AU2005202649A1 - Benzodiazepine derivatives for imaging thrombi - Google Patents

Description

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Our Ref: 12563791 P/00/011 Regulation 3:2

AUSTRALIA

Patents Act 1990

ORIGINAL

COMPLETE SPECIFICATION STANDARD PATENT Applicant(s): Diatide, Inc.

9 Delta Drive Londonderry New Hampshire 03053 United States of America Genentech, Inc.

One DNA Way South San Francisco California 94080 United States of America Address for Service: Invention Title: DAVIES COLLISON CAVE Patent Trade Mark Attorneys Level 10, 10 Barrack Street Sydney, New South Wales, Australia, 2000 Benzodiazepine derivatives for imaging thrombi The following statement is a full description of this invention, including the best method of performing it known to me:- 5951

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WO 00/61195 PCT/US00/10093 BENZODIAZEPINE DERIVATIVES FOR IMAGING THROMBI The present invention relates to the field of diagnostic imaging of thrombosis.

More particularly, the invention relates to pharmaceutical compositions for imaging thrombi.

BACKGROUND OF THE INVENTION Thrombi are blood clots which form within the cardiovascular system.

Formation of thrombi, thrombosis, may cause local obstruction of blood vessels, for example in veins, arteries, or capillaries. Venous thrombi usually form in lower limbs and may produce acute symptoms by causing inflammation of the vessel wall or obstruction of the vein. Pieces of venous thrombi may also circulate through the cardiovascular system to form a plug, or embolus, at a distant site such as the lung.

Arterial thrombi are commonly associated with vascular disease such as atherosclerosis and may produce tissue ischemia (local anemia) by obstructing blood flow or by embolizing into capillaries. Thrombi may also form in the heart, for example, on inflamed or damaged valves, on tissue adjacent to myocardial infarcts, within injured chambers, or on prosthetic valves.

While all thrombi contain the protein fibrin and blood cells, the proportions of particular blood cells present and of fibrin/blood cell may differ, for example, because of the blood flow at the site of thrombus formation and thrombus age. Arterial thrombi which form at sites of high blood flow contain platelet aggregates bound together by thin fibrin strands. Venous thrombi form in areas of stagnant blood flow and contain red blood cells with interspersed fibrin and fewer platelets. Thrombi which form under conditions of slow to moderate flow contain a mixture of red cells, platelets, and fibrin.

Leukocytes, the white blood cells, migrate to and become incorporated into thrombi as they age. In addition, aggregated platelets in aging thrombi lyse and are replaced by fibrin.

Accurate detection of the various kinds of thrombi is necessary to choose, to optimize and to monitor treatment, which may differ by virtue of the location and nature WO 00/61195 PCT/US00/10093 of the thrombus. Recently, ACUTECTT

M

a kit for making the 99 mTc-radiolabeled peptide, apcitide, was approved for sale in the US as a radiopharmaceutical product for imaging acute deep vein thrombosis (DVT). The commercial availability of

ACUTECT

T M will significantly improve the accuracy of detection of acute DVT, and consequently, treatment of such thrombi. However, no radiopharmaceutical has thus far been approved for detection of other kinds of thrombi, and the most accurate methods available for detection of other venous thrombi such as pulmonary emboli and of arterial thrombi are invasive. Additional non-invasive agents capable of detecting the various kinds of thrombi are needed.

99mTc-radiolabeled apcitide binds to the GPIIb/IIIa receptor, the most abundant glycoprotein on the surface of platelets. The GPIIb/lIa receptor is required for platelet aggregation and is a critical component of thrombus formation, functioning as the receptor for the adhesive proteins fibrinogen (the precursor of fibrin), fibronectin, von Willebrand factor, and vitronectin. The interaction between GPIIb/IIa and its natural ligands is mediated by the tripeptide sequence arginine-glycine-aspartic acid (RGD). Apcitide contains the tripeptide -L-[S-(3-aminopropyl)cysteine]-glycineaspartic acid-, which is believed to interact with GPIIb/IIa.

U.S.Pat.No. 5,645,815 discloses that high quality thrombus imaging agents comprise GPIIb/IIa receptor binding compounds which are capable of inhibiting platelet aggregation with an IC 50 less than about 0.3 pM. U.S.Pat.No. 5,830,856 discloses that such imaging agents may comprise GPIb/IIa receptor binding compounds which are capable of inhibiting platelet aggregation with an ICso less than about 1.0 gM.

One class of GPIIb/Ifla binding platelet aggregation inhibitors, disclosed in U.S.Pat.Nos. 5,403,836; 5,493,020; 5,565,449; 5,663,166; 5,674,863; 5,674,865; 5,705,890; and 5,716,951, are substituted benzodiazepinediones. The benzodiazepinedione scaffold approximates the "cupped" configuration of the RGD tripeptide, which correlates with platelet aggregation inhibitory activity. U.S.Pat.Nos.

5,403,836; 5,493,020; 5,565,449; 5,663,166; 5,674,863; 5,674,865; 5,705,890; and 5,716,951 describe several large classes of benzodiazepinedione derivatives, including derivatives of the general formula:

I

WO 00/61195 PCTUS00/10093

R

5 C0 2

R

4 LN'

D

R1 2 O R R where R and R 4 are each independently H or a reactive group; R 3 and R 5 are each independently H, alkyl, substituted alkyl, aryl, substituted aryl, or a combination thereof; D is hydrogen, phenyl, or lower alkyl; L' is a linking moiety; and Q' is a positively charged nitrogen-containing moiety. The substituted benzodiazepinediones of U.S.Pat.Nos. 5,403,836; 5,493,020; 5,565,449; 5,663,166; 5,674,863; 5,674,865; 5,705,890; and 5,716,951 are exclusively described as therapeutic agents.

Ku, et al. (1993) J. Am. Chem .Soc. 115, 8861-8862 describes design and synthesis of benzodiazepine derivatives useful as inhibitors of glycoprotein /IIbIa receptor mediated platelet aggregation. The 1,4-benzodiazepine derivatives of Ku, et al. are exclusively described as potential antithrombotic agents.

U.S.Pat.No. 4,656,026 describes spin-labeled benzodiazepines for magnetic resonance imaging of brain tissue. U.S.Pat.No. 4,777,169 discloses radioiodinated benzodiazepines used in radioimmunoassays to determine benzodiazepine levels in body fluids. U.S.Pat.No. 4,885,152 describes radioiodinated and radiobrominated benzodiazepine derivatives for detection of benzodiazepine receptors in cerebral diseases. U.S.Pat.No. 4,997,771 discloses 3 H-benzodiazepines used to assay benzodiazepine receptor binding activity. U.S.Pat.No. 5,096,695 discloses radioiodinated benzodiazepine derivatives for use as brain imaging agents. WO 95/12610 discloses N-alkyl peptide chelators which may be covalently linked to a variety of ligands, including benzodiazepines, for use in complexing rhenium or technetium ions. JP 5-310711 discloses N-substituted benzodiazepin-2-one derivatives for electron spin resonance imaging of benzodiazepine receptors in brain nerves to diagnose epilepsy, parkinsonism, cerebral ischemia and cerebral edema.

WO 00/61195 PCTfUS00/10093 SUBMMARY OF THE INVENTION The present inventors have found that benzodiazepine derivatives may be employed as effective thrombus imaging agents.

In one embodiment, the invention provides a compound comprising a glycoprotein lb/lIla receptor binding benzodiazepine derivative covalently linked to a metal ion chelator, wherein the compound retains substantial potency, as measured in a standard assay for inhibition of platelet aggregation.

In another embodiment, the invention provides a compound having a formula:

NH

H

2 N

CO

2

H

0- N 0 0

M

wherein M is a metal ion chelator.

In another embodiment, the invention provides a scintigraphic imaging agent comprising a y-emitting radionuclide and a compound of the invention.

In another embodiment, the invention provides a complex of a y-emitting radionuclide and a compound of the invention.

In yet another embodiment, the invention provides a 99 Tc chelate of a compound comprising a glycoprotein IIb/ia receptor binding benzodiazepine covalently linked to a metal ion chelating moiety, wherein the compound retains substantial potency for inhibition of human platelet aggregation, as measured in a standard inhibition of platelet aggregation assay, when chelated to 99 mTc, and wherein the chelate contains at least one sulfur ligand bound to 9 9 Tc.

In another embodiment, the invention provides a method of detecting a thrombus in a mammalian body, comprising the steps of administering an effective diagnostic amount of the scintigraphic imaging agent or complex of the invention to the body and detecting radiation localized at the thrombus.

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WO 00/61195 PCT/US00/10093 DETAILED DESCRIPTION OF THE INVENTION The patent and scientific literature referenced herein establish the knowledge available to those with skill in the art. The issued U.S. patents and allowed applications are hereby incorporated by reference.

The compounds of the invention comprise a glycoprotein UIb/Ila receptorbinding benzodiazepine moiety and a metal ion chelating moiety. In accordance with the invention, the terms "benzodiazepine derivative" or "benzodiazepine moiety" are interchangeable and are defined as including any molecule comprising the benzodiazepine nucleus, a seven-membered ring fused to an aromatic sixmembered ring. The compounds of the invention may comprise any benzodiazepine moiety, so long as the compound retains substantial potency, as measured by the inhibitory concentration at 50% (ICso) of the compound for human platelet aggregation in a standardized assay of inhibition of platelet aggregation, such as the assay set forth in Zucker, Methods in Enzymology (1989) 169, 117-133. As defined herein, "substantial potency" is defined as: preferably, an ICso for inhibition of human platelet aggregation of less than about 1 aM; more preferably, an ICso for inhibition of human platelet aggregation of less than about 0.3 iM; and most preferably, an ICso for inhibition of human platelet aggregation of less than about 0.1 tM.

Preferably, the substituted 1,4-benzodiazepine derivatives disclosed in Ku et al., supra, are used as the benzodiazepine moiety of the compounds of the invention, so long as the substituted benzodiazepine can be further derivatized for covalent attachment of a metal ion chelator without substantially affecting the platelet aggregation inhibitory activity of the benzodiazepine. Similarly, any of the substituted benzodiazepinediones disclosed in U.S.Pat.Nos. 5,403,836; 5,493,020; 5,565,449; 5,663,166; 5,674,863; 5,674,865; 5,705,890; and 5,716,951 is employed as the benzodiazepine moiety of the compounds of the present invention, so long as the substituted benzodiazepinedione can be further derivatized for covalent attachment of a metal ion chelator without substantially affecting the platelet aggregation inhibitory activity of the benzodiazepinedione. More preferably, the substituted 1,4disclosed and claimed in U.S.Pat.No. 5,663,166, are used as glycoprotein IIb/IIa binding moieties in the compounds of the invention. Most WO 00/61195 PCT/US00/10093 preferably, substituted 1,4-benzodiazepine-2,5-diones corresponding to the formulae set forth below are used as glycoprotein IIb/IIIa binding moieties in the compounds of the invention.

In one embodiment, the invention provides a compound having a formula:

R

6

CO

2

H

Q

O

L N R S R 2 1 3 O M

R

where R' is C 1

-C

8 lower alkyl, R ,R 3

R

4

R

5 and R 6 are each independently H, Ci-C 8 lower alkyl, substituted CI-Cs alkyl, aryl, substituted aryl, or a combination thereof; L' is a linking moiety; Q is a positively charged nitrogen-containing moiety; and M is a metal ion chelator.

As defined herein, "substituted Ci-Cs alkyl" means an alkyl of the designated length substituted with a hydroxyl group, an ether, a thioether, a CI-C 8 branched hydrocarbon, a CI-C 8 straight chain hydrocarbon, an amine, a primary alkylamine, a secondary alkylamine, a primary arylamine, a secondary arylamine, a primary alkylsilicate, a secondary alklysilicate, a tertiary alkylsilicate, and the like. In accordance with the invention, "aryl" may be saturated or unsaturated and may optionally be a heterocycle. A "substituted aryl" of the invention means an aryl which may optionally be a heterocycle and which is substituted at one or more positions with a hydroxyl group, an ether, a thioether, a Ci-C 8 branched hydrocarbon, a Ci-Cs straight chain hydrocarbon, an amine, a primary alkylamine, a secondary alkylamine, a primary arylamine, a secondary arylamine, a nitro group, a halogen, a sulfonic acid, an alkylsulfonyl, a sulfonamide, and the like.

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WO 00/61195 PCT/US00/10093 In another embodiment, the invention provides a compound having a formula:

R

6

CO

2

H

1 0 R 1 N R

R

2 13 0 I R

M

where R 2 is CI-Cs lower alkyl, R',R 3

R

4

R

5 and.R 6 are each independently H, C 1 -Cs lower alkyl, substituted Ci-Cs alkyl, aryl, substituted aryl, or a combination thereof; L' is a linking moiety; Q is a positively charged nitrogen-containing moiety; and M is a metal ion chelator.

In another embodiment, the invention provides a compound having a formula: where R 3 is Ci-C lower alkyl; R',R 2

R

4

R

5 and R 6 are each independently H, Ci-Cs lower alkyl, substituted Ci-Cs alkyl, aryl, substituted aryl, or a combination thereof; L' is a linking moiety; Q is a positively charged nitrogen-containing moiety; and M is a metal ion chelator.

In another embodiment, the invention provides a compound having a formula:

R

6

CO

2

H

0 0 L' N R R4_M R N-

M

R

2 0

R

3 WO 00/61195 PCT/US00/10093 where R 4 is CI-C 8 lower alkyl; R',R 2

R

3

R

5 and R 6 are each independently H, Ci-Cs lower alkyl, substituted CI-Cs alkyl, aryl, substituted aryl, or a combination thereof; L' is a linking moiety; Q is a positively charged nitrogen-containing moiety; and M is a metal ion chelator.

In another embodiment, the invention provides a compound having a formula:

R

6 S CO 2

H

O 0 L 21 sN M

SN

R 2 13 O

R

3 where R 5 is Ci-C 8 lower alkyl; R1,R 2

R

3

R

4 and R 6 are each independently H, CI-C 8 lower alkyl, substituted Ci-C 8 alkyl, aryl, substituted aryl, or a combination thereof; L' is a linking moiety; Q is a positively charged nitrogen-containing moiety; and M is a metal ion chelator.

In another embodiment, the invention provides a compound having a formula:

M-R

6

CO

2

H

Q O R^N Li N R4

R

2 1 O

R

where R 6 is Ci-C 8 lower alkyl; R',R 2

R

3 R 4 and R 5 are each independently H, CI-C 8 lower alkyl, substituted Ci-C 8 alkyl, aryl, substituted aryl, or a combination thereof; L' is a linking moiety; Q is a positively charged nitrogen-containing moiety; and M is a metal ion chelator.

In each of the formulae set forth above, linking moiety L' is a bivalent radical containing from about 3 to about 9 methylene groups, or L' may be a bivalent radical having a length equivalent to from about 3 to about 9 methylene groups. Preferably, L' has a length equivalent to from about 4 to about 6 methylene groups. More preferably, L' has a length equivalent to about 5 methylene groups. L' preferably WO 00/61195 PCT/US00/1 0093 contains one or more sp or sp atoms and thus is constrained. In accordance with the invention, L' may contain one or more alkene, alkyne, aryl, heterocycle groups, or a functional group or groups containing N, O, or S. Preferably, L' comprises a ketone, sulfoxide, secondary amine, amide, ureido, carbamate, sulfonamide, or sulfone. More preferably, L' comprises a thioether. Most preferably, Li comprises an ether, in particular, an alkylether such as a methylether.

Positively charged moiety Q contains one or more nitrogen atoms and has a pKb sufficiently high that said atoms are at least 10% positively charged at physiological pH. In accordance with the invention, Q may comprise one or more primary, secondary, tertiary, or quaternary amines or imines either isolated or conjugated with other nitrogen atoms. Alternatively, Q may be a saturated or unsaturated (including aromatic) heterocyclic group, provided that said group bears a positive charge at physiological pH.

In accordance with the invention Q may be selected from but is not limited to such groups as: amino, imino, amidino, aminomethyleneimino, aminomethyleneamrino, iminomethylamino, guanidino, NG-aminoguanidino, alkylamino, dialkylamino, trialkylamino, alkylideneamino, pyranyl, pyrroyl, imidazolyl, pyrazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, indolizinyl, isoindolyl, 3H-indolyl, indolyl, 1Hindazolyl, purinyl, 4H-quinolizinyl, isoquinolyl, quinolyl, phthalazinyl, naphthyridinyl, quinoxalinyl, quinazolinyl, cinnolinyl, pteridinyl, 4aH-carbazolyl, carbazolyl, b-carbolinyl, phenanthridinyl, acridinyl, phenanthrolinyl, phenazinyl, phenarsazinyl, phenothiazinyl, pyrrolinyl, imridazolidinyl, imidazolinyl, pyrazolidinyl, pyrazolinyl, piperidyl, piperazinyl, indolinyl, isoindolinyl, quinuclidinyl, morpholinyl, 1,3-diazacyclohex-4-ene, and multiples thereof. Optionally, any of the nitrogencontaining heterocycles set forth above may be substituted with amino, inmino, amidino, aminomethyleneamino, iminomethylamino, guanidino, NG-amino-guanidine, alkylamino, dialkylamino, trialkylamino, or alkylidene-amino groups. Preferably, Q is a amidino or substituted amidino group.

Methods for making substituted glycoprotein IIb/Illa receptor binding benzodiazepinediones are disclosed in U.S.Pat.Nos. 5,403,836; 5,493,020; 5,565,449; 5,663,166; 5,674,863; 5,674,865; 5,705,890; and 5,716,951 and in Example 1 below.

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WO 00/61195 PCT/US00/10093 Methods for making substituted glycoprotein IIb/IIa receptor bninding benzodiazepines are disclosed in Ku et al., supra.

The compounds of the invention may comprise any metal ion chelator. The metal ion chelator may be covalently linked to the substituted benzodiazepine at any position of the benzodiazepine scaffold, so long as the presence of the chelator does not substantially interfere with the compound's ability to bind to the glycoprotein IIb/IIIa receptor. By "substantially interfere" is meant that the compound retains some potency for inhibition of human platelet aggregation, as defined above.

For example, the compounds of the invention may comprise a metal ion chelator having a formula: C(pgp)*-(aa)-C(pgp) s where (pp)S is hydrogen or a thiol protecting group and (aa) is any cc- or p-amino acid not comprising a thiol group. In a preferred embodiment, the amino acid is glycine.

Methods for making such a metal ion chelator are set forth in U.S.Pat.Nos. 5,654,272; 5,681,541; 5,788,960; and 5,811,394.

Alternatively, the compound of the invention may comprise a metal ion chelator of capable of forming an electrically neutral complex with the metal ion, as set forth in U.S.Pat.Nos. 5,720,934; 5,776,428; and 5,780,007; in allowed USSNs 08/467,791, 08/468,964; and 08/170,299; and in USSN 07/871,282. Such chelators include but are not limited to:

N

SCO-(amino acid)-cysteine-CO- I I

SX

wherein X H or a protecting group; (amino acid) any amino acid;

N

-HN-cysteine-(amino acid)-HN-CH 2

SX

wherein X H or a protecting oup; wherein X H or a protecting group;

I

WO 00/61195 PCT/US00/10093 (amino acid) any amino acid; NH N-A-CO-benzodiazepine (CR)m I

S

S-(pgp)s S-(pgp) wherein each R can be independently H, CH 3 or C 2

H

5 each (pgp) can be independently a thiol protecting group or H; m, n and p are independently 2 or 3; A is linear Ci-C 8 alkyl, substituted linear Ci-C 8 alkyl, cyclic C 3 -Cs alkyl, substituted cyclic C 3 -Cs alkyl, aryl, substituted aryl, or a combination thereof; and X is benzodiazepine; and

CR,).

NH

N-A-CH(V)NHR'

I

I

S-(pgp) S-(pgp) wherein each R is independently H, CH 3 or C 2

H

5 m, n and p are independently 2 or 3; A is linear Ci-C 8 alkyl, substituted linear Ci-Cs alkyl, cyclic C 3

-C

8 alkyl, substituted cyclic

C

3 -Cs alkyl, aryl, substituted aryl, or a combination thereof; V is H or CObenzodiazepine; R' is H or benzodiazepine; provided that when V is H, R' is benzodiazepine and when R' is H, V is CO-benzodiazepine. In accordance with the invention, the substituted derivatives in the bisamide, bisthiol formulae are defined as set forth above.

Alternatively, the compound of the invention may comprise a metal ion chelator having a formula selected from the group consisting of: diethylenetriaminepentaacetic acid (DTPA); a derivative of DTPA having a formula

(HOOCCH

2 2

N(CR

2

)(CR

2

)N(CH

2 COOH)(CR2)(CR 2

)N(CH

2

COOH)

2 where each R is independently H, Ci to C 4 alkyl, or aryl and one R is covalently linked to a bivalent linker; ethylenediaminetetraacetic acid (EDTA); a derivative of EDTA having a formula 11 WO 00/61195 PCT/US00/10093

(HOOCCH

2 2 N(C(C R2)(C )N(CH 2

COOH)

2 where each R is independently H, Ci to C 4 alkyl, or aryl and one R is covalently linked to a bivalent linker; 1,4,7,10-tetraazacyclododecanetetraacetic acid and derivatives thereof; a metal ion chelator having a formula:

((CR

2 )n N N

(CR

2 )n CR2) n N N(CR2) N

(CR

2 where n is an integer that is 2 or 3 and where each R is independently H, C 1 to C 4 alkyl, or aryl and one R is covalently linked to the benzodiazepine derivative, and desferrioxamine.

More preferably, the compounds of the invention comprise a monoamine, diamide, single thiol containing metal ion chelator such as those set forth in commonly assigned copending USSN 08/253,973. Exemplary of such metal ion chelators are chelators having the formulae: R R R OR O NH HN (R n NR'2 HS R )P R RR and R R R

OR

O NH HN- "RS R R) ,SH R'2N K IP WO 00/61195 PCT/US00/10093 wherein n, m and p are each integers that are independently 0 or 1; each R' is independently H, lower alkyl, C 2

-C

4 hydroxyalkyl, or C 2

-C

4 alkoxyalkyl, and each R is independently H or where R" is a substituted Ci-Cs alkyl not comprising a thiol group, a unsubstituted Ci-C 8 alkyl, an unsubstituted phenyl, or a substituted phenyl not comprising a thiol group, and one R or R' is L 2 where L 2 is a bivalent linker moiety linking the metal chelator to the glycoprotein IIb/lla receptor-binding benzodiazepine and wherein when one R' is L 2

NR'

2 is an amine. In this embodiment, L 2 may be a Ci-

C

6 linear alkyl group, a branched chain alkyl group, a cyclic alkyl group, a carboxylic ester, a carboxamide, a sulfonamide, an ether, a thioether, an amine, an alkene, an alkyne, a 1,2- linked, optionally substituted, benzene ring, a 1,3-linked, optionally substituted, benzene ring, a 1,4-linked, optionally substituted, benzene ring, or an amino acid, or a combination thereof. In this embodiment, R" may be a Ci-C 6 linear alkyl group; a branched alkyl group; a cyclic alkyl group; a -CqOCr-, -CqNHCr- or -CqSCrgroup, where q and r are integers each independently 1 to 5 wherein the sum of q r is not greater than 6; (CI-C 6 alkyl-X, where X is a hydroxyl group, a substituted amine, a guanidine, an amidine, a substituted thiol group, or a carboxylic acid, ester, phosphate, or sulfate group; a phenyl group or a phenyl group substituted with a halogen, a hydroxyl group, a substituted amine, a guanidine group, an amidine group, a substituted thiol, an ether, a phosphate, a sulfate; an indole group; a CI-C 6 heterocyclic group containing 1 to 3 nitrogen, oxygen or sulfur atoms, or a combination thereof. In accordance with the invention, the substituted derivatives in the monoamine, diamide, thiol-containing chelator formulae are defined as set forth above.

Most preferably, the compounds of the invention comprise a metal ion chelator comprising a single thiol-containing group of formula:

C(R"R

b n-X wherein A is H, HOOC-, H2NOC-, -NHOC-, -OOC-, Re 2 NOC-, or Rd; B is H, SH, -NHRc, -N(RC) or Rd; Z is H or Rd; X is SH, -NHRc, or Rd; Ra, Rb, R and Rd are independently H, straight chain Ci-Cs alkyl, branched chain CI-Cs alkyl, or cyclic

C

3

-C

8 alkyl; n is 0, 1 or 2; Re is Ci-C 4 alkyl, an amino acid, or a peptide comprising 2 to about 10 amino acids; and: where B is -NHRc or X is SH and n is 1 or 2; where X is -NHRc or -N(Rc) B is SH and n is 1 or 2; where B is H or Rd, A is HOOC-, H 2 NOC-, -NHOC-, or -OOC-, X is SH and n is 0 or 1; where A is H or WO 00/61195 PCTUS00/10093 Rd, then where B is SH, X is -NHRc or -N(Rc) and where X is SH, B is -NHRc or and n is 1 or 2; where X is H or Rd, A is HOOC-, H 2 NOC-, -NHOC-, or OOC- and B is SH; where Z is methyl, X is methyl, A is HOOC-, H 2 NOC-, NHOC-, or -OOC- and B is SH and n is 0; and where B is SH, X is not SH and where X is SH, B is not SH.

In accordance with the invention, a metal ion chelator comprising a single thiolcontaining group may have the formula: R'-CO-(amino acid)'-(amino acid) 2

-Z'

wherein (amino acid)' and (amino acid) 2 are each independently any primary a- or 3amino acid that does not comprise a thiol group, Z' is selected from the group consisting of cysteine, homocysteine, isocysteine, penicillamine, 2-mercaptoethylamine, 2mercaptopropylamine, 2-mercapto-2-methylpropylamine, and 3-mercaptopropylamine, and R' is lower (C'-C 4 alkyl, or R'-CO is an amino acid, a peptide, or (aa)-peptide; wherein when Z' is cysteine, homocysteine, isocysteine or penicillamine, Z' comprises a carbonyl group covalently linked to a hydroxyl group, a NR 3

R

4 group, wherein each of

R

3 and R 4 are independently H, a bond, lower (C'-C 4 alkyl, an amino acid or a peptide comprising from 2 to 10 amino acids; and Alternatively, a metal ion chelator comprising a single thiol-containing group may have the formula: Y-(amino acid) 2 -(amino acid)'-NHR 2 wherein (amino acid)' and (amino acid) 2 are each independently any primary ac- or 3amino acid that does not comprise a thiol group Y is selected from the group consisting of cysteine, homocysteine, isocysteine, penicillamine, 2-mercaptoacetate, 2mercaptopropionate, 2-mercapto-2-methylpropionate, 3-mercaptopropionate, and R 2 is H, a bond, lower (C'-C 4 alkyl, and NHR 2 is an amino acid, a peptide, or (aa)-peptide; wherein when Y is cysteine, homocysteine, isocysteine or penicillamine, Y comprises an amino group covalently linked to an amino acid, a peptide, or (aa)-peptide.

Any naturally occurring, modified, substituted, or altered amino acid may be used in the single-thiol chelators of the invention. As used herein, the term modified, substituted, or altered c- or p- amino acid" includes, without limitation, penicillamine (Pen); 6-aminocaproic acid (Aca); homolysine (Hly); L-{S-(3-aminopropyl)cysteine (Apc); D-amino acids such as D-phenylalanine D-tryptophan D-tyrosine (YD), WO) 00/61195 PCTUSOO/10093 and the like; L-(4-chlorophenvl)alatllfle (Cpa); 4-amrino-tetrahydrothiopyrafl- 4 carboxylic acid (Thp); 2-naphthylalanine (Nal); D-2-naphthylalalife (D-Nal); dipropyiglycine norleucine (Nie); homocysteine (Hcy); homohomocysteine (Hllc); aminoisobutyric acid (Aib); 2-armnoindan-2-carboxylic acid (Ain); 4-aminocyclohexylalanine (Achxa); 4-amninornethyl-phelylalamlne (Amf); S-(2amninoethyl)cysteine (Aec); is O-(3-am-inopropyl)serine (Aps); 2-aminobutyric acid (Abu); norvaline (Nva); 4-arnidino-phelylalalife (Amp), 2-amino suberic acid (Asu), and the like. In accordance with the invention, the carboxyl terminal amino acids of the chelators of the invention may be in carboxylic acid form or in amridated form.

For example, suitable metal ion chelators may have any of the followin~g formulae: (amino acid)'-(amino acid) 2 -cysteine-, (amino acd'-(amrino acid) 2 -isocysteine-, (amino acid)' -(amino acid) 2 -homocysteine-, (amino acid)' (amino acid) 2 -penicillamine-, (amino acid)'-(amrino acid) mercaptoethylamrine-, (amino acd'-(amnino acid)_-2-mercaptopropylan-ifle-, (amiruno acid) 1 -(amnino acid) 2 -2mercapto-2-methylpropylanififle-, (amino acid)' -(amnino acid) 2 -3-mercaptopropylamifle-, wherein the chelator is attached to either a substituted beazodiazepine or a linker group via a covalent bond with the carboxyl terminus of the chelator or a side chain on one of the amino acid groups.

Other suitable metal ion chelators include those selected from the group consisting of: -cysteine-(amino acid)-(o,P3- or f,y-diamino acid); -isocysteine -(amino acid)-(cL,p- or f,y-diamino acid); -homocysteine-(amnino acid)-(cx,j- or r,y-diamino acid); -penidillamine-(arnino acid)-(a43- or f,y-diami-ino acid); 2-mercaptoacetic acid-(am-ino acid)-(ct,p- or f,y-diamino acid); 2- or 3-mercaptopropionic acid-(amino acid)-(cti3- or f,y-diarnino acid); 2-mercapto-2-methylpropioflic acid-(amnino acid)-(a,P- or f,y-dianino acid); I I WO 00/61195 PCT/US00/10093 wherein the metal ion chelator is attached to either a substituted benzodiazepine or a linker group via a covalent bond with the amino terminus of the chelator or a side chain on one of the amino acid groups.

For example, the compounds of the invention may include metal ion chelators having a formula selected from the group consisting of: -Gly-Gly-Cys, -Gly-Gly- Cys.amide, Gly-Gly-Cys-, Cys-Gly-Gly-, -Gly-Gly-Gly-Cys (SEQ ID.NO:1), Gly-Gly-Gly-Cys.amide (SEQ. ID.NO: Arg-Gly-Cys-, -(s-Lys)-Gly-Cys-, Orn)-Gly-Cys-, -(y-Dab)-Gly-Cys-, and -(p-Dap)-Lys-Cys-, and the like. (In these formulae, it will be understood that e-Lys represents a lysine residue in which the Eamino group, rather than the typical a-amino group, is covalently linked to the carboxyl group of the adjacent amino acid to form a peptide bond; 6-Orn represents an ornithine residue in which the 5-amino group, rather than the typical a-amino group, is covalently linked to the carboxyl group of the adjacent amino acid to form a peptide bond; y-Dab represents a 2,4-diaminobutyric acid residue in which the y-amino group is covalently linked to the carboxyl group of the adjacent amino acid to form a peptide bond; and p- Dap represents a 1,3-diaminopropionic acid residue in which the p-amino group is covalently linked to the carboxyl group of the adjacent amino acid to form a peptide bond. Other abbreviations for amino acids are conventional. The designation "Cys.amide" represents the amidated form of the residue cysteine.) Methods for making metal ion chelators of the most preferred embodiment are set forth in U.S.Pat.Nos. 5,443,815; 5,807,537; 5,814,297; and 5,866,097 and in USSNs 08/236,402; 08/253,678; 08/253,973; and 08/582,134.

Those of skill will recognize that most metal ions may be chelated to the abovementioned metal ion chelators. Any metal ion capable of generating a signal label may be chelated to the benzodiazepine derivative compound of the invention, thus forming a metal ion complex with the compound of the invention. Suitable metal ions include radioactive metal ions, fluorescent metal ions, paramagnetic metal ions, heavy metals, rare earth ions suitable for use in computerized tomography, and the like. Radioactive metal ions or radionuclides are preferred. More preferably, y-emitting radionuclides such as 6Cu, 6Ga, and 99mTc, are used in the methods of the invention. Most preferably, complexes formed between, 99 mTc and the compounds of the invention are used to image thrombi.

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WO 00/61195 PCT/US00/10093 The metal ion chelator associates with the metal ion to form a chelate, and the atoms of a chelator are commonly known as "ligands". In the chelating art, ligands are atoms capable of donating electrons to form the coordinate bonds of the chelate.

In accordance with the invention, when the metal ion is 99mTc, the chelate is termed a 99 Tc-chelate". 99 mTc is a thiophilic metal, and thus the 99 mTc-chelates of the invention preferably contain at least one sulfur ligand bound via a coordinate covalent bond to the 99 Tc.

Complexes and chelates of the invention may be formed using known methods. For example, a salt of 99Tc pertechnetate may be reacted with the compound in the presence of a reducing agent such as dithionite ions, stannous ions or ferrous ions.

In this method, the most preferred reducing agent is stannous chloride. Alternatively, complexes and chelates may be formed by ligand exchange, wherein the compound of the invention is reacted with a pre-formed labile complex of 99 Tc and another compound known as a transfer ligand. In this process, any transfer ligand may be used, for example, tartrate, citrate, gluconate, glucoheptonate, or mannitol.

Thrombus imaging agents produced using the compounds of the invention are preferably administered intravenously to a living mammal, as pharmaceutical compositions. The compound of the invention is formulated as a sterile, pyrogen-free, parenterally acceptable aqueous solution which may optionally be supplied in lyophilized form and be reconstituted by the user.

The pharmaceutical composition of the invention comprise a compound of the invention in combination with a pharmaceutically acceptable diluent or a carrier such as species appropriate albumin. As used herein, a "pharmaceutically acceptable diluent or carrier" may include any and all solvents, dispersion media, antibacterial and antifungal agents, isotonic agents, enzyme inhibitors, stabilizers, and the like.

The use of such media and agents for pharmaceutically active substances is well known in the art. For example, Sodium Chloride Injection and Ringer's Injection are commonly used as diluents. The preparation of such parenterally acceptable solutions, having due regard to pH, isotonicity, stability, and the like, is within the skill in the art.

The compounds and compositions of the invention may be provided as components of kits which may include buffers, additional vials, instructions for use, I

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WO 00/61195 PCT/US00/10093 and the like. The kit of the invention comprises a sealed vial containing a predetermined quantity of the compound, and optionally, when the metal ion is technetium-99m, a reducing agent. An appropriate amount of a transfer ligand such as tartrate, citrate, gluconate, glucoheptonate or mannitol, for example, can also be included in the kit. The components of the kit may be in liquid, frozen or dry form. Preferably, kit components are provided in lyophilized form.

In accordance with the method of this invention, imaging agents produced from pharmaceutical compositions comprising the benzodiazepine derivative compounds of the invention are preferably administered intravenously in a single unit dose, either totally as a bolus or partly as a bolus followed by infusion over 1-2 hours.. The amount of solution to be injected at unit dosage is from about 0.01 mL to about 10 mL, containing about 0.01 mCi to about 100 mCi of radioactivity, preferably from about 1 mCi to about 20 mCi.. The amount of the compound in the unit dose may range from about 0.1 to about 10 mg/kg body weight, After intravenous administration, the thrombus site is monitored, for example, by radioimaging in vivo.

The following examples are shown by way of illustration .and not by way of limitation.

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WO 00/61195 PCT/US00/10093 EXAMPLE 1 Synthesis Of 1-[(carboxyglycyl-glycyl-glycyl-cysteinamide)methyl]-4-( 2 carboxyethyl)-7-[(4-amidinophenyl)methyl]- 3 ,4-dihydro-1H-1,4-benzodiazepine- Synthesis of an exemplary benzodiazepinedione imaging agent is set forth below.

A. N-Boc-5-hydroxyanthranilic acid [1] 5-Hydroxyanthranilic acid (100 g, 0.65 mol) was transferred into a 5 liter 3necked round bottom flask equipped with a mechanical stirrer. A solution of saturated sodium carbonate (1.5 liters) was added to the reaction flask with stirring.

After carbon dioxide evolution had subsided, di-t-butyldicarbonate (156.8 g, 0.72 mol) in 1.5 liters of tetrahydrofuran (THF) was added to the reaction vessel which was stirred at a rate which insured complete mixing of the resulting biphasic mixture.

The reaction mixture was stirred at room temperature for 24 hours at which time liters of ethyl ether was added and the mixture transferred to a separatory funnel. The aqueous layer was extracted with an additional 1.0 liters of ethyl ether and brought to pH 3.0 with 2 M H 3 P0 4 Product was extracted from the aqueous solution with ethyl acetate (3 x 1.0 liters). The combined organics were washed with a saturated NaCI solution, dried over Na2SO 4 filtered and concentrated in vacuo to yield N-Bocacid (153 g, 92.5 yield).

B. N-Boc-5-benzyloxyanthranilic acid, benzyl ester [2] Sodium hydride 36.2 g, 1.51 mol) was placed into a dry 5 liter 3-necked round bottom flask under an atmosphere of argon. Anhydrous dimethylformamide (DMF) (2.0 liters) was added via canula followed by the careful addition of hydroxyanthranilic acid (150 g, 0.59 mol). The reaction mixture was cooled with an ice/water bath and benzyl bromide (148 mL, 1.24 mol) was added via syringe keeping the reaction temperature below 50 0 C. After addition was complete, the reaction mixture was stirred at 45CC 50 0 C for 6 hours. At this time additional benzyl bromide (35 mL, 0.29 mol) was added and the reaction continued at 45 0 C 50 0 C for an additional 2 hours. Acetic acid (20 mL) was carefully added and the reaction I 1 WO 00/61195 PCT/US00/10093 mixture was transferred to a round bottom flask and concentrated to a volume of approximately 400 mL. Ethyl acetate (1.0 liters) was added and the resulting solution was decanted from any solids present. The flask was rinsed with additional ethyl acetate (2 x 500 mL), decanting after each rinse. The combined organics were washed with water (1.0 liter) and saturated NaC1 (500 mL). The organics were filtered and concentrated in vacuo to yield crude N-Boc-5-benzyloxyanthranilic acid, benzyl ester as a reddish brown syrup. The crude product was transferred to boiling hexanes (3 liters) and refluxed an additional 10 minutes. The solution was filtered while still hot and allowed to cool for 48 hours to yield 72 g of product (28 yield).

C. 5-Benzyloxyanthranilic acid, benzyl ester, hydrochloride [3] acid, benzyl ester (70 g, 161 mmol) was added to a 1.4 M HCl/ethyl acetate solution (prepared by the addition of methanol to acetyl chloride and subsequent dilution with ethyl acetate). The reaction mixture was stirred at room temperature for 21 hours. After cooling to 0°C, the reaction was gently stirred for an additional 2 hours. The crystalline product was filtered off and washed with 50 ml of cold ethyl acetate. Trace solvents were removed under high vacuum to yield 5-benzyloxyanthranilic acid, benzyl ester, hydrochloride (53.4 g, 86 yield).

D. N-(Carbo-t-butoxymethyl)-5-benzyloxyanthranilic acid, benzyl ester [4] A solution of saturated sodium bicarbonate (1.0 liter) was placed in a 4 liter Ehrlenmeyer flask along with 1.0 liter of ethyl acetate. The biphasic mixture was stirred at a rate that insured mixing and 5-benzyloxyanthranilic acid, benzyl ester, hydrochloride (50.0 g, 135 mmol) was added portionwise to the stirred mixture. After addition was complete, the mixture was stirred an additional 15 minutes. The layers were partitioned in a separatory funnel and the aqueous layer was extracted with ethyl acetate (500 mL). The combined organics were dried over MgS04, filtered, and concentrated in vacuo to yield the free base, which was dissolved in anhydrous DMF (700 mL) under an atmosphere of argon. 2,6-lutidine (20.0 mL, 172 mmol) was added to the solution followed by the addition of t-butyl bromoacetate (29.0 mL, 196 mmol). The reaction mixture was stirred at 70 C under an atmosphere of argon for 48 hours. The DMF was removed on a rotary evaporator under high vacuum and the

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WO 00/6119 5 PCT/US00/10093 crude product residue was partitioned between ethyl acetate (1.0 liter) and water (500 mL). The organic layer was washed with saturated NaCI (250 mL) and dried over NaSO4. The solution was filtered and concentrated in vacuo to yield crude product as a light brown solid. The product was recrystallized from 7% ethyl acetate/hexanes to yield N-(carbo-t-butoxymethyl)-5-benzyloxyanthranlic acid, benzyl ester (46.4 g, 79 yield).

E. N-(Carbo-t-butoxymethyl)-5-hydroxyanthranilic acid acid, benzyl ester (45.0 g, 101 mmol) was dissolved in 1:1 THF/ethyl acetate (1200 mL) in a 2 liter round bottom flask. The atmosphere was flushed with argon and 10% Pd/C (4.0 g) was added. The reaction atmosphere was replaced with hydrogen gas (4 purge-fill cycles) and the reaction mixture stirred under a balloon of hydrogen for 23 hours. The atmosphere was replaced with argon and the reaction mixture filtered through a pad of Celite, washing the Celite pad with methanol (250 mL). The solvents were removed in vacuo and the resulting yellow solid placed under high vacuum overnight to yield acid (27.1 g, 100% yield).

F. N-(Carbobenzyloxymethyl)-3-aminopropionic acid, ethyl ester [6] Ethyl acrylate (24.4 mL, 225 mmol) and glycine, benzyl ester, ptoluenesulfonate (50.0 g, 148 mmol) were combined in a 250 mL round bottom flask.

With stirring, triethylamine (24.8 mL, 178 mmol) was added via syringe and the reaction mixture was stirred at room temperature for 22 hours. The reaction mixture was partitioned between ethyl acetate (660 mL) and 10% aqueous Na 2

CO

3 (300 mL).

The organics were washed with water (100 mL) and saturated NaCl (100 mL). The organics were dried over MgS04, filtered and concentrated in vacuo followed by pumping under high vacuum to yield N-(carbobenzyloxymethyl)-3-aminopropionic acid, ethyl ester (39.3 g, 73% yield) as a yellow oil.

WO 00/61195 PCT/US00/10093 SF. 1-(Carbo-t-butoxymethyl)-4-(2-carboethoxyethyl)-7-hydroxy-3,4-dihydro- O 1H-1,4-benzodiazepine-2,5-dione [7] acid (26.7 g, 100 mmol) was placed in a dry 2 liter round bottom flask. The reaction atmosphere was flushed with argon and N-(carbobenzyloxymethyl)-3-aminopropionic acid, ethyl ester (29.0 g, 109 mmol) in anhydrous DMF (500 mL) was added, followed by the addition of 0-(7azabenzotriaol- -yl)-1,1,3,3-tetramethyluronium hexafluorophosphate

(HATU

reagent, 39.9 g, 105 mmol). Triethylamine (42.0 mL, 301 mmol) was added and the Sreaction mixture was stirred at room temperature under a balloon of argon for 16 hours. The DMF was removed in vacuo on a rotary evaporator and the residue partitioned between ethyl acetate (1.0 liter) and saturated NaHCO 3 (500 mL). The organics were washed with water (500 mL), saturated NaCI (100 mL) and dried over MgS04. The organics were filtered, the volatiles removed in vacuo on a rotary evaporator and the residual oil pumped on under high vacuum to yield an oil. This oil was taken up in 9:3 ethyl acetate/methanol (1200 mL) and placed under an atmosphere of argon gas. 10% Pd/C (4.0 g) was added and the reaction atmosphere replaced with hydrogen gas (4 purge-fills). The reaction mixture was stirred under a balloon of hydrogen for 45 hours. The reaction atmosphere was replaced with argon and the suspension filtered through a pad of Celite, which was washed with an additional 300 mL of methanol. The volatiles were removed in vacuo and the residue was taken up in a minimum of dichloromethane and chromatographed on silica gel, eluting the column with 1:1 ethyl acetate/hexanes. Fractions containing pure product (Rf 0.17 in 1:1 ethyl acetate/hexanes) were combined and the solvents removed in vacuo to yield 1-(carbo-t-butoxymethyl)-4-(2-carboethoxyethyl)-7-hydroxy-3,4dihydro-lH-1,4-benzodiazepine- 2 ,5-dione as a white solid (20.2 g, 50% yield).

G. 1.(Carbo-t-butoxymethyl)-4-(2-carboethoxyethyl)-7-[( 4 cyanophenyl)methyl]-3,4-dihydro-lH-1,4-benzodiazepine-2,5-dione [8] 1-(Carbo-t-butoxymethyl)-4-(2-carboethoxyethyl)-7-hydroxy-3,4-dihydro-lH- 1,4-benzodiazepine-2,5-dione (19.5 g, 48 mmol), ct-bromo-p-tolunitrile (11.3 g, 58 mmol), and potassium carbonate (9.0 g, 65 mmol) were combined in a round bottom flask. Anhydrous DMF (300 mL) was added and the reaction mixture was stirred at 0 C for 4 days. The reaction mixture was filtered and the filtrate was concentrated in

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WO 00/61195 PCT/USOOil0093 vacuo on a rotary evaporator. The residue was partitioned between ethyl acetate (500 mL) and water (100 mL). The organics were washed with saturated NaC1 (50 mL) and dried over NaSO04. The volatiles were removed in vacuo on a rotary evaporator and the residue was chromatographed on silica gel, eluting the column with 5:6 ethyl acetate/hexanes. Fractions containing pure product (Rf 0.17, 5:6 ethyl acetate/hexanes) were combined and the solvents removed in vacuo on a rotary evaporator to yield l-(carbo-t-butoxymethyl)-4-(2-carboethoxyethyl)-7-[( 4 cyanophenyl)methyl]-3,4-dihydro-lH-1,4-benzodiazepine-2,5-dione (20.7 g, 83% yield).

H. 1-(Carbo-t-butoxymethyl)-4-(2-carboethoxyethyl)-7-[( 4 amidinophenyl)methyl]-3,4-dihydro-lH-1,4-benzodiazepine-2,5-dione, acetate [9] 1 -(Carbo-t-butoxymethyl)-4-(2-carboethoxyethyl)- 7 -[(4-cyanophenyl)methyl]- 3,4-dihydro-1H-1,4-benzodiazepine-2,5-dione (16.0 g, 31 mmol) was placed in a dry 3-necked flask equipped with an inlet tube for gas dispersion and an outlet tube connected to a trap containing 1:1 2M NaOH/Clorox bleach. The flask was flushed with argon gas and anhydrous pyridine (90 mL) was added followed by the addition of triethylamine (70 mL). The resulting solution was saturated with hydrogen sulfide gas. The inlet and outlet tubes were removed and the reaction mixture was heated at 60 0 C for 21 hours. The reaction mixture was purged with argon and the volatiles removed in vacuo on a rotary evaporator. The residue was taken up in 5:4 dichloromethane/toluene (450 mL) and this was also removed in vacuo on a rotary evaporator. The treatment with dichloromethane/toluene was repeated once more.

The residue was taken up in acetone (300 mL) and iodomethane (5.2 mL, 84 mmol) was added via syringe. The reaction mixture was stirred and heated at 60 0 C for 5.5 hours. The reaction mixture was cooled and the volatiles removed in vacuo on a rotary evaporator. The residue was taken up in anhydrous methanol (200 mL) under and atmosphere of argon and ammonium acetate (9.25 g, 120 mmol) was added. The reaction mixture was stirred at room temperature under an atmosphere of argon for 21 hours. The volatiles removed in vacuo on a rotary evaporator and the residue was taken up in acetonitrile (200 mL) and filtered through a sintered glass funnel. The reaction vessel was rinsed with additional acetonitrile (100 mL) which was also WO 00/61195 PCTUSOO/10093 filtered through the sintered glass funnel. The filtrate was concentrated in vacuc on a C1 rotary evaporator and the residue pumped on under high vacuum to yield l-(carbo-tbutoxymethyl)-4(2-carboethoxyethyl)>7( 4 amdinopIeny)metIyIJ-3 ,4-dihydro-l1H- 1,4-benzodiazepile-2,5-diofle, acetate (18.2 g,99% yield).

1. 1-(CarboxymethyI)-4(2carboethoethyl) y1)7[(4amiidihnoyl)ty]- 3,-iyr-H14bnzdaeie25doe hydrochloride 1 -(carbo-t-butoxmethyl)4(2-caboethoxvethyl> 7 4 amidinopheflyl)methylEW ,4-dihydro-l1H-i ,4-benzodiazepine-2,5-diofle, acetate (17.7 0 10 g,30 mnmol) was placed in a dry round bottom flask and 4M HCIldioxane was added N ~via syringe. The reaction mixture was stir-red at room temperature for 1.5 hours and then added dropwise to a stirred solution of an hydrous ethyl ether (1.0 liter). After addition was complete, the resulting suspension was stirred an additional 15 minutes and filtered under a blanket of argon gas. The collected solid was washed with anhydrous ethyl ether, transferred to a round bottom flask, and dried under high vacuum to yield 1 -(carboxymethyl)-4(2carboethoxyethyl) 7 4 armudinophenyl)methyl]3,4dihydrol H-i ,4-benzodiazepifle-2,5-dione, hydrochloride as a white solid (14.2 g, 93 yield). 'H NMR (CD 3 OD): 1.2-5 ppm, 3H); 2.70 ppm, (in, 2H); 3.70 ppm 4.21 ppm, (in, 4H); 4.12 2H); 4.50 ppm, 2H); 5.39 ppm, 2H); 7.20 ppm 7.40 ppm (in, 7.71 ppm 2H); 7.83 ppm 2H).

J. Fmoc-Glycyl-glycyl-gycyl-S -Tritylicysteifl-Riflk amnide resin [11] Fmoc-Rink arnide resin (12.5 g, 0.66 mnmollg, 8.25 rnmol) was sequentially coupled to N-Frnoc-S-tritylcysteine, Fmoc-glycine, Fmoc-glycine, Fmoc-glycifle, and Finoc-glycine using the following solid-phase peptide synthesis protocol: The Nterminal Fmoc group was removed by treatment of the resin with piperidinefDMIF (2 times, 5 mmn. then 15 The resin was washed with DMF (4 X 1 mmui.). The resulting resin-supported N-terminal free amine was suspended in DMEF and reacted with N-Fmoc-S-tritylcysteine (13.7 g, 23.4 mmol), which was preactivated with a 0.45M solution of 1: 1 2-l-eztizl--l-,,,, tetramethyluronium healoohsht/Nhdoyeztizl (HBTU/HOBt) in DM4F (52 mL, 23.4 rnmol) and diisopropylethylamine (8.3 mL, 47.6 mmol). The reaction time was for'2 hours. The resin was washed with DMF (3 times),

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WO 00/61195 PCT/US0/10093 dichloromethane (3 times), and DMF (3 times). Fmoc-glycine (7.0 g, 23.5 mmol) was similarly reacted with the resin supported peptide in three sequential procedures to produce Fmoc-glycyl-glycyl-glycyl-S-tritylcysteinyl-Rink amide resin. The resin was washed with dichloromethane (3 times) and dried in vacuo. Substitution analysis on a small portion of resin indicated that resin substitution was 0.13 mnmol/g.

K. 1-[(Carboxyglycyl-glycyl-glycyl-S-tritylcysteinamide)methyl]4-( 2 carboethoxyethyl)-7-[(4-amidinophenyl)methyl]-3,4-dihydro-1H-1,4trifluoroacetate [12] Fmoc-glycyl-glycyl-glycyl-S-tritylcysteinyl-Rink amide resin (53.4 g, 6.94 mmol) was treated (2 times) with 20% piperidine/DMF (75 mL, 5 min. then 15 min.) and washed with DMF (4 times). Separately, 1-(carboxymethyl)-4-(2carboethoxyethyl)-7-[(4-amidinophenyl)methyl]-3,4-dihydro- I H-1,4-benzodiazepinehydrochloride (5.9 g, 11.36 mmol) was placed in a dry round bottom flask under an atmosphere of argon and dissolved in anhydrous DMF (75 mL). This solution was stirred and cooled to 0 0 C and treated with 4-methylmorpholine (1.01 mL, 9.2 mmol) followed by the dropwise addition of isobutyl chloroformate (1.13 mL, 8.7 mmol). After addition was complete, the solution was stirred at 0 0 C for an additional minutes and added to the resin-supported peptide free amine that was generated above. Theresin suspension was agitated for 3 hours. The resin was washed with DMF (6 times) and dichloromethane (3 times). The resin was treated three times with trifluoroacetic acid (40 mL) for 10 minutes, each time draining the deprotection mixture into a round bottom flask. The resin was washed twice with dichloromethane mL). The dichloromethane washes were combined with the trifluoroacetic acid deprotection mixtures and the volatiles removed in vacuo on a rotary evaporator. The residue was treated with anhydrous chloroform several times, each time removing the chloroform in vacuo on a rotary evaporator. This is done until the orange/yellow color of the residue disappears, indicating reattachment of the trityl group to the cysteine sulfhydryl. The crude product was pumped on under high vacuum to yield 1- [(carboxyglycyl-glycyl-glycyl-S-tritylcysteinamide)methyl]-4-(2-carboethoxyethyl)- 7-[(4-amidinophenyl)methyl-3,4-dihydro-1H-1 ,4-benzodiazepine-2,5-dione, trifluoroacetate (8.4 The product was purified by reversed-phase C18 HPLC. The column was loaded with a DMF solution of crude product (70 mg/mL) and eluted WO 00/61195 PCT/US00110093 with a linear gradient of 0.1% TFA in 90% acetonitrile/water (solvent B) and 0.1% TFA in water (solvent The gradient was from 20% B/A to 45% B/A over minutes. Fractions were analyzed by reversed-phase C18 HPLC and fractions containing pure product were combined and lyophilized to yield 1-[(carboxyglycylglycvl-glycyl-S-tritylcysteinamide)methyll-4-(2-carboethoxyethyl)-7-[(4amidinophenyl)methyl]-3,4-dihydro- 1 H-1,4-benzodiazepine-2,5-dione, trifluoroacetate as a white powder (2.41 g, 2.17 mmol, 31 yield). Electrospray mass spectral analysis indicated a molecular ion peak (M If) of 998 (theoretical for

C

52

H

56

N

9 0 0

S

1 is 998.4).

L. 1-[(Carboxyglycyl-glycyl-glycyl-cysteinamide)methyl]-4-(2-carboxyethyl)- 7-[(4-amidinophenyl)methyl]-3,4-dihydro-1H-1,4-benzodiazepine-2,5dione, trifluoroacetate [13] 1-[(carboxyglycyl-glycyl-glycyl-S-tritylcysteinamide)methyl]-4-(2carboethoxyethyl)-7-[(4-amidinophenyl)methyl]-3,4-dihydro-1H-1 ,4-benzodiazepinetrifluoroacetate (2.38 g, 2.14 mmol) was placed in a round bottom flask and dissolved in methanol (100 mL). The stirred solution was treated with an aqueous solution of 1.0 M lithium hydroxide (8.7 mL, 8.7 mmol) at room temperature for hours. Trifluoroacetic acid (0.67 mL, 8.7 mmol) was added to quench the reaction and the volatiles were removed in vacuo on a rotary evaporator. The residue was treated with a 91:4:5 mixture of trifluoroacetic acid/triethylsilane/water (100 mL) for minutes. The volatiles were removed in vacuo on a rotary evaporator. The residue was dissolved in 0.1% TFA in 90% acetonitrile/water (20 mL) and the stirred solution was diluted with 0.1% TFA in water (200 mL). The resulting precipitate was filtered off through a pad of Celite, which was washed with additional 0.1% TFA in water (100 mL). The combined filtrates were purified (in portions) by preparative reversedphase C18 HPLC. The column was eluted with a linear gradient of 100% A to B/A over 40 minutes TFA in water is solvent A and 0.1% TFA in acetonitrile/water is solvent Fractions were analyzed by reversed-phase C18 HPLC and fractions containing pure product were combined and lyophilized to yield 1 -[(carboxyglycyl-glycyl-glycyl-cysteinamide)methyl]-4-(2-carboxyethyl)-7-[(4amidinophenyl)methyl]-3,4-dihydro- 1H-I ,4-benzodiazepine-2,5-dione, trifluoroacetate as a white powder (1.08 g, 12.4 nmol, 58 yield).

WO 00/61195 PCT/US00/10093 EXAMPLE 2 Placebo Vial Method for Radiolabeling with Technetium-99m Approximately 100 cg of the benzodiazepine derivative compound of Example 1 as 100 L of a 1 mg/mL TFA salt solution dissolved in 0.9% saline was added to a "placebo vial", containing lyophilized 5 mg sodium glucoheptanate dihydrate, 50 pg stannous chloride dihydrate, and 100 pg sodium edetate dihydrate.

The vial was then reconstituted with 99 mTc-sodium pertechnetate (30 to 50 mCi) and saline such that the total volume was 1.1 mL. Following reconstitution, the vials were incubated at room temperature for 30 minutes.

The purity of the 99 mTc-labeled benzodiazepinedione derivative was determined by reverse-phase analytical HPLC using the following conditions: a Waters Delta Pak C18, 5 ji, 3.9 mm x 150 mm analytical column was loaded with each radiolabeled peptide, and the peptides eluted at a solvent flow rate equal to 1.2 mL/min. Gradient elution was performed using a linear gradient of 12-25% Solvent B/Solvent A (Solvent A is 0.1% trifluoroacetic acid (TFA) in water and Solvent B is 0.1% TFA in 90/10 acetonitrile/water) over 20 minutes; followed by a linear gradient of 25-100% Solvent B/Solvent A over four minutes and 100% Solvent B/Solvent A for three minutes (Method 1).

Radioactive components were detected in the HPLC method using an in-line radiometric detector linked to a computerized data collection and analysis system (Waters Millenium). 9 9 mTc-glucoheptate, 99 mTc-edetate, and 99 Tc-sodium pertechnetate elute between one and four minutes under these conditions, whereas the 9 9 Tc-labeled benzodiazepinedione derivatives eluted after a much greater time. The radiochemical purity (as determined by the area of the main 99 mTc product peaks) was 2 The purity of the 99 'Tc-labeled benzodiazepinedione derivative was also determined by TLC quality control analysis. The radiolabeled peptide samples were spotted at the origin of each of two Gelman ITLC-SG strips. One strip each was developed in saturated saline (SAS) and 1:1 methanol:0.1 M ammonium acetate (MAM) and allowed to dry. The SAS strips were cut at Rf 0.75 and the MAM strip was cut at Rf 0.40. The portions of the strips were counted for radioactivity in a dose 27

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WO 00/61195 PCT/US00/10093 calibrator, and the per cent activity of the top and bottom portions of each strip calculated. The radiochemical purity of each sample was calculated as follows: Purity by TLC bottom (SAS) bottom (MAM) The radiochemical purity by TLC was 2 EXAMIPLE 3 Formulated Kit Method for Radiolabeling with Technetium-99m Formulated kits were prepared by combining components in an appropriate ratio in aqueous solution, adjusting the pH to pH 7.4, dispensing 1.0 mL into glass vials, and lyophilizing. The components were dissolved in aqueous solution such that one milliliter (one vial) contained the following: 50 plg of the benzodiazepinedione derivative of Example 1 with 25 mg sodium glucoheptonate dihydrate, 50 ag stannous chloride dihydrate, 100 p.g sodium edetate dihydrate, and 5 mg L-methionine. The formulated kit was reconstituted with 99 mTc-sodium pertechnetate (45 to 55 mCi) and saline such that the total volume was 1.0 mL. Following reconstitution, the formulated kit vials were incubated at 100 0 C in a boiling water bath for 10 minutes, and allowed to cool for 20 minutes at room temperature.

The purity of the 9 9 mTc-labeled benzodiazepinedione derivative was determined by reverse-phase analytical HPLC using the following conditions: a Zorbax 300SB C18, 4 u, 4.6 mm x 250 mm analytical column was loaded with each radiolabeled peptide, and the peptides eluted at a solvent flow rate equal to 1.2 mlJmin. Gradient elution was performed using a linear gradient of 23-46% Solvent D/Solvent C (Solvent C is 5 mM tetrabutylammonium phosphate pH 7.5 in water and Solvent D is 5 mM tetrabutylammonium phosphate pH 7.5 in 60/40 acetonitrile/water) over 20 minutes; followed by a linear gradient of 46-100% Solvent D/Solvent C over 5 minutes and 100% Solvent D/Solvent C for 5 minutes (Method 2).

Radioactive components were detected in HPLC Method 2 by the same detection methods described for Method 1 in Example 2. The radiochemical purity obtained from the formulated kit preparations (as determined by the area of the main 99 mTc product peaks) was 90% for >6 hours.

The purity of the 99 mTc-labeled benzodiazepinedione derivative was also determined by TLC quality control analysis as described in Example 2.

WO 00/61195 PCT/US00/10093 Results of HPLC and TLC analysis of the 99 Tc-labeled benzodiazepinedione derivative synthesized in Example 1 are shown in Table 1.

Table 1 TLC and HPLC Results for 99 "Tc-Labeled Benzodiazepinedione TLC Purity HPLC Method HPLC Retention HPLC Purity ITime (min) Example 2 99 1 10.8, 12.0 92 Example 3" 99 2 11.8, 13.7 94 100 2 11.6, 13.5 94 aTwo entries represent two different lots of formulated kits.

EXAMPLE 4 In vitro Studies 99 mTc-benzodiazepinedione derivative was prepared as described in Example 2.

Preparation of Platelet-Rich Plasma (PRP). In all experiments, platelets were isolated on the day of the experiment from citrated human blood. After obtaining informed consent, 27 mL of blood was withdrawn from the antecubital vein of healthy adult volunteers into a polypropylene syringe containing 3 mL of sodium citrate w/v, pH Universal precautions for handling biological fluids were followed. Citrated blood was transferred to a 50 mL conical centrifuge tube and centrifuged at 900 rpm (160xg) for 10 minutes to obtain PRP.

Washed Platelets. Washed platelets were prepared by centrifuging PRP at 2,200 rpm (1,400 x g) for 12 min. The platelet-poor plasma (PPP) was decanted and the resulting pellet suspended in modified Tyrodes buffer. The PPP was decanted and discarded. Modified Tyrodes buffer (0.8 mL per mL of original PRP) was immediately layered over the platelet pellet and prostaglandin E (PGEI; 1 pL of a pM solution per mL of Tyrodes buffer) added to prevent platelet activation. (McLane MA, Kowalska MA, Silver L, Shattil SJ and Niewiarowski S. (1994) Biochem J 301: 429-426). The pellet was resuspended with a plastic Pasteur pipette. The

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WO 00/61195 PCTIUSOO/10093 centrifugation step was repeated to wash the platelets that were diluted in 150 mL of Tyrodes buffer for binding assays.

Polyethyleneimine-Treated Filters. The filters (GF/C) were presoaked in mM Tris-HCI (pH 9.1) polyethyleneimine and P829 (0.001%) for at least one hour prior to assay to decrease the nonspecific binding of 99 mTc labeled benzodiazepinedione derivative to the filter.

Binding of 99 mTc-benzodiazepinedione derivative to Washed Platelets.

Platelets (125 gpL) were incubated in silanized glass tubes for 60 min at 37°C in a shaking water bath in a total volume of 250 iL of Tyrodes buffer containing 99 mTcbenzodiazepinedione derivative (50, 30, 10, 7, 5, 3, 1, 0.7, 0.5, 0.3, 0.1, and 0.07 nM final concentration). To determine the binding of 99 mTc-benzodiazepinedione derivative to "activated" platelets, a 10 L aliquot of a 0.5 mM solution of ADP iM final concentration), and a 10 uL aliquot of a 1:1 mixture of 250 mM CaC12 and MgCl2 was added to appropriate tubes. Nonspecific binding was determined by the addition of excess unlabeled benzodiazepinedione derivative (100 PM) to fully saturate the GPIIb/lla receptor. The 99 mTc-benzodiazepinedione derivative bound to the platelets was separated from the free 99 mTc-benzodiazepinedione derivative by filtration through treated GF/C Glass Fiber Filters (Cat. No. FP24-GF/C, Brandel, Gaithersburg, MD) using a Brandel Cell harvester (Cat. No. SM24T Brandel, Gaithersburg MD) connected to a vacuum source (15 to 20 mm Hg). Filters were then washed with 9 mL of 10 mM Tris-HC1 buffer at pH 7.8 (4 0 C) and counted for radioactivity in a gamma counter.

Calculations. The specific binding of 99 mTc-benzodiazepinedione derivative was calculated by subtracting the nonspecific binding in the presence of excess unlabeled benzodiazepinedione derivative from the total binding measured in the absence of excess benzodiazepinedione derivative. The data were plotted as a Scatchard Plot as described in Bylung DB and Yamamura HI, Methods for receptor binding, Ravens Press Ltd. (New York. 1990) pp. 1-32. The data points were fitted with the linear regression function in the KaleidaGraph (Synergy Software Inc.) software package. The Kd values were calculated as the negative reciprocal of the slope.

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WO 00/61195 PCT/US00/10093 Statistics. Each response variable was compared between the two factor levels in each of the two factors using a paired student's t-test from the Instat Program (GraphPad Software, San Diego, CA). The threshold p value was set to 0.05 for rejection of the null hypothesis. For the Response Variable: Kd; Level 1: Basal (no ADP); Level 2: Activated (ADP), factor levels were compared in platelets isolated from the same individual. Experiments were run simultaneously. The platelets from four subjects were used.

The average KI for the binding of 99 mTc-benzodiazepinedione derivative to resting and activated human platelets was 30.5 nM and 13.5 nM, respectively. Thus, more 99 'Tc-benzodiazepinedione derivative bound to activated platelets. The average fold-increase in binding to activated platelets over all concentrations tested was 1.8, and ranged from 1.2 to 2.3 fold. The data obtained from Scatchard plots from which the Kd values were derived are summarized in Table 2.

Table 2 Kd Values for Binding to Human Platelets (nM).

Technetium Tc 99m P424 Basal ADP Subject 1 32 Subject 2 20 12 Subject 3 47 Subject 4 23 7 Mean 30.5 6 13.5 3 These data demonstrate that a 99 Tc-benzodiazepinedione derivative produced in accordance with the invention binds with higher potency to activated platelets than to resting platelets.

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WO 00/61195 PCT/US00/10093 EXAMPLE In Vivo Imaging of Deep Vein Thrombosis using a 9 9 Tc-Labeled Benzodiazepinedione Derivative in a Canine Model Three mongrel dogs (25-351b., fasted overnight) were sedated with a combination of ketamine and aceprozamine intramuscularly and then anesthetized with sodium pentabarbital intravenously. In each animal, an 18-gauge angiocath was inserted in the distal half of the right femoral vein and an 8mm Dacron®-entwined stainless steel embolization coil (Cook Co., BIoomington IN) was placed in the femoral vein at approximately mid-femur. The catheter was removed, the wound sutured and the placement of the coil documented by X-ray. The animals were then allowed to recover overnight.

One day following coil placement, each animal was re-anesthetized, intravenous saline drips placed in each foreleg and a urinary bladder catheter inserted to collect urine.

The animal was placed supine under a gamma camera which was equipped with a lowenergy, all purpose collimator and photopeaked for 9 Tc.

The benzodiazepinedione derivative of Example 1 was labeled with 99 "Tc [185-370 mBq (5-10 mCi)] and injected sequentially into one foreleg intravenous line at its point of insertion. The second line was maintained for blood collection.

Gamma camera imaging was started simultaneously with injection. Anterior images over the heart were acquired as a dynamic study (10 second image acquisitions) over the first 10 minutes, and then as static images at 1, 2, 3 and 4 hours post-injection.

Anterior images over the legs were acquired for 500,000 counts or 20 minutes (whichever was shorter), at approximately 10-20 minutes, and at approximately 1, 2, 3 and 4 hours post-injection. Leg images were collected with a lead shield placed over the bladder.

Following the final image, each animal was deeply anesthetized with pentobarbital. Two blood samples were collected on a cardiac puncture using a heparinized syringe followed by a euthanizing dose of saturated potassium chloride solution administered by intercardiac or bolus intravenous injection. The femoral vein containing the thrombus, a similar section of vein of the contralateral (control) leg, sections of the vessel proximal to the thrombus and samples of thigh muscle were then carefully dissected out. The thrombus, coil and coil Dacron fibers were then dissected free of the vessel. The thrombus, saline-washed vessel samples, coil and coil Dacron WO 00/61195 PCTUS00/10093 fibers were separated, and each sample was placed in a pre-weighed test tube. The samples were weighed and counted in a gamma well counter in the Tc-99m channel, along with known fractions of the injected doses.

Fresh thrombus weight, percent injected dose in the thrombus and blood obtained just prior to euthanasia and thrombus/blood and thrombus/muscle ratios were determined. From the computer-stored images, thrombus/background ratios were determined by analysis of the counts/pixel measured in regions-of-interest (ROI) drawn over the thrombus and adjacent muscle. Tissue data from these experiments are shown in Table 3.

Table 3 Canine Model of Pulmonary Embolism and Deep Vein Thrombosis' Leg Thrombus Lung Thrombus Thrombus/Background- 4.2 1.3 (n=1) %ID/g Thrombus 0.050 0.020 0.15 0.048 Thrombus/Blood- 9.1 4.6 27 9 Thrombus/Muscle' 30 Thrombus/Normal Lung 29 ±9 Thrombus Weight 430 ±210 mg 30 15 mg 'Mean standard deviation 2 From analysis of image regions of interest 3 Ratio of injected dose/g (%ID/g) These results demonstrate that pulmonary emboli and deep vein thrombi can be rapidly and efficiently located in vivo using Tc-99m labeled benzodiazepinedione derivatives of the invention.

It should be understood that the foregoing disclosure emphasizes certain specific embodiments of the invention and that all modifications or equivalents thereto are within the spirit and scope of the invention as set forth in the appended claims.

P:\WPDOCS CRN\SET\Spec\l2563791.doc-17/06/05 Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated integer or group of integers or steps but not the exclusion of any other integer or group of integers or steps.

The reference to any prior art in this specification is not, and should not be taken as, an acknowledgement or any form of suggestion that the prior art forms part of the common general knowledge in Australia.

Claims (19)

1. conmround comrnons;tni a gzlycoprotcin ITcMfma rec -ptor bindingl- ben zcocazeqin,2 delrivative- cova'1lrly linbked to a metal Ion chelator, wherein the. compound retains substantial poten--cv for inhibition of human platelet agaregalion. as measure-d ir. a standard inhibition of platelet azugregation assay.

2. The compound of claim 1, having a formula: Re C0 2 H 0\ L N R 4 R N I R 2 1 0 M R3 where R' is CI-CS lower alkyl, R2 R4, R 5 and R 6 are each independently H, C 1 -C 8 lower alkyl, substituted alkyl, aryl, substituted aryl, or a combination thereof; LI is a linking moiety; Q is a positively charged nitro gen-containinga moiety; and M is a metal ii' chelator.

3. The compound of claim 1, having a formula: R 6 CO 2 ;H o 0 'NN N R R 1 N R 2 130 M where R2 is Cj-C 8 lower alkyl, R 1 .R 3. RW, R5 and R 6are each independently H, C 1 -CS lower alkyl, substituted alkyl, ary'l, substituted aryl. or a combination thereof; L, is a linking moiety; Q is a positively charged nitro gen-containing moiety-, and MN is a metal ion chelator. WO 00/61195 PCT/US00/10093

4. The compound of claim 1, having a formula: where R 3 is Ci-C 8 lower alkyl; R 4 R R, and R 6 are each independently H, CI-Cs lower alkyl, substituted alkyl, aryl, substituted aryl, or a combination thereof;L' is a linking moiety; Q is a positively charged nitrogen-containing moiety; and M is a metal ion chelator. The compound of claim 1, having a formula: where R 4 is Ci-C 8 lower alkyl; R',R 2 R 3 R 5 and R 6 are each independently H, Ci-C 8 lower alkyl, substituted alkyl, aryl, substituted aryl, or a combination thereof;L' is a linking moiety; Q is a positively charged nitrogen-containing moiety; and M is a metal ion chelator. I WO 00/61195 PCT/US00/10093 6, The compound of claim 1, having a formula: R 6 CO 2 H Q O "L N R 5 -M L N R1 N -R 4 R 2 3 O R where R 5 is Ci-C 8 lower alkyl; R ,R 2 R 3 R 4 and R 6 are each independently H, CI-C 8 lower alkyl, substituted alkyl, aryl, substituted aryl, or a combination thereof; L' is a linking moiety; Q is a positively charged nitrogen-containing moiety; and M is a metal ion chelator.

7. The compound of claim 1, having a formula: M-R 6 COzH Q, N R R 1 N 4 R 4 R 2 R3 0 R where R 6 is CI-C 8 lower alkyl; R',R 2 R 3 R 4 and R 5 are each independently H, Ci-C 8 lower alkyl, substituted alkyl, aryl, substituted aryl, or a combination thereof; L' is a linking moiety; Q is a positively charged nitrogen-containing moiety; and M is a metal ion chelator.

8. The compound of claim 1, wherein the chelator comprises a single thiol-containing group.

9. The compound of claim 8, wherein the single thiol-containing group has a formula: A-CZ(B C(RaRb) }-X wherein A is H, HOOC-, HZNOC-, -NHOC-, -OOC-, Re2NOC-, or Rd; B is H, SH, WO 00/61195 PCT/US00/10093 -NHR', -N(Rc or Rd; Z is H or Rd; X is SH, -NHRc, -N(R) or Rd; Ra, R b R' and Rd are independently H, straight chain CI-C 8 alkyl, branched chain Ci-C 8 alkyl, or cyclic C 3 -Cg alkyl; n is 0, 1 or 2; R' is C 1 -C 4 alkyl, an amino acid, or a peptide comprising 2 to about 10 amino acids; and: where B is -NHRc or X is SH and n is 1 or 2; where X is -NHR' or B is SH and n is 1 or 2; where B is H or Rd, A is HOOC-, H 2 NOC-, -NHOC-, or -OOC-, X is SH and n is 0 or 1; where A is H or Rd, then where B is SH, X is -NHRc or and where X is SH, B is -NHR' or and n is 1 or 2; where X is H or Rd, A is HOOC-, H 2 NOC-, -NHOC-, or OOC- and B is SH; where Z is methyl, X is methyl, A is HOOC-, H 2 NOC-, NHOC-, or -OOC- and B is SH and n is 0; and where B is SH, X is not SH and where X is SH, B is not SH. A compound having a formula: NH H 2 N -0 CO 2 H C02H M wherein M is a metal ion chelator.

11. The compound of claim 10, wherein M is selected from the group consisting of: a) R'-CO-(amino acid)-(amino acid) 2 -Z l wherein (amino acid)' and (amino acid) 2 are each independently any primary a- or P- amino acid that does not comprise a thiol group, Z' is selected from the group consisting of cysteine, homocysteine, isocysteine, penicillamine, 2-mercaptoethylamine, 2- Wo OW61195 WO 00/61195PCTT'SOO/ 10093 mercaptopropyian-iine, 2-mercapto-2-methyipropylarmne, and 3-mercaptopropylarnine, and R1 is lower (CI-C 4 alkyl, or R'-CO is an amino acid, a peptide, or (aa)-peptide; wherein when Z' is cysteine, homocysteine, isocysteine or penicillamine, Z' comprises a carbonyl group covalently linked to a hydroxyl group, a NR 3R 4 group, wherein each of R 3 and R' are independently H, a bond, lower 1C_4 alkyl, an amino acid or a peptide comprising from 2 to 10 amino acids; and b) Y-(arnino acid) 2 -(ainino acid)'-NHR 2 wherein (amino acid)' and (amino acid) 2 are each independently any primary a- or amino acid that does not comprise a thiol group Y is selected from the group consisting of cysteine, homocysteine, isocysteine, penicillamine, 2-mercaptoacetate, 2- mercaptopropionate, 2-mercapto-2-methylpropionate, 3-mercaptopropionate, and R 2 is H, a bond, lower (C'-C 4 alkyl, and NHR2 is an amino acid, a peptide, or (aa)-peptide; wherein when Y is cysteine, homocysteine, isocysteine or penicillamine, Y comprises an amino group covalently linked to an amino acid, a peptide, or (aa)-peptide.

12. The compound of claim 10, wherein M is selected from the group consisting of: -Oly-Gly-Cys, -Gly-Gly-Cys.amide, Gly-Gly-Cys-, Cys-Gly-Gly-, -Gly-Gly-Gly-Cys (SEQ lID NO: -Gly-G ly-Gly-Cys.amide (SEQ DD NO:l1), Arg- Gly-Cys-, -(Fs-Lys)-Gly-Cys-, -(6-Orn)--Gly-Cys-, -(y-Dab)-Gly-Cys-, and-(- Dap)-Lys-Cys-

13. The compound of claim 10, wherein M is -Gly-Gly-Gly-Cys.amide (SEQ ID NO:l1).

14. A pharmaceutical composition comprising l-[(carboxyglycyl-glycyl-glycyl- cysteinamride)methyll-4-(2--carboxyethyl)-7-Ii(4-amridinophenyl)methyll -3 ,4-dihydro- 1 H- 1,4-benzodiazepine-2,5-dione trifluoro acetate. The composition of claim 14, further comprising 99 mTc. I WO 00/61195 PCT/US00/10093

16. A scintigraphic imaging agent comprising a y-emitting radionuclide and a compound comprising a glycoprotein Ib/Ila receptor binding benzodiazepine covalently linked to a metal ion chelating moiety, wherein the compound retains substantial potency for inhibition of human platelet aggregation, as measured in a standard inhibition of platelet aggregation assay.

17. The agent of claim 16, wherein the radionuclide is 99 mTc.

18. A complex of a y-emitting radionuclide and a compound comprising a glycoprotein Ib/IIIa receptor binding benzodiazepine covalently linked to a metal ion chelating moiety, wherein the compound retains substantial potency for inhibition of human platelet aggregation, as measured in a standard inhibition of platelet aggregation assay.

19. The complex of claim 18, wherein the radionuclide is 99 mTc. A 99 "Tc chelate of a compound comprising a glycoprotein IIb/IIIa receptor binding benzodiazepine covalently linked to a metal ion chelating moiety, wherein: a) the compound retains substantial potency for inhibition of human platelet aggregation, as measured in a standard inhibition of platelet aggregation assay; and b) the chelate contains at least one sulfur ligand bound to 99 mTc.

21. A method of detecting a thrombus in a mammalian body, comprising the steps of administering an effective diagnostic amount of the composition of claim to the body and detecting radiation localized at the thrombus.

22. A method of detecting a thrombus in a mammalian body, comprising the steps of administering an effective diagnostic amount of the agent of claim 17 to the body and detecting radiation localized at the thrombus. WO OO6tt9~pCTUSOO1I)93

23.A -thod. of dezcc:in-T a tiirombuS in a mammaln-ian body, cornrising2 the ste-,s c.1 adiisinn e~ffectivc diaz-noszic a.-nount of the cCMDie:( o F ciai-m 19 to the bd;anjd drct rad:ation localizeld ai dh thomus.

24. A method of de.tecting a th-romnbus In a mamm-Ialian body, coming the stenCS of adnitnisterins: an effective diagpostic amount of the chelate of claim 20 to the body,, and detecting radiation localized at the thrombus. A kit comprising a sealed vial containing: a) a predetermrined quantity of l-[(CarbO'.yglycyvg-l~ l-cYIgYl cvsteirl ±'dc)rethyl4(2-carboxyethl)l>I[(4-airndinophe-Iyl)methyl1 -3 ,4-dihydro- 11-1-,4-benzodiazepifle-2-,5-diohC trifluoroacetate; and b) a reducing agent. DATED this 17th day of June, 2005 DIATIDE INC. AND GENENTECH INC. by their Patent Attorneys DAVIES COLLISON CAVE