AU2007200680A1 - 5-phenyl-4-methyl-thiazol-2-yl-amine derivatives as inhibitors of phosphatidylinositol 3 kinase enzymes (pi3) for the treatment of inflammatory airway diseases - Google Patents

Description

P/00/011 Regulation 3.2

AUSTRALIA

Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT

(ORIGINAL)

Name of Applicant: Actual Inventors: Novartis AG, of Lichtstrasse 35, CH-4056 Basel, Switzerland Bloomfield, Graham Charles Bruce, Ian Hayler, Judy Fox Le Grand, Darren Mark Leblanc, Catherine McCarthy, Clive DAVIES COLLISON CAVE, Patent Trademark Attorneys, of 1 Nicholson Street, Melbourne, 3000, Victoria, Australia Ph: 03 9254 2777 Fax: 03 9254 2770 Attorney Code: DM "5-phenyl-4-methyl-thiazol-2-yl-amine derivatives as inhibitors of phosphatidylinositol 3 kinase enzymes (pi3) for the treatment of inflammatory airway diseases" Address for Service: Invention Title: The following statement is a full description of this invention, including the best method of performing it known to us:- PAOPERASU3O217S div daimid-~9M22l07 ORGANIC COMPOUNDS This is a divisional of Australian patent application No. 2004268050, the entire contents of which are incorporated herein by reference.

The present invention relates to organic compounds, their preparation and their use as 00 5' pharmaceuticals.

In a first aspect, the present invention provides compounds of formula I

RI

C-N

R 3 1 b in free or salt form, wherein RS- is hydrogen or Cl-C4-alkyl, Rb is Gi-Cs-alkyl substituted by pyridyl, R 3 is R6, and R 4 is fluoro or Gi-Ce-haloalkyl, or R- is hydrogen or Cl-C.,-alkyl, Rb is Ci-Cs-alkyl substituted by hydroxy or nitrile, R 3 is R6, and R 4 is hydrogen or Ci-Cs-haloalkyl, or Ra is hydrogen or Cl-C 4 -alkyl, R b is Ci-Cs-alkyl substituted by nitrile, R 3 is fluoro, and R 4 is or Ra is hydrogen or Ct-q-alkyl, Rb is Ci-Ce-alkyl substituted by hydroxy, R 3 is fluoro, and R 4 is R 7 or R- is hydrogen or CI-C4-alkyl, Rb is CI-Cs-alkyl substituted by di(CI-Ca-alkyl)amino, R3 is R6, and R 4 is Ci-Ce-haloalkyl, or R, is hydrogen or Ci-Cq-alkyl, Rb is Ci-Cs-alkyl substituted by -O-Ci-Cs-alkyl-OH, R 3 is

R

6 and R 4 is fluoro or Gi-Cs-haloalkyl, or R- is hydrogen or Cz-Cs-alkyl, R6 is -CH(CH 3 )-CFL-OH, R 3 is and R 4 is fluoro, or R- is hydrogen or CI-C4-alkyl, Rb is Ci-Ca-alkyl substituted by pyrrolidinyl substituted by C2-Ca-alkyl, RWis R6, and R 4 is Ci-Ca-haloalkyl, or R" is hydrogen or Ci-C4-alkyl, Rb is CI-Cs-alkyl substituted by oxazolyl substituted by Ci-CB-alkyl, RI is R6, and R 4 is nitrile or imidazolyl, or Re is hydrogen or Ci-CQ-alkyl, Rb is Ci-Cs-alkyl substituted by imidazolyl, RI is R 6 and R 4 is fluoro, 00 IND or R, is hydrogen or CI-C4-alkyl, Rb is Cl-Ca-alkyl substituted by benzoimidazoiyl,

R

3 is R 6 and R 4 is fluoro, or R, is hydrogen or Cl-C 4 -alkyl, R b is CI-Cs-alkyl substituted by isoxazolyl substituted by r-K1CI-Ce-alkyl,

R

3 is R 6 and R 4 is R 7 or R- is hydrogen or CI-C4-alkyl, Rb is Ci-Ce-alkyl substituted by pyrrolyl substituted by Ci-Ce-alky], RI is and R 4 is R 7 or R- is hydrogen or CI-C 4 -alkyl, R6 is CI-Cs-alkyl substituted by pyrazolyl substituted by Cl-Co-alkyl,

R

3 is R6, and R 4 is R 7 or Re is hydrogen or CI-C 4 -alkyl, Rb is Cl-Ce-alkyl substituted by -CO-O-C- 3 -CO-O-butyl, -CO-di(CI-Ce-alkyl)axnino,

-CO-NH

2 -NH-CO-Cl-Ca-alkyl, -SOz-Ci-Co-alkyl, -CO-N1I-Rc where Rc is napthyl, or by -CO-NH-Ci-C8-alkyl optionally substituted by di(Ci-Cs-alkyl)amino, R 3 is R 6 and R 4 isR7 or R- is hydrogen or CI-Cq-alkyl, R6 is -CH(CH,4-CO-NH-Cj-Cs-akl or -CH(CH3)-CO-O-Ci-Cs-alkyl,

R

3 is R6, and R 4 is R 7 or Ra is hydrogen or CI-C4-alkyl, Rb is Ci-Cs-alkyl substituted by -CH(OH)-CH2-OH,

R

3 is R6 and R 4 is R 7 or RA is hydrogen or CI-C4-alkyl, Rb is Ci-Ce-alcyl substituted by Ci-Cs-alkoxy, or by -:S-Cl-CBalkyl, R3 is R6, and R 4 is R 7 or R- is hydrogen or Ci-Ci-alkyl, Rb is Ci-Ca-alkyl substituted by a 5- or 6-membered heterocyclic ring having one or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, that ring being substituted by oxo, R 3 is R6, and R 4 is R7, or R- is hydrogen or Cl-C4i-alkyl, Rb is Ct-C 8 -alkyl substituted by a 5- or 6-membered heterocyclic*ring having three or more ring herero atoms selected from the group consisting of IND oxygen, nitrogen and sulphur, that ring being optionally substituted by CI-CS-alkyl, -Cl-Ca-alkyl-di(Ci-C8-alkyl)ariino, or by C 3 -Ca-cycloalkyl, R 3 is R6, and R 4 is R 7 00 or Ra is hydrogen or Ci-C.4-alkyl, Rb is Cl-Co-alkyl substituted by oxazolyl substituted by C3-Ca-alkyl, R 3 is R 6 and R 4 is R7, or R- is hydrogen or CI-C 4 -alkyl, R b is Cl-Co-alkyl substituted by imidazolyl substituted by CI-CB-alkyl optionally substituted by hydroxy or Ci-Ce-alkoxy, R 3 is R6, and R4 is R 7 or Ra is hydrogen or Ci-Cv-alkyl, Rb is Ci-Os-alkyl substituted by -CO-Het where Het is a 5- or 6-membered heterocyclic ring having two or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, that ring being optionally substituted by Ci-Cs-alkyl, R 3 is R 6 and R4~ is R 7 or R-I is hydrogen or CI-C4t-alkyl, Rb is a 5- or 6-membered heterocyclic ring having one or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, that ring being substituted by oxo, R 3 is R6, and R 4 is R 7 or RB is hydrogen or CI-C4-alkyl, R b is an aza-bicyclo[3.2.I]oct-3-yl ring optionally substituted by Cl-Cs-alkyl, RI is R 6 and R(4 is R 7 or R-1 and Rb together form an azetidine ring substituted by Ci-Ce-alkoxycarbonyl or nitrile, R 3 is R 6 and R 4 is R 7 or RB and Rb together form a pyrrolidine ring substituted by -CO-NH 2 or nitrile, R 3 is R 6 and R4 is R 7 or R, and Rb together form an imidazo-pyridine ring, R3 is R6, and R 4 is R.7; RI is Cl-C 4 -alkyl or halogen; RI is hydrogen, halogen or Ci-Ca-alkyl;

R

6 is halo, -S0 2

-CH

3

-SO

2 -CF3, carboxy, -CO-NH 2 -CO-di(Ci-Cs-alkyl)amino, or a 5- or 6membered heterocyclic ring having one or more ring hetero atoms selected from the group IN consisting of oxygen, nitrogen and sulphur, that ring being optionally substituted by halo, cyano, oxo, hydroxy, carboxy, nitro, C3-CS-cycloalkyl, Cl-GC-alkylcarbonyl, Ci-Cs-alkoxy optionally substituted by aminocarbonyl, or Ci-C-alkyl optionally substituted by hydroxy, Ci- 00 Cs-alkoxy, C1-Cs-alkylamino or di(CI-Cs-alkyl)amino;

\O

R' is hydrogen, halo, -SO2-CH, nitrile, Ci-Cs-haloalkyl, imidazolyl, Ci-Cs-alkyl, -NROR', or -SOz-NR 8 and R8 and R' are independently hydrogen, amino, Ci-Ce-alkylamino, di(Ci-Co-alkyl)amino, or C 1 Cs-alkyl optionally substituted by hydroxy, or R3 and R' together form a 5- to 10-membered heterocyclic ring having one or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, that ring being optionally substituted by halo, cyano, oxo, hydroxy, carboxy, nitro, C3-Cs-cycloalkyl, Ci-Ca-alkylcarbonyl, CI-C-alkoxy optionally substituted by aminocarbonyl, or Ci-Cs-alkyl optionally substituted by hydroxy, CI-Cs-alkoxy, Cl-Cs-alkylamino or di(Cl-Cs-alkyl)amino.

Terms used in the specification have the following meanings: "Substituted" as used herein means the group referred to is substituted at one or more positions by any one or any combination of the radicals listed thereafter.

"Optionally substituted" as used herein means the group referred to can be substituted at one or more positions by any one or any combination of the radicals listed thereafter.

"Halogen" or "halo" may be fluorine, chlorine, bromine or iodine. Preferably halogen or halo is fluorine or chlorine.

"C-Cs-alkyl" denotes straight chain or branched CI-Cs-alkyl. Preferably, Ci-Ce-alkyl is C 1

-C

4 alkyl.

"Ci-Cs-alkoxy" denotes straight chain or branched Ci-Cs-alkoxy. Preferably, Ci-Cs-alkoxy is CI-C4-alkoxy.

'G3-Cs-cycloalkyl denotes cycloalkyl having 3 to 8 ring carbon atoms, for example a monocyclic group such as a cyclopropyl, cyclobutyl, cyclopenryl, cyclohexyl, cycloheptyl or IND cyclooctyl, any of which can be substituted by one or more, usually one or two, Ci.-C 4 -alkyl grou'ps, or a bicyclic group such as bicycloheptyl or bicycloocryl. Preferably, "C3-C8cycloalkyl" is C3-Cs-cycloalkyl, especially cyclopropyl or cyclobutyl.

00 Ci -Cs-haloalkyl denotes Ci-Cs-alkcyl as hereinbefore defined substituted by one or more c-i halogen atoms as hereinbcfore defined. Preferably, Cl-Cs-haloalkyl is Ci-Ci-alkyl substituted by one, two or three fluorine or chlorine atoms, especially -CF 3 "Aminocarbonyl" as used herein denotes amino attached through the nitrogen atom to a carbonyl group.

'Ci-Co-alkylcarbonyl" and "Ci-Co-alkoxycarbonyl" denote Ci-Cs-alkyl and Cl-Cs-alkoxy respectively as hereinbefore defined attached by a carbon atom to a carbonyl group.

Preferably, Ci-C8-alkylcarbonyl and Cl-C8-alkoxycarbonyl are respectively C 1

-C

1 alkylcarbonyl and C2-C4-alkoxycarbonyl.

Ci-Coalkyl amino" and udi(CI-Cs-alkiyl)aminoP as used herein denote amino substituted respectively by one or two CI-Cs-alkyl groups as hereinbefore defined, which may be the same or different, Preferably, Ci-Co-alkylamino and di(CI-Ce-alkyl)amino are respectively C 1

-C

4 alkylamino and di(Cl-C4-alkyl)arnino.

or 6-membered heterocyclic ring containing at least one ring heteroatom selected from the group consisting of nitrogen, oxygen and sulphur" as used herein may be, for example, furan, pyrrole, pyrrolidine, pyrazole, imidazole, triazole, isotriazole, tetrazole, thiadiazole, isothiazole, oxadiazole, pyridine, oxazole, isoxazole, pyrazine, pyridaziae, pyrimidine, piperazine, morpholino, triazine, oxazine or thiazole. Preferred heterocyclic rings include piperazine, morpholino, imidazole, isoti-iazole, pyrazole, pyridine, furan, oxazole, isoxazole, oxadiazole and azetidine. The 5- or G-membered heterocyclic ring can be unsubstituted or substituted.

Preferred substituents include halo, cyano, oxo, hydroxy, carboxy, nitro, C3-CS-cyclOalkyl, C 1 Ca-alkylcarbonyl, Ci-Ca-alkoxy optionally substituted by aminocarbonyl, and CI-Ca-alkyl optionally substituted by hydroxy, Ci-Ca-alkoxy or di(CI-Cs-alkyl) amino. Especially preferred substituents include halo, oxo, C3-C.S-cycloalkyl, and CI-C4-alkyl optionally substituted by hydroxy, Cl-C4-alkoxy, Ci-Cs-alkylamino or di(Cl-C 4 -alkyl)amino.

to 1 0-membered heterocyclic ring having one or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur" as used herein may be, for example Preferred 5- to 10-membered heterocyclic rings include morpholino, piperazinyl and imidazolyl. The 5- to 10-membered heterocyclic ring can be unsubstituted or substituted.

Preferred substituents include halo, cyano, oxo, hydroxy, carboxy, nitro, Ca-C-cycloalkyl,

C

1 0 Ce-alkylcarbonyl, Cl-Cs-alkoxy optionally substituted by aminocarbonyl, or C-Cs-alkyl optionally substituted by hydroxy, Ci-Co-alkoxy, Ci-Cs-alkylamino or di(Ci-Cs-alkyl)amino.

Especially preferred substituents include CI-C4-alkyl.

Throughout this specification and in the claims that follow, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.

Preferred compounds of the present invention include compounds of formula I in free or salt form, wherein R, is hydrogen, Rb is CI-Cs-alkyl substituted by pyridyl, R 3 is and R 4 is fluoro or CI-Cshaloalkyl, or R- is hydrogen, Rb is Ci-Cs-alkyl substituted by hydroxy or nitrile, R3 is and R 4 is hydrogen or Ci-Co-haloalkyl, or R' is hydrogen, Rb is Ci-Ce-alkyl substituted by nitrile, R 3 is fluoro, and R4 is R 7 or R is hydrogen, Rb is Ci-Ci-alkyl substituted by hydroxy,

R

3 is fluoro, and R4 is R 7 or R' is hydrogen, Rb is Ci-Cs-alkyl substituted by di(Ci-Cs-alkyl)amino,

R

3 is and R 4 is C 1 Cs-haloalkyl, or Ra is hydrogen, Rb is Ci-Cs-alkyl substituted by -O-Ci-Cs-alkyl-OH,

R

3 is and R 4 is fluoro or Ci-Cs-haloalkyl, or Ra is hydrogen, Rb is -CH(CH 3 )-CH2,OH,

R

3 is and R 4 is fluoro, or Ra is hydrogen, Rb is C1-Cs-alkyl substituted by pyrrolidinyl substituted by C1-Ca-alkyl,

R

3 is Rd, and R 4 is Ci-Cs-haloalkyl, or R- is hydrogen, Rb is Ci-C-alkyl substituted by oxazolyl substituted by CI-Cs-alkyl,

R

3 is and R 4 is nitrile or imidazolyl, or RI is hydrogen, Rb is C1-Ca-alkyl substituted by imidazolyl,

R

3 is R 6 and R4 is fluoro, or Ra is hydrogen, Rb is Ci-Ca-alkyl substituted by benzoimidazolyl,

R

3 is R 6 and R 4 is fluoro, or R' is hydrogen, Rb is C1-C8-alkyl substituted by isoxazolyl substituted by CI-CO-alkyl,

R

3 is and R4 is R',

S.

7 or Re is hydrogen, Rb is Ci-Cs-alkyl substituted by pyrrolyl substituted by Ci-Ci-alkyl, R 3 is R6, and R 4 is R7, IND or Re is hydrogen; Rb is CI-Ce-alkyl substituted by pyrazolyl substituted by Ci-CO-alkyl, R 3 is R6, and RI is R(7, or Re is hydrogen, Rb is Ci-Cs-alkyl substituted by -CO-O-CH 3 -CO-O-butyl, -CO-di(C 1 -Co.

00 alkyl)amino, -CO-NH 2 -NH-CO-Ci-Co-alkyl, -S02-Cl-Co-alkyl, -CO-NH-Rc where 1(c is* napthyl, or by -CO-NH-Cl-Cit-alkyl optionally substituted by di(CI-Ce-alkyl)amiino, R 3 is R(6, and R 4 is R7, or Re is hydrogen, Rb is -CH(CH 3 )-CO-NH-Ci-Cs.alky or -CH(CH 3 )-GO-O-Cj-Cs-alk-yl, R(3 is R(6, and R 4 is R', or Re is hydrogen, Rb is Ci-Ce-alkyl substituted by -CH(OH)-CH2z-OH, R 3 is R(6, and R4 is R 7 or ROis hydrogen, Rb is Ci-Cs-alkyl substituted by Cl-Co-alkoxy, or by -S-Ci-Co-alkyl, R(3 is R6, and R(4 is R 7 or Re is hydrogen, 1(b is CI-Cs-alkyl substituted by a 5- or 6-membered heterocyclic ring having one or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, that ring being substituted by oxo, R 3 is R(6, and R 4 is R(7, or Re is hydrogen, 1(b is Ci-Cs-alkyl substituted by a 5- or 6-membered hetcrocycic ring having three or more ring hetero, atoms selected from the grou p consisting of oxygen, nitrogen and sulphur, that ring being optionally substituted by CI-Ca-alkyl, -Cl-Ca-alkyl-di(Cx-Csalkyl)amino, or by G3-Gs-cycloalkyl, R(3 is R6, and R 4 is R 7 or Re is hydrogen, 1(b is Cl-Cs-alkyl substituted by oxazolyl substituted by C3-Cs-alkyl, R(3 is R(6, and R4 is R(7, or Re is hydrogen, 1(b is Ci-Cs-alkyl substituted by imidazolyl substituted by Ci-Ce-alkyl optionally substituted by hydroxy or Ci-Co-alkoxy, R3 is R(6, and R(4 is R(7, or Re is hydrogen, Rb is Ci-Co-alkyl substituted by -CO-Het where Her is a 5- or 6-membered heterocyclic ring having two or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, that ring being optionally substituted by Cl-Co-alkyl, R(3 is R6, and R(4 is R 7 or R(e is hydrogen, 1(b is a 5- or 6-membered heterocyclic ring having one or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, that ring being substituted by oxo, R(3 is R(6, and R 4 is R(7, or R(e is hydrogen, Rb is an aza-bicyclo[3.2.1]oct-3-yl ring optionally substituted by Ci-Cs-alkyl, R 3 is R6, and R 4 is R 7 or R(e -and 1(b together form an azetidine ring substituted by Ci-Ca-alkoxycarbonyl or nitrile, R3 is R(6, and R(4 is R(7, or Re and R6 together form a pyrrolidine ring substituted by -CO-NI- 2 or nitrile, R 3 is R6, and

R

4 isR 7 INDor Re and Rb together form an irnidazo-pyridine ring, RI is R6, and R 4 is R 7

R

2 is CI-C 4 -alkyl or halogen;

R

5 is hydrogen; 00 R -is halo or -SO2-Cl-b; and IND RI is hydrogen, halo, -S0 2

-CH

3 nitrile, Ci-Co-haloalkyl or irnidazolyl.

Especially preferred compounds of the present invention include compounds of formula I in free or salt form, wherein C1Re is hydrogen, Rb is Ci-C4-alkyl substituted by pyridyl, R 3 is R6, and R 4 is fluoro or C-4 haloalkyl, or Re is hydrogen, Rb is C1-C4-alkyl substituted by hydroxy or nitrile, R 3 is R6, and R 4 is hydrogen or Ci-C4s-haloalkyl, or Ra is hydrogen, Rb is CI-C4s-alkyl substituted by nitrile, R 3 is fluoro, and R4~ is R 7 or Re is hydrogen, Rb is Cl-C 4 -alkyl substituted by hydroxy, R 3 is fluoro, and R 4 is R 7 or Re is hydrogen, Rb is Ci-C4-alkyl substituted by di(C1-C4-alkyl)amino, R 3 is R6, and R 4 is Cj- G-haloalkyl, or Re is hydrogen, Rb is Ci-C4-alkyl substituted by -O-G1-C4-alkyl-OI-, R 3 is R 6 and R 4 is fluoro or Ct-C4-haloalkyl, or Re is hydrogen, Rb is -CH(CH3)-CH 2

R

3 is R6, and R4 is fluoro, or Re is hydrogen, Rb is Ci-C4s-alkyl substituted by pyrrolidinyl substituted by CI-C4i-alkyl, R 3 is R6, and R 4 is Cl-C4-haloalkyl, or Re is hydrogen, Rb is CI-C 4 -alkyl substituted by oxazolyl. substituted by CI-Ci-alkyl, R 3 is R6, and R4 is nitrile or iinidazolyl, or Ra is hydrogen, Rb is Ci-C 4 -alkyl substituted by imidazolyl 1

R

3 is R6, and R4 is fluoro, or Re is hydrogen, Rb is CI-C4-alkyl substituted by benzoimidazolyl, R 3 is R6, and R4 is fluoro, or Re is hydrogen, Rb is Ci.-Ci-alkyl substituted by isoxazolyl substituted by CI-C4-alkyl, R 3 is R6, and R.

4 is R 7 or Re is hydrogen, Rb is Cl-C4-alky! substituted by pyrrolyl substituted by Ci-CQ-alkyl, R 3 is R6, and R! is R 7 or R.S is hydrogen, RI is CI-C4-alkyl substituted by pyrazolyl substituted by CI-C4s-alkyl, R.

3 is

R

6 and R4 is R 7 or Re is hydrogen, Rb is CI-C 4 -alkyl substituted by -CO-O-CH 3 -CO-O-butyl, -CO-di(Cl-C4alkyl)amino, -CO-NH 2 -NH-CO-Cl-C4-alkyl, -S02-CI-C4-alkyl, -CO-NH-P.' where Rc is napthyl, or by -CO-NH-Ci-C 4 -alkyl optionally substituted by di(Ci-C 4 alkyl)amino, R 3 is R6, and R 4 is R7, or RB is hydrogen, R 6 is -CH(CH.

3 )-CO-NII-Ci-C 4 -alkyl or -CH(CI{3)-CO-O-CI-C 4 -alkyl, R 3 is R6, and R 4 is R7, or Re is hydrogen, Rb is GI-C 4 -alkyl substituted by -CH(OH)-GH 2 -OH, R 3 is R 6 and R 4 is R 7 00 or Re is hydrogen, Rb is CI-C4-alkyl substituted by Ci-Ce-alkoxy, or by -S-Cl-CQ-alkyl, R 3 is R6, and R 4 is R 7 or Re is hydrogen, Rb is G1-C4-alkyl substituted by a S- or G-membered heterocyclic ring having one or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, that ring being substituted by oxo, R 3 is R 6 and R 4 is R 7 or Re is hydrogen, Rb is CI-C4-alkyl substituted by a 5- or 6-membered heterocyclic ring having three or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, that ring being optionally substituted by C2-Co-alkl, -Ci-Cs-alkyl-dli(Ci-C4i-alkyl)amino, or by C3-Cs-cycloalkyl,

R

3 is R6, and R 4 is R7, or Re is hydrogen, Rb, is CI-C 4 -alkyl substituted by oxazolyl substituted by C3-CS-alkyl, R 3 is

R

6 and R 4 is R7, or Re is hydrogen, Rb is CI-C 4 -alkyl substituted by imidazolyl substituted by CI-C.,-alkyl optionally substituted by hydroxy or Ci-C4-alkoxy, R 3 is R6, and R 4 is R 7 or Re is hydrogen, Rb is CI-C 4 -alkyl substituted by -CO-Het where Het is a 5- or 6-membered heterocyclic ring having two or more zing hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, that ring being optionally substituted by CI-Ci4-alkyl, R 3 is R6, and R 4 is R 7 or Re is hydrogen, Rb is a 5- or 6-membered heterocyclic ring having one or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, that ring being substituted by oxo, R 3 is R 6 and R 4 is R 7 or Re is hydrogen, Rb is an aza-bicyclo[3.2.I)oct-3-yl ring optionally substituted by Ci-Q-alkyl, RI is and R 4 is R 7 or Re and Rb together form an azetidine ring substituted by CI-C4s-alkoxycarboiyl or nitrile, R3 is R5, and R 4 is R 7 or R, and Rb together form a pyrrolidine ring substituted by -CO-NI- 2 or nitrile, R 3 is R6. and

R

4 is R 7 or RB and Rb together form an imidazo-pyridine ring, R 3 is R6, and R 4 is R7;

R

2 is CI-C 4 -alkyl or halogen;

R

5 is hydrogen; R6 is halo or -SOz-GH, 3 and R7 is hydrogen, halo, -SO2-CH 3 nitrile, Ci-C4t-haloalkyl or imidazolyl.

Many of the compounds represented by formula I are capable of forming acid addition salts, Sparticularly pharmaceutically acceptable acid addition salts. Pharmaceutically acceptable acid IND addition salts of the compound of formula I include those of inorganic acids, for example, hydrohalic acids such as hydrofluoric acid, hydrochloric acid, hydrobromic acid or hydroiodic acid, nitric acid, sulfuric acid, phosphoric acid; and organic acids, for example aliphatic 00 monocarboxylic acids such as formic acid, acetic acid, trifluoroacetic acid, propionic acid and I butyric acid, aliphatic hydroxy acids such as lactic acid, citric acid, tartaric acid or malic acid, Sdicarboxylic acids such as maleic acid or succinic acid, aromatic carboxylic acids such as Sbenzoic acid, p-chlorobenzoic acid, diphenylacetic acid or triphenylacetic acid, aromatic hydroxy acids such as o-hydroxybenzoic acid, p-hydroxybenzoic acid, 1-hydroxynaphthalene- 2-carboxylic acid or 3-hydroxynaphthalene-2-carboxylic acid, and sulfonic acids such as methanesulfonic acid or benzenesulfonic acid. These salts may be prepared from compounds of formula I by known salt-forming procedures.

Compounds of formula I which contain acidic, e.g. carboxyl, groups, are also capable of forming salts with bases, in particular pharmaceutically acceptable bases such as those well known in the art; suitable such salts include metal salts, particularly alkali metal or alkaline earth metal salts such as sodium, potassium, magnesium or calcium salts, or salts with ammonia or pharmaceutically acceptable organic amines or heterocyclic bases such as ethanolamines, benzylamines or pyridine. These salts may be prepared from compounds of formula I by known salt-forming procedures.

Specific preferred compounds of formula I are described hereinafter in the Examples.

In a second aspect, the present invention provides a process for preparing a compound of formula I in free or salt form wherein R 2

R

3

R

4 RS, Ra and Rb are as hereinbefore defined, which process comprises the steps of: reacting a compound of formula II RN H S C-T II wherein R 2

R

3

R

4 and R s are hereinbefore defined and T is a 5- or 6-membered heterocyclic ring having one or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, with a compound of formula III Ra

RB

H-N III Rb wherein R and Rb are hereinbefore defined; reacting compounds of formula IV wherein R 2

R

3

R

4 and R 5 are hereinbefore defined with a compound of formula i wherein R and Rb are hereinbefore defined; for the preparation of compounds of formula I where RA is hydrogen and R 2

R

3

R

4 RS and Rb are as hereinbefore defined, reacting a compound of formula V wherein R 2

R

3

R

4 and Rs are hereinbefore defined, with a compound of formula VI Rb-N=C=O VI wherein Rb is as hereinbefore defined; or for the preparation of compounds of formula I where R- is hydrogen, Rb is C1-Ce-alkyl substituted by imidazolyl substituted by Ci-Ca-alkyl optionally substituted by hydroxy or Ci-C-alkoxy and R 2

R

3

R

4 and R 5 are as hereinbefore defined, reacting a compound of formula V where R 2

R

3

R

4 and R J are hereinbefore defined, with a compound of formula VI S12 SB Br- IN HN N- S1N-Q VII where Q is Ci-Cs-alkyl optionally substituted by hydroxy or Ci-Cs-alkoxy; and 00 O0 0 (ii) recovering the resultarit compound of formula I in free or salt form.

0 Process variant may be carried out using known procedures for reacting carbonyl di- O heterocyclic intermediates acylimidazolides when T is imidazole) with amines to form ureas, or analogously, e.g. as hereinafter described in the Examples. The reaction may be carried out in an organic solvent, e.g. dimethylformamide (DMF) or dioxane, in the presence or absence of a base, for example triethylamine or sodium hydride. The reaction temperature may be from about 100 C to about 1000 C, but conveniently room temperature.

Process variant may be carried out using known procedures for reacting isocyanates with amines, or analogously, e.g. as hereinafter described in the Examples. The reaction may be carried out in an organic solvent, e.g. dioxane or DMF. The reaction temperature may be an elevated temperature, for example from 500 C to 1000 C, but preferably about 80° C.

Process variants may be carried out using known procedures for reacting isocyanates with amines, or analogously, e.g. as hereinafter described in the Examples. The reaction may be carried out in an organic solvent, e.g. dioxane or DMF. The reaction temperature may be an elevated temperature, for example from 500 C to 1000 C, but preferably about 800 C.

Process variant may be carried out using known procedures for reacting 2-alkyl-S-oxo- 5,6,7,8-tetrahydro-imidazo[1,S-c]pyrimidin-2-ium compounds with 2-amino-S-phenylthiazoles, or analogously, e.g. as hereinafter described in the Examples. The reaction may be carried out in an organic solvent, e.g. DMF, in the presence of a base, e.g. triethylamine. The reaction temperature may be 100-1700 C, but conveniently about 1200 C.

Compounds of formula II or formula IV may be prepared by reacting a compound of formula V wherein R 2

R

3

R

4 and Rs are as hereinbefore defined with a compound of formula VIII T

VIII

0 WO 2005/021519 PCT/EP2004/009586 13 wherein each T, which may be the same or different, is a 5- or 6-membered heterocyclic ring Shaving one or more ring hetero atoms selected from the group consisting of oxygen, nitrogen IND and sulphur, using known procedures, or analogously, e.g. as described in the Examples. The compound of formula VII is preferably 1,1'-carbonyldiimidazole (CDI). The reaction may be O carried out in an organic solvent, e.g. dichloromethane (DCM). The reaction temperature may 00 be from 200 C to the reflux temperature of the solvent, but conveniently about 400 C.

O

Compounds of formula IV may also be prepared by reacting a compound of formula V Swherein R 2

R

3

R

4 and Rs are as hereinbefore defined, with phosgene, using known O procedures, or analogously, e.g. as described in the Examples.

Compounds of formula II are commercially available or may be prepared by known methods, or analogously, e.g. as described in the Examples.

Compounds of formula V may be prepared by reacting a compound of formula IX

R

4 wherein R 2

R

3

R

4 and R s are as hereinbefore defined and X is a halogen, with thiourea, or analogously, using known procedures for preparing aminothiazoles. For example as described in the Examples below or as described in European patent specification EP 117082 A. The reaction may be carried out in an organic solvent, e.g. an alcohol such as ethanol. The reaction temperature may be from room temperature to the reflux temperature of the solvent, but conveniently from about 50° C to about 70 C.

Compounds of formula V where R 2

R

3

R

4 and R S are as hereinbefore defined, may also be prepared by hydrolysing a compound of formula X

R

2

N

Rs R S C-CH 3

X

Swhere R 2

R

3

R

4 and R 5 are as hereinbefore defined, using aqueous sodium hydroxide or Shydrochloric acid solution at temperatures of SOOC to the reflux temperature of the solvent. A N co-solvent, preferably ethanol may be added to aid solubility of the starting material.

Compounds of formula VI are commercially available or may be prepared by known methods, 00 or analogously, e.g. as described in the Examples.

SCompounds of formula VII may be prepared by known methods, for example as described in R. Jain and L.A. Cohen, Tetrabedron (1996), 52, p536 3 -5 3 C Compounds of formula VIII are commercially available or may be prepared by known methods, or analogously, e.g. as described in the Examples.

Compounds of formula IX are commercially available or may be prepared by reacting a compound of formula XI

RC-O

Rs I XI

R

wherein R 2

R

3

R

4 and R5 are as hereinbefore defined, with a halogenating agent, for example bromine, or analogously, e.g. as described in the Examples.

Compounds of formula X, where R 3 is -SO 2

CH

3

R

4 is NR 8

R

9 and R 2 and R s are as hereinbefore defined, may be prepared from compounds of formula IX where R 3 is S02CH 3

R

4 is a halogen, preferably fluorine, and R 2 and R 5 are as hereinbefore defined, using known procedures for reacting aryl halides, ortho to an electron withdrawing group, with primary or secondary amines, or analogously, e.g. as hereinafter described in the Examples. The reaction may be carried out either neat or in an organic solvent, e.g. dimethylsulphoxide. The reaction temperature may be from 1000 C to 170° C but conveniently about 120 C to 140° C.

Compounds of formula X, where R 3 is -SOzCH 3 and R 2

R

4 and R s are as hereinbefore defined, may be prepared from compounds of formula X, where R 3 is -SO 2 CI and R2, R4 and

R

S are as hereinbefore defined, using the procedure known in R. W. Brown, J. Org. Chem., (1991), 56, 4974 for converting sulfonyl halides to sulfones, or analogously, e.g. as hereinafter described in the Examples. The procedure may be carried out using an alkali metal sulphite, sodium sulphite, and an alkali metal bicarbonate, e.g. sodium bicarbonate, in water at a

F

temperature from 200 C to 1000 C, but conveniently at about 750 C. The reaction with N bromoacetic acid may be carried out at temperature from 500 C to 1500 C, but conveniently at about 1000 C. An alkyl halide, e.g. iodomethane may be used in place of bromoacetic acid.

00 Compounds of formula X where R 2

R

3

R

4 and Rs are as hereinbefore defined, may be 0 prepared from compounds of formula IX, where R 2

R

3

R

4 and R3 are as hereinbefore defined, C' as described analogously for the preparation of compounds of formula V, where R 2

R

3

R

4 and R are as hereinbefore defined, but using N-acetyl thiourea instead of thiourea.

O

Compounds of formula X, where R 3 is -SOz-Cl and R 2

R

4 and R s are as hereinbefore defined, may be prepared by reacting compounds of formula X, where R 3 is -NH2 and R 2

R

4 and R 5 are as hereinbefore defined, with nitrous acid to give a diazo compound which is then reacted with sulphur dioxide in the presence of copper chloride, for example by the method described in E.

E. Gilbert, Synthesis (1969),1-10, to give the corresponding sulfonyl chlorides.

Compounds of formula X, where R 3 is -NH2 and R 2

R

4 and R S are as hereinbefore defined, may be prepared by reduction of compounds of formula X, where R 3 is -NO 2 and R 2

R

4 and

R

s are as hereinbefore defined using standard techniques known for the reduction of aromatic nitro compounds to anilines, for example catalytic hydrogenation using a transition metal catalyst, preferably palladium on carbon, in an organic solvent, e.g. ethyl acetate, under an atmosphere of hydrogen.

Compounds of formula X, where R 3 is -NO 2 and R 2

R

4 and R 5 are as hereinbefore defined are prepared by known procedures, for example as described in J. Liebscher, E. Mitzner, Synthesis, (1985), 4, 414-417.

Compounds of formula XI are commercially available or may be prepared from compounds of formula XII

H

S I

R

R

where R 3

R

4 and RI are as hereinbefore defined, using the method described in RV.

Heinzelman, Org. Synth. (1963), IV, 573, or analogously, e.g. as described in the Examples.

Compounds of formula XI where R 3 is halo, R 4 is -SO 2

CH

3 and R 2 and Rs are as hereinbefore T defined may be prepared from a compound of formula XI where R 3 is halo, R 4 is hydrogen and

R

2 and Rs are as hereinbefore defined, using standard procedures e.g. treatment with chlorosulfonic acid followed by reduction with sodium sulfite using the procedure known in R.

W. Brown, J. Org. Chem., (1991), 56, 4974 for converting sulfonyl halides to sulfones, or 00 analogously, e.g. as hereinafter described in the Examples. The reduction may be carried out 0with an alkali metal sulphite, e.g. sodium sulphite, and the alkali metal bicarbonate, e.g.

OC sodium bicarbonate in water at a temperature from 20° C to 100° C, but conveniently at about 750 C followed by alkylation with methyl iodide.

Compounds of formula XII are commercially available or may be prepared by known methods, or analogously, e.g. as described in the Examples.

Compounds of formula XII where R 3 is -SO 2

CH

3 and R 4 and R 5 are as hereinbefore defined may be prepared from compounds of formula XII where R 3 is halo and R 4 and R s are as hereinbefore defined, for example by the method described by A. Ulman and E. Urankar in J.

Org. Chem., (1989), 54, p 4691-4692, or analogously, e.g. as described in the Examples.

Compounds of formula I in free form may be converted into salt form, and vice versa, in a conventional manner. The compounds in free or salt form can be obtained in the form of hydrates or solvates containing a solvent used for crystallization. Compounds of formula I can be recovered from reaction mixtures and purified in a conventional manner. Isomers, such as enantiomers, may be obtained in a conventional manner, e.g. by fractional crystallization or asymmetric synthesis from correspondingly asymmetrically substituted, e.g. optically active, starting materials.

Compounds of formula I and their pharmaceutically acceptable salts, hereinafter referred to alternatively as agents of the invention, are useful as pharmaceuticals. In particular, they exhibit inhibition of phosphatidylinositol 3-kinase (Pi3 kinase) enzymes, especially the gamma isoform (pl10y), which are responsible for generating phosphorylated signalling products. The inhibitory properties of compounds of formula I may be demonstrated in the following test procedures: Baculovirus expressing different fragments of PI3Ky fused to GST have been previously described by Stoyanova, Bulgarelli-Leva, Kirsch, Hanck, Klinger, Wetzker, Wymann, M.P. (1997) Lipid- and protein kinase activities of G protein-coupled PI 3-kinase g: structure-activity analysis and interactions with wortmannin. Biochem. 324:489.

SResidues 38-1102 of human PI3Ky are subcloned into the BamH1 and EcoR1 sites of the _transfer vector pAcG2T (Pharmingen) to create a GST-PI3Ky lacking the first 37 residues of PI3Ky. To express the recombinant protein, Sf9 (Spodoptera frugiperda 9) insect cells are routinely maintained at densities between 3 X 10 5 and 3 X 106 cells/ml in serum containing 0 0 TNMFH medium (Sigma). Sf9 cells, at a density of 2 X 106 are infected with human GST- SPI3XyA34 baculovirus at a multiplicity of infection of 1 for 72 hours. The infected C, cells are harvested by centrifugation at 1400 g for 4 minutes at 4' C and the cell pellets are frozen at -80' C. Both Sf9 and Sf21 cells work equally well. Sf9 cells (1X109) are resuspended in 100 ml cold C) lysis buffer (50 mM Tris-HCI pH 7.5, 1% Triton X-100, 150 mM NaCI, 1 mM NaF, 2 mM DTT and protease inhibitors. Cells are incubated on ice for 30 minutes then centrifuged at 15000 g for 20 minutes at 4' C. Purification of the supernatant sample is carried out at 4' C by affinity chromatography using SEPHAROSETM agarose gel beads coupled to glutathione (from Amersham Pharmacia Biotech). A cell lysate/GST resin ratio of 50:1 is used.

The GST resin is firstly pre-rinsed to remove ethanol preservative and then equilibrated with lysis buffer. Cell lysate (supernatant) is added (usually as 50 ml lysate to 1 ml GST resin in ml tubes) and gently rotated on a mixer at 4' C for 2-3 hours. The unbound flow through sample is collected by centrifugation at 1000g for 5 minutes at 4' C using a DENLEY T M centrifuge. The 1 ml GST resin containing bound material is transferred to a 15 ml

FALCONT

M centrifuge tube for subsequent washing and elution steps. Firstly a series of 3 cycles of washings (mixing by gentle inversion) is performed with 15 ml ice cold wash Buffer A mM Tris-HCI pH 7.5, 1% Triton X-100, 2 mM DTI) interspersed with centrifugation at 1000g for 5 minutes at 4' C. A final single wash step is performed with 15 ml ice cold wash Buffer B (50mM Tris-HCi pH 7.5, 2 mM DTT) and then centrifuged at 1000g for 5 minutes at 4' C. The washed GST resin is finally eluted with 4 cycles of 1 ml ice cold elution buffer mM Tris-HCI pH 7.5, 10 mM reduced glutathione, 2 mM DTT, 150 mM NaCI, 1 mM NaF, ethylene glycol and protease inhibitors) interspersed with centrifugation at 1000g for minutes at 4' C. Samples are aliquoted and stored at -20' C.

An in vitro kinase assay was established that measures the transfer of the terminal phosphate of adenosine triphosphate to phosphatidylinositol. The kinase reaction is performed in a white 96 well microtitre plate as a Scintillation Proximity Assay. Each well contains 10 pl test compound in 5% dimethylsulphoxide and 20 ul assay mix (40 mM Tris, 200 mM NaC1, 2 mM ethyleneglycol-aminoethyl-tetraacetic acid (EGTA), 15 pg/ml phosphatidylinositol, 12.5 pM adenosine triphosphate (ATP), 25 mM MgCh 2 0.1 pCi The reaction is started by the addition of 20 pl of enzyme mix (40 mM Tris, 200 mM NaCI, 2 mM EGTA containing recombinant GST-pllOy). The plate is incubated at room temperature for 60 minutes and the Sreaction terminated by the adding 150 [l of WGA-bead stop solution (40 mM Tris, 200 mM NO NaCI, 2 mM EGTA, 1.3 mM ethylene diamine tetraacetic acid (EDTA), 2.6 pM ATP and mg of Wheat Germ Agglutinin-SPA beads (Amersham Biosciences) to each well. The plate is sealed, incubated at room temperature for 60 minutes, centrifuged at 1200 rpm and then OQ counted for 1 minute using a scintillation counter. Total activity is determined by adding 10 pl N of 5% dimethylsulphoxide (DMSO) and non-specific activity is determined by adding 10 ll O mM EDTA in place of the test compound.

Compounds of the Examples hereinbelow have ICso values below 0.5 pM in the CK aforementioned assay or demonstrate selectivity with respect to the y, a, 5 or 0 isoform as determined by a corresponding assay. For example the compounds of Examples 11, 19, 29, 66, 75, 89, 96, 98 and 116 have ICso pM values of (0.016,3.018,0.626), (0.012,0.009,0.028), (0.047,0.035,0.180), (0.213,2.059,2.616), (0.177,0.218,0.637), (0.195,0.190,-), (0.074,0.839,3.792), (0.038,0.934,2.150), (0.044,1.500,0.420) and (0.032,0.156,0.170) respectively, and compounds of Examples 121, 124, 131, 138, 145 and 150 have ICso pM values of 0.019, 0.007, 0.017, 0.018, 0.017 and 0.056 respectively.

Having regard to their inhibition of phosphatidylinositol 3-kinase enzymes, compounds of formula I in free or pharmaceutically acceptable salt form, hereinafter alternately referred to as "agents of the invention", are useful in the treatment of conditions which are mediated by the activation of the Pi3 kinase enzymes, particularly inflammatory or allergic conditions.

Treatment in accordance with the invention may be symptomatic or prophylactic.

Accordingly, agents of the invention are useful in the treatment of inflammatory or obstructive airways diseases, resulting, for example, in reduction of tissue damage, airways inflammation, bronchial hyper-reactivity, remodelling or disease progression. Inflammatory or obstructive airways diseases to which the present invention is applicable include asthma of whatever type or genesis including both intrinsic (non-allergic) asthma and extrinsic (allergic) asthma, mild asthma, moderate asthma, severe asthma, bronchitic asthma, exercise-induced asthma, occupational asthma and asthma induced following bacterial infection. Treatment of asthma is also to be understood as embracing treatment of subjects, e.g. of less than 4 or 5 years of age, exhibiting wheezing symptoms and diagnosed or diagnosable as "wheezy infants", an established patient category of major medical concern and now often identified as incipient or early-phase asthmatics. (For convenience this particular asthmatic condition is referred to as "wheezy-infant syndrome".) Prophylactic efficacy in the treatment of asthma will be evidenced by reduced frequency or Sseverity of symptomatic attack, e.g. of acute asthmatic or bronchoconstrictor attack, IND improvement in lung function or improved airways hyper-reactivity. It may further be evidenced by reduced requirement for other, symptomatic therapy, i.e. therapy for or intended O to restrict or abort symptomatic attack when it occurs, for example anti-inflammatory (e.g.

00 corticosteroid) or bronchodilatory. Prophylactic benefit in asthma may in particular be 0 apparent in subjects prone to "morning dipping". "Morning dipping" is a recognised asthmatic syndrome, common to a substantial percentage of asthmatics and characterised by asthma attack, e.g. between the hours of about 4 to 6 am, i.e. at a time normally substantially Sdistant form any previously administered symptomatic asthma therapy.

Other inflammatory or obstructive airways diseases and conditions to which the present invention is applicable include acute lung injury (ALI), adult respiratory distress syndrome (ARDS), chronic obstructive pulmonary, airways or lung disease (COPD, COAD or COLD), including chronic bronchitis or dyspnea associated therewith, emphysema, as well as exacerbation of airways hyper-reactivity consequent to other drug therapy, in particular other inhaled drug therapy. The invention is also applicable to the treatment of bronchitis of whatever type or genesis including, acute, arachidic, catarrhal, croupus, chronic or phthinoid bronchitis. Further inflammatory or obstructive airways diseases to which the present invention is applicable include pneumoconiosis (an inflammatory, commonly occupational, disease of the lungs, frequently accompanied by airways obstruction, whether chronic or acute, and occasioned by repeated inhalation of dusts) of whatever type or genesis, including, for example, aluminosis, anthracosis, asbestosis, chalicosis, cystic fibrosis, ptilosis, siderosis, silicosis, tabacosis and byssinosis.

Having regard to their anti-inflammatory activity, in particular in relation to inhibition of eosinophil activation, agents of the invention are also useful in the treatment of eosinophil related disorders, e.g. eosinophilia, in particular eosinophil related disorders of the airways involving morbid eosinophilic infiltration of pulmonary tissues) including hypereosinophilia as it effects the airways and/or lungs as well as, for example, eosinophil-related disorders of the airways consequential or concomitant to Loffler's syndrome, eosinophilic pneumonia, parasitic (in particular metazoan) infestation (including tropical eosinophilia), bronchopulmonary aspergillosis, polyarteritis nodosa (including Churg-Strauss syndrome), eosinophilic granuloma and eosinophil-related disorders affecting the airways occasioned by drug-reaction.

Agents of the invention are also useful in the treatment of inflammatory or allergic conditions Sof the skin, for example psoriasis, contact dermatitis, atopic dermatitis, alopecia areata, IN erythema multiforma, dermatitis herpetiformis, scleroderma, vitiligo, hypersensitivity angiitis, urticaria, bullous pemphigoid, lupus erythematosus, pemphisus, epidermolysis bullosa acquisita, and other inflammatory or allergic conditions of the skin.

00 O IN Agents of the invention may also be used for the treatment of other diseases or conditions, in Sparticular diseases or conditions having an inflammatory component, for example, treatment 1 of diseases and conditions of the eye such as conjunctivitis, keratoconjunctivitis sicca, and vernal conjunctivitis, diseases affecting the nose including allergic rhinitis, and inflammatory .1 disease in which autoimmune reactions are implicated or having an autoimmune component or aetiology, including autoimmune haematological disorders haemolytic anaemia, aplastic anaemia, pure red cell anaemia and idiopathic thrombocytopenia), systemic lupus erythematosus, polychondritis, sclerodoma, Wegener granulamatosis, dermatomyositis, chronic active hepatitis, myasthenia gravis, Steven-Johnson syndrome, idiopathic sprue, autoimmune inflammatory bowel disease ulcerative colitis and Crohn's disease), endocrine opthalmopathy, Grave's disease, sarcoidosis, alveolitis, chronic hypersensitivity pneumonitis, multiple sclerosis, primary billiary cirrhosis, uveitis (anterior and posterior), keratoconjunctivitis sicca and vernal keratoconjunctivitis, interstitial lung fibrosis, psoriatic arthritis and glomerulonephritis (with and without nephrotic syndrome, e.g. including idiopathic nephrotic syndrome or minal change nephropathy).

Other diseases or conditions which may be treated with agents of the invention include septic shock, rheumatoid arthritis, osteoarthritis, proliferative diseases such as cancer, atherosclerosis, allograft rejection following transplantation, stroke, obesity, restenosis, diabetes, e.g. diabetes mellitus type I (juvenile diabetes) and diabetes mellitus type II, diarrhoeal diseases, ischemia/reperfusion injuries, retinopathy, such as diabetic retinopathy or hyperbaric oxygeninduced retinopathy, and conditions characterised by elevated intraocular pressure or secretion of ocular aqueous humor, such as glaucoma.

The effectiveness of an agent of the invention in inhibiting inflammatory conditions, for example in inflammatory airways diseases, may be demonstrated in an animal model, e.g. a mouse or rat model, of airways inflammation or other inflammatory conditions, for example as described by Szarka et al, J. Immunol. Methods (1997) 202:49-57; Renzi et al, Am. Rev.

Respir. Dis. (1993) 148:932-939; Tsuyuki et al.,J. Clin. Invest. (1995) 96:2924-2931; and Cernadas et al (1999) Am. J. Respir. Cell Mol. Biol. 20:1-8.

The agents of the invention are also useful as co-therapeutic agents for use in combination with Sother drug substances such as anti-inflammatory, bronchodilatory or antihistamine drug IN substances, particularly in the treatment of obstructive or inflammatory airways diseases such as those mentioned hereinbefore, for example as potentiators of therapeutic activity of such O drugs or as a means of reducing required dosaging or potential side effects of such drugs. An 00 agent of the invention may be mixed with the other drug substance in a fixed pharmaceutical composition or it may be administered separately, before, simultaneously with or after the other drug substance. Accordingly the invention includes a combination of an agent of the invention as hereinbefore described with an anti-inflammatory, bronchodilatory, antihistamine 0or anti-tussive drug substance, said agent of the invention and said drug substance being in the same or different pharmaceutical composition.

Suitable anti-inflammatory drugs include steroids, in particular glucocorticosteroids such as budesonide, beclamethasone dipropionate, fluticasone propionate, ciclesonide or mometasone furoate, or steroids described in WO 02/88167, WO 02/12266, WO 02/100879, WO 02/00679 (especially those of Examples 3, 11, 14, 17, 19, 26, 34, 37, 39, 51, 60, 67, 72, 73, 99 and 101), WO 03/035668, WO 03/048181, WO 03/062259, WO 03/064445, WO 03/072592, non-steroidal glucocorticoid receptor agonists such as those described in WO 00/00531, WO 02/10143, WO 03/082280, WO 03/082787, WO 03/104195, WO 04/005229; LTB4 antagonists such LY293111, CGS025019C, CP-195543, SC-53228, BIIL 284, ONO 4057, SB 209247 and those described in US 5451700; LTD4 antagonists such as montelukast and zafirlukast; PDE4 inhibitors such cilomilast (Ariflo® GlaxoSmithKline), Roflumilast (Byk Gulden),V-11294A (Napp), BAY19-8004 (Bayer), SCH-351591 (Schering-Plough), Arofylline (Almirall Prodesfarma), PD189659 PD168787 (Parke-Davis), AWD-12-281 (Asta Medica), CDC-801 (Celgene), SelCID(TM) CC-10004 (Celgene), VM554/UM565 (Vernalis), T-440 (Tanabe), KW-4490 (Kyowa Hakko Kogyo), and those disclosed in WO 92/19594, WO 93/19749, WO 93/19750, WO 93/19751, WO 98/18796, WO 99/16766, WO 01/13953, WO 03/104204, WO 03/104205, WO 03/39544, WO 04/000814, WO 04/000839, WO 04/005258, WO 04/018450, WO 04/018451, WO 04/018457, WO 04/018465, WO 04/018431, WO 04/018449, WO 04/018450, WO 04/018451, WO 04/018457, WO 04/018465, WO 04/019944, WO 04/019945, WO 04/045607 and WO 04/037805; A2a agonists such as those disclosed in EP 409595A2, EP 1052264, EP 1241176, WO 94/17090, WO 96/02543, WO 96/02553, WO 98/28319, WO 99/24449, WO 99/24450, WO 99/24451, WO 99/38877, WO 99/41267, WO 99/67263, WO 99/67264, WO 99/67265, WO 99/67266, WO 00/23457, WO 00/77018, WO 00/78774, WO 01/23399, WO 01/27130, WO 01/27131, WO 01/60835, WO 01/94368, WO 02/00676, WO 02/22630, WO 02/96462, WO 031086408, WO 04/039762, WO 04/039766, WO 041045618 and WO 04/046083; A2b antagonists such as those described in WO) 02/42298; and beta-2 adrenoceptor agonists such as IND albuterol (salbutamol), metaproterenol, terbutaline, salmeterol fenoterol, procaterol, and especially, formorerol and pharmaceutically acceptable salts thereof, and compounds (in free or salt or solvate form) of formula I of WO 0075114, which document is incorporated herein by 00 reference, preferably compounds of the Examples thereof, especially a compound of formula IND 0 CH3 HO- CH

OH

and pharmaceutically acceptable salts thereof, as well as compounds (in free or salt or solvate form) of formula I of WO 04/16601, and also compounds of WO 04/033412.

Suitable bronchodilatroiy drugs include anticholinergic or antimuscarinic agents, in particular ipratropium. bromide, oxitropium bromide, tiotropium salts and CHP 4226 (Chiesi), and glycopyrrolate, but also those described in WO 01/04118, WO 02/51841, WO 02/53564, WO 03/00840, WO 03/8 7094, WO 04/05285, WO 02100652, WO 03153966, EP 424021, US 5171744, US 3714357, WO 03/33495 and WO 04/018422.

Suitable antihistamine drug substances include cecirizine hydrochloride, acetamninophen, clemastine fumarate, promethazine, loratidine, desloratidine, diphenhydramnine and fexofenadine hydrochloride, activastine, astemizole, azelastine, ebastine, epinastine, mizolastine and tefenadine as well as those disclosed in WO 03/099807, WO 04/026841 and JP 2004107299.

ather useful combinations of agents of the invention with anti-iniflammatory drugs are those with antagonists of chemokine receptors, e.g. CCR-1, CCR-2, CCR-3, CCR-4, CCR-5, CCR-6, CCR-7, CCR-8, CCR-9 and CCR1O, CXClU, CXCR2, CXCR3, CXCR4, CXCRS, particularly CCR-S antagonists such as Schering-Plough antagonists SC-35 1125, SCH-55700 and SCH--D, Takeda antagonists such as N-[[4-[[[6,7-dihydro-2-(4-methylphenyJ).SH-benzocyclohepten-8-ylcarbonyl]amino]phenyl1mehyl]erahydroN,Ndimethyl2H-pyran-4aminium chloride (TAX-770), and CCR-5 antagonists described in US 6166037 (particularly claims 18 and 19), WO 00166558 (particularly claim WO 00166S59 (particularly claim WO 04/018425 and WO 04/026873.

The agents of the invention may be administered by any appropriate route, e.g. orally, for Sexample in the form of a tablet or capsule; parenterally, for example intravenously; by IND inhalation, for example in the treatment of inflammatory or obstructive airways disease; intranasally, for example in the treatment of allergic rhinitis; topically to the skin, for example in the treatment of atopic dermatitis; or rectally, for example in the treatment of inflammatory 00 bowel disease.

The present invention also provides a pharmaceutical composition comprising a compound of I formula I in free form or in the form of a pharmaceutically acceptable salt, optionally together Swith a pharmaceutically acceptable diluent or carrier therefor. The composition may contain a co-therapeutic agent such as an anti-inflammatory, bronchodilatory or antihistamine drug as hereinbefore described. Such compositions may be prepared using conventional diluents or excipients and techniques known in the galenic art. Thus oral dosage forms may include tablets and capsules. Formulations for topical administration may take the form of creams, ointments, gels or transdermal delivery systems, e.g. patches. Compositions for inhalation may comprise aerosol or other atomizable formulations or dry powder formulations.

When the composition comprises an aerosol formulation, it preferably contains, for example, a hydro-fluoro-alkane (HFA) propellant such as HFA134a or HFA227 or a mixture of these, and may contain one or more co-solvents known in the art such as ethanol (up to 20% by weight), and/or one or more surfactants such as oleic acid or sorbitan trioleate, and/or one or more bulking agents such as lactose. When the composition comprises a dry powder formulation, it preferably contains, for example, the compound of formula I having a particle diameter up to microns, optionally together with a diluent or carrier, such as lactose, of the desired particle size distribution and a compound that helps to protect against product performance deterioration due to moisture e.g. magnesium stearate. When the composition comprises a nebulised formulation, it preferably contains, for example, the compound of formula I either dissolved, or suspended, in a vehicle containing water, a co-solvent such as ethanol or propylene glycol and a stabiliser, which may be a surfactant.

The invention includes an agent of the invention in inhalable form, e.g. in an aerosol or other atomisable composition or in inhalable particulate, e.g. micronised form, an inhalable medicament comprising an agent of the invention in inhalable form; a pharmaceutical product comprising such an agent of the invention in inhalable form in association with an inhalation device; and an inhalation device containing an agent of the invention in inhalable form.

O

(O

0 0 24 Dosages of agents of the invention employed in practising the present invention will of course vary depending, for example, on the particular condition to be treated, the effect desired and the mode of administration. In general, suitable daily dosages for oral administration are of the order of 0.1 to 10 mg/kg.

EXAMPLES

Abbreviations used are-as follows: CDI is 1,1'-carbonyldiimidazole, DCM is dichloromethane, DIPEA is diisopropylethylamine, DMF is Dimethylformamide, THF is tetrahydrofuran, HPLC is High Performance Liquid Chromatography, DMF-DMA is N,N-Dimethylformamide dimethylacetal, DMSO is dimethyl sulfoxide, HC1 is Hydrochloric acid, TFA is Trifluoroacetic acid. HOBT is Hydroxy benzotriazole, and HOAt is Hydroxy azabenzotriazole.

Preparation of intermediates The following aminothiazole intermediates of formula (A)

H

3 C

N

S-NH,

4 are shown in Table 1 below, their method of preparation being described hereinafter.

TABLE 1 Intermediate R 3

R

4 M/s

MH+

AA -SOzCH 3 F 287.11 AB -SOzCH3 CFs 337.00 AC -SO 2

CH

3 Cl 302.99 AD -SOzCH3 CN 294.02 AE -SOzCH 3 H 268.90 AF -SOzCH 3 1 335.07

N

AG F -SOzCH 3 286.99 The amines that are used to prepare the final compounds of Examples in Tables 4 5 are commercially available or are prepared by standard methods. The amines exemplified in Table 3 and used to prepare the final compounds of the Examples in Table 6 are not readily commercially available, the methods of preparation being described below.

Intermediate AA 5-(3-Fluoro-4-methanesulfonyl-phenvl)-4-methyl-thiazol-2-vlamine 00 OO I AA1) 3 -Fluoro-4-methanesulfonyl-benzaldehyde: O Methane sulfinic acid sodium salt (20.1 g, 200 mmol) is added to a stirred solution of 3,4difluorobcnzaldchydc (22.5 g, 158 mmol) in dry DMSO (200 ml) at 75* C. After 2 hours the reaction is poured onto ice-water (200 ml). The precipitate is filtered, washed with water and dissolved in chloroform (400 ml). The organic extract is washed with water (2 x 200 ml), dried over MgSO 4 filtered, and the solvent is removed to give the title compound as a white solid.

AA2) 2 -Fluoro-l-methanesulfonyl-4-(2-nitro-propenyl)-benzene: A stirred mixture of 3-fluoro-4-methanesulfonyl-benzaldehyde (Example AA1) (24 g, 0.119 mol), nitroethane (70 ml, 0.97 mol) and ammonium acetate (2.75 g, 35 mmol) is heated at reflux under argon for 24 hours. The mixture is concentrated to give an oil which is dissolved in chloroform (200 ml) and washed with water (2 x 200 ml), followed by brine (100 ml). The organic extract is dried (MgSO 4 filtered and the solvent removed to give the product as an orange oil. This was used immediately in the next step.

AA3) l-( 3 -Fluoro-4-methanesulfonyl-phenyl)-propan-2-one: Iron powder (25 g, 0.45 mol) is added to a stirred mixture of freshly prepared 2-fluoro-1methanesulfonyl-4-(2-nitro-propenyl)-benzene (Example AA2) (29 g, 0.112 mol) in THF ml). Water (110 ml) is added and the mixture is heated to 60° C. Concentrated hydrochloric acid (50 ml) is added slowly over Ih at 60-90* C. The reaction is then stirred at 1000C for hours then diluted with cold water (500 ml) and filtered through Celite

T

M filter material washing with chloroform (500 ml). he organic extract is washed with water (200 ml) followed by brine (200 ml). After drying (MgSO 4 the mixture is absorbed on silica and purified by chromatography, eluting with hexane ethyl acetate to give the titled compound.

AA4) 5-(3-Fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiaol-2-ylamine: 1-( 3 -Fluoro-4-methanesulfonyl-phenyl)-propan-2-one (AA3) (1.0 g, 4.34 mmol) is dissolved in dioxane (35 ml) and the solution is cooled to 10* C at which point the mixture is semi frozen.

Bromine (0.201 ml, 3.6 mmol, 0.8 eq.) is added slowly and the mixture is stirred for an additional 15 min in a semi frozen state. The mixture is then allowed to warm to room 26 temperature and the solvent is removed to give a brown oil containing starting material and Ibromo.1-(3-fluoro)-4-methanesulfony-phenyl)-propan-2-one. This material is dissolved in ethanol (30 nil) and thiourea (0.236 g, 3.1 mmol) is added in one portion. The mixture is stirred at 60 C for 30 minutes then allowed to cool whereupon the product crystallised.

Filtration affords the hydrobromide salt of the product as a white solid. The free base is 00prprdbdisligtehdormdsatidiueauoshdohoiacdadadn prprdbIisligtehdrboieslNndltDqeushdohoi cdadadn sodium hydroxide solution until alkaline. The title compound precipitates as the free base.

Intermediate AB 5-(4-Methanesulfony-3-trifluoromethy-phenyl)-4-methyl-thiazol-2-ylamine AMi) N-15-(4-Methanesulfonyl-3-trifluoromethyl-phenyl)-4-methyl-thiazol-2-yi-acetamid:- The titled compound is made via an analogous method to 5-(3-Fluoro-4-methanesulfonylphenyl)-4-methyl-thiazol-2-ylamine (Example AA) by replacing 3,4-difluorobenzaldehyde (step AA1) with 4-.chloro-3-trifluoromethyl-benzaldehyde, and thiourea (step AA4) with Nacetylthiourea.

AIB2) 5-(4-.Methariesulfonyl-3-trifluoromethyl-phenyl)-4-methyl-thiazol-2-ylamine: N-[S-(4-Methanesulfonyl-3-trifluoromethyl-phenyl)-4-methyl-thiazol-2-yl]-acetamide (ABi) (0.115 g, 0.3 mmol) is suspended in ethanol (5 ml). Hydrochloric acid (1.58 ml, 6N HCI) is added and the reaction mixture is heated at reflux for two hours. The solvent is removed in vacuo and the crude residue is suspended in water and sodium hydroxide solution is added until the p1- is adjusted to p1-1 13-14. The yellow precipitate is stirred at room temperature for minutes and then filtered, washed with water and dried in vacuo to yield the titled compound.

Intermediate AC 5-(3-Chloro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-ylamine This material is prepared by the procedure outlined in Experiment AA, replacing 3,4.

difluorohenzaldehyde in the first step (AA1) with 3,4-dichloro-benzaldehyde.

Intermediate AD 5-(2-Amino-4-methyl-thiazol-5-ylh-2-methanesulfonyl-benzonitrile This material is prepared by the procedure outlined in Experiment AA, replacing 3,4difluorobenzaldehyde in the first step (AAI) with Intermediate AE 1 S-(4-Methanesulfonyl-phenvl)-4-methyl-thiazol-2-ylamine AE1) N [5-(4--Amino-phenyl)-4-methyl-thiazol-2-yl]-acetamide: N-[4-Methyl-S-(4-nitro-phenyl)-thiazol-2-yl]-acetamide Liebscher, E. Mitzner, Synthesis, 00 1985, p 414) (10.0 g, 3.6 mmol) is dissolved in ethyl acetate TIF 600 ml) and O stirred at room temperature under an atmosphere of argon. The solution is then treated with 10% palladium on carbon (10 The reaction mixture is purged three times with nitrogen and I> placed under an atmosphere of hydrogen overnight. The mixture is then filtered through SCelite T M filter material and the catalyst is washed with tetrahydrofuran (600 ml). The solvent is removed in vacuo to leave N-[5-(4-amino-phenyl)-4-methyl-thiazol-2-yl]-acetamide as an offwhite solid.

AE2) 4-(2-Acetylamino-4-methyl-thiazol-5-yl)-benzenesulfonyl chloride: N-[5-(4-Amino-phenyl)-4-methyl-thiazol-2-yl]-acetamide (AE1) (7.9 g, 31.9 mmol) in suspension in glacial acetic acid (250 ml) is treated with a 32 aqueous HCI solution (40 ml).

The resulting solution is then cooled approximately to 10* C and treated dropwise with a solution of sodium nitrite (2.2 g, 31.9 mmol) in water (2 ml). After 10 minutes the reaction mixture is added to a stirred solution of SO/AcOH/CuCl 2 /HzO (200 ml) (the preparation of the reagent is described below). The reaction mixture is allowed to warm to room temperature.

After stirring overnight the reaction mixture is poured into water (1000 ml) and extracted with ethyl acetate (3 x 300 ml). The combined organic layers are washed with water (2 x 250 ml) followed by brine (200 ml) and dried over MgSO4. After filtration the solvent is removed in vacuo to give the titled compound.

Preparation of the reagent SO 2 /AcOH/CuCl2/HIO: According to the reported procedure E. Gilbert, Synthesis 1969, 1-10, p6), glacial acetic acid (100 ml) vigorously stirred at room temperature is treated by bubbling SO2 gas. Once a saturated solution is achieved (approximately 10 g per 100 ml), the solution is treated with copper (II) chloride (4 g) in water (5 ml). The resulting mixture is allowed to settle to give a green solution.

AE3) 5-(4-Methanesulfonyl-phenyl)-4-methyl-thiazol-2-ylamine: 4-(2-Acetylamino-4-methylthiazol-5-yl)-benzenesulfonyl chloride (AE2) (0.5 g, 1.5 mmol) in dioxane (2 ml) is added dropwise to a stirred solution of sodium sulfite (0.378 g, 3.0 mmol) and sodium hydrogen carbonate (0.252 g, 3.0 mmol) in water at 75" C. After 1 hour at 75° C, ,fl bromoaceric acid (0.417 g, 3.0 mmol) is added and heating continued for 1 hour at 1000 C.

Sodium hydroxide (0.24 g, 6.0 mmol) in water (0.25 ml) is then added and the mixture is IND heated with stirring at 900 C for 16 hours. The reaction mixture is allowed to cool, diluted with water (100 ml) and extracted with dichloromethane (3 x 75*ml). The combined organic extracts are washed with brine (75 ml), dried (MgSO4), filtered, and the Solvent removed to give the title compound.

Intermediate AF 5-(3-Imidazol- I -yl-4-meth anesulIfonyl-phenyl) -4-methy -thiazol -Z-Xla mine (71AFI) N-[S-(3-fluoro-4-methanesulfonyl-phenyl)A4-methyl-thiazol-2-y1]acetamide: This material is prepared from 1-(3-fluoro-4-methanesulfonyl-phenyl)-propan-2-one (AA3) following the procedure outlined in step (AA4), replacing thiourea with N-acetylthiourea. The titled compound crystallises from the reaction mixture.

A172) 3 -Imidazol-1.yl-4-methanesulfonyA-phenyl).4-methylbrhiazol-2yl]-aceamide: A stirred mixture of N-[5-(3-fluoro- 4-methanesulfonyl-phenyl)-4-methyl-thiazo[-2-yly acetamide (AFI) (5.0 g, 15 mmol), imidazole (2.07 g, 30 mmol) and caesiumn carbonate (9.93 g, 3 0 mmol) in dry NMP (30 ml) is heated under argon at 135 0 C for 18 hours. The reaction mixture is then poured onto water (50 ml) whereupon the titled product precipitates and is recrystallised from ethanol water.

AF3) 5-(3-Imidazol-1 -yl-4-methanesulfony1-phenyl)-4-methyl-rhiazol.2.ylamine: 3 -Imidazol-1.yl4-methanesulfonylphenyl)4methylrthiazol.2-yll-acetamide (AF2) g, 13 rnol) is dissolved in 7M HCI (50 ml) and the solution is heated at 100 0 C for 3h. When cool the solution is brought to pH- 8 by addition of aqueous NaOH whereupon the titled product precipitates. This is washed with ethanol followed by EtOAc, Et 2 O0 and dried.

Intermediate AG 4 -Fluoro-3-merhanesulfonyl-henyl)4-mehvl-thiazol-2ylamine AGi) 2 -Fluoro-S-(2-oxo-propyl)-benzenesulfonyl chloride: To a stirred flask of chlorosuiphonic acid (344 g, 2.96 mol) cooled to -10* C is added dropwise 1-(4-fluoro-phenyl)-propan-2-one (10 g, 65 mmol). The reaction mixture is left stirring at room temperature overnight and then poured carefully onto ice. The resultant solution is 29 extracted with ethyl acetate and the organic extracts combined, dried (MgSO4) and concentrated in vacua to yield the titled compound.

AG2) 2-Fluoro-S-(2-oxo-propyl)-benzenesulfinic acid sodium salt: To a stirred solution of 2-fluoro-S-(2-oxo-propyl)-benzenesulfony chloride (AGi) (16.25 g, 00 86.36 mmol) in dioxanc (200 ml) is added dropwise a solution of sodium sulfite (16.38 g, 130 IND mmol) and sodium hydrogen carbonate (10.92 g, 130 mnmol) in water (200 ml). The reaction mixture is heated to 70 C for 20 minutes and then allowed to cool to room temperature. The solvent is removed in vacua and the resultant residue is used crude in the next step.

r~l AG3) 1 -(4-Fluoro-3-methanesulfonyl-phenyl)-propan-2-one: A solution comprising 2-fluoro-5-(2-oxo-propyl)-benzenesulfinic acid sodium salt (AG2) (15.43 g, 64.83 mmol) in DMF (300 ml) is treated with methyl iodide (18.45 g, 130 mmol).

The reaction mixtre is stirred at room temperature for 30 minutes. Purification is carried out by chromatography on silica (eluting with 4:1 hexane-ethyl acetate) to afford the titled compound.

AG4) 5-(4-Fluoro-3-methanesulfonyl-phenyl)-4-methyl-thiazol-2-ylamine: The titled compound is prepared via an analogous method to 5-(3-fluoro-4-methanesulfonylphenyl)-4-methyl-thiazol-2-ylamine (Intermediate AA) by replacing 1-(3-fluoro-4methanesulfonyl-phenyl)-propan-2-one (AA3) in step AA4 with 1-(4-fluoro-3-methanesulfonylphenyl)-propan-2-one (AG3).

The following imidazole-urea intermediates of formula (B)

H

3 C

N

S ,IC-N\)r B are shown in the Table 2 below, the method of preparation being described hereinafter.

TABLE 2 Intermediate R3' R4 Starting Method materia BA -SO 2

CH

3 F AA Ba BB -SO 2

CH

3

CF

3 AB Ba BC "SQ2CH 3 C1 AC Ba Intermediate RR4 Starting Method material IDBD

-SO

2

CH

3 CN AD *Bb BE -S02CH 3 H AE Ba BF -S0 2 CH3 IAF Bb

N

00BG F -SO2ClHb AG Ba Method (Ba) A suspension of the aminothiazole (17.5 mmol) and 1,1'-carbonyldiimidazole (4.

2 6g, 26.3 mmol, 1.5 equivalents) in CF1L 2 C1 2 (100 ml) is heated at 40 0 C reflux under argon until no starting material remains (30 min 5 hours) as determined by HPLC and NMR. When cool the solid precipitate is removed by filtration. This solid consists of the imidazole-urea intermediate together with variable amounts of the corresponding isocyanate and in-ildazole which result from reversible thermal elimination of imidazole under the reaction conditions.

This solid is used in the subsequent steps since the imidazole-urea intermediate and isocyanate intermediate are equally suitable as precursors to ureas.

The following intermediates are prepared by this method, namely: Irnidazole-1-carboxylic acid [5-(3-fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yll-amide Imidazole-1carboxylic acid [5(-ehnsloy--rfurmtylpey)4mty-hao--l-md Imidazole-1 -carboxylic acid [5-(3-chloro-4-methanesulfonyl-phenyl)-4-methyl-thiazo[- 2 ylJ-amide Irnlidazole-l-carboxylic acid [S.(4-methanesulfonyl-phenyl)A-methyl-thiazob2yl]-amide (BE) and Imidazole-l-carbox-ylic acid [5-(4-fluoro-3-methanesulfonyl-phenyl)-4methyl-thiazol-2-y1-arnide

(BG).

Method (Ebl Triethylamine or sodium hydride (1.25 equivalents) is added to a stirred suspension of the aminothiazole (7.5 mmol) and carbonyldiimidazole (1.3 g, 8.2 mmol, 1.1 equivalents) in dry

CH

2

CI

2 (40 ml) containing a few drops of DMF to aid solubility. The reaction is heated at reflux under argon until no starting material remains (18 h) as determined by HPLC and NMR. When cool the solid precipitate is removed by filtration and washed with diethyl ether.

This solid consists of the carbonyl diimidazole intermediate together with variable amounts of the corresponding isocyanate and imidazole which result from thermal elimination of irnidazole under the reaction conditions. This solid is used in the subsequent steps since the CDI intermediate and isocyanate intermediate are equally suitable as precursors to ureas.

The following intermediates were prepared by this method: Iniidazole-1 -carboxYlic acid cyano-4-methanestilfonyl-phenyl)-4-methyl-thiazol-2-ylI-amide (BD) and Imidazole-1-carboxylic acid [S-(3-imidazol-1-yl-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]-araiide The following amine intermediates of formula (C)

H

2 N\ Hot Where Hot.

R

CA CB NCC N

R

CD

N-

R

CE

N=N

CJ

N2 N'

R

CH

R

are shown in the Table 3 below, the method of preparation being described hereinafter.

TABLE 3 Intermediate Het R CAl CA -C-H2CH3 CA2 CA -CH 3 CA3 GA Gel CB -GH 3 CB2 GB -CH 2

CH

3 CG1 cc -CH3 C cc -CH2CH 3 MG cc -CH2GH2GH 3 CG4 cc -CH(CH- 3 )2 MC cc -Gyclopropyl GG6 cc -C(CH 3 )3 GG7 CC -CH 2

N(CH

3 )2 CD1 GD -CH 3 GEl CE -CH 3 CE2 CE -CH 2 CH3 GE3 CE -CH 2

CH

2

CH

3 CE4 CE -CH(CH3)2 CES CE -Gyclopropyl CE6 GE -Cyclobutyl GE7 CE -C(CH 5 3 CRl CF C2H CG1 CG '-CH 2

CH

3 CHIi CHi -C(CH 3 3 CH2 GH- -CH(CH 3 )2 C1l CI -H C12 CI -CH 2

CH

3 cji CT-CH 2

CH

3 Intermediate CA1 S2-(5-Ethyl-oxazol-2-vl)-ethvlamine Step 1: 2 -(2-Hydroxy-butylcarbamoyl)-ethyl]-carbamic acid benzyl ester: A mixture comprising Z-Beta-Ala-OH (9.0 g, 40.3 mmol), EDCI.HC1 (10.0 g, 52.4 mmol), 0 hyrdoxybenzotriazole (5.45 g, 40.3 mmol), triethylamine (7.3 ml, 52.4 mmol) in DCM (150 NO ml) is stirred at 0OC for 30 minutes. 1-Amino-2-butanol (4.2 ml, 44.3 mmol) is added in one portion and stirring is continued for 1 hour. The reaction mixture is diluted with water (150 ml) and extracted with dichloromethane (2 x 150 ml). The organic layers are combined, dried O over MgSO4, filtered and concentrated in vacuo to yield a crude white solid. The product is C1 purified by chromatography on silica eluting with ethanol-ethyl acetate (1:10) to give the titled compound.

Step 2 -Oxo-butylcarbamoyl)-ethyl]-carbamic acid benzyl ester: To a stirred solution of oxalyl chloride (2 M in DCM) (13.35 ml, 26.5 mmol) in dry DCM at 78*C is added dropwise DMSO (2.5 ml, 35.4 mmol). After stirring for 15 minutes, the reaction mixture is treated with a solution of 2 -(2-hydroxy-butylcarbamoyl)-ethyl]-carbamic acid benzyl ester (step 1) (6.5 g, 22.1 mol) in dry DCM (40 ml). Triethylamine (13 ml) is added after 1 hour and after stirring at -78*C for 90 minutes, the reaction mixture is allowed to warm to room temperature. The reaction is diluted with DCM (100 ml) and washed with HCI (1M, 200 ml), saturated sodium bicarbonate solution (200 ml), water (200 ml) and brine (200 ml).

The organic portion is dried over MgSO 4 filtered and concentrated in vacuo to yield the titled compound as a white solid.

Stp: 2 -(5-Ethyl-oxazol-2-yl)-ethyl]-carbamic acid benzyl ester To a stirred suspension of polymer supported triphenylphosphene (19.6 g, 58.9 mmol) in DCM (250 ml) is added iodine (14.95 g, 58.9 mmol). After stirring at room temperature for minutes, the mixture is treated with triethylamine (16.4 ml, 117.5 mmol) followed by a solution of 2 2 -oxo-butylcarbamoyl)-ethyl]-arbamic acid benzyl ester (step 2) (6.88 g, 23.5 mmol) in DCM (50 ml). The reaction mixture is stirred overnight and then filtered through Celite

T

m filter material, washed through with DCM (500 ml) and the solvent removed in vacuo to yield the titled compound as a brown solid.

Lep 4: 2 -(5-Ethyl-oxazol-2-yl)-ethylamine: Ammonium formate (0.316 g, 5 mmol) is added to a solution of [2-(5-ethyl-oxazol-2-yl)-ethyl]carbamic acid benzyl ester (step 3) (1.66mmol) in methanol (15 ml) and 10%Pd on carbon 33 (12.5 mg) is added under an inert atmosphere. The Mixture is stirred at ambient temrperature for 2 hours. The catalyst is removed by filtration and the filtrate is evaporated. The residue is IND diluted with dichioromethane, filtered to remove undissolved solid and the solvent is removed.

The residue is dissolved in DCM and treated with 1M aqueous sodium hydroxide solution (S ml). The organic extract is separated, dried (MgSO 4 filtered and the solvent is removed.

00 Crystallisation from ethyl acetate dichioromethane affords the titled compound.

intermediate CA2 2-(5-Methyl-oxazol-2-yl)-ethylamine This compound was made by an analogous procedure to 2-(S-ethyl-oxazol-2-yl)-ethylamine (Ni (CAl) by replacing 1-amino-2-butanol (CAl, step 1) with 1-amino-propan-2-ol.

Intermediate CA3 2-(5-tert-Butyl-oxazol-2-yl)-ethylaniine 51cpj: I -Azido-3,3-dimethyl-butan.2-ol: Sodium azide (10 g, 154 mmol) and ammonium chloride (3.7 g, 070 mmol) are added to a solution of 3,3-dimethyl-1,2-epoxybutane (4.26 ml, 35 mmol) in methanol :water 315 ml), and the mixture is heated at reflux for 7 hours. The reaction is poured into ice/water (400 ml) and extracted with dichloromethane (3 x 200 ml). The combined organics are dried (MgSO4), filtered and evaporated to yield a clear oil (4.7 g, 94%) Step 2: 1-Amino-3,3-dimethyl-butan-2-ol: 1 -Azido-3,3-dimethyl-butan-2-ol (4.7 g, 3 3 mmol) in ethanol (200 ml) is stirred under hydrogenated for 36 h in the presence of 10% palladium on carbon. The catalyst is removed by filtration and the filtrate is evaporated to give the titled compound as a colourless crystalline solid 2 .7g, The final compound (CA3) is then prepared by an analogous procedure to 2-(S-ethyl-oxazol-2-yl)-ethylainine (CAl) by replacing 1-amino-2-butanol (CAl, step 1) with 1-an-ino-3,3-dimethyl-butan-2-ol.

Intermediates CB1 CB2 These compounds, namely 2 4 -methyl-oxazol-2-yl)-ethylamine (CB1) and 2 -(4-ethyl-oxazol-2yl)-ethylamlne (CB2) are prepared by an identical procedure to 2-(S-Ethyl-oxazol-2-yl)ethylamine (CAl) by replacing I-amino-2-butanol (CAI, step 1) in this procedure with 2amnino-1-butanol (for CR1) and 2 -arriino-l-propanol (for CR2) respectively.

34 ,fl Intermediates CC1-CC7 These compounds, namely, 2-(3-Methy[-[1,2,4]oxadiazol-S-yl)-ethylamine (CCI), 2-(3-Erhyl- [1 ,2,4]oxadiazol-S-yl)-ethylamine (CC2), 2-(3 -Propyl-[1,2,4] oxadiazol-5-yl)-ethyla mine (MC), 2-(3-Isopropyl-[ 1,2,4]oxadiazol-5-yl)-ethylamine (GC4), 2-(3-Cyclopropyl-[1 ,2,4]oxadiazol-SyI)-ethylamine (CCS), 2-(3-tert-Butyl-[1 ,2,4]oxadiazol-S-yl)-ethylan-ine (CCG) and 2-(3- OC) Dimethylaminomcthyl-[1,2,4)oxadiazol-S-yl)-ethylamine (CG7) are prepared by an identical procedure starting from the appropriate nitrile. This is exemplified by the preparation of intermediate CC3. Preparation of the nitrile used for intermediate CG7 is described in C. S.

1-ollander; R. A. Yoncoskie 8c P. L. deBennevillej Org. Chem., (1958) 23, 112-215.

CI Intermediate CC3 2-(3-Propyl-[ I.2.41oxadiazol-5-yfllethylamine Sp~j: N-I-ydroxy-butyramidine: Ethanol (80 ml) followed by hydroxylamine hydrochloride (4.0 g, 57 mmol) is added to a solution Of K.

2 C0 3 (7.9 g, 57 mmol) in water (25 ml). Butyronitrile (5.0 ml, 57 mmol) is then added and the mixture is heated at reflux for 18 h. After cooling, the solvent is removed in vacuo and ethanol is added to dissolve the product. The solution is separated from any undissolved solid and the solvent is removed to leave the titled compound as a yellow oil.

5=p.2: [2-(3-Propyl-[1,2,4]oxadiazol-S-yl)-ethyl]-carbamiic acid tert-butyl ester: N-Hydroxy-butyramidine (0.425 g, 4.16 mmol) in DMF (2 ml) is added to a stirred suspension of sodium hydride 183 g of a 60% dispersion in oil, 4.58 mmo!) in the presence of molecular sieves (0.4 The reaction flask is then immersed in a pre-heated oil bath at 500C and stirring continued for 5 min. 3-tert-Butoxycarbonylamrino-propionic acid ethyl ester (0.904 g, 4.16 mmol) in DMF (2 ml) is added over 5 min followed by more DMF (2 ml). After 3 h at SOOC the mixture is cooled to OOC and water (3 ml) is added. The mixture is allowed to warm to room temperature then filtered through CeliteTM' filter material, washing with ethyl acetate, and the solvent is removed. Purification by chromatography, eluting with DCM:MeOH- (95:5 increasing to 85:15) affords the titled compound.

2-(3-Propyl-[1,2,4]oxadiazol-5-yl)-ethylamine: TFA (6 mld) is added to a stirred solution of [2-(3-propyl-[1,2,4]oxadiazol-S-yl)-ethyl]-carbamic acid tert-butyl ester (0.67 g, 2.62 mmol) in DCM (10 ml). After 1 hor the solvents are removed to afford the titled compound.

~fl Intermediate CD1 2-(3-Methyl-f 1l.2.41thiadiazol-5-yfl-ethylamine Step 1: [2-(l-Dimerhylamino-ethylidenethiocarbamoyl)-echyl]-carbamic acid tert-buryl ester: Dimerhylacetamide dimethylacetal (1.56 g, 11.7 mmnol) is added to a stirred solution of (2- 00 thiocarbamoyl-ethyl)-carbamic acid tert-butyl ester (1.0 g, 4.9 mmol) in DCM (50 ml). The IND reaction is stirred for 24 hours at room temperature followed by removal of the solvent. The residue is purified by chromatography on silica, eluting with ethyl acetate diethyl ether (1:1) to give the titled compound as a yellow oil.

Sieo 2: 2-(3-Methyl-[1,2,4]rhi~diazol-S-yl)-ethylamine: Hydroxylamine-O-sulfonic acid (0.68 g, 6.0 mmol) in methanol (5 ml) is added to a stirred solution of [2-(l1-Dimethylamino-ethylidenethiocarbamoyl)-ethylJ-carbamic acid tert-butyl ester (1.43 g, 5.0 mmol) and pyridine (0.83 ml, 10 nimol) in ethanol (25 ml). After stirring the reaction for 18 hours at room temperature the solvent is removed in vacuc and the residue is partitioned between water and DCM. The organic extract is separated, dried (MgSO 4 and the solvent is removed to give a colourless oil which is dissolved in ethanol (20 ml). Aqueous H1-C (2M, 10 ml) is added and the mixture is heated at reflux for 1 hour. After cooling to room temperature the solvent is removed. The product is dissolved in aqueous NaHCO 3 and the free base is extracted with ethyl acetate. The organic extract is dried (MgSO 4 and the solvent removed to give the titled compound.

Intermediate CEI 2-(S-Methyl-f 1.3 .4loxadiazol-2-yl)-ethylamine Step 1: [3-(N'-Acetyl-hydrazino)-3-oxo-*propyl]-carbamic acid tert-butyl ester: EDCI.HC1 (3.05 g, 15.95 mmnol), HOBt (1.66 g, 12.27 nimol) and triethylaniine (2.22 ml, 15.95 nimol) are added to a stirred solution of BOC-f3-Ala-OH (2.32 g, 12.27 mmol) in DCM nml). After stirring for 30 min at 0O'C acetic acid hydrazide (1.0 g, 13.5 mmol) is added in one portion. The reaction mixture is stirred at 0 0 G for 1 hour. The reaction is diluted with water and extracted several times with DCM. The organic extract is dried (MgSO4) and the solvent is removed to give an oil. Purification by chromatography on silica, eluting with EtOAc increasing to EtOAc EtOH 10: 1, affords the tided compound.

Step 2: [2-(5-Methyl-[1 ,3 ,4]oxadiazol-2-yl)-ethyl]-carbamjic acid tert-butyl ester: 36 Iodine (0.261 g, 1.028 rnmol) is added to a stirred solution of polymer supported triphenyiphosphine (3 mmoLlg, 0.34 g, 1.03 mmol) in DCM (10 ml). After stirring for 10 min IND at room temperature triethylamine (0.287 ml, 2.06 mmol) and [3-(N'-acetyl-hydrazino)- 3- oxopropyl]-carbamic acid tert-butyl ester (0.126 g, 0.514 mmol) are added. The reaction is stirred at room temperature for 18 h then filtered through a Celitem filter material plug, washing with 00 DCM. The solvent is removed and the residue is purified by chromatography on silica, eluting IND with EtOAc, to afford the titled compound.

201 2-(S-Methyl-[1 ,3,4]oxadiazol-2-yl)-ethylamine: TEA (1 ml) is added to a stirred solution of [2-(S-methyl-[1,3,4]oxadiazol-2-yl)-ethyl].

carbamic acid tert-buryl ester (0.077 g, 0.34 mmol) in DCM (5 ml). After 1 hour at room temperature the solvents are removed. The residue is dissolved in DCM and washed with 4M aqueous NaOH solution. The organic extract is separated, dried (MgSO4) and the solvent removed to give the titled compound.

Intermediates CE2-CE7 These compounds, namely 2-(S-Etbyl-[1,3,4]oxadiazoi-2.yl)-ethylaniine (CE2), 2-(S-Propyl- [1,3,4]oxadiazol-2-yl)-ethylamine (CE3), 2-(5-Isopropyl-[1 ,3,4]oxadiazol-2-yl)-ethylamine (CE4), 2 -(5-Cyclopropyl-[1,3,4]oxadiazol-2-y)-ethylamine (CES), 2-(5-Cyclobutyl-[1,3,4] oxadiazol-2-yl)-ethylamine (CE6) and 2-(5-tert-Butyl-[1 ,3,4]oxadiazol-2-yI)-ethylamine (CE7), are prepared by an analogous procedure to intermediate CEl using the appropriate hydrazide in step 1.

Intermediate CM1 CG1L 1-Ethyl.1H-imidazol-4-yl)-ethylamine 2-(3-Ethyl-3--dazol-4-yl 1-ethylamine Step 1: 2-[2-(1H-Imidazol-4-yl)-ethyl-isoindole-1,3-dione: N-Carbethoxy phthalimide (2.45 g, 11 mmol) is added over 10 minutes to a vigorously stirred solution of histamine (1.13 g, 1.0 mmol) and Na 2

CO

3 (1.19 g, 1.1 mmol) in water (22 mal).

After lh the white suspension is removed by filtration, washed with ethanol and dried to afford the titled compound.

Step 2: 1-Ethyl-l-I-imidazol-4.yl)-ethyl]-isoindole-1,3-dione 2-12-(3-ethyl-3Himidazol-4-yl)-ethyl)-isoindole 1 ,3-dione.

A suspension of 2 2 -(1H-imidazol-4-yl).ethyl]-isoindole-1,3-dione (5.0 g, 20.7 mmol) in DMF (41.5 ml) is added slowly to a stirred suspension of NaH (0.83 g of a 60% dispersion in 37 mineral oil, 21 mmol) at OOC. The mixture is stirred at QOC for 15 min followed by warming to room temperature over 45 minutes then cooling back down to 0 0 C. A solution of bromoethane 54 ml, 20.7 rnrol) in DMF (10 mld) is a dded dropwise. The reaction is stirred at 5 0 C for 30 min followed by room temperature for 24 hours. The solvent is removed and the residue is purified by chromatography on silica eluting with DOM and MeOH (1 increasing to to give a mixture of 2 2 -(l-ethyJ.1H-imidazoI-4yl)ethyl-isoindolel1 3 -dion n h 00an th INOregioisomer, 2-2(-tyl3-mdz -I -ry]-sidl-,3-dione.

2-(1 1-EthylA -Imiao--l-ryan &2(-ty3H-im idazol-4-yl)-ethylamine H-ydrazine hydrate (0.18 ml, 3.71 mmol) is added to a stirred 70:30 mixture of 2 -[2-(1-ethylisoindole-1,3-dione (1.0 g, 3.71 mmol) suspended in dry EtOH (7.4 mnl) and the mixture is heated at 95 0 C for 90 min. After cooling to room temperature the mixture is filtered and the solvent is removed from the filtrate to afford a mixture of the titled compounds as a yellow oil.

Intermediates CHI CR2 These compounds, namely 2 2 -tert-Butyl3Himidazo1Ayl)-ethylamine (CHI) and 2-(2.

Ispoy-Hiiaol4y)ehlmn (CH2) are prepared according to the protocols described in R. Jain, L.A. Cohen, N.A. EI-Kadi and M.M. King, Tetrahedron (1997), 53, pages 2365-2370.

Intermediate C11 H-Tetrazol- Syl)-et hlmine This material is prepared by the protocols outlined in N. A. Delaney, G. C. Rovnyak and M.

Loots, European patent -specification EP 449523.

Intermediate C12 S= 1:[-1-ty-I-erzo--i-ty]crai acid tert-buryl ester: A solution of [2(HTtao--l-tylcrari acid tert-butyl ester (EP 449523) (1.0 g, 4.69 mmol) in dry THF (20 ml) is treated with a 60 dispersion of sodium hydride in mineral.

oil (0.19 g, 4.69 mmol) and stirred at ambient temperature for 10 minutes. Ethyliodide (0.375 mrl, 4.69 mmol) is added and the reaction mixture is heated at reflux for 7 hours, then diluted with ethylacetate and filtered. The filtrate is evaporated and the residue purified b y flash silica chromatography (elution 3:2 hexane/ethylacetate) to afford 2 -(2-EthyJ-2H..retra2Ol-5-y) ethyl]-carbamic acid tert-butyl ester, which elutes first, and the titled compound, [2-Cl-Ethyl- 1H-tetrazol-S-yl)-ethyl]-carbamidc acid tert-butyl ester, which elutes next.

_Sgp2: 2-(1 -Ethyl-i H-tetrazol-S-yl)-ethylamine: (1 -Ethyl- 1H-terrazol-5-yl)-ethyl]-carbamic acid tert-butyl ester (0.33 g, 1.36 mmol) is dissolved in dichloromethane (3 ml) and treated with trifluoroacetic acid (1 ml) and stirred at ambient temperature for 3 hours. The solvent is removed to afford the titled compound as the TFA salt.

Intermediate CJl 2-(1 -Ethyl- I H-tetrazol-5-yl 1-ethylami ne [2-(2-Ethyl-2H-tetrazol-5-yl)-ethylJ-carbamidc acid tert-butyl ester, obtained as a second product in synthesis of [2-(l-Ethyl-1H-tetrazol-S-yl)-ethyl]-carbamic acid tert-butyl ester (Intermediate C12, step 1) is treated with TFA as described for 2-(1-Ethyl-1H-tetrazol-5-yl)ethylamine (C12, step 2) to afford the tided compound.

The following additional amine intermediates of formula (C) H2 11\

C

Hot Where Het. N

R

CE CF

/N-

R

C1

N=

Ci

~N=N

YA

N

I] R

N

CK CL CM are shown in the Table 3a below, the method of preparation being described hereinafter.

TABLE 3a Intermediate Het R CE8 CE H

OH

CH

3 CF2 CF Intermediate Het

R

CF3 CF

F

F

CF4

CFH

___CH

3 CFS CF

CH

3 CF6 CF C13 CI

O

CJ2

CJ

CJ3

CJ

CJ4 CJ

OH

H

CKl CK

CH

3 CK2 CK CMi CL

H

CMI CM

H

CM2 CM

CH

3 Intermediate CES 2454-2-Amino-ethyl-)41 3 A~oxadiazoI-2-yll-2-.methyl-propan-.1oI This material is prepared by an analogous procedure to intermediate GEl using the appropriate hydrazide in step 1.

Intermediate CF2-CF6 These compounds, namely 2-[l 2 -Fluoro-ethyI)-IH-imidazo-4-yl]yethylamine (CF2), 2-[l- (3 3 3 -Trifluoro-propyl)~-H-imidazola4-yl]yethylamine (GF3), 4 4 2 -Amino-ethyl)-imidazol-i..

yl]-butan-2-ol (CF4), (S)-3-[4-(2-Amino-erhy)-irrcJazol-l-yl]-2-methyl-propan-l-oI (CFS) and 2 2 -Chloro-erhyl)-1H-imnidazol-4yl].ethylamine, are prepared by an analogous procedure to intermediate CHl using the appropriate halo adduct in step 2.

Interediate C3 3-r5-(2-Amino-ethyl l-tetrazol-1 -vll-nronan- -cI IND This material is prepared by an analogous procedure to intermediate C12 by replacing the ethyliodide in step I with 3-bromo-1-propanol.

00 Intermediate CJ2 Z-[2.(2-Fluoro-ethyl) 2H-tetrazol-S.Yl1-ethylaminl To a solution comprising [2-(1H-Tetrazol-S-yl)-ethyl]-carbaifLic acid tert-butyl ester (EP 449523) (0.125 g, 0.586 mmol) in D10 (5 ml) is added caesium carbonate (0.23 g, 0.703 minol) followed by 1-bromo-2-fluoroethane (0.174 ml, 2.344 mmol) and the reaction mixture is left to stir at room temperature for 3 days. The solvent is removed in vacua and the crude residue is purified by flash silica chromatography (elution. 3:2 increasing to 1:4 hexane/ ethyl acetate) to afford the titled compound and 12- (2-Fluoro-ethyl)-1H-tetrazol-S -yl] -ethyl)carbamic acid tert-butyl ester.

StR2 2-[2.(2-Fluoro-ethyl)-2H-terazol-5-yl]-ethylamine: A solution of {2-[2-(2-Fluoro-ethyl)-2H-tetrazol-S-y]-ethyl-carbamfic acid tert-butyl ester (step 1) (0.025 g, 0.0964 mmol) in DCM (3 ml) is treated with hydrochloric acid (1 ml, 4M in dioxane) and allowed to stir at room temperature for 1 hour. The reaction mixture is concentrated in vacuo to yield the titled compound as a white solid.

Intermediate CJ3 and CJ4 These compounds namely, 2.[5-(2-Amino-ethyl)-retrazol-2-yl] -ethanol and 3-[5-(2-Aminoethyl) -tetrazol-2-yl]-propan- 1-ol are prepared analogously to intermediate CI2 but replacing the ethyliodide in step 1 with the appropriate haloalcohol and by treating the product with 4M HCI instead of trifluoroacetic acid (step 2).

Intermediate 2-(2-Isop~ropyl-2H--tetrazolI-S-yll-ethylamine This compound is prepared by an analogous procedure to intermediate C12 by replacing the ethyliodide in step 1 with the appropriate haloalkane.

Intermediate CK1 Z-(S-Methvl-tetrazol-2-yl)-ethylamine This compound is prepared by an analogous procedure to intermediate C12 by replacing [2- (1H-Tetrazol-S-yl)-ethyl]-carbamic acid tert-butyl ester in step 1 with 5-Methyl-2H-tetrazole and by replacing ethyliodide with (2-Bromo-ethyl)-carbamic acid tert-butyl ester.

Intermediate CK2 00 2-(5-Cycl opropyl-tetrazol-2-yl)-ethylamine This compound is prepared by an analogous procedure to intermediate GK1 by replacing S-Methyl-2H-tetrazole in step I with 5-Cyclopropyl-2H-tetrazole.

Intermediate CLI 2-Imidazol-1-yl-erhylamine A mixture comprising 2-chloroethylamine hydrochloride (3.00 g, 25.86 mmol), imidazole (1.63 g, 23.94 mmol) tetrabutylamxnonium hydrogen sulfate (0.3 g, cat.), NaOI- (3.45 g, 86.18 mmol) and acetonitrile (75 n-l) is stirred at reflux (810C) under an inert atmosphere for 20 hrs.

The reaction mixture is allowed to warm to room temperature and the suspension is filtered and the solvent is concentrated in vacuo to yield the titled compound as a pale yellow oil.

Intermediate CI 2-Ryrazol-1-yl-etbhylamine This compound is prepared according to the protocol described in A.M. Cuadro, M.P. Matia, J.L. Garcia, J.J. Vaquero, J. Alvarez-Bailla, Synth. Commun. 1991, 21, p53S.

Intermediate CM2 2-(4-Merhyl-p2yrazol-1 -yl)-ethvlamine This compound is prepared using an analogous procedure to intermediate CM1 by replacing 1H-pyrazole with 4-Methyl-1H-pyrazole.

The amine intermediates that are used to prepare the compounds of Examples 121 to are commercially available or are prepared by the procedure that is analogous to that described in Example 121.

The following urea intermediates of formula (D) 0 Br HN N are shown in Table 4 below, the method of preparation being described hereinafter.

TABLE 4 Intermediate

Q

DA -CH 2

CH

2

CH,

DB -CH 2 CHCH2CH 3 DC -CH(CHs)2 DD -CH 2

CH(CH

3 2 DE -CH 2

CH

2 0H DF -CH 2

CH

2 0CH 3 DG *CH 2 CH&H, H Intermediates DA-DG The compounds shown in table 4, namely: 5-Oxo-2-propyl-5,6,7,8-terahydro-imida7o[,S.

cjpyrimidin-2-ium bromide 2-Butyl-S-oxo-5,6,7, 8-tetahydro-iridazo[ 1,5-c)pyrirnidin-2ium bromide 2 -Isopropyl-S-oxo-S,6,7,8tetrahydro-inidazo[1,-cmpyridin-2-iu

(DC),

2-Isoburyl-S-oxo-,6,7,8-tetrahydro-imidazo[1 ,S-cpyrimidin-2-ium bromide 2.(2- Hydroxy-ethyl)-5-oxo-5,6,7,8-tetrahydro-imidazo[1,5-c]pyrimidin-2-ium bromide 2-(2- Methoxy-ethyl)-5-oxo-5,6,7,8-tetrahydro-iniidazo[1,5-c]pyrimidin-2-ium bromide (DF) and 2- (3-Hydroxy-propyl)-S-oxo-5,6,7,8-tetrahydro-imidazo[1 ,5-c]pyrimidin-2-ium (DG) are prepared by alkylation of 7,8-Dihydro-6H-imidazo[1,5-c]pyrimidin-S5one with the appropriate alkyl bromide following the method described in R. Jain and L. A. Cohen, Tetrahedron, (1996), 52, 5363-5370.

Preparation of final compounds Compounds of formula I which are also of formula XI

H

3 C

N

S C-N X111 \R 0

R

are shown in Table 5 below, the method of preparation being described hereinafter. The table also shows mass spectrometry data. The Examples are in free form.

43 TABLE EX. RR4Rb M/S 12 -S0zCH 3 F402.3 o l CH3 13 -SO 2

CH

3 F HC444.1 o A~H 3 14 -SO 2

CH

3 F 'C438.1

N-

is -SO 2

CH

3 F H C 415.1 16 -SO2CH 3 F471.2 17 -SO 2

CH

3 F 388.1

H

3

C"

18 -SO 2

CH

3 F J H 3 416.1.

19 -S02CH3, F (H3 415.1 -S02CH 3 F cjH 3 429.1 ryo CH 3 21 -SOaGE., F 0 3 429.2 22 -SO2CH., F 429.1

NH

2 23 -SO 2 CH., F 0401.2 Ex. R 3 R4Rb M/s 24 -SO 2 GHi T 0 472.2

CH

3 0025 -SO 2

CH

3

-CF

3 471.1

N

1>28 -SO 2

CH

3

-CF

3 522.2

CH

3 28 -SO 2 CI-1 3

-CF

3 438.1

OH

-SO

2

CH

3

-CF

3 4S2.1

OH

31 -SO 2 CH3 -CF 3 452.1 3 32 -SO, 2 CH3 NcF3 S05.2 33 -SO 2

CH

3 -CF3 CH- 3 479.2 34 -SO 2

CH

3

-CF

3 N C 468.1

"CH

3 -SO2zCH3 -CF3 464.1 0 0 Ex. R R RI Mis MIH4 36 -SO 2

CH

3

-CF

3 491.2

CH

3 37 -SO 2

CH

3

-CF

3 L, J H 468.1 47.

38 -SO 2

CH

3

-CF

3 47.

39 -SO 2 CH3I -CF3 H3 O" 521.2 0 -SOzCH3 -CF 3 0 H 3 50.

K Oi'SHCH350

CH

3 41 -SOzCJH3 -CF 3 452.2 0. CH 3 42 -SO 2

CH

3

-CF

3 433.1 43 -SOzCH3 -CF 3 C,503.2 44 -SO 2

CH

3

-CF

3 493.2 K CH3 46 -S0aCH 3

-CF

3 466.1 o"C CH, 48 -S02CH- 3

-CF

3 INS4.1 0

N

CH

2 00 Ex. R3R 4 Rb M/s

MH+

49 -S0 2

CH

3

-GF

3 H 465.1 HC N CHa

-SO

2 CI-b -CF 3 6Cj o.H3 480.2 51 -SO 2

CH

3 H- 398.1 52 -S02CH 3 H 0 453.2 53 SOIGH3 H 0~~A"H 426.2 14 -SOiCH 3 H 'C3 370.1

OH

-SO

2

CH

3 H 356.2

OH

56 -SO 2

CH

3 H 384.2

OH

57 -SOzGH3 H 407.2 0

H

3 58 -S02CH 3 H 0509.2

NH

59 -S02CH 3 H 0'H33 440.2

OH

3

-SO

2 CH3 H C N365.1 48 Ex. R 3 R4Rb Mis MH4.

61 -SO 2

CH

3 CN 0 465.

C

3 62 -SO 2

CH

3 CN 0436.1 N3 63 -SO 2

CH

3 CN 0 422.2 c-I

CH

3 H3 64 F -S02CH. 313 F -S02.CH 3 U N2401 66 F -SO 2

CH

3 j i436 SNs

OH

3 67 F -S02CH 3 441CH

OTH

3 68 F -02.C1H3 374

OH

69 F -SO 2

CH

3 414 0 F -SO 2

CH

3 471

N

0 71 -SO 2

CH

3 cI-0 H 474.1 H3

CH

3 71a *SO 2

CH

3 F H 404.0

_O

Compounds of formula I that are also of formula XIV H 3 C N H

,R

S 0 R H 04 are shown in Table 6 bclow, the method of preparation being described hereinafter. The table also shows mass spectrometry data. The Examples are in free form.

TABLE 6 Ex. RRIM/S

-N/

\R

72 F NN427.1

NH,

7 3 N K N 4 3 6 .1 3 74 F 428.13

N

F 351 76 H "I 409.17 0--

NH

2 77 -CFs -I 477.16 2 78 -CF 3 110459.13

N

Compounds of formula I that are also of formula XV

H

3 C N

H

N N 0 0

H

3 0> o 0 R 4 are shown in Table 7 below, the method of preparation being described hereinafter. The table also shows mass spectrometry data. The Examples are in free form.

TABLE 7 EX. R 4 Het M/s

AM+

79 F 481.1 F '/.CJ440.0 81 F-N 454.2 82 F N468.19 83 F %Kls268.18 84 F 466.13 8S F q H 482.2 86 F rN>,\-C,483.23 0 -N

P~CH

Ex. R4 Het Mv/s 87 FCH 88 F -1440.12

CH,

89 F 454.25

CH,

F 468.16 0' 91 F466.10 92 F 480.14 0' 93 F *~(>482.18 F 438.16 96 F N) 452.17

N

97 F 466.22

CP

2 98 F '..>466.22 99 F 480.25

CF

2

IND

00

IND

Ex. R4 Het M/s

MH+

100 F 480.26

CH,~

101 F TN 482.24

O-CH

102 F 468.24

\-OH

103 F 482.24

OH_

104 F 438.20 105 F CH, 452.17 105a F N 466.23 N

CF

106 F %Ng, CH3 480.26 f~H 2 C CN 107 F 474.89

N-

108 F N 426.18

HN-N

109 F "eN 454.17

H

3 CI/ N-N 110 F TrN454.15 111-CF 502.19 112 cl YNI>JCHJ 470.11 113 CI N 468.18

N

CH Ex. R4 Het M/s

MN.,

114 -CN 460.2 Lf

H

3 115 -CN o CH, 488.10

_CH,

117 1 N 502.10 118 1 N 500.29 119 -CN N 459.24

_CH,

120 H N 448.14 1 Preparation of Specific Examples: The amines that are used to prepare the compounds of the Examples listed in Tables 5 and 6 are commercially available or prepared by standard methods. Some of the amines that are exemplified in Table 3 and are used to prepare the compounds of the Examples listed in Table 7 are not commercially available, the methods of preparation being described above. Several examples in Table 7 are prepared from intermediates that are listed in Table 4.

General Procedure for preparation of Urea examples from Imidazole-urea intermediates (B) and amines (C) The amine (0.12 mmol) in dry DMF (0.12 mmol) is added to a solution suspension of the imidazole urea intermediate (0.11 mmol) in DMF (1.0 ml). Triethylamine may be added to enhance reaction rate and especially if one or both of the starting materials is present as a salt (1.1 equivalents Et 3 N per equiv. salt). The reaction mixture is sonicated if necessary until a clear solution is obtained. The reaction is allowed to proceed at between room temperature and oC until the starting material is consumed (30 minutes to 24 hours). When complete, the 54 mixture is concentrated in vacua to remove the solvent. The product is conveniently purified by dissolving the crude residue in THF (2 ml) and adding this to polymer supported isocyanate (Argonaut Technologies, 0.5 g, 1. 10 mmol) which has been pre-swollen with =H (2 ml). The reaction mixture is allowed to drip through the resin under gravity and the solvent is removed in vacuo to yield the titled compound. Alternatively the product is purified by standard 00 procedure e.g. crystallisation, chromatography or I-ELC.

A typical example is as follows: r- Example 114 0 1-[5-(3-Cyano-4-methanesulfonyl-phenyl-4-methyl-tbiazol-2-yl-3-r2-(5-ethyl-oxazol-2-yl- To a stirred solution of imidazole-1-carboxylic acid'[S-(3-cyano-4-methanesulfonyl-phenyl)-4methyl-thiazol-2-yl]-ainide (Intermediate BD) (0.05 g, 1.29 mmol) in DMI (1.5 ml) under an inert atmosphere is added 2-(S-ethyl-oxazol-2-yl)-ethylamine (Amine (0.018 g, 1.29 mmol) followed by triethylamine (0.02 ml, 0.14 mmnol). The reaction mixture is heated to 700 C for hours and then allowed to cool to room temperatue. The solvent is removed in vacuo and the resulting crude residue is dissolved in TI-F and passed through a 0.5 g polymer supported isocyanate resin (pre-washed with THF). The solution is concentrated in vacua and the crude residue is dissolved in DCM and washed with water, brine, dried over MgSO4 and concentrated in vacua again. Triturating with ether affords the titled compound as a yellow solid.

The compounds of Examples 97-103 are prepared from intermediates DA- DG (Table *A typical example is as follows: Example 97 I -[5-(3-Fluoro--ethanesulfonyl-phenyl)-4-rnethyL- hiazol-2-yll-3-r2-(1-propyl-1H-imidazol- 4.yl-ethy_1l-urrea A stirred mixture of 5-oxo-2-propyl.5,6,7,8-tetrahydro-imidazo[1,5-c]pyrimidin-2-ium bromide (Intermediate DA) (0.214 g, 0.83 mmnol) and S-(3-Fluoro-4-methanesulfonyl-phenyl)- 4-methyl-thiazol-2-ylamine (Intermediate AA) (0.15 g, 0.55 mmol) and Et 3 N (0.48 ml, 3.3 mmcl) is heated at 120 0 C for 2 days. After cooling to room temperature the mixture is diluted with EtOAc (50 ml) and washed water followed by brine. The organic extract is dried (MgSO4) and the solvent is removed to give a solid. Purification by chromatography on silica eluting with DCM: MeOH (95:S) affords the titled compound (0.06 g, The compounds of the till of the other Examples up to and including Example 120 are prepared analogously. These compounds are namely, from Table 5: 1-II-(3-Fluoro-4- IND mehnsloy-hnl--ehltizl2yj3p~ii--lehlue (Ex. 1.fS.(3- Floo4mtaeufnlpey)4mty-ijzl2y]3(-ehx-ty)ue 1- 3 -Fluoro-4-methanesulfonyl-phenyl) 4methyl-thiazol-2-yi]-3-(3-methoxy-propyl) urea 00 1 3 .Fuoro-4-merhanesufony]pheny)mehylrhizol.2-ylI.3-[3(2oxo IND pyrrolidin-1 -yl)-propyl]-urea(Ex.4), 3 -Fluoro-4-methanesulfonyl-phenyl)4-m erthylthiazol- 2 -yl]-3-(2-oxo.tetrahydro-furan-3-yl)urea 1.[S-(3-Fluoro-4-methanesulfonylphnl--ehltizl2yl3-2mtyslay-ty)ue 1-[5-(3-Fluoro-4mehnsloy-hnl--ehltizl2-i--2(-yrx-toy-ty]ue 1- 3 .Fluoro-4-methanesulfonyl-pheny)4methylrhiazol.ay].3.(S.methylj 5 oxazQ- 3 ylmethyl)-urea 3 -Fluoro-4methanesulfonylphenyl)4.methyltbazol2y]3-(I merhyl-lH-pyrrol-2-Armethyl)-urea, 3 -f 3 -[S-(3-Fluoro-4-methanesulfonyi-phcnyi)-4.

merhyl-thiazo-2y]ureido)Nnaphrhalen2ylpropionnide(Ex.10), uoro-4methanesulfonylpheny)4methylthiaoI-2-yl]ureidol-propionic acid tert-butyl ester, (Ex. 11), 1-(2-Ethoxy-ethyl)-a 3 -fluoro-4-methanesulfonyl-phnyl)4methyliazol2$I -urea (Ex. 12), 3 3 .Fluoro-4-methanesulfonyl-phenyl)4methyl Ahazo]-2-yilureido)-acetic acid tert-butyl ester, (Ex.1 1 l,5-Dimethyl-lH-pyrazol3ylmethyl)3[5(3-fluoro-4methanesulfonyl-phenyl)-4-methyl-thiazol-2.yl]-urea (Ex. 14), 2 3 -[-(3-Fluoro..4-mehanesulfony[phenyl )-4-methyl-thiazol-2-yl]-ureidoj -N-methyl-propionamide (Ex.1S), 1 -[S-(3-Fluoro-4mehnsloy-hnl--ehltizl2yl3(-opoi--l3oopoy)ue (Ex. 16), 3 -Fluoro4methanesulfonylphenyl)methyltazo2yl3((S)2hydrox-lmethyl-ethyl)-urea (Ex.17), 3 -Fluoro-4-methanesulfnyphenl)meffyltiaol2yl]- 3 2 -propoxy-ethyl).urea (Ex. 18), 2 i1 3 3 -Fluoro-4-methanesufony-phenyl)A4methyl.thiazol- 2 -yl]-ureido)-N,N-dimethyl-aceramide (Ex. 19), 3 -1 3 3 -Fluoro-4-methanesufonylphenyl)-4 methyl-thiazol.2-yI]-uridoI-N,Ndimethylpropionarade (Ex.20), N-Ethyl-3-( 3-[5-(3-fluoro-4methanesulfonyl-pheny)4methythiaol2yl]ureido-propionaide (Ex.21), 3-(3 Fluoro-4methanesudfonyl.phny)4metyl-tazol.2yl]ri..ure2-dim ethy..piaide (Ex.22), 3-3[-3Fur--ehnsloy-hnl--ehltizl2y]ued)poin amide 3 -1 3 3 Fluoro4-methanesufonyipheny)4methylthiazol2yl]-ureido)- 2 methyl-propionic acid tert-butyl ester (Ex.24), 1 4 -Methanesulfonyl-3-trifluoromethylphenyl)-4-methyl-thiazol2ylI3-pyridin-2-ymethyl{urea (Ex2S), N-(2-diniethylaniino-ethyl)-3 13[-4mtaeufnl3tilooehlpenl--ehltizl2y]uedlpoin arnide (Ex.26), 4 -Methanesulfonyl3trifluoromethylphenyl)4my-taol.

2 -ylj- 3 pyridin-3-ylmethyl-urea (Ex.27), 1-IS-( 4 -Methanesulfonyl3trifluoromtyl-phenyI)-4methyl thiazol-2-yl]-3-(2-methoxy..ethyl).urea (Ex.28), 1-( 2 -IHydroxy-ethyl)-3[S-(4.methn-sulfonyl.

lu ~3-trifluoromethyl-phenyl).4-methylthiazol2y]urea (x2)1( (Ex29) 1-1ydroxy-but-yl mehnsloy--rfurmty-hey)4mty-'izl2y]ue (Ex.30), 1 (Ex.31), 4 -Methanesulfony3fluoromehylphenyl)4merhythizo-2yl] 3 3 2 -o pyrrolidin- 1-yl)-propyfl-urea (Ex.32), l-( 2 -Diethylamino-ethyl)-3-[5-(4..methanesulfonyl-3 00 trifluoromethyI-phenyl)A4-methylthiazo2ylurea (Ex.33), 1 -[S-(4-Methanesulfonyl-3-tri- IND ~fluoromethyl-pheny)4methythiazo2y]3(3-methylsulfanyl-propyl)..rea (Ex.34), Mehnsloy--rfurmry-hnl--ehltizl2y]3(-x-erhdofrn 3-yl)-urea (Ex.35), 4 -Methanesulfonyl3trifluorometyi-phenyl)4methy-thjazol-2-y] 3 2 -(l-methyl-pyrrolidin-2-y)-ethyl].urea (Ex,36), l-[2-(2-Hydroxy-ethoxy)-ethyl]-3[S-(4.

mehns~oy--rfurmty-hnl--ehltizl2y]ue (Ex.37), 1-f S-(4- Methanesulfonyl-3-trifluorornerhylpbenyl)4-methyI.tjhazo-2yl- 3 1-methyl-1H-pyrro]-2ylmethyl)-urea (Ex.3 4 -MethanesufonyI-3trifluoromethylphenyl)4methyI-thiazol- 2 yII- 3 3 -morpholin-4-yl-3-oxo-propyl).urea (Ex.39), 3 3 -[S-(4-Methanesulforiyl-3-trifluoromerhyl-phenyl)A4-rehyltiazol2yi]-ureido)-propionic acid tert-butyl ester (Ex.40), 1-(2- Ethoxy-ethyl).3[S(4methanesufony3trfluoromehylphenyl)4mehy-thizo- 2 -yi-r (Ex.41), l-( 2 -Cyano-ethyI)-3-[5-(4methanesufonyl3-trifluoromethyi..phenyI)-4-methylthiazol-2-y!J-urea 4 -Methanesufonyl-3-rifluoromehy.phenyl)A..methylrhiazol-2-ylJ-3-(8 -methyl-8-aza-bicyclo[3 l]oct-3-yl)-urea (Ex.43), N-(4-{3-[5-(4-MethanesulfonyI-3trifluoromehypheny)-mehythiazo-2yl]ureido)-butyI)-acetamide (Ex.44), -[S-(4MehanesulfonyI3trifluoromehy-phenyl)4methyI-thiazol-2y1]-ureido)propionic acid methyl ester (Ex.45), 2 3 -[S-(4-McthanesulfonyI..3-trrfluoromethyl-phenyl).

4 -methyl-thiazol-2.yl]-ureidol-propianic acid methyl ester (Ex.46), 13-[5-(4-Methanesulfony1-3trifluoromethy-phenyI)A4-methylthiazol.2.yII.ureido)..acetc acid tert-butyl ester (Ex.47), 1-5(-ehnsloy--rfurmty-hey)4mty-i zl2y]3p(-ehl piperazin-1 -yl)-3-oxo-propylj-urea (Ex.48), 2 -{3-[S-(4-Methanesulfony[-3.trifluoromethyl.

phenyl)-4-methyI-thiazoJ2yi]ureido)Nmethylpropionamide (Ex.49), (S)-2-f3-75-(4- MethariesulfonyI-3-trifluoromehylphenyl)4methyl-thazoI-.yl]yureido)-propionic acid ethyl ester (Ex.SO), (S--3[-4Mtaeufnlpey)4mty-hao--l-rio-rpoi acid methyl ester (Ex.S 1-1S-( 4 -Methanesulfonylpheny)4mehythiazo2yl- 3 3 morpholin..4-yi-3-oxo-propyl)-urea 3 4 -Methanesulforiyl-phenyl)-4-methyl.

thiazol-2-yl]-ureido).acetic acid tert-butyl ester (Ex.S3), l-(3-Hydroxy-propyl)-3[5-(4rnethanesulfonyJ-phe nyl)Amethy-thiazoI2y]urea (Ex.54), 1 -(2-Hydroxy-ethyl)-3-[5-(4methanesulfony]-phenyl)4mehyl-thiao.2yl].urea (Ex.55), 1 -(4-Hydroxy-buy).3.[s.(4.

methanesulfonyJ-phenyl).4methyI.thiazoy.2yl]-urea (Ex.56), l-LI-( 4 -Methanesulfanyl-phenyl)- 4-inethyl-thiazol-2yl]3-(5-methyl-isoazol-3-ylmethyI)-urea (Ex.5 3-{3-[5-(4-Methanesulfonyl-pheny)4methylthazo-2-yl]-ureido)-Nnaphthalen-2yl-propiona1ide (Ex.S 8), 3-[-4Mtaeufn phnl--ehltizl--l-rio-rpo acid tert-buty] ester (Ex.59), 1-2Caoehl--S(-ehnsloy-hnl--ehltizl2y]ue 3 {3.[5.(3..Cyano-4-methanesufonyphel)-4-methythiazo[2-yI-ureidoV-propionic 00 acid tert-butyl ester (Ex.61), 3 -13 [-3Cao-ehneufnlpey)4-ehltizl yIJ-ureido)-NN-dimethyl-propionamide (Ex.62), 2-{3-[-(3-Cyano-4-methanesufoflyl-phelyl)- 4-methy1-thiazol-2.ylI.ureido)-N,N-dimethylacetamide (Ex.63), 1-(2-Cyano-ethyl)-3-[5-(4fluoro-3-methanesulfony-phenyl)-4-methy]-thiazol-2-yl]urea (Ex.64), 3-13-[S-(4-Fluoro-3methanesulfony-phenyl)-4-methyl-thiazo-2yl-ureido)-propiofamnide (Ex.65), 1-[S-(4.Fluoro- 3-ehnsloy-hnl--ehltizl2y]3(-ehnsloy-ty)ue (Ex.66), 13- [5(-loo3mtaeufn-hnl--ehltizl2y]uedlaei acid -tert-butyl ester (Ex.67), 1-[5-(4-Fluoro-3 .methanesulfonyl-pheny1)-4-methyl-thiazoI-2-ylF 3 2 -hydroxyerhyl)-urea (Ex.68), 1-S(-loo3mtaeufnlpey)4mty-hao--l--2oo tetrahydro-furan-3-yl)-urea (Ex.69), 1-[S-(4-Fluoro-3-methanesulfonyl-phenyl)-4-methylthao--l--3mrhli--l3oopoy)ue (Ex.70), 3-(3-[5-(3-Chloro-4-methanesufnlpey)4mty-hao--l-rio-rpoi acid tert-buryl ester (Ex.71), and 1- (23Dhdoypoy)3[-3fur--ehnsloy-hnl--ehltiz12ylue (Ex. 71a); from Table 6: Pyrrolidine-1,2-dicarboxcylic acid 2-amnide 1-1[S-(3-fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazo-2-yllamide) (Ex.72), 1,4,6,7-Tetrahydro-imidazoI4,S-c]acid [5(-loo4mtaeufnlphnl--ehltizl2y] amide (Ex.73), 1-5(-loo4mtaeufnlpeyl--ehltizl2yc*abmyl azetidine-3-carboxylic acid methyl ester (Ex.74), 3-Gyano-azetidine-1-carboxylic acid fluoro-4-methanesulfonyl-pheny1)-4methyl-tiazol2-yl]-aride (Ex.75), Pyrrolidine-1 ,2dicarboxylic acid 2-amide 1-I [S-(4-methanesulfonyl-phenyl)-4-methyl-thiazo[-2-yl]amide) (ExJ76), 1 (-yrx-ty)3[-4mtaeufny--rfurmty-hnt--ehl thiazol-2-yl]- 1-methyl-urea (Ex.77), 2-Cyano-pyrrolidine-1-carboxylic acid [5-(4-methanesulfonyl-3-trifluoromethyl-phenyl)-4-methyl-thiazol-2-yI]-amide (Ex.78) and (S)-Pyrrolidine- 1 ,2-dicarboxylic acid 2-arnide [S-3-imiddazol-1 -yl-4-methanesulfonyl-phenyl)-4-methylthiazol-2-yl]-amidel (Ex.78a); and from Table 7: 1-[2-(5-tert-Buryl-oxazol-2-yl)-ethyll-3-[S-( 3 fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yiI41irea (Ex.79), 1-[S-(3-Fluoro-4mehnsloy-hnl--ehltizl2y]--2(-ehl[,,]xdao--i-ty] urea (Ex.8 1-[2-(3-Ethyl-[1 ,2,4]oxadiazol-5-yl)-ethyl-3-[5-(3-fluoro-4-methalesllfofylphenyl)-4-methyl-thiazol-2-yI]-urea (Ex.81), 1 -Ethyl-[1,2,4]oxadiazol-S-yl)-ethyl]-3-[S-(3fluoro-4-methanesulfonyl-pheflI)-4-methyl-thiazoI-2l]burea (Ex.82), 1-[S-(3-Fluoro-4methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]-3-[ 2 3 -isopropyl-[1,2,4]oxadiazol-5-y)-ethyllurea (Ex. 83),l1-[2-(3-Cyclopropyl{1 2 4 ]oxadiazol-S-yl)-ethyl]-3-[5-(3-fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]-urea (Ex. 84), 1 -[2-(3-tert-Butyl-[1 2 cthylI-3-[-(3-fluoro-4-mehanesulfonyl-phenyl)4.methyl-thiazol..2.yly-urea (Ex.8 Dimethylaminomethyl-[1, 2 4 ]oxadiazol-S-y!)-erhyl]-3-[S-(3-fluoro-4-methanesuffonyl-pheny 4-mechyl-thiazol-2-yll-urea (Ex. 86), I-[S-(3-FluoroA4-methanesulfonyl-phenyl).4-methyl 00 thiazol-2-yl]-3-[2-(3 -methyl-fl ,2,4]thiadiazol-5.yl)-ethylj-urea (Ex. 87), 1-[S-(3-Fluoro-4- IND methanesulfony1-phenyI)-4-methyI-thiazoI.2-yI).3-[2-(5-methyl[1 ,3 oxadiazol-2-yl)-ethyl]- 0 urea (Ex. 88), l-[ 2 -(5-ErhyI-[1,3,4].oxadiazo-2yl)ethyI-3-[5.(3-fluoro-4.methansufonyphenyI)-4-methyl-thiazol-2-ylI-urea (Ex. 89), l-[5-(3-Fluoro-4-mehanesulfonyl-phenyl)-4methyl-thiazol- 2 -yl]-3-12-(5-isopropyl-[1,3,4oxadiazoI..2-yi)..thyl]-urca (Ex.90), 1 r~l Cyclopropyl-f 1 3 4 Ioxadiazoi- 2 -yI)-ethyl-3.[S(3-fluoro-4methane-sulfonyi-phenyI)-4methy..

thiazol-2-yiI-urea (Ex.91), 1 -[2-(S-Cyclo-butyl-[l ,3,4]oxadiazol-2-yI)-ethyll-3-[S.(3 -fluoro-4methanesulfonyl-phenyl)A4-mehythiazol2yl]-urea (Ex.92), 1-[2-(S-tert-Butyl-[1 oxadiZI2y)ehl--S(-loo4mtaesloy-hnl--ehltizl2y]ue (Ex.93), 1 -[S.(3-Fluoro-4-methanesulfonyl-phenyI)-4mthyl.thiazol.2.yI].3-2-( H-imidazol-4yI)-ethyll-urea (Ex.94), 3 -Fluoro-4-methanesulfonyl-phenyl)-4-methylrthiazolp2yJ.3[2- (1-methyl-1H-imidazol-4-yl)-erhyl].urea (Ex.95), I 1-Ethyl-1H-imidazol..4-yl)-ethyl]-3 4S 3 -fluoro-4methanesufonylphenyl)4methytzoI-2-yi]-uea (Ex. 96), 1-[5-(3-Fluoro-4methanesulfonyl-pheny)A4-methyl-thiazob2-yll.3[2.(l1-propyl-1H-imidazol-4-yl)-ethyl]-urea (Ex.97), l-[S-(3-Fluoro-4-methanesulfonyi-pheny1)-4methy-thazo1-2..yI].3-2-(1-isopropyllI--imidazol-4-yi)-ethyl]-urea (Ex.98), 1 -[2-(l1-Buryl-lH-imidazol-4-yl)-ethyl]-3[S(3fluoro-4.

methanesulfonyl-phenyl)-4-niethyl..thiazol-2-y].urea (Ex.99), 1-[S-(3-Fluoro-4-methanesulfonyl-phenyl)-4-methy-thazo2yl..3[2-(1-isobutyl-1H-imi dazol-4-yl).erhyll-urea (Ex. 100), 3 -Fuoro- 4 -mehanesulfonypheny)4mehythio2y]32[12methoxy ethyl)-lH-imidazol.4-yl]-ethyl)-urea (Ex.1 01), 1-[S-(3-Fluoro-4-methanesulfonyl-phenyl)-4 merhyl-thiazo1.2-ylJ-3-12.[1 2 -hydroxy-ethyl)-1H-imidazol-4-yIJ..ethyl)-urea (Ex. 102), Floo4mtaeufnlpey)4mty-hizl2yl3(-I(-yrx-rpl-H imidazol-4-yI]-ethyl}-urea (Ex. 103), 3 -Fluoro-4-metbanesulfonyl..phenyl)-4methylthiazoI- 2 -yIJ-3-[2-(3-methyl-3H-inidazol1Ay)..ethyl]-urea (Ex. 104), 1-[2-(3-Ethyl-3Himdzl4y)ehl--5(-loo4mtaesloy-hnl--ehltizl2y]ue (Ex. 105), 1-S(-loo4mtaeufnipey)4-ehltiz12yl3[-2iorpl 3H-imidazol-4-yI)-ethyl]-urea (Ex. 1 Oa), l-[2-(2-tert-Buryl-1H-imidazol-4-yI)-ethyl]-3-[5-(3 fluoro-4-methanesulfonyl-phenyl 4 -methyl-thiazol-2-yi].urea (Ex.1 06), 1 -[2-(lH--Benzoiriao--l-ty]3[-3fur--ehneufnipey)4mty-ha~l2y]ue (Ex. 107), 1-S(-loo4mtaeufnlpey)4-ehltizl2y]3[-l-erzl5 yl)-ethyl]-urea (Ex.10 1 I-Ethyl-1N-tetrazo1-5.yI)-ethyl13[5(3.fluor6A-4methane 59 (1)sulfonyl-phenyl)-4-methyl-thiazol-2-yl]-urea (Ex.109), 1 -[2-(2-Ethyl-2H-tetrazol-5 -yI)-ethyl]-3- 3-fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-ylI-urea (Ex. 110), 1-Ethyl- 1H--imidazol-4-yl)-ethyl)-3-[S-(4-methanesulfonyl-3-trifluoro-methyl-phenyl)-4-methyl-thiazol- 2-ylII-urea (Ex.11 1-[S-(3-Chloro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl-3-[2-(3echyl-[1 ,2,4]oxadiazo!-S-yl)-ethylI-urea (Ex. 112),l1-[S-(3-Chloro-4-methanesulfonyl-phenyl)-4- 00 methyl-thiazol-2-yI]-3- 1 -ethyl -1 H-imidazol-4-yl)-ethylJ-urea (Ex.1 13), 1-[S-(3-Cyano-4methanesuifonyl-phenyl)-4-methyl-thiazol-2-yl]-3-[2-(5-ethyl-oxazol-2-y)-ethyll-urea (Ex.1 14), 1 -[2-(S-tert-Butyl-oxazol-2-yl)-ethyll-3-[S-(3-cyano-4-methanesulfonyl-phenyl)-4-methylthiazol-2-yl]-urea (Ex.1 15), 1-[2-(5-Erhy]-oxazol-2-yl)-ethyl]-3-[S-(3-imidazol-1-yl-4-methanesulfonyl-phenyl)-4-rnethyl-thiazol-2-yl]-urea (Ex. 116), 1 -[2-(3-iEthyl-[1,2,4]oxadiazol-S-y)ethyl] [5-(3-imidazol-1-yl-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]-urea (Ex. 117), 1- [2-(l-Ethyl-lH-imidazol-4-yl)-ethyl]-3-fS-(3-imridazol-1-yl-4-rriethanesulfonyl-phenyl)-4methyl-thiazol-2-yl]-urea (Ex.1 18), 1-[5-(3-Cyano)-4-methane-sulfonyl-phenyl)-4-methylthiazol-2-yl]-3-[2-(1 -ethyl-i H-imidazol-4-yl)-ethyl]-urea (Ex. 119), and 1-[S-(4-Methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]-3-[2-(1-propyl-1H-imidazol4-yl)-ethyl]-urea (Ex. 120).

Compounds of formula I that are also of formula XVI

H

3 C N H S

XV

~0 F are shown in Table 8 below, the method of preparation being described hereinafter. The table also shows mass spectrometry data. The Examples are in free form.

TABLE 8 Ex. Rb M/s

MH+

121 0%457.1 122 ll C 474.2 123 G%.0 446.2 Ex. Rh 124 N-N 00 125 126 0 Example 121 N-tr-UJ31-5(-loo4reh"pjo~jhryD4m ltizl2yluedl propionamide Src.L 2 -ier-Butylcarbamoy-rhy)-carbamic acid tert-butyl ester: A solution of 3-tert-Butoxycarbonylamino-propionic acid (0.5 g, 2.64 mmol) in DCM (5 mi) under an inert atmosphere is treated with 1-hydroxy-7-azabenzatriazole (0.108 g, 0.79 mmol) followed by water soluble carbodiimide (0.491 g, 3.17 mmol) and tert-butylamine (0.305 ml, 2.91 mmol). The reaction mixture is stirred at room temperature for 3 hours and then citric acid (10 ml, 0.5 M) is added to the solution and extracted with DCM (2 x 10 ml). The 00 combined organic layers are washed with brine (10 ml) and dried over MgSO4. After

\O

filtration, the solvent is removed in vacuo to yield the titled compound as an oil.

S=L 3-Amino-N-tert-butyl-propionamide: O (2-tert-Buylcarbamoyl-ethyl)-carbamic acid tert-butyl ester (0.65 g, 2.64 mmol) is dissolved in C trifluoroacetic acid (3.5 mi) and allowed to stir at room temperature under an inert atmosphere for 3 hours. The solvent is removed in vacuo to yield the titled compound as a TFA salt which is used crude in the next step: S N-tert-Butyl-3-3-[-(3-fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-ylureido)-propionamide: To a stirred solution of 3-amino-N-tert-butyl-propionamide (0.075 g, 0.29 mmol) (step 2) in DMF (2 ml) is added triethylamine (0.092 ml, 0.66 mmol) followed by imidazole-1-carboxylic acid [5-(3-fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]-aide (0.1 g, 0.26 mmol).

After stirring at room temperature over night, the solvent is removed in vacuo and the residue is dissolved in ethyl acetate (10 ml). This organic portion is washed with water (2 x 10 mi), brine (1 x 10ml). After drying (MgSO 4 the mixture is absorbed on silica and purified by chromatography, eluting with ethyl acetate to give the titled compound.

The compounds of Examples 122 to 154 are prepared analogously using the general procedure for preparation of urea examples from imidazole-urea intermediates and amines that is detailed above.

These compounds of Examples 122 to 154 are namely, 1-(4,4-Diethoxy-butyl)-3-[5-(3-fluoro- 4-methanesulfonyl-phenyl)-4-methyl-thiazol- 2 -yl-urea (Ex.122), 1-(4,4-Dimethoxy-butyl)-3- [S-(3-fluoro-4-methanesulfonyl-pheny)-4-methyl-thiazol-2-yl-urea (Ex.123), 1-[S-(3-Fluoro-4methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]-3-(1H-tetrazol-5-ylmethyl)-urea (Ex.124), N- (2,2-Dimethyl-propyl)-3-(3-[S-(3-fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]ureido)-propionamide (Ex.125), 3-{3-[5-(3-Fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol- 2-yl]-ureido)-N-methyl-propionamide (Ex.126), 3-(3-[5-(3-Fluoro-4-methanesulfonyl-phenyl)- 4-methyl-thiazol-2-yl]-ureido)-N-isobutyl-N-methyl-propionamide (Ex.127), 3-(3-[5-(3-Fluoro- 64 4rnethanesulfony1-phenyl)-4-methy-thiazo-2-y]-ureido)-N-isopropy-N-met1y-propioflalide (Ex.128), N-(3-Dimethylamino-2,2-dimethyl-propyl)-3-{3-[5.(3-fluoro-4-methanesulfonylphenyl)-4-methyl-thiazol-2-yI]-ureido)-propionamide (Ex. 12) N~r-uy-.34S-(3-fluoro- 4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yJ]-ureido)-acetamide (Ex.1 30), 1 .(2-Ethyl-2Htetrazol-5-ylmethyl)-3-S-(3-fluoro-4-mehanesulfonyl-phenyl)-4-methy-thiazo-2-y11-urea 00 (Ex.1 31), 1 -Ethyl- 1I-t-erazol--ylmethyl)-3-[-(3-fiuoro-4-methanesulfonyl-phenyl)-4methyl-thiazol-2-yl]-urea (Ex. 132), 1,1.Dimethyl-propyl)-2-{3-[S-(3-fluoro-4-inethanesulforiyl-phenyl)-4-methyl-tbiazol-2-yl]-ureido)-acetamide (Ex. 133), 2-{34[5-(3-Fluoro-4methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]-ureido)-N-methyl-N-propyl-acetanhide (Ex, 134), 2-(3-[S-(3-Fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yI)-ureido-Npropyl-acetamide (Ex.1 35), N-(2,2-Dimethyl-propyl)-2-{3-IS-(3-fluoro-4-methanesulfonylphenyl)-4-methyl-thiazol-2-yI]-ureido)-acetamide (Ex. 136), N-tert-Buryl-2-13-[5-(3-fluaramethanesulfonyl-phenyl)-4-methyl-thiazol-2-yII-ureidol-N-rnethyl-acetamide (Ex.1 37), 2-13-[S- (3-Fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yI]-ureido)-N-isopropyl-N-methylacetamide (Ex.1 38), 1 -[S-(3-Fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]-3-(2morpholin-4-yI-2-oxo-ethyl)-urea (Ex.139), N-Ethyl-2-13-[S-(3-fluoro-4-methanesulfonylphenyl)-4-methyl-thiazol-2-yl] -ureidol-N-methyl-acetrmide (Ex.1 40), 1-[5-(3-Fluoro-4methanesulfonyl-phenyl)-4-methy!.rhiazol-2-yI]-3-(5-isopropyll ,3,4)oxadiazol-2-ylmethy!)urea (Ex. 141), N-(3-Diniethylamino-2,2-dimethyl-propyl)-2- 3 -[5-(3-fluoro-4-methanesulfonylphenyl)-4-rnethyl-thiazol-2-yI]-ureido)-acetamide (Ex. 142), 2-13-[S-(3-Fluoro-4-methanesulfonyl-phcnyl)-4-methyl-thiazol-2-yI]-ureido)-N-isobutyl-N-methyl-acetamide (ix. 143), 1-CS- Ethyl-[ 1,3,4]oxadiazol.2-ylrnethyl)-3-[5-(3-fluoro-4-methanesufonyl-phenyl)-4-methyl-thiazol- 2-yII-tirea (Ex. 144), 1,1-Dimethyl-propyl)-3-(34[5-(3-fluoro-4-methanesulfonyl-phenyl)-4methyl-thiazol-2-yl]-ureido)-propionamide (Ex.145), N-(2-Dimethylamixio-1, 1-dimerhyl-ethyl)- 3-1345S-(3-fluoro-4-methanesulfonyl-phenyl)4-methyl-rhiazol-2-yl]-ureido)-propionamide (Ex. 146), 1-[3-(4,4-Dimethyl-oxazolidin-3-yl)-3-oxo-propyll-3-[S-(3-fluoro-4-methanesulfonylphenyl)'4-methiyl-thiazol-2-yI]-urea (Ex.147), 1-[S-(3-Fluoro-4-methanesulfonyl-phenyl)-4methyl-thiazol-2-yI]-3-[2-(S-methyl-tetrazol-2.yl)-ethyl]-urea (Ex.1 48), 1-[2-(5-Cyclopropyltetrazoi-2-yl)-ethyll-3-[S-(3-fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]-urea (Ex. 149), 14S-(3-Fluoro-4-methanesulfony-phenyl)-4-methyl-thiazol-2-y l-3-[2-(2-isopropyl- (Ex. 150), 1 -[S-(3-Fluoro-4-methanesulfonyl-phenyl)-4-methylthiazol-2-yl]-3-(2-imidazoi-1 -yl-ethyl)-urea (Ex. 151), 1 -[5-(3-Fluoro-4-methanesulfonylphenyl)-4-methyl-thiazol-2-yI]-3-{2.[1-((S).3-hydroxy-2-methyl-propyl)-lH-imidazol-4-y]ethyl)-urea (Ex.152), 1-[5-(3-Fluoro-4-methanesulfonyl-phenyl)-4-methyl-thiazo-2-y]-3-{2-11- (3-hydroxy-butyl)-lH-imidazol-4-yI]-ethyl)-urea (Ex. 153), And 1 -[5-(3.Fluoro-4-methanesulfonyl-phcnyl)-4-methyl-thiazol-2-yl]-3-[2-(4-methyl-pyrazol-1-yl)-ethyl]-urea (Ex. 1S4).

64A IND The reference in this specification to any prior publication (or information derived from it), or to any matter which is known, is not, and should not be taken as an acknowledgment or admission or any form of suggestion that that prior publication (or information derived 00 IND from it) or known matter forms part of the common general knowledge in the field of 0 0 5 endeavour to which this specification relates.

Claims (5)

1. A compound which is 1-[2-(2-Ethyl-2H-tetrazol-5-yl)-ethyl]-3-[5-(3-fluoro-4- O methanesulfonyl-phenyl)-4-methyl-thiazol-2-yl]-urea, in free or salt form. oO 005 O S2. A pharmaceutical composition comprising the compound according to claim 1, in free or salt form.

3. Use of a compound according to claim 1 in the manufacture of a pharmaceutical composition for the treatment of a disease mediated by phosphatidylinositol 3-kinase.

4. Use according to claim 3 where the disease is respiratory diseases, allergies, rheumatoid arthritis, osteoarthritis, rheumatic disorders, psoriasis, ulcerative colitis, Crohn's disease, septic shock, proliferative disorders such as cancer, atherosclerosis, allograft rejection following transplantation, diabetes, stroke, obesity or restenosis. Use according to claim 3 where the disease is asthma, rheumatoid arthritis, proliferative disorders such as cancer or allograft rejection following transplantation.

6. A process for the preparation of the compound as defined in claim 1 which comprises the steps of: reacting a compound of formula II N NH S\S 0 F II wherein T is a 5- or 6-membered heterocyclic ring having one or more ring hetero atoms selected from the group consisting of oxygen, nitrogen and sulphur, with a compound of formula III P OPERUASU0177078 div clams doc 16 02/2)7 -66- H 2 N N"N N-N under conditions known per se; reacting a compound of formula IV N 0 S OF IV with a compound of formula III under conditions known per se; reacting a compound of formula V N o ,-NH 2 OF V with a compound of formula VI OC 'N NON Ii N-N SVI under conditions known per se; and (ii) recovering the resultant compound.

7. The compound according to claim 1 substantially as hereinbefore described with reference to Example 110. P:AOPERXASUOI77078 div c~smmidw.I6102/207 -67- IND 8. A process according to claim 6 substantially as hereinbefore described with reference to any one of the Examples. 0