AU2011205049A1 - Use of EDG receptor binding agents in cancer - Google Patents

Abstract

Provided Is a method for treating solid tumors, e.g tumor Invasiveness, and particularly inhibiting or controlling deregulated angiogenesis, using a sphingosine-1 -phosphate receptor agonist, optionally in combination with a chemotherapeutic agent. The invention also comprises a combination of a sphingosine-1-phosphate receptor agonist with a chemotherapeutic agent.

Description

Australian Patents Act 1990 - Regulation 3.2A ORIGINAL COMPLETE SPECIFICATION STANDARD PATENT Invention Title "Use of EDG receptor binding agents in cancer" The following statement is a full description of this invention, including the best method of performing it known to us: C\NRPortbl\DCC\DAR\3780120 I.DOC - 1 Use of EDG receptor binding agents In cancer This application is a divisional of Australian Patent Application No. 2007237378, the entire content of which is incorporated herein by reference. The present Invention relates to a new use for a sphingosine-1 -phosphate (S1P) receptor agonist, particularly in the treatment of cancer. S1 P receptor agonists are accelerating lymphocyte homing (LH) agents which elIcit a lymphopenia resulting from a re-distribution, preferably reversible, of lymphocytes from circulation to secondary lymphatic tissue, without evoking a generalized immunosuppression. Naive cells are sequestered; CD4 and CD8 T-cells and B-cells from the blood are stimulated to migrate into lymph nodes (LN) and Peyer's patches (PP), and thus for example infiltration of cells into transplanted organs is inhibited. S1 P receptor agonists are typically sphIngosine analogues, such as 2-substituted 2-amino propane-1,3-diol or 2-amino-propanol derivatives, e.g. a compound comprising a group of formula X Z x

R

3 zR2zN CH 2 RIZ wherein Z Is H; C 1

.

4 alkyl; C 2 -salkenyl; C 2

.

4 alkynyl; phenyl; phenyl substituted by OH; C 1 ealkyi substituted by 1 to 3 substituents selected from the group consisting of halogen, C 3 . 8 cycloalkyl, phenyl and phenyl substituted by OH; or CH 2

-R

4 z wherein R 4 z is OH, acyloxy or a residue of formula (a)

OR

5 Z -- OR (a) 0 6Z wherein Z 1 Is a direct bond or 0, preferably 0; each of R&. and Re,, independently, is H, or

C

1

.

4 alkyl optionally substituted by 1, 2 or 3 halogen atoms; Ra Is OH, acyloxy or a residue of formula (a); and each of R2z and Ra, independently, is H,

C

1

.

4 alkyl or acyl. Group of formula X is a functional group attached as a terminal group to a moiety which may be hydrophilic or lipophilic and comprise one or more aliphatic, alicyclic, aromatic and/or heterocyclic residues, to the extent that the resulting molecule wherein at least one of Z and Ri Is or comprises a residue of formula (a), signals as an agonist at one of more sphingosine-1 -phosphate receptor.

-2 S1P receptor agonists are compounds which signal as agonists at one or mono sphingosine 1 phosphate receptors, e.g. S1 P1 to S1 P8. Agonist binding to a Si P receptor may e.g. result in dissociation of intracellular heterotrimeric G-proteins into Ga-GTP and Gy-GTP, and/or increased phosphorylation of the agonist-occupied receptor and activation of downstream signaling pathways/kinases. The binding affinity of S1 P receptor agonists may be measured as described at paragraph I. below. Examples of appropriate S1P receptor agonists are, for example: - Compounds as disclosed in EP627406A1, e.g.a compound of formula I H ORa

R

4 RN-4CH 2

OR

2 R, wherein R 1 is a straight- or branched (C 12 .22)carbon chain - which may have in the chain a bond or a hetero atom selected from a double bond, a triple bond, 0, S, NRO, wherein R 6 is H, alkyl, aralkyl, acyl or alkoxycarbonyl, and carbonyl, and/or - which may have as a substituent alkoxy, alkenyloxy, alkynyloxy, aralkyloxy, acyl, alkylamino, alkylthio, acylamino, alkoxycarbonyl, alkoxycarbonylamino, acyloxy, alkylcarbamoyl, nitro, halogen, amino, hydroxyimino, hydroxy or carboxy; or

R

1 is - a phenylalkyl wherein alkyl is a straight- or branched (CS.20)carbon chain; or - a phenylalkyl wherein alkyl is a straight- or branched (C 1 .s)carbon chain wherein said phenylalkyl is substituted by - a straight- or branched (C- 20 )carbon chain optionally substituted by halogen, - a straight- or branched (Ce.2o)alkoxy chain optionally substitued by halogen, - a straight- or branched (C 6 .20)alkenyloxy, - phenylalkoxy, halophenylalkoxy, phenylalkoxyalkyl, phenoxyalkoxy or phenoxyalkyl, - cycloalkylalkyl substituted by C.

2 Dalkyl, - heteroarylalkyl substituted by Cs.2oalkyl, - heterocyclic Cr-2Dalkyl or - heterocyclic alkyl substituted by C 2 .2oalkyi, and wherein the alkyl moiety may have - in the carbon chain, a bond or a heteroatom selected from a double bond, a triple bond, 0, S, sulfinyl, suffonyl, or NR 6 , wherein R 6 is as defined above, and -3 - as a substituent alkoxy, alkenyloxy, alkynyloxy, aralkyloxy, acyl, alkylamino, alkylthio, acylamino, alkoxycarbonyl, alkoxycarbonylamino, acyloxy, alkylcarbamoyl, nitro, halogen, amino, hydroxy or carboxy, and each of R 2 , R 3 , R 4 and R 5 , independently, is H, C14 alkyl or acyl or a pharmaceutically acceptable salt thereof; - Compounds as disclosed in EP 1002792A1, e.g. a compound of formula 11

H

2 0R' 3 0

R'

4 R'sN-C-(CH 2 ) -- (C 2)

CH

2 0R' 2 wherein m is 1 to 9 and each of R' 2 , R' 3 , R' 4 and R's, independently, is H, alkyl or acyl, or a pharmaceutically acceptable salt thereof; - Compounds as disclosed in EP0778263 Al, e.g. a compound of formula Ill NR", R"2 W -C -Z2 (C H2),,O R "a III wherein W is H; C 1 .6alkyl, C 2 .6alkenyl or C 2 -ealkynyl; unsubstituted or by OH substituted phenyl; R" 4 0(CH 2 )n; or C 1 .6alkyl substituted by 1 to 3 substituents selected from the group consisting of halogen, C3- 9 cycloalkyl, phenyl and phenyl substituted by OH; X is H or unsubstituted or substituted straight chain alkyl having a number p of carbon atoms or unsubstituted or substituted straight chain alkoxy having a number (p-1) of carbon atoms, e.g. substituted by 1 to 3 substitutents selected from the group consisting of C 1 . alkyl, OH,

C

1 .6alkoxy, acyloxy, amino, C 1 .6alkylamino, acylamino, oxo, haloC 1

.

4 alkyl, halogen, unsubstituted phenyl and phenyl substituted by 1 to 3 substituents selected from the group consisting of C 1 .6alkyl, OH, C 1 .6alkoxy, acyl, acyloxy, amino, C 1 .6alkylamino, acylamino, haloC 1 .-alkyl and halogen: Y is H, C 1 .- alkyl, OH, C 1 .6alkoxy, acyl, acyloxy, amino, C 1 . Galkylamino, acylamino, haloC 1 .6alkyl or halogen, Z 2 is a single bond or a straight chain alkylene having a number or carbon atoms of q, each of p and q, independently, is an integer of 1 to 20, with the proviso of 6sp+qs23, m' is 1, 2 or 3, n is 2 or 3, each of R" 1 , R" 2 , R" 3 and R" 4 , independently, is H, C 1 .4alkyl or acyl, or a pharmaceutically acceptable salt thereof, - 4 Compounds as disclosed in W002/18395, e.g. a compound of formula IVa or IVb 9H12R3b Ria 9H2Raa RITI I (R 2

.)

2

N-C-CH

2 -X7- P =0

(R)

2 NHC-C-X-- P =0 1 IIH Nib Rib 2 or H2 2

(CH

2

)

7

CH

3 IVa Ya-R 4 a IVb wherein Xa is 0, S, NR 1 , or a group -(CH 2 ),,-, which group is unsubstituted or substituted by 1 to 4 halogen; na is 1 or 2, R 1 , is H or (C 1

.

4 )alkyl, which alkyl is unsubstituted or substituted by halogen;

R

1 , is H, OH, (C 1 .4)alkyl or O(C 1 .4)alkyl wherein alkyl Is unsubstituted or substituted by 1 to 3 halogen; Rib is H, OH or (C 1 .4)alkyl, wherein alkyl is unsubstituted or substituted by halogen; each R 2 a is independently selected from H or (C 1 .4)alkyl, which alkyl is unsubstituted or substitued by halogen; R 3 a is H, OH, halogen or O(C 1 .4)alkyl wherein alkyl is unsubstituted or substituted by halogen; and R3b is H, OH, halogen, (C 1

.

4 )alkyl wherein alkyl is unsubstituted or substituted by hydroxy, or O(C 1 .4)alkyl wherein alkyl is unsubstituted or substituted by halogen; Y. is -CH 2 -, -C(O)-, -CH(OH)-, -C(=NOH)-, 0 or S, and R 4 . is (C 4 . 1 4 )alkyl or (C 4

.

1 4)alkenyl; or a pharmaceutically acceptable salt or hydrate thereof; - Compounds as disclosed in WO 02/076995, e.g. a compound of formula V Rc

R

4 ,RaCN (CH 2 )m,-XR 2 e wherein m. is 1, 2 or 3; X, is 0 or a direct bond;

R

1 is H; C1.6 alkyl optionally substituted by OH, acyl, halogen, Ca.

10 cycloalkyl, phenyl or hydroxy-phenylene;

C

2 -6alkenyl; C 2 -ealkynyl; or phenyt optionally substituted by OH;

R

2 e is - 5 __ p<O0sc jj ON.~ 0 wherein R 5 , is H or C 1 .4alkyl optionally substituted by 1, 2 or 3 halogen atoms, and Rf is H or C 1

.

4 alkyl optionally substituted by halogen; each of Ra and R4,, independently, is H, C 1

.

4 alkyl optionally substituted by halogen, or acyl, and R. Is C 1 a-2oalkyl which may optionally have in the chain an oxygen atom and which may optionally be substituted by nitro, halogen, amino, hydroxy or carboxy; or a residue of formula (a)

R

7 -(CH2)2-4- R,, (a) wherein R 7 . is H, C 1 .4alkyl or C 1

.

4 alkoxy, and Re is substituted C 1

.

20 alkanoyl, phenylC 1

.

14 alkyl wherein the C 1

.

1 4 alkyl is optionally substituted by halogen or OH, cycloalkylC 1

.

4 alkoxy or phenylC 1

.

14 alkoxy wherein the cycloalkyl or phenyl ring is optionally substituted by halogen, C 1

.

4 alkyl and/or C 1

.

4 alkoxy, phenylC 1

.

14 alkoxy

C

1

.

4 alkyl, phenoxyC 1

.

14 alkoxy or phenoxyC,.

1 4 alkyl, R, being also a residue of formula (a) wherein Rae is C 1

.

14 alkoxy when R 1 , is C 1 .4alkyl, C2.6alkenyl or C 2 .ealkynyl, or a compound of formula VI RR RU Ra3x N CH2 )n

CH

2

OR

2 x 6x VI wherein nx Is2,3or4

R

1 . is H; C 1 .6alkyl optionally substituted by OH, acyl, halogen, cycloalkyl, phenyl or hydroxy-phenylene; C 2 -6alkenyl; C 2 -ealkynyl; or phenyl optionally substituted by OH; R2x is H, C1.4 alkyl or acyl each of R 3 , and R 4 X, independently is H, C 1

.

4 alkyl optionally substituted by halogen or acyl, Rs is H, C 1 .4alkyl or C 1

.

4 alkoxy, and - 6 R6, is C1.20 alkanoyl substituted by cycloalkyl; cyloalkylC 1 .1 4 alkoxy wherein the cycloalkyl ring is optionally substituted by halogen, C 1 .4alkyl and/or C 1

.

4 alkoxy; phenylC 1 .14alkoxy wherein the phenyl ring is optionally substituted by halogen, C 1

.

4 alkyl and/or C 1 4 alkoXy, R6, being also C 4

.

1 4 alkoxy when R 1 , Is C2.4alkyl substituted by OH, or pentyloxy or hexyloxy when R1, is C 1

.

4 akyl, provided that R, Is other than phenyl-butylenoxy when either R 5 . is H or R 1 , is methyl, or a pharmaceutically acceptable salt thereof; - Compounds as disclosed in W002/06268AI, e.g. a compound of formula VIl NR dR 2 d :Kl~ d

R

4 d (CH 2 )nR X---Y -R 8 R 3d 0 V11 wherein each of Rid and R2d, independently, is H or an amino-protecting group; Ra is hydrogen or a hydroxy-protecting group; R4d is lower alkyl; nd is an integer of 1 to 6; Xd is ethylene, vinylene, ethynylene,.a group having a formula - D-CH 2 - (wherein D is carbonyl, - CH(OH)-, 0, S or N), aryl or aryl substituted by up to three substitutents selected from group a as defined hereinafter; Yd is single bond, C 1

.

10 alkylene,

C

1

.

10 alkylene which is substituted by up to three substitutents selected from groups a and b, C 1

.

10 alkylene having 0 or S in the middle or end of the carbon chain, or C 1

.

10 alkylene having 0 or S in the middle or end of the carbon chain which is substituted by up to three substituents selected from groups a and b; Rsd is hydrogen, cycloalkyl, aryl, heterocycle, cycloalkyl substituted by up to three substituents selected from groups a and b, aryl substituted by up to three substituents selected from groups a and b, or heterocycle substituted by up to three substituents selected from groups a and b; and each of Red and R7d, independently, is H or a substituent selected from group a; <group a> is halogen, lower alkyl, halogeno lower alkyl, lower alkoxy, lower alkylthlo, carboxyl, lower alkoxycarbonyl, hydroxy, lower aliphatic acyl, amino, mono-lower alkylamino, di-lower alkylamino, lower aliphatic acylamino, cyano or nitro; <group b > is cycloalkyl, aryl, heterocycle, each being optionally substituted by up to three substituents selected from group a; -7 with the proviso that when Rsd is hydrogen, Yd is a either a single bond or linear C 1

.

1 0 alkylene, or a pharmacologically acceptable salt or ester thereof. -Compounds as disclosed in JP-1 4316985 (JP2002316985), e.g. a compound of formula VilI:

NR

1 0

R

2 6 Roe X-Y-R 5 e

R

4 , (CH 2 )n 8 39 0R7 ViO wherein

R

1

.,R

2

.,R

3

,R

4 .,Re.,Re,,R7,, n,, X. and Y. are as disclosed in JP-14316985; or a pharmacologically acceptable salt or ester thereof. -Compounds as disclosed In WO 03/29184 and WO 03/29205, e.g. compounds of formula Ix Ri , R 3 f NH 2 RX( CH 2 0H IX a (CHCH OH wherein Xf is 0 or S, and R 1 , R2f, R3, and n, are as disclosed in WO 03/29184 and 03/29205, e.g. 2 -amino-2-[4-(3-benzyloxyphenoxy)-2-chloropheny]propyl-1,3-propane-diol or 2-amino 2-[4-(benzyloxyphenylthio)- 2 - chlorophenyllpropyl-1,3-propane-diol. In each case where citations of patent applications are given, the subject matter relating to the compounds is hereby incorporated into the present application by reference. Acyl may be a residue Ry-CO- wherein Ry is C 1 salkyl, C 3 .cycloalkyl, phenyl or phenyl-C1. 4 alkyl. Unless otherwise stated, alkyl, alkoxy, alkenyl or alkynyl may be straight or branched. When in the compounds of formula I the carbon chain as R 1 is substituted, it is preferably substituted by halogen, nitro, amino, hydroxy or carboxy. When the carbon chain is interrupted by an optionally substituted phenylene, the carbon chain is preferably unsubstituted. When the phenylene moiety Is substituted, it is preferably substituted by halogen, nitro, amino, methoxy, hydroxy or carboxy. Preferred compounds of formula I are those wherein

R

1 is C 13

.

20 alkyl, optionally substituted by nitro, halogen, amino, hydroxy or carboxy, and, more preferably those wherein

R

1 Is phenylalkyl substituted by CO.

14 -alkyl chain optionally substituted by halogen and the alkyl moiety is a C 1 .6alkyl optionally substituted by hydroxy. More preferably,

R

1 is phenyl-C.alkyl substituted on the phenyl by a straight or branched, preferably straight, C 6

.

14 alkyl chain. The

C.

1 4 alkyl chain may be in ortho, meta or para, preferably in para.

- 8 Preferably each of R 2 to R, 5 is H. A preferred compound of formula I is 2-amino-2-tetradecyl-1,3-propanediol. A particularly preferred S1 P receptor agonist of formula I Is FTY720, i.e. 2-amino-2-[2-(4-octylphenyl) ethyl]propane-1,3-diol In free form or In a pharmaceutically acceptable salt form (referred to hereinafter as Compound A), e.g. the hydrochloride, as shown:

H

3 N HCI A preferred compound of formula |1 is the one wherein each of R' 2 to R's is H and m is 4, i.e. 2-amino-2-(2-[4-(1-oxo-5-phenylpentyl)phenyl]ethylpropane-1,3-diol, in free form or in pharmaceutically acceptable salt form (referred to hereinafter as Compound B), e.g the hydrochloride. A preferred compound of formula Ill is the one wherein W is CH 3 , each of R" 1 to R"a is H, Z 2 is ethylene, X is heptyloxy and Y is H, I.e. 2-amino-4-(4-heptyloxyphenyl)-2-methyl-butanol, in free form or in pharmaceutically acceptable salt form (referred to hereinafter as Compound C), e.g. the hydrochloride. The R-enantiomer is particularly preferred. A preferred compound of formula IVa is the FTY720-phosphate

(R

2 . is H, Ra is OH, X. is 0,

R

1 , and Rib are OH). A preferred compound of formula IVb is the Compound C-phosphate

(R

2 a is H, R 3 b is OH, X. is 0, Ri. and Rib are OH, Ya is 0 and Ra is heptyl). A preferred compound of formula V is Compound B-phosphate. A preferred compound of formula V is phosphoric acid mono-[(R)-2-amino-2-methyl-4-( 4 pentyloxy-phenyl)-butyl]ester. A preferred compound of formula Vill is (2R)-2-amino-4-[3-( 4 cyclohexyloxybuty)benzo[b]thien-6-y]-2-methylbutan-1 -ol. When the compounds of formulae I to IX have one or more asymmetric centers in the molecule, the present invention is to be understood as embracing the various optical isomers, as well as racemates, diastereoisomers and mixtures thereof are embraced. Compounds of formula IlI or IVb, when the carbon atom bearing the amino group is asymmetric, have preferably the R-configuration at this carbon atom. Examples of pharmaceutically acceptable salts of the compounds of the formulae I to IX include salts with inorganic acids, such as hydrochloride, hydrobromide and sulfate, salts with organic acids, such as acetate, fumarate, maleate, benzoate, citrate, malate, - 9 methanesulfonate and benzenesulfonate salts, or, when appropriate, salts with metals such as sodium, potassium, calcium and aluminium, salts with amines, such as triethylamine and salts with dibasic amino acids, such as lysine. The compounds and salts of the methods of the present invention encompass hydrate and solvate forms. The S1 P receptor agonists have, on the basis of observed activity, e.g. homing of lymphocytes, e.g. as described in EP627406A1 or USP 6,004,565, been found to be useful e.g. as immunosuppressant, e.g. in the treatment of acute allograft rejection. It has now been found that S1 P receptor agonists have interesting properties which make them useful for cancer chemotherapy, particularly of solid tumors, especially of advanced solid tumors. There is still the need to expand the armamentarium of cancer treatment of solid tumors, especially in cases where treatment with anticancer compounds is not associated with disease regression or stabilization. In accordance with the particular findings of the present invention, there is provided: 1.1 A method for treating solid tumors in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a SIP receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof. 1.2 A method for inhibiting growth of solid tumors in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a S1 P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof. 1.3 A method for inducing tumor regression, e.g. tumor mass reduction, in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a Si P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof. 1.4 A method for treating solid tumor invasiveness or symptoms associated with such tumor growth in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a S1 P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof. 1.5 A method for preventing metastatic spread of tumours or for preventing or inhibiting growth of micrometastasis in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a SiP receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof. 1.6 A method for inhibiting or controlling deregulated angiogenesis, e.g. sphingosine-1 phosphate (S1 P) mediated angiogenesis, in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a S1 P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof.

-10 1.7 A method for preventing or treating diseases mediated by a neo-angiogenesis process or associated with deregulated angiogenesis In a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a S1 P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof. By "solid tumors" are meant tumors and/or metastasis (whereever located) other than lymphatic cancer, e.g. brain and other central nervous system tumors (eg. tumors of the meninges, brain, spinal cord, cranial nerves and other parts of central nervous system, e.g. glioblastomas or medulla blastomas); head and/or neck cancer; breast tumors; circulatory system tumors (e.g. heart, mediastinum and pleura, and other intrathoracic organs, vascular tumors and tumor-associated vascular tissue); excretory system tumors (e.g. kidney, renal pelvis, ureter, bladder, other and unspecified urinary organs); gastrointestinal tract tumors (e.g. oesophagus, stomach, small intestine, colon, colorectal, rectosigmoid junction, rectum, anus and anal canal), tumors involving the liver and intrahepatic bile ducts, gall bladder, other and unspecified parts of biliary tract, pancreas, other and digestive organs); oral cavity (lip, tongue, gum, floor of mouth, palate, and other parts of mouth, parotid gland, and other parts of the salivary glands, tonsil, oropharynx, nasopharynx, pyriform sinus, hypopharynx, and other sites in the lip, oral cavity and pharynx); reproductive system tumors (e.g. vulva, vagina, Cervix uteri, Corpus uteri, uterus, ovary, and other sites associated with female genital organs, placenta, penis, prostate, testis, and other sites associated with male genital organs); respiratory tract tumors (e.g. nasal cavity and middle ear, accessory sinuses, larynx, trachea, bronchus and lung, e.g. small cell lung cancer or non-small cell lung cancer); skeletal system tumors (e.g. bone and articular cartilage of limbs, bone articular cartilage and other sites); skin tumors (e.g. malignant melanoma of the skin, non-melanoma skin cancer, basal cell carcinoma of skin, squamous cell carcinoma of skin, mesothelioma, Kaposi's sarcoma); and tumors involving other tissues incluing peripheral nerves and autonomic nervous system, connective and soft tissue, retroperitoneum and peritoneum, eye and adnexa, thyroid, adrenal gland and other endocrine glands and related structures, secondary and unspecified malignant neoplasm of lymph nodes, secondary malignant neoplasm of respiratory and digestive systems and secondary malignant neoplasm of other sites. Where hereinbef ore and subsequently a tumor, a tumor disease, a carcinoma or a cancer is mentioned, also metastasis in the original organ or tissue and/or In any other location are implied alternatively or in addition, whatever the location of the tumor and/or metastasis Is.

-11 When the S1P receptor agonist is a compound of formula 1, e.g. Compound A, or a compound of formula IVa or IVb, in one embodiment it is used in the treatment methods 1.1, 1.2, 1.3 or 1.4 for a solid tumor other than breast, prostate, bladder, kidney or lung tumor. In a series of further specific or alternative embodiments, the present Invention also provides 1.8 A method for enhancing the activity of a chemotherapeutic agent or for overcoming resistance to a chemotherapeutic agent in a subject In need thereof, comprising administering to said subject a therapeutically effective amount of a S1 P receptor agonist , e.g. a S1P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof, either concomitantly or sequentially with said chemotherapeutic agent. 1.9 A method according to 1.8 wherein the chemotherapeutic agent is an inhibitor of signal transduction pathways directed either against host cells or processes involved in tumor formation and/or metastases formation or utilised by tumour cells for proliferation, survival, differentiation or development of drug resistance. 1.10 A method as indicated above, wherein the S1P receptor agonist is administered intermittently. In a series of further specific or alternative embodiments, the present invention also provides: 2.1 A Si P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof, for use in any method as defined under 1.1 to 1.4 above, preferably for a solid tumor other than breast, prostate, bladder, kidney or lung when the S1P receptor agonist is a compound of formula I, e.g. Compound A, or a compound of formula Va or lVb. 2.2 A S1 P receptor agonist, e.g. a SIP receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof, for use in any method as defined under 1.5 to 1.10 above or 7 below. 3.1 A S1P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof, for use in the preparation of a pharmaceutical composition for use in any method as defined under 1.1 to 1.4 above, preferably for a solid tumor other than breast, prostate, bladder, kidney or lung when the S1P receptor agonist is a compound of formula 1, e.g. Compound A, or a compound of formula IVa or lVb. 3.2 A S1P receptor agonist, e.g. a S1 P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof, for use in the preparation of a pharmaceutical composition for use in any method as defined under 1.5 to 1.10 above or 7 below.

- 12 4.1 A pharmaceutical composition for use in any method as defined under 1.1 to 1.4 above comprising a S1 P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof, together with one or more pharmaceutically acceptable diluents or carriers therefor, preferably for a solid tumor other than breast, prostate, bladder, kidney or lung when the SIP receptor agonist is a compound of formula 1, e.g. Compound A, or a compound of formula IVa or IVb. 4.2 A pharmaceutical composition for use in any method as defined under 1.5 to 1.10 above or 7 below comprising a S1 P receptor agonist, e.g. a S1P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof, together with one or more pharmaceutically acceptable diluents or carriers therefor. 5.1 A pharmaceutical combination comprising a) a first agent which is a S1 P receptor agonist , e.g. a Si P receptor agonist comprising a group of formula X or a pharmaceutically acceptable salt thereof and b) a co-agent which is a chemotherapeutic agent, e.g. as defined hereinafter. 5.2 A pharmaceutical combination comprising an amount of a) a first agent which is a SiP receptor agonist , e.g. a S1 P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof, and b) a co-agent which is a chemotherapeutic agent selected from the compounds defined under section xi) below, to produce a synergistic therapeutic effect. 6. A method as defined above comprising co-administration, e.g. concomitantly or in sequence, of a therapeutically effective amount of a S1P receptor agonist, e.g. a S1P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof, and a second drug substance, said second drug substance being a chemotherapeutic agent, e.g. as indicated hereinafter. 7. A method for treating lymphoproliferative or myeloproliferative disorders, e.g. for treating tumor invasiveness or symptoms associated with such tumor growth in a subject in need thereof, comprising co-administering to said subject, e.g. concomitantly or In sequence, of a S1 P receptor agonist, e.g. a S1P receptor agonist comprising a group of formula X, or a pharmaceutically acceptable salt thereof, and a second drug substance, said second drug substance being a chemotherapeutic agent, e.g. as indicated hereinafter. By "lymphatic cancer" are meant e.g. tumors of blood and lymphatic system (e.g. Hodgkin's disease, Non-Hodgkin's lymphoma, Burkitt's lymphoma, AIDS-related lymphomas, malignant immunoproliferative diseases, multiple myeloma and malignant plasma cell neoplasms, lymphold leukemia, acute or chronic myeloid leukemia, acute or chronic lymphocytic leukemia, monocytic leukemia, other leukemias of specified cell type, leukemia of -13 unspecified cell type, other and unspecified malignant neoplasms of lymphoid, haematopoletic and related tissues, for example diffuse large cell lymphoma, T-cell lymphoma or cutaneous T-cell lymphoma). Myeloid cancer includes e.g. acute or chronic myeloid leukaemia. By the term "chemotherapeutic agent" Is meant especially any chemotherapeutic agent other than the SIP receptor agonist. It includes but is not limited to, I. an aromatase inhibitor, ii. an antiestrogen, an anti-androgen (especially in the case of prostate cancer) or a gonadorelin agonist, iii. a topoisomerase I inhibitor or a topoisomerase i inhibitor, iv. a microtubule active agent, an alkylating agent, an antineoplastic antimetabolite or a platin compound, v. a compound targeting/decreasing a protein or lipid kinase activity or a protein or lipid phosphatase activity, a further anti-anglogenic compound or a compound which induces cell differentiation processes, vi. a bradykinin 1 receptor or an angiotensin 11 antagonist, vii. a cyclooxygenase inhibitor, a bisphosphonate, a histone deacetylase inhibitor, a heparanase inhibitor (prevents heparan sulphate degradation), e.g. PI-88, a biological response modifier, preferably a lymphokine or interferons, e.g. interferon y, an ubiquitination inhibitor, or an inhibitor which blocks anti-apoptotic pathways, viii. an inhibitor of Ras oncogenic isoforms, e.g. H-Ras, K-Ras or N-Ras, or a farnesyl transferase inhibitor, e.g. L-744,832 or DK8G557, ix. a telomerase inhibitor, e.g. telomestatin, x. a protease inhibitor, a matrix metalloproteinase inhibitor, a methionine aminopeptidase inhibitor, e.g. bengamide or a derivative thereof, or a proteosome inhibitor, e.g. PS-341, and/or xi) a mTOR inhibitor. The term "aromatase inhibitor" as used herein relates to a compound which inhibits the estrogen production, i.e. the conversion of the substrates androstenedione and testosterone to estrone and estradiol, respectively. The term includes, but is not limited to steroids, especially atamestane, exemestane and formestane and, in particular, non-steroids, especially aminoglutethimide, roglethimide, pyridoglutethimide, trilostane, testolactone, ketokonazole, vorozole, fadrozole, anastrozole and letrozole. Exemestane can be administered, e.g., in the form as it Is marketed, e.g. under the trademark AROMASINTI". Formestane can be administered, e.g., in the form as It is marketed, e.g. under the - 14 trademark LENTARONm. Fadrozole can be administered, e.g., in the form as it is marketed, e.g. under the trademark AFEMATm. Anastrozole can be administered, e.g., in the form as it is marketed, e.g. under the trademark ARIMIDEXTm. Letrozole can be administered, e.g., in the form as it is marketed, e.g. under the trademark FEMARAm or FEMARTm Aminoglutethimide can be administered, e.g., in the form as it is marketed, e.g. under the trademark ORIMETENm. A combination of the invention comprising a chemotherapeutic agent which is an aromatase inhibitor is particularly useful for the treatment of hormone receptor positive tumors, e.g. breast tumors. The term "antiestrogen" as used herein relates to a compound which antagonizes the effect of estrogens at the estrogen receptor level. The term includes, but is not limited to tamoxif en, fulvestrant, raloxifene and raloxifene hydrochloride. Tamoxifen can be administered, e.g., in the form as it is marketed, e.g. under the trademark NOLVADEXTM. Raloxifene hydrochloride can be administered, e.g., in the form as it is marketed, e.g. under the trademark EVISTATm. Fulvestrant can be formulated as disclosed in US 4,659,516 or it can be administered, e.g., in the form as it is marketed, e.g. under the trademark FASLODEXTm. A combination of the invention comprising a chemotherapeutic agent which is an antiestrogen is particularly useful for the treatment of estrogen receptor positive tumors, e.g. breast tumors. The term "anti-androgen" as used herein relates to any substance which is capable of inhibiting the biological effects of androgenic hormones and includes, but is not limited to, bicalutamide (CASODEXTw), which can be formulated, e.g. as disclosed in US 4,636,505. The term "gonadorelin agonist" as used herein includes, but is not limited to abarelix, goserelin and goserelln acetate. Goserelin is disclosed in US 4,100,274 and can be administered, e.g., in the form as it Is marketed, e.g. under the trademark ZOLADEXTM. Abarelix can be formulated, eg. as disclosed in US 5,843,901. The term "topoisomerase I inhibitor" as used herein includes, but is not limited to topotecan, irinotecan, 9-nitrocamptothecin and the macromolecular camptothecin conjugate

PNU

166148 (compound Al in W099/17804). Irinotecan can be administered, e.g. in the form as it is marketed, e.g. under the trademark CAMPTOSARTm. Topotecan can be administered, e.g., in the form as it is marketed, e.g. under the trademark HYCAMTINTM. The term "topoisomerase 11 inhibitor" as used herein includes, but is not limited to the anthracyclines such as doxorubicin (including liposomal formulation, e.g. CAELYXTm), daunorubicin, epirubicin, idarubicin and nemorubicin, the anthraquinones mitoxantrorie and losoxantrone, and the podophillotoxines etoposide and teniposide. Etoposide can be - 15 administered, e.g. in the form as It Is marketed, e.g. under the trademark ETOPOPHOSh. Teniposide can be administered, e.g. in the form as it is marketed, e.g. under the trademark VM 26-BRISTOL h Doxorubicin can be administered, e.g. in the form as It Is marketed, e.g. under the trademark ADRIBLASTINm. Epirubicin can be administered, e.g. in the form as it is marketed, e.g. under the trademark FARMORUBICINm. Idarubicin can be administered, e.g. in the form as it is marketed, e.g. under the trademark ZAVEDOSM. Mitoxantrone can be administered, e.g. in the form as it is marketed, e.g. under the trademark NOVANTRONTm. The term "microtubule active agent" relates to microtubule stabilizing and microtubule destabilizing agents including, but not limited to taxanes, e.g. paclitaxel and docetaxel, vinca alkaloids, e.g., vinblastine, especially vinblastine sulfate, vincristine especially vincristine sulfate, and vinorelbine, discodermolides and epothilones and derivatives thereof, e.g. epothilone B or a derivative thereof. Paclitaxel may be administered e.g. In the form as it is marketed, e.g. TAXOL"m. Docetaxel can be administered, e.g., in the form as It is marketed, e.g. under the trademark TAXOTERETm. Vinblastine sulfate can be administered, e.g., in the form as it is marketed, e.g. under the trademark VINBLASTIN R.P.Tm. Vincristine sulfate can be administered, e.g., in the form as it is marketed, e.g. under the trademark

FARMISTIN

T

M. Discodermolide can be obtained, e.g., as disclosed in US 5,010,099. The term "alkylating agent" as used herein includes, but is not limited to busulfan, chlorambucil, cyclophosphamide, ifosfamide, melphalan or nitrosourea (BCNU or Gliadel

TM

). Cyclophosphamide can be administered, e.g., in the form as it is marketed, e.g. under the trademark CYCLOSTINTm. Ifosfamide can be administered, e.g., in the form as it is marketed, e.g. under the trademark HOLOXAN"T. The term "antineoplastic antimetabolite" includes, but is not limited to 5-fluorouracil, capecitabine, gemcitabine, cytarabine, fludarabine, thioguanine, methotrexate and edatrexate. Capecitabine can be administered, e.g., in the form as it is marketed, e.g. under the trademark XELODATm. Gemcitabine can be administered, e.g., in the form as it is marketed, e.g. under the trademark GEMZARTM. The term "platin compound" as used herein Includes, but is not limited to carboplatin, cis platin and oxaliplatin. Carboplatin can be administered, e.g., in the form as it is marketed, e.g. under the trademark CARBOPLATTm. Oxaliplatin can be administered, e.g., in the form as it is marketed, e.g. under the trademark ELOXATINTm. The term "compounds targeting/decreasing a protein or lipid kinase activity or further anti angiogenic compounds" as used herein includes, but is not limited to protein tyrosine kinase - 16 and/or serine and/or threonine kinase inhibitors or lipid kinase inhibitors, e.g. compounds targeting, decreasing or inhibiting the activity of the epidermal growth factor family of receptor tyrosine kinases (EGFR, ErbB2, ErbB3, ErbB4 as homo- or heterodimers), the vascular endothelial growth factor family of receptor tyrosine kinases (VEGFR), the platelet derived growth factor-receptors (PDGFR), the fibroblast growth factor-receptors (FGFR), the insulin-like growth factor receptor 1 (IGF-1 R), the Trk receptor tyrosine kinase family, the Axi receptor tyrosine kinase family, the Ret receptor tyrosine kinase, the Kit/SCFR receptor tyrosine kinase, members of the c-AbI family and their gene-fusion products (e.g. BCR-Abl), members of the protein kinase C (PKC) and Raf family of serine/threonine kinases, members of the MEK, SRC, JAK, FAK, PDK or Pi(3) kinase family, or of the PI(3)-kinase related kinase family, and/or members of the cyclin-dependent kinase family (CDK) and anti angiogenic compounds having another mechanism for their activity, e.g. unrelated to protein or lipid kinase inhibition. Compounds which target, decrease or inhibit the activity of VEGFR are especially compounds, proteins or antibodies which Inhibit the VEGF receptor tyrosine kinase, inhibit a VEGF receptor or bind to VEGF, and are in particular those compounds, proteins or monoclonal antibodies generically and specifically disclosed in WO 98/35958, e.g. 1-(4 chloroanilino)-4-(4-pyridylmethyl)phthalazine or a pharmaceutically acceptable salt thereof, e.g. the succinate, in WO 00/27820, e.g. a N-aryl(thio) anthranilic acid amide derivative e.g. 2-[(4-pyridyl)methylamino-N-[3-methoxy-5-(trifluoromethyl)phenyl]benzamide or 2-[(1 -oxido 4-pyridyl)methyl]amino-N-[3-trifluoromethylphenyl]benzamide, or in WO 00/09495, WO 00/59509, WO 98/11223, WO 00/27819 and EP 0 769 947; those as described by M. Prewett et al in Cancer Research 59 (1999) 5209-5218, by F. Yuan et al in Proc. Nati. Acad. Sci. USA, vol. 93, pp. 14765-14770, Dec. 1996, by Z. Zhu et al in Cancer Res. 58, 1998, 3209-3214, and by J. Mordenti et al in Toxicologic Pathology, Vol. 27, no. 1, pp 14-21, 1999; in WO 00/37502 and WO 94/10202; Angiostatirm, described by M. S. O'Reilly et al, Cell 79, 1994, 315-328; Endostatinm, described by M. S. O'Reilly et al, Cell 88,1997, 277-285; anthranilic acid amides; ZD4190; ZD6474; SU5416; SU6668; or anti-VEGF antibodies or anti-VEGF receptor antibodies,e.g. RhuMab. By antibody is meant intact monoclonal antibodies, polyclonal antibodies, multispecif ic antibodies formed from at least 2 intact antibodies, and antibodies fragments so long as they exhibit the desired biological activity. Compounds which target, decrease or inhibit the activity of the epidermal growth factor receptor family are especially compounds, proteins or antibodies which inhibit members of the EGF receptor tyrosine kinase family, e.g. EGF receptor, ErbB2, ErbB3 and ErbB4 or bind - 17 to EGF or EGF related ligands, or which have a dual inhibiting effect on the ErbB and VEGF receptor kinase and are in particular those compounds, proteins or monoclonal antibodies generically and specifically disclosed in WO 97/02266, e.g. the compound of ex. 39, or in EP 0 564 409, WO 99/03854, EP 0520722, EP 0 566 226, EP 0 787 722, EP 0 837 063, US 5,747,498, WO 98/10767, WO 97/30034, WO 97/49688, WO 97/38983 and, especially, WO 96/30347 (e.g. compound known as CP 358774), WO 96/33980 (e.g. compound ZD 1839) and WO 95/03283 (e.g. compound ZM1 05180) or PCT/EP02/08780; e.g. trastuzumab (Herpetin"), cetuximab, Iressa, OSI-774, Cl-1033, EKB-569, GW-2016, E1.1, E2.4, E2.5, E6.2, E6.4, E2.1 1, E6.3 or E7.6.3. Compounds which target, decrease or inhibit the activity of PDGFR are especially compounds which inhibit the PDGF receptor, e.g. a N-phenyl-2-pyrimidine-amine derivative, e.g. imatinib. Compounds which target, decrease or inhibit the activity of c-AbI family members and their gene fusion products, e.g. a N-phenyl-2-pyrimidine-amine derivative, e.g. imatinib; PD180970; AG957; or NSC 680410. Compounds which target, decrease or inhibit the activity of protein kinase C, Raf, MEK, SRC, JAK, FAK and PDK family members, or PI(3) kinase or PI(3) kinase-related family members, and/or members of the cyclin-dependent kinase family (CDK) are especially those staurosporine derivatives disclosed in EP 0 296 110, e.g. midostaurin; examples of further compounds include e.g. UCN-01, safingol, BAY 43-9006, Bryostatin 1, Perifosine; U0126; Ilmofosine; RO 318220 and RO 320432; GO 6976; Isis 3521; or LY333531/LY3791 9 6 . Further anti-angiogenic compounds are e.g. thalidomide (THALOMID) and TNP-470. Compounds which target, decrease or inhibit the activity of a protein or lipid phosphatase are e.g. inhibitors of phosphatase 1, phosphatase 2A, PTEN or CDC25, e.g. okadaic acid or a derivative thereof. Compounds which induce cell differentiation processes are e.g. retinoic acid, a-, y-- or S tocopherol or a-, -y- or 8-tocotrienol. The term cyclooxygenase inhibitor as used herein Includes, but is not limited to, e.g. celecoxib (CelebrexR), rofecoxib (Vioxx"), etoricoxib, valdecoxib or a 5-alkyl-2 arylaminophenylacetic acid, e.g. 5-methyi-2-(2'-chloro-6'-fluoroanilino)phenyI acetic acid. The term "histone deacetylase inhibitor" as used herein includes, but is not limited to MS-27 275, SAHA, pyroxamide, FR-901228 or valproic acid.

- 18 The term "bisphosphonates" as used herein Includes, but is not limited to, etridonic, clodronic, tiludronic, pamidronic, alendronic, lbandronic, risedronic and zoledronic acid. "Etridonic acid" can be administered, e.g., in the form as it is marketed, e.g. under the trademark DIDRONELIA. "Clodronic acid" can be administered, e.g., in the form as it is marketed, e.g. under the trademark BONEFOSm. "Tiludronic acid" can be administered, e.g., in the form as it is marketed, e.g. under the trademark SKELID"". "Pamidronic acid" can be administered, e.g. in the form as it is marketed, e.g. under the trademark AREDIATf. "Alendronic acid" can be administered, e.g., in the form as it is marketed, e.g. under the trademark FOSAMAX~m. "Ibandronic acid" can be administered, e.g., In the form as it is marketed, e.g. under the trademark BONDRANATTm. "Risedronic acid" can be administered, e.g., in the form as it is marketed, e.g. under the trademark ACTONELT". "Zoledronic acid" can be administered, e.g. in the form as It is marketed, e.g. under the trademark ZOMETATh The term "matrix metalloproteinase inhibitor" as used herein includes, but Is not limited to collagen peptidomimetic and nonpetidonimetic inhibitors, tetracycline derivatives, e.g. hydroxamate peptidomimetic inhibitor batimastat and its orally bioavailable analogue marimastat, prinomastat, BMS-279251, BAY 12-9566, TAA211 or AAJ996. The term "mTOR inhibitor" as used herein includes, but is not limited to rapamycin (sirolimus) or a derivative thereof. Rapamycin is a known macrolide antibiotic produced by Streptomyces hygroscopicus. Suitable derivatives of rapamycin include e.g. compounds of formula A 41 90 11 18 29 2 2 8 27 A 13 15 is 2 1 22 wherein

R

1 . is CH 3 or C 34 alkynyl, R2. is H or -CH 2

-CH

2 -OH, 3-hydroxy-2-(hydroxymethyl)-2-methy-propanoyI or tetrazolyl, and X. is =0, (H,H) or (H,0H) provided that R2. is other than H when X. is =0 and R 1 ,. Is CH 3

.

-19 or a prodrug thereof when R2 is -CH 2

-CH

2 OH, e.g. a physiologically hydrolysable ether thereof. Compounds of formula A are disclosed e.g. In WO 94/09010, WO 95/16691, WO 96/41807, USP 5,362,718 or WO 99/15530 which are incorporated herein by reference. They may be prepared as diciosed or by analogy to the procedures described in these references Preferred rapamycin derivatives are 32-deoxorapamycin, 16-pent-2-ynyloxy-3 2 deoxorapamycin, 16-pent-2-ynyloxy-32(S)-dihydro-rapamycin, 16-pent-2-ynyloxy-32(S) dihydro-40-0-(2-hydroxyethyl)-rapamycin and, more preferably, 40-0-(2-hydroxyethyl)-rapamycin. Further examples of rapamycin derivatives include e.g. CC1779 or 40- [ 3 -hydroxy.2-(hydroxymethyl)-2-methylpropanoate]-rapamycin or a pharmaceutically acceptable salt thereof, as disclosed in USP 5,362,718, ABT578 or 40 (tetrazolyl)-rapamycin, particularly 40-epi-(tetrazolyl)-rapamycin, e.g. as disclosed in WO 99/15530, or rapalogs as disclosed e.g. in WO 98/02441 and WO01/14387, e.g. AP23573. In each case where citations of patent applications or scientific publications are given, the subject-matter relating to the compounds is hereby incorporated Into the present application by reference. Comprised are likewise the pharmaceutically acceptable salts thereof, the corresponding racemates, diastereoisomers, enantiomers, tautomers as well as the corresponding crystal modifications of above disclosed compounds where present, e.g. solvates, hydrates and polymorphs, which are disclosed therein. The compounds used as active ingredients in the combinations of the invention can be prepared and administered as described in the cited documents, respectively. Also within the scope of this invention is the combination of more than two separate active ingredients as set forth above, i.e. a pharmaceutical combination within the scope of this invention could include three active ingredients or more. Further both the first agent and the co-agent are not the identical ingredient. Utility of the S1 P agonists, e.g. the S1 P agonists comprising a group of formula X, in treating solid tumors as hereinabove specified, may be demonstrated in animal test methods as well as in clinic, for example in accordance with the methods hereinafter described. A. In Vitro A.1 Antitumor Activity A mouse breast cancer cell line originally isolated from mammary carcinomas is used, e.g. JygMC(A). The cell number is adjusted to 5x10' for plating in fresh medium before the procedure. Cells are incubated with fresh medium containing 2.5mM of thymidine without FCS for 12 h and then washed twice with PBS, followed by addition of - 20 fresh medium with 10% FCS and additionally incubated for another 12h. Thereafter the cells are incubated with fresh medium containing 2.5mM of thymidine without FCS for 12h. To release the cells from the block, the cells are washed twice with PBS and replated in fresh medium with 10% FCS. After synchronisation, the cells are incubated with or without various concentrations of a compound of formula I for 3, 6, 9, 12, 18 or 24h. The cells are harvested after treatment with 0.2% EDTA, fixed with ice-cold 70% ethanol solution, hydrolyzed with 250pg/mI of RNaseA (type 1 -A: Sigma Chem. Co.) at 37*C for 30 mn and stained with propidlum iodide at 10mg/ml for 20 mn. After the Incubation period, the number of cells is determined both by counting cells in a Coulter counter and by the SRB colorimetric assay. Under these conditions an S1P agonist, e.g. Compound B in hydrochloride salt form, inhibits the proliferation of the tumor cells at concentrations ranging from 10-12 to 10'6 M. A.2 SiP-Mediated HUVEC Tube Formation Assay For the tube formation assay, HUVEC from passage 2-8 are used and are never greater than 70% confluent before harvesting. Cells are prepared for the assay by washing with Herpes Balanced Saline Solution (HBSS from Clonetics) and then trypsinizing with Trypsin/EDTA (0.25 mg/mI, from Clonetics). After approximately 90 % of the cells have lifted off the plate, an equal volume of Trypsin Neutralizing Solution (TNS from Clonetics) is added and the cells are collected into a conical tube containing at least 10 mi of EBM-2 (Clonetics) + 0.1 % BSA (Sigma) media. Cells are centrifuged at 1000 rpm for 5 minutes and the supernatant is removed and replaced with 5 mi of fresh EBM-2 + 0.1 % BSA. Cells are counted using a hemacytometer and the volume of the cell suspension is adjusted to achieve a concentration of 500,000 cells/mi. Conical tubes are prepared with test compounds at 100 nM, and pertussin toxin (PTx) at 10 ng/ml in each, then 1 mi of the cell suspension is added to each tube. Tubes are then Incubated for '/ hour at 37 QC, 5 % C02. The migration assay is performed using Fluoro-Blok 24-Muftiwell Insert Plates coated with fibronectin (8 pm pore size, Falcon #351147) instead of the individual inserts in a 24-well plate. Cells and test compounds are prepared and pre-incubated as described above, then 100 pi is added to each approriate well in the Insert Plate. 300 pl of the EBM-2 + 2 % charcoal-stripped media without S1 P is added to the bottoms of the wells marked for no stimulation (-), and 300 p of the media containing S1P (500 nM) is added to the bottoms of the wells marked for stimulation (+). The plate is then incubated for 4 hours at 37 RC, 5 % CO. Calcein AM, 50 pg/vial, (Molecular Probes #C3100) is prepared by first adding 20 p1 DMSO to the vial. Then 12.5 ml of HBSS (per plate) is warmed to 37 gC and 150 pl is added to the -21 vial. The contents of the vial are then transferred back to the remaining HBSS to make the final concentration 4 pg/ml Calcein AM. The Fluoro-Blok plate Is removed from the incubator and the top Insert plate is separated and "flicked" to remove excess media clinging to the inserts. The insert plate is then transferred to a fresh 24-well plate containing 500 pl/well of the 4 pg/ml Calcein AM. The plate is then Incubated for 1 1 hours at 37 OC, 5 % CO 2 . After incubation, the plate Is read on a Cytofluor 11 at an excitation of 485 nm and emission of 530 nm. The Fluoro-Blok coating in the inserts allows only the cells that have migrated to the bottom to be counted. Data are transferred to Excel for calculations, graphs are created using SigmaPlot, and SigmaStat is used for significance tests (t-test). (Figure 7). Tube formation is quantitated by counting the number of branching points (two Independent cords connecting) in 3 independent fields at 4x magnification. The results are reported as follows: Treatment Branching Points PBS 8±5 Sip 42t 13 FTY720-Phosphate 48 * 15 FTY720-Phosphate + S1 P 14 t 7 Compound C-Phosphate 44 * 16 Compound C-Phosphate + S1P 18 t 6 These results demonstrate the unique ability of FTY720-Phosphate or Compound C Phosphate to act as an agonist of anglogenesis on Its own, but then surprisingly, as an antagonist of Si P-mediated angiogenesis. Compound C-Phosphate is preferably the racemate or the R-enantiomer. PTx is used as a control to inhibit Gia (EDG-1) mediated activity. B. In Vivo B.1 Antitumor Activity Antitumor activity is expressed as T/C% (mean Increase In tumor volumes of treated animals divided by the mean increase of tumor volumes of control animals multiplied by 100).

- 22 Aliquots of cancer cells (1x ,07), e.g. human A375 melanoma cells, are transplanted into BALB/c-nulnu mice. When the tumors have reached ca. 1 Oxi 0 mm in size, the animals are assigned randomly to four subgroups and the treatment with a compound of formula I is initiated. Animals are sacrificed after 2 week treatment, at which times tumors and tissues are harvested and prepared for morphological and molecular analysis. The size of the tumors is determined with a caliper. In this assay, an S1P agonist, e.g. Compound B or C (in the hydrochloride salt form), slows tumor growth when administered at a dose of from 0.5 to 5 mg/kg vs saline control: for example, Compound C-HCI when administered at a dose of 2.5mg/kg 5x/week results in a final T/C value of 30%. B.2 Combination with a VEGF-R protein tyrosin kinase inhibitor Nude mice transplanted with human MDA-MB-435 breast tumors are treated for 2 weeks with a VEGF-R protein tyrosin kinase inhibitor, e.g. 1-(4-chloroanilino)-4-( 4 pyridylmethyl)phthaiazine succinate, at a dose of 100 mg/kg p.o. 5x/week, a SIP receptor agonist, e.g. Compound C (hydrochloride salt), at a dose of 2.5 mg/kg i.v. 5x/week, or a combination of both. Antitumor is expressed as T/C% as indicated above. A combination of Compound C-HCI with 1-(4-chloroanilino)-4-(4-pyridylmethyl)phthalazine succinate produces a greater antitumor effect (T/C% 27) as compared to either agent alone (Compound C-HCI, T/C 66%; 1-(4-chioroanilino)-4-(4-pyridylmethyl)phthalazine succinate, T/C% 91). Good antitumor responses are also obtained when nude mice are transplanted with human A375 melanoma cells and treated in a similar way with the same combination: the combined treatment results in a T/C% 15 whereas treatment with each agent alone results in a T/C% 35 and 44, respectively. B.3 Antiangiogenic Activity Porous chambers containing (i) sphingosine-1 -phosphate (5 p M/chamber) or (ii) human VEGF (1 pg/chamber) in 0.5 ml of 0.8% w/v agar (containing heparin, 20 U/lm) are implanted subcutaneously in the flank of mice. S1P or VEGF induces the growth of vascularized tissue around the chamber. This response is dose-dependent and can be quantified by measuring the weight and blood content of the tissue. Mice are treated once a day (i) orally with Compound A (0.3, 3, 30 or 50 mg/kg) or (ii) intravenously with the R enantiomer of Compound C (2.5 mg/kg) or (ii) intravenously with the S enantiomer of Compound C (2.5 mg/kg) or (iv) orally or intravenously with vehicle (5% glucose, 10 mVkg), starting 4-6 hours before implantation of the chambers and continuing for 4 days. The animals are sacrificed for measurement of the vascularized tissues 24 h after the last dose. The weight and blood content of the vascularized tissues around the chamber is determined.

- 23 Animals treated with Compound A or with the R or S enantiomer of Compound C show reduced weight and/or blood content of the vascularized tissues compared to animals treated with vehicle alone. C. Clinical Trial C.i Investigation of clinical benefit of a S1P receptor agonist, e.g. a compound of formula I, I or l1l, e.g. Compound A, B or C 20 patients with progressing, advanced-stage solid tumors, resistant or refractory to standard therapies, to receive said compound at a dosage as determined by a dose escalating study. The general clinical state of the patient is Investigated weekly by physical and laboratory examination. Changes in tumor and metastases burden are assessed every 2 months by radiological examination. Initially patients receive treatment for 2 months. Thereafter, they remain on treatment for as long as their disease does not progress and the drug is satisfactorily tolerated. Main variables for evaluation: Safety (adverse events), standard serum biochemistry and haematology, tumor dimensions by computerised tomographic (CT) scan or magnetic resonance imaging (MRI). C.2 Combined Treatment Suitable clinical studies are, for example, open label non-randomized, dose escalation studies in patients with advanced solid tumors. Such studies prove in particular the synergism of the active ingredients of the combination of the invention. The beneficial effects on proliferative diseases can be determined directly through the results of these studies or by changes in the study design which are known as such to a person skilled in the art. Such studies are, in particular, suitable to compare the effects of a monotherapy using the active ingredients and a combination of the invention. Preferably, the dose of agent (a) is escalated until the Maximum Tolerated Dosage is reached, and the co-agent (b) is administered with a fixed dose. Alternatively, the agent (a) is administered in a fixed dose and the dose of co-agent (b) is escalated. Each patient receives doses of the agent (a) either daily or intermittent. The efficacy of the treatment can be determined in such studies, e.g., after 12, 18 or 24 weeks by radiologic evaluation of the tumors every 6 weeks. Alternatively, a placebo-controlled, double blind study can be used in order to prove the benefits of the combination of the invention mentioned herein. Daily dosages required in practicing the method of the present invention when a S1 P receptor agonist alone is used will vary depending upon, for example, the compound used, the host, the mode of administration and the severity of the condition to be treated. A preferred daily dosage range is about from 0.1 to 100 mg as a single dose or in divided - 24 doses. Suitable daily dosages for patients are on the order of from e.g. 0.1 to 50 mg p.o. The Si P receptor agonist may be administered by any conventional route, in particular enterally, e.g. orally, e.g. in the form of tablets, capsules, drink solutions, nasally, pulmonary (by inhalation) or parenterally, e.g. in the form of injectable solutions or suspensions. Suitable unit dosage forms for oral administration comprise from ca. 0.1 to 30 mg, usually 0.25 to 30 mg S1 P receptor agonist, together with one or more pharmaceutically acceptable diluents or carriers therefore. In order to inhibit angiogenesis it is important to select a sufficiently high dose of the S1P receptor agonist, as low concentrations of SIP receptor agonists promote angiogenesis. A suitable dose for providing an anti-angiogenic effect when a S1 P agonist is administered to a patient may be selected by concentration- and dose-escalating studies as described at A, B, and C above. The combination of the invention can also be applied in combination with surgical intervention, mild prolonged whole body hyperthermia and/or irradiation therapy. The administration of a pharmaceutical combination of the invention results in a beneficial effect, e.g. a synergistic therapeutic effect, e.g. with regard to slowing down, arresting or reversing the neoplasm formation,r metastases spread or growth or a longer duration of tumor response or inhibition of angiogenesis; it may also result in other beneficial effects, e.g. less side-effects, an improved quality of life or a decreased mortality and morbidity, compared to a monotherapy applying only one of the pharmaceutically active ingredients used in the combination of the invention, in particular in the treatment of a tumor that is refractory to other chemotherapeutics known as anti-cancer agents. A further benefit is that lower doses of the active ingredients of the combination of the invention can be used, for example, that the dosages need not only often be smaller but are also applied less frequently, or can be used in order to diminish the incidence of side-effects, while controlling the growth of neoplasm formation. This is in accordance with the desires and requirements of the patients to be treated. According to one embodiment of the invention, a preferred pharmaceutical combination comprises a) a compound of formula 1, 11, 111, Va, lVb, V or VI, e.g. Compound A, B or C, and b) as a co-agent, one or more compounds as indicated in paragraphs (ii), (iii), (iv), (v), (vii) or (xi) above, e.g. carboplatin, cisplatinum, paclitaxel, docetaxel, gemcitabine, doxorubicin, a compound targeting, decreasing or inhibiting the activity of the vascular endothelial growth factor family of receptor tyrosine kinases (VEGFR) or the platelet-derived growth factor receptors (PDGFR), a bisphosphonate or a mTOR inhibitor.

- 25 A further embodiment of the invention relates to the use of S1 P receptor agonist (a) in combination with a chemotherapeutic agent (b) In the treatment of a lymphatic or myeioid cancer, e.g. as disclosed above. The combination may comprise as a further co-agent b) e.g. busulfan, cytarabine, 6-thioguanine, fludarabine, hydroxyurea, procarbazine, bleomycin or methotrexate. Topolsomerase i inhibitors e,g. daunorubicin or, particularly, compounds which target, decrease or inhibit the activity of PDGFR or of c-AbI family members and their gene fusion products, e.g. imatinib, are preferred as co-agent (b), e.g. for use in the treatment of a lymphatic cancer. The terms "co-administration" or "combined administration" or the like as utilized herein are meant to encompass administration of the selected therapeutic agents to a single patient, and are intended to include treatment regimens in which the agents are not necessarily administered by the same route of administration or at the same time. It is one objective of this Invention to provide a pharmaceutical composition comprising a quantity, which is jointly therapeutically effective against a proliferative malignant disease comprising a combination of the invention. In this composition, the first agent a) and co agent (b) can be administered together, one after the other or separately in one combined unit dosage form or in two separate unit dosage forms. The unit dosage form may also be a fixed combination. The pharmaceutical compositions according to the invention can be prepared in a manner known per se and are those suitable for enteral, such as oral or rectal, and parenteral administration to mammals (warm-blooded animals), including humans, comprising a therapeutically effective amount of at least one pharmacologically active combination partner alone, e.g. as indicated above, or in combination with one or more pharmaceutically acceptable carriers or diluents, especially suitable for enteral or parenteral application. Suitable pharmaceutical compositions contain, for example, from about 0.1 % to about 99.9%, preferably from about 1 % to about 60 %, of the active ingredient(s). Pharmaceutical preparations for the combination therapy for enteral or parenteral administration are, for example, those in unit dosage forms, such as sugar-coated tablets, tablets, capsules or suppositories, or ampoules. If not indicated otherwise, these are prepared in a manner known per se, for example by means of conventional mixing, granulating, sugar-coating, dissolving or lyophilizing processes. It will be appreciated that the unit content of a combination partner contained in an individual dose of each dosage form need not in itself constitute an effective amount since the necessary effective amount can be reached by administration of a plurality of dosage units.

-26 In particular, a therapeutically effective amount of each of the combination partner of the combination of the Invention may be administered simultaneously or sequentially and In any order, and the components may be administered separately or as a fixed combination. For example, the method of delay of progression or treatment of a proliferative malignant disease according to the Invention may comprise (I) administration of the first agent a) in free or pharmaceutically acceptable salt form and (1i) administration of a co-agent b) in free or pharmaceutically acceptable salt form, simultaneously or sequentially in any order, in jointly therapeutically effective amounts, preferably in synergistically effective amounts, e.g. in daily or Intermittently dosages corresponding to the amounts described herein. The Individual combination partners of the combination of the Invention may be administered separately at different times during the course of therapy or concurrently in divided or single combination forms. Furthermore, the term administering also encompasses the use of a pro-drug of a combination partner that convert in vivo to the combination partner as such. The instant invention is therefore to be understood as embracing all such regimens of simultaneous or alternating treatment and the term "administering" is to be interpreted accordingly. The effective dosage of each of the combination partners employed in the combination of the invention may vary depending on the particular compound or pharmaceutical composition employed, the mode of administration, the condition being treated, the severity of the condition being treated. Thus, the dosage regimen of the combination of the invention is selected in accordance with a variety of factors including the route of administration and the renal and hepatic function of the patient. A physician, clinician or veterinarian of ordinary skill can readily determine and prescribe the effective amount of the single active ingredients required to prevent, counter or arrest the progress of the condition. Optimal precision in achieving concentration of the active ingredients within the range that yields efficacy without toxicity requires a regimen based on the kinetics of the active ingredients' availability to target sites. Daily dosages for the first agent or component (a) will, of course, vary depending on a variety of factors, for example the compound chosen, the particular condition to be treated and the desired effect. In general, however, satisfactory results are achieved on administration of a SIP receptor agonist, e.g. Compound A, B or C, at daily dosage rates of the order of ca. 0.1 to 100 mg as a single dose or in divided doses. The SiP receptor agonist may be administered by any conventional route, in particular enterally, e.g. orally, e.g. in the form of tablets, capsules, drink solutions or parenterally, e.g. In the form of injectable solutions or suspensions. Suitable unit dosage forms for oral administration - 27 comprise from ca. 0.1 to 30 mg component (a), e.g. 0.1 to 25 mg, together with one or more pharmaceutically acceptable diluents or carriers therefor. Fadrozole may be administered orally to a human in a dosage range varying from about 0.5 to about 10 mg/day, preferably from about 1 to about 2.5 mg/day. Exemestane may be administered orally to a human in a dosage range varying from about 5 to about 200 mg/day, preferably from about 10 to about 25 mg/day, or parenterally from about 50 to 500 mg/day, preferably from about 100 to about 250 mg/day. If the drug shall be administered in a separate pharmaceutical composition, it can be administered in the form disclosed In GB 2,177,700. Formestane may be administered parenterally to a human in a dosage range varying from about 100 to 500 mg/day, preferably from about 250 to about 300 mg/day. Anastrozole may be administered orally to a human in a dosage range varying from about 0.25 to 20 mg/day, preferably from about 0.5 to about 2.5 mg/day. Aminogluthemide may be administered to a human in a dosage range varying from about 200 to 500 mg/day. Tamoxifen citrate may be administered to a human in a dosage range varying from about 10 to 40 mg/day. Vinblastine may be administered to a human in a dosage range varying from about 1.5 to 10 mg/m 2 day. Vincristine sulfate may be administered parenterally to a human in a dosage range varying from about 0.025 to 0.05 mg/kg body weight -week. Vinorelbine may be administered to a human In a dosage range varying from about 10 to 50 mg/m 2 day. Etoposide phosphate may be administered to a human in a dosage range varying from about 25 to 115 mg/m 2 day, e.g. 56.8 or 113.6 mg/m 2 day. Teniposide may be administered to a human in a dosage range varying from about 75 to 150 mg about every two weeks. Doxorubicin may be administered to a human in a dosage range varying from about 10 to 100 mg/m 2 day, e.g. 25 or 50 mg/m 2 day. Epirubicin may be administered to a human In a dosage range varying from about 10 to 200 mg/m 2 day. Idarubicin may be administered to a human In a dosage range varying from about 0.5 to 50 mg/m 2 day. Mitoxantrone may be administered to a human in a dosage range varying from about 2.5 to 25 mg/m 2 day. Paclitaxel may be administered to a human in a dosage range varying from about 50 to 300 mg/m 2 day. Docetaxel may be administered to a human in a dosage range varying from about 25 to 100 mg/m 2 day.

-28 Cyclophosphamide may be administered to a human in a dosage range varying from about 50 to 1500 mg/m 2 day. Melphalan may be administered to a human in a dosage range varying from about 0.5 to 10 mg/m 2 day. 5-Fluorouracii may be administered to a human in a dosage range varying from about 50 to 1000 mg/m 2 day, e.g. 500 mg/m 2 day. Capecitabine may be administered to a human in a dosage range varying from about 10 to 1000 mg/m 2 day. Gemcitabine hydrochloride may be administered to a human in a dosage range varying from about 1000 mg/m 2 /week. Methotrexate may be administered to a human in a dosage range varying from about 5 to 500 mg/m 2 day. Topotecan may be administered to a human in a dosage range varying from about 1 to 5 mg/m 2 day. irinotecan may be administered to a human in a dosage range varying from about 50 to 350 mg/m 2 day. Carboplatin may be administered to a human in a dosage range varying from about 200 to 400 mg/m 2 about every four weeks. Cisplatin may be administered to a human in a dosage range varying from about 25 to 75 mg/m 2 about every three weeks. Oxaiiplatin may be administered to a human in a dosage range varying from about 50 to 85 mg/m 2 every two weeks. imatinib may be administered to a human in a dosage in the range of about 2.5 to 850 mg/day, more preferably 5 to 600 mg/day and most preferably 20 to 300 mg/day. Alendronic acid may be administered to a human in a dosage range varying from about 5 to 10 mg/day. Clodronic acid may be administered to a human e.g. in a dosage range varying from about 750 to 1500 mg/day. Etridonic acid may be administered to a human in a dosage range varying from about 200 to 400 mg/day. ibandronic acid may be administered to a human in a dosage range varying from about 1 to 4 mg every three to four weeks. Risedronic acid may be administered to a human in a dosage range varying from about 20 to 30 mg/day. Pamidronic acid may be administered to a human in a dosage range varying from about 15 to 90 mg every three to four weeks. Tiludronic acid may be administered to a human in a dosage range varying from about 200 to 400 mg/day. Trastuzumab may be administered to a human in a dosage range varying from about 1 to 4 mg/m 2 /week. Bicalutamide may be administered to a human in a dosage range varying from about 25 to 50 mg/m 2 day.

- 29 1-( 4 -chloroanilino)-4-(4-pyridylmethyl)phthalazine or salt thereof, e.g. succinate, may be administered to a human in a dosage range of about 50 to 1500, more preferably about 100 to 750, and most preferably 250 to 500, mg/day. Rapamycin or a derivative thereof, e.g. 40-0-(2-hydroxyethyI)-rapamycin, may be administered in a dosage range varying from about 0.1 to 25 mg. Formulation Example: soft capsules Compound of formula I, e.g. Compound A, HCI 30 mg Polyethylene glycol 300 300 mg Polysorbate 80 20 mg Total 350 mg The S1 P receptor agonists, e.g. a S1P receptor agonist comprising a group of formula X, are well tolerated at dosages required for use in accordance with the present invention. For example, the acute LD 50 for Compound A is > 10 mg/kg p.o. in rats and monkeys. In a further aspect, the present invention relates to the use of S1P agonists as pro angiogenic drugs. Induction of neo-angiogenesis has lately been recognized as an excellent target in a number of conditions (e.g.myocardial anglogenesis, wound healing or diabetic vascular dysfunction/vasculopathy). As described above, high concentrations of S1 P receptor agonists (2 pM or greater, e.g. 2-5 pM or around 5 pM) exhibit anti-angiogenic effects, and S1P receptor agonists can inhibit VEGF-induced angiogenesis. In contrast, low concentrations (0.1 -1 pM, e.g. 0.1 - 0.5 pM or 0.5 - 1 pM) of S1 P agonists have an enhancing effect on angiogenesis and are able to potentiate VEGF-mediated angiogenesis. Thus, S1P agonists may have biphasic effects in angiogenesis. Accordingly, the present invention further provides: 8. Use of a S1P agonist, e.g. a S1P agonist comprising a group of formula X, e.g. Compound A or Compound A-phosphate, in the Induction of the neo-anglogenesis process, - 30 e.g. as a pro-angionenic agent, e.g. in indications where a promotion of angiogenesis is indicated; 9. A process for the preparation of a medicament for the treatment or prevention of diseases mediated by the inhibition of the neo-angiogenesis process, e.g. mediated by anti angionenic factors, e.g. in indications where a promotion of angiogenesis is Indicated, e.g. in wound healing or in the treatment of myocardial infarction or diabetic vascular dysfunction/vasculopathy, comprising using a S1 P receptor agonist, e.g. a S1 P agonist comprising a group of formula X, e.g. Compound A or Compound A-phosphate, as an active ingredient; 10. A method of treating or preventing diseases mediated by the inhibition of the neo anglogenesis process, e.g. mediated by anti-angionenic factors, e.g. in indications where a promotion of angiogenesis is indicated, such as e.g. in wound healing or in the treatment of myocardial infarction or diabetic vascular dysfunction/vasculopathy, comprising administering an effective amount of a S1 P receptor agonist, e.g. a S1 P agonist comprising a group of formula X, e.g. Compound A or Compound A-phosphate, to a subject in need of such treatment. S1 P agonists suitable for promoting angiogenesis include those defined above in relation to the treatment of cancer, e.g. S1P agonists comprising a group of formula X or compounds according to formulae I to IX, or pharmaceutically acceptable salts or esters thereof. Preferably the S1P agonist is Compound A-phosphate. The S1 P agonist may be used alone, or in combination with one or more further agents which promote angiogenesis, e.g. VEGF. In order to promote angiogenesis it is important to select a sufficiently low dose of the S1P receptor agonist, as high concentrations of S1P receptor agonists inhibit anglogenesis. A suitable dose for providing a pro-angiogenic effect when a S1 P agonist is administered to a patient may be selected by concentration- and dose-escalating studies as described at A, B, and C above.

-31 Description of the Figures Figure 1 shows that Compound A-phosphate strongly promotes capillary-like network formation in a bell-shape dose-dependent manner showing maximal activity around 0.5 pM. Figure 2 shows that both Compound A-phosphate and Compound A at 0.5 - 1 pM do not attenuate VEGF-mediated remodelling but rather cooperate with polypeptide growth factor. Figure 3 Shows that Compound A-phosphate as well as S1 P-stimulated tube formation is practically completely inhibited by pertussis toxin (PTX, 50 ng/ml), an inhibitor of heterotrimeric G proteins of oc,.-type. This may be interpreted as a possible involvement of EDG-1 (S1 P 1 ) receptor-mediated signaling events in Compound A-phosphate-stimulated bioresponses. Figure 4 Shows, that sphingosine at 1 pM, which itself seems to be less potent than S1P, attenuates the ability of both S1P and Compound A-phosphate to induce capillary-like structures, without having an inhibitory effect on the VEGF-induced tube formation. In this respect, sphingosine behaves different from Compound A. The data indicate that the balance between sphingosine and S1P seems to be critically important for endothelial cell activation/angiogenesis most likely via the EDG receptor family. Importantly, high concentrations of sphingosine and Compound A (2 - 5 pM) inhibits VEGF-triggered tube formation. Figure 5 Shows that the treatment of HUVEC with Compound A-phosphate at 0.5 pM may result in transient activation of ERK1/2 with a peak of phosphorylation/activation at 10 minutes and returning to baseline by 20 minutes Figure 6 it was tested whether Compound A, Compound A-phosphate, sphingosine or S1 P also do induce tissue factor on HUVEC. The data found demonstrate that none of these compounds alone or in combinations may elevate tissue factor activity as shown in Figure 6. Compound A and Compound A-phosphate may slightly enhance the VEGF- but not TNF-a-induced tissue factor.

- 32 Figure 7 Shows the effect of Compound C In a SiP-mediated HUVEC tube formation assay. The following abbreviations are used: BSA: bovine serum albumine ECGS: endothelial cell growth factor set ECL: enhanced chemiluminescence S: sphingosine PBS: phosphate-buffered saline JNK1/2: c-jun-N-terminal kinase1/2 RT: room temperature TF equivalents: tissue factor equivalents EGR-1/NFAT: early growth response protein 1/nuclear factor of activated T-cells F1 P: Compound A-phosphate (FTY720-phosphate) Utility of the S1P receptor agonists, e.g. the S1 P agonists comprising a group of formula X, in the promotion of angiogenesis may be demonstrated for example in accordance with the methods described hereinafter. D. Cell culture and Materials Human umbilical vein endothelial cells (HUVEC) are cultured at 370C and 5% C02 in medium M1 99 supplemented with 20% SCS (HyClone, Logan, UT), 1 U/mI heparin, 50 pg/ml ECGS, 2 mM glutamine, 100 U/ml penicillin and 0.1 mg/mI streptomycin. Cells are used for experiments up to passage number 5. Short-starved HUVEC are obtained by starving with 1% SCS-containing M199 for 5 h. Recombinant human VEGF 1 6S is obtained from PromoCell (Heidelberg, Germany). Phospho-specific ERK1/2, p38 kinase polyclonal antibodies, nonphospho ERK1/2 antibodies and LumiGLO chemiluminescent reagent are from New England BioLabs (Beverly, MA), polyclonal lKB antibodies from Santa Cruz Biotechnology (Santa Cruz, Calif.). Peroxidase-conjugated donkey anti-rabbit Immunoglobulin G (IgG) and sheep anti-mouse IgG are purchased from Amersham LIFE SCIENCE (Amershan Place, England). Immobilon-P transfer membranes are products of Millipore (Bedford, MA). S is obtained from Sigma Chemical Co.; SIP is from Biomol. Compound A-phosphate stock solution is prepared by the following protocol. Compound A-phosphate is dissolved in methanol tracing with concentrated HCI (0.5 mg Compound A-phosphate in 500 pl of methanol plus 2 pl of HCI). Solvent from the resulting solution is evaporated under vacuum and the residue obtained is redissolved (variant 1) In 0.1 % of defatted BSA solution in sterile demonized water (500 pl) or (variant 2) In 0.5 % Triton X-100 in demonized water. The resulting stock solutions (2.5 mM) are sonicated and stored at 40C.

- 33 Clotting Assay Cells are seeded in 6-well plates at 80-90% confluency and grown overnight. Cells are scraped from the plates and analyzed for tissue factor activity according to the method as described in Clauss, M., J. Biol. Chem. 271,17629-17634 (1996), Mechtcheriakova, D., Blood 93,3811-3823 (1999). Briefly, after induction for 4 hours with VEGF (1.5 nM), TNF-ct (100 U/ml), S (0.5-2 pM), S1 P (0.5-2 pM), Compound A (0.5-2 pM), and Compound

A

phosphate (0.5-2 pM), cells are washed twice and then scraped in 1 ml clotting buffer (12 mM sodium acetate, 7 mM diethylbarbitate and 130 mM sodium chloride; pH 7.4). 50 pl of resuspended cells are mixed with 50 pl of citrated plasma, and clotting times are determined after recalcification with 50 pi of 20 mM CaC1 2 solution at 37 0 C. TF-equivalents are determined by using a standard curve obtained from rabbit brain thromboplastin. E. Western Blot Analysis After various treatments, the cells are washed twice with cold PBS, lysed in 100 pI of Laemmli buffer, scraped and heated for 5 min at 95 0 C. Total cell lysates are separated by SDS-PAGE and transferred to Immobilon-P membrane. The membrane is blocked for 30 minutes with PBS containing 0.1% Tween-20 and 3% skim milk and incubated for 1 hour at RT with a primary antibody diluted in blocking buffer. The membrane obtained is washed three times for 5 minutes with PBS containing 0.1% Tween-20 and incubated with peroxidase-conjugated secondary antibody for 1 hour at RT. After a washing step, the membrane is incubated for 1 minute with ECL reagent and exposed to film as required. For reprobing with another antibody, the membrane Is washed twice in PBS, stripped for 30 min at 55 0 C with stripping buffer (62.5 mM Tris-HOL, pH 6.8, 2% SDS, 100 mM 2 mercaptoethanol) and washed three times for 5 minutes with PBS at RT. The membrane is stored wet wrapped in SaranWrap at 4 0 C after each immunodetection. In vitro angiogenesis assay on Matrigel Morphogenesis of endothelial cells into capillary-like structures on Growth Factor Reduced Matrigel Matrix (BD Bioscience) is performed according to the manufacture procedure. Briefly, HUVEC are trypsinized, resuspended in serum-free M1 99 medium containing soybean trypsin inhibitor (1 mg/mI, Sigma). After centrifugation cells are resuspended in serum-free medium at a density 0.5 x 105 cells/ml, and cell suspension is seeded in 96-well cell culture plates (Costar, Coming Incorporated) precoated with 50 pl of Matrigel in the absence or presence of various stimuli: VEGF at 1.5 nM, S1P at 0.1-2 pM, S at 0.5-2 pM, Compound A at 0.5-2 pM, and Compound A-phosphate at 0.1-2 pM. Eight hours later, cells - 34 on Matrigel are fixed with 3 % formaldehyde In PBS and kept at 40C. Results are quantitated from images made with a Nikon Diaphot microscope equipped with a cooled CCD camera (Kappa GmbH, Gleichen, Germany) by direct counting of branching points on two microscopic fields from each well done in duplicates. F. Compound A-phosphate-induces morphogenesis of endothellal cells In in vitro tube formation assay on Matrigel and a possible involvment of Grmediated signaling pathway(s) The effect of Compound A and Compound A-phosphate on morphogenic differentiation of endothelial cells is determined using an in vitro angiogenesis assay on Matrigel. Endothelial cell morphogenesis Is a complex process that requires cell-extracellular matrix Interactions, followed by matrix remodelling, stimulated migration, cell-cell interactions, and perivascular proteolysis. As shown in Figure 1, Compound A-phosphate strongly may promote capillary like network formation in a bell-shape dose-dependent manner showing maxima! activity around 0.5 pM. The number of branching points per microscopic field, which reflects the induction potency of the stimulus, is comparable for Compound A-phosphate and S1 P, and may exceed significantly the VEGF-triggered effects. Compound A itself at 0.5 - 1 pM has a weak, in comparison to Compound A-phosphate, but consistent enhancing effect. Both Compound A-phosphate and Compound A at 0.5 - 1 pM does not attenuate VEGF-mediated remodelling but rather cooperates with polypeptide growth factor (see e.g. Figure 2). Furthermore, Compound A-phosphate- as well as S1P-stimulated tube formation is completely inhibited by pertussis toxin (PTX, 50 ng/ml), an inhibitor of heterotrimeric G proteins of rxv-type. This may be interpreted as a possible involvement of EDG-1 (S1 P 1 ) receptor-mediated signaling events in Compound A-phosphate-stimulated bioresponses (see e.g. Figure 3). S at 1 pM, which itself seems to be less potent than S1 P, attenuates the ability of both S1 P and Compound A-phosphate to induce capillary-like structures, without having an inhibitory effect on the VEGF-induced tube formation (see e.g. Figure 4). In this respect, S behaves differently from Compound A. The data indicate that the balance between S and S1 P seems to be critically important for endothelial cell activation/angiogenesis most likely via the EDG receptor family. Importantly, high concentrations of S and Compound A (2 - 5 pM) inhibited VEGF-triggered tube formation. That data suggest biphasic dose-dependent effects of Compound A and Compound

A

phosphate on angiogenesis in vitro. G. Activation of ERK1/2 MAP kinases by Compound A-phosphate - 35 Signal transduction via MAP kinases plays a key role in a variety of endothelial cell functions. Treatment of HUVEC with Compound A-phosphate at 0.5 pM may result in transient activation of ERK1/2 with a peak of phosphorylation/activation at 10 minutes and returning to baseline by 20 minutes (see e.g. Figure 5). No activation of p38 kinase and JNK1/2 by Compound A-phosphate Is detectable in HUVEC. Furthermore, Compound A-phosphate may trigger ERK1/2 activation In a dose-dependent manner, showing stronger activity at 2 pM. This is in contrast to the results from the tube formation assay, where Compound

A

phosphate at 2 pM may be less potent than at 0.5 pM. Neither Compound A nor S are able to induce MAP kinase activation in endothelial cells in a kinetics ranging from 5 minute to 60 minute treatment. To estimate the possible role of inflammatory/NF1cB-dependent program in Compound A-phosphate-stimulated bloresponses of endothelial cells, the membranes are reprobed with anti-IKB antibodies. IKB levels are not affected by Compound A-phosphate treatment. Moreover the treatment of endothellal cells with Compound A-phosphate may fail to induce E-Selectin expression as a NF-B-dependent secondary responsive gene. Thus, the data strongly indicate that Compound A-phosphate signaling does not involve NFCB activation - the main cascade In the acute inflammatory response in endothelial cells. H. Compound A and Compound A-phosphate do not induce tissue factor expression on endothelial cells An important characteristic feature of both classical inflammatory stimulus TNF-a and the main angiogenic growth factor VEGF on endothelial cells is their potency to upregulate tissue factor. Compound A, Compound A-phosphate, S or S1 P are tested whether they also do induce tissue factor on HUVEC. The data found demonstrate that none of these compounds alone or in combinations may elevate tissue factor activity (see e.g. Figure 6). Compound A and Compound A-phosphate may slightly enhance the VEGF- but not TNF-a induced tissue factor. The data obtained together Indicate that Compound A, Compound

A

phosphate, S and S1P mechanistically work distinctly to angiogenic VEGF and inflammatory TNF-ct. I. Binding affinity of S1P receptor agonists to individual human S1P receptors may be determined in following assays: Transient transfection of human S1 P receptors into HEK293 cells EDG receptors and Gi proteins are cloned, and equal amounts of 4 cDNAs for the EDG receptor, Gra, Grp and Gry are mixed and used to transfect monolayers of HEK293 cells using the calcium phosphate precipitate method (M. Wigler et al., Cell. 1977;11;223 and DS.

-36 Im et at., Mol. Pharmacol. 2000;57;753). Briefly, a DNA mixture containing 25 pg of DNA and 0.25 M GaCl is added to HEPES-buffered 2 mM Na 2 HPO4. Subconfluent monolayers of HEK293 cells are poisoned with 25 mM chloroquine, and the DNA precipitate Is then applied to the cells. After 4 h, the monolayers are washed with phosphate-buffered saline and reed media (90% 1:1 DulbeCCO's modified essential media (DMEM):F-1 2 + 10% f etal bovine serum). The cells are harvested 48-72 h after addition of the DNA by scraping in HME buffer (in mM: 20 HEPES, 5 MgCl2, 1 EDTA, pH 7.4) containing 10% sucrose on ice, and disrupted using a Dounce homogenizer. After centrifugation at 8OOxg, the supernatant is diluted with HME without sucrose and centrifuged at 100,oooxg for Ih. The resulting pellet is rehomogenized and centrifuged a second hour at 100,000xg. This crude membrane pellet Is suspended in HME with sucrose, aliquoted, and snap-frozen by immersion In liquid nitrogen. The membranes are stored at 700C. Protein concentration is determined spectroscopically by Bradford protein assay. GTP'S binding assay using Si P rece tor/HEK 293 mebra rations GTPyS binding experiments are performed as described by DS. Im et al., Mol. Pharmacol. 2000; 57:753. Ligand-mediated GTPyS binding to G-proteins is measured In GTP binding buffer (in mM: 50 HEPES, 100 NaCI, 10 MgC 2 , pH 7.5) using 25 pg of a membrane preparation from transiently transfected HEK293 cells. Ligand is added to membranes in the presence of 10 pM GDP and 0.1 nM [a 5 S]GTPtS (1200 Ci/mmol) and incubated at 300C for 30 min. Bound GTPyS is separated from unbound using the Brandel harvester (Gaithersburg, MD) and counted with a liquid scintillation counter. Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising" o will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps. The reference in this specification to any prior publication (or information derived from it), or to any matter which is known, is not, and should not be taken as an acknowledgment or admission or any form of suggestion that that prior publication (or information derived from it) or known matter forms part of the common general knowledge in the field of endeavour to which this specification relates.

Claims (18)

1. Use of a S1 P receptor agonist of formula 1 CH 2 0R 3 R 4 R 5 N CH

2 OR 2 I R1 5 wherein R 1 is a straight- or branched (C 12 - 22 )carbon chain - which may have in the chain a bond or a hetero atom selected from a double bond, a triple bond, 0, S, NR 6 , wherein R 6 is H, alkyl, aralkyl, acyl or alkoxycarbonyl, and carbonyl, and/or - which may have as a substituent alkoxy, alkenyloxy, alkynyloxy, aralkyloxy, 10 acyl, alkylamino, alkylthio, acylamino, alkoxycarbonyl, alkoxycarbonylamino, acyloxy, alkylcarbamoyl, nitro, halogen, amino, hydroxyimino, hydroxy or carboxy; or R 1 is - a phenylalkyl wherein alkyl is a straight- or branched (C 6 -2 0 )carbon chain; or 15 - a phenylalkyl wherein alkyl is a straight- or branched (C 1 . 30 )carbon chain wherein said phenylalkyl is substituted by - a straight- or branched (C 6 -2)carbon chain optionally substituted by halogen, - a straight- or branched (C 6 - 20 )alkoxy chain optionally substitued by halogen, 20 - a straight- or branched (C 6 - 20 )alkenyloxy, - phenylalkoxy, halophenylalkoxy, phenylalkoxyalkyl, phenoxyalkoxy or phenoxyalkyl, - cycloalkylalkyl substituted by C 6 -20alkyl, - heteroarylalkyl substituted by C 6 -20alkyl, 25 - heterocyclic C 6 -20alkyl or - heterocyclic alkyl substituted by C 2 - 20 alkyl, and wherein the alkyl moiety may have C.WRPorblDCCDARM37801592_1 DOC.2&D07/2011 - 38 - in the carbon chain, a bond or a heteroatom selected from a double bond, a triple bond, 0, S, sulfinyl, sulfonyl, or NR 6 , wherein R 6 is as defined above, and - as a substituent alkoxy, alkenyloxy, alkynyloxy, aralkyloxy, acyl, alkylamino, 5 alkylthio, acylamino, alkoxycarbonyl, alkoxycarbonylamino, acyloxy, alkylcarbamoyl, nitro, halogen, amino, hydroxy or carboxy, and each of R 2 , R 3 , R 4 , and R 5 , independently, is H, C 1 . 4 alkyl or acyl, or a pharmaceutically acceptable salt thereof, or a compound of formula IVa CH 2 R 3 a R 1 a I I_ (R 2 a) 2 N-C-CH 2 -Xa- P -o I I CH 2 Rib CH 2 I INa 10 (CH 2 ) 7 CH 3 wherein Xa is 0, S, NR 1 or a group -(CH2)na-, which group is unsubstituted or substituted by 1 to 4 halogen; na is 1 or 2, R 1 , is H or (C 1 .4)alkyl, which alkyl is unsubstituted or substituted by halogen; Ria is H, OH, (C 1 4)alkyl or O(C 1 .4)alkyl wherein alkyl is unsubstituted or substituted by 1 to 3 halogen; Rib is H, OH or 15 (C1.4)alkyl, wherein alkyl is unsubstituted or substituted by halogen; each R 2 a is independently selected from H or (C 1 4)alkyl, which alkyl is unsubstituted or substituted by halogen; R 3 a is H, OH, halogen or O(C 1 .4)alkyl wherein alkyl is unsubstituted or substituted by halogen, or a pharmaceutically acceptable salt or hydrate thereof; 20 for the preparation of a medicament for preventing or treating lymphoproliferative or myeloproliferative disorders, wherein the medicament is administered with a chemotherapeutic agent. C:\NRPonbnDCCDAR37K1"592-I DOC-21M7/2011 - 39 2. Use according to claim 1 wherein the lymphoproliferative disorder is tumors of blood or lymphatic system.

3. Use according to claim 1 wherein the myeloproliferative disorder is myeloid 5 cancer.

4. Use according to any one of the preceding claims wherein the medicament is administered intermittently. 10

5. Use according to any one of the preceding claims, wherein the SiP receptor agonist is 2-amino-2-[2-(4-octylphenyl)ethyl]propane-1,3-diol or a compound of formula IVa wherein R2a is H, R3a is OH, Xa is 0 and each of R1a and Rib is OH (called FTY720-phosphate), or a pharmaceutically acceptable salt thereof. 15

6. Use according to claim 5, wherein the S1 P receptor agonist is 2-amino-2-[2 (4-octylphenyl)ethyl]propane- 1, 3-diol, hydrochloride.

7. Use according to any one of the preceding claims, wherein the medicament 20 is administered concomitantly or in sequence with the chemotherapeutic agent.

8. Use according to any one of the preceding claims, wherein the chemotherapeutic agent is selected from i. an aromatase inhibitor, 25 ii. an antiestrogen, an anti-androgen or a gonadorelin agonist, iii. a topoisomerase I inhibitor or a topoisomerase 11 inhibitor, iv. a microtubule active agent, an alkylating agent, an antineoplastic antimetabolite or a platin compound, v. a compound targeting/decreasing a protein or lipid kinase activity or 30 a protein or lipid phosphatase activity, a further anti-angiogenic compound or a compound which induces cell differentiation C \NRPorbtDCC\DAR\37W592_ I DOC-2/07/201 1 - 40 processes, vi. a bradykinin 1 receptor or an angiotensin 11 antagonist, vii. a cyclooxygenase inhibitor, a bisphosphonate, a histone deacetylase inhibitor, a heparanase inhibitor, a biological response modifier, an 5 ubiquitination inhibitor, or an inhibitor which blocks anti-apoptotic pathways, viii. an inhibitor of Ras oncogenic isoforms, ix. a telomerase inhibitor, x. a protease inhibitor, a matrix metalloproteinase inhibitor, a 10 methionine aminopeptidase inhibitor, or a proteosome inhibitor, and/or xi. a mTOR inhibitor.

9. Use according to claim 8 wherein the chemotherapeutic agent is selected 15 from busulfan, cytarabine, 6-thioguanine, fludarabine, hydroxyurea, procarbazine, bleomycin, methotrexate, a topoisomerase 11 inhibitor, or a compound which targets, decreases or inhibits the activity of the platelet-derived growth factor receptors (PDGFR) or of c-Abl family member and their gene fusion products, and wherein the use is for treating or preventing tumors of blood or lymphatic system. 20

10. A method for preventing or treating lymphoproliferative or myeloproliferative disorders comprising administering a S1 P receptor agonist of formula I CH 2 0R 3 R 4 R 5 N CH 2 OR 2 R1 wherein R 1 is a straight- or branched (C 12 - 2 2 )carbon chain 25 - which may have in the chain a bond or a hetero atom selected from a double bond, a triple bond, 0, S, NR 6 , wherein R 6 is H, alkyl, aralkyl, acyl or alkoxycarbonyl, and carbonyl, and/or C\NRPonbl\DCC\DAR\3780592_ I DOC-2/7/20 l -41 - which may have as a substituent alkoxy, alkenyloxy, alkynyloxy, aralkyloxy, acyl, alkylamino, alkylthio, acylamino, alkoxycarbonyl, alkoxycarbonylamino, acyloxy, alkylcarbamoyl, nitro, halogen, amino, hydroxyimino, hydroxy or carboxy; or 5 R1 is - a phenylalkyl wherein alkyl is a straight- or branched (C 6 -2 0 )carbon chain; or - a phenylalkyl wherein alkyl is a straight- or branched (C 1 - 30 )carbon chain wherein said phenylalkyl is substituted by - a straight- or branched (C 6 - 2 o)carbon chain optionally substituted by 10 halogen, - a straight- or branched (C 6 -20)alkoxy chain optionally substitued by halogen, - a straight- or branched (C 6 - 20 )alkenyloxy, - phenylalkoxy, halophenylalkoxy, phenylalkoxyalkyl, phenoxyalkoxy or phenoxyalkyl, 15 - cycloalkylalkyl substituted by C 6 -20alkyl, - heteroarylalkyl substituted by C 6 -20alkyl, - heterocyclic C 6 -20alkyl or - heterocyclic alkyl substituted by C 2 -20alkyl, and wherein 20 the alkyl moiety may have - in the carbon chain, a bond or a heteroatom selected from a double bond, a triple bond, 0, S, sulfinyl, sulfonyl, or NR 6 , wherein R 6 is as defined above, and -- as a substituent alkoxy, alkenyloxy, alkynyloxy, aralkyloxy, acyl, alkylamino, 25 alkylthio, acylamino, alkoxycarbonyl, alkoxycarbonylamino, acyloxy, alkylcarbamoyl, nitro, halogen, amino, hydroxy or carboxy, and each of R 2 , R 3 , R 4 , and R 5 , independently, is H, C1.4alkyl or acyl, or a pharmaceutically acceptable salt thereof, or a compound of formula IVa C:\NRPorb\DCC\DAR\37H0592_ I DOC-2MA712011 -42 CH 2 R 3 a R 1 a I I (R 2 a) 2 N-C-CH 2 -Xa-P o I I CH 2 Rib CH 2 INa (CH 2 ) 7 CH 3 wherein Xa is 0, S, NR 1 s or a group -(CH2)na-, which group is unsubstituted or substituted by 1 to 4 halogen; na is 1 or 2, R 1 , is H or (C 1 .4)alkyl, which alkyl is unsubstituted or substituted by halogen; Ria is H, OH, (C1.4)alkyl or O(C 1 .4)alkyl 5 wherein alkyl is unsubstituted or substituted by 1 to 3 halogen; Rib is H, OH or (C 1 .4)alkyl, wherein alkyl is unsubstituted or substituted by halogen; each R 2 a is independently selected from H or (C 1 .4)alkyl, which alkyl is unsubstituted or substituted by halogen; R 3 a is H, OH, halogen or O(C 1 4)alkyl wherein alkyl is unsubstituted or substituted by halogen, 10 or a pharmaceutically acceptable salt or hydrate thereof; and a chemotherapeutic agent to a subject in need thereof.

11. The method according to claim 10 wherein the lymphoproliferative disorder is tumors of blood or lymphatic system. 15

12. The method according to claim 10 wherein the myeloproliferative disorder is myeloid cancer.

13. The method of any one of claims 10 to 12 wherein the S1P receptor agonist 20 is administered intermittently.

14. The method of any one of claims 10 to 13 wherein the S1P receptor agonist is 2-amino-2-[2-(4-octylphenyl)ethyl]propane-1,3-diol or a compound of formula C:WNRPonblDCCDAR378)592_I.DOC.28107/201 1 -43 IVa wherein R 2 a is H, R 3 a is OH, Xa is 0 and each of RI, and Rib is OH (called FTY720-phosphate), or a pharmaceutically acceptable salt thereof.

15. The method of claim 14 wherein the S1 P receptor agonist is 2-amino-2-[2 5 (4-octylphenyl)ethyl]propane-1,3-diol, hydrochloride.

16. The method of any one of claims 10 to 15 wherein the medicament is administered concomitantly or in sequence with the chemotherapeutic agent. 10

17. The method of any one of claims 10 to 16 wherein the chemotherapeutic agent is selected from i. an aromatase inhibitor, ii. an antiestrogen, an anti-androgen or a gonadorelin agonist, iii. a topoisomerase I inhibitor or a topoisomerase 11 inhibitor, 15 xi. a microtubule active agent, an alkylating agent, an antineoplastic antimetabolite or a platin compound, xii. a compound targeting/decreasing a protein or lipid kinase activity or a protein or lipid phosphatase activity, a further anti-angiogenic compound or a compound which induces cell differentiation 20 processes, xiii. a bradykinin 1 receptor or an angiotensin 11 antagonist, xiv. a cyclooxygenase inhibitor, a bisphosphonate, a histone deacetylase inhibitor, a heparanase inhibitor, a biological response modifier, an ubiquitination inhibitor, or an inhibitor which blocks anti-apoptotic 25 pathways, xv. an inhibitor of Ras oncogenic isoforms, xvi. a telomerase inhibitor, xvii. a protease inhibitor, a matrix metalloproteinase inhibitor, a methionine aminopeptidase inhibitor, or a proteosome inhibitor, 30 and/or xi. a mTOR inhibitor. C \NRPornbl\DCC\DAR\378)592_1 DOC-28I7/201 I - 44

18. The method of claim 17 wherein the chemotherapeutic agent is selected from busulfan, cytarabine, 6-thioguanine, fludarabine, hydroxyurea, procarbazine, bleomycin, methotrexate, a topoisomerase 11 inhibitor, or a compound which 5 targets, decreases or inhibits the activity of the platelet-derived growth factor receptors (PDGFR) or of c-Abl family member and their gene fusion products, and wherein the use is for treating or preventing tumors of blood or lymphatic system.