AU2005202122C1 - Substituted 1,3,4-oxadiazoles and a method of reducing TNF-alpha levels - Google Patents

Description

SUBSTITUTED 1,3,4-OXADIAZOLES o AND A METHOD OF REDUCING TNF-a LEVELS SField of the Invention The present invention relates to substituted 1,3,4-oxadiazole compounds, the method of reducing levels of tumor necrosis factor a and increasing cAMP levels and treating inflammatory and autoimmune diseases and cancer in a mammal through the administration thereof, and to pharmaceutical compositions of such derivatives.

0 cBackground of the Invention 0 0 Tumor necrosis factor-a (TNFa) is a cytokine which is released primarily by cells of immune systems in response to certain immunostimulators. When administered to animals or humans, it causes inflammation, fever, cardiovascular effects, hemorrhage, coagulation, cachexia, and acute phase responses similar to those seen during acute infections, inflammatory diseases, and shock states. Excessive or unregulated TNFa production has been implicated in a number of disease conditions. These include endotoxemia and/or toxic shock syndrome [Tracey, et al., Nature 330, 662-664 (1987) and Hinshaw, et al., Circ. Shock 30, 279-292 (1990)], rheumatoid arthritis, inflammatory bowel disease, cachexia [Dezube, et al., Lancet, 335 (8690), 662 (1990)], and lupus.

TNFa concentration in excess of 12,000 pg/mL have been detected in pulmonary aspirates from Adult Respiratory Distress Syndrome (ARDS) patients [Millar, et al., Lancet 2(8665), 712-714 (1989)]. Systemic infusion of recombinant TNFa resulted in changes typically seen in ARDS [Ferrai-Baliviera, et al., Arch. Surg. 124(12), 1400-1405 (1989)].

TNFa appears to be involved in a number of bone resorption diseases, including arthritis. When activated, leukocytes will produce bone-resorption. TNFa apparently contributes to this mechanism. [Bertolini, et al., Nature 319, 516-518 (1986) and Johnson, et al., Endocrinology 124(3), 1424-1427 (1989)]. TNFa also has been shown O to stimulate bone resorption and inhibit bone formation in vitro and in vivo. through C stimulation of bsteoclast formation and activation combined with inhibition of osteoblast functions. Another compelling link with disease is the association between production 0O of TNFa by tumor or host tissues and malignancy associated hypercalcemia [Calci.

Tissue Int. (US) 46(Suppl.), S3-10 (1990)]. In Graft versus Host Reactions, increased C serum TNFa levels have been associated with major complication following acute allogenic bone marrow transplants [Holler, et al., Blood, 75(4), 1011-1016 (1990)].

0 Ci Validation of TNF-a inhibition as a clinical therapy has been demonstrated by the therapeutic use of TNF-a antibodies and soluble TNF-a receptors. TNFa blockage with monoclonal anti-TNFa antibodies has been shown to be beneficial in rheumatoid arthritis [Elliot, et al., Int. J. Pharmac. 1995 17(2), 141-145]. High levels of TNFa are associated with Crohn's disease. [von Dullemen, et al, Gastroenterology, 1995 109(1), 129-135] treatment with soluble TNFa receptor treatment gave clinical benefits Cerebral malaria is a lethal hyperacute neurological syndrome associated with high blood levels of TNFa and the most severe complication occurring in malaria patients.

Elevated levels of serum TNFa correlated directly with the severity of disease and the prognosis in patients with acute malaria attacks [Grau, et al., N. Engl. J. Med. 320(24), 1586-1591 (1989)].

TNFa plays a role in the area of chronic pulmonary inflammatory diseases. The deposition of silica particles leads to silicosis, a disease of progressive respiratory failure caused by a fibrotic reaction. Antibodies to TNFa completely blocked the silicainduced lung fibrosis in mice [Pignet, et al., Nature, 344, 245-247 (1990)]. High levels of TNFa production (in the serum and in isolated macrophages) have been demonstrated in animal models of silica and asbestos induced fibrosis [Bissonriette, et al., Inflammation 13(3), 329-339 (1989)]. Alveolar macrophages from pulmonary sarcoidosis patients have also been found to spontaneously release massive quantities o of TNFa as compared with macrophages from normal donors [Baughman, et al., J. Lab.

Clin. Med. 115(1), 36-42 (1990)].

SElevated levels of TNFa are implicated in reperfusion injury, the inflammatory 00 response which follows reperfusion, and is a major cause of tissue damage after blood flow loss [Vedder, et al., PNAS 87, 2643-2646 (1990)]. TNFa also alters the properties C of endothelial cells and has various pro-coagulant activities, such as producing an Cl increase in tissue factor pro-coagulant activity, suppressing the anticoagulant protein C 0 Spathway, and down-regulating the expression of thrombomodulin [Sherry, et al., J. Cell S Biol. 107, 1269-1277 (1988)]. TNFa has pro-inflammatory activities which together with its early production (during the initial stage of an inflammatory event) make it a likely mediator of tissue injury in several important disorders including but not limited to, myocardial infarction, stroke and circulatory shock. TNFa-induced expression of adhesion molecules, such as intercellular adhesion molecules (ICAM) or endothelial leukocyte adhesion molecules (ELAM) on endothelial cells may be especially important [Munro, et al., Am. J. Path. 135(1), 121-132 (1989)].

It has been reported that TNFa is a potent activator of retrovirus replication including activation of HIV-1. [Duh, et al., Proc. Nat. Acad. Sci. 86, 5974-5978 (1989); Poll, et al., Proc. Nat. Acad. Sci. 87, 782-785 (1990); Monto, et al., Blood 79, 2670 (1990); Clouse, et al., J. Immunol. 142, 431-438 (1989); Poll, et al., AIDS Res. Hum.

Retrovirus, 191-197 (1992)]. At least three types or strains of HIV HIV-1, HIV-2 and HIV-3) have been identified. As a consequence of HIV infection, T-cell mediated immunity is impaired and infected individuals manifest severe opportunistic infections and/or unusual neoplasms. HIV entry into the T-lymphocyte requires T-lymphocyte activation. Other viruses, such as HIV-1, HIV-2 infect T-lymphocytes after T-cell activation. This virus protein expression and/or replication is mediated or maintained by this T-cell activation. Once an activated T-lymphocyte is infected with HIV, the T- Slymphocyte must continue to be maintained in an activated state to permit HIV gene expression and/or HIV replication. Cytokines, specifically TNFa, are implicated in activated T-cell mediated HIV protein expression and/or virus replication by playing a role in 0o maintaining T-lymphocyte activation. Therefore, interference with cytokine activity such as prevention or inhibition of cytokine production, notably TNFa, in an HIV-infected indi- C vidual assists in limiting the maintenance of T-lymphocyte caused by HIV infection.

cil c- Monocytes, macrophages, and related cells, such as kupffer and glial cells, also N have been implicated in maintenance of the HIV infection. These cells, like T-cells, are targets for viral replication and the level of viral replication is dependent upon the activation state of the cells. [Rosenberg, et al., The immunopathogenesis of HIV Infection, Advances in Immunology, 57 (1989)]. Cytokines, such as TNFa, have been shown to activate HIV replication in monocytes and/or macrophages [Poli, et al., Proc. Natl. Acad.

Sci., 87, 782-784 (1990)], therefore, prevention or inhibition of cytokine production or activity aids in limiting HIV progression for T-cells. Additional studies have identified TNFa as a common factor in the activation of HIV in vitro and have provided a clear mechanism of action via a nuclear regulatory protein found in the cytoplasm of cells [Osborn, et al., PNAS 86 2336-2340]. This evidence suggests that reducing TNFa synthesis may have an antiviral effect in HIV infections, by reducing transcription and thus virus production.

AIDS viral replication of latent HIV in T-celt and macrophage lines can be induced by TNFa [Folks, et PNAS 86, 2365-2368 (1989)]. A molecular mechanism for the virus inducing activity is suggested by TNFa's ability to activate a gene regulatory protein (transcription factor, NFiB) found in the cytoplasm of cells, which promotes HIV replication through binding to a viral regulatory gene sequence (LTR) [Osborn, et al., PNAS 86, 2336-2340 (1989)]. TNFa in AIDS associated cachexia is suggested by elevated serum TNFa and high levels of spontaneous TNFa production in peripheral o blood monocytes from patients [Wright, et al., J. Immunol. 141(1), 99-104'(1988)].

TNFa has been implicated in various roles with other viral infections, such as the cytomegalia virus (CMV), influenza virus, adenovirus, and the herpes family of viruses 00 for similar reasons as those noted.

The nuclear factor KB (NFcB) is a pleiotropic transcriptional activator (Lenardo, et Ci al., Cell 1989, 58, 227-29). NFKB has been implicated as a transcriptional activator in a Cl variety of disease and inflammatory states and is thought to regulate cytokine levels including but not limited to TNFa and active HIV transcription [Dbaibo, et al., J. Biol.

0 0 Chem. 1993, 17762-66; Duh, et al., Proc. Natl. Acad. Sci. 1989, 86, 5974-78; Bachelerie, et al., Nature 1991, 350, 709-12; Boswas, et al., J. Acquired Immune Deficiency Syndrome 1993, 6, 778-786; Suzuki, et Biochem. And Biophys. Res.

Comm. 1993, 193, 277-83; Suzuki, et al., Biochem. And Biophys. Res Comm. 1992, 189, 1709-15; Suzuki, et al., Biochem. Mol. Bio. Int. 1993, 31(4), 693-700; Shakhov, et al., Proc. Natl. Acad. Sci. USA 1990, 171, 35-47; and Staal, et al., Proc. Natl. Acad. Sci.

USA 1990, 87, 9943-47]. Thus, it would be helpful to inhibit NFKB activation, nuclear translation or binding to regulate transcription of cytokine gene(s) and through this modulation and other mechanisms be useful to inhibit a multitude of disease states.

Many cellular functions are mediated by levels of adenosine monophosphate (cAMP). Such cellular functions can contribute to inflammatory conditions and diseases including asthma, inflammation, and other conditions (Lowe and Cheng, Drugs of the Future, 17(9), 799-807, 1992). It has been shown that the elevation of cAMP in inflammatory leukocytes inhibits their activation and the subsequent release of inflammatory mediators, including TNFa and NFKB. Increased levels of cAMP also lead to the relaxation of airway smooth muscle.

The primary cellular mechanism for the inactivation of cAMP is the breakdown of cAMP by a family of isoenzymes referred to as cyclic nucleotide phosphodiesterases 0 (PDE) [Beavo and Reitsnyder, Trends in Pharm., 11, 150-155, 1990]. There are ten known members of the family of PDEs. It is well documented that the inhibition of PDE type IV (PDE 4) enzyme is particularly effective in both the inhibition of inflammatory 0O mediator release and the relaxation of airway smooth muscle [Verghese, et al., Journal of Pharmacology and Experimental Therapeutics, 272(3), 1313-1320, 1995].

N

N Decreasing TNFa levels and/or increasing cAMP levels thus constitutes a valuable g therapeutic strategy for the treatment of many inflammatory, infectious, immunological, i and malignant diseases. These include but are not restricted to: septic shock, sepsis, 0 endotoxic shock, hemodynamic shock and sepsis syndrome, post ischemic reperfusion injury, malaria, mycobacterial infection, meningitis, psoriasis and other dermal diseases, congestive heart failure, fibrotic disease, cachexia, graft rejection, cancer, tumor growth, undesirable angiogenesis, autoimmune disease, opportunistic infections in AIDS, rheumatoid arthritis, rheumatoid spondylitis, osteoarthritis, other arthritic conditions, inflammatory bowel disease, Crohn's disease, ulcerative colitis, multiple sclerosis, systemic lupus erythrematosis, ENL in leprosy, radiation damage, and hyperoxic alveolar injury. Prior efforts directed to the suppression of the effects of TNFa have ranged from the utilization of steroids such as dexamethasone and prednisolone to the use of both polyclonal and monoclonal antibodies [Beutler, et al., Science 234, 470-474 (1985); WO 92/11383].

Angiogenesis, the process of new blood vessel development and formation, plays an important role in numerous normal and pathological physiological events.

Angiogenesis occurs in response to specific signals and involves a complex process characterized by infiltration of the basal lamina by vascular endothelial cells in response to angiogenic growth signal(s), migration of the endothelial cells toward the source of the signal(s), and subsequent proliferation and formation of the capillary tube. Blood flow through the newly formed capillary is initiated after the endothelial cells come into

O

O contact and connect with a preexisting capillary. Angiogenesis is required for tumor growth beyond a certain size.

Inhibitory influences predominate in the naturally occurring balance between 00 endogenous stimulators and inhibitors of angiogenesis [Rastinejad, et al., 1989, Cell 56:345-355]. In those rare instances in which neovascularization occurs under normal Ci physiological conditions, such as wound healing, organ regeneration, embryonic development, and female reproductive processes, angiogenesis is stringently regulated In and spatially and temporally delimited. Under conditions of pathological angiogenesis 0 o such as that characterizing solid tumor growth, these regulatory controls fail.

Unregulated angiogenesis becomes pathologic and sustains progression of many neoplastic and non-neoplastic diseases. A number of serious diseases are dominated by abnormal neovascularization including solid tumor growth and metastases, arthritis, some types of eye disorders, and psoriasis [Moses, et al., 1991, Biotech. 9:630-634; Folkman, et al., 1995, N. Engl J. Med., 333:1757-1763; Auerbach, et al., 1985, J.

Microvasc. Res. 29:401-411; Folkman, 1985, Advances in Cancer Research, eds. Klein and Weinhouse, Academic Press, New York, pp. 175-203; Patz, 1982, Am. J.

Opthalmol. 94:715-743; and Folkman, et 1983, Science 221:719-725. In a number of pathological conditions, the process of angiogenesis contributes to the disease state.

For example, significant data suggests that the growth of solid tumors is dependent on angiogenesis [Folkman and Klagsbrun, 1987, Science 235:442-447].

The maintenance of the avascularity of the cornea, lens, and trabecular meshwork is crucial for vision as well as for ocular physiology. See, reviews by Waltman, et al., 1978, Am. J. Ophthal. 85:704-710 and Gartner, et al., 1978, Surv. Ophthal. 22:291- 312. Currently, the treatment of these diseases, especially once neovascularization has occurred, is inadequate and blindness often results.

O

O An inhibitor of angiogenesis could have an important therapeutic role in limiting the Scontributions of this process to pathological progression of the underlying disease states as well as providing a valuable means of studying their etiology. For example, agents Sthat inhibit tumor neovascularization could play an important role in inhibiting metastatic and solid tumor growth.

C

Several kinds of compounds have been used to prevent angiogenesis. Taylor, et al.

used protamine to inhibit angiogenesis, [Taylor, et al., Nature 297:307 (1982)]. The in toxicity of protamine limits its practical use as a therapeutic. Folkman, et al. used O heparin and steroids to control angiogenesis. [Folkman, et al., Science 221:719 (1983) and U.S. Pat. Nos. 5,001,116 and 4,994,443]. Steroids, such as tetrahydrocortisol, which lack gluco and mineral corticoid activity, are angiogenic inhibitors. Interferon p is also a potent inhibitor of angiogenesis induced by allogeneic spleen cells [Sidky, et al., Cancer Research 47:5155-5161 (1987)]. Human recombinant interferon-a was reported to be successfully used in the treatment of pulmonary hemangiomatosis, an angiogenesis-induced disease [White, et al., New England J. Med. 320:1197-1200 (1989)].

Other agents which have been used to inhibit angiogenesis include ascorbic acid ethers and related compounds [Japanese Kokai Tokkyo Koho No. 58-131978]. Sulfated polysaccharide DS 4152 also shows angiogenic inhibition [Japanese Kokai Tokkyo Koho No. 63-119500]. A fungal product, fumagillin, is a potent angiostatic agent in vitro.

The compound is toxic in vivo, but a synthetic derivative, AGM 12470, has been used in vivo to treat collagen II arthritis. Fumagillin and o-substituted fumagillin derivatives are disclosed in EPO Publication Nos. 0325199A2 and 0357061A1.

In U.S. Pat. No. 5,874,081, Parish teaches use of monoclonal antibodies to inhibit angiogenesis. In W092/12717, Brem, et al. teach that some tetracyclines, particularly Minocycline, Chlortetracycline, Demeclocycline and Lymecycine are useful as inhibitors

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Sof angiogenesis. Brem, et al. teach that Minocycline inhibits angiogenesis to an extent Scomparable to that of the combination therapy of heparin and cortisone [Cancer Research, 51, 672-675, Jan. 15, 1991]. Teicher, et al. teach that tumor growth is 00 decreased and the number of metastases is reduced when the anti-angiogenic agent of metastases is reduced when the anti-angiogenic agent Minocycline is used in N conjunction with cancer chemotherapy or radiation therapy [Cancer Research, 52, C 6702-6704, Dec. 1, 1992].

0 Macrophage-induced angiogenesis is known to be stimulated by TNFa. Leibovich, o et al. reported that TNFa induces in vivo capillary blood vessel formation in the rat cornea and the developing chick chorioallantoic membranes at very low doses and suggested TNFa is a candidate for inducing angiogenesis in inflammation, wound repair, and tumor growth [Nature, 329, 630-632 (1987)].

All of the various cell types of the body can be transformed into benign or malignant tumor cells. The most frequent tumor site is lung, followed by colorectal, breast, prostate, bladder, pancreas, and then ovary. Other prevalent types of cancer include leukemia, central nervous system cancers, brain cancer, melanoma, lymphoma, erythroleukemia, uterine cancer, bone cancer, and head and neck cancer.

Cancer is now primarily treated with one or a combination of three types of therapies: surgery, radiation, and chemotherapy. Surgery involves the bulk removal of diseased tissue. While surgery is sometimes effective in removing tumors located at certain sites in the breast, colon, and skin) surgery cannot be used in the treatment of tumors located in other areas the backbone) nor in the treatment of disseminated neoplastic conditions leukemia). Chemotherapy involves the disruption of cell replication or cell metabolism. Chemotherapy is used most often in the treatment of leukemia, as well as breast, lung, and testicular cancer.

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0 Chemotherapeutic agents are often referred to as antineoplastic agents. The Salkylating agents are believed to act by alkylating and cross-linking guanine and C possibly other bases in DNA, arresting cell division. Typical alkylating agents include 00 nitrogen mustards, ethyleneimine compounds, alkyl sulfates, cisplatin, and various nitrosoureas. A disadvantage with these compounds is that they not only attack malignant cells, but also other cells which are naturally dividing, such as those of bone C marrow, skin, gastro-intestinal mucosa, and fetal tissue. Antimetabolites are typically 0 N reversible or irreversible enzyme inhibitors, or compounds that otherwise interfere with the replication, translation or transcription of nucleic acids. Thus, it would be preferable to find less toxic compounds for cancer treatment.

Matrix metalloproteinase (MMP) inhibition has been associated with several activities including inhibition of TNFa [Mohler, et al., Nature, 370, 218-220 (1994)] and inhibition of angiogenesis. MMPs are a family of secreted and membrane-bound zinc endopeptidases that play a key role in both physiological and pathological tissue degradation [Yu, et al., Drugs Aging, 1997, (3):229-244; Wojtowicz-Praga, et al., Int.

New Drugs, 16:61-75 (1997)]. These enzymes are capable of degrading the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin, and membrane glycoproteins. Ordinarily, there is a delicate balance between cell division, matrix synthesis, matrix degradation (under the control of cytokines), growth factors, and cell matrix interactions. Under pathological conditions, however, this balance can be disrupted. Conditions and diseases associated with undesired MMP levels include, but are not limited to: tumor metastasis invasion and growth, angiogenesis, rheumatoid arthritis, osteoarthritis, osteopenias such as osteoporosis, periodontitis, gingivitis, Crohn's disease, inflammatory bowel disease, and corneal epidermal or gastric ulceration.

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0 Increased MMP activity has been detected in a wide range of cancers [Denis, et al., SInvest. New Drugs, 15: 175-185 (1987)], As with TNFa, MMPs are believed to be involved in the invasive processes of angiogenesis and tumor metastasis.

00 0 0Detailed Description The present invention is based on the discovery that certain classes of nonpolypeptide compounds more fully described herein decrease the levels of TNFa, and/or inhibit PDEs particularly PDE 4, and/or inhibit angiogenesis and/or are useful in the treatment of cancer, inflammatory and autoimmune diseases. For example, compounds that selectively inhibit PDE 4 specifically would at least partially inhibit inflammation and relaxation of airway smooth muscle with a minimum of unwanted side effects, such as cardiovascular or anti-platelet effects. The compounds of the present invention are useful in the inhibition of phosphodiesterases, .particularly PDE 4, and in the treatment of disease states mediated thereby.

The compounds described herein can inhibit the action of NFKB in the nucleus and thus are useful in the treatment of a variety of diseases including but not limited to rheumatoid arthritis, rheumatoid spondylitis, osteoarthritis, other arthritic conditions, septic shock, sepsis, endotoxic shock, graft versus host disease, wasting, inflammatory bowel disease Crohn's disease, ulcerative colitis, multiple sclerosis, systemic lupus erythrematosis, ENL in leprosy, HIV, AIDS, and opportunistic infections in AIDS. TNFa and NFKB levels are influenced by a reciprocal feedback loop. As noted above, the compounds of the present invention affect the levels of both TNFa and NFKB.

o In particular, the invention pertains to 1,3,4-oxadiazole compounds of Formula 1:

RR

R2

RN-N

,N R x Formula

I

0 in which: the carbon atom designated constitutes a center of chirality; Y is C=0, CH2, SO 2 or CH 2

C=O;

X is hydrogen, or alkyl of 1 to 4 carbon atoms; each of R 2

R

3 and R 4 independently of the others, is hydrogen, halo, trifluoromethyl, acetyl, alkyl of 1 to 8 carbon atoms, alkoxy of 1 to 4 carbon atoms, nitro, cyano, hydroxy, tert-buty, -CH 2

NR"R

9

-(CH

2 2

NRR

9 or -NR 8 R9; or any two of R 2

R

3 and R 4 on adjacent carbon atoms, together with the depicted phenylene ring are naphthylidene, quinoline, quinoxaline, benzimidazole, benzodioxole or 2-hydroxybenzimidazole; each of R 5 and R 6 independently of the other, is hydrogen, alkyl of 1 to 4 carbon atoms, alkoxy of 1 to 6 carbon atoms, cyano, benzocycloalkoxy, cycloalkoxy of up to 18 carbon atoms, bicyloalkoxy of up to 18 carbon atoms, tricylcoalkoxy of up to 18 carbon atoms, or cycloalkylalkoxy of up to 18 carbon atoms; each of R 8 and R 9 taken independently of the other is hydrogen, straight alkyl of 1 to 8 carbon atoms, branched alkyl of 1 to 8 carbon atoms, phenyl, benzyl, pyridyl, pyridylmethyl, or one of R

B

and R 9 is hydrogen and the other is -COR 10 or SSO2R 0 or Ct R 8 and R 9 taken together are tetramethylene, pentamethylene,-CHNCHCH-, hexamethylene, or-CH 2

CH

2

X'CH

2

CH

2 Sin which X' is or-NH-;

R

10 is hydrogen, alkyl of 1 to 8 carbon atoms, cycloalkyl, cycloalkylmethyl of up to 6 Cq carbon atoms, phenyl, pyridyt, benzyl, imidazolylmethyl, pyridylmethyl, CH 2 NR14R 1 or NR"R 12 0 wherein R14and R 15 independently of each other, are hydrogen, methyl, I 0 ethyl, or propyl, and 0 10 wherein R" and R 1 2 independently of each other, are hydrogen, alkyl of 1 to 8 carbon atoms, phenyl, or benzyl and the acid addition salts of said compounds which contain a nitrogen atom susceptible of protonation.

It will be appreciated that while for convenience the compounds of Formula I are identified as 1,3,4-oxadiazoles. The term alkyl denotes a univalent saturated or unsaturated branched, or straight, cyclic or mixture thereof hydrocarbon chain con-taining from 1 to 8 carbon atoms. Representative of such alkyl groups are methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, cyclopentyl, and cyclopropylmethyl. Alkoxy refers to an alkyl group bound to the remainder of the molecule through an ethereal oxygen atom. Representative of such alkoxy groups are methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, sec-butoxy, tert-butoxy, cyclohexylmethoxy, and cyclopentylmethoxy.

The term cycloalkyl as used herein denotes a univalent cyclic hydrocarbon chain which may be saturated or unsaturated. Unless otherwise stated, such chains can contain up to 18 carbon atoms and include monocycloalkyl, dicycloalkyl, polycycloalkyl, and benzocycloalkyl structures. Monocycloalkyl refers to groups having a single ring group.

O Polycycloalkyl denotes hydrocarbon systems containing two or more ring systems with one or more ring carbon atoms in common; a spiro, fused, or bridged structure.

Benzocycloalkyl signifies a monocyclic alkyl group fused to a benzo group. Represen- 00 tative of monocycloalkyl groups are cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclononyl, cyclodecyl, cycloundecyl, cyclododecyl, cyclotridecyl, C cyclotetradecyi, cyclopentadecyl, cyclohexadecyl, cycloheptadecyl, and cyclooctadecyl.

SRepresentative of polycycloalky[ include decahydronaphthalene, spiro[4.5]decyl, bicy- 0 CN clo[2.2.1]heptyl, bicyclo[3.2.1]octyl, pinanyl, norbornyl, and bicyclo[22.2]octyl.

o Benzocycloalkyl is typified by tetrahydronaphthyl, indanyl, and 1.2-benzocycloheptanyl.

C Cycloalkoxy refers to a cycloalkyl group as just described, that is a monocycloalkyl, polycycloalkyl, or benzocycloalkyl structure, bound to the remainder of the molecule through an ethereal oxygen atom.

A first preferred group of compounds are those of Formula I in which Y is C=0.

A further preferred group of compounds are those of Formula I in which Y is CH 2 A further preferred group of compounds are those of Formula I in which each of R',

R

2

R

3 and R 4 independently of the others, is hydrogen, halo, methyl, ethyl, methoxy, ethoxy, nitro, cyano, hydroxy, or -NRR 9 in which each of R 8 and R 9 taken independently of the other is hydrogen or methyl or one of R" and R 9 is hydrogen and the other is -COCH 3 or COR, where R is alkyl, benzyl, pyridyl, or pyridylmethyl.

A further preferred group of compounds are those of Formula 1 in which one of R 1

R

2

R

3 and R 4 is -NH 2 or -CH 3 and the remaining of R 1

R

2

R

3 and R 4 are hydrogen.

A further preferred group of compounds are those of Formula I in which one of R

R

2

R

3 and R 4 is -NHCOCH 3

NHSO

2

R

10 or NHCOR 1 0 and the remaining of R 2

R

3 and R 4 are hydrogen.

O

0 A further preferred group of compounds are those of Formula I in which one of R 1

R

2

R

3 and R 4 is -N(CH3) 2 and the remaining of R 1

R

2

R

3 and R 4 are hydrogen.

A further preferred group of compounds are those of Formula I in which one of R 1 00

R

2

R

3 and R 4 is methyl or ethyl and the remaining of R 1

R

2

R

3 and R 4 are hydrogen.

ci A further preferred group of compounds are those of Formula I in which each of R Sand R 6 independently of the other, is methoxy, ethoxy, propoxy, cyclopentoxy, or cyclohexoxy.

o A further preferred group of compounds are those of Formula I in which R 5 is methoxy and R 6 is alkoxy, monocycloalkoxy, polycycloalkoxy, and benzocycloalkoxy.

A further preferred group of compounds are those of Formula I in which R 5 is methoxy and R 6 is ethoxy or cyclopentoxy.

The compounds of Formula 1 are used, under the supervision of qualified professionals, to inhibit the undesirable effects of TNFa and PDE 4. The compounds may also be given to treat cancer conditions, undesirable angiogenesis, inflammation, skin conditions, etc. The compounds can be administered orally, rectally, or parenterally, alone or in combination with other therapeutic agents including antibiotics, steroids, etc., to a mammal in need of treatment. Use of the terms PDE IV and PDE 4 are deemed equivalent.

The compounds can also be used topically in the treatment or prophylaxis of topical disease states including, but not limited to atopic dermatitis, psoriasis, lupus, viral infections, such as those caused by the herpes viruses, or viral conjunctivitis, psoriasis, cancer, etc. PDE 4 inhibition is a preferred embodiment, though inhibition of other phosphodiesterases is envisioned.

The compounds also can be used in the veterinary treatment of mammals other than humans in need of prevention or inhibition of TNFa production or PDE 4 inhibition.

O TNFa mediated diseases for treatment, therapeutically or prophylactically, in animals which include disease states such as those noted above. Viral infection examples include feline immunodeficiency virus, equine infectious anemia virus, caprine arthritis oo virus, visna virus, and maedi virus, as well as other lentiviruses.

Methods of preparation of acids are described in U.S. Patent No. 5,605,914 which is incorporated by reference herein. The preparation of the oxadiazoles (111) can Ci be done in a two-step fashion or in a single-pot fashion. Reaction of acid with 0 c carbonyldiimidazole (CDI) or another activating agent, followed by addition of an acyl hydrazide (NH 2 NHCXO, wherein X is a hydrogen or alkyl) provides a compound of Formula Preferred solvents for this reaction are aprotic polar solvent that include acetonitrile (CH 3 CN) tetrahydrofuran (THF), and ethyl acetate (EtOAc).

Compounds of Formula (11) can be isolated at this point. Alternatively, a compound of Formula can be used in the next reaction without isolation (a preferred solvent is then acetonitrile). In reaction dehydration of a compound of Formula (li) with dehydrating reagents such as phosphorous oxychloride (POCl 3 or phosphorous pentoxide (P 2 0 5 provides a compound of Formula (111). Heat may be used in reaction 0 0o cI a

R

6 -0

H

N

Y

H 0 b 3-- (1It) When one of R 2

R

3 and R 4 is to be amino in the final 1,3,4-oxadiazole, it often is desirable to utilize the corresponding nitro compound and then reduce the resulting nitroisoindolinone to an aminoisoindolinone after formation. Alternatively, amino groups and other.groups which may react can be converted to an appropriately protected group.

Protecting groups utilized herein denote groups which generally are not found in the final therapeutic compounds but which are intentionally introduced at some stage of the synthesis in order to protect groups which otherwise might be altered in the course of chemical manipulations. Such protecting groups are removed at a later stage of the o synthesis and compounds bearing such protecting groups thus are of importance primarily as chemical intermediates (although some derivatives also exhibit biological activity). Accordingly the precise structure of the protecting group is not critical.

O0 Numerous reactions for the formation and removal of such protecting groups are described in a number of standard works including, for example. "Protective Groups in C Organic Chemistry", Plenum Press, London and New York, 1973; Greene, Th. W.

"Protective Groups in Organic Synthesis", Wiley, New York, 1981; "The Peptides", Vol.

o 1, Schrbder and Lubke, Academic Press, London and New York, 1965; "Methoden der organischen Chemie", Houben-Weyl, 4th Edition, Vol. 15/1, Georg Thieme Verlag, Stutt- NC gart 1974, the disclosures of which are incorporated herein by reference.

The compounds of Formula I possess a center of chirality and thus can exist as optical isomers. Both the racemates of these isomers and the individual isomers themselves, as well as diastereomers when there are two chiral centers, are within the scope of the present invention. The racemates can be used as such or can be separated into their individual isomers mechanically as by chromatography using a chiral absorbent. Alternatively, the individual isomers can be prepared in chiral form or separated chemically from a mixture by forming salts with a chiral acid or base, or have such as the individual enantiomers of 10-camphorsulfonic acid, camphoric acid, abromocamphoric acid, methoxyacetic acid, tartaric acid, diacetyltartaric acid, malic acid, pyrrolidone-5-carboxylic acid, and the like, and then freeing one or both of the resolved bases, optionally repeating the process, so as obtain either or both substantially free of the other; in a form having an optical purity of Preferred examples include substantially chirally pure (R)-isomer, a substantially chirally pure (S)-isomer, or a mixture thereof, wherein the isomer is 2-[1-(3-ethoxy-4methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]isoindoline-1,3-dione, 2-[1-(3-ethoxy-4methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]benzo[e]isoindoline-1,3-dione, 0) ehx--ehxpey)2(,,-xdaol2y~tyl4mtyionoie13doe o l3ethoxy-4-methoxyphenyl)-2-(1 3,4-oxadiazol-2-yl)ethyl -methylisoindoline-1 -in,3 Ct dione, 2-fl -(3-cyclopentyloxy-4-methoxy-phenyl)-2-( 1, 3,4-oxad iazol-2-yl 00 methylisoindoline-1 ,3-dione, 2-fl -(3-cyclopentyloxy-4-methoxyphenyl)-2-( 1,3,4oxa diazoi1-2-yi) eth yij-4-methyl isoindol ine-l 3-d ione, N-[2[1 -(3-cyclopentyloxy-4c' methoxyphenyl)-2-( 1,3,4-oxadiazol-2-yl)ethyl]- 1,3-dioxoisoindolin-4-yl]acetamide, N-j2ci[1 -(3-ethoxy-4-methoxyphenyl)-2- (1 ,3,4-oxad iazol-2-yl) ethyl]-l 1,3-d ioxo isoindol in-4-yljci acetamide, 5-(tert-butyl)-2-[1 -(3-ethoxy-4-methoxyphenyl)-2-( 1,3,4-oxadiazol-2- 0 lehlionoie13doe -l(,-iehxpey)2(,,-xdaooyl)ethyl]isoindoline-1 ,3-dione, 2-[1 ,-diox-methoxyphenyl)-2-(1 ,3,4-oxadiazol-2- Ciyl)ethyljisoindolin-1 -one, 2-l -(3-ethoxy-4-methoxyphenyl)-2-(eh1 ,3,4-oxadiazol- 2yl)ethyljisoindolin-1 -one,a 2-fl -(3-ethoxy-:4-methoxyphenyl )-2-(ty1 ,3,4-oxadiazolyl)ethyl)-3-pyrrolinof3,4-]quinoline-1 ,3-dione.

The present invention als o pertains to the physiologically acceptable non-toxic acid addition salts of the compounds of Formula 1. Such salts include those derived from organic and inorganic acids such as, without limitation, hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid, methanesulphonic acid, acetic acid, tartaric acid, lactic acid, succinic acid, citric acid, malic acid, maleic acid, sorbic acid, aconitic acid, salicylic acid, phthalic acid, embonic acid, enanthic acid, and the like.

Oral dosage forms include tablets, capsules, dragees, and similar shaped, compressed pharmaceutical forms containing from 1 to 100 mg of drug per unit dosage.

Mixtures containing from 20 to 100 mgfmL can be formulated for parenteral administration which includes intramuscular, intrathecal, intravenous and intra-arterial routes of administration. Rectal administration can be effected througlt the use of suppositories formulated from conventional carriers such as cocoa butter.

O

0 cPharmaceutical compositions thus comprise one or more compounds of the present invention associated With at least one pharmaceutically acceptable carrier, dituent or excipient. In preparing such compositions, the active ingredients are usually mixed with 00 or diluted by an excipient or enclosed within such a carrier which can be in the form of a Scapsule or sachet. When the excipient serves as a diluent, it may be a solid, semi-solid, N or liquid material which acts as a vehicle, carrier, or medium for the active ingredient.

Thus, the compositions can be in the form of tablets, pills, powders, elixirs, suspensions, emulsions, solutions, syrups, soft and hard gelatin capsules, 0 o suppositories, sterile injectable solutions and sterile packaged powders. Examples of suitable excipients include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidinone polyvinylpyrrolidone, cellulose, water, syrup, and methyl cellulose, the formulations can additionally include lubricating agents such as talc, magnesium stearate and mineral oil, wetting agents, emulsifying and suspending agents, preserving agents such as methyland propylhydroxybenzoates, sweetening agents or flavoring agents.

The compositions preferably are formulated in unit dosage form, meaning physically discrete units suitable as a unitary dosage, or a predetermined fraction of a unitary dose to be administered in.a single or multiple dosage regimen to human subjects and other mammals, each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect in association with a suitable pharmaceutical excipient. The compositions can be formulated so as to provide an immediate, sustained or delayed release of active ingredient after administration to the patient by employing procedures well known in the art.

The following examples will serve to further typify the nature of thisinvention but should not be construed as a limitation in the scope thereof, which scope is defined solely by the appended claims.

O

o Example 1 2-[1 -(3-Ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl] C isoindoline-1,3-dione 00 A mixture of 3-(1,3-dioxoisoindolin-2-yl)-3-(3-ethoxy-4-methoxyphenyl)propanoic acid (3.0 g, 8.1 mmol) and carbonyldiimidazole (1.45 g, 8.94 mmol) in tetrahydrofuran mL) was stirred at room temperature for 2 hours. To the solution was added formic hydrazide (644 mg, 10.7 mmol). The mixture was stirred for 18 hours. The resulting In suspension was filtered and washed with ether. The isolated solid was stirred in a 0 0 mixture of ethyl acetate (40 mL) and water (10 mL) for 1 hour. The suspension was filtered and washed with water and ether to give crude 3-(1,3-dioxoisoindolin-2-yl)-Ncarbonylamino-3-(3-ethoxy-4-methoxyphenyl)propanamide (1.3 g, 39% yield). A solution of 3-(1,3-dioxoisoindolin-2-yI)-N-carbonylamino-3-(3-ethoxy-4-methoxyphenyl)propanamide (600 mg, 1.46 mmol) and phosphorus oxychloride (POCl 3 0.54 mL, 5.8 mmol) in acetonitrile (20 mL) was heated to reflux for 2 hours. This solution was poured into water (10 mL). The aqueous layer was extracted with ethyl acetate (2 X 50 mL).

The combined organic layers were washed with sodium hydrogen carbonate (50 mL, sat), brine (50 mL) and dried over magnesium sulfate. Removal of solvent and chromatography gave an oil. The oil was slurried in ether (10 mL). The resulting suspension was filtered to yield 2-[1-(3-ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2yl)ethyl]isoindoline-1,3-dione as a white solid (250 mg, 43% yield): mp, 132.0-134.0 °C; 'H NMR (CDC13); 6 1.46 J 6.9 Hz, 3H, CH 3 2.82 (dd, J 6.0, 15.6 Hz, 1H, CHH), 3.84 3H, CH3), 4.11 J 7.0 Hz, 2H, CH2), 4.37 (dd, J 10.3, 15.7 Hz, 1H, CHH), 5.81 (dd, J= 6.0, 10.3 Hz, 1H, NCH), 6.62 J 7.9 Hz, 1H, Ar), 7.13-7.17 2H, Ar), 7.67-7.72 2H, Ar), 7.75-7.62 2H, Ar), 8.29 1H, Ar); 3"C NMR (CDC13) 14.69, 27.70, 51.85, 55.90, 64.42, 111.32, 112.51, 120.32, 123.44, 130.14, 13163,

O

O 134.13, 148.39, 143.43, 153.03, 163.99, 167.93; Anal Calcd for C 21

H

29

N

3 0 5 C, 64.12; H, 4.87; N, 10.68. Found: C, 63.84; H, 4.90; N, 10.48.

Ct 00 Example 2 c 2-[1 -(3-Ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2yl)ethyl]benzo[e]isoindoline-1,3-dione C 2-[1-(3-Ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]benzo[e]isoindoline- In o 1,3-dione was prepared by the procedure used in Example 1. Thus, reaction of 3-(1,3dioxobenzo[elisoindolin-2-yl)-3-(3-ethoxy-4-methoxyphenyl)propanoic acid (1.50 g, 3.58 mmol), carbonyldiimidazole (0.70 g, 4.3 mmol) and formic hydrazide (310 mg, 5.16 mmol) in tetrahydrofuran (20 mL) gave crude 3-(1,3-dioxobenzo[e]isoindolin-2-yl)-Ncarbonylamino-3-(3-ethoxy-4-methoxyphenyl)propanamide (1.0 g, 2.2 mmol), which was then treated with phosphorus oxychloride (POCI 3 0.4 mL, 4.3 mmol) in acetonitrile mL). The product was obtained as a yellow solid (135 mg, 8% overall yield): mp, 139.0-141.5 OC; 1H NMR (CDCl 3 5 1.47 J 7.2 Hz, 3H, CH 3 3.85 3H, CH3), 3.87 (dd, J 6.0, 15.6 Hz, 1H, CHH), 4.13 J 6.9 Hz, 2H, CH2), 4.42 (dd, J 10.2, 15.6 Hz, 1H, CHH), 5.87 J 5.9, 10.4 Hz, 1H, NCH), 6.84 J 8.7 Hz, 1H, Ar), 7.18-7.27 2H, Ar); 7.64-7.75 2H, Ar), 7.81 J 8.3 Hz, 1H, Ar), 7.94 J 7.6 Hz, 1H, Ar), 8.14 J 8.2 Hz, 1H, Ar), 8.29 1H, CH), 8.90 J 7.5 Hz, 1H, Ar); 13C NMR (CDCl3) 8 14.63, 27.79, 51.69, 55.84, 64.39, 111.34, 112.53, 118.41, 121.22, 124.83, 126.88, 127.93, 128.62, 128.74, 129.44, 130.31, 130.87, 135.06, 136.59, 148.37,149.36, 152.95, 164.04, 168.51, 169.07; Anal Calcd for C 2 5

H

2 1

N

3 0 5

C,

67.71; H, 4.77; N, 9.48. Found: C, 67.80; H, 4.95; N, 9.20.

0 Example 3 2-[1 -(3-Ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol -2-yl)ethyl]-4methylisoindoline-1,3-dione 00 2-[1 -(3-Ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-4-methylisoindoline- 1,3-dione was prepared by the procedure of Example 1. Reaction of 3-(3-ethoxy-4- NC methoxyphenyl)-3-(4-methyl-1,3-dioxoisoindolin-2-yl)propanoic acid (2.03 g, 5.29 Ci mmol), carbonyidiimidazole (1.03 g, 6.35 mmol) and formic hydrazide (420 mg, 6.99 0 Smmol) in tetrahydrofuran (20 mL) gave crude N-carbonylamino-3-(3-ethoxy-4- 0 Smethoxyphenyl)-3-(4-methyl-1 ,3-dioxoisoindolin-2-yl)propanamide(61 0 mg, 1.43 mmol), which was then treated with phosphorus oxychloride (0.4 mL, 4.3 mmol) in acetonitrile (6 mL). The product was obtained as a white solid (311 mg, 14% overall yield): mp, 96.0-98.0 OC; "H NMR (CDC13) 8 1.47 J 6.9 Hz, 3H, CH 3 2.67 3H, CH 3 3.81 (dd, J 6.0, 15.7 Hz, 1H, CHH), 3.85 3H, CH 3 4.12 J 6.9 Hz, 2H, CH 2 4.37 (dd, J 10.2; 15.6 Hz, IH, CHH), 5.81 J 6.0, 10.3 Hz, 1H, NCH), 6.83 J 8.7 Hz, 1H, Ar), 7.14-7.17 2H, Ar), 7.43 J= 7.6 Hz, 1IH, Ar), 7.54 J= 7.3 Hz, 1H, Ar), 7.63 J 7.1 Hz, IH, Ar), 8.30 1H, CH); 1"C NMR (CDC13) 6 14.69, 17.52, 27.71, 51.62, 55.92, 64.46, 111.37, 112.63,.120.33, 121.06, 128.31, 130.33, 132.07, 133.59, 136.55, 138.18, 148.39, 149.42, 153.02, 164.08, 168.04, 168.53; Anal Calcd for C2 2

H

21

N

3 0 5 0.2 H 2 0: C, 64.29; H, 5.25; N, 10.22; H 2 0, 0.90. Found: C, 64.62; H, 5.30; N, 9.83; H 2 0, 0.71.

o Example 4 2-[1 -(3-Ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-5methylisoindoline-1,3-dione 00 2-[1 -(3-Ethoxy-4-methoxyphenyl)-2-(1 ,3,4-oxadiazol-2-yl)ethyl]-5-methylisoindoline- 1,3-dione was prepared by the procedure of Example 1. Reaction of 3-(3-ethoxy-4- Smethoxyphenyl)-3-(5-methyl-1 ,3-dioxoisoindolin-2-yl)propanoic acid (1.81 g, 4.72 N mmol), carbonyldiimidazole (0.92 g, 5.7 mmol) and formic hydrazide (375 mg, 6.2 0 S mmol) in ethyl acetate (20 mL) gave crude N-carbonylamino-3-(3-ethoxy-4- ,3-dioxoisoindolin-2-yl)propanamide (0.93 g, 2.2 mmol), which was then treated with phosphorus oxychloride (0.4 mL, 4.3 mmol) in acetonitrile (12 mL). The product was obtained as a white solid (371 mg, 19% overall yield): mp, 122.0-124.0 OC; 'H NMR (CDC13) 6 1.45 J 6.9 Hz, 3H, CH 3 2.48 3H, CH 3 3.80 (dd, J 6.0, 15.6 Hz, 1H, CHH), 3.84 3H, CH 3 4.10 J 6.9 Hz, 2H, CH 2 4.35 (dd, J= -10.3, 15.6 Hz, IH, CHH), 5.79 (dd, J= 6.0, 10.2 Hz, 1H, NCH), 6.82 J= 8.1 Hz, 1H, Ar), 7.12-7.17 2H, Ar), 7.47 J 7.5 Hz, 1H, Ar), 7.59 1H, Ar), 7.68 J= 7.6 Hz, IH, Ar), 8.28 1H, Ar); 13 C NMR (CDC 3 6 14.61, 21.86, 27.67, 51.71, 55.83, 64.36, 111.29, 112.49, 120.22, 123.27, 123.88, 128.97, 130.23, 131.95, 134.58, 145.39, 148.33, 149.34, 152.93, 163.97, 167.91, 168.04; Anal Calcd for C22121N 3 0 5

C,

64.86; H, 5.20; N, 10.31. Found: C, 64.77; H, 5.07; N, 10.30.

o Example 2-[1 -(3-Cyclopentyloxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl)-5methylisoindoline-1,3-dione 00 2-fl1-(3-Cyclopentyloxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl-5- Nmethylisoindoline-1, .3-dione was prepared by the procedure of Example 1. Reaction of N 3-(3-cyclopentyloxy-4-methoxyphenyl)-3-(5-methyl-1 ,3-dioxoisoindolin-2-yl)propanoic acid (2.33 g, 5.5 mmol), carbonyldiimidazole (1.07 g, 6.59 mmol) and formic hydrazide S(436 mg, 7.26 mmol) in ethyl acetate (20 mL) gave crude N-carbonylamino-3-(3- 0 o cyclopentyloxy-4-methoxyphenyl)-3-(5-methyl- ,3-dioxoisoindolin-2-yl)propanamide (2.24 g, 4.8 mmol), which was then treated with phosphorus oxychloride (0.9 mL, 9.6 mmol) in acetonitrile (10 mL). The product was obtained as a white solid (728 mg, 32% overall yield): mp, 184.0-186.5 'H NMR (CDC13) 8 1.55-2.00 8H, C5H8), 2.48 (s, 3H, CH 3 3.81 3H, CHa), 3.82 (dd, J 6.1, 15.7 Hz, 1H, CHH), 4.36 (dd, J 10.3, 15.7 Hz, 1H, CHH), 4.74-4.81 1IH, OCH), 5.79 (dd, J= 5.9, 10.3 Hz, 1H, NCH), 6.80 J= 8.4 Hz, 1H, Ar), 7.10 (dd, J 2.0, 8.3 Hz, 1H, Ar), 7.18 J 2.0 Hz, 1H, Ar), 7.47 J 7.5 Hz, 1H, Ar), 7.59 1H, Ar), 7;67 J 7.6 Hz, 1H, Ar), 8.28 1H, CH); 13C NMR (CDC 3 6 21.95, 24.09, 27.75, 32.77, 51.79, 56.00, 80.48, 111.73, 114.51, 120.16, 123.34, 123.95, 129.05, 130.22, 132.03, 134.65, 145.44, 147.75, 150.03, 153.00, 164.08, 167.98, 168.11; Anal Calod for C2 5

H

25

N

3 0 5 0.13 Et 2 0: C, 67.05; H, 5.80; N, 9.19. Found: C, 66.95; H, 5.88; N, 8.97. (HNMR showed the sample contained 0.13 equiv. of ether).

o Example 6 2-1 (3-Cyclopentyloxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-4methylisoindoline-1 ,3-dione 2-[1-(3-Cyclopentyloxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-4methylisoindoline-1 ,3-dione was prepared by the procedure of Example 1. Reaction of S3-(3-cyclopentyloxy-4-methoxyphenyl)-3-(4-methyl-1,3-dioxoisoindolin-2-yl)propanoic CN acid (2.23 g, 5.27 mmol), carbonyldiimidazole (0.94 g, 5.8 mmol) and formic hydrazide 0 (382 mg, 6.36 mmol) in ethyl acetate (20 mL) gave crude N-carbonylamino-3-(3o cyclopentyloxy-4-methoxyphenyl)-3-(4-methyl- ,3-dioxoisoindolin-2-yl)propanamide (1.71 g, 3.67 mmol), which was then treated with phosphorus oxychloride (0.8 mL, 8.6 mmol) in acetonitrile (10 mL). The product was obtained as a white solid (368 mg, 16% overall yield): mp, 126.0-128.5 oC; 'H NMR (CDC 3 8 1.21-1.99 SH, CsHe), 2.66 (s, 3H, CH 3 3.81 3H, CH3), 3.82 (dd, J= 6.1, 15.8 Hz, 1H, CHH), 4.37 (dd, J 10.3, 15.6 Hz, 1H, CHH), 4.76-4.83 1H, OCH), 5.80 (dd, J 5.9, 10.3 Hz, 1H, NCH), 6.81 J= 8.4 Hz, 1H, Ar), 7.09-7.18 2H, Ar), 7.43 J 7.6 Hz, 1H, Ar), 7.54 J 7.4 Hz, 1H, Ar), 7.62 J 7.1 Hz, 1H, Ar), 8.29 1H, CH); 13C NMR (CDC 3 6 17.45, 24.00, 27.67, 32.68, 51.57, 55.94, 80.44, 111.69, 114.55, 120.13, 120.98, 128.25, 130.22, 132.01, 133.50, 136.44, 138.08, 147.68, 149.99, 152.93, 164.04, 167.95, 168.56; Anal Calcd for C 25

H

25

N

3 0 5 C, 67.10; H, 5.63; N, 9.39. Found: C, 67.14; H, 5.55; N, 9.19.

0 o Example 7 -(3-CyclopentyIoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-1,3dioxoisoindolin-4-yl]acetamide 00 -(3-Cyclopentyloxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyll-1,3dioxoisoindolin-4-yl]acetamide was prepared by the procedure of Example 1. Reaction N of 3-[4-(acetylamino)-1,3-dioxoisoindolin-2-yl]-3-(3-cyclopentyloxy-4-methoxyphenyl)- Spropanoic acid (2.0 g, 4.3 mmol), carbonyldiimidazole (0.77 g, 4.8 mmol) and formic n hydrazide (314 mg, 4.7 mmol) in ethyl acetate (20 mL) gave crude 3-[4-(acetylamino)- 0 o 1,3-dioxoisoindolin-2-yl]-N-carbonylamino-3-(3-cyclopentyloxy-4-methoxyphenyl)propanamide, which was then reacted with phosphorus oxychloride (1.0 mL, 10.7 mmol) in acetonitrile (15 mL). The product was isolated as a yellow solid (555 mg, 28% overall yield): mp, 115.0-117.0 OC; 'H NMR (CDCI 3 5 1.62-1.97 8H, CsHe), 2.27 3H,

CH

3 3.76 (dd, J 5.6, 15.9 Hz, 1H, CHH), 3.83 3H, CH 3 4.40 (dd, J 10.7, 15.8 Hz, 1H, CHH), 4.76-4.82 1H, OCH), 5.78 (dd, J= 5.5, 10.7 Hz, IH, NCH), 6.84 J 8.1 Hz, IH, Ar), 7.09-7.15 2H, Ar), 7.47 J= 7.2 Hz, 1H, Ar), 7.65 J 7.5 Hz, 1H, Ar), 8.32 1H, CH), 8.76 J 8.4 Hz, 1H, Ar), 9.48 1H, NH); '3C NMR (CDC13) 8 23.99, 24.85, 27.58, 32.68, 51.71, 55.95, 80.53, 111.75, 114.46, 115.10, 118,03, 119.88, 124.82, 129.77, 130.95, 135.94, 137.48, 147.77, 150.21, 152.99, 163.85, 167.36, 169.07, 167.71; Anal Calcd for C 26

H

2 6N 4 06 0.1 hexane: C, 64.01; H, 5.53; N, 11.22. Found: C, 64.01; H, 5.58; N, 10.97. (HNMR showed the product contained 10%/ of hexane).

O

O Example 8 A> N-[2-[1-(3-Ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-1,3- Sdioxoisoindolin-4-yl]acetamide 00 A mixture of 3-[4-(acetylamino)-1,3-dioxoisoindolin-2-yl]-3-(3-ethoxy-4-methoxyphenyl)propanoic acid (1.69 g, 3.96 mmol) and carbonyldiimidazole (0.71 g, 4.4 mmol) C in acetonitrile (20 mL) was stirred at room temperature for 2 hours. To the solution was o added formic hydrazide (289 mg, 4.81 mmol). The mixture was then stirred for 18 In hours. To the resulting 'solution was added phosphorus oxychloride (1.0 mL, 10.7 0 O mmol), and this mixture was heated at reflux for 2 hours. The solution was poured to water (10 mL). The aqueous layer was extracted with ethyl acetate (2 X 50 mL). The combined organic layers were washed with aqueous sodium hydrogen carbonate mL, sat), brine (50 mL) and then dried over magnesium sulfate. Chromatography followed by removal of solvent yielded an oil. The oil was stirred in ether (10 mL) to give a suspension. This suspension was filtered to yield N-[2-[1-(3-ethoxy-4methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-1,3-dioxoisoindolin-4-yl]acetamide as a white solid (478 mg, 27% yield): mp, 141.0-143.0 OC; 1H NMR (CDCIB) 5 1.47 J 6.9 Hz, 3H, CH 3 2.26 3H, CH 3 3.74 (dd, J 5.8, 15.8 Hz, 1H, CHH), 3.85 3H,

CH

3 4.11 J 7.1 Hz, 2H, CH 2 4.38 (dd, J 10.6, 15.8 Hz, 1H, CHH), 5.78 (dd, J= 5.6, 10.6 Hz, 1H, NCH), 6.83 J 8.9 Hz, 1H, Ar), 7.11-7.14 2H, Ar), 7.45 J 7.2 Hz, 1H, Ar), 7.64 J 7.5 Hz, 1H, Ar), 8.31 1H, Ar), 8.75 J 8.4 Hz, 1H, Ar), 9.46 (br s, 1H, NH); 13C NMR (CDCI1) 8 14,70, 24.92, 27.60, 51.74, 55.92, 64.50, 111.40, 112.47, 115.15, 118.11, 120.15, 124.91, 129.87, 130.99, 136.01, 137.55, 148.49, 149.59, 153.07, 163.88, 167.44, 169.14, 169.75; Anal Calcd for C 23

H

22

N

4 06: C, 61.33; H, 4.92; N, 12.44. Found: C, 61.37; H, 4.88; N, 12.11.

0 Example 9 5-(tert-Butyl)-2-[1 -(3-ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2yl)ethyl]isoindoline-1,3-dione 00 5-(t-Butyl)-2-[1 -(3-ethoxy-4-methoxyphenyl)-2-( 1 ,3,4-oxadiazol-2yl)ethyl]isoindoline-1,3-dione was prepared as described for Example 8 from C butyl)-1,3- dioxoisoindolin-2-yl]-3-(3-ethoxy-4-methoxyphenyl)propanoic acid (2.0 g, 4.7 NC mmol) carbonyldiimidazole (0.81 g, 5.0 mmol), formic hydrazide (0.35 g, 5.8 mmol), C and phosphorus oxychloride (1.0 mL, 10.7 mmol) in acetonitrile (20 mL). The product 0 Swas isolated as a white solid (800 mg, 38% yield): mp, 136.0-138.5 oC; 'H NMR (CDC13) 5 1.35 9H, C-H3), 1.44 J= 6.9 Hz, 3H, CH3), 3.79 (dd, J 5.9, 16.1 Hz, 1H, CHH), 3.84 3H, CH 3 4.11 J= 7.1 Hz, 2H, CH 2 4.38 (dd, J= 10.3, 15.8 Hz, 1H, CHH), 5.80 (dd, J= 5.9, 10.4 Hz, 1H, NCH), 6.82 J= 8.2 Hz, 1H, Ar), 7.11-7.17 2H, Ar), 7.70 (br s, 2H, Ar), 7.82 (br s, 1H, Ar), 8.29 IH, Ar); 13C NMR (CDC13) 8 14.71, 27.73, 31.08, 35.72, 51.78, 55.92, 64.44, 111.36, 112.58, 120.31, 120.63, 123.26, 128.94, 130.33, 131.14, 131.84, 148.41, 149.42, 153.02, 158.82, 164.07, 168.25, 168.39; Anal Calcd for C2 5 sH 27

N

3 0 5 0.11 H 2 0: C, 66.51; H, 6.08; N, 9.31; H 2 0, 0.43. Found: C, 66.42; H, 5.83; N, 9,18; H 2 0, 0.43.

Example 2-[1 -(3,4-Dimethoxyphenyl)-2-(1 ,3,4-oxadiazol-2-yl)ethyl]isoindoline-1,3-d ione 2-[1 -(3,4-Dimethoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyljisoindoline-1 ,3-dione was prepared by the procedure of Example 8 from 3-(3,4-dimethoxyphenyl)-3-(1,3dioxoisoindolin-2-yl)propanoic acid (2.0 g. 3.6 mmol) carbonyldiimidazole (1.0 g, 6.2 mmol), formic hydrazide (0.41 g, 6.8 mmol), and phosphorus oxychloride (1.3 mL, 14 mmol) in acetonitrile (20 mL). The product was obtained as a white solid (730 mg, 34% yield): mp, 83.0-85.0 oC; 'H NMR (CDC1 3 6 3.82 (dd, J= 6.0, 16.0 Hz, IH, CHH), 3.85 3H, CH 3 3.90 3H, CH 3 4.39 (dd, J 10.3, 15.7 Hz, 1H, CHH), 5.84 (dd, J 10.3 Hz, 1H, NCH), 6.81-6.85 1H, Ar), 7.16-7.19 2H, Ar), 7.68-7.73 Ar), S7.77-7.83 2H, Ar), 8.30 1H, CH); "C NMR (CDC 3 5 27.66, 51.76, 55.79, 55.89, S111.00, 111.07, 120.29, 123.3,8, 130.16, 131.55, 134.07, 149.03, 149.11, 152.96, 00 163.90, 167.86; Anal Calcd for C 20 H17N 3 0 0.3 Et 2 0: C, 63.22; H, 5.20; N, 10.32.

Found: C, 63.40; H, 5.02; N, 10.46. (1H NMR showed that the sample contained 30% of CN ether).

C Example 11 C 2-[1-(3-Ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]is oindolin-1 one S2-[1-(3-Ethoxy-4-methoxyphenyl)- 2 -(1,3,4-oxadiazol-2-yl)ethyl]isoindolin-1-one was Sprepared as described in Example 1. Reaction of 3-(3-ethoxy-4-methoxyphenyl)-3-(1oxoisoindolin-2-yl)propanoic acid (1.50 g, 4.22 mmol), carbonyldiimidazole (0.80 g, 4.9 mmol) and formic hydrazide (310 mg, 5.16 mmol) in tetrahydrofuran (10 mL) yielded crude N-carbonylamino-3-(3-ethoxy-4-methoxyphenyl)-3-(1-oxoisoindolin-2-yl)propanamide (1.0 g, 2.2 mmol), which was then reacted with phosphorus pentoxide (2.32 g, 16.3 mmol) in chloroform (30 mL) at room temperature for 18 hours. The product was obtained as a white solid (250 mg, 16% overall yield): mp, 143.5-144.5

OC;

'H NMR (CDCI 3 6 1.43 J= 7.0 Hz, 3H, CH3), 3.65 (dd, J= 6.1, 15.1 Hz, 1H, CHH), 3.85 3H, CH 3 3.87 (dd, J= 9.9, 15.0 Hz, 1H, CHH), 4.01-4.12 3H, NCHH, CH 2 4.46 J= 16.6 Hz, 1H, NCHH), 5.99 (dd, J= 6.1, 10.1 Hz, 1H, NCH), 6.83-6.87,(m, 1H, Ar), 6.94-7.01 2H, Ar), 7.34-7.52 3H, Ar), 7.78 J 7.1 Hz, 1H, Ar), 8.34 1H, NCH); '3C NMR (CDC1) 6 14.60, 27.84, 46.19, 52.13, 55.86, 64.45, 111.32, 112.45, 118.98, 122.78, 123.72, 127.95, 129.95,131.49, 131.98,141.09, 148.66, 149.35, 153.31,163.86, 168.25; Anal Calcd for C2 1

H

2 1

N

3 0 4 0.06 CH2CI 2 C, 65.79; H, 5.54; N, 10.93. Found: C, 65.87; H, 5.67; N, 10.89.

o Example 12 2-[1-(3-Ethoxy-4-methoxyphenyl)-2-(5-methyl(1,3,4-oxadiazol- 2-yl))ethyl]isoindolin-1 -one 00 2-[1-(3-Ethoxy-4-methoxyphenyl)-2-(5-methyl(1, 3 4 -oxadiazol-2-yl))ethyl]isoindolin- 1-one was prepared by the procedure of Example 1. Reaction 3-(3-ethoxy-4- C- methoxyphenyl)-3-(1-oxoisoindolin-2-yl)propanoic acid (1.50 g, 4.22 mmol), N carbonyldiimidazole (0.76 g, 4.7 mmol) and acetic hydrazide (381 mg, 5.16 mmol) in

O

I tetrahydrofuran (15 mL) gave crude N-carbonylamino-3-(3-ethoxy-4-methoxyphenyl)-3- (1-oxoisoindolin-2-yl)propanamide (1.22 g, 3.06 mmol), which (650 mg, 1.47 mmol) was then reacted with phosphorus pentoxide (2.0 g, 14 mmol) in chloroform (30 mL) at room temperature for 18 hours The product was obtained as a white solid (250 mg, 32% overall yield): mp, 125.5-128.0 oC; 'H NMR (CDCb); 6 1.43 (t,J 7.0 Hz, 3H, CH 3 2.46 3H, CH3), 3.56 (dd, J= 6.3, 15.1 Hz, 1H, CHH), 3.76 (dd, J 10.0, 15.0 Hz, 1H, CHH), 3.86 3H, CH 3 4.02-4.11 3H, NCHH, CH 2 4.46 J 16.6 Hz, IH, NCHH), 5.97 (dd, J 6.3, 9.9 Hz, 1H, NCH), 6.83-6.87 1H, Ar), 6.95-7.01 2H, Ar), 7.35-7.53 3H, Ar), 7.77-7.81 1H, Ar); 13C NMR (CDC13) 6 10.89, 14.64, 28.04, 46.18, 52.08, 55.89, 64.47, 111.32, 112.51, 119.03, 122.81, 123.74, 127.95, 130.13, 131.48, 132.11, 141.17, 148.64, 149.31, 163.86, 164.23, 168.30; Anal Calcd for C22H 23

N

3 04 0.28 EtOAc: C, 66.42; H, 6.08; N, 10.05. Found: C, 66.47; H, 5.98; N, 10.04. NMR showed that the sample contained 28% of ethyl acetate).

o Example 13 N 2-[1-(3-Ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-3cJ pyrrolino[3,4]quinoline- 1,3-dione 00 2-[1-(3-Ethoxy-4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-3-pyrrolino[3,4-h]- C quinoline-1,3-dione was prepared by the procedure of Example 1. Reaction of 3-(1,3- C dioxo(3-pyrrolino[3,4-h]quinolin-2-yl))-3-(3-ethoxy-4-methoxyphenyl)propanoic acid c g, 2.4 mmol), CDI (0.46 g, 2.8 mmol) and formic hydrazide (0.20 g, 3.4 mmol) in THF mL) gave crude 3-(1,3-dioxo(3-pyrrolino[3,4-h]quinolin-2-yl))-N-carbonylamino-3-(3- Sethoxy-4-methoxyphenyl)propanamide (1.12 which was then reacted with phosphorus oxychloride (0.8 mL, 8.6 mmol) in acetonitrile (30 mL). The product was obtained as a white solid (350 mg, 33% overall yield): mp, 166-168 'H NMR

(CDCI

3 5 1.47 J 6.8 Hz, 3H, CH 3 3.85 (dd, J 5.9, 15.8 Hz, 1H, CHH), 3.85 (s, 3H, CH3), 4.13 J 6.9 Hz, 2H, CH 2 4.48 (dd, J 10.4, 15.8 Hz, 1H, CHH), 5.91 (dd, J 5.8, 10.4 Hz, 1H, NCH), 6.82-6.85 1H, Ar), 7.21-7.25 2H, Ar), 7.58 (dd, J 4.2, 8.4 Hz, 1H, Ar), 7.94 J 8.0 Hz, 1H, Ar), 8.19 J 8.2 Hz, 1H, Ar), 8.27 (dd, J 1.7, 8.4 Hz, 1H, Ar), 8.28 1H, CH), 9.24 (dd, J 1.7, 4.2 Hz, 1H); ,3C NMR (CDC13) 5 14.63, 27.60, 51.83, 55.85, 64.39, 111.29, 112.58, 119.52, 120.43, 123.16, 126.81, 130.08, 132.14, 134.44, 135.57, 136.68, 142.77, 148.34, 149.36, 152.97, 154.27, 163.99, 167.07, 167.80, Anal Calcd for C 24

H

20

N

4 0 5 0.05 CH 2

CI

2 C, 64.38; H, 4.52; N, 12.49. Found: C, 64.33; H, 4.58; N, 12.12. (H NMR showed the sample contained of CH 2

CI

2

O

0 Example 14 Tablets, each containing 50 mg of 2-[1-(3-cyclopentyloxy-4-methoxyphenyl)-2- (1,3,4-oxadiazol-2-yl)ethyl]-5-methylisoindoline-1,3-dione are prepared in the following 00 CO manner: Constituents (for 1000 tablets) ci 2-[1-(3-cyctopentyloxy-4i 1 methoxyphenyl)-2-(1,3,4o C methylisoindoline-1,3-dione..... 50.0 g In Slactose 50.7 g Cwheat 7.5 g polyethylene glycol 5.0 g 5.0 g magnesium 1.8 g demineralized water q.s.

The solid ingredients are first forced through a sieve of 0.6 mm mesh width. The active ingredient, lactose, talc, magnesium stearate and half of the starch then are mixed. The other half of the starch is suspended in 40 mL of water and this suspension is added to a boiling solution of the polyethylene glycol in 100 mL of water. The resulting paste is added to the pulverulent substances and the mixture is granulated, if necessary with the addition of water. The granulate is dried overnight at 35C, forced through a sieve of 1.2 mm mesh width and compressed to form tablets of approximately 6 mm diameter which are concave on both sides.

o Example Tablets, each containing 100 mg of 2-[1-(3-cyclopentyloxy-4-methoxyphenyl)-2- E (1,3,4-oxadiazol-2-yl)ethyl]-5-methylisoindoine-1,3-dione, can be prepared in the 0 0 following manner: Constituents (for 1000 tablets) Ci 2-[1-(3-cyclopentyloxy-4methoxyphenyl)-2-(1,3,4- N methylisoindoline-1,3-dione... 100.0 g 100.0 g N wheat 47.0 g magnesium 3.0 g All the solid ingredients are first forced through a sieve of 0.6 mm mesh width. The active ingredient, lactose, magnesium stearate and half of the starch then are mixed.

The other half of the starch is suspended in 40 mL of water and this suspension is added to 100 mL of boiling water. The resulting paste is added to the pulverulent substances and the mixture is granulated, if necessary with the addition of water. The granulate is dried overnight at 35*C, forced through a sieve of 1.2 mm mesh width and compressed to form tablets of approximately 6 mm diameter which are concave on both sides.

o Example 16 Tablets for chewing, each containing 75 mg of 2-[1-(3-cyclopentyloxy-4methoxyphenyl)- 2 -(1,3,4-oxadiazol-2-yl)ethyl]-5-methylisoindoline-1,3-dione, can be 00 prepared in the following manner: Composition (for 1000 tablets) 2-[1 -(3-cyclopentyloxy- 4 C methoxyphenyl)- 2 o N methylisoindoline-1,3-dione.... 75.0 g Smannitol 230.0 g lactose 150.0 g 2 1.0 g glycine 12.5 g stearic acid 10.0 g 1.g5 g gelatin solution q.s.

All the solid ingredients are first forced through a sieve of 0.25 mm mesh width.

The mannitol and the lactose are mixed, granulated with the addition of gelatin solution, forced through a sieve of 2 mm mesh width, dried at 50'C and again forced through a sieve of 1.7 mm mesh width. 2-[1-(3-Cyclopentyloxy-4-methoxyphenyl)-2-(1,3,4oxadiazol-2-yl)ethyl]-5-methylisoindoline-1,3-dione, the glycine and the saccharin are carefully mixed, the mannitol, the lactose granulate, the stearic acid and the talc are added and the whole is mixed thoroughly and compressed to form tablets of approximately 10 mm diameter which are concave on both sides and have a breaking groove on the upper side.

0 Example 17 0

N

Tablets, each containing 10 mg 2-[1-(3-cyclopentyloxy-4-methoxyphenyl)-2-(1,3,4- C oxadiazol-2-yl)ethyl]-5-methylisoindoline-1,3-dione, can be prepared in the following 00 manner: Composition (for 1000 tablets) N 2-[1-(3-cyclopentyloxy-4- Smethoxyphenyl)-2-(1,3,4o methylisoindoline-1,3-dione.... 10.0 g 328.5 g Scorn 17.5 g polyethylene glycol 6000 5.0 g talc.... 25.0 g magnesium 4.0 g demineralized water q.s.

The solid ingredients are first forced through a sieve of 0.6 mm mesh width. Then the active imide ingredient, lactose, talc, magnesium stearate and half of the starch are intimately mixed. The other half of the starch is suspended in 65 mL of water and this suspension is added to a boiling solution of the polyethylene glycol in 260 mL of water. The resulting paste is added to the pulverulent substances, and the whole is mixed and granulated, if necessary with the addition of water. The granulate is dried overnight at 0 C, forced through a sieve of 1.2 mm mesh width and compressed to form tablets of approximately 10 mm diameter which are concave on both sides and have a breaking notch on the upper side.

O

O Example 18 Ct Gelatin dry-filled capsules, each containing 100 mg of 2-[1-(3-cyclopentyloxy-4- 0 methoxyphenyl)- 2 -(l,3,4-oxadiazol-2-yl)ethyl]-5-methylisoindoline-1,3-dione, can be 00 prepared in the following manner: Composition (for 1000 capsules)

N

2-f1-(3-cyclopentyloxy- 4 CN methoxyphenyl)- 2 o N methylisoindoline-1,3-dione... 100.0 g o microcrystalline cellulose........ 30.0 g sodium lauryl 2.0 g magnesium 8.0 g The sodium lauryl sulfate is sieved into the 2-[1-(3-cyclopentyloxy- 4 methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-5-methylisoindoline-1,3-dione through a sieve of 0.2. mm mesh width and the two components are intimately mixed for minutes. The microcrystalline cellulose is then added through a sieve of 0.9 mm mesh width and the whole is again intimately mixed for 10 minutes. Finally, the magnesium stearate is added through a sieve of 0.8 mm width and, after mixing for a further 3 minutes, the mixture is introduced in portions of 140 mg each into size 0 (elongated) gelatin dry-fill capsules.

EXAMPLE 19 SGelatin dry-filled capsules, each containing 100 mg of 2-[1-(3-cyclopentyloxyo 4-methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-5-methylisoindoline-1,3-dione, can be prepared in the following manner: Composition (for 1000 capsules) o 2-[1 -(3-cyclopentyloxy-4methoxyphenyl)-2-(1,3,4oxadiazol-2-yl) Ci 5.0 g N 10 microcrystalline cellulose........ .30.0 g C sodium lauryl 2.0 g O magnesium 8.0 g The sodium lauryl sulfate is sieved into the 2-[1-(3-cyclopentyloxy-4methoxyphenyl)-2-(1,3,4-oxadiazol-2-yl)ethyl]-5-methylisoindoline-1,3-dione through a sieve of 0.2 mm mesh width and the two components are intimately mixed for 10 minutes. The microcrystalline cellulose is then added through a sieve of 0.9 mm mesh width and the whole is again intimately mixed for minutes. Finally, the magnesium stearate is added through a sieve of 0.8 mm width and, after mixing for a further 3 minutes, the mixture is introduced in portions of 140 mg each into size 0 (elongated) gelatin dry-fill capsules.

In the specification and claims the term "comprising" shall be understood to have a broad meaning similar to the term "including" and will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps. This definition also applies to variations on the term "comprising" such as "comprise" and "comprises".

Claims (27)

1. A method for the treatment or prophylaxis in a mammal of a condition selected from the group consisting of atopic dermatitis, psoriasis, lupus or viral infection, wherein the method comprises administering to the mammal an effective amount of a 1,3,4-oxadiazole compound selected from the group e consisting of a compound of the formula: SR O R' S0 R6 R2 N -N R3/ Y X R 4 in which: the carbon atom designated constitutes a center of chirality; Y is C=O, CH 2 SO2 or CH 2 C=O; X is hydrogen, or alkyl of 1 to 4 carbon atoms; each of R 1 R 2 R 3 and R 4 independently of the others, is hydrogen, halo, trifluoromethyl, acetyl, alkyl of 1 to 8 carbon atoms, alkoxy of 1 to 4 carbon atoms, nitro, cyano, hydroxy, tert-butyl,-CH 2 NRR 9 (CH 2 2 NRR 9 or-NR 8 R 9 or any two of R 1 R 2 R 3 and R 4 on adjacent carbon atoms, together with the depicted phenylene ring are naphthylidene, quinoline, quinoxaline, benzimidazole, benzodioxole or 2- hydroxybenzimidazole; each of R 5 and R 6 independently of the other, is hydrogen, alkyl of 1 to 4 carbon atoms, alkoxy of 1 to 6 carbon atoms, cyano, benzocycloalkoxy, cycloalkoxy of up to 18 carbon atoms, bicyloalkoxy of up to 18 carbon atoms, tricylcoalkoxy of up to 18 carbon atoms, or cycloalkylalkoxy of up to 18 carbon atoms; 00 O R 8 and R 9 taken together are tetramethylene, pentamethylene, -CHNCHCH-, Shexamethylene, or -CH 2 CH 2 X'CH 2 CH 2 Sin which X 1 is or -NH-; R 10 is hydrogen, alkyl of 1 to 8 carbon atoms, cycloalkyl, cycloalkylmethyl of up to 6 carbon atoms, phenyl, pyridyl, benzyl, imidazolylmethyl, pyridylmethyl, c- CH2NR14R15, or NR 11 R 12 c- wherein R 14 and R 15 independently of each other, are hydrogen, methyl, ethyl, or C propyl, and Swherein R 1 and R 12 independently of each other, are hydrogen, alkyl of 1 to 8 c 10 carbon atoms, phenyl, or benzyl; and the acid addition salts of said compounds which contain a nitrogen atom susceptible of protonation.

2. The method according to claim 1, wherein the viral infection is associated with the herpes virus or viral conjunctivitis.

3. The method according to claim 1, wherein the mammal is a non-human mammal and the viral infection is selected from the group consisting of feline immunodeficiency virus, equine infectious anaemia virus, caprine arthritis virus, visna virus and maedi virus.

4. The method according to any one of claims 1 to 3, wherein Y is C=0. The method according to any one of claims 1 to 3, wherein Y is CH 2

6. The method according to any one of claims 1 to 3, wherein each of R 2 R 3 and R 4 independently of the others, is hydrogen, halo, methyl, ethyl, methoxy, ethoxy, nitro, cyano, hydroxy, or -NR 8 R 9 in which each of R 8 and R 9 taken independently of the other is hydrogen, methyl or alkyl of 1 to 4 carbon atoms, or (ii) one of R 8 and R 9 is hydrogen and the other is -COCH 3 or (iii) one of R 8 and R 9 is hydrogen and the other is -CONH 2 or 00 41 O S(iv) one of R 8 and R 9 is hydrogen and the other is -COCH 2 NH 2 or SCOCH 2 N(CH 3 2 ct

7. The method according to any one of claims 1 to 6, wherein one of R 2 R 3 and R 4 is -NH 2 and the remaining of R 1 R 2 R 3 and R 4 are hydrogen. S8. The method according to any one of claims 1 to 6, wherein one of R 2 R 3 and R 4 is -NHCOCH 3 NHSO 2 R O or NHCOR 1 0 and the remaining of R 1 R 2 0R 3 and R 4 are hydrogen. N

9. The method according to any one of claims 1 to 6, wherein one of R 1 R 2 R 3 and R 4 is methyl or ethyl and the remaining of R 1 R 2 R 3 and R 4 are hydrogen.

10. The method according to any one of claims 1 to 6, wherein one of R 1 R 2 R 3 and R 4 is -N(CH 3 2 or hydroxy and the remaining of R 1 R 2 R 3 and R 4 are hydrogen.

11. The method according to any one of claims 1 to 3, wherein Y is C=O, X is hydrogen, and R 3 and R 4 taken together, are benzo.

12. The method compound according to any one of claims 1 to 3, wherein Y is C=O, X is hydrogen, and R 3 and R 4 taken together, are methylenedioxy.

13. The method according to any one of claims 1 to 12, wherein each of R and R 6 independently of the other, is methoxy, ethoxy, propoxy, isopropoxy, cyclopentoxy, cyclohexoxy, or bicycloalkoxy.

14. The method according to any one of claims 1 to 13, wherein R 5 is alkoxy and R 6 is alkoxy, cycloalkoxy, or bicycloalkoxy. The method according to any one of claims 1 to 14, wherein R 5 is methoxy and R 6 is methoxy, ethoxy, or cyclopentoxy.

16. The method according to any one of claims 1 to 12, wherein R 5 is methoxy and R 6 is bicycloalkoxy or benzoalkoxy.

17. The method according to any one of claims 1 to 16, wherein said compound is selected from the group consisting of a substantially chirally pure (R)-isomer, a substantially chirally pure (S)-isomer, or a mixture thereof, wherein the isomer is 2-[1 -(3-ethoxy-4-methoxyphenyl)-2-(1 ,3,4-oxadiazol-2- yl)ethyl]isoindoline-1,3-dione, 2-[1 -(3-ethoxy-4-methoxyphenyl)-2-( 1,3,4- oxadiazol-2-yl)ethyl]benzo[e]isoindoline-1 ,3-dione, 2-Il -(3-ethoxy-4-methoxy- phenyl)-2-( 1, 3,4-oxad iazol1-2-yl)ethyl]-4-m ethyl iso indoIi ne-1, ,3-d ione, 2-[1 ethoxy-4-m eth oxyp henyl)-2-(1 ,3,4-oxad iazol-2-yl)ethyl]-5-m ethyl iso ind olIine- 1, 3-dione, 2-[1 -(3-cyclopentyloxy-4-methoxyphenyl)-2-(1 ,3,4-oxadiazol-2- isoi ndo Ii ne- 1, 3-d ion e, 2-[1 -(3-cyclopentyloxy-4- methoxyp he nyl)-2-( 1, 3,4-oxad iazol-2-yl)ethyl]-4-m ethyl isoi nd olIine-1, ,3-d ion e, N-[2-[lI-(3-cyclopentlyoxy-4-methoxyphenyl)-2-( 1,3 ,4-oxadiazol-2-yl)ethyl]-1, 3- dioxoisoindolin-4-yl]acetamide, -(3-ethoxy-4-methoxyphenyl)-2-( 1,3,4- oxad iazol-2-yl)ethyl]-1 ,3-dioxoisoindolin-4-yl]acetamide, 5-(tert-butyl)-2-[ 1-(3- ethoxy-4-methoxyphenyl)-2-(1 ,3,4-oxad iazol-2-yl)ethyl]isoindoline-1 ,3-d ione, 2-[1 imethoxyphenyl)-2-(1I,3,4-oxad iazol-2-yl)ethyllisoindoline-1 ,3-dione, 2-[1 -(3-ethoxy-4-methoxyphenyl)-2-(1 ,3,4-oxadiazol-2-yl)ethyl]isoindolin- 1- one, 2-[1 -(3-ethoxy-4-methoxyphenyl)-2-(5-methyl( 1, 3,4-oxad iazol-2- yl))ethyl]isoindolin-1 -one, and 2-[1 -(3-ethoxy-4-methoxyphenyl)-2-(1 ,3,4- oxad iazol-2-yl)ethyl]-3-pyrrolino[3 ,4-]quinoline-1 ,3-dione.

18. The method according to any one of claims 1 to 6 or any one of claims 11 to 17, wherein two of R 1 R 2 and R 4 are hydrogen and the other two of R', R 2 R 3 and R 4 are not hydrogen.

19. The method according to any one of claims 1 to 6 or any one of claims 11 to 17, wherein two of R 1 R 2 R 3 and R 4 independent of each other, are alkyl of 1 to 8 carbon atoms, or alkoxy of 1 to 8 carbon atoms. 00 43 O 0 20. The method according to any one of claims 1 to 5 or any one of claims 11 Sto 19, wherein: Y is C=O or CH 2 each of R 2 R 3 and R 4 independently of the other, is hydrogen, halo, trifluoromethyl, acetyl, alkyl of 1 to 8 carbon atoms, alkoxy of 1 to 4 carbon Satoms, nitro, cyano, hydroxy, tert-butyl or -NR 8 R 9 and any two of R 1 R 2 R 3 and R 4 on adjacent carbon atoms, together with the Cdepicted phenylene ring are quinoline, quioxaline, 2-R 1 3 -benzimidazole, Sbenzodioxole or 2-hydroxybenzimidazole, c- 10 wherein R 13 is alkyl of 1 to 10 carbon atoms, -NH 2 or hydrogen.

21. The method according to any one of claims 1 to 3, wherein the compound is a substantially chirally pure (S)-isomer, a substantially chirally pure (R)-isomer or a mixture of and (S)-isomers and wherein: one of R 1 R 2 R 3 or R 4 is (CH 2 )nNR R 9 n is 0-2 and the other of R 2 R 3 or R 4 are hydrogen, halo, trifluoromethyl, acetyl, alkyl of 1 to 8 carbon atoms, alkoxy of 1 to 4 carbon atoms, nitro, cyano, hydroxy, tert-butyl; R 8 is hydrogen; R 9 is -COR 10 and, R 1 0 is -CH-(CH 3 2 cycloalkyl of 3 to 6 carbon atoms.

22. The method according to claim 21 wherein: one of R 1 R 2 R 3 or R 4 is (CH 2 )nNR 8 R 9 n is 0-2 and the other of R 1 R 2 R 3 or R 4 are hydrogen, R 8 is hydrogen; R 9 is -COR 10 and, R 10 is -CH-(CH 3 2

23. The method according to claim 21 wherein: one of R 1 R 2 R 3 or R 4 is (CH 2 )nNR'R 9 n is 0-2 and the other of R 1 R 2 R 3 or R 4 are hydrogen, R 8 is hydrogen; 00 44 O R 9 is -CORO; and, R 10 is cycloalkyl of 3 to 6 carbon atoms.

24. The method according to claim 23 where R 1 0 is cyclopropyl. The method according to claim 21 wherein: one of R 1 R 2 R 3 or R 4 is (CH 2 )nNR'R 9 n is 0-2 and the other of R 2 R 3 or R 4 are hydrogen, SR 8 is hydrogen; c 10 R 9 is -COR 10 and, R 10 is CH 2 -C-C 3 H 5

26. The method of any one of claims 1 to 3, wherein the 1,3,4-oxadiazole compound is substantially as herein described with reference to the examples.

27. Use of a 1,3,4-oxadiazole compound selected from the group consisting of a compound of the formula: R NN -N R3A vX R3 Y I X 0 X R 4 in which: the carbon atom designated constitutes a centre of chirality; Y is C=O, CH 2 ,S0 2 or CH 2 C=O; X is hydrogen, or alkyl of 1 to 4 carbon atoms; each of R 1 R 2 R 3 and R 4 independently of the others, is hydrogen, halo, trifluoromethyl, acetyl, alkyl of 1 to 8 carbon atoms, alkoxy of 1 to 4 carbon atoms, nitro, cyano, hydroxy, tert-butyl,-CH 2 NR 8 R 9 (CH 2 2 NR'R 9 or-NRR 9 or any two of R 1 R 2 R 3 and R 4 on adjacent carbon atoms, together with the depicted phenylene ring are naphthylidene, quinoline, quinoxaline, benzimidazole, benzodioxole or 2- hydroxybenzimidazole; each of R 5 and R 6 independently of the other, is hydrogen, alkyl of 1 to 4 carbon atoms, alkoxy of 1 to 6 carbon atoms, cyano, benzocycloalkoxy, cycloalkoxy of up to 18 carbon atoms, bicyloalkoxy of up to 18 carbon atoms, tricylcoalkoxy of up to 18 carbon atoms, or cycloalkylalkoxy of up to 18 carbon atoms; each of R 8 and R 9 taken independently of the other is hydrogen, straight alkyl of 1 to 8 carbon atoms, branched alkyl of 1 to 8 carbon atoms, phenyl, benzyl, pyridyl, pyridylmethyl, or one of R 8 and R 9 is hydrogen and the other is -COR 10 or -SO 2 R' 1 or R 8 and R 9 taken together are tetramethylene, pentamethylene, -CHNCHCH-, hexamethylene, or -CH 2 CH 2 X CH 2 CH 2 in which X 1 is or -NH-; R 10 is hydrogen, alkyl of 1 to 8 carbon atoms, cycloalkyl, cycloalkylmethyl of up to 6 carbon atoms, phenyl, pyridyl, benzyl, imidazolylmethyl, pyridylmethyl, CH 2 NR 1 4 R' 1 or NR 1 R 12 wherein R 1 4 and R 1 i independently of each other, are hydrogen, methyl, ethyl, or propyl, and wherein R" and R 1 2 independently of each other, are hydrogen, alkyl of 1 to 8 carbon atoms, phenyl, or benzyl; and the acid addition salts of said compounds which contain a nitrogen atom susceptible of protonation in the preparation of a medicament for the treatment or prophylaxis in a mammal of a condition selected from the group consisting of atopic dermatitis, psoriasis, lupus or viral infection.

28. The use according to claim 27 wherein the viral infection is associated with the herpes virus or viral conjunctivitis. 00 46 O 29. The use according to claim 27, wherein the mammal is a non-human mammal and the viral infection is selected from the group consisting of feline Simmunodeficiency virus, equine infectious anaemia virus, caprine arthritis virus, visna virus and maedi virus. Use of a 1,3,4-oxadiazole compound selected from the group N consisting of c a compound of the formula: (i RR pR 6 R2 N-N X R 4 in which: the carbon atom designated constitutes a centre of chirality; Y is C=O, CH 2 ,S0 2 or CH 2 C=O; X is hydrogen, or alkyl of 1 to 4 carbon atoms; each of R 2 R 3 and R 4 independently of the others, is hydrogen, halo, trifluoromethyl, acetyl, alkyl of 1 to 8 carbon atoms, alkoxy of 1 to 4 carbon atoms, nitro, cyano, hydroxy, tert-butyl,-CH 2 NR 8 R 9 (CH 2 2 NRsR 9 or-NR 8 R 9 or any two of R 1 R 2 R 3 and R 4 on adjacent carbon atoms, together with the depicted phenylene ring are naphthylidene, quinoline, quinoxaline, benzimidazole, benzodioxole or 2- hydroxybenzimidazole; each of R 5 and R 6 independently of the other, is hydrogen, alkyl of 1 to 4 carbon atoms, alkoxy of 1 to 6 carbon atoms, cyano, benzocycloalkoxy, cycloalkoxy of up to 18 carbon atoms, bicyloalkoxy of up to 18 carbon atoms, tricylcoalkoxy of up to 18 carbon atoms, or cycloalkylalkoxy of up to 18 carbon 00 47 O O atoms; each of R 8 and R 9 taken independently of the other is hydrogen, straight alkyl of 1 to 8 carbon atoms, branched alkyl of 1 to 8 carbon atoms, phenyl, benzyl, pyridyl, pyridylmethyl, or one of R 8 and R 9 is hydrogen and the other is -COR 10 or -S02RO, or R 8 and R 9 taken together are tetramethylene, pentamethylene, -CHNCHCH-, Shexamethylene, or -CH 2 CH 2 X'CH 2 CH 2 Sin which X 1 is or -NH-; SR' 0 is hydrogen, alkyl of 1 to 8 carbon atoms, cycloalkyl, cycloalkylmethyl of up to 6 carbon atoms, phenyl, pyridyl, benzyl, imidazolylmethyl, pyridylmethyl, c 10 CH 2 NR 14 R i s or NR'R 12 wherein R 14 and R 15 independently of each other, are hydrogen, methyl, ethyl, or propyl, and wherein R" and R 12 independently of each other, are hydrogen, alkyl of 1 to 8 carbon atoms, phenyl, or benzyl; and the acid addition salts of said compounds which contain a nitrogen atom susceptible of protonation and the compound is a substantially chirally pure (R)-isomer, a substantially chirally pure (S)-isomer, or a mixture thereof, in the preparation of a medicament for reducing or inhibiting undesirable levels of TNFa in a mammal.

31. Use of a 1,3,4-oxadiazole compound selected from the group consisting of a compound of the formula: R R' 1 N N R3/ Y O X R 4 in which: the carbon atom designated constitutes a centre of chirality; 00 48 O Y is C=O, CH 2 ,S0 2 or CH 2 C=O; a X is hydrogen, or alkyl of 1 to 4 carbon atoms; each of R 2 R 3 and R 4 independently of the others, is hydrogen, halo, trifluoromethyl, acetyl, alkyl of 1 to 8 carbon atoms, alkoxy of 1 to S4 carbon atoms, nitro, cyano, hydroxy, tert-butyl,-CH 2 NRR 9 (CH 2 2 NRR 9 or-NR 8 R 9 C- or any two of R 2 R 3 and R 4 on adjacent carbon atoms, together with the Sdepicted phenylene ring are naphthylidene, quinoline, c 10 quinoxaline, benzimidazole, benzodioxole or 2- hydroxybenzimidazole; each of R 5 and R 6 independently of the other, is hydrogen, alkyl of 1 to 4 carbon atoms, alkoxy of 1 to 6 carbon atoms, cyano, benzocycloalkoxy, cycloalkoxy of up to 18 carbon atoms, bicyloalkoxy of up to 18 carbon atoms, tricylcoalkoxy of up to 18 carbon atoms, or cycloalkylalkoxy of up to 18 carbon atoms; each of R 8 and R 9 taken independently of the other is hydrogen, straight alkyl of 1 to 8 carbon atoms, branched alkyl of 1 to 8 carbon atoms, phenyl, benzyl, pyridyl, pyridylmethyl, or one of R 8 and R 9 is hydrogen and the other is -COR 10 or -S0 2 R 1 or R 8 and R 9 taken together are tetramethylene, pentamethylene, -CHNCHCH-, hexamethylene, or -CH 2 CH 2 XCH 2 CH 2 in which X 1 is or -NH-; R 10 is hydrogen, alkyl of 1 to 8 carbon atoms, cycloalkyl, cycloalkylmethyl of up to 6 carbon atoms, phenyl, pyridyl, benzyl, imidazolylmethyl, pyridylmethyl, CH 2 NR 1 4 R 15 or NR 1 R 12 wherein R 14 and R 15 independently of each other, are hydrogen, methyl, ethyl, or propyl, and wherein R 1 and R 12 independently of each other, are hydrogen, alkyl of 1 to 8 carbon atoms, phenyl, or benzyl; and the acid addition salts of said compounds which contain a nitrogen atom susceptible of protonation and 00 49 O O the compound is a substantially chirally pure (R)-isomer, a substantially chirally 0 pure (S)-isomer, or a mixture thereof, for the preparation of a medicament for inhibiting phosphodiesterase type 4 in a mammal.

32. Use of a 1,3,4-oxadiazole compound selected from the group consisting of a compound of the formula: SR6 SY N R 3 Y N O X R 4 in which: the carbon atom designated constitutes a centre of chirality; Y is C=0, CH2,SO2 or X is hydrogen, or alkyl of 1 to 4 carbon atoms; each of R 1 R 2 R 3 and R 4 independently of the others, is hydrogen, halo, trifluoromethyl, acetyl, alkyl of 1 to 8 carbon atoms, alkoxy of 1 to 4 carbon atoms, nitro, cyano, hydroxy, tert-butyl,-CH 2 NR 8 R 9 (CH 2 2 NR'R 9 or-NR 8 R; or any two of R 1 R 2 R 3 and R 4 on adjacent carbon atoms, together with the depicted phenylene ring are naphthylidene, quinoline, quinoxaline, benzimidazole, benzodioxole or 2- hydroxybenzimidazole; each of R 5 and R 6 independently of the other, is hydrogen, alkyl of 1 to 4 carbon atoms, alkoxy of 1 to 6 carbon atoms, cyano, benzocycloalkoxy, cycloalkoxy of up to 18 carbon atoms, bicyloalkoxy of up to 18 carbon atoms, tricylcoalkoxy of up to 18 carbon atoms, or cycloalkylalkoxy of up to 18 carbon atoms; each of R 8 and R 9 taken independently of the other is hydrogen, 00 O 0 straight alkyl of 1 to 8 carbon atoms, branched alkyl of 1 to 8 carbon atoms, phenyl, n benzyl, pyridyl, pyridylmethyl, or one of R 8 and R 9 is hydrogen and the other is F COR 10 or -SO2R or R 8 and R 9 taken together are tetramethylene, pentamethylene, -CHNCHCH-, hexamethylene, or -CH 2 CH 2 XlCH 2 CH 2 IN in which X 1 is or -NH-; R 1 is hydrogen, alkyl of 1 to 8 carbon atoms, cycloalkyl, cycloalkylmethyl of up to 6 carbon atoms, phenyl, pyridyl, benzyl, imidazolylmethyl, pyridylmethyl, CH 2 NR 1 4 R 5 or SNR"R 12 wherein R 1 4 and R 1 5 independently of each other, are hydrogen, methyl, ethyl, or propyl, and wherein R 1 and R 12 independently of each other, are hydrogen, alkyl of 1 to 8 carbon atoms, phenyl, or benzyl; and the acid addition salts of said compounds which contain a nitrogen atom susceptible of protonation and the compound is a substantially chirally pure (R)-isomer, a substantially chirally pure (S)-isomer, or a mixture thereof, for preparation of a medicament for treating in a mammal a disease selected from the group consisting of inflammatory bowel diseases and, autoimmune diseases, selected from, but not limited to the group consisting of arthritis, rheumatoid arthritis, inflammatory bowel disease, Crohn's disease, aphthous ulcers, cachexia, multiple sclerosis, graft versus host disease, asthma, adult respiratory distress syndrome, and acquired immune deficiency syndrome.

33. Use of a 1,3,4-oxadiazole compound selected from the group consisting of a compound of the formula: 00 51 O R SR 6 R 2 N-N N R 3 Y- c X r R 4 Sin which: CN the carbon atom designated constitutes a centre of chirality; Y is C=O, CH 2 ,SO 2 or CH 2 C=O; X is hydrogen, or alkyl of 1 to 4 carbon atoms; each of R 1 R 2 R 3 and R 4 independently of the others, is hydrogen, halo, trifluoromethyl, acetyl, alkyl of 1 to 8 carbon atoms, alkoxy of 1 to 4 carbon atoms, nitro, cyano, hydroxy, tert-butyl,-CH 2 NRR 9 (CH 2 2 NR'R 9 or-NR 8 R 9 or any two of R 1 R 2 R 3 and R 4 on adjacent carbon atoms, together with the depicted phenylene ring are naphthylidene, quinoline, quinoxaline, benzimidazole, benzodioxole or 2- hydroxybenzimidazole; each of R 5 and R 6 independently of the other, is hydrogen, alkyl of 1 to 4 carbon atoms, alkoxy of 1 to 6 carbon atoms, cyano, benzocycloalkoxy, cycloalkoxy of up to 18 carbon atoms, bicyloalkoxy of up to 18 carbon atoms, tricylcoalkoxy of up to 18 carbon atoms, or cycloalkylalkoxy of up to 18 carbon atoms; each of R 8 and R 9 taken independently of the other is hydrogen, straight alkyl of 1 to 8 carbon atoms, branched alkyl of 1 to 8 carbon atoms, phenyl, benzyl, pyridyl, pyridylmethyl, or one of R 8 and R 9 is hydrogen and the other is -COR 1 0 or -S0 2 R 1 0 or R 8 and R 9 taken together are tetramethylene, pentamethylene, -CHNCHCH-, hexamethylene, or -CH 2 CH 2 X'CH 2 CH 2 in which X 1 is or -NH-; 00 52 O 0 R 1 0 is hydrogen, alkyl of 1 to 8 carbon atoms, cycloalkyl, cycloalkylmethyl of up to 6 carbon atoms, phenyl, pyridyl, benzyl, imidazolylmethyl, pyridylmethyl,, SCH 2 NR 14 R' i or NR 1 "R 12 wherein R 1 4 and R 15 independently of each other, are hydrogen, methyl, ethyl, or propyl, and wherein R 1 and R 12 independently of each other, are hydrogen, alkyl of 1 to 8 C carbon atoms, phenyl, or benzyl; and S(b) the acid addition salts of said compounds which contain a nitrogen atom Ssusceptible of protonation and c 10 the compound is a substantially chirally pure (R)-isomer, a substantially chirally pure (S)-isomer, or a mixture thereof for the preparation of a medicament for treating cancer in a mammal.

34. A Use of a 1,3,4-oxadiazole compound selected from the group consisting of a compound of the formula: R R 6 R 0 X N N R3 Y 0O X R 4 in which: the carbon atom designated constitutes a centre of chirality; Y is C=O, CH 2 ,S0 2 or CH 2 C=O X is hydrogen, or alkyl of 1 to 4 carbon atoms; each of R 1 R 2 R 3 and R 4 independently of the others, is hydrogen, halo, trifluoromethyl, acetyl, alkyl of 1 to 8 carbon atoms, alkoxy of 1 to 4 carbon atoms, nitro, cyano, hydroxy, tert-butyl,-CH 2 NR 8 R 9 (CH 2 2 NR 8 R 9 or-NR 8 R 9 or any two of R 1 R 2 R 3 and R 4 on adjacent carbon atoms, together with the depicted phenylene ring are naphthylidene, quinoline, quinoxaline, benzimidazole, benzodioxole or 2- hydroxybenzimidazole; each of R 5 and R 6 independently of the other, is hydrogen, alkyl of 1 to 4 carbon atoms, alkoxy of 1 to 6 carbon atoms, cyano, benzocycloalkoxy, cycloalkoxy of up to 18 carbon atoms, bicyloalkoxy of up to 18 carbon atoms, tricylcoalkoxy of up to 18 carbon atoms, or cycloalkylalkoxy of up to 18 carbon atoms; each of R 8 and R 9 taken independently of the other is hydrogen, straight alkyl of 1 to 8 carbon atoms, branched alkyl of 1 to 8 carbon atoms, phenyl, benzyl, pyridyl, pyridylmethyl, or one of R 8 and R 9 is hydrogen and the other is -COR 10 or -SO 2 R' 0 or R 8 and R 9 taken together are tetramethylene, pentamethylene, -CHNCHCH-, hexamethylene, or -CH 2 CH 2 X'CH 2 CH 2 in which X' is or -NH-; R 10 is hydrogen, alkyl of 1 to 8 carbon atoms, cycloalkyl, cycloalkylmethyl of up to 6 carbon atoms, phenyl, pyridyl, benzyl, imidazolylmethyl, pyridylmethyl, CH 2 NR 14 R 15 or NR 11 R 12 wherein R 14 and R s 1 independently of each other, are hydrogen, methyl, ethyl, or propyl, and wherein R" and R 1 2 independently of each other, are hydrogen, alkyl of 1 to 8 carbon atoms, phenyl, or benzyl; and the acid addition salts of said compounds which contain a nitrogen atom susceptible of protonation and the compound is a substantially chirally pure (R)-isomer, a substantially chirally pure (S)-isomer, or a mixture thereof, for the preparation of a medicament for treating undesirable angiogenesis in a mammal. Use of a 1,3,4-oxadiazole compound selected from the group consisting of a compound of the formula 00 54 O O R SR 6 cI0 -R6 R 2 I-N N N R 3 Y I 0 x tr R 4 Sin which: c-I the carbon atom designated constitutes a centre of chirality; Y is C=O, CH 2 ,S0 2 or CH 2 C=O X is hydrogen, or alkyl of 1 to 4 carbon atoms; each of R 2 R 3 and R 4 independently of the others, is hydrogen, halo, trifluoromethyl, acetyl, alkyl of 1 to 8 carbon atoms, alkoxy of 1 to 4 carbon atoms, nitro, cyano, hydroxy, tert-butyl,-CH 2 NRR, (CH 2 2 NRSR 9 or-NR 8 R 9 or any two of R 1 R 2 R 3 and R 4 on adjacent carbon atoms, together with the depicted phenylene ring are naphthylidene, quinoline, quinoxaline, benzimidazole, benzodioxole or 2- hydroxybenzimidazole; each of R 5 and R 6 independently of the other, is hydrogen, alkyl of 1 to 4 carbon atoms, alkoxy of 1 to 6 carbon atoms, cyano, benzocycloalkoxy, cycloalkoxy of up to 18 carbon atoms, bicyloalkoxy of up to 18 carbon atoms, tricylcoalkoxy of up to 18 carbon atoms, or cycloalkylalkoxy of up to 18 carbon atoms; each of R 8 and R 9 taken independently of the other is hydrogen, straight alkyl of 1 to 8 carbon atoms, branched alkyl of 1 to 8 carbon atoms, phenyl, benzyl, pyridyl, pyridylmethyl, or one of R 8 and R 9 is hydrogen and the other is -COR' 1 or -S0 2 R 1 0 or R 8 and R 9 taken together are tetramethylene, pentamethylene, -CHNCHCH-, hexamethylene, or -CH 2 CH 2 X'CH 2 CH 2 in which X 1 is or -NH-; 00 O R 10 is hydrogen, alkyl of 1 to 8 carbon atoms, cycloalkyl, cycloalkylmethyl of up to 6 carbon atoms, phenyl, pyridyl, benzyl, imidazolylmethyl, pyridylmethyl, CH 2 NR 1 4 R 1 5 or NR"R 12 wherein R 1 4 and R 1 5 independently of each other, are hydrogen, methyl, ethyl, or propyl, and wherein R 11 and R 1 2 independently of each other, are hydrogen, alkyl of 1 to 8 C, carbon atoms, phenyl, or benzyl; and (c the acid addition salts of said compounds which contain a nitrogen atom Ssusceptible of protonation and c 10 the compound is a substantially chirally pure (R)-isomer, a substantially chirally pure (S)-isomer, or a mixture thereof for the preparation of a medicament for treating inflammation in a mammal.