AU2004201386A1 - 5-heterocyclyl pyrazolo[4,3-d]pyrimidin-7-ones for the treatment of male erectile dysfunction - Google Patents

Description

-1-

AUSTRALIA

PATENTS ACT 1990 COMPLETE SPECIFICATION FOR A STANDARD PATENT

ORIGINAL

Name of Applicant/s: Ortho-McNeil Pharmaceutical, Inc.

Actual Inventor/s: Zhihua Sui and Jihua Guan and Mark J. Macielag Address for Service: Baldwin Shelston Waters MARGARET STREET SYDNEY NSW 2000 CCN: 3710000352 Invention Title: 5-HETEROCYCLYL PYRAZOLO[4,3-D]PYRIMIDIN-7-ONES FOR THE TREATMENT OF MALE ERECTILE DYSFUNCTION Details of Original Application No. 57041/99 dated 02 Sep 1999 The following statement is a full description of this invention, including the best method of performing it known to me/us:- File: 31027AUP01 500332931 1.DOC/5844 Title of the Invention PYRAZOLO[4,3-D]PYRIMIDIN-7-ONES FOR THE TREATMENT OF MALE ERECTILE DYSFUNCTION Cross-Reference to Related Application This application claims priority from United States provisional application Serial No. 60/099,268, filed September 4, 1998, the contents of which are hereby incorporated by reference.

Field of the Invention This invention relates to certain novel compounds, their synthesis and their use for the treatment of male erectile dysfunction. More particularly, the compounds of the present invention are substituted pyrazolopyrimidinones useful for the treatment of male erectile dysfunction.

Backaround of the Invention Erectile dysfunction (ED) is defined as the inability to achieve or maintain an erection sufficiently rigid for satisfactory sexual intercourse.

Currently it is estimated that approximately 7-8% of the male population suffer from some degree of ED, the equivalent of at least 20 million men in the United States alone. Since the likelihood of ED increases with age, it is projected that the incidence of this condition will rise in the future as the average age of the population increases.

Male erectile dysfunction may be the consequence of psychogenic and/or organic factors. Although ED is multi-factorial, certain sub-groups within the male population are more likely to present with the symptoms of the disorder. In particular, patients with diabetes, hypertension, heart disease, and multiple sclerosis have a particularly high prevalence of ED. In addifion, -lapatients who take certain classes of drugs such as antihypertensives, antidepressants, sedatives, and anxiolytics are more prone to suffer from ED.

Treatments for ED include a variety of pharmacologic agents, vacuum devices, and penile prostheses. Among the pharmacologic agents, papaverine, phentolamine, and alprostadil are currently used in practice.

These agents are only effective after direct intracavernosal or intraurethral injection, and are associated with side effects such as priapism, fibrosis, penile pain and hematoma at the injection site. Vacuum devices are a noninasive alternative treatment for ED. These devices produce an erection by creating a negative pressure around the shaft of the penis resulting in an increased blood flow into the corpus cavernosum via passive arterial dilation. Although this form of therapy is frequently successful in ED of organic origin, complaints include the lack of spontaneity and the time involved in using a mechanical device, and difficulty and discomfort with ejaculation. A variety of semi-rigid or inflatable penile prostheses have been used with some success, particularly in diabetic men. These devices are generally considered when other treatment options have failed, and are associated with an increased risk of infection and ischemia.

Recently, the phosphodiesterase V (PDEV) inhibitor, sildenafil (Viagra®) was approved by the FDA as an orally effective medication for the treatment of ED. Sildenafil, 5-[2-ethoxy-5-(4-methylpiperazin-1-ylsulphonyl)phenyl]-1methyl-3-n-propyl-6,7-dihydro-1H-pyrazolo[4,3-d]pyrimidin-7-one and a number of related analogs and their use as antianginal agents are described in U.S.

Patent Nos. 5,250,534 and 5,346,901. The use of sildenafil and related analogs for treating male erectile dysfuntion is described in PCT International Application Publication No. WO 94/28902, published December 22, 1994. In clinical studies, the drug improved sexual function in about 70% of the men who suffer from ED of psychogenic or organic etiology. However, the drug showed less dramatic efficacy in patients who had undergone a radical prostatectomy, with improved erections in 43% of patients who took sildenafil versus 15% on placebo. In addition, the use of sildenafil is associated with several undesirable side effects including headache, flushing and disrupted color vision which result from non-selective effects on a variety of tissues. In spite of these shortcomings, the drug is viewed by patients as preferable to other treatments which involve the introduction of medication directly into the penis via injection, the use of an external device or a surgical procedure.

Dinh et al. Org. Chem. 1975, 40, 2825) describe the preparation of hydroxymethyl-2-furanyl and 5-benzyloxy-2-furanyl pyrazolo[4,3-d]pyrimidin-7ones as by-products and intermediates in the preparation of analogs of formycin B.

Bacon et al., in U.S. Patent No. 5,294,612, describe a series of 6heterocyclyl pyrazolo[3,4-d]pyrimidin-4-one phosphodiesterase inhibitors and their use in treating cardiovascular disease.

Sexually stimulated penile erection results from a complex interplay of physiological processes involving the central nervous system, the peripheral nervous system, and the smooth muscle. Specifically, release of nitric oxide from the non-adrenergic, non-cholinergic nerves and endothelium activates guanylyl cyclase and increases intracellular cGMP levels within the corpus cavernosum. The increase in intracellular cGMP reduces intracellular calcium levels, resulting in trabecular smooth muscle relaxation, which, in turn, results in corporal volume expansion and compression of the sub-tunical venules leading to penile erection.

Agents that increase the concentration of cGMP in penile tissue, either through enhanced release or reduced breakdown of cGMP, are expected to be effective treatments for ED. The intracellular levels of cGMP are regulated by the enzymes involved in its formation and degradation, namely the guanylate cyclases and the cyclic nucleotide phosphodiesterases (PDEs). To date, at least nine families of mammalian PDEs have been described, five of which are capable of hydrolyzing the active, cGMP, to the inactive, GMP, under physiological conditions (PDEs I, II, V, VI, and IX). PDE V is the predominant isoform in human corpus cavernosum. Inhibitors of PDEV, therefore, would be expected to increase the concentration of cGMP in the corpus cavernosum and enhance the duration and frequency of penile erection.

PDEV also has been found in human platelets and vascular smooth muscle, suggesting a role for this enzyme in the regulation of intracellular concentrations of cGMP in cardiovascular tissue. In fact, inhibitors of PDEV have been shown to produce endothelial-dependent vasorelaxation by potentiating the increases in intracellular cGMP induced by nitric oxide.

Moreover, PDEV inhibitors selectively lower the pulmonary arterial pressure in animal models of congestive heart failure and pulmonary hypertension. Hence in addition to their utility in ED, PDEV inhibitors would likely be of therapeutic benefit in conditions like heart failure, pulmonary hypertension, and angina.

Accordingly, it is an object of the invention to identify compounds which inhibit phosphodiesterases, specifically PDEV. It is another object of the invention to identify compounds which are useful for the treatment of sexual dysfunction, particularly erectile dysfunction in male animals. Still another object of the invention is to identify methods for treating sexual dysfunction, especially erectile dysfunction, using the compounds of the present invention.

We now describe a series of 5-heterocyclyl-pyrazolyl[4,3-d]pyrimidin-7ones with the ability to inhibit phosphodiesterase type 5 in an enzyme assay.

Summary of the Invention The present invention is directed to a compound of formula (I) O R 1 HN N

I,/N

SR2 (1) wherein R 1 and R 2 are each independently selected from hydrogen or C 1

-C

6 alkyl; R 3is selected from or R C R 7 6 56r R 5R 6 Sis a five membered heteroaromatic ring which consists of carbon atoms and from one to three heteroatoms selected from N, 0 or S; 41 5, 6 7C R ,R ,R and R7 are each independently selected from hydrogen, C1-0, alkyl, fluorinated C 1

-C

6 alkyl, cyano, (hydroxy)0 1 -Cr, alkyl, 01-06 alkoxy, fluorinated 01-06 alkoxy, C 1

-C

6 alkanoyl, halogen, hydroxy, CI-Gs alkyl-NR 8

NR

8

R',

CONR

8

R

9 or SO 2 NR R9;

R

8 is selected from hydrogen or 01-06 alkyl,

R

9 is selected from hydrogen, 01-06 alkyl, or Cl-Cr, alkyl-NR 13R", or R 8 and R 9 together with the nitrogen to which they are attached form an unsubstituted or substituted pyrrolidinyl, piperidinyl, morpholinyl or 4-N(Rl )-piperazinyl group wherein the substituent is selected from CI-C6 alkyl, 01-06, alkoxy, NR 11

R'

2 or ONR" R' 2

R

10 is selected from hydrogen, 01-06 alkyl, 03-06 cycloalkyl, (hydroxcy)Cl-C6 alkyl, aryl, heteroaryl, aralkyl, aralkenyl or C(0)NH-2; preferably, R 10 is selected from hydrogen, 01-06 alkyl, (hydroxy)0 1

-C

6 alkyl, aryl, heteroaryl, aralkyl, aralkenyl or 0(0)NH2;

R

1 1 and R 1 2 are each independently selected from hydrogen, 01-06 alkyl, (hydroxy)C 1 -0 6 alkyl or (CI-Os, alkoxy)C 1 -Cr, alkyl; and

R

13 and R 1 4 are each independently selected from hydrogen or C1-Cs alkyl, or

R'

13 and R 14 together with the nitrogen to which they are attached form an unsubstituted or substituted pyrrolidinyl, piperidinyl or morpholinyl group wherein the substituent is selected from Ci-C6 alkyl, C1-C6 alkoxy, NR1R 12 or CONR"R1 2 provided that when R 3 is

R

4 O R

R

and R s and R 6 are both hydrogen, then R 4 is selected from hydrogen, Ci-Cs alkyl, fluorinated C1-C6 alkyl, cyano, (hydroxy)C 2 -Cs alkyl, Cl-Cs alkoxy, fluorinated C-C6 alkoxy, Cl-Cs alkanoyl, halogen, hydroxy, Ci-C6 alkyl-NR'R 9

NR"R

9

CONR'R

9 or SO 2

NRSR

9 and pharmaceutically acceptable salts thereof.

In one embodiment of the invention is the compound of formula wherein

R

1 and R 2 are each independently selected from hydrogen and Cl-C4 alkyl;

R

3 is selected from

R

6 or R4 Sis a five membered heteroaromatic ring selected from pyrrolyl, furanyl, thienyl, or imidazolyl;

R

4 and R 6 are each independently selected from hydrogen, C-C4 alkyl, fluorinated Ci-C4 alkyl, cyano, (hydroxy)C1-C 4 alkyl, Ci-C4 alkoxy, fluorinated CI-C4 alkoxy, 01-04 alkanoyl, halogen, hydroxy, 01-04 alkyl-NR 8R 9, NR aR9

CONR

8 R' or SONR"R 9 is selected from hydrogen, 01-04 alkyl or 01-04 alkoxy; R 8 is selected from hydrogen or01-04 alkyl, R9 is selected from hydrogen, 01-04 alkyl, or CI-C 4 alkyl-NR"R 4, or R 8 and R 9 together with the nitrogen to which they are attached form an unsubstituted or substituted pyrrolidinyl, piperidinyl, morpholinyl or 4-N(Rl 0 )-piperazinyl group wherein the substituent is selected from 01-04 alkyl, Cl-C 4 alkoxy, NR"R 12 or0CONR 1 R 12 is selected from hydrogen, C1-04 alkyl, (hydroxy)Cl-C4 alkyl, aryl, heteroaryl, ar(0 1 -C4)alkyl, ar(C 1

-C

4 )alkenyl or C(O)NH- 2 provided that when R 3 /0

-R

and R 5 is hydrogen, then R 4 is selected from hydrogen, 01-04 alkyl, fluorinated

C

1

-C

4 alkyl, cyano, (hydroxy)0 2

-C

4 alkyl, C 1

-C

4 alkoxy, fluorinated 01-04 alkoxy, ClrC4 alkanoyl, halogen, hydroxy, 01-04 alkyl-NR 8

NR

8

CONR

8 R' or

SO

2

NR

8

R

9 and all other variables are as defined above; and pharmaceutically acceptable salts thereof.

In a class of the invention is the compound of formula selected from K or 0 H3 Rs, HN

N,

YH 3 R6

R'

wherein Y is selected from 0, S or N-R 15 where R 5 s is selected from hydrogen and C 1

-C

4 alkyl; and all other variables are as defined above; and pharmaceutically acceptable salts thereof.

Illustrative of the invention is a pharmaceutical composition comprising a pharmaceutically acceptable carrier and any of the compounds described above. An illustration of the invention is a pharmaceutical composition made by mixing any of the compounds described above and a pharmaceutically acceptable carrier. Illustrating the invention is a process for making a pharmaceutical composition comprising mixing any of the compounds described above and a pharmaceutically acceptable carrier.

Exemplifying the invention are methods of treating sexual dysfunction, especially male erectile dyfunction, and/or impotence in a subject in need thereof comprising administering to the subject a therapeutically effective amount of any of the compounds or pharmaceutical compositions described above.

An example of the invention is a method for increasing the concentration of cGMP in penile tissue through the inhibition of phosphodiesterases, specifically PDEV, in a male subject in need thereof comprising administering to the subject an effective amount of any of the compounds or pharmaceutical compositions described above.

Further illustrating the invention is a method of treating a condition selected from the group consisting of heart failure, pulmonary hypertension, and angina in a subject in need thereof comprising administering to the subject a therapeutically effective amount of any of the compounds or pharmaceutical compositions described above.

Further exemplifying the invention is a method of producing endothelialdependent vasorelaxation by potentiating the increases in intracellular cGMP induced by nitric oxide in a subject in need thereof comprising administering to the subject an effective amount of any of the compounds or pharmaceutical compositions described above.

Another example of the invention is the use of any of the compounds described above in the preparation of a medicament for: treating sexual dysfunction, especially male erectile dyfunction, treating impotence, (c) increasing the concentration of cGMP in penile tissue through the inhibition of phosphodiesterases, specifically PDEV, and/or treating a condition selected from the group consisting of heart failure, pulmonary hypertension, and angina, in a subject in need thereof.

Detailed Description of the Invention The present invention provides substituted pyrazolopyrimidinones useful for the treatment of sexual dysfunction, especially ED. Although the compounds of the present invention are useful primarily for the treatment of male sexual dysfunction or erectile dysfunction, they may also be useful for the treatment of female sexual dysfunction related to clitoral disturbances, and of premature labor and dysmenorrhea.

More particularly, the compounds of the present invention are of the formula I O

R

1 HN I

IN

R3/V^N, R RN 2 wherein all variables are as defined above.

The compounds of formula I may exist in tautomeric equilibrium with the corresponding enol form as compounds of formula (VII): OH R 1

)IN

NR2

(VII)

While the compounds are believed to exist predominantly in the keto form as compounds of formula and will be represented as such throughout this specification, it should be understood that the invention contemplates both tautomeric forms, as well as mixtures thereof, and that all such forms and mixtures thereof are included within the scope of the present invention.

For use in medicine, the salts of the compounds of this invention refer to non-toxic "pharmaceutically acceptable salts." Oth-er salts may, however, beuseful in the preparation of compounds according to this invention or of their pharmaceutically acceptable salts. Suitable pharmaceutically acceptable salts of the compounds include acid addition salts which may, for example, be formed by mixing a solution of the compound with a solution of a pharmaceutically acceptable acid such as hydrochloric acid, sulfuric acid, fumaric acid, maleic acid, succinic acid, acetic acid, benzoic acid, citric acid, tartaric acid, carbonic acid or phosphoric acid. Furthermore, where the compounds of the invention carry an acidic moiety, suitable pharmaceutically acceptable salts thereof may include alkali metal salts, sodium or potassium salts; alkaline earth metal salts, calcium or magnesium salts; and salts formed with suitable organic ligands, quaternary ammonium salts. Thus, representative pharmaceutically acceptable salts include the following: acetate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, calcium edetate, camsylate, carbonate, chloride, clavulanate, citrate, dihydrochloride, edetate, edisylate, estolate, esylate, fumarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynaphthoate, iodide, isothionate, lactate, lactobionate, laurate, malate, maleate, mandelate, mesylate, methylbromide, methylnitrate, methylsulfate, mucate, napsylate, nitrate, N-methylglucamine ammonium salt, oleate, pamoate (embonate), palmitate, pantothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, sulfate, subacetate, succinate, tannate, tartrate, teoclate, tosylate, triethiodide and valerate.

The present invention includes within its scope prodrugs of the compounds of this invention. In general, such prodrugs will be functional derivatives of the compounds which are readily convertible in vivo into the required compound. Thus, in the methods of treatment of the present invention, the term "administering" shall encompass the treatment of the various disorders described with the compound specifically disclosed or with a compound which may not be specifically disclosed, but which converts to the specified compound in vivo after administration to the patient. Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in "Design of Prodrugs", ed. H. Bundgaard, Elsevier, 1985.

Where the compounds according to this invention have at least one chiral center, they may accordingly exist as enantiomers. Where the compounds possess two or more chiral centers, they may additionally exist as diastereomers. It is to be understood that all such isomers and mixtures thereof are encompassed within the scope of the present invention.

Furthermore, some of the crystalline forms for the compounds may exist as polymorphs and as such are intended to be included in the present invention.

In addition, some of the compounds may form solvates with water hydrates) or common organic solvents, and such solvates are also intended to be encompassed within the scope of this invention.

The term "subject" as used herein, refers to an animal, preferably a mammal, most preferably a human, who has been the object of treatment, observation or experiment.

The term "therapeutically effective amount" as used herein, means that amount of active compound or pharmaceutical agent that elicits the biological or medicinal response in a tissue system, animal or human that is being sought by a researcher, veterinarian, medical doctor or other clinician, which includes alleviation of the symptoms of the disease or disorder being treated.

The term "alkyl" shall mean straight or branched chain alkanes of one to six carbon atoms, or any number within this range. For example, alkyl radicals include, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, npentyl, 3-(2-methyl)butyl, 2-pentyl, 2-methylbutyl, neopentyl, n-hexyl, 2-hexyl and 2-methylpentyl. Similarly, alkenyl and alkynyl groups include straight and branched chain alkenes and alkynes having 1 to 8 carbon atoms, or any number within this range.

The term "aryl" indicates aromatic groups such as phenyl and naphthyl.

The term "aralkyl" means a Ci-C 6 alkyl group substituted with an aryl group benzyl, phenylethyl). Similarly, the term "aralkenyl" means a Cl-C 6 alkenyl group substituted with an aryl group The term "halogen" shall include iodine, bromine, chlorine and fluorine.

The symbol and the term "five membered heteroaromatic ring", as used herein, represent a stable five membered monocyclic aromatic ring system which consists of carbon atoms and from one to three heteroatoms selected from N, 0 or S. The heteroaromatic ring may be attached at any heteroatom or carbon atom which results in the creation of a stable structure.

Moreover, when the heteroaromatic ring is substituted, by R R 5 or R6 substituents, the substituent(s) may be attached at any carbon atom or heteroatom within the ring which results in the creation of a stable structure.

Examples of five membered heteroaromatic rings include, but are not limited to, furanyl, imidazolyl, isoxazolyl, oxazolyl, pyrazolyl, pyrrolyl, thienyl, thiazolyl, thiadiazolyl and triazolyl.

The symbol represents a nine membered benzo-fused heteroaromatic ring system which consists of carbon atoms and from one to three heteroatoms selected from N, 0 or S. The benzo-fused heteroaromatic ring may be attached at any heteroatom or carbon atom which results in the creation of a stable structure. Moreover, when the benzo-fused heteroaromatic ring is substituted, by R 4

R

5

R

6 or R 7 substituents, the substituent(s) may be attached at any carbon atom or heteroatom within the ring which results in the creation of a stable structure. Examples of nine membered benzo-fused heteroaromatic rings include, but are not limited to, benzimidazolyl, benzofuranyl, benzothienyl, benzisoxazolyl, benzoxazolyl, indazolyl, indolyl, benzothiazolyl, benzothiadiazolyl and benzotriazolyl.

The term "heteroaryl" as used herein represents a stable five or six membered monocyclic aromatic ring system or a nine or ten membered benzofused heteroaromatic ring system which consists of carbon atoms and from one to three heteroatoms selected from N, 0 or S. The heteroaryl group may be attached at any heteroatom or carbon atom which results in the creation of a stable structure. Examples of heteroaryl groups include, but are not limited to pyridyl, pyrimidinyl, thienyl, furanyl, imidazolyl, isoxazolyl, oxazolyl, pyrazolyl, pyrrolyl, thiazolyl, thiadiazolyl, triazolyl, benzimidazolyl, benzofuranyl, benzothienyl, benzisoxazolyl, benzoxazolyl, indazolyl, indolyl, benzothiazolyl, benzothiadiazolyl, benzotriazolyl, quinolinyl or isoquinolinyl. Preferred heteroaryl groups include pyridyl, pyrimidinyl, thiazolyl, imidazolyl, benzimidazolyl, quinolinyl and isoquinolinyl.

Under standard nomenclature used throughout this disclosure, the terminal portion of the designated side chain is described first, followed by the adjacent functionality toward the point of attachment. Thus, for example, a "phenylCi-C6 alkylamidoC 1

-C

6 alkyl" substituent refers to a group of the formula:

C-C

6 alkyC alk As used herein, the term "composition" is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combinations of the specified ingredients in the specified amounts.

It is intended that the definition of any substituent or variable R 1 0 in Claim 3) at a particular location in a molecule be independent of its definitions elsewhere in that molecule. It is understood that substituents and substitution patterns on the compounds of this invention can be selected by one of ordinary skill in the art to provide compounds that are chemically stable and that can be readily synthesized by techniques known in the art as well as those methods set forth herein.

The utility of the compounds to treat ED can be determined according to the procedure described in Example 4 herein. Additionally, methods for determining the cGMP PDE and cAMP PDE inhibitory activities of the compounds, which are indicative of their utility for treating ED, are described in WO 93/06104, WO 93/07149, WO 93/12095, WO 94/00453, WO 94/05661, and WO 96/16657.

The present invention therefore provides a method of treating erectile dysfunction in a subject in need thereof which comprises administering any of the compounds as defined herein in a quantity effective to treat ED. The compound may be administered to a patient by any conventional route of administration, including, but not limited to, intravenous, oral, subcutaneous, intramuscular, intradermal and parenteral. The quantity of the compound which is effective for treating ED is between 0.1 mg per kg and 20 mg per kg of subject body weight.

The present invention also provides pharmaceutical compositions comprising one or more compounds of this invention in association with a pharmaceutically acceptable carrier. Preferably these compositions are in unit dosage forms such as tablets, pills, capsules, powders, granules, sterile parenteral solutions or suspensions, metered aerosol or liquid sprays, drops, ampoules, autoinjector devices or suppositories; for oral parenteral, intranasal, sublingual or rectal administration, or for administration by inhalation or insufflation. Alternatively, the composition may be presented in a form suitable for once-weekly or once-monthly administration; for example, an insoluble salt of the active compound, such as the decanoate salt, may be adapted to provide a depot preparation for intramuscular injection. For preparing solid compositions such as tablets, the principal active ingredient is mixed with a pharmaceutical carrier, e.g. conventional tableting ingredients such as corn starch, lactose, sucrose, sorbitol, talc, stearic acid, magnesium stearate, dicalcium phosphate or gums, and other pharmaceutical diluents, e.g. water, to form a solid preformulation composition containing a homogeneous mixture of a compound of the present invention, or a pharmaceutically acceptable salt thereof. When referring to these preformulation compositions as homogeneous, it is meant that the active ingredient is dispersed evenly throughout the composition so that the composition may be readily subdivided into equally effective dosage forms such as tablets, pills and capsules. This solid preformulation composition is then subdivided into unit dosage forms of the type described above containing from to about 1000 mg of the active ingredient of the present invention. The tablets or pills of the novel composition can be coated or otherwise compounded to provide a dosage form affording the advantage of prolonged action. For example, the tablet or pill can comprise an inner dosage and an outer dosage component, the latter being in the form of an envelope over the former. The two components can be separated by an enteric layer which serves to resist disintegration in the stomach and permits the inner component to pass intact into the duodenum or to be delayed in release. A variety of material can be used for such enteric layers or coatings, such materials including a number of polymeric acids with such materials as shellac, cetyl alcohol and cellulose acetate.

The liquid forms in which the novel compositions of the present invention may be incorporated for administration orally or by injection include, aqueous solutions, suitably flavoured syrups, aqueous or oil suspensions, and flavoured emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil or peanut oil, as well as elixirs and similar pharmaceutical vehicles. Suitable dispersing or suspending agents for aqueous suspensions, include synthetic and natural gums such as tragacanth, acacia, alginate, dextran, sodium carboxymethylcellulose, methylcellulose, polyvinyl-pyrrolidone or gelatin.

Where the processes for the preparation of the compounds according to the invention give rise to mixture of stereoisomers, these isomers may be separated by conventional techniques such as preparative chromatography. The compounds may be prepared in racemic form, or individual enantiomers may be prepared either by enantiospecific synthesis or by resolution. The compounds may, for example, be resolved into their component enantiomers by standard techniques, such as the formation of diastereomeric pairs by salt formation with an optically active acid, such as (-)-di-p-toluoyl-d-tartaric acid and/or toluoyl-l-tartaric acid followed by fractional crystallization and regeneration of the free base. The compounds may also be resolved by formation of diastereomeric esters or amides, followed by chromatographic separation and removal of the chiral auxiliary. Alternatively, the compounds may be resolved using a chiral HPLC column.

During any of the processes for preparation of the compounds of the present invention, it may be necessary and/or desirable to protect sensitive or reactive groups on any of the molecules concerned. This may be achieved by means of conventional protecting groups, such as those described in Protective Groups in Organic Chemistry, ed. J.F.W. McOmie, Plenum Press, 1973; and T.W. Greene P.G.M. Wuts, Protective Groups in Organic Synthesis, John Wiley Sons, 1991. The protecting groups may be removed at a convenient subsequent stage using methods known from the art.

The method of treating ED described in the present invention may also be carried out using a pharmaceutical composition comprising any of the compounds as defined herein.and a pharmaceutically acceptable carrier. The pharmaceutical composition may contain between about 5 mg and 1000 mg, preferably about 10 to 500 mg, of the compound, and may be constituted into any form suitable for the mode of administration selected. Carriers include necessary and inert pharmaceutical excipients, including, but not limited to, binders, suspending agents, lubricants, flavorants, sweeteners, preservatives, dyes, and coatings. Compositions suitable for oral administration include solid forms, such as pills, tablets, caplets, capsules (each including immediate release, timed release and sustained release formulations), granules, and powders, and liquid forms, such as solutions, syrups, elixers, emulsions, and suspensions. Forms useful for parenteral administration include sterile solutions, emulsions and suspensions.

Advantageously, compounds of the present invention may be administered in a single daily dose, or the total daily dosage may be administered in divided doses of two, three or four times daily. Furthermore, compounds for the present invention can be administered in intranasal form via topical use of suitable intranasal vehicles, or via transdermal skin patches well known to those of ordinary skill in that art. To be administered in the form of a transdermal delivery system, the dosage administration will, of course, be continuous rather than intermittent throughout the dosage regimen.

For instance, for oral administration in the form of a tablet or capsule, the active drug component can be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water and the like. Moreover, when desired or necessary, suitable binders, lubricants, disintegrating agents and coloring agents can also be incorporated into the mixture. Suitable binders include, without limitation, starch, gelatin, natural sugars such as glucose or betalactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like. Disintegrators include, without limitation, starch, methyl cellulose, agar, bentonite, xanthan gum and the like.

The liquid forms may include suitably flavored suspending or dispersing agents such as the synthetic and natural gums, for example, tragacanth, acacia, methyl-cellulose and the like. For parenteral administration, sterile suspensions and solutions are desired. Isotonic preparations which generally contain suitable preservatives are employed when intravenous administration is desired.

The compound of the present invention can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles, and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, such as cholesterol, stearylamine or phophatidylcholines.

Compounds of the present invention may also be delivered by the use of monoclonal antibodies as individual carriers to which the compound molecules are coupled. The compounds of the present invention may also be coupled with soluble polymers as targetable drug carriers. Such polymers can include polyvinylpyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamidephenol, polyhydroxyethylaspartamidephenol, or polyethyl-eneoxidepolylysine substituted with palmitoyl residue. Furthermore, the compounds of the present invention may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates and cross-linked or amphipathic block copolymers of hydrogels.

Compounds of this invention may be administered in any of the foregoing compositions and according to dosage regimens established in the art whenever treatment of ED is required.

The daily dosage of the products may be varied over a wide range from to 1,000 mg per adult human per day. For oral administration, the compositions are preferably provided in the form of tablets containing, 5.0, 10.0, 15.0, 25.0, 50.0, 100, 250 and 500 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated. An effective amount of the drug is ordinarily supplied at a dosage level of from about 0.1 mg/kg to about mg/kg of body weight per day. Preferably, the range is from about 0.2 mg/kg to about 10 mg/kg of body weight per day, and especially from about 0.5 mg/kg to about 10 mg/kg of body weight per day. The compounds may be administered on a regimen of 1 to 4 times per day.

Optimal dosages to be administered may be readily determined by those skilled in the art, and will vary with the particular compound used, the mode of administration, the strength of the preparation, and the advancement of the disease condition. In addition, factors associated with the particular patient being treated, including patient age, weight, diet and time of administration, will result in the need to adjust dosages.

Abbreviations used in the specification, particularly the Schemes and Examples, are as follows: DMAP dimethylaminopyridine DMF dimethylformamide DMSO dimethylsulfoxide Et 3 N triethylamine EtOH ethanol MeOH methanol r.t. room temperature TEA triethylamine The compounds of the invention may be prepared by the reaction of a compound of the general formula (II): O

R

1 HN N

N

R 2 0 11 where R 1 and R 2 are as previously defined, and is a suitably substituted five membered heteroaromatic ring or a suitably substituted nine membered benzo-fused heteroaromatic ring as defined for R 3 above, with a suitably substituted amine as defined for R 8 and R 9 above. The reaction is generally carried out a room temperature, preferably in the presence of a solvent, for example an alkanol containing one to three carbon atoms, using an excess of the amine to scavenge the acid by-product, HCI.

Compounds of the general formula (II) may be prepared from compounds of the general formula (III): 0 Ri

R'

2 Ill by reacting with chlorosulfonic acid at temperatures ranging from -20 0 C to 50 0

C

depending on the reactivity of the heterocyclic moiety, Compounds of general formula (111) may be prepared from compounds of the general formula (IV): 0

R'

H

2 N N

IV

HN

by the application of known cyclization methods for pyrimidinone ring formation. Thus, for example, the cyclization may be effected by the treatment of (IV) with a base such as sodium hydroxide or potassium carbonate in an ethanol-water medium at reflux temperature for 2-65 hours. Alternatively, compounds of the general formula (111) may also be obtained by treatment of (IV) with polyphosphoric acid at or near 140°C for 6-18 hours.

Compounds of the general formula (IV) may be prepared from compounds of the general formula O R 1

H

2

N

H

2 N 2

V

by reaction with a compound of general formula (VI): 0

VI

wherein is as previously defined. The reaction is generally carried out using an excess of (VI) in the presence of an excess of an aliphatic tertiary amine such as triethylamine to act as scavenger for the acid by-product (HC1), optionally in the presence of a catalyst such as 4-dimethylaminopyridine, in an inert solvent such as dichloromethane at 0°C to room temperature for 2-24 hours.

The amines used in the reaction with (11) to form the compounds of the invention, the aminopy razoles of formula and the acyl chlorides of formula when not commercially available, can be readily obtained by one of ordinary skill in the art by conventional synthetic procedures, in accordance with literature precedent, from readily accessible starting materials using standard reagents and reaction conditions.

As a further illustration of the synthesis of the compounds of the invention, the preparation of 5-13-ethoxy-5-(4methylpiperazinylsulfonyl)th iophene-2-y)-1 -methyl-3-n-p ropyl-6 ,7-d ihydro-1 Hpyrazolo-[4 ,3-dlpyrimidin-7-o ne and 5-[3-hydroxy-5-(4methylpiperazinylsu lfonyl)thiophene-2-yl)-1 -methyl-3-n-propyl-6 ,7-dihydro-1 Hpyrazolo-[4,3-d]pyrimidin-7-one is detailed in Scheme 1. Thus, the starting material, pyrazole I was reacted with the thiophenecarbonyl chloride 2 to give the intermediate 3. The cyclization of 3 was effected with sodium hydroxide in ethanol to give 4. Chlorosulfonylation followed by reaction with Nmethylpiperazine gave compound 5 and compound 6.

H

3

C

NH

2

N

SNH

2 1- 0C 2

H

5 I\ 0 C1

H

3 C 00 2

H

N H NI N\ 0 6H 3

NH

2

TEAIDMAP

CH

2

CI

2 NaOH EtCH HN N S s N 1) CIS0 3

H

CH

3 2) H 3 C-N %-/NH 4 o CH 3

HN

HN

~.N

0 C' 6 Scheme I The following Examples are set forth to aid in the understanding of the invention, and are not intended and should not be construed to limit in any way the invention set forth in the claims which follow thereafter.

Unless otherwise indicated, H NMRs were run on a 300 MHz Bruker AC 300 instrument.

Example 1 5-[3-Ethoxy-5-(4-methylpiperazinylsulfonyl)thiophene-2-y)-l -methyl-3-npropyl-6,7-dihydro-I H-pyrazolo-[4,3-d]pyrimidin-7-one and 54[3hydroxy-5-(4-methylpiperazinylsulfonyl)thiophene-2-y)-l -methyl-3-npropyl-6,7-dihydro-1 H-pyrazolo-[4,3-d]pyrimidin-7-one (6) a. 4-(3-Eth oxythi op hene-2-ca rboxa mid 1 -methvl-3-n-p carboxamide (3) 3-Ethoxythiophene-2-carbonyi chloride 2, prepared from the reaction of 3-ethoxythiophene-2-carboxylic acid (2.79 g, 16.2 mmol) with oxalyl chloride, was added to a solution of 4-amino-1-methyl-3-n-propylpyrazole-5carboxamide 1 (2.08 g, 11.4 mmol), DMAP (28 mg, 0.23 mmol) and Et 3 N (1.64 g, 16.2 mmot) in anhydrous CH 2

CI

2 (30 mL) at 0 and stirred at r.t. for 24 h.

The- mixture- Was-Washed-with-T N -HCI-and d fied- with- Mg SOZ -Chromatog raphy ic5b L-el a n e-:thyF Nacetfiae Y1 gave *a wif K6sb-Olid;Tpi1010 0

H-

NMR (DMSO-d6) 8 0.87 J =7 Hz, 3 1.39 J =7 Hz, 3 1.56 (sxt, J 7 Hz, 2 2.43 J 7 7Hz, 2 3.88 3 4.32 J 7 Hz, 2 7.18 (d, J 6 Hz, 1 7.66 br. I 7.75 br. 1 7.81 J =6 Hz, 1 8.87 1 MS (mlz): 337 b) 5-(3-Ethoxythiorphene-2-fl)-lI-methyl-3-n-propyl-6 .7-dihvdro-1 H-pyrazolor4,3-d~nyrimidin-7-one (4) A mixture of 3 (1.15 g, 3.42 mmol), NaOH (284 mg, 7.1 mmol) in ethanol (11 mL) and water (34 mL) was heated at 80 °C for 65 h. After cooling to r.t. a white precipitate was formed. Filtration and drying under vacuum gave 0.625 g of the product. The filtrate was extracted with CH 2

CI

2 and the organic phase was washed with 1N HCI and brine, dried with MgSO 4 Evaporation of the solvent gave additional product; mp: 151-152 OC; 1 H NMR (CDCI3) 8 1.00 J 7 Hz, 3 1.54 J 7 Hz, 3 1.82 (sxt, J= 7 Hz, 2 2.86 J 7 Hz, 2 4.23 3 4.34 J 7 Hz, 2 6.87 (d, J= 6 Hz, 1 7.37 J= 6 Hz, 1 MS (mlz): 319 c. 5- 3-Ethoxy-5-(4-methylpiperazinylsulfonyl)-thiophene-2-yll-1 -methyl-3n-propyl-6,7-dihvdro-1H-pyrazolo-14,3-d]pyrimidin-7-one and 5-r3-hydrox- 5-(4-methyvlpiperazinvlsulfonvl)-thiophene-2-vil-1-methyl-3-n-propyl-6,7-dihydro- 1H-pyrazolo-[4,3-d]pyrimidin-7-one (6) Compound 4 (165 mg, 0.517 mmol) was added to chlorosulfonic acid (0.35 mL, 5.2 mmol) at 0 The mixture was stirred under nitrogen at 50 °C for 24 h. Ice was added and the mixture was extracted with a mixture of

CH

2 Cl 2 /MeOH dried with Na 2

SO

4 After evaporation of the solvent, a white solid was obtained which, by NMR analysis contained predominantly (5-chlorosulfonyl-3-ethoxythiophene-2-yl)-1-methyl-3-n-propyl-6,7-dihydro-1Hpyrazolo-[4,3-d]pyrimidin-7-one and a small amount of 5-(5-chlorosulfonyl-3hydroxythiophene-2-yl)-1-methyl-3-n-propyl-6,7-dihydro-1H-pyrazolo-[4,3d]pyrimidin-7-one. This white solid (90 mg, 0.456 mmol) was added to a solution of methylpiperazine (137 mg, 1.37 mmol) in methanol (abs. 15 mL) and stirred at rt for 4 days. The solvent was evaporated, and the residue was dissolved in CH 2 CI2/MeOH and washed with saturated Na 2

CO

3 The aqueous layer was extracted with CH 2

CI

2 /MeOH The organic layers were combined and dried with MgSO 4 The residue obtained after evaporation of the solvent contained two compounds. Separation and purification was carried out by column chromatography (silica gel). Compound 5 was obtained as a white solid by elution with ethyl acetate; mp: 219-220 IH NMR (DMSO-d6) 8 0.94 J= 7 Hz, 3 1.42 J= 7 Hz, 3 1.74 (sxt, J= 7 Hz, 2 2.18 3 2.40 (in, 4 2.76 J 7 Hz, 2 3.35 (in, 4 4.14 3 4.44 J =7 Hz, 2 7.72 1 10.79 (br s, 1 MS (mlz): 481 The hydrochloride salt was prepared by adding a solution of HCI in dioxane (4.0 M, 0.2 ml-) to a suspension of the free base (6.0 mg) in MeOH (1 ml-) and stirring for 1 h. Following removal of the solvents under vacuum a white solid was obtained; mp: 225 IC (dec.).

Compound 6 was obtained as a white solid by elution with methanol in ethyl acetate. The hydrochloride salt was prepared by adding a solution of HCl in dioxane (4.0 M, 0.2 mL) to a suspension of the free base mg) in MeOH (1 mL) and stirring for 1 h. Following removal of the solvents under vacuum a white solid was obtained; mp: 257 OC 1H NMVR (DMSOd 6 5 0.95 J 7 Hz, 3 1.75 J 7 Hz, 2 2.49 3H), 2.70 (mn, 6 H), 3.15 (in, 4 4.15 3 7.61 1 8.45 1 10.85 1 MS (mlz) 453 (MH* 4 451 (MHD.

Example 2 5-(7-Ethoxybenzofuran-2-yI)-1 -methyl-3-n-propyl-6,7-dihydro-1 H-pyrazolo- [4,3-djpyrimidin-7-one (7) A. 4-(7-Ethoxvbenzofuran-2-carboxamido)-1 -methVl-3-n-p carboxamide Fro-m 7-ethox ybeniofur-a n-2-carbonyl chloride (4.124 g,20 m mol), I (1.2 g, 10 minol),- DMAP (32.5 mg, 0.27 inmol) and Et 3 N (2.02 g. 20 rnmol) in anhydrous CH 2

CI

2 (65 mL) using the same procedure as for 3 the title compound was obtained as a white solid; mp: 219-221 00; 1 H NMR (DMSO-d6) 5 0.86 J 7 Hz, 3 1.43 J =7 Hz, 3 1.56 (sxt,- J 7 Hz, 2 2.42 J 7 Hz, 2 3.91 3 4.28 J 7 Hz, 2 H), 7. 10(d, J =6 Hz, 1 71.26 J =6 Hz, 1 7.35 J =6 Hz, I 7.50 (s, br. I 7.71 1 7.76 br. I MS (mlz): 371 b. 5-(7-Ethoxvbenzofuran-2-yl)-l -methyl-3-n-propvl-6,7-d ihydro-l H- Pvrazolo4[4,3-dlpvrimidin-7-one (7) From 4-(7-ethoxybenzofura n-2-carboxa mido)- I -methyl-3-npro pylpyrazole-5-carboxamnide (281.5 mg, 0.76 mmol) and NaOH (63 mg, 1.573 mmol) in MeOH/H 2 0 (10 mL/6.5 mL) using the procedure for 4 the title compound 7 was obtained as a white solid; mp: 227-229 I H NMVR (DMSOd6) 860.98 J =7 Hz, 3 1.46 J 7 Hz, 3 1.78 (sxt, J =7 Hz, 2 H), 2.84 J 7 Hz, 2 4.17 3 4.29 J 7 Hz, 2 7.06 J 6 Hz, 1 7.24 J =6 Hz, 1 7.31 J 6 Hz, 1 7.92 I MS (mlz): 353 'Example 3 The sulfonyl chloride precursors to compounds 8, 9, 11, and 32 could be prepared essentially as described in Example I c with the following provisos: f- 1) 5-(4-chlorosulfonyl1-7-e.thoxybenz.ofuran-2-y)-1 -methyl-3-n-propyl-6,7dihydro-1 H-pyrazolo-[4 ,3-d]pyrimidin-7-one was obtained by reaction of 5-(7-ethoxybenzofuran-2-y)-1 -methyl-3-n-propyl-6,7-dihyd ro-1 Hpyrazolo-[4,3-d]pyrimidin-7-one with 12 equivalents of chlorosulfonic acid at -7 to 0 0 IC for 3 h; 2) 5-4,6-bis-(chlorosulfonyl)-7-ethoxybenzofuran-2-yJ-1 -methyl-3-n-propylq,7-dihydro-1 H-pyrazolo-[4 ,3-d]pyrimidin-7-one was obtained by reaction of 5-(7-ethoxybenzofuran-2-y)-1 -methyl-3-n-pr .opyl-6 ,7-d ihyd ro-1 Hpyrazolo-[4,3-d]pyrimidin-7-one with 12 equivalents of chlorosulfonic acid at 20 0 'C for 18 h; and 3) 5-(4-chlorosulfonyf-1 -methylpyrrol-2-yl)-l1-methyl-3-n-propyl-6 ,7-d ihydro- 1 H-pyrazolo-[4,3-d]pyrimidin-7-one was obtained by reaction of 5-(1methylpyrrol-2-y)-1 -methyl-3-n-propyl-6 ,7-dihydro-1 H-pyrazolo-[4 ,3d]pyrimidin-7-one with 100 equivalents of chlorosulfonic acid at 20 OC for 4 days.

Example 4 As a specific embodiment of an oral composition, 100 mg of the compound 5 of Example 1 is formulated with sufficient finely divided lactose to provide a total amount of 580 to 590 mg to fill a size 0 hard gel capsule.

Following the procedures described herein, the following compounds in Table 1 were prepared.

TABLE 1 HN N K I

CH

3 Compound R 3 Molecular [on 4 S 319 (M4-H) 0C 2

H-

00 N C11 481 6 09s N' 453 (M+H) CD

OH

7 0C 2 1- 0 353 (M4-H) 8 0C 2

H

*--O515 (M+H)

H

3

C-N

Compound ComoundMolecular Ion H3CS N 9 N 0 2 H 677 (M+H) 9 0,

IN'!

H

3 C

N

H

3 Cl 272 (M+H) 11

H

3

C

N

434

(M+H)

H

3 c 12 0 0 tS I545 (M+H)

C

2

H

N

compound Molecular Ion 14 0 s529

(M+H)

It s

OH

N

517 (M+H) O~ S CN

OH

ND

16 0 s543

(M+H)

0.-Z 17 0515

(M+H)

(N

OH

583 (M+H) Compound R3Molecular Ioni 19 0

CN'

D

OH

o 0s 509 (M+H)

N'

N C 2

HS

21 0 s481

(M+H)

N-

22 0 s 426 (M+H) -N 0C 2

HS

24 .0495 Compound R 3 0 D

OH

OH

3 0

-N'

j 0C 2

H

N

-N

OH

N

Molecular Ion 467 (M+H) 483 (M4-H) 455 (M+H) 275 (M+H) 509 (M+H) 549 (Mi-H) 521 (Mi-H) 0 0 Compound R' Molecular Ion 32 H 3 C 496 (M+H)

"S,

o

N

N

N=K

Example As mentioned previously, it is understood by those skilled in the art that compounds which enhance the release or inhibit the breakdown of cGMP from corpus cavernosum tissue will promote penile erection. Therefore, a method is described to measure the ability of the compounds to increase nitric oxide (NO)-stimulated cGMP levels in penile tissue.

Biological Methods Measurement of cGMP Levels in Corpus Cavernosum Tissue Rings Tissue Preparation Male New Zealand White rabbits (2.5 kg) are killed with an overdose of pentobarbitorteand their penises-excised rapidly Epidermal and connectivetissu-is ert Ioived:U ~id peises cUL IlgitLUUdiIally into two equal 4 U Lstrips and th 2 1-_ laterally to give-about20 segments:-The urethra is carefully dissected out and discarded. These segments of corpora cavemosa from all animals are pooled and incubated in Dulbecco's minimum essential medium (MEM) at 37°C for 1 h with regular changes of medium to allow the tissues to recover from preparative handling.

Effect of test compound on cGMP formation Tissues are prepared as above; two penile discs for each drug dose are incubated in 200 pL of MEM with various concentrations of test compound and sodium nitroprusside (SNP) added (10 [pmol/L final concentration). SNP breaks down spontaneously in aqueous solution to generate NO. After preincubation of the tissues with the compounds for 10 min at 370C, cGMP formation is stimulated with various concentrations of SNP. The tubes are then incubated at 37 0 C for a further 30 min and the reaction stopped by adding 200 liL of 1 mol/L perchloric acid. The tissues are ultrasonicated (3 x20 s bursts; MSE Soniprep) and the residual tissue pelleted by centrifugation. After centrifugation, aliquots of supernatant are taken and neutralized with 0.5 mol/L

K

3 P0 4 After appropriate dilution in assay buffer, cGMP concentrations are measured by radioimmunoassay (Amersham dual-range 1251 kit) following the manufacturer's instructions. The lower limits of detection are 1 fmol for cGMP assays.

Cyclic Nucleotide Phosphodiesterase (PDE) Assay PDE Isolation PDEs were isolated from rabbit tissues according to the protocol described by Boolell et al. in International Journal of Impotence Research 1996, 8, 47-52 with minor modifications. Basically, the tissues were homogenized in an ice-cold buffer containing 20mM HEPES (pH 0.25M sucrose, 1mM EDTA, and 1mM phenylmethyl sulfonylfluoride (PMSF). The homogenates were centrifuged at-1 00,000g for 60 minutes at 4 C. Thesupernatat was filtered through a 0.2M filter and loaded on a Pharmacia- Mono Q anion exchange column (Iml bed volume) that was equilibrated with HEPES, ImM EDTA and 0.5mM PMSF. After washing out unbound proteins, the enzymes were eluted with a linear gradient of 100-500 mM NaCl in the same buffer (35 to 60 ml total, depending on the tissue). The column was run at th flow rate of Iml/min and 1ml fractions were collected. The fractions comprising various PDE activities were pooled separately and used in later studies.

Measurement of Inhibition of PDE V The PDE assay was carried out essentially as described by Thompson and Appleman in Biochemistry 1971, 10, 311-316 with minor modifications. Briefly, the enzyme was assayed in 5mM MgC1 2 15mM Tris HCI (pH 0.5 mg/ml bovine serum albumin, 1 pM cGMP or cAMP, 0.1 pgCi 3 H]-cGMP or H]-cAMP, and 2-10 jI of column elution. The total volume of the assay was 100 pi. The reaction mixture was incubated at 300°C for 30 minutes. The reaction was stopped by boiling for 1 minute and then cooled on ice. The resulting 3 mononucleotides were further converted to uncharged 3 H]-nucleotides by adding 25 pl 1 mg/mi snake venom (Ophiophagus hannah) and incubating at 30 0 C for 10 minute. The reaction was stopped by the addition of 1 ml Bio-Rad AG1-X2 resin slurry All the charged nucleotides were bound by the resin and only the 3 H]-nucleotides remained in the supernatant after centrifuging.

An aliquot of 350 pi was taken and counted by liquid scintillation. PDE activity was expressed as pmol cyclic nucleotide hydrolyzed/min/ml of enzyme preparation. Inhibitor studies were carried out in assay buffer with a final concentration of 10% DMSO. Under these conditions, the hydrolysis of substrate did not exceed 15%. The formation of product increased with time and enzyme concentration in a linear fashion.

PDEV inhibitory activities for representative compounds of the present invention are described in Table 2. Data is presented either as the IC50 (JM) or as a _percent inhibition at a given concentration of test compound.

TABLE 2 Compound Activity Activity ICso Inhibition% at (cone. [M) 4 0.47 8 6 7 0.91 8 5.4 9 6(20) 11 31(10) 12 1.2 13 46(20) 14 18(20) 13(20) 16 19(10) 17 28(10) 18 44(10) 19 27(10) 0.95 21 30(10) 22 1.4 23 30(10) 24 2.3 26(10) 26 4.9 27 32(10) 28 1.6- 29 8.3 33(10) 31 31(10) 32 31(10) While the foregoing specification teaches the principles of the present inventionTw.,ith-examples.-provided-for-the-pu rpon o-ilsrto~twl-e udertoo tat the nraC~tice ofthie in entio~n Pnrompasses all of the usualvariationis, adaptations and/or modifications as come within the scope of the f6l6w-i liff aind their equivalents.

Claims (13)

1. A compound of formula (I) wherein R 1 and R 2 are each independently selected from hydrogen or C 1 -C 6 alkyl; R 3 is selected from is a five membered heteroaromatic ring which consists of carbon atoms and from one to three heteroatoms selected from N, 0 or S; R 4 R 5 R 6 and R 7 are each independently selected from hydrogen, Cl-C 6 alkyl, .fluorinated, C 1 -C 6 alkyl,.cyano,.(hydroxy)C-C6, alkyl, -C 6 a!koxy, fluorinatecd Ci-C 6 alkoxy, Cl-CE alkanoyl, halogen, hydroxy, C 1 -Cs alkyl-NR 8 R 9 NR 8 R 9 CON R 8 9 or SO 2 NR 8 R 9 R 8 is selected from hydrogen or Cl-C 6 alkyl, R 9 is selected from hydrogen, Ci-C 6 alkyl, or Ci-C 6 alkyl-NR"R 1 4 or R 8 and R 9 together with the nitrogen to which they are attached form an unsubstituted or substituted pyrrolidinyl, piperidinyl, morpholinyl or

4-N(Rl 0 )-piperazinyl group wherein the substituent is selected from C 1 -C 6 alkyl, Ci-C 6 alkoxy, NR11R 12 or CONR"R12; R 10 is selected from hydrogen, C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl, (hydroxy)C 1 -C 6 alkyl, aryl, heteroaryl, aralkyl, aralkenyl or C(O)NH 2 and R 1 and R 12 are each independently selected from hydrogen, C 1 -C 6 alkyl, (hydroxy)C 1 -C 6 alkyl or (Ci-C 6 alkoxy)Ci-C 6 alkyl; and R 13 and R 14 are each independently selected from hydrogen or Ci-C 6 alkyl, or R 13 and R 14 together with the nitrogen to which they are attached form an unsubstituted or substituted pyrrolidinyl, piperidinyl or morpholinyl group; provided that when R 3 is R R and R s and R 6 are both hydrogen, then R 4 is selected from hydrogen, Cl-C 6 alkyl, fluorinated Ci-C 6 alkyl, cyano, (hydroxy)C 2 -Cs alkyl, Cl-C 6 alkoxy, fluorinated Ci-Cs alkoxy, C 1 -C 6 alkanoyl, halogen, hydroxy, C,-C 6 alkyl-NRR 9 NRR' 9 CONR'R 9 or SO 2 NR 8 R 9 and pharmaceutically acceptable salts thereof. 2. The compound of Claim 1, wherein R- and R 2 are each independently selected from hydrogen and CI-C 4 alkyi; R 3 is selected from R 6 or R4 P 4 5 R e is a five membered heteroaromatic ring selected from pyrrolyl, furan yl, thienyl, or imidazolyl; R 4 and R6 are each independently selected from hydrogen, 01-04 alkyl, fluorinated 01-04 alkyl, cyano, (hydroxy)C 1 -C 4 alkyl, 01-04 alkoxy, fluorinated 01-04 alkoxy, 01-04 alkanoyl, halogen, hydroxy, cl-c 4 alkyl-NR 8 NR 8 CONR 8 R 9 or S 2 NR 8 R 9 R 5 is selected from hydrogen, 0 1 -C 4 alkyl or 01-04 alkoxy; 8ai eetdfo ydoe rC-4akl R9 is selected from hydrogen, or0-4 alkyl, o 01-04 alkyl-NR 3 R 14 or Ra and R9 together with the nitrogen to which they are attached form an unsubstituted or substituted pyrrolidinyl, piperidinyl, morpholinyl or 4-N(Rl 0 )-piperazinyl group wherein the substituent is selected from C 1 -C 4 alkyl, 01-04 alkoxy, NR 1R Uor CONR R 12 and R1 0 is selected from hydrogen, 01-04 alkyl, (hydrOXY)Cl-C4 alkyl, aryl, eera 01ar. -C Kya.cy kr(C 1 4a 1 kyio JM '2.7 provided- that when R 3 is_ C; R and RW is hydrogen, then R 4 is selected from hydrogen, 01-04 alkyl, fluorinated 01-04 alkyl, cyano, (hydroxy)C 2 -C 4 alkyl, 01-04 alkoxy, fluorinated 01-04 alkoxy, 01-04 alkanoyl, halogen, hydroxy, 01-04 alkyl-NR NR 8 CONR 8 R' or SO 2 NR8R9; and pharmaceutically acceptable salts thereof. 3. The compound of Claim 2 selected from 0 HN NN 4 H 3 Ror wherein Y is selected from 0, S or N-R15 where R' 5 is selected from hydrogen and Ci-C 4 alkyl; and pharmaceutically acceptable salts thereof. 4. The compound of Claim 1, wherein Sht al kylraC()NH and pharmaceutically acceptable salts thereof. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and a compound of Claim 1.

6. A pharmaceutical composition made by mixing a compound of Claim 1 and a pharmaceutically acceptable carrier.

7. A process for making a pharmaceutical composition comprising mixing a compound of Claim 1 and a pharmaceutically acceptable carrier.

8. A method of treating sexual dysfunction in a subject in need thereof comprising administering to the subject a therapeutically effective amount of the compound of Claim 1.

9. The method of treating sexual dysfunction of Claim 8, wherein the sexual dysfunction is male erectile dysfunction. A method of treating sexual dysfunction in a subject in need thereof comprising administering to the subject a therapeutically effective amount of the composition of Claim

11. The method of treating sexual dysfunction of Claim 10, wherein the sexual dysfunction is male erectile dysfunction.

12. A method of treating impotence in a male subject in need thereof comprisingadministering to the subject a therapeutically effective amount of the compound of Claim 1.

13. A method of treating impotence in a male subject in need thereof comprising administering to the subject a therapeutically effective amount of the composition of Claim

14. A method for increasing the concentration of cGMP in penile tissue in a male subject in need thereof comprising administering to the subject an effective amount of the compound of Claim 1.

15. A method for increasing the concentration of cGMP in penile tissue in a male subject in need thereof comprising administering to the subject an effective amount of the composition of Claim

16. A method of treating a condition selected from the group consisting of heart failure, pulmonary hypertension, and angina in a subject in need thereof comprising administering to the subject a therapeutically effective amount of the compound of Claim 1.

17. A method of treating a condition selected from the group consisting of heart failure, pulmonary hypertension, and angina in a subject in need thereof comprising administering to the subject a therapeutically effective amount of the composition of Claim DATED this 1 st day of April 2004 BALDWIN SHELSTON WATERS Attorneys for: Ortho-McNeil Pharmaceutical, Inc.