AU2007209781B2 - Dietary methods for canine performance enhancement - Google Patents

Description

AUSTRALIA Patents Act COMPLETE SPECIFICATION (ORIGINAL) Class Int. Class Application Number: Lodged: Complete Specification Lodged: Accepted: Published: Priority Related Art: Name of Applicant: The IAMS Company Actual Inventor(s): Eric Karl Altom, Gary Mitchell Davenport, Russell Lee Kelly, Allan John Lepine Address for Service and Correspondence: PHILLIPS ORMONDE & FITZPATRICK Patent and Trade Mark Attorneys 367 Collins Street Melbourne 3000 AUSTRALIA Invention Title: DIETARY METHODS FOR CANINE PERFORMANCE ENHANCEMENT Our Ref: 809403 POF Code: 103357/455980 The following statement is a full description of this invention, including the best method of performing it known to applicant(s): S-o q DIETARY METHODS FOR CANINE PERFORMANCE ENHANCEMENT Background of the Invention This present application is a divisional application from Australian patent application number 2003221931 the entire disclosure of which is incorporated herein 5 by reference. The present invention generally relates to diet based methods for improving the physical performance and cognitive performance of animals, such as endurance, olfactory perception, improved responsiveness to Pavlovian conditioning, alertness, and target detection. More specifically, the present invention provides methods for 10 improved hunt or search performance of sport and task animals, such as hunting dogs and search dogs. Many canine food formulations on the market are so-called maintenance diets which are designed and formulated to provide adequate nutrition for the average companion dog on a day- to-day basis. There are also special-purpose diets designed to 15 meet special needs or specific nutritional requirements, such as those encountered in pregnancy, nursing, high stress periods, or growth. A number of scientifically designed special-purpose diet formulas are commercially available which can provide desired outcomes in an animal, for example, growth, maintenance, or high energy. The amount of food an animal requires for maintenance or growth depends upon, for example, age,, 20 activity, and environment. An important objective of animal owners, trainers, or care givers is to provide their animal with a diet which maintains overall health, well being, appearance, and prevents disease of the animal; all at a reasonable cost. Another objective is to provide a diet which promotes optimum performance in specific tasks, for example, high 25 energy expenditure activities associated with show, sport, or work animals. Performance diets are known in the art and typically contain, for example, about 25 35% by weight protein and 18-22% by weight fat, and can be used mainly for working or show dogs, pregnant or nursing bitches, or animals under stress. However, performance diets are not recommended for all pets, particularly those with a sedentary 30 lifestyle unless food allowances are controlled. Hunting with dogs for sport or in competitive events is a popular pastime in the United States and elsewhere around the world. The breed or type of dog that is used varies with the type of game that is hunted, as well as the terrain and distances that are covered. Regardless of these la differences, most hunting involves several hours of endurance activity interrupted by short periods of intense running or sprinting. If a dog is hunted often during a season, major nutritional concerns are feeding to promote optimal performance and providing enough calories to maintain body weight and body condition. As a result, nutritional programs for these canine athletes must be designed to provide ample energy and other nutrients to support muscle contraction during athletic events while allowing the dog to benefit from training over the course of the season. Both the immediate needs of the muscle as well as the longer-term concerns such as aerobic capacity, proneness to injury and blood volume must be met with the proper nutritional strategy. (Reinhart, G.A., Nutrition for Sporting Dogs- In: Canine Sports Medicine and Surgery, Bloomberg, M.S., Dee, J.F., Taylor, R.A., eds., Philadelphia, 1998, W.B. Saunders, pp 349.) The nutrient needs of canine athletes have received increased attention in recent years, resulting in improved understanding of the changes that are associated with athletic conditioning in this species. The majority of published reports have studied racing greyhounds, endurance sled dogs, or dogs exercising on treadmills in a laboratory setting. ( Kronfeld, D.S., Diet and the Performance of Racing Sled Dogs, J. Am. Vet. Med. Assoc., 162:470B473, 1973; Hammel, E.P., et al., Metabolic Responses To Exhaustive Exercise in Racing Sled Dogs Fed Diets Containing Medium, Low and Zero Carbohydrate, Am. J. Clin. Nutr., 1976; 30:409-418; Rose, R.J., et al., Responses to Sprint Exercise in the Greyhound: Effects on Hematology, Serum Biochemistry and Muscle Metabolites, Res. Vet. Sci. 1989, 47:212-218; Toll, P.W., et al., The Effect of Dietary Fat and Carbohydrate on Sprint Performance in Racing Greyhound Dogs, In: Proc 8'h Internal. Racing Greyhound Symp. 1992, Gainesville, FLA, pp. 1-3.) Much less is known about the nutritional needs of other types of working dogs. These include, but are not limited to, dogs that are used for hunting, herding, obedience competitions, agility events, and tracking tests, as well as dogs trained to assist the disabled. For each of these categories, the intensity of training and amount of physical work that the dog is required to do can vary considerably. Nevertheless, working dogs typically have increased energy needs compared with the maintenance requirements of normal, adult dogs. An issue is the magnitude of this increase, as well as the best way to supply both energy and other essential nutrients to these working dogs to support maximum performance and well-being. 2 Dietary methods for improving mammalian energetic performance are known, reference for example the following patent. In U.S. Pat. No. 6,159,942, issued December 12, 2000, to St. Cyr, et al., there is disclosed a method whereby precu.rsors of adenosine triphosphate are administered orally to increase intracellular ATP concentration as dietary supplements or for treatment of reduced energy availability resulting from strenuous physical activity, illness or trauma. Pentose sugars are administered individually, mixed into dry food or in solution. The preferred pentose is D-ribose, singly or combined with creatine, pyruvate, L-camitine and/or vasodilating agents. Additionally, magnesium, electrolytes, fatty acids and hexose sugars can be used. The compositions and methods of this invention are especially beneficial to mammals having reduced energy availability or high energy demand. In U.S. Pat. No. 6,156,355, issued December 5, 2000, to Shields, Jr., et al., there is disclosed breed-specific dog food formulations that comprise chicken meat as the major ingredient, rice as the predominant (or sole) grain source, fruit and/or vegetable fiber as the primary or sole fiber source, unique fat and antioxidant blend, vitamins, herbs and spices, carotenoids, and no corn or artificial colors, preservatives, flavors or sugars. Other patents related to the formulation of animal diets include U.S. Patent Nos. 5,616,569 (fermentable fiber), 5,932,258 (glucose metabolism) and 6,133,323 (beta-carotene). The effects of diet on physical performance and olfaction have been noted, see Altom, E., "Effect of Dietary Fat and Physical Conditioning on the Metabolic and Physiological Responses of the Canine Athlete," Ph.D. Dissertation, Auburn University, Auburn, Alabama, 168 pages, March 19, 1999. Certain unsaturated fatty acids, such as the essential fatty acids, are known to play a role in, for example, neural development (see Nutritional Reviews, Vol. 59, No. 8, S34, Aug 2001), retinal composition (see Lipids, Vol. 33, No. 12,1187, 1998), and inflammation response (see Nutrition, Vol. 16, No. 11/12, 1116, 2000). There exists a need for simple, nutritional based methods which enhance muscle and cognitive performance in high activity mammals, that is, mammals that are involved in regular or 3 highly energetic physical activity, for example, a trained dog participating in hunt or search activities. A need also exists for a method to increase the energy level of mammals to provide an increased feeling of well-being, alertness, and lower body temperature during periods of high physical activity and caloric expenditure. 5 A reference herein to a patent document or other matter which is given as prior art is not to be taken as an admission that that document or matter was, in Australia, known or that the information it contains was part of the common general knowledge as at the priority date of any of the claims. Throughout the description and claims of the specification, the word "comprise" and 10 variations of the word, such as "comprising" and "comprises", is not intended to exclude other additives, components, integers or steps. Summary of the Invention Applicant has discovered diet based methods for improving the hunt performance or 15 search performance of canines, for example, improved detection of prey animals or search objects, improved endurance, improved metabolism of diet, reduced feeding costs, and improved body temperature regulation during high activity hunt or search periods. The present invention provides methods for: increasing the hunt or search performance of a hunting or searching mammal which 20 comprises orally administering to the mammal an effective amount of a diet comprising eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), or both, in a total amount of the diet greater than about 0.20 weight percent; increasing the heat endurance of a hunting or searching mammal which comprises orally administering to the mammal an effective amount of a diet comprising EPA, DNlA, or 25 both, in a total amount of the diet greater than about 0.20 weight percent; maintaining the body temperature of a hunting or searching mammal which comprises orally administering to the mammal an effective amount of a diet comprising EPA, DHA, or both, in a total amount of the diet greater than about 0.20 weight percent; and increasing the hunt or search performance of an English Pointer dog which comprises 30 orally administering to the dog for an effective time and in an effective amount a diet comprising EPA, DHA, or both, in a total amount of the diet greater than about 0.20 weight percent. 4 809403 209202 Spcci The present invention also provides a method for increasing the hunt performance of a hunting dog which comprises orally administering to the dog an effective amount of a diet comprising unsaturated fatty acid EPA, unsaturated fatty acid DHA, or both, in a total amount of the diet greater than about 0.2 weight percent; and wherein the diet comprises a total fat 5 content from about 20 to about 28 weight percent and greater than about 70 weight percent of the total fat content of the diet is unsaturated fat and wherein the diet further comprises a saturated fat. The present invention also provides a method for increasing the heat endurance of a hunting dog which comprises orally administering to the dog an effective amount of a diet 10 comprising unsaturated fatty acid EPA, unsaturated fatty acid DHA, or both, in a total amount of the diet greater than about 0.20 weight percent; and wherein the diet comprises a total fat content from about 20 to about 28 weight percent and greater than about 70 weight percent of the=total fat content of the diet is unsaturated fat and wherein the diet further comprises a saturated fat. 15 The present invention also provides a method for maintaining the body temperature of a hunting dog which comprises orally administering to the dog an effective amount of a diet comprising unsaturated fatty acid EPA, unsaturated fatty acid DHA, or both, in a total amount of the diet of greater than about 0.20 weight percent; and wherein the diet comprises a total fat content from about 20 to about 28 weight percent and greater than about 70 weight percent of 20 the total fat content of the diet is unsaturated fat and wherein the diet further comprises a saturated fat. The present invention also provides a method for increasing the hunt performance of a dog which comprises orally administering to the dog for an effective time and in an effective amount a diet comprising unsaturated fatty acid EPA, unsaturated fatty acid DHA, or both, in 25 a total amount of the diet greater than about 0.20 weight percent; and wherein the diet comprises a total fat content from about 20 to about 28 weight percent and greater than about 70 weight percent of the total fat content of the diet is unsaturated fat and wherein the diet further comprises a saturated fat. These and other embodiments are illustrated and exemplified herein. 4a g09403 2(092112 Spmi Detailed Description The following definitions are used, unless otherwise described. A "target find" or "point" in the context of bird-dog hunting is the number of times the bird-dog pointed to and indicated the presence of one or more birds in a specific direction or location during a hunt session. A "target find" or "point" in the context of a search animal is, for example, the number of times the search animal pointed to and indicated the presence of one or more target items or search objects in a specific direction or location during a search session. "Hunt performance" is the number of target finds per hunt. "Overall hunt performance" is a particular animal's total number of "target finds" or "points" per total hours hunted during a hunting season. "Target," "target item," or "target object" can mean, for example, a game animal, prey animal, or search object. "Search performance" or "detection performance" is the number of target finds per total available targets in a search assignment or detection detail. Thus, for example, a search dog finding 8 of 10 known avalanche victims would have a search performance or detection performance of four-fifths or 80 percent. "Search performance", "detection performance", and "hunt performance" are to be construed broadly. In the context of the present invention these terms can be synonymous and interchangeable. "Game animal" or "prey animal" are both broadly construed and include any animal, living or otherwise, which is a desired object of a hunter or hunt animal, and can include, for example, wild or domestic animals, small game, such as squirrel, rabbit, and the like, or larger game, such as fox, boar, deer, bear, buffalo, and like animals. Other common prey animals include, but are not limited to, ground-dwelling or game birds, such as, grouse, turkey, pheasant, quail, dove, partridge, and water fowl, such as ducks and geese, and other common fowl. "Search object" is also construed broadly and can include any living or non-living item, natural or unnatural, which is desired by the searcher or search animal and which search object is to be, for example, discovered, detected, tracked, retrieved, flushed, corralled, and like activities. Examples of search objects include but are not limited to contraband such as narcotics, 5 explosives, herd animals, avalanche or earthquake victims, missing persons, suspects, arrestees, prisoners, prison escapees, trespassers, illegal aliens, terrorists, and the like. "Mammal" can include, for example, wild or domestic animals, such as dogs, cats, ferrets, and like animals. The terms "include", "for example", "such as", and the like are used illustratively and are not intended to limit the present invention. The indefinite articles "a" and "an" mean "at least one" or "one or more" when used in this application, including the claims, unless specifically indicated otherwise. The present invention is exemplified by the following comparative study. English Pointers engaged in bird hunting activities while on a Eukanuba* Adult Premium Performance diet were more successful hunters, for example, recording a statistically significantly greater number of finds, and which Pointers required less food to maintain body weight, body condition, and stool consistency compared with Pointers fed either Purina* Pro Plan Performance or Diamond* diet. Feeding Eukanuba* Adult Premium Performance diet as described and illustrated herein is more economical compared to the Purina*& Pro Plan or Diamondo diets based on daily feeding cost or the cost per find. The oral administration of the diet can be accomplished, for example, by mouth feeding the mammal a weight maintaining amount of the diet. The oral administration can be accomplished by mouth feeding the diet, for example, from 1 to about 10 servings per day, preferably 1 to about 2 servings per day, and more preferably 1 serving per day. The oral administration of the diet over a period of, for example, from about I to about 24 months or longer can produce the desired performance enhancement results and as illustrated herein. The dietary based methods for improved performance of the present invention are particularly applicable where the hunting or searching mammal is a canine, although it will be understood by one skilled in the art that the performance enhancement methods may be applicable to other hunting and working mammals. A particularly preferred canine is an English Pointer breed which is a bird-dog that has been bred, trained, or both, for game bird hunting. The increased hunt performance of the present invention can provide, for example, from about 30 to about 35 percent more target finds, that is for example, points or indications to the presence and location 6 of prey animals per hunt session compared to a above mentioned Purina Pro Plan* chicken and rice diet. The increased hunt performance in embodiments can be about 33 percent more target finds per hunt session over an entire hunting season. In a specific embodiment of the present invention the "overall hunt performance" of English Pointer bird-dogs that were fed the Eukanuba* Adult Premium Performance diet increased by greater than about 30 percent compared to similar dogs fed the Diamond* diet. Not only did the Eukanuba* Adult Premium Performance Formula diet increase the "hunt performance" (the number of target finds per hunt) but also improved the "overall hunt performance" of the hunting animal. Thus the "overall hunt performance" (the number of target finds per hunt or total hours hunted summed over an entire hunting season for a particular animal), in this instance quail birds during a hunting season dramatically improved compared to Diamond formula diet in a carefully controlled blind study and as illustrated herein. Specifically, the method of the present invention increased the overall hunt performance of the hunt animal by about 55 percent compared to the Diamond* formula diet. In embodiments of the present invention the target can be, for example, an animal or search object. In other embodiments, the target can be, for example, an upland game bird, a swimming game bird, or a shorebird, such as a quail or a duck. In cmbodiments of the present invention the post-hunt rectal temperature of the canines administered the Eukanuba* Adult Premium Performance Formula diet was from about 0.5 to about 1.2 degrees Fahrenheit lower compared to canines administered the Purina* Pro Plan diet, and preferably the canines administered the Eukanuba* Adult Premium Performance Formula diet have a post-hunt rectal temperature of from about 1.0 to about 1.1 degrees Fahrenheit lower compared to canines administered the Purina* Pro Plan diet. Thus, the body temperatures of the hunting mammals fed the Eukanubao diet are maintained to within about 3.0 *P of their pre-hunt body temperatures. The oral administration of the diets of the present invention in hunting or searching canines resulted in superior body condition, superior skin condition, and superior coat condition, during the administration period. The oral administration in hunting canines: maintained consistent body weight during the administration period; resulted in excellent and preferred stool properties; maintained excellent overall health of the canines; maintained normal 7 blood chemistry profiles; and maintained normal food attitudes, for example, maintained normal appetites in hunting dogs as compared to increased appetite, or more commonly, loss of appetite in high activity animals. The methods of the present invention provide more efficient and economic food utilization. For example, the Eukanuba* diet used about 2/3 of a cup less food each day (P < 0.05, where P is the probability level) per dog to maintain body weight and body condition during hunting season compared to oral administration of the Purinao Pro Plan diet. The administration Eukanubae diet was more economical (on a cost per gram fed basis) compared to the Purina* Pro Plan diet, for example, by about $0.18 less per day per dog which represents about an $18.00 savings per dog over an entire hunting season. Additionally, on a per "find" annualized basis there was a reduced cost of approximately a $0.29, that is, a "find" costs about 29 cents less per dog per year for dogs fed the Eukanuba* dict formula versus the Purina* Pro Plan diet formula (P <0.05) arising from or directly attributable to reduced food intake, reduced cost of feeding, and improved find performance. In embodiments the present invention provides a dietary composition administered to improve the hunting efficiency of a hunting animal which composition comprises an effective amount of Eukanuba* Adult Premium Performance Formula diet. In embodiments the present invention provides a dietary composition administered to improve the energy utilization efficiency in an active mammal which composition comprises an effective amount of Eukanuba Adult Premium Performance Formula diet. In embodiments the present invention provides a dietary composition administered to avoid or prevent heat exhaustion, such as overheating or heat stroke, in an active mammal which composition comprises an effective amount of Eukanuba* Adult Premium Performance Formula diet. Introduction An investigation was conducted to study the impact, if any, of diet and nutrition on canine hunt performance. Specifically, the effect of commercial diets on the quai)-hunting ability of English Pointers was evaluated at a controlled-access hunting facility. A first year 8 study compared a Eukanuba* formula against aDiamond* formula. A second year study compared a Eukanuba* formula against a Purina* formula. Methods The working ability of English Pointers was measured in this study using a practical response criterion (hunting performance). In terms of the number of points or birds found during a hunting session, the dogs fed Eukanuba* performed better than those fed either Diamond" or Pro Plan* diets. Although such assessments are not easily standardized in a field setting, the two handlers remained blinded to the dietary treatments during both hunting seasons. This control allowed comparisons to be made principally on the dog's hunting success during each season, when the only variation in management was the diet being fed to each group. In the first year of this study, 23 adult Pointers were fed Eukanuba* Adult Premium Performance Formula or Diamond* Premium Adult Dog Food, while in year two, 22 Pointers were fed Eukanuba* Adult Premium Performance Formula or Purina* Pro Plan Chicken & RicO Dog Performance Formula. Dogs were initially offered amounts of food calculated to maintain body weight and body condition based on the NRC recommendation for estimating daily metabolizable energy (ME) requirements (ME = 200 BW""), wherc body weight (BW) is expressed in kilograms and 200 is an activity constant for very active dogs. The handlers were instructed to adjust the food allotment of individual dogs as needed to maintain body weight and body condition during the hunting season. The decision to adjust the food allotment was at the sole discretion of the handler. Food consumption by each dog was measured by the handlers on a regular basis during the study. Body weights and subjective stool scores were also obtained on a regular basis during the season. Subjective stool scoring was conducted using a 5-point scale: 1 = liquid; 2 = soft, no shape; 3 = soft with shape; 4 = firm (ideal); 5 extremely dry. Body condition and skin and coat health were subjectively evaluated in September, November and March to coincide with the initiation of the training and hunting seasons, and the termination of the hunting season, respectively. The 5-point body condition scoring system consisted of: 1 = thin; 2 = underweight; 3 = ideal; 4 = overweight; 5 = obese. Skin and coat evaluations included subjective assessments of skin dander and epilation, and coat shedding, shine, uniformity, density and softness. 9 Individuals blinded to the specific dietary assignments conducted the subjective evaluations for body condition and skin and coat health. All statistical analyses were conducted using the GLM procedures of SAS. For statistical analysis of body weight, body condition and hunting performance data, the hunting season was divided into six 2-week hunting periods. Total times and duration of hunting, number of finds, and finds per hour were calculated on an individual dog basis for each 2-week period because all dogs were not hunted equally during each period. Despite unequal usage during the hunting season, each dog was allowed to hunt at least one time during each 2-week period. These performance data were subsequently analyzed using a statistical model that included the main effects for diet and hunting period and their interaction. Differences among treatment means were assessed by least-squares mean separation using the PDIFF* option of SAS when the respective F-test for the Type HI sums of squares was significant (P<0.10). For all variables, arithmetic means and their respective standard deviations are reported for each treatment group. Principle ingredients, nutrient content and caloric distribution of each dog food are reported in Tables 1 and 2. Daily care and management of the dogs was provided by two professional handlers employed by the hunt facility. The handlers were blinded to the specific diet each dog received. 10 Table 1. Major dietary ingredients of commercial diets fed to English Pointers during the quail hunting season. Eukanubao Diamond* Pro Plan*3 Chicken Chicken by-product meal Chicken Chicken by-product meal Ground corn Corn gluten meal Corn meal Wheat flour Brewers rice Ground grain sorghum Chicken fat Beef tallow Fish meal Brewers rice Ground com Chicken fat Beet pulp Poultty by-product meal Ground whole grain barley Fish meal Cor bran Dried beet pulp Egg product Animal digest Natural chicken flavor Flaxseed Egg product Dried eg product Poultry digest Minerals & vitamins Brewers dried yeast Brewers dried yeast Flax meal Minerals & vitamins Minerals & vitamins 1. The Iams Company, Dayion, OH 45414 2. Diamond Pet Foods, Meta, MO 65058 3. The Ralston Purina Company, S. Louis, MO 63164 11 Table 2. Nutrient content in weight percent or caloric distribution of commercial diets fed to English Pointers during the quail hunting season.' Nutrient Eukanuba" Diamond* Pro Plano Protein %) 31.2 26.1 31.9 Fat (%) 21.4 17.2 21.3 Crude fiber (%) 2.1 3.7 2.0 Moisture (%) 6.7 8.3 7.7 Ash(%) 6.6 6.7 5.6 Carbohydrate (%) 31.9 38.0 31.6 Calcium (%) 1.19 1.50 1.30 Phosphorus (%) 0.97 1.07 .88 Ca:P 1.23 1.40 1.48 Gross energy (kcal/kg) 5,120 4,660 5,139 Metabolizable energy 4,220 3,899 4,224 (ME, kcal/kg)" Protein % of ME 28.1 25.4 28.7 calories)__ Fat (% of ME calories)b 43.1 37.6 42.9 Carbohydrate (% of ME 28.8 37.0 28.4 calories)b Total unsaturated fatty 14.6 11.9 13.3 acids (%) Total omega-3 fatty acids 0.55 0.50 0.15 (%)____ _ omega-6 : omega-3 8:1 8:1 18:1 fatty acid ratio Total EPA + DH4A 0.2440 0.0274 0.0166 (mcan %) EPA: DHA 1.052:1.137 0.66:1.2 0.37:0.44 (mean relative ratio) EPA+DPA+DHA 0.24 0.02 0.02 (total weight percent )C Fat Source(s) fish meal (5), chicken fat(4), beef tallow (4) (label ranking) chicken fat(6), fish meal (5), flaxmeal(12) flaxseed(9) a) Nutrient content was determined by laboratory analyses and are expressed on an as-fed basis. b) Metabolizable energy content and caloric distribution were calculated usng the protein, carbohydrate and fat content and the modified Atwater factors (3.8 kcaIkg; 3.8 kcal/kg; and 8.5 kcal/kg, respectively). 12 c) EPA = eicosapentaenoic acid(20:5n-3); DHA = docosahexaenoic acid (22:6n 3); DPA=docosapentaenoic acid (22:5n-3). - co 2 H EPA

CO

2 H DRA - - co 2 H DPA The above mentioned comparative formulation(s) are all commercially available or may be prepared by procedures known to those in the nutritional arts. Distinguishing aspects between the above Eukanuba* and the Purina* Pro Plan diets follow. The Eukanuba* fat content is chicken-based whereas the Purina* fat content is beef-tallow based. The Eukanuba" protein content is animal-based. The Purina* protein is a combination of animal- and vegetable-based protein. The sources of carbohydrates and omega-3 fatty acids, levels of omega-3 fatty acids, EPA, DPA and DHA, and the ratio omega fatty acids in the Eukanuba* diet are also different from the Purina* diet. Distinguishing aspects between the above Eukanuba* and the Diamond* diets are the protein, fat, and carbohydrate amounts, the carbohydrate source, the levels of EPA, DPA and DHA, and the overall energy content of the diets and as reflected in above tables. The source of dietary fat, the ratio of two fatty acids EPA to DHA, and the total weight percentage of two fatty acids EPA plus DHA in the Eukanuba* diet are different from both the above Diamond* and Purina* diets and as indicated in above table. An analysis of at least seven other commercially available performance diets indicated that all had an EPA to DHA ratio (mean 1.41:1.22), and a total weight percentage of EPA plus DHA(mean 0.0374%) which were both considerably lower than the Eukanuba* diet. 13 The above mentioned methods of the present invention can be accomplished with diets formulated with, for example, a total fat content of from about 20 to about 28 weight percent, for example, from about 20 to about 25, and from about 20 to about 23 weight percent total fat; where greater than about 70 weight percent of the total fat content is unsaturated fat, for example, containing EPA, DRA, or a mixture of EPA and DHA, in a total amount of the diet of greater than about 0.20 weight percent; preferably where greater than about 75 weight percent of the total fat content is unsaturated fat containing EPA, DHA, or a mixture of EPA and DHA in from about 0.21 to about 0.30 weight percent; and more preferably where greater than about 85 weight percent of the total fat content is unsaturated fat containing EPA, DHA, or a mixture of EPA and DHA in from about 0.22 to about 0.28 weight percent. In embodiments the diet can further comprise, for example, a total protein content of about 30 to about 35 weight percent, and a total carbohydrate content of about 30 to about 35 weight percent. In embodiments the unsaturated fat in the diet is preferably poultry fat such as chicken, fish fat, or mixtures thereof, and more preferably a combination of chicken fat, fish meal, and flax meal. The diet is preferably free of beef fat, beef protein, or both. The unsaturated fat content of the high fat-high unsaturated fat, performance diets of the present invention preferably includes a mixture of omega-6 fatty acid and omega-3 fatty acid, for example, in a weight ratio of about 5:1 to about 10:1, and more preferably a ratio of about 8:1, for exampic, as found in the above Eukanuba* diet. A preferred source of omega-3 fatty acid is from, for example, fishmeal, fish oil, and flaxmeal, and more preferably fishmeal or fish oil. A preferred source of the omega-6 fatty acids is from, for example, chicken fat. A preferred type of omega-3 fatty acid is EPA, DPA, or DHA; individually or in combinations. All dogs were subjected to the normal training and hunting program of the private hunt facility located in southwest Georgia, including training, conditioning, and hunting segments. In southwest Georgia, the quail-hunting season extends from mid-November through February and is usually preceded by a 2-month period of individual training and physical conditioning. The respective diets were fed exclusively during the training and hunting seasons. The dogs were fed once daily and they did not receive any supplements or treats. The selection of dogs for hunting and the amount of time they were allowed to hunt were based on the discretion of the handlers. 14 The handlers recorded the dates and total time hunted for each dog, number of finds, number of flushes, general attitude of the dog, and reasons for cessation of hunting. Overall hunting performance was calculated for each dog by determining the total number of finds per hunting session and total hours hunted. Licensed veterinarians, who were also blinded to the dietary treatments, collected blood samples and conducted physical examinations at the initiation and termination of the hunting season, Serum and plasma samples were shipped to a commercial diagnostic laboratory ( Antec Diagnostic, Inc. Farmingdale, NY 11735) for standard veterinary analysis. Because performance in a warm and humid environment is an important consideration for hunting dogs in the southem portion of the United States, a temperature-humidity index (THI) was computed for each day using weather data obtained from a local television station. This index was calculated using the day's high temperature and relative humidity to provide an indication of potential heat stress. (Davenport, G.M., et al., Growth and endocrine responses of cattle to implantation of estradiol-17B during continuous or discontinuous grazing of high-and low-endophyte-infected tall fescue, J. Anim. Sci., 1993, 71:757-764.) Mild, high, and severe levels of heat stress were represented by the values 23.5 to 26, 26.0 to 29.0, and >29.0, respectively. Results The results of a two-year study showed that the nutritional management of hunting dogs can have a significant impact on their hunting performance and working ability. (Davenport, G.M., et al., Effect of Diet on Hunting Performance of English Pointers. Veterinary Therapeutics, 2001, 2:10-23.) The collective results also show that nutritional management of sporting dogs can significantly impact a dog's performance and working ability. Thus, the nutritional management of canine athletes should provide a balanced diet which: is rich in nutrients required by the dog; meets energy requirements when consumed in acceptable amounts; contains optimum protein and fat levels; has a fatty acid profile that minimizes inflammation, for example, of the gut, the skin, and the like; allows for repletion replacementn) of muscle glycogen; contains the amount and type of fiber that promotes a healthy gut; is 15 palatable and readily accepted during periods of stress, such as training, hunting or competition; is convenient to prepare and feed; and is.stable at normal temperatures to avoid rancidity. The results of the year 1 study, comparing Eukanuba with the Diamond* formula, showed that all dogs remained healthy and consumed typical amounts of food throughout the hunting season. Chemical profile and complete blood count (CBC) results were within the normal ranges for adult healthy dogs and showed no major diet-induced changes in the health status of the dogs. Dogs fed EukanubaG maintained or gained weight and body condition throughout the hunting season, while dogs fed the Diamond* diet lost body weight and condition (P<.05). As a result, body weight and body condition scores at the end of the hunting season were higher (P<.05) for dogs fed Eukanuba* compared with the Diamond* diet. No significant differences were observed in fecal stool scores despite a tendency for stools to be slightly softer for dogs fed Diamond*. Dogs fed the Eukanuba* formula demonstrated superior hunting performance (P<. 05) compared with dogs fed the Diamond* formula, based upon total finds per hunt and on the number of birds located per hour of hunting. For the season, dogs fed Eukanuba* had 55% more finds than dogs fed Diamond*, which was equivalent to one more find per hour of hunting. The improved hunting performance of dogs fed Eukanuba* was not due to increased hunting frequency or longer hunting duration, as they were similar for both diet groups throughout the season. During the first year (year 1 study), there were 9 days during the hunting season in which the heat index was rated as high or severe based on the temperature-humidity index. On each of these days, the dogs fed Eukanuba* maintained their superior performance over Diamond* based on the number of finds per hour. In the second year (year 2 study), all dogs remained healthy based on physical examinations, chemical profiles, and CBC results. As in the year 1 study, there were no diet induced changes in the health status of the dogs during the hunting season. Similarly, there were no differences in body weight, body condition, or stool scores during the season for dogs fed the Eukanuba* formula versus the Purina* Pro Plan* formula. Despite these similarities, dogs fed Purina* Pro Plan required 11% more food than dogs fed Eukanuba* to maintain body weight and condition. This increased feeding amount was equivalent to about 2/3 cup more food per 16 day for each dog (6.4 vs. 5.7 cups/day). There were no differences in the frequency or duration of hunting indicating that dogs on both diets had equal opportunities to hunt during this second season. The performance results showed that dogs fed Eukanuba* had 33% more finds during the season than dogs fed Purina* Pro Plan. Over the entire season dogs fed the Eukanuba* formula averaged 3.0 finds per hunt compared with 2.2 finds per hunt for dogs fed the Purina* Pro Plan formula. Weather conditions during the second year were milder than the previous year (year 1 study). As a result, dogs were not subjected to any significant heat-stressing conditions during the hunting season. Discussion Several dietary factors may have influenced the hunting ability and body condition of the dogs used in the studies. Pointers tend to be a highly active breed and individual dogs do not typically carry additional body fat. Furthermore, these dogs typically lose a considerable amount of body condition as the hunting season progresses. Weight loss, even when moderate, is always comprised of both fat and lean body tissue. (Reinhart, G. A., Nutrition for Sporting Dogs. In: Bloomberg MS, Dee JF, Taylor RA, editors: Canine Sports Medicine and Surgery. Philadelphia, 1998, WB Saunders, pp 349; Burgess, N.S., Effect of a very-low-calorie diet on body composition and resting metabolic rate in obese men and women, J. Amer. Diet. Assoc. 1991; 91:430-434; Butterwick, F., et al. Changes in the body composition of cats during weight reduction by controlled dietary energy restriction, Vet. Rec., 1996, 138:354-357.) Unfortunately, the loss of lean tissue negatively impacts body condition and stamina which are required for sustained performance. The caloric density of a diet may also affect the quantity of food that must be consumed to meet energy requirements. If the metabolizable energy (ME) content of the diet is too low to support increased work, the quantity of food that must be consumed may exceed the physical capacity of the digestive tract. This may lead to increased rate of passage through the digestive tract and decreased nutrient digestibility, further exacerbating an energy deficit. The production of softer stools implies that a diet may be bulk limiting which would ultimately affect energy intake, diet digestibility and nutient availability. 17 Fat is a highly available energy source for the dog and its availability may affect performance during periods of strenuous work. Furthermore, it has been shown that dietary fat can affect body composition of exercising dogs, as lower fat diets result in a loss of lean tissue and body fat compared with higher fat diets that increase lean tissue and body fat. (Altom, E., Ph.D. Dissertation, supra.) The dog is an efficient aerobic athlete that performs best when fed a diet that supplies a large proportion of its energy as fat. (Kronfeld, D.S., Diet and the performance of racing sled dogs, J. Am. Vet. Med. Assoc., 162:470B473, 1973; Reynolds, AJ., Fuhrer, L., Dunlap, et aL., Lipid metabolite responses to diet and training in sled dogs, J. Nutr., 1994, 124:2754S-2759S; Reynolds, A.J., et al., Sled dog endurance: A result of high fat diet or selective breeding? FASEB J 1995.) Controlled studies using treadmill-exercised dogs have shown that endurance is positively correlated with dietary fat intake and diet digestibility. (Altom, E., Ph.D. Dissertation, supra; Downey RL, et al., Diet of beagles affects stamina, J. Am. Anim. Hlosp. Assoc., 1980; 16:273-277.) Furthermore, the source of dietary fat may affect hunting ability based on changes in olfactory function. Previous research has shown that olfactory sensitivity is compromised in dogs fed diets containing a greater percentage of saturated fatty acids. (Altom, E., Ph.D. Dissertation, supra) Therefore, inferior performance of hunting dogs could be attributed to the source of dietary fat used in a commercial formula. Thus, the use of beef tallow in commercial diets could negatively impact olfactory functionality and hunting performance compared with poultry fat and or fish fats because beef tallow contains a higher percentage of saturated fatty acids. The improved mammalian hunt or search methods of the present invention are enabled by diets which have a primarily poultry-based fat source, have fish as a source of particular fatty acid compounds, such as EPA and DHA, and are substantially free of beef tallow as illustrated herein. Thus, the performance enhancing diets used in the present invention have a total fat content of about 20 to about 28 percent with a mixture of saturated and unsaturated fat, and which total fat content has a lower percentage of saturated fat, such as about 23 percent, based on the total weight of the fat component compared to the Purina* Pro Plan diet (about 36 percent saturated fat) and the Diamond* diet (about 30 percent saturated fat). 18 Effects of Fat and Heat A commonly held belief among dog trainers and breeders is that feeding a high-fat diet to working dogs can predispose them to heat stress during hot weather. However, this belief was not supported by the performance data observed during the year 1 study of the present invention. The increased fat consumed by dogs fed the Eukanuba* diet compared with Diamond* diet did not negatively affect the working ability or stamina of the dogs during periods of heat stress. These results are also supported by previous research showing that a reduced fat diet produced higher rectal temperatures in dogs after one hour of treadmill exercise compared with a high fat diet. (Altom, E., Ph.D. Dissertation, supra.) Therefore, a high fat diet may be more beneficial to a working dog during periods of hot weather based on its ability to reduce core body temperature. Protein Dietary protein is also an important consideration for canine athletes. Curent evidence indicates that aerobic training imposes an increased need for dietary protein in dogs. (Hanmel, E.P., et al., Metabolic responses to exhaustive exercise in racing sled dogs fed diets containing medium, low and zero carbohydrate, Am. J. Clin. Nutr., 1976; 30:409-418; Adkins, T.O., et aL., Diet of racing sled dogs affects erythrocyte depression by stress, Can. Vet. J., 1982; 23:260-263.) In all animals, athletic conditioning results in adaptive physiological changes that facilitate efficient delivery of oxygen and nutrients to working muscles. These changes include increases in blood volume, red blood cell mass, capillary density, mitochondrial volume, and in the activity and total mass of metabolic enzymes. (Kronfcld, D.S., et al., Hernatological and metabolic responses to training in racing sled dogs fed diets containing medium, low, or zero carbohydrate, Am. J. Clin. Nutr., 1977; 30:419-430; Querengaesser, A., et al., Blood changes during training and racing in sled dogs, J. Nutr., 1994; 2760S-2764S.) The increased tissue mass and requirement for gluconeogenic amino acids during exercise necessitate increased protein intake by working dogs. The protein content of the diet may also impact the capacity of the blood to oxygenate tissue and transport energy-containing nutrients needed by working muscles. (Kronfeld, D.S., et al., supra.) Although the protein content of diets used in this study werc within the range considered to be normal for healthy dogs, a slight reduction in protein content or amino acid availability may become significant during periods of increased physical activity. 19 Thus the source of dietary protein can affect the balance and availability of amino acids that are needed by exercising tissues. It is understood by those skilled in the art of animal feeding and diet design that diet formulas arc generally not suitable for cross-feeding to other animals, for example, between dogs and cats since different animals can have family or genus specific dietary requirements or prohibitions, such as specific vitamin or mineral needs or intolerance. However, one skilled in the art recognizes these and other requirements or prohibitions and can readily reformulate basic and common nutritional components that can render them suitable for feeding to different animals. Thus the formulations of the present invention while potentially unsuitable for all animals can be readily adapted for use in other animals having similar dietary needs, physical demands, and performance objectives. The invention will now be further illustrated by the following non-limiting Example(s). The preferred mammalian species are domesticated animals such as dogs or cat. Example 1 Year 2 - Canine Rectal Temperatures The rectal temperature of each dog was obtained by a licensed veterinarian while the dog was at rest and immediately afler a 40 minute hunting session. These measurements were obtained at the end of the Year-2 hunting season (post season collection). The body temperature of the dogs was similar before hunting regardless of diet (101.7 versus 101.4 "F). The body temperature of all dogs was higher after hunting, but the increase in body temperature was greater for dogs fcd the Purina* Pro Plan diet ( 4.5 versus 3.1 "F increase in rectal temperature). The dogs fed the Purina' Pro Plan diet had significantly higher (P<0.05) body temperatures than those dogs fed the Eukanuba* diet (105.9 versus 104.8 *F, a difference of 1.1). Thus the post-hunt body temperature of the dogs fed the Eukanuba diet was from about 0.5 to about 1.5 "F lower or cooler compared to the Purina* Pro Plan diet. The lower or cooler post-hunt body temperature suggests the dogs expend less energy on.waste heat and consequently have greater endurance and greater energy available for hunt-related tasks, such as scouting and pointing. Thus, for example, the body temperature of the hunting mammal 20 fed Eukanuba* diet is maintained to within about 3.0 *F of the pre-hunt body temperature which is significantly lower than hunting mammals fed Purina* Pro Plan diet and as summarized in the accompanying Table. Canine Rectal Temperature (* Fahrenheit) at Post-Season Collections Eukanubag Purina* P(< Pre-hunt 101.7 101.4 NS Post-hunt 104.81 105.91 0.05 Difference 3.1 4.5 1. Probability or confidence lcvcl greater than 95%. All publications, patents, and patent documents are incorporated by reference herein individually in their entirety. The invention has been described with reference to various specific and preferred embodiments and techniques. However, it should be understood that many variations and modifications may be made while remaining within the spirit and scope of the invention. 21

Claims (48)

1. A method for increasing the hunt performance of a hunting dog which comprises orally administering to the dog an effective amount of a diet comprising unsaturated fatty acid 5 EPA, unsaturated fatty acid DHA, or both, in a total amount of the diet greater than about 0.2 weight percent; and wherein the diet comprises a total fat content from about 20 to about 28 weight percent and greater than about 70 weight percent of the total fat content of the diet is unsaturated fat and wherein the diet further comprises a saturated fat. 10

2. The method of claim 1 wherein the diet comprises said total fat content from about 20 to about 25 weight percent.

3. The method of claim I wherein the total fat content has greater than about 75 weight 15 percent unsaturated fat.

4. The method of claim I wherein the total fat content has greater than about 85 weight percent unsaturated fat. 20

5. The method of claim I wherein the diet further comprises a total protein content of about 30 to about 35 weight percent, and a total carbohydrate content of about 30 to about 35 weight percent.

6. The method of claim 1 wherein the unsaturated fat is from fish meal, chicken fat, and 25 flax meal.

7. The method of claim I wherein the diet is free of beef fat.

8. A method for increasing the heat endurance of a hunting dog which comprises orally 30 administering to the dog an effective amount of a diet comprising unsaturated fatty acid EPA, unsaturated fatty acid DHA, or both, in a total amount of the diet greater than about 0.20 weight percent; and wherein the diet comprises a total fat content from about 20 to about 28 weight percent and greater than about 70 weight percent.of the 22 809403 20090)202 Spei total fat content of the diet is unsaturated fat and wherein the diet further comprises a saturated fat.

9. The method of claim 8 wherein the diet comprises said total fat content from about 20 5 to about 25 weight percent.

10. The method of claim 8 wherein the total fat content has greater than about 75 weight percent unsaturated fat. 10

11. The method of claim 8 wherein the total fat content has greater than about 85 weight percent unsaturated fat.

12. The method of claim 8 wherein the diet further comprises a total protein content of about 30 to about 35 weight percent, and a total carbohydrate content of about 30 to 1s about 35 weight percent.

13. The method of claim 9 wherein the unsaturated fat is from fish meal, chicken fat, and flax meal. 20

14. The method of claim 8 wherein the diet is free of beef fat.

15. A method for maintaining the body temperature of a hunting dog which comprises orally administering to the dog an effective amount of a diet comprising unsaturated fatty acid EPA, unsaturated fatty acid DHA, or both, in a total amount of the diet of 25 greater than about 0.20 weight percent; and wherein the diet comprises a total fat content from about 20 to about 28 weight percent and greater than about 70 weight percent of the total fat content of the diet is unsaturated fat and wherein the diet further comprises a saturated fat. 30

16. The method of claim 15 wherein the diet comprises said total fat content from about 20 to about 25 weight percent.

17. The method of claim 15 wherein the total fat content has greater than about 80 weight percent unsaturated fat. 23 KI9403 200)902112 Sp-i

18. The method of claim 15 wherein the diet further comprises a total protein content of about 30 to about 35 weight percent, and a total carbohydrate content of about 30 to about 35 weight percent. 5

19. The method of claim 15 wherein the unsaturated fat is from fish meal, chicken fat, and flax meal.

20. The method of claim 15 wherein the diet is free of beef fat. 10

21. The method of claim 15 wherein the diet provides greater heat endurance to the hunting dog during hunting compared to a diet having a lower EPA and DHA total weight percent. 15

22. The method of claim 15 wherein the hunting dog is an English Pointer.

23. A method for increasing the hunt performance of a dog which comprises orally administering to the dog for an effective time and in an effective amount a diet comprising unsaturated fatty acid EPA, unsaturated fatty acid DHA, or both, in a total 20 amount of the diet greater than about 0.20 weight percent; and wherein the diet comprises a total fat content from about 20 to about 28 weight percent and greater than about 70 weight percent of the total fat content of the diet is unsaturated fat and wherein the diet further comprises a saturated fat. 25

24. The method of claim 23 wherein the diet comprises said total fat content from about 20 to about 25 weight percent.

25. The method of claim 23 wherein the unsaturated fat content is from fish meal, chicken fat, and flax meal. 30

26. The method of claim 24 wherein the diet is free of beef fat, beef protein, or both.

27. The method according to claim 23 wherein the effective time is a period of from about I to about 24 months. 24 809403 2WA202 Spmi

28. The method according to claim I wherein the method provides an increase in target finds in an amount of from about 30 to about 35 percent per hunt session. 5

29. The method according to claim I wherein the method provides an increase in target finds of about 33 percent per hunt session over an entire hunting season.

30. The method of claim I wherein the hunt performance is increased by from about 1 to about 2 more target finds per hour compared to a dog fed a diet having a lower weight 10 percent of unsaturated fatty acid EPA, unsaturated fatty acid DHA, or both.

31. The method according to claim 30 wherein the target is an animal or search object.

32. The method according to claim 30 wherein the target is an upland game bird, a 15 swimming game bird, or a shorebird.

33. The method of claim I wherein the hunting dog's body composition is maintained during the hunting season. 20

34. The method of claim I wherein the hunting dog's appetite is maintained during the hunting season.

35. The method according to claim I wherein the administering is accomplished by feeding the diet once-a-day during training season, physical conditioning season, or 25 hunting season.

36. The method according to claim I wherein a normal blood chemistry profile is maintained in the dog. 30

37. The method according to claim I wherein the administering uses about 2/3 of a cup less food each day to maintain body weight and body condition during the hunting season compared to a diet having a lower weight percentage of EPA, DHA, or both. 25 809403 2(0)22 Spi

38. The method according to claim I wherein the weight ratio of EPA to DHA in the diet is from about 0.8:1.5 to about 1.5:0.7.

39. The method according to claim I wherein the weight ratio of EPA to DHA in the diet is 5 from about 1.0:1.5 to about 1.5:1.0.

40. The method according to claim 1 wherein the diet further comprises the unsaturated fatty acid DPA in an amount of from 0.018 to about 0.034 weight percent based on the total weight of the diet. 10

41. The method according to claim 8 wherein the diet further comprises the unsaturated fatty acid DPA in an amount of from 0.018 to about 0.034 weight percent based on the total weight of the diet. 15

42. The method according to claim 8 wherein the diet has unsaturated fatty acids EPA, DPA, and DHA in a total amount of from about 0.45 to about 0.60 weight percent.

43. The method according to claim 8 wherein the diet has a total amount of unsaturated fatty acids EPA, DPA and DHA of about 0.50 to about 0.55 weight percent. 20

44. The method according to claim 15 wherein the diet further comprises the unsaturated fatty acid DPA in an amount of from 0.018 to about 0.034 weight percent based on the total weight of the diet. 25

45. The method according to claim 23 wherein the diet further comprises the unsaturated fatty acid DPA in an amount of from 0.018 to about 0.034 weight percent based-on the total weight of the diet.

46. The method according to claim I wherein the total amount of EPA and DHA is from 30 about 0.21 to about 0.30 weight percent.

47. The method according to claim 1 wherein the total amount of EPA and DHA is from about 0.22 to about 0.28 weight percent. 26 XI$)403 2(1202 Spcci

48. The method according to claim I wherein the diet further comprises omega-6 fatty acid and omega-3 fatty acid in a weight ratio of about 5:1 to about 10:1. 27 8(09403 2(X)90202 Spomi