamic crystallization" rneans crystallization of a substantially pure isomer from a solvent containing a mixture of isomers under conditions which cause isomerization of the mixture of isomers to an isomer which selectively crystallizes. 8 For example, in the case of resolving enantiomers, isomerization of the more soluble enantiomer to the less soluble isomer results in crystallization of the less soluble isomer as the equilibrium between the isomers is driven by crystallization toward the less soluble enantiomer. A specific example of dynamic crystallization may include the epimerization of an anomeric carbon in a solvent under conditions which selectively crystallizes one substantially pure enantiomer. [0311 Unless otherwise stated, structures depicted herein are also meant to include compounds which differ only in the presence of one or more isotopically enriched atoms. For example, compounds having the present structures except for the replacement of a hydrogen by a deuterium or tritium, or the replacement of a carbon by a 1C- or 14 C-enriched carbon are within the scope of this invention. [0321 Various "protecting groups," "capping groups," or "amine capping groups" may be used in the methods of this invention (see, e.g., T.W. Greene & P.G.M. Wutz, "Protective Groups in Organic Synthesis," 3 rd Edition, John Wiley & Sons, Inc. (1999) and the earlier and later editions of this book). Examples of amine capping groups or protecting groups include, but are not limited to, -R 7 , -C(O)R , -C(O)OR', -SOR', -SO 2

R

7 , -SO 3 R,

-SO

2

N(R

7

)

2 , -C(O)C(O)R 7 , -C(O)C(O)OR 7 , -C(O)CH 2

C(O)R

7 , -C(O)N(R 7

)

2 , - (CH 2 ) o- 2

NHC(O)R

7 , -C(=NH)N(R 7 )2, -C(O)N(OR 7 )R', -C(=NOR 7

)R

7 , -P(O) (R 7

)

2 , and -P(O) (OR 7

)

2 ; wherein R 7 is hydrogen, an optionally substituted aliphatic group, an optionally substituted aryl group, or an optionally substituted heterocyclic group. Preferably, R 7 is (Cl-Cl2)-aliphatic-, (C3-C1O)-cycloaliphatic -, (C3-C1O)-cycloaliphatic]-(Cl-Cl2)-aliphatic-, (C6-C1O)-aryl-, (C6-ClO)-aryl-(Cl-C12)aliphatic-, (C3-ClO)-heterocyclyl-, (C6 C10)-heterocyclyl-(Cl-C12)aliphatic-, (C5-ClO)-heteroaryl-, or (C5-C1O)-heteroaryl-(Cl-C12)-aliphatic-. 9 [0331 As used herein, the term "lewis acid" refers to moiety capable of sharing or accepting an electron pair. Examples of lewis acids include, but are not limited to, BF 3 -etherates and metal halides, alkoxides, and mixed halide/alkoxides (e.g., Al(Oalkyl) 2 C1, Al(Oalkyl)C1 2 ). The metals can be aluminum, titanium, zirconium, magnesium, copper, zinc, iron, tin, boron, ytterbium, lanthanum, and samarium. [0341 EDC is 1- (3 -dimethylaminopropyl) -3 -ethylcarbodiimide. HOBt is 1-hydroxybenzotriazole. THF is tetrahydrofuran. TFA is trifluoroacetic acid. DCM is dichloromethane. DMAP is 4 dimethylaminopyridine. DIPEA is diisopropylethylamine. DMF is dimethylformamide. TFA is trifluoroacetic acid. CBZ is benzyloxycarbonyl. 2H NMR is nuclear magnetic resonance. TLC is thin layer chromatography. II. PROCESSES [0351 Processes and compounds described herein are useful for producing caspase inhibitors and/or prodrugs thereof that contain modified aspartic acid derivatives, such as aspartic acid aldehyde moieties. An aspartic acid aldehyde moiety exists in equilibrium with its cyclic hemiacetal form as shown below: 0 O OH W2,'N H W2'N H O H OH where W 2 represents the rest of the caspase inhibitor molecule. Orally available prodrugs of caspase inhibitors have been developed based on the cyclic hemiacetal. For example, the ICE inhibitor 2 including the cyclic hemiacetal is a prodrug being developed as a treatment for rheumatoid arthritis (see US Patent 5,716,929) 10 0 0 N0 N N O :1 NH 00N I H O H O'/ 2. [0361 The general synthetic procedure shown in Scheme 1 is useful for generating a wide array of chemical species which can be used in the manufacture of pharmaceutical compounds. SCHEME 1 O 0 Ra + OR 4 ,__Ra__OR4R

R

3

NH

2 + 4 Palladium Catalyst O X R2 Paladium ligand R NI Base R3 N 2 Phase transfer catalyst H GIII GII Solvent GI The process shown in Scheme 1 includes reacting a compound of formula GII with the amide GIII in the presence of a palladium catalyst, a palladium ligand and a base in a solvent optionally including a phase transfer catalyst and optionally including water to produce the amido carbonyl compound GI. [0371 The moietys X, Ra, R 2 , R 3 and R 4 are defined above. As drawn, GII refers to compounds in which X may be cis or trans to Ra, which provides for both the cis and trans compounds of GI, e.g., R 2 can be cis or trans to Ra. [038] In some embodiments, the process may be used to prepare a compound of formula XIV, when the moietys R 2 and R 4 shown in Scheme I form a substituted heterocyclic ring: Ra O 0 0R H O'R5 XIV 11 wherein R3 and Ra are defined above and R 5 is an optionally substituted aliphatic, optionally substituted aralkyl, optionally substituted heterocyclylalkyl or optionally substituted aryl. Specifically, compound XIV may be produced by reacting a compound of formula XV: Ra O X) O

O'R

5 XV; and a compound of formula XIII: 0

R

3

NH

2 XIII in the presence of a palladium catalyst, a palladium II ligand, a base, a solvent, and optionally a phase transfer catalyst; wherein X, R 3 , and R 5 are defined above. [039] In carrying out the reaction shown in Scheme 1, the reactants and reagents may be used in any molar amount which provides the desired product. In some embodiments, the ratio of the molar amounts of palladium II salt to palladium ligand is between 1:1 to about 1:5. The ratio of the molar amounts of palladium II salt to the reactant GIII can be between about 1:200 to about 1:1, about 1:100 to about 1:25, or about 1:50 to about 1:10. The ratio of the molar amount of the base relative to the GIII is between about 1:2 to about 10:1. The two reactants, GII and GIII,.and the base can be used in nearly equal molar amounts. In some embodiments, the ratio of GII and GIII can be between about 1:3 to about 3:1. [040] The reaction in Scheme I may be conducted at a temperature between 25*C and 120 0 C, e.g., about 50 0 C, in any solvent that does not adversely interfere with the palladium catalyst, the 12 palladium ligand, and the reactants. Examples of suitable solvents are described herein and can include toluene, dioxane, THF, and mixtures thereof. In some embodiments, the solvent may include water. [0411 After obtaining the compound XIV, the compound of formula XVI: O R3 N4 H O'R 5 XVI may be obtained by reducing the furanone ring double bond. [042] The reduction of a furanone ring double bond may be accomplished with a hydride reducing agent, especially a borohydride. Examples of such borohydrides include sodium or lithium borohydride, sodium or lithium triacetoxyborohydride, sodium or lithium cyanoborohydride, tetrabutylammonium cyanoborohydride, sodium or lithium trialkylborohydride, preferably sodium cyanoborohydride. Typically the reaction mixture is adjusted to be mildly acidic, preferably at a pH between 3.0 and 6.0 with acids such as HCl, HBr, acetic acid, formic acid, trifluoroacetic acid, BF 3 .OEt 2 , aluminum trichloride, zinc chloride, or titanium tetrachloride. Optionally, the reaction may be buffered with 1.0-5.0 equivalents of sodium acetate. Optionally, the reaction may be catalyzed by the addition of 1-5% CoCl 2 /semicorrin, ZnCl 2 , or 1-2 equivalents of chlorotrimethylsilane. Chiral hydride reducing agents are known such as R- or S-Alpine Hydride* (lithium B isopinocampheyl-9-bora-bicyclo(3.3.1]nonyl hydride) to provide asymmetric reduction. [0431 Reduction of the ring double bond in, e.g., XIV may also be accomplished by hydrogenation. This is useful when R 5 is stable to the hydrogenation conditions, such as when R 5 is alkyl. 13 Typical hydrogenation conditions include hydrogen gas at a pressure in the range of about one to 100 atmospheres, usually between about 1 to about 20, or about 1 to about 10 atmospheres, and a catalyst present in the range of about 0.01 to 0.5 equivalents per equivalent of XIV (for example). Suitable catalysts include Pd/C, Pd(OH)2, PdO, Pt/C, PtO 2 , preferentially Pt/C or Pd/C. Suitable solvents include ethyl acetate, alcohols, such as methanol, ethanol, isopropanol, aromatic hydrocarbons, such as benzene, toluene, xylene, ethereal such as THF, DME, dioxane, preferentially ethanol or THF. When R 5 is alkyl or aralkyl, such as benzyl, a rhodium (I) or ruthenium (II) catalyst is preferred for stereoselective reduction. Such catalyst is formed by reacting the metal as one of its various complexes with chiral forms of ligands such as methyl- or ethyl DuPHOS (1,1-bis-2,5-dialkylphospholano)benzene, DIOP (2,3-0 isopropylidene-2,3-dihydroxy-1,4-bis(diphenylphosphino)butane), BINAP (2,2'-bis(diphenylphosphino)-1,1'-binaphthyl), CHIRAPHOS bis(diphenylphosphino)butane), BPPM (N-t-butoxycarbonyl-2 (diphenylphosphino)methyl-4-(diphenylphosphino)pyrrolidine), BPPFA (N,N-dimethyl-l-(1',2-bis(diphenylphosphino) ferrocenyl]ethylamine), DEGPHOS (N-benzyl-3,4 bis(diphenylphosphino)pyrrolidine), or alkyl-BPE (bisphospholanoethane). Many other suitable ligands are known in the art. Preferred catalysts are 1,2-bis(2,5-dialkyl phospholano)benzene (cyclooctadiene)rhodium(I) trifluoromethanesulfonate, where alkyl is a straight chain or branched alkyl group of 1-8 carbons, optionally substituted with an aromatic hydrocarbon such as phenyl. [044] Use of the (R,R) isomer of these ligands will lead to the (S)-configuration of the a-amino carbon in the product and use of the (S,S) isomer will lead to the (R)-configuration. Suitable solvents include ethyl acetate, alcohols, such as 14 methanol, ethanol, or isopropanol, aromatic hydrocarbons, such as benzene, toluene, or xylene, ethers such as THF, DME, or dioxane. Preferred solvents are toluene or methanol. The reaction concentration of XIV will typically be in the range of about 0.01M to 1.OM, preferably about 0.1M to 1.0M. The reaction temperature is usually in the range of about 00 C to about 60' C, preferably between about 200 C to about 4 0 * C. (For the use of rhodium catalysts see: G. Zhu, Z. Chen, X. Zhang; J. Org. Chem. (1999) 64, 6907-6910; M.J. Burk, J.G. Allen, W.F. Kiesman; J. Amer. Chem. Soc., (1998), 120, 657-663; M.J. Burk, J.E. Feaster, W.A. Nugent, R.L. Harlow; J. Amer. Chem. Soc., (1993), 115, 10125-10138; For the use of ruthenium catalysts see: J.M. Brown, M. Rose, F.I. Knight, A. Wienand; Recl Trav Chim Pays-Bas, (1995), 114, 242-251; M. Saburi, M. Ohnuki, M. Ogasawara, T. Takahashi, Y. Uchida; Tetrahedron Lett.(1992), 33, 5783-5786; U. Matteoli, V. Beghetto, A. Scrivanti; J Molecular Catalysis A: Chemical 140 (1999) 131 137). (0451 In certain embodiments, when the moiety R 3 includes a chiral carbon bound to the carbonyl of the amide, GIII has the stereochemistry shown in 0 R 3 41-N H2 GIV as for example in the structure GIV' R N *

CONH

2 GIV' 15 The reaction of GIV provides the compound of the formula 0 3 0- N O

OR

5 GV The stereoisomers of GV may be purified by selective crystallization, dynamic crystallzation or chromatography. [046] As described herein, R 3 is any organic moiety. Specifically, it will be understood that the R 3 group may be selected from any organic moiety that is stable to conditions of the coupling reaction shown in Scheme I, such as those conditions described herein. [0471 In specific embodiments, the general process shown in Scheme 1 is useful for producing caspase inhibitors, such as prodrugs of caspase inhibitors, e.g., ICE inhibitors, and intermediates thereof. In these embodiments, R 3 is preferably any moiety that, taken as a whole with the rest of the molecule, provides such an inhibitor. Typically, for caspase inhibitors, the R 3 moiety is specifically referred to in the art as a P2, P3, P4, or combination thereof, moiety or site. Examples of P2, P3,

P

4 moieties are described in more detail below. [0481 The P., moiety terms refer to the amino acid sequence next to the aspartyl cleavage site of a particular caspase substrate. Pi refers to the aspartyl residue of the substrate where caspase induced cleavage occurs in the natural substrate. In the design of new, nonpeptidic caspase inhibitors, the P. designation is often retained to show which portion of the amino acid sequence has been replaced by the non-peptidic moiety. As used herein, the term "P2-P4" moiety refers to either the amino acid sequence described above or a chemical moiety known to replace such a 16 sequence for the purpose of being a caspase substrate, and in particular an ICE substrate. [0491 Examples of P 2

-P

4 moieties that are non-peptidic are described in US 5,919,790 (Allen et al.); US 5,874,424 (Batchelor et al.); US 5,847,135 (Bemis et al.); US 5,843,904 (Bemis et al.); US 5,756,466 (Bemis et al.); US 5,716,929 (Bemis et al.) ; US 5,656,627 (Bemis et al.) ; WO 99/36426 (Warner Lambert); Dolle et al., J. Med. Chem., 40, 1941 (1997); WO 98/10778 (Idun); WO 98/11109 (Idun); WO 98/11129 (Idun) and WO 98/16502 (Warner Lambert), all of which are incorporated by reference. [0501 As would be recognized by skilled practitioners, a P moiety is not necessarily an amino acid residue. For example, a P4 group could be referred to as an amino capping group (e.g., phenyl-C(0)-). Such P4 groups are exemplified herein. [0511 In another embodiment, this invention provides a process for preparing a compound of formula XVI: a R3 N O H O-R 5 XVI; wherein R 3 is a P 4

-P

3

-P

2 moiety of a caspase inhibitor, or portion thereof. Each P 2 , P 3 , and P 4 group may be incorporated into XVI either individually or together. For example, if R 3 is a group other than a P 2 group (e.g., a protecting), the R 3 C=O group may be removed to provide a compound with a free amine group. That amine group and an appropriate P 2 moiety may be coupled under, e.g., standard coupling conditions to provide a compound. wherein R1 is a P 2 moiety of a caspase inhibitor. A P 3 and a P 4 group may be added together or individually in a similar manner. For example, if the P2 moiety is protected, the protecting group may be removed and a P 3 or a P 4

-P

3 - moiety (optionally protected) may 17 be incorporated. If a capping group other than a typical protecting group is desired on any of the terminal P 2 , P 3 , or P 4 residues, such a group may be added routinely by methods known to skilled practitioners. [0521 Accordingly, one embodiment provides a process wherein R 3 is a P 2 - moiety of a caspase inhibitor. [053] Another embodiment provides a process wherein R 3 is a P 3

-P

2 moiety of a caspase inhibitor. [0541 Yet another embodiment provides a process wherein R 3 is a

P

4

-P

3

-P

2 - moiety of a caspase inhibitor. [0551 Another embodiment provides a process wherein R 3 is a P 4

-P

3 P 2 - moiety of a caspase inhibitor, and wherein said moiety is one of the groups listed in Table 1 below; or wherein said moiety is one of the groups listed in Table 2 below. [056] According to another embodiment, R 3 is a P 4

-P

3

-P

2 - moiety wherein the P 4 portion thereof is selected from R-CO, ROC=O, RNHC=O, RC(O)C=O or RSO 2 and R is one of the groups listed in Table 3. [057] According to yet another embodiment, R 3 is a P 4

-P

3

-P

2 - moiety selected from one of the groups listed in Table 4. [0581 In any of the embodiments herein, R 5 is alternatively an optionally substituted group selected from an aliphatic group, aralkyl group, heterocyclylalkyl group and an aryl group. In more specific embodiments, R 5 is methyl, ethyl, propyl, 2-propyl, butyl, pentyl, hexyl, 4-methylpentyl, 2-methylpropyl, cyclopentyl, cyclohexyl, cyclopentylmethyl, cyclohexylmethyl, phenylethyl, phenylpropyl, phenylbutyl, (d)-menthyl, (1) menthyl, 1-adamantyl, 2-adamantyl, 1-indanyl, 2-indanyl, bornyl, 3-tetrahydrofuranyl, benzyl, c-methylbenzyl, 4-chlorobenzyl, 4 fluorobenzyl, 4-methylbenzyl, 4-(2-propyl)benzyl, or 4 trifluoromethylbenzyl. More specifically, R 5 is ethyl or an optionally substituted benzyl; or R 5 is ethyl or benzyl. 18 [0591 In any of the embodiments herein, X is preferably Br. [060] In a specific embodiment, the invention provides a process for preparing a compound of formula I: O YN 00 ox2'4 H 0 comprising: (a) reacting a compound of formula II: 0 Br O II; and a compound of formula III: OyN O

NH

2 III; in the presence of a palladium catalyst, a palladium ligand, a base, optionally a phase transfer catalyst and a solvent to provide the compound of formula I. [061] According to another embodiment, this invention provides a process for preparing a compound of formula IV: 19 0 N0 NN

H

2 N N 0 C1 H O IV; comprising reducing and deprotecting a compound of formula I: H 0 I to provide a compound of formula V: HN 0N \ H 0 V; reacting the compound of formula V with cbz-tert-leucine, under appropriate coupling conditions, to provide a compound of formula VI: 0 0 0J N N0 H 0 VI reacting the compound of formula VI under conditions for removing the cbz group; appropriate conditions would be those that provide an amine (or amine salt) (i.e., under conditions for deprotecting the cbz-protected amine of the tert-leucine, 20 such as, e.g. , H 2 , Pd/C, citrate acid ( (CO 2 H) 2 ) ) ; after deprotection the resultant amine is reacted with 4-amino-3 chlorobenzoic, or a derivative thereof that is suitable for coupling to an amine (e.g., 4-amino-3-chlorobenzoyl chloride), under appropriate coupling conditions, to provide the compound of formula IV. [0621 According to another embodiment, the invention provides a process for preparing a compound of formula IV: 0 N N O H

H

2 N HC' 0 N4 IV; comprising reacting a compound of formula I: ov?'4 H 0 I; under deprotection conditions, that is, under conditions suitable to remove the cbz group of the praline residue, to provide a compound of formula VII: O HN ?4 O N4\ H 0 VII; reacting the compound of formula VII with cbz-tert-leucine, under appropriate coupling conditions, to provide a compound of formula VIII: 21 0 ~~ ON O O O N 0 H 0 0 N4 VIII; reducing and deprotecting the compound of formula VIII to provide a compound of formula IX:

H

2 N ON N H 0 IX; and reacting a compound of formula IX and 4-amino-3-chlorobenzoic acid, or a derivative thereof that is suitable for coupling to an amine (e.g., the 4,6-dimethoxy-2-hydroxypyrazine ester of 4 amino-3-chlorobenzoic acid) , under appropriate coupling conditions, to provide the compound of formula IV. [063] This invention also provides a compound of formula X, wherein the compound is prepared according to the methods herein: RO--N O O N\ H

O-R

5 X wherein: R5 is an optionally substituted group selected from an aliphatic group, aralkyl group, heterocyclylalkyl group or aryl group; and R is H or an amine capping group. 22 [0641 The processes described herein are useful for producing a of formula I: H 0 N4 [065] The process may also be used to produce substantially pure, diastereomers of compound I shown as formulae IA, IB, IC, and ID. 0 0~ ()"O N O I'O NO H 0-- H O IA IB ~0 00N? ()'O N OO 'O N O H H IC ID [0661 Scheme 1 may also produce a mixture of diastereomers IA and IC: O O H 0 IA/C 23 [067] According to another embodiment, this invention provides a process for preparing a compound of formula IA: O N0 O O N H 0 IA comprising the step of selectively crystallizing a compound of formula: j O N O H O IA/C from toluene. [068] This selective crystallization step comprises combining the compound of formula IA/C (i.e., a mixture of IA and IC) and toluene (either at room temperature or above) and warming the combination with stirring to dissolve the compound of formula IA/C and cooling the combination with stirring. Upon cooling, the compound of formula IA is obtained as a crystalline solid (about 96:4 to about 97:3 mixture). [069] According still to another embodiment, this invention provides a process for preparing a compound of formula IA: H 0 IA 24 comprising the step of dynamic crystallization of a compound of formula: 040 O O N H 0 IA/C under in the presence of a Lewis acid and a solvent , optionally including a protic acid. In certain embodiments, the dynamic crystallization is performed with Al(Oalkyl) 3 in toluene. In other embodiments, dynamic crystallization is performed with a lewis acid in a solvent containing a protic acid such as HCl, HBr, triflic acid, sulfuric acid, phosphoric acid, or combinations thereof. [0701 In still other embodiments, the isomers IA and IC may be purified and isolated by known chromatographic methods. [071] In any of the embodiments of this invention involving a compound of formula I, one form of I is represented by the structure: O N 00 H 0 IA. [072] In any of the embodiments of this invention involving a compound of formula II, one form of II is represented by the structure: 25 01 Br g 0 IIA. [073] In any of the embodiments of this invention involving a compound of formula III, one form of III is represented by the structure: O N 0

NH

2 IIIA; [074] In any of the embodiments of this invention involving a compound of formula IV, one form of IV is represented by the structure: 0 0 N N H

H

2 N O N H O IVA. [075] In any of the embodiments of this invention involving a compound of formula V, one form of V is represented by the structure: O HNi Z O N4O H 0 VA. 26 [076] In any of the embodiments of this invention involving a compound of formula VI, one form of VI is represented by the structure: 0 O' O N N 0 HH O 0 i 0 H 0 VIA . [077] In any of the embodiments of this invention involving a compound of formula VII, one form of VII is represented by structure: 0 HN O N Q H 0 VIIA. [078] In any of the embodiments of this invention involving a compound of formula VIII, one form of VIII is represented by structure: 0 ' 0- ' N 0 O O O N O0 H 0 VIIIA. [079] In any of the embodiments of this invention involving a compound of formula IX, one form of IX is represented by structure: 27 N

H

2 N 0 N H 0 IXA. [0801 Also provided are compounds formula XA, XB, XC, or XD, wherein the compound is prepared according to the methods herein: O O O O6 R --N R-N R -- N( RS--N S0 0 Or NN 0 N H O'R 5 H O-R 5 H O'R 5 H O-R 5 XA, XB, XC, or XD wherein: R' is optionally substituted aliphatic, aralkyl, or aryl; and

R

6 is H or an amine capping group. [0811 In one embodiment, R 5 is an optionally substituted group selected from an aliphatic group, aralkyl group, heterocyclylalkyl group and an aryl group. [0821 In another embodiment, R9 is methyl, ethyl, propyl, 2 propyl, butyl, pentyl, hexyl, 4-methylpentyl, 2-methylpropyl, cyclopentyl, cyclohexyl, cyclopentylmethyl, cyclohexylmethyl, phenylethyl, phenylpropyl, phenylbutyl, (d) -menthyl, (1) menthyl, 1-adamantyl, 2-adamantyl, 1-indanyl, 2-indanyl, bornyl, 3-tetrahydrofuranyl, benzyl, a-methylbenzyl, 4-chlorobenzyl, 4 fluorobenzyl, 4-methylbenzyl, 4-(2-propyl)benzyl, or 4 trifluoromethylbenzyl. [0831 In another embodiment, R- is ethyl or an optionally substituted benzyl. [084] In yet another embodiment, R5 is ethyl or benzyl. 28 [085] In one embodiment of this invention, R 6 is an amine capping group and the amine capping group is -C(O)R or -C(O)OR7, and the

R

7 is (C6-C10) -aryl- or (C6 -C10) -aryl- (Cl-C12) aliphatic-, wherein the aryl is optionally substituted. In one form of this embodiment, -C(O)OR , wherein R 7 is optionally substituted benzyl, preferably benzyl. [0861 Any amines obtained as described herein, may be used with or without isolation from the reaction mixture. The desired caspase inhibitor prodrug rnay be derived from, e.g., V, VII, or the free amine of XIV (either as depicted or in the reduced form) by attaching the appropriate P 2 , P 2

-P

3 , or P 2 -P3-P 4 moiety. A coupling of an amine with such a moiety may be carriecL out using the corresponding carboxylic acid, or reactive equivalent thereof, under standard amide bond-forming or coupling conditions. A typical coupling reaction includes a suit-able solvent, the amine in a concentration ranging from about. 0.01 to 10M, preferably about 0.1 to 1.OM, the requisite carboxylic acid, a base and a peptide coupling reagent. [087] If an amine is used without isolation, the coupling may be carried out in situ in the solvent of the reaction mixture used in the preparation of the amine, or in a different solvent. To this reaction mixture, the requisite carboxylic acid may be added and the reaction maintained at a temperature in the range of about 00 to 1000 C, preferably between about 200 to about 400 C. The base and peptide coupling reagent are then added. to the mixture, which is maintained at a temperature in the range of about 00 to about 60*C, preferably between about 20* to about 40 0 C. The base is typicall-y a tertiary amine base, such as triethylamine, diisopropylethylamine, N-methylmorpholine, DBU, DBN, N-methylimidazole, preferably triethylamine or diisopropylethylamine. The amount of base used is generally up to about 20 equivalents per equivalent of the amine (e.g., IV), 29 preferably at least about 3 equivalents of base. Examples of peptide coupling reagents include DCC (dicyclohexylcarbodiimide), DIC (diisopropylcarbodiimide), di-p toluoylcarbodiimide, BDP (1-benzotriazole diethylphosphate- 1 cyclohexyl-3-(2-morpholinylethyl)carbodiimide), EDC (1-(3 dimethylaminopropyl-3-ethyl-carbodiimide hydrochloride), cyanuric fluoride, cyanuric chloride, TFFH (tetramethyl fluoroformamidinium hexafluorophosphosphate), DPPA (diphenylphosphorazidate), BOP (benzotriazol-l yloxytris (dimethylamino) phosphonium hexafluorophosphate), HB3TU (0-benzotriazol-1-yl-N,N,N',N'-tetramethyluronium hexafluorophosphate), TBTU (O-benzotriazol-1-yl-N,N,N',N' tetramethyluronium tetrafluoroborate ), TSTU (0-(N succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate), HATU (N-[(dimethylamino)-l-H-1,2,3-triazolo[4,5,6]-pyridin-1 ylmethylene] -N-methylmethanaminium hexafluorophosphate N-oxide), BOP-Cl (bis(2-oxo-3-oxazolidinyl)phosphinic chloride), PyBOP ((l-H-1,2,3-benzotriazol-1-yloxy)-tris(pyrrolidino)phosphonium tetrafluorophopsphate), BrOP (bromotris (dimethylamino) phosphonium hexafluorophosphate), DEPBT (3- (diethoxyphosphoryloxy)-1,2,3-benzotriazin-4(3H) -one) PyBrOP (bromotris (pyrrolidino) phosphonium hexafluorophosphate). EDC, HOAT, BOP-Cl and PyBrOP are preferred peptide coupling reagents. The amount of peptide coupling reagent is in the range of about 1.0 to about 10.0 equivalents. Optional reagents that may be used in the amide bond-forming reaction include DMAP (4 dimethylaminopyridine) or active ester reagents, such as HOBT (1-hydroxybenzotriazole), HOAT (hydroxyazabenzotriazole), HOSu (hydroxysuccinimide), HONB (endo-N-hydroxy-5-norbornene-2,3 dicarboxamide), in amounts ranging from about 1.0 to about 10.0 equivalents. 30 [0881 Alternatively, one may treat an amine with a reactive equivalent of the R 3 COOH carboxylic acid, such as P 2 -, P 3

-P

2 -, or

P

4

-P

3

-P

2 -C(=O)Xl, where C(=O)X 1 is a group that is more reactive than COOH in the coupling reaction. Examples of -C(=O)X' groups include groups where X1 is Cl, F, OC(=O)R (R = aliphatic or aryl), SH, SR, SAr, or SeAr. [0891 A number of chemical groups are known that may be used as the P 3

-P

2 - portion of the ICE or caspase inhibitor prodrug. Examples of such P 3

-P

2 - groups are shown in Table 1 as part of a

P

4

-P

3

-P

2 - moiety. 0 P4-P3-P2 N ORS H OR 5 Table 1. P 4

-P

3

-P

2 - Groups R6 X

AA

3 H AA 3

R

6 NAA 7CH 2 )n P4, - Nr P4- P4, N-C2) N ~ ~ N jy H ~ 2H H O MA2 O O a b c

A

2 A 2 N (CH 2 )n X N N P 4 'N N AA 2 P4'N HOP4'N H O H O d e f 31 R5 R 5

A

2 P44 N N A P4 N H N H 0 g h ' 2 (CH 2 )n NI N N O N N HH0 H O k (cH 2 )n R P4 HNP4'NH N P4N 0 ~~ O AA2 m n 0 R5 R R

R

5

R

6 R 6 AA 3

(-

I N N~ , R 4 "'N H R4 H O O p q r where n is zero to three; AA refers to an amino acid side chain; X is N, 0, S, SO, SO 2 , CHF, CF 2 , C(R 3

)

2 , C=0, or C=NOR; A 2 is 0, S or H 2 ; Y is N or CH; R is hydrogen, Cl- 1 2 alkyl group, aryl group, or heteroaryl group, the R groups being optionally substituted with one or more halogen; R 3 is an alkyl having one to six carbons; R 4 is R-CO, ROC=O, RNHC=O, RC(0)C=0, or RS0 2 ; and R 5 is hydrogen, halo, alkyl, alkoxy, haloalkyl, haloalkoxy, amino, phenyl, phenoxy, hydroxy, alkoxycarbonyl, carboxyl, alkylcarbonyl, alkylsulfonyl, alkylsulfoxyl, alkylcarbonylamino, alkylcarbonylalkylamino, alkylamino, dialkylamino, aminosulfonyl, or cyano; and R 6 and R7 are independently selected 32 from R 3 , aryl, heteroaryl, (C 1

-

1 2 alkyl) aryl,

(C

1

..

1 2 )benzocycloalkyl, or (C 1

-

1 2 alkyl)heteroaryl. [090] Preferred P 4

-P

3

-P

2 - groups are shown in Table 2. Table 2. Preferred P 4

-P

3

-P

2 -- Groups P4'N P4N 6P4 N O HKO P4,N N ~~N H H M N H 0 a-l b-1 c-i F P4, N P4 ?N P4, N 0 H 0 0 c-2 c-3 c-4 NF 0 O O N P 4 N§IOY c-5 c-6 d-1

CH

3 P4, ON P N N? N 0 H 3 H 0 d-2 e-1 f-1 HO N N CH3 N 4 N

CH

3 PN H 0 P- 0 H O CH 3 H g-1 h-1 i-i 33 -0 N O P N N 'N N P-- ( H0 PN H- H H 0 j-1 k-1 1-1 P44N P1NN N Q N P4, N N S H P4-N 0 1-2 m-1 m-2 P4N N NF 0 N P4'N N H O H 0 o-1 o-2 o-3 --

SO

2

CH

3 I N

P

4 O N

P

4 -C 6H 3 H 0r H 0 p-1 q-1 r-1

H

2 N 0 P41N N -- 04No H r-2 n-i where R 6 is an optionally substituted benzyl as des cribed below or 2-indanyl, and the P4 moiety is represented by R-T-, wherein R-T- is R-CO, ROC=O, RNHC=0, RC(O)C=0, or RSO 2 {0911 Preferred R groups of P 4 are shown in Table 3. 34 0

R-T-P

3 -P2 N4O H OR2 Table 3. Preferred R Groups of P 4

CH

3 0 &C CI, 100 101 102 103 104 C I HCO H 2 N H3C O Cl CI Ci Cl Cl 105 106 107 108 109 F H 3 C N HO H3CO 110 111 112 113 114 N H 3 Cq N H3C N N

H

2 N HO 0 H 2 N C F CH 3

CH

3 cl 115 116 117 119 120

H

2 N 0O HN NHN H3C"N H O H 02 121 122 123 124 0

XCH

3

H

3 C N 02 OH cl 125 126 127 128 35 F OMe CI CI 'N ..N -NK OMe OMe CI 129 130 131 132 133 '~0

H

2 N OH H 134 135 136 137 138 q~ Q-VN N 1 -- 139 140 141 142 143

C-

12 Alkyl N N N 0N O 144 145 146 147 148 N N N> 0"- C) H 149 150 151 152 153 NI N -' N _ 154 155 156 157

H

3 C OJN N CI 158 159 160 [092] In specific embodiments, R-T- is R-CO where R is 1 naphthyl, 2-naphthyl, 1-isoquinolinyl, or 36 3 N 4 5 where positions 3 and 5 of R are independently and optionally substituted by halogen, preferably chloro, or C 1 .3 alkyl, and position 4 is optionally substituted by amino, acetamido, hydroxy or methoxy. [093] The most preferred P 4

-P

3

-P

2 - groups are shown in Table 4. Table 4. Most Preferred P 4

-P

3

-P

2 - Groups Rs 0 HP R HN RRN 0H a-la b-1a b-lb o R 4 010 0 R IHN N? R HN N R HN RHN 0 0 0 d-la g-1a g-2a Rk N R'HN O N R N R NK o 0 I0 o-2a c-7a c-la R I N c-6a where R is, referring to Table 3, one of the following groups: 100, 105, 107, 108, 114, 117, 119, 126, 136, 139, 140, and 141. 37 [094] In attaching the P 4

-P

3

-P

2 - moiety, or portion thereof, the moiety may be attached in one piece as or subunits of the moiety may be added in a sequential manner as described above. For example, Cbz-protected proline may be coupled to XV (or if R 5 is ethyl with II): 0 N |0 Z NJ O H O'R5 [095] After removal of the Cbz group, a P 3 or P 3

-P

4 moiety may be attached by alkylation or acylation of the proline nitrogen. [0961 In certain embodiments, methods of the present process proceed through the butenolactone XV where X is chloro, bromo or iodo: O I O0 xs O'RG XV. A preferred starting butenolactone is the bromofuranone XV (wherein X = Br), which may be obtained according to Escobar et al., An. Quim., 1971, 67, 43. Alternatively, other reactants of the formula GIIA and GIIB may be commercially available or produced from know methods. See, for example, "Comprehensive Organic Transformations: A Guide to Functional Group Preparations," 2nd Edition, by Richard C. Larock, pages 638, 659, 661, 724. [097] Also within the scope of this invention, another embodiment of the coupling reaction of an amine proceeds by acylation of the anion of the amine using a reactive equivalent of the carboxylic acid, such as P 2 -, P 2 -P3-, or P 2

-P

3

-P

4 -C(=O)X, where C(=O)X is as described above. The anion of the amine is first generated by treating the amine in a solvent with any suitable 38 base. Examples of solvents that may be used include ethereal solvents such as THF, DME, dioxane, diethyl ether, methyl-tert butyl ether; aromatic hydrocarbons, such as benzene, toluene, xylene; halogenated hydrocarbons, such as dichloromethane, carbon tetrachloride, dichloroethane; or other organic solvents, such as acetonitrile. Preferred solvents include THF, DME, toluene or dichloromethane. Suitable bases for generating the anion include organic bases such as an alkali metal hydride, an alkali metal tert-butoxide, an alkyl or aryl lithium, such as methyl-, butyl- or phenyllithium; an alkali metal amide, such as lithium-, sodium- or potassium bis(trimethylsilyl)amide, diisopropylamide, or tetramethylpiperidine. Preferred bases include lithium bis(trimethylsilyl)amide, lithium diisopropylamide, or lithium tetramethylpiperidine. The anion of the amine is treated with the carboxylic acid equivalent at a reaction temperature that may be in the range of about -78*C to 120 0 C, preferably between about 0*C to 60 0 C. [0981 Reduction conditions for reducing the double bond in the furanone ring may also be used as deprotection conditions. For example, when R 3 (in XIV) or R 6 (in X) is cbz, conditions may be used to reduce the double bond and to also remove the cbz group. [099] Methods herein describe a sequence in which the butenolactone is first coupled to a caspase P, or Px.y moiety and then the ring double bond is reduced. Alternatively, the reduction and coupling may be performed in reverse order. [0100] In still another embodiment, this invention provides a process for preparing a compound of formula XVI: 0 RN< H O'R 5 XVI; 39 wherein R 3 is a P 4

-P

3

-P

2 moiety of a caspase inhibitor, the P 4

-P

3 P 2 is c-1 of Table 2, the P 4 is 108 of Table 3, R 5 is as defined herein (e.g., ethyl) and the process is according to the methods herein. [01011 This invention also provides a process for preparing a compound of formula IVA: 0 O N N H H2N /0

N

C1 H 0 IVA; comprising selective crystallization of a compound of formula: 0 O N O OON4 H O IA/C from toluene. [01021 Alternatively, a process for preparing a compound of formula IVA: 0 O N N

H

2 H H 0 CI IVA; comprises dynamic crystallization of a compound of formula: 40 C l" O N O H 0 IA/C by contacting the mixture of IA/C with a Lewis acid in a solvent optionally including a protic acid. [0103] This invention also provides a process for preparing a compound of formula IVA: 0 N

H

2 N 0 N H O C1 IVA; comprising, reacting a compound of formula II: a Br'4O II; and a compound of formula III: ()"O YN K 0V? 0

NH

2 III; in the presence of a palladium catalyst, a palladium ligand, and a base in a solvent optionally including a phase transfer catalyst. 41 [0104] Also provided are methods of preparing the corresponding aldehyde compound (of e.g., XVI) by these processes. For example, compound IV prepared according to this invention, may be converted to the corresponding aldehyde compound, that is by converting the furanone to an aldehyde. [0105] In another embodiment, this invention provides a process for preparing a compound of formula XVI: 0 R3 N H O-R 5 XVI; wherein R3 is a P 4

-P

3

-P

2 moiety of a caspase inhibitor, the P 4

-P

3 P 2 is d-1 of Table 2, P 4 is 141 of Table 3, Rs is as defined herein (e.g., ethyl), and the process is according to the methods herein. [0106]Accordingly, this compound (see compound 412f and/or corresponding compound 412 as disclosed in WO 97/22619, which is incorporated herein by reference) is prepared by reacting a compound of formula II: O Br g ON II; and an appropriate amide compound, in the presence of a palladium catalyst, a palladium ligand, a base, optionally a phase transfer catalyst and an appropriate solvent. An appropriate amide compound would be derived from the P4-P3-P2 group d-la in Table 4, i.e., a compound: 42 0 N RN H OO NH2. wherein R is either H or an isoquinolinoyl (i.e., the P4 group 141 in Table 3, wherein there is a carbonyl linker between the compound and the isoquinolinoyl group. [0107] In still further embodiments, the invention provides a process of preparing a beta-amido carbonyl compound of formula XXX: R 0 R OR 2

R

3 N R H XXX comprising the steps of : a) reacting a compound of formula XII: Ra O 1OR2 X R4 XII; with a compound of formula XIII: O R

NH

2 XIII; in the presence of a palladium catalyst, a palladium ligand, a base, in a solvent optionally a phase transfer catalyst, to produce a compound of the formula XXXI 43 0 Ra O OR 2

R

3 N R4 H XXXI wherein: X is a leaving group; Each Ra is H, an optionally substituted alkyl, an optionally substituted aryl, -CN, -C(O)-Oalkyl or halogen; Each R 2 is independently an optionally substituted aliphatic group, an optionally substituted heterocyclic group, and an optionally substituted aryl group; Each R 4 is independently an optionally substituted aliphatic, an optionally substituted heterocycle, an optionally 24 substituted aryl, or R and R 4 together with the groups to which they are bound, form an optionally substituted 5- to 8-membered heterocyclic ring; Each R 3 is an optionally substituted aliphatic, an optionally substituted aryl, an optionally substituted heteroalkyl, a protecting group, P 2 -, P 3

-P

2 -, or P 4

-P

3

-P

2 -;

P

2 - is

R

5 R8-N/

P

3

-P

2 is Ry

R

6

R

8 -N 44

P

4

-P

3

-P

2 - is

R

7 R6 ;

P

4 -N H P4 is R- T; T is -C(0) -, -0-C(O)-, -NHC(0) -, -C(0)C(0) - or -SO2-; Each R is independently an optionally substituted aliphatic, an optionally substituted aryl, or P 2 ; Each R 5 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl; Each R 6 is independently an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, an optionally substituted phenyl, or R 5 and R 6 taken together with the atoms to which they are attached form a 5 to 7 membered, optionally substituted monocyclic heterocycle, or a 6 to 12 membered, optionally substituted bicyclic heterocycle, in which each heterocycle ring optionally contains an additional heteroatom selected from -0-, -S- or NR5o-; Each R 7 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl, or

R

7 and R 6 together with the atoms to which they are attached form a 5 to 7 membered, optionally substituted monocyclic heterocycle or aryl (see, for example, compounds f, h, i, n, and o shown in Table 1 and compounds o-1, o-2, and o-3 shown in Table 2), or a 6 to 12 membered, optionally substituted bicyclic fused ring system, in which 45 each of the fused rings optionally contains an additional heteroatom selected from -0-, -S- or -NR 5 0 - (see, for example, compounds g and j shown in Table 1, and compounds g-1 and j-1 shown in Table 2), or when R 5 and R 6 together to with the atoms to which they are attached form a ring, R 7 and the ring system formed by

R

5 and R 6 form a 8- to 14- membered optionally substituted bicyclic fused ring system (see, for example, compounds g, k, 1, and m, shown in Table 1 and compounds d-1, d-2, k-1, 1-1, 1-2, m-1, and m-2 shown in Table 2), wherein the bicyclic fused ring system is optionally further fused with an optionally substituted phenyl to form an optionally substituted 10- to 16-membered tricyclic fused ring system (see, for example, compounds e and q shown in Table 1, and compounds e-1 and q-1 shown in Table 2); Each R 8 is independently H or a protecting group; and Each R 50 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl; and m is 0 to 2. [0108] In some embodiments, R 3 is an organic moiety. [0109] In certain embodiments, the variable R in P 4 may be an aliphatic, aryl, or heteroaryl, each optionally substituted with 1 to 3 aliphatic, halo, alkoxy, -CN, -NO 2 , -N(R 5 0

)

2 , -SOmN(Rso) 2, -NC(O) R 50 , -SOmRso or heterocycloalkyl. [0110] The process further comprises reducing the compound of formula XXXI to produce a compound of Formula XXX. [0111] In some embodiments P 2 - has the structure A 46 in which Ring A is a 5 to 7 membered, optionally substituted monocyclic heterocycle, or a 6 to 12 membered, optionally substituted bicyclic heterocycle, in which each heterocycle ring optionally contains an additional heteroatom selected from -0-, -S- or -NR 5 0 -, R 50 is H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl. [0112] In specific embodiments, Ring A has the structure: F-11 N NN N N N [0113] P 2 - has the structure (AD [01141 In certain embodiments, Ring A has the structure VN? VN 7 N N N [01151 In specific embodiments P 2 - has the structure Ra....-N Ra _-N or [0116] In another embodiment, the a process for producing a compound of the formula 47 O 0

R

5 Ra H OR2 R-T N N R 4 H

R

7 R6 O comprises: a) reacting a compound of the formula: 0I Ra OR2 4R4 with a compound of the formula: 0

P

2 NH 2 in the presence of a palladium catalyst, a palladium ligand, a base, optionally a phase transfer catalyst and a solvent, to produce a compound of the formula 0 0 OR 2 P2 N H R wherein: X is a leaving group such as Br; Each Ra is H, an optionally substituted alkyl, an optionally substituted aryl, -CN, -C(0)-Oalkyl or halogen; Each R 2 is independently an optionally substituted aliphatic group, an optionally substituted heterocyclic group, and an optionally substituted aryl group; 48 Each R 4 is independently an optionally substituted aliphatic, an optionally substituted heterocycle, an optionally substituted aryl, or R2 and R 1 together with the groups to which they are bound, form an optionally substituted 5- to 8-membered heterocyclic ring;

P

2 is

R

5

R

6 T is -C(O)-, -0-C(O)-, -NHC(O)-, -C(O)C(O)- or -SO2- ; Each R is independently an aliphatic, or aryl, each optionally substituted with 1 to 3 aliphatic, halo, alkoxy, -CN,

-NO

2 , -N(Rso) 2 , -SOmN(Rso) 2 , -NC(O)R 5 0 , -SOmRso or heterocycloalkyl; Each R5 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl; Each R 6 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, an optionally substituted phenyl, or R 5 and R 6 taken together with the atoms to which they are attached form a 5 to 7 membered, optionally substituted monocyclic heterocycle, or a 6 to 12 membered, optionally substituted bicyclic heterocycle, in which each heterocycle ring optionally contains an additional heteroatom selected from -0-, -S- or

-NR

7 -; Each R7 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl; Ra is H or a protecting group; and 49 m is 0 to 2. [01171 In a specific embodiment, a compound of the formula 0 X 0

O-R

9 in which R 9 is Ci-C 5 alkyl, is reacted with O

P

2

NH

2 to produce the compound

P

2 N H

O-R

9 [0118] The process may further comprise the step of contacting a racemic mixture of compounds of the formula 0

P

2 N H O-R 9 in which R 8 is a protecting group, with a Lewis acid, optionally in the. presence of a protic acid, in an organic solvent to provide compounds having the structure O N 4 P2 ) NJ H O-R 9 [0119] In some embodiments, resolution of the alkoxyfuranones may be achieved by selectively recrystallization from an organic solvent. 50 [0120] The process may include reducing the double bond in XXXI. For instance, the compound of the formula 0 O Ra Ra O Ra OR 2 O OR2

R

3 N R4 P 2 N R 4 H H or is reduced under conditions described herein to provide a compound of the formula O 0 Ra OR2 P2 N R 4 H [0121] After producing this compound, P 3 - and P 4 - moieties may be coupled to the compound as described above. [01221 In still another embodiment, the process for producing a compound of the formula oO R-T Ra

OR

2 R-T N N R 4 1 0 H

R

7 R 0 comprises: (a) reducing the compound of the formula 0 Ra 0 OR2

P

2 N R 4 H to provide a compound of the formula 51 0 Ra O2OR2

P

2 N R 4 H wherein Each Ra is H, an optionally substituted alkyl, an optionally substituted aryl, -CN, -C(O)-Oalkyl or halogen; Each R 2 is independently an optionally substituted aliphatic group, an optionally substituted heterocyclic group, and an optionally substituted aryl group; Each R 4 is independently an optionally substituted aliphatic, an optionally substituted heterocycle, an optionally substituted aryl, or R 2 and R' together with the groups to which they are bound, form an optionally substituted 5- to 8-membered heterocyclic ring;

P

2 is

R

5 R-N /

R

6 T is -C(O)-, -O-C(O)-, -NHC(O)-, -C(O)C(O)- or -SO2-; Each R is independently an aliphatic, or aryl, each optionally substituted with 1 to 3 aliphatic, halo, alkoxy, -CN, -N0 2 , -N(R 5 0

)

2 , -SOmN(Rso) 2 , -NC(O)R 5 0 , -SOmR 5 o or heterocyclcalkyl; Each R 5 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl; Each R 6 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, an optionally substituted phenyl, or R 5 and R 6 taken together with the atoms to which they are attached 52 form a 5 to 7 membered, optionally substituted monocyclic heterocycle, or a 6 to 12 membered, optionally substituted bicyclic heterocycle, in which each heterocycle ring optionally contains an additional heteroatom selected from -0-, -S- or -NRso- ; Each R 50 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl; Re is a protecting group; and m is 0 to 2. [0123] A process for producing a compound of the formula R10---N A H O N | 0 o O-R 9 comprises: a) contacting a racemic mixture of compounds represented by the formula R1O-N A 0 N Oe' 00-R 9 with a Lewis acid in an organic solvent optionally including a protic acid, wherein Ring A is a 5 to 7 membered, optionally substituted monocyclic heterocycle, or a 6 to 12 membered, optionally substituted bicyclic heterocycle, in which each heterocycle ring optionally contains an additional heteroatom selected from -0-, -S- or -NR 5 o-; Each R 9 is a Ci-Cs alkyl; 53 Each R10 is H, a protecting group, P 3 - or P 4

-P

3 -; P3 is

R

7

R

8 -N _I H

P

4 is R-T; T is -C (O) -, -O-C (O) -, -NHC (O)-, -C (O) C (O) - or -S0 2 -; and Each R is independently an aliphatic, aryl, or a heteroaryl, each optionally substituted with 1 to 3 aliphatic, halo, alkoxy, -N(R 5 0

)

2 , -SOmN(R 50

)

2 , -NC(O)R 5 0 , -SOmRso or heterocycloalkyl; Each R 7 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl, or

R

7 and the Ring A form a 8- to 14- membered optionally substituted bicyclic fused ring system, wherein the bicyclic fused ring system is optionally further fused with an optionally substituted phenyl to form an optionally substituted 10- to 16-membered tricyclic fused ring system; Each R 50 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl; R6 is a protecting group; and m is 0 to 2. [0124] Alternatively, resolution of

R

1 0 -N A 0 OY 0 O-R9 54 may be achieved by chromatography or selective crystallization from an organic solvent. [0125] A process for producing a compound of the formula R1I--N A H O N O 0

O-R

9 comprises: a) reducing the compound of the formula R10---NA H 0. o O-Rq wherein Each R1 0 is H, a protecting group, P 3 - or P4-P3-;

P

3 - is R7

R

8 -N H 0

P

4

-P

3 - is

R

7

P

4 -N H 0

P

4 is R-T-; T is -C(O)-, -0-C(O)-, -NHC(0)-, -C(O)C(O) - or

-SO

2 -; 55 Each R is independently an aliphatic, aryl, or heteroaryl, each optionally substituted with 1 to 3 aliphatic, halo, alkoxy, -CN, -NO 2 , -N(R 50

)

2 , -SOmN(R 5 0

)

2 , -NC(O)R 5 o, -SOmRso or heterocycloalkyl; Each R 7 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl, or

R

7 and the Ring A form a 8- to 14- membered optionally substituted bicyclic fused ring system, wherein the bicyclic fused ring system is optionally further fused with an optionally substituted phenyl to form an optionally substituted 10- to 16-membered tricyclic fused ring system; Each R 50 is independently H, an optionally substituted aliphatic, an optionally substituted heteroalkyl, an optionally substituted heteroaryl, or an optionally substituted phenyl; RB is a protecting group; and m is 0 to 2. The processes described herein can be used to produce aspartic acid derivatives such as aspartic acid aldehyde moieties. For instance, the processes described herein can be used to produce compounds containing the P4-P3-P2- moieties shown in Tables 1, 2, 3, and 4 shown above and the specific compounds in Tables 5 and 6 below. Additionally, the processes described herein may be used to produce known compounds. Specifically, the processes are useful for preparing the compounds disclosed in WO 95/35308, WO 99/47545, WO 04/058718, WO 04/002961, WO 04/106304, WO 03/088917, WO 03/068242, WO 03/042169, WO 98/16505, WO 93/09135, WO 00/55114, WO 00/55127, WO 00/61542, WO 01/05772, WO 01/10383, WO 01/16093, WO 01/42216, WO 01/72707, WO 01/90070, WO 01/94351, WO 02/094263, WO 01/81331, WO 02/42278, WO 03/106460, WO 03/103677, WO 03/104231, US 6,184,210, US 6,184,244, US 6,187,771, US 6,197,750, US 6,242,422, US 6,235,899, April 2001 56 American Chemical Society (ACS) meeting in San Diego, California, USA, WO 02/22611, US2002/0058630, WO 02/085899, WO 95/35308, US 5,716,929, WO 97/22619, US 6,204,261, WO 99/47545, WO 01/90063, Bioorg. Med. Chem. Lett. 1992, 2(6), 613, and WO 99/03852, all of which, as set forth herein, are incorporated herein by reference. Preferred compounds for use in accordance with this invention are described in WO 04/058718, WO 04/002961, WO 95/35308, US 5,716,929, WO ''~'/-22619, US 6,204,261, WO 99/47545, and WO 01/90063, all of which, as set forth herein, are incorporated herein by reference. Table 5 F 0 H O H O H I-1 1-2 1-3 H O H O H 0 I-7 I-8 I-9 CN N N OO N H 0 H 0 H 0 I-10 I-11 I-12 57 0 H0 0 0 qo0 H 0H 1-1 1-1 1-1 5 CFl C30 WHN2 QJN1< 0 *J-JO O A 0 ON 0 N0 H 0 ~ H 0 H 0 1-1 1-17 1-1 CF30 Cl0 O NI12N 5 N N < & ZN 7N H~o H N-O H N o FTN - I H~j 0 H /HO 1 19 2 1120126 1-22 1-231-2 FC -O Cl 00 cl UO58 WH ~ 0 W 0-2 A-3 1-3 H H 1-29 1130133 H 011 0 0 N o HOiCI 0A H 0H ~ 1--32 1-331-3 HNAO~7 HN 5 N 0 H) N& 7 0TNf H 0H 0 H 1-4 1- 4 1-4 cl 0 cl 0 H C59 Hl-0c 0 NC. O N HO OW N O N O H4 (0 H H ~0 H 0 H OH O H 0 I-46 I-47 I-48 H N I-49 I-50 I-51 ON 0 0 N < H O H O I-549 -55 I-56 2CI O O CCl 0O O H0 H O 0 O~~ O OH yO 0100 00 H O H O 1-57 1-58 60 4-7, 1-59 1-60 N0 I-61 I-62 ~ H CI 0 F l 0 N NO N I-63 I-64 O k N O 1-65 1-66 0 H 0 0 1-67 1-68 1-69 1-70 61 H 0IN4 H2N 0 0 N

H

2 N PAHC P 1-71 17 o 0 N~H 2 0 N 0 1-73 Table 6: 0l H 0 H 0 H 0 Il-i11-2 11-3 F F 0 0 0N N a HOy)N & 'Zd OH H OHH Oj( H 00NH 00N H

H

0 H 00 H o 0 H 0 11-4 Il-5 11-9 0 0 T H 0 H 0 H 0 11-7 1 -8 162 cI

ON

4 OH &!11 N0H 7 O 0 0 0 11-13 11-14 -1 )N~0& N40H CIJ7N 0 0NT N H0 H0 H0 H H 0 H 0 HH CIl , N) N H -H'i OH H O 0 OY NMeO' A 0l 0 H H 0 0 -0 TN MeO N

H

0 HH 0 11-19 11-23 11-21 0 0H 0 H 0 H CF0Q MeOTN Me T o H 0

H

0 H 0 11-22 11-2 11 0

H

0 11-25 11-2612 cl 0 CF3 QJH OH OH 0 H0 H H 0 11-33 11-34 11-35 t HOH 0 &Y $OH &HN NT OH N 0 N"'f H 0 ON 11-36 11-37 11-38 NCrN 0 HN 0 1 OAH OH HN H %H ~ N~ H 0 H N ' 0 Hi0 H O N.H 04\ 0 0 .0

H

0 0 0 11-39 11-40 11-41 0< ci 0 HN 100 o F 0 OAN NNK f"N IN OH N-.-- H0 2OH IHN-C N Ny \ 0N- H 0 N f H HN, Hn 0 O-rH H 0 H 0 H 0 11-42 11-43 11-44 F o 0l 0 ) r 9 0.N 0 fH 0 HN>N0 H Ol 0 N( N OH H0 NN~( O H 0 H 0 H 0 11-45 11-46 11-47 oI CI Cl 0 0 0 H H#-"-NTN HNH T'( H 0

H

0 0 11I-48 11-49 11-50 9 0 a ~ O 0 C) 0 N 0 OWH o Nw N0ON a H 0 H 0 H 0 11-51 11-52 11-53 cl0 0 CH 3 O0 0 NONYlo9 4 N H 00 11-54 11-55 64 N OH N OH -71 0 IN. 00 0 11-56 11-57 II-6 0II1 0 0N NO4OH N N OH 1-62 II-63 (J i ~ O4 H H2N rN 4 OH N0 H 0 H 11-64 11-65

H

2 N N OH 11-66 [0126] In order that this invention be more fully understood, the following preparative examples are set forth. These examples are for the purpose of illustration only and are not to be construed as limiting the scope of the invention in any way. 65 Examples [01271 The abbreviations used herein are known to skilled practitioners. Scheme 1 the syntheses that are exemplified below. Scheme 2. Synthetic Examples STEP 10 C NH2 O N 2 ZN O z =% ZN 0, 2% Pd(OAc) 2 , Xanth N Br O0 PhCH 3 Cs 2

CO

3 H OEt 22 g Ex. 1 STEP 2 z 4 a) PhOH 3 N O O N N H OEt b) AI(OEt) 2 HCI H OEt Ex. 1 PhCH 3 Ex. 2 STEP 3 Z P d / C , H 2 HO H H H ORt H HNXJ H0. OH L ORI OEt Ex. 2 Ex. 3 Example 1 KO (D O "0 N O H 0 1 Preparation of 4-bromo-5-ethoxy-5H--furan-2-one [0128] This procedure may be carried out in a manner similar to that described by C. Escobar, et -al., Ann. Quim. (1971), 67, 43 57.). To a solution of 5-ethoxy-5H-furan-2-one (II, R 1 =Et) (10.0 g, 78.0 mmol) in carbon tetrachloride (50 mL) at 0 0 C is added 66 over 0.5h a solution of bromine (4.05 mL, 78.2 mmol) in carbon tetrachloride (25 mL). The reaction is stirred 1h at 0*C, then 2h at room temperature. The solvents are removed under reduced pressure and the residue was short-path distilled at pump vacuum (about 0.5mm) . The fraction collected at 100*C-120*C provided 4-bromo-5-ethoxy-5H-furan-2-one (13.2 g, 82% yield) as a yellow oil. 1 H-NMR (500 MHz, CDCl 3 ) 8 6.24 (s, lH) , 5.63 (s, 1H) , 3.71 (m, 1H), 3.63 (m, 1H), 1.14 (t, J=7.1Hz, 3H) ppm. Pd-catalyzed Coupling of CBZ-Pro-NH 2 and Bromoethoxyfuranone [01291 To a 1L round bottom flask, CBZ-Pro-NH 2 (20 g, 80.4 mmol), Pd(OAc) 2 (0.36 g, 1.6 mmol), XANTPHOS (1.4 g, 2.4 mmol) was charged. The system was purged with nitrogen gas for 10 min. Toluene was added (200 mL), and the reaction was stirred with warming to 50 *C. After reaching 50 "C, the reaction was stirred for 30 min. The mixture changed from a yellow slurry to a brick-red solution as the aide dissolved and the (XANTPHOS)Pd(OAc) 2 complex formed. A solution of K 2 C0 3 (26.6 g, 192 mmol) in water (200 mL) was added and the reaction was allowed to warm to 50 C. [01301 To a beaker, bromoethoxyfuranone (18.3 g, 88.4 mmol) and toluene (30 mL) was charged. The reaction is stirred until a solution is formed (slight warming may be necessary because the dissolution is endothermic). The solution of the bromide is added slowly to the catalyst/amide solution at 50 *C over 3-3.5 hr. After the addition was complete, stirring of the reaction mixture was continued at 50 *C for 4 hours. While still at 50 OC, the phases were separated and the aqueous phase discarded. The organic phase was washed with water (100 mL) at 50 *C. The phases were separated and the aqueous phase discarded. The organic phase was concentrated to M volume and cooled to ambient temperature. Seeds were added (50 mg) if crystallization has not begun. The mixture was stirred at ambient temperature for 67 15 hr (overnight), cooled to 0 *C and stirred for 3-5 hr. The solid was filtered and rinsed with cold toluene. The solid was dried in vacuo at 40-50 0 C to give a white crystalline solid (10.8 g, 36% yield). [0131] In an alternative synthesis, a flask was charged with Pd 2 (dba) 3 (4.18 g, 4.6 mmol), Xantphos (7.90 g, 13.7 mmol), CBZ Proline amide (50 g, 201 mmol), Cs 2

CO

3 (65.5 g, 201 mmol) and toluene (770 mL). The mixture was stirred at 35 *C for 30 min, to give a brown/yellow mixture. [0132] Bromoethoxyfuranone (41.7 g, 201 mmol) as a solution in 30 mL toluene was added to the brown/yellow mixture. The solution was warmed to 80 *C. After 15 min, HPLC analysis showed 90% reaction complete (comparing CBZ-proline amide and product), and no bromoethoxyfuranone remained. Another 4.1 g of bromoethoxyfuranone was added to the reaction mixture at 85 *C. After stirring for 30 min, HPLC analysis showed 97% reaction completion. Another 2.8 g of bromoethoxyfuranone was added. After stirring for 45 min, HPLC analysis showed no CBZ-proline amide remaining. The mixture was cooled to 20-25 *C, and water (200 mL) was added, followed by saturated aqueous sodium hydrogen sulfate (400 mL). Gas evolution was observed. The phases were separated and the organic phase was washed with saturated aqueous sodium hydrogen sulfate, then water. The organic phase was dried over sodium sulfate, filtered, and the solvent was removed in vacuo. The resulting crude material was purified by flash chromatography (1:1 EtOAc:hexanes, then 3:1 EtOAc:hexanes) to give 55.7 g (74% yield) of the desired product as a light brown oil. [01331 H-NMR (d 6 -DMSO) : 510.20 (s, 0.5 H); 10.00 (s, 0.5 H); 7.55 (br s, 5H); 6.35 (s, 1H); 5.85 (s, 0.5H); 5.70 (s, 0.5H); 5.30 (m, 2H); 4.60 (br s, 1H); 4.05 (m, 1H); 3.85 (m, 1H); 3.65 (m, 1H); 3.55 (m, 1H); 2.05 (m, 4H); 1.40 (m, 3H). 68 Example 2 O N'O H 0 2 [0134] To a flask was charged the crude product produced as described above (37.36 g, 0.1 mol) and toluene (187 mL) . The mixture was stirred to give a beige/brown solution. Seeds of compound 2 (226 mg) were added and the mixture was stirred at ambient temperature for 3 days, at 0-5 *C for 8 hr, then at ambient temperature for another 7 days. The solution was cooled again to 0-5 *C and stirred for 3 hr, filtered, and the solid was rinsed with toluene. The solid was dried in the air to give 5.61 g (15% yield) of the title compound as a 97:3 mixture of anomers. 1 H-NMR (d,-DMSO): 5 7.35-7.25 (m, 5H); 5.75 (d, 1H); 5.70 (d, 1H); 5.1-4.9 (m, 2H); 4.35 (m, 1H); 3.70 (m, 1H); 3.60 (m, 1H); 3.40 (m, 2H) ; 2.15 (m, 1H)1.80 (m, 2H); 1.20 (t, 1.5 H) ; 1.10 (t, 1.5H) Example 3 0 O O N N 0O 0 N' H 0 (0135] To a flask was charged the compound described in Example 2 (5. 00 g, 13.3 mmol), 20% Pd (OH) 2 /C (1. 00 g, 50% wet) , isopropyl acetate (30 mL), and DMF (10 mL). The mixture was hydrogenated under 50 psig H 2 at 0-5 *C for 5 hr, then at ambient temperature for 21 hr. HPLC analysis showed the reaction to be 97% 69 complete. The mixture was filtered through celite and the solids were rinsed with a 3:1 isopropyl acetate:DMF solution to provide the unprotected compound of example 2. [0136] To Cbz-t-leu-OH dicyclohexylamine salt was added isopropyl acetate (30 mL) and 1.0 M H 2

SO

4 (30 mL). The mixture was agitated until two clear phases were obtained. The aqueous phase was discarded and the organic phase was washed with water (30 mL). The organic phase was collected. To the organic phase was added DMF (10 mL), then hydroxybenzotriazole (2.2 g, mmol). EDC (2.8 g) was added and the mixture was stirred for 1 hr. To this mixture was added the above hydrogenation solution. The mixture was stirred at ambient temperature for 8.5 hr. Water (100 mL) was added and the mixture was stirred for 1 hr. The phases were separated and the organic phase was washed with aqueous 0.5 M NaHSO 4 , saturated aqueous sodium chloride, and water. The solution was concentrated to dryness to give 4.04 g (62% yield) of the title compound. [0137] Alternatively, a 2 liter Parr pressure reactor was charged with 100.0 g (0.267 moles) a compound described in Example 2, and 10.0 g of 10% Pd/C (50% wet) . The reactor was purged with nitrogen for 10 minutes. 800.0 mL of ethyl acetate, followed by 19.5 mL of trifluoroacetic acid were then added. The reactor was then closed, pressurized to 60 psi with hydrogen followed by venting. This cycling was repeated twice. The reaction was stirred for 2 hours under hydrogen (60 psi). The palladium catalyst was filtered through a pad of celite, and the filtrate was held at 4 *C until needed for the subsequent coupling step. [01381 To a 3 liter, 3-neck round bottom flask equipped with mechanical stirring and a thermocouple was charged 43.3 g of 1 Hydroxybenzotriazole (anhydrous, 0.320 moles). To this flask was added a solution of Cbz-t-leucine (70.8 g in 430 mL of EtOAc). DMF (190 mL was charged to this suspension, and a clear 70 light yellow solution was achieved. To this solution was charged 1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride (EDC, 56.3 g, 0.294 moles). A thin suspension formed, and was stirred for 2 hours at 22 0 C. To this suspension was charged the solution of the unprotected compound of example 2 (TFA salt) . Triethylamine (112 mL, 0.801 moles) was added dropwise over 30 minutes, and the resulting suspension was stirred at 22 *C for 2 hours. Water (400 mL) was added, and the biphasic mixture was stirred for 12 hours at 22 0 C. This biphasic mixture was then transferred to a 4-liter separatory funnel, and the aqueous layer was removed. The organic layer was washed with 400 mL of saturated sodium bicarbonate solution followed by water (2 X 400 mL). The ethyl acetate was distilled under vacuum to a final volume of approximately 400 mL. To this crude solution was charged 200 mL of heptane, followed by seeding with 1.0 g of the compound of example 3. The cloudy suspension was then cooled to 5 *C, which resulted in the formation of a thick slurry. Additional heptane was charged (400 mL) over a three hour period while maintaining the batch at 5 *C. The solids were isolated by vacuum filtration, rinsing the filter cake with a 2:3 EtOAc/Heptane mixture (2 X 100 mL). The solids were dried for 12 hours in a vacuum oven at 22 0 C, with a nitrogen bleed (80% yield for 2 steps). [0139] 1 HNMR (CDCl 3 ) 6 7.5 (d, 1H, J = 7.8 Hz); 7.4-7.3 (m, SH); 5.5 (overlapping d, m, 2H, J = 5.3 Hz) ; 5.1 (d, 1H, J = 12.3 Hz); 5.1 (d , 1H, J = 12.2 Hz); 4.7-4.6 (m, 2H); 4.4 (d, 1H, J 9.7 Hz); 3.9 (m, 1H); 3.8 (q, 1H, J = 8.4 Hz); 3.7-3.6 (m, 2H); 2.8 (dd, 1H, J = 17.2, 8.4 Hz); 2.4-2-3 (overlapping m, dd, 2H, J = 17.2, 10.4 Hz); 2.1 (m, 1H); 2.0 (m, 1H); 1.9 (m, 1H); 1.3 (t, 3H, J = 7.2 Hz); 1.0 (s, 9H). 71 Example 4: Scheme 3 and Synthetic Examples Scheme 3 H 0 H H Pd/C, HO 2

CCO

2 H IHHO 2 CCO H 0 0 0a HON N + H 2 N OH Et /2 H 3N RO 0

HO

2 N40_; 0 aio- 75-85% H 2 N( [01401 The compound described in Example 3 may be further modified by removing the protecting group and coupling additional moieties to the Leucine amine. Example 5: Scheme 4 and alternative Procedures Scheme 4 0 N-zk 0 H 2 /Pd/C/HCOOH HN O H O- HN O EDC /Cbz-t-leu 0 0 0 O r J O O NN H O O HN O N4 H 0O 72 [01411 To a 1-liter, 3-neck round bottom flask equipped with mechanical stirring and a nitrogen inlet was charged 50.0 g of the compound of example 2 (0.134 moles), and 10.0 g of 10% Pd/C (50% wet) . The vessel was purged with nitrogen for 10 minutes. Formic acid (500 mL) was added, and the suspension was stirred under nitrogen for 16 hours at 22 0 C. The reaction mixture was filtered through celite, and to the filtrate was added 20.6 mL of trifluoroacetic acid. The formic acid was distilled under vacuum, and the remaining formic acid was removed by azeotropic distillation with toluene. The crude oil that was obtained was dissolved in 150 mL of ethyl acetate, and methyl-tert-butyl ether (100 mL) was charged dropwise over 2 hours to crystallize the trifluoroacetate salt. The suspension was cooled to 5 0 C, and the solids were collected by vacuum filtration, rinsing with a 3:2 EtOAc/MTBE solution (2 X 50 mL) to furnish the desired product as a TFA salt in 55% yield. [01421 1 HNMR (ds-DMSO) 511.6 (br. s, 1H); 9.1 (br. s, 2H); 6.15 (s, 1H); 6.05 (s, 1H); 4.5 (m, 1H); 3.75 (m, 2H); 3.3 (m, 2H); 2.35 (m, 1H); 1.95 (m, 3H); 1.2 (t, 3H, J = 6.7 Hz) 0 O Ok O N 0 H 0O -\ [01431 To a 1-liter, 3-neck round bottom flask equipped with mechanical stirring, addition funnel and nitrogen inlet was charged a solution of Z-tert-Leucine (20.6 g, 0.0776 moles) in dichloromethane (250 mL). Anhydrous 1-Hydroxybenzotriazole (10.5 g, 0.0776 moles) was added to this solution, followed by 14.9 g of 1- [3- (Dimethylamino)propyl] -3-ethylcarbodiimide (EDC, 0.0776 moles) . A homogenous solution was achieved, and was stirred for 2 hours at 22 0 C. To this reaction was charged 25.0 73 g of the unprotected praline analog (TFA salt, 0.0706 moles), followed by 4-methylmorpholine (15.5 mL, 0.141 moles). The solution was stirred for 3 hours at 22 'C. The reaction mixture was transferred to a separatory funnel, and washed with aqueous saturated sodium bicarbonate (100 mL) , followed by a 10% aqueous solution of citric acid (100 mL). The organic layer was purified by silica gel chromatography (50% EtOAc/Hexane) to afford the desired product in 60% yield. 101441 1 HNMR (d 6 -DMSO) 611.0 (s, 1H); 7.35 (m, 5H); 7.25 (d, 1H); 6.0 (br. s, 2H); 5.1 (d(ab), 1H); 5.0 (d(ab), 1H); 4.5 (br. s, 1H); 4.2 (d, 1H); 3.8 (m, 3H); 3.65 (m, 1H); 2.15 (m, 1H); 1.9 (m, 2H); 1,8 (m, 1H); 1.2 (t, 3H); 1.0 (s, 9H). Scheme 6 and Synthetic Examples Scheme 5 STEP 1: 1% Pd(OAc) 2 , 1.5% XantPhos O 2 eq. K 2 C0 3 . 3 eq. H 2 0 0 0 AI(OEt) 3 , HCI0 2% Cetylrimethylammonium Br O\ Al(Toluee CbzN . + O CbzN N P . Cbz N N O Cbz' N N 4 S B OEt H Ot H OEt 0 H Ot STEP 2 0 H2, PdC 0 Cbz-tert-Leu-OH O bz N 1 0 TFA HN EDC, HOBt Cbz N 0 H Ot 0 H OEt 0 OEt STEP 3: 0 H0 1 11 OH0 Cb rNI N Hc O t PdC d H 2 NNE H 2 NOH2 N O t HlfrCitric Acid a ~ Ot CM 2 Cb z'N N0 0 O H OEt 74 74 [0145121.7 ml of water was added to a mixture of 100.0 g of CBZ prolinamide, 0.92g of Palladium acetate, 3.47g of Xantphos, 111.2g of Potassium carbonate and 2.93g of Cetyltrimethylammonium bromide in Toluene (1000 ml) maintaining the temperature at T = 20-25 0 C. All vessel chargings and additions were performed under nitrogen to avoid/limit oxidation of the Palladium catalyst. The reaction was then warmed to T=50-550C and-stirred-for-about-2--hours.-Separat-ly, Bromoethoxyfuranone (91.5 g) and toluene (100 ml) were charged into a separate flask and stirred at 20-25 0 C until complete dissolution occurred. The Bromoethoxyfuranone solution was then added to the initial reaction mixture over 3-3.5 hours at 50 550C and then stirred until the reaction was completed in quantitative yield in about 1 hour. The reaction mixture was filtered at T = 50-55*C and the solids were rinsed with Toluene (500 ml). The filtrate was washed with water(500 ml. The aqueous phase was discarded and the organic phase was concentrated to approximately 500 ml at <50 0 C under vacuum. The solution was cooled to 5 0 C-10*C and 9.8 g Aluminum triethoxide were added. [01461 Into a separate flask 11.3 ml Acetyl chloride was added to a solution made of 100 ml Toluene and 9.7 ml Ethanol, maintaining the temperature at T = 5-10 0 C (in situ generation of anhydrous HCl), then the mixture was stirred at T = 5-10*C for about 1 hour. The Toluene/Ethanol/HCl solution was then added to the previous reaction mixture over 15 minutes at T =5-10*C, then seeded with the product and stirred at T = 5-10 0 C for 12 hours, at T = 20-25*C for 48 hours, at T = 5-10*C for 12 hours. The product was filtered at T = 5-10 0 C and washed with 100 ml of Toluene. The wet material was dissolved at 70OC-754C) in 1500 ml Toluene and the solution was filtered at 754C through Dicalite (filtration aid agent). The solids were rinsed with 75 100 ml Toluene. The organic solution wras vacuum concentrated to 500 ml. The resultant slurry was cooled to 20-25*C over 1 hour, stirred for 3-4 hours, filtered and the product rinsed with 100 ml toluene. The product was dried under vacuum at 35-40*C. STEP 2: C Cbz' N N H O H OEt [0147] The furanone of Step 1 100 g was charged into a stainless steel (3 lt) autoclave together with 20 g of 5%Palladium on charcoal (approx. 50% wet) , followed by 800 ml of ethyl acetate and 19.5 ml of trifluoroacetic acid. The autoclave was pressurized with hydrogen (4 bars) and the temperature set at T = 20-25 0 C. The hydrogenolysis was run for 2-3 hrs, periodically repressurizing to 4 bar as hydrogen uptake proceeds, until upptake of hydrogen ceased. The catalyst was filtered off and washed twice with 100 ml of ethyl acetate to give a solution of the deprotected proline compound. [0148] Separately, a solution of sulfuric acid (14.6 ml) in water (330 ml) was added to a mixture of 119. 2 g of Cbz-t-leucine dicyclohexylamine salt and 430 ml of Ethyl acetate. The resulting solution was stirred at T =20 -25 0 C for 30 minutes. The organic layer was separated, washed twice with 500 ml of water and added to 43.3 g of hydroxybenzotriazole. DMF (190 ml) was added to this mixture followed by 5 6.3 g of EDC which produced a cloudy reaction mixture from the clear yellowish solution. The reaction was stirred at 7 = 20-25 0 C for 30-60 minutes. The solution of deprotected proline compound from the autoclave was charged to the reaction mixture, 81.1 g of Triethylamine was then added dropwise (over 20-30 minutes) and the resulting cloudy mixture was stirred at T = 20-25 0 C for 1.5 76 2 hours. 400 ml of water was added and the reaction stirred at 20-25*C for 12 hours. The organic layer was separated and washed with 400 ml of an aqueous sodium bicarbonate (7.5%) solution and twice with 400 ml of water. These water washings were performed at 45-50 0 C. The organic phase was concentrated to 400 ml volume at 40-451C. 300 ml of ethyl acetate were added and the mixture concentrated to 350 ml to remove residual water. The solution was cooled to 20-250C and 200 ml of N-heptane added over 1 hour at 20-25 0 C, and the mixture seeded with the compound shown in Example 3 above and stirred at T = 20-25 0 C for 1 hour. The resultant slurry was cooled to T = 5-10 0 C and stirred for an additional hour at the same temperature. 400 ml of N-Heptane were added over 2-3 hours at T = 5-10*C, the slurry was filtered and rinsed twice with Ethyl acetate/N-heptane (40 ml, 60 ml respectively). The crystals were dried under vacuum at T = 35 40 0 C for at least 8 hours. STEP 3: 0 0 N N H 0 N

H

2 N O H OEt CI [0149]The product of step 2 (100 g), 5%Palladium on charcoal (approx. 50% wet, 20 g) 100 ml of DMF, 600 ml of ethyl acetate and 43.1 g of Citric acid monohydrate were charged into a stainless steel (3 lt) autoclave. The stainless steel autoclave was pressurized with hydrogen (4 bar) and the temperature set at -2* to +20C. The reaction was run for 2-3 hrs periodically repressurizing to 4 bar as hydrogen uptake proceeds. The catalyst was filtered off and washed with a mixture of 85 ml of ethyl acetate and 15 ml of DMF. [0150] Separately, 23.5 g of N-Methylmorpholine is added to a mixture of 33.1 g of 4-Amino-chloro-benzoic acid 34.4 g of 2 77 Chloro-4,6-dimethoxytriazine (DMT-Cl) in 300 ml of ethyl acetate over 20-30 minutes at ambient temperature for 2-3 hours at 23 27 0 C to obtain the DMT active ester of 4- Amino- 3-chlorobenzoic acid. The mixture is cooled to 00 to +5*C and 30 0 ml of purified water are added to the solution keeping temperat-ure in the same range. The solution of the deprotected t-leucirae product as the citrate salt is added at 0 0 C to +5 0 C over 30-60 minutes, the reaction mixture is then brought to pH 6.5-7.5 tDy adding 30% sodium hydroxide (approx. amount: 71 ml), and stirred 6-7 hrs at 200 to 25 0 C. After completion of the reaction, the phases are separated and the organic layer added to sodium bisulfate solution (15 g of sodium bisulfate in 235 ml of water) and stirred for 3 hrs at 20 0 C to 25 0 C. The phases a:ce separated and the organic layer is washed four times with wateEr (150 ml each), twice with sodium bicarbonate solution (total: 20 g of sodium bicarbonate in 400 ml of water), and once with 1.50 ml of water. To the solution is added 10 g of activated charcoal and 10 g of Dicalite and filtered and the solids washed withi. 100 ml of ethyl acetate. The filtrate was distilled under vacuiam to a volume of 200 ml at < 40 0 C when the resultant mixture crystallizes. Ethyl acetate (150 ml) was added to a total volume of 350 ml. N heptane (300 ml) was added over 2 hrs and after stirring the slurry for 3 hrs at 200 to 250 , the solid was f iltered, washed with ethyl acetate/N-Heptane (100 ml, 1:1) and cried at 60 0 C under vacuum. [01511 All of the documents cited herein are here1>y incorporated herein by reference. [01521 While we have described a number of embodiments of this invention, it is apparent that our basic examples may be altered to provide other embodiments which utilize the compounds and methods of this invention. For instance, protecting groups besides CBZ may be used to protect amines in one5 or more of the 78 synthetic steps described above. Therefore, it will be appreciated that the scope of this invention is to be defined by the appended claims rather than by the specific embodirmients which have been represented by way of example. 79

Claims (6)

1. A process of producing a compound of 0 R 8 -N H N |0 O-R 9 5 comprising purifying a mixture of 0 R 8 -N H NI 0 O-R 9 10 wherein R 8 is a protecting group and R 9 is Ci 5 alkyl, and purifying includes selectively crystallizing the mixture with an organic solvent, or dynamically crystallizing the mixture wherein: selective crystallization comprises combining the mixture and an organic solvent at or above room temperature and warming the combination with stirring to dissolve the 15 mixture and then cooling the combination; and wherein: dynamically recrystallizing the mixture comprises contacting the mixture with a Lewis acid and a solvent optionally including a protic acid; wherein: the organic solvent is selected from toluene, dioxane, THF, or mixtures thereof; the lewis acid is selected from BF 3 -etherates, metal halides, alkoxides, or mixed 20 halide/alkoxides; the mixed halide alkoxides are selected from Al(Oalkyl) 2 C1 or Al(Oalkyl)Cl 2 ); the alkyl group of the alkyl or alkoxide is a straight or branched chain having one to twelve carbon atoms; the metal is aluminum, titanium, zirconium, magnesium, copper, zinc, iron, tin, 25 boron, ytterbium, lanthanum, or samarium; and the protic acid is selected from HCl, HBr, triflic acid, sulfuric acid, phosphoric acid, or combinations thereof.

2. The process of claim 1, wherein the step of purifying the mixture comprises selectively crystallizing the mixture with an organic solvent. A1Tfl1 /0Ti6l 1 \.TTD - 81

3. The process of claim 1, wherein the step of purifying comprises dynamic crystallization which comprises contacting the mixture with a lewis acid and a solvent optionally including a protic acid.

4. The process of claim 3, wherein the step of purifying comprises contacting the 5 mixture with AL(Oalkyl) 3 in a solvent including a protic acid.

5. The process of claim 4, wherein the mixture is epimerized with Al(OEt) 3 in toluene in the presence of HCl.

6. The process of claim 2, wherein the organic solvent is toluene. Vertex Pharmaceuticals Incorporated 10 Patent Attorneys for the Applicant/Nominated Person SPRUSON & FERGUSON AUTJ11/0 %A(~1 \TTD