AU2004223394A1 - Glucosidases, nucleic acids encoding them and methods for making and using them - Google Patents
Glucosidases, nucleic acids encoding them and methods for making and using them Download PDFInfo
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- AU2004223394A1 AU2004223394A1 AU2004223394A AU2004223394A AU2004223394A1 AU 2004223394 A1 AU2004223394 A1 AU 2004223394A1 AU 2004223394 A AU2004223394 A AU 2004223394A AU 2004223394 A AU2004223394 A AU 2004223394A AU 2004223394 A1 AU2004223394 A1 AU 2004223394A1
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Classifications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/02—Nutrients, e.g. vitamins, minerals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C1/00—Preparation of malt
- C12C1/027—Germinating
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
- C12C5/004—Enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
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- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
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- Public Health (AREA)
- Obesity (AREA)
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- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- Diabetes (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
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|---|---|---|---|
| US45697203P | 2003-03-20 | 2003-03-20 | |
| US60/456,972 | 2003-03-20 | ||
| PCT/US2004/008541 WO2004085615A2 (en) | 2003-03-20 | 2004-03-18 | Glucosidases, nucleic acids encoding them and methods for making and using them |
Publications (1)
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| AU2004223394A1 true AU2004223394A1 (en) | 2004-10-07 |
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|---|---|---|---|
| AU2004223394A Abandoned AU2004223394A1 (en) | 2003-03-20 | 2004-03-18 | Glucosidases, nucleic acids encoding them and methods for making and using them |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20070056053A1 (https=) |
| EP (1) | EP1608766A4 (https=) |
| JP (1) | JP2006524050A (https=) |
| AU (1) | AU2004223394A1 (https=) |
| CA (1) | CA2519580A1 (https=) |
| WO (1) | WO2004085615A2 (https=) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20040005673A1 (en) * | 2001-06-29 | 2004-01-08 | Kevin Jarrell | System for manipulating nucleic acids |
| CA2360011A1 (en) | 1999-01-05 | 2000-07-13 | Trustees Of Boston University | Ordered gene assembly |
| US7435562B2 (en) * | 2000-07-21 | 2008-10-14 | Modular Genetics, Inc. | Modular vector systems |
| AU2001277014A1 (en) * | 2000-07-21 | 2002-02-05 | Trustees Of Boston University | Modular vector systems |
| US7024800B2 (en) | 2004-07-19 | 2006-04-11 | Earthrenew, Inc. | Process and system for drying and heat treating materials |
| US7685737B2 (en) | 2004-07-19 | 2010-03-30 | Earthrenew, Inc. | Process and system for drying and heat treating materials |
| WO2006053131A1 (en) * | 2004-11-11 | 2006-05-18 | Modular Genetics, Inc. | Ladder assembly and system for generating diversity |
| US7610692B2 (en) | 2006-01-18 | 2009-11-03 | Earthrenew, Inc. | Systems for prevention of HAP emissions and for efficient drying/dehydration processes |
| JP4964710B2 (ja) * | 2007-08-29 | 2012-07-04 | 群栄化学工業株式会社 | β−グルカン含有穀物糖化物の製造方法 |
| BRPI0822090A2 (pt) * | 2007-12-19 | 2017-05-23 | Novozymes As | polipeptídeo isolado, polinucleotídeo isolado, construção de ácido nucléico, célula hospedeira recombinante, métodos para produzir o polipeptídeo, para produzir um mutante de uma célula precursora, de inibir a expressão de um polipeptídeo, para produzir uma proteína, para degradar ou converter um material celulósico, para a produção de um produto de fermentação, para fermentar um material celulósico, planta transgênica, parte vegetal ou célula vegetal, molécula de rna inibidora de duplo filamento |
| CA2709485A1 (en) * | 2007-12-19 | 2009-07-09 | Novozymes A/S | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| US20100306881A1 (en) * | 2007-12-19 | 2010-12-02 | Novozymes A/S | Polypeptides having Cellulolytic Enhancing Activity and Polynucleotides Encoding Same |
| NZ598403A (en) * | 2007-12-19 | 2012-07-27 | Novozymes As | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| WO2012161250A1 (ja) * | 2011-05-24 | 2012-11-29 | 日本食品化工株式会社 | α-グルコシダーゼ、その製造方法およびその用途、並びにジンゲロール配糖体、その製造方法およびその用途 |
| AU2012340849B2 (en) | 2011-11-21 | 2017-11-02 | Innovation Hammer Llc | Methods and systems for growing plants using silicate-based substrates, cultivation of enhanced photosynthetic productivity and photosafening by utilization of exogenous glycopyranosides for endogenous glycopyranosyl-protein derivatives, and formulations, processes and systems for the same |
| WO2013176731A2 (en) | 2012-05-21 | 2013-11-28 | Nonomura Arthur M | Methods for rendering micellar coordination complexes safe for the treatment of plants and formulations for same |
| BR112018072235B1 (pt) | 2016-04-29 | 2023-01-17 | Innovation Hammer Llc | Método para intensificar o crescimento respiratório de um organismo fotossintético que compreende aplicar uma formulação de compósito de glicano |
| CN108796095B (zh) * | 2018-07-03 | 2021-11-26 | 中国水产科学研究院黑龙江水产研究所 | 一种提高鲤饲料转化效率的选育方法 |
| EP4077357B1 (en) * | 2019-12-17 | 2023-09-06 | Navigo Proteins Gmbh | Binding proteins for the enzyme acid alpha glucosidase (gaa) and uses thereof |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2615810A (en) * | 1948-03-04 | 1952-10-28 | Wallerstein Company | Retarding the staling of bakery products |
| US3026205A (en) * | 1959-08-19 | 1962-03-20 | Baxter Laboratories Inc | Process of producing baked confections and the products resulting therefrom by alpha amylase |
| US5021246A (en) * | 1984-03-30 | 1991-06-04 | Anheuser-Busch, Incorporated | Step mashing process for producing low alcohol beer |
| US4788066A (en) * | 1987-12-14 | 1988-11-29 | Grain Processing Corporation | Preparation of low alcohol beer |
| DK474589D0 (da) * | 1989-09-27 | 1989-09-27 | Novo Nordisk As | Fremgangsmaade til fremstilling af bageriprodukter |
| US6355467B1 (en) * | 1989-10-20 | 2002-03-12 | Johns Hopkins University | Saccharification enzymes from hyperthermophilic bacteria and processes for their production |
| US5326477A (en) * | 1990-05-07 | 1994-07-05 | Bio-Sep, Inc. | Process for digesting solid waste |
| NL9100050A (nl) * | 1991-01-11 | 1992-08-03 | Heineken Technische Beheer Bv | Werkwijze voor het continu bereiden van wort. |
| US5405624A (en) * | 1991-02-14 | 1995-04-11 | Bio-Technical Resources | Process for producing a product with an intensified beer flavor |
| BR9307805A (pt) * | 1992-12-31 | 1996-07-30 | Metallgesellschaft Ag | Processo para a preparaçao de cerveja |
| US5824531A (en) * | 1994-03-29 | 1998-10-20 | Novid Nordisk | Alkaline bacilus amylase |
| US5582681A (en) * | 1994-06-29 | 1996-12-10 | Kimberly-Clark Corporation | Production of soft paper products from old newspaper |
| US6093562A (en) * | 1996-02-05 | 2000-07-25 | Novo Nordisk A/S | Amylase variants |
| EP0839224A1 (en) * | 1995-07-19 | 1998-05-06 | Novo Nordisk A/S | Treatment of fabrics |
| US6197070B1 (en) * | 1996-05-15 | 2001-03-06 | The Procter & Gamble Company | Detergent compositions comprising alpha combination of α-amylases for malodor stripping |
| AU4067297A (en) * | 1996-08-16 | 1998-03-06 | International Paper Company | Enzymatic freeness enhancement |
| US6326341B1 (en) * | 1996-09-11 | 2001-12-04 | The Procter & Gamble Company | Low foaming automatic dishwashing compositions |
| AU4550997A (en) * | 1996-10-11 | 1998-05-11 | Novo Nordisk A/S | Cellulose binding domains (cbds) for oral care products |
| WO1998026058A1 (fr) * | 1996-12-11 | 1998-06-18 | Universite De Reims Champagne Ardennes | Alpha-glucosidase et pullulanase thermostables et leurs utilisations |
| US6069122A (en) * | 1997-06-16 | 2000-05-30 | The Procter & Gamble Company | Dishwashing detergent compositions containing organic diamines for improved grease cleaning, sudsing, low temperature stability and dissolution |
| DE19703364A1 (de) * | 1997-01-30 | 1998-08-06 | Henkel Ecolab Gmbh & Co Ohg | Pastenförmiges Wasch- und Reinigungsmittel |
| WO1998040413A1 (en) * | 1997-03-14 | 1998-09-17 | National Starch And Chemical Investment Holding Corporation | Isolation of hemicellulose from corn fiber |
| US6204232B1 (en) * | 1997-10-30 | 2001-03-20 | Novo Nordisk A/S | α-amlase mutants |
| EP1032644B1 (en) * | 1997-11-10 | 2003-03-19 | The Procter & Gamble Company | Process for preparing a detergent tablet |
| JP2003527450A (ja) * | 1998-05-01 | 2003-09-16 | ノボ ノルディスク アクティーゼルスカブ | N−ヒドロキシアセトアニリドの様な増強剤 |
| DE19824705A1 (de) * | 1998-06-03 | 1999-12-09 | Henkel Kgaa | Amylase und Protease enthaltende Wasch- und Reinigungsmittel |
| JP4335995B2 (ja) * | 1998-07-22 | 2009-09-30 | 昭 神谷 | 環境保全型粒状洗浄用組成物 |
| AU6046199A (en) * | 1998-09-17 | 2000-04-03 | Novozymes North America, Inc. | Methods for deinking and decolorizing printed paper |
| US6309871B1 (en) * | 1999-03-31 | 2001-10-30 | Novozymes A/S | Polypeptides having alkaline α-amylase activity |
| US7314974B2 (en) * | 2002-02-21 | 2008-01-01 | Monsanto Technology, Llc | Expression of microbial proteins in plants for production of plants with improved properties |
| US7741092B2 (en) * | 2002-10-31 | 2010-06-22 | Verenium Corporation | Amylases, nucleic acids encoding them and methods for making and using them |
-
2004
- 2004-03-18 JP JP2006507400A patent/JP2006524050A/ja not_active Abandoned
- 2004-03-18 WO PCT/US2004/008541 patent/WO2004085615A2/en not_active Ceased
- 2004-03-18 US US10/550,155 patent/US20070056053A1/en not_active Abandoned
- 2004-03-18 CA CA002519580A patent/CA2519580A1/en not_active Abandoned
- 2004-03-18 EP EP04757926A patent/EP1608766A4/en not_active Withdrawn
- 2004-03-18 AU AU2004223394A patent/AU2004223394A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004085615A2 (en) | 2004-10-07 |
| US20070056053A1 (en) | 2007-03-08 |
| EP1608766A4 (en) | 2006-11-02 |
| CA2519580A1 (en) | 2004-10-07 |
| WO2004085615A3 (en) | 2005-05-19 |
| JP2006524050A (ja) | 2006-10-26 |
| EP1608766A2 (en) | 2005-12-28 |
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