AU2006214319A1

AU2006214319A1 - Fused heterocyclic compounds useful as inhibitors of histone deacetylase

Info

Publication number: AU2006214319A1
Application number: AU2006214319A
Authority: AU
Inventors: Sampath K. Anandan; Dinesh V. Patel; John S. Ward; Xiao-Yi Xiao
Original assignee: MIKANA THERAPEUTICS Inc
Current assignee: Miikana Therapeutics Inc
Priority date: 2005-02-14
Filing date: 2006-02-14
Publication date: 2006-08-24
Also published as: JP2008530136A; EP1851219A1; AU2006214319A8; CA2596015A1; US20060199829A1; US7772245B2; WO2006088949A1

Description

WO 2006/088949 PCT/US2006/005312 FUSED HETEROCYCLIC COMPOUNDS USEFUL AS INHIBITORS OF HISTONE DEACETYLASE CROSS-REFERENCE TO RELATED APPLICATION This application claims the benefit under 35 U.S.C. 119(e) to co-pending provisional 5 application U.S. Serial No. 60/652,870 filed on February 14, 2005, which is incorporated herein by reference in its entirety. BACKGROUND OF THE INVENTION Field of the Invention This invention relates to compounds which inhibit histone deacetylase (HDAC) 10 enzymatic activity. This invention is also directed to pharmaceutical compositions comprising such compounds as well as to their use to treat conditions, particularly proliferative conditions, mediated at least in part by HDAC. References The following publications are cited in this application as superscript numbers: 15 1 Marks, et al., Nature Reviews: Cancer 1:194-202 (2001) 2 Finnin, et al., Nature, 401:188-193 (1999) 3 Geerts, et al., European Patent Application Publication No. 20 0 827 742, published March 11, 1998 State of the Art In all eukaryotic cells, genomic DNA in chromatin associates with histones to form nucleosomes. Each nucleosome consists of a protein octamer made up of two copies of 25 each histone: H2A, H2B, H3 and H4. DNA winds around this protein core, with the basic amino acids of the histones interacting with the negatively charged phosphate groups of the DNA. The most common posttranslational modification of these core histones is the reversible acetylation of the 8-amino groups of conserved highly basic N-terminal lysine residues. The steady state of histone acetylation is established by the dynamic equilibrium WO 2006/088949 PCT/US2006/005312 between competing histone acetyltransferase(s) and histone deacetylase(s) herein referred to as HDAC. Histone acetylation and deacetylation has long been linked to transcriptional control. The recent cloning of the genes encoding different histone acetyltransferases and histone deacetylases provides a possible explanation for the relationship between histone 5 acetylation and transcriptional control. The reversible acetylation of histones can result in chromatin remodeling and as such act as a control mechanism for gene transcription. In general, hyperacetylation of histones facilitates gene expression, whereas histone deacetylation is correlated with transcriptional repression. Histone acetyltransferases were shown to act as transcriptional coactivators, whereas deacetylases were found to belong to 10 transcriptional repression pathways. The dynamic equilibrium between histone acetylation and deacetylation is essential for normal cell growth. Inhibition of histone deacetylation results in cell cycle arrest, cellular differentiation, apoptosis and reversal of the transformed phenotype. Therefore, HDAC inhibitors can have great therapeutic potential in the treatment of cell proliferative 15 diseases or conditions.' The study of inhibitors of histone deacetylases (HDAC) indicates that indeed these enzymes play an important role in cell proliferation and differentiation. The inhibitor Trichostatin A (TSA) causes cell cycle arrest at both the GI and G2 phases, reverts the transformed phenotype of different cell lines, and induces differentiation of Friend leukemia 20 cells and others. TSA and sub-roylanilide hydroxamic acid ( SAHA) have been reported to inhibit cell growth, induce terminal differentiation, and prevent formation of tumors in 2 mice. Trichostatin A has also been reported to be useful in the treatment of fibrosis, e.g., liver fibrosis and liver chirrhosis.

3 25 In view of the above, there is an ongoing need for inhibitors/antagonists of HDAC. SUMMARY OF THE INVENTION This invention is directed to compounds, compositions, and methods for treating diseases mediated, at least in part, by histone deacetylases. 2 WO 2006/088949 PCT/US2006/005312 Specifically, this invention is directed to compounds, stereoisomers, tautomers, or pharmaceutically acceptable salts of formula I and the related compositions and methods: A N-W -Ar 2 -G4 W NHOH 5 wherein: is Ar 1 which is selected from the group consisting of substituted 1,2-fused aryl, 1,2 fused heteroaryl, substituted 1,2-fused heteroaryl, 1,2-fused heterocyclic; and substituted 1,2-fused heterocyclic; 10 Ar' is fused to the ring containing W1 and W; W and W1 are independently [-C(R)(R 2 )-]m;

W

2 is a bond or [-C(R1)(R2 R' and R2 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and 15 substituted heterocyclic; each m is independently 1 or 2; and p is 1, 2, 3, or 4; Ar2 is selected from the group consisting of arylene, substituted arylene, heteroarylene, and substituted heteroarylene; and 20 G is selected from the group consisting of a bond, alkylene, substituted alkylene, alkenylene, substituted alkenylene, alkynylene, and substituted alkynylene; provided that when Ar 2 is arylene or substituted arylene, then G is not alkenylene or substituted alkenylene. DETAILED DESCRIPTION 25 Throughout this application, the text refers to various embodiments of the present compounds, compositions, and methods. The various embodiments described are meant to provide a variety of illustrative examples and should not be construed as descriptions of 3 WO 2006/088949 PCT/US2006/005312 alternative species. Rather it should be noted that the descriptions of various embodiments provided herein may be of overlapping scope. The embodiments discussed herein are merely illustrative and are not meant to limit the scope of the present invention. In one embodiment, the invention provides a compound of formula I, a stereoisomer, 5 tautomer, prodrug, or pharmaceutical acceptable salt thereof: 1/ 0 A N-W2-Ar 2 -G Wj NHOH wherein: 10 is Ar 1 which is selected from the group consisting of substituted 1,2-fused aryl, 1,2 fused heteroaryl, substituted 1,2-fused heteroaryl, 1,2-fused heterocyclic, and substituted 1,2-fused heterocyclic; Ar' is fused to the ring containing W1 and W; W and W1 are independently

[-C(R')(R

2 )-m; 15

W

2 is a bond or [-C(R')(R2 R and R2 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic; each m is independently 1 or 2; 20 p is 1, 2, 3, or 4 ; Ar2 is selected from the group consisting of arylene, substituted arylene, heteroarylene, and substituted heteroarylene; and G is selected from the group consisting of a bond, alkylene, substituted alkylene, alkenylene, substituted alkenylene, alkynylene, and substituted alkynylene; 25 provided that when Ar 2 is arylene or substituted arylene, then G is not alkenylene or substituted alkenylene. In one embodiment, Ar' is 1,2-fused heteroaryl, substituted 1,2-fused heteroaryl, 1,2-fused heterocyclic, or substituted 1,2-fused heterocyclic. In some aspects of the 4 WO 2006/088949 PCT/US2006/005312 embodiment, Ar and the ring containing W1 and W to which it is fused together form the optionally substituted groups in the following table: Structure Name H 1,3,4,9-tetrahydro-2H-b-carbolin-2-yl N N H 1,3,4,4a,9,9a-hexahydro-2H-b-carbolin-2 H NA y 1,3,4,5-tetrahydro-2H-pyrido[ 4

,

3 -b]indol N 2-yl N H 1,1a,3,4,4a,5-hexahydro-2H-pyrido[4,3 N b]indol2-yl N H -A 1,4,5,6-tetrahydroazepino[4,5-b]indol N 3(2H)-yl N H 3,4-dihydro[1]benzothieno[2,3-c]pyridin S NJ 2(1H)-yl 3,4-dihydro[1]benzofuro[ 2

,

3 -c]pyridin 'NJ 2(1T)-yl 0 10-oxo-3,4,5,10-tetrahydrobenzo[b]-1,6 NA naphthyridin-2(1IH)-yl. H 5 WO 2006/088949 PCT/US2006/005312 In one embodiment, Arl is substituted 1,2- fused aryl. In some aspects of the embodiment, Arl and the ring containing W 1 and W to which it is fused together form a substituted 3,4-dihydroisoquinolin-2(lH)-yl group, where 3,4-dihydroisoquinolin-2(lH)-yl group is shown in the following table in its unsubstituted form: Structure Name C I 3,4-dihydroisoquinolin-2(lH)-yl N 5 In one embodiment, W and W1 are optionally substituted methylene. In one embodiment, W is optionally substituted methylene and W 1 is optionally substituted ethylene. In one embodiment, W is optionally substituted ethylene and W1 is optionally substituted methylene. In one embodiment, W and W 1 are optionally substituted ethylene. 10 As used herein, "optionally substituted methylene" refers to -CRR 2 - and "optionally substituted ethylene" refers to -CR'R 2

CR'R

2 - where R' and R 2 are as defined above. In one preferred embodiment, W is methylene and W1 is ethylene. In another preferred embodiment, W is ethylene and W 1 is methylene. In another preferred embodiment, W and W1 are ethylene. 15 In one embodiment, W 2 is a bond. In one embodiment, W 2 is optionally substituted alkylene of the formula [-C(R)(R 2 )-]p. Preferably, W 2 either is a bond or is methylene. In one embodiment, Ar 2 is heteroarylene or substituted heteroarylene. Examples of -Ar2-G-C(O)NHOH include, for example, 5-[-G-C(O)NHOH]-pyrimid-2-ylene, 5-[-G C(O)NHOH]-thiazol-2-ylene, 5-[-G-C(O)NHOH]-pyrid-2-ylene, and 5-[-G-C(O)NHOH] 20 thien-2-ylene wherein attachment to -W2-Arl- is in all cases at the 2-position as shown in the following diagram. Structure Name 0 5-[-G-C(O)NHOH]-pyrimid-2-ylene N G4 N NHOH 6 WO 2006/088949 PCT/US2006/005312 G NHOH 5-[-G-C(O)NHOH]-thiazol-2-ylene 0 5-[-G-C(O)NHOH]-pyrid-2-ylene NHOH O 5-[-G-C(O)NHOH]-thien-2-ylene S G- NHOH In one embodiment, Ar 2 is arylene or substituted arylene. An example of an arylene group is 4-phenylene. Preferred -Ar 2 -G-C(O)NHOH groups include the 5-[-G-C(O)NHOH]-pyrimid-2 5 ylene and the 5-[-G-C(O)NHOH]-pyrid-2-ylene (and the N-oxide thereof). A particularly preferred Ar 2 group is 5-[-G-C(O)NHOH]-pyrimid-2-ylene. In one embodiment, G is a bond. In one embodiment, G is optionally substituted alkylene. In one embodiment, G is optionally substituted alkenylene; examples of which include, for instance, E-vinylene and Z-vinylene. In one embodiment, G is optionally 10 substituted alkynylene. In another of its compound aspects, this invention is directed to a compound of formula II: O aW N- G4 A N-W L NHOH /Z w x? II 15 wherein: 7 WO 2006/088949 PCT/US2006/005312 is Ar which is selected from the group consisting of substituted 1,2-fused aryl, 1,2 fused heteroaryl, substituted 1,2-fused heteroaryl, 1,2-fused heterocyclic, and substituted 1,2-fused heterocyclic; Arl is fused to the ring containing W1 and W; 5 W and W1 are independently [-C(R')(R 2 )-]m;

W

2 is a bond or [-C(Rig2

R

1 and R 2 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic; 10 each m is independently 1 or 2; pis 1, 2, 3, or4; X is CH or N; Z is absent or is selected from the group consisting of halo, alkyl, and substituted alkyl; and 15 G is selected from the group consisting of a bond, alkylene, substituted alkylene, alkenylene, substituted alkenylene, alkynylene, and substituted alkynylene, or a tautomer, stereoisomer, prodrug, or pharmaceutically acceptable salt thereof. In one embodiment, Ar' is 1,2-fused heteroaryl, substituted 1,2-fused heteroaryl, 1,2-fused heterocyclic, or substituted 1,2-fused heterocyclic. In some aspects of the 20 embodiment, Ar' and the ring containing W1 and W to which it is fused together form the optionally substituted groups in the following table: Structure Name H 1,3,4,9-tetrahydro-2H-b-carbolin-2-yl N NA H 1,3,4,4a,9,9a-hexahydro-2H-b-carbolin-2 N 8yl 8 WO 2006/088949 PCT/US2006/005312 1, 3,4,5ahvdro-2Hpyri43bdol 3 iNd2-yl N HN 1 a,4,65-tetahydro2Hepin[45ido4l 3N(211)-yl N H 13 ,4,-tethydro-2enoHeO, pyridbin S NI ~21-yl NA 1,4,5,6-tetrahydroazepino(4,5 -blindol 3(2H)-yl 3,4-dihydro[1]benzothieno[2,3-c]pyridin 2(1H)-y1 3,4-dihydro[1]benzofuro[2,3-c]pyridin 0 N 2(1H)-yl 0 10-oxo-3,4,5,10-tetrahydrobenzo[b]-1,6 : naphthyridin-2(lH)-yl. H In one embodiment, Ar is substituted 1,2-fused aryl. In some aspects of the embodiment, Ar' and the ring containing W1 and W to which it is fused together form a substituted 3,4-dihydroisoquinolin-2(lH)-yl group. In one embodiment, W and W1 are 5 optionally substituted methylene. In one embodiment, W is optionally substituted methylene and W 1 is optionally substituted ethylene. In one embodiment, W is optionally substituted ethylene and W 1 is optionally substituted methylene. In one embodiment, W and W1 are optionally substituted ethylene. As used herein, "optionally substituted methylene" refers to -CRR 2 - and "optionally substituted ethylene" refers to -CRR 2

CRR

2 10 where R 1 and R 2 are as defined above. In one preferred embodiment, W is methylene and W1 is ethylene. In another preferred embodiment, W is ethylene and W 1 is methylene. In 9 WO 2006/088949 PCT/US2006/005312 another preferred embodiment, W and W1 are ethylene. In one embodiment, W 2 is a bond. In one embodiment, W2 is optionally substituted alkylene of the formula [-C(R')(R 2 )-]p. Preferably, W 2 either is a bond or is methylene. In one embodiment, X is CH (i.e., pyridyl). Preferred pyridyl groups include 5-[-G 5 C(O)NHOH]-pyrid-2-yl and the N-oxide thereof. In another embodiment, X is nitrogen (i.e., pyrimidinyl). Particularly preferred pyrimidinyl groups include 5-[-G-C(O)NHOH] pyrimidin-2-yl. In one embodiment, Z is absent. In another embodiment, Z is halogen, alkyl or substituted alkyl (e.g., trifluoromethyl). 10 In one embodiment, G is a bond. In one embodiment, G is optionally substituted alkylene. In one embodiment, G is optionally substituted alkenylene; examples of which include E-vinylene and Z-vinylene. In one embodiment, G is optionally substituted alkynylene. In still another of its compound aspects, this invention is directed to a compound of 15 formula III: 3 2 0 N-W-Ar-G A' (CH 2 )g NHOH III wherein 20 is Ar 3 wherein Ar 3 is substituted 1,2-fused aryl, 1,2-fused heteroaryl, substituted 1,2 fused heteroaryl, 1,2-fused heterocyclic, and substituted 1,2-fused heterocyclic; Ar 3 is fused to the adjacent piperidinyl or homopiperidinyl ring;

W

3 is a bond or [-C(RI)2 g is 1 or 2; 25 R', R 2 , p, Ar 2 , and G are as defined above; 10 WO 2006/088949 PCT/US2006/005312 or a tautomer, stereoisomer, prodrug, or pharmaceutically acceptable salts thereof; provided that when Ar 2 is arylene or substituted arylene, then G is not alkenylene or substituted alkenylene. In one embodiment, the compound of formula III is a compound of formula IIIa 0 N- G N- N= NHOH AC'(CH2)g X 5 Z IIIa wherein A', g, W3 are defined above for III and X, Z, and G are as defined above for II. In one embodiment, Ar 3 is 1,2-fused heteroaryl, substituted 1,2-fused heteroaryl, 1,2-fused heterocyclic, or substituted 1,2-fused heterocyclic. Examples of such Ar 3 groups N AC'(CH2)g coupled to the piperidinyl or homopiperidinyl ring as represented by 10 include optionally substituted: Structure Name H 1,3,4,9-tetrahydro-2H-b-carbolin-2-yl N N H 1,3,4,4a,9,9a-hexahydro-2H-b-carbolin-2 N NI 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol 2-yl N H 1,1a,3,4,4a,5-hexahydro-2H-pyrido[4,3 b]indol2-yl N H 11 WO 2006/088949 PCT/US2006/005312 1,4,5,6-tetrahydroazepino[4,5-b]indol N 3(2H)-yl N H 3,4-dihydro[1]benzothieno[2,3-c]pyridin S N] 2(lH)-yl 3,4-dihydro [1 ]benzofuro [2,3 -c]pyridin 0 N 2(lH)-yl o 10-oxo-3,4,5,10-tetrahydrobenzo[b]-1,6 N naphthyridin-2(lH)-yl H In one embodiment, Ar 3 is substituted 1,2- fused aryl. In some aspects of the embodiment, Ar 3 is a substituted 3,4-dihydroisoquinolin-2(1H)-yl group. In one embodiment, W 3 is a bond. In one embodiment, W 3 is optionally substituted alkylene of the 5 formula [-C(R')(R 2 )-]p. Preferably, W 3 either is a bond or is methylene. In one embodiment, g is one. In another embodiment, g is two. In one embodiment, Ar 2 is heteroarylene or substituted heteroarylene. Examples of -Ar 2 -G-C(O)NHOH groups include, for example, 5-[-G-C(O)NHOH]-pyrimid-2-ylene, 5-[ G-C(O)NHOH]-thiazol-2-ylene, 5-[-G-C(O)NHOH]-pyrid-2-ylene, and 5-[-G 10 C(O)NHOH]thien-2-ylene wherein attachment to W 3 is in all cases at the 2-position. In one embodiment, Ar2 is arylene or substituted arylene. An example of an arylene group is 4-phenylene. Preferred -Ar 2 -G-C(O)NHOH groups include 5-[-G-C(O)NHOH]-pyrimid-2-ylene and 5-[-G-C(O)NHOH]-pyrid-2-ylene (and the N-oxide thereof). A particularly preferred 15 -Ar 2 -G-C(O)NHOH group is 5-[-G-C(O)NHOH]-pyrimid-2-ylene. In one embodiment, G is a bond. In one embodiment, G is optionally substituted 12 WO 2006/088949 PCT/US2006/005312 alkylene. In one embodiment, G is optionally substituted alkenylene; examples of which include E-vinylene and Z-vinylene. In one embodiment, G is optionally substituted alkynylene. In still another of its compound aspects, this invention is directed to a compound of 5 formula IV or a tautomer, stereoisomer, prodrug, or pharmaceutically acceptable salt thereof: - L WN-W 2 -Ar 2 -G T W NHOH IV wherein: 10 L is a bond or C(O), such that when L is a bond the central ring containing L is a five membered ring and when L is C(O) the central ring containing L is a six membered ring; the dashed line -- represents a single or double bond; T is 0, S, or -N-(Y)q-R3 15 Y is selected from the group consisting of -C(O)- and -S(0) 2 -; q is equal to 0 or 1; RW is selected from the group consisting of hydrogen, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic and substituted heterocyclic;

R

4 is selected from the group consisting of acyl, acylamino, alkyl, substituted alkyl, 20 alkoxy, substituted alkoxy, amino, substituted amino, aminoacyl, aminocarbonyloxy, oxycarbonylamino, aryl, substituted aryl, carboxyl, carboxyl ester, cyano, halo, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic, hydroxyl, nitro, thiol, thioalkyl, and substituted thioalkyl; n is 0, 1, 2, or 3; 25 W and W1 are independently [-C(R)(R 2 )-]m;

W

2 is a bond or [-C(R')(R27_ 13 WO 2006/088949 PCT/US2006/005312 R' and R 2 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic; each m is independently 1 or 2; 5 pis1,2,3 or4; Ar2 is selected from the group consisting of arylene, substituted arylene, heteroarylene, and substituted heteroarylene; and G is selected from the group consisting of a bond, alkylene, substituted alkylene, alkenylene, substituted alkenylene, alkynylene, and substituted alkynylene; 10 provided that when Ar 2 is arylene or substituted arylene, then G is not alkenylene or substituted alkenylene. In one embodiment, L is a bond. In one embodiment, T is 0 or S. In one embodiment, T is -N-(Y)q-R 3 . 15 In one embodiment, R3 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, and aryl. In other embodiments, q is zero and R3 is selected from the group consisting of hydrogen, methyl, (pyrrolidin- 1 -yl)methyl, (2-pyrrolidin- 1 -yl)eth- 1 -yl, (2-piperidin-1-yl)eth-1-yl, 2-(morpholin-4-yl)eth-1-yl, and benzyl. In another embodiment, q is zero and R3 is hydrogen. 20 In one embodiment, q is one, Y is -S(0) 2 -, and R3 is phenyl. In another embodiment, q is one, Y is -C(O)-, and -Y- R 3 together form an acyl group. In one embodiment, n is zero. In another embodiment, n is one or two. In one embodiment, R 4 is selected from the group consisting of alkyl, substituted 25 alkyl, alkoxy, substituted alkoxy, amino, substituted amino, nitro, heterocyclic, substituted heterocyclic, aryl, substituted aryl, heteroaryl, substituted heteroaryl, and halo. In another embodiment R 4 is selected from the group consisting of methyl, methoxy, bromo, chloro, fluoro, trifluoromethyl, trifluoromethoxy, phenyl, 4-(4-methylpiperazin- 1 14 WO 2006/088949 PCT/US2006/005312 ylmethyl)phenyl, 3-(4-methylpiperazin-1-ylmethyl)phenyl, 2-(4-methylpiperazin-1 ylmethyl)phenyl, 3-(morpholin-4-yl)methyl)phenyl, 4-(morpholin-4-yl)methyl)phenyl, 3 (pyrrolidin-1-ylmethyl)phenyl, 4-(pyrrolidin-1-ylmethyl)phenyl, 3-pyridyl, 5-pyrimidinyl, 4-acetamidophenyl, 3-(NN-dimethylaminomethyl)phenyl, 3-(piperidin-1-ylmethyl)phenyl, 5 3-(thiomorpholin-4-ylmethyl)phenyl, 4-(thiomorpholin-4-ylmethyl)phenyl, 3-(piperazin-1 ylmethyl)phenyl, 3-(4-methylsulfonylpiperazin-1-ylmethyl)phenyl, 3-(4-acetylpiperazin-1 ylmethyl)phenyl, 3-hydroxymethylphenyl, 4-methoxyphenyl, 4-methylphenyl, 3 (pyrrolidin-1-ylcarbonyl)phenyl, 4-hydroxymethylphenyl, 3-aminophenyl, 3-fluorophenyl, 4-fluorophenyl, 3-furyl, 3-(N-methanesulfonamidomethyl)phenyl, 3-(N 10 acetamidomethyl)phenyl, 3-methylthiophenyl, 3-methylsulfinylphenyl, 4-NN dimethylaminophenyl, 3-(1H-tetrazol-5-yl)phenyl, 5-(4-methylpiperazin-1-ylmethyl)-2 furyl, 5-(morpholin-4-ylmethyl)-2-furyl, 5-(4-methylpiperrazin-1-ylmethyl)-2-thienyl, 1H 2-pyrrolyl, 2-thienyl and the like In one embodiment, W and W 1 are optionally substituted methylene. In one 15 embodiment, W is optionally substituted methylene and W1 is optionally substituted ethylene. In one embodiment, W is optionally substituted ethylene and W1 is optionally substituted methylene. In one embodiment, W and W 1 are optionally substituted ethylene. As used herein, "optionally substituted methylene" refers to -CR 1

R

2 - and "optionally substituted ethylene" refers to -CR'R 2

CR'R

2 - where R1 and R 2 are as defined above. In one 20 preferred embodiment, W is methylene and W 1 is ethylene. In another preferred embodiment, W is ethylene and W 1 is methylene. In another preferred embodiment, W and W1 are ethylene. In one embodiment, W2 is a bond. In one embodiment, W2 is optionally substituted alkylene of the formula [-C(R)(R 2 )-]p. Preferably, W 2 either is a bond or is methylene. 25 In one embodiment, Ar 2 is heteroarylene or substituted heteroarylene. Examples of -Ar 2 -G-C(O)NHOH groups include, for example, 5-[-G-C(O)NHOH]-pyrimid-2-ylene, 5-[ G-C(O)NHOH]-thiazol-2-ylene, 5-[-G-C(O)NHOH]-pyrid-2-ylene, and 5-[-G C(O)NHOH]-thien-2-ylene wherein attachment to W 2 is in all cases at the 2-position as shown in the following diagram. 15 WO 2006/088949 PCT/US2006/005312 Structure Name G O 5-[-G-C(O)NHOH]-pyrimid-2-ylene NHOH N G NHOH 5-[-G-C(O)NHOH]-thiazol-2-ylene o 5-[-G-C(O)NHOH]-pyrid-2-ylene NHOH N O 5-[-G-C(O)NHOH]-thien-2-ylene S G- NHOH In one embodiment, Ar 2 is arylene or substituted arylene. An example of an arylene group is 4-phenylene. In one embodiment -Ar2-G-C(O)NHOH groups include 5-[-G-C(O)NHOH] 5 pyrimid-2-ylene and 5-[-G-C(O)NHOH]-pyrid-2-ylene (and the N-oxide thereof). In another embodiment -Ar 2 -G-C(O)NHOH group is 5-[-G-C(O)NHOH]-pyrimid-2-ylene. In one embodiment, G is a bond. In one embodiment, G is optionally substituted alkylene. In one embodiment, G is optionally substituted alkenylene; examples of which include, for instance, E-vinylene and Z-vinylene. In one embodiment, G is optionally 10 substituted alkynylene. In still another of its compound aspects, this invention is directed to a compound of formulae V(A) or V(B): 0 0 X G4 X G4 N-W NHOH N-W NHOH

(CH

2 )g N I (CH 2 )g NI N Z N z (R4)" (R4). (Y)q (Y)q

R

3 1 16 WO 2006/088949 PCT/US2006/005312 V(A) V(B) wherein W 2 , g, X, Z and G are as defined above;

R

3 is selected from the group consisting of hydrogen, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic and substituted heterocyclic; 5 R 4 is selected from the group consisting of acyl, acylamino, alkyl, substituted alkyl, alkoxy, substituted alkoxy, amino, substituted amino, aminoacyl, aminocarbonyloxy, oxycarbonylamino, aryl, substituted aryl, carboxyl, carboxyl ester, cyano, halo, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic, hydroxyl, nitro, thiol, thioalkyl and substituted thioalkyl; 10 Y is selected from the group consisting of -C(O)- and -S(O) 2 -; n is equal to 0, 1, 2 or 3; q is equal to 0 or 1; or a stereoisomer, tautomer, prodrug, or pharmaceutically acceptable salt thereof. In one embodiment, when q is other than zero, Y is carbonyl. In another 15 embodiment, when q is other than zero, Y is -SO 2 -. In still another embodiment, q is zero. In one embodiment, g is one. In another embodiment, g is two. In one embodiment, R 3 is selected from the group consisting of hydrogen, alkyl, substituted alkyl and aryl. Examples of R 3 when q is zero include hydrogen, methyl, (pyrrolidin-1-yl)methyl, (2-pyrrolidin-1-yl)eth-1-yl, (2-piperidin-1-yl)eth-1-yl, 2 20 (morpholin-4-yl)eth-1-yl, benzyl, and the like. Preferably, R 3 is hydrogen. Examples of R Y- when q is one include phenylsulfonyl and acyl. In one embodiment, n is zero. In another embodiment, n is one, two or three, and is preferably one or two. When n is other than zero, R 4 is preferably selected from the group consisting of 25. alkyl, substituted alkyl, alkoxy, substituted alkoxy, heterocyclic, substituted heterocyclic, aryl, substituted aryl, heteroaryl, substituted heteroaryl and halo. Particularly preferred R 4 groups include methyl, methoxy, bromo, chloro, fluoro, trifluoromethyl, trifluoromethoxy, phenyl, 4-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(4-methylpiperazin-1-ylmethyl)phenyl, 2-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(morpholin-4-yl)methyl)phenyl, 4-(morpholin 30 4-yl)methyl)phenyl, 3-(pyrrolidin-1-ylmethyl)phenyl, 4-(pyrrolidin-1-ylmethyl)phenyl, 3 17 WO 2006/088949 PCT/US2006/005312 pyridyl, 5-pyrimidinyl, 4-acetamidophenyl, 3-(NN-dimethylaminomethyl)phenyl, 3 (piperidin-1-ylmethyl)phenyl, 3-(thiomorpholin-4-ylmethyl)phenyl, 4-(thiomorpholin-4 ylmethyl)phenyl, 3-(piperazin-1-ylmethyl)phenyl, 3-(4-methylsulfonylpiperazin-1 ylmethyl)phenyl, 3-(4-acetylpiperazin-1-ylmethyl)phenyl, 3-hydroxymethylphenyl, 4 5 methoxyphenyl, 4-methylphenyl, 3-(pyrrolidin-1-ylcarbonyl)phenyl, 4 hydroxymethylphenyl, 3-aminophenyl, 3-fluorophenyl, 4-fluorophenyl, 3-furyl, 3-(N methanesulfonamidomethyl)phenyl, 3-(N-acetamidomethyl)phenyl, 3-methylthiophenyl, 3 methylsulfinylphenyl, 4-NN-dimethylaminophenyl, 3-(1H-tetrazol-5-yl)phenyl, 5-(4 methylpiperazin-1-ylmethyl)-2-furyl, 5-(morpholin-4-ylmethyl)-2-furyl, 5-(4 10 methylpiperrazin-1-ylmethyl)-2-thienyl, 1H-2-pyrrolyl, 2-thienyl and the likeln one embodiment, W 2 is a bond. In one embodiment, W 2 is optionally substituted alkylene of the formula [-C(R)(R 2 )-]p. Preferably, W 2 either is a bond or is methylene. In one embodiment, X is CH (i.e., pyridyl). Preferred pyridyl groups include the 5 [-G-C(O)NHOH]-pyrid-2-yl and the N-oxide thereof. In another embodiment, X is nitrogen 15 (i.e., pyrimidinyl). Particularly preferred pyrimidinyl groups include 5-[-G-C(O)NHOH] pyrimidin-2-yl. In one embodiment, Z is absent. In another embodiment, Z is halogen, alkyl or substituted alkyl (e.g., trifluoromethyl). In one embodiment, G is a bond. In one embodiment, G is optionally substituted 20 alkylene. In one embodiment, G is optionally substituted alkenylene; examples of which include E-vinylene and Z-vinylene. In one embodiment, G is optionally substituted alkynylene. In still another of its compound aspects, this invention is directed to a compound of formula VI(A) and VI(B) or a stereoisomer, tautomer, prodrug, or pharmaceutically 25 acceptable salt thereof: 18 WO 2006/088949 PCT/US2006/005312

R

3

R

3 (Y)q (Y)q N 0 N N X- G4 - X G W -\HOH Nw 'W K/ NHOH (R4)n (R4)n VI(A) VI(B) wherein R 3 , R 4 , W 2 , X, Y and G are as defined above. In one embodiment, when q is other than zero, Y is carbonyl. In another 5 embodiment, when q is other than zero, Y is -SO 2 -. In still another embodiment, q is zero. In one embodiment, R 3 is selected from the group consisting of hydrogen, alkyl, substituted alkyl and aryl. Examples of R 3 when q is zero include hydrogen, methyl, (pyrrolidin-1-yl)methyl, (2-pyrrolidin-1-yl)eth-1-yl, (2-piperidin-1-yl)eth-1-yl, 2 (morpholin-4-yl)eth-1-yl, benzyl, and the like. Preferably,

R

3 is hydrogen. Examples of R 3 10 Y- when q is one include phenylsulfonyl and acyl. In one embodiment, n is zero. In another embodiment, n is one, two or three, and is preferably one or two. When n is other than zero, R 4 is preferably selected from the group consisting of alkyl, substituted alkyl, alkoxy, substituted alkoxy, heterocyclic, substituted heterocyclic, 15 aryl, substituted aryl, heteroaryl, substituted heteroaryl and halo. Particularly preferred R 4 groups include methyl, methoxy, bromo, chloro, fluoro, trifluoromethyl, trifluoromethoxy, phenyl, 4-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(4-methylpiperazin-1-ylmethyl)phenyl, 2-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(morpholin-4-yl)methyl)phenyl, 4-(morpholin 4-yl)methyl)phenyl, 3-(pyrrolidin-1-ylmethyl)phenyl, 4-(pyrrolidin-1-ylmethyl)phenyl, 3 20 pyridyl, 5-pyrimidinyl, 4-acetamidophenyl, 3-(NN-dimethylaminomethyl)phenyl, 3 (piperidin-1-ylmethyl)phenyl, 3-(thiomorpholin-4-ylmethyl)phenyl, 4-(thiomorpholin-4 ylmethyl)phenyl, 3-(piperazin-1-ylmethyl)phenyl, 3-(4-methylsulfonylpiperazin-1 ylmethyl)phenyl, 3-(4-acetylpiperazin-1-ylmethyl)phenyl, 3-hydroxymethylphenyl, 4 methoxyphenyl, 4-methylphenyl, 3-(pyrrolidin-1-ylcarbonyl)phenyl, 4 25 hydroxymethylphenyl, 3-aminophenyl, 3-fluorophenyl, 4-fluorophenyl, 3-furyl, 3-(N methanesulfonamidomethyl)phenyl, 3-(N-acetamidomethyl)phenyl, 3-methylthiophenyl, 3 19 WO 2006/088949 PCT/US2006/005312 methylsulfinylphenyl, 4-NN-dimethylaminophenyl, 3-(lH-tetrazol-5-yl)phenyl, 5-(4 methylpiperazin-1-ylmethyl)-2-furyl, 5-(morpholin-4-ylmethyl)-2-furyl, 5-(4 methylpiperrazin-1-ylmethyl)-2-thienyl, 1H-2-pyrrolyl, 2-thienyl and the like. In one embodiment, W 2 is a bond. In one embodiment, W 2 is optionally substituted 5 alkylene of the formula [-C(R)(R 2 )-]p. Preferably, W 2 either is a bond or is methylene. In one embodiment, X is CH (i.e., pyridyl). Preferred pyridyl groups include the 5 [-G-C(O)NHOH]-pyrid-2-yl and the N-oxide thereof. In another embodiment, X is nitrogen (i.e., pyrimidinyl). Particularly preferred pyrimidinyl groups include 5-[-G-C(O)NHOH] pyrimidin-2-yl. 10 In one embodiment, Z is absent. In another embodiment, Z is halogen, alkyl or substituted alkyl (e.g., trifluoromethyl). In one embodiment, G is a bond. In one embodiment, G is optionally substituted alkylene. In one embodiment, G is optionally substituted alkenylene; examples of which include E-vinylene and Z-vinylene. In one embodiment, G is optionally substituted 15 alkynylene. In still another of its compound aspects, this invention is directed to a compound of formula VII or a stereoisomer, tautomer, prodrug, or pharmaceutically acceptable salt thereof: wherein the dashed line ----, W, W', R 3 , R 4 , Y, n and q are as defined above. In still another of its compound aspects, this invention is directed to a compound of 20 WO 2006/088949 PCT/US2006/005312 formula VII(A) and VII(B) or a stereoisomer, tautomer, prodrug, or pharmaceutical acceptable salt thereof: N- 0 N- 0 N / / NHOH (C2) N NH-OH N (CH2)g N I

CH

2 )gN'2 N (C

(R

4 )n (R4 VII(A) VII(B) 5 wherein RW, R 4 , Y, g, n and q are as defined above. In one embodiment, when q is other than zero, Y is carbonyl. In another embodiment, when q is other than zero, Y is -SO 2 -. In still another embodiment, q is zero. In one embodiment,

R

3 is selected from the group consisting of hydrogen, alkyl, substituted alkyl and aryl. Examples of R 3 when q is zero include hydrogen, methyl, 10 (pyrrolidin-1-yl)methyl, (2-pyrrolidin-1-yl)eth-1-yl, (2-piperidin-1-yl)eth-1-yl, 2 (morpholin-4-yl)eth-1-y, benzyl, and the like. Preferably,

R

3 is hydrogen. Examples of R Y- when q is one include phenylsulfonyl and acyl. In one embodiment, n is zero. In another embodiment, n is one, two or three, and is preferably one or two. 15 When n is other than zero, R 4 is preferably selected from the group consisting of alkyl, substituted alkyl, alkoxy, substituted alkoxy, heterocyclic, substituted heterocyclic, aryl, substituted aryl, heteroaryl, substituted heteroaryl and halo. Particularly preferred R groups include methyl, methoxy, bromo, chloro, fluoro, trifluoromethyl, trifluoromethoxy, phenyl, 4-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(4-methylpiperazin-1-ylmethyl)phenyl, 20 2-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(morpholin-4-yl)methyl)phenyl, 4-(morpholin 4-yl)methyl)phenyl, 3-(pyrrolidin-1-ylmethyl)phenyl, 4-(pyrrolidin-1-ylmethyl)phenyl, 3 pyridyl, 5-pyrimidinyl, 4-acetamidophenyl, 3 -(N,N-dimethylaminomethyl)phenyl, 3 (piperidin-1-ylmethyl)phenyl, 3 -(thiomorpholin-4-ylmethyl)phenyl, 4-(thiomorpholin- 4 ylmethyl)phenyl, 3-(piperazin-1-ylmethyl)phenyl, 3 -(4-methylsulfonylpiperazin-1 25 ylmethyl)phenyl, 3-(4-acetylpiperazin-1-ylmethyl)phenyl, 3-hydroxymethylphenyl, 4 methoxyphenyl, 4-methylphenyl, 3-(pyrrolidin-1-ylcarbonyl)phenyl, 4 21 WO 2006/088949 PCT/US2006/005312 hydroxymethylphenyl, 3-aminophenyl, 3-fluorophenyl, 4-fluorophenyl, 3-furyl, 3-(N methanesulfonamidomethyl)phenyl, 3-(N-acetamidomethyl)phenyl, 3-methylthiophenyl, 3 methylsulfinylphenyl, 4-NN-dimethylaminophenyl, 3-(lH-tetrazol-5-yl)phenyl, 5-(4 methylpiperazin-1-ylmethyl)-2-furyl, 5-(morpholin-4-ylmethyl)-2-furyl, 5-(4 5 methylpiperrazin-1-ylmethyl)-2-thienyl, 1H-2-pyrrolyl, 2-thienyl and the likeIn one embodiment g is one. In another embodiment g is two. In still another of its compound aspects, this invention is directed to a compound of formula VIII(A) and VIII(B) or a stereoisomer, tautomer, prodrug, or pharmaceutically acceptable salt thereof: 0 O NHOH NHOH NN N -N NN N (Y)

(R

4 )n (Y)q \3 10 VIII(A) VIII(B) wherein R 3 , R 4 , Y, n and q are as defined above. In one embodiment, when q is other than zero, Y is carbonyl. In another embodiment, when q is other than zero, Y is -SO 2 -. In still another embodiment, q is zero. 15 In one embodiment, R 3 is selected from the group consisting of hydrogen, alkyl, substituted alkyl and aryl. Examples of R3 when q is zero include hydrogen, methyl, (pyrrolidin-1-yl)methyl, (2-pyrrolidin-1-yl)eth-1-yl, (2-piperidin-1-yl)eth-1-yl, 2 (morpholin-4-yl)eth-1-yl, benzyl, and the like. Preferably, R3 is hydrogen. Example of R 3 _ Y- when q is one include phenylsulfonyl and acyl. 20 In one embodiment, n is zero. In another embodiment, n is one, two or three, and is 22 WO 2006/088949 PCT/US2006/005312 preferably one or two. When n is other than zero, R 4 is preferably selected from the group consisting of alkyl, substituted alkyl, alkoxy, substituted alkoxy, heterocyclic, substituted heterocyclic, aryl, substituted aryl, heteroaryl, substituted heteroaryl and halo. Particularly preferred R 4 5 groups include methyl, methoxy, bromo, chloro, fluoro, trifluoromethyl, trifluoromethoxy, phenyl, 4-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(4-methylpiperazin-1-ylmethyl)phenyl, 2-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(morpholin-4-yl)methyl)phenyl, 4-(morpholin 4-yl)methyl)phenyl, 3-(pyrrolidin-1-ylmethyl)phenyl, 4-(pyrrolidin-1-ylmethyl)phenyl, 3 pyridyl, 5-pyrimidinyl, 4-acetamidophenyl, 3-(NN-dimethylaminomethyl)phenyl, 3 10 (piperidin-1-ylmethyl)phenyl, 3-(thiomorpholin-4-ylmethyl)phenyl, 4-(thiomorpholin-4 ylmethyl)phenyl, 3-(piperazin-1-ylmethyl)phenyl, 3-(4-methylsulfonylpiperazin-1 ylmethyl)phenyl, 3-(4-acetylpiperazin-1-ylmethyl)phenyl, 3-hydroxymethylphenyl, 4 methoxyphenyl, 4-methylphenyl, 3-(pyrrolidin-1-ylcarbonyl)phenyl, 4 hydroxymethylphenyl, 3-aminophenyl, 3-fluorophenyl, 4-fluorophenyl, 3-furyl, 3-(N 15 methanesulfonamidomethyl)phenyl, 3-(N-acetamidomethyl)phenyl, 3-methylthiophenyl, 3 methylsulfinylphenyl, 4-NN-dimethylaminophenyl, 3-(1H-tetrazol-5-yl)phenyl, 5-(4 methylpiperazin-1-ylmethyl)-2-furyl, 5-(morpholin-4-ylmethyl)-2-furyl, 5-(4 methylpiperrazin- 1 -ylmethyl)-2-thienyl, 1H-2-pyrrolyl, 2-thienyl and the like. In still another of its compound aspects, this invention is directed to a compound of 20 formulae IX(A) or IX(B) or a stereoisomer, tautomer, prodrug, or pharmaceutically acceptable salt thereof: 0 2 X G4 2X G4 N-W NHOH N-W \ NHOH /N / H ~ (CH2)g N-1 (C2)g N -- CZ Z IX(A) IX(B) wherein W 2 , g, X, Z and G are as defined above; 25 Q is O or S; R4 is selected from the group consisting of acyl, acylamino, alkyl, substituted alkyl, alkoxy, substituted alkoxy, amino, substituted amino, aminoacyl, aminocarbonyloxy, 23 WO 2006/088949 PCT/US2006/005312 oxycarbonylamino, aryl, substituted aryl, carboxyl, carboxyl ester, cyano, halo, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic, hydroxyl, nitro, thiol, thioalkyl and substituted thioalkyl n is equal to 0, 1, 2 or 3. 5 In one embodiment, g is one. In another embodiment, g is two. In one embodiment, Q is oxygen. In another embodiment, Q is sulfur. In one embodiment, n is zero. In another embodiment, n is one, two or three, and is preferably one or two. When n is other than zero, R 4 is preferably selected from the group consisting of 10 alkyl, substituted alkyl, alkoxy, substituted alkoxy, heterocyclic, substituted heterocyclic, aryl, substituted aryl, heteroaryl, substituted heteroaryl and halo. Particularly preferred R4 groups include methyl, methoxy, bromo, chloro, fluoro, trifluoromethyl, trifluoromethoxy, phenyl, 4-(4-methylpiperazin- 1 -ylmethyl)phenyl, 3 -(4-methylpiperazin- 1 -ylmethyl)phenyl, 2-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(morpholin-4-yl)methyl)phenyl and the like. 15 In one embodiment, W2 is a bond. In one embodiment, W2 is optionally substituted alkylene of the formula [-C(R )(R2)-]p. Preferably, W 2 either is a bond or is methylene. In one embodiment, X is carbon (i.e., pyridyl). Preferred pyridyl groups include the 5-[-G-C(O)NHOH]-pyrid-2-yl and the N-oxide thereof. In another embodiment, X is nitrogen (i.e., pyrimidinyl). Particularly preferred pyrimidinyl groups include 5-[-G 20 C(O)NHOH]-pyrimidin-2-yl. In one embodiment, Z is absent. In another embodiment, Z is halogen, alkyl or substituted alkyl (e.g., trifluoromethyl). In one embodiment, G is a bond. In one embodiment, G is optionally substituted alkylene. In one embodiment, G is optionally substituted alkenylene; examples of which 25 include E-vinylene and Z-vinylene. In one embodiment, G is optionally substituted alkynylene. In still another of its compound aspects, this invention is directed to a compound of formula X(A) and X(B) or a stereoisomer, tautomer, prodrug, or pharmaceutically 24 WO 2006/088949 PCT/US2006/005312 acceptable salt thereof: N- 0 N- 0 N NHOH HC N/-NHOH \ (C 1

H

2

)

9 N NHH(CH 2

)

9 NHH /Q (R4)n (R4 X(A) X(B) wherein R 4 , Q, g, and n are as defined above. 5 In one embodiment, n is zero. In another embodiment, n is one, two or three, and is preferably one or two. When n is other than zero, R 4 is preferably selected from the group consisting of alkyl, substituted alkyl, alkoxy, substituted alkoxy, heterocyclic, substituted heterocyclic, aryl, substituted aryl, heteroaryl, substituted heteroaryl and halo. Particularly preferred R 4 10 groups include methyl, methoxy, bromo, chloro, fluoro, trifluoromethyl, trifluoromethoxy, phenyl, 4-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(4-methylpiperazin-1-ylmethyl)phenyl, 2-(4-methylpiperazin- 1 -ylmethyl)phenyl, 3 -(morpholin-4-yl)methyl)phenyl and the like. In one embodiment g is one. In another embodiment g is two. In one embodiment, Q is oxygen. In another embodiment, Q is sulfur. 15 In still another of its compound aspects, this invention is directed to a compound of formula XI(A) and XI(B) or a stereoisomer, tautomer, prodrug, or pharmaceutically acceptable salt thereof: R3 R3 I I (Y)q (Y)q N N 0 0~ 4N 2 G NHOH N NHOH R n N-1) 0 N-1 Z Z 20 XI(A) XI(B) wherein R3, R 4 , W 2 , X, Y, Z, G, n, and q are as defined above. 25 WO 2006/088949 PCT/US2006/005312 In one embodiment, when q is other than zero, Y is carbonyl. In another embodiment, when q is other than zero, Y is -SO 2 -. In still another embodiment, q is zero. In one embodiment, R3 is selected from the group consisting of hydrogen, alkyl, substituted alkyl and aryl. Examples of R3 when q is zero include hydrogen, methyl, 5 (pyrrolidin-1-yl)methyl, (2-pyrrolidin-1-yl)eth-1-yl, (2-piperidin-1-yl)eth-1-yl, 2 (morpholin-4-yl)eth-1-yl, benzyl, and the like. Preferably, R 3 is hydrogen. Examples of R3 Y- when q is one include phenylsulfonyl and acyl. In one embodiment, n is zero. In another embodiment, n is one, two or three, and is preferably one or two. 10 When n is other than zero, R 4 is preferably selected from the group consisting of alkyl, substituted alkyl, alkoxy, substituted alkoxy, heterocyclic, substituted heterocyclic, aryl, substituted aryl, heteroaryl, substituted heteroaryl and halo. Particularly preferred R 4 groups include methyl, methoxy, bromo, chloro, fluoro, trifluoromethyl, trifluoromethoxy, phenyl, 4-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(4-methylpiperazin-1-ylmethyl)phenyl, 15 2-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(morpholin-4-yl)methyl)phenyl, 4-(morpholin 4-yl)methyl)phenyl, 3-(pyrrolidin-1-ylmethyl)phenyl, 4-(pyrrolidin-1-ylmethyl)phenyl, 3 pyridyl, 5-pyrimidinyl, 4-acetamidophenyl, 3-(NN-dimethylaminomethyl)phenyl, 3 (piperidin-1-ylmethyl)phenyl, 3-(thiomorpholin-4-ylmethyl)phenyl, 4-(thiomorpholin-4 ylmethyl)phenyl, 3-(piperazin-1-ylmethyl)phenyl, 3-(4-methylsulfonylpiperazin-1 20 ylmethyl)phenyl, 3-(4-acetylpiperazin-1-ylmethyl)phenyl, 3-hydroxymethylphenyl, 4 methoxyphenyl, 4-methylphenyl, 3-(pyrrolidin-1-ylcarbonyl)phenyl, 4 hydroxymethylphenyl, 3-aminophenyl, 3-fluorophenyl, 4-fluorophenyl, 3-furyl, 3-(N methanesulfonamidomethyl)phenyl, 3-(N-acetamidomethyl)phenyl, 3-methylthiophenyl, 3 methylsulfinylphenyl, 4-NN-dimethylaminophenyl, 3-(1H-tetrazol-5-yl)phenyl, 5-(4 25 methylpiperazin-1-ylmethyl)-2-furyl, 5-(morpholin-4-ylmethyl)-2-furyl, 5-(4 methylpiperrazin-1 -ylmethyl)-2-thienyl, 1H-2-pyrrolyl, 2-thienyl and the like. In one embodiment, W 2 is a bond. In one embodiment, W 2 is optionally substituted alkylene of the formula [-C(R')(R 2 )-]p. Preferably, W 2 either is a bond or is methylene. In one embodiment, X is carbon (i.e., pyridyl). Preferred pyridyl groups include the 30 5-[-G-C(O)NHOH]-pyrid-2-yl and the N-oxide thereof. In another embodiment, X is 26 WO 2006/088949 PCT/US2006/005312 nitrogen (i.e., pyrimidinyl). Particularly preferred pyrimidinyl groups include 5-[-G C(O)NHOH]-pyrimidin-2-yl. In one embodiment, Z is absent. In another embodiment, Z is halogen, alkyl or substituted alkyl (e.g., trifluoromethyl). 5 In one embodiment, G is a bond. In one embodiment, G is optionally substituted alkylene. In one embodiment, G is optionally substituted alkenylene; examples of which include E-vinylene and Z-vinylene. In one embodiment, G is optionally substituted alkynylene. In still another of its compound aspects, this invention is directed to a compound of 10 formula XII(A) and XII(B) or a stereoisomer, tautomer, prodrug, or pharmaceutically acceptable salt thereof: R3 R3 I I (Y)q (Y)q N N NI N N N (R4)n 0 (R4 )n O N0 N : -- 0 NHOH NHOH XII(A) XII(B) wherein R 3 , R 4 , Y, n and q are as defined above. 15 In one embodiment, when q is other than zero, Y is carbonyl. In another embodiment, when q is other than zero, Y is -SO 2 -. In still another embodiment, q is zero. In one embodiment, R 3 is selected from the group consisting of hydrogen, alkyl, substituted alkyl and aryl. Examples of R 3 when q is zero include hydrogen, methyl, (pyrrolidin- 1 -yl)methyl, (2-pyrrolidin- 1 -yl)eth- 1 -yl, (2-piperidin- 1 -yl)eth- 1 -yl, 2 20 (morpholin-4-yl)eth-1-yl, benzyl, and the like. Preferably, R 3 is hydrogen. Example of R Y- when q is one include phenylsulfonyl and acyl. In one embodiment, n is zero. In another embodiment, n is one, two or three, and is preferably one or two. 27 WO 2006/088949 PCT/US2006/005312 When n is other than zero, R 4 is preferably selected from the group consisting of alkyl, substituted alkyl, alkoxy, substituted alkoxy, heterocyclic, substituted heterocyclic, aryl, substituted aryl, heteroaryl, substituted heteroaryl and halo. Particularly preferred R 4 groups include methyl, methoxy, bromo, chloro, fluoro, trifluoromethyl, trifluoromethoxy, 5 phenyl, 4-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(4-methylpiperazin-1-ylmethyl)phenyl, 2-(4-methylpiperazin-1-ylmethyl)phenyl, 3-(morpholin-4-yl)methyl)phenyl, 4-(morpholin 4-yl)methyl)phenyl, 3-(pyrrolidin-1-ylmethyl)phenyl, 4-(pyrrolidin-1-ylmethyl)phenyl, 3 pyridyl, 5-pyrimidinyl, 4-acetamidophenyl, 3-(NN-dimethylaminomethyl)phenyl, 3 (piperidin-1-ylmethyl)phenyl, 3-(thiomorpholin-4-ylmethyl)phenyl, 4-(thiomorpholin-4 10 ylmethyl)phenyl, 3-(piperazin-1-ylmethyl)phenyl, 3-(4-methylsulfonylpiperazin-1 ylmethyl)phenyl, 3-(4-acetylpiperazin-1-ylmethyl)phenyl, 3-hydroxymethylphenyl, 4 methoxyphenyl, 4-methylphenyl, 3-(pyrrolidin-1-ylcarbonyl)phenyl, 4 hydroxymethylphenyl, 3-aminophenyl, 3-fluorophenyl, 4-fluorophenyl, 3-furyl, 3-(N methanesulfonamidomethyl)phenyl, 3-(N-acetamidomethyl)phenyl, 3-methylthiophenyl, 3 15 methylsulfinylphenyl, 4-NN-dimethylaminophenyl, 3-(1H-tetrazol-5-yl)phenyl, 5-(4 methylpiperazin-1-ylmethyl)-2-furyl, 5-(morpholin-4-ylmethyl)-2-furyl, 5-(4 methylpiperrazin-1 -ylmethyl)-2-thienyl, 1H-2-pyrrolyl, 2-thienyl and the like. In one of its pharmaceutical composition aspect, this invention is directed to a pharmaceutical composition comprising an effective amount of one or more compounds 20 according to formula I, II, III, III(A), IV, V(A), V(B) VI(A), VI(B), VII, VII(A), VII(B), VIII(A), VIII(B), IX(A), IX(B), X(A), X(B), XI(A), XI(B), XII(A) and/or XII(B) and a pharmaceutically inert carrier. In another of its pharmaceutical aspects, this invention is directed to pharmaceutical compositions comprising an effective amount of one or more compounds according to 25 formula I, II, III, III(A), IV, V(A), V(B) VI(A), VI(B), VII, VII(A), VII(B), VIII(A),VIII(B), IX(A), IX(B), X(A), X(B), XI(A), XI(B), XII(A) and/or XII(B) and an effective amount of at least one anti-cancer agent, and a pharmaceutically inert carrier. In another of its pharmaceutical aspects, this invention is directed to use of a compound according to formula I, II, III, III(A), IV, V(A), V(B) VI(A), VI(B), VII, VII(A), 30 VII(B), VIII(A),VIII(B), IX(A), IX(B), X(A), X(B), XI(A), XI(B), XII(A) and/or XII(B) for 28 WO 2006/088949 PCT/US2006/005312 the manufacture of a medicament for treating the conditions disclosed herein. Deacetylases are found in transcriptional repression pathways. In addition, histone deacetylases (HDAC) play an important role in cell proliferation and differentiation. Inhibition of histone deacetylation results in cell cycle arrest, cellular differentiation, 5 apoptosis and reversal of the transformed phenotype. Therefore, HDAC inhibitors are useful in the treatment and/or amelioration of cell proliferative diseases or conditions, such as cancers. Other diseases in which said HDAC inhibitors are useful are hematological disorders, e.g., hemoglobinopathies (thalassemias, sickle cell anemias); autosomal dominant disorders, e.g., spinal muscular atrophy and Huntington's disease; genetic related metabolic 10 disorder, e.g., cystic fibrosis and adrenoleukodystrophy (US2004/0029903 Al, US 6,124,495); psoriasis (McLaughlin, F.; La Thangue, N. B., Current Drug Targets Inflammation, 2004, 3, 213-219); fibrosis, e.g., liver fibrosis, cirrhosis and fibrotic skin diseases, e.g., hypertrophic scars, keloid and Dupuytren's contracture (US 5,993,845); autoimmune diseases, e.g., systemic lupus erythematosus (US2003/0082666 Al); acute or 15 chronic degenerative conditions or diseases of the eye, e.g., glaucoma, dry age-related macular degeneration, retinitis pigmentosa and other forms of heredodegenerative retinal disease, retinal detachment and tears; macular pucker, ischemia affecting the outer retina, cellular damage associated with diabetic retinopathy and retinal ischemia, damage associated with laser therapy (grid, focal, and panretinal) including photodynamic therapy, 20 trauma, surgical (retinal translocation, subretinal surgery, or vitrectomy) or light-induced iatrogenic retinopathy, and preservation of retinal transplants (US2004/0092431 Al); ocular neovascular or edematous diseases and disorders, e.g., diabetic retinopathy, rubeosis iritis, uveitis, Fuch's heterochromatic iridocyclitis, neovascular glaucoma, corneal neovascularization, neovasculariztion resulting from combined vitrectomy and lensectomy, 25 retinal ischemia, choroidal vascular insufficiency, choroidal thrombosis, carotid artery ischemia, contusive ocular injury, retinopathy of prematurity, retinal vein occlusion, proliferative vitreoretinopathy, corneal angiogenesis, retinal microvasculopathy, or retinal edema (US 2004/0092558 Al); connective tissue disease, e.g., rheumatoid arthritis, progressive systemic sclerosis, sjorgren's syndrome, dermatomyositis or mixed connective 30 tissue disease (US 2003/0206946 Al); cardiac hypertrophy and heart failure (US 6,706,686 B2); insulin resistance (US 2004/0058868 Al); amyotrophic lateral sclerosis (US 29 WO 2006/088949 PCT/US2006/005312 2004/0077591 Al); multiple sclerosis (US 2004/0077591 Al); Alzheimer's disease (US 2004/0077591 Al); neurodegenerative diseases (US 2004/0087657 Al); and lung diseases, e.g., cystic fibrosis, chronic obstructive pulmonary disease, asthma or acute and chronic bronchitis (US 2004/0167184 Al). Each of the above references is incorporated herein by 5 reference in their entirety. In one of its method aspects, this invention is directed to a method for inhibiting a proliferative disorder in a mammalian patient which method comprises administering to said patient a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a therapeutically effective amount of one or more compounds of formula I, II, III, III(A), 10 IV, V(A), V(B) VI(A), VI(B), VII, VII(A), VII(B), VIII(A),VIII(B), IX(A), IX(B), X(A), X(B), XI(A), XI(B), XII(A) and/or XII(B). In another of its method aspects, this invention is directed to a method for inhibiting a proliferative disorder in a mammalian patient which method comprises administering to said patient a phannaceutical composition comprising a pharmaceutically acceptable carrier, an effective amount of at least one anti-cancer agent, 15 and a therapeutically effective amount of one or more compounds of formula I, II, III, III(A), IV, V(A), V(B) VI(A), VI(B), VII, VII(A), VII(B), VIII(A),VIII(B), IX(A), IX(B), X(A), X(B), XI(A), XI(B), XII(A) and/or XII(B). In yet another of its method aspects, this invention is directed to a method for inhibiting a proliferative disorder in a mammalian patient which method comprises administering to said patient a pharmaceutical composition 20 comprising a pharmaceutically acceptable carrier and a therapeutically effective amount of one or more compounds of formula I, II, III, III(A), IV, V(A), V(B) VI(A), VI(B), VII, VII(A), VII(B), VIII(A),VIII(B), IX(A), IX(B), X(A), X(B), XI(A), XI(B), XII(A) and/or XII(B) in combination with at least one anti-cancer agent. For the treatment of the above conditions, the compounds of the invention may be 25 advantageously employed in combination with one or more other medicinal agents, more particularly, with other anti-cancer agents. Examples of anti-cancer agents are: platinum coordination compounds, for example, cisplatin, carboplatin or oxalyplatin; taxane compounds, for example, paclitaxel or docetaxel; topoisomerase I inhibitors such as camptothecin compounds, for example, irinotecan or topotecan; topoisomerase II inhibitors 30 such as anti-tumour podophyllotoxin derivatives, for example, etoposide or teniposide; anti 30 WO 2006/088949 PCT/US2006/005312 tumour vinca alkaloids, for example, vinblastine, vincristine or vinorelbine; anti-tumor nucleoside derivatives, for example, 5-fluorouracil, gemcitabine or capecitabine; alkylating agents such as nitrogen mustard or nitrosourea, for example, cyclophosphamide, chlorambucil, carmustine or lomustine; anti-tumour anthracycline derivatives, for example, 5 daunorubicin, doxorubicin, idarubicin or mitoxantrone; HER2 antibodies, for example, trastuzumab; estrogen receptor antagonists or selective estrogen receptor modulators, for example, tamoxifen, toremifene, droloxifene, faslodex or raloxifene; aromatase inhibitors such as exemestane, anastrozole, letrazole and vorozole; differentiating agents such as retinoids, vitamin D and retinoic acid metabolism blocking agents (RAMBA), for example, 10 accutane; DNA methyl transferase inhibitors, for example, azacytidine; kinase inhibitors, for example, flavoperidol, imatinib mesylate or gefitinib; farnesyltransferase inhibitors; or other HDAC inhibitors. In another of its method aspects, this invention is directed to a method for treating a mammalian patient with one or more diseases or disorders including hematological 15 disorders, e.g., hemoglobinopathies (thalassemias, sickle cell anemias); autosomal dominant disorders, e.g., spinal muscular atrophy and Huntington's disease; genetic related metabolic disorders, e.g., cystic fibrosis and adrenoleukodystrophy; psoriasis; fibrosis, e.g., liver fibrosis, cirrhosis and fibrotic skin diseases, e.g., hypertrophic scars, keloid and Dupuytren's contracture; autoimmune diseases, e.g., systemic lupus ery-thematosus; acute or chronic 20 degenerative conditions/diseases of the eye, e.g, glaucoma, dry age-related macular degeneration, retinitis pigmentosa and other forms of heredodegenerative retinal disease, retinal detachment and tears; macular pucker, ischemia affecting the outer retina, cellular damage associated with diabetic retinopathy and retinal ischemia, damage associated with laser therapy (grid, focal, and panretinal) including photodynamic therapy, trauma, surgical 25 (retinal translocation, subretinal surgery, or vitrectomy) or light-induced iatrogenic retinopathy, and preservation of retinal transplants; ocular neovascular or edematous diseases and disorders, e.g., diabetic retinopathy, rubeosis iritis, uveitis, Fuch's heterochromatic iridocyclitis, neovascular glaucoma, corneal neovascularization, neovascularization resulting from combined vitrectomy and lensectomy, retinal ischemia, 30 choroidal vascular insufficiency, choroidal thrombosis, carotid artery ischemia, contusive ocular injury, retinopathy of permaturity, retinal vein occlusion, proliferative 31 WO 2006/088949 PCT/US2006/005312 vitreoretinopathy, corneal angiogenesis, retinal microvasculopathy, or retinal edema; connective tissue disease, e.g., rheumatoid arthritis, progressive systemic sclerosis, sjorgren's syndrome, dermatomyositis or mixed connective tissue disease; cardiac hypertrophy and heart failure; insulin resistance; amyotrophic lateral sclerosis; multiple 5 sclerosis; Alzheimer's disease; neurodegenerative diseases; preneoplastic conditions, e.g. colon polyps; and lung diseases, e.g., cystic fibrosis, chronic obstructive pulmonary disease, asthma or acute and chronic bronchitis. Such methods comprise administering to said patient a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a therapeutically effective amount of one or more compounds of formula I, II, III, III(A), 10 IV, V(A), V(B) VI(A), VI(B), VII, VII(A), VII(B), VIII(A),VIII(B), IX(A), IX(B), X(A), X(B), XI(A), XI(B), XII(A) and/or XII(B). Compounds of this invention include those in the Tables 1A, 1B, 2A, 2B, 3A, 3B, 4A, 4B, 5, 6, and 7 below (including and tautomers, isomers, prodrugs and pharmaceutically acceptable salts thereof). 15 TABLE 1A R3 0 (Y)q N NHOH RN Name R q R 3 Ex. No. N-hydroxy-2-(1,3,4,9 tetrahydro-2H-b- H zero H carbolin-2- 9 yl)pyrimidine-5 carbox-amide N-hydroxy-2-(6 methoxy-1,3,4,9- 6-OCH 3 zero H tetrahydro-2H-b- 10 carbolin-2 yl)pyrimidine-5 carboxamide 32 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2-(8 methyl-1,3,4,9 tetrahydro-2H-b- 8-Me zero H 61 carbolin-2 yl)pyrimidine-5 carboxamide N-hydroxy-2-(7 fluoro-1,3,4,9 tetrahydro-2H-b- 7-F zero H 62 carbolin-2 yl)pyrimidine-5 carboxamide N-hydroxy-2-(6 fluoro-1,3,4,9 tetrahydro-2H-b- 6-F zero H 63 carbolin-2 yl)pyrimidine-5 carboxamide N-hydroxy-2-(6 methyl-1,3,4,9 tetrahydro-2H-b- 6-Me zero H 68 carbolin-2 yl)pyrimidine-5 carboxamide N-hydroxy-2-(7 methyl-1,3,4,9 tetrahydro-2H-b- 7-Me zero H 65 carbolin-2 yl)pyrimidine-5 carboxamide N-hydroxy-2-(6 benzyloxy-1,3,4,9 tetrahydro-2H-b- 6-benzyloxy zero H carbolin-2- 64 yl)pyrimidine-5 carboxamide N-hydroxy-2-{6-[3 (morpholin-4 ylmethyl)phenyl]- 6-[3-(morpholin 1,3,4,9-tetrahydro-2H- 4- zero H 70 b-carbolin-2- ylmethyl)phenyl] yl}pyrimidine-5 carboxamide 33 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2-{6-[3 ((4-methylpiperazin-1

-

6-[3-((4 yl)methyl)phenyl]- methylpiperazin 1,3,4,9-tetrahydro-2H- I zero H 71 yl yrmiine-5- yl)methyl)phenyl] carboxamide N-hydroxy-2-{6-[4 ((4-methylpiperazin-1- 6-[4-((4 yl)methyl)phenyl]- methylpiperazin 1,3,4,9-tetrahydro-2H- 1 zero H 72 yl1py rmin-5- yl)methyl)phenyl] carboxamide N-hydroxy-2-{6-[2 ((4-methylpiperazin-1- 6-[2-((4 yl)methyl)phenyl]- methylpiperazin 1,3,4,9-tetrahydro-2H- 1 zero H 73 yl1py rmin-5- yl)methyl)phenyl] carboxamide N-hydroxy-2-{9-[2 pyrro-lidin- 1 -ylethyl]- H zero 2-(pyrrolidin-1 1,3,4,9-tetrahydro-2H- yl)eth-1-yl 23 b-carbolin-2 yl}pyrimidine-5 carboxamide N-hydroxy-2-(9 methyl-1,3,4,9- H zero CH 3 tetrahydro-2H-b- 27 carbolin-2 yl)pyrimidine-5 carboxamide N-hydroxy-2-{9-[2 pyrrolidin-1 ylmethyl]-1,3,4,9- H zero pyrrolidin- 1-yl tetrahydro-2H-b- meth-1-yl 30 carbolin-2 yl)pyrimidine-5 carboxamide N-hydroxy-2-[9 (phenylsulfonyl) 1,3,4,9-tetrahydro-2H- H 1 phenyl 26 b-carbolin-2- Y= yl)pyrimidine-5- SO 2 carboxamide 34 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2-[9 (benzyl)-1,3,4,9- IIzero benzyl tetrahydro-2H-b- 28 carbolin-2 yl)pyrimidine-5 carboxamide N-hydroxy-2- [9-(2 piperidin- 1-ylethyl)- H zero 2-(piperidin- 1 1 ,3,4,9-tetrahydro-2H- yl)eth- 1-yl 24 b-carbolin-2 yl]pyrimidine-5 carboxamide N-hydroxy-2-(9 acetyl-1,3,4,9- H 1 -CH 3 tetrahydro-2H-b- Y =- 29 carbolin-2- () yl)pyrimidine-5 carboxamide N-hydroxy-2-[9-(2 morpholin-4-ylethyl) 1,3 ,4,9-tetrahydro-2H- H zero 2-(morpholin-4 b- yl)eth-1-yl 25 carbolin-2 yl]pyrimidine-5 carboxamide N-hydroxy-2- [9-(2 hydroxyethyl)- 1,3,4,9 tetrahydro-2H-b- H zero 2-hydroxyeth- 1- 58 carbolin-2- yl yl]pyrimidine-5 carboxamide N-hydroxy-2-[9-(1 methylpiperidin-3 -yl) 1 ,3,4,9-tetrahydro-2H- 1 b- H zero methylpiperidin- 59 carbolin-2- 3 -yl yl]pyrimidine-5 carboxamide 35 WO 2006/088949 PCT/US2006/005312 TABLE IB

R

3 0 (jq N ~ NHOH N N N R" Name R q R Ex. No. N-hiydroxy-2 (1,3,4,4a,9,9a-hexahydro- H zero H 2H-b-carbolin-2 yl)pyrimidine-5-carbox amnide______ N-hydroxy-2-(6-methoxy 1,3,4,4a,9,9a-hexahydro- 6- zero H 2H-b-carbolin-2- OCH 3 yl)pyrimidine-5 carboxamide N-hydroxy-2-(6-methyl 1 ,3,4,4a,9,9a-hexahydro 2H-b-carbolin-2- 6-Me zero H 67 yl)pyrimidine-5 carboxamide N-hydroxy-2-1{9-[2 pyrrolid-in-1-ylethyl]- H zero 2-(pyrrolidin 1,3 ,4,4a,9,9a-hexahydro- 1 -yl)eth- l-yl 2H-b-carbolin-2 yl}pyrimidine-5 carboxarnide N-hydroxy-2-(9-methyl 1,3,4,4a,9,9a-hexahydro- H zero CH 3 2H-b-carbolin-2-yl)pyrimi dine-5-carboxamide N-hydroxy-2-1{9-[2 pyrrolidin- 1-ylmethyl] 1,3,4,4a,9,9a-hexahydro- H zero pyrrolidin-1 2H-b-carbolin-2- yl-meth- 1l-yl yl)pyrimidine-5 carboxamide_______ 36 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2-19 (phenylsulfonyl) 1,3,4,4a,9,9a-hexahydro- H 1 Phenyl 2H-b-carbolin-2- Y= -SO 2 yl)pyrimidine-5 carboxamide_______ N-hydroxy-2-[9-(benzyl) 1,3,4,4a,9,9a-hexahydro- H zero benzyl 2H-b-carbolin-2 yl)pyrimidine-5 carboxamide N-hydroxy-2-[9-(2 piperidin- 1-ylethyl) 1,3,4,4a,9,9a-hexahydro- H zero 2-(piperidin-1 2H-b-carbolin-2- yl)eth- l-yl yl]pyrimidine-5 carboxamide N-hydroxy-2-(9-acetyl 1,3,4,4a,9,9a-hexahydro- H 1 -CH 3 2Hbb-carbolin-2- Y =CO) yl)pyrimidine-5 carboxamide N-hydroxy-2-19-(2 morpholin-4-ylethyl) 1,3,4,4a,9,9a-hexahydro- H zero 2-(morpholin 2H-b-carbolin-2- 4-yl)eth- l-yl yl]pyrimidine-5 carboxamide TABLE 2A 0 N NHOH 5 Name Ar 2 Ex. No. N-hydroxy-2-(1,3,4,9-tetrahydro- S 2H-b-carbolin-2-yl)- 1,3 -thiazole- 19 5-carboxamide N N-hydroxy-4-(1 ,3 ,4,9-tetrahydro- phenyl 18 2H-b-carbolin-2-yl)benzamnide N-hydroxy-6-( 1,3 ,4,9-tetrahydro- 4 2H-b-carbolin-2-yl)nicotinamide / ND 37 WO 2006/088949 PCT/US2006/005312 TABLE 2B H r N NHOK Name Ar 2 Ex. No. N-hydroxy-2-(1,3,4,4a,9,9a- / s hexahydro-2H-b-carbolin-2-yl)-\/ 1,3 -thiazole-5-carboxamnide Nr N-hydroxy-4-(1 ,3 ,4,4a,9,9a- phenyl hexahydro-2H-b-carbolin-2 yl)benzamnide N-hydroxy-6-(1 ,3 ,4,4a,9,9a hexahydro-2H-b-carbolin-2 yl)nicotinamide TABLE 3A 10 R3 (Y)q N R ~ N ~N N NHOH Name R q R 3 Ex. No. N-hydroxy-2-(1 ,3,4,5 tetrahydro-2H-pyrido[4,3- H zero H 12 b]indol-2 yl)pyrimidine-5carboxamide_____ N-hydroxy-2-(8-chloro- 1,3,4,5 tetrahydro-2H-pyrido [4,3- 8CzeoH 14 b]indol-2-yl)pyrirnidine-5-8-lzr carboxamnide ___________________ 38 WO 2006/088949 PCT/US2006/005312 N-hydroxy-(-8-methyl- ,3 ,4,5' tetrahydro-2H-pyrido[ 4

,

3

-

8-CH 3 zero Hi 13 b]indol-2 carboxidne5-aidemi Nhydroxy-2-(8-foo-l ,3,4,5 tetrahydro-2H-pyrido[ 4

,

3

-

8-furo zero H 111 b bido i2nd)ormidfl-S -yl~pricarbox5-ambid d Nhydroxy-2-(8-mehX 1 ,3,4,5tethoydrO-2H,- 8-moxy3 zero H1 ttayr-Hpyrido[4, 3

-

H5 bindol-2ypyrimidife-5 carboxamide N -hydroxy-2-(7-fhiyrO1,3,4,5 tetrahydro-2H-pyrido[ 4

,

3 - -f eluo zero H1 carboxamide N-h-ydroxy2-(8-dimeth-13,4, 1,3,4,5ro-2t-trahdo[4,- 78-dimethy zero Hi5 b]indo1-2y)pyrimidifecarboxamide N-hydroxy-2- {8-m3 -(pyod 135tetrahydro-2Hd 4- ylmethoylzero Hy57 b]indo1-2-y1pyrimidiflcarboxamide N-hyroxy2-(7fluoo-1,,439 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2- { 8-(pyridin-3-yl) 1,3,4,5-tetrahydro-2H pyrido[4,3- 8-pyridin-3-yl zero H 47 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-(7-chloro-8 methyl-1,3,4,5-tetrahydro-2H pyrido[4,3- 7-chloro-8-methyl zero H 34 b]indol-2-yl)pyrimidine-5 carboxamide N-hydroxy-2-(7-bromo-1,3,4,5 tetrahydro-2H-pyrido[4,3- 7-bromo zero H 112 b]indol-2-yl)pyrimidine-5 carboxamide N-hydroxy-2-(8 trifluoromethyl-1,3,4,5 tetrahydro-2H-pyrido[4,3- 8-trifluoromethyl zero H 55 b]indol-2-yl)pyrimidine-5 carboxamide N-hydroxy-2- { 8-[4-(pyrrolidin 1-ylmethyl)phenyl]-1,3,4,5- 8-[4-(pyrrolidin-1 tetrahydro-2H-pyrido[4,3- ylmethyl)phenyl] zero H 103 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-(pyrimidin-5 yl)-1,3,4,5-tetrahydro-2H pyrido[4,3- 8-(pyrimidin-5-yl) zero H 51 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[3-(morpholin 4-ylmethyl)phenyl]-1,3,4,5- 8-[3-(morpholin-4 tetrahydro-2H-pyrido[4,3- ylmethyl)phenyl] zero H 90 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[3-(piperidin 1-ylmethyl)phenyl]-1,3,4,5- 8-[3-(piperidin-1 tetrahydro-2H-pyrido[4,3- ylmethyl)phenyl] zero H 91 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[3-(N,N dimethylaminomethyl)phenyl]- 8-[3-(NN 1,3,4,5-tetrahydro-2H- dimethylaminomethyl zero H 92 pyrido[4,3 b]indol-2-yl}pyrimidine-5 carboxamide 40 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2-{8-[4-(N acetamido)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3- acetamid lienyl] zero H 50 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-(9-chloro-8 methyl-1,3,4,5-tetrahydro-2H pyrido[4,3- 9-chloro-8-methyl zero H 54 b]indol-2-yl)pyrimidine-5 carboxamide N-hydroxy-2-(8-nitro-1,3,4,5 tetrahydro-2H-pyrido[4,3- 8-nitro zero H 37 b]indol-2-yl)pyrimidine-5 carboxamide N-hydroxy-2-{8-[3 (thiomorpholin-4 ylmethyl)phenyl]-1,3,4,5- 8-[3-(thiomorpholin- zero H 94 tetrahydro-2H-pyrido[4,3- 4-ylmethyl)phenyl] b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[3-((4 hydroxymethylpiperidin-1- 8-[3-((4 yl)methyl)phenyl]-1,3,4,5- hydroxymethylpiperi zero H 93 tetrahydro-2H-pyrido[4,3- din-i b]indol-2-yl}pyrimidine-5- yl)methyl)phenyl] carboxamide N-hydroxy-2-{8-(fur-2-yl) 1,3,4,5-tetrahydro-2H pyrido[4,3- 8-(fur-2-yl) zero H 46 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-(8-(N-acetamido) 1,3,4,5-tetrahydro-2H pyrido[4,3- 8-(N-acetamido) zero H 77 b]indol-2-yl)pyrimidine-5 carboxamide N-hydroxy-2-(8-(N methylsulfonamido)-1,3,4,5- 8-(N tetrahydro-2H-pyrido[4,3- methylsulfonamido) zero H 78 b]indol-2-yl)pyrimidine-5 carboxamide N-hydroxy-2-{8-[3-(piperazin 1-ylmethyl)phenyl]-1,3,4,5- 8-[3-(piperazin-1 tetrahydro-2H-pyrido[4,3- ylmethyl)phenyl] zero H 99 b]indol-2-yl}pyrimidine-5 carboxamide 41 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2-{8-[3-(N,N-di(2 hydroxyethyl)aminomethyl)phe 8-[3-(NN-di(2 nyl]- 1,3,4,5-tetrahydro-2H- hydroxyethyl)aNino zero H 95 pyrido[4,3 - hyrethyl)aino zer b]indol-2-yl}pyrimidine-5- methyl)phenyl] carboxamide N-hydroxy-2-(8-amino-1,3,4,5 tetrahydro-2H-pyrido[4,3- 8-amino zero H 76 b]indol-2-yl)pyrimidine-5 carboxamide N-hydroxy-2-{8-[3-((4 methylpiperazin- 1 yl)methyl)phenyl]-1,3,4,5- 8- [3ro H 96 tetrahydro-2H-pyrido[4,3- methylpen1- e b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[3-((4 (methylsulfonyl)piperazin-1 yl)methyl)phenyl]-1,3,4,5- (methylsulfonyl)piper zero H 100 tetrahydro-2H-pyrido[4,3- azin-1 b]indol-2-yl}pyrimidine-5- yl)methyl)phenyl] carboxamide N-hydroxy-2-{8-[3-((4 acetylpiperazin- 1 yl)methyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3- acetylpen- zH b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[4-(morpholin 4-ylmethyl)phenyl]-1,3,4,5- 8-[4-(morpholin-4 tetrahydro-2H-pyrido[4,3- ylmethyl)phenyl] zero H 104 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[4 (thiomorpholin-4 ylmethyl)phenyl]-1,3,4,5- 8-[4-(thiomorpholin- zero H 105 tetrahydro-2H-pyrido[4,3- 4-ylmethyl)pheny b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[3 (hydroxymethyl)phenyl]- 8-[3 1,3,4,5-tetrahydro-2H- (hydroxymethyl)phen zero H 43 pyrido[4,3 b]indol-2-yl}pyrimidine-5 carboxamide 42 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2-{8-[4 methoxyphenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3- 8-[4-methoxyphenyl] zero H 40 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{ 8-[4 methylphenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3- 8-[4-methylphenyl] zero H 41 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[4-((4 methylpiperazin- 1 yl)methyl)phenyl]-1,3,4,5- 8-[4-((4- H 106 tetrahydro-2H-pyrido[4,3- methylpen-- e b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2- { 8-isopropyl 1,3,4,5-tetrahydro-2H pyrido[4,3- 8-isopropyl zero H 38 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-tert-butyl 1,3,4,5-tetrahydro-2H pyrido[4,3- 8-tert-butyl zero H 39 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[3-(pyrrolidin 1-ylcarbonyl)phenyl]-1,3,4,5- 8-[3-(pyrrolidin-1 tetrahydro-2H-pyrido[4,3- ylcarbonyl)phenyl] zero H 42 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2- {7-[3-(pyrrolidin 1-ylmethyl)phenyl]-1,3,4,5- 7-[3-(pyrrolidin-1 tetrahydro-2H-pyrido[4,3~ ylmethyl)phenyl] zero H 121 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{7-phenyl-1,3,4,5 tetrahydro-2H-pyrido[4,3 - 7-phenyl zero H 120 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[4 (hydroxymethyl)phenyl]- 8-[4 1,3,4,5-tetrahydro-2H- (hydroxymethyl)phen zero H 107 pyrido[4,3 b]indol-2-yl}pyrimidine-5 carboxamide 43 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2-{8-[3 aminophenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3- 8-[3-aminophenyl] zero H 86 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[3 fluorophenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3- 8-[3-fluorophenyl] zero H 44 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[4 fluorophenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3- 8-[4-fluorophenyl] zero H 45 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{ 8-[fur-3-yl] 1,3,4,5-tetrahydro-2H pyrido[4,3- 8-[fur-3-yl] zero H 52 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[3-(N acetamido)phenyl]-1,3,4,5- 8-[3-(N tetrahydro-2H-pyrido[4,3- acetamido)phenyl] zero H 87 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{ 8-[3-(N methylsulfonamido)phenyl]- 8-[3-(N 1,3,4,5-tetrahydro-2H- methylsulfonamido)p zero H 88 pyrido[4,3 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[3-(N acetamidomethyl)phenyl]- 8-[3-(N 1,3,4,5-tetrahydro-2H- acetamidomethyl)phe zero H 82 pyrido[4,3 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[3-(N methylsulfonamidomethyl)phen 8-[3-(N yl]-1,3,4,5-tetrahydro-2H- methylsulfonamidom zero H 83 pyrido[4,3- typhnl b]indol-2-yl}pyrimidine-5- ethyl)phenyl] carboxamide 44 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2-{8-[3 (methylthio)phenyl]-1,3,4,5- 8-3 tetrahydro-2H-pyrido[4,3- - zero H 49 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2- { 8-[3 (methylsulfinyl)phenyl]-1,3,4,5- 8-[3 tetrahydro-2H-pyrido[4,3- (methylsulfinyl)phen zero H 79 b]indol-2-yl}pyrimidine-5- y1] carboxamide N-hydroxy-2- {84[4 dimethyla(inophenyll1-)1,3,4,5 tetrahydro-2H-pyrido[4,3- dimethylafinophenyl zero H 48 b]indol-2-yl}pyrimidine-5-] carboxamide N-hydroxy-2-{8-[34-(H tetrazol-5-yl)phenyl]-1,3,4,5- 8-[38-(H-tetrazol-5 tetrahydro-2H-pyrido[4,3- d ylaphenyl zero H 4 b]indol-2-yl}pyrimidine-5- ] carboxamide N-hydroxy-2-{8-[5-((4 methylpiperazin-1-yl)methyl)-2- [-( furyl]-1,3,4,5-tetrahydro-2H- methylpiperazin-l- zero H 108 pyrido[4,3- lmty)2fr] b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2- { 8- [5-(morpholin 4-yl)methyl-2-furyl]-1,3,4,5- 8-[5-(moholin-4 tetrahydro-2H-pyrido[4,3 yl)methyl)-2-furyl] b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[5-((4 methylpiperazin-1-yl)methyl)- 8-[5-( 4 thiien-2-yl]- 1,3 ,4,5-tetrahydro- mefflylpiperazin- 1 - zero H 110 2H-pyrido [4,3- yl)methyl-2-yl] b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[5H-pyrrol-2 yl]-1,3,4,5-tetrahydro-2H pyrido[4,3- 8-[lH-pyrro1-2-yl] zero H 53 b]indol-2-yl}pyrimidine-5 carboxamide 45 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2-{8-[thien-2-yl] 1,3,4,5-tetrahydro-2H pyrido[4,3- 8-[thien-2-yl] zero H 60 b]indol-2-yl}pyrimidine-5 carboxamide N-hydroxy-2-{8-[(4 methylpiperazin-1-yl)methyl]- 8-[(4 1,3,4,5-tetrahydro-2H- methylpiperazin-1- zero H 123 pyrido[4,3 b]indol-2-yl}pyrimidine-5- yl)methyl] carboxamide N-hydroxy-2-{5-acetyl-8 methyl-1,3,4,5-tetrahydro-2H- 1 pyrido[4,3- methyl Y = - CH 3 74 b]indol-2-yl}pyrimidine-5- C(O) carboxamide TABLE 3B H N R N N NHOH 5 Name R Ex. No. N-hydroxy-2-(1,1a,3,4,4a,5- H hexahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5carboxamide N-hydroxy-2-(8-chloro-1,1a,3,4,4a,5- 8-Cl hexahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxamide N-hydroxy-2-(8-methyl- 8-CH 3 1,3,4,4a,5,9b-hexahydro-2H- 75 pyrido[4,3-b]indol-2-yl)pyrimidine 5-carboxamide N-hydroxy-2-(8-bromo- 8-Br 1,1 a,3,4,4a,5-hexahydro-2H pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxamide N-hydroxy-2-(8-trifluoromethoxy- 8-OCF 3 1,la,3,4,4a,5-hexahydro-2H pyrido[4,3-b]indol-2-yl)pyrimidine 5-carboxamide 46 WO 2006/088949 PCT/US2006/005312 TABLE 4A H C [7-/ N-W 2 -Ar 2 --G NHOH Name W2rA G Ex. No. (2E)-N-hydroxy-3-['2- bond N -CHrICH (1 ,3,4,9-tetrahydro-2H-b- N (E) 20 carbolin-2-yl)pyrimidin 5-yllacrylamide N-hydroxy-3-12-(1,3,4,9- bond N ~ CH 2

CH

2 tetrahydro-2H-b-carbolin- N-- 21 2-yl)pyrimidin-5- N2 yl]propionamide (2E)-N-hydroxy-3-[5-

-CH

2 - /\-CH=CH (1,3 ,4,9-tetrahydro-2H-b- Ns (E)3 carbolin-2-ylmethyl) thien-2-yl]acrylamide I 5 TABLE 4B H N NHOH Name W2Arz G Ex. No. (2E)-N-hydroxy-3-[2- bodN-H (1,3,4,4a,9,9a-hexahydro- N ~ (E) 2H-b-carbolin-2 yl)pyrimidin 5-yllacrylamnide N-hydroxy-3-[2- bond N-CH 2

CH

2 (1 ,3,4,4a,9,9a-hexahydro 2H-b-carbolin-2 yl)pyrimidin-5 yl]propionamide 47 WO 2006/088949 PCT/US2006/005312 (2L}.N-hydroxy-3-[5- -CR 2 - -CH=CH (1,3,4,4a,9,9a-hexahydro- s(E) 2H-b-carbolin-2 ylmethyl)-thien-2 yllacrylamide _________________________ TABLE 5 RI N N 0 5 NHOH Name X Ex. No. N-hydroxy-6-(6,7-dimethoxy 3,4-dihydroisoquinolin-2(1Ii)- 6,7- CR 5 yl)niicotinamide dimethoxy N-hydroxy-2-(6,7-dimethoxy- 6,7- N1 3 ,4-dihydroisoquinolin-2(l11)- dimethoxy N1 yl)pyrimidine-5-carboxamide N-hydroxy-2-(7-dimethoxy-3,4-

N

dihydroisoquinolin-2(1H)- etoy N31 yl)pyrimidine-5.-carboxamide mehx TABLE 6 H N

N

N7/X NHOH 10 R Name R Ex. No. N-hydroxy-2-(1 ,4,5,6 tetrahydroazepino[4,5-b]indol-3(2H)- H 114 1 -yl)pyrimidine-5-carboxamide N-hydroxy-2-(9-methoxy- 1,4,5,6 tetrahydroazepino [4,5 -b]indol-3 (2H)- 9-methoxy 113 1 -yl)pyrimidine-5-carboxamide _________________ 48 WO 2006/088949 PCT/US2006/005312 N-hydroxy-2-(9-fluoro-1,4,5,6 tetrahydroazepino[4,5-b]indol-3(2H)- 9-fluoro 115 1-yl)pyrimidine-5-carboxamide N-hydroxy-2-(9-j3-(morphohn-4- 9-[3-(morpholin ylmethy)phenyl]-1,4,5,6 tetrahydroazepino[4,5-b]indol-3(2H)- 4- 116 1-yl)pyrimidine-5-carboxamide N-hydroxy-2-(9-[3-((4 methylpiperazin-1 -yl)methy)phenyl] 1,4,5,6-tetrahydroazepino[4,5- 1 b]indol-3(2H)-1-yl)pyrimidine-5- yl)methy)phenyl] carboxamide_________________ TABLE 7 Structure Name Example Number 2-(3,4 mdihydro[]benzothieno[2,3 N- c]pyridin-2(1R)-yl)-N N -mNHOH hydroxypyriidine-5- 118 xcarboxamide N_ 0 2-(3,4 N dihydro[l]benzofro[2,3 N NHOH c]pyridin-2(1H)-yl)-N- 119 No Nhydroxypyrimidine-5 carboxamide N- 0 2-(1H-benzo [de]isoquinolin \ N-/\ 2(311)-yl)-N- 3 N \N NHOH hydroxypyrimidine-5 S'\\ Dcarboxamide 0 N, 0 N-hydroxy-2-(10-oxo 3,4,5,1-tetrahydrobenzo[b] N NHOH 1,6-naphthyridin-2(1lH)- 122 hyl)pyrimidine-5 H carboxamide 5 Definitions Unless otherwise limited by a specific recitation herein, the following terms have the following meanings; 49 WO 2006/088949 PCT/US2006/005312 "Alkyl" refers to monovalent alkyl groups having from 1 to 10 carbon atoms, preferably from 1 to 5 carbon atoms and more preferably 1 to 3 carbon atoms. This term is exemplified by groups such as methyl, ethyl, n-propyl, iso-propyl, n-butyl, t-butyl, n-pentyl and the like. 5 "Alkylene" refers to divalent alkylene groups having from 1 to 10 carbon atoms, preferably ito 5 carbon atoms and more preferably 1 to 3 carbon atoms. This term is exemplified by groups such as methylene, 1,2-ethylene, 1,3-propylene, 1,2-propylene, 1,4 butylene, 1,5-pentylene and the like. "Substituted alkyl" refers to a monovalent alkyl group having from 1 to 3, and 10 preferably 1 to 2, substituents selected from the group consisting of alkoxy, substituted alkoxy, acyl, acylamino, amino, substituted amino, aminoacyl, aryl, substituted aryl, aryloxy, substituted aryloxy, cyano, halogen, hydroxyl, nitro, carboxyl, carboxyl ester, cycloalkyl, substituted cycloalkyl, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic, thiol, and thioalkyl. 15 "Substituted alkylene" refers to divalent alkylene group having from 1 to 3, and preferably 1 to 2, substituents selected from the group consisting of those recited for substituted alkyl. "Alkoxy" refers to the group "alkyl-O-" which includes, by way of example, methoxy, ethoxy, n-propoxy, iso-propoxy, n-butoxy, t-butoxy, sec-butoxy, n-pentoxy and 20 the like. "Substituted alkoxy" refers to the group "substituted alkyl-O-". "Acyl" refers to the groups H-C(O)-, alkyl-C(O)-, substituted alkyl-C(O)-, alkenyl C(O)-, substituted alkenyl-C(O)-, cycloalkyl-C(O)-, substituted cycloalkyl-C(O)-, aryl C(O)-, substituted aryl-C(O)-, heteroaryl-C(O)-, substituted heteroaryl-C(O), heterocyclic 25 C(O)-, and substituted heterocyclic-C(O)-. "Alkylsulfinyl" refers to the group -S(O)alkyl. "Arylsulfinyl" refers to the group -S(O)aryl. "Alkylsulfonyl" refers to the group -S(O) 2 alkyl. 50 WO 2006/088949 PCT/US2006/005312 "Arylsulfonyl" refers to the group -S(O) 2 aryl. "Aminoacyl" refers to the group -C(O)NR' 0

R'

0 where each R1 0 is independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted alkenyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, heteroaryl, substituted 5 heteroaryl, heterocyclic, substituted heterocyclic and where each R 10 is joined to fonn together with the nitrogen atom a heterocyclic or substituted heterocyclic ring. "Alkenyl" refers to a monovalent alkenyl group having from 2 to 10 carbon atoms and preferably 2 to 6 carbon atoms and having at least 1 and preferably from 1-2 sites of vinyl (double bond) unsaturation. The term "alkenyl" encompasses any and all 10 combinations of cis and trans isomers arising from the presence of unsaturation. "Alkenylene" refers to divalent alkenylene groups having from 2 to 10 carbon atoms and preferably 2 to 6 carbon atoms and having at least 1 and preferably from 1-2 sites of vinyl (double bond) unsaturation. The term "alkenylene" encompasses any and all combinations of cis and trans isomers arising from the presence of unsaturation. 15 "Substituted alkenyl" refers to alkenyl groups having from 1 to 3 substituents, and preferably 1 to 2 substituents, selected from the group consisting of alkoxy, substituted alkoxy, acyl, acylamino, amino, substituted amino, aminoacyl, aryl, substituted aryl, aryloxy, substituted aryloxy, cyano, halogen, hydroxyl, nitro, carboxyl, carboxyl esters, cycloalkyl, substituted cycloalkyl, heteroaryl, substituted heteroaryl, heterocyclic, and 20 substituted heterocyclic provided that any hydroxyl substitution is not on a vinyl carbon atom. "Substituted alken ylene" refers to divalent alkenylene group having from 1 to 3, and preferably 1 to 2, substituents selected from the group consisting of those recited for substituted alkenyl. 25 "Alkynyl" refers to a monovalent alkynyl group having from 2 to 10 carbon atoms and preferably 2 to 6 carbon atoms and having at least 1 and preferably from 1-2 sites of acetylene (triple bond) unsaturation. "Alkynylene" refers to divalent alkynylene groups having from 2 to 10 carbon atoms and preferably 2 to 6 carbon atoms and having at least 1 and preferably from 1-2 sites of 51 WO 2006/088949 PCT/US2006/005312 acetylene (triple bond) unsaturation. "Substituted alkynyl" refers to alkynyl groups having from 1 to 3 substituents, and preferably 1 to 2 substituents, selected from the group consisting of alkoxy, substituted alkoxy, acyl, acylamino, amino, substituted amino, aminoacyl, aryl, substituted aryl, 5 aryloxy, substituted aryloxy, cyano, halogen, hydroxyl, nitro, carboxyl, carboxyl esters, cycloalkyl, substituted cycloalkyl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic provided that any hydroxyl substitution is not on an acetylenic carbon atom. "Substituted alkynylene" refers to divalent alkynylene group having from 1 to 3, and 10 preferably 1 to 2, substituents selected from the group consisting of those recited for substituted alkynyl. "Amino" refers to the group -NH 2 . "Substituted amino" refers to the group -NR'R" where R' and R" are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, alkenyl, substituted 15 alkenyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic and where R' and R" are joined, together with the nitrogen bound thereto to form a heterocyclic or substituted heterocylic group provided that R' and R" are both not hydrogen. When R' is hydrogen and R" is alkyl, the substituted amino group is sometimes referred to herein as alkylamino. When R' and R" are 20 alkyl, the substituted amino group is sometimes referred to herein as dialkylamino. Also included within the term substituted amino are sulfoamido groups represented by the formula -NR'SO 2 R"' where R' is as defined above and R"' is selected from the group consisting of alkyl, substituted alkyl, aryl, substituted aryl, cycloalkyl, substituted cycloalkyl, heterocyclic, substituted heterocyclic, heteroaryl and substituted heteroaryl. 25 "Aminocarbonyloxy" refers to the group NH 2 -C(O)-O-. "Oxycarbonylamino" refers to the group alkoxy-C(O)-NH 2 - or ayloxy-C(O)-NH2-. "Acylamino" refers to the groups -NR' 1 C(O)alkyl, -NR"C(O)substituted alkyl, NR' 1 C(O)cycloalkyl, -NR' C(O)substituted cycloalkyl, -NR' C(O)alkenyl, NR"C(O)substituted alkenyl, -NR"C(O)aryl, -NR 11 C(O)substituted aryl, 52 WO 2006/088949 PCT/US2006/005312 NR"C(O)heteroaryl, -NR"C(O)substituted heteroaryl, -NR"C(O)heterocyclic, and NR" 1C(O)substituted heterocyclic where R" is hydrogen or alkyl. "Aryl" or "Ar" refers to a monovalent aromatic carbocyclic group of from 6 to 14 carbon atoms having a single ring (e.g., phenyl) or multiple condensed rings (e.g., naphthyl 5 or anthryl) which condensed rings may or may not be aromatic (e.g., 2-benzoxazolinone, 2H-1,4-benzoxazin-3(4H)-one-7-yl, and the like) provided that the point of attachment is to an aromatic ring atom. Preferred aryls include phenyl and naphthyl, e.g, 2-naphthyl. "Substituted aryl" refers to aryl groups which are substituted with from 1 to 3 substituents, and preferably 1 to 2 substituents, selected from the group consisting of 10 hydroxy, acyl, acylamino, alkyl, substituted alkyl, alkoxy, substituted alkoxy, alkenyl, substituted alkenyl, amino, substituted amino, aminoacyl, aryl, substituted aryl, aryloxy, substituted aryloxy, cycloalkoxy, substituted cycloalkoxy, carboxyl, carboxyl esters, cyano, cycloalkyl, substituted cycloalkyl, halo, nitro, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic, heteroaryloxy, substituted heteroaryloxy, 15 heterocyclyloxy, substituted heterocyclyloxy, alkylsulfinyl, arylsulfinyl, alkylsulfonyl, arylsulfonyl, thiol, and thioalkyl. "Arylene" refers to a divalent aryl group of from 6 to 14 carbon atoms having a single ring (e.g., phenylene) or multiple condensed rings (e.g., naphthylene or anthrylene) which condensed rings may or may not be aromatic (e.g., 2-benzoxazolinone, 2H-1,4 20 benzoxazin-3(4H)-one-7-yl, and the like) provided that the points of attachment are to an aromatic ring atom. Preferred arylenes include phenylene (e.g, 1,4-phenylene) and naphthylene (e.g, 1,2-naphthylene). "Substituted arylene" refers to arylene groups which are substituted with from 1 to 3 substituents, and preferably 1 to 2 substituents, selected from the group consisting of 25 hydroxy, acyl, acylamino, alkyl, substituted alkyl, alkoxy, substituted alkoxy, alkenyl, substituted alkenyl, amino, substituted amino, aminoacyl, aryl, substituted aryl, aryloxy, substituted aryloxy, cycloalkoxy, substituted cycloalkoxy, carboxyl, carboxyl esters, cyano, cycloalkyl, substituted cycloalkyl, halo, nitro, heteroaryl, substituted heteroaryl, heterocyclic, substituted heterocyclic, heteroaryloxy, substituted heteroaryloxy, 30 heterocyclyloxy, substituted heterocyclyloxy, alkylsulfinyl, arylsulfinyl, alkylsulfonyl, 53 WO 2006/088949 PCT/US2006/005312 arylsulfonyl, thiol, and thioalkyl. "Heteroarylene" refers to a divalent heteroaryl group of from 1 to 15 carbon atoms, preferably from 1 to 10 carbon atoms, and 1 to 4 heteroatoms selected from the group consisting of oxygen, nitrogen, and sulfur within the ring. Optionally, either or both of any 5 nitrogen and/or sulfur atoms within the ring can be oxidized. Such heteroaryl groups can have a single ring (e.g., pyridyl or furyl) or multiple condensed rings (e.g., indolizinyl or benzothienyl) provided that the point of attachment is through a heteroaryl ring atom. Preferred heteroaryls include pyridyl, pyrrolyl, indolyl, thiophenyl, and furyl. "Substituted heteroarylene" refers to heteroarylene groups which are substituted 10 with from 1 to 3 substituents, and preferably 1 to 2 substituents, selected from the group consisting of hydroxy, acyl, acylamino, alkyl, substituted alkyl, alkoxy, substituted alkoxy, alkenyl, substituted alkenyl, amino, substituted amino, aminoacyl, aryl, substituted aryl, aryloxy, substituted aryloxy, cycloalkoxy, substituted cycloalkoxy, carboxyl, carboxyl esters, cyano, cycloalkyl, substituted cycloalkyl, halo, nitro, heteroaryl, substituted 15 heteroaryl, heterocyclic, substituted heterocyclic, heteroaryloxy, substituted heteroaryloxy, heterocyclyloxy, substituted heterocyclyloxy, alkylsulfinyl, arylsulfinyl, alkylsulfonyl, arylsulfonyl, thiol, and thioalkyl. "1,2-fused aryl" refers to an aryl group that is fused to an optionally substituted cycloalkyl or an optionally substituted heterocyclic group at positions alpha/beta on the aryl 20 ring. Accordingly, such groups are construed as fused 1,2-aryl groups regardless of the numbering system of the aryl moiety provided that fusion is alpha/beta. For example, napthalene fused at the 2,3-positions thereof with piperidine as shown below would be construed as a fused 1,2-aryl group as the napthyl group is fused at positions alpha/beta on the naphthyl ring to the 3,4-positions of the piperidinyl group: ~- N 25 "Substituted 1,2-fused aryl" refers to fused 1,2-aryl groups substituted with 1 to 3 substituents as defined above for substituted aryl. "Aryloxy" refers to the group aryl-O- that includes, by way of example, phenoxy, 54 WO 2006/088949 PCT/US2006/005312 naphthoxy, and the like. "Substituted aryloxy" refers to substituted aryl-0- groups. "Carboxyl" refers to -COOH or pharmaceutically acceptable salts thereof. "Carboxyl esters" refers to the groups -C(0)0-alkyl, -C(0)0-substituted alkyl, 5 C(0)Oaryl, and -C(O)O-substituted aryl wherein alkyl, substituted alkyl, aryl and substituted aryl are as defined herein. "Cycloalkyl" refers to monovalent cyclic alkyl groups of from 3 to 10 carbon atoms having single or multiple condensed rings which condensed rings may or may not be cycloalkyl provided that the point of attachment is to a cycloalkyl ring atom. Examples of 10 cycloalkyl groups include, by way of example, adamantyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclooctyl and the like. "Substituted cycloalkyl" refers to a cycloalkyl group, having from 1 to 5 substituents selected from the group consisting of oxo (=0), thioxo (=S), alkyl, substituted alkyl, alkoxy, substituted alkoxy, acyl, acylamino, amino, substituted amino, aminoacyl, aryl, substituted 15 aryl, aryloxy, substituted aryloxy, cyano, halogen, hydroxyl, nitro, carboxyl, carboxyl esters, cycloalkyl, substituted cycloalkyl, heteroaryl, substituted heteroaryl, heterocyclic, and substituted heterocyclic. "Cycloalkoxy" refers to -0-cycloalkyl groups. "Substituted cycloalkoxy" refers to -0-substituted cycloalkyl groups. 20 "Halo" or "halogen" refers to fluoro, chloro, bromo and iodo and preferably is fluoro or chloro. "Heteroaryl" refers to a monovalent aromatic group of from 1 to 15 carbon atoms, preferably from 1 to 10 carbon atoms, and 1 to 4 heteroatoms selected from the group consisting of oxygen, nitrogen, and sulfur within the ring. Optionally, either or both of any 25 nitrogen and/or sulfur atoms within the ring can be oxidized. Such heteroaryl groups can have a single ring (e.g., pyridyl or furyl) or multiple condensed rings (e.g., indolizinyl or benzothienyl) provided that the point of attachment is through a heteroaryl ring atom. Preferred heteroaryls include pyridyl, pyrrolyl, indolyl, thiophenyl, and furyl. 55 WO 2006/088949 PCT/US2006/005312 "Substituted heteroaryl" refers to heteroaryl groups that are substituted with from 1 to 3 substituents selected from the same group of substituents defined for substituted aryl. "1,2-fused heteroaryl" refers to a heteroaryl group that is fused to an optionally substituted cycloalkyl or an optionally substituted heterocyclic group at positions alpha/beta 5 on the heteroaryl ring. Accordingly, such groups are construed as fused 1,2-heteroaryl groups regardless of the numbering system of the heteroaryl moiety provided that fusion is alpha/beta. For example, quinoline fused at the 2,3-positions thereof with piperidine as shown below would be construed as a fused 1,2-heteroaryl group as the quinoline group is fused at positions alpha/beta on the ring to the 3,4-positions of the piperidinyl group: 10 N "Substituted 1,2-fused heteroaryl" refers to fused 1,2-heteroaryl groups substituted with 1 to 3 substituents as defined above for substituted heteroaryl. "Heteroaryloxy" refers to the group -0-heteroaryl and "substituted heteroaryloxy" refers to the group -0-substituted heteroaryl. 15 "Heterocycle" or "heterocyclic" refers to a monovalent saturated or unsaturated group having a single ring or multiple condensed rings, from 1 to 10 carbon atoms and from 1 to 4 hetero atoms selected from the group consisting of nitrogen, sulfur and oxygen within the ring wherein, in fused ring systems, one or more the rings can be aryl or heteroaryl provided that the point of attachment is to a heterocyclic (non-aromatic) ring atom. 20 Optionally, either or both of any nitrogen and/or sulfur atoms within the ring can be oxidized. "Substituted heterocyclic" refers to heterocyclic groups that are substituted with from 1 to 3 of the same substituents as defined for substituted cycloalkyl. "1,2-fused heterocyclic" refers to a first heterocyclic group that is fused to an 25 optionally substituted cycloalkyl or an optionally substituted second heterocyclic group at positions alpha/beta on the first heterocyclic ring. Accordingly, such groups are construed as fused 1,2-heterocyclic groups regardless of the numbering system of the heterocyclic 56 WO 2006/088949 PCT/US2006/005312 moiety provided that fusion is alpha/beta. For example, indoline fused at the 2,3-positions thereof with piperidine as shown below would be construed as a fused 1,2-heterocyclic group as the indoline group is fused at positions alpha/beta on the ring to the 3,4-positions of the piperidinyl group: - rN N 5 H "Substituted 1,2-fused heterocyclic" refers to fused 1,2-heterocyclic groups substituted with 1 to 3 substituents as defined above for substituted heterocyclic. Examples of heterocycles and heteroaryls include, but are not limited to, azetidine, benzofuran, 2,3-dihydrobenzofuran, pyrrole, imidazole, pyrazole, pyridine, pyrazine, 10 pyrimidine, pyridazine, indolizine, isoindole, indole, indoline, dihydroindole, indazole, purine, quinolizine, isoquinoline, quinoline, phthalazine, naphthylpyridine, quinoxaline, quinazoline, cinnoline, pteridine, carbazole, carboline, phenanthridine, acridine, phenanthroline, isothiazole, phenazine, isoxazole, phenoxazine, phenothiazine, imidazolidine, imidazoline, piperidine, piperazine, indoline, phthalimide, 1,2,3,4-tetrahydro 15 isoquinoline, 4,5,6,7-tetrahydro-benzo[blthiophene, thiazole, thiazolidine, thiophene, benzo[b]thiophene, 2,3-dihydrobenzo[b]thiophene, morpholinyl, thiomorpholinyl (also referred to as thiamorpholinyl), piperidinyl, pyrrolidine, tetrahydrofuranyl, and the like. "Heterocyclyloxy" refers to the group -0-heterocyclic and "substituted heterocyclyloxy" refers to the group -0-substituted heterocyclic. 20 "Stereoisomer" or "stereoisomers" refer to compounds that differ in the chirality of one or more stereocenters. Stereoisomers include enantiomers and diastereomers. "Tautomer" refer to alternate forms of a compound that differ in the position of a proton, such as enol-keto and imine-enamine tautomers, or the tautomeric forms of heteroaryl groups containing a ring atom attached to both a ring -NH- moiety and a ring =N 25 moeity such as pyrazoles, imidazoles, benzimidazoles, triazoles, and tetrazoles. "Thiol" refers to the group -SH. 57 WO 2006/088949 PCT/US2006/005312 "Thioalkyl" refers to the group -S-alkyl. "Substituted thioalkyl" refers to the group -S-substituted alkyl. It is understood that in all substituted groups defined above, polymers arrived at by defining substituents with further substituents to themselves (e.g., substituted aryl having a 5 substituted aryl group as a substituent which is itself substituted with a substituted aryl group, which is further substituted by a substituted aryl group etc.) are not intended for inclusion herein. In such cases, the maximum number of such substitutions is three. For example, serial substitutions of substituted aryl groups with two other substituted aryl groups are limited to -substituted aryl-(substituted aryl)-substituted aryl. 10 Similarly, it is understood that the above definitions are not intended to include impermissible substitution patterns (e.g., methyl substituted with 5 fluoro groups). Such impermissible substitution patterns are well known to the skilled artisan. "Pharmaceutically acceptable salt" refers to pharmaceutically acceptable salts of any of the compounds of formula I, II, III, III(A), IV, V(A), V(B) VI(A), VI(B), VII, VII(A), 15 VII(B), VIII(A), VIII(B), IX(A), IX(B), X(A), X(B), XI(A), XI(B), XII(A) and/or XII(B) which salts are derived from a variety of organic and inorganic counter ions well known in the art and include, by way of example only, sodium, potassium, calcium, magnesium, ammonium, tetraalkylammonium, and the like; and when the molecule contains a basic functionality, salts of organic or inorganic acids, such as hydrochloride, hydrobromide, 20 tartrate, mesylate, acetate, maleate, oxalate and the like. The term "pharmaceutical acceptable salt thereof' also refers to the salts of the tautomers, stereoisomers, or prodrugs of I, II, III, III(A), IV, V(A), V(B) VI(A), VI(B), VII, VII(A), VII(B), VIII(A),VIII(B), IX(A), IX(B), X(A), X(B), XI(A), XI(B), XII(A) and/or XII(B). "Mammals" include human and non-human animals. 25 "Treating" or "treatment" of a disease in a patient refers to 1) preventing the disease from occurring in a patient that is predisposed or does not yet display symptoms of the disease; 2) inhibiting the disease or arresting its development; or 3) ameliorating or causing regression of the disease. "Prodrug" refers to any derivative of a compound of this invention that is capable of 58 WO 2006/088949 PCT/US2006/005312 directly or indirectly providing a compound of this invention or an active metabolite or residue thereof when administered to a subject. Particularly favored derivatives and prodrugs are those that increase the bioavailability of the compounds of this invention when such compounds are administered to a subject (e.g., by allowing an orally administered 5 compound to be more readily absorbed into the blood) or which enhance delivery of the parent compound to a biological compartment (e.g., the brain or lymphatic system) relative to the parent species. Prodrugs include ester forms of the compounds of the invention. Examples of ester prodrugs include formate, acetate, propionate, butyrate, acrylate, and ethylsuccinate derivatives. An general overview of prodrugs is provided in T. Higuchi and 10 V. Stella, Pro-drugs as Novel Delivery Systems, Vol. 14 of the A.C.S. Symposium Series, and in Edward B. Roche, ed., Bioreversible Carriers in Drug Design, American Pharmaceutical Association and Pergamon Press, 1987, both of which are incorporated herein by reference. The term "platinum coordination compound" is used herein to denote any tumor cell 15 growth inhibiting platinum coordination compound which provides platinum in the form of an ion. The term "taxane compounds" indicates a class of compounds having the taxane ring system and related to or derived form extracts from certain species of yew (Taxus) trees. 20 The term "topisomerase inhibitors" is used to indicate enzymes that are capable of altering DNA topology in eukaryotic cells. They are critical for important cellular functions and cell proliferation. There are two classes of topoisomerases in eukaryotic cells, namely type I and type II. Topoisomerase I is a monomeric enzyme of approximately 100,000 molecular weight. The enzyme binds to DNA and introduces a transient single-strand 25 break, unwinds the double helix (or allows it to unwind) and subsequently reseals the break before dissociating from the DNA strand. Topisomerase II has similar mechanism of action which involves the introduction of DNA strand breaks of the formation of free radicals. The term "camptothecin compounds" is used to indicate compounds that are related to or derived from the parent camptothecin compound which is water-insoluble alkaloid 30 derived from the Chinese tree Camptothecin acuminate and the Indian tree Nothapodytes 59 WO 2006/088949 PCT/US2006/005312 foetida. The term "podophyllotoxin compounds" is used to indicate compounds that are related to or derived from the parent podophyllotoxin, which is extracted from the mandrake plant. 5 The term "anti-tumour vinca alkaloids" is used to indicate compounds that are related to or derived from extracts of the periwinkle plant (Vinca rosea). The term "alkylating agents" encompass a diverse group of chemicals that have the common feature that they have the capacity to contribute, under physiological conditions, alkyl groups to biologically vital macromolecules such as DNA. With most of the more 10 important agents such as the nitrogen mustards and the nitrosoureas, the active alkylating moieties are generated in vivo after complex degradative reactions, some of which are enzymatic. The most important pharmacological actions of the alkylating agents are those that disturb the fundamental mechanisms concerned with cell proliferation in particular DNA synthesis and cell division. The capacity of alkylating agents to interfere with DNA 15 function and integrity in rapidly proliferating tissues provides the basis for their therapeutic applications and for many of their toxic properties. The term "anti-tumour anthracycline derivatives" comprise antibiotics obtained from the fungus Strep. peuticus var. caesius and their derivatives, characterized by having a tetracycline ring structure with an unusual sugar, daunosamine, attached by a glycosidic 20 linkage. Amplification of the human epidermal growth factor receptor 2 protein (HER 2) in primary breast carcinomas has been shown to correlate with a poor clinical prognosis for certain patients. Trastuzumab is highly purified recombinant DNA-derived humanized monoclonal IgG1 kappa antibody that binds with high affinity and specificity to the 25 extracellular domain of the HER2 receptor. Many breast cancers have estrogen receptors and growth of these tumors can be stimulated by estrogen. The terms "estrogen receptor antagonists" and "selective estrogen receptor modulators" are used to indicate competitive inhibitors of estradiol binding to the estrogen receptor (ER). Selective estrogen receptor modulators, when bound to the ER, 60 WO 2006/088949 PCT/US2006/005312 induces a change in the three-dimensional shape of the receptor, inhibiting its binding to the estrogen responsive element (ERE) on DNA. In postmenopausal women, the principal source of circulating estrogen is from conversion of adrenal and ovarian androgens (androstenedione and testosterone) to 5 estrogens (estrone and estradiol) by the aromatase enzyme in peripheral tissues. Estrogen deprivation through aromatase inhibition or inactivation is an effective and selective treatment for some postmenopausal patients with hormone-dependent breast cancer. The term "antiestrogen agent" is used herein to include not only estrogen receptor antagonists and selective estrogen receptor modulators but also aromatase inhibitors as 10 discussed above. The term "differentiating agents" encompass compounds that can, in various ways, inhibit cell proliferation and induce differentiation. Vitamin D and retinoids are known to play a major role in regulating growth and differentiation of a wide variety of normal and malignant cell types. Retinoic acid metabolism blocking agents (RAMBA's) increase the 15 levels of endogenous retinoic acids by inhibiting the cytochrome P450-mediated catabolism of retinoic acids. DNA methylation changes are among the most common abnormalities in human neoplasia. Hypermethylation within the promoters of selected genes is usually associated with inactivation of the involved genes. The term "DNA methyl transferase inhibitors" is 20 used to indicate compounds that act through pharmacological inhibition of DNA methyl transferase and reactivation of tumour suppressor gene expression. The term "kinase inhibitors" comprises potent inhibitors of kinases that are involved in cell cycle progression and programmed cell death (apoptosis). The term "farnesyltransferase inhibitors" is used to indicate compounds that were 25 designed to prevent farnesylation of Ras and other intracellular proteins. They have been shown to have effect on malignant cell proliferation and survival. The term "comprises" or "comprising" and grammatical variations thereof when used in this specification are to be taken to specify the presence of stated features, integers, steps or components or groups thereof, but do not preclude the presence or addition of one 61 WO 2006/088949 PCT/US2006/005312 or more other features, integers, steps, components or groups thereof. Compound Preparation The compounds of this invention can be prepared from readily available starting materials using the following general methods and procedures. It will be appreciated that 5 where typical or preferred process conditions (i.e., reaction temperatures, times, mole ratios of reactants, solvents, pressures, etc.) are given, other process conditions can also be used unless otherwise stated. Optimum reaction conditions may vary with the particular reactants or solvent used, but such conditions can be determined by one skilled in the art by routine optimization procedures. 10 Additionally, as will be apparent to those skilled in the art, conventional protecting groups may be necessary to prevent certain functional groups from undergoing undesired reactions. Suitable protecting groups for various functional groups as well as suitable conditions for protecting and deprotecting particular functional groups are well known in the art. For example, numerous protecting groups are described in T. W. Greene and G. M. 15 Wuts, Protecting Groups in Organic Synthesis, Third Edition, Wiley, New York, 1999, and references cited therein. Furthermore, the compounds of this invention may contain one or more chiral centers. Accordingly, if desired, such compounds can be prepared or isolated as pure stereoisomers, i.e., as individual enantiomers or diastereomers, or as stereoisomer-enriched 20 mixtures. All such stereoisomers (and enriched mixtures) are included within the scope of this invention, unless otherwise indicated. Pure stereoisomers (or enriched mixtures) may be prepared using, for example, optically active starting materials or stereoselective reagents well-known in the art. Alternatively, racemic mixtures of such compounds can be separated using, for example, chiral column chromatography, chiral resolving agents and the like. 25 The starting materials for the following reactions are generally known compounds or can be prepared by known procedures or obvious modifications thereof. For example, many of the starting materials are available from commercial suppliers such as Aldrich Chemical Co. (Milwaukee, Wisconsin, USA), Bachem (Torrance, California, USA), Emka-Chemce or Sigma (St. Louis, Missouri, USA). Others may be prepared by procedures, or obvious 30 modifications thereof, described in standard reference texts such as Fieser and Fieser's 62 WO 2006/088949 PCT/US2006/005312 Reagents for Organic Synthesis, Volumes 1-15 (John Wiley and Sons, 1991), Rodd's Chemistry of Carbon Compounds, Volumes 1-5 and Supplementals (Elsevier Science Publishers, 1989), Organic Reactions, Volumes 1-40 (John Wiley and Sons, 1991), March's Advanced Organic Chemistry, (John Wiley and Sons, 4 th Edition), and Larock's 5 Comprehensive Organic Transformations (VCH Publishers Inc., 1989). The following US patents are incorporated by reference in their entirety to the extent that they describe the synthesis of 1,2,3,4,5,6-hexahydroazepino[4,5-b]indoles: United States Patent No. 3,676,558; United States Patent No. 3,839,357; and United States Patent No. 6,407,092. 10 The following US patents are incorporated by reference in their entirety to the extent that they describe the synthesis of 1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indoles: United States Patent No. 6,090,945 and United States Patent No. 6,861,410. United States Patent No. 4,001,263 is hereby incorporated by reference in its entirety to the extent that it describes the preparation of 2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indoles. 15 United States Patent No. 4,006,164 is hereby incorporated by reference to the extent that it describes the preparation of 1,2,3,4-tetrahydropyrrolo[3,4-b]indoles. As to the synthesis of compounds of this invention, Scheme 1 below illustrates a general method for synthesis of compounds of this invention wherein W 2 and G are bonds and Lg-Ar2-COO Ra + GA 'NH - GIICN-Ar2-COO Ra 12 3 --Ar2-C(0)NHOH 20 Scheme 1 63 WO 2006/088949 PCT/US2006/005312 wherein A, Ar 2 , W, and W 1 are as defined above, Lg is a suitable leaving group such as halogen, mesylate, tosylate, sulfone, triflate and the like and Ra is alkyl or substituted alkyl and preferably is methyl or ethyl. Specifically, in Scheme 1, carboxylic acid ester 1 having a suitable leaving group 5 (LG) is combined with from about one to five equivalents of cyclic amine 2 in a suitable inert diluent. The reaction is typically conducted at an elevated temperature of from about 250 to about 180*C in a suitable solvent in the presence of a base to scavenge any acid generated during the reaction (particularly when Lg is halo). The reaction is continued for about one to 72 hours to produce an intermediate ester 3. Suitable solvents include, for 10 example, acetonitrile, tetrahydrofuran (THF), dioxane, dimethylformamide (DMF) or NN dimethylacetamide. Suitable bases include alkali metal carbonates such sodium carbonate, potassium carbonate and cesium carbonate or organic bases such as triethylamine (TEA) and diisopropylethylamine (DIEA). Upon reaction completion, compound 3 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, 15 chromatography, filtration, and the like, or, alternatively, is used in the next reaction without purification and/or isolation. Hydroxamic acids 4 are produced by treating ester 3 with an excess, typically from greater than one to about 20 equivalents of 10 to 50% hydroxylamine in a suitable solvent such as water, methanol, or ethanol. The reaction is conducted in the presence of an excess 20 of alkali metal hydroxide, typically from greater than one to about 20 equivalents of an alkali metal hydroxide. The reaction is typically conducted at from about 0 to 60'C and is continued until substantially complete which typically occurs within about one to 72 hours. Upon reaction completion, the compound 4 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, filtration, and the 25 like. Scheme 2 below illustrates a general method for synthesis of compounds of this invention wherein W 2 is methylene and G is or is not a bond and further wherein A, Ar 2 , W, W1 and Ra are as defined above. 64 WO 2006/088949 PCT/US2006/005312 NH + OHC-Ar 2 -- G-COOR= NaCNBH, A N-CH2-Ar2-G-COOR= 6 NH 2 OH CC N-CH2-Ar2-G-CONHOH Scheme 2 Specifically, in Scheme 2, reductive amination of aromatic aldehyde 5 in the presence of amine 2 provides for compound 6. Reductive amination proceeds by combining 5 approximately stoichiometric equivalents of amine 2 with aldehyde 5 in a suitable solvent such as methanol, ethanol, tetrahydrofuran, trimethylorthoformate, dioxane, and the like to provide for intermediate imine (not shown). The imine is reduced to the amine, compound 6, in situ by the presence an excess, preferably from greater than one to about ten equivalents of a borohydride reducing agent, such as sodium cyanoborohydride 10 (NaCNBH 3 ), sodium borohydride, sodium triacetoxy-borohydride and the like. The reaction is typically conducted at a temperature of from about 0 to 60'C and continued until the reaction is substantially complete which typically occurs in about 15 minutes to 48 hours. Upon reaction completion, compound 6 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, filtration, 15 and the like, or, alternatively, is used in the next reaction without purification and/or isolation. Variants of this reaction are possible and are well known in the art including the addition of acids such as, but not limited to, acetic acid or trifluoroacetic acid (TFA) and the addition of drying agents such as anhydrous magnesium or sodium sulfate. Catalytic 20 hydrogenation of the imine can also be used but is less preferred. Such hydrogenation typically uses platinum or palladium catalysts, preferably 5% palladium on carbon, and one 65 WO 2006/088949 PCT/US2006/005312 to five atmospheres of hydrogen at 20 to 80 0 C for 15 minutes to 48 hours. Suitable solvents include those recited above and preferred Ra groups include methyl and ethyl. Hydroxamic acids 7 are produced by treating ester 6 with an excess, typically from greater than one to about 20 equivalents of 10 to 50% hydroxylamine in a suitable solvent 5 such as water, methanol, or ethanol. The reaction is conducted in the presence of an excess of alkali metal hydroxide, typically from greater than one to about 20 equivalents of an alkali metal hydroxide. The reaction is typically conducted at from about 0 to 60'C and is continued until substantially complete which typically occurs within about one to 72 hours. Upon reaction completion, the compound 4 is recovered by conventional methods including 10 neutralization, evaporation, extraction, precipitation, chromatography, filtration, and the like. In Scheme 2, it is understood that further elaboration of the W 2 group (other than methylene) is well within the skill of the art. For example, alkylene aldehydes attached to the Ar 2 can be employed rather than direct aldehyde attachment. Still further, an c0 15 haloalkylene-Ar2 -G-CO 2 Ra or substituted alkylene compound can be used to provide for alkylene or substituted W2 linkage as depicted in Scheme 1. Scheme 3 below illustrates a further general method for synthesis of compounds of this invention wherein W 2 is methylene and G is or is not a bond and further wherein A, Ar2, W, W1 and Ra are as defined above. 66 WO 2006/088949 PCT/US2006/005312 a CCN-W2-Ar2-G-COOR8 W 6 N- -W2-Ar2--G-CooH 8

,N-W

2 -Ar2-G-CONHOTHP w 9 W I I A N-W2-Ar2-G-cONHoH 7 Scheme 3 Specifically, in Scheme 3, ester 6 is hydrolyzed under conventional conditions to provide for acid 8. In one embodiment, hydrolysis proceeds by addition of an excess and 5 preferably from greater than one to about 10 equivalents of a base such as an alkaline earth hydroxide including, for example, lithium hydroxide, sodium hydroxide, potassium hydroxide and the like. The reaction is conducted in a suitable solvent such as mixtures of water with ethanol, methanol and tetrahydrofuran. The reaction is typically conducted at from about 20 to 100 C and is continued until it is substantial complete which typically 10 occurs in about 15 minutes to 24 hours. Upon reaction completion, compound 8 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, filtration, and the like, or, alternatively, is used in the next reaction without purification and/or isolation. Alternatively, if the Ra group of compound 6 is a tertiary alkyl group, such as 15 tertiary-butyl, hydrolysis of this ester is accomplished by contact with about one to 50 equivalents of a strong acid such as hydrochloric acid or trifluoroacetic acid in a suitable solvent such as dichloromethane or dichloroethane. The reaction is typically conducted at from about 0 to 50 "C and is continued until it is substantial complete which typically occurs 67 WO 2006/088949 PCT/US2006/005312 in about 15 minutes to 24 hours. Upon reaction completion, compound 8 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, filtration, and the like, or, alternatively, is used in the next reaction without purification and/or isolation. 5 Carboxylic acid 8 is converted to tetrahydropyranyl protected hydroxamates 9 by contact with NH 2 OTHP in the presence of any of a number of well known peptide coupling reagents and a base. In one preferred embodiment, carboxylic acid 8 is contacted with a peptide-coupling reagent (e.g., EDCI) and about one to five equivalents of an organic base such as triethylamine or diisopropylethylamine, about one to five equivalents of HOBT, and 10 about one to three equivalents of NH 2 OTHP in a suitable solvent such as dichloromethane, acetonitrile, tetrahydofuran, dimethylformamide and the like. The reaction is typically conducted at from about 0 to 80 "C and is continued until it is substantial complete which typically occurs in about 1 to 72 hours. Upon reaction completion, compound 9 is recovered by conventional methods including neutralization, evaporation, extraction, 15 precipitation, chromatography, filtration, and the like, or, alternatively, is used in the next reaction without purification and/or isolation. The protecting group of tetrahydropyranyl protected hydroxamate 9 is then cleaved to provide for the corresponding hydroxamic acid 7. Cleavage occurs by treatment with an excess and preferably from greater than one to about 50 equivalents of a strong acid such as 20 hydrochloric acid or tetrahydrofuran in a suitable solvent such as dichloromethane, dichloroethane tetrahydrofuran and the like. The reaction is typically conducted at from about 0 to 50 "C and is continued until it is substantial complete which typically occurs in about 0.25 to 24 hours. Upon reaction completion, compound 7 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, 25 chromatography, filtration, and the like. Scheme 4 below illustrates a general method for synthesis of compounds of this invention wherein G is alkylene or alkenylene and further wherein A, Ar 2 , W, W1 and Ra are as defined above. 68 WO 2006/088949 PCT/US2006/005312 reducing 1 N-Ar-COOR agent . A N-Ar2--CHOH 3 12 1 Dess-Martin 2/0 ( '- Ar'-H CC NH + Lg-Ar2A N- -CHO WH W 2 10 11 I (Ph)a=CHCO2Ra 1 W NHOH/ W 0-Ar2 o MeOH N-Ar2 CORa 14 NHOH AIMeal 13 NHOH 1 H2/Pd-C W NHOHI N- A r2 oN H MeOH N CC N r-N- - )N- Ar2--\ NHO or <j- / ._CO0Ra 16 AIMea/ NH2OH 15 Scheme 4 Specifically, in Scheme 4, carboxylic acid ester 3 is reduced under conventional conditions to provide for alcohol 12 which is converted to aldehyde 11 under conventional 5 Dess-Martin conditions. Preferably, reduction of carboxylic acid ester 3 proceeds in the presence of an excess of reducing agent, typically from greater than 1 to about 5 equivalents, in a suitable solvent. Suitable reducing agents include, for example, diisobutylaluminum hydride, lithium aluminum hydride, and lithium borohydride. Suitable solvents include, for example, diethylether, tetrahydrofuran, dichloromethane and dioxane. 10 The reaction is typically conducted at from about -30 to 80 C and is continued until it is substantial complete which typically occurs in about 15 minutes to 24 hours. Upon reaction completion, compound 12 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, and the like; or, alternatively, used in the next step without purification and/or isolation. 15 The alcohol group of compound 12 is oxidized to the aldehyde group in compound 11 by contact with about one to five equivalents of oxidizing agents in a suitable solvent. 69 WO 2006/088949 PCT/US2006/005312 Suitable oxidizing agents include, for example, manganese dioxide, nickel oxide, and, preferably, 1,1,1-triacetoxy-1,1-dihydro-1,2-benziodoxol-3(1H)-one. Suitable solvents include, for example, dichloromethane, tetrahydrofuran, dichloroethane, and the like. The reaction is typically conducted at from about 0 to 60*C and is continued until it is 5 substantial complete which typically occurs in about 30 minutes to 24 hours. Upon reaction completion, compound 11 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, and the like; or, alternatively, used in the next step without purification and/or isolation. Alternatively, compound 11 can be prepared by contacting aldehyde 10 bearing a 10 leaving group Lg with an excess and preferably from greater than one to about five equivalents of amine 2 in a suitable solvent in the presence of a suitable base. Suitable leaving groups (Lg) include, for example, halogens, sulphones, and triflates. Suitable solvents include, for example, acetonitrile, tetrahydrofuran, dioxane, dimethylformamide or N,N-dimethylacetamide. Suitable bases include alkali metal carbonates such as sodium 15 carbonate, potassium carbonate and cesium carbonate or organic bases such as triethylamine or diisopropylethylamine. The reaction is typically conducted at from about 25 to 180'C and is continued until it is substantial complete which typically occurs in about 1 to 72 hours. Upon reaction completion, compound 11 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, and the 20 like; or, alternatively, used in the next step without purification and/or isolation. The aldehyde group of compound 11 is a functionality suitable for use in a Wittig Homer reaction to form vinyl compound 13. Specifically, aldehyde 11 is contacted with from about one to five equivalents of triphenylphosphoranylidene acetate, such as the methyl or ethyl ester, in a suitable solvent such as tetrahydrofuran, dichloromethane, 25 dioxane and the like. The reaction is typically conducted at from about 0 to 80*C and is continued until it is substantial complete which typically occurs in about 1 to 48 hours. Upon reaction completion, compound 13 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, and the like; or, alternatively, used in the next step without purification and/or isolation. 30 Conversion of carboxylic acid ester of compound 13 to the corresponding 70 WO 2006/088949 PCT/US2006/005312 hydroxamic acid 14 proceeds as described above. Alternatively, compound 13 can be treated with a mixture of about five to 20 equivalents of dry hydroxylamine hydrochloride and about 10 to 40 equivalents of trimethylaluminum in a suitable solvent at about 0 to 60C for about one to 48 hours to provide for compound 14. Suitable solvents include 5 dichloromethane, dichloroethane, and toluene. Upon reaction completion, compound 11 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, and the like; or, alternatively, used in the next step without purification and/or isolation. Optionally, the vinylene group of compound 14 can be saturated under conventional 10 conditions to provide for the ethylene group of compound 15. Preferably, hydrogenation occurs in a suitable solvent under about one to five atmospheres of hydrogen gas using about five to 200% weight of a catalyst at about 20 to 65C in about 1 to 72 hours. Suitable solvents include but are not limited to methanol, ethanol, tetrahydrofuran, ethyl acetate and the like. Suitable catalysts include, for example, palladium hydroxide, palladium on carbon, 15 platinum hydroxide and the like. Upon reaction completion, compound 15 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, and the like; or, alternatively, used in the next step without purification and/or isolation. Carboxylic acid ester 15 is converted to the corresponding hydroxamic acid by any 20 of the methods described above to provide for compound 16 which is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, and the like. Preferred Ar1/Ar 3 groups of this invention are indolyl groups fused to the nitrogen heterocycle represented by W, NH and W1 at the 2,3 positions of the indolyl ring. Scheme 25 5 below illustrates methods for substitution at the nitrogen ring atom of such preferred indolyl groups. 71 WO 2006/088949 PCT/US2006/005312 (R ) a e (R4) n 1Q ' I K N-W2-Ar 2 -COOR b R4) N-W2-Ar 2

-COOR

N W Rd N W 17MRd 18 MOH EDCI/HOBt (R4)n NHOTHP NW- O NH2OH/MOH I N-W2-Ar2-I N W NHOTHP Rd 20 1) MOH (R 4 )1 2) EDCI/HOBt W N-W2-Ar2 NH2OTHP base RdX N W NHOH Rd 19 (R4)0 W N-W2-Ar~4 N W NHOTHP H 21 CHO NHRbRc (R4)n 0 H+ N -W2-Ar2 / N-W2-A N W NHOTHP N W NHOH Rb,) 22 RbN 23 R- R' Scheme 5 wherein R 4 , W, W', W 2 , Ar 2 , THP and n are as defined above; Rb and R are independently, hydrogen, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, 5 heterocyclic or substituted heterocyclic, or Rb and Rc are joined together with the nitrogen atom pendent thereto to form a heterocyclic or substituted heterocyclic ring; Rd is -(Y)qR 3 where RW is as defined above, X is a leaving group such as a halogen and M is a metal salt such as sodium, potassium, lithium and the like. Specifically, compound 17, prepared in the manner described in Scheme 1 above, is 10 treated with a suitable base in an inert solvent at from about 0 to 60 0 C followed by addition of at least a stoichiometric equivalent of an alkylating (q is zero and R3 is alkyl or substituted alkyl), acylating (q is one, Y is carbonyl), benzoylating (q is one, Y is carbonyl 72 WO 2006/088949 PCT/US2006/005312 and R 3 is aryl or substituted aryl) or sulfonylating reagent (q is one, Y is -SO 2 -). Suitable bases include, for example, powdered sodium hydroxide, sodium hydride, potassium tertiary butoxide, potassium hydride and the like. Suitable inert solvents include but are not limited to toluene, dimethylformamide, tetrahydrofuran, and the like. The reaction is 5 typically conducted at from about 20 to 110 C and is continued until it is substantial complete which typically occurs in about 0.5 to 72 hours. Upon reaction completion, compound 18 is recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, and the like; or, alternatively, used in the next step without purification and/or isolation. 10 Hydroxamic acid 19 is produced from the corresponding carboxylic acid ester 18 by any of the methods described in any of Schemes 1-4 above. Alternatively, carboxylic acid ester 18 is hydrolyzed and converted to the corresponding tetrahydropyranyl protected hydroxamate 20 by the methods described in any of Schemes 1-4 above. Hydrolysis of the tetrahydropyranyl group of 20 is accomplished by the methods described in any of Schemes 15 1-4 to give hydroxamic acids 19. In still another alternative embodiment, carboxylic acid ester 17 is hydrolyzed and converted to the corresponding tetrahydropyranyl protected hydroxamate 21 by methods described in any of Schemes 1-4 above. Subsequent alkylation (q is zero and R 3 is alkyl or substituted alkyl), acylation (q is one, Y is carbonyl), benzoylation (q is one, Y is carbonyl 20 and R 3 is aryl or substituted aryl), or sulfonylation (q is one, Y is -SO 2 -) by the methods described for the synthesis of compound 18 provides for compound 20. Hydrolysis of the tetrahydropyranyl group of compound 20 by any of the methods described in Schemes 1-4 provides for hydroxamic acid 19. In another embodiment, treatment of tetrahydropyranyl protected hydroxamic acid 25 21 with an excess and preferably from greater than one to about five equivalents of 37% aqueous formaldehyde and an excess and preferably from greater than one to about five equivalents of an amine provides for aminomethylene intermediate 22. The reaction is typically conducted in a suitable solvent such ethanol, methanol, tetrahydrofuran, and the like at from about 20 to 1 00 0 C and is continued until it is substantial complete which 30 typically occurs in about 1 to 48 hours. Upon reaction completion, compound 22 is 73 WO 2006/088949 PCT/US2006/005312 recovered by conventional methods including neutralization, evaporation, extraction, precipitation, chromatography, and the like; or, alternatively, used in the next step without purification and/or isolation. Subsequent hydrolysis of the tetrahydropyranyl group of compound 22 by any of the methods described in Schemes 1-4 provides the 5 aminomethylene hydroxamic acid, compound 23. In another embodiment of this invention, aryl and heteroaryl substituted indoles 31 are produced as shown in Scheme 5B wherein R 4 , Rd, W 1 , W, W 2 , Ar 2 , Ra and n are defined as above and Ar4 is an optionally substituted aryl or an optionally substituted heteroaryl group. Typically, a bromo or iodo substituted indole 28 is treated with about one to three 10 equivalents of a boronic acid 29 and about 0.01 to one equivalents of a suitable palladium catalyst in the presence of about one to three equivalents of a base such as an alkali metal carbonate in a suitable solvent for about one to 72 hours at about 20 to 150 C to give the aryl and heteroaryl substituted indole esters 30. A preferred Rd is hydrogen. Examples of suitable solvents include, but are not limited to, dimethylformamide, dimethylacetamide, 15 dioxane, and tetrahydrofuran. Examples of palladium catalysts include, but are not limited to, diacetoxybis(triphenylphospine)-palladium, dichlorobis(triphenylphospine)-palladium, and tetrakis(triphenylphosphine)-palladium. Examples of suitable alkali metal carbonates include, but are not limited to, sodium, potassium or cesium carbonate. Subsequent conversion of the esters 30 to the hydroxamic acids 31 are accomplished by any one of the 20 means described in Scheme 1 to 5. 74 WO 2006/088949 PCT/US2006/005312 Br or I Ar4 (R4). Ar 4

-B(OH)

2 R4 29 N / Wi Pd-catalyst N / W1 Rd Rd W'NW 2 W' Ar2 30 NAr2 28 r2COORa 3 COORa Ar 4

(R

4 ) Ar"CHO

W?

1 (R4 )n d/N K Rd W .NW2 N 31 'Ar2 NHOH Rd W--N O w2 A2 32 aCOR 32 ECOORa NHRbRC reducing agent R4Ar4 NRR(R Ar4 NRbRC N / N / Rd W' W2 Rd N W' w2 Ar2 ~"Ar NHOH 33 COORa 34 0 Scheme 5B In another embodiment of this invention, aryl and heteroaryl substituted indoles 34 with appended aminomethyl groups are produced as shown in Scheme 5B wherein all 5 variables are defined as above. Typically, an aryl or heteroaryl substituted indole ester 32 bearing an aldehyde is produced as shown and described for Scheme 5B. Said ester 32, is reductively aminated with one to 50 equivalents of an amine, NIRbR, in a suitable solvent at from about 0* to 80 C for about one to 72 hours in the presence of about one to ten equivalents of a suitable borohydride reducing agent. Alternatively, the suitable 75 WO 2006/088949 PCT/US2006/005312 borohydride reducing agent can be replaced by about 0.05 to 1 equivalents of a suitable palladium catalyst and about one to ten atmospheres of hydrogen. Suitable solvents include, but or not limited to, methylene chloride, tetrahydrofuran, dioxane, ethanol, trimethylorthoformate, tetramethylorthoformate, ether, dichloroethane, or ethylacetate. 5 Suitable borohydride reducing reagents include, but are not limited to, sodium borohydride, sodium cyanoborohydride, and sodium triacetoxyborohydride. Suitable palladium catalysts include, but are not limited to, palladium on carbon, palladium on alumina, palladium on barium carbonate, or palladium oxide. Subsequent conversion of the ester 33 to a hydroxamic acid is accomplished by any of the means described in Scheme 1 to 5. 10 The 2,3 double bond of indolyl groups fused to the nitrogen heterocycle represented by W, NH and W1 at the 2,3 positions of the indolyl ring are particularly susceptible to hydrogenation which is depicted in Scheme 6 below. 76 WO 2006/088949 PCT/US2006/005312 (R4),, W 2 A 2 C O (R4)., N - 2 - r - O (R4)I N-W 2 -Ar 2 - COORa ( 4,N-W 2 -Ar2- COORa N W N W H 17 H 17a (R/W N- W 2-Ar2 CORRa4 W N- W 2-Ar2-COORa N W NHOT 18 18a Rd Rd IQ . / \N-W2 -Ar2 (R / WN-W2-Ar2 N W NHOH N W NHOH Rd Rd19 1 21 1 2 (R) I N- W 2 -Ar2N W2 Ar2 N W NHOTHP N W NHOTHP /20 /20a Rd Rd ( R4 (R 4 ) W N-W2 -Ar2/ NW2-Ar24 N W NHOTHP N W NHOTHP H 21 H 21 a WWi

(R

4 ), Wi1 ( WW2 -Ar2 4 N-W2-Ar2 N W NHOTHP N W NHOTHP N Rb N 22a (W)" _(R) \ / NW\ -Ar2-K

/N

2

N-W

2 -Ar2 N NHOH N W NHOH Rb.. N 23 Rb.. N 23a NRbN Rc Scheme 6 Specifically, in Scheme 6, the 2,3-double bond for each of compounds 17-23 can be hydrogenated by in a suitable solvent with about one to 20 fold weight to volume ratio of 5 trifluoroacetic acid and about one to five equivalents of a hydride reducing agent to provide 77 WO 2006/088949 PCT/US2006/005312 for the corresponding 2,3-dihydroindole derivatives, compounds 17a-23a. Suitable solvents include but are not limited to pure trifluoroacetic acid, dichloromethane, dichloroethane, tetrahydrofuran, and the like. Suitable hydride reducing agents include but are not limited to triethylsilane, sodium borohydride, and sodium cyanoborohydride. The reaction is 5 typically conducted at from about -30 to 50 C and is continued until it is substantial complete which typically occurs in about 0.5 to 72 hours. Pharmaceutical Formulations When employed as pharmaceuticals, the compounds of this invention are usually administered in the form of pharmaceutical compositions. These compounds can be 10 administered by a variety of routes including oral, rectal, transdermal, subcutaneous, intravenous, intramuscular, and intranasal. These compounds are effective as both injectable and oral compositions. Such compositions are prepared in a manner well known in the pharmaceutical art and comprise at least one active compound. This invention also includes pharmaceutical compositions which contain, as the 15 active ingredient, one or more of the compounds of this invention associated with pharmaceutically acceptable carriers. In making the compositions of this invention, the active ingredient is usually mixed with an excipient, diluted by an excipient or enclosed within such a carrier which can be in the form of a capsule, sachet, paper or other container. The excipient employed is typically an excipient suitable for administration to human 20 subjects or other mammals. When the excipient serves as a diluent, it can be a solid, semi solid, or liquid material, which acts as a vehicle, carrier or medium for the active ingredient. Thus, the compositions can be in the form of tablets, pills, powders, lozenges, sachets, cachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols (as a solid or in a liquid medium), ointments containing, for example, up to 10% by weight of the active compound, 25 soft and hard gelatin capsules, suppositories, sterile injectable solutions, and sterile packaged powders. In preparing a formulation, it may be necessary to mill the active compound to provide the appropriate particle size prior to combining with the other ingredients. If the active compound is substantially insoluble, it ordinarily is milled to a particle size of less 30 than 200 mesh. If the active compound is substantially water soluble, the particle size is 78 WO 2006/088949 PCT/US2006/005312 normally adjusted by milling to provide a substantially uniform distribution in the formulation, e.g. about 40 mesh. Alternatively, poorly water soluble compounds can be prepared in the form of nanoparticles to enhance their solubility. See, for example, International Patent Application Publication No. WO 03/024424 for "Stabilization of Active 5 Agents by Formulation into Nanoparticulate Form" which is incorporated herein by reference in its entirety. Some examples of suitable excipients include lactose, dextrose, sucrose, sorbitol, mannitol, starches, gum acacia, calcium phosphate, alginates, tragacanth, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, and 10 methyl cellulose. The formulations can additionally include: lubricating agents such as talc, magnesium stearate, and mineral oil; wetting agents; emulsifying and suspending agents; preserving agents such as methyl- and propylhydroxy-benzoates; sweetening agents; and flavoring agents. The compositions of the invention can be formulated so as to provide quick, sustained or delayed release of the active ingredient after administration to the patient 15 by employing procedures known in the art The compositions are preferably formulated in a unit dosage form. The term "unit dosage forms" refers to physically discrete units suitable as unitary dosages for human subjects and other mammals, each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect, in association with a suitable 20 pharmaceutical excipient. The compounds of the present invention maybe administered to patients either alone or in combination with other known anti-tumor agents. When administered alone about 0.005 to about 100 mg/kg, more preferably about 0.005 to about 10 mg/kg, are administered to the patient. Higher and lower dosages may be used. Administration may occur once a 25 day, or several times in a day. In addition the treatment may be repeated every 7, 14, 21 or 28 days. When administered in combination with other anti-cancer agents, the compounds of the present invention may be prepared in a formulation that includes both one or more of the compounds of this invention and one or more other anti-cancer agents. Alternatively the 30 other anti-cancer agents may be administered in a separate formulation which may be 79 WO 2006/088949 PCT/US2006/005312 administered before, after or simultaneously with the compounds of this invention. When administered in combination with at least one other anti-cancer agent, about 0.005 to about 100 mg/kg, more preferably about 0.5 to about 10 mg/kg, of one or more compounds of this invention are administered to the patient. Higher and lower dosages may be used. The 5 dosages of the other anti-cancer agents are known in the art. Administration may occur once a day, or several times in a day. In addition the treatment may be repeated every 7, 14, 21 or 28 days. The active compound is effective over a wide dosage range and is generally administered in a pharmaceutically effective amount. It, will be understood, however, that 10 the amount of the compound actually administered will be determined by a physician, in the light of the relevant circumstances, including the condition to be treated, the chosen route of administration, the actual compound administered, the age, weight, and response of the individual patient, the severity of the patient's symptoms, and the like. For preparing solid compositions such as tablets, the principal active ingredient is 15 mixed with a pharmaceutical excipient to form a solid preformulation composition containing a homogeneous mixture of a compound of the present invention. When referring to these preformulation compositions as homogeneous, it is meant that the active ingredient is dispersed evenly throughout the composition so that the composition may be readily subdivided into equally effective unit dosage forms such as tablets, pills and capsules. This 20 solid preformulation is then subdivided into unit dosage forms of the type described above containing from, for example, 0.1 to about 500 mg of the active ingredient of the present invention. The tablets or pills of the present invention may be coated or otherwise compounded to provide a dosage form affording the advantage of prolonged action. For example, the 25 tablet or pill can comprise an inner dosage and an outer dosage component, the latter being in the form of an envelope over the former. The two components can be separated by an enteric layer which serves to resist disintegration in the stomach and permit the inner component to pass intact into the duodenum or to be delayed in release. A variety of materials can be used for such enteric layers or coatings, such materials including a number 30 of polymeric acids and mixtures of polymeric acids with such materials as shellac, cetyl 80 WO 2006/088949 PCT/US2006/005312 alcohol, and cellulose acetate. The liquid forms in which the novel compositions of the present invention may be incorporated for administration orally or by injection include aqueous solutions suitably flavored syrups, aqueous or oil suspensions, and flavored emulsions with edible oils such as 5 cottonseed oil, sesame oil, coconut oil, or peanut oil, as well as elixirs and similar pharmaceutical vehicles. Compositions for inhalation or insufflation include solutions and suspensions in pharmaceutically acceptable, aqueous or organic solvents, or mixtures thereof, and powders. The liquid or solid compositions may contain suitable pharmaceutically acceptable 10 excipients as described supra. Preferably the compositions are administered by the oral or nasal respiratory route for local or systemic effect. Compositions in preferably pharmaceutically acceptable solvents may be nebulized by use of inert gases. Nebulized solutions may be breathed directly from the nebulizing device or the nebulizing device may be attached to a face masks tent, or intermittent positive pressure breathing machine. 15 Solution, suspension, or powder compositions may be administered, preferably orally or nasally, from devices which deliver the formulation in an appropriate manner. The following formulation examples illustrate the pharmaceutical compositions of the present invention. Formulation Example 1 20 Hard gelatin capsules containing the following ingredients are prepared: Quantity Ingredient (mg/capsule) Active Ingredient 30.0 Starch 305.0 Magnesium stearate 5.0 The above ingredients are mixed and filled into hard gelatin capsules in 340 mg quantities. 81 WO 2006/088949 PCT/US2006/005312 Formulation Example 2 A tablet formula is prepared using the ingredients below: Quantity Ingredient (mg/tablet) Active Ingredient 25.0 Cellulose, microcrystalline 200.0 Colloidal silicon dioxide 10.0 Stearic acid 5.0 The components are blended and compressed to form tablets, each weighing 240 5 mg. Formulation Example 3 A dry powder inhaler formulation is prepared containing the following components: Ingredient Weight % Lactose 5 Active Ingredient 95 The active mixture is mixed with the lactose and the mixture is added to a dry 10 powder inhaling appliance. Formulation Example 4 Tablets, each containing 30 mg of active ingredient, are prepared as follows: Quantity Ingredient (mg/tablet) Active Ingredient 30.0 mg Starch 45.0 mg Microcrystalline cellulose 35.0 mg Polyvinylpyrrolidone 4.0 mg (as 10% solution in water) Sodium carboxymethyl starch 4.5 mg Magnesium stearate 0.5 mg Tale 1.0 mg Total 120 mg 15 The active ingredient, starch and cellulose are passed through a No. 20 mesh U.S. 82 WO 2006/088949 PCT/US2006/005312 sieve and mixed thoroughly. The solution of polyvinyl-pyrrolidone is mixed with the resultant powders, which are then passed through a 16 mesh U.S. sieve. The granules so produced are dried at 50* to 60*C and passed through a 16 mesh U.S. sieve. The sodium carboxymethyl starch, magnesium stearate, and talc, previously passed through a No. 30 5 mesh U.S. sieve, are then added to the granules which, after mixing, are compressed on a tablet machine to yield tablets each weighing 150 mg. Formulation Example 5 Capsules, each containing 40 mg of medicament are made as follows: Quantity Ingredient (mg/capsule) Active Ingredient 40.0 mg Starch 109.0 mg Magnesium stearate 1.0 mg Total 150.0 mg 10 The active ingredient, cellulose, starch, an magnesium stearate are blended, passed through a No. 20 mesh U.S. sieve, and filled into hard gelatin capsules in 150 mg quantities. Formulation Example 6 Suppositories, each containing 25 mg of active ingredient are made as follows: Ingredient Amount Active Ingredient 25 mg Saturated fatty acid glycerides to 2,000 mg 15 The active ingredient is passed through a No. 60 mesh U.S. sieve and suspended in the saturated fatty acid glycerides previously melted using the minimum heat necessary. The mixture is then poured into a suppository mold of nominal 2.0 g capacity and allowed to cool. 83 WO 2006/088949 PCT/US2006/005312 Formulation Example 7 Suspensions, each containing 50 mg of medicament per 5.0 mL dose are made as follows: Ingredient Amount Active Ingredient 50.0 mg Xanthan gum 4.0 mg Sodium carboxymethyl cellulose (11%) 50.0 mg Microcrystalline cellulose (89%) Sucrose 1.75 g Sodium benzoate 10.0 mg Flavor and Color q.v. Purified water to 5.0 mL 5 The medicament, sucrose and xanthan gum are blended, passed through a No. 10 mesh U.S. sieve, and then mixed with a previously made solution of the microcrystalline cellulose and sodium carboxymethyl cellulose in water. The sodium benzoate, flavor, and color are diluted with some of the water and added with stirring. Sufficient water is then added to produce the required volume. 10 Formulation Example 8 Quantity Ingredient (mg/capsule) Active Ingredient 15.0 mg Starch 407.0 mg Magnesium stearate 3.0 mg Total 425.0 mg The active ingredient, cellulose, starch, and magnesium stearate are blended, passed through a No. 20 mesh U.S. sieve, and filled into hard gelatin capsules in 560 mg quantities. Formulation Example 9 15 An intravenous formulation may be prepared as follows: Ingredient Ouantity Active Ingredient 250.0 mg Isotonic saline 1000 mL 84 WO 2006/088949 PCT/US2006/005312 Formulation Example 10 A topical formulation may be prepared as follows: Ingredient Ouantity Active Ingredient 1-10 g Emulsifying Wax 30 g Liquid Paraffin 20 g White Soft Paraffin to 100 g The white soft paraffin is heated until molten. The liquid paraffin and emulsifying 5 wax are incorporated and stirred until dissolved. The active ingredient is added and stirring is continued until dispersed. The mixture is then cooled until solid. Another preferred formulation employed in the methods of the present invention employs transdermal delivery devices ("patches"). Such transdermal patches may be used to provide continuous or discontinuous infusion of the compounds of the present invention 10 in controlled amounts. The construction and use of transdermal patches for the delivery of pharmaceutical agents is well known in the art. See, e.g., U.S. Patent 5,023,252, issued June 11, 1991, herein incorporated by reference. Such patches may be constructed for continuous, pulsatile, or on demand delivery of pharmaceutical agents. Direct or indirect placement techniques may be used when it is desirable or 15 necessary to introduce the pharmaceutical composition to the brain. Direct techniques usually involve placement of a drug delivery catheter into the host's ventricular system to bypass the blood-brain barrier. One such implantable delivery system used for the transport of biological factors to specific anatomical regions of the body is described in U.S. Patent 5,011,472 which is herein incorporated by reference. 20 Indirect techniques, which are generally preferred, usually involve formulating the compositions to provide for drug latentiation by the conversion of hydrophilic drugs into lipid-soluble drugs. Latentiation is generally achieved through blocking of the hydroxy, carbonyl, sulfate, and primary amine groups present on the drug to render the drug more lipid soluble and amenable to transportation across the blood-brain barrier. Alternatively, 25 the delivery of hydrophilic drugs may be enhanced by intra-arterial infusion of hypertonic solutions which can transiently open the blood-brain barrier. 85 WO 2006/088949 PCT/US2006/005312 The following synthetic and biological examples are offered to illustrate this invention and are not to be construed in any way as limiting the scope of this invention. Unless otherwise stated, all temperatures are in degrees Celsius. EXAMPLES 5 In the examples below, the following abbreviations have the following meanings. If an abbreviation is not defined, it has its generally accepted meaning. Bm = broad multiplet Bs = broad singlet Bt = broad triplet Boc = N-tert-butoxycarbonyl d = doublet dd = doublet of doublets DCM = Dichloromethane DIEA = diisopropylethylamine DMEM = Delbaco's minimum eagle's medium DMF = NN-dimethylformanide DMSO = Dimethylsulfoxide EDC = 1-[3-(dimethylaminopropyl]- 1 ethylcarbodiimide EtOAc = ethyl acetate g pr gm = grams h = hour HOBt = N-hydroxybenzotriazole HPLC = high performance liquid chromatography HPLC % = Percent purity L = Liter LCMS or LC/MS = Liquid chromatography/mass spectrum m = multiplet M = molar M+1 = molecular weight + 1 Me = methyl MeOH = methanol min = minutes mg = milligram mL = milliliter mm = millimeter mM = millimolar mmol = millimol MHz = Megahertz N = normal nm = nanometers 86 WO 2006/088949 PCT/US2006/005312 NMR = nuclear magnetic resonance

NH

2 OTHP = O-(tetrahydro-2H-pyran-2-yl)hydroxylamine m/e or m/z = mass to charge ratio in mass spectrum q = quartet q.s. = means adding a quantity sufficient to achieve a certain state RPHPLC = reverse phase high performance liquid chromatography rt = room temperature Rt = retention time s = singlet sec = seconds t = triplet TFA = Trifluoroacetic acid THF = tetrahydrofuran TLC or tlc = thin layer chromatography w/v = weight to volume v/v = volume to volume pL = Microliter pM = Micromolar pIm = Micron All the chemicals starting materials were obtained from commercial suppliers and used without further purification. Flash column chromatography was performed with silica (60-120 mesh). Analytical 5 RPHPLC was done using Shimadzu HPLC equipped with a PDA detector using the following columns and systems: a Thermo Hypersil BDS, 4.6 x 150mm, 5 ptm particle size, C-18 column, isocratic using acetonitrile:0.1% TFA in water (60:40), flow rate = 0.5mL/min (System-1); Thermo Hypersil BDS, 4.6 x 250 mm, 5 Im particle size, C-18 column, linear gradient A-acetonitrile: B-0.1% TFA in water; 0.01min A(10%):B(90%); 10 5.00 min A(10%):B(90%); 15.00 min A(90%):B(10%); 20.00 min A(90%):B(10%); 25.00 min A(10%):B(90%); 30.00 min A(10%):B(90%); 30.00 min Stop; flow rate = 1.5 mL/min (System-2). 0 S O 15 Example 1. tert-butyl (2E)-3-(5-formylthien-2-yl)acrylate 87 WO 2006/088949 PCT/US2006/005312 A mixture of 5-bromothiophene-2-carboxaldehyde (2.41 g, 12.6 mmol), DMF (40 mL), potassium carbonate (1.75g, 12.6 mmol), tert-butyl acrylate (8 mL, 56.5 mmol), and CombiPhos-Pd6 (200 mg, CombiPhos Catalysts, Inc., P.O. Box 220, Princeton, NJ 08542) was heated in a 135 to 140 'C oil bath overnight. The solvent was evaporated, the residue 5 stirred in DCM, and the mixture filtered through a pad of diatomaceous earth. The solvent was evaporated and the residue purified by flash chromatography on silica gel eluting with 20% EtOAc/hexane to give an orange solid (1.68 g), m/e = 239 (M + 1). 'H NMR (CDCl 3 ) ppm:1.54 (9H, s), 6.36 (1H, d), 7.28 (1H, d), 7.64 (1H, d), 7.68 (1H, d), 9.9 (1H, s). -' NH I H N N S 0' 0 10 0 Example 2. (2E)-3-[5-(1,3,4,9-tetrahydro-2H-b-carbolin-2-ylmethyl)thien-2-yl]-N (tetrahydro-2H-pyran-2-yloxy)acrylamide A solution of 1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indole (160 mg) in THF was treated with Example 1 (200 mg). The solution was then treated with sodium 15 triacetoxyborohydride (260 mg) and stirred four hours. The reaction was then heated in a 50 to 60 0 C oil bath for 2 hours. Additional sodium triacetoxyborohydride (50 mg) was added and the heating continued overnight. Additional 1,2,3,4-tetrahydro-9H-pyrido[3,4 b]indole (60 mg) and sodium triacetoxyborohydride (40 mg) was added and heating continued for eight hours. The reaction was quenched with aqueous potassium carbonate 20 and extracted with EtOAc. The organics were washed with water, dried and the solvent was evaporated. The residue was purified by flash chromatography on silica gel eluting with 40% EtOAc/hexane to give tert-butyl (2E)-3-[5-(1,3,4,9-tetrahydro-2H-b-carbolin-2 ylmethyl)thien-2-yl]acrylate as an orange glass (45 mg), m/e = 395 (M + 1). A solution of tert-butyl (2E)-3-[5-(1,3,4,9-tetrahydro-2H-b-carbolin-2 25 ylmethyl)thien-2-yl]acrylate (45 mg) in DCM (1 mL) was treated with TFA (0.4 mL). After two hours, the solvent was thoroughly evaporated and the residue treated with ether to precipitate (2E)-3 -[5-(1,3,4,9-tetrahydro-2H-b-carbolin-2-ylmethyl)thien-2-yl] acrylic acid as a tan solid, m/e = 339 (M + 1). 88 WO 2006/088949 PCT/US2006/005312 A solution of (2E)-3-[5-(1,3,4,9-tetrahydro-2H-b-carbolin-2-ylmethyl)thien- 2 yl]acrylic acid in DCM (2 mL) and DIEA (0.11 mL) was sequentially treated with HOBT (32 mg), EDCI (64 mg) and NH 2 OTHP (40 mg). After three hours, the solvent was evaporated and the residue purified by flash chromatography on silica gel eluting with 5 EtOAc. (2E)-3-[5-(1,3,4,9-Tetrahydro-2H-b-carbolin-2-ylmethyl)thien-2-yl]-N-(tetrahydro 2H-pyran-2-yloxy)acrylamide was obtained as a yellow oil (37 mg), m/e = 438 (M + 1). N H N NHOH 0 Example 3. (2E)-N-hydroxy-3-[5-(1,3,4,9-tetrahydro-2H-b-carbolin-2-ylmethyl)thien 10 2-yl]acrylamide A solution of Example 2 (37 mg) in DCM (0.6 mL) was treated with TFA (0.4 mL). After four hours, the solvent was evaporated and the residue purified by preparative hplc using a linear gradient of water and acetonitrile (0 to 100 % acetonitrile). Freeze drying of the pure samples gave a floculant yellow solid (2.1 mg), m/e = 354 (M + 1). 'H NMR 15 (DMSOd 6 ) ppm: 3.05 (2H, bs), 3.8 (2H, bs), 4.45 (2H, bs), 4.75 (2H, bs), 6.23 (1H, d), 7.01 (1H, t), 7.1 (1H, t) 7.26-7.5 (5H, in), 7.59 (1H, d), 10.75 (1H, bs), 10.95 (1H, bs). 0 H OH N N N Example 4. N-hydroxy-6-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)nicotinamide 20 A mixture of methyl-6-chloronicotinate (170 mg), 1,2,3,4-tetrahydro-9H-pyrido[3,4 b]indole (170 mg), potassium carbonate (150 mg) and dioxane (6 mL) was heated in a 100 *C oil bath overnight. Another aliquote of 1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indole (170 mg) was added and the heating was continue for five hours. The reaction was cooled and partitioned between water and EtOAc. The organics were washed with water, dried, and the 89 WO 2006/088949 PCT/US2006/005312 solvent evaporated. The residue was purified by filtration through silica gel eluting with EtOAc to give methyl 6-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)nicotinate as a brown solid (75 mg), m/e = 308 (M + 1). A solution of 6-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)nicotinate (12 mg) in dioxane 5 (1.5 mL) was treated with 50% aqueous hydroxylamine (0.2 mL). After 15 minutes, iN sodium hydroxide (0.06 mL) was added and the reaction was stirred for 72 hours. The reaction was quenched with IN hydrochloric acid (0.06 mL) and the solvent evaporated. The residue was purified by preparative hplc and the pure samples freeze dried to give N hydroxy-6-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)nicotinamide as a yellow solid (4 mg), 10 m/e = 308 (M + 1). 'H NMR (DMSOd 6 ) ppm: 2.8 (2H, in), 4.05 (2H, m), 4.85 (2H, s), 6.9 7.08 (3H, m), 7.29 (1H, d), 7.39 (1H, d), 7.87-7.91 (1H, m), 8.5 (1H m), 10.9 (1H, s), 11.05 (1H, bs). A -10 0) N N 0 HN-OH 15 Example 5. 6-(6,7-dimethoxy-3,4-dihydroisoquinolin-2(I)-yl)-N hydroxynicotinamide A mixture of methyl-6-chloronicotinate (170 mg), 6,7-dimethoxy-1,2,3,4 tetrahydroisoquinoline (205 mg), potassium carbonate (150 mg) and dioxane (5 mL) was heated in a 100 C oil bath overnight. An additional aliquote of 6,7-dimethoxy-1,2,3,4 20 tetrahydroisoquinoline (100 mg) was added and heating was continued for five hours. The reaction was partitioned between water and EtOAc. The organics were washed with water, dried, and the solvent evaporated. The residue was purified by flash chromatography eluting with EtOAc to give methyl 6-(6,7-dimethoxy-3,4-dihydroisoquinolin-2(1H) yl)nicotinate as a white solid (180 mg), m/e = 329 (M + 1). 25 A solution of 6-(6,7-dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl)nicotinate (12 mg) in dioxane (0.8 mL) was treated with 50% aqueous hydroxylamine (0.2 mL). After 10 minutes the reaction was treated with IN sodium hydroxide (0.06 mL) and the solution 90 WO 2006/088949 PCT/US2006/005312 stirred for 72 hours. The reaction was quenched with 1N hydrochloric acid (0.06 mL) and the solvent was evaporated. The residue was purified by preparative hplc and the pure samples freeze dried to give 6-(6,7-dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl)-N hydroxynicotinamide as a yellow solid (7.9 mg), m/e = 330 (M + 1). 'H NMR (DMSOd 6 ) 5 ppm: 2.8 (2H, in), 3.72 (6H, s) 3.9 (2H, in), 4.7 (2H, s), 6.76 (1H, s), 6.83 (1H, s), 6.9 (1H, d), 7.9 (1H, in), 8.55 (1H, s), 11 (lH, bs). 0 N ~ OMe SIN) Example 6. methyl 2-(methylthio)pyrimidine-5-carboxylate 10 To a three necked round-bottom flask equipped with a magnetic stirrer and a reflux condenser under nitrogen, methyl 3,3-dimethoxy propionate (5.00g, 33.2mmol), anhydrous 1,2-dimethoxyethane (25ml), anhydrous methyl formate (5ml) and 60% NaH (1.70g, 42.5mmol) was added. After warming the reaction mixture at 50 C until the evolution of hydrogen gas was ceased, it was cooled in an ice/water bath and slowly allowed to reach 15 ambient temperature over night with stirring. Anhydrous diethyl ether (25 mL) was added, and the resulting suspension was filtered under nitrogen, washed with anhydrous diethyl ether (10 mL), and dried under vacuum for two hours to give 5g of sodium 3,3-dimethoxy 2-carbomethoxy-prop-1-en-1-oxide as a hydroscopic white powder. To a solution of S-methyl-iso-uronium sulphate (5.0g, 19.1 mmol) in anhydrous 20 DMF (10 mL) was added sodium 3,3-dimethoxy-2-carbomethoxyprop-1-en-1-oxide (4.5g, 22.7 mmol) and the reaction mixture was heated at 100 C under nitrogen atmosphere for one hour. The reaction mixture was brought to room temperature and water (25 mL) was added. A white solid precipitated was then collected by filtration, washed with water (10 mL) and dried under vacuum for two hours to give 1.25 g of Example 6. HPLC: (RT = 3.88 25 min.); 1 H NMR (CDCl 3 , 200MHz) ppm: 9.03 (2H, s), 3.96 (3H, s), 2.65 (3H, s). 91 WO 2006/088949 PCT/US2006/005312 0 N OMe S N I I 0 Example 7. Methyl-2-(methylsulfonyl)pyrimidine-5-carboxylate To a cooled solution of Example 6 (3.0 g, 16.3 mmol) in DCM (15 mL) was added ni-chloroperbenzoic acid (7.01 g, 40.7 mmol) and the reaction mixture was stirred at room 5 temperature. After six hours, saturated solution of NaHCO3 (15 mL) were added to the reaction mixture and stirred for 15min. The organic layer was separated, washed with saturated solution of sodium bicarbonate (15 mL), dried over sodium sulfate, filtered and concentrated to give the crude Example 7 (1.6 g), which was carried to the next reaction without purification. 'H NMR (CDCl 3 , 200MHz) ppm: 9.44 (2H, s), 4.05 (3H, s), 3.41 (3H, 10 s). 0 N OMe HI N N N Example 8. Methyl 2-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5-carboxylate To Example 7 (0.2 g, 0.869 mmol) in acetonitrile (20 mL), 1,2,3,4-tetrahydro-9H 15 pyrido[3,4-b]indole (0.175 g, 0.869 mmol) and potassium carbonate (0.220 g, 1.6 mmol) was added and refluxed at 80 'C for 12 hours. After filtering the potassium carbonate, the solvent was removed to obtain the crude ester that was purified by column chromatography using EtOAc in hexanes (50%). Rf= 0.3. 'H NMR (DMSO-d 6 , 400 MHz) ppm: 8.9 (s, 2H, 2Ar-H), 7.88 (brs, 1H, NH), 7.53 (d, 1H, J= 8Hz, lAr-H), 7.37 (d, 1H, J = 8Hz, lAr-H), 20 7.21-7.12 (in, 2H, 2Ar-H), 5.11 (s, 2H, CH 2 ), 4.35 (t, 2H, J = 4Hz, CH2), 3.91 (s, 3H, OMe), 2.94 (t, 2H, J = 4Hz, CH 2 ), m/e = 308 (M4) (M, 308.335 Calcd. for C1 7 H1 6

N

4 0 2 ). 92 WO 2006/088949 PCT/US2006/005312 0 N NHOH H N N Example 9. N-hydroxy-2-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5 carboxamide To Example 8 (0.032 g, 0.09 mmol) in MeOH (5 mL) and DCM (2 mL), aq. 5 hydroxylamine (50%, 1 mL) and aq. sodium hydroxide (100 ing in 0.5 mL of water) was added at 0 C and then let it attain room temperature over 4 hours. The solvent was evaporated, water (2 mL) was added to the residue and then acidified with HCl in ether. The resulting white precipitate was filtered and dried under vacuum for 12 hours to obtain Example 9 (25 mg, 83% yield). 'H NMR (300 MHz, CD 3 0D) ppm: 8.70 (s, 2H), 7.17 (d, J 10 = 8.7 Hz, 1H), 6.85 (s, 1H), 6.70 (in, 1H), 5.02 (in, 2H), 4.28 (m, 2H), 2.82 (in, 2H). 0 NHOH H N N N MeO Example 10. N-hydroxy-2-(6-methoxy-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxamide 15 To Example 7 (0.2 g, 0.869 mmol) in acetonitrile (20 mL), 6-methoxy-2,3,4,9 tetrahydro-1H-b-carboline (0.175 g, 0.869 mmol) and potassium carbonate (0.220 g, 1.6 mmol) was added and refluxed at 80 C for 12 hours. After filtering the potassium carbonate, the solvent was removed to obtain the crude ester that was purified by column chromatography using EtOAc in hexanes (50%). To the purified ester (0.032 g, 0.09 mmol) 20 in MeOH (5 mL) and DCM (2 mL), aq. hydroxylamine (50%, 1 mL) and aq. sodium hydroxide (100 mg in 0.5 mL of water) was added at 0 C and then let it attain room temperature over 4 hours. The solvent was removed, water (2 mL) was added to the residue 93 WO 2006/088949 PCT/US2006/005312 and then acidified with HCl in ether. The resulting white precipitate was filtered and dried under vacuum for 12 hours to obtain Example 10 (25 mg, 83%). 'H NMR (300 MHz,

CD

3 0D) ppm: 8.70 (s, 2H), 7.17 (d, J= 8.7 Hz, 1H), 6.85 (s, 1H), 6.70 (n, 1H), 5.02 (in, 2H), 4.28 (in, 2H), 2.82 (in, 2H). 5 O N - NHOH Example 11. 2-(6,7-dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl)-N hydroxypyrimidine-5-carboxamide To Example 7 (0.2 g, 0.869 mmol) in acetonitrile (20 mL), 6,7-dimethoxy-1,2,3,4 10 tetrahydroisoquinoline (0.2 g, 0.869 mmol) and potassium carbonate (0.220 g, 1.6 mmol) was added and refluxed at 80 0 C for 12 hours. After filtering the potassium carbonate, the solvent was removed to obtain the crude ester that was purified by column chromatography using EtOAc in hexanes (50%). To the purified ester (0.1 g, 0.30 mmol) in MeOH (5 mL) and DCM (2 mL), aq. hydroxylamine (50%, 1 mL) and aq. sodium hydroxide (100 mg in 15 0.5 mL of water) was added at 0 "C and then let it attain room temperature over 4 hours. The solvent was evaporated, water (2 mL) was added to the residue and then acidified with HC1 in ether. The resulting white precipitate was filtered and kept under vacuum for 12 hours to obtain Example 11 (80 mg, 80%). 1H NMR (300 MHz, DMSO-d 6 ) ppm: 11.00 (s, 1H), 9.00 (s, 1H), 8.69 (s, 2H), 6.60 (s, 1H), 6.81 (s, 1), 4.82 (in, 2H), 4.00 (t, J= 6Hz, 2H), 20 3.72 (s, 3H), 3.71 (s, 3H), 3.33 (in, 2H), 2.78 (t, J = 5.4 Hz). 0 N ' NHOH N N N H Example 12. N-hydroxy-2-(1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine 5-carboxamide 94 WO 2006/088949 PCT/US2006/005312 To Example 7 (0.2 g, 0.869 mmol) in acetonitrile (20 mL), 2,3,4,5-tetrahydro-lH pyrido[4,3-b]indole (0.149 g, 0.869 mmol) and potassium carbonate (0.220 g, 1.6 mmol) was added and refluxed at 80 'C for 12 hours. After filtering the potassium carbonate, the solvent was removed to obtain the crude ester that was purified on column chromatography 5 using EtOAc in hexanes (50%). To the purified ester (0.1 g, 0.32 mmol) in MeOH (5 mL) and DCM (2 mL), aq. hydroxylamine (50%, 1 mL) and aq. sodium hydroxide (100 mg in 0.5 mL of water) was added at 0 C and then let it attain room temperature over 4 hours. The solvent was evaporated, water (2 mL) was added to the residue and then acidified with HCl in ether. The resulting white precipitate was filtered and then purified by preparative 10 HPLC to give Example 12 (80 mg, 80%) 1 H NMR (300 MHz, CD 3 0D) ppm: 8.60 (s, 2H), 7.37 (d, J= 8.7 Hz, 1H), 7.15 (d, J= 8.7 Hz, 1H), 6.90 (in, 2H), 4.92 (in, 2H), 4.22 (in, 2H), 2.82 (in, 2H). Examples 13-16 were synthesized in the same manner as example 12 using the substituted 2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indole specified for the specific example 15 below. 0 N ~ NHOH N N N H Example 13. N-hydroxy-2-(8-methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxamide 20 From 8-methyl-2,3,4,5-tetrahydro-lH-pyrido[4,3-b]indole and Example 7 was obtained Example 13 using the method described in Example 12 (55 mg, 56% yield). HPLC: (RT = 13.64 min). 'IHNMR (CD 3 0D) ppm: 8.73 (s, 2H), 7.23 (m, 2H), 6.94 (in, 1H), 5.00 (s, 2H), 4.34 (t, 2H, J= 5.4Hz), 2.92(m, 2H), 2.42 (s, 3H); m/e = 324(M+1). 95 WO 2006/088949 PCT/US2006/005312 0 CI N NHOH N N N H Example 14. 2-(8-chloro-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)-N hydroxypyrimidine-5-carboxamide From 8-chloro-2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indole and Example 7 was 5 obtained Example 14 using the method described in Example 12 (200 mg, 96% yield). HPLC: (Rt = 13.86 min). 'IHNMR (CD 3 0D) ppm: 8.75 (s, 2H), 8.49 (s, 1H), 7.42 (s, 1H1), 7.52 (m, 1H), 7.02 (s, 1H), 5.00 (s, 2H), 4.34 (m, 2H), 2.94(m, 2H); m/e = 343.8(M+1). 0 Br N NHOH N N N H 10 Example 15. 2-(8-bromo-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)-N hydroxypyrimidine-5-carboxamide From methyl 2-(8-bromo-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine 5-carboxylate (Example 33) was obtained Example 15 using the method described in Example 12 (35 mg, 44% yield). HPLC: (RT = 13.97min). 'HNMR (CD 3 0D) ppm: 8.74 (s, 15 2H), 7.58 (m, 1H), 7.21 (m, 3H), 5.01 (s, 2H), 4.34 (m, 211), 2.94(m, 2H); m/e = 389.9(M+1). 0

F

3 CO N NHOH N N N H Example 16. N-hydroxy-2-[8-(trifluoromethoxy)-1,3,4,5-tetrahydro-2H-pyrido[4,3 20 b]indol-2-yl]pyrimidine-5-carboxamide 96 WO 2006/088949 PCT/US2006/005312 From 8-(trifluoromethoxy)-2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indole and Example 7 was obtained Example 16 using the method described in Example 12 (30 mg, 43% yield). HPLC: (RT = 14.44 min). 'HNMR (CD 3 0D) ppm: 8.74 (s, 2H), 7.33 (in, 2H), 7.01 (in, 1H), 5.03 (s, 2H), 4.35 (in, 2H), 2.95 (in, 2H); m/e = 394(M+1). 5 0 N ~ NHOH \ / N N N H Example 17. N-hydroxy-2-(8-phenyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxamide To a solution of methyl 2-(8-bromo-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 10 yl)pyrimidine-5-carboxylate (Example 33) (140mg, 0.362 mmol) in anhydrous toluene and MeOH (5:1 mL) were added tetrakistriphenylphosphine palladium (210mg, 0.181 mmol), phenylboronic acid (55mg, 0.430mmol) and 2M aqueous sodium carbonate (500mg in 3ml water) at room temperature. The reaction mixture was stirred at 100 0 C for 6 hours. After completion, the reaction mixture was diluted with water and the compound was extracted 15 with EtOAc. The combined organic layers were dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue obtained was purified by column chromatography to obtain 2-(8-phenyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxylate (35mg, 25% yield). 1 H NMR (200 MHz, CDCl 3 ) ppm: 8.90 (s, 2H), 7.78 (bs, 1H), 7.68-7.12 (in, 8H), 5.10 (s, 2H), 4.37 (t, 2H, J= 5.8Hz), 3.88 (s, 3H), 20 2.96 (in, 2H); m/e = 384.8 (M+1). To a stirred solution of 2-(8-phenyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxylate (25mg, 0.065mmol) in MeOH and DCM (15 mL: 10 mL) was added hydroxylamine hydrochloride (11.31g, 162.1mmol) at 0 "C and the mixture was stirred for 15 min. Sodium methoxide (10.53g, 195.5mmol) was added at 0-5 C under 25 nitrogen atmosphere and the mixture was stirred at room temperature for 2 hours. After completion of the reaction, the mixture was partitioned between DCM and water. It was then neutralized with 1N hydrochloric acid and the organic layer was separated. The 97 WO 2006/088949 PCT/US2006/005312 aqueous layer was extracted with DCM and the combined DCM layers were dried and concentrated to Example 17 (13 mg, 60%), HPLC (RT =14.75min). 'HNMR (CD 3 0D) ppm: 8.75 (s, 2H), 7.68-7.28 (in, 8H), 5.09 (s, 2H), 4.36 (in, 2H), 2.96(m, 2H); m/e = 385.9 (M+1). 5 Example 40-53 were prepared in the same manner as Example 17 by substituting the appropriate aryl or heteroaryl boronic acid for phenylboronic acid. 0 NHOH H N N IN Example 18. N-hydroxy-4-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)benzamide 10 To a solution of compound 1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indole (400mg, 2.32 mmol) in anhydrous DMF (20 mL) was added methyl-4-fluorobenzoate (3 90mg, 2.32 mmol), potassium carbonate (640mg, 4.64mmol) and cesium carbonate (1.5 1g, 4.64 mmol) at room temperature. The reaction mixture was then stirred at 100 0 C for 12 hours. After completion of the reaction, it was filtered and the solvent was removed under reduced 15 pressure. The residue obtained was purified by column chromatography to obtain an intermediate ester (55mg, 8% yield), HPLC: (RT = 17.43min).'H NMR (200 MHz, CDCl 3 ) ppm: 7.98 (d, 2H, J= 9.2Hz), 7.82 (bs, 1H), 7.53 (d, 1H, J= 6.4 Hz), 7.36- 6.93 (in, 5H), 4.55 (s, 2H), 4.35 (q, 2H, J= 6.8Hz), 3.87 (in, 2H), 2.94 (t, 2H, J= 5.6Hz), 1.37(t, 3H, 7.2Hz); m/e = 321 (M+1). 20 To the purified ester (0.1 g, 0.32 mmol) in MeOH (5 mL) and DCM (2 mL), aq. hydroxylamine (50%, 1 mL) and aq. sodium hydroxide (100 ing in 0.5 mL of water) was added at 0 'C and then let it attain room temperature over 4 hours. The solvent was removed, water (2 mL) was added to the residue and then acidified with HC in ether. The resulting white precipitate was filtered and dried under vacuum to obtain Example 18 (80 25 mg, 80%). 'H NMR (300 MHz, CD 3 0D) ppm: 7.68 (d, J= 8.7 Hz, 2H), 7.37 (dd, J= 32.4, 8.1 Hz, 2H), 7.05 (in, 4H), 4.52 (in, 2H), 3.79 (in, 2H), 2.89 (in, 2H). 98 WO 2006/088949 PCT/US2006/005312 / \"N N H N Ys NHOH Example 19. N-hydroxy-2-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)-1,3-thiazole-5 carboxamide 5 To methyl 2-bromo-1,3-thiazole-5-carboxylate (500 mg, 2.25 mmol) in acetonitrile (10 mL), 1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indole (387 mg, 2.25 mmol) and potassium carbonate (621 mg, 4.50 mmol) was added and refluxed at 80 "C for 4 hours. After filtering the potassium carbonate, the solvent was removed and the solid obtained was washed with EtOAc and then taken as such for the next reaction (365 mg, 52%). To the (80 mg, 0.25 10 mmol) in MeOH (5 mL) and DCM (2 mL), aq. hydroxylamine (50%, 1 mL) and aq. sodium hydroxide (100 mg in 0.5 mL of water) was added at 0 0 C and then let it attain room temperature over 4 hours. The solvent was evaporated, water (2 mL) was added to the residue and then acidified with HCl in ether. The resulting white precipitate was filtered and then dried under vacuum for 12 hours to obtain Example 19 (25 mg, 23% yield), m/e = 315 15 (M+1). 0 NHOH H N N Example 20. (2E)-N-hydroxy-3-[2-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidin 5-yl]acrylamide 20 To a stirred solution of Example 8 (2.7g, 0.0088 moles) in dry acetonitrile (30 ml) was added 4-dimethylaminopyridine (0.11g, 0.00088 moles) and Boc-anhydride (2.2 ml, 0.0096 moles) at 0*C. The temperature was slowly allowed to come to room temperature and stirring was continued for >12 hours. After completion of the reaction, acetonitrile was 99 WO 2006/088949 PCT/US2006/005312 removed in vacuum. The residue was dissolved in DCM (30 ml). The organic layer was washed with water (20 ml), brine (20 ml), dried (Na 2

SO

4 ) and concentrated. The crude residue was washed with hexane (20 ml) to give tert-butyl 2-[5 (methoxycarbonyl)pyrimidin-2-yl]-1,2,3,4-tetrahydro-9H-b-carboline-9-carboxylate (3.1g, 5 87%). Rf= 0.2. 'H NMR (DMSO-d 6 , 400 MHz) ppm: 8.8 (s, 2H, 2Ar-H), 8.06 (d, 1H, J =8Hz, 1Ar-H), 7.51 (d, 1H, J = 8Hz, 1Ar-H), 7.33-7.24 (m, 2H, 2Ar-H), 5.2 (s, 2H, CH 2 ), 4.24 (t, 2H, CH 2 ), 3.45 (s, 3H, OMe), 2.0 (brs, 2H, CH 2 ), 1.68 (s, 9H, 3CH 3 ). M/e = 409 (M+1) (M, 408.451 Calcd. for C 22

H

24

N

4 0 4 ). To a stirred solution of tert-butyl 2-[5-(methoxycarbonyl)pyrimidin-2-yl]-1,2,3,4 10 tetrahydro-9H-b-carboline-9-carboxylate (3.0g, 0.0073 moles) in dry DCM (30 mL) was added diisobutyl aluminum hydride (14.7 mL, 0.022 moles, 1.5N in DCM) at -78*C. The temperature was slowly allowed to rise to -25 C and continued the stirring for further 6.0 hours. After completion of the reaction, MeOH (20 mL) and water (20 mL) was added to the reaction and allowed to come to room temperature. The reaction mixture was then 15 extracted using DCM. The combined organic layer was washed with water (20 mL), brine (20 ml), dried (Na 2

SO

4 ) and concentrated. The compound was purified by recrystallization using mixture of diethyl ether and hexane to give pure tert-butyl 2-[5 (hydroxymethyl)pyrimidin-2-yl]- 1,2,3,4-tetrahydro-9H-b-carboline-9-carboxylate (2.1 g, 75% yield). Rf= 0.3. 'H NMR (DMSO-d 6 , 400MHz) ppm: 8.38 (s, 2H, 2Ar-H), 8.05 (d, 20 1H, J =8Hz, lAr-H), 7.49 (d, 1H, J =8Hz, lAr-H), 7.31-7.22 (m, 2H, 2Ar-H), 5.15 (s, 2H,

CH

2 ), 5.10 (t, 1H, J= 8Hz, OH), 4.34 (d, 2H, J= 8Hz, CH 2 ), 4.11 (t, 2H, CH 2 ), 2.75 (brs, 2H, CH 2 ), 1.68 (s, 9H, 3CH 3 ). M/e = 381 (M + 1) (M, 380.440 Calcd. for C 21

H

24

N

4 0 3 ). To a stirred solution of tert-butyl 2-[5-(hydroxymethyl)pyrimidin-2-yl]-1,2,3,4 tetrahydro-9H-b-carboline-9-carboxylate (2.0g, 0.0053 moles) in dry DCM (20 mL) was 25 added 1,1,1-triacetoxy-1,1-dihydro-1,2-benziodoxol-3(1H)-one (2.68g, 0.0063 moles). After stirring for 5.0 hours, Na 2

S

2 0 3 (1.0g), NaHCO 3 (1.0g) in water (15 mL) was added. The two layers were separated. The organic layer was washed with water (20 mL), dried (Na 2

SO

4 ) and concentrated. The crude product was crystallized using mixture of diethyl ether-hexane to give pure tert-butyl 2- [5-(formyl)pyrimidin-2-yl]- 1,2,3,4-tetrahydro-9H-b-carboline-9 30 carboxylate (1.4g, 74% yield). Rf= 0.4. 'H NMR (DMSO-d 6 , 400MHz) ppm: 9.8 (s, 1H, 100 WO 2006/088949 PCT/US2006/005312 CHO), 8.8 (s, 2H, 2Ar-H), 8.06 (d, 1H, J = 8Hz, lAr-H), 7.52 (d, 1H, J = 8Hz, lAr-H), 7.33-7.24 (in, 2H, 2Ar-H), 5.32 (s, 2H, CH 2 ), 4.28 (t, 2H, J= 8Hz, CH 2 ), 2.82 (t, 2H, J= 8Hz, CH 2 ), 1.69 (s, 3H, 3CH 3 ); m/e = 379 (M + 1) (M, 378.425 Caled. for C 2 1

H

22

N

4 0 3 ). To a stirred solution methyl (triphenylphosphoranylidene) acetate (2.39g, 0.0071 5 moles) in dry DCM (20 mL) was added tert-butyl 2-[5-(formyl)pyrimidin-2-yl]-1,2,3,4 tetrahydro-9H-b-carboline-9-carboxylate (1.35g, 0.0036 moles) at 0*C in dry DCM (10 mL). After stirring for >12 hours, the reaction mixture was washed with 10% NaHCO 3 solution (20 mL), brine (20 mL), dried (Na 2

SO

4 ) and concentrated. The compound was purified through silica gel column chromatography using 15 % EtOAc in hexane to give 10 tert-butyl 2-{5-[(1E)-3-methoxy-3-oxoprop-1-enyl]pyrimidin-2-yl}-1,2,3,4-tetrahydro-9H b-carboline-9-carboxylate (1.22g, 78% yield). 'H NMR (DMSO-d 6 , 400 MHz) ppm: 8.81 (s, 2H, 2Ar-H), 8.06 (d, 1H, J = 8Hz, lAr-H), 7.54 (d, 1H, J = 16Hz, =CH), 7.50 (d, 1H, J= 8Hz, lAr-H), 7.32-7.23 (m, 2H, 2Ar-H), 6.60 (d, 1H, J = 16Hz, =CH), 5.23 (s, 2H, CH 2 ), 4.19 (t, 2H, CH 2 ), 3.7 (s, 3H, OMe), 2.78 (brs, 2H, CH 2 ), 1.68 (s, 9H, 3CH 3 ). M/e = 435 (M 15 + 1) (M, 434.488 Calcd. for C 24

H

26

N

4 0 4 ). Freshly dried hydroxylamine hydrochloride (0. 14g, 0.002 moles) was suspended in dry DCM (10 mL) at 0 *C. To this was added 2 M Me 3 A1 in toluene (2.1 mL, 0.0041 moles) at 0 *C. The temperature of the clear reaction mixture was slowly taken to room temperature and tert-butyl 2-{5-[(1E)-3-methoxy-3-oxoprop-1-enyl]pyrimidin-2-yl}-1,2,3,4-tetrahydro 20 9H-b-carboline-9-carboxylate (0.15g) was added. After stirring overnight, the reaction was cooled to 0 *C and saturated solution of sodium potassium tartarate (10 mL) was added. The solid formed was filtered and purified by hplc to give Example 20 (50 mg, 43% yield). 'H NMR (DMSO-d 6 , 400 MHz) ppm: 10.93 (s, 1H, NH or OH), 10.70 (brs, 1H, NH or OH), 8.6 (s, 2H, 2Ar-H), 7.41-7.31 (in, 3H, 2Ar-H and =CH), 7.04 (t, 1H, J = 8Hz, lAr-H), 6.95 25 (t, 1H, J = 8Hz, 1Ar-H), 6.37 (d, 1H, J = 16Hz, =CH), 4.99 (s, 2H, CH 2 ), 4.20 (brs, 2H,

CH

2 ), 2.79 (brs, 2H, CH 2 ). M/e = 336 (M + 1) (M, 335.360 Calcd. for Ci 8 H1 7

N

5

O

2 ). 101 WO 2006/088949 PCT/US2006/005312 0 N NHOH H N N N Example 21. N-hydroxy-3-[2-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidin-5 yl]propanamide To a stirred solution of tert-butyl 2-{5-[(1E)-3-methoxy-3-oxoprop-1 5 enyl]pyrimidin-2-yl}-1,2,3,4-tetrahydro-9H-b-carboline-9-carboxylate (0.25g, 0.0005 moles) (See Example 20) in 10 mL of dry THF was added 1:1 mixture of Pd (OH) 2 (50 mg) & Pd/C (50 mg) and stirred in the hydrogen atmosphere using balloon. After stirring for >12 hours, the reaction was filtered through a celite pad. The filtrate was concentrated and the crude residue was purified through silica colunm using 30% EtOAc in hexane to give 10 methyl 3-{2-[9-(tert-butyloxycarbonyl)--1,3,4,9-tetrahydro-2H-b-carbolin-2-yl]pyrimidin-5 yl}propanoate (0.16g, 63% yield). 1 H NMR (DMSO-d 6 , 400MHz) ppm: 8.33 (s, 2H, 2Ar H), 8.06 (d, 1H, J = 8Hz, lAr-H), 7.49 (d, 1H, J = 8Hz, lAr-H), 7.31-7.22 (in, 2H, 2Ar-H), 5.12 (s, 2H, CH 2 ), 4.09 (t, 2H, J = 8Hz, CH 2 ), 3.57 (s, 3H, OMe), 2.74 (brs, 2H, CH 2 ), 2.7 (t, 2H, CH 2 ), 2.67 (t, 2H, CH 2 ), 1.68 (s, 9H, 3CH 3 ). M/e = 437 (M+1) (M, 436.504 Calcd. 15 for C2 4

H

28

N

4 0 4 ). Freshly dried hydroxylamine hydrochloride (0.14g, 0.002 moles) was suspended in dry DCM (10 mL) at 0 *C. To this was added 2 M Me 3 A1 in toluene(2.1 mL, 0.0041 moles) at 0 *C. The temperature of the clear reaction mixture was slowly taken to room temperature and methyl 3-{2-[9-(tert-butyloxycarbonyl)-1,3,4,9-tetrahydro-2H-b-carbolin-2 20 yl]pyrimidin-5-yl}propanoate (0.15g) was added. After stirring overnight, the reaction was cooled to 0 'C and saturated solution of sodium potassium tartarate (10 mL) was added. The solid formed was filtered and purified through HPLC to give Example 21 (40mg, 34% yield). 'H NMR (DMSO-d 6 , 400 MHz) ppm: 10.9 (s, 1H, NH or OH), 10.35 (s, 1H, NH or OH), 8.27 (s, 2H, 2Ar-H), 7.38 (d, 1H, J = 4Hz, lAr-H), 7.31 (d, 1H, J = 8Hz, lAr-H), 7.05 25 6.94 (in, 2H, 2Ar-H), 4.89 (s, 2H, CH 2 ), 4.11 (t, 2H, J = 8Hz, CH 2 ), 4.75 (brs, 2H, CH 2 ), 2.66 (t, 2H, J = 8Hz, CH 2 ), 2.21 (t, 2H, J = 8Hz, CH 2 ); m/e = 338 (M + 1) (M, 337.376 102 WO 2006/088949 PCT/US2006/005312 Calcd. for C 18

H

19

N

5 0 2 ). 0 NHOH H N N Example 22. (2E)-N-hydroxy-3-[4-(1,3,4,9-tetrahydro-2H-b-carbolin-2 5 yl)phenyllacrylamide To a solution of 1,2,3,4-tetrahydro-9H-pyrido[3,4-b]indole (250 mg, 1.460 mmol) in dry DMF (10 mL) were added 4-fluorobenzaldehyde (181 mg, 1.460 mmol) and CsF (266mg, 1.752 mmol) and the reaction mixture was stirred at 65 0 C for 5 hours. After completion, the reaction mixture was diluted with water (25 mL) and EtOAc (25 mL). The 10 organic layer was separated and the aqueous layer was washed twice with EtOAc. The combined organic layers were dried over anhydrous sodium sulphate and concentrated under vacuum to provide the crude compound. It was then purified by column chromatography using silica gel to obtain 4-(1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)benzaldehyde (125mg). 1 H NMR (CDCl 3

+DMSO-D

6 , 200 MHz) ppm: 9.93 (1H, bs), 15 9.74 (111, s), 7.99 (1H, s), 7.76 (211, d, J= 8.8 Hz), 7.44 (1H, in), 7.32 (1H, in), 7.13-6.97 (3H, m), 4.61 (2H, s), 3.86 (2H, t, J= 5.6Hz), 2.93 (211, in); m/e = 277 (M+1). To a solution of compound 4-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)benzaldehyde (125 mg, 0.453 mmniol) in dry acetonitrile (15 ml) was added (triphenylphosphoranylidene) acetate (166 mg, 0.489 mmol) and the reaction mixture was stirred at 65 0 C for 3 hours. 20 After completion, the solvent was evaporated and the mixture was diluted with water (25 mL) and EtOAc (25 mL). The organic layer was separated and the aqueous layer was washed with EtOAc (25 mL). The combined organic layers were dried over anhydrous sodium sulfate and concentrated under vacuum to provide the crude compound. It was then purified by column chromatography using silica gel to obtain methyl (2E)-3-[4-(1,3,4,9 25 tetrahydro-2H-b-carbolin-2-yl)phenyl]acrylate (55mg). HPLC: (RT = 17.11 min); 1H NMR (CDCl 3 , 200 MHz) ppm: 7.84 (111, s), 7.65 (1H, d, J= 15.8 Hz), 7.49-7.08 (611, in), 6.91 (211, d, J= 9.2Hz), 6.28 (111, d, J = 15.8Hz), 4.50 (211, s), 3.79-3.73 (511, in), 2.93 (211, t, J 103 WO 2006/088949 PCT/US2006/005312 5.8Hz); m/e = 333 (M+1). To the purified ester methyl (2E)-3-[4-(1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)phenyl]acrylate (0.1 g, 0.32 mmol) in MeOH (5 mL) and DCM (2 mL), aq. hydroxyl amine (50%, 1 mL) and aq. sodium hydroxide (100 mg in 0.5 mL of water) was added at 0 5 "C and then let it attain room temperature over 4 hours. The solvent was removed, water (2 mL) was added to the residue and then acidified with HCl in ether. The resulting white precipitate was filtered and dried under vacuum to obtain Example 22 (80 mg, 80% yield). 'H NMR (300 MHz, CD 3 0D) ppm: 7.43-7.28 (m, 6H), 7.04-6.91 (in, 5H), 6.22 (d, J= 15.9 Hz, 1H), 4.52 (in, 2H), 3.79 (in, 2H), 2.80 (in, 2H). 10 N O N N NHOH (SICN N Example 23. N-hydroxy-2-{9-[2-pyrrolidin-1-ylethyl]-1,3,4,9-tetrahydro-2H-b carbolin-2-yl}pyrimidine-5-carboxamide To Example 8 (200 ing, 0.649 mmol) in toluene (20 mL) chloro-2-(pyrrolidin-1 15 yl)ethane (166 mg, 0.973 mmol) and powdered sodium hydroxide (51 mg, 1.29 mmol) was added and heated at 110 0 C for 12 hours. It was then poured into water and extracted with EtOAc. The residue obtained on removal of solvent was taken as such for next hydroxamate reaction. To the crude ester (50 mg, 0.12 mmol) in MeOH, aq. hydroxylamine (50%, 1 mL) and aq. sodium hydroxide (100 mg in 0.5 mL of water) was added at 0 C and then let it 20 attain room temperature over 4 hours. The solvent was removed, water (2 mL) was added to the residue and then acidified with HCl in ether. The resulting white precipitate was filtered and then purified by hplc to obtain Example 23 (10 mg, 20%) 'H NMR (300 MHz, CD 3 0D) ppm: 8.70 (s, 2H), 7.47 (in, 2H), 7.25 (m, 1H), 7.10 (in, 1H), 6.90 (in, 1H), 5.02 (m, 2H), 4.62 (in, 2H), 4.26 (in, 2H), 3.60-3.80 (in, 4H), 3.20 (in, 2H), 2.82 (in, 2H), 2.02-2.30 (in, 25 4H). 104 WO 2006/088949 PCT/US2006/005312 N 0 N NHOH N NN Example 24. N-hydroxy-2-[9-(2-piperidin-1-ylethyl)-1,3,4,9-tetrahydro-2H-b-carbolin 2-yllpyrimidine-5-carboxamide To sodium hydride (38 mg, 1.62 mmol) in anhydrous DMF (10 mL) was added 5 Example 8 (200 mg, 0.6 mmol, in 5 mL DMF) with stirring at room temperature. After stirring the reaction mixture for 1 hour, chloro-2-(piperidin-1-yl)ethane (149 mg, 1.62 mmol) in 3 mL DMF was added and the reaction mixture was stirred for a further 16 hours. The solvent was removed under reduced pressure, water (10 mL) was added and the pH was adjusted to 2 using 2 N HCl and then extracted with EtOAc. The organic layer was dried 10 over anhydrous sodium sulfate, filtered and concentrated under reduced pressure to give the 2-[9-(2-piperidin-1-ylethyl)-1,3,4,9-tetrahydro-2H-b-carbolin-2-yl]pyrimidine-5-carboxylic acid (200 mg, 78 % yield), m/e = 420 (M+1). To a solution of the acid 2-[9-(2-piperidin-1-ylethyl)-1,3,4,9-tetrahydro-2H-b carbolin-2-yl]pyrimidine-5-carboxylic acid (200mg, 0.49 mmol) in DCM (20 mL) was 15 added EDCI (140 mg, 0.73 mmol), HOBT (99 mg, 0.73 mmol), DIEA (183 mg, 1.46 mmol) and NH 2 OTHP (57 mg, 0.48 mmol) under nitrogen atmosphere. The reaction mixture was stirred at room temperature for 16 hours. The reaction mixture was concentrated under reduced pressure and the residue obtained was purified by column chromatography using EtOAc in hexanes (50%) to obtain N-(tetrahydro-2H-pyran-2-yloxy)-2-[9-(2-piperidin-1 20 ylethyl)-1,3,4,9-tetrahydro-2H-b-carbolin-2-yl]pyrimidine-5-carboxamide (80 mg). 'H NMR (200 MHz, CDC1 3 ) ppm: 8.75 (s, 2H), 7.50 (d, 1H, J= 7.0 Hz), 7.30 (m, 1H), 7.16 (m, 2H), 5.13 (s, 2H), 5.04 (s, 1H), 4.24 (m, 4H), 4.00 (m, 1H), 2.91 (m, 2H), 2.70 (m, 2H), 2.49 (m, 4H), 1.85 -1.45 (m, 11H); m/e = 504 (M+1). To a solution N-(tetrahydro-2H-pyran-2-yloxy)-2- [9-(2-piperidin- 1 -ylethyl)- 1,3,4,9 25 tetrahydro-2H-b-carbolin-2-yl]pyrimidine-5-carboxamide (75 mg) in MeOH (1 mL) was added 18% HC1 in ether (5mL) at 0 "C. The reaction mixture was stirred at 0 0 C for 15 min. 105 WO 2006/088949 PCT/US2006/005312 Solvent was removed under reduced pressure. To the crude product, ether was added and the precipitated solid was filtered and dried to give Example 24 (15 mg). 'H NMR (CD 3 0D, 200 MHz) ppm: 8.83 (s, 2H), 7.54 (in, 2H), 7.25 (in, 1H), 7.16 (in, 2H), 5.21 (s, 2H), 4.60 (in, 2H), 4.33 (in, 2H), 3.67 (in, 4H), 3.52-2.93 (in, 6H), 2.93 (m, 2H), 1.98 (in, 6H); m/e 5 421 (M+1). HPLC: (RT: 12.81 min). 0 N 0 N NHOH N N Example 25. N-hydroxy-2-[9-(2-morpholin-4-ylethyl)-1,3,4,9-tetrahydro-2H-b carbolin-2-yl]pyrimidine-5-carboxamide 10 To sodium hydride (140 mg, 5.83 mmol) in anhydrous DMF (10 mL) was added Example 8 (200 mg, 0.6 mmol, in 10 mL DMF) with stirring at room temperature. After stirring the reaction mixture for 1 houor, 4-(2-chloroethyl) morpholine (172 mg, 1.62 mmol) in 3 mL DMF was added to it and the reaction mixture was stirred for further 16 hours. The solvent was removed under reduced pressure, water (10 mL) was added and the pH was 15 adjusted to 2 using 2 N HCl and then extracted with EtOAc. The organic layer was dried over anhydrous sodium sulphate, filtered and concentrated under reduced pressure to give the N-hydroxy-2-[9-(2-morpholin-4-ylethyl)-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl]pyrimidine-5-carboxylic acid (160 mg, 68 % yield), m/e = 407 (M+1). To a solution of 2-[9-(2-morpholin-4-ylethyl)-1,3,4,9-tetrahydro-2H-b-carbolin-2 20 yl]pyrimidine-5-carboxylic acid (160mg, 0.39 mmol) in DCM (20 mL) were added EDCI (170 mg, 0.88 mmol), HOBT (90 mg, 0.66 mmol), DIEA (152 mg, 1.17 mmol) and

NH

2 OTHP (47 mg, 0.4 mmol) under nitrogen atmosphere. The reaction mixture was stirred at room temperature for 16 hours. The reaction mixture was concentrated under reduced pressure and the residue obtained was purified by column chromatography using EtOAc in 25 hexanes (50%) to obtain N-(tetrahydro-2H-pyran-2-yloxy)-2-[9-(2-morpholin-4-ylethyl) 1,3,4,9-tetrahydro-2H-b-carbolin-2-yl]pyrimidine-5-carboxamide (46 mg, 23 % yield). 1H 106 WO 2006/088949 PCT/US2006/005312 NMR (200 MHz, CDCl 3 ) ppm: 8.75 (s, 2H), 7.50 (d, 1H, J= 7.0 Hz), 7.30 (in, 1H), 7.16 (in, 2H), 5.13 (s, 2H), 5.04 (s, 1H), 4.24 (m, 4H), 4.00 (in, 1H), 3.69 (in, 4H), 2.91 (in, 2H), 2.70 (m, 2H), 2.49 (in, 4H), 1.85 (in, 2H), 1.47 (m, 5H); m/e = 506 (M+1). To a solution of N-(tetrahydro-2H-pyran-2-yloxy)-2-[9-(2-morpholin-4-ylethyl) 5 1,3,4,9-tetrahydro-2H-b-carbolin-2-yl]pyrimidine-5-carboxamide (46 mg) in MeOH (1 mL) was added 18% HCl in ether (4 mL) at 0 'C. The reaction mixture was stirred at 0 'C for 15 min. The solvent was removed under reduced pressure. To the crude residue obtained, ether was added and the precipitated solid was filtered and dried to give Example 25 (24 mg, 63 % yield). 'H NMR (CD 3 0D, 200 MHz) ppm: 8.85 (s, 2H), 7.54 (in, 2H), 7.25 (m, 1H), 7.14 10 (m, 2H), 5.24 (s, 2H), 4.99 (in, 2H), 4.70 (in, 2H), 4.36 (m, 2H), 4.12 (in, 2H), 3.95 (in, 2H), 3.63 (in, 4H), 2. 94 (in, 2H); m/e = 422 (M+1). HPLC: (RT: 12.94 min). O N 0 NHOH NNN Example 26. N-hydroxy-2-[9-(phenylsulfonyl)-1,3,4,9-tetrahydro-2H-b-carbolin-2 15 yl]pyrimidine-5-carboxamide To a stirred solution of Example 8 (150 mg, 0.487mmol) in THF (15 mL) was added potassium t-butoxide (81 mg, 0.720mmol) and 18-crown-6 (2mg) at 0 0 C. The suspension was stirred for 10-15 min at 0 'C and then benzenesulfonyl chlorde (0.86mg, 0.486mmol) was added. The reaction mixture was further stirred at 0 0 C for 30 min. and then stirred at 20 room temperature for 3 hours. The reaction mixture was diluted with EtOAc and water. The organic layer was washed with saturated sodium bicarbonate, dried over sodium sulfate and concentrated under vacuum. The crude product was purified by column chromatography using EtOAc in hexanes (50%) to obtain methyl 2-[9-(phenylsulfonyl)-1,3,4,9-tetrahydro 2H-b-carbolin-2-yl]pyrimidine-5-carboxylate (60 mg). 'HNMR (200 MHz, DMSO-d 6 ) ppm: 25 8.8 (s, 2H), 8.14-7.25 (in, 9H), 5.42 (s, 2H), 4.26 (t, 2H, J= 5.4Hz), 3.91 (s, 3H), 2.81( 2H, t, J= 5.4Hz); m/e = 449 (M+1). 107 WO 2006/088949 PCT/US2006/005312 To a stirred solution of methyl 2-[9-(phenylsulfonyl)-1,3,4,9-tetrahydro-2H-b carbolin-2-yl]pyrimidine-5-carboxylate (50 mg) in MeOH and DCM (5 mL, 3:2) was added hydroxylamine hydrochloride (6.42 g, mmol) at room temperature. After 15 min, sodium methoxide (4.45g, mmol) was added at 0-5 *C under nitrogen atmosphere and the reaction 5 mixture was stirred at room temperature for 3 hours. Water was added (5 mL) and neutralized with 1N hydrochloric acid and the organic layer was separated. The aqueous layer was extracted once with DCM (50 mL) and the combined organic layer was dried and concentrated to obtain Example 26 (25 mg). 'HNMR (DMSO-d 6 ) ppm: 8.78 (s, 2H), 8.13 (in, 1H), 7.94 (in, 1H), 7.61-7.26 (in, 7H), 5.43 (s, 2H), 4.27 (t, 2H, J= 5.3Hz), 2.79( 2H, t, 10 J= 5.3Hz); m/e = 449.9 (M+1); HPLC: (RT = 15.33 min). 0 N ~ NHOH N N Example 27. N-hydroxy-2-(9-methyl-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxamide 15 To a stirred solution of Example 8 (200 mg, mmol) in DMF (5 mL) was added potassium t-butoxide (145 mg) at 0 "C. After stirring the suspension for 10-15 min at 0 C, methyl iodide (0.11 g, mmol) was added. The mixture was stirred at 0 "C for 30 min. and then stirred at room temperature for 3 hours. After completion, the reaction mixture was diluted with EtOAc. The organic layer was separated, washed with saturated sodium 20 bicarbonate, dried over sodium sulfate and concentrated under vacuum. The crude product obtained was purified by column chromatography using EtOAc in hexanes (50%) to obtain methyl 2-(9-methyl-1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5-carboxylate (160 mg, 76.5% yield). 'HNMR (DMSO-d 6 ) ppm: 2.8 (t, 2H, CH 2 ); 3.65 (s, 3H, N-CH 3 ); 3.8 (s, 3H, OCH 3 ); 4.2 (t, 2H, CH 2 ); 5.15 (s, 2H, CH 2 ); 7.0-7.2 (in, 2H); 7.4 (d, 2H); 8.8 (s, 2H); 25 m/e = 323 (M+1). To a stirred solution of methyl 2-(9-methyl-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxylate (160 mg, mmol) in MeOH and DCM (5 mL, 3:2) was added 108 WO 2006/088949 PCT/US2006/005312 hydroxylamine hydrochloride (10.3 g, mmol) at room temperature and stirred for 15 min. Sodium methoxide (5.3g, mmol) was added at 0-5 0 C under nitrogen atmosphere and the mixture was stirred at room temperature for 3 hours. After completion of the reaction, the mixture was partitioned between DCM and water, neutralized with 1N hydrochloric acid 5 and the organic layer was separated. The aqueous layer was extracted with DCM (50 mL) and the combined organic layers were dried and concentrated to obtain Example 27 (60 mg, 37.5% yield). 'HNMR (200 MHz, DMSO-d 6 ) ppm: 2.8 (t, 2H); 3.65 (s, 3H, N-CH 3 ); 4.2 (t, 2H); 5.00 (s, 2H); 6.95-7.2 (m, 2H); 7.4 (d, 2H); 8.7 (s, 2H); m/e = 323.9 (M+1). N NHOH N0N N 10 Example 28. N-hydroxy-2-(9-benzyl-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxamide To a stirred solution of Example 8 (200 mg, mmol) in DMF (mL) was added potassium t-butoxide (150 mg) at 0 0 C. After stirring at 0 'C for 15 min., benzyl bromide 15 (0.1 mL, mmol) was added slowly. After completion of addition, the reaction mixture was allowed to attain room temperature and stirred for a further 3 hours. After completion of reaction, the reaction mixture was diluted with EtOAc. The organic layer was separated and washed with saturated sodium bicarbonate and water. After drying the organic layer using sodium sulfate, it was concentrated to obtain a crude product that was then purified by 20 column chromatography to obtain methyl 2-(9-benzyl-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxylate (200 mg, 77.0 % yield), m/e = 398.9 (M+1). To a stirred solution of methyl 2-(9-benzyl-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxylate (100 mg) in THF and water (5 mL each) was added lithium hydroxide (23 mg, mmol) at room temperature. The mixture was stirred for 3 hours at room 25 temperature and after which it was acidified with 1N hydrochloric acid. It was then extracted with ethyl acetate and concentrated to obtain the 2-(9-benzyl-1,3,4,9-tetrahydro 2H-b-carbolin-2-yl)pyrimidine-5-carboxylic acid (80 mg, 83% yield), m/e = 382.9 (M-1). 109 WO 2006/088949 PCT/US2006/005312 To a stirred solution of 2-(9-benzyl-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxylic acid (90 mg) in DCM (5 mL) was added EDCI (74 mg) at 0 0 C and the reaction mixture was stirred for 20 min. NH 2 OTHP (30 mg) was added and the temperature was allowed to attain room temperature. After stirring at room temperature 5 overnight, it was diluted with DCM. The organic layer was washed with water and then concentrated under vacuum. The residue obtained was purified by column chromatography to obtain N-(tetrahydro-2H-pyran-2-yloxy)-2- [9-benzyl- 1,3,4,9-tetrahydro-2H-b-carbolin-2 yl]pyrimidine-5-carboxamide (60 mg, 53% yield), m/e = 484 (M+1). A solution of N-(tetrahydro-2H-pyran-2-yloxy)-2-[9-benzyl-1,3,4,9-tetrahydro-2H 10 b-carbolin-2-yl]pyrimidine-5-carboxamide (60 mg, mmol) was stirred with hydrogen chloride in ether (5 mL) at 0-5 'C for 30 min. The product that separated out was filtered and washed with ether to obtain Example 28 (19 mg, 38%). 1 H NMR (200 MHz, DMSO d 6 ) ppm: 2.8 (t, 2H); 4.2 (t, 2H); 5.0 (s, 2H); 5.4 (s, 2H); 7.0-7.6 (in, 9H); 8.7 (s, 2H); m/e = 400.0 (M+1). 15 0 0 N NHOH N N N Example 29. 2-(9-acetyl-1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)-N-hydroxypyrimidine 5-carboxamide To a stirred solution of Example 8 (500 mg, mmol) in THF and water (10 mL, 1:1) 20 was added lithium hydroxide (205 mg, mmol) at room temperature and the mixture was stirred for 4-5 hours. After completion, the reaction mixture was acidified using 1N hydrochloric acid. The mixture was extracted with EtOAc, washed with brine, the organics dried over sodium sulfate and concentrated to give 2-(1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxylic acid (400 mg, 83% yield). 25 To a stirred solution of 2-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5 carboxylic acid (400 mg) in DCM (10 mL) was added EDCI (390 mg, mmol) at 0 "C. After stirring the reaction mixture at 0 "C for 15 min. NH 2 OTHP (160 mg, mmol) was added. 110 WO 2006/088949 PCT/US2006/005312 After stirring overnight, the reaction was diluted with DCM (30 mL). The organic layer was washed with saturated sodium bicarbonate and water. The crude product obtained on removal of solvent was purified by column chromatography using EtOAc in hexanes (50%) to obtain N-(tetrahydro-2H-pyran-2-yloxy)-2-[1,3,4,9-tetrahydro-2H-b-carbolin-2 5 yl]pyrimidine-5-carboxamide (250 mg, 46.8% yield), m/e = 394 (M+1). To a stirred solution of N-(tetrahydro-2H-pyran-2-yloxy)-2-[1,3,4,9-tetrahydro-2H b-carbolin-2-yl]pyrimidine-5-carboxamide (120 mg, mmol) in DMF (5 mL) was added sodium hydride (22 mg) at 0 'C and the mixture was stirred for 20 min. Acetyl chloride (30 mg, rmol) was added and the reaction was allowed to attain room temperature and stirred 3 10 hours. After completion, the reaction mixture was partitioned between EtOAc and water. The organic layer was separated and washed with water, saturated sodium bicarbonate and concentrated. Pure N-(tetrahydro-2H-pyran-2-yloxy)-2-[9-acetyl-1,3,4,9-tetrahydro-2H-b carbolin-2-yl]pyrimidine-5-carboxamide (80 mg, 60% yield) was obtained by column chromatography m/e = 436 (M+1). 15 A solution of N-(tetrahydro-2H-pyran-2-yloxy)-2-[9-acetyl-1,3,4,9-tetrahydro-2H-b carbolin-2-yl]pyrimidine-5-carboxamide (80 mg, mmol) was stirred in hydrogen chloride in ether (5 mL) for 25-30 min at 0 C and the solid that separated out was filtered. The product was washed with ether and dried under vacuum to obtain Example 29 (30 mg, 46.8% yield). 'HNMR (200 MHz, CD 3 0D) ppm: 2.8 (s, 3H, CH3); 2.9 (t, 2H, CH2); 4.3 (t, 2H); 5.4 (s, 20 2H); 7.2-7.4 (in, 2H); 7.5 (in, 1H); 7.9 (d, 1H); 8.8 (s, 2H); m/e = 352 (M++1). 0 N N NHOH N N Example 30. N-hydroxy-2-{9-[2-pyrrolidin-1-ylmethyl]-1,3,4,9-tetrahydro-2H-b carbolin-2-yl}pyrimidine-5-carboxamide 25 A solution of N-(tetrahydro-2H-pyran-2-yloxy)-2-[1,3,4,9-tetrahydro-2H-b-carbolin 2-yl]pyrimidine-5-carboxamide (See intermediate in Example 29) (100 mg, 0.26 mmol) in EtOH (0.6 mL) was treated 37% aqueous formaldehyde (0.025 mL) and pyrrolidine (22 mg) ill WO 2006/088949 PCT/US2006/005312 and heated to reflux overnight. The reaction mixture was cooled, diluted with water, and extracted with EtOAc. The extracts were washed with water, dried and the solvent evaporated to give N-(tetrahydro-2H-pyran-2-yloxy)-2-[9- [2-pyrrolidin- 1 -ylmethyl] 1,3,4,9 tetrahydro-2H-b-carbolin-2-yl]pyrimidine-5-carboxamide (60 mg), m/e = 477 (M + 1). 5 A solution of N-(tetrahydro-2H-pyran-2-yloxy)-2-[9-[2-pyrrolidin-1 ylmethyl] 1,3,4,9-tetrahydro-2H-b-carbolin-2-yl]pyrimidine-5-carboxamide (60 mg) was stirred in hydrogen chloride in ether (5 mL) for 25-30 min at 0 0 C and the solid that separated out was filtered. The product was washed with ether and dried under vacuum to obtain Example 30 (40 mg), m/e = 393 (M + 1). 10 Examples 31 and 32 were prepared in the same manner as Example 11 by substituting the appropriate tetrahydroisoquinoline for 6,7-dimethoxy-1,2,3,4 tetrahydroisoquinoline. N-- NHOH

H

3 CO 15 Example 31. 2-(7-methoxy-3,4,dihydroisoquinolin-2(1H)-yl)-N-hydroxypyrimidine-5 carboxamide From 7-methoxy-1,2,3,4-tetrahydroisoquinoline and Example 7 was obtained the trifluoroacetic acid salt of Example 31 as a floculant white solid after hplc purification. 'H NMR (300MHz, DMSO-d 6 ) ppm: 2.82 (in, 2H), 3.73 (s, 3H), 4.00 (in, 2H), 4.89 (s, 2H), 20 6.75 (in, 1H), 6.86 (in, 1H), 7.08 (in, 1H), 8.75 (s, 2H), 11.1 (bs, 1H); m/e = 301 (M + 1). / \ -- N--/ O4 N NHOH Example 32. 2-(1H-benzo[de]isoquinolin-2(3H)-yl)-N-hydroxypyrimidine-5 carboxamide 25 From 2,3-dihydro-1H-benzo[de]isoquinoline and Example 7 was obtained Example 112 WO 2006/088949 PCT/US2006/005312 32. 'H NMR (300 MHz, DMSO-D 6 ): 5.34 (s, 4H), 7.47 (in, 4H), 7.79 (m, 2H), 8.69 (s, 2H), 8.70 (S, 2H), m/e = 307 (M+1). 0 Br N-

OCH

3 \ / N N 5 Example 33. methyl 2-(8-bromo-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxylate To a stirred solution of 4-bromophenylhydrazine hydrochloride (5.0g, 22.36 mmol) in ethanol (100 mL), piperidin-4-one hydrochloride (1.316g, 8.5984 mmol) was added and the mixture was heated to reflux. The reflux was maintained for 4 hours, then cooled to 10 room temperature and dry hydrogen chloride gas was passed for 1 hour through the reaction mixture. The mixture was then again heated to reflux and maintained for 2h. After completion of reaction, ethanol was distilled off under vacuum and the residue was dissolved in water. The aqueous layer was neutralized with 2N sodium hydroxide solution and was extracted in dichloromethane (2 x 100 mL). The pH of the aqueous layer was then 15 adjusted to 12.0 with 2N sodium hydroxide solution and the product was extracted with ethyl acetate. The ethyl acetate layer was dried over anhydrous sodium sulfate and concentrated under vacuum. The crude solid was washed with diethyl ether (50 mL) to obtain 8-bromo-2,3,4,5-tetrahydro- 1 H-pyrido [4,3 -b]indole (2.2g, 39.3%) with purity 98.82% by HPLC. 'HNMR (200 MHz, DMSO-d 6 ) 5: 2.7 (t, 2H, CH 2 ), 3.0 (t, 2H, CH 2 ), 3.8 20 (d, 2H, CH 2 ), 7.05 (d, 1H, Ar-H), 7.2 (d, 1H, Ar-H), 7.45 (s, 1H, Ar-H), 10.97 (bs, 1H, NH). m/e = 251 (M*+1). To a solution of 8-bromo-2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indole (250mg, 0.991 mmol) in anhydrous DMF (10 mL) was added Example 7 (258mg, 1.194 mmol) and potassium carbonate (204mg, 1.40 1mmol) at room temperature. The reaction mixture was 25 stirred at 100 0 C for 6 hours. After completion, the reaction mixture was diluted with water and the compound was extracted with EtOAc. The combined organic layers were dried over sodium sulfate, filtered and concentrated under reduced pressure. The residue obtained was 113 WO 2006/088949 PCT/US2006/005312 purified by column chromatography to obtain Example 33 (8Omg, 2 1%). HPLC: (RT =17.39min). 'H NMR (200 MHz, CDCl 3 ) ppm: 8.88 (s, 2H), 7.95 (bs, 1H), 7.65-7.15 (in, 3H), 5.05 (s, 2H), 4.35 (in, 2H), 3.88 (s, 3H), 2.93 (t, 2H, J= 5.4Hz); m/e = 387 (M+1). Examples 34 to 39 were prepared in the same manner as Example 15 by substituting 5 the appropriate phenyihydrazine for the 4-bromophenylhydrazine used in making Example 33 and processing the resulting ester to the hydroxamic acid as described for Example 12. 0 N '- NHOH CI N N N H Example 34. N-hydroxy-2-(7-chloro-8-methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3 10 b]indol-2-yl)pyrimidine-5-carboxamide Example 34 was obtained by substituting 3-chloro-4-methylphenyl hydrazine for 4 bromophenylhydrazine. A white solid (0.12g, 60%) of 94.6% purity by HPLC; 'HNMR (200 MHz, DMSO-D 6 ) 5: 2.39 (s, 3H, CH 3 ), 2.9 (t, 2H, CH 2 ), 4.2 (t, 2H, CH 2 ), 5.0 (s, 2H,

CH

2 ), 7.3 (s, 1H, Ar), 7.4 (s, 1H, Ar), 8.78 (s, 2H, Pyrimidine), 9.05 (bs, 1H, NH), 10.95 (s, 15 1H, NH), 11.1 (bs, 1H, OH). 0 N NHOH FQ N N Example 35. N-hydroxy-2-(7-fluoro-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxamide 20 Example 35 was obtained by substituting 3-fluorophenyl hydrazine for 4 bromophenylhydrazine. A solid (40 mg, 71.8%) with HPLC purity 94.97%; 'HNMR (200 MHz, DMSO-D 6 ) 8: 2.9 (t, 2H, CH 2 ); 4.2 (t, 2H, CH 2 ); 4.9 (s, 2H, CH 2 ); 6.8 (in, IH, Ar); 7.07 (dd, 1H, Ar); 7.4 (in, 1H, Ar); 8.7 (s, 2H, pyrimidine); 11.03 (s, IH, NH); m/e = 328 114 WO 2006/088949 PCT/US2006/005312 (M+1). 0 N NHOH N N Example 36. N-hydroxy-2-(7-methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-blindol-2 5 yl)pyrimidine-5-carboxamide Example 36 was obtained by substituting 3-methylphenyl hydrazine for 4 bromophenylhydrazine. A solid; 'IHNMR (200 MHz, DMSO-D 6 ) 8: 2.81 (t, 2H, CH 2 ); 4.2 (t, 2H, CH 2 ); 4.9 (s, 2H, CH 2 ); 6.8 (d, 1H, Ar); 7.15 (s, 1H, Ar); 7.3 (d, 1H, Ar); 8.7 (s, 2H, pyrimidine); 10.78 (s, 1H, NH); n/e = 324 (M+1). 10 0 0 2 N N NHOH - NIN N H Example 37. N-hydroxy-2-(8-nitro-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxamide Example 37 was obtained by substituting 4-nitrophenyl hydrazine for 4 15 bromophenylhydrazine. A greenish yellow colored solid (0.1g, 86.02%); 'HNMR (200 MHz, DMSO-d 6 ) 8: 2.9 (t, 2H, CH 2 ), 4.2 (t, 2H, CH 2 ), 5.1 (s, 2H, CH 2 ), 7.45 (d, 1H, Ar-H), 8.0 (d, 1H, Ar-H), 8.45 (s, 1H, Ar-H), 8.7 (s, 2H, Pyrimidine); n/e = 355.1 (M+1). 0 N NHOH NIN - |N N H 20 Example 38. N-hydroxy-2-{8-isopropyl-1,3,4,5-tetrahydro-2H-pyrido[4,3 115 WO 2006/088949 PCT/US2006/005312 b]indol-2-yl}pyrimidine-5-carboxamide Example 38 was obtained by substituting 4-isopropylphenyl hydrazine for 4 bromophenyihydrazine. A solid (0.045g, 56.25%), m/e = 352 (M+1). 0 N NHOH - N N N 5 H Example 39. N-hydroxy-2-{8-tert-butyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl}pyrimidine-5-carboxamide Example 39 was obtained by substituting 4-tert-butylphenyl hydrazine for 4 bromophenyihydrazine. A solid (0.2g, 86.9%), m/e = 366 (M+1). 10 Examples 111 and 112 were prepared in the same manner as Example 15 by substituting the appropriate phenylhydrazine for the 4-bromophenylhydrazine used in making Example 33 and processing the resulting ester to the hydroxamic acid as described for Example 12. 0 N ~ NHOH N 15 H Example 40. N-hydroxy-2-{8-[4-methoxyphenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 blindol-2-yl}pyrimidine-5-carboxamide Example 40 was obtained by substituting 4-methoxyphenylboronic acid for phenylboronic acid. White solid (0.07g, 53.7%) of 94.45% purity by HPLC; 'HNMR (200 20 MHz, DMSO-D 6 ) 8: 2.99 (t, 2H, CH 2 ), 3.8 (s, 3H, OCH 3 ), 4.22 (t, 2H, CH 2 ), 5.0 (s, 2H,

CH

2 ), 7.0 (d, 2H, Ar-H), 7.35 (d, 2H, Ar-H), 7.65 (d, 2H, Ar-H), 7.7 (s, 1H, Ar-), 8.7 (s, 2H, Pyrimidine-H), 9.0 (s, 1H, NH), 10.9 (s, 1H, NH), 11.1 (bs, 1H, OH); m/e = 416 (M+1). 116 WO 2006/088949 PCT/US2006/005312 0 N NHOH NIN N H Example 41. N-hydroxy-2-{8-[4-methylphenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl}pyrimidine-5-carboxamide 5 Example 41 was obtained by substituting 4-methylphenylboronic acid for phenylboronic acid. A white solid (17 mg, 33.9%) with 95.45% purity (by HPLC); 'HNMR (200 MHz, DMSO-D 6 ) 8: 2.3 (s, 3H, CH 3 ), 2.85 (t, 2H, CH 2 ), 4.24 (t, 2H, CH 2 ), 5.0 (s, 2H,

CH

2 ), 7.2 (d, 2H, Ar-H), 7.35 (d, 2H, Ar-H), 7.6 (d, 2H, Ar-H), 7.7 (s, 1H, Ar-H), 8.75 (s, 2H, Pyrimidine-H), 9.0 (bs, 1H, NH), 10.9 (s, 1H, NH), 11.1 (bs, 1H, NH); m/e = 400.0 10 (M+1). 0 0 N NHOH - NIN N H Example 42. N-hydroxy-2-{8-[3-(pyrrolidin-1-ylcarbonyl)phenyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide 15 Example 42 was obtained by substituting 3-(pyrrolidin-1-ylcarbonyl)phenylboronic acid for phenylboronic acid. A white solid 85 mg (56.6%) of 97.25% pure by HPLC; 1 HNMR (200 MHz, CD 3 0D) 8: 2.0 (m, 4H, 2 x CH 2 ), 2.99 (t, 2H, CH 2 ), 3.5-3.7 (m, 4H, 2 x

CH

2 ), 4.4 (t, 2H, CH 2 ), 5.1 (s, 2H, CH 2 ), 7.4-7.85 (m, 7H, Ar-H), 8.7 (s, 2H, Pyrimidine-H); m/e = 484 (M+1). 20 117 WO 2006/088949 PCT/US2006/005312

HOH

2 C 0 N NHOH - N N N H Example 43. N-hydroxy-2-{8-[3-(hydroxymethyl)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-blindol-2-yl}pyrimidine-5-carboxamide Example 43 was obtained by substituting 3-(hydroxymethyl)phenylboronic acid for 5 phenylboronic acid. A white solid (0.025g, 41.66%) of 91.09% purity by HPLC; IHNMR (200 MHz, CD 3 0D) [: 2.99 (t, 2H, CH 2 ), 4.4 (t, 2H, CH 2 ), 4.7 (s, 2H, CH 2 ), 5.18 (s, 2H,

CH

2 ), 7.2-7.75 (m, 7H, Ar-H), 8.88 (s, 2H, Pyrimidine-H); m/e = 416 (M+1). F 0 N ' NHOH -__ N N N H 10 Example 44. N-hydroxy-2-{8-[3-fluorophenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl}pyrimidine-5-carboxamide Example 44 was obtained by substituting 3-fluorophenylboronic acid for phenylboronic acid. A pale yellow colored solid (.013g, 21.6%) of 94.45% purity by HPLC; 'HNMR (200 MHz, DMSO-d 6 ) 5: 2.9 (t, 2H, CH 2 ), 4.25 (t, 2H, CH 2 ), 5.0 (s, 2H, 15 CH 2 ), 7.0 (t, 1H, Ar-H), 7.2-7.6 (m, 5H, Ar-H), 7.8 (s, 1H, Ar-H), 8.7 (s, 2H, Pyrimidine), 9.0 (bs, 1H, NH); m/e = 404 (M+1). F 0 N NHOH NIN' __ N N N H Example 45. N-hydroxy-2-{8-[4-fluorophenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 118 WO 2006/088949 PCT/US2006/005312 blindol-2-yl}pyrimidine-5-carboxamide Example 45 was obtained by substituting 4-fluorophenylboronic acid for phenylboronic acid. A pale yellow colored solid (0.01g, 25%) of 99.23% purity by HPLC; 'HNMR (200 MHz, CD 3 OD) 6: 2.9 (t, 2H, CH 2 ), 4.25 (t, 2H, CH 2 ), 5.1 (s, 2H, CH 2 ), 7.0 5 7.2 (t, 2H, Ar-H), 7.35 (t, 2H, Ar-H), 7.6 (m, 3H, Ar-H), 8.7 (s, 2H, Pyrimidine); m/e = 404 (M+1). 0 N NHOH N N N H Example 46. N-hydroxy-2-{8-(fur-2-yl)-1,3,4,5-tetrahydro-2H-pyrido[4,3-bjindol-2 10 yl}pyrimidine-5-carboxamide Example 46 was obtained by substituting fur-2-ylboronic acid for phenylboronic acid. A solid (1.08g); HPLC:(RT-14.2); m/e 376 (M+1). N\/ N ~ NHOH N N N H 15 Example 47. N-hydroxy-2-{8-(pyridin-3-yl)-1,3,4,5-tetrahydro-2H-pyrido[4,3-blindol 2-yl}pyrimidine-5-carboxamide Example 47 was obtained by substituting pyridin-3-ylboronic acid for phenylboronic acid. A solid (0.017g, 23.93%); HPLC: 91.59% (RT = 11.31); 'IHNMR (CD 3 0D, 200 MHz) 6: 8.72 (s, 2H), 8.82 (s, 1H), 8.41 (d, 1H), 8.06 (d, 1H),7.412-7.77 (m, 5H), 5.09 (s, 20 2H), 4.24 (m, 2H), 2.84 (m, 2H); m/e = 386 (M+1). 119 WO 2006/088949 PCT/US2006/005312 -N 0 N NHOH N N NC H Example 48. N-hydroxy-2-{8-[4-dimethylaminophenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-blindol-2-yl}pyrimidine-5-carboxamide Example 48 was obtained by substituting 4-(dimethylamino)phenylboronic acid. A 5 whtie solid (20 mg, 83%); 'H NMR (300 MHz, DMSO-d 6 ): 2.89 (m, 2H), 3.30 (s, 6H), 4.25 (m, 2H), 5.01 (s, 2H), 7.11 (m, 2H), 7.32 (s, 1H), 7.64-7.70 (m, 3H), 8.71 (s, 2H), 10.94 (s, 1H); m/e = 429 (M+1). /S \ / 0 N NHOH N H 10 Example 49. N-hydroxy-2-{8-[3-(methylthio)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide Example 49 was obtained by substituting 3-(methylthio)phenylboronic acid for phenylboronic. A solid (0.065g, 86%); HPLC; 97.97% (RT-15.32); 'HNMR (DMSO-D 6 , 200 MHz) 8: 11.01 (1H, s), 8.77 (2H, s), 7.80 (1H, s), 7.54-7.15 (Ar, 7H, m), 5.01 (2H, s), 15 4.23 (2H, m), 3.33 (3H, s), 2.88 (2H, m); m/e = 432 (M+1). H O N 0 NN NHOH NIN' N H 120 WO 2006/088949 PCT/US2006/005312 Example 50. N-hydroxy-2-{8-[4-(N-acetamido)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-blindol-2-yl}pyrimidine-5-carboxamide Example 50 was obtained by substituting 4-(N-acetamido)phenylboronic acid for 5 phenylboronic acid. A solid (0.030g, 35.2%); HPLC: 88.28% (RT-12.75); 'IHNMR

(DMSO-D

6 , 200 MHz) 5: 10.97 (1H, s), 9.96 (1H, s), 8.72 (2H, s), 7.73-7.33 (Ar, 7H, m), 5.01 (2H, s), 4.24 (2H, m), 2.80 (2H, m), 2.05 (3H, s); m/e = 443 (M+1). N N O-N N ~ NHOH SN N N N' H 10 Example 51. N-hydroxy-2-{8-(pyrimidin-5-yl)-1,3,4,5-tetrahydro-2H-pyrido[4,3 blindol-2-yl}pyrimidine-5-carboxamide Example 51 was obtained by substituting pyrimidin-5-ylboronic acid for phenylboronic acid. A solid (0.025g, 35.61%); HPLC: 94.4128% (RT-11.98); 'HNMR

(CD

3 0D, 200 MHz) 6: 9.20 (1H, s), 9.15 (2H, s), 8.81 (2H, s), 7.96 (1H, s), 7.14 (2H, d), 15 5.18 (2H, s), 4.39 (2H, m), 2.82 (2H, m); m/e = 388 (M+1). 0 \ / 0 N NHOH ~ N N N H Example 52. N-hydroxy-2-{8-[fur-3-yl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl}pyrimidine-5-carboxamide 20 Example 52 was obtained by substituting fur-3-ylboronic acid for phenylboronic acid. A solid; (0.1 16g). 'HNMR (DMSO-D 6 , 200 MHz) 5: 10.93 (1H, s), 8.75 (2H, s), 8.09 (1H, s), 7.60 (2H, d), 7.28 (2H, s), 7.00 (1H, s), 4.99 (2H, s), 4.24 (2H, m), 2.87 (2H, m); m/e = 376 (M+1). 121 WO 2006/088949 PCT/US2006/005312 0 HN N N " NHOH - N N N H Example 53. N-hydroxy-2-{8-[1H-pyrrol-2-yl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl}pyrimidine-5-carboxamide 5 Example 53 was obtained by substituting 1H-pyrrol-2-ylboronic acid for phenylboronic acid. A solid (0.015 g, 35%); 'H NMR (200 MHz, CD 3 0D): 8 2.89 (m, 2H), 4.40 (m, 2H), 5.15 (m, 2H), 6.1 (m, 2H), 6.8 (s, 1H), 7.20 (s, 1,H), 7.40 (m, 2H), 8.66 (s, 2H); MS: 375 (M+1). Examples 54 to 57 were prepared in the same manner as Example 15 by substituting 10 the appropriate phenylhydrazine for the 4-bromophenylhydrazine used in making Example 33 and processing the resulting ester to the hydroxamic acid as described for Example 12. 0 CN NHOH N IN) N H Example 54. N-hydroxy-2-(9-chloro-8-methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3 15 b]indol-2-yl)pyrimidine-5-carboxamide Example 54, an isomer of Example 34, was also obtained by substituting 3-chloro-4 methylphenyl hydrazine for 4-bromophenylhydrazine. A white solid (0.03 g, 60%); 'H NMR (200 MHz, CD 3 0D): 8 2.35(s, 3H), 2.89 (m, 2H), 4.40 (m, 2H), 5.15 (m, 2H), 7.40(m, 2H), 8.66 (s, 2H); m/e = 358 (M+1). 20 122 WO 2006/088949 PCT/US2006/005312 0

F

3 C N NHOH I NIN N H Example 55. N-hydroxy-2-(8-trifluoromethyl-1,3,4,5-tetrahydro-2H-pyrido[4,3 blindol-2-yl)pyrimidine-5-carboxamide Example 55 was obtained by substituting 4-(trifluoromethyl)phenyl hydrazine for 4 5 bromophenylhydrazine. A solid (0.07 g, 70%); 'H NMR (200 MHz, CD 3 0D): 5 2.89 (in, 2H), 4.40 (m, 2H), 5.15 (m, 2H), 7.40(m, 2H), 7.80(s, 1H), 8.66 (s, 2H); m/e = 378 (M+1). 0 N -' NHOH NIN N H Example 56. N-hydroxy-2-(7,8-dimethyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-blindol-2 10 yl)pyrimidine-5-carboxamide Example 56 was obtained by substituting 3,4-dimethylphenyl hydrazine for 4 bromophenylhydrazine. A solid, (0.071gm), m/e = 338 (M+1). 0 MeO N NHOH N N N H 15 Example 57. N-hydroxy-2-(8-methoxy-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxamide Example 57 was obtained by substituting 4-methoxyphenyl hydrazine for 4 bromophenyihydrazine in the reaction sequence. A white solid; 'H NMR (300 MHz,

DMSO-D

6 ) 8: 2.83 (m, 2H), 3.74 (s, 3H), 4.21 (m, 2H), 4.92 (s, 2H), 8.65 (m, 1), 6.97 (d, J 20 =2.4, 1H), 7.14 (d, J= 8.7, 1H), 8.70 (S, 2H), 10.23 (s, 2H), 10.74 (s, 1H); m/e = 340 123 WO 2006/088949 PCT/US2006/005312 (M+1). HO 0 >N N NHOH N NN Example 58. N-hydroxy-2-[9-(2-hydroxyethyl)-1,3,4,9-tetrahydro-2H-b-carbolin-2 5 yllpyrimidine-5-carboxamide To a stirred solution of Example 8 (0.1 gm,0.32mmoles) in DMF at 0 0 C under nitrogen atmosphere was added NaH (0.025gm, 1.04 mmol) and stirred for 30 minutes. To the reaction mixture was added O-(tert-butyl-dimethylsilyl)-2-bromoethanol (0.077gm, 0.32 mmol), the cooling was removed and the reaction stirred for 3hr. The progress of the 10 reaction was monitored by tic. Upon completion of the reaction, the reaction mixture was partitioned between ethyl acetate and water. The organic layer was separated and dried over sodium sulphate, filtered and the solvent was removed under reduced pressure to provide an intermediate acid (0.07gm). To a stirred solution of the acid (0.07gm, 0.15 mmol) in DCM (15 mL) at 0-5 'C 15 was added HOBt (013gm, 0.23 mmol), EDC (0.043gm, 0.23 mmol), DIEA (0.065gm, 0.53 nimol) and NH 2 OTHP (0.0 17gm, 0.15 mmol) and the reaction was stirred over night. The progress of the reaction was monitored by TLC and upon completion of the reaction, the reaction mixture was partitioned between DCM and water. The organic layer was separated, dried over sodium sulphate, filtered and the solvent was removed under reduced pressure to 20 provide the protected hydroxamic acid (0.025gm). To a stirred solution of the protected hydroxamic acid at 0 C (.025gm, 0.04mmol) in methanol (3mL) was added ether-HCl (5 mL). After 15 minutes, the progress of the reaction was monitored by tlc and upon completion of the reaction, the solvent was removed under reduced pressure at 40 "C and the residue purified by ether washings to obtain 25 Example 58 (0.011 gm). IHNMR (CD 3 0D, 200 MHz) 5: 8.75 (2H, s), 7.63-7.02 (5H, in), 5.01 (2H, s), 4.31 (4H, in), 3.87 (4H, in), 2.88 (2H, in); m/e = 354 (M+1). 124 WO 2006/088949 PCT/US2006/005312 N/ O N NHOH N NAN Example 59. N-hydroxy-2-[9-(1-methylpiperidin-3-yl)-1,3,4,9-tetrahydro-2H-b carbolin-2-yljpyrimidine-5-carboxamide 5 To a stirred solution of Example 8 (500 mg, 1.62 mmol) in THF (5 mL) and water (5 mL) was added lithium hydroxide (205 mg, 8.54 mmol) at room temperature and the mixture was stirred for 4-5 h. The reaction was monitored by TLC and after completion, the reaction mixture was acidified using IN hydrochloric acid. The mixture was extracted with ethyl acetate, washed with brine, dried over sodium sulfate and concentrated to obtain the 10 carboxylic acid (400 mg, 83%). To a stirred solution of the carboxylic acid (400 mg, 1.36 mmol) in DCM was added EDCI (390 mg, 2.0 mmol) at 00 C. The mixture was stirred at 0 0 C for 15 min and then

NH

2 OTHP (160 mg, 1.36 mmol) was added. The reaction mixture stirred overnight. After completion of the reaction it was diluted with DCM. The DCM layer was separated, washed 15 with saturated sodium bicarbonate, water and concentrated. The crude product obtained was purified by silica gel column chromatography to obtain the protected hydroxamic acid (250 mg, 46.8%), m/e = 394 (M*+1) To a stirred solution of the protected hydroxamic acid (262 mg, 0.66 mmol) in DMF (5 mL) was added sodium hydride (60 mg, 2.5 mmol) and the mixture was stirred at room 20 temperature for 20 min. 3-Bromo-N-methylpiperadine (600 mg, 3.3 mmol) was added and the reaction mixture was allowed to stir at room temperature for 12 h. After removing DMF, water was added and the mixture extracted with DCM. The pure alkylated product (35 mg, 9%) was obtained by purifying through silica gel column chromatography using 20%methanol in DCM (100 mg, 31%); m/e = 477 (M+1) 25 The alkylated product (30 mg, 0.06 mmol) was stirred in 30% TFA in DCM (10 mL) for 25-30 min at room temperature. After removing the solvents the solid that separated 125 WO 2006/088949 PCT/US2006/005312 out was filtered, washed with ether and dried under vacuum to get Example 59 (20 mg, 83%) as a white solid; m/e = 407 (M+1). 0 N NHOH _ NIN) N H 5 Example 60. N-hydroxy-2-{8-[thien-2-yl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl}pyrimidine-5-carboxamide To a solution of Example 33 (0.25g, 0.64 mmol) in dimethylacetamide (5 mL) were added thiophene (0.5 mL) and potassium acetate (0.126g, 1.28 mmol) and the reaction mixture was thoroughly degassed and freshly prepared tetrakistriphenylphosphine 10 palladium(0) (0.06g, 0.052mmoles) was added under nitrogen atmosphere at room temperature. The reaction mixture temperature was raised to 80 0 C and continued stirring for 12 hr in a sealed tube. The progress of the reaction was monitored by TLC and upon completion of the reaction the solvent was evaporated under reduced pressure. The mixture was partitioned between ethyl acetate (100 mL) and water (15 mL) and the organic layer 15 was separated, dried over Na 2

SO

4 filtered and the solvent was removed under reduced pressure to give crude residue. Purification by column chromatography using silica gel provided an intermediate ester (0.048g, 19.06%). HPLC: 76.71 (Rt 16.35 min). To a 0 "C solution of the intermediate ester (0.04g, 0.10 mmol) in methanol: DCM (5:2 mL) was added 50% aqueous NH20H solution (lmL) and to the mixture was added 20 solution of NaOH (0.040g) in water (0.2mL). The reaction mixture was stirred at room temperature for lhr and the progress of the reaction was monitored by TLC. Upon completion of the reaction the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using 1N hydrochloric acid and the obtained solid was filtered and washed with water followed by diethyl ether. Drying under vacuum gave Example 60 25 (0.008 g, 20%). HPLC: 85.67% (Rt-13.09); 'HNMR (DMSO-D 6 , 200 MHz) 6: 10.90 (1H, s), 8.71 (2H, s), 6.95 (2H, in), 7.46-7.02 (Ar, 6H, in), 4.99 (2H, s), 4.23 (in, 2H), 2.86 (2H, in); m/e = 392 (M+1). 126 WO 2006/088949 PCT/US2006/005312 Examples 61-65 were prepared in the same manner as Example 10 by substituting the appropriate 2,3,4,9-tetrahydro- 1 H-b-carboline for 6-methoxy-2,3,4,9-tetrahydro- 1 H-b carboline. 0 H N NHOH H N N N 5 Example 61. N-hydroxy-2-(8-methyl-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxamide Example 61 was obtained by substituting 8-methyl-2,3,4,9-tetrahydro-1H-b carboline for 6-methoxy-2,3,4,9-tetrahydro-1H-b-carboline. A solid (0.008gm); 'HNMR

(CD

3 0D, 200 MHz) 8: 8.73 (2H, s), 7.24 (1H, d), 6.86 (2H, m), 4.90 (2H, s), 4.30 (2H, m) 10 2.85 (2H, m), 2.48 (3H, s); m/e = 324 (M+1). 0 H N NHOH N N N Example 62. N-hydroxy-2-(7-fluoro-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxamide 15 Example 62 was obtained by substituting 7-fluoro-2,3,4,9-tetrahydro-1H-b-carboline for 6-methoxy-2,3,4,9-tetrahydro-1H-b-carboline. A solid (0.027 g, 67%); 1 H NMR (200 MHz, CD 3 0D): 8 2.89 (m, 2H), 4.40 (m, 2H), 5.15 (m, 2H), 6.57(s, 1H), 7.36(m, 2H), 8.66 (s, 2H); m/e = 328 (M+1). 127 WO 2006/088949 PCT/US2006/005312 0 NHOH H I N N N F Example 63. N-hydroxy-2-(6-fluoro-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxamide Example 63 was obtained by substituting 6-fluoro-2,3,4,9-tetrahydro-1H-b-carboline 5 for 6-methoxy-2,3,4,9-tetrahydro-1H-b-carboline. A solid (25 mg); 'HNMR (200 MHz,

CD

3 0D) 8: 2.93 (t, 2H, CH 2 ), 4.28 (t, 2H, CH 2 ), 5.1 (s, 2H, CH 2 ), 6.89 (t, 1H, Ar-H), 7.1 (d, 1H, Ar-H), 7.3 (m, 1H, Ar-H), 8.82 (s, 2H, Pyrimidine ring-H); m/e = 328 (M + 1). 0 N NHOH HI N N N' '0 10 Example 64. N-hydroxy-2-(6-benzyloxy-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxamide Example 64 was obtained by substituting 6-benzyloxy-2,3,4,9-tetrahydro-1H-b carboline for 6-methoxy-2,3,4,9-tetrahydro-1H-b-carboline. A solid (0.075gm); 'HNMR 15 (CD 3 0D, 200 MHz) 5: 8.82 (2H, s), 7.35-6.90 (Ar, 8H, m), 5.20 (2H, s), 5.01 (2H, s), 4.24 (2H, m), 2.80 (2H, m); m/e = 416 (M+1). 0 NN~ NHOH H N 2 N 128 WO 2006/088949 PCT/US2006/005312 Example 65. N-hydroxy-2-(7-methyl-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxamide Example 65 was obtained by substituting 7-methyl-2,3,4,9-tetrahydro-1H-b carboline for 6-methoxy-2,3,4,9-tetrahydro-1H-b-carboline. A solid (0.02g, 22.13%); 5 HPLC: 80.03% (Rt-14.11). 'HNMR (CD 3 0D, 200 MHz) 8: 8.73 (2H, s), 6.82-7.38 (Ar-3H, in), 5.2 (2H, s), 4.30 (2H, t), 2.80 (2H, in), 2.42 (3H,s); m/e = 324 (M+1). 0 H ~

OCH

3 N N N Example 66. Methyl 2-(6-methyl-1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5 10 carboxylate To a stirred solution of 2-(5-methyl-1H-indol-3-yl)ethanamine (0.54g, 2.56 mmol) in water was added (5 mL) glyoxalic acid (228 mg, 3.08mmol) and the reaction stirred for 10 min. A potassium hydroxide solution (140 mg in 5 mL) was added and stirred at room temperature for Ih. The progress of the reaction was monitored by TLC and after 15 completion, the solid material obtained was filtered. The wet cake was suspended in water (5 mL), conc. hydrochloric acid (1 mL) was added and the mixture heated to 60-700 C for 0.5 h. Additional conc. HCl (1 mL) was added and the solvent evaporated by heating on a hot plate. The dry solid was suspended in water (10 mL) and heated to 45-550 C and the pH was adjusted to 12 with 20% KOH solution. The solid product obtained was filtered and 20 dried to give the tetrahydrocarboline (200 mg, 37%). 'HNMR (200 MHz, CDCl 3 ) 8: 2.45 (s, 3H, CH 3 ), 2.75 (t, 2H, CH 2 ), 3.2 (t, 2H, CH 2 ), 4.1 (s, 2H, CH 2 ), 6.99 (d, 1H, Ar-H), 7.1 (s, 1H, Ar-H), 7.2 (d, 1H, Ar-H). To a stirred solution of the tetrahydrocarboline (0.15g, 0.80 mmol) in DMF (10 mL) were added Example 7 (0.226g, 1.0462 mmol) and potassium carbonate (0.278g, 2.01 25 mmol) The mixture was heated to 80-900 C for 2-3h. The progress of the reaction was followed by TLC and after completion DMF was distilled off under vacuum. The residue 129 WO 2006/088949 PCT/US2006/005312 was partitioned between ethyl acetate (100 mL) and water and the two layers were separated. The aqueous layer was extracted with ethyl acetate (100 mL) and both the organic layers were combined. The ethyl acetate layer was dried over anhydrous sodium sulfate, concentrated under vacuum and the residue was purified by washing with diethyl 5 ether (100 mL) to give Example 66 (100 mg, 3 8.5%). 'HNMR (200 MHz, CDC1 3 ) 8: 2.4(s, 3H, CH 3 ), 2.8 (t, 2H, CH 2 ), 3.8 (s, 3H, CH 3 ), 4.3 (t, 2H, CH 2 ), 5.1 (s, 2H, CH 2 ), 7.0 (d, 1H, Ar-H), 7.18 (s, 1H, Ar-H), 7.2 (d, 1H, Ar-H), 7.79(bs,1H, NH), 8.8 (s,2H,CH 2 ). 0 N NHOH H NN 10 Example 67. N-hydroxy-2-(6-methyl-1,3,4,4a,9,9a-hexahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxamide To a stirred solution of Example 66 (150 mg, 0.46 mmol) in dry DCM was added TFA (8.88g, 77.89 mmol) and the mixtue stirred for 30 min. Triethylsilane (4.368g, 37.56 mmol) was added at room temperature and stirred for 2h. The progress of the reaction mass 15 was monitored by TLC and after completion, the reaction was diluted with DCM (20 mL) and washed with saturated solution of sodium bicarbonate (20 mL), then with water. The organic layer was dried over sodium sulfate and concentrated. The residue was purified by column chromatography to give the reduced tetrahydrocarboline (55 mg, 36%); m/e = 325 (M+1). 20 To a stirred solution of the reduced tetrahydrocarboline (50 mg, 0.15 mmol) in methanol (3 mL) and DCM (2 mL) was added 50% aqueous hydroxylamine hydrochloride (1 mL) and sodium hydroxide solution (40 mg mmol) in 0.25 mL of water at 0 0 C and the mixture was stirred at 0 0 C for 10 min. The reaction was then allowed to come to room temperature and maintained at room temperature for 1h. The progress of the reaction was 25 monitored by TLC and after completion, the solvents were removed under vacuum. The mixture was diluted with water (10 mL) and neutralized with 2N hydrochloric acid (pH 6.5 130 WO 2006/088949 PCT/US2006/005312 7.0). The white solid that separated out was filtered and dried to give Example 67 (32 mg, 63.8%) with HPLC purity of 98.5%. 'HNMR (200 MHz, DMSO-d 6 ) 6: 1.8 (m, 1H), 2.0 (m, 1H), 2.2 (s, 3H, CH 3 ), 3.45-4.0 (m, 6H), 5.4 (s, 1H), 6.4 (d, 1H, Ar-H), 6.7 (d, 1H, Ar-H), 6.9 (s, 1H, Ar-H), 8.65 (s, 2H, Pyrimidine), 9.0 (bs, 1H, NH), 11.0 (bs, 1H, NH); m/e = 326 5 (M+I). 0 N N N HO Example 68. N-hydroxy-2-(6-methyl-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl)pyrimidine-5-carboxamide 10 To a stirred solution of Example 66 (70 mg, 0.21 nmol) in methanol (5 mL) and DCM (3 mL), 50% aqueous hydroxylamine hydrochloride (1.5 mL) and sodium hydroxide solution (60 mg 1.5 mmol) in 0.5 mL of water were added at 0 0 C and the mixture was stirred at 0 0 C for 10 min. The reaction was then allowed to come to room temperature and maintained at room temperature for lh. The progress of the reaction was monitored by TLC 15 and after completion; the solvents were removed under vacuum. The mixture was diluted with water (10 mL) and neutralized with 2N hydrochloric acid (pH 6.5-7.0). The white product separated out, was filtered and dried to give Example 68 (50 mg, 71.4%) with HPLC purity of 96.91%. 'HNMR (200 MHz, DMSO-d 6 ) 6: 2.4 (s, 3H, CH 3 ), 2.78 (t, 2H,

CH

2 ), 4.2 (t, 2H, CH 2 ), 5.0 (s, 2H, CH 2 ), 6.82 (d, 1H, Ar-H), 7.18 (s, 1H, Ar-H), 7.2 (d, 1H, 20 Ar-H), 8.75 (s, 2H, Pyrimidine-H), 10.78 (bs, 1H, NH); m/e = 324 (M+I). 0 N N

OCH

3 N \ Br 131 WO 2006/088949 PCT/US2006/005312 Example 69. Methyl 2-(6-bromo-1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5 carboxylate To a stirred solution of 2-(5-bromo-lH-indol-3-yl)ethanamine (1.0g, 3.64 mmol) in methanol: toluene (1:1, 20 mL) was added paraformaldehyde (200 mg, 20%). The reaction 5 mass was refluxed for 24h. The progress of the reaction was monitored by TLC and after completion, the reaction mass was concentrated and the crude was washed with ethyl acetate. The aqueous layer was adjusted to pH=12 with 20% KOH solution. The precipitated solid was extracted with ethyl acetate and the organic layer washed with brine solution and concentrated to give the tetrahydrocarboline (350 mg, 38.39%). 'HNMR (200 10 MHz, DMSO-d 6 ) 8: 2.65 (t, 2H, CH 2 ), 3.0 (t, 2H, CH 2 ), 3.92 (s, 2H, CH 2 ), 7.1 (d, 1H, Ar H), 7.21 (d, 1H, Ar-H), 7.45 (s, 1H, Ar-H), 10.95 (s, 1H, NH); m/e = 251 (M+1). To a stirred solution of the tetrahydrocarboline (350 mg, 1.4 nmol) in DMF (20 mL) were added Example 7 (453 mg, 2.09 mmol) and potassium carbonate (580 mg, 4.20 mmol) and the mixture was heated to 80-900 C for 2-3h. The progress of the reaction was followed 15 by TLC and after completion DMF was distilled off under vacuum. The residue was partitioned between ethyl acetate (150 mL) and water and the two layers were separated. The aqueous layer was extracted with ethyl acetate (150 mL) and both the organic layers were combined. The ethyl acetate layer was dried over anhydrous sodium sulfate, concentrated under vacuum and the residue was purified by washing with diethyl ether (100 20 mL) to give Example 69 (250 mg, 46.31%). 'HNMR (200 MHz, DMSO-d 6 ) a: 2.93 (t, 2H,

CH

2 ), 3.8 (s, 3H, OCH 3 ), 4.28 (t, 2H, CH 2 ), 5.0 (s, 2H, CH 2 ), 7.18 (d, 1H, Ar-H), 7.22 (d, 1H, Ar-H), 7.7 (s, 1H, Ar-H), 8.82 (s, 2H, Pyrimidine-H), 11.2 (s, 1H N-H); m/e = 387(M+1). 0 N NHOH H N N . 0 N ~ 25 132 WO 2006/088949 PCT/US2006/005312 Example 70. N-hydroxy-2-{6-[3-(morpholin-4-ylmethyl)phenyl]-1,3,4,9-tetrahydro 2H-b-carbolin-2-yl}pyrimidine-5-carboxamide To a stirred solution of Example 69 (420 mg, 1.08 mmol) and 3-formylphenyl 5 boronic acid (320 mg, 2.172 mmol) in THF/water (1:1, 20 mL) was added tetrakistriphenylphosphinepalladium(0) (126 mg, 0.10 mmol) and potassium carbonate (900 mg, 6.32 mmol) and the resulting mixture was heated to reflux for 5-6h. The progress of the reaction was monitored by TLC and after completion the reaction was cooled to room temperature. The mixture was diluted with ethyl acetate (100 mL) and the two layers were 10 separated. The organic layer was washed with water, dried over anhydrous sodium sulfate and concentrated in vacuum. The residue was purified by column chromatography using silica gel to give the coupling product (200 mg, 44.64%). 'HNMR (200 MHz, CD 3 0D) 8: 3.0 (t, 2H, CH 2 ), 3.9 (s, 3H, OCH 3 ), 4.42 (t, 2H, CH 2 ), 5.25 (s, 2H, CH 2 ), 7.42 (s, 1H, Ar H), 7.5-7.8 (in, 6H, Ar-H), 8.21 (bs, 1H, N-H), 8.9 (s, 2H, Pyrimidine-H), 10.1 (s, 1H, 15 CHO); m/e = 413 (M+1). To a stirred solution of the coupling product (200 mg, 0.48 mmol) in dry DCM (10 mL) were added morpholine (423 mg, 4.85 mmol) and sodium triacetoxyborohydride (512 mg, 2.42 mmol) at room temperature and the mixture was stirred at the same temperature overnight. After completion, the reaction was diluted with DCM (40 mL) and washed with 20 saturated sodium bicarbonate solution and water. The organic layer was dried over anhydrous sodium sulfate and concentrated in vacuum. The residue was purified by column chromatography to give the reductive alkylation product (160 mg, 68.37%). 'HNMR (200 MHz, CDCl 3 ) 8: 2.58 (t, 4H, 2 x CH 2 ), 3.0 (t, 2H, CH 2 ), 3.62 (s, 2H, CH 2 ), 3.75 (t, 4H, 2 x

CH

2 ),-3.9 (s, 3H, OCH 3 ), 4.41 (t, 2H, CH 2 ), 5.18 (s, 2H, CH 2 ), 7.2-7.5 (in, 3H, Ar-H), 7.5 25 7.7 (ma 2H, Ar-H), 7.8 (s, 1H, Ar-H), 7.92 (s, 1H, Ar-H), 8.95 (s, 2H, pyrimidine-H); m/e = 484 (M+ 1). To a stirred solution of the reductive alkylation product (150 mg, 0.31 mmol) in methanol (8 mL) and DCM (4 mL), 50% aqueous hydroxylamine hydrochloride (5 mL, 35.97 mmol) and sodium hydroxide solution (120 mg 3.0 mmol in 0.5 mL of water) were 30 added at 0 0 C and the mixture was stirred at 0 0 C for 10 min. The reaction was then allowed 133 WO 2006/088949 PCT/US2006/005312 to come to room temperature and maintained at room temperature for lh. The progress of the reaction was monitored by TLC and after completion the solvents were removed under vacuum. The mixture was diluted with water (10 mL) and neutralized with 2N hydrochloric acid (pH 6.5-7.0). The white product separated out, was filtered and dried to give Example 5 70 (100 mg, 88.95%) of 94.5% purity by HPLC. 'HNMR (200 MHz, CD 3 0D) 8: 2.6 (t, 4H,

CH

2 ), 2.9 (t, 2H, CH 2 ), 3.61 (s, 2H, CH 2 ), 3.75 (t, 4H, 2 x CH 2 ), 4.25 (t, 2H, CH 2 ), 5.10 (s, 2H, CH 2 ), 7.21 (d, 1H, Ar-H), 7.32-7.65 (m, 6H, Ar-H), 8.78 (s, 2H, Pyrimidine-H); m/e = 485 (M+1). 0 NHOH HV N N N N N N ~~ 10 Example 71. N-hydroxy-2-{6-[3-((4-methylpiperazin-1-yl)methyl)phenyl]-1,3,4,9 tetrahydro-2H-b-carbolin-2-yl}pyrimidine-5-carboxamide Example 71 was prepared in the same manner as Example 70 by substituting 1 15 methylpiperazine for morpholine in the reductive alkylation reaction. A white solid (0.05g, 50%) of 98.2% purity by HPLC; 'HNMR (200 MHz, CD 3 0D) 8: 2.3 (s, 3H, CH 3 ), (m, 8H, 4 x CH 2 ), 2.95 (t, 2H, CH 2 ), 3.65 (s, 2H, CH 2 ), 4.36 (t, 2H, CH 2 ), 5.15 (s, 2H, CH 2 ), 7.2-7.8 (m, 7H, Ar-H), 8.8 (s, 2H, Pyrimidine); m/e = 497.8 (M+ 1). 134 WO 2006/088949 PCT/US2006/005312 0 NHOH H N N N NHO N

---

N \ Example 72. N-hydroxy-2-{6-[4-((4-methylpiperazin-1-yl)methyl)phenyl]-1,3,4,9 tetrahydro-2H-b-carbolin-2-yl}pyrimidine-5-carboxamide Example 72 was prepared in the same manner as Example 71 by substituting 4 5 formylphenyl boronic acid for 3-formylphenyl boronic acid in the palladium catalyzed coupling reaction with Example 69. A white solid (0.110g, 73%) of 96.5% purity by HPLC. 'HNMR (200 MHz, CD 3 0D) 6: 2.35 (s, 3H, CH 3 ), 2.6 (m, 8H, 4 x CH 2 ), 2.97 (t, 2H, CH 2 ), 3.60 (s, 2H, CH 2 ), 4.35 (t, 2H, CH 2 ), 5.05 (s, 2H, CH 2 ), 7.32-7.8 (m, 7H, Ar-H), 8.78 (s, 2H, Pyrimidine-H); m/e = 498 (M+ 1). 10 0 N N NHOH N N Example 73. N-hydroxy-2-{6-[2-((4-methylpiperazin-1-yl)methyl)phenyl]-1,3,4,9 tetrahydro-2H-b-carbolin-2-yl}pyrimidine-5-carboxamide Example 73 was prepared in the same manner as Example 71 by substituting 2 15 formylphenyl boronic acid for 3-formylphenyl boronic acid in the palladium catalyzed coupling reaction with Example 69. A white solid (92 mg, 45%) of 96.1% purity by HPLC. 'HNMR (200 MHz, CD 3 OD) 6: 2.28 (s, 3H, CH 3 ), 2.45 (m, 8H, 4 x CH 2 ) 2.92 (t, 2H, CH 2 ), 135 WO 2006/088949 PCT/US2006/005312 3.55 (s, 2H, CH 2 ), 4.38 (t, 2H, CH 2 ), 5.09(s, 2H, CH 2 ), 7.05 (d, 1H, Ar-H), 7.25-7.65 (in, 6H, Ar-H), 8.78 (s, 2H, Pyrimidine-H); m/e = 498 (M+1). 0

H

3 C N NHOH NIN N 5 Example 74. N-hydroxy-2-{5-acetyl-8-methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3 blindol-2-yl}pyrimidine-5-carboxamide From 8-methyl-2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indole and Example 7 was obtained methyl-2-(8-methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5 carboxylate using the method described in Example 12. To a solution of methyl-2-(8 10 methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5-carboxylate (110 mg) in DMF (5 mL) was added sodium hydride (22 mg) at 0 C and the mixtue was stirred for 20 min. Acetyl chloride (30 mg) was added and the reaction was allowed to attain room temperature and stirred another 3 hours. The reaction mixtue was partitioned between ethyl acetate and water. The organic layer was separated and washed with water and saturated 15 aqueous sodium bicarbonate. The solvent was dried and evaporated and the residue purified by chromatography to give methyl-2-(5-acetyl-8-methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl)pyrimidine-5-carboxylate. To a cooled solution of hydroxylamine hydrochloride (0.043 g, 0.618 mmol) in DCM (3 mL) was added trimethylaluminium (0.135 g, 1.87 mmol) at 0 0 C. The reaction 20 mixture was warmed to Rt for lh and methyl-2-(5-acetyl-8-methyl-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl)pyrimidine-5-carboxylate (0.050 g, 0.13 mmol) was added to it and stirred for another 4 h .The reaction mixture was quenched with 6N HCl and the reaction mixture was extracted with ethyl acetate (2 x 25 mL), the organic layer was dried over anhydrous sodium sulphate, filtered and concentrated under vacuum to afford Example 74 25 (0.020 g, 40%). 'H NMR (200 MHz, CD 3 0D): 8 2.20 (s, 3H), 2.35(s, 3H), 2.89 (m, 2H), 3.33 (in, 2H), 4.20 (in, 2H), 6.51 (s, 1H), 7.20(m, 2H), 8.66 (s, 2H); m/e = 366 (M+1). 136 WO 2006/088949 PCT/US2006/005312 0 N ~ NHOH N N N H Example 75. N-hydroxy-2-(8-methyl-1,3,4,4a,5,9b-hexahydro-2H-pyrido[4,3-b]indol 2-yl)pyrimidine-5-carboxamide 5 Methyl 2-(8-methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5 carboxylate was obtained from Example 7 and 8-methyl-2,3,4,5-tetrahydro-1H-pyrido[4,3 b]indole using the method described in Example 12. To a stirred solution of methyl 2-(8 methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5-carboxylate (0.250 g, 0.776 mmol) in dry DCM, TFA (2.5 ml, 129.81 mmol) was added and stirred for 30 min. 10 Then triethylsilane (2.5 ml, 62.6 mmol) was added at room temperature and stirred for 2h. The progress of the reaction was monitored by TLC and after completion, the reaction mixture was diluted with DCM and washed with saturated solution of sodium bicarbonate and the organic layer was washed with brine, dried over anhydrous Na 2

SO

4 , filtered and concentrated under reduced pressure. The residue was purified by silica gel column 15 chromatography to furnish methyl 2-(8-methyl-1,3,4,4a,5,9b-hexahydro-2H-pyrido[4,3 b]indol-2-yl)pyrimidine-5-carboxylate (69 mg, 27.5%). 'H NMR (200 MHz, CDC1 3 ): 5 1.69 (in, 2H), 2.35 (s, 3H), 2.7 (in, 2H), 3.01 (in, 4H), 3.90 (s, 3H), 6.23 (m,1H), 6.80 (2H,m), 8.91(s, 2H); m/e = 325 (M+1). To a cooled (0 'C) solution of methyl 2-(8-methyl-1,3,4,4a,5,9b-hexahydro-2H 20 pyrido[4,3-b]indol-2-yl)pyrimidine-5-carboxylate (0.050 g, 0.15 mmol) in MeOH: DCM (6 mL, 2:1) was added aqueous 50 % NH 2 OH solution (1 mL) and NaOH (0.04 g, 1.0 mmol) dissolved in water (0.25mL). The reaction mixture was stirred for 3 h at room temperature and then concentrated under reduced pressure to give a crude residue. The residue was neutralized with 2N HCl, the precipitated solid was filtered and dried to give pure Example 25 75 (0.015 g, 30%). 'H NMR (200 MHz, CD 3 0D): 8 1.69 (m, 2H), 2.35 (s, 3H), 2.7 (in, 2H), 3.01 (in, 4H), 6.23 (m, 1H), 6.80 (2H, in), 8.65 (s, 2H); m/e = 326 (M+1). 137 WO 2006/088949 PCT/US2006/005312 0

H

2 N N NHOH

-

1 NN N H Example 76. N-hydroxy-2-(8-amino-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5- carboxamide To a solution of 4-nitrophenylhydrazine hydrochloride (3.0g, 15.8 mmol) in acetic 5 acid (30 mL) were added 4-piperidone.HCl (1.1 lg,1 1.3 mmol) and sodium acetate(1.29g, 15.7mmol). The reaction mixture temperature was raised to 80 0 C and continued stirring for lhr. The progress of the reaction was monitored by TLC and upon completion of the reaction the mixture was cooled to rt and 3 mL of concentrated sulfuric acid was aded. The reaction mixture temperature was raised to 90 0 C and continued stirring for 3hr. The acetic 10 acid was removed under reduced pressure and the pH of the reaction mixture was adjusted to 8.0 with solid K 2 C0 3 .The mixture was partitioned between ethyl acetate (100 ml) and water (35 mL). The organic layer was separated, dried over Na 2

SO

4 filtered and the solvent was removed under reduced pressure to give a crude residue, which was purified by ether washings to provide compound nitro tetrahydrocarboline (1.2g); m/e = 217 (M+1); HPLC: 15 94.9% (Rt = 11.62). To a solution of the nitro tetrahydrocarboline (1.1g, 5.0 mmol) in DMF (25mL) at rt was added Example 7 (1.642g, 7.6 mmol) and K 2 C0 3 (1.4g, 10.02 mmol). The reaction mixture temperature was raised to 100 0 C and continued stirring for 12hr. The progress of the reaction was monitored by tlc analysis and upon completion of the reaction the DMF 20 was removed under reduced pressure. The reaction was partitioned between ethyl acetate (100 mL) and water (80 mL) and the organic layer was separated, dried over sodium sulfate, filtered and the solvent was removed under reduced pressure to give a crude residue which was purified by ether washings to provide methyl-2-(8-nitro-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl)pyrimidine-5- carboxylate (0.6g,33.33%). 25 To a solution of methyl-2-(8-nitro-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxylate (0.5g, 1.41 mmol) in methanol (50 mL) was added Raney Nickel (0.3g). The reaction mixture was evacuated and stirred at rt under hydrogen 138 WO 2006/088949 PCT/US2006/005312 atmosphere over night. The progress of the reaction was monitored by TLC and upon completion of the reaction, the mixture was filtered through celite under nitrogen atmosphere and washed with methanol (2x30 mL). The methanol was removed under reduced pressure to give crude residue which was purified by column chromatography to 5 give the methyl-2-(8-amino-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5 carboxylate (0.2g, 43.9%); (Rt = 12.46). To a 0 0 C solution of methyl-2-(8-amino-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol 2-yl)pyrimidine-5-carboxylate (0,09g, 0.27 mmol) in methanol: DCM (5:2 mL) was added 50% aquesou NH20H solution (2 mL) and to the mixture was added a solution of NaOH 10 (0.08g) in water (0.5 mL). The reaction mixture was stirred at room temperature for lhr and the progress of the reaction was monitored by TLC and upon completion of the reaction the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using 1N HCl and the solid produced was filtered and washed with water followed by diethyl ether and dried under vacuum to obtain Example 76 (0.05g, 55.5%); m/e = 325 15 (M+1). O0 HN N NHOH _ NN N H Example 77. N-hydroxy-2-(8-(N-acetamido)-1,3,4,5-tetrahydro-2H-pyrido[4,3-blindol 2-yl)pyrimidine-5-carboxamide 20 To a solution of methyl-2-(8-amino-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxylate (See Example 76) (0.12g, 0.37 mmoles) in DCM (20mL) was added 4-dimethylaminopyridine (0.133g, 0.92 mmol) and acetic anhydride (0.056g, 0.55 mmol) and the mixture stirred for 1Ohr. The progress of the reaction was monitored by TLC and upon completion of the reaction the mixture was partitioned between DCM (50 mL) 25 and water (30 mL) and the organic layer was separated, dried over Na 2

SO

4 filtered and the solvent was removed under reduced pressure to give crude residue which was purified by column chromatography using basified silica gel to provide methyl-2-(8-(N-acetamido) 139 WO 2006/088949 PCT/US2006/005312 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5-carboxylate (0.040g, 29.4%) (Rt = 13.43). To a 0 'C solution of methyl-2-(8-(N-acetamido)-1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl)pyrimidine-5-carboxylate (0,04g, 0.1 1mmol) in methanol: DCM (5:2 mL) was 5 added 50% aqueous NH20H solution (1.0 mL) and to the mixture was added solution of NaOH (0.03g) in water (0.3 mL). The reaction mixture was stirred at room temperature for lhr and the progress of the reaction was monitored by TLC and upon completion of the reaction the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using IN HCI and the solid produced was filtered and washed with water 10 followed by diethyl ether and dried under vacuum to obtain Example 77 (0.014g, 27%). MS: 366 (M+1). s-o 0 HN N NHOH N N N H Example 78. N-hydroxy-2-(8-(N-methylsulfonamido)-1,3,4,5-tetrahydro-2H 15 pyrido[4,3-b]indol-2-yl)pyrimidine-5-carboxamide To a solution of methyl-2-(8-amino-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxylate (0.1g, 0.31 mmol) in DCM (20 mL) was added 4 dimethylaminopyridine (0.094g, 0.77 mmol) and methanesulfonyl chloride (0.053g, 0.46 mmol) under nitrogen atmosphere and the mixture stirred for 10 hr. The progress of the 20 reaction was monitored by TLC and upon completion of the reaction the mixture was partitioned between DCM (50 mL) and water (30 mL). The organic layer was separated, dried over Na 2

SO

4 filtered and the solvent was removed under reduced pressure to give crude residue which was purified by column chromatography using basified silica gel to provide methyl-2-(8-(N-methylsulfonamido)-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 25 yl)pyrimidine-5-carboxylate (0.08g, 64.5%) (Rt = 13.95). To a 0 C solution of the methyl-2-(8-(N-methylsulfonamido)-1,3,4,5-tetrahydro 140 WO 2006/088949 PCT/US2006/005312 2H-pyrido[4,3 -b]indol-2-yl)pyrimidine-5-carboxylate (0,07g, 0.17 mmol) in methanol: DCM (5:2mL) was added 50% aqueous ammonium hydroxide solution (1.5 mL) and to the mixture was added a solution of NaOH (0.05g) in water (0.3mL). The reaction mixture was stirred at room temperature for 1 hr and the progress of the reaction was monitored by TLC 5 and upon completion of the reaction the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using 1N HCl and the solid produced was filtered and washed with water followed by diethyl ether and was dried under vacuum to obtain Example 78 (0.03g, 42%); m/e = 403 (M+1). 10 0 N ~ NHOH N N N H Example 79. N-hydroxy-2-{8-[3-(methylsulfinyl)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide To a 0 0 C solution of methyl-2-{8-[3-(methylthio)phenyl]-1,3,4,5-tetrahydro-2H 15 pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (See Example 49) (0.1g, 0.23 mmol) in DCM (20 mL) was added 3-chloro-peroxybenzoic acid (0.04g, 0.23 mmol) and stirred for lhr under nitrogen atmosphere. The progress of the reaction was monitored by TLC and upon completion, the reaction the mixture was partitioned between DCM (50 mL) and saturated sodium bicarbonate solution (25 mL) and the organic layer was separated, washed 20 with water (2 x 25 mL), dried over sodium sulphate filtered and the solvent was removed under reduced pressure. The residue was purified by column chromatography using silica gel to provide compound methyl-2-{8-[3-(methylsulfinyl)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.08g, 77.6%). To a 0 'C solution of the methyl-2-{8-[3-(methylsulfinyl)phenyl]-1,3,4,5-tetrahydro 25 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.080g, 0.18 mmol) in methanol: DCM (5:2 mL) was added 50% aqueous hydroxylamine solution (1.6 mL) and to the mixture was added solution of NaOH (0.065g) in water (0.3 mL). The reaction mixture was 141 WO 2006/088949 PCT/US2006/005312 stirred at room temperature for lhr and the progress of the reaction was monitored by TLC. Upon completion, the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using 1N HCl and the solid produced was filtered and washed with water followed by diethyl ether and the solid was dried under vacuum to obtain Example 79 5 (0.048g, 60%). 'HNMR (DMSO-D 6 , 200 MHz) 8: 11.06 (11H, s), 8.72 (2H, s), 7.81-7.40 (Ar, 7H, in), 5.03 (2H, s), 4.25 (2H, in), 2.89 (2H, in), 2.82 (3H, s); m/e = 448 (M+1). NC 0 N - OCH 3 ___N N N H Example 80. Methyl-2-{8-[3-cyanophenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-blindol 10 2-yl}pyrimidine-5-carboxylate To a stirred solution of Example 33 (ig, 2.59 mmol) and 3-cyanophenyl boronic acid (0.761g, 5.18 mmol) in THF/water (1:1, 30 mL), tetrakis(triphenyl phosphine)palladium(0) (0.3g, 0.25 mmol), potassium carbonate (2.145g, 15.54 mmol) were added at room temperature and the resulting mixture was heated to reflux for 5-6h. The 15 progress of the reaction was monitored by TLC and after completion; the reaction was cooled to room temperature. The mixture was diluted with ethyl acetate (150 mL) and the two layers were separated. The organic layer was washed with water, dried over anhydrous sodium sulfate and concentrated in vacuum. The residue was purified by column chromatography using silica gel to give Example 80 (400 mg, 37.7%). 'HNMR (200 MHz, 20 DMSO-d 6 ) 6: 3.0 (t, 2H, CH 2 ), 3.9 (s, 3H, O.CH 3 ), 4.35 (t, 2H, CH 2 ), 5.16 (s, 2H, CH 2 ), 7.3 8.1 (in, 7H, Ar-H), 8.95 (s, 2H, pyrimidine-H); m/e = 410 (M+1).

H

2 N N OCH3 NN N H 142 WO 2006/088949 PCT/US2006/005312 Example 81. Methyl-2-{8-[3-(aminomethyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl}pyrimidine-5-carboxylate To a stirred solution of Example 80 (320 mg, 0.78 mmol) in ethanol (50 mL), Raney Ni (48 mg) was added carefully. Then the reaction mass was maintained under hydrogen 5 atmosphere overnight at room temperature. The progress of the reaction was monitored by TLC, and after completion, the reaction mass was filtered over a celite bed and the filtrate was concentrated to give Example 81 (300 mg, 92.87%). 1 HNMR (200 MHz, DMSO-D 6 ) : 2.98 (t, 2H, CH 2 ), 3.92 (s, 3H, OCH 3 ), 4.1 (s, 2H, CH 2 ), 4.35 (t, 2H, CH 2 ), 5.17 (s, 2H,

CH

2 ), 7.3-8.0 (in, 7H, Ar-H), 8.85 (s, 2H, pyrimidine-H); 11.1 (bs, 1H, NH); m/e = 415 10 (M+1). 0 0 O>NH O N NHOH N' N N H Example 82. N-hydroxy-2-{8-[3-(N-acetamidomethyl)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide 15 To a stirred solution of Example 81 (100 mg, 0.24 mmol) in dry DCM (10 mL), 4 dimethylaminopyridine (147 mg, 1.21 mmol) was added, then acetic anhydride (0.023 mL, 0.24 mmol) was added at 00 C and stirred for 5 min. The reaction mass was maintained at room temperature and stirred for lh. The progress of the reaction was monitored by TLC, and after completion, the reaction mass was extracted with DCM and water. The DCM layer 20 was dried over sodium sulfate and concentrated. The crude was purified by column chromatography to give methyl-2-{ 8-[3-(N-acetamidomethyl)phenyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (50 mg, 45.45%) with HPLC purity of 98.32%; m/e = 456 (M+1). To a stirred solution of methyl-2-{8-[3-(N-acetamidomethyl)phenyl]-1,3,4,5 25 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (50 mg, 0.10 mmol) in methanol (5 mL) and DCM (2 mL), 50% aqueous hydroxylamine hydrochloride (1 mL, 7.19 mmol) and sodium hydroxide solution (40 rag 1.0 mmol) in 0.25 mL of water were 143 WO 2006/088949 PCT/US2006/005312 added at 0 0 C and the mixture was stirred at 0 0 C for 10 min. The reaction was then allowed to come to room temperature and maintained at room temperature for lh. The progress of the reaction was monitored by TLC and after completion the solvents were removed under vacuum. The mixture was diluted with water (10 mL) and neutralized with 2N hydrochloric 5 acid (pH 6.5-7.0). The white solid that separated out was filtered and dried to give Example 82 (20 mg, 40%) of 97.64% pure by HPLC. 'IHNMR (200 MHz, DMSO-d 6 ) 5: 1.9 (s, 3H,

CH

3 ), 2.9 (t, 2H, CH 2 ), 4.2 (t, 2H, CH 2 ), 4.3 (d, 2H, CH 2 ), 5.0 (s, 2H, CH 2 ), 7.1-7.8 (in, 7H, Ar-H), 8.4 (bs, 1H, NH), 8.79 (s, 2H, Pyrimidine-H); m/e = 457 (M+1). S-NH O-I S-NH N NHOH N N N 10 H Example 83. N-hydroxy-2-{8-[3-(N-methylsulfonamidomethyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide To a stirred solution of Example 81 (150 mg, 0.36 mmol) in dry DCM (20 mL), 4 dimethylaminopyridine (221 mg, 1.81 mmol) was added, and then methanesulfonyl chloride 15 (0.029 mL, 0.36 mmol) was added at 0 0 C and stirred for 10 min. The reaction mass was maintained at room temperature and stirred for 1 hr. The progress of the reaction was monitored by TLC, and after completion, the reaction mass was extracted with DCM and water. The DCM layer dried over sodium sulfate and concentrated. The crude was purified by column chromatography to give methyl-2-{ 8-[3-(N-methylsulfonamidomethyl)phenyl] 20 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (75 mg, 30.6%) with HPLC purity (91.85%); m/e = 492 (M+1). To a stirred solution of methyl-2-{8-[3-(N-methylsulfonamidomethyl)phenyl] 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (70 mg, 0.14 mmol) in methanol (6 mL) and DCM (3 mL), 50% aqueous hydroxylamine hydrochloride 25 (2 mL, 14.38 mmol) and sodium hydroxide solution (80 mg 2.0 mmol) in 0.5 mL of water were added at 0 0 C and the mixture was stirred at 0 0 C for 10 min. The reaction was then allowed to come to room temperature and maintained at room temperature for 1h. TLC 144 WO 2006/088949 PCT/US2006/005312 monitored the progress of the reaction and after completion the solvents were removed under vacuum. The mixture diluted with water (10 mL) and neutralized with 2N hydrochloric acid (pH 6.5-7.0). The white solid that separated out was filtered and dried to give Example 83 (0.05g, 71.4%) of 95.05% purity by HPLC.- 1 HNMR (200 MHz, DMSO 5 d 6 ) 5: 2.8 (s, 3H, CH 3 ), 2.9 (t, 2H, CH 2 ), 4.2 (s, 2H, CH 2 ), 4.25 (t, 2H, CH 2 ), 5.0 (s, 2H,

CH

2 ), 7.2-7.8 (in, 7H, Ar-H), 8.78 (s, 2H, Pyrimidine-H), 10.9 (bs, 1H, NH); m/e = 493 (M+1). N'N H N NHOH N H 10 Example 84.N-hydroxy-2-{8-[3-(1H-tetrazol-5-yl)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide - To a solution of Example 80 (150mg, 0.366mmoles) in 2-methoxyethanol (20 mL) were added NaN 3 (24 mg, 0.36 mmol) and LiCl (24g, 0.52 mmol) under nitrogen atmosphere. The reaction mixture was thoroughly degassed and the temperature was raised 15 to 120 0 C and continued stirring for 4 hr. The progress of the reaction was monitored by TLC and upon completion, the reaction was quenched with cold water (5ml) and the pH was adjusted to 2 using 4N HCl. The precipitate obtained was filtered and washed with chilled acetone and the crude residue was purified by column chromatography using silica gel to provide methyl-2-{8-[3-(1H-tetrazol-5-yl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 20 b]indol-2-yl}pyrimidine-5-carboxylate (75mg, yield 45.4%); m/e = 452 (M+1). To a 0 'C solution of methyl-2-{8-[3-(1H-tetrazol-5-yl)phenyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.060g, 0.13 mmol) in methanol: DCM (5:2 mL) was added 50% aqueous hydroxylamine solution (1.2 mL) and to the mixture was added a solution of NaOH (0.050g) in water (0.3mL). The reaction mixture 25 was stirred at room temperature for 1 hr and the progress of the reaction was monitored by TLC and upon completion of the reaction the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using 1N HCl and the obtained solid was filtered 145 WO 2006/088949 PCT/US2006/005312 and washed with water followed by diethyl ether and the solid was dried under vacuum to obtain Example 84 (0.018gm). 'HNMR: DMSO-D 6 , 200 MHz) 5: 11.11 (1H, s), 8.73 (2H, s), 8.05-7.43 (Ar, 7H, m), 5.03 (2H, m), 2.99 (2H, m); m/e = 454 (M+1).

H

2 N O N ~ OCH 3 NN N 5 H Example 85. Methyl-2-{8-[3-aminophenyll-1,3,4,5-tetrahydro-2H-pyrido[4,3-blindol 2-yl}pyrimidine-5-carboxylate To a stirred solution of Example 33 (200 mg, 0.51 mmol) and 3-(tert butoxylcarbonylaminophenylboronic acid (246 mg, 1.03 mmol) in THF/water (1:1, 20 mL) 10 was added tetrakis(triphenylphosphine)palladium(0) (59 mg, 0.05 mmol) and potassium carbonate (214 mg, 1.55 mmol) and the resulting mixture was heated to reflux for 5-6h. The progress of the reaction was monitored by TLC and after completion the reaction was cooled to room temperature. The mixture was diluted with ethyl acetate (100 mL) and the two layers were separated. The organic layer was washed with water, dried over anhydrous 15 sodium sulfate and concentrated in vacuum. The residue was purified by column chromatography using silica gel to give methyl-2-{8-[3-(tert butyloxycarbonylamino)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine 5-carboxylate (160 mg, 62.0%; m/e = 500 (M+1) To a stirred solution of methyl-2- { 8-[3-(tert-butyloxycarbonylamino)phenyl] 20 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (160 mg, 0.320 mmol) in dry DCM (10 mL), TFA (0.247 mL, 3.20 mmol) was added under nitrogen atmosphere at 00 C. The reaction was maintained at room temperature for lh. The progress of the reaction was monitored by TLC and after the completion the volatiles were removed by evaporation. The crude was washed with hexane to give Example 85 (120 mg, 93.75%). 25 'HNMR (200 MHz, CD 3 0D) 8: 3.0 (t, 2H, CH 2 ), 3.9 (s, 3H, OCH 3 ), 4.42 (t, 2H, CH 2 ), 5.25 (s, 2H, CH 2 ), 7.3-7.9 (m, 7H, Ar-H), 8.9 (s, 2H, pyrimidine-H). 146 WO 2006/088949 PCT/US2006/005312

H

2 N 0 N~- NHOH N N N H Example 86. N-hydroxy-2-{8-[3-aminophenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl}pyrimidine-5-carboxamide To a stirred solution of Example 85 (120 mg, 0.30 mmol) in methanol (5 mL) and 5 DCM (3 mL), 50% aqueous hydroxylamine hydrochloride (2.5 mL, 17.97 mmol) and sodium hydroxide solution (100 mg 2.5 mmol) in 0.7 mL of water were added at 0 0 C and the mixture was stirred at 0 0 C for 10 min. The reaction was then allowed to come to room temperature and maintained at room temperature for lh. The progress of the reaction was monitored by TLC and after completion the solvents were removed under vacuum. The 10 mixture diluted with water (10 mL) and neutralized with 2N hydrochloric acid (pH 6.5-7.0). The white solid that separated out was filtered and dried to give Example 86 (0.05g) of 98.6% purity by HPLC. 'HNMR (200 MHz, CD 3 0D) 8: 3.0 (t, 2H, CH 2 ), 4.4 (t, 2H, CH 2 ), 5.16 (s, 2H, CH 2 ), 7.3-7.9 (in, 7H, Ar-H), 8.79 (s, 2H, Pyrimidine-H); m/e = 401 (M+1). HN 0 N NHOH NIN N 15 H Example 87. N-hydroxy-2-{8-[3-(N-acetamido)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-blindol-2-yl}pyrimidine-5-carboxamide To a stirred solution of Example 86 (100 mg, 0.25 mmol) in dry DCM (10 mL), 4 dimethylaminopyridine (153 mg, 1.25 mmol) was added, then acetic anhydride (0.035 mL, 20 0.37 mmol) was added at 0 0 C and the mixture was stirred for 5 min. The reaction mass was maintained at room temperature and stirred for lh. The progress of the reaction was monitored by TLC and after completion, the reaction mass was extracted with DCM. The DCM layer was dried over sodium sulfate and concentrated. The residue was purified by 147 WO 2006/088949 PCT/US2006/005312 column chromatography to give methyl-2-{8-[3-(N-acetamido)phenyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (60 mg, 54.54%). IHNMR (200 MHz, CDCl 3 ) 8: 2.21 (s, 3H, CH 3 ); 3.0 (t, 2H, CH 2 ), 3.9 (s, 3H, OCH 3 ), 4.42 (t, 2H, CH 2 ), 5.16 (s, 2H, CH 2 ), 7.3-7.8 (in, 7H, Ar-H), 8.9 (s, 2H, pyrimidine-H); m/e= 442 (M+1). 5 To a stirred solution of methyl-2-{8-[3-(N-acetamido)phenyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.1g, 0.22 mmol) in methanol (5 mL) and DCM (3 mL), 50% aqueous hydroxylamine hydrochloride (2 mL, 14.38 mmol) and sodium hydroxide solution (80 mg 2.0 mmol) in 0.5 mL of water were added at 0 0 C and the mixture was stirred at 0 0 C for 10 min. The reaction was then allowed to come to 10 room temperature and maintained at room temperature for lh. The progress of the reaction was monitored by TLC and after completion the solvents were removed under vacuum. The mixture was diluted with water (10 mL) and neutralized with 2N hydrochloric acid (pH 6.5 7.0). The white solid that separated out was filtered and dried to give Example 87 (0.05g, 50%) of 95.27% purity by HPLC. 'HNMR (200 MHz, DMSO-d 6 ) 8: 2.5 (s, 3H, CH 3 ), 2.85 15 (s, 2H, CH 2 ), 4.25 (t, 2H, CH 2 ), 5.05 (s, 2H, CH 2 ), 7.2-7.85 (m, 7H, Ar-H), 8.75 (s, 2H, Pyrimidine), 9.0 (bs, 1H, NH), 10.0 (bs, 1H, NH-OH), 11.0 (bs, 1H, OH); m/e = 443 (M+1). HN O OSN N NHOH -- I N H Example 88. N-hydroxy-2-{8-[3-(N- methylsulfonamido)phenyl]-1,3,4,5-tetrahydro 20 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide To a stirred solution of Example 86 (100 mg, 0.25 mmol) in dry DCM (10 mL), 4 dimethylaminopyridine (153 mg, 1.25 mmol) was added, then methanesulfonyl chloride (0.03 mL, 0.37 mmol) was added at 0 0 C and stirred for 10 min. The reaction mass was maintained at room temperature and stirred for lh. The progress of the reaction was 25 monitored by TLC, and after completion, the reaction mass was extracted with DCM and water. The DCM layer was dried over sodium sulfate and concentrated. The crude was purified by column chromatography to give methyl-2-{8-[3-(N 148 WO 2006/088949 PCT/US2006/005312 methylsulfonamido)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5 carboxylate (45 mg, 37.8%). 'HNMR (200 MHz, CDCl 3 ) 8: 2.98 (t, 2H, CH 2 ), 3.1 (s, 3H,

CH

3 ), 3.9 (s, 3H, OCH 3 ), 4.42 (t, 2H, CH 2 ), 5.17 (s, 2H, CH 2 ), 7.3-7.8 (in, 7H, Ar-H), 7.98 (bs, 1H, NH), 8.9 (s, 2H, pyrimidine-H); m/e = 443 (M+1) 5 To a stirred solution of methyl-2-{ 8-[3-(N- methylsulfonamido)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (45 mg, 0.09 mmol) in methanol (3 mL) and DCM (2 mL), 50% aqueous hydroxylamine hydrochloride (1 mL, 7.19 mmol) and sodium hydroxide solution (40 mg 1.0 mmol) in 0.25 mL of water were added at 0 0 C and the mixture was stirred at 0 0 C for 10 min. The reaction was then allowed 10 to come to room temperature and maintained at room temperature for lh. The progress of the reaction was monitored by TLC and, after completion, the solvents were removed under vacuum. The residue was diluted with water (10 mL) and neutralized with 2N hydrochloric acid (pH 6.5-7.0). The white product that separated out was filtered and dried to give Example 88 (20 mg, 44.45%) of 95.27% purity by HPLC. 'IHNMR (200 MHz, CD 3 0D) 5: 15 2.98 (t, 2H, CH 2 ), 3.1 (s, 3H, CH 3 ), 4.2 (t, 2H, CH 2 ), 5.12 (s, 2H, CH 2 ), 7.18-7.75 (in, 7H, Ar-H), 8.78 (s, 2H, Pyrimidine), 11.0 (bs, 1H, NH). OHC 0 N ~ OCH 3 N N N H Example 89. Methyl-2-{8-[3-(formyl)phenyll-1,3,4,5-tetrahydro-2H-pyrido[4,3 20 blindol-2-yl}pyrimidine-5-carboxylate To a solution of Example 33 (0.650g, 1.67 mmol) in THF:H 2 0 (4:1) was added

K

2 C0 3 (0.579g, 4.19 mmol) and 3-fonnylphenylboronic acid (0.501g, 3.36 mmol). The reaction mixture was thoroughly degassed and freshly prepared tetrakis(triphenylphosphine)palladium(0) (0.387g, 0.33 mmol) was added under nitrogen 25 atmosphere at room temperature. The reaction mixture temperature was raised to 80 OC and stirring continued for 12hr. The progress of the reaction was monitored by TLC and upon completion of the reaction the THF was removed under reduced pressure. The mixture was 149 WO 2006/088949 PCT/US2006/005312 partitioned between ethyl acetate (100 mL) and water (15 mL). The organic layer was separated, dried over Na 2

SO

4 filtered and the solvent was removed under reduced pressure to give crude residue, which was purified by column chromatography using silica gel to provide Example 89 (0.35g, 50.4%); 1 HNMR (CDCl 3 , 200 MHz) 8: 10.11 (s,1H), 8.90 5 (s,2H), 8.17 (s,1H), 8.00-7.92 (m,2H), 7.85-7.80 (m,2H), 7.64,7.38 (m,4H), 5.15 (s,2H), 4.39 (t, 2H, J=5.4Hz), 3.88 (s,3H), 2.98 (t, 2H, J=5.4Hz); m/e = 413 (M+1). N0 NN NHOH - NN N H Example 90. N-hydroxy-2-{8-[3-(morpholin-4-ylmethyl)phenyl]-1,3,4,5-tetrahydro 10 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide To a stirred solution of Example 89 (0.110 mg, 0.26 mmol) in dry DCM (10 mL) were added morpholine (0.349g, 4.00 mmol) and sodium triacetoxyborohydride (0.422g, 2.00 mmol) at room temperature and the mixture was stirred at the same temperature overnight. After completion, the reaction was diluted with DCM (40 mL) and washed with 15 saturated sodium bicarbonate solution and water. The organic layer was dried over anhydrous sodium sulfate and concentrated in vacuum. The residue was purified by column chromatography to give methyl-2-{ 8-[3-(morpholin-4-ylmethyl)phenyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.08g, 62%). 'HNMR (200 MHz, CDCl 3 ) 6: 2.58 (in, 4H, 2 x CH 2 ), 3.0 (t, 2H, CH 2 ), 3.62 (in, 2H, CH 2 ), 3.75 (in, 4H, 2 x 20 CH 2 ), 3.9 (s, 3H, OCH 3 ), 4.41 (t, 2H, CH 2 ), 5.18 (s, 2H, CH 2 ), 7.38-7.5 (in, 3H, Ar-H), 7.5 7.7 (in, 2H, Ar-H), 7.8 (s, 1H, Ar-H), 7.92 (s, 1H, Ar-H), 8.95 (s, 2H, pyrimidine-H); m/e = 484 (M+ 1). To a stirred solution of methyl-2-{8-[3-(morpholin-4-ylmethyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.0 8 g, 0.16 mmol) in 25 methanol (4 mL) and DCM (2 mL), 50% aqueous hydroxylamine hydrochloride (1.6 mL, 11.51 mmol) and sodium hydroxide solution (65 mg 1.62 mmol) in 0.5 mL of water were added at 00 C and the mixture was stirred at 0 0 C for 10 min. The reaction was then allowed 150 WO 2006/088949 PCT/US2006/005312 to come to room temperature and maintained at room temperature for 1h. The progress of the reaction was monitored by TLC and after completion the solvents were removed under vacuum. The mixture was diluted with water (10 mL) and neutralized with 2N hydrochloric acid (pH 6.5-7.0). The white product that separated out was filtered and dried to give 5 Example 90 (0.05g, 62.5%) of 94.5% purity by HPLC. 'HNMR (200 MHz, DMSO-d 6 ) 5: 2.4 (in, 4H, CH 2 ), 2.9 (t, 2H, CH 2 ), 3.46 (s, 2H, CH 2 ), 3.58 (in, 4H, 2 x CH 2 ), 4.25 (t, 2H,

CH

2 ), 5.0 (s, 2H, CH 2 ), 7.2 (d, 1H, Ar-H), 7.32-7.65 (in, 5H, Ar-H), 7.8 (s, 1H, Ar-H), 8.7 (s, 2H, Pyrimidine-H), 11.0 (bs, 1H, NH); m/e = 485 (M+1). Examples 91-97 were synthesized in the same manner as Example 90 by substituting 10 the appropriate amine for morpholine in the reaction. N0 N NHOH - NN N H Example 91. N-hydroxy-2-{8-[3-(piperidin-1-ylmethyl)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide 15 Example 91 was obtained by substituting piperidine for morpholine in the reaction with Example 89. A white solid (0.05g, 50%) of 95.27% purity by HPLC. 'HNMR (200 MHz, CD 3 0D) 6: 1.5 (in, 2H, CH 2 ) 1.7 (m, 4H, 2 x CH 2 ), 2.63 (in, 4H, 2 x CH 2 ), 3.0 (t, 2H,

CH

2 ), 3.79 (s, 2H, CH 2 ), 4.4 (t, 2H, CH 2 ), 5.18 (s, 2H, CH 2 ), 7.3 (d, 1H, Ar-H), 7.4-7.8 (in, 6H, Ar-H), 8.79 (s, 2H, Pyrimidine-H); m/e = 483 (M+1). 20 -NJN 0 NHOH 'NN N H Example 92. N-hydroxy-2-{8-[3-(NN-dimethylaminomethyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide 151 WO 2006/088949 PCT/US2006/005312 Example 92 was obtained by substituting dimethylamine for morpholine in the reaction with Example 89. A white solid (50mg, 50%) of 93.98% purity by HPLC. 'HNMR (200 MHz, CD 3 0D) 5: 2.8 (s, 6H, 2 x CH 3 ), 2.99 (t, 2H, CH 2 ), 4.18 (s, 2H, CH 2 ),), 4.4 (t, 2H, CH 2 ), 5.18 (s, 2H, CH 2 ),), 7.3-7.75 (m, 7H, Ar-H), 8.7 (s, 2H, Pyrimidine-H); m/e 5 444 (M+1). \ / 0 N NN1 NHOH OH N H Example 93. N-hydroxy-2-{8-[3-((4-hydroxymethylpiperidin-1-yl)methyl)phenyll 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide 10 Example 93 was obtained by substituting 4-hydroxymethylpiperidine for morpholine in the reaction with Example 89. A white solid (40 mg, 40%) of 97.6% purity by HPLC. 'HNMR (200 MHz, CD 3 0D) 8: 1.3 (m, 3H, CH 2 , CH), 1.8 (m, 2H, CH 2 ), 2.3 (t, 2H, CH 2 ), 2.99 (t, 2H, CH 2 ), 3.2 (m, 2H, CH 2 ), 3.43 (d, 2H, CH 2 ), 3.8 (s, 2H, CH 2 ), 4.4 (t, 2H, CH 2 ), 5.18 (s, 2H, CH 2 ), 7.2 -7.8 (m, 7H, Ar-H), 8.8 (s, 2H, Pyrimidine-H); m/e = 513 (M+1). 15 N N NHOH - NIN N H Example 94. N-hydroxy-2-{8-[3-(thiomorpholin-4-ylmethyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-blindol-2-yl}pyrimidine-5-carboxamide Example 94 was obtained by substituting thiomorpholine for morpholine in the 20 reaction with Example 89. A white solid (70 mg, 70%) of 97.25% purity by HPLC. 'HNMR (200 MHz, CD 3 0D) 8: 2.75 (t, 4H, 2 x CH 2 ), 2.82 (t, 4H, 2 x CH 2 ), 2.9 (t, 2H,

CH

2 ), 3.7 (s, 2H, CH 2 ), 4.4 (t, 2H, CH 2 ), 516 (s, 2H, CH 2 ), 7.2-7.8 (m, 7H, Ar-H), 8.79 (s, 2H, Pyrimidine-H); m/e = 501 (M+1). 152 WO 2006/088949 PCT/US2006/005312 0 HO N NHOH N N OH ~~ N H Example 95. N-hydroxy-2-{8-[3-(NN-di(2-hydroxyethyl)aminomethyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide 5 Example 95 was obtained by substituting NN-di(2-hydroxyethyl)amine for morpholine in the reaction with Example 89. A white solid (27 mg, 64.2%) with 97.65% purity (by HPLC). 'HNMR (200 MHz, CD 3 0D) 8: 2.8 (t, 4H, 2 x CH 2 ), 2.99 (t, 2H, CH 2 ), 3.7 (t, 4H, 2 x CH 2 ), 3.85 (s, 2H, CH 2 ), 4.4 (t, 2H, CH 2 ), 5.18 (s, 2H, CH 2 ), 7.2 -7.8 (m, 7H, Ar-H), 8.8 (s, 2H, Pyrimidine-H); m/e = 503 (M+1). 10 0 N N- NHOH / - NN N H Example 96. N-hydroxy-2-{8-[3-((4-methylpiperazin-1-yl)methyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide Example 96 was obtained by substituting 4-methylpiperazine for morpholine in the 15 reaction with Example 89. A white solid (0.114g). 1 HNMR (DMSO-d 6 ) 8:10.996 (s,1H), 8.728 (s,2H), 7.759 (s,1H), 7.765 (d,2H), 7.356 (m,5H), 5.016 (s,2H), 4.252 (m,2H), 2.891 (m,2H), 3.869 (s, 2H). 0 N 0 NHOH

NI

N H 153 WO 2006/088949 PCT/US2006/005312 Example 97. N-hydroxy-2-{8-[3-(pyrrolidin-1-ylmethyl)phenyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide Example 97 was obtained by substituting pyrrolidine for morpholine in the reaction with Example 89. A white solid (0.46g,95.5%). HPLC (Rt = 12.71); m/e = 469 (M+1). 5 N0 NN

OCH

3 N N o N H Example 98. Methyl-2-{8-[3-(N-tert-butylcarbonylpiperazin-1-ylmethyl)phenyl] 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate To a stirred solution of Example 89 (1.0g, 2.42 mmol) in dry DCM (100 mL) was 10 added N-tert-butylcarbonylpiperazine (4.5 1g, 24.24 mmol) and sodium triacetoxyborohydride (2.56g, 12.13 mmol) at room temperature and the mixture was stirred at the same temperature overnight. After completion, the reaction was diluted with DCM (400 mL) and washed with saturated sodium bicarbonate solution and water. The organic layer was dried over anhydrous sodium sulfate and concentrated in vacuum. The residue 15 was purified by column chromatography to give Example 98 (1.0g, 70.82%). 1 HNMR (200 MHz, CDCl 3 ) 8: 1.5 (s, 9H, 3 x CH 3 ), 2.45 (t, 4H, 2 x CH 2 ), 3.0 (t, 2H, CH 2 ), 3.42 (t, 4H, 2 x CH 2 ), 3.6 (s, 2H, CH 2 ), 3.92 (s, 3H, OCH 3 ), 4.4 (t, 2H, CH 2 ), 5.18 (s, 2H, CH 2 ), 7.3-7.8 (m, 7H, Ar-H), 8.9 (s, 2H, Pyrimidine-H); m/e = 583 (M+1). N0 HN NHOH HN NIN' N 20 H Example 99. N-hydroxy-2-{8-[3-(piperazin-1-ylmethyl)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide To a 0 0 C solution of Example 98 (0.180g) in DCM (1OmL) was added ether/HCl 154 WO 2006/088949 PCT/US2006/005312 (1 Oml) and the mixture stirred for 30 min. The progress of the reaction was monitored by TLC and solvent was evaporated and washed with ether to give methyl-2-{8-[3-(piperazin 1-ylmethyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5 carboxylate (0.16g, 78.36%). 5 To a 0 'C solution of methyl-2-{8-[3-(piperazin-1-ylmethyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.16g) in methanol :DCM (7.5:3.5) was added 50% aqueous hydroylamine solution (3.5 mL) and to the mixture was added a solution of NaOH (125 mg) in water (.75mL). The reaction mixture was stirred at room temperature for lhr and the progress of the reaction was monitored by 10 TLC analysis and upon completion of the reaction the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using 1N hydrochloric acid and the obtained solid was filtered and washed with water followed by diethyl ether. The solid was dried under vacuum to obtain Example 99 (0.16g,99.78%); m/e = 484 (M+1). N0 NN NHOH N NNN 15 H Example 100. N-hydroxy-2-{8-[3-((4-(methylsulfonyl)piperazin-1-yl)methyl)phenyl] 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide To a stirred solution of Example 98 (1.0g, 1.72 mmol) in dry DCM (100 mL) TFA (0.979g, 8.58 mmol) was added under nitrogen atmosphere at 00 C. The reaction was 20 maintained at room temperature for 1h. The progress of the reaction was monitored by TLC and after the completion the volatiles were removed by evaporation. The crude was washed with hexane to give methyl-2- { 8- [3 -(piperazin- 1 -ylmethyl)phenyl]- 1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (800 mg, 96%). To a stirred solution of methyl-2-{8-[3-(piperazin-1-ylmethyl)phenyl]-1,3,4,5 25 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (800 mg, 1.65 mmol) in dry DCM (50 mL) was added 4-dimethylaminopyridine (1.012g, 8.29 mmol), then 155 WO 2006/088949 PCT/US2006/005312 methanesulfonyl chloride (0.155mL, 1.99 mmol) was added at 0 0 C and stirred for 10 min. The reaction mass was maintained at room temperature and stirred for lh. The progress of the reaction was monitored by TLC and after completion the reaction mass was diluted with water and extracted with DCM. The DCM layer dried over sodium sulfate and concentrated. 5 The residue was purified by column chromatography to give methyl-2-{8-[3 -((4 (methylsulfonyl)piperazin-1-yl)methyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl}pyrimidine-5-carboxylate (900 mg, 95.40%) with HPLC purity 95.40%. 'HNMR (200 MHz, CD 3 0D) 8: 2.62 (t, 4H, 2 x CH 2 ), 2.85 (s, 3H, CH 3 ) 3.0 (t, 2H, CH 2 ), 3.22 (t, 4H, 2 x

CH

2 ), 3.7 (s, 2H, CH 2 ) 3.95 (s, 3H, OCH 3 ), 4.4 (t, 2H, CH 2 ), 5.18 (s, 2H, CH 2 ), 7.2-7.8 (in, 10 7H, Ar-H), 8.9 (s, 2H, Pyrimidine-H); m/e = 561 (M+1). To a stirred solution of methyl-2-{8-[3-((4-(methylsulfonyl)piperazin-1 yl)methyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.90g, 1.60 mmol) in methanol (50 mL) and DCM (25 mL), 50% aqueous hydroxylamine hydrochloride (20 mL, 143.88 mmol) and sodium hydroxide solution (800 mg 20 mmol) in 15 5 mL of water were added at 0 "C and the mixture was stirred at 0 0 C for 10 min. The reaction was then allowed to come to room temperature and maintained at room temperature for 1 h. The progress of the reaction was monitored by TLC and after completion the solvents were removed under vacuum. The mixture diluted with water (10 mL) and neutralized with 2N hydrochloric acid (pH 6.5-7.0). A white solid separated out which and 20 was filtered and dried to give Example 100 (900 mg, 93%) with HPLC purity 96.16%. 'HNMR (200 MHz, CD 3 0D) 8: 2.62 (t, 4H, 2 x CH 2 ), 2.85 (s, 3H, CH 3 ) 3.0 (t, 2H, CH 2 ), 3.22 (t, 4H, 2 x CH 2 ), 3.7 (s, 2H, CH 2 ) 4.4 (t, 2H, CH 2 ), 5.18 (s, 2H, CH 2 ), 7.2-7.8 (in, 7H, Ar-H), 8.9 (s, 2H, Pyrimidine-H); m/e = 562 (M+1). N0 N NHOH N N I N) O=C~- | NNN 25 H Example 101. N-hydroxy-2-{8-[3-((4-acetylpiperazin-1-yl)methyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-blindol-2-yl}pyrimidine-5-carboxamide 156 WO 2006/088949 PCT/US2006/005312 To a stirred solution of methyl-2-{8-[3-(piperazin-1-ylmethyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (1.5g, 3.11 mmol) in dry DCM (50 mL) was added 4-dimethylaminopyridine (1.9g, 15.57 mmol) and then acetic anhydride (0. 35 mL, 3.72 mmol) was added at 0 0 C and stirred for 5 min. The reaction 5 mass was maintained at room temperature and stirred for 1h. The progress of the reaction was monitored by TLC, and after completion the reaction mass was diluted with water and extracted with DCM. The DCM layer dried over sodium sulfate and concentrated. The residue was purified by column chromatography to give methyl-2-{8-[3-((4 acetylpiperazin-1-yl)methyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 10 yl}pyrimidine-5-carboxylate (1.4g, 85.88%) with HPLC purity 99.25%; m/e = 525 (M*+1). To a stirred solution of methyl-2-{8-[3-((4-acetylpiperazin-1-yl)methyl)phenyl] 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (1.4g, 2.67 mmol) in methanol (50 mL) and DCM (25 mL), 50% aqueous hydroxylamine hydrochloride (28m, 201.43 mmol) and sodium hydroxide solution (1.2g 30 mmol in 12 mL of water) were 15 added at 0 0 C and the mixture was stirred at 0 0 C for 10 min. The reaction was then allowed to come to room temperature and maintained at room temperature for 1h. The progress of the reaction was monitored by TLC and after completion the solvents were removed under vacuum. The mixture diluted with water (50 mL) and neutralized with 2N hydrochloric acid (pH 6.5-7.0). A white product separated out and was filtered and dried to give Example 101 20 (1.1g, 78.57%) of 94.95% purity by HPLC. 'HNMR (200 MHz, CD 3 0D) 5: 2.1 (s, 3H,

CH

3 ), 2.2.58 (in, 4H, 2 x CH2), 2.97 (t, 2H, CH 2 ), 3.6 (t, 4H, 2 x CH 2 ), 3.62 (s, 2H, CH 2 ), 4.4 (t, 2H, CH 2 ), 5.18 (s, 2H, CH 2 ), 7.22-7.8 (in, 7H, Ar-H), 8.7 8 (s, 2H, Pyrimidine-H); m/e = 525.8 (M+1). 0 \/ 0 N

OCH

3 N 25 H 157 WO 2006/088949 PCT/US2006/005312 Example 102. Methyl-2-{8-[4-(formyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 blindol-2-yl}pyrimidine-5-carboxylate To a 0 0 C solution of Example 33 (1.5g, 3.37 mmol) in THF:H 2 0 (4:1) were added Cs 2

CO

3 (10.07g, 30.99mmol) and 4-fonnylphenylboronic acid (1.158g, 7.75 mmol). The 5 reaction mixture was thoroughly degassed and freshly prepared tetrakis(triphenylphosphine)palladium(0) (1.1 19g, 0.96 mmol) was added under nitrogen atmosphere at room temperature. The reaction mixture temperature was raised to 80 0 C and continued stirring for 12hr. The progress of the reaction was monitored by TLC and upon completion of the reaction the THF was removed under reduced pressure. The mixture was 10 partitioned between ethyl acetate (100 mL) and water (15 mL). The organic layer was separated, dried over Na 2

SO

4 filtered and the solvent was removed under reduced pressure to give crude residue, which was purified by column chromatography using silica gel to provide Example 102 (1.0, 62.6%). 'HNMR (CDCl 3 , 200 MHz) 8: 10.11 (s, 1H), 8.90 (s, 2H), 8.17 (s, 1H), 8.00-7.92 (m, 2H), 7.85-7.80 (m, 2H), 7.64-7.38 (m,4H), 5.15 (s,2H), 15 4.39 (t, 2H, J=5.4Hz), 3.88(s, 3H), 2.98 (t, 2H, J=5.4Hz); m/e = 413(M+1). 0 N'N NHOH N N N H Example 103. N-hydroxy-2-{8-[4-(pyrrolidin-1-ylmethyl)phenyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-blindol-2-yl}pyrimidine-5-carboxamide 20 To a 0 0 C solution of Example 102 (1.0g, 2.42 mmol) in DCM (50 mL) was added Na(OAc) 3 BH (1.028g, 4.8 mmol) and stirred for 10 min. To the reaction mixture was added pyrrolidine (0.861g, 12.13 mmol) under nitrogen atmosphere and the reaction mixture was continued stirring at room temperature for 4hr. The progress of the reaction was monitored by TLC and upon completion of the reaction the mixture was partitioned between DCM 25 (1 OOmL) and water (1 5mL) and the organic layer was separated, washed with water (2 x 10 158 WO 2006/088949 PCT/US2006/005312 mL), dried over sodium sulfate filtered and the solvent was removed under reduced pressure to give crude residue, which was purified by column chromatography using silica gel to provide methyl-2-{8-[4-(pyrrolidin-1-ylmethyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl}pyrimidine-5-carboxylate (2g, 95%). 5 To a 0 0 C solution of methyl-2-{8-[4-(pyrrolidin-1-ylmethyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (1.23g) in MeOH: DCM (30:12mL) was added 50% aqueous hydroylamine solution (24 mL) and to the mixture was added solution of NaOH (960mg) in water (7mL). The reaction mixture was stirred at room temperature for lhr and the progress of the reaction was monitored by TLC and upon 10 completion of the reaction the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using 1N HCl and the obtained solid was filtered and washed with water followed by diethyl ether and dried under vacuum to give Example 103 (1.24g). 1 HNMR (CD 3 0D, 200 MHz) 8: 8.74 (s, 2H), 7.70 (d, 4H), 7.39 (in, 5H), 5.08 (s, 2H), 4.36 (in, 2H), 3.87 (s, 2H), 2.95 (in, 2H), 2.81 (in, 4H), 1.92 (in, 4H); m/e = 468 (M+1). 15 Examples 104 to 106 were prepared in the same manner as Example 103 by substituting the appropriate amine for pyrrolidine in the reaction with Example 33. 0 N 0 N NHOH N N N H Example 104. N-hydroxy-2-{8-[4-(morpholin-4-ylmethyl)phenyl]-1,3,4,5-tetrahydro 20 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide Example 104 was obtained by substituting morpholine for pyrrolidine in the reaction with Example 33. A solid (0.023g, 15.26%); 'IHNMR (CD 3 0D, 200 MHz) 8: 8.74 (2H, s), 7.67-7.37 (8H, in), 5.09 (2H, s), 4.37 (2H, in), 2.96 (2H, in), 3.76 (4H, in), 2.56 (4H, in), 3.59 (2H, s); m/e = 485 (M+1). 25 159 WO 2006/088949 PCT/US2006/005312 S N 0 N NHOH N N N H Example 105. N-hydroxy-2-{8-[4-(thiomorpholin-4-ylmethyl)phenyl-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide Example 105 was obtained by substituting thiornorpholine for pyrrolidine in the 5 reaction with Example 33. A solid (0.035g, 49.91%); 'HNMR (CD 3 0D, 200 MHz) 8: 8.74 (s, 2H), 7.68 (d, 4H), 7.37 (d, 4H), 5.09 (s,2H), 4.34 (s, 2H), 3.68 (s, 2H), 2.84 (m, 2H), 2.84 (m, 4H), 2.72 (m,4H); m/e = 500 (M+1). / N 0 N' NHOH N H 10 Example 106. N-hydroxy-2-{8-[4-((4-methylpiperazin-1-yl)methyl)phenyl]-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide Example 106 was obtained by substituting N-methylpiperazine for pyrrolidine in the reaction with Example 33. A solid (0.005g); HPLC (Rt-1 1.77 min). 'HNMR (DMSO-d 6 ) (200 MHz) 8: 10.99 (1H, s), 8.72 (2H, s), 7.75 (1H, s), 7.67 (2H, d), 7.35 (5H, m), 5.01 (s, 15 2H), 4.25 (2H, m), 2.89 (2H, m), 3.38 (2H, s), 2.49 (8H, m), 2.24 (3H, m); m/e = 498 (M+1). 160 WO 2006/088949 PCT/US2006/005312 OH \ / 0 N NHOH NIN N H Example 107. N-hydroxy-2-{8-[4-(hydroxymethyl)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide To a solution of Example 102 (0.150g, 0.36 mmol) in DCM was added NaBH 4 5 (0.069g, 1.81 mmol) under nitrogen atmosphere at 0 0 C. The reaction mixture temperature was raised to room temperature and continued stirring for 3hr. The progress of the reaction was monitored by TLC and upon completion, the mixture was partitioned between DCM (25 mL) and water (15 mL) and the organic layer was separated, dried over Na 2

SO

4 filtered and the solvent was removed under reduced pressure to give crude residue, which was 10 purified by column chromatography using silica gel to provide methyl-2-{8-[4 (hydroxymethyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5 carboxylate (0.050g, 33.2%). To a 0 0 C solution of methyl-2-{ 8-[4-(hydroxymethyl)phenyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.050g) in methanol: DCM (5:2 mL) 15 was added 50% aqueous hydroxylamine solution (1 mL) and to the mixture was added solution of NaOH (0.04g) in water (0.2 mL). The reaction mixture was stirred at room temperature for lhr and the progress of the reaction was monitored by TLC and upon completion the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using 1N HCl and the obtained solid was filtered and washed with water 20 followed by diethyl ether and dried under vacuum to give Example 107 (0.022g, 44%). HPLC (Rt-12.84); 'IHNMR (DMSO-D 6 , 200 MHz) 8: 10.98 (2H, s), 8.80 (2H, s), 7.76-7.35 (Ar, 7H, in), 5.02 (2H, s), 4.53 (2H, s), 4.25 (2H, in), 2.89 (2H, in); m/e = 416 (M+1). 161 WO 2006/088949 PCT/US2006/005312 N 0 NN NHOH ZN AN N H Example 108. N-hydroxy-2-{8-[5-((4-methylpiperazin-1-yl)methyl)-2-furyl-1,3,4,5 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide To a solution of Example 33 (0.25g, 0.64 mmol) in THF:H 2 0 (4:1) were added 5 Cs 2

CO

3 (1.689g, 5.16 mmol) and 5-formyl-2-furanboronic acid (0.180g, 1.29mmol). The reaction mixture was thoroughly degassed and freshly prepared tetrakis(triphenylphosphine)palladium(0) (0.1 86g, 0.16 mmol) was added under nitrogen atmosphere at room temperature. The reaction mixture temperature was raised to 80 0 C and continued stirring for 12hr. The progress of the reaction was monitored by TLC analysis and 10 upon completion of the reaction the THF was removed under reduced pressure. The mixture was partitioned between ethyl acetate (100 mL) and water (15 mL). The organic layer was separated, dried over Na 2

SO

4 filtered and the solvent was removed under reduced pressure to give crude residue, which was purified by column chromatography using silica gel to give methyl-2-{8-[5-(formyl)-2-furyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 15 yl}pyrimidine-5-carboxylate (0.12g, 46.2%) To a 0 0 C solution of methyl-2-{8-[5-(fonnyl)-2-furyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.12g, 0.29 mmol) in DCM (20mL) was added Na(OAc) 3 BH (0.126g, 0.59 mmol) and stirred for 10min. To the reaction mixture was added N-methylpiperzine (0. 149g, 1.49 mmol) under nitrogen atmosphere and the reaction 20 mixture was continued stirring at room temperature for 4 hr. The progress of the reaction was monitored by TLC and upon completion of the reaction the mixture was partitioned between DCM (50 mL) and water (25 mL) and the organic layer was separated, washed with water (2 x 25 mL), dried over sodium sulfate filtered and the solvent was removed under reduced pressure to give crude residue, which was purified by column 25 chromatography using silica gel to provide methyl-2-{8-[5-((4-methylpiperazin-1 yl)methyl)-2-furyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5 carboxylate (0.1g, 69.4%). 162 WO 2006/088949 PCT/US2006/005312 To a 0 'C solution of methyl-2-{8-[5-((4-methylpiperazin-1-yl)methyl)-2-furyl] 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.1 g) in methanol :DCM (5:2 mL) was added 50% aqueous hydroxylamine solution (2 mL) and to the mixture was added solution of NaOH (0.8g) in water (0.5mL). The reaction mixture was 5 stirred at room temperature for 1hr and the progress of the reaction was monitored by TLC and upon completion of the reaction the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using IN HCl and the obtained solid was filtered and washed with water followed by diethyl ether and dried under vacuum to give Example 108 (0.08, 64.87%). HPLC: (Rt = 11.58); m/e = 488 (M+1). 10 N *" 0 N N NHOH N N N H Example 109. N-hydroxy-2-{8-[5-(morpholin-4-yl)methyl-2-furyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide Example 109 was prepared in the same manner as Example 108 by substituting 15 morpholine for N-methylpiperazine in the reaction sequence. A solid (0.075g, 48.89%); HPLC: (Rt = 12.01 min.); m/e = 475 (M+1). N O N N NHOH - N N N H Example 110. N-hydroxy-2-{8-[5-((4-methylpiperazin-1-yl)methyl)-thien-2-yl]-1,3,4,5 20 tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide To a solution of Example 33 (0.5g, 1.29 mmol) in THF:H 2 0 (4:1) were added Cs 2

CO

3 (3.354g, 10.32 mmol) and 5-fornyl-2-thiopheneboronic acid(0.503g, 3.22 mmol). The reaction mixture was thoroughly degassed and freshly prepared 163 WO 2006/088949 PCT/US2006/005312 tetrakis(triphenylphosphine)palladium(0) (0.446g, 0.38 mmol) was added under nitrogen atmosphere at room temperature. The reaction mixture temperature was raised to 80 0 C and continued stirring for 12hr. The progress of the reaction was monitored by TLC and upon completion of the reaction the THF was removed under reduced pressure. The mixture was 5 partitioned between ethyl acetate (100 mL) and water (15 mL) and the organic layer was separated, dried over Na 2

SO

4 filtered and the solvent was removed under reduced pressure to give crude residue, which was purified by column chromatography using silica gel to provide methyl-2-{8-[5-(formyl)-thien-2-yl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl}pyrimidine-5-carboxylate (0.1 g). 10 To a 0 0 C solution of methyl-2-{8-[5-(formyl)-thien-2-yl]-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.09g, 0.21 mmol) in DCM(50 mL) was added Na(OAc) 3 BH (0.114g, 0.53 mmol) and stirred for 10 min. To the reaction mixture was added N-methylpiperazine (0.107g, 1.07 mmol) under nitrogen atmosphere and the reaction mixture was continued stirring at room temperature for 4hr. The progress of the 15 reaction was monitored by TLC and upon completion of the reaction the mixture was partitioned between DCM (100 mL) and water (15 mL) and the organic layer was separated, washed with water (2 x 10 mL), dried over sodium sulphate filtered and the solvent was removed under reduced pressure to give crude residue, which was purified by column chromatography using silica gel to provide methyl-2-{8-[5-((4-methylpiperazin-1 20 yl)methyl)-thien-2-yl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5 carboxylate (0.08g). To a 0 'C solution of the methyl-2-{8-[5-((4-methylpiperazin-1-yl)methyl)-thien-2 yl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxylate (0.08g, 0.165 mmol) in methanol: DCM (5:2 mL) was added 50% aqueous hydroxylamine solution (1.5 25 mL) and to the mixture was added solution of NaOH (0.060g) in water (0.3mL). The reaction mixture was stirred at room temperature for 1 hr and the progress of the reaction was monitored by TLC and upon completion of the reaction the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using IN HCl and the obtained solid was filtered and washed with water followed by diethyl ether and the solid was dried 30 under vacuum to give Example 110 (0.040g). 'IHNMR (DMSO-D 6 , 200 MHz) 5: 11.01 (1H, 164 WO 2006/088949 PCT/US2006/005312 s), 8.70 (2H, s), 7.70 (1H, s), 7.29 (2H, m), 7.21(1H, s), 6.89 (1H, s), 4.95 (s, 2H), 4.20 (2H, m), 3.63 (2H, s), 2.85 (2H, mn), 2.49-2.32 (8H, m), 2.14 (3H, s); m/e = 504 (M+1). 0 F N NHOH

NIN'

- NN N H 5 Example 111. N-hydroxy-2-(8-fluoro-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxamide Example 111 was obtained by substituting 4-fluorophenylhydrazine for 4 bromophenylhydrazine in the reaction sequence. A solid; 'H NMR (DMSO-d 6 , 300 MHz) 8; 2.82 (m, 2H), 4.23 (m, 2H), 4.90 (s, 2H), 6.85 (t, J= 9 Hz, 1H), 7.27 (m, 2H), 8.70 (s, 10 2H), 11.02 (s, 1H), 11.08 (s, 1H); m/e = 316 (M+1). 0 N NHOH Br- N N NO H Example 112. N-hydroxy-2-(7-bromo-1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl)pyrimidine-5-carboxamide 15 Example 112 was obtained by substituting 3-bromophenylhydrazine for 4 bromophenylhydrazine in the reaction sequence. A white solid (12 mg); 'HNMR (200 MHz, DMSO-d 6 ) 8: 8.73 (2H, s), 7.46 (1H, s), 7.39 (2H, d, J= 8.4Hz), 7.15 (2H, d, J= 8.4Hz), 5.03 (2H, s), 4.34 (2H, t, J= 5.6Hz), 2.93 (2H, t, J= 5.6Hz); m/e = 387.8 (M+1). 165 WO 2006/088949 PCT/US2006/005312 0 N NHOH 'O N N aN H Example 113. N-hydroxy-2-(9-methoxy-1,4,5,6-tetrahydroazepino[4,5-blindol-3(2H) 1-yl)pyrimidine-5-carboxamide To tert-butyl 4-oxoazepane-1-carboxylate (1g, 4.68 mmol) and 4 5 methoxyphenylhydrazine (820 mg, 4.7 mmol) in absolute ethanol (50 mL), DIEA (1 g,) and acetic acid (0.5 mL) was added and the mixture refluxed for 1h. After removing the solvent, the residue obtained was taken as such for the next step. The crude product from the previous reaction was taken in formic acid (50 mL) and heated at 100 'C for one hour. After which it was poured into water and made basic by 10 adding aqueous sodium hydroxide and then extracted with DCM. The crude 9-methoxy 1,2,3,4,5,6-hexahydroazepino[4,5-b]indole obtained was taken as such for the next step. To Example 7 (lg, 4.3 mmol) in acetonitrile (50 mL), crude 9-methoxy-1,2,3,4,5,6 hexahydroazepino[4,5-b]indole (1g, 4.6 mmol) and potassium carbonate (1g, 7.2 mmol) was added and the mixture refluxed for 4h. The crude product obtained on removal of solvent 15 was chromatographed on silica gel column by using 50% ethyl acetate in hexanes followed by re-crystallization from hexane and DCM (1:1) resulted in pure methyl-2-(9-methoxy 1,4,5,6-tetrahydroazepino[4,5-b]indol-3(2H)-1-yl)pyrimidine-5-carboxylate (200 mg, 13.3%); m/e = 353 (M+1). To the methyl-2-(9-methoxy-1,4,5,6-tetrahydroazepino[4,5-b]indol-3(2B)-1 20 yl)pyrimidine-5-carboxylate (200 mg, 0.56 mmol) in methanol (10 mL) and DCM (10 mL), 50% aqueous hydroxylamine (2 mL) and aqueous sodium hydroxide (160 mg, 4 mmol) was added and stirred at room temperature for 4 h. After removing the solvent, the residue was dissolved in water (10 mL) and acidified with concentrated hydrochloric acid. The white solid precipitated was filtered, washed with water (30 mL), ether (20 mL) and dried under 25 vacuum to give Example 113 (180 mg, 90%). 1H NMR (300 MHz, DMSO-d 6 ) 8:2.91-3.01 (in, 4H), 3.73 (s, 3H), 4.07 (in, 4H), 6.60 (in, 1H), 6.88 (s, 1H), 7.08 (in, 1H), 8.69 (m, 2H), 166 WO 2006/088949 PCT/US2006/005312 8.98 (s, 1H), 10.57 (s, 1H), 11.03 (s, 1H); m/e = 354 (M+1). 0 N N NHOH aNN N H Example 114. N-hydroxy-2-(1,4,5,6-tetrahydroazepino[4,5-blindol-3(2H)-1 5 yl)pyrimidine-5-carboxamide Example 114 was prepared in the same manner as Example 113 by substituting phenylhydrazine for 4-methoxyphenylhydrazine in the reaction sequence. A solid; 1H NMR (DMSO-d 6 , 300 MHz) 6; 2.94-3.05 (m, 4H, 4.07 (m, 4H), 6.94 (m, 2H), 7.21 (d, J= 7.8 Hz, iH), 7.38 (d, J= 7.5 Hz, 1H), 8.98 (bs, 1H), 10.74 (s, 1H), 11.03 (s, 1H); m/e = 322 (M+1). 0 N NHOH F N N N H 10 Example 115. N-hydroxy-2-(9-fluoro-1,4,5,6-tetrahydroazepino[4,5-blindol-3(2H)-1 yl)pyrimidine-5-carboxamide Example 115 was prepared in the same manner as Example 113 by substituting 4 fluorophenylhydrazine for 4-methoxyphenylhydrazine in the reaction sequence. A solid; 1H 15 NMR (DMSO-d6, 300 MHz) 8; 2.90 (t, J= 5 Hz, 2H), 3.03 (t, J= 5 Hz, 2H), 4.06 (m, 411), 6.79 (t, J= 9 Hz, 1H), 7.16 (m, 2H), 8.69 (s, 2H), 8.98 (s, 1H), 10.86 (s, 1H), 11.03 (s, 1H); m/e = 340 (M+1). 167 WO 2006/088949 PCT/US2006/005312 0 N N NHOH N N NN c) H Example 116. N-hydroxy-2-(9-[3-(morpholin-4-ylmethy)phenyl]-1,4,5,6 tetrahydroazepino[4,5-blindol-3(2H)-1-yl)pyrimidine-5-carboxamide Methyl-2-(9-bromo-1,4,5,6-tetrahydroazepino[ 4 ,5-b]indol-3(2H)-1-yl)pyrimidine-5 5 carboxylate was obtained by substituting 4-bromophenylhydrazine for 4 methoxyphenylhydrazine in the reaction sequence used to make methyl-2-(9-methoxy 1,4,5,6-tetrahydroazepino[4,5-b]indol-3(2H)-1-yl)pyrimidine-5-carboxylate in Example 113. To a solution of methyl-2-(9-bromo-1,4,5,6-tetrahydroazepino[ 4 ,5-blindol-3(2H)-1 yl)pyrimidine-5-carboxylate (1.9g, 4.73 mmol) in THF:H 2 0 (4:1) were added Cs 2

CO

3 10 (12.33g, 37.90 mmol) and 3-formylphenylboronic acid (1.41g, 9.46 mmol). The reaction mixture was thoroughly degassed and freshly prepared tetrakis(triphenylphosphine)palladium(O) (1.36g, 1.18 mmol) was added under nitrogen atmosphere at room temperature. The reaction mixture temperature was raised to 80 0 C and continued stirring for 12hr. The progress of the reaction was monitored by TLC and upon 15 completion of the reaction the THF was removed under reduced pressure. The mixture was partitioned between ethyl acetate (100 mL) and water (15 mL). The organic layer was separated, dried over Na 2 SO4 filtered and the solvent was removed under reduced pressure to give crude residue, which was purified by column chromatography using silica gel to provide methyl-2-(9-[3-(formyl)phenyl]-1,4,5,6-tetrahydroazepino[ 4 ,5-b]indol-3(2H)-1 20 yl)pyrimidine-5-carboxylate (0.9g, 44.7%) To a 0 (C solution of methyl-2-( 9

-[

3 -(formyl)phenyll-1,4,5,6-tetrahydroazepino[4,5 blindol-3(2H)-1-y1)pyrimidine-5-carboxylate (0.15g, 0.35 mmol) in DCM (20mL) was added Na(OAc)3BH (0.149g, 0.70 mmol) and stirred for 10 min. To the reaction mixture was added morpholine (0.153g, 1.76 mmol) under nitrogen atmosphere and the reaction 25 mixture was continued stirring at room temperature for 4hr. The progress of the reaction was monitored by TLC and upon completion of the reaction the mixture was partitioned 168 WO 2006/088949 PCT/US2006/005312 between DCM (50 mL) and water (25 mL) and the organic layer was separated washed with water (2 x 25 mL), dried over sodium sulfate, filtered and the solvent was removed under reduced pressure to give crude residue, which was purified by column chromatography using silica gel to provide methyl-2-(9-[3-(morpholin-4-ylmethyl)phenyl]-1,4,5,6 5 tetrahydroazepino[4,5-b]indol-3(2H)-1-yl)pyrimidine-5-carboxylate (0.2g). To a 0 *C solution of methyl-2-(9-[3-(morpholin-4-ylmethyl)phenyl]-1,4,5,6 tetrahydroazepino[4,5-b]indol-3(2H)-1-yl)pyrimidine-5-carboxylate (0.2g) in methanol: DCM (10ml:4ml) was added 50% aqueous hydroxylamine solution (4 mL) and to the mixture was added a solution of NaOH (0.12g) in water (1 mL). The reaction mixture was 10 stirred at room temperature for lhr and the progress of the reaction was monitored by TLC analysis and upon completion of the reaction the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using 1N HCl and the obtained solid was filtered and washed with water followed by diethyl ether and dried under vacuum to give Example 116; HPLC: (Rt = 12.39); m/e = 499 (M+1). 15 0 N NHOH N N N NN 0 H Example 117. N-hydroxy-2-(9-[3-((4-methylpiperazin-1-yl)methy)phenyll-1,4,5,6 tetrahydroazepino[4,5-b]indol-3(2H)-1-yl)pyrimidine-5-carboxamide Example 117 was synthesized in the same manner as Example 116 by substituting 20 N-methylpiperazine for morpholine in the reaction sequence. A solid, HPLC: (Rt = 11.81 min); m/e = 512 (M+1). 169 WO 2006/088949 PCT/US2006/005312 0 N ~ NHOH N N Example 118. 2-(3,4-dihydro[1]benzothieno[2,3-clpyridin-2(1H)-yl)-N hydroxypyrimidine-5-carboxamide 1,2,3,4-Tetrahydro[1]benzothieno[2,3-c]pyridine was prepared as described by 5 Wolf, G.; and Zymalkowski, F.; Arch. Pharm. 1976 (Weinheim Ger.), 309, 279-288. Hydrochloric acid was bubbled vigorously through a mixture of benzothiophene (5.0 g, 37.2 mmol), 37% aqueous formaldehyde (4.4 mL) and concentrated HCl (4.4 mL) until the reaction temperature rose to 65 0 C. At this time the flow of HCl gas was reduced to a slow stream and maintained for 1.5 h. The reaction mixture was diluted with water (10 mL) and 10 subsequently extracted with ether (2 x 25 mL). The combined organic extracts were dried over anhydrous Na 2

SO

4 and concentrated under reduced pressure and the residue was purified by silica gel column chromatography using ethyl acetate - hexane to furnish 3 (chloromethyl)-1-benzothiophene (4.5 g, 67%) ; 'H NMR (200 MHz, CDCl 3 ): S 4.90 (s, 2H), 7.25 (s, 1H), 7.45 (in, 2H), 7.88 (in, 2H). 15 To a stirred solution of NaCN (1.4 g , 28.5 mmol) in DMSO (8 mL) was added 3 (chloromethyl)-1-benzothiophene (4 gm, 21.9 mmol) in DMSO (6 mL) over 15 min. The reaction mixture was stirred at room temperature for 18 h and then quenched with water and extracted with ether (2 x 50 mL). The organic layer was washed with brine, dried over anhydrous Na 2

SO

4 , filtered and concentrated under reduced pressure. The residue obtained 20 was purified by silica gel column chromatography using ethyl acetate - hexane (1:1) to furnish 1-benzothien-3-ylacetonitrile (2.5 g, 65%). 'H NMR (200 MHz, CDCl 3 ): S 3.69 (s, 2H), 7.25 - 7.91 (in, 5H). To a slurry of lithium aluminium hydride (0.6 g, 15.16 mmol) in ether (25 mL) was added, under N 2 , a slurry of aluminium chloride (2.lg, 15.1 mmol) in dry ether. After 5 25 min, a solution of 1-benzothien-3-ylacetonitrile (2.5, 14.4 mmol) in ether (25 mL) was slowly added over 10 min .Upon completion of the addition, the resulting reaction mixture was refluxed for 18 h, cooled and neutralized with 6N NaOH and extracted with ethyl 170 WO 2006/088949 PCT/US2006/005312 acetate (2 x 50 mL). The combined organic extracts were dried over anhydrous Na 2 SO4, filtered and concentrated under reduced pressure to yield a syrup. 2-(1 -Benzothien- 3 yl)ethanamine hydrochloride was obtained by treating the syrup with a solution of methanolic HCI (1.5 g, 50%); IH NMR (200 MHz, CDCl 3 ): 8 1.43 (s, 2H), 3.00 (in, 4H), 5 7.11 (s, 1H), 7.36 (in, 2H), 7.72-7.86 (in, 2H). A mixture of 2-(benzo[b]thien-3-yl)ethylamine hydrochloride (0.5 g, 2.34 mmol) and paraformaldehyde (0.125 g, 4.16 mmol) in methanol (15 mL) was refluxed for 24 h. The reaction mixture was concentrated under reduce pressure and the residue was neutralized with saturated NaHCO3 (50 mL), and extracted with ethyl acetate (2 x 50 mL). 10 The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated to furnish 1,2,3,4-tetrahydro[1]benzothieno[2,3-c]pyridine (160mg, 35%); 'H NMR (200 MHz, CDCl 3 ): 8 2.81( m, 2H), 3.25 (in, 2H), 4.15 (s,2H), 7.30 (m, 2H), 7.69 (d, J= 4.0 Hz, 1H), 7.79 ((d, J= 4.0 Hz, 1H). A mixture of 1,2,3,4-tetrahydro[1]benzothieno[2,3-c]pyridine ( 0.160 g, 0.855 15 mmol), Example 7 (0.280 g, 1.3 mmol) and K 2 C0 3 (0.6 g, 4.27 mmol) in DMF (10 niL) was stirred at 90 0 C for 3 h. The reaction mixture was cooled to room temperature, quenched by addition of water (50 mL) and extracted with ethyl acetate (2x 25 mL). The organic layer was washed with water and brine , dried over anhydrous Na 2 SO4, filtered and concentrated under reduced pressure and the residue was purified by silica gel column 20 chromatography using ethyl acetate-hexane (1:1) to furnish methyl-2-( 3

,

4 dihydro[1]benzothieno[2,3-c pyridin-2(1H)-y1)-pyrimidine-5-carboxylate (0.08 g, 50%). 1H NMR (200 MHz, CDCl 3 ): 6 2.99 (in, 2H), 3.89 (s, 3H), 4.35 (m,2H), 5.20 (s,2H), 7.35 (m, 2H), 7.61 (d, J= 4.0 Hz, 1H), 7.81 ((d, J= 4.0 Hz, 1H), 8.91(s,2H). To a cooled (0 C) solution of methyl-2-( 3 ,4-dihydro[1 ]benzothieno[ 2

,

3 -c]pyridin 25 2 (1H)-y)-pyrimidine-5-carboxylate (0.080 g, 0.28 mmol) in methanol: DCM (6 mL, 2:1) was added aqueous 50 % NH 2 OH solution (2 mL) and NaOH (0.08 g, 2.0 mmol) dissolved in 0.5 ml of water. The reaction mixture was stirred for 3 h at room temperature and the reaction mixture was concentrated under reduced pressure to give crude residue. The residue was neutralized with 2N HC1 and the precipitated solid was filtered and dried to 30 give pure Example 118 (0.030 g, 38%). 1H NMR (200 MHz, CD 3 0D): 6 2.97 (in, 2H), 4.36 171 WO 2006/088949 PCT/US2006/005312 (m,2H), 5.20 (s,2H), 7.29 (in, 2H), 7.64 (d, J= 4.0 Hz, 1H), 7.84 (d, J= 4.0 Hz, 1H), 8.95(s,2H); m/e = 327 (M+1). 0 N ~ NHOH N N 5 Example 119. 2-(3,4-dihydro[1]benzofuro[2,3-c]pyridin-2(1H)-yl)-N hydroxypyrimidine-5-carboxamide 1,2,3,4-Tetrahydro[ 1]benzofuro [2,3-c]pyridine was prepared by the method described by Jaen, J. and Wise, L. D.; J' Heterocycl. Chem. 1987, 1317-1319. To a reaction mixture containing Mg turnings (0.85, 35.41 mmol), a pinch of iodine in THF (5 mL) 10 under N 2 atmosphere was added a mixture of 2-bromoanisole ( 5.0 g, 26.7 mmol) and 1,2 dibromoethane(1.4 g, 8.8 mmol) in (10 mL) THF. When all the magnesium was consumed, a solution of 1-benzyl-4- piperidone (5.0 g, 28.24 mmol) in THF (10 mL) was added dropwise. Following the addition, the solution was refluxed for 20 minutes, and then stirred at room temperature for 2 h. The reaction mixture cooled to 0 C and 10% HCl was added 15 dropwise until the pH of the mixture was 1-2. The pH was adjusted to 10 with 2N NaOH and extracted with ether (2 x 100 mL). The organic extracts were dried over anhydrous Na 2

SO

4 ,filtered and concentrated under reduced pressure to furnish 1-benzyl-4-(2 methoxyphenyl)piperidin-4-ol (4.5 g, 90%). 'H NMR (200 MHz, CDCl 3 ): 6 2.20 (d, J=17 Hz, 2H), 2.60-3.15 (in, 2H), 3.20-3.50 (in, 4H), 3.90 (s, 3H), 4.20 (d, J=7 Hz, 2H),6.90 20 7.05 (m,2H), 7.20-7.35 (in, 2H), 7.35-7.55 (m, 3H), 7.60-7.80(m, 2H); m/e = 297. 1-Benzyl-4-(2-methoxyphenyl)piperidin-4-ol (4.5 g, 15.15 mmol) was mixed with anhydrous potassium bisulphate (8.0 g, 58.56 mmol) and heated to 160 C under vacuum at 10mm Hg for 0.5 h. The flask was cooled, the contents dissolved in water and the solution was saturated with sodium carbonate and extracted with ether ( 2 x 100 mL). The organic 25 layer was washed with brine, dried over anhydrous Na 2 S04, filtered and concentrated under reduced pressure and the residue was purified by silica gel column chromatography using ethyl acetate-hexane (1:1) to furnish 1 -benzyl-4-(2-methoxyphenyl)- 1,2,3,6 172 WO 2006/088949 PCT/US2006/005312 tetrahydropyridine (2.0 g,45%). 'H NMR (200 MHz, CDCl 3 ): 8 2.551-2.573 (in, 2H), 2.67 2.71 (in, 2H), 3.16-3.19 (brs, 2H), 3.66 (s, 2H), 3.79 (s, 3H), 5.76-5.78 (brs, 1H), 6.83 - 6.92 (in, 2H), 7.15-7.41 (in, 7H); m/e = 279 (M+. Sodium borohydride (0.590 g, 15.94 mmol) was added at once to a solution of 1 5 benzyl-4-(2-methoxyphenyl)-1,2,3,6-tetrahydropyridine (2.0 g, 7.16 mmol) in (7 mL) anhydrous diglyme at 0 0 C. The reaction mixture was warmed to room temperature and a solution of borontrifluoride etherate (2.0 g 14.2 mmol) in (2 mL) diglyme was added dropwise under a N 2 . The reaction mixture was stirred at room temperature for 2 h. Water (0.8 mL) was then added to the reaction mixture slowly followed by 6N NaOH (1.8 mL). 10 The reaction mixture was heated to 50 "C for another 45 min. and then 30% H 2 0 2 (1.7 mL, 14.7 mmol) was added. The reaction mixture was stirred for another 45 min., Concentrated HCl (1.7 mL) was added and the solvents were evaporated in vacuum. Water (7 mL) was added to the residue and the solvent again evaporation. The residue was quenched with saturated ammonium hydroxide solution and extracted with DCM (2 x 50 mL). The organic 15 layer was dried over anhydrous Na 2

SO

4 , filtered and concentrated under reduced pressure to, give 1-benzyl-4-(2-methoxyphenyl)piperidin-3-ol (1.0 g, 50 %); 'H NMR (200 MHz, CDCl 3 ): 8 1.79-2.18 (in, 5H), 2.91-3.045 (in, 2H), 3.184-3.256 (dd, J=10.4, 4.0 Hz, 2H), 3.615 (s, 2H), 3.827 (s, 3H), 3.785-3.949 (in, 1H), 6.892 (d, J=8.5 Hz, 2H), 6.974 (t, J=7.5 Hz, 1H), 7.188-7.416 (in, 7H); m/e = 297 (M+1). 20 To a cooled (-78 * C) solution of oxalyl chloride (0.839 g, 6.73 mmol) in dry DCM (5 mL) was added dimethylsulfoxide (1.05 g, 13.46 mmol) dropwise. The mixture was stirred for 10 min at -78 0 C, 1-benzyl-4-(2-methoxyphenyl)piperidin-3-ol (1.0 g, 3.367 mmol) in DCM (10 mL) was added and stirring continued for a further 20 min at -78 C. Triethylamine (3.3 ml, 23.56 mmol) was added, the reaction mixture was stirred for 15 min 25 at -78 "C, warmed to room temperature and diluted with water. The organic layer washed with 2N HCl (50 mL), 10% aqueous sodium bicarbonate solution, brine and dried over anhydrous Na 2

SO

4 , filtered and concentrated under reduced pressure to afford 1-benzyl-4 (2-methoxyphenyl)piperidin-3-one (0.7 g, 70%) which was used in the next step without further purification. 'H NMR (200 MHz, CDCl 3 ): 6 2.72(m, 2H ) 2.91-3.045 (in, 2H), 30 3.71(m, 2H), 3.80 (s, 2H), 6.87-6.99 (in, 2H), 7.12 (dd, J=7.5, 1.7 Hz, 1H), 7.18-7.45 (in, 173 WO 2006/088949 PCT/US2006/005312 6H); m/e = 295 (M+1). The crude 1-benzyl-4-(2-methoxyphenyl)piperidin-3-one (0.7 g, 2.37 mmol) obtained in the previous step was dissolved in 4 mL of glacial acetic acid. To this solution, 48% hydrobromic acid (4 mL) was added, and the mixture was refluxed under N 2 for 4 h. 5 After cooling to room temperature, the reaction mixture was poured over ice cold ethyl acetate (50 mL) and concentrated ammonium hydroxide solution (50 mL). The organic phase was washed with water, brine, dried over anhydrous Na 2

SO

4 , filtered and concentrated under reduced pressure and the residue was purified by silica gel column chromatography to give 2-benzyl-1,2,3,4-tetrahydro[1]benzofuro[2,3-c]pyridine (200 mg, 10 33%). 'H NMR (200 MHz, CDCl 3 ): 8 2.70-2.76 (in, 2H) 2.85-2.91 (in, 2H), 3.67 (t, J= 1.8 Hz, 2H), 3.78 (s, 2H), 7.18-7.45 (in, 9H); m/e = 263 (M*). A mixture of 2-benzyl-1,2,3,4-tetrahydro[1]benzofuro[2,3-c]pyridine (0.2 g, 0.75 mmol) and methyl chloroformate (0.358 g, 3.787 mmol) in dichloroethane (15 mL) was refluxed for lh . The reaction mixture was cooled to room temperature and washed with 15 saturated NaHCO 3 (25 mL). The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated to furnish methyl 3,4-dihydro[1]benzofuro[2,3-c]pyridine-2(lH) carboxylate (0.150 g, 85.7%). 1H NMR (200 MHz, CDCl 3 ): 8 2.70-2.76 (in, 2H), 3.80 (s, 5H), 4.72 (in, 2H), 7.22 (in, 2H), 7.42(m, 2H). A mixture of methyl 3,4-dihydro[1]benzofuro[2,3-c]pyridine-2(1H)-carboxylate 20 (0.150 g, 0.6493 mmol) and concentrated HCl (10 ml) was refluxed for 18 h . The reaction mixture was cooled to 0 0 C, quenched with saturated aqueous NaHCO 3 (100 mL), and extracted with ethyl acetate ( 2 x 50mL), the organic layer was dried over anhydrous Na 2

SO

4 , filtered and concentrated under reduced pressure to furnish 1,2,3,4 tetrahydro[l]benzofuro[2,3-c]pyridine (0.1 g , 46 %) . H NMR (200 MHz, CDCl 3 ): 8 3.15 25 (m, 4H ), 4.40 (in, 2H), 7.35 (m,2H), 7.5 1(m, 2H). MS: 160 (M). A mixture of 1,2,3,4-tetrahydro[1]benzofuro[2,3-c]pyridine ( 0.1 g, 0.432 mmol), Example 7 (0.112 g, 0.519 mmol) and K 2 C0 3 (0.298 g, 2.164 mmol) in DMF (5 mL) was stirred at 90 "C for 3 h. The reaction mixture was cooled to room temperature, quenched by addition of water (50 mL) and extracted with ethyl acetate (2x 25 mL). The organic 30 layer was washed with water and brine, dried over anhydrous Na 2 S04, filtered and 174 WO 2006/088949 PCT/US2006/005312 concentrated under reduced pressure and the residue was purified by silica gel column chromatography using ethyl acetate-hexane (1:1) to furnish methyl-2-(3, 4 dihydro[1]benzofuro[2, 3 -c]pyridin- 2 (1H)-yl)-pyrimidine-5-carboxylate (0.08 g, 43%). 'H NMR (200 MHz, CDCl 3 ): 6 2.82 (m, 2H), 3.90 (s, 3H), 4.31 (in, 2H), 5.09 (in, 2H) , 7.52 5 (m, 4H), 8.91(s, 2H). To a cooled (0 0 C) solution of methyl- 2

-(

3 ,4-dihydro[1]benzofuro[2,3-c]pyridin 2 (I1H)-yl)-pyrimidine-5-carboxylate (0.08 g, 0.2179 mmol) in methanol: DCM (6 ml, 2:1) was added 50 % aqueous hydroxylamine (2 mL) and NaOH (0.08 g, 2.0 mmol) dissolved in 0.5 ml of water. The reaction mixture was stirred for 3 h at room temperature and the 10 reaction mixture was concentrated under reduced pressure to give crude residue. The residue was neutralized with 2N HCl until the pH is neutral, the precipitated solid was filtered and dried to give Example 119 (0.040 g, 50%). 'H NMR (200 MHz, CD 3 0D): 6 2.85 (m, 2H), 4.32 (in, 2H), 5.05 (s, 2H), 7.30 (in, 2H), 7.51(m, 2H), 8.79(s, 2H); m/e = 311 (M+1). 15 0 N ~ NHOH NN H Example 120. N-hydroxy-2-{7-phenyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl}pyrimidine-5-carboxamide Methyl-2-(7-bromo-1,3, 4 ,5-tetrahydro-2H-pyrido4,3-bindol-2-yl)pyrimidine-5 20 carboxylate was prepared in the same manner as Example 33 by substituting 3 bromophenylhydrazine for 4-bromophenylhydrazine. To a solution of the ester (1.0g, 2.58 mmol) in THF:H20 (4:1) were added Cs 2 CO3 (6.71g, 20.65 mmol) and phenylboronic acid (0.629g, 5.16 mmol). The reaction mixture was thoroughly degassed and freshly prepared tetrakis(triphenylphosphine)palladium(0) (1.491 g, 1.29 mmol) was added under nitrogen 25 atmosphere at room temperature. The reaction mixture temperature was raised to 80 0 C and continued stirring for 12hr. The progress of the reaction was monitored by TLC analysis and upon completion of the reaction the THF was removed under reduced pressure. The mixture 175 WO 2006/088949 PCT/US2006/005312 was partitioned between ethyl acetate (100 mL) and water (15 mL). The organic layer was separated, dried over Na 2

SO

4 filtered and the solvent was removed under reduced pressure to give crude residue, which was purified by column chromatography using silica gel to provide methyl-2-{7-phenyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5 5 carboxylate (0.1g, 10.09%). To a 0 'C solution of methyl-2-{7-phenyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol 2-yl}pyrimidine-5-carboxylate (0.1g) in methanol :DCM (5:2ml) was added 50% aqueous hydroxylamine solution (2ml) and to the mixture was added solution of NaOH (0.08g) in water(0.5 mL). The reaction mixture was stirred at room temperature for lhr and the 10 progress of the reaction was monitored by TLC and upon completion of the reaction the solvent was removed under reduced pressure. The pH of the mixture was adjusted to 7.5 using 1N HCl and the obtained solid was filtered and washed with water followed by diethyl ether and dried under vacuum to give Example 120 (0.025g, 24.91%). HPLC: (Rt = 14.77). 'HNMR (DMSO-d 6 , 200 MHz) 8: 11.03 (s, 1H), 8.72 (s, 2H), 7.62 (d, 2H), 7.56 15 (d.2H), 7.43 (in, 2H), 7.28 (d, 2H), 4.98 (s, 2H), 4.25 (in, 2H), 2.90 (in, 2H); m/e = 386 (M+1). 0O N - NHOH N H Example 121. N-hydroxy-2-{7-[3-(pyrrolidin-1-ylmethyl)phenyl]-1,3,4,5-tetrahydro 20 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide To a solution of methyl-2-(7-bromo-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl)pyrimidine-5-carboxylate (0.360g, 0.93 mmol) in THF:H 2 0 (4:1) were added Cs 2

CO

3 (2.42g, 7.44 mmol) and 3-fornylphenylboronic acid (0.27g, 1.86 mmol). The reaction mixture was thoroughly degassed and freshly prepared 25 tetrakis(triphenylphosphine)palladium(0) (0.268g, 0.23 mmol) was added under nitrogen atmosphere at room temperature. The reaction mixture temperature was raised to 80 'C and 176 WO 2006/088949 PCT/US2006/005312 continued stirring for 12hr. The progress of the reaction was monitored by TLC and upon completion of the reaction the THF was removed under reduced pressure. The mixture was partitioned between ethyl acetate (100 ml) and water (15 mL). The organic layer was separated, dried over Na 2 SO4 filtered and the solvent was removed under reduced pressure 5 to give crude residue, which was purified by column chromatography using silica gel to provide methyl-2-{7-[3-(formyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[ 4

,

3 -b]indol-2 yl}pyrimidine-5-carboxylate (0.15g, 39.13%). To a 0 0 C solution of methyl- 2 -{7-[3-(formyl)phenyl1-1,3,4,5-tetrahydro- 2

H

pyrido[ 4

,

3 -b]indo1-2-yl}pyrimidine-5-carboxylate (0.15g, 0.36 mmol) in DCM (30 mL) was 10 added Na(OAc) 3 BH (0.154g, 0.73 mmol) and stirred for 10 min. To the reaction mixture was added pyrrolidine (0.129g, 1.82 mmol) under nitrogen atmosphere and the reaction mixture was continued stirring at room temperature for 4hr. The progress of the reaction was monitored by TLC analysis and upon completion of the reaction the mixture was partitioned between DCM (50 mL) and water (25 mL) and the organic layer was separated, 15 washed with water (2 x 25 mL), dried over sodium sulfate filtered and the solvent was removed under reduced pressure to give a crude residue, which was purified by column chromatography using silica gel to provide methyl-2-{7-[-(pyrrolidin-1-ylmethyl)phenyl) 1,3,4,5-tetrahydro-2H-pyrido(4,3-b]indol-2-y1}pyrimidine-5-carboxylate (0.06g, 35.39%). To a 0 "C solution of methyl-2-{7-[3-(pyrrolidin-1-ylmethy1)phenyll-1,3,4,5 20 tetrahydro-2H-pyrido[ 4

,

3 -blindol-2-y1}pyrimidine-5-carboxylate (0.05g) in methanol :DCM (5:2mL) was added 50% aqueous hydroxylamine solution (lmL) and to the mixture was added solution of NaOH (0.04g) in water (0.25 mL). The reaction mixture was stirred at room temperature for Ihr and the progress of the reaction was monitored by TLC analysis and upon completion of the reaction the solvent was removed under reduced pressure. The 25 pH of the mixture was adjusted to 7.5 using IN HCl and the obtained solid was filtered and washed with water followed by diethyl ether and dried under vacuum to give Example 121 (0.005g). HPLC (Rt = 12.65); IHNMR (DMSO-d6, 200 MHz) 8: 11.017 (s, 1H), 8.73 (s, 2H), 7.23-7.56 (in, 8H), 4.99 (s, 2H), 4.25 (in, 2H), 3.63 (s, 2H), 2.90 (in, 2H), 2.49 (in, 4H), 1.69 (in, 4H); m/e = 468 (M+1). 30 177 WO 2006/088949 PCT/US2006/005312 0 NK N NHOH H Example 122. N-hydroxy-2-(10-oxo-3,4,5,10-tetrahydrobenzo[b]-1,6-naphthyridin 2(1H)-yl)pyrimidine-5-carboxamide 1,3,4,5-Tetrahydrobenzo[b]-1,6-naphthyridin-10(2H)-one was prepared as described 5 inJ Am. Chem. Soc. 1959, 81, 3098-3100. Further reaction of 1,3,4,5-tetrahydrobenzo[b] 1,6-naphthyridin-10(2H)-one with Example 7 and subsequent conversions as in Example 12 gave Example 122 as a yellow powder after hplc purification. 1H NMR (300MHz, DMSO d 6 ) 8: 2.90 (m, 2H), 4.15 (m, 2H), 4.68 (s, 2H), 7.26 (t, 1H), 7.49 (d, 1H), 7.61 (t, 1H), 8.09 (d, 1H), 8.72 (s, 2H), 9.0 (bs, 1H), 11.08 (s, 1H), 11.69 (s, 1H); m/e = 338 (M + 1). 10 0 -N N N NHOH NIN N H Example 123. N-hydroxy-2-{8-[(4-methylpiperazin-1-yl)methyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide To a 0 0 C cooled solution of 4-nitrobenzaldehyde in DCM (40 mL) was added 15 Na(OAc) 3 BH (10.526gm, 49.66 mmol) and the reacton was stirred for 10min. To the reaction mixture was added N-methylpiperazine (9.93 g, 99.3 mmol) under nitrogen atmosphere and the reaction mixture was continued stirring at room temperature for 4h. The progress of the reaction was monitored by TLC and upon completion of the reaction, the mixture was partitioned between DCM (20 mL) and water (15 mL) and the organic layer 20 was separated, washed with water (2 x 15 mL), dried over sodium sulphate, filtered and the solvent was removed under reduced pressure to give crude residue. Washing with ether gave 1-methyl-4-(4-nitrobenzyl)piperazine (4 g). 1H NMR (CDCl 3 , 200MHz) 5: 8.19 (2H, d, J= 8.4Hz), 7.53 (2H, d, J= 8.4 Hz), 3.59 (2H, s), 2.47 (8H, bm), 2.29 (3H, s); m/e = 236 (M+1). 178 WO 2006/088949 PCT/US2006/005312 To a solution of 1-methyl-4-(4-nitrobenzyl)piperazine (4.0 g) in methanol (100 mL) at room temperature under nitrogen atmosphere was added Raney nickel (1.6gm). The reaction mixture was stirred for 2 hr under hydrogen atmosphere. The progress of the reaction was monitored by TLC and upon completion of the reaction, the mixture was 5 filtered under nitrogen atmosphere and the solvent was removed under reduced pressure to give 4 -[(4-methylpiperazin-1-yl)methyllaniline (3.2 g). 'H NMR (CDCl3 , 200MHz) 8: 7.13 (2H, d, J= 8.4 Hz), 6.61 (2H, d, J= 8.4 Hz), 3.41 (2H, s), 2.45 (8H, bm), 2.27 (3H, s); m/e =206 (M+1). To a stirred solution of 4-[(4-methylpiperazin-1-yl)methyl]aniline (3.2 g, 15.57 10 mmol) in acetic acid: concentrated HCl (32:32 mL) at 10 'C was added NaNO2 (1.30 g, 18.78 mmol) in water (16 mL) and stirred for 10 min. Freshly prepared SnCl 2 .2H20 (11.75 g, 51.97 mmol) in concentrated HCl (32 mL) was added at 10 0 C. The temperature of the reaction mixture was allowed to rise to room temperature and maintained there for 4hr. After filtering the reaction mixture, the precipitate was washed with water and the solid 15 obtained was dried under reduced pressure to obtain 1-(4-hydrazinobenzyl)- 4 methylpiperazine (3.4 g). 1 H NMR (CD 3 0D, 200MHz) 8: 7.66 (2H, d, J= 8.4 Hz), 7.13 (2H, d, J= 8.4 Hz), 4.46 (2H, s), 3.72 (8H, bm), 3.11 (3H, s); To a solution of 1

-(

4 -hydrazinobenzyl)-4-methylpiperazine (3.4 g, 13.25 mmol) in ethanol (50 mL) were added piperidone. HCl (2.51 g, 18.55 mmol). The reaction 20 temperature was raised to 90 0 C and continued stirring for 2 hrs. The progress of the reaction was monitored by TLC and upon completion of the reaction the mixture was cooled to rt and HCI gas was bubbled through the reaction mixture at 0 'C. After the reaction mixture was saturated with HCI, the temperature was raised to 90 0 C again and continued stirring for 2hrs. The ethanolic HCl was removed under reduced pressure and the 25 pH of the reaction mixture was adjusted to 12.0 with 10% NaOH solution. The mixture was partitioned between 20% MeOH: DCM and water (35 mL) and the organic layer was separated, dried over Na 2 SO4 filtered and the solvent was removed under reduced pressure to give crude residue. Washing with ether gave 8-[(4-methylpiperazin-1-yl)methy1]-2,3,4,5 tetrahydro-1H-pyrido[4,3-b]indole (1 g). 1 HNMR

(CD

3 0D, 200MHz) 8: 7.31 (1H, s), 7.27 30 (1H, d, J= 8.6 Hz), 7.06 (1H, d, J= 8.6 Hz), 4.01 (2H, s), 3.60 (2H, s), 3.21 (8H, bm), 2.86 179 WO 2006/088949 PCT/US2006/005312 (4H, in), 2.28 (3H, s); m/e = 285 (M+1). To a solution of 8-[(4-methylpiPerazin-1 -yl)methyl] -2,3,4,5-tetrahydro-1H pyrido[4,3-b]indole(O.5 g,1.

7 6 mmol) in DMF (15 mL) at rt was added Example 7 (0.567 g, 2.64 mmol) and K 2 C0 3 (0.731 g, 5.28 mmol). The reaction temperature was raised to 100 5 (C and continued stirring for 12hr. The progress of the reaction was monitored by TLC and upon completion of the reaction DMF was removed under reduced pressure. The reaction mixture was partitioned between ethyl acetate (100 mL) and water (80 mL) and the organic layer was separated, dried over sodium sulphate, filtered and the solvent was removed under reduced pressure to give crude residue. Washing with ether gave methyl 2-{8-[(4 10 methylpiperazin-1-yl)methyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5 carboxylate (0.15 g). 'HNMR (DMSO-D6, 200MHz) 8: 8.84 (2H, s), 7.94 (1H,s), 7.67 (1H, d, J= 8.6 Hz), 7.54 (1H, d, J= 8.6 Hz), 5.01 (2H, s), 3.80-3.01 (10H, m), 2.87 (3H, s); m/e = 421 (M+1). To a 0 0 C solution of methyl 2 -{8-[(4-methylpiperazin-1-yl)methyl]-1,3,4,5 15 tetrahydro-2H-pyrido[ 4

,

3 -b]indol-2-yl}pyrimidine-5-carboxylate (0.1 g) in MeOH: DCM (5:2 mL) was added 50% aqueous hydroxylamine solution (2 mL) and to the mixture was added a solution of NaOH (0.08 g) in water (1 mL). The reaction mixture was stirred at room temperature for lhr and the progress of the reaction was monitored by TLC and upon completion of the reaction the solvent was removed under reduced pressure. The pH of the 20 mixture was adjusted to 7.5 using IN HCl and the obtained solid was filtered and washed with water followed by diethyl ether. After filtering, the solid was dried under vacuum to give N-hydroxy-2-{8-[(4-methylpiperazin-1-yl)methyl]-1,3,4,5-tetrahydro-2H-pyrido[ 4

,

3 b]indol-2-yl}pyrimidine-5-carboxamide (0.075g). 1H NMR (DMSO-D6, 200MHz) 6: 10.91 (2H, bm), 8.74 (21, s), 7.64-7.01 (3H, in), 4.91 (2H, s), 4.19 (2H, s), 3.50-2.88 (10H, in), 25 2.87 (3H, s); m/e = 422.9 (M+1). BIOLOGICAL EXAMPLES Example A In vitro fluorescent histone deacetylase assay Histone deacetylase (HDAC) activity assays were performed using the HDAC 180 WO 2006/088949 PCT/US2006/005312 fluorescent activity assay/drug discovery kit (Biomol Research Laboratories, Plymouth Meeting, PA) essentially according to the manufacturer's instructions. The included HeLa cell nuclear extract, which contains a mosaic of HDAC enzymes and other nuclear factors, was used as the source of HDAC activity. The final substrate concentration in the assay 5 mixture was 50 pM. The reaction was allowed to proceed for 10 min at room temperature before stopping the reaction. Test compounds were prepared as 20 mM stock solutions in DMSO (Molecular Biology grade, Sigma-Aldrich Co., St. Louis, MO) and stored at -70 'C. Serial dilutions of test compounds were prepared in assay buffer immediately prior to testing. DMSO was determined in a separate trial to have no significant effect on the 10 activity of this assay at concentrations up to 5%; the final DMSO concentration in the wells was no more than 2% and therefore DMSO effects were safely neglected. Assays were performed in white polystyrene 96-well half-area assay plates (Coming, Corning, NY) and measured on a Wallace 1420 fluorescent plate reader (Wallac Oy, Turku, Finland) with an excitation wavelength of 355 nm, an emission wavelength of 460 nm, and a 1 sec signal 15 averaging time. The following table shows the percent inhibition of HDAC produced by some of the examples of the present invention at a concentration of 100 pM. Table 1. Inhibition of histone deacetylase at 100 pM concentration. 20 Example Number % Inhibition of HDAC @ 100 pM 3 97.9 4 99.1 5 99.7 9 99.9 10 99.5 11 99.8 12 99.9 13 99.9 14 99.9 15 99.8 16 98.9 18 98.5 19 97.6 20 99.6 21 98.5 181 WO 2006/088949 PCT/US2006/005312 22 98.2 23 99.3 24 99 25 98 26 98.9 27 98.3 28 95.2 29 99.8 30 98.1 31 100 32 96.6 34 99.1 35 99.9 36 99.8 37 99.9 38 99.8 39 98.8 40 99.8 41 99.6 42 100 43 100 44 99.9 45 99.9 46 99.8 47 100 48 99.7 49 99.9 50 99.7 51 99.8 52 99.7 53 100 54 99.5 55 99.3 56 99.8 57 99.9 58 97.7 59 97.3 60 100 61 99.9 62 99.9 182 WO 2006/088949 PCT/US2006/005312 63 99.8 64 99.9 65 99.8 67 99.9 68 99.9 70 99.9 71 99.9 72 99.8 73 99.6 74 99.1 75 99.9 76 100 77 100 78 99.9 79 100 82 99.9 83 99.9 84 99.9 86 100 87 100 88 100 90 99.8 91 100 92 99.9 93 100 94 99.7 95 100 96 100 97 100 99 99.9 100 100 101 100 103 99.9 104 100 105 99.9 106 99.9 107 99.9 183 WO 2006/088949 PCT/US2006/005312 108 100 109 100 110 100 111 99.9 112 99.9 113 100 114 99.9 115 99.9 116 100 117 99.8 118 99.7 119 99.3 120 99.6 121 99.6 122 100 123 100 In some assays recombinant HDAC8 (Biomol) was used as the source of the enzyme activity; here the final substrate concentration was 250 pM, the final concentration of HDAC8 was 0.02 Units/pL and the reaction was allowed to proceed at 37 'C for 1 h before 5 stopping. For all curves, IC 50 values were calculated with the GraFit curve-fitting program (Erithacus, Horley, Surrey, UK). Example B Whole Cell Cytotoxicity Assay:Sulforhodamine B The following procedure can be found on the Developmental Therapeutics Program 10 NCI/NIH web site at http://dtp.nci.nih.gov/brancehes/btb/ivclsp.html. 1. Human tumor cell lines of HT29, A549 and MCF7 are grown in DMEM containing 10% fetal bovine serum and 2 mM L-glutamine. Cells are plated in a 96 well plate at a density of 5000 cells per well in 100 piL of growth medium and incubated at 37 0 C, 5% C0 2 , for 24 hours prior to the addition of experimental compounds. 15 2. Experimental drugs are solubilized in DMSO for a final concentration of 20 mM immediately prior to use. Drugs are further diluted in growth media for a total of 184 WO 2006/088949 PCT/US2006/005312 nine drug concentrations and a growth control. At the 24 hour time point, one plate of cells is fixed in situ with TCA as a measurement of the cell population at time zero, or the time of drug addition. 5 3. The plates are further incubated with drug for an additional 48 hours. 4. The cells are fixed in situ by gently aspirating off the culture media and then adding 50 ptL of ice cold 10% TCA per well and incubated at 4 0 C for 60 minutes. The plates are washed with tap water five times and allowed to air dry for 5 minute. 10 5. 50 ptL of a 0.4% (w/v) Sulforhodamine B solution in 1% (v/v) acetic acid is added per well and incubated for 30 minutes at room temperature. 6. Following staining, plates are washed five times with 1% acetic acid to remove any 15 unbound dye and then allowed to air dry for 5 minutes. 7. Stain is solubilized with 100 ptL of 10 mM Tris pH 10.5 per well and placed on an orbital rotator for 5 minutes. 20 8. Absorbance is read at 570 nm. The following table shows the percent inhibition of MCF7 cell growth produced by some of the examples of the present invention at a concentration of 100 EM. 25 Table 2. Inhibition of HCTI 16 cell growth by examples at a 100 pIM concentration. Example Number % Inhibition of HCT116 cell growth @ 100 pM 3 98.3 4 98.5 5 99.9 9 99.3 10 98.4 11 99 185 WO 2006/088949 PCT/US2006/005312 12 98.7 13 99.1 14 98.1 15 99 16 99 18 98.5 19 97.2 20 98.2 21 94 22 98.2 23 98.1 24 98.9 25 94 26 99 27 98.9 28 98.7 29 98 30 96.8 31 99.5 32 94.6 34 99.3 35 97.3 36 98.3 37 97.1 38 99.5 39 99.4 40 98.3 41 98.7 42 88.0 43 98.7 44 97.6 45 98.9 46 98.6 47 98.1 48 97.8 49 99.3 50 89.1 51 98.7 52 98.9 53 96.4 54 98.9 55 99.1 56 99.0 57 98.2 58 96.3 186 WO 2006/088949 PCT/US2006/005312 59 97.5 60 99.0 61 98.2 62 96.2 63 98.9 64 98.7 65 98.4 67 98.2 68 98.9 70 97.9 71 98.6 72 95.6 73 99.4 74 93.6 75 98.4 76 98.9 77 98.1 78 85.1 79 95.7 82 97.0 83 94.7 84 93.2 86 99.1 87 83.1 88 98.4 90 93.1 91 98.6 92 98.9 93 98.0 94 98.5 95 98.2 96 98.7 97 98.1 99 93.8 100 96.6 101 97.9 103 98.9 104 92.0 105 97.0 106 98.3 107 92.7 108 99.1 109 91.7 110 98.7 111 97.7 187 WO 2006/088949 PCT/US2006/005312 112 98.7 113 98.7 114 99.3 115 98.8 116 98.6 117 98.6 118 98.9 119 98.6 120 97.6 121 97.5 122 44.4 123 97 188

Claims

1. A compound of formula I: W4 O A N-W-2Ar2--G W NHOH 5 wherein: is Ar which is selected from the group consisting of substituted 1,2-fused aryl, 1,2 fused heteroaryl, substituted 1,2-fused heteroaryl, 1,2-fused heterocyclic, and substituted 1,2-fused heterocyclic; 10 Ar' is fused to the ring containing W1 and W; W and W1 are independently [-C(R')(R 2 )-]m; W 2 is a bond or [-C(R')(R2 R' and R 2 are independently selected from the group consisting of hydrogen, alkyl, substituted alkyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, heterocyclic, and 15 substituted heterocyclic; each m is independently 1 or 2; pis 1,2,3 or4; Ar2 is selected from the group consisting of arylene, substituted arylene, heteroarylene, and substituted heteroarylene; and 20 G is selected from the group consisting of a bond, alkylene, substituted alkylene, alkenylene, substituted alkenylene, alkynylene, and substituted alkynylene; or a tautomer, stereoisomer, prodrug, or pharmaceutically acceptable salt thereof; provided that when Ar2 is arylene or substituted arylene, then G is not alkenylene or substituted alkenylene. 25

2. The compound according to Claim 1, wherein Ar' is 1,2-fused heteroaryl, substituted 1,2-fused heteroaryl, 1,2-fused heterocyclic, or substituted 1,2-fused heterocyclic. 189 WO 2006/088949 PCT/US2006/005312

3. The compound according to Claim 2, wherein Ar and the ring to which it is fused together form a heterocyclic group selected from the group consisting of: optionally substituted 1,3,4,9-tetrahydro-2H-b-carbolin-2-yl, optionally substituted 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl, 5 optionally substituted 1,4,5,6-tetrahydroazepino[4,5-b]indol-3(2H)-yl, optionally substituted 1,3,4,4a,9,9a-hexahydro-2H-b-carbolin-2-yl, optionally substituted 1,1a,3,4,4a,5-hexahydro-2H-pyrido[4,3-b]indol2-yl, optionally substituted 10-oxo-3,4,5,10-tetrahydrobenzo[b]-1,6-naphthyridin-2(1H) yl, 10 optionally substituted 3,4-dihydro[1]benzothieno[2,3-c]pyridin-2(1H)-yl and optionally substituted 3,4-dihydro[1]benzofuro[2,3-c]pyridin-2(1H)-yl.

4. The compound according to Claim 3, wherein Ar 2 is arylene or substituted arylene.

5. The compound according to Claim 4, wherein said compound is 0 H ' NHOH N N 15 [N-hydroxy-4-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)benzamide].

6. The compound according to Claim 2, wherein Ar 2 is heteroarylene or substituted heteroarylene.

7. The compound according to Claim 6, wherein said heteroarylene or substituted heteroarylene group, together with -G-C(O)NHOH, is selected from the group consisting of 20 5-[-G-C(O)NHOH]-pyrimid-2-ylene, 5-[-G-C(O)NHOH]-thiazol-2-ylene, 5-[-G C(O)NHOH]-pyrid-2-ylene and the N-oxide thereof, and 5-[-G-C(O)NHOH]-thien-2-ylene.

8. The compound according to claim 7, wherein said compound is 190 WO 2006/088949 PCT/US2006/005312 0 N NHOH H N N N [N-hydroxy-3-[2-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidin-5 yl]propanamide].

9. The compound according to Claim 7, wherein G is optionally substituted alkenylene. 5

10. The compound according to Claim 9, wherein Ar' and the ring to which it is fused together form a heterocyclic group selected from the group consisting of: optionally substituted 1,3,4,9-tetrahydro-2H-b-carbolin-2-yl, optionally substituted 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl, optionally substituted 1,4,5,6-tetrahydroazepino[4,5-b]indol-3(2H)-yl, 10 optionally substituted 1,3,4,4a,9,9a-hexahydro-2H-b-carbolin-2-yl, optionally substituted 1,1a,3,4,4a,5-hexahydro-2H-pyrido[4,3-b]indol-2-yl, optionally substituted 10-oxo-3,4,5,10-tetrahydrobenzo[b]-1,6-naphthyridin-2(1H) yl, optionally substituted 3,4-dihydro[1]benzothieno[2,3-c]pyridin-2(1H)-yl and 15 optionally substituted 3,4-dihydro[1]benzofuro[2,3-c]pyridin-2(1H)-yl.

11. The compound according to claim 10, wherein said compound is: 0 N NHOH H N N [(2E)-N-hydroxy-3-[2-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidin-5-yl]acrylamide] or 191 WO 2006/088949 PCT/US2006/005312 NH N NHOH 0 [(2E)-N-hydroxy-3-[5-(1,3,4,9-tetrahydro-2H-b-carbolin-2-ylmethyl)thien-2-yl]acrylamide].

12. The compound according to Claim 7, wherein G is a bond.

13. The compound according to Claim 12, wherein Ar' and the ring to which it is fused 5 together form a heterocyclic group selected from the group consisting of: optionally substituted 1,3,4,9-tetrahydro-2H-b-carbolin-2-yl, optionally substituted 1 ,3,4,5-tetraiaydro-2H-pyrido [4,3 -b]indol-2-yl, optionally substituted 1,4,5,6-tetrahydroazepino[4,5-b]indol-3(2H)-yl, optionally substituted 1,3,4,4a,9,9a-hexahydro-2H-b-carbolin-2-yl, 10 optionally substituted 1,1a,3,4,4a,5-hexahydro-2H-pyrido[4,3-b]indol-2-yl, optionally substituted 10-oxo-3,4,5,10-tetrahydrobenzo[b]-1,6-naphthyridin-2(1H) yl, optionally substituted 3,4-dihydro[1]benzothieno[2,3-c]pyridin-2(1H)-yl and optionally substituted 3,4-dihydro[1]benzofuro[2,3-c]pyridin-2(1H)-yl. 15

14. The compound according to Claim 13, wherein W 2 is a bond.

15. The compound according to Claim 14, wherein said compound is N H N SO NHOH [N-hydroxy-2-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)-1,3-thiazole-5-carboxamide] or 0 O H i-H N N N 20 [N-hydroxy-6-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)nicotinamide]. 192 WO 2006/088949 PCT/US2006/005312

16. The compound according to Claim 12, wherein said heteroarylene group, together with -G-C(O)NHOH, is 5-[-G-C(O)NHOH]-pyrimid-2-ylene.

17. The compound according to Claim 16, wherein Ar' and the ring to which it is fused together form an optionally substituted 1,3,4,9-tetrahydro-2H-b-carbolin-2-yl or optionally 5 substituted 1,3,4,4a,9,9a-hexahydro-2H-b-carbolin-2-yl substituent.

18. The compound according to Claim 17, wherein W 2 is a bond.

19. The compound according to Claim 18, wherein said compound is: 0 N NHOH H N CN N [N-hydroxy-2-(1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5-carboxamide], 0 N NHOH H N N N NHO 10 MeO [N-hydroxy-2-(6-methoxy-1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5 carboxamide], 0 N~ NHOH N N [N-hydroxy-2-(8-methyl-1,3,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5-carboxamide], 193 WO 2006/088949 PCT/US2006/005312 0 NN ~ NHOH HI N NIN [N-hydroxy-2-(7-fluoro- 1,3 ,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5-carboxamide], 0 N'~- NHOH IN N N) F [N-hydroxy-2-(6-fluoro- 1,3 ,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5-carboxamide], 0 N ~ NHOH HI N NI 5 0o [N-hydroxy-2-(6-benzyloxy- 1,3 ,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5 carboxamide], 0 N - NHOH HI N NIN. 10 [N-hydroxy-2-(7-methyl- 1,3 ,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5-carboxamide], 0 N~- NHOH H N N 'N 194 WO 2006/088949 PCT/US2006/005312 [N-hydroxy-2-(6-methyl- 1,3 ,4,9-tetrahiydro-2H-b-carbolin-2-yl)pyrimidine-5-carboxamide], 0 N~ NHOH 0 N [N-hydroxy-2-1{6-[3 -(morpholin-4-ylmethyl)phenyl]- 1,3 ,4,9-tetrahydro-2H-b-carbolin-2 yllpyrimidine-5-carboxamide], 0 N~- NHOH NoN N 5 [N-hydroxy-2- {6- [3-((4-methylpiperazin- 1-yl)methyl)phenyl] -1,3 ,4,9-tetrahydro- 2H-b carbolin-2-yl}pyrimidine-5-carboxamide], 0 NN ~ NHOH H N NA)'lN -NN 10 [N-hydroxy-2- { 6- [4-((4-methylpiperazin- 1-yl)methyl)phenyl]- 1,3 ,4,9-tetrahydro-2H-b carbolin-2-yl}pyrimidine-5-carboxamide], 195 WO 2006/088949 PCT/US2006/005312 0 N- NHOH N N N N [N-hydroxy-2- {6-[2-((4-methylpiperazin- 1-yl)methyl)phenyl] -1,3 ,4,9-tetrahydro- 2H-b carbolin-2-yllpyrimidine-5-carboxamide], N N I- N H 5 [N-hydroxy-2- {9-[12-pyrrolidin- 1 -ylethyl] -1,3 ,4,9-tetrahydro-2H-b- carbolin-2 yllpyrimidine-5-carboxamide], ON 0 N -- NHOH NN'N [N-hydroxy-2-[9-(2-piperidin- 1-ylethyl)- 1,3 ,4,9-tetrahydro-2H-b-carbolin-2-yl]pyrimidine 5-carboxamide], 0 N 0 N N N HO 10 IN [N-hydroxy-2-[9-(2-morpholin-4-ylethyl)- 1,3 ,4,9-tetrahydro-2H-b- carbolin-2 196 WO 2006/088949 PCT/US2006/005312 yl]pyrimidine-5.-carboxamnide], N~la NHOH [N-hydroxy-2-[9-(phenylsulfonyl)- 1,3 ,4,9-tetrahydro-2H-b- carbolin-2-yl]pyrimidine-5 carboxamide], 0 NN~~NHOH N NN [N-hydroxy-2-(9-methyl- 1,3 ,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5-carboxamide], N ~ NHOH N NIN" [N-hydroxy-2-(9-benzyl- 1,3 ,4,9-tetrahydro-2H-b-carbolin-2-yl)pyrimidine-5-carboxamide], 0 0 N ~ NHOH NN<N 10 [2-(9-acetyl- 1,3 ,4,9-tetrahydro-2H-b-carbolin-2-yl)-N-hydroxypyrimidine-5-carboxamide], 0 (2>- N' NHOH [N-hydroxy-2- {9-12-pyrrolidin- 1-ylmethyl]- 1,3 ,4,9-tetrahydro-2H-b-carbolin-2 yl}pyrimidine-5-carboxamide], 197 WO 2006/088949 PCT/US2006/005312 HO 0 N -' NHOH N [N-hydroxy-2-[9-(2-hydroxyethyl)-1,3,4,9-tetrahydro-2H-b-carbolin-2-yl]pyrimidine-5 carboxamide], N/ 0 N NHOH IN N N 5 [N-hydroxy-2-[9-(1-methylpiperidin-3-yl)-1,3,4,9-tetrahydro-2H-b-carbolin-2 yl]pyrimidine-5-carboxamide] or 0 NHOH H N N N [N-hydroxy-2-(6-methyl-1,3,4,4a,9,9a-hexahydro-2H-b-carbolin-2-yl)pyrimidine-5 carboxamide]. 10

20. The compound according to Claim 16, wherein Ar' and the ring to which it is fused together form a heterocyclic group selected from the group consisting of optionally substituted 1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl and optionally substituted 1,1a,3,4,4a,5-hexahydro-2H-pyrido[4,3-b]indol-2-yl substituent.

21. The compound according to Claim 20, wherein W 2 is a bond. 198 WO 2006/088949 PCT/US2006/005312

22. The compound according to Claim 21, wherein said compound is: 0 N~ NHOH N N NO H [N-hydroxy-2-(1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5-carboxamide], 0 N NHOH NIN N H 5 [N-hydroxy-2-(8-methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5 carboxamide], 0 CI N NHOH N N H [2-(8-chloro-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)-N-hydroxypyrimidine-5 carboxamide], 0 Br N NHOH N N N 10 H [2-(8-bromo-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)-N-hydroxypyrimidine-5 carboxamide], 0 F 3 CO N NHOH N N H [N-hydroxy-2-[8-(trifluoromethoxy)-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 199 WO 2006/088949 PCT/US2006/005312 yl]pyrimidine-5-carboxamide], 0 N NHOH \ /N N N H [N-hydroxy-2-(8-phenyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5 carboxamide], 0 F N NHOH I NIN N 5 H [N-hydroxy-2-(8-fluoro-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5 carboxamide], 0 MeO N NHOH - N N N H [N-hydroxy-2-(8-methoxy-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5 10 carboxamide], 0 N NHOH FN N F- 3 IN N H [N-hydroxy-2-(7-fluoro-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5 carboxamide], 0 N NHOH N H 200 WO 2006/088949 PCT/US2006/005312 [N-hydroxy-2-(7-methyl- 1,3 ,4,5-tetrahydro-2H-pyrido[4,3 -b]indol-2-yl)pyrimidine-5 carboxamide], 0 N~-~NHOH N H [N-hydroxy-2-(7,8-dimethyl- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 -b]indol-2-yl)pyrimidine-5 5 carboxamide], 0 N~ NHOH CN I N)' N H [N-hydroxy-2-(7-chloro-8-methyl- 1,3 ,4,5-tetraliydro-2H-pyrido [4,3 -b]indol-2 yl)pyrimidine-5-carboxamnide], 0 N -- -" NHOH BrQ IN N] H 10 [N-hydroxy-2-(7-bromo- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 -b]indol-2-yl)pyrimidine-5 carboxamide], 0 F 3 C N NHOH -I NN) N H [N-hydroxy-2-(8-trifluoromethyl- 1,3,4,5-tetrahydro-2H-pyrido [4,3 -b]indol-2-yl)pyrimidine 5-carboxamide], 201 WO 2006/088949 PCT/US2006/005312 0 Nc-' NHOH - NIN N H [N-hydroxy-2-(9-chloro-8-methyl- 1,3,4,5 -tetrahydro-2H-pyrido [4,3 -b]indol-2 yl)pyrimidine-5-carboxamnide], 0 0 2 N N NHOH I NN N H 5 [N-hydroxy-2-(8-nitro- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 -b]indol-2-yl)pyrimidine- 5 carboxamide], HN N- NHOH N N N H [N-hydroxy-2-(8-(N-acetamido)- 1,3 ,4,5-tetrahydro-2H-pyrido[4,3 -b]indol-2-yl)pyrimidine 5-carboxamide], S-L0 0 HN N NHOH I NIN N 10 H [N-hydroxy-2-(8-(N-methylsulfonamido)- 1,3 ,4,5-tetrahydro-2H-pyrido[4,3 -b]indol-2 yl)pyrimidine-5-carboxamnide], 0 H 2 N N - NHOH - NN. N H [N-hydroxy-2-(8-amino- 1,3 ,4,5-tetrahydro-2H-pyrido[4,3 -b]indol-2-yl)pyrimidine-5 202 WO 2006/088949 PCT/US2006/005312 carboxamide], 0 N NHOH N N N H [N-hydroxy-2-{8-isopropyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5 carboxamide], 0 N NHOH N N 5 H [N-hydroxy-2-{8-tert-butyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5 carboxamide], 0 N NHOH - N N N' H [N-hydroxy-2-{7-phenyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5 10 carboxamide], 0 H 3 C N NHOH MNN [N-hydroxy-2-{5-acetyl-8-methyl-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl}pyrimidine-5-carboxamide], 0 N NHOH N IN)A N H 203 WO 2006/088949 PCT/US2006/005312 [N-hydroxy-2-(8-methyl-1,3,4,4a,5,9b-hexahydro-2H-pyrido[4,3-b]indol-2-yl)pyrimidine-5 carboxamide] or O -N N N NHOH NN N H [N-hydroxy-2-{ 8-[(4-methylpiperazin-1-yl)methyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 5 b]indol-2-yl}pyrimidine-5-carboxamide].

23. The compound according to Claim 20, wherein said compound is: 0 N NHOH N H [N-hydroxy-2-{8-[3-(pyrrolidin-1-ylmethyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl}pyrimidine-5-carboxamide], N 0 N - NHOH N N N 10 H [N-hydroxy-2-{8-(pyridin-3-yl)-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2-yl}pyrimidine 5-carboxamide], 0 N NHOH N N' N H [N-hydroxy-2-{ 8-[4-(pyrrolidin-1-ylmethyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 204 WO 2006/088949 PCT/US2006/005312 b]indol-2-yl}pyrimidine-5-carboxamide], N/ N . NHOH N H [N-hydroxy-2- { 8-(pyrimidin-5-yl)- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 -b]indol-2 yl}pyrimidine-5-carboxamide], N0 N~- NHOH N 5 H [N-hydroxy-2- {8-[3 -(morpholin-4-ylmethyl)phenyl]- 1,3,4,5 -tetrahydro-2H-pyrido [4,3 b]indol-2-yl}pyrimidine-5-carboxamnide], N0 N _'- NHOH NIN N H [N-hydroxy-2- {8[3 -(piperidin- 1-ylmethyl)phenyl]- 1,3 ,4,5-tetrahydro-2H- pyrido [4,3 10 b]indol-2-yllpyrimidine-5-carboxamnide], -NJ 0 I' NHOH NN H [N-hydroxy-2- {8-[3 -(N, N-dimethylaminomethyl)phenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl}pyrimidine-5-carboxamide], 205 WO 2006/088949 PCT/US2006/005312 N0 N~ NNHOH NN OH N H [N-hydroxy-2-{8-[3-((4-hydroxymethylpiperidin-1-yl)methyl)phenyl]-1,3,4,5-tetrahydro 2H-pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide], N0 NN NHOH N H 5 [N-hydroxy-2-{ 8-[3-(thiomorpholin-4-ylmethyl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl}pyrimidine-5-carboxamide], N0 HO N N NHOH OH - N N OHN ~ I N H [N-hydroxy-2-{8-[3-(N,N-di(2-hydroxyethyl)aminomethyl)phenyl]-1,3,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide], N 0 N NHOH NJ ) f -- N N 10 H [N-hydroxy-2-{8-[3-((4-methylpiperazin-1-yl)nethyl)phenyl]-1,3,4,5-tetrahydro- 2H pyrido[4,3-b]indol-2-yl}pyrimidine-5-carboxamide], 0 H N NHOH N HNN H 206 WO 2006/088949 PCT/US2006/005312 [N-hydroxy-2-1{8-[3 -(piperazin- 1-ylmethyl)phenyl]- 1,3,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl}pyrimidine-5-carboxamide], N0 N~ NHOH IN NIN' H [N-hydroxy-2- {8- [3-((4-(methylsulfonyl)piperazin- 1-yl)methyl)phenyl]- 1,3,4,5 5 tetrahydro-2H-pyrido[4,3 -b]indol-2-yllpyrimidine-5-carboxamide], N0 N NHOH Nm H [N-hydroxy-2- {8-[3 -((4-acetylpiperazin- 1-yl)methyl)phenyl] -1,3 ,4,5-tetrahydro- 2H pyrido[4,3 -b]indol-2-yl}pyrimidine-5-carboxamide], o N 0 N'~ NHOH N H 10 [N-hydroxy-2- {8-[4-(morpholin-4-ylmethyl)phenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yl}pyrimidine-5-carboxamide], 0 N~- NHOH NM H [N-hydroxy-2- { 8-[4-(thiomorpholin-4-ylmethyl)phenyl]- 1,3,4,5-tetrahydro-2H-pyrido [4,3 b]indol-2-yllpyrimidine-5-carboxamide], 207 WO 2006/088949 PCT/US2006/005312 N NJ 0 N - NHOH - NIN H [N-hydroxy-2- {8-[4-((4-methylpiperazin- 1-yl)methyl)phenyl]- 1,3 ,4,5-tetrahydro- 2H pyrido [4,3 -b]indol-2-yl}pyrimidine-5-carboxamnide], OH 0 N - NHOH - NN N H 5 [N-hydroxy-2- {8- [4-(hydroxymethyl)phenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 - b]indol 2-yl}pyrimidine-5-carboxamide], N 0/ N 1- NHOH N H [N-hydroxy-2- {8 8-[5 -((4 -methylpiperazin- 1 -yl)methyl) -2-furyl] - 1, 3,4,5 -tetrahydro-2H pyrido[4,3 -b]indol-2-yllpyrimidine-5-carboxamide], N 0 o") NHOH N 10 H [N-hydroxy-2- {8-[5-(morpholin-4-yl)methyl-2-furyl]- 1,3,4,5-tetrahydro-2H-pyrido[4,3 blindol-2-yl}pyrimidine-5-carboxamide], 208 WO 2006/088949 PCT/US2006/005312 N 0 /'NN ~ NHOH N H [N-hydroxy-2- {8-[5 -((4-methylpiperazin- 1-yl)methyl)-thien-2-yl] -1,3,4,5- tetrahydro-2H pyrido [4,3 -b]indol-2-yllpyrimidine-5-carboxamide], HOH 2 C \/0 N~ NHOH N H 5 [N-hydroxy-2- {8-[3 -(hydroxymethyl)phenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 - b]indol 2-yl}pyrimidine-5-carboxamide], 0 00 N - NHOH NIN' N H [N-hydroxy-2-1{8-[4-methoxyphenyl] -1,3 ,4,5-tetrahydro-2H-pyrido[4,3 -b]indol-2 yl}pyrimidine-5-carboxamide], 0 N - NHOH - NIN N 10 H [N-hydroxy-2-1{8-[4-methylphenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido[4,3 -b]indol-2 yl}pyrimidine-5-carboxamide], 209 WO 2006/088949 PCT/US2006/005312 N~N NHOH - NIN N H [N-hydroxy-2- {8-[4-(N-acetamido)phenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yllpyrimidine-5-carboxamide], 0 N- NHOH 1 NN N H 5 [N-hydroxy-2-1{8-(fur-2-yl)-l ,3 ,4,5-tetrahydro-2H-pyrido [4,3 -b]indol-2-yllpyrimidine-5 carboxamide], 0 ON N~' NHOH H [N-hydroxy-2- {8-[3 -(pyrrolidin- 1-ylcarbonyl)phenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido[4,3 b]indol-2-yllpyrimidine-5-carboxarnlide], 0O N~ NHOH N 10 H [N-hydroxy-2- {7-[3 -(pyrrolidin- 1-ylmethyl)phenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 b]indol-2-yllpyrimidine-5-carboxamnide], 210 WO 2006/088949 PCT/US2006/005312 H 2 N- 0 N~ NHOH N H [N-hydroxy-2- {8-[3 -aminophenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 -b]indol-2 yllpyrimidine-5-carboxamide], F 0 N~ NHOH NIN N H 5 [N-hydroxy-2- {8-[3 -fluorophenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 -b]indol-2 yllpyrimidine-5-carboxamide], F 0 N - NHOH N H [N-hydroxy-2- { 8- [4-fluorophenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido[4,3 -b]indol-2 yllpyrimidine-5-carboxamide], 0 c~aDN 0 NHOH N 10 H [N-hydroxy-2- {8- [fur-3 -yl]-l ,3,4,5-tetrahydro-2H-pyrido [4,3 -b]indol-2-yllpyrimidine-5 carboxamide], 211 WO 2006/088949 PCT/US2006/005312 0 HN/ N . NHOH N H [N-hydroxy-2- {8-{1H-pyrrol-2-yll- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 -b]indol-2 yllpyrimidine-5-carboxamide], N NHOH N H 5 [N-hydroxy-2- {8-[3 -(methylthio)phenyl]- 1,3 ,4,5-tetrahydro-211-pyrido [4,3 b]indol-2-yl~pyrimidine-5-carboxamide], -N/ 0 N ~ NHOH NIN H [N-hydroxy-2- {8-[4-dimethylaminophenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 bllindol-2-yl}pyrimidine-5-carboxamide], 0 0 N N~NHOH NN N 10 H [N-hydroxy-2- { 8-[3 -(methylsulfinyl)phenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2 yl}pyrimidine-5-carboxamide], 212 WO 2006/088949 PCT/US2006/005312 0 0 N'NH NHOH N'N N H [N-hydroxy-2- {8-[3 -(N-acetamidomethyl)phenyl]- 1,3 ,4,5-tetrahydro-2H- pyrido [4,3 b]indol-2-yl}pyrimidine-5-carboxamide], 0\ 0 S-NH N ~ NHOH NIN N H 5 [N-hydroxy-2-1{8-[3 -(N-methylsulfonamnidomethyl)phenyl]- 1,3 ,4,5-tetrahydro-2H pyrido[4,3-b]indol-2-yllpyrimidine-5-carboxamide], HN 0 N NHOH NIN) N H [N-hydroxy-2- {8- [3 -(N-acetamido)phenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 -b]indol-2 yl}pyrimidine-5-carboxamide], HN 0 _/' N NHOH N 10 H [N-hydroxy-2- {8- [3 -(N- methylsulfonarnido)phenyl]- 1,3 ,4,5-tetrahydro-2H-pyrido [4,3 b]indol-2-yl}pyrimidine-5-carboxainide], 213 WO 2006/088949 PCT/US2006/005312 NN H N- NHOH NN N H [N-hydroxy-2-{8-[3-(1H-tetrazol-5-yl)phenyl]-1,3,4,5-tetrahydro-2H-pyrido[4,3- b]indol 2-yl}pyrimidine-5-carboxamide] or 0 N NHOH NN N H 5 [N-hydroxy-2-{8-[thien-2-yl]-1,3,4,5-tetrahydro-2H-pyrido[4,3-b]indol-2- yl}pyrimidine-5 carboxamide].

24. The compound according to Claim 16, wherein Ar' and the ring to which it is fused together form an optionally substituted 1,4,5,6-tetrahydroazepino[4,5-b]indol-3(2H)-yl group. 10

25. The compound according to Claim 24, wherein W 2 is a bond.

26. The compound according to claim 25, wherein said compound is: 0 N NHOH N H [N-hydroxy-2-(1,4,5,6-tetrahydroazepino[4,5-b]indol-3(2H)-1-yl)pyrimidine-5 carboxamide], 214 WO 2006/088949 PCT/US2006/005312 0 N NHOH O N N K N H [N-hydroxy-2-(9-methoxy-1,4,5,6-tetrahydroazepino[4,5-b]indol-3(2H)-i-yl)pyrimidine-5 carboxamide], 0 N NHOH F N N aN H 5 [N-hydroxy-2-(9-fluoro-1,4,5,6-tetrahydroazepino[4,5-b]indol-3(2H)-1-yl)pyrimidine-5 carboxamide], 0 N ~ NHOH N N N [N-hydroxy-2-(9-[3-(morpholin-4-ylmethy)phenyl]-1,4,5,6-tetrahydroazepino[4,5-b]indol 3(211)-1-yl)pyrimidine-5-carboxamide] or 0 N ~ NHOH N N N 100 H 101 [N-hydroxy-2-(9-[3-((4-methylpiperazin-1-yl)methy)phenyl]-1,4,5,6-tetrahydroazepino[4,5 b]indol-3(2H)-1-yl)pyrimidine-5-carboxamide]. 215 WO 2006/088949 PCT/US2006/005312

27. The compound according to Claim 15, wherein said heterocyclic group is selected from the group consisting of: optionally substituted 10-oxo-3,4,5,10-tetrahydrobenzo[b]-1,6-naphthyridin-2(1H) yl, 5 optionally substituted 3,4-dihydro [1 ]benzothieno [2,3 -c]pyridin-2(1 H)-yl, and optionally substituted 3,4-dihydro[1]benzofuro[2,3-c]pyridin-2(1H)-yl.

28. The compound according to Claim 27, wherein W 2 is a bond.

29. The compound according to Claim 28, wherein said compound is: 0 N~ NHOH S N N 10 [2-(3,4-dihydro[1]benzothieno[2,3-c]pyridin-2(1H)-yl)-N-hydroxypyrimidine-5 carboxamide], 0 N NHOH N N [2-(3,4-dihydro[1]benzofuro[2,3-c]pyridin-2(1H)-yl)-N-hydroxypyrimidine-5-carboxamide] or 0 0 N- 0 Nk NHOH 15 H [N-hydroxy-2-(10-oxo-3,4,5,10-tetrahydrobenzo[b]-1,6-naphthyridin-2(1H)-yl)pyrimidine 5-carboxamide].

30. The compound according to Claim 1, wherein Ar' is 1,2-fused substituted aryl. 216 WO 2006/088949 PCT/US2006/005312

31. The compound according to Claim 30, wherein Ar 2 is heteroarylene or substituted heteroarylene.

32. The compound according to Claim 31, wherein said heteroarylene or substituted heteroarylene group, together with -G-C(O)NHOH, is selected from the group consisting of 5 5-[-G-C(O)NHOH]-pyrimid-2-ylene, 5-[-G-C(O)NHOH]-thiazol-2-ylene, 5-[-G C(O)NHOH]-pyrid-2-ylene and the N-oxide thereof and 5-[-G-C(O)NHOH]-thien-2-ylene.

33. The compound according to Claim 32, wherein G is optionally substituted alkenylene.

34. The compound according to Claim 33, wherein G is a bond. 10

35. The compound according to Claim 34, wherein said heteroarylene group, together with -G-C(O)NHOH, is selected from the group consisting of 5-[-G-C(O)NHOH]-pyrimid 2-ylene and 5-[-G-C(O)NHOH]-pyrid-2-ylene and the N-oxide thereof.

36. The compound according to Claim 35, wherein W 2 is a bond.

37. The compound according to Claim 36, wherein said compound is: N0 N, 15 HN-OH [6-(6,7-dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl)-N-hydroxynicotinamide], 0 N NHOH - IN N [2-(6,7-dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl)-N-hydroxypyrimidine-5 20 carboxamide], or 217 WO 2006/088949 PCT/US2006/005312 /N: NHOH H 3 CO [2-(7-methoxy-3,4,dihydroisoquinolin-2(1H)-yl)-N-hydroxypyrimidine-5- carboxamide].

38. A pharmaceutical composition comprising an effective amount of one or more compounds according to Claim 1-37 and a pharmaceutically inert carrier. 5

39. A pharmaceutical composition comprising an effective amount of one or more compounds according to Claim 1-37 and an effective amount of at least one anti-cancer agent, and a pharmaceutically inert carrier.

40. Use of a compound of any one of Claims 1-37 for the manufacture of a medicament for inhibiting a proliferative disorder in a mammalian patient. 10

41. The use according to Claim 40, wherein said proliferative disorder is cancer. 218