AU2003304086A1 - Context sensitive parallel optimization of zinc finger dna binding domains - Google Patents

Context sensitive parallel optimization of zinc finger dna binding domains

Info

Publication number
AU2003304086A1
AU2003304086A1 AU2003304086A AU2003304086A AU2003304086A1 AU 2003304086 A1 AU2003304086 A1 AU 2003304086A1 AU 2003304086 A AU2003304086 A AU 2003304086A AU 2003304086 A AU2003304086 A AU 2003304086A AU 2003304086 A1 AU2003304086 A1 AU 2003304086A1
Authority
AU
Australia
Prior art keywords
dna binding
zinc finger
binding domains
context sensitive
finger dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003304086A
Other versions
AU2003304086A8 (en
Inventor
Keith J. Joung
Carl Pabo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Massachusetts Institute of Technology
Original Assignee
General Hospital Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Hospital Corp filed Critical General Hospital Corp
Publication of AU2003304086A1 publication Critical patent/AU2003304086A1/en
Publication of AU2003304086A8 publication Critical patent/AU2003304086A8/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2003304086A 2002-10-23 2003-10-23 Context sensitive parallel optimization of zinc finger dna binding domains Abandoned AU2003304086A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US42045802P 2002-10-23 2002-10-23
US60/420,485 2002-10-23
US46688903P 2003-04-30 2003-04-30
US60/466,889 2003-04-30
PCT/US2003/034010 WO2004099366A2 (en) 2002-10-23 2003-10-23 Context sensitive parallel optimization of zinc finger dna binding domains

Publications (2)

Publication Number Publication Date
AU2003304086A1 true AU2003304086A1 (en) 2004-11-26
AU2003304086A8 AU2003304086A8 (en) 2004-11-26

Family

ID=33436673

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003304086A Abandoned AU2003304086A1 (en) 2002-10-23 2003-10-23 Context sensitive parallel optimization of zinc finger dna binding domains

Country Status (2)

Country Link
AU (1) AU2003304086A1 (en)
WO (1) WO2004099366A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3461896B1 (en) 2011-07-15 2023-11-29 The General Hospital Corporation Methods of transcription activator like effector assembly
US9890364B2 (en) 2012-05-29 2018-02-13 The General Hospital Corporation TAL-Tet1 fusion proteins and methods of use thereof
EP2906602B1 (en) 2012-10-12 2019-01-16 The General Hospital Corporation Transcription activator-like effector (tale) - lysine-specific demethylase 1 (lsd1) fusion proteins
JP6445462B2 (en) 2013-02-07 2018-12-26 ザ ジェネラル ホスピタル コーポレイション TALE transcription activator
US10760064B2 (en) 2013-03-15 2020-09-01 The General Hospital Corporation RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci
CN110540991B (en) 2013-03-15 2023-10-24 通用医疗公司 Enhancement of specificity of RNA-guided genome editing using truncated guide RNA (tru-gRNA)
WO2014204578A1 (en) 2013-06-21 2014-12-24 The General Hospital Corporation Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing
AU2014346559B2 (en) 2013-11-07 2020-07-09 Editas Medicine,Inc. CRISPR-related methods and compositions with governing gRNAs
AU2015280069B2 (en) 2014-06-23 2021-08-12 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
WO2016054326A1 (en) 2014-10-01 2016-04-07 The General Hospital Corporation Methods for increasing efficiency of nuclease-induced homology-directed repair
US20180296537A1 (en) 2015-06-05 2018-10-18 Novartis Ag Methods and compositions for diagnosing, treating, and monitoring treatment of shank3 deficiency associated disorders
US9926546B2 (en) 2015-08-28 2018-03-27 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
US9512446B1 (en) 2015-08-28 2016-12-06 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
AU2016319110B2 (en) 2015-09-11 2022-01-27 The General Hospital Corporation Full interrogation of nuclease DSBs and sequencing (FIND-seq)
JP2018529353A (en) 2015-09-30 2018-10-11 ザ ジェネラル ホスピタル コーポレイション Comprehensive in vitro reporting of cleavage events by sequencing (CIRCLE-seq)
EP3525832A4 (en) 2016-10-14 2020-04-29 The General Hospital Corp. Epigenetically regulated site-specific nucleases
JP2020521446A (en) 2017-05-25 2020-07-27 ザ ジェネラル ホスピタル コーポレイション Bipartite base editor (BBE) structure and type II-C-CAS9 zinc finger editing
US11946040B2 (en) 2019-02-04 2024-04-02 The General Hospital Corporation Adenine DNA base editor variants with reduced off-target RNA editing
LT3812472T (en) 2019-10-21 2023-03-10 Albert-Ludwigs-Universität Freiburg A truly unbiased in vitro assay to profile off-target activity of one or more target-specific programmable nucleases in cells (abnoba-seq)

Also Published As

Publication number Publication date
AU2003304086A8 (en) 2004-11-26
WO2004099366A2 (en) 2004-11-18
WO2004099366A3 (en) 2006-07-20

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase
TH Corrigenda

Free format text: IN VOL 19, NO 1, PAGE(S) 44 UNDER THE HEADING APPLICATIONS OPI - NAME INDEX UNDER THE NAME THE GENERAL HOSPITAL CORPORATION, APPLICATION NO. 2003304086, UNDER INID (71) CORRECT THE NAME TO READ THE GENERAL HOSPITAL CORPORATION; MASSACHUSSETTS INSTITUTE OF TECHNLOGY