AU2010276043A1

AU2010276043A1 - Methods of making Efavirenz and intermediates thereof

Info

Publication number: AU2010276043A1
Application number: AU2010276043A
Authority: AU
Inventors: Bo Chen; Hao Chen; Lihong Liu; Zhi-Xian Wang; Yutao Xue
Original assignee: Apotex Pharmachem Inc
Current assignee: Apotex Pharmachem Inc
Priority date: 2009-07-20
Filing date: 2010-07-20
Publication date: 2012-02-23
Also published as: EP2456773A1; US20110015189A1; WO2011009203A1; US8378143B2; EP2456773A4; US20120095249A1; CA2768712A1; US8080655B2

Abstract

The present invention is directed to methods of preparation of Efavirenz ( 1 ),various intermediates useful in the preparation of Efavirenz and methods of preparation of such intermediates hi some embodiments, a stereoselective cyclopropylacetylide addition reaction may be controlled by introduction of an appropriate chiral carbonyl auxiliary group on the aniline nitrogen The product of such an aymmetric addition (5) may then undergo a cyclization reaction with concomitant removal of the chiral auxiliary group, without the need for a discrete deprotection step. Formulae (I), (II).

Description

WO 2011/009203 PCT/CA2010/001124 1 METHODS OF MAKING EFAVIRENZ AND INTERMEDIATES THEREOF TECHNICAL FIELD This invention relates to the field of chemical synthesis of organic 5 compounds and in particular to a synthesis of Efavirenz and intermediates thereof. BACKGROUND A common feature of retrovirus replication is reverse transcription of 10 the RNA genome by a virally encoded reverse transcriptase to generate DNA copies of human immunodeficiency virus (HIV) sequences. Therefore, reverse transcriptase is a clinically relevant target for the chemotherapy of retroviral infections. It is known that a number of benzoxazinone compounds are effective in 15 the treatment of HIV which is the retrovirus that causes progressive destruction of the human immune system with the resultant onset of AIDS. Among them, the (S)-6-chloro-4-cyclopropylethynyl-4-trifluoromethyl-1,4-dihydro-2H-3,1 -benzo xazin-2-one of Formula 1 (Efavirenz) is not only a highly potent reverse 20 transcriptase inhibitor, but also efficacious against HIV reverse transcriptase resistance. C1

F

3 CIo H Efavirenz (1) US 5,519,021 discloses certain benzoxazinones that are useful in the 25 inhibition of HIV reverse transciptase (including its resistant varieties), the prevention or treatment of infection by HIV and the treatment of AIDS, either as compounds, pharmaceutically acceptable salts, pharmaceutical composition ingredients, whether or not in combination with other antivirals, immunomodulators, antibiotics or vaccines. Methods of treating AIDS and 30 methods of preventing or treating infection by HIV are also described.

WO 2011/009203 PCT/CA2010/001124 2 In US 5,633,405 an improved synthesis of a highly potent HIV reverse transcription inhibitor is disclosed, involving an acetylide and a trifluoromethyl ketone which produces a chiral product in the presence of a chiral amino alcohol. See also Tetrahedron Lett. 1995, 36, 8937 and J.Org. Chem. 1998, 5 63, 8536. US 5,922,864 discloses an efficient method for the preparation of a compound of (-)-6-chloro-4-cyclopropylethynyl-4-trifluoromethyl-1,4-dihydro-2H-3, 1-benzoxazin-2-one, also known as DMP-266, a reverse transcriptase 10 inhibitor using a cyclization reaction of the amino alcohol intermediate with an alkyl or aryl chloroformate and a base. US 5,925,789 provides novel methods for the asymmetric synthesis of (S)-6-chloro-4-cyclopropylethynyl-4-trifluoromethyl-1,4-dihydro-2H-3, 1 -benzox azin-2-one of Formula 1 which is useful as a human immunodeficiency virus 15 (HIV) reverse transcriptase inhibitor. US 5,952,528 discloses a process for enhancing the purity of 2R-[1 -hydroxy-1 -trifluoromethyl-3-cyclopropylpropyn-2-yl]-4-chloroaniline comprising the formation of an acid addition salt which is capable of rejecting the racemate in the selected organic solvent. 20 US 6,015,926 discloses an efficient method for the preparation of key intermediate, in the synthesis of (-)-6-chloro-4-cyclopropylethynyl-4-trifluoromethyl-1,4-dihydro-2H-3,1 -benzoxa zin-2-one, a reverse transcriptase inhibitor is achieved using a chiral addition reaction to the 4-chloro-2-trifluoromethylketoaniline with an organozinc 25 complex to give the desired alcohol. This instant method has broad applicability in the chiral addition to any prochiral ketone. US 7,205,402 provides novel methods for the synthesis of (S)-6-chloro-4-cyclopropyethynyl-4-trifluoromethyl-1,4-dihydro-2H-3,1 -benzox azin-2-one of Formula I which is useful as a human immunodeficiency virus 30 (HIV) reverse transcriptase inhibitor. US 7,439,400 disclosed a new process of asymmetric alkynylation of ketone or ketimine, involving the chiral ligand-mediated asymmetric addition of zinc or copper acetylide to a trifluoromethyl ketone or ketimine intermediate to give a chiral tertiary propargylic alcohols or amines. The adduct WO 2011/009203 PCT/CA2010/001124 3 compounds include the key precursors to the potent HIV reverse transcriptase inhibitor Efavirenz (DMP 266), DPC 961 and DPC 083. The invention also disclosed a novel chiral amino ligand. 5 SUMMARY The present invention is directed to methods of preparation of Efavirenz, various intermediates useful in the preparation of Efavirenz and methods of preparation of such intermediates. In some embodiments, the present invention allows for removal of the 10 aniline auxiliary group of a compound of Formula 5 during the cyclization step without additional chemical reagent or treatment. In some embodiments, the stereoselectivity of the cyclopropylacetylide reaction may be controlled by introduction of an appropriate chiral carbonyl auxiliary group on the aniline nitrogen. The product of such an asymmetric 15 addition may then undergo a cyclization reaction with concomitant removal of the chiral auxiliary group, without the need for a discrete deprotection step. In illustrative embodiments of the present invention there is provided a C1F 3 C K ~N(S)O C NlO process for the preparation of a compound of Formula 1: H the process comprising cyclizing, in the presence of a first base, a compound F3C C --OH 0 N R 1 H 20 of Formula 5: 5, with a haloformate of Formula 6: 0 R3 isatX 6 ,wherein R' is alkyl, substituted alkyl, aryl, substituted aryl, WO 2011/009203 PCT/CA2010/001124 4 arylalkyl, substituted arylalkyl or a chiral auxiliary group; R 3 is alkyl, substituted alkyl, aryl, substituted aryl, arylalkyl, or substituted arylalkyl; and X is halogen. In illustrative embodiments of the present invention there is provided a process described herein further comprising treatment with a second base. 5 In illustrative embodiments of the present invention there is provided a process described herein wherein an intermediate of Formula 7:

F

3 C O ClO OR 3 0 N'k R H 7 is isolated before treatment with the second base, wherein R 1 is alkyl, substituted alkyl, aryl, substituted aryl, arylalkyl, substituted arylalkyl or a chiral auxiliary group; and R 3 is alkyl, substituted alkyl, aryl, 10 substituted aryl, arylalkyl, or substituted arylalkyl. In illustrative embodiments of the present invention there is provided a process described herein wherein the second base is selected from the group consisting of sodium hydroxide, potassium hydroxide, lithium hydroxide, sodium carbonate, potassium carbonate, lithium carbonate, triethylamine 15 diisopropylethylamine, N,N-dimethylaniline, and N,N-diethylaniline, pyridine and mixtures thereof. In illustrative embodiments of the present invention there is provided a process described herein wherein R' is selected from the group consisting of 20 methyl, ethyl, isobutyl, tert-butyl, and benzyl. In illustrative embodiments of the present invention there is provided a process described herein wherein R' is a chiral auxiliary group. In illustrative embodiments of the present invention there is provided a process described herein wherein the compound of Formula 5 is 25 (1 S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1 -trifluoro-2-hydroxybut-3-yn-2-yl )phenyl)-4,7,7-trimethyl-3-oxo-2-oxAbicyclo{2.2.1 ]heptane-1 -carboxamide.

WO 2011/009203 PCT/CA2010/001124 5 In illustrative embodiments of the present invention there is provided a process described herein wherein the compound of Formula 5 is (R)-2-(4-chloro-2-((S)-4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut-3-yn-2-yl)phe nylamino)-2-oxo-1-phenylethyl pivalate. 5 In illustrative embodiments of the present invention there is provided a process described herein wherein the haloformate of Formula 6 is selected from the group consisting of 4-nitrophenyl haloformate, 4-chlorophenyl haloformate, phenyl haloformate and 1-chloroethyl haloformate. In illustrative embodiments of the present invention there is provided a 10 process described herein wherein the first base is selected from the group consisting of sodium hydroxide, potassium hydroxide, lithium hydroxide, sodium carbonate, potassium carbonate, lithium carbonate, triethylamine diisopropylethylamine, N,N-dimethylaniline, and N,N-diethylaniline, pyridine and mixtures thereof. 15 In illustrative embodiments of the present invention there is provided a

F

3 C OH O N R H process for making a compound of Formula 5: 5 ,the process comprising: i) reacting a compound of Formula 2: 0

CF

3

NH

2 2 or an acid addition salt thereof, with a chiral acylating agent of Formula R 2 COG to form a compound of Formula 3, Formula 3a or a WO 2011/009203 PCT/CA2010/001124 6 0 0 CI CF 3 CI / CF 3 o 0 VN k-R2 N- 'R2 mixture thereof: 3a ii) reacting the compound of Formula 3, Formula 3a or the mixture thereof with a ~- M compound of Formula 4: 4 to form the compound of Formula 5, wherein R 2 is a chiral auxiliary group; G is a hydroxyl group, or a leaving 5 group; and M is a metal. In illustrative embodiments of the present invention there is provided a process described herein wherein G is chloro or the hydroxyl group and R 2 is: 0 X0 In illustrative embodiments of the present invention there is provided a Ph 0 O 10 process described herein wherein G is chloro and R 2 is: In illustrative embodiments of the present invention there is provided a process described herein wherein R 2 is: OR 4 , R 4 is a hydroxyl protecting group; and the carbon center designated "*" is enantiomerically enriched in a (R)-or (S)-configuration. 15 In illustrative embodiments of the present invention there is provided a process described herein wherein M is lithium, sodium, potassium, magnesium halide or mixtures thereof.

WO 2011/009203 PCT/CA2010/001124 7 In illustrative embodiments of the present invention there is provided a process described herein wherein M is lithium. In illustrative embodiments of the present invention there is provided a process for the preparation of a compound of Formula 1: C1 0, N(S)O 5 H ,the process comprising: i) reacting a compound of 0 C 1 C F 3

NH

2 Formula 2: 2 or an acid addition salt thereof, with a chiral acylating agent of Formula R 2 COG to form a compound of Formula 3, Formula 3a or a mixture thereof: 0 0 C1 CF 3 C1 CF 3 o O N LR2 NJL R2 H 3

R

2 O 3a ; ii) reacting the compound 10 of Formula 3, Formula 3a or the mixture thereof with a compound of Formula M 4: 4 , to form a compound of Formula 5:

F

3 C C1 OH H 5 ;iii) hydrolysing the compound of Formula 5 to form WO 2011/009203 PCT/CA2010/001124 8

F

3 C OH

NH

2 a compound of Formula 8: 8 ; and iv) cyclizing the compound of Formula 8 to give the compound of Formula 1, wherein R' and R 2 are a chiral auxiliary group; G is a hydroxyl group, or a leaving group; and M is a metal. 5 In illustrative embodiments of the present invention there is provided a process described herein wherein G is chloro or the hydroxyl group and R 1 and R2 are: 0. In illustrative embodiments of the present invention there is provided a Ph , 0 0 process described herein wherein G is chloro and R and R are: 10 In illustrative embodiments of the present invention there is provided a process described herein wherein RI and R 2 are: OR 4 , R 4 is a hydroxyl protecting group; and the carbon centre designated "*" is enantiomerically enriched in a (R)- or (S)-configuration. In illustrative embodiments of the present invention there is provided a 15 process described herein wherein M is lithium, sodium, potassium, magnesium halide or a mixture thereof. In illustrative embodiments of the present invention there is provided a process described herein wherein M is lithium.

WO 2011/009203 PCT/CA2010/001124 9 In illustrative embodiments of the present invention there is provided a process described herein wherein the cyclization of a compound of Formula 8 to give the compound of Formula 1 is performed with a cyclization reagent selected from the group consisting of C1C10 alkyl haloformates, C6-C12 aryl 5 haloformates, phosgene, triphosgene and 1,1'-carbonyldiimidazole. In illustrative embodiments of the present invention there is provided a 0 CI /CF 3 N 0 H compound of Formula 3: 3 , wherein R 2 is a chiral auxiliary group. In illustrative embodiments of the present invention there is provided a 0 C1

CF

3 | 0 N)<R2 10 compound of Formula 3a: 3a , wherein R 2 is a chiral auxiliary group. In illustrative embodiments of the present invention there is provided a

F

3 C / OH H compound of Formula 5: 5, wherein R' is a chiral auxiliary group. 15 In illustrative embodiments of the present invention there is provided a process described herein wherein the chiral auxilliary group is Q of a chiral acid QCOOH, wherein QCOOH is selected from the group consisting of: WO 2011/009203 PCT/CA2010/001124 10 natural chiral organic acids, natural chiral organic acid derivatives, unnatural chiral organic acids, unnatural chiral organic acid derivatives, natural amino acid derivatives, and unnatural amino acid derivatives, In illustrative embodiments of the present invention there is provided a 5 process described herein wherein the chiral auxiliary group is a chiral C 1 -C1O alkoxyl or a chiral C6-C12 aralkoxyl group. In illustrative embodiments of the present invention there is provided a 0 CI /CF 3 NH 0 0 compound of Formula 9: 9 In illustrative embodiments of the present invention there is provided a

F-

3 C Cl o C1 OH NH 0 0 0 10 compound of Formula 10: 10 WO 2011/009203 PCT/CA2010/001124 11 In illustrative embodiments of the present invention there is provided a 0 CI C1

HCF

3 O o 00 compound of Formula 11: 11 In illustrative embodiments of the present invention there is provided a 0 3

CF

3 NH Ph O 0 0 compound of Formula 12: 12 5 In illustrative embodiments of the present invention there is provided a CI3 C1 OH NH Ph 0 0 compound of Formula 13: 13 WO 2011/009203 PCT/CA2010/001124 12 In illustrative embodiments of the present invention there is provided a 0 Cl HeCF 3 NH Ph O

OR

4 compound of Formula 14: 14 wherein R 4 is a hydroxyl protecting group; and the carbon centre designated "*" is enantiomerically enriched in a (R)- or (S)-configuration. 5 In illustrative embodiments of the present invention there is provided a F3C CI OH NH Ph O

OR

4 compound of Formula 15: 15 ,wherein R 4 is a hydroxyl protecting group; and the carbon centre designated "*" is enantiomerically enriched in a (R)- or (S)-configuration. In illustrative embodiments of the present invention there is provided a

F

3 C / O 0C1 OR 3 N' R 10 compound of Formula 7: H ,wherein R' is alkyl, substituted alkyl, aryl, substituted aryl, arylalkyl, substituted arylalkyl or a chiral auxiliary group; and R 3 is alkyl, substituted alkyl, aryl, substituted aryl, arylalkyl, or substituted arylalkyl.

WO 2011/009203 PCT/CA2010/001124 13 In illustrative embodiments of the present invention there is provided a C1F 3 C7/ (S)O c NiO composition comprising a compound of Formula 1: H and a

F

3 C Y/ c1 0 OR 3 0 ' NNk R H compound of Formula 7: 7 In illustrative embodiments of the present invention there is provided a

F

3 C 7' C1 (S)Q 5 composition comprising a compound of Formula 1: H and a compound of Formula 5: C1 OH 10 H 5 Other aspects and features of the present invention will become apparent to those ordinarily skilled in the art upon review of the following description of 10 specific embodiments of the invention in conjunction with the accompanying figures.

WO 2011/009203 PCT/CA2010/001124 14 DETAILED DESCRIPTION As used herein, the term "substituted" refers to the replacement of a hydrogen atom on a compound with a substituent group. A substituent may be a non-hydrogen atom or multiple atoms of which at least one is a 5 non-hydrogen atom and one or more may or may not be hydrogen atoms. For example, without limitation, substituted compounds may comprise one or more substituents selected from the group consisting of: R", OR", NR"R"', SR", halogen, SiR"R"'R"", OC(O)R", C(O)R", CO 2 R", CONR"R',

NR"'C(O)

2 R", S(O)R", S(O) 2 R", CN and NO 2 , 10 As used herein, each R", R"', and R"" may be selected, independently, from the group consisting of: hydrogen, halogen, oxygen, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted alkyl, alkoxy or thioalkoxy groups, and arylalkyl groups. As used herein, the term "alkyl" by itself or as part of another 15 substituent, means, unless otherwise stated, a straight or branched chain, or cyclic hydrocarbon radical, or combination thereof, which may be fully saturated, mono- or polyunsaturated and can include di- and multivalent radicals, having the number of carbon atoms designated (e.g. CI-C1o or 1- to 10-membered means one to ten carbons). Examples of saturated 20 hydrocarbon radicals include, but are not limited to, groups such as methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, isobutyl, sec-butyl, cyclohexyl, (cyclohexyl)methyl, cyclopropylmethyl, homologs and isomers of, for example, n-pentyl, n-hexyl, n-heptyl, n-octyl, and the like. An unsaturated alkyl group is one having one or more double bonds or triple bonds. Examples of 25 unsaturated alkyl groups include, but are not limited to, vinyl, 2-propenyl, crotyl, 2-isopentenyl, 2-(butadienyl), 2,4-pentadienyl, 3-(1,4-pentadienyl), ethynyl, 1- and 3-propynyl, 3-butynyl, and the higher homologs and isomers. As used herein, the term "aryl" by itself or as part of another substituent, means, unless otherwise stated, a polyunsaturated, aromatic, 30 hydrocarbon substituent which can be a single ring or multiple rings (often from 1 to 3 rings) which are fused together or linked covalently. "Aryl" includes, but is not limited to, "heteroaryl" groups. "Heteroaryl" refers to an aryl group that contain from one to four heteroatoms selected from N, 0, and WO 2011/009203 PCT/CA2010/001124 15 S, wherein the nitrogen and sulfur atoms are optionally oxidized, and the nitrogen atom(s) are optionally quaternized. A heteroaryl group can be attached to the remainder of the molecule through a heteroatom. Non-limiting examples of aryl and heteroaryl groups include: phenyl, 1-naphthyl, 5 2-naphthyl, 4-biphenyl, 1-pyrrolyl, 2-pyrrolyl, 3-pyrrolyl, 3-pyrazolyl, 2-imidazolyl, 4-imidazolyl, pyrazinyl, 2-oxazolyl, 4-oxazolyl, 2-phenyl-4-oxazolyl, 5-oxazolyl, 3-isoxazolyl, 4-isoxazolyl, 5-isoxazolyl, 2-thiazolyl, 4-thiazolyl, 5-thiazolyl, 2-furyl, 3-furyl, 2-thienyl, 3-thienyl, 2-pyridinyl, 3-pyridinyl, 4-pyridinyl, 2-pyrimidyl, 4-pyrimidyl, 5-benzothiazolyl, 10 purinyl, 2-benzimidazolyl, 5-indolyl, 1-isoquinolyl, 5-isoquinolyl, 2-quinoxalinyl, 5-quinoxalinyl, 3-quinolyl, and 6-quinolyl. The term "aryl" when used in combination with other terms (e.g., aryloxy, arylthioxy, arylalkyl) includes both aryl and heteroaryl rings as defined above. Thus, the term "arylalkyl" is meant to include those radicals in which an aryl group is attached to an alkyl group 15 (e.g., benzyl, phenethyl, pyridylmethyl, etc.) including those alkyl groups in which a carbon atom containing group (e.g., a methylene group) has been replaced by, for example, an oxygen atom (e.g., phenoxymethyl, 2-pyridyloxymethyl, 3-(1-naphthyloxy)propyl, etc). As used herein the term "leaving group" refers to a halogen atom (e.g. 20 chlorine, bromine and iodine) and/or sulfonyloxy groups (e.g. methanesufonyloxy, trifluoromethanesulfonyloxy, p-toluenesulfonyloxy). As used herein, the term "chiral auxiliary group" refers to any chiral chemical compound or unit that is temporarily incorporated into an organic synthesis for the purpose of altering the stereochemical outcome of a 25 subsequent reaction. Chiral auxiliaries are optically active compounds and introduce chirality in otherwise racemic compounds. After it has served its purpose the chiral auxiliary can be removed in a later step and recycled. As used herein, "hydroxyl protecting group" refers to a protecting group that is introduced into a molecule by chemical modification of a hydroxy group 30 in order to obtain chemoselectivity in a subsequent chemical reaction. Such hydroxyl protecting groups include those listed in Greene, T. W. and Wuts, P. G. M., "Chapter 2, Protection for the hydroxyl group, including 1,2- and 1,3-diols", in "Protective Groups in Organic Synthesis", Fourth Edition, John Wiley & Sons, Inc., 2007, pp. 16-366. Hydroxyl protecting group includes, but WO 2011/009203 PCT/CA2010/001124 16 is not limited to 1) ethers, including silyl ethers such as trimethylsilyl (TMS) ether, tert-butyldimethylsilyl (TBDMS) ether, and triisopropylsilyl (TIPS) ether, tetrahydropyran (THP), P-methoxyethoxymethyl ether (MEM), p-methoxybenzyl ether (PMB), methoxymethyl ether (MOM), ethoxyethyl 5 ethers (EE), methyl ether, benzyl ether, methylthiomethyl ether; and 2) esters such as pivaloyl (Piv) esters. According to illustrative embodiments of the present invention, there is provided a process for the preparation of a compound of Formula 1:

F

3 C C1 (S)Q H 10 1 comprising cyclization, in the presence of a first base, of a compound of Formula 5:

F

3 C 7 C1 OH 0 NC R 1 H 5 with a haloformate of Formula 6: 0 15 6 wherein

R

1 is CI-C1o alkyl, substituted C1-C10 alkyl, C 6

-C

12 aryl, substituted

C

6 -C1 2 aryl, C6-C12 arylalkyl, substituted C6-C12 arylalkyl or a chiral auxiliary group; 20 R 3 is CI-C1o alkyl, substituted C1-C10 alkyl, C6-C12 aryl, substituted C6-C12 aryl, C6-C12 arylalkyl, or substituted C6-C12 arylalkyl; and WO 2011/009203 PCT/CA2010/001124 17 X is halogen; optionally followed by treatment with a second base. In some embodiments of Formula 5, R 1 is selected from the group consisting of C-C1O alkyl, substituted C1-C0 alkyl, C6-C12 aryl, substituted 5 C6-C12 aryl, C6-C12 arylalkyl and substituted C6-C12 arylalkyl. In some embodiments of Formula 5, R 1 is selected from the group consisting of methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, cyclopentyl, cyclohexyl, hexyl, phenyl, and benzyl. In some embodiments of Formula 5, R, is selected from the group consisting of methyl, ethyl, isobutyl, tert-butyl, and 10 benzyl. In some embodiments of Formula 5, R 1 is a chiral auxiliary group. In some embodiments of Formula 5 the chiral auxiliary group is Q of a chiral acid QCOOH, wherein QCOOH is selected from natural chiral organic acids, natural chiral organic acid derivatives, unnatural chiral organic acids, unnatural chiral organic acid derivatives, natural amino acid derivatives, and 15 unnatural amino acid derivatives. In some embodiments of Formula 5, R 1 is Q of a chiral acid QCOOH, wherein QCOOH is a chiral acid selected from the group consisting of camphanic acid, 2-pyrrolidone-5-carboxylic acid, naproxen, ibuprofen; tartaric acid, malic acid, lactic acid, 3-hydroxybutyric acid, mandelic acid or derivatives thereof. In other embodiments of Formula 20 5, the chiral auxiliary group is a chiral alkoxyl or chiral aralkoxyl group such as the menthoxy or camphanoxy groups: or camphanoxy menthoxy In some embodiments of Formula 5 the chiral auxiliary group is one of the following two groups: 25 WO 2011/009203 PCT/CA2010/001124 18

H

3 C CH 3 R4

H

3 C '6 wherein R 4 is a hydroxyl protecting group, and the carbon centre designated is enantiomerically enriched in a (R)- or (S)-configuration. In some embodiments, the haloformate of Formula 6 is selected from 5 the group consisting of C1-CO alkyl haloformate, substituted C1-C10 alkyl haloformate, C6-C12 aryl haloformate, and substituted C6-C12 aryl haloformate. In some embodiments, the haloformate of Formula 6 is selected from the group consisting of methyl haloformate, ethyl haloformate, hydroxymethyl haloformate, hydroxyethyl haloformate, 1-chloroethyl haloformate, 2 10 chloroethyl haloformate, phenyl haloformate, hydroxyphenyl haloformate, dihydroxyphenyl haloformate, trihydroxyphenyl haloformate, methyoxyphenyl haloformate, chlorophenyl haloformate, dichlorophenyl haloformate, trichlorophenyl haloformate, pentachlorophenyl haloformate and 4-nitrophenyl haloformate. In some embodiments, the haloformate of Formula 6 is selected 15 from the group consisting of 4-nitrophenyl haloformate, phenyl haloformate and 1-chloroethyl haloformate. The first and second base may be the same or different. The first and/or second base may be any of a variety of bases which facilitates the desired reaction. The first and/or second base may be an organic or 20 inorganic base. The first and/or second base may be selected from the group consisting of sodium hydroxide, potassium hydroxide, lithium hydroxide, sodium carbonate, potassium carbonate, lithium carbonate, triethylamine, diisopropylethylamine, N,N-dimethylaniline, N,N-diethylaniline, pyridine and mixtures thereof. The first and/or second base may be triethylamine, 25 diisopropylamine, sodium hydroxide or potassium carbonate. In some embodiments, the cyclization is a step-wise process, whereby the compound of Formula 5 is treated with the haloformate of Formula 6 in the presence of the first base to form an intermediate carbonate of Formula 7: WO 2011/009203 PCT/CA2010/001124 19

F

3 C 0 OR 3 0 N

R

1 H 7 wherein R 1 may be as defined above for Formula 5 and R 3 may be as defined above for Formula 6. The intermediate carbonate of Formula 7 is subsequently treated with the second base to induce cyclization. The 5 intermediate carbonate of Formula 7 may or may not be isolated before treatment with the second base. The isolation of the carbonate of Formula 7 also provides for an additional opportunity to purify and in some cases may provide for a purer product. In a step-wise process, the same solvent may be used throughout or a different solvent may be used for each step. 10 Since the same solvent may be used throughout, in some embodiments, the cyclization may also be carried out in a one-step and/or a one-pot process, whereby cyclization of the compound of Formula 5 occurs upon treatment with the haloformate in the presence of the first base. In some embodiments, the first base may be added in a portion wise process. 15 The cyclization may occur in a first solvent. The first solvent may be selected from group consisting of C1-C10 alkyl ethers (e.g. diethyl ether, methyl t-butyl ether, diisopropyl ether, butyl ether), C1-C10 alkyl esters (e.g. ethyl acetate), C1-C10 ketones (e.g. acetone, methyl ethyl ketone, methyl isobutyl ketone), C6-C12 aromatic hydrocarbons and C1-C1O aliphatic 20 hydrocarbons (e.g. toluene, xylenes, hexanes and heptanes), C1-C12 nitriles, (e.g. acetonitile, propionitrile, butyronitrile, and benzonitrile) Ci-C12 N,N-dialkylamides (e.g. N,N-dimethylformamide, N,N-dimethylacetamide and N-methyl-2-pyrrolidinone), C1-C10 sulfoxides and C1-C10 sulfones (e.g. dimethyl sulfoxide and sulfolane), halogenated C1-CO hydrocarbons 25 (e.g.dichloromethane and dichloroethane), water and mixtures thereof. The WO 2011/009203 PCT/CA2010/001124 20 first solvent may be toluene, dichloromethane, methyl t-butyl ether or N,N-dimethylformamide. The compound of Formula 5, may be prepared by acylation of a compound of Formula 8:

F

3 C 7 CIOH

NH

2 5 8 according to known methods in the art, for example, see Francis A. Carey and Richard J. Sundberg, "Advanced Organic Chemistry, Part B: Reactions and Synthesis", Fifth Edition, Springer, 2007, pages 252-258. The compound of Formula 8 may be prepared according to the method described in this 10 invention or other methods known in the art. According to illustrative embodiments of the present invention there is provided a process for the preparation of a compound of Formula 5 wherein

R

1 is a chiral auxiliary group, the process comprising: i) reacting a compound of Formula 2: 0

CF

3

NH

2 15 2 or an acid addition salt thereof, with a chiral acylating agent of Formula

R

2 COG to form a compound of Formula 3, 3a or mixtures thereof: 0 0 CI CF 3 a CF 3 o 0 N'k R2 N R2 3 R2>O 3a ;and WO 2011/009203 PCT/CA2010/001124 21 ii) reacting the compound of Formula 3, 3a or mixtures thereof with a compound of Formula 4: ~- M 4 to give a compound of Formula 5, 5 wherein

R

2 is a chiral auxiliary group; G is a hydroxyl group, or a leaving group; and M is a metal. In some embodiments of Formula 3 and/or 3a, R 2 is Q of a chiral acid 10 QCOOH, wherein QCOOH is selected from natural chiral organic acids, natural chiral organic acid derivatives, unnatural chiral organic acids, unnatural chiral organic acid derivatives, natural amino acid derivatives, and unnatural amino acid derivatives. In other embodiments of Formula 3 and/or 3a, the chiral auxiliary group is a chiral alkoxyl or chiral aralkoxyl group such 15 as the menthoxy or camphanoxy group. In some embodiments of Formula 3 and/or 3a, R 2 is Q of a chiral acid QCOOH, wherein QCOOH is a chiral acid selected from the group consisting of camphanic acid, 2-pyrrolidone-5-carboxylic acid, naproxen, ibuprofen; tartaric acid, malic acid, lactic acid, 3-hydroxybutyric acid, mandelic acid and derivatives thereof. In 20 some embodiments of Formula 3 and/or 3a, R 2 is one of the following two groups:

H

3 C CH 3 'R4

H

3 Co wherein R 4 is a hydroxyl protecting group, and the carbon centre designated "*" is enantiomerically enriched in a (R)- or (S)-configuration. 25 The leaving group G may be independently selected from the group consisting of: halogen atoms (e.g. chlorine, bromine and iodine) and sulfonyioxy groups (e.g. methanesulfonyloxy, trifluoromethanesulfonyloxy, p-toluenesulfonyloxy). In some embodiments, G is chlorine.

WO 2011/009203 PCT/CA2010/001124 22 The chiral acylating agent of Formula R 2 COG may be (-)-camphanic acid, (-)-camphanoyl chloride, or (R)-2-chloro-2-oxo-1-phenethyl pivalate. The reaction of the compound of Formula 2 and the chiral acylating agent may occur in the presence of a third base in a second solvent. The 5 third base may be any of a variety of bases which facilitates the desired reaction. The third base may be organic or inorganic. The third base may be selected from the group consisting of sodium hydroxide, potassium hydroxide, lithium hydroxide, sodium carbonate, potassium carbonate, lithium carbonate, triethylamine, diisopropylethylamine, N,N-dimethlyaniline, N,N-diethylaniline, 10 pyridine, and mixtures thereof. In some embodiments, when G is a hydroxyl group, the reaction of the compound of Formula 2 and the chiral acylating agent may occur in the presence of an acid catalyst in the second solvent. The acid catalyst may be organic or inorganic. The acid catalyst may be selected from the group 15 consisting of sulfuric acid, hydrogen chloride, p-toluenesulfonic acid, benzenesulfonic acid, and mixtures thereof. The second solvent may be selected from the group consisting of C1-C10 alkyl ethers (e.g. diethyl ether, methyl t-butyl ether, diisopropyl ether, butyl ether), C1-C1o alkyl esters (e.g. ethyl acetate), C1-C10 ketones (e.g. 20 acetone, methyl ethyl ketone, methyl isobutyl ketone), aromatic C6-C12 hydrocarbons and aliphatic C1-C1o hydrocarbons (e.g. toluene, xylenes, hexanes and heptanes), C 1

-C

10 nitriles, (e.g. acetonitile, propionitrile, butyronitrile, and benzonitrile) C 1

-C

12 N,N-dialkylamides (e.g. N,N-dimethylformamide, N,N-dimethylacetamide and 25 N-methyl-2-pyrrolidinone), C1-C10 sulfoxides and C1-C1o sulfones (e.g. dimethyl sulfoxide and sulfolane), halogenated CI-C10 hydrocarbons (e.g.dichloromethane and dichloroethane), water and mixtures thereof. The second solvent may be dichloromethane. The metal may be a salt of a divalent cation, such as Zn2+ or Mg2+, or a 30 monovalent cation, such as Li*, Na* or K*. The cyclopropyl acetylide metal reagent may be prepared in situ from the reaction of cyclopropyl acetylene and an organometallic compound. The organometallic compound may be WO 2011/009203 PCT/CA2010/001124 23 selected from the group consisting of organomagnesium, organozinc, organosodium, organolithium compounds and mixtures thereof. The organometallic compound may be selected from the group consisting of alkylmagnesium halide, alkylzinc halide, alkyllithium, lithium 5 hexaalkyldisilazide, sodium hexaalkyldisilazide, and potassium hexaalkyldisilazide. The organometallic compound may be selected from the group consisting of ethylmagnesium chloride, methylmagnesium chloride, n-butyllithium, sec-butyllithium, tert-butyllithium, n-hexyllithium and lithium hexamelthyldisilazide. 10 The reaction of a compound of Formula 3 and/or 3a with a compound of Formula 4 may be done in the presence of a third solvent. The third solvent may be an aprotic solvent. The third solvent may be an CI-C1o alkyl ether. The third solvent may be tetrahydrofuran. The reaction may be done at a temperature range between 15 about -78'C to about 30 0 C, In some embodiments, reaction of a compound of Formula 3 and/or 3a with a compound of Formula 4 may give a mixture of two diastereomers of the Formula 5 and 5a: F3C / C1 OH F3C,,,,O 0 OH I-K R 5&.. O NH R 5 5a 20 wherein a desired isomer of the compound of Formula 5 is a major product and R 1 is a chiral auxiliary group. The ratio between the 5 (S) and 5a (R) isomers may range from about 1.1:1 to >99:1. The desired isomer can then be isolated using typical separation methods including selective crystallization. 25 According to illustrative embodiments of the present invention there is provided a process for the preparation of the compound of Formula 1: WO 2011/009203 PCT/CA2010/001124 24 F 3 C K'ox CI (S)Q H 1 the process comprising: i) reacting a compound of Formula 2: 0 C1

CF

3

NH

2 5 2 or an acid addition salt thereof, with a chiral acylating agent of Formula

R

2 COG to form a compound of Formula 3, 3a or mixtures thereof: 0 0 CI R CI R C1CF 3 C1CF 3 0

-KR

2 N R 2 H 3 R 2 O 3a ii) reacting the compound of Formula 3, 3a or mixtures thereof with 10 a compound of Formula 4: M 4 to give a compound of Formula 5:

F

3 C C1 OH N'kR H 5 WO 2011/009203 PCT/CA2010/001124 25 iii) hydrolysing the compound of Formula 5 to form a compound of Formula 8:

F

3 C C1 OH

NH

2 8 ;and; iv) cyclizing the compound of Formula 8 to give the compound of 5 Formula 1, wherein

R

1 is a chiral auxiliary group;

R

2 is a chiral auxiliary group; G is a hydroxyl group, or a leaving group; and 10 M is a metal. In some embodiments of Formula 3 and/or 3a, R 2 is Q of a chiral acid QCOOH, wherein QCOOH is selected from natural chiral organic acids, natural chiral organic acid derivatives, unnatural chiral organic acids, unnatural chiral organic acid derivatives, natural amino acid derivatives, and 15 unnatural amino acid derivatives. In some embodiments of Formula 3 and/or 3a, R 2 is Q of a chiral acid QCOOH, wherein QCOOH is a chiral acid selected from the group consisting of camphanic acid, 2-pyrrolidone-5-carboxylic acid, naproxen, ibuprofen; tartaric acid, malic acid, lactic acid, 3-hydroxybutyric acid, mandelic acid or derivatives thereof. In other embodiments of Formula 20 3, the chiral auxiliary group is a chiral alkoxyl and chiral aralkoxyl groups such as the menthoxy, and camphanoxy groups. In some embodiments of Formula 3 and/or 3a, R 2 is one of the following two groups:

H

3 C CH 3 O'R4

H

3 C C WO 2011/009203 PCT/CA2010/001124 26 wherein R 4 is a hydroxyl protecting group, and the carbon centre designated is enantiomerically enriched in a (R)- or (S)-configuration. R of the compound of Formula 5 is necessarily related to the R2 group of the precursor compound of Formulas 3, 3a or mixtures thereof. Generally, 5 R and R2 will be the same for any given reaction of this sort. Hence, R is as defined for R2 in reactions where the compound of Formula 5 is generated from the compound of Formula 3, 3a or mixtures thereof. The leaving group G may be independently selected from the group consisting of: halogen atoms (e.g. chlorine, bromine and iodine) and 10 sulfonyloxy groups (e.g. methanesulfonyloxy, trifluoromethanesulfonyloxy, p-toluenesulfonyloxy). In some embodiments, G is chlorine. The chiral acylating agent of Formula R 2 COG may be (-)-camphanic acid, (-)-camphanoyl chloride or (R)-2-chloro-2-oxo-1-phenethyl pivalate. The reaction of a compound of Formula 2 and a chiral acylating agent 15 may occur in the presence of a third base or an acid catalyst and typically in the second solvent as described above. The metal may be a salt of a divalent cation, such as Zn 2 + or Mg 2 +, or a monovalent cation, such as Li', Na* or K*. The cyclopropyl acetylide metal reagent may be prepared in situ from the reaction of cyclopropyl acetylene 20 and an organometallic compound. The organometallic compound may be selected from the group consisting of organomagnesium, organozinc, organosodium, organolithium compounds and mixtures thereof. The organometallic compound may be selected from the group consisting of alkylmagnesium halide, alkylzinc halide, alkyllithium, lithium 25 hexaalkyldisilazide, sodium hexaalkyldisilazide, and potassium hexaalkyldisilazide. The organometallic compound may be selected from the group consisting of ethylmagnesium chloride, methylmagnesium chloride, n-butyllithium, sec-butyllithium, tert-butyllithium, n-hexyllithium and lithium hexamelthyldisilazide. 30 The reaction of the compound of Formula 3 and/or 3a with the compound of Formula 4 may be done in the presence of a third solvent. The third solvent may be an aprotic solvent. The third solvent may be an alkyl ether. The third solvent may be tetrahydrofuran.

WO 2011/009203 PCT/CA2010/001124 27 The reaction may be done at a temperature range between about -78*C to about 300C. Hydrolysis of the compound of Formula 5 to the compound of Formula 8 may be performed under acidic or basic conditions. 5 The cyclization of the compound of Formula 8 to give the compound of Formula 1 may be performed with a cyclization reagent. The cyclization reagent may be selected from the group consisting of alkyl haloformates, aryl haloformates, phosgene, triphosgene and 1,1'-carbonyldiimidazole. 10 Examples The following examples are illustrative of some of the embodiments of the invention described herein. These examples should not be considered to limit the spirit or scope of the invention in any way. 15 Example 1: Preparation of 1-(2-amino-5-chlorophenyl)-2,2,2-trifluoroethanone (2): A solution of 1 -(2-amino-5-chlorophenyl)-2,2,2-trifluoroethanone hydrochloride hydrate (48.5 g, 174.4 mmol), 300 mL toluene, and 150 mL water was stirred at room temperature for 30 minutes. The pH of the solution was adjusted to 20 7-8 via the addition of saturated aqueous NaHCO 3 solution. The resulting mixture was then separated, and the toluene layer was collected and evaporated to dryness to give 38.2 g 1-(2-amino-5-chlorophenyl)-2,2,2-trifluoroethanone (2) as a yellow solid. 25 Example 2: Preparation of (iS,4R)-N-(4-chloro-2-(2,2,2-trifluoroacetyl)phenyl) -4,7,7-trimethyl-3-oxo-2-ox abicyclo[2.2.1]heptane-1-carboxamide (9): A solution of 1-(2-amino-5-chlorophenyl)-2,2,2-trifluoroethanone (2, 5.0 g, 22.4 mmol), 30 (-)-camphanoyl chloride (7.35 g, 33.9 mmol), and 50 mL dichloromethane was stirred at room temperature. Triethylamine (1.0 mL, 7.19 mmol, 0.3 eq) was added dropwise. The reaction mixture was stirred overnight at room temperature. The resulting suspension was treated with 10% critic acid aqueous, washed with water and brine. The organic layer was separated and WO 2011/009203 PCT/CA2010/001124 28 evaporated to dryness to give 8.05 g (1S,4R)-N-(4-chloro-2-(2,2,2-trifluoroacetyl)phenyl) -4,7,7-trimethyl-3-oxo-2-ox abicyclo[2.2.1]heptane-1-carboxamide (9). 1 H NMR (CDCl 3 ) 6 11.48 (s, 1H), 8.81 (d, J = 9.1 Hz, 1H), 7.94 (s, 1H), 7.68 (ad, J = 9.1 Hz, 1H), 2.64-2.54 5 (m, 1H), 2.09-1.96 (m, 2H), 1.82-1.73 (m, 1H), 1.17 (s, 3H), 1.15 (s, 3H), 0.99 (s, 3H). Example 3: Preparation of (R)-2-chloro-2-oxo-1-phenylethyl pivalate: (R)-Mandelic 10 acid (10 g, 65.7 mmol) was dissolved in toluene (20 mL) at which point pivaloyl chloride (10.2 mL, 82.9 mmol) was added. This mixture was stirred at 60*C for 3 hours. After cooling the reaction mixture to 300C, toluene (10 mL), N,N-dimethylformamide (0.3 mL) and thionyl chloride (10 mL) were added. The reaction mixture was stirred at 350C for 2 hours and then at 50 0 C for 4 15 hours. Then the mixture was evaporated to dryness to furnish 14.5 g (R)-2-chloro-2-oxo-1-phenylethyl pivalate as a yellow oil. Example 4: Preparation of 20 (R)-2-(4-chloro-2-(2,2,2-trifluoroacetyl)-phenylamino)-2-oxo-1 -phenylethyl pivalate (12): 1-(2-amino-5-chlorophenyl)-2,2,2-trifluoroethanone (2, 5.0 g, 22.4 mmol) was dissolved in dichloromethane (50 mL) and (R)-2-chloro-2-oxo-1-phenylethyl pivalate (8.54 g, 33.5 mmol) was added. To this mixture N,N-dimethylaniline (2.8 mL, 22.4 mmol) was added. The reaction 25 mixture was stirred at room temperature for 3 hours. The mixture was diluted with dichloromethane (50 mL), treated with 1N HCI, washed with brine and the organic layer was separated and evaporated to give (R)-2-(4-chloro-2-(2,2,2-trifluoroacetyl)-phenylamino)-2-oxo-1 -phenylethyl pivalate (12) as an brown oil. 30 Example 5: Preparation of (1 S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1, 1,1 -trifluoro-2-hydroxybut 3-yn-2-yl)phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1 ]heptane-1 -carboxa WO 2011/009203 PCT/CA2010/001124 29 mide (10): Ethylmagnesium bromide 3M in ether (5.98 mL, 17.95 mmol) was added slowly to a solution of cyclopropyl acetylene (1.19 g, 17.95 mmol) in tetrahydrofuran (18 mL) in an ice-bath under argon and the mixture was stirred at 400C for 3 hours. Then (1S,4R)-N-(4-chloro-2-(2,2,2-trifluoroacetyl) 5 phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carboxamide (9, 1.45 g, 3.59 mmol) was added in portions while the flask was in an ice-bath. The reaction mixture was stirred at room temperature for 2 hours. The reaction was quenched with saturated aqueous NH 4 CI, and the mixture was extracted with ethyl acetate and two layers were separated. The organic layer 10 was evaporated to dryness to give 1.67 g of the compound as a crude oil. It was further crystallized with petroleum ether and ethyl acetate to furnish (1S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut 3-yn-2-yl)phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carboxa mide (10) as a white or off-white solid. This solid was shown to be 98.8% de 15 by HPLC. 'H NMR (CDCl 3 ) 6 10.52 (s, 1H), 8.42 (d, J = 8.9 Hz, 1H), 7.72 (s, 1H), 7.35 (ad, J = 8.9 Hz, 1H), 5.05 (s, 1H), 2.68-2.56 (m, 1H), 2.03-1.93 (m, 2H), 1.74-1.65 (m, 1H), 1.44-1.35 (m, 1H), 1.14 (s, 3H), 1.13 (s, 3H), 0.95 (s, 3H), 0.90-0.87 (m, 2H), 0.80-0.77 (m, 2H). 20 Example 6: Preparation of (1S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut 3-yn-2-yl)phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carboxa 25 mide (10): 2.5 M n-Butyllithium (14.8mL, 37.16 mmol) was added slowly to a solution of cyclopropyl acetylene (2.46 g, 37.16 mmol) in tetrahydrofuran (20 mL) in an ice-salt-bath under argon, and then (1S,4R)-N-(4-chloro-2-(2,2,2-trifluoroacetyl)phenyl)-4,7,7-trimethyl-3-oxo-2-ox abicyclo [2.2.1] heptane-1-carboxamide(9, 5 g, 12.39 mmol) was added. The 30 reaction mixture was stirred for 0.5 hour. The reaction was quenched with 10% citric acid aqueous, the mixture was extracted with ethyl acetate and the two layers were separated. The organic layer was evaporated to dryness to give crude oil which was further crystallized with ethyl acetate and petroleum ether to give WO 2011/009203 PCT/CA2010/001124 30 (1 S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1 -trifluoro-2-hydroxybut-3-yn-2-yl )phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo [2.2.1]heptane-1-carboxamide (10) as a white or off-white powder. 1 H NMR spectrum of the product was identical to that of Example 5. 5 Example 7: Preparation of (1 S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1 -trifluoro-2-hydroxybut 3-yn-2-yl)phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1 ]heptane-1 -carboxa 10 mide (10): 1M LiHMDS (7.4 mL, 7.4 mmol) was added slowly to a solution of cyclopropyl acetylene (0.49 g, 7.4 mmol) in THF (4 mL) in an ice-salt bath under argon, and then (1S, 4R)-N-(4-chloro-2-(2,2,2-trifluoroacetyl)phenyl)-4,7,7-trimethyl-3-oxo-2-oxabic yclo[2.2.1]heptane-1-carboxamide (9, 1 g,.2.48 mmol) in THF (1.5 mL) was 15 added slowly. The mixture was stirred at -150C for 20 minutes. TLC showed the formation of the product. The reaction mixture was worked up similarly to the example 6, and (1S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut-3-yn-2-yl )phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2. 1 ]heptane-1 -carboxamide (10) 20 was isolated as an off-white solid. 1 H NMR spectrum of the product was identical to that of Example 5. Example 8: Preparation of 25 (R)-2-(4-chloro-2-((S)-4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut-3-yn-2-yl)phe nylamino)-2-oxo-1-phenylethyl pivalate (13): Ethylmagnesium bromide 3 M in ether (2 mL, 6.2 mmol) was added slowly to a solution of cyclopropyl acetylene (0.41 g, 6.2 mmol) in THF (5 mL) in an ice-bath under argon. The mixture was stirred at 0*C for 1 hour and then 40*C for 2 hours. Then 30 (R)-2-(4-chloro-2-(2,2,2-trifluoroacetyl)phenylamino)-2-oxo-1 -phenylethyl pivalate (12, 0.55 g, 1.24 mmol) in THF (2 mL) was added slowly in an ice-bath. The mixture was stirred at 0*C for 1.5 hours. 10% Citric acid was added to quench the reaction. The organic layer was washed with water and then evaporated to dryness to give WO 2011/009203 PCT/CA2010/001124 31 (R)-2-(4-chloro-2-((S)-4-cyclopropyl-1,1,1 -trifluoro-2-hydroxybut-3-yn-2-yl)phe nylamino)-2-oxo-1-phenylethyl pivalate (13). This solid was shown to be 35.9% de by chiral HPLC. The pure product was isolated by chromatography. 5 Example 9: Preparation of 6-chloro-4-(cyclopropylethynyl)-4-(trifluoromethyl)-1 H-benzo[d] [1,3]oxazin-2(4H)-one (racemic Efavirenz): A solution of N-(4-chloro-2-(4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut-3-yn-2-yl)phenyl) 10 acetamide (5, R 1 is methyl, 1.1 g, 3.3 mmol), toluene (5.5 mL) and triethylamine (0.5 mL, 3.65 mmol) was stirred at 0-5 0 C under nitrogen then 1-chloroethyl chloroformate (0.52 g, 3.65 mmol) was added dropwise. The mixture was stirred at 5-1 0*C overnight. Water (20 mL) was added and the mixture was separated. The organic layer was evaporated to dryness 15 whereupon N, N-dimethyi formamide (5 mL) and K 2

CO

3 (0.9 g, 6.6 mmol) were added. The reaction mixture was stirred at room temperature for 1 day. Toluene (20 mL) and water (20 mL) were added to the mixture, and the resulting mixture was separated. The organic layer was washed with water (20 mL), and evaporated to dryness to give 20 6-chloro-4-(cyclopropylethynyl)-4-(trifluoromethyl)-1 H-benzo[d] [1,3]oxazin-2(4H)-one as an off-white solid. 1H NMR (CDCla) 5 9.64 (s, 1H), 7.49 (a, s, 1H), 7.36 (dd, J = 8.4, 1.8 Hz, 1H), 6.87 (d, J = 8.4 Hz, IH), 1.43-1.36 (m, 1H), 0.97-0.91 (m, 2H), 0.88-0.86 (m, 2H), 25 Example 10: Preparation of (S)-6-chloro-4-(cyclo propylethynyl)-4-(trifluoromethyl)-1 H-benzo[d][1,3]oxazin-2(4H)-one (Efavirenz, 1): A solution of (1S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1 -trifluoro-2-hydroxybut-3-yn-2-yl 30 )phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1 ]heptane-1 -carboxamide (10, 5.0 g, 10.6 mmol), methyl tert-butyl ether (30 mL), 1 N NaOH aqueous (25 mL), and 4-nitrophenyl chloroformate (2.8 g, 13.8 mmol) was stirred at room temperature for 10 minutes. Then two layers were separated and NaOH (2.1 g, 52.5 mmol) was added to the organic layer. The mixture was stirred for 1 WO 2011/009203 PCT/CA2010/001124 32 hour. The suspension was filtered and rinsed with methyl tert-butyl ether (3 X 10 mL), then the filtrate was washed with I N NaOH (25 mL), water (25 mL), and the two layers were separated. The organic layer was evaporated to about 10 mL, heptanes (25 mL) was added. Then the mixture was stirred at 5 room temperature for an hour, the resulting suspension was filtered and washed with heptanes to give 1.5 g (S)-6-chloro-4-(cyclopropylethynyl)-4-(trifluoromethyl)-1,4-dihydro-2H-3,1-ben zoxazin-2-one (Efavirenz, 1) as a white or off-white solid. 10 Example 11: Preparation of (S)-2-(5-chloro-2-((1S, 4R)-4,7,7-trimethyl-3-oxo-2-oxabicyclo [2.2.1 ]heptane-1 -carboxamido)phenyl)-4-cyclopropyl-1 1,1 -trifluorobut-3-yn-2 yl 1-chloroethyl carbonate (7, R 1 is 15 4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptyl,

R

3 is 1-chloroethyl): A solution of (1S, 4 R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut-3-yn-2-yl )phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1] heptane-1-carboxamide (10, 20 g, 42.6 mmol), dichloromethane (100 mL) and triethylamine (5.17 g, 20 51.1 mmol) was stirred at 0-5*C for 20 minutes, then 1-chloroethyl chloroformate (7.3 g, 51.1 mmol) was added dropwise. The mixture was stirred at 5-1 0*C for 1 hour. Water (60ml) was added with stirring and then the layers were separated. The organic solution was concentrated and MTBE (1 80ml) was added. After washing with water twice, the system was 25 concentrated to 30ml and then MTBE (1 Oml) and hexane (60ml) were added. The suspension was stirred at 700C for 30min and filtered to give (S)-2-(5-chloro-2-((1 S,4R)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2. 1 ]heptane- 1 carboxamido)phenyl)-4-cyclopropyl-1,1,1-trifluorobut-3-yn-2-yl 1-chloroethyl carbonate (7, R' is 4

,

7

,

7 -trimethyl-3-oxo-2-oxabicyclo[2.2.1jheptyl,

R

3 is 30 1-chloroethyl) as a white solid. 1 H NMR (CDCl 3 ) 69.43, 9.30 (s, 1H), 8.17, 8.06 (d, J=8.7Hz, 1H), 7.62, 7.54 (s, 1H), 7.42 (d, J=8.7Hz, 1H), 6.36 (q, J=5.7Hz, 1H), 2.60-2.50 (m, 1H), 2.09-1.93 (m, 2H), 1.89-1.86 (at, J=4.5Hz, 3H), 1.77-1.61 (m, 2H), 1.16 (d, J=10.5Hz, 6H), 1.01 (d, J=7.5Hz, 3H), 0.97-0.92 (m, 2H), 0.88-0.84 (m, 2H).

WO 2011/009203 PCT/CA2010/001124 33 Example 12: Preparation of (S)-6-chloro-4-(cyclopropylethynyl)-4-(trifluoromethyl)-1,4-dihydro-2H-3,1-ben 5 zoxazin-2-one (Efavirenz, 1): K 2

CO

3 (13.3 g, 95.8 mmol) powder was added to a solution of (S)-2-(5-chloro-2-((1 S,4R)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1 ]heptane-1 carboxamido) phenyl)-4-cyclopropyl-1,1,1-trifluorobut-3-yn-2-yl 1-chloroethyl carbonate (7, R 1 is 4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptyl, R3 is 10 1-chloroethyl, 36.8 g, 63.9 mmol) in N,N-dimethylformamide (150 mL). The reaction mixture was stirred at room temperature overnight. Methyl t-butyl ether (300 mL) and water (300 mL) were added to the mixture, and the resulting layers were separated. The organic layer was washed with brine and evaporated to 60 mL then petroleum ether (200 mL) was added. The resulting 15 mixture was filtered and rinsed with petroleum ether to give (S)-6-chloro-4-(cyclopropylethynyl)-4-(trifluoromethyl)-1,4-dihydro-2H-3,1-ben zoxazin-2-one (Efavirenz, 1) as an off-white solid. Example 13: 20 Preparation of (S)-6-chloro-4-(cyclo propylethynyl)-4-(trifluoromethyl)-1 H-benzo[d][1,3]oxazin-2(4H)-one (Efavirenz, 1): A solution of (IS,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1 -trifluoro-2-hydroxybut-3-yn-2-y ) phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1]heptane-1-carboxamide 25 (10, 30 g, 63.9 mmol), dichloromethane (150 mL) and triethylamine (7.11 g, 70.2 mmol) was stirred at 0-5 0 C for 20 minutes, then 1-chloroethyl chloroformate (10.0 g, 70.2 mmol) was added dropwise. The mixture was stirred at 5-10*C for 1 hour. Then N,N-dimethylformamide (150 mL) was added and further evaporated to 220 mL, then K 2

CO

3 (13.3 g, 95.8 mmol) was 30 added. The reaction mixture was stirred at room temperature overnight. Methyl t-butyl ether (300 mL) and water (200 mL) were added to the mixture, and the resulting layers were separated. The organic layer was washed with brine, and concentrated to 60 mL whereupon petroleum ether (200 mL) was added and stirred. The resulting precipitate was filtered and rinsed with WO 2011/009203 PCT/CA2010/001124 34 petroleum ether to provide 18.4 g (S)-6-chloro-4-(cyclopropylethynyl)-4-(trifluoromethyl)-1,4-dihydro-2H-3,1-ben zoxazin-2-one (Efavirenz, 1) as an off-white solid. 5 Example 14: Preparation of (S)-4-chloro-2-(4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut-3-yn-2-yl) aniline (8): A suspension of (1S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut-3-yn-2-y 10 )phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2. 1 ]heptane-1 -carboxamide (10, 1 g, 2.13 mmol) and 40% NaOH aqueous solution (20 mL) was stirred at 90-95*C for 20 hours. Toluene (20 mL) was added and the layers were separated. The organic layer was washed with water (20 mL) and evaporated to dryness to give 15 (S)-4-chloro-2-(4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut-3-yn-2-y) aniline (8). 1 H NMR (CDCl 3 ) 6 7.54 (s, 1H), 7.12 (dd, J = 8.5, 1.5 Hz, 1H), 6.62 (d, J = 8.6 Hz, 1H), 4.47 (br. s, 3H), 1.44-1.35 (m, 1H), 0.93-0.88 (m, 2H), 0.83-0.80 (m, 2H). 20 Example 15: Preparation of (S)-6-chloro-4-(cyclopropylethynyl)-4-(trifluoromethyl)-1,4-dihydro-2H-3, 1-ben zoxazin-2-one (Efavirenz, 1): A solution of (S)-4-chloro-2-(4-cyclopropyl-1,1,1 -trifluoro-2-hydroxybut-3-yn-2-yl)aniline 25 (4.42 g, 15.3 mmol), 1,1'-carbonyl diimidazole and toluene (50 mL) was stirred at room temperature for 10 minutes. The reaction was quenched with ice-water (10 mL). The two layers were separated, ethyl acetate (10 mL) was added to the toluene layer and the mixture was washed with brine (2 X 30 mL) and the two layers were separated. The organic layer was evaporated to 30 dryness to give an orange oil and further crystallized with hexanes to give (S)-6-chloro-4-(cyclopropylethynyl)-4-(trifluoromethyl)-1,4-dihydro-2H-3,1-ben zoxazin-2-one as a white or off-white solid. 1 H NMR (CDCl 3 ) 6 9.64 (s, 1 H), 7.49 (a, s, 1H), 7.36 (dd, J = 8.4, 1.8 Hz, 1H), 6.87 (d, J = 8.4 Hz, 1H), 1.43-1.36 (m, 1H), 0.97-0.91 (m, 2H), 0.88-0.86 (m, 2H).

WO 2011/009203 PCT/CA2010/001124 35 Example 16: Preparation of N,N-bis((1S,4R)-4,7,7-trimethyl-3-oxo-2-oxabicyclo [2.2.1 ]heptane-1 -carbonyl)-4-chloro-2-(2,2,2-trifluoroacetyl)aniline (11): A 5 solution of 1-(2-amino-5-chlorophenyl)-2,2,2-trifluoroethanone (2, 40 g, 0.179 mol), (-)-camphanoyl chloride (116 g, 0.537 mol), toluene (120 mL), and dichloromethane (100 mL) was stirred at room temperature. Triethylamine (125 mL, 0.895 mol, 5 eq) was added dropwise. The reaction mixture was stirred at room temperature for 1 day. The resulting suspension was treated 10 with 1 N HCI and washed with water and brine. The organic layer was separated and evaporated to dryness to provide N,N-bis((1 S,4R)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1 ]heptane-1 -carbonyl) 4-chloro-2-(2,2,2-trifluoroacetyl)aniline (11). 15 Example 17: Preparation of (1S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut-3-yn-2-y )phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo [2.2.1]heptane-1-carboxamide (10): 2.5M n-Butyllithium (2.05 mL, 5.14 mmol) was added slowly to a 20 solution of cyclopropyl acetylene (0.34 g, 5.14 mmol) in tetrahydrofuran (4 mL) in an ice-salt-bath under argon, and then N,N-bis((1 S,4R)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1 ]heptane-1 -carbonyl) 4-chloro-2-(2,2,2-trifluoroacetyl)aniline (11, 1 g, 1.71 mmol) in THF (2 mL) was added. The reaction mixture was stirred for 1 hour. The reaction was 25 quenched with 10% citric acid aqueous, the mixture was extracted with ethyl acetate and the two layers were separated. The organic layer was evaporated to dryness to give a crude oil and further crystallized with ethyl acetate and petroleum ether to give pure (1S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1 -trifluoro-2-hydroxybut-3-yn-2-y 30 )phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo [2.2.1]heptane-1-carboxamide (10). Although various embodiments of the invention are disclosed herein, many adaptations and modifications may be made within the scope of the WO 2011/009203 PCT/CA2010/001124 36 invention in accordance with the common general knowledge of those skilled in this art. Such modifications include the substitution of known equivalents for any aspect of the invention in order to achieve the same result in substantially the same way. Numeric ranges are inclusive of the numbers defining the 5 range. The word "comprising" is used herein as an open-ended term, substantially equivalent to the phrase "including, but not limited to", and the word "comprises" has a corresponding meaning. As used herein, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to "a thing" includes more 10 than one such thing. Citation of references herein is not an admission that such references are prior art to the present invention. Any priority document(s) are incorporated herein by reference as if each individual priority document were specifically and individually indicated to be incorporated by reference herein and as though fully set forth herein. The invention includes 15 all embodiments and variations substantially as hereinbefore described and with reference to the examples and drawings.

Claims

1. A process for the preparation of a compound of Formula 1 F 3 C "s(S)Q H 5 1 the process comprising cyclizing, in the presence of a first base, a compound of Formula 5: F 3 C CO OH 0 N"K R' H 5 with a haloformate of Formula 6: 0 10 6 wherein R 1 is alkyl, substituted alkyl, aryl, substituted aryl, arylalkyl, substituted arylalkyl or a chiral auxiliary group; R is alkyl, substituted alkyl, aryl, substituted aryl, arylalkyl, or substituted 15 arylalkyl; and X is halogen.

2. The process of claim 1 further comprising treatment with a second base. 20

3. The process of claim 2 wherein the second base is selected from the group consisting of sodium hydroxide, potassium hydroxide, lithium hydroxide, WO 2011/009203 PCT/CA2010/001124 38 sodium carbonate, potassium carbonate, lithium carbonate, triethylamine diisopropylethylamine, N,N-dimethylaniline, and N,N-diethylaniline, pyridine and mixtures thereof. 5

4. The process of claim 3 wherein R' is selected from the group consisting of methyl, ethyl, isobutyl, tert-butyl, and benzyl.

5. The process of claim 3 wherein R' is a chiral auxiliary group. 10

6. The process of claim 5 wherein the compound of Formula 5 is (1S,4R)-N-(4-chloro-2-((S)-4-cyclopropyl-1,1,1-trifluoro-2-hydroxybut-3-yn-2-y )phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1 ]heptane-1 -carboxamide.

7. The process of claim 5 wherein the compound of Formula 5 is 15 (R)-2-(4-chloro-2-((S)-4-cyclopropyl-1,1,1 -trifluoro-2-hydroxybut-3-yn-2-yl)phe nylamino)-2-oxo-1-phenylethyl pivalate.

8. The process of claim 5 wherein the haloformate of Formula 6 is selected from the group consisting of 4-nitrophenyl haloformate, 4-chlorophenyl 20 haloformate, phenyl haloformate and 1-chloroethyl haloformate.

9. The process of claim 5 wherein the first base is selected from the group consisting of sodium hydroxide, potassium hydroxide, lithium hydroxide, sodium carbonate, potassium carbonate, lithium carbonate, triethylamine 25 diisopropylethylamine, N,N-dimethylaniline, and N,N-diethylaniline, pyridine and mixtures thereof.

10. The process of claim 2 wherein an intermediate of Formula 7: WO 2011/009203 PCT/CA2010/001124 39 F 3 C 0 OR 3 0 N R1 H 7 is isolated before treatment with the second base, wherein R' is alkyl, substituted alkyl, aryl, substituted aryl, arylalkyl, substituted 5 arylalkyl or a chiral auxiliary group; and R 3 is alkyl, substituted alkyl, aryl, substituted aryl, arylalkyl, or substituted arylalkyl.

11. The process of claim 10 wherein the second base is selected from the 10 group consisting of sodium hydroxide, potassium hydroxide, lithium hydroxide, sodium carbonate, potassium carbonate, lithium carbonate, triethylamine diisopropylethylamine, N,N-dimethylaniline, and N,N-diethylaniline, pyridine and mixtures thereof. 15

12. The process of claim 11 wherein R' is selected from the group consisting of methyl, ethyl, isobutyl, tert-butyl, and benzyl.

13. The process of claim 11 wherein R 1 is a chiral auxiliary group. 20

14. The process of claim 13 wherein the compound of Formula 5 is (1S,4R)-N-(4-chloro-2-((S)-4-cycopropyl-1,1,1-trifluoro-2-hydroxybut-3-yn-2-y )phenyl)-4,7,7-trimethyl-3-oxo-2-oxabicyclo[2.2.1 ]heptane-1 -carboxamide.

15. The process of claim 13 wherein the compound of Formula 5 is 25 (R)-2-(4-chloro-2-((S)-4-cyclopropyl-1,1,1 -trifluoro-2-hydroxybut-3-yn-2-yl)phe nylamino)-2-oxo-1-phenylethyl pivalate. WO 2011/009203 PCT/CA2010/001124 40

16. The process of claim 13 wherein the haloformate of Formula 6 is selected from the group consisting of 4-nitrophenyl haloformate, 4-chlorophenyl haloformate, phenyl haloformate and 1-chloroethyl haloformate. 5

17. The process of claim 13 wherein the first base is selected from the group consisting of sodium hydroxide, potassium hydroxide, lithium hydroxide, sodium carbonate, potassium carbonate, lithium carbonate, triethylamine diisopropylethylamine, N,N-dimethylaniline, and N,N-diethylaniline, pyridine and mixtures thereof. 10

18. A process for making a compound of Formula 5 F 3 C ci OH CN)k R' H 5 the process comprising: i) reacting a compound of Formula 2: 0 Ci CF 3 NH 2 15 2 or an acid addition salt thereof, with a chiral acylating agent of Formula R 2 COG to form a compound of Formula 3, Formula 3a or a mixture thereof: 0 0 C / CF 3 C / CF 3 -I 1 " 10 N R2 Nk R 2 H 3 R 2 O 3a WO 2011/009203 PCT/CA2010/001124 41 ii) reacting the compound of Formula 3, Formula 3a or the mixture thereof with a compound of Formula 4: M 4 to form the compound of Formula 5, 5 wherein R 2 is a chiral auxiliary group; G is a hydroxyl group, or a leaving group; and M is a metal. 10

19. The process of claim 18 wherein G is chloro or the hydroxyl group and R 2 is:

20. The process of claim 18 wherein G is chloro and R 2 is: o o 15

21. The process of claim 18 wherein R 2 is: OR 4 R 4 is a hydroxyl protecting group; and 20 the carbon center designated "*" is enantiomerically enriched in a (R)-or (S)-configuration.

22. The process of claim 18 wherein M is lithium, sodium, potassium, magnesium halide or mixtures thereof. WO 2011/009203 PCT/CA2010/001124 42

23. The process of claim 18 wherein M is lithium.

24. A process for the preparation of a compound of Formula 1: F 3 C O C1 N O 5 H 1 the process comprising: i) reacting a compound of Formula 2: 0 CI CF 3 NH 2 2 10 or an acid addition salt thereof, with a chiral acylating agent of Formula R 2 COG to form a compound of Formula 3, Formula 3a or a mixture thereof: 0 0 C CF 3 C1 / CF 3 I o | o N 2 N R 2 H 3 R 2 O 3a ii) reacting the compound of Formula 3, Formula 3a or the mixture thereof with a compound of Formula 4: ~_ M 15 4 to form a compound of Formula 5: WO 2011/009203 PCT/CA2010/001124 43 F 3 C CR OH 0 H 5 iii) hydrolysing the compound of Formula 5 to form a compound of Formula 8: F 3 C 7 OH NH 2 8 ;and 5 iv) cyclizing the compound of Formula 8 to give the compound of Formula 1, wherein R' and R2 are a chiral auxiliary group; G is a hydroxyl group, or a leaving group; and 10 M is a metal.

25. The process of claim 24 wherein G is chloro or the hydroxyl group and R 1 and R 2 are: 15

26. The process of claim 24 wherein G is chloro and R 1 and R 2 are: WO 2011/009203 PCT/CA2010/001124 44 0 0

27. The process of claim 24 wherein RI and R 2 are: OR 4 5 R 4 is a hydroxyl protecting group; and the carbon centre designated "*" is enantiomerically enriched in a (R)- or (S)-configuration. 10

28. The process of claim 24 wherein M is lithium, sodium, potassium, magnesium halide or a mixture thereof.

29. The process of claim 24 wherein M is lithium. 15

30. The process of claim 24 wherein the cyclization of a compound of Formula 8 to give the compound of Formula 1 is performed with a cyclization reagent selected from the group consisting of C 1 -C 10 alkyl haloformates, C 6 -C 12 aryl haloformates, phosgene, triphosgene and 1,1'-carbonyldiimidazole. 20

31. A compound of Formula 3: 0 C / CF 3 M-0 V N-f- R2 H 3 wherein R 2 is a chiral auxiliary group. WO 2011/009203 PCT/CA2010/001124 45

32. The compound of claim 31 wherein the chiral auxilliary group is Q of a chiral acid QCOOH, wherein QCOOH is selected from the group consisting of: natural chiral organic acids, natural chiral organic acid derivatives, unnatural 5 chiral organic acids, unnatural chiral organic acid derivatives, natural amino acid derivatives, and unnatural amino acid derivatives.

33. The compound of claim 31 wherein the chiral auxiliary group is a chiral C 1 -C 10 alkoxyl or a chiral C 6 -C 12 aralkoxyl group. 10

34. A compound of Formula 3a: 0 Cl/ CF 3 R2- 0 3a wherein R 2 is a chiral auxiliary group. 15

35. The compound of claim 34 wherein the chiral auxilliary group is Q of a chiral acid QCOOH, wherein QCOOH is selected from the group consisting of: natural chiral organic acids, natural chiral organic acid derivatives, unnatural chiral organic acids, unnatural chiral organic acid derivatives, natural amino 20 acid derivatives, and unnatural amino acid derivatives.

36. The compound of claim 34 wherein the chiral auxiliary group is a chiral C 1 -C 10 alkoxyl or a chiral C 6 -C 12 aralkoxyl group. 25

37. A compound of Formula 5: WO 2011/009203 PCT/CA2010/001124 46 F 3 C c1 OH H 5 wherein R 1 is a chiral auxiliary group. 5

38. The compound of claim 37 wherein the chiral auxilliary group is Q of a chiral acid QCOOH, wherein QCOOH is selected from the group consisting of: natural chiral organic acids, natural chiral organic acid derivatives, unnatural chiral organic acids, unnatural chiral organic acid derivatives, natural amino acid derivatives, and unnatural amino acid derivatives. 10

39. The compound of claim 37 wherein the chiral auxiliary group is a chiral C 1 -C 10 alkoxyl or a chiral C6-C2 aralkoxyl group.

40. A compound of Formula 9: 0 C / CF 3 NH 0 15 9

41. A compound of Formula 10: WO 2011/009203 PCT/CA2010/001124 47 F 3 C c1 OH NH 0 0 0 10

42. A compound of Formula 11: 0 Ci CF 3 C 0 00 11 5

43. A compound of Formula 12: 0 C1 HCF 3 NH 0 0 12

44. A compound of Formula 13: WO 2011/009203 PCT/CA2010/001124 48 F 3 C Ci HOH NH Ph O 0 0 13

45. A compound of Formula 14: 0 CF 3 NH Ph O OR 4 14 5 wherein R 4 is a hydroxyl protecting group; and the carbon centre designated "*" is enantiomerically enriched in a (R)- or (S)-configuration. 10

46. A compound of Formula 15: F 3 C Cl OH NH Ph OR 4 15 wherein R 4 is a hydroxyl protecting group; WO 2011/009203 PCT/CA2010/001124 49 and the carbon centre designated "*" is enantiomerically enriched in a (R)- or (S)-configuration.

47. A compound of Formula 7: F 3 C Ck-0 OR 3 5 H wherein R1 is alkyl, substituted alkyl, aryl, substituted aryl, arylalkyl, substituted arylalkyl or a chiral auxiliary group; and R 3 is alkyl, substituted alkyl, aryl, substituted aryl, arylalkyl, or substituted 10 arylalkyl.

48. A composition comprising a compound of Formula 1: C F 3 C (S)Q N 0~ H 1 15 and a compound of Formula 7: F 3 c N R 1 H 7

49. A composition comprising a compound of Formula 1: WO 2011/009203 PCT/CA2010/001124 50 ciF3C 7 NO H 1 and a compound of Formula 5: F 3 C 7/ ci OH 10 H 5 5