AU2007211939A1 - Nanoparticle probes with raman spectroscopic fingerprints for analyte detection - Google Patents

Description

P001 Section 29 Regulation 32(2)

AUSTRALIA

Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT Application Number: Lodged: Invention Title: Nanoparticle probes with raman spectroscopic fingerprints for analyte detection The following statement is a full description of this invention, including the best method of performing it known to us: Nanoparticle Probes with Raman Spectroscopic Fingerprints For Analyte Detection Cross-reference: This application is a continuation-in-part of U.S. patent application No. 10/172,428, filed June 14, 2002 which claims the benefit of priority based on U.S. provisional applications Nos.

60/378,538, filed May 7, 2002 (case no. 02-338); and 60/383,630, filed May 28, 2002 (case no.

02-338-A), all which are hereby incorporated by reference in their entirety. The work described in this application has been supported in part from grants from the Air Force Office of Scientific Research, DARPA, and the NSF. Accordingly, the United States government may have some rights to the invention.

Background of the Invention: The development of high-sensitivity, high-selectivity detection formats for chemical and biological molecules is of paramount importance for realizing the full potential of genomics and proteomics advances made over the past decade.

1 High density gene chips have made it possible to monitor the levels of expression of thousands of genes simultaneously. Lower density chips have shown promise for both laboratory and clinical identification of many potential biohazards in one sample. Although the core accepted and utilized labeling technology is currently based upon molecular fluorophore markers, recent advances in nanoparticle technology have pointed toward systems with significantly higher sensitivities and selectivities and potentially more straightforward and versatile readout hardware than conventional fluorescence-based approaches.

5 1 7 A strong argument is being made for nanoparticles as the next generation labeling technology for biodiagnostic research.

One of the most sensitive and selective detection formats for DNA relies on oligonucleotide-functionalized nanoparticles as probes, a particle-initiated silver developing technique for signal enhancement, and a flatbed scanner for optical readout.

8 The current demonstrated detection limit for this "scanometric DNA detection" format is 100 aM, and the utility of the system has been demonstrated with short synthetic strands, PCR products, and genomic DNA targets.

1 7 1 8 A limitation of this approach is that it is inherently a one color system based upon grey scale. The flexibility and applicability of all DNA detection systems benefit S from access to multiple types of labels with addressable and individually discernable labeling information. In the case of fluorescence, others have demonstrated that one can use multiple fluorophores, including quantum dots, to prepare encoded structures with optical signatures that j- depend upon the types of fluorophores used and their signal ratio within the probes.", 9 These approaches typically use micron size probes so that they can obtain encoded structures with the S appropriate signal intensities and uniformities. Moreover, in the case of molecular fluorophores, o due to overlapping spectral features and non-uniform fluorophore photobleaching rates,"" this S approach has several potential complications.

The art describes the use of Surface Enhanced Raman Spectroscopy (SERS) to detect various analytes. For example, U.S. Patent 5,306,403 describes a method and apparatus for DNA sequencing using SERS. U.S. Patent 5,266,498 describes the use of SERS to detect analytes in general. U.S. Patent 5,445,972 describes the use of a Raman label bound to a specific binding molecule. U.S. Patent 5,376,556 describes the use of SERS in immunoassays.

U.S. Patent 6,127,120 describes the use of SERS, the detection of nucleic acid and nucleic acid subunits. U.S. Patents 6,242,264 and 6,025,202 describe the use of silver to form a SERS active substrate to enhance Raman scattering of adsorbed molecules. None of the the previous SERSbased detection methodologies were demonstrated for use single or multiplexed sandwich hybridization assay formats. This absence may be due, in part, to the difficulty in reproducibly generating and functionalizating stable SERS-active substrates2 3 as well as lack of an appropriate probe design strategy to enable multiplexed detection. Accordingly, there is a need for probes and methods for use in SERS-based detection assays, particularly in single or multiplexed sandwich hybridization assay formats.

In the present invention provides a novel detectionreagent comprising a particle comprising a Raman label and specific binding member bound to the particle for use in SERSbased assays of analytes. In the presence of a target analyte, a substrate containing a capture probe for the analyte, and the detection reagent, the reagent advantageously complexes or binds to the binding partner analyte to form a complex which directly or indirectly binds to the support. The Raman label in the labeled complex on the support can then be SERS activated by staining, for example, silver, gold or copper enhancement to achieve a SERS effect when irradiated with a laser. Generally this complex is captured on a solid support and treated with silver to provide a SERS effect Alternatively, the complex can be directly or indirectly complexed with an analyte which has already been bound directly or indirectly to a solid support substrate. In the present invention, the SERS effect is produced near the time it is measured.

This reagent can advantageously include multiple different Raman dyes bound to be particle carriers as a way distinguishing particular carriers with particular specific binding members as a way of indexing a vast number of reagent for multiplex application.

C The invention also provides a detection reagent comprising a conjugate of several different Raman dyes bound to a specific binding substance such as DNA, RNA, polypeptide, C antibody, antigen, small molecules, etc. This also serves as a reagent indexing tool.

O The invention is particularly distinguished from the prior art method in that the SERS ,1 technology is used in conjunction with nanoparticle assay techniques to provide extraordinary sensitivity and specificity of detection of analytes which is particularly amenable to multiplexed determination of analytes.

Description of the Drawings: Figure 1 illustrates a chip-based DNA detection method using nanoparticles functionalized with oligonucleotides and Raman labels.

Figure 2 illustrates a flatbed scanner image of microarrays after hybridized with nanoparticles functionalized with Cy3 labels, before and after silver staining. A typical Raman spectrum acquired from one of the silver stained spots. A profile of Raman intensity at 1192 cm 1 as a function of position on the chip; the laser beam from the Raman instrument is moved over the chip from left to right as defined by the line in Figure 3 illustrates the unoptimized detection limit of DNA using the Raman scanning method. A microarray-based sandwich detection format; A flatbed scanner image of microarrays for 20 fM target concentration after hybridized with nanoparticles functionalized with Cy3.5 labels; A typical Raman spectrum acquired from one of the silver-stained spots; A profile of Raman intensity at 1199 cmnf as a function of position on the chip; the laser beam from the Raman instrument is moved over the chip from left to right as defined by the line in Figure 4 illustrate Left: The Raman spectra of six dyes. Each dye correlates with a different color in our labeling scheme (see rectangular boxes). Right: six DNA target analysis systems. The information of target strand sequences were obtained from the web site of the National Center for Biological Information (http://www2.cbimlm.nihgov/Genbalk/index.html).

Figure 5 illustrates Flatbed scanner images of silver-stained microarrays and (B) corresponding Raman spectra. The colored boxes correlate with the color coded Raman spectra in Figure 4.

Figure 6 iUustrates the differentiation of two RNA targets (Target 1: perfect; Target 2: with one-base difference).

Figure 7 illustrates hybridization of pure RNA target 1 or 2, or mixture of target 1 and 2, to microarrays before stringency wash, after stringency wash.

Figure 8 illustrates Typical flatbed scanner images of microarrays hybridized with nanoparticles, before and after stringency wash but prior to silver enhancing, and after silver enhancing. Flatbed scanner image of microarrays hybridized with nanoparticles before stringency wash but aflter silver enhancement A typical Raman spectrum (purple line) of the silver enhanced spots in compared with the spectrum (black line) for mixed probes (1:1, probe 1.-probe 2, after silver enhacement). Raman spectrum of the mixed probes (probe 1 -probe 2, 1:1, after silver enhacement) compared with the spectra for probe 1 (with only TMR, blue line) or probe 2 (with only Cy3, red line).

Figure 9 illustrates typical flatbed scanner images of nanoparticle-functionalized microarrays, befbre and after stringency wash but prior to silver staining, and alter silver staining. Raman spectra (1550 -1750 cm 1 from the stained spots at different ratios of target land target 2: 1:0; 5:1; 3:1; 1:1; 1:2; 1:3; 1:5; 0:1. The full Raman spectra from 400 to 1800 cm 1 are shown in the supporting informatioL The inset is a profile of Raman intensity ratio (12/11) verse target ratio (T 2 where I, is the Raman Intensity at 1650 cm 1 (from probe 1: TMR labeled gold oligonucleotide conjugate); 12 is the Raman Intensity at 1588 cm- 1 (from probe 2: Cy3 labeled gold oligonucleotide conjugate).

Figure 10 illustrates Raman spectra (400 -1800 cm 1 from the silver enhanced spots at different target 1 to target 2 ratios: 1:0; 5:1; 3:1; 1:1; 1:3; 1:5; and 0:1.

Figure 11 illustrates Scheme for screening protein-small molecule interactions. (3) Flatbed scanner images of silver-stained microarrays and corresponding Raman spectra according to the color coded scheme in Figure 4. Biotin was labeled with Cy3, DIG with and DNP with Cy5. See supporting information for probe preparation details.

Figure 12 illustrates the Raman-based detection format for proteins.

Figure 13 illustrates (Al-4) Flatbed scanner images of silver-stained microarrays associated with the protein-protein screening experiments. Color code for the Raman identification of the probes in the silver stained spots; no cross reactivity is observed. Anti- Mouse IgG was labeled with Cy3 modified-alkylthiol-capped poly adenine (A 0 io), anti-ubiquitin by Cy3.5 modified-alkylthiol-capped Poly adenine (A 10 and anti-human protein C by modified-alkylthiol-capped Poly adenine (A1o). The A 10 oligonucleotide spacer was used to enhance the stability of the particle probes.

33 Figure 14 illustrates the examples for creating Raman-labeled nanoparticle probes with multiplexing capabilities. R1, R2, are R3 are different Raman dyes.

Figure 15 illustrates the creation of massive nanoparticle probes with multiple Raman labels.

Figure 16 illustrates Left: Raman spectrum of a probe with two Raman labels (Cy3:TMR=1 black line) after Ag staining in microarray form compared with the spectra for probes with only TMR (blue line) or Cy3 (red line). Right: Raman spectra of two-dye functionalized nanoparticle probes as a function of Cy3 to TMR ratio.

Figure 17 illustrates Left and Right: two Raman spectra of three-dye composite labels (black line) compared with the spectra of TMR (blue line), Cy3 (red line) and Cy3.5 (green line).

Figure 18 illustrates the microbead-based detection format using the scanning Raman method.

Figure 19 illustrates and The eight DNA target analysis systems. Each of the probe strands was marked by a single-dye or two-dye labels (see rectangular boxes and circles, corresponding Raman spectra. The colored boxes and circles correlate with the color coded Raman spectra in Figure Figure 20 illustrates the Raman spectra of six single dyes and two mixed dyes, each spectra correlates with a different color in our labeling scheme (see rectangular boxes and circles).

Figure 21 illustrates microscopy image of silver-stained microspheres. The colored 0 circles correlate with the color coded Raman spectra in Figure Figure 22 illustrates optical microscope image of aligned silver-stained microspheres.

SThe colored boxes correlate with the color coded Raman spectra in Figure Figure 23 illustrates the fiber optic-based detection format using microbeads.

Figure 24 illustrates the synthesis of Raman labeled oligonucleotides.

Figure 25 illustrates Raman detection experiments on polymer substrates. A: Silver S stained Cy3 modified BNT A2o gold nanoparticle probes on nitrocellulose; B: Silver stained modified DIG A 20 gold nanoparticle probes on PVDF.

Figure 26 is a schematic for using Raman labeling technique in a Western blotting S experiment.

Figure 27 illustrates: A Cy3.5-labeled gold nanoparticle modified with anti-ubiquitin for use as an ubiquitin probe. The gold-particle-probe-developed nitrocellulose membrane (after silver staining). A typical Raman spectrum from the dark gray area with silver-stained gold nanoparticles.

Summary of the invention: The present invention relates to SERS-based detection methods, optical device, and detection probes comprising particles or carriers or Raman dye carriers functionalized with specific binding members and Raman labels. The detection probes, coupled with surfaceenhanced Raman scattering (SERS) spectroscopy, can be used to perform multiplexed detection of analytes. This is exemplified for DNA and RNA targets in Figure 1. Although oligonucloetides can be directly detected by SERS on aggregated particles, 26 the structural similarities of oligonucleotides with different sequences results in spectra that are difficult to distinguish. Therefore, one must use different Raman dyes to label different oligonucleotides to distinguish oligonucleotide sequences.

2 0 21 Previously a SERS-based detection methodology that allows for single or multiplexed sandwich hybridization assay formats had not been demonstrated. In part, this conspicuous technological absence is due to the difficulty in reproducibly generating and funetionalizing stable SERS-active substrates 2 3 as well as a lack of an appropriate probe design strategy to enable multiplexed detection. To get the benefits of high sensitivity and high selectivity detection coupled with multiple labeling capabilities, a new type of particle probe has been designed that can be used, for example, for DNA (or RNA) detection (Figure but is equally applicable to other specific binding substances such as proteins, peptides, drugs, small molecules, etc. Preferably, the detection probes comprise gold particles functionalized with Raman-dye labeled oligonucleotides. However, particles of any suitable various size, shape and materials may be used. The Raman spectroscopic fingerprint, which can be designated through choice of Raman label can be read out after silver enhancing via scanning Raman spectroscopy (Figure Other enhancers such as gold or copper staining materials may be used. Because the SERS-active substrate in this strategy is generated prior to the detection event, a large and reproducible Raman scattering response can be obtained.

According, in one embodiment of the invention, detection reagents or probes are provided. In one aspect of this embodiment, the detection reagent comprises particles having specific binding members and Raman labels bound to the particle. The Raman label may be bound directly or indirectly to the particle in any suitable manner including the use of functional groups such as thiols and linkers having functional groups. Alternatively, the Ramnan labels can bound to the the specific binding member and the resulting entity can be attached to the particle.

When utilized in a SERS-detection sandwich-based assay, the detection reagent can be treated with an enhancing stain such as silver, gold or copper to provide a SERS effect when irradiated.

When employed in a detection method-, this reagent may be complexed with analyte which binds to the specific binding member and the resulting complex can be directly or indirectly captured on a substrate. The Ramnan label in the complex on the substrate is treated with a staining agent such as silver, gold or copper to activate the SERS effect when irradiated with a laser. Alternatively, the analyte maybe captured on the solid support substrate directly or indirectly and reacted directly or indirectly with the detection reagent prior to staining and SERS measurement. Two or more different Raman labels may be used on the particle for multiplexing applications.

1n another aspect of this embodiment, invention, a detection reagent is provided which comprises a specific binding substance having two or more different Ramnan labels bound thereto. The use of two or more different Raman labels on a reagent particle or a specific binding substance provides a way of indexing vast numbers of different particles and reagents for multiplexing applications.

In other embodiment of the invention, a method is provided for detecting analytes using these reagents.

Accordingly, in one aspect of this embodiment of the invention, a method for detecting for the presence or absence of one or more target analytes in a sample, the target analytes having at least two binding sites, is provided. The method comprises: providing a substrate having bound thereto one or more types of a first specific binding complements Or capture probes for immobilizing the target analyte directly or indirectly onto said substrate; providing one or more types of detection probes, each type of detection probes comprising particles having bound thereto one or more Raman active labels; and a second specific binding complement for direct or indirect binding to a specific target analyte, wherein (i) the Ramnan active label bound to each type of particle is different and serves as an identifier for a specific target analyte; (ii) the second specific binding complement bound to each type of particle is different and is targeted to a specific target analyte; and (iii) the Raman active label comprises at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering; contacting the particles, the sample and the substrate under conditions effective for specific binding interactions between the target analyte and first and second specific binding complements so as to form a test substrate having particles complexed thereto in the presence of one or more target analytes in the sample; contacting the test substrate with a staining material to produce a detection substrate having a surface capable of causing surface-enhanced Raman scattering (SERS); and determining for the presence of said particle complexes on said detection substrate as an indication of the presence of one or more target analytes in the sample by obtaining and analyzing a SERS spectrum. The first specific binding complements bound to the substrate are capture probes which directly or indirectly immobilize the target analyte to the substrate. The capture probes may be arrayed on the substrate in discrete areas to allow for the detection of one or more target analytes or portions of the target analytes in a sample. In addition, the detection probes can be contacted first with the target analyte under conditions effective for allowing specific binding interactions between the target analyte and the detection probes prior to contacting with the capture probes on the substrate. Alternatively, the target analyte can be contacted first with the capture probes on the substrate under conditions effective to allow for specific binding interactions between the capture probes and the analyte prior to contact with the detection probes. Alternatively, the detection probe, target analyte and capture probe can be contacted simultaneously.

In another aspect of this embodiment,, a method for detecting for the presence or absence of one or more target nucleic acids in a sample, the sequence of the nucleic acid having at least two portions, is provided. The method comprises: providing a subsirate having a oligonucleotides bound thereto, the oligonucleotides bound to the substrate having a sequence that is complementary to a first portion of the nucleic acid; providing one or more types of particles comprising oligonucleotides bound thereto and a Raman active label bound to a portion of the oligonucleotides, wherein at least some of the oligonucleotides attached to each type of particle have a sequence that is complementary to a second portion of the sequence of a specific target nucleic acid; and (ii) the Raman active label bound to each type of particles is different and serves as an identifier for a specific target nucleic acid, said Raman active label comprising at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing Raman scattering; contacting the particles, the substrate, and the sample under conditions effective for hybridization of the oligonucleotides bound to the substrate with the first portion of the nucleic acid and for hybridization of the oligonucleotides attached to the particle with the second portion of the nucleic acid so as to form a test substrate having one or more particle complexes bound thereto when one or more target nucleic acids are present in said sample; contacting the test substrate with a staining material to produce a detection substrate having a surface capable of causing surface-enhanced Raman scattering (SERS); and determining for the presence of said particle complexes on said detection substrate as an indication of the presence of one or more target nucleic acids in the sample by obtaining and analyzing a SERS spectrum.

In yet another embodiment of the invention, a method. for detecting for the presence or absence of a target nucleic acid in a sample, the sequence of the nucleic acid having at least two portions, is provided. The method comprises: providing a substrate having oligonucleotides bound thereto, the oligonucleotides bound to the substrate having a sequence that is complementary to a first portion of the nucleic acid; providing a particle comprising oligonucleotides bound thereto and a Raman label bound to a portion of the oligonucleotides, wherein at least some of the oligonucleotides attached to the particle have a sequence that is complementary to a second portion of the nucleic acid; and (ii) the Raman active label bound to particles serves as an identifier for the target nucleic acid, said Raman active label comprising at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering; contacting the particles, the substrate, and the sample under conditions effective for hybridization of the oligonucleotides bound to the substrate with the first portion of the nucleic acid and for hybridization of the oligonucleotides attached to the particle with the second portion of the nucleic acid so as to form a test substrate having a particle complex bound thereto when said target nucleic acid is present in said sample; contacting the test substrate with a staining material to produce a detection substrate having a surface capable of causing surface-enhanced Raman scattering (SERS); and determining for the presence of said particle complex on said detection substrate as an indication of the presence of the target nucleic acid in the sample by obtaining and analyzing a SERS spectrum.

In yet another embodiment of the invention, a method for detecting for the presence or absence of a single nucleotide polymorphism in a nucleic acid in a sample, the sequence of the nucleic acid having at least two portions, is provided. The method comprises: providing a substrate having a oligonucleotides bound thereto, the oligonucleotides bound to the substrate having a sequence that is complementary to a first portion of the nucleic acid; providing one or more types of particles comprising oligonucleotides bound thereto and a Raman active label bound to a portion of the oligonucleotides, wherein at least some of the oligonucleotides attached to each type of particle have a sequence that is believed to be complementary to a second portion of the sequence of the nucleic acid, said second portion of the sequence of the nucleic acid is suspected of having a single nucleotide substitution when compared to a wild type sequence of the nucleic acid; and (ii) the Raman active label bound to each type of particles is different and serves as an identifier for a specific sequence having a single nucleotide substitution, said Raman active label comprising at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering; contacting the particles, the substrate, and the sample under conditions effective for hybridization of the oligonucleotides bound to the substrate with the first portion of the nucleic acid and for hybridization of the oligonucleotides attached to the particle with the second portion of the nucleic acid so as to form a test substrate having one or more particle complexes bound thereto; applying a stringency wash to the substrate to substantially remove any non-specifically bound particles and any particle complexes having oligonucleotides that are not complementary to the second portion of the nucleic acid sequence; contacting the test substrate with a staining material to produce a detection substrate having a surface capable of causing surface-enhanced Raman scattering (SERS); and determining for the presence of any particle complexes on said detection substrate as an indication of the existence of a single nucleotide morphism in said nucleic acid in the sample by obtaining and analyzing a SERS spectrum..

In the foregoing methods for detecting nucleic acid as the target, the nucleic acid is first contacted with the substrate so that the first portion of the nucleic acid sequence hybridizes with complementary oligonucleotides bound to the substrate and then the nucleic acid bound to the substrate is contacted with the particles having Raman labels and oligonucleotides bound thereto so that at least some of the oligonucleotides bound to the particles hybridize with the second portion of the sequence of the nucleic acid bound to the substrate.

Alternatively, the nucleic acid is first contacted with the particles having Raman labels and oligonucleotides bound thereto so that at least some of the oligonucleotides bound to the particles hybridize with a second portion of the sequence of the nucleic acid; and then contacting the nucleic acid bound to the particles with the substrate so that the first portion of the sequence of the nucleic acid bound to the particles hybridizes with complementary oligonucleotides bound to the substrate. In another embodiment, the substrate has a plurality of types of oligonucleotides attached thereto in an array to allow for the detection of multiple portions of a single type of nucleic acid, the detection of multiple types of nucleic acids, or both. Alternatively, the nucleic acid, detection probe, and capture oligonucleotides bound to the substrate can be contacted simultaneously.

In another aspect of the invention, at least two or more different Raman active labels are used in the detection probe. The ratio of the two or more types of Raman labels may be the same or different In yet another embodiment of the invention, a detection probe or reagent is provided.

The reagent comprises a specific binding complement for binding to a specific target analyte and at least one type of Raman active label bound theretowherein the Raman active label serves as S an identifier for a specific target analyte; and (ii) the Raman active label comprises at least one Raman active molecule providing a detectable or measurable Raman scattering signal when Silluminated by radiation capable of inducing a Raman scattering.In another embodiment of the invention, the detection probe comprises a particle, a specific binding complement for binding to a specific target analyte, and at least one type of Raman active label wherein the Raman active C label serves as an identifier for a specific target analyte; and (ii) the Raman active label comprises at least one Raman active molecule providing a detectable or measurable Raman C scattering signal when illuminated by radiation capable of inducing a Raman scattering. The Raman label may be bound directly or indirectly to the particles. Alternatively, the Raman label N1 may be attached to the specific target analyte and the conjugate is then attached to the particle.

O In yet another embodiment of the invention, the detection probe comprises a particle, C1 a specific binding complement bound to the particle for binding to a specific target analyte, at least one type of Raman active lable wherein the Raman active label serves as an identifier for a specific target analyte; and (ii) the Raman active label comprises at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering, and oligonucleotides bound to the particle wherein at least some of the Raman labels are bound to at least a portion of the oligonucleotides.

In another aspect of this embodiment of the invention, the detection reagent comprises a particle, oligonucleotides bound to the particle and at least one type of Raman label bound to a portion of the oligonucleotides, wherein at least some of the oligonucleotides bound to the particle have a sequence that is complementary to at least a portion of a target nucleic acid.

In another aspect of the invention, the detection reagent comprises a particle, oligonucleotides bound to the particle, an oligonucleotide connector having first and second portions, an oligonucleotide having at least one type of Raman label bound thereto, wherein at least some of the oligonucleotides bound to the particles have a sequence that is complementary to the first portion of the oligonucleotide connector, the oligonucleotide having the Raman active label bound thereto has a sequence that is complementary to the second portion of the oligonucleotide connector, and at least a portion of the oligonucleotides bound to the particles have a sequence that is complementary to a target nucleic acid.

In yet another aspect of the invention, the reagent comprises a particle, oligonucleotides bound to the particle, an oligonucleotide connector having first and second portions, an oligonucleotide having at least one type of Raman label bound thereto, and an oligonucleotide having a specific binding complement to a target analyte, wherein at least some of the oligonucleotides bound to the particles have a sequence that is complementary to the first portion of the oligonucleotide connector, the oligonucleotide having the Raman active label bound thereto has a sequence that is complementary to the second portion of the oligonucleotide connector, and the oligonucleotide having the specific binding complement bound thereto has a sequence that is complementary to the second portion of the oligonucleotide connector.

In another embodiment of the invention, a kit is provided for the detection of one or more target analytes in a sample. The kit has in one container a detection reagent as described above such as a reagent comprising a particle having a specific binding member and at least one Raman label bound to the particle; a staining reagent; and a substrate having a capture reagent A representative kit comprises: one or more types of conjugates comprising particles, oligonucleotides bound to the particles, a Raman label bound to at least a portion of the oligonucleotides, wherein at least some of the oligonucleotides attached to each type of particle have a sequence that is complementary to a second portion of the sequence of a specific target nucleic acid; and (ii) the Raman active label bound to each type of particles is different and serves as an identifier for a specific target nucleic acid, said Raman active label comprising at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering, an optional substrate having oligonucleotides bound there, the oligonucleotides bound to the substrate have a sequence that is complementary to a first portion of a sequence of the target nucleic acid; and optional stain reagents for creating a substrate surface capable of causing surfaceenhanced Raman scattering (SERS).

In another embodiment of the invention, a kit is provided for the detection of one or more target analytes in a sample, the sequence of the nucleic acid having at least two portions. The kit comprises: particles comprising oligonucleotides bound thereto, a Raman label bound to at least a portion of the oligonucleotides, wherein at least some of the oligonucleotides attached to the particle have a sequence that is complementary to a second portion of the sequence of the target nucleic acid; and (ii) the Raman active label bound to the particles serves as an identifier for the target nucleic acid, said Raman active label comprising at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering; and an optional substrate having oligonucleotides bound there, the oligonucleotides bound to the substrate have a sequence that is complementary to a first portion of a sequence of the target nucleic acid; and In another embodiment of the invention, a kit is provided for the detection of one or more target nucleic acids in a sample, the sequence of the nucleic acid having at least two portions.

The kit comprises: a first container including oligonucleotides having Raman active labels attached thereto, wherein the oligonucleotides the Raman active label comprising at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering; a second container including conjugates comprising particles and oligonucleotides bound to the particles, wherein at least some of the oligonucleotides attached to each type of particle have a sequence that is complementary to at least a portion of the sequence of the oligonucleotides having Raman active labels; and an optional substrate having oligonucleotides bound there, the oligonucleotides bound to the substrate have a sequence that is complementary to a first portion of a sequence of the target nucleic acid; and optional stain reagents for creating a substrate surface capable of causing surfaceenhanced Raman scattering (SERS).

In another embodiment of the invention, a kit is provided for the detection of one or more target nucleic acids in a sample, the sequence of the nucleic acid having at least two portions.

The kit comprises: one or more containers including oligonucleotides having one or more types of Raman active labels attached thereto, wherein the Raman active label comprising at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering; a second container including conjugates comprising particles and oligonucleotides bound to the particles, wherein at least some of the oligonucleotides attached to each type of particle have a sequence that is complementary to at least a portion of the sequence of the oligonucleotides having Raman active labels; and an optional substrate having oligonucleotides bound there, the oligonucleotides bound to the substrate have a sequence that is complementary to a first portion of a sequence of the target nucleic acid and optional staining material reagents.

In another embodiment of the invention, a method for screening one or more molecules to determine whether the molecule is a ligand to one or more specific receptors, the molecules are present in a sample, is provided. The method comprises: providing a substrate having bound thereto one or more specific receptors; providing reagents comprising particles, specific binding substance bound to the particles, a Raman active label bound to a portion of the specific binding substance, and the molecule from said sample bound to a portion of the specific binding substance, wherein said Raman active label comprising at least one Raman active molecule providing a detectable or measurable Raman scattering signal when illuminated by radiation capable of inducing a Raman scattering; contacting the particles, sample and substrate under conditions effective for specific binding interactions between the molecule bound to the particles with the specific receptor bound to the substrate so as to form a test substrate having particles complexed thereto when the molecule is a ligand to a specific receptor, contacting the test substrate with a staining material to produce a detection substrate having a surface capable of causing surface-enhanced Raman scattering (SERS); and determining for the presence of said particle complexes on said detection substrate as a confirmation of a ligand to a specific receptor by obtaining and analyzing a SERS spectrum.

The invention also includes in another aspect a fiber optic analyte detection device in which a particle reagent with specific binding substance and Raman labels is associated with the ends of optical fibers in an optical cable.

These and other embodiments of the invention will be apparent in light of the detailed description below.

Detailed description of the invention: Definitions "Analyte," or "target analyte", as used herein, is the substance to be quantitated or detected in the test sample using the present invention. The analyte can be any substance for which there exists a naturally occurring specific binding member an antibody, polypeptide, DNA, RNA, cell, virus, etc.) or for which a specific binding member can be prepared, and the analyte can bind to one or more specific binding members in an assay. "Analyte" also includes any antigenic substances, haptens, antibodies, and combinations thereof. The analyte can include a protein, a peptide, an amino acid, a carbohydrate, a hormone, a steroid, a vitamin, a drug including those administered for therapeutic purposes as well as those administered for illicit purposes, a bacterium, a virus, and metabolites of or antibodies to any of the above substances.

Other examples of analytes that can be detected or quantitiated according to the invention include polysaccharides, lipids, lipopolysaccharides, glycoproteins, lipoproteins, nucleoproteins, oligonucleotides, and nucleic acids. Specific analytes include antibodies, immunoglobulins, albumin, hemoglobin, coagulation factors, peptide and protein hormones insulin, gonadotropin, somatropin), non-peptide hormones, interleukins, interferons, other cytokines, peptides comprising a tumor-specific epitope an epitope found only on a tumor-specific protein), cells red blood cells), cell-surface molecules CD antigens, integrins, cell receiptors), microoriganims (viruses, bacteria, parasites, molds, and fungi), fragments, portions, components or products of microorganisms, small organic molecules digoxin, heroin, cocaine, morphine, mesaline, lysergic acid, tetrahydrocannabinol, cannabinal, steroids, pentamindine, and biotin), etc. Nucleic acids and oligonucletides that can be detected or quantitiated include genes a gene associated with a particular disease), viral RNA and DNA, bacterial DNA, fungal DNA, mammalian DNA human DNA), cDNA, mRNA, RNA and DNA fragments, oligonucleotides, synthetic oligonucleotides, modified oligonucleotides, signle-stranded -and double-stranded nucleic acids, natural and synthetic nucleic acids, etc.

Essentially any analyte can be detected or quantitaed using antibodies speicifc for the anlyte. In addition, any molecule which binds specifically to the analyte can be used, and many such molecules are known in the art. For instance, nucleic acids can be detected or quantiated using oligonucleotides having a sequence which is complementary to at least a portion of the analyte nucleic acid. Also, lectins can be used to detect or quantiate polysaccharides and glycosylated proteins. As another example, a receptor can be used to detect its ligand and vice versa.

"Analyte-analog", as used herein, refers to a substance which cross reacts with an analyte specific binding member although it may do so to a greater or lesser extent than does the analyte itself. The analyte-analog can include a modified analyte as well as a fragmented or synthetic portion of the analyte molecule so long as the analyte analog has at least one epitopic site in common with the analyte of interest "Analyte epitope," as used herein, denotes that part of the analyte which contacts one member of the specific ligand binding pair during the specific binding event That part of the specific binding pair member which contacts the epitope of the analyte during the specific binding event is termed the 'paratope." "Analyte-mediated ligand binding event," as used herein, means a specific binding event between two members of a specific ligand binding pair, the extent of the binding is influenced by the presence, and the amount present, of the analyte. This influence usually occurs because the analyte contains a structure, or epitope, similar to or identical to the structure or epitode contained by one member of the specific ligand binding pair, the recognition of which by the other member of the specific ligand binding pair results in the specific binding event. As a result, the analyte specifically binds to one member of the specific ligand binding pair, thereby preventing it from binding to the other member of the specific ligand binding pair.

"Ancillary Specific binding member," as used herein, is a specific binding member used in addition to the specific binding members of the captured reagent and the indicator reagent and becomes a part of the final binding complex. One or more ancillary specific binding members can be used in an assay of the invention. For example, an ancillary specific binding member can be used in an assay where the indicator reagent is capable of binding the ancillary specific binding member which in turn is capable of binding the analyte.

"Associated," as used herein, is the state of two or more molecules and/or particulates being held in close proximity to one another.

"Capture reagent," or "capture probe" as used herein, is a specific binding member capable of binding the analyte or indicator reagent, which can be directly or indirectly attached to a substantially solid material. The solid phase capture reagent complex can be used to separate the bound and unbound components of the assay.

"Conjugate," as used herein, is a substance formed by the chemical coupling of one moiety to another. An example of such species include the reaction product of bovine serum albumin with chemically activated theophylline molecules and the reaction product of chemically activated Raman-active labels with a protein molecule, such as an antibody, or with a ligand, such as biotin.

"Enhancer," or "enhancing agent" as used herein, is a stain such as a silver or gold stain that provides for activating Raman labels on particles to produce a SERS effect.

"Indicator reagent," as used herein comprises a detectable label directly or indirectly attached to a specific binding member or metal surface.

"Intervening molecule," as used herein, is any substance to which both a specific binding pair member and a Raman-active label are attached.

'Tarticles," as used herein, is any substance which can be dispersed in a liquid and which will support the phenomenon of a surface-enhanced Raman light scattering (SERS) or surfaceenhanced resonance Raman light scattering (SERRS). Examples of particles include, but are not limited to: Colloids of gold or silver, Pt, Cu, Ag/Au, Pt/Au, Cu/Au, coreshell or alloy particles; particles, hollow particles, or flakes of gold, silver, copper, or other substances displaying conductance band electrons. As the particle surface participates in the SERS and SERRS effect, flakes or particles of substances not displaying conductance band electrons, which have been coated with a substance which does, also become suitable particulates. Particles include nanoparticles such as metallic nanoparticles.

"Radiation," as used herein, is an energy in the form of electromagnetic radiation which, when applied to a test mixture, causes a Raman spectrum to be produced by the Raman-active label therein.

"Ramnan label," as used herein, is any substance which produces a detectable Raman spectrum, which is distinguishable from the Raman spectra of other components present~ when illuminated with a radiation of the proper wavelength. Other terms for a Ramnan-active label include dye and reporter molecule. Such labels are discussed further below.

"SERRS (Surface Enhanced Resonance Raman Scattering)" results when the adsorbate at a SERS active surface is in resonance with the laser excitation wavelength. The resultant enhancement is the product of the resonance and surface enhancement "SERS (Surface-Enhanced Ramnan Scattering)" means the increase in Ramnan scattering exhibited by certain molecules in proximity to certain metal surfaces.

"Specific binding member," as used herein, is a member of a specific binding pair, i.e., two different molecules where one of the molecules, through chemical or physical means, specifically binds to the second molecule. In addition to antigen and antibody-specific binding pairs, other specific binding pairs include biotin and avidin, carbohydrates and lectins, complementary nucleotide sequences (including probe and captured nucleic acid sequences used in DNA hybridization assays to detect a target nucleic acid sequence), complementary peptide sequences, effector and receptor molecules, enzyme cofactors and enzymes, enzyme inhibitors and enzymes, cells, viruses and the like. Furthermore, specific binding pairs can include members that are analogs of the original specific binding member. For example a derivative or fr-agment of the analyte, an analyte-analog, can be used so long as it has at least one epitope in common with the analyte. lInmunoreactive specific binding members include antigens, haptens, antibodies, and complexes thereof including those formed by recombinant

DNA

methods or peptide synthesis.

"Test mixture," as used herein, means a mixture of the test sample and other substances used to apply the present invention for the detection of analyte in the test sample. Examples of these substances include; Specific binding members, ancillary binding members, analyteanalogs, Raman-active labels, buffers, diluents, and particulates with a surface capable of causing a surface-enhanced Raman spectroscopy, and others.

"Test sample," as used herein, means the sample containing the analyte to be detected and assayed using the present invention. The test sample can contain other components besides the analyte, can have the physical attributes of a liquid, or a solid, and can be of any size or volume, including for example, a moving stream of liquid. The test sample can contain any substances other than the analyte as long as the other substances do no interfere with the specific binding of the specific binding member or with the analyte or the analyte-analog. Examples of test samples include, but are not limited to: Serum, plasma, sputum, seminal fluid, urine, other body fluids, and environmental samples such as ground water or waste water, soil extracts, air and pesticide residues.

Reagents The present invention contemplates the use of any suitable particle having Raman labels and specific binding substances attached thereto that are suitable for use in detection assays. In practicing this invention, however, nanoparticles are preferred. The size, shape and chemical composition of the particles will contribute to the properties of the resulting probe including the DNA barcode. These properties include optical properties, optoelectronic properties, electrochemical properties, electronic properties, stability in various solutions, pore and channel size variation, ability to separate bioactive molecules while acting as a filter, etc. The use of mixtures of particles having different sizes, shapes and/or chemical compositions, as well as the use of nanoparticles having uniform sizes, shapes and chemical composition, are contemplated.

Examples of suitable particles include, without limitation, nano- and microsized core particles, aggregate particles, isotropic (such as spherical particles) and anisotropic particles (such as nonspherical rods, tetrahedral, prisms) and core-shell particles such as the ones described in U.S.

Patent application no. 10/034,451, filed December 28, 2002 and International application no.

PCTI/S01/50825, filed December 28, 2002, which are incorporated by reference in their entirety.

Nanoparticles useful in the practice of the invention include metal gold, silver, copper and platinum), semiconductor CdSe, CdS, and CdS or CdSe coated with ZnS) and magnetic ferromagnetite) colloidal materials. Other nanoparticles useful in the practice of the invention include ZnS, ZnO, TiO 2 AgI, AgBr, HgI2, PbS, PbSe, ZnTe, CdTe, In2S3, In 2 Se3, Cd 3 P2, Cd 3 As 2 InAs, and GaAs. The size of the nanoparticles is preferably from about 1.4 nm to about 150 nm (mean diameter), more preferably from about 5 to about 50 nm, most preferably from about 10 to about 30 nm. The nanoparticles may also be rods, prisms, cubes, tetrahedra, or core shell particles.

Methods of making metal, semiconductor and magnetic nanoparticles are well-known in the art. See, Schmid, G. Clusters and Colloids (VCI, Weinheim, 1994); Hayat, M.A Colloidal Gold: Principles, Methods, and Applications (Academic Press, San Diego, 1991); Massart, IEEE Taransactions On Magnetics, 17, 1247 (1981); Ahmadi, T.S. et al., Science, 272, 1924 (1996); Henglein, A. et al., J. Phys. Chem., 99, 14129 (1995); Curtis, et al., Angew. Chem. Int. Ed. EngL, 27, 1530 (1988).

Methods of making ZnS, ZnO, TiO2, AgI, AgBr, HgI2, PbS, PbSe, ZnTe, CdTe, In 2 S3, In 2 Se3, Cd 3

P

2 Cd 3 As 2 InAs, and GaAs nanoparticles are also known in the art. See, e.g., Weller, Angew. Chem. Int. Ed. Engl., 32, 41 (1993); Henglein, Top. Curr. Chem., 143, 113 (1988); Henglein, Chem. Rev., 89, 1861 (1989); Brus, Appl. Phys. 53, 465 (1991); Bahncmann, in Photochemical Conversion and Storage of Solar Energ (eds. Pelizetti and Schiavello 1991), page 251; Wang and Herron, J. Phys. Chem., 95, 525 (1991); Olshavsky et al., J. Am. Chem. Soc., 112, 9438 (1990); Ushida et al., J. Phys. Chem., 95, 5382 (1992).

Suitable nanoparticles are also commercially available from, Ted Pella, Inc. (gold), Amersham Corporation (gold) and Nanoprobes, Inc. (gold).

Presently preferred for use in detecting analytes are gold nanoparticles. Gold colloidal particles have high extinction coefficients for the bands that give rise to their beautiful colors.

These intense colors change with particle size, concentration, interparticle distance, and extent of aggregation and shape (geometry) of the aggregates, making these materials particularly attractive for colorimetric assays. For instance, hybridization of oligonucleotides attached to gold nanoparticles with oligonucleotides and nucleic acids results in an immediate color change visible to the naked eye. Suitable nanoparticles, including core-shell nanoparticles, and methods for preparing such nanoparticles are described for instance in assignee Nanosphere, Inc.

PCT/US01/01190, filed January 12, 2001; PCT/US01/10071, filed March 28, 2001; PCT/US01/46418, filed December 7, 2001; PCT/US01/050825, filed December 12, 2001; and PCT/US02/16382, filed May 22, 2002, the disclosures where are incorporated herein in their entirety.

Attachment of Specific Binding Members The particles, the specific binding member or both may be functionalized in order to attach the specific binding member to the particles to produce detection probes. Such methods are well known in the art. For instance, oligonucleotides functionalized with alkanethiols at their 3'-termini or 5'-termini readily attach to gold nanoparticles. See Whitesides, Proceedings of the Robert A. Welch Foundation 39th Conference On Chemical Research Nanophase Chemistry, Houston, TX, pages 109-121 (1995). See also, Mucic et al. Chem. Commun. 555-557 (1996) (describes a method of attaching 3' thiol DNA to flat gold surfaces; this method can be used to attach oligonucleotides to nanoparticles). The alkanethiol method can also be used to attach oligonucleotides to other metal, semiconductor and magnetic colloids and to the other nanoparticles listed above. Other functional groups for attaching oligonucleotides to solid surfaces include phosphorothioate groups (see, U.S. Patent No. 5,472,881 for the binding of oligonucleotide-phosphorothioates to gold surfaces), substituted alkylsiloxanes (see, e.g.

Burwell, Chemical Technology, 4, 370-377 (1974) and Matteucci and Cauthers, J. Am. Chem.

Soc., 103, 3185-3191 (1981) for binding of oligonucleotides to silica and glass surfaces, and Grabar et al., Anal. Chem., 67, 735-743 for binding of aminoalkylsiloxanes and for similar binding of mercaptoaklylsiloxanes). Oligonucleotides terminated with a 5' thionucleoside or a 3' thionucleoside may also be used for attaching oligonucleotides to solid surfaces. The following references describe other methods which may be employed to attached oligonucleotides to nanoparticles: Nuzzo et al., J. Am. Chem. Soc., 109, 2358 (1987) (disulfides on gold); Allara and Nuzzo, Langmuir, 1, 45 (1985) (carboxylic acids on aluminum); Allara and Tompkins, J. Colloid Interface Sci., 49, 410-421 (1974) (carboxylic acids on copper); Iler, The Chemistry Of Silica, Chapter 6, (Wiley 1979) (carboxylic acids on silica); Timmons and Zisman, J. Phys. Chem., 69, 984-990 (1965) (carboxylic acids on platinum); Soriaga and Hubbard, J. Am. Chem. Soc., 104, 3937 (1982) (aromatic ring compounds on platinum); Hubbard, Acc. Chem. Res., 13, 177 (1980) (sulfolanes, sulfoxides and other functionalized solvents on platinum); Hickman et al., J Am.

Chem. Soc., 111, 7271 (1989) (isonitriles on platinum); Maoz and Sagiv, Langmuir, 3, 1045 (1987) (silanes on silica); Maoz and Sagiv, Langmuir, 3, 1034 (1987) (silanes on silica); Wasserman et al., Langnmuir, 5, 1074 (1989) (silanes on silica); Eltekova and Eltekov, Langmuir, 3, 951 (1987) (aromatic carboxylic acids, aldehydes, alcohols and methoxy groups on titanium dioxide and silica); Lec et al., J. Phys. Chem., 92, 2597 (1988) (rigid phosphates on metals).

U.S. patent application nos. 09/760,500 and 09/820,279 and international application nos. PCT/US01/01190 and PCT/US01/10071 describe oligonucleotides functionalized with a cyclic disulfide which are useful in practicing this invention. The cyclic disulfides preferably have 5 or 6 atoms in their rings, including the two sulfur atoms. Suitable cyclic disulfides are available commercially or may be synthesized by known procedures. The reduced form of the cyclic disulfides can also be used.

Those skilled in the art recognize a large variety of methods by which nucleic acids, antigen, antibodies, proteins, peptides, small molecules, carbohydrates or any specific binding member can be bound directly or indirectly to particles. For example, linkers may be used to attach the specific binding member to the nanoparticle.

Substrates Any substrate can be used which allows observation of the detectable change. Suitable substrates include transparent solid surfaces glass, quartz, plastics and other polymers), opaque solid surface white solid surfaces, such as TLC silica plates, filter paper, glass fiber filters, cellulose nitrate membranes, nylon membranes, PDVF membranes), and conducting solid surfaces indium-tin-oxide The substrate can be any shape or thickness, but generally will be flat and thin. Preferred are transparent substrates such as glass glass slides or glass beads) or plastics wells ofmicrotiter plates).

The ends of optical fiber in a fiber optical cable serve also as a substrate in one embodiment of the invention. As an alternative to DNA detection in a surface microarray format, it is also possible to contact the ends of a fiber optic bundle with a desired reagent. As with the surface array method, the reagent includes a particle with at least one Raman label bound to it; the reagent also includes a specific binding member.

One or more of the optical fibers in the bundle can transmit laser light toward the end of the bundle where the reagent is, at a frequency chosen to stimulate Raman scattering. The fibers that transmit the laser light may be referred to as excitation fibers. When the reagent is thus stimulated, the Raman label is activated, providing a SERS effect Some of the light produced by the SERS effect is backscattered and transmitted into the remaining fibers in the bundle, the collection fibers. This backscattered light can be detected at the other end of the fiber optic bundle. It is possible to process multiple samples with just one laser excitation source and one detector, although multiple lasers and detectors can also be used if required for excitation and detection optimization for different reagents- Attachment of capture probes to a substrate Any suitable method for attaching a capture probe to a substrate may be used. For instance, capture probes comprising oligonucleotides complementary to a nucleic acid target can be attached to the substrates as described in, Chrisey et al., Nucleic Acids Res., 24, 3031- 3039 (1996); Chrisey et al., Nucleic Acids Res., 24, 3040-3047 (1996); Mucic et al., Chem.

Commun., 555 (1996); Zimmermann and Cox, Nucleic Acids Res., 22, 492 (1994); Bottomley et al., J. Vac. Sci. TechnoL A, 10, 591 (1992); and Hegner et al., FEBS Lett., 336, 452 (1993).

When a substrate is employed, a plurality of capture probes may be attached to the substrate in an array for detecting multiple different target analytes. For instance, a substrate may be provided with rows of spots, each spot containing a different type of capture probes designed to bind a reagent analyte complex. A sample containing one or more analytes is applied to each spot, and the rest of the assay is performed in one of the ways described above using appropriate reagents of the invention.

Raman labels The Raman labels can be any one of a number of molecules with distinctive Raman scattering spectra. Unlike the enzymes used in enzyme immunoassays, these label species can be stable, simple, inexpensive molecules which can be chemically modified as required.The following attributes enhance the effectiveness of the label in this application: A strong absorption band in the vicinity of the laser excitation wavelength (extinction coefficient near 104; A functional group which will enable covalent attachment to a specific binding member; Photostability, Sufficient surface and resonance enhancement to allow detection of analyte in the subnanogram range; Minimal interference in the binding interaction between the labeled and unlabeled specific binding members; Minimal exhibition of strong fluorescence emission at the excitation-wavelength used; A relatively simple scattering pattern with a few intense peaks; and/or Labels with scattering patterns which do not interfere with each other so several indicator molecules may be analyzed simultaneously.

The following is a listing of some, but not all potential candidates for these Raman-active label: 4-(4-Aminophenylazo)phenylarsonic acid monosodium salt, arsenazo I, basic fuchsin, Chicago sky blue, direct red 81, disperse orange 3, HABA (2-(4-hydroxyphenylazo)-benzoic acid), erythrosin B, trypan blue, ponceau S, ponceau SS, 1,5-difluoro-2,4-dinitrobenzene, cresyl violet and p-dimethylaminoazobenzene. The chosen labels may be covalently attached to the specific binding members of interest or attached or associated with.

An important aspect of the invention is that multiple Raman labels may be bound to the particle to provide a multicoding Raman labels for indexing different particles. Thus, the invention includes a reagent which has multiple Raman dyes and a specific binding substance, such as DNA, RNA, antibody, antigen, small molecule bound to the particle. For particle-based detection probes, the Raman labels or dyes can be attached directly or indirectly to the particle.

The Raman label can be modified with a functional group, a thiol, amine, or phosphine that can bind to the surface of the particle such as a metallic nanoparticle. If desired, the Raman dye can be further functionalized with a molecule such as oligonucleotides polyadenosine, polythymidine) for enhanced nanoparticle stability or with a specific binding pair member (such as an oligonucleotide having a sequence that is complementary to at least a portion of a nucleci acid target or a receptor for a particular ligand). Alternatively, the Raman label can be conjugated with a molecule or any linker, polyA or polyT oligonucleotide, that bears a functional group for binding to the particle.

The multiple Raman label also need not be bound to the particle but may be complexed to the particle through specific binding reactions. Thus, the invention encompasses multiple SERS reagents bound to a specific binding ligand such as DNA, RNA, antibody, antigen, small molecule, cell or virus. This embodiment may be envisioned as follows: Ramanl -Raman2 Raman3 (specific binding ligand) Excitation Sources In the preferred embodiment, a laser serves as the excitation source. The laser may be of an inexpensive type such as a helium-neon or diode laser. An operating lifetime of such lasers may be in excess of 50,000 hours.

In one embodiment, a diode laser is used to excite at or at the near IR spectrum, minimizing fluorescence interference. The excitation sources used need not necessarily be monochromatic and they also need not necessarily have to be of high intensity. Lamps may also be used.

The SERS effect can be excited by direct illumination of the surface or by evanescent waves from a waveguide beneath the plasmon-active surface.

Raman labeled probes Several different conjugates could be prepared from specific binding members having different specificities, each type with a different Raman active label having a distinctive scattering pattern. Mixing these conjugates in an assay would allow the simultaneous analysis of several different analytes in the same sample. In another aspect of the invention, the conjugate may include two or more different Raman labels.

It is important to note that in contrast with conventional fluorescence-based chip detection, the ratio of Raman intensities can be extracted from a single Raman spectrum using single laser excitation. Moreover, the number of available Raman dyes is much larger than the number of available and discemable fluorescent dyes.

2 0 21 6 A Raman dye can be either fluorescent or non-fluorescent A minor chemical modification of a dye molecule can lead to a new dye with different Raman spectra even though the two dyes exhibit virtually indistinguishable fluorescence spectra 26 Therefore, this Raman fingerprinting method offers potentially greater flexibility, a larger pool of available and non-overlapping probes, and higher multiplexing capabilities than conventional fluorescence-based detection approaches. This approach has been extended to random array, bead based format where high multiplexing capabilities are essential are underway.

SERS Enhancement (Enhancer) Initially, the Raman- labeled probes have little or no detectable SERS activity. Staining material such as silver stains provide strong SERS enhancment. When a substrate is employed, a detectable change can be produced or further enhanced by silver staining. Silver staining can be employed with any type of nanoparticles that catalyze the reduction of silver. Preferred are nanoparticles made of noble metals gold and silver). See Bassell, et al., J. Cell BioL, 126, 863-876 (1994); Braun-Howland et al., Biotechniques, 13, 928-931 (1992). If the nanoparticles being employed for the detection of a nucleic acid do not catalyze the reduction of silver, then silver ions can be complexed to the nucleic acid to catalyze the reduction. See Braun et al., Nature, 391, 775 (1998). Also, silver stains are known which can react with the phosphate groups on nucleic acids.

Silver, gold or copper staining can be used to produce or enhance a detectable change in any assay performed on a substrate, including those described above. In particular, silver staining has been found to provide a huge increase in sensitivity for assays employing a single type of nanoparticle so that the use of layers of nanoparticles can often be eliminated.

Detection of Raman scattering Several methods are available for detecting Raman scattering. These generally can be used with different types of spectrometers. In SERS, the primary measurement is one of light scattering intensity at particular wavelengths. SERS requires measuring wavelength-shifted scattering intensity in the presence of an intense background from the excitation beam. The use of a Raman-active substance having a large Stokes shift simplifies this measurement Several concepts for further simplifying the readout instrument have been proposed.

These include the use of wavelength selective mirrors, filters or holographic optical elements for scattered light collection.

Neither the angle of the incident light beam to the surface nor the position of the detector is critical using SERS. With flat surfaces positioning the surface of the laser beam at 60 degrees to the normal is commonly done and detection at either 90 degrees or 180 degrees to the beam are standard. SERS excitation can be performed in the near infrared range which would suppress intrinsic sample fluorescence. It may also be possible to perform SERS-based ligand binding assays using evanescent waves produced by optical waveguides.

No signal development time is required as readout begins immediately upon illumination and data can be collected for as long as desired without decay of signal unless the excitation light is extremely intense and chemical changes occur. The signal cannot overdevelop as in systems dependent on optical absorbance. Unlike fluorescent readout systemns,. SERS reporter groups will not self-quench so the signal can be enhanced by increasing the number of Raman reporter groups on the probe molecule. Fluorescent molecules near the SERS-active surface will also be surface-quenched.

Instrumentation The present invention is adaptable for use as an automatic analyzer. Since the instrunent would monitor discrete Stokes shifted spectral lines, the need for an elaborate mono'chromator system is not necessary. Recent advances in state-of-the-art optics technology, such as holographic optical elements, allow the design of a suitable spectrometer with cost and complexity below that of the laboratory grade device.

Optical readout energies as a result of SERS are above that which require ultra-sensitive photon counting devices. In fact, some SERRS spectrometers now in use incorporate silicon photodiode detectors. The optical efficiency of a typical monochromator used in a laboratory grade spectrometer is less than 10%. The advances in optical materials and components mentioned above should make possible two to three-fold increaes in optical efficiency for a simple spectrometer dedicated to only a few specific spectral lines. This also addresses one of the previously major concerns, blocking of the Rayleigh scattering line. With blocking capabilities of newer filters on the order of 10-9, substitution of filters for one or more stages of the typical monocbrometer. system should be possible with significant cost savings.

Examples: Example 1: icroarray Fabrication.

Oligonucleotide capture strands were immobilized Onto the SMPB-(MUCCUimiJdYl 4- (malemidophenyl)-butyrate) functionalized glass slide by spotting oligoucloetides (1 mM in a 0.15 M NaCl, pH 6.5 phosphate buffer solution (PBS, 10 UIM phosphate)) with a commercial arrayer (GMS 417 arrayer, Genotic MicroSystems, Inc). Alter spotting the chip with the capture oligonucleotides (-200 Pm spots), the chip was kept in a humidity chamber for 12 hours to effect the coupling reaction between SMPB and the hexylthiolcapped oligonucleotides. Then the chip was washed copiously with Nanopure water.

Passivation of the areas of the chip surrounding the oligonucleotide spots was carried out by immersing the chip in a solution of hexyithiol-capped poly-adenine (Als) (0.1mM) for 4 h and then in a solution of 3-mercapto-propane sulfonic acid, sodium salt (0.2 MW for 30 minutes to cap off the remaining SMPB sites. Finally, the chip was washed with Nanopure water and dried by a inicroarray centrifuge (2000 g).

Example 2: Synthesis and Purification of Cy3~abeled-( Pronvlthiofl-g5Ped Oligonuc-leotides.

This Example describes the synthesis of an oligonucleotide having a Ranman label attached thereto: (3'HS-Cy3-Ajo-AAT CTC AAC GTA CCI?, (SEQ ID NO 1. in Fig 19a) 3' HS--Cy3-Aio-CTC-CCT-AAT-AAC-AAT) (SEQ ID) NO.25 in Figure 1) The Cy3-modified, (propylthiol)-capped oligonucleotides were synthesized on a 1 Pmol scale using standard phosphorainidite chemistry 5with a Thiol-Modifier C3 S-S CPG (controlled-pore glass) solid support on a commercial synthesizer (Expedite). The Cy3-CE phosphoramidite (Indodicarbocyanine 3, 1 '-O-(4monomethoxytrityl)- 1-O-(2-cynoethy)-(NNdiisopropyl)-phosphoramidite (Glen Research) was used to incorporate the Cy3 unit in the oligonucleotides. To aid purification, the final dimethoxytrityl (DNM protecting group was not removed. After synthesis, the CPG-supported oligonucleotides were placed in I m1L of concentrated amnmonium hydroxide for 8 h at 55 C' to cleave the oligonucleotide from the solid support and remove the protecting groups from the bases. In each case, cleavage from the solid support via the succinyl ester linkage produced a mixed disulfide composed of the (mercaptopropyl) oligonucleotide and a mercaptopropanol linker. After evaporation of S ammonia the crude oligonucleotides were purified by preparative reverse-phase KPLC using an B?)HI ODS Hypersil column (300 A, 250 x10 mm, retention ti=e- 32 min) with 0.03 M <triethylammonium acetate (TEAA), pH 7 and a 1%//mmn gradient of 95% CH 3 CN/5% 0.03 M S TEAA at a flow rate of 3 mImin, while monitoring the LTV signal of DNA at 254 nm and 550 nm. The DMT was cleaved by dissolving the purified oligonucleotides in an 80% acetic acid C* solution for 30 mlin, followed by evaporation; the oligonucleotides were redispersed in 500 IiL of S water, and the solutions were extracted with ethyl acetate (3 x300 pL). After evaporation of the solvent, the oligonucleotides were redispersed in 400 pL of a 0.1 M dithiothreotol (DTI), 0.17 M phosphate buffer (pH 8) solution at room temperature for 2 h to cleave the 3' disulfide.

S Aliquots of this solution (<10 ODs) were purified through a desalting NAP-S column (Amersham Pharmacia Biotech AB).

Example 3: Synthesis and Purification of ThIR-, Cy3.5- and caped Oligonucleotides This Example describes the syntheses of three oligonucleotides having Raman labels bound thereto: 3' HS--TMR-Al 0 AAC CGA AAG TCA ATA [SEQ ID NO. 2 in Fig. 19a]; 3'

A

10 -CCT CAT TTA CAA CCT [SEQ ID NO. 3 in Fig. 19a]; and 3'HS-Cy5- Ajo- CTC CCT AAT AAC AAT [SEQ ID NO. 4 in 19b]. Because the dyes are sensitive to standard cleavage reagent (ammonia), ultramild base monomers (from Glen Research) were used here to allow the deprotection reaction under ultamild conditions: phenoxyacetyl (Pac) protected dA, 4isopropyl-phenoxyacetyl (iPr-Pac) protected dG, and acetyl (Ac) protected dC. TAMRA-dT (TMR-dT, 5!-Dimethoxytrityloxy-5[N((tetramethylrhodmifyl)amflnohexyl) 3 acryhmido]- 2 dex~iie3-2caoty)(,-ispoy)-hshrmdt) Cy3 phosphoramidite (Indodicarbocyanine 3.5, 1 '-O-(4-monomethoxytityl)-1-O-(2-cyanoethyl)- (N,N-diisopropyl)-phosphoramidite), and CyS-CE phosphoramidite (Indodicarbocyanine 5,11'-0- (4-monomethoxytrityl)-1 -O-(2-cyanoethy)-(N,N-disopropyl)-phosphoramidite) were used to label the oligonucleotides, respectively. After synthesis of the oligonucleotides, the synthesis columontents weretransferred to a 23Lreaction vial andtreated with I mL of 0.05M potassium carbonate in anhydrous methanol for 4 h at room temperature. Then the supernatant 31 Swas pipetted from the support and neutralized with 1.5 xnL of 2M triethYaflmoflimf acetate.

Further purification was carried out as described above for the synthesis of the Cy3-abeled- 01) ;Z oligonucleotides. I{PLC retention times are 28, 32, 30 mein for TMIR-, Cy3.5- and propyitbiol-capped oligonucleotides, respectively.

Example 4: Synthesis and Purification of Rhodamine 6G-, and Texas Red-labeled- (DroPYltbio)-aWed Oligonucleotides This Example describes the synthesis of two olignucleotides have Raman labels attached thereto: 3'HS-Rd-Aio-TCA ACA TI'G CCT TCT [SEQ ID NO. 5 in Fig. l9b] and 3' HS-TR-

SA

10 -TCT TCT ATA AAC CT]? ATT [SEQ ID NO. 6 in Fig 19a]. See Figure 24. Both of these oligonucleotides were prepared via two-step syntheses. In the first step, amino-modified oligonucleotides 2 )-Aj 0 -TCA ACA TTG CCA TCT and 3-S-S(NH 2

)-A

0

-TCT

TCT ATA AAC CT]? ATT)were synthesized via literature procedures The amino-mdiier C6 dT5-iehxtiy--N(rfura~tlaioe))3arlmd]2-ex~iie cyanoethyl)-(N,N-diisopropyl)]-phosphoraidite) was placed in position 5 in the synthesizer (Expedite), and amino-modified oligonucleotides were obtained by conventional automated syntheses. The cleavage, deprotection, and purification of the oligonucleotides were carried out by the procedures described for the synthesis of the Cy3-modifled oligonucleotide (above), retention time 26 mein. In the second step, succinimide ester modified Rhod 6G rhodareine 6G, succinimidyl ester) and Texas Red (Texas Red-X-succinlimidyl ester) were coupled to the amino-modified oligonucleotides, respectively. In a typical experiment an amino-modified, alkylthiol-capped-oligonucleotide (0.15 pinol) was dissolved in a sodium borate buffer (0.IM, 0.5 ml), and a DMSO solution (150 p.1) containing 2.5 mg of the succinimide ester modified Rhod 6G (or Texas Red) was added to the oligonucleotide buffer solution, Figure 24. The solution was stirred at room temperature for 12 hr. Then, the Rhod 6G- (or Texas red-) labeled oligonucleotide was purified by ethanol precipitation (3 times) and Rfuther by HPLC in the conditions as described above.

Example 5: DNA detection assa In a typical experiment for DNA detection, a three-component sandwich assay is used in microarray format (Figure Gold nanoparticles (13 :L 2 r m in diameter) modified with Cy3labeled, alkylthiol-capped oligonucleotide strands were used as probes to monitor the presence of specific target DNA strands. These nanoparticle conjugates were prepared in accordance with the aging process described in U.S. Patent No. 6,506,564 (Nanosphere, Inc., assignee), issued January 14, 2003, which is incorporated by reference in its entirety. On average, there are 110 oligonucleotide strands on each 13-rm gold nanoparticle. The Cy3 group was chosen as a Raman label due to its large Raman cross section.

23 A chip spotted with the appropriate 15 mer capture strands was coated with a 0.6 M NaCI PBS buffer solution (10 mM of phosphate, pH 7) containing a 30 mer target sequence (100 pM) in a humidity chamber at room temperature.

After 4 h, the chip was washed four times with 0.6 M NaC1 PBS buffer solution to remove nonspecifically bound target. Then, the chip was treated with a 0.6 M NaCi PBS solution of nanoparticle probes (2 )M for 1.5 hour to effect hybridization with the overhanging region ofihe target sequence (Figure The chip was then washed with 0.6 M NaN0 3 PBS buffer solution to remove chloride ions and nonspecifically bound nanoparticle probes. The chip was immediately treated with a silver enhancement solution (Ted Pella, Inc) for 8 minutes, subsequently rinsed with Nanopure water, and dried with a microarray centrifuge (2000 The chip, which exhibits grey spots visible to the naked eye, could be imaged with a flatbed scanner (Expression 1600, Epson) via literature procedures, Figure 2A and B.8 The spots also were imaged by Raman spectroscopy in a 0.3 M NaC1 PBS buffer solution (Solution Rmnan 633 spectrometer from Detection Limit Inc., 30 mW He-Ne laser), Figure 2C. The chip was scanned with a fiber-optic probe with a 0.65 N.A. adapter (25 pm laser spot), and each spot shows a consistent and strong Raman response at 1192 cm 1 (Figure 2D).

Prior to silver enhancing, the nanoparticle probes were invisible to the naked eye, and no Raman scattering signal was detectable (Figure 2A). This is due to a lack of electromagnetic field enhancement for the undeveloped nanoparticles (13 rn in diameter) in this state.

24 -26 Others have shown that closely spaced gold nanoparticles in such sizes can give surfaceenhanced Raman scattering enhancement, 27 3 0 but for DNA detection at technologically relevant target concentrations riM), ianoparticle spacings are too large to yield such effects. After silver enhancing, the Ag particles can grow around the Cy3-labeled nanoparticle probes leading to large Raman scattering enhancements. Typically, the obtained spectra include both sharp 33 to 30 cm-) Raman lines and a concomitant broad underlying continuum as noted by Brus et. al.

in their studies of Rhodamine 6G molecules on Ag particles.

3 m 31 Importantly, the Raman scattering signals arise almost exclusively from the Cy3 dye molecules immobilized on the particles; no signals were observed from other species such as the oligonucleotides, solvent molecules, and the succinimidyl 4-(maleimidophenyl)-butyrate (SMPB) on the glass surface.

Moreover, the Raman scattering frequency for each Raman line remains constant from experiment to experiment, deviating by less than 2 cm Since consistent SERS signals from the Cy3-labeled nanoparticle probes were obtained, the Raman spectrum of Cy3 can be used as a spectroscopic fingerprint to monitor the presence of a specific target oligonucleotide strand.

Example 6: Detection ofDNA at Low Target Concentration (examle: 20 fM) In a typical experiment, a chip spotted with the appropriate capture strands (Figure 3A) was coated with a 0.75M NaCI PBS buffer solution (10 mM of phosphate, pH 7) containing a target sequence (20 fl) in a humidity chamber at room temperature. After 8 h, the chip was washed with 0.75M NaC1 PBS buffer solution to remove nonspecifically bound target.

Then, the chip was treated with a 0.75 M NaC1 PBS solution of nanoparticle probes (500 pM) for 3 h to effect hybridization with the overhanging region of the target sequence (Figure 3A). The chip was washed with 0.75 M NaNO 3 PBS buffer solution to remove chloride ions and nonspecifically bound nanoparticle probes. The chip was immediately treated with silver enhancement solution (from Ted Pella, Inc) for 15 min, subsequently rinsed with Nanopure water, and dried with a microarray centrifuge (2000 The spots can be imaged in the dry state with a flatbed scanner (Figure 3B) or by Raman spectroscopy in the wet state (0.3 M NaC1, pH7, PBS buffer solution), Figure 3C and D. The current unoptimized detection limit with this technique is 10 fM.

Example 7: Detection of multiple oligonucleotide targets This Example describes detection of multiple oligonucleotides using a plurality of Raman labeled probes. One can utilize the approach described in Example 5 and nanoparticle probes functionalized with dyes other than Cy3 to create a large number of probes with distinct and measurable SERS signals. This allows multiplexed detection of a large number of 34 oligonucleotide targets simultaneously. To demonstrate this point six commercially available dyes were selected with distinct Raman spectra that can be incorporated into oligonucleotides through standard automated DNA-syntheses. Six types of Raman labeled and oligonucleotidemodified gold nanoparticle probes were prepared with sequences that were respectively complementary to statistically unique 30-36 mer sequences for. Hepatitis A virus Vall7 polyprotein gene (HIVA), Hepatitis B virus surface antigen gene (HYB), H1Y, Ebola Virus OMV, Variola virus (smallpox, WY), and Bacillus anthracis (BA) protective antigen gene (Figure 4)32 With these probes, the multiplexing capabilities of the novel scanning Raman technique for the six target analytes can be demonstrated.

Bight separate tests were carried out to evaluate the selectivity of the system and our ability to determine the number and types of strands in solutions containing mixtures of the different targets (Figure 4 and The concentrations of the target strands were kept constant for all of these experiments (100 pM each), and the hybridization conditions were as described above. In the first test (Figure 5, row all spots show the same intense grey color associated with silver deposition. However, they can be differentiated simply by using the Raman scanig method, and once the spectroscopic fingerprint of the Ag-containing spot has been determined the correct Raman label and, therefore, target sequence can be identified. To simplify the analysis, a color (rectangular box) to each Raman labeled probe (Figure 4 and Figure 5B) was assigned. In the first test (Figure 5A), all six targets were present, and all show strong grey scale values when measured via the flatbed scanner and the expected Raman fingerprints. In the next seven tests, one or more of the targets to evaluate the suitability of this method for multiplexing were systematically removed. Note that with the single color grey scale method one cannot determine if any cross hybridization has occurred. However, with this "multiple color" scanning Raman method, one can carefully study the SERS spectra of each spot to determine which labels make up each spot. For the experiments described in Figure 5, where the sequences are very dissimnilar, it was found that other than the expected spectroscopic probe signature for each target, there are virtually no other detectable Raman lines, which means that there is no crosshybridization between different targets and probes.

It should be mentioned that the obtained SERS signal only comes from areas of the substrate where the Raman dye-labeled gold particles have initiated Ag formation. Therefore, this "multiple color" scanning Raman detection method does not record background signal due to silver deposition where Au particles do not exist. This is not the case for the previous greyscale scanometric approach, especially at ultra-low target concentrations (<50 flM).

Example 8: Discrimination and Ratioing of Single Nucleotide Polymorphisms (SNPs) in Oligo-ribonucleic Acid (RNA) Targets.

This Example describes the use of oligonucleotides having Raman labels in detection systems to differentiate single nucleotide polymorphisms (SNPs), and in the case of gene expression studies, one would like access to RNA detection with single spot signal ratioing capabilities. It is well known that nanoparticle probes heavily functionalized with oligonucleotides exhibit extraordinarily sharp thermally-induced denaturation transitions that lead to substantially higher selectivity than conventional molecular fluorophore probes in DNA detection.

5 However, nothing is known about the behavior of these probes in the context of RNA detection. To further test the selectivity of this Raman based system and its ability to identify SNP targets, two RNA targets were chosen that can bind to the same capture strand DNA but have a single-base mutation in the probe binding regions (target l:Tj, normal; target 2:T 2 single-base difference, Figure Therefore, two DNA-fmnctionalized probes (probe 1: P

I

probe 2: P2), which differ in sequence and Raman label, are required to differentiate these two RNA target strands (Figure Seven separate tests were performed to demonstrate not only how the two targets (TI and T 2 can be differentiated but also how mixtures of the two targets can be analyzed in semi-quantitative fashion.

In a typical experiment, the appropriate capture strands (Figure 6) were spotted in quadruplicate on SMPB functionalized glass slides. These slides were coated with 0.3M NaCI PBS buffer solutions (10 mM of phosphate, pH 7) containing pure RNA target 1 or target 2, or mixtures of 1 and 2 (1 nM total oligonucleotide concentration) in a humidity chamber at room temperature. After 2 h, the chip was washed four times with 0.3M NaC1 PBS buffer solution to remove nonspecifically bound target. Then, the chip was* treated with a 0.3 M NaCI PBS solution of nanoparticle probes (2 nM, probel: probe2=l:l) for 1.5 h to effect hybridization with the overhanging region of the target sequences (Figure The chip was washed with 0.3 M NaNO3 PBS buffer solution to remove chloride ions and nonspecifically bound nanoparticle probes. If the chips were developed by silver enhancing, the Rmnan measurements on the grey spots at different target ratios yield similar spectra (Figure which are nearly identical to the spectrumn for the sample containing probe 1 and probe 2 in equal amounts. This result indicates that there are equal amounts of probe 1 and probe 2 on the chip. This is because the stabilities of the perfectly matched and single-mismatched oligonucleotide duplexes are close in magnitude, and therefore, nanoparticle probes (1 and 2) bound to the spots on the chips in nearly equally amounts at all of the target ratios. Under these conditions the two targets cannot be differentiated.

In each of these tests, a slide was treated with a 0.3 M NaCi PBS buffer solution containing T, and T 2 in different ratios (total concentration =1 nM) in a humidity chamber.

After 2 h, the chip was washed with a 0.3 M NaCi PBS buffer to remove nonspecifically bound target. Then, the chip was treated with nanoparticle probes (PI and P 2 at 1:1 ratio, 2nM total concentration) for 1.5 bi to effect hybridization with the overhanging region of the target sequences (Figure The chip was washed with 0.3 M NaNO 3 PBS buffer solution to remove chloride ions and nonspecifically bound nanoparticle probes. Note that there are four possible hybridization modes, namely, T 1 :Pj, T 2

:P

2

T

1

:P

2 and T 2

:P

1 (Figure If the chip was developed by silver enhancing without prior stringency wash, the Ramnan measurements on the grey spots which correspond to different solution target ratios yield nearly identical spectra in all seven experiments; these spectra also are almost identical to those obtained for a sample containing a 1: 1 ratio of probe I and probe 2 (see Supporting Information). These data show that probe 1 and probe 2 are bound to the spots on the chip in equal amounts, regardless of the target composition on the spot.

Therefore, in order to identify the target composition on the spots, a salt or temperaturebased stringency wash must be applied. Accordingly, a salt stringency wash (8 mM NaCI PBS buffer) was employed to selectively denature the imperfect duplexes (T 1

:P

2 and/or T 2 :Pl, Figure 6C and 6D) but not the duplexes formed from the perfectly complementary oligonucleotides

(T

1 :Pl and/or T 2

:P

2 Figure 6A and 6B).

9 After stringency wash and subsequent silver staining, the Raman measurements on the grey spots can be used to readily identify the target composition on the spots by the obtained spectra. In tests where only pure RNA target 1 or 2 are present, only signals for probe 1 or 2, respectively are observed (compare Figure 9B and In the case 37 of mixtures, signals for both probes (Ii: 1650 cm- 1 from probe land 12: 1588 cm 1 from probe 2) are detected, and the intensity ratios are proportional to the ratios of the two targets in each experiment (inset of Figure 9B).

Example 9: Screening of Protein: Small molecule interaction This Rmnan detection format also can be used in protein microarray applications for screening protein-small molecule and protein-protein interactions. For the detection of proteinsmall molecule interactions, three unrelated small molecules were selected for which the specific protein receptors are commercially available: biotin and its mouse monoclonal antibody, DIG (steroid digoxigenin) and its mouse.monclonal antibody, DNP (dinitrophenyl) and its mouse monoclonal antibody. The three small molecules were labeled with Raman dye-funmctionalized gold particles: the gold particles (13 nm in diameter) were modified with a small-molecule capped, Raman dye and alkylthiol-functionalized poly-adenine(A2o) (Figure 11A). In a typical detection experiment, the proteins from all three pairs were immobilized in triplet onto aldehydefunctionalized glass slides by spotting the protein solution (200 pLg/ml, 5% glycerol) with a commercial arrayer (Figure 11 A).

333 4 After 4-hour incubation in a humidity chamber, the protein chip was washed with PBS buffer (0.173 M NaCI, 0.027 M KC1, 4.3 mM Na 2

HPO

4 1.4 mM KH 2 PO4, pH=7.4) containing 0.5% bovine serum albumin (BSA), and immersed into such solution for 4 hour to passivate the unreacted aldehydes on the protein chip. After being washed with a PBS solution (0.173 M NaCl, 0.027 M KCI, 4.3 mM Na 2 HPO4, 1.4 mM KH1 2

PO

4 pH=7.4), the protein chip was treated with Raman labeled small molecule probes (for 2 hours at 4 After washed with a buffer solution (0.2 M NaNO3, 5 mM phosphate, pH=7.4), the gold particle functionallized protein chip was treated with the silver enhancement solution for 8 minutes and washed with Nanopure water. Before Raman measurements, the silver stained chip was immersed in a 2 x PBS solution for 10 minutes.

In the first test, the protein chip was exposed to all a solution containing all three Ramanlabeled small molecule probes. After silver enhancement, the triplet dot array is clearly visible, even to the naked eye (Figure 11B-1). When measuring the Raman spectra of the dots, the correct probe spectra was obtained with no evidence of cross reactivity less than Cy3 for biotin, Cy3.5 for DIG, and Cy5 for DNP). Next the same type chip was studied but in the presence of the DIG and DNP probes, and gain obtained the expected results, Figure 11B-2 and C2). All other possible two probe combinations was studied and again the expected results were obtained, demonstrating the high selectivity of the system (Figure 11B-3, C-3 and 11B4, C-4).

In the two probe experiments, one probe for the array is absent, serving as a control for screening the other interaction pairs.

Example 10: Screening Protein-Protein interactions For screening protein-protein interactions, three pairs of proteins were chosen for study: mouse immunoglobulin G (IgG) and its antibody, ubiquitin and its antibody, human protein C and its antibody. Mouse IgG, ubiqutin, and human protein C were spotted in quadruplicate on aldehyde slides, respectively. Gold nanoparticles were first functionalized with antibodies and then with Raman-dye labeled oligonucleotides. The labeling procedure is shown in Figure 12: an antibody (10 ig, pH= 9 2 was put into a solution of gold particles (13 nm, 10 nM, lmL, pH=9.

2 for 20 minutes, and then the Raman dye capped-alkylthiol-functionalized poly-adenine (Alo, 0.2 OD at 260 nm) was added to the solution. After 12 hours, 10% BSA solution (0.3 mL) was added to the solution to further passivate the surface of the gold particles. The solution was allowed to stand for 10 minutes. The Raman-dye capped gold particle-antibody conjugates were purified by centrifugation (14,000 rpm), which precipittaes the particles. The supernatant containing excess oligonucleotide, BSA, and antibodies can be decanted from the particles. The particle probes are then be redispersed in PBS buffer. The probes (2nM for gold nanoparticles, about 2 pg/ml for the antibodies) were then used to develop the protein chips. The protocol for screening the protein-protein interactions is similar to that for protein-small molecule interactions (described above).

The chip in Figure 13 A-4 was probed with all the three Raman labeled antibodies simultaneously. After silver enhancement, all three two-by-two dots array are clearly visible after silver develioping. Raman analysis shows no detectable cross reactivity and all of the correct dyes are in the correct spots (Figure 13).

Just like fluorophore-based methods, this new scanometric detection format provides a general approach for genomic and porteomic detection but with a higher sensitivity and a higher multiple labeling capability. The number of available Raman dyes is much larger than the number of available and discemable fluorescent dyes.

20 21 A Raman dye can be a fluorescent dye O) and also a non-fluorescent dye. A small modification of a dye can lead to a new dye with S different Raman spectra and even the dyes which show undistinguishable fluorescent spectra can be distinguished by Raman spectroscopy.

1 6 In the conventional multicolor fluorescent dyes labeling format, the data readout requires multi-lasers and multiple scans.' By contrast, only a C\ single laser and individual scan are required in this Raman scanometric detection format, CS suggesting a potential for a high throughput reading process. Although quantum-dot-labeled M fluorescence detection requires only single laser, multicolors are usually generated from different size and shape quantum-dot nanoparticles.

6 7 Different sized and shaped nanoparticles associated O biological labels will have different thermodynamic and kinetic properties, which are problematic for parallel microarray biological detection. In the Raman scanometric detection format, in contrast, only one-sized gold nanoparticle (13 nm, here) carriers are required, and labeling information from different Raman dyes. Therefore, most of the labels described here have similar thermodynamic and kinetic target binding properties, which are essential for faster, more-accurate, high-throughput microarray based mapping and screening ofbiomolecules.

1 Example 11: Multiple Raman-dve labeled nanoparticle probes All the Raman labels described above are single-dye systems: one carrier and Raman dye.

One can load two or multiple Raman dyes onto a nano-sized nanoparticle carrier. Massively encoded Raman labels can be generated by tailoring the ratio between the components (Figure 14 and 15). In a two-dye system, two alkylthiol capped-oligonucleotide strands with same base sequences but different the Raman labels (Cy3 and TMR) were used to modify 13-nm gold nanoparticles simultaneously, and therefore a composite Raman label was generated. This twodye labeled nanopaticle probe has similar thermodynamic and kinetic properties as the single-dye labeled nanoparticle probe same hybridization kinetics and melting temperatures with identical strands). In a typical DNA detection experiment (target concentration is 100 pM, Figure a Raman spectrum from a silver-stained spot clearly shows characteristic Raman lines from both of Cy3 and TMR (Figure 16, left). By varying the ratio of Cy3 and TMR, different composite Raman spectra are obtained (Figure 16, right). These Raman spectra are distinguishable from each other by differences in relative intensities for the main bands in the O region interrogated. The multiple reference windows increase the accuracy for identifying Sdifferent Raman labels, making this two dye Raman labeling methodology practically usable.

S Beyond two-dye systems, two examples of three-dye labels, which have different amount ratio between Cy3, TMR and Cy3.5, are shown in Figure 17.

C N One can use one-dye, two-dye, three-dye and even larger combination-labeled systems.

A significant question is: how many labels can be achievable in this Raman labeling system? In a two-dye system, assuming five intensity levels 1, 2, 3, there are 13 labels that can be generated. Five million labels and three billion labels can be generated with 10-dye and 14-dye S systems, respectively (Figure

C

1 Example 12: Microbead-based Biological Detection Large numbers of parallel labeling techniques are of particular importance in microbeadbased biological detection strategies. Microbead technology is emerging as an important biological analysis format for gene expression monitoring, SNP genotyping, proteomic screening, and drug discovering.

1 13 Compared with the microarray technique, microbead detection shows more flexibility in hybridization-based procedures, faster analyte diffusion kinetics, and they are easier and cheaper to produce. The microbead detection without the positional encoding in the microarrys, however, must rely on some sort ofbarcoding strategy for the particle probes. A major problem in the current fluorescent-dye-based encoding approach is that the number of distinguishable labels are limited due to the broad emission spectra and energy transfer between organic dyes.

1 1 Raman labeling, in contrast, can overcome these difficulties.

For a typical DNA target detection system, a three-component sandwich assay format can be used. In our experiments, glass microbeads (210-250 mm in diameter) were functionalized with oligonucleotide capture strands (Figure 18). Gold nanoparticles (13 nm in diameter) modified with pure or mixed Raman dye-labeled and alkylthiol-capped oligonucleotides probe strands were synthesized. Then the Raman dye and gold particle associated probes (2 nM for gold particles) co-hybridized with the target strands onto the surface of the capture strand oligonucleotide functionalized glass microbeads in a 4 x PBS buffer solution (0.6 M of NaC1, mM of phosphate buffer( pH=7) for 2 hours and washed with a second buffer solution (0.6 M NaNO3, 10 mM phosphate) to remove chloride ions, and non-specifically bound nanoparticle 0D) labels, and immediately treated with a silver enhancement solution (from BBIntemational) for 8 minutes. Before Raman measurements, the microbeads were immersed in a 2 x PBS buffer for min to further enhance Raman scattering signal.

To demonstrate the multiplexing capabilities of the novel scanning Raman technique in microbead detection format, an eight-target analyte detection experiment was chosen. The a sequences of target, capture and probe oligonucleotide strands are shown in Figure 19 a and b.

The corresponding Raman spectra (marked by colored circle and rectangular boxes) are listed in S Figure 20. In a typical experiment, eight capture strands were loaded onto microbeads, 0 respectively. Mixing all the microbeads together, a flexible "random microarray" was built Then the eight targets (100 pM) and Raman-labeled nanoparticle probes (2 nM) are introduced to the random microarray solution under hybridization conditions as described above. After washing and silver staining, the microbeads are show up as dark-grey spheres and exhibit the expected Raman signatures (Figure 21). To achieve an easy readout process, these microbeads were aligned mechanically (Figure 22 top) and read in serial fashion via scanning Raman spectroscopy. (Figure 22, bottom). Moreover, the Raman fingerprints of the micorbeads can also be read out by fiber optics (Figure 23).

Beside this new Raman labeling technique, two recent strategies show the practical potential for massively parallel labeling abilities: quantum-dot-tagged microbeads and submicrometer metallic barcodes.

11 3 5 However, both of these strategies achieve multiple labeling based on micron-size structures. In contrast, Raman labeling here is a nano-size labeling methodology, and has much more flexibility than those micro-size ones. In particular, the footprints of the probes are smaller and the specificity and sensitivity of systems based on the probes can be dramatically improved over the systems based upon larger structures. This new nanoparticle-based methodology is important for a variety of reasons. First, in contrast with conventional fluorescence-based chip detection, the ratio of Raman intensities can be extracted from a single Raman spectrum using single laser excitation. Second, the number of available Raman dyes is much larger than the number of available and discernable fluorescent dyes.20 2 1 6 Indeed, a Raman dye can be either fluorescent or non-fluorescent, but a minor chemical modification of a dye molecule can lead to a new dye with a different Raman spectrum even though the two dyes exhibit virtually indistinguishable fluorescence spectra.

2 Therefore, this fingerprinting method offers potentially greater flexibility, a larger pool of available and nonoverlapping probes, and higher multiplexing capabilities than conventional fluorescence-based detection approaches. Finally, the method incorporates all of the previous advantages of goldnanoparticle based detection, including several orders of magnitude higher sensitivity and many orders of magnitude higher selectivity than the analogous molecular fluorescence based approach.

Example 13: Raman labeling for Blotting Detection All of the Raman detection experiments described above were carried out on the surface of glass chips, or glass beads. However, the selection of substrates is also very flexible. Polymer nitrocellulose, PVDF) substrates also work well for Raman detection experiments, exhibiting no substantial background from the polymer substrates that are typically used for Southern, Northern, and Western blotting experiments (Figure 25). Therefore, the Raman. labeling technique described in this Example can also be applied to Southern, Northern, and Western blotting experiments.

Prior to Western blotting, pre-stained molecular-weight labels (aprotinin, lysozyme, soybean trypsin inhibitor and carbonic anhydrase) were loaded in one well of a pre-cast tris-HCI polyacrylamide gel, and ubiquitin was loaded in another well of the same polyacrylamide gel (the samples were dissolved in a loading buffer solution (130 fg/ 250 pL): 60 mM Tris-HC, 2% SDS (dodecyl sulfate, sodium salt), 5 mM f-mercaptoethanol, 0.005% bromophenol blue, glycerol). By applying an electric field (200 V, constant voltage) (PowerPac BasicTM Power Supply, from Bio-Rad Laboratories, Hercules, CA 94547)], these protein samples were separated in the polyacrylamide gel (SDS buffer solution: 25 mM Tris, 192 mM Glycine, and 0.1% (w/v) SDS, pH Then, the protein samples were transferred onto a nitrocellulose membrane by applying an electric field for 2 h (400 mA, constant current; transfer buffer: 25 mM Tris, 192 mM Glycine, and 20% methanol, pH The prestained protein labels were clearly shown in blue on the membrane, but ubiquitin was not. Then, the nitrocellulose membrane was washed with a buffer solution (20 mM, Tris buffer, pH 7.6, 150 mM NaCI) for three times, and then placed into a milk solution (20 mM, Tris buffer, pH 7.6, 150 mM NaC, 5% dry milk) to passivate 43 the area without protein (Figure 26) for 12 h. After passivation, the membrane was probed with anti-ubiquitin probes (Figure 27A, 2nM for gold nanoparticles, about 2 g/ml for the antibodies, see Example 10). The gold particle fimunctionalized membrane was treated with the silver enhancement solution (Ted Pella, Inc., Redding,) for 8 minutes, and the area with gold nanoparticle probes was shown out in dark gray due to silver staining (Figure 27B). Before Raman measurements, the silver stained membrane was immersed in a 2x PBS solution for 10 minutes. A typical Raman spectrum from the dark gray area on the membrane exhibits the Raman lines exclusively from Cy3.5, but not from the nitrocellulose membrane, the protein molecules, and the other chemicals in the experiments (Figure 27C). These results demonstrate that the Raman labeling described herein can be applied to blotting experiments.

References 1. G. Gibson, S. V. Muse, A Primer of Genome Science (Sinauer Associates, Inc. Sunderland, MA, 2002).

2. M. Schena, D. Shalon, R. W. Davis, P.O. Brown, Science 1995,270,467.

3. M. Chee, R. Yang, E. Hubbell, A. Bemno, X C. Huang, D. Stern, JI Winkler, D. J. Lockhart, M. S. Morris, S. P. A. Fodor, Science 1996, 274, 610.

4. M. Snyder, et al., Science 2001, 293, 2101.

Elghanian, I. I Storhoff, R C. Mucie, R. L. Letsinger, C. A. Mirkin, Science 1997, 277, 1078.

6. M. Bruchez Jr., M. Moronne, P. Gin, S. Weiss, A. P. Alivisatos, Science 1998, 281, 2013.

7. W. C. W. Chan, S. Mhe, Science 1998, 281, 2016.

8. T. A. Taton, C. A. Mirkin, R. L. Letsinger, Science 2000, 289, 1757.

9. S. J. Park, T. A. Taton, C. A. Mirkin, Science, 2002, 295, 1503.

L. He, M. D. Musick S. Nicewarner, F. Salinas, S.3J. Benkovic, M. JI Natan, C. D.

Keating, J Am. Chem Soc. 2000, 122,9071.

11. M. Han, X Gao, I. Z. Su, S. Nhe, Nature Biotechnology 2001, 19, 63 1.

12. 1. Willner, F. Patoisky, I. Wasserman, Angew. Chem. Int. Ed. 2001, 40, 2261.

13. H. Mattoussi, J. M. Mauro, E. R. Goldman, G. P. Anderson, V. C. Sundar, F. V. Mikulec, M.

G. Bawendi, J Am. Chem. Soc. 2000,122, 12142.

14. S. Pathak S. K Choi, N. Aruheirn, M. E. Thompson, J Am. Chzem. Soc. 2001, 123, 4103.

S. Schultz, D. Smith, 3. 1. Mock, D. A- Schultz, Proc. N~at. Acad. Sci. U.S.A. 2000, 97, 996.

16. C. M. Niemeyer, Angew. CThem. Int. Ed. 2001, 40,4128.

17. See website: www.nanosphere-inc.com.

18. C. A. Mirkin, AACC Meeting, San Diego, CA, 2001.

19. D. Walt Science 2000, 287,451.

D. Graham, B. I. Mallinder, W. E. Smith, Angew. Chem. Int. Ed 2000, 39, 1061.

.21. D. Graham, W. E. Smith, A. M. T. Linacre, C. 11 Munro, N. D. Watson, P. C. White, Anal.

Chem 1997, 69,4703.

22. L. M. Demers, C. A. Mirin, R- C. Mucic, R- A. Reynolds II R. L. Letsinger, Elghanian, G. Viswanadhain, Anal. Chem 2000, 72, 5535.

23. R- L. McCreery, Raman spectroscopy for chemical analysis (John Wiley Sons, New York, 2000).

24. G. C. Schatz, R. P. Van Duyne, in Handbook of Vibrational Spectroscopy M. Chahmers and P. R. Griffiths, Ed., Wiley, Now York, 2002, page:759-77 4 A- Campion, P. Kainbhampati. Chem. Soc. Rev. 1998,27,241.

26. K. Kneipp, H. Kneipp, L ltzkn, R- R. Dasari, M. S. Feld, Chem. Rev. 1999, 99, 2957.

27. S. R. Emory, S. Nie, J Phys. Chem B 1998, 102,493.

Hommer, M. A. Jackson, P. C. Smith, D. G. Walter, M. J. Natan, Science 1995,267,1629.

29. M. D. Musick, C. D. Keating, M. H. Keefe, M. J. Natan, Chem. Mater. 1997,9,1499.

A. M. M ichaeis, M. Nirina], L. E. Brus, J. Am Chem. Soc. 1999,121, 9932.

31. A. M. Michaels, IJi ang, L. Bras, J Phys. Chem B 2000, 104, 11965.

32. From the web site of National Center for Biotechnology Iformation (NCBL): http://www2.ncbimlnfI.gov/Gebakrmfdex.htm~l.

3 3. G. MacBeath, S. L. Schreiber, Science 2001, 289, 1760.

34. B. B. Haab, M. I. Dunhamn, and P.O0. Brown, Genome Biology 2001, 2(2): research0004. 1-0004.13.

S. R Nicewarner-Pefi', R. G. Freeman, B. D. Reiss, L. He, D. 3. Pefifia, L D. Walton, R.

Cromer, C. D. Keating, M. J. Natan. Science 2001, 294, 137.

Claims (4)

1. A reagent comprising a particle having bound there to at least one Raman label and a specific binding member wherein the Raman label can be activated to provide a SERS effect labels. ci|

2. The reagent of claim 1 wherein the particle has two or more different Raman ci

3. The reagent of claim 1 wherein the specific binding member is a DNA, RNA, polypeptide, antibody, antigen, carbohydrate, protein, peptide, amino acid, carbohydrate, hormone, steroid, vitamin, drug, virus, polysaccharides, lipids, lipopolysaccharides, glycoproteins, lipoproteins, nucleoproteins, oligonucleotides, antibodies, immunoglobulins, albumin, hemoglobin, coagulation factors, peptide and protein hormones, non-peptide hormones, interleukins, interferons, cytokines, peptides comprising a tumor-specific epitope, cells, cell-surface molecules, microorganisms, fragments, portions, components or products of microorganisms, small organic molecules, nucleic acids and oligonucleotides, metabolites of or antibodies to any of the above substances.

4. The reagent of claim 3 wherein nucleic acids and oligonucleotides comprise genes, viral RNA and DNA, bacterial DNA, fungal DNA, mammalian DNA, cDNA, mRNA, RNA and DNA fragments, oligonucleotides, synthetic oligonucleotides, modified oligonucleotides, single-stranded and double-stranded nucleic acids, natural and synthetic nucleic acids. The reagent of claim 1 wherein the particle is a gold, Ag, Cu, Pt, Ag/Au, Pt/Au, Cu/Au coreshell and alloy particles. The reagent of claim 1 wherein the particle is a nanoparticle. The reagent of claim I wherein the particle is a metallic nanoparticle. -47