AU2009305343A1 - Clarification process at higher cell density - Google Patents

Clarification process at higher cell density Download PDF

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Publication number
AU2009305343A1
AU2009305343A1 AU2009305343A AU2009305343A AU2009305343A1 AU 2009305343 A1 AU2009305343 A1 AU 2009305343A1 AU 2009305343 A AU2009305343 A AU 2009305343A AU 2009305343 A AU2009305343 A AU 2009305343A AU 2009305343 A1 AU2009305343 A1 AU 2009305343A1
Authority
AU
Australia
Prior art keywords
cell
cells
desired biological
broth
supernatant layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2009305343A
Other languages
English (en)
Inventor
Emily Belcher Schirmer
Michael Christopher Kuczewski
Grigorios Zarbis-Papastoitsis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Percivia LLC
Original Assignee
Percivia LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Percivia LLC filed Critical Percivia LLC
Publication of AU2009305343A1 publication Critical patent/AU2009305343A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
AU2009305343A 2008-10-17 2009-10-16 Clarification process at higher cell density Abandoned AU2009305343A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US10632008P 2008-10-17 2008-10-17
US61/106,320 2008-10-17
EP08167506.8 2008-10-24
EP08167506 2008-10-24
PCT/EP2009/063565 WO2010043700A1 (fr) 2008-10-17 2009-10-16 Procédé de clarification à une densité de cellules supérieure

Publications (1)

Publication Number Publication Date
AU2009305343A1 true AU2009305343A1 (en) 2010-04-22

Family

ID=40367660

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2009305343A Abandoned AU2009305343A1 (en) 2008-10-17 2009-10-16 Clarification process at higher cell density

Country Status (7)

Country Link
EP (1) EP2346891A1 (fr)
JP (1) JP2012505863A (fr)
KR (1) KR20110085980A (fr)
CN (1) CN102186875A (fr)
AU (1) AU2009305343A1 (fr)
CA (1) CA2739501A1 (fr)
WO (1) WO2010043700A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012030512A1 (fr) 2010-09-03 2012-03-08 Percivia Llc. Procédé de purification de protéine en flux
AU2014326706A1 (en) 2013-09-30 2016-03-10 Janssen Vaccines & Prevention B.V. Method for the clarification of high density crude cell culture harvest
SG10201911134QA (en) 2015-06-05 2020-01-30 Grace W R & Co Adsorbent bioprocessing clarification agents and methods of making and using the same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2460819A1 (fr) * 2001-09-18 2003-03-27 Affinium Pharmaceuticals, Inc. Procedes et appareils de purification
CN101426905B (zh) * 2006-04-20 2013-03-27 惠氏公司 从细胞培养物分离纯化的水泡性口炎病毒的纯化方法

Also Published As

Publication number Publication date
CA2739501A1 (fr) 2010-04-22
JP2012505863A (ja) 2012-03-08
CN102186875A (zh) 2011-09-14
EP2346891A1 (fr) 2011-07-27
WO2010043700A1 (fr) 2010-04-22
KR20110085980A (ko) 2011-07-27

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Legal Events

Date Code Title Description
MK4 Application lapsed section 142(2)(d) - no continuation fee paid for the application