AU2003285007A1 - Kinase inhibitors - Google Patents

Description

WO 2004/041164 PCT/US2003/034100 TITLE OF THE INVENTION KINASE INHIBITORS BACKGROUND OF THE INVENTION 5 The present invention relates to compounds which inhibit, regulate and/or modulate kinase signal transduction, compositions which contain these compounds, and methods of using them to treat kinase-dependent diseases and conditions, such as angiogenesis, cancer, tumor growth, atherosclerosis, age related macular degeneration, diabetic retinopathy, retinal ischemia, macular edema, inflammatory diseases, and the like in mammals. 10 Kinases can be split into two main groups, tyrosine kinases such as KDR or Flk-1 and serine/threonine kinases such as cyclin dependent kinases or Cdk. Tyrosine kinases are a class of enzymes that catalyze the transfer of the terminal phosphate of adenosine triphosphate to tyrosine residues in protein substrates. Tyrosine kinases are believed, by way of substrate phosphorylation, to play critical roles in signal 15 transduction for a number of cell functions. Though the exact mechanism of signal transduction is still unclear, tyrosine kinases have been shown to be important contributing factors in cell proliferation, carcinogenesis, cell differentiation and apoptosis. Tyrosine kinases can be categorized as receptor type or non-receptor type. Receptor type tyrosine kinases have an extracellular, a transmembrane, and an intracellular 20 portion, while non-receptor type tyrosine kinases are wholly intracellular. The receptor type tyrosine kinases are comprised of a large number of transmembrane receptors with diverse biological activity. In fact, about twenty different subfamilies of receptor type tyrosine kinases have been identified. One tyrosine kinase subfamily, designated the HER subfamily, is comprised of EGFR, HER2, HER3, and HER4. 25 Ligands of this subfamily of receptors include epithileal growth factor, TGF-a, amphiregulin, HB-EGF, betacellulin and heregulin. Another subfamily of these receptor type tyrosine kinases is the insulin subfamily, which includes INS-R, IGF-IR, and IR-R. The PDGF subfamily includes the PDGF-a and -[ receptors, CSFIR, c-kit and FLK-IL Then there is the FLK family which is comprised of the kinase insert domain receptor (KDR), fetal liver kinase-1 (FLK-1), 30 fetal liver kinase-4 (FLK-4) and the fis-like tyrosine kinase-1 (flt-1). The PDGF and FLK families are usually considered together due to the similarities of the two groups. For a detailed discussion of the receptor type tyrosine kinases, see Plowman et al., DN&P 7(6):334 339 (1994), which is hereby incorporated by reference. The non-receptor type of tyrosine kinases is also comprised of numerous 35 subfamilies, including Src, Frk, Btk, Csk, Abl, Zap70, Fes/Fps, Fak, Jak, Ack, and LIMK. Each - 1- WO 2004/041164 PCT/US2003/034100 of these subfamilies is further sub-divided into varying receptors. For example, the Src subfamily is one of the largest and includes Src, Yes, Fyn, Lyn, Lck, Blk, Hek, Fgr, and Yrk. The Src subfamily of enzymes has been linked to oncogenesis. For a more detailed discussion of the non-receptor type of tyrosine kinases, see Bolen, Oncogene 8:2025-2031 (1993), which is 5 hereby incorporated by reference. Both receptor type and non-receptor type tyrosine kinases are implicated in cellular signaling pathways leading to numerous pathogenic conditions, including cancer, psoriasis and hyperimmune responses. Several receptor type tyrosine kinases, and the growth factors that bind thereto, 10 have been suggested to play a role in angiogenesis, although some may promote angiogenesis indirectly Mustonen and Alitalo, J. Cell Biol. 129:895-898 (1995). One such receptor type tyrosine kinase is fetal liver kinase 1 or FLK-1. The human analog of FLK-1 is the kinase insert domain-containing receptor KDR, which is also known as vascular endothelial cell growth factor receptor 2 or VEGFR-2, since it binds VEGF with high affinity. Finally, the murine version of 15 this receptor has also been called NYK. Oelrichs et al., Oncogene 8(1):11-15 (1993). VEGF and KDR are a ligand-receptor pair that play an important role in the proliferation of vascular endothelial cells, and the formation and sprouting of blood vessels, termed vasculogenesis and angiogenesis, respectively. Angiogenesis is characterized by excessive activity of vascular endothelial growth 20 factor (VEGF). VEGF is actually comprised of a family of ligands. Klagsburn and D'Amore, Cytokine &Growth Factor Reviews 7:259-270 (1996). VEGF binds the high affinity membrane spanning tyrosine kinase receptor KDR and the related fms-like tyrosine kinase-1, also known as Flt-1 or vascular endothelial cell growth factor receptor 1 (VEGFR-1). Cell culture and gene knockout experiments indicate that each receptor contributes to different aspects of angiogenesis. 25 KDR mediates the mitogenic function of VEGF whereas Flt-1 appears to modulate non mitogenic functions such as those associated with cellular adhesion. Inhibiting KDR thus modulates the level of mitogenic VEGF activity. In fact, tumor growth has been shown to be susceptible to the antiangiogenic effects of VEGF receptor antagonists. Kim et al., Nature 362:841-844 (1993). 30 Solid tumors can therefore be treated by tyrosine kinase inhibitors since these tumors depend on angiogenesis for the formation of the blood vessels necessary to support their growth. These solid tumors include histiocytic lymphoma, cancers of the brain, genitourinary tract, lymphatic system, stomach, larynx and lung, including lung adenocarcinoma and small cell lung cancer. Additional examples include cancers in which overexpression or activation of Raf 35 activating oncogenes (e.g., K-ras, erb-B) is observed. Such cancers include pancreatic and breast -2- WO 2004/041164 PCTIUS2003/034100 carcinoma. Accordingly, inhibitors of these tyrosine kinases are useful for the prevention and treatment of proliferative diseases dependent on these enzymes. The angiogenic activity of VEGF is not limited to tumors. VEGF accounts for most of the angiogenic activity produced in or near the retina in diabetic retinopathy. This 5 vascular growth in the retina leads to visual degeneration culminating in blindness. Ocular VEGF mRNA and protein are elevated by conditions such as retinal vein occlusion in primates and decreased P 0 2 levels in mice that lead to neovascularization. Intraocular injections of anti VEGF monoclonal antibodies or VEGF receptor immunofusions inhibit ocular neovascularization in both primate and rodent models. Regardless of the cause of induction of 10 VEGF in human diabetic retinopathy, inhibition of ocular VEGF is useful in treating the disease. Expression of VEGF is also significantly increased in hypoxic regions of animal and human tumors adjacent to areas of necrosis. VEGF is also upregulated by the expression of the oncogenes ras, raf, src and mutant p5 3 (all of which are relevant to targeting cancer). Monoclonal anti-VEGF antibodies inhibit the growth of human tumors in nude mice. Although 15 these same tumor cells continue to express VEGF in culture, the antibodies do not diminish their mitotic rate. Thus tumor-derived VEGF does not function as an autocrine mitogenic factor. Therefore, VEGF contributes to tumor growth in vivo by promoting angiogenesis through its paracrine vascular endothelial cell chemotactic and mitogenic activities. These monoclonal antibodies also inhibit the growth of typically less well-vascularized human colon cancers in 20 athymic mice and decrease the number of tumors arising from inoculated cells. Viral expression of VEGF-binding constructs of Flk-1 or Flt-1 (the mouse KDR receptor homologue), truncated to eliminate the cytoplasmic tyrosine kinase domains but retain the membrane anchors, virtually abolishes the growth of a transplantable glioblastoma in mice. Tumor growth is abolished presumably by a dominant negative mechanism during VEGF 25 receptor homodimerization. Embryonic stem cells, which normally grow as solid tumors in nude mice, do not produce detectable tumors if both VEGF alleles are knocked out. Taken together, these data indicate the role of VEGF in the growth of solid tumors. Inhibition of KDR or Flt-1 is implicated in pathological angiogenesis, and these receptors are useful in the treatment of diseases in which angiogenesis is part of the overall pathology, e.g., inflammation, diabetic 30 retinal vascularization, as well as various forms of cancer since tumor growth is known to be dependent on angiogenesis. Weidner et al., N. Engl. J. Med. 324:1-8 (1991). The cyclin-dependent protein kinases are regulators of the timing and coordination of eukaryotic cell cycle events. Norbury, C., and Nurse, P. (1992) Annu. Rev. Biochen. 61, 441-470, Sher, C.J. (1996) Science 274, 1672-1677. As such, cyclin dependent 35 kinases, their regulators and substrates are the targets of genetic alterations in many human -3- WO 2004/041164 PCT/US2003/034100 cancers. Kamb, A. et al. (1994) Science 264, 436-440, Nobori, et al. (1994) Nature 368, 753 756, Spruck, C.H. et al. Nature 370, 183-184, Hunter, T. and Pines, J. (1991) Cell 66, 1071 1074, Keyomarsi, K. and Pardee, A.B. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 1112-1116 and Wang, T.C. (1994) Nature 369, 669-67 1. 5 Members of the cyclin dependent kinase family include Cdk2 and Cdk4. Both are active in the G1 phase of cell cycle and regulate entry into the Gi/S phase transition. In one pathway, these kinases regulate the phosphorylation of the retinoblastoma protein. Substrate phosphorylation releases the E2F transcription factor which in turn regulates the expression of genes required for S phase entry. Inhibition of these kinases, therefore, blocks cell entry into the 10 S phase and downstream proliferative events. Small molecular cyclin dependent kinase inhibitors have already been identified and shown to have growth inhibitory activity against a number of different tumor types in vitro and in vivo. Glab, N. et al. (1994) FEBS Lett. 353, 207-211, Kitagawa, M. et al. (1993) Oncogene 8, 2425-2432, Losiewicz, M.D. et al. (1994) Biochem. Biophys. Res. Commun. 201, 15 589-595, Carlson, B.A. et al. (1996) Cancer Res. 56, 2973-2978, Kelland, L.R. (2000) Expert Opin. Invest. Drugs 9, 2903-2911 and Senderowicz, A.M. (1999) Invest. New Drugs 17, 313 320. Accordingly, the identification of small compounds which specifically inhibit, regulate and/or modulate the signal transduction of kinases is desirable and is an object of this 20 invention. SUMMARY OF THE INVENTION The present invention relates to compounds that are capable of inhibiting, modulating and/or regulating signal transduction of kinases. One embodiment of the present 25 invention is illustrated by a compound of Formula I, and the pharmaceutically acceptable salts and stereoisomers thereof:

(CH

2 )m

(CH

2 )n NN I \

R

2 4 N N S R 13 R -4- WO 2004/041164 PCT/US2003/034100 DETAILED DESCRIPTION OF THE INVENTION The compounds of this invention are useful in the inhibition of kinases and are illustrated by a compound of Formula I: R5 X

(CH

2 )m

(CH

2 )n R N N 4 XN N S R 13 R 5 or a pharmaceutically acceptable salt or stereoisomer thereof, wherein Xis 0, S or NR 3 ; m is 0, 1, 2 or 3; 10 n is 0, 1, 2 or 3; RI is: 1) H, 15 2) Or(C1-C6)perfluoroalkyl, 3) OH, 4) CN, 5) halogen, 6) (C= 0 )rOs(C1-C1o)alkyl, 20 7) (C=O)rOs(C2-C10)alkenyl, 8) (C=0)rOs(C2-C10)alkynyl, 9) (C=O)rOsaryl, 10) (C=0)rOsheterocyclyl, or 11) (CO-C6)alkyl-NRaRb, 25 wherein r and s are independently 0 or 1, and said alkyl, alkenyl, alkynyl, aryl and heterocyclyl is optionally substituted with one or more substituents selected from R 6 ; - 5- WO 2004/041164 PCT/US2003/034100

R

2 is: 1) H, 2) Or(C1-C6)perfluoroalkyl, 3) OH, 5 4) CN, 5) halogen, 6) (C=O)rOs(C1-C1O)alkyl, 7) (C=0)rOs(C2-C1O)alkenyl, 8) (C=O)rOs(C2-C1O)alkynyl, 10 9) (C=O)rOsaryl, 10) (C=0)rOsheterocyclyl, or 11) (CO-C6)alkyl-NRaRb, wherein r and s are independently 0 or 1, and said alkyl, alkenyl, alkynyl, aryl and heterocyclyl is optionally substituted with one or more substituents selected from R 6 ; 15

R

3 is: 1) H, 2)

SO

2 RC, 3) (C=O)rRc, wherein r is 0 or 1, or 20 4) CO2Rc;

R

4 is: 1) H, 2) Or(Ci-C6)perfluoroalkyl, 25 3) OH, 4) CN, 5) halogen, 6) (C=O)rOs(C1-C1O)alkyl, 7) (C=O)rOs(C2-C1O)alkenyl, 30 8) (C=O)rOs(C2-C1O)alkynyl, 9) (C=O)rOsaryl, 10) (C=O)rOsheterocyclyl, or 11) (CO-C6)alkyl-NRaRb, wherein r and s are independently 0 or 1, and said alkyl, alkenyl, alkynyl, aryl and heterocyclyl is 35 optionally substituted with one or more substituents selected from R 6 ; -6- WO 2004/041164 PCT/US2003/034100

R

5 is heterocyclyl wherein said heterocyclyl contains one or two heteroatoms selected from N, 0 and S, and is optionally substituted with one or more substituents selected from R 6 ; 5 R 6 is: 1) Or(C=O)sNRaRb, 2) (C=O)rOsaryl, 3) (C=0)rOs-heterocyclyl, 4) halogen, 10 5) OH, 6) oxo, 7) O(Ci-C3)perfluoroalkyl, 8) (C1 -C3)perfluoroalkyl, 9) (C=O)rOs(C1-C1O)alkyl, 15 10) CHO, 11) CO2H, or 12) CN, wherein r and s are independently 0 or 1, and said alkyl, aryl and heterocyclyl are optionally substituted with one or more substituents selected from Rd; 20 Ra and Rb are independently: 1) H, 2) (C=O)r(Ci-CIO)alkyl, 3) S(O) 2 Rc, 25 4) (C=O)rheterocyclyl, 5) (C=O)raryl, or 6) CO2Rc, wherein r is 0 or 1 and said alkyl, heterocyclyl, and aryl optionally substituted with one or more substituents selected from Rd, or 30 Ra and Rb are taken together with the nitrogen to which they are attached to form a monocyclic or bicyclic heterocycle with 5-7 members in each ring and optionally containing, in addition to the nitrogen, one or two additional heteroatoms selected from N, 0 and S, said monocyclic or bicyclic heterocycle optionally substituted with one or more substituents selected from Rd; 35 -7- WO 2004/041164 PCT/US2003/034100 RC is (C1-C6)alkyl, aryl, benzyl, or heterocyclyl; Rd is: 1) (C=0)rOs(C-C1O)alkyl, wherein r and s are independently 0 or 1, 5 optionally substituted with up to three substituents selected from OH, (C1 C6)alkoxy, halogen, CN, oxo, N(Re)2 and S(O)2Rc, 2) (C=O)N(Re) 2 , 3) Or(C1-C3)perfluoroalkyl, 4) (CO-C6)alkylene-S(O)mRc, wherein m is 0, 1, or 2, 10 5) oxo, 6) OH, 7) halogen, 8) CN, 9) (CO-C6)alkylene-aryl, optionally substituted with up to three substituents 15 selected from Re, 10) (CO-C6)alkylene-heterocyclyl, optionally substituted with up to three substituents selected from Re, 11) (C0-C6)alkylene-N(Re)2, 12) C(O)Rc, 20 13) CO2RC, 14) C(O)H, or 15) CO2H; and Re is H, (Cl-C6)alkyl, aryl, heterocyclyl or S(O)2Rc. 25 A further embodiment is illustrated by a compound as described directly above of Formula I, wherein R1 is H, CN, halogen, OH, (C=O)rOs(Ci-C1O) alkyl and (C=O)rOs(C1

C

10 )alkyl-NRaRb. A further embodiment is illustrated by a compound as described directly above of 30 Formula I, wherein R 2 is H, CN, OH, halogen, phenyl, wherein said phenyl is optionally substituted with one or more substituents selected from R 6 , (C=O)rOs(C1-C1O)alkyl and (C=O)rOs(C1-C10)alkyl-NRaRb. A further embodiment is illustrated by a compound as described directly above of Formula I, wherein R 4 is H, CN, halogen, phenyl, (C1-C6)alkyl, (C1-C6)perfluoroalkyl and 35 (C=O)rOsheterocyclyl. -8- WO 2004/041164 PCT/US2003/034100 Another embodiment is illustrated by a compound of Formula I described directly above wherein R1 is H; R 2 is CN or phenyl, R 3 is H, and R 4 is H or (C1-C6)alkyl. A preferred embodiment is a compound selected from: tert-butyl-4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]pyrimidin-4-yl oxy)piperidine 5 1-carboxylate; 2-{ [6-(piperidin-4-yloxy)pyrimidin-4-yl]amino 1-1,3-thiazole-5-carbonitrile; tert-butyl-4-({6-[5-phenyl-1,3-thiazol-2-yl)amino]pyrimidin-4-yl}oxy)piperidine 1-carboxylate; N-(5-phenyl-1,3-thiazol-2-yl)-6-(piperidin-4-yloxy)pyrimidin-4-amine; 10 tert-butyl-4-[({6-[5-cyano-1,3-thiazol-2-yl)amino]pyrimidin-4-yl oxy)methyl]-piperidine-1 carboxylate; tert-butyl-4-[({6-[(5-phenyl-1,3-thiazol-2-yl)amino]pyrimidin-4-yl oxy)methyl]-piperidine-1 carboxylate; N-(5-phenyl-1,3-thiazol-2-yl)-6-(piperidin-4-ylmethoxy)pyrimidin-4-amine; 15 2-{ [2-methyl-6-(piperidin-4-yloxy)pyrimidin-4-yl] amino }-1,3-thiazole-5-carbonitrile; N-(5-phenyl-1,3-thiazol-2-yl)-6-(piperidin-4-yloxy)-2-methylpyrimidin-4-amine; 2-({ 2-methyl-6-[(3R)-pyrrolidin-3-yloxy]pyrimidin-4-yl } amino)-1,3-thiazole-5-carbonitrile; 2-({2-methyl-6-[(3S)-pyrrolidin-3-yloxy]pyriniidin-4-yli amino)-1,3-thiazole-5-carbonitrile; 2-[2-methyl-6-{[1-(2-morpholin-4-ylethyl)piperidin-4-yl]oxy}pyrimidin-4-yl)amino]-1,3 20 thiazole-5-carbonitrile; 2-[4-({6-[5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-yl oxy)piperidin-1-yl]-N isopropylacetamide; 2- { [2-methyl-6-(3-morpholin-4-ylpropoxy)pyrimidin-4-yl] amino }-1,3-thiazole-5-carbonitrile; 2-{ [2-methyl-6-(2-morpholin-4-ylethoxy)pyrimidin-4-yl]amino}-1,3-thiazole-5-carbonitrile; 25 2-f [2-methyl-6-(2-piperidin-1 -ylethoxy)pyrimidin-4-yll amino }-1,3-thiazole-5-carbonitrile; 2-({ 2-methyl-6-[(2-morpholin-4-ylethyl)amino]pyrimidin-4-y } amino)- 1,3-thiazole 5-carbonitrile; 2-{ [6-(piperidin-4-ylmethoxy)pyrimidin-4-yl]amino 1-1,3-thiazole-5-carbonitrile; 2-f [2-methyl-6-(piperidin-4-ylmethoxy)pyrimidin-4-yl] amino }-1,3-thiazole-5-carbonitrile; 30 2-({6-[(3-morpholin-4-ylpropyl)amino]pyrimidin-4-yl } amino)- 1,3-thiazole-5-carbonitrile; 2-{ [2-methyl-6-(tetrahydro-2H-pyran-4-ylamino)pyrimidin-4-yl]amino I - 1,3-thiazole-5 carbonitrile; 2-[(6-{[3-(1H-imidazol-1-yl)propyl]amino}-2-methylpyrimidin-4-yl)amino]-1,3-thiazole-5 carbonitrile; -9- WO 2004/041164 PCT/US2003/034100 2- [(6-{ [(1,1 -dioxidotetrahyrothien-3ylmethyl] amino I -2-methylpyrimidin-4-yl) amino]-1,3 thiazole-5-carbonitrile; 2-({6-[(1,4-dioxan-2-ylmethyl)amino]-2-methylpyrimidin-4-y I amino)-1,3-thiazole-5 carbonitrile; 5 2-({6-[(3-morpholin-4-ylpropyl)amino]pyrimidin-4-yl I amino)-1,3-thiazole-5-carbonitrile; 2-[-({6-[5-cyano-1,3-thiazol-2-ylamino]-2-methylpyrimidin-4-yl } amino)piperidin-1-yl]-N isopropylacetamide; tert-butyl-4-({6-[(5-cyano-1,3-thiazol-2-ylamino]-2-methylpyrimidin-4-yl } amino) piperidine-1 carboxylate; 10 2-{ [2-methyl-6-(piperidin-4-ylamino)pyrimidin-4-yl]amino}-1,3-thiazole-5-carbonitrile; tert-butyl-4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]methyl }-2-methylpyrimidin-4-yl } amino) piperidine-1-carboxylate; 2-({2-methyl-6-[(piperidin-4-ylmethyl)amino]pyrimidin-4-yl I amino)-1,3-thiazole-5-carbonitrile; 2-{[5-methyl-6-(piperidin-4-ylamino)pyrimidin-4-yl]oxy}-1,3-thiazole-5-carbonitrile; 15 tert-butyl-2-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-yl oxy) methyl] morpholine-4-carboxylate; 2-'{ [2-methyl-6-(morpholin-2-ylmethoxy)pyrimidin-4-yl] amino}-1,3-thiazole-5-carbonitrile; 2-1[2-methyl-6-(tetrahydro-2-pyran-4-yloxy)pyrimidin-4-yl]amino}-1,3-thiazole-5-carbonitrile; 2-{ [2-isopropyl-6-(piperidin-4-yloxy)pyrimidin-4-yl]amino }-1,3-thiazole-5-carbonitrile; 20 2-({6-[(1, 1-dioxidotetrahydrothien-3-yl)amino]-2-methylpyrimidin-4-y I amino)- 1,3-thiazole-5 carbonitrile; 2-{ [2-methyl-6-(tetrahydrofuran-3-ylamino)pyrimidin-4-yl] amino }-1,3-thiazole-5-carbonitrile; tert-butyl{4-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-yl} oxy) methyl]piperidin- 1-yl }acetate; 25 {4-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-yl}oxy)methyl] piperidin-1 yl} acetic acid; N-(tert-butyl)-2-{4-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-ylI oxy)methyl]piperidin-1-yl acetamide; 2-({2-methyl-6-[(2-morpholin-4-ylethyl)thio]pyrimidin-4-yl I amino)- 1,3-thiazole-5-carbonitrile; 30 and 2-{ [6-(piperidin-4-ylthio)pyrimidin-4-yl]amino }- 1,3-thiazole-5-carbonitrile; or a pharmaceutically acceptable salt or stereoisomer thereof. Yet another embodiment of the present invention is a compound which is: 2-({2 methyl-6-[(3S)-pyrrolidin-3-yloxy]pyrimidin-4-yl } amino)- 1,3-thiazole-5-carbonitrile -10- WO 2004/041164 PCT/US2003/034100 HN ),11 N ,"ON N CN H or a pharmaceutically acceptable salt or stereoisomer thereof. Another embodiment of the present invention is a compound which is: N-(5-phenyl-1,3-thiazol-2-yl)-6-(piperidin-4-yloxy)pyrimidin-4-amine 5 0 HN N N N H S or a pharmaceutically acceptable salt thereof. Another embodiment of the present invention is a compound which is2-{[2 methyl-6-(piperidin-4-yloxy)pyrimidin-4-yl] amino 1-1,3-thiazole-5-carbonitrile HN N C 10 H or a pharmaceutically acceptable salt thereof. And yet another embodiment of the present invention is a compound which is 2 { [2-methyl-6-(morpholin-2-ylmethoxy)pyrimidin-4-yl]amino }-1 ,3-thiazole-5-carbonitrile - 11 - WO 2004/041164 PCT/US2003/034100 H N O O N '111, / CN N N S H or a pharmaceutically acceptable salt or stereoisomer thereof. Another embodiment of the present invention is a compound which is 2-({6-[(1,1-dioxidotetrahydrothien-3-yl)aminol-2-methylpyrimidin-4-yl}amino)-1,3-thiazole-5 5 carbonitrile S NH N N N N S CN H or a pharmaceutically acceptable salt or stereoisomer thereof. In yet another embodiment of the present invention is a compound which is 2-{[2 methyl-6-(tetrahydrofuran-3-ylamino)pyrimidin-4-yl]amino }-1,3-thiazole-5-carbonitrile 10 0 NH N '~ N NC N N CN H or a pharmaceutically acceptable salt or stereoisomer thereof. Another embodiment of the present invention is a compound which is 2-{[2 isopropyl-6-(piperidin-4-yloxy)pyriniidin-4-yl]amino}-1,3-thiazole-5-carbonitrile - 12 - WO 2004/041164 PCT/US2003/034100 HNaO NN N CN or a pharmaceutically acceptable salt thereof. Also included within the scope of the present invention is a pharmaceutical composition which is comprised of a compound of Formula I as described above and a 5 pharmaceutically acceptable carrier. The invention is also contemplated to encompass a pharmaceutical composition which is comprised of a pharmaceutically acceptable carrier and any of the compounds specifically disclosed in the present application. These and other aspects of the present invention will be apparent from the teachings contained herein. 10 Utilities The compounds of the present invention are inhibitors of kinases and are therefore useful to treat or prevent kinase-dependent diseases or conditions in mammals. "Tyrosine kinase-dependent diseases or conditions" refers to pathologic conditions that depend on the activity of one or more tyrosine kinases. Tyrosine kinases either 15 directly or indirectly participate in the signal transduction pathways of a variety of cellular activities including proliferation, adhesion and migration, and differentiation. Diseases associated with tyrosine kinase activities include the proliferation of tumor cells, the pathologic neovascularization that supports solid tumor growth, ocular neovascularization (diabetic retinopathy, age-related macular degeneration, retinal ischemia, macular edema and the like) and 20 inflammation (psoriasis, rheumatoid arthritis, and the like). In treating such conditions with the instantly claimed compounds, the required therapeutic amount will vary according to the specific disease and is readily ascertainable by those skilled in the art. Although both treatment and prevention are contemplated by the scope of the invention, the treatment of these conditions is the preferred use. 25 The present invention encompasses a method of treating or preventing cancer in a mammal in need of such treatment which is comprised of administering to said mammal a therapeutically effective amount of a claimed compound. Preferred cancers for treatment are selected from cancers of the brain, genitourinary tract, lymphatic system, stomach, larynx and lung. Another set of preferred forms of cancer are histiocytic lymphoma, lung adenocarcinoma, - 13 - WO 2004/041164 PCT/US2003/034100 small cell lung cancers, pancreatic cancer, glioblastomas and breast carcinoma. A further preferred group of cancers for treatment with the present compounds is a cancer selected from lung cancer, prostate cancer, breast cancer and colorectal cancer. The utility of angiogenesis inhibitors in the treatment of cancer is known in the literature, see J. Rak et al. Cancer Research, 5 55:4575-4580, 1995, for example. The role of angiogenesis in cancer has been shown in numerous types of cancer and tissues: breast carcinoma (G. Gasparini and A.L. Harris, J. Clin. Oncol., 1995, 13:765-782; M. Toi et al., Japan. J. Cancer Res., 1994, 85:1045-1049); bladder carcinomas (A.J. Dickinson et al., Br. J. Urol., 1994, 74:762-766); colon carcinomas (L.M. Ellis et al., Surgery, 1996, 120(5):871-878); and oral cavity tumors (J.K. Williams et al., Am. J. Surg., 10 1994, 168:373-380). Tumors which have undergone neovascularization show an increased potential for metastasis. VEGF released from cancer cells enhances metastasis possibly by increasing extravasation at points of adhesion to vascular endothelium. (A. Amirkhosravi et al., Platelets, 10:285-292 (1999)). In fact, angiogenesis is essential for tumor growth and metastasis. (S.P. 15 gunningham, et al., Can. Research, 61: 3206-3211 (2001)). The angiogenesis inhibitors disclosed in the present application are therefore also useful to prevent or decrease tumor cell metastasis. Such a use is also contemplated to be within the scope of the present invention. Further included within the scope of the invention is a method of treating or preventing a disease in which angiogenesis is implicated, which is comprised of administering to 20 a mammal in need of such treatment a therapeutically effective amount of a compound of the present invention. Ocular neovascular diseases are an example of conditions where much of the resulting tissue damage can be attributed to aberrant infiltration of blood vessels in the eye (see WO 00/30651, published 2 June 2000). The undesireable infiltration can be triggered by ischemic retinopathy, such as that resulting from diabetic retinopathy, retinopathy of 25 prematurity, retinal vein occlusions, etc., or by degenerative diseases, such as the choroidal neovascularization observed in age-related macular degeneration. Inhibiting the growth of blood vessels by administration of the present compounds would therefore prevent the infiltration of blood vessels and prevent or treat diseases where angiogenesis is implicated, such as ocular diseases like retinal vascularization, diabetic retinopathy, retinal ischemia, macular edema, age 30 related macular degeneration, and the like. Also included within the scope of the present invention is a method of treating or preventing inflammatory diseases which comprises administering to a mammal in need of such treatment a therapeutically effective amount of a compound of Formual I. Examples of such inflammatory diseases are rheumatoid arthritis, psoriasis, contact dermatitis, delayed 35 hypersensitivity reactions, and the like. (A. Giatromanolaki et al., J. Pathol. 2001; 194:101 - 14- WO 2004/041164 PCT/US2003/034100 108.) For the role of VEGF in skin angiogenesis, see Michael Detmar, J. Dermatological Sci., 24 Supply. 1, S78-S84 (2000). Also included within the scope of the present invention is a method of treating or preventing bone associated pathologies selected from osteosarcoma, osteoarthritis, and rickets, 5 also known as oncogenic osteomalacia. (Hasegawa et al., Skeletal Radiol., 28, pp.41-45, 1999; Gerber et al., Nature Medicine, Vol. 5, No. 6, pp.623-628, June 1999.) And since VEGF directly promotes osteoclastic bone resorption through KDR/Flk-1 expressed in mature osteoclasts (FEBS Let. 473:161-164 (2000); Endocrinology, 141:1667 (2000)), the instant compounds are also useful to treat and prevent conditions related to bone resorption, such as osteoporosis and 10 Paget's disease. A method of treating or preventing preeclampsia is also within the scope of the present invention, which comprises administering a therapeutically effective amount of a compound of Formula I. Studies have shown that the action of VEGF on the Flt-1 receptor is pivotal in the pathogenesis of preeclampsia. (Laboratory Investigation 79:1101-1111 15 (September 1999)). Vessels of pregnant women incubated with VEGF exhibit a reduction in endothelium-dependent relaxation similar to that induced by plasma from women with preeclampsia. In the presence of an anti-Flt-1 receptor antibody, however, neither ViEGF or plasma from women with preeclampsia reduced the endothelium-dependent relaxation. Therefore the claimed compounds serve to treat preeclampsia via their action on the tyrosine 20 kinase domain of the Flt-1 receptor. Also within the scope of the invention is a method of reducing or preventing tissue damage following a cerebral ischemic event which comprises administering a therapeutically effective amount of a compound of the present invention. The claimed compounds can also be used to reduce or prevent tissue damage which occurs after cerebral 25 ischemic events, such as stroke, by reducing cerebral edema, tissue damage, and reperfusion injury following ischemia. (Drug News Perspect 11:265-270 (1998); J. Clin. Invest. 104:1613 1620 (1999); Nature Med 7:222-227 (2001)). The instant compounds can also be used to prevent or treat tissue damage during bacterial meningitis, such as tuberculous meningitis. (Matsuyama et al., J. Neurol. Sci. 186:75 30 79 (2001)). The instant invention therefore encompasses a method of treating or preventing tissue damage due to bacterial meningitis which comprises administering a therapeutically effective amount of a claimed compound. Studies have shown that VEGF is secreted by inflammatory cells during bacterial meningitis and that VEGF contributes to blood-brain barrier disruption. (van der Flier et al., J. Infectious Diseases, 183:149-153 (2001)). The claimed - 15 - WO 2004/041164 PCT/US2003/034100 compounds can inhibit VEGF-induced vascular permeability and therefore serve to prevent or treat blood-brain barrier disruption associated with bacterial meningitis. The present invention further encompasses a method to treat or prevent endometriosis comprised of administering a therapeutically effective amount of a claimed 5 compound. An increase in VEGF expression and angiogenesis is associated with the progression of endometriosis (Stephen K. Smith, Trends in Endocrinology & Metabolism, Vol. 12, No. 4, May/June 2001). Inhibition of VEGF by the current compounds would therefore inhibit angiogenesis and treat endometriosis. A further embodiment of the present invention is a method of treating acute 10 myeloid leukemia (AML) which comprises administering a therapeutically effective amount of a claimed compound. Activation of FLT3 on leukemic cells by FLT3 ligand leads to receptor dimerization and signal transduction in pathways that promote cell growth and inhibit apoptosis (Blood, Vol. 98, No. 3, pp.885-887 (2001)). The present compounds are therefore useful to treat AML via inhibition of the tyrosine kinase domain of Flt-3. 15 Another embodiment of the present invention is a method of treating or preventing cancer via the dual inhibition of cyclin dependent kinase and tyrosine kinase. Cyclin dependent kinases are known to regulate cell cycle progression and cyclin dependent kinase inhibitors have been shown to block cell proliferation. As described above, inhibition of tyrosine kinases is useful in the treatment and prevention of cancer. Furthermore, inhibition of cyclin dependant 20 kinases is also useful in the treatment and prevention of cancer (Glab et al., FEBS Lett. 353, 207 211 (1994), Kitagawa et al., Oncogene. 8, 2425-2432 (1993), Losiewicz et al., Biochem. Biophys. Res. Commun. 201, 589-595 (1994), Carlson et al. Cancer Res. 56, 2973-2978 (1996), Kelland, L.R. Expert Opin. Invest. Drugs. 9, 2903-2911 (2000) and Senderowicz, A.M. Invest. New Drugs. 17, 313-320 (1999)). Thus, dual inhibition of two separate signaling pathways 25 provides an advantage in the inhibition of cell proliferation and cancer progression. The present compounds demonstrate dual inhibitory activity against cyclin dependent kinases as well as tyrosine kinases and are therefore useful in the treatment and prevention of cancer. The present invention encompasses a method of treating or preventing cancer via the dual inhibition of tyrosine kinase and cyclin dependent kinase wherein the dual inhibitor is 30 selected from: tert-butyl{4-[({ 6 -[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-yl oxy) methyl]piperidin-1-yl }acetate; 2-{ [2-methyl-6-(piperidin-4-yloxy)pyrimidin-4-yl]amino}-1,3-thiazole-5-carbonitrile; 2-{[ 2 -methyl-6-(piperidin-4-ylmethoxy)pyrimidin-4-yl]amino}-1,3-thiazole-5-carbonitrile; and 35 2-{[ 2 -isopropyl-6-(piperidin-4-yloxy)pyrimidin-4-yl]amino}-1,3-thiazole-5-carbonitrile; -16- WO 2004/041164 PCT/US2003/034100 or a pharmaceutically acceptable salt thereof. The compounds of this invention may be administered to mammals, preferably humans, either alone or, preferably, in combination with pharmaceutically acceptable carriers or diluents, optionally with known adjuvants, such as alum, in a pharmaceutical composition, 5 according to standard pharmaceutical practice. The compounds can be administered orally or parenterally, including the intravenous, intramuscular, intraperitoneal, subcutaneous, rectal and topical routes of administration. For oral use of a chemotherapeutic compound according to this invention, the selected compound may be administered, for example, in the form of tablets or capsules, or as an 10 aqueous solution or suspension. In the case of tablets for oral use, carriers which are commonly used include lactose and cornstarch, and lubricating agents, such as magnesium stearate, are commonly added. For oral administration in capsule form, useful diluents include lactose and dried corn starch. When aqueous suspensions are required for oral use, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening and/or 15 flavoring agents may be added. For intramuscular, intraperitoneal, subcutaneous and intravenous use, sterile solutions of the active ingredient are usually prepared, and the pH of the solutions should be suitably adjusted and buffered. For intravenous use, the total concentration of solutes should be controlled in order to render the preparation isotonic. The instant compounds are also useful in combination with known anti-cancer 20 agents. Combinations of the presently disclosed compounds with other anti-cancer or chemotherapeutic agents are within the scope of the invention. Examples of such agents can be found in Cancer Principles and Practice of Oncology by V.T. Devita and S. Hellman (editors), 6th edition (February 15, 2001), Lippincott Williams & Wilkins Publishers. A person of ordinary skill in the art would be able to discern which combinations of agents would be useful based on 25 the particular characteristics of the drugs and the cancer involved. Such anti-cancer agents include the following: estrogen receptor modulators, androgen receptor modulators, retinoid receptor modulators, cytotoxic agents, antiproliferative agents, prenyl-protein transferase inhibitors, HMG-CoA reductase inhibitors, HIV protease inhibitors, reverse transcriptase inhibitors, and other angiogenesis inhibitors. The instant compounds are particularly useful 30 when coadminsitered with radiation therapy. The synergistic effects of inhibiting VEGF in combination with radiation therapy have been described in the art (see WO 00/61186). The use of angiogenesis inhibitors with other chemotherapeutic agents is especially desirable since the normalization of tumor vasculature improves the delivery of the other therapeutic agents. (Nature Medicine, Vol. 7, No. 9, pp. 987-989 (September 2001)). - 17 - WO 2004/041164 PCT/US2003/034100 "Estrogen receptor modulators" refers to compounds which interfere or inhibit the binding of estrogen to the receptor, regardless of mechanism. Examples of estrogen receptor modulators include, but are not limited to, tamoxifen, raloxifene, idoxifene, LY3533 81, LY117081, toremifene, fulvestrant, 4-[7-(2,2-dimethyl-1-oxopropoxy-4-methyl-2-[4-[2-(1 5 piperidinyl)ethoxy]phenyl]-2H-1-benzopyran-3-yl]-phenyl-2,2-dimethylpropanoate, 4,4' dihydroxybenzophenone-2,4-dinitrophenyl-hydrazone, and SH646. "Androgen receptor modulators" refers to compounds which interfere or inhibit the binding of androgens to the receptor, regardless of mechanism. Examples of androgen receptor modulators include finasteride and other 5a-reductase inhibitors, nilutamide, flutamide, 10 bicalutamide, liarozole, and abiraterone acetate. For an example of a previously reported combination of an androgen receptor modulator (a non-steroidal anti-androgen, in this case) and a tyrosine kinase inhibitor, see WO 0176586, published on 18 October 2001. "Retinoid receptor modulators" refers to compounds which interfere or inhibit the binding of retinoids to the receptor, regardless of mechanism. Examples of such retinoid 15 receptor modulators include bexarotene, tretinoin, 13-cis-retinoic acid, 9-cis-retinoic acid, a difluoromethylornithine, ILX23-7553, trans-N-(4'-hydroxyphenyl) retinamide, and N-4 carboxyphenyl retinamide. "Cytotoxic agents" refer to compounds which cause cell death primarily by interfering directly with the cell's functioning or inhibit or interfere with cell myosis, including 20 alkylating agents, tumor necrosis factors, intercalators, microtubulin inhibitors, and topoisomerase inhibitors. Examples of cytotoxic agents include, but are not limited to, tirapazimine, sertenef, cachectin, ifosfamide, tasonermin, lonidamine, carboplatin, altretamine, prednimustine, dibromodulcitol, ranimustine, fotemustine, nedaplatin, oxaliplatin, temozolomide, heptaplatin, 25 estramustine, improsulfan tosilate, trofosfamide, nimustine, dibrospidium chloride, pumitepa, lobaplatin, satraplatin, profiromycin, cisplatin, irofulven, dexifosfamide, cis-aminedichloro(2 methyl-pyridine)platinum, benzylguanine, glufosfamide, GPX100, (trans, trans, trans)-bis-mu (hexane-1,6-diamine)-mu-[diamine-platinum(Il)]bis[diamine(chloro)platinum (II)]tetrachloride, diarizidinylspermine, arsenic trioxide, 1-(11-dodecylamino-10-hydroxyundecyl)-3,7 30 dimethylxanthine, zorubicin, idarubicin, daunorubicin, bisantrene, mitoxantrone, pirarubicin, pinafide, valrubicin, amrubicin, antineoplaston, 3'-deamino-3'-morpholino-13-deoxo-10 hydroxycarminomycin, annamycin, galarubicin, elinafide, MEN10755, and 4-demethoxy-3 deamino-3-aziridinyl-4-methylsulphonyl-daunorubicin (see WO 00/50032). Examples of microtubulin inhibitors include paclitaxel, vindesine sulfate, 3',4' 35 didehydro-4'-deoxy-8'-norvincaleukoblastine, docetaxol, rhizoxin, dolastatin, mivobulin - 18 - WO 2004/041164 PCT/US2003/034100 isethionate, auristatin, cemadotin, RPR109881, BMS184476, vinflunine, cryptophycin, 2,3,4,5,6-pentafluoro-N-(3-fluoro-4-methoxyphenyl) benzene sulfonamide, anhydrovinblastine, N,N-dimethyl-L-valyl-L-valyl-N-methyl-L-valyl-L-prolyl-L-proline-t-butylamide, TDX258, and BMS188797. 5 Some examples of topoisomerase inhibitors are topotecan, hycaptamine, irinotecan, rubitecan, 6-ethoxypropionyl-3',4'-O-exo-benzylidene-chartreusin, 9-methoxy-N,N dimethyl-5-nitropyrazolo[3,4,5-kl]acridine-2-(6H) propanamine, 1-amino-9-ethyl-5-fluoro-2,3 dihydro-9-hydroxy-4-methyl-1H, 1 2H-benzo [de]pyrano[3',4':b,7]indolizino[ 1,2b]quinoline 10,13(9H,15H)dione, lurtotecan, 7-[2-(N-isopropylamino)ethyl]-(20S)camptothecin, BNP1350, 10 BNPI1 100, BN80915, BN80942, etoposide phosphate, teniposide, sobuzoxane, 2' dimethylamino-2'-deoxy-etoposide, GL331, N-[2-(dimethylamino)ethyl]-9-hydroxy-5,6 dimethyl-6H-pyrido[4,3-b]carbazole-1-carboxamide, asulacrine, (5a, 5aB, 8aa,9b)-9-[2-[N-[2 (dimethylamino)ethyl]-N-methylamino]ethyl]-5-[4-hydroxy-3,5-dimethoxyphenyl] 5,5a,6,8,8a,9-hexohydrofuro(3',4':6,7)naphtho(2,3-d)-1,3-dioxol-6-one, 2,3-(methylenedioxy)-5 15 methyl-7-hydroxy-8-methoxybenzo[c]-phenanthridinium, 6,9-bis[(2 aminoethyl)amino]benzo[g]isoguinoline-5,10-dione, 5-(3-aminopropylamino)-7,10-dihydroxy-2 (2-hydroxyethylaminomethyl)-6H-pyrazolo[4,5,1-de]acridin-6-one, N-[1 [2(diethylamino)ethylamino]-7-methoxy-9-oxo-9H-thioxanthen-4-ylmethyl]formamide, N-(2 (dimethylamino)ethyl)acridine-4-carboxamide, 6-[[2-(dimethylamino)ethyl]amino]-3-hydroxy 20 7H-indeno[2,1-c] quinolin-7-one, and dimesna. "Antiproliferative agents" includes antisense RNA and DNA oligonucleotides such as G3139, ODN698, RVASKRAS, GEM231, and INX3001, and antimetabolites such as enocitabine, carmofur, tegafur, pentostatin, doxifluridine, trimetrexate, fludarabine, capecitabine, galocitabine, cytarabine ocfosfate, fosteabine sodium hydrate, raltitrexed, paltitrexid, emitefur, 25 tiazofurin, decitabine, nolatrexed, pemetrexed, nelzarabine, 2'-deoxy-2'-methylidenecytidine, 2' fluoromethylene-2'-deoxycytidine, N-[5-(2,3-dihydro-benzofuryl)sulfonyl]-N'-(3,4 dichlorophenyl)urea, N6-[4-deoxy-4-[N2-[2(E),4(E)-tetradecadienoyl]glycylamino]-L-glycero B-L-manno-heptopyranosyl]adenine, aplidine, ecteinascidin, troxacitabine, 4-[2-amino-4-oxo 4,6,7,8-tetrahydro-3H-pyrimidino[5,4-b][1,4]thiazin-6-yl-(S)-ethyl]-2,5-thienoyl-L-glutamic 30 acid, aminopterin, 5-flurouracil, alanosine, 11-acetyl-8-(carbamoyloxymethyl)-4-formyl-6 methoxy- 14-oxa-1,11 -diazatetracyclo(7.4. 1.0.0)-tetradeca-2,4,6-trien-9-y acetic acid ester, swainsonine, lometrexol, dexrazoxane, methioninase, 2'-cyano-2'-deoxy-N4-palmitoyl-1-B-D arabino furanosyl cytosine, and 3-aminopyridine-2-carboxaldehyde thiosemicarbazone. "Antiproliferative agents" also includes monoclonal antibodies to growth factors, other than 35 those listed under "angiogenesis inhibitors", such as trastuzumab, and tumor suppressor genes, -19- WO 2004/041164 PCT/US2003/034100 such as p53, which can be delivered via recombinant virus-mediated gene transfer (see U.S. Patent No. 6,069,134, for example). "HMG-CoA reductase inhibitors" refers to inhibitors of 3-hydroxy-3 methylglutaryl-CoA reductase. Compounds which have inhibitory activity for HMG-CoA 5 reductase can be readily identified by using assays well-known in the art. For example, see the assays described or cited in U.S. Patent 4,231,938 at col. 6, and WO 84/02131 at pp. 30-33. The terms "HMG-CoA reductase inhibitor" and "inhibitor of HMG-CoA reductase" have the same meaning when used herein. Examples of HMG-CoA reductase inhibitors that may be used include but are not 10 limited to lovastatin (MEVACOR@; see U.S. Patent Nos. 4,231,938, 4,294,926 and 4,319,039), simvastatin (ZOCOR@; see U.S. Patent Nos. 4,444,784, 4,820,850 and 4,916,239), pravastatin (PRAVACHOL@; see U.S. Patent Nos. 4,346,227, 4,537,859, 4,410,629, 5,030,447 and 5,180,589), fluvastatin (LESCOL®; see U.S. Patent Nos. 5,354,772, 4,911,165, 4,929,437, 5,189,164, 5,118,853, 5,290,946 and 5,356,896), atorvastatin (LIPITOR®; see U.S. Patent Nos. 15 5,273,995, 4,681,893, 5,489,691 and 5,342,952) and cerivastatin (also known as rivastatin and BAYCHOL@; see US Patent No. 5,177,080). The structural formulas of these and additional HMG-CoA reductase inhibitors that may be used in the instant methods are described at page 87 of M. Yalpani, "Cholesterol Lowering Drugs", Chemistry & Industry, pp. 85-89 (5 February 1996) and US Patent Nos. 4,782,084 and 4,885,314. The term HMG-CoA reductase inhibitor as 20 used herein includes all pharmaceutically acceptable lactone and open-acid forms (i.e., where the lactone ring is opened to form the free acid) as well as salt and ester forms of compounds which have HMG-CoA reductase inhibitory activity, and therefor the use of such salts, esters, open acid and lactone forms is included within the scope of this invention. An illustration of the lactone portion and its corresponding open-acid form is shown below as structures I and 11. HO O0 HO COOH 0 t,,OH Lactone Open-Acid 25 I H In HMG-CoA reductase inhibitors where an open-acid form can exist, salt and ester forms may preferably be formed from the open-acid, and all such forms are included within the meaning of the term "HMG-CoA reductase inhibitor" as used herein. Preferably, the HMG CoA reductase inhibitor is selected from lovastatin and simvastatin, and most preferably - 20 - WO 2004/041164 PCT/US2003/034100 simvastatin. Herein, the term "pharmaceutically acceptable salts" with respect to the HMG-CoA reductase inhibitor shall mean non-toxic salts of the compounds employed in this invention which are generally prepared by reacting the free acid with a suitable organic or inorganic base, particularly those formed from cations such as sodium, potassium, aluminum, calcium, lithium, 5 magnesium, zinc and tetramethylammonium, as well as those salts formed from amines such as ammonia, ethylenediamine, N-methylglucamine, lysine, arginine, ornithine, choline, N,N' dibenzylethylenediamine, chloroprocaine, diethanolamine, procaine, N-benzylphenethylamine, 1-p-chlorobenzyl-2-pyrrolidine-1'-yl-methylbenz-imidazole, diethylamine, piperazine, and tris(hydroxymethyl) aminomethane. Further examples of salt forms of HIMG-CoA reductase 10 inhibitors may include, but are not limited to, acetate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, calcium edetate, camsylate, carbonate, chloride, clavulanate, citrate, dihydrochloride, edetate, edisylate, estolate, esylate, fumarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynapthoate, iodide, isothionate, lactate, lactobionate, laurate, malate, 15 maleate, mandelate, mesylate, methylsulfate, mucate, napsylate, nitrate, oleate, oxalate, pamaote, palmitate, panthothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, subacetate, succinate, tannate, tartrate, teoclate, tosylate, triethiodide, and valerate. Ester derivatives of the described HMG-CoA reductase inhibitor compounds may act as prodrugs which, when absorbed into the bloodstream of a warm-blooded animal, may 20 cleave in such a manner as to release the drug form and permit the drug to afford improved therapeutic efficacy. "Prenyl-protein transferase inhibitor" refers to a compound which inhibits any one or any combination of the prenyl-protein transferase enzymes, including farnesyl-protein transferase (FPTase), geranylgeranyl-protein transferase type I (GGPTase-I), and geranylgeranyl 25 protein transferase type-II (GGPTase-II, also called Rab GGPTase). Examples of prenyl-protein transferase inhibiting compounds include (±)-6-[amino(4-chlorophenyl)(1-methyl-1H-imidazol 5-yl)methyl]-4-(3-chlorophenyl)-1-methyl-2(1H)-quinolinone, (-)-6-[amino(4-chlorophenyl)(1 methyl-1H-imidazol-5-yl)methyl]-4-(3-chlorophenyl)-1-methyl-2(1H)-quinolinone, (+)-6 [amino(4-chlorophenyl)(1-methyl-1H-imidazol-5-yl) methyl]-4-(3-chlorophenyl)-1-methyl 30 2(1H)-quinolinone, 5(S)-n-butyl-1-(2,3-dimethylphenyl)-4-[1-(4-cyanobenzyl)-5 imidazolylmethyl]-2-piperazinone, (S)-1-(3-chlorophenyl) -4-[1-(4-cyanobenzyl)-5 imidazolylmethyl]-5-[2-(ethanesulfonyl) methyl)-2-piperazinone, 5(S)-n-Butyl-1-(2 methylphenyl)-4-[1-(4-cyanobenzyl)-5-imidazolylmethyl]-2-piperazinone, 1-(3-chlorophenyl) 4 -[l-( 4 -cyanobenzyl)- 2 -methyl-5-imidazolylmethyl]-2-piperazinone, 1-(2,2-diphenylethyl)-3-[N 35 (1-(4-cyanobenzyl)-1H-imidazol-5-ylethyl)carbamoyl]piperidine, 4-{5-[4-hydroxymethyl-4-(4 - 21 - WO 2004/041164 PCT/US2003/034100 chloropyridin-2-ylmethyl)-piperidine-1-ylmethyl]-2-methylimidazol-1-ylmethyl} benzonitrile, 4 f5-[4-hydroxymethyl-4-(3-chlorobenzyl)-piperidine-1-ylmethyl]-2-methylimidazol-1 ylmethyl}benzonitrile, 4-{3-[4-(2-oxo-2H-pyridin-1-yl)benzyl]-3H-imidazol-4 ylmethyl}benzonitrile, 4-{3-[4-(5-chloro-2-oxo-2H-[1,2']bipyridin-5'-ylmethyl]-3H-imidazol-4 5 ylmethyl}benzonitrile, 4-{3-[4-(2-oxo-2H-[1,2'] bipyridin-5'-ylmethyl]-3H-imidazol-4 ylmethyl}benzonitrile, 4-[3-(2-oxo-1-phenyl-1,2-dihydropyridin-4-ylmethyl)-3H-imidazol-4 ylmethyl}benzonitrile, 18,19-dihydro-19-oxo-5H,17H-6,10:12,16-dimetheno-1H-imidazo[4,3 c][1,11,4]dioxaazacyclo-nonadecine-9-carbonitrile, (±)-19,20-dihydro-19-oxo-5H-18,21-ethano 12,14-etheno-6,10-metheno-22H-benzo[d]imidazo[4,3-k][1,6,9,12]oxatriaza-cyclooctadecine-9 10 carbonitrile, 19,20-dihydro-19-oxo-5H,17H-18,21-ethano-6,10:12,16-dimetheno-22H imidazo[3,4-h][1,8,11,14]oxatriazacycloeicosine-9-carbonitrile, and (±)-19,20-dihydro-3 methyl-19-oxo-5H-18,21-ethano-12,14-etheno-6,10-metheno-22H-benzo [d]imidazo[4,3 k][1,6,9,12]oxa-triazacyclooctadecine-9-carbonitrile. Other examples of prenyl-protein transferase inhibitors can be found in the 15 following publications and patents: WO 96/30343, WO 97/18813, WO 97/21701, WO 97/23478, WO 97/38665, WO 98/28980, WO 98/29119, WO 95/32987, U.S. Patent No. 5,420,245, U.S. Patent No. 5,523,430, U.S. Patent No. 5,532,359, U.S. Patent No. 5,510,510, U.S. Patent No. 5,589,485, U.S. Patent No. 5,602,098, European Patent Pub]. 0 618 221, European Patent Publ. 0 675 112, European Patent Publ. 0 604 181, European Patent Publ. 0 696 20 593, WO 94/19357, WO 95/08542, WO 95/11917, WO 95/12612, WO 95/12572, WO 95/10514, U.S. Patent No. 5,661,152, WO 95/10515, WO 95/10516, WO 95/24612, WO 95/34535, WO 95/25086, WO 96/05529, WO 96/06138, WO 96/06193, WO 96/16443, WO 96/21701, WO 96/21456, WO 96/22278, WO 96/24611, WO 96/24612, WO 96/05168, WO 96/05169, WO 96/00736, U.S. Patent No. 5,571,792, WO 96/17861, WO 96/33159, WO 25 96/34850, WO 96/34851, WO 96/30017, WO 96/30018, WO 96/30362, WO 96/30363, WO 96/31111, WO 96/31477, WO 96/31478, WO 96/31501, WO 97/00252, WO 97/03047, WO 97/03050, WO 97/04785, WO 97/02920, WO 97/17070, WO 97/23478, WO 97/26246, WO 97/30053, WO 97/44350, WO 98/02436, and U.S. Patent No. 5,532,359. For an example of the role of a prenyl-protein transferase inhibitor on angiogenesis see European J. of Cancer, 30 Vol. 35, No. 9, pp.1394-1401 (1999). Examples of HIV protease inhibitors include amprenavir, abacavir, CGP-73547, CGP-61755, DMP-450, indinavir, nelfinavir, tipranavir, ritonavir, saquinavir, ABT-378, AG 1776, and BMS-232,632. Examples of reverse transcriptase inhibitors include delaviridine, efavirenz, GS-840, HB Y097, lamivudine, nevirapine, AZT, 3TC, ddC, and ddL. - 22 - WO 2004/041164 PCT/US2003/034100 "Angiogenesis inhibitors" refers to compounds that inhibit the formation of new blood vessels, regardless of mechanism. Examples of angiogenesis inhibitors include, but are not limited to, tyrosine kinase inhibitors, such as inhibitors of the tyrosine kinase receptors Flt-1 (VEGFR1) and Flk-1/KDR (VEGFR2), inhibitors of epidermal-derived, fibroblast-derived, or 5 platelet derived growth factors, MMP (matrix metalloprotease) inhibitors, integrin blockers, interferon-a, interleukin-12, pentosan polysulfate, cyclooxygenase inhibitors, including nonsteroidal anti-inflammatories (NSAIDs) like aspirin and ibuprofen as well as selective cyclooxy-genase-2 inhibitors like celecoxib and rofecoxib (PNAS, Vol. 89, p. 7384 (1992); JNCI, Vol. 69, p. 475 (1982); Arch. Opthalmol., Vol. 108, p.

5 7 3 (1990); Anat. Rec., Vol. 238, p. 10 68 (1994); FEBS Letters, Vol. 372, p. 83 (1995); Clin. Orthop. Vol. 313, p. 76 (1995); J. Mol. Endocrinol., Vol. 16, p.

10 7 (1996); Jpn. J. Pharmacol., Vol. 75, p. 105 (1997); Cancer Res., Vol. 57, p. 1625 (1997); Cell, Vol. 93, p. 705 (1998); Intl. J. Mol. Med., Vol. 2, p. 715 (1998); J. Biol. Chem., Vol. 274, p. 9116 (1999)), steroidal anti-inflammatories (such as corticosteroids, mineralocorticoids, dexamethasone, prednisone, prednisolone, methylpred, betamethasone), 15 carboxyamidotriazole, combretastatin A-4, squalamine, 6-O-chloroacetyl-carbonyl)-fumagillol, thalidomide, angiostatin, troponin-1, angiotensin II antagonists (see Fernandez et al., J. Lab. Clin. Med. 105:141-145 (1985)), and antibodies to VEGF (see, Nature Biotechnology, Vol. 17, pp.963-9 6 8 (October 1999); Kim et al., Nature, 362, 841-844 (1993); WO 00/44777; and WO 00/61186). 20 Other therapeutic agents that modulate or inhibit angiogenesis and may also be used in combination with the salts of the instant invention include agents that modulate or inhibit the coagulation and fibrinolysis systems (see review in Clin. Chem. La. Med. 38:679-692 (2000)). Examples of such agents that modulate or inhibit the coagulation and fibrinolysis pathways include, but are not limited to, heparin (see Thromb. Haemost. 80:10-23 (1998)), low 25 molecular weight heparins and carboxypeptidase U inhibitors (also known as inhibitors of active thrombin activatable fibrinolysis inhibitor [TAFIa]) (see Thrombosis Res. 101:329-354 (2001)). TAFIa inhibitors have been described in U.S. Ser. Nos. 60/310,927 (filed August 8, 2001) and 60/349,925(filed January 18, 2002). As described above, the combinations with NSAID's are directed to the use of 30 NSAID's which are potent COX-2 inhibiting agents. For purposes of this specification an NSAID is potent if it possesses an IC50 for the inhibition of COX-2 of 1pM or less as measured by cell or microsomal assays. The invention also encompasses combinations with NSAID's which are selective COX-2 inhibitors. For purposes of this specification NSAID's which are selective inhibitors of 35 COX-2 are defined as those which possess a specificity for inhibiting COX-2 over COX-1 of at -23 - WO 2004/041164 PCT/US2003/034100 least 100 fold as measured by the ratio of IC50 for COX-2 over IC50 for COX-1 evaluated by cell or microsomal assays. Such compounds include, but are not limited to those disclosed in U.S. Patent 5,474,995, issued December 12, 1995, U.S. Patent 5,861,419, issued January 19, 1999, U.S. Patent 6,001,843, issued December 14, 1999, U.S. Patent 6,020,343, issued February 5 1, 2000, U.S. Patent 5,409,944, issued April 25, 1995, U.S. Patent 5,436,265, issued July 25, 1995, U.S. Patent 5,536,752, issued July 16, 1996, U.S. Patent 5,550,142, issued August 27, 1996, U.S. Patent 5,604,260, issued February 18, 1997, U.S. 5,698,584, issued December 16, 1997, U.S. Patent 5,710,140, issued January 20, 1998, WO 94/15932, published July 21, 1994, U.S. Patent 5,344,991, issued June 6, 1994, U.S. Patent 5,134,142, issued July 28, 1992, U.S. 10 Patent 5,380,738, issued January 10, 1995, U.S. Patent 5,393,790, issued February 20, 1995, U.S. Patent 5,466,823, issued November 14, 1995, U.S. Patent 5,633,272, issued May 27, 1997, and U.S. Patent 5,932,598, issued August 3, 1999, all of which are hereby incorporated by reference. Inhibitors of COX-2 that are particularly useful in the instant method of treatment 15 are: 3-phenyl-4-(4-(methylsulfonyl)phenyl)-2-(5H)-furanone; and

SO

2

CH

3 0J 5-chloro-3-(4-methylsulfonyl)phenyl-2-(2-methyl-5-pyridinyl)pyridine;

SO

2

CH

3 CI N 20 N CH 3 or a pharmaceutically acceptable salt thereof. General and specific synthetic procedures for the preparation of the COX-2 inhibitor compounds described above are found in U.S. Patent No. 5,474,995, issued December -24- WO 2004/041164 PCT/US2003/034100 12, 1995, U.S. Patent No. 5,861,419, issued January 19, 1999, and U.S. Patent No. 6,001,843, issued December 14, 1999, all of which are herein incorporated by reference. Compounds that have been described as specific inhibitors of COX-2 and are therefore useful in the present invention include, but are not limited to, the following: O O H2N N

CF

3 5 H 3 C

H

3 C O N

H

2 N-S I NO 0

H

3 C OsN H Et N, O O O or a pharmaceutically acceptable salt thereof. Compounds which are described as specific inhibitors of COX-2 and are 10 therefore useful in the present invention, and methods of synthesis thereof, can be found in the following patents, pending applications and publications, which are herein incorporated by reference: WO 94/15932, published July 21, 1994, U.S. Patent No. 5,344,991, issued June 6, 1994, U.S. Patent No. 5,134,142, issued July 28, 1992, U.S. Patent No. 5,380,738, issued - 25 - WO 2004/041164 PCT/US2003/034100 January 10, 1995, U.S. Patent No. 5,393,790, issued February 20, 1995, U.S. Patent No. 5,466,823, issued November 14, 1995, U.S. Patent No. 5,633,272, issued May 27, 1997, and U.S. Patent No. 5,932,598, issued August 3, 1999. Compounds which are specific inhibitors of COX-2 are therefore useful in the 5 present invention, and methods of synthesis thereof, can be found in the following patents, pending applications and publications, which are herein incorporated by reference: U.S. Patent No. 5,474,995, issued December 12, 1995, U.S. Patent No. 5,861,419, issued January 19, 1999, U.S. Patent No. 6,001,843, issued December 14, 1999, U.S. Patent No. 6,020,343, issued February 1, 2000, U.S. Patent No. 5,409,944, issued April 25, 1995, U.S. Patent No. 5,436,265, 10 issued July 25, 1995, U.S. Patent No. 5,536,752, issued July 16, 1996, U.S. Patent No. 5,550,142, issued August 27, 1996, U.S. Patent No. 5,604,260, issued February 18, 1997, U.S. Patent No. 5,698,584, issued December 16, 1997, and U.S. Patent No. 5,710,140, issued January 20,1998. Other examples of angiogenesis inhibitors include, but are not limited to, 15 endostatin, ukrain, ranpirnase, IM862, 5-methoxy-4-[2-methyl-3-(3-methyl-2-butenyl)oxiranyl] 1-oxaspiro[2,5]oct-6-yl(chloroacetyl)carbamate, acetyldinanaline, 5-amino-l-[[3,5-dichloro-4 (4-chlorobenzoyl)phenyl]methyl]-1H-1,2,3-triazole-4-carboxamide,CM101, squalamine, combretastatin, RP14610, NX31838, sulfated mannopentaose phosphate, 7,7-(carbonyl bis[imino-N-methyl- 4 ,2-pyrrolocarbonyl-imino[N-methyl-4,2-pyrrole]-carbonyliniino]-bis-(1,3 20 naphthalene disulfonate), and 3-[(2,4-dimethylpyrrol-5-yl)methylene]-2-indolinone (SU5416). As used above, "integrin blockers" refers to compounds which selectively antagonize, inhibit or counteract binding of a physiological ligand to the xv03 integrin, to compounds which selectively antagonize, inhibit or counteract binding of a physiological ligand to the axvp5 integrin, to compounds which antagonize, inhibit or counteract binding of a 25 physiological ligand to both the av$3 integrin and the av$5 integrin, and to compounds which antagonize, inhibit or counteract the activity of the particular integrin(s) expressed on capillary endothelial cells. The term also refers to antagonists of the avP6, v08, alp1, 021, a501, at6o1 and 04 integrins. The term also refers to antagonists of any combination of cavp3, av$5, av6, av08, U101, a201, X501, a601 and C64 integrins. 30 Some specific examples of tyrosine kinase inhibitors include N (trifluoromethylphenyl)-5-methylisoxazol-4-carboxaniide, 3-[(2,4-dimethylpyrrol-5 yl)methylidenyl)indolin-2-one, 17-(allylamino)-17-demethoxygeldanamycin, 4-(3-chloro-4 fluorophenylamino)-7-methoxy-6-[3-(4-morpholinyl)propoxyl]quinazoline, N-(3 ethynylphenyl)-6,7-bis(2-methoxyethoxy)-4-quinazolinamine, BIBX1382, 2,3,9,10,11,12 35 hexahydro-10-(hydroxymethyl)-10-hydroxy-9-methyl-9,12-epoxy-lH-diindolo[1,2,3-fg:3',2',1' - 26 - WO 2004/041164 PCT/US2003/034100 kl]pyrrolo [3,4-i] [1,6]benzodiazocin- 1-one, SH268, genistein, STI571, CEP2563, 4-(3 chlorophenylamino)-5,6-dimethyl-7H-pyrrolo[2,3-d]pyrimidinemethane sulfonate, 4-(3-bromo 4-hydroxyphenyl)amino-6,7-dimethoxyquinazoline, 4-(4'-hydroxyphenyl)amino-6,7 dimethoxyquinazoline, SU6668, ST1571A, N-4-chlorophenyl-4-(4-pyridylmethyl)-1 5 phthalazinamine, and EMD121974. The instant compounds are also useful, alone or in combination with platelet fibrinogen receptor (GP JIb/lIIa) antagonists, such as tirofiban, to inhibit metastasis of cancerous cells. Tumor cells can activate platelets largely via thrombin generation. This activation is associated with the release of VEGF. The release of VEGF enhances metastasis by increasing 10 extravasation at points of adhesion to vascular endothelium (Amirkhosravi, Platelets 10, 285 292, 1999). Therefore, the present compounds can serve to inhibit metastasis, alone or in combination with GP fIb/HIa antagonists. Examples of other fibrinogen receptor antagonists include abciximab, eptifibatide, sibrafiban, lamifiban, lotrafiban, cromofiban, and CT50352. Combinations with compounds other than anti-cancer compounds are also 15 encompassed to treat conditions other than cancer. For example, combinations of the instantly claimed compounds with PPAR-y (i.e., PPAR-gamma) agonists are useful in the treatment of diabetic retinopathy. PPAR-y is the nuclear peroxisome proliferator-activated receptor y. The expression of PPAR-y on endothelial cells and its involvement in angiogenesis in corneal and choroidal experimental systems has been reported in the literature (see J. Cardiovasc. 20 Pharmacol. 1998; 31:909-913; J. Biol. Chem. 1999; 274:9116-9121; Invest. Ophthalmol Vis. Sci. 2000; 41:2309-2317). More recently, PPAR-y agonists have been shown to inhibit the angiogenic response to VEGF in vitro; both troglitazone and rosiglitazone maleate inhibit the development of retinal neovascularization in mice. (Arch. Ophthamol. 2001; 119:709-717). Examples of PPAR-y aganoists and PPAR-y//a aganoists include, but are not limited to, 25 thiazolidinediones (such as DRF2725, CS-011, troglitazone, rosiglitazone, and pioglitazone), fenofibrate, gemfibrozil, clofibrate, GW2570, SB219994, AR-H039242, JTT-501, MCC-555, GW2331, GW409544, NN2344, KRP297, NPO1 10, DRF4158, NN622, G1262570, PNU182716, DRF552926, 2-[(5,7-dipropyl-3-trifluoromethyl-1,2-benzisoxazol-6-yl)oxy]-2-methylpropionic acid (disclosed in USSN 09/782,856), and 2(R)-7-(3-(2-chloro-4-(4-fluorophenoxy) 30 phenoxy)propoxy)-2-ethylchromane-2-carboxylic acid (disclosed in USSN 60/235,708 and 60/244,697). Thus, a method of treating or preventing diabetic retinopathy which comprises administering a therapeutically effective amount of a claimed compound in combination with a PPAR-y agonist is also within the scope of the present invention. Another aspect of the invention is illustrated by a composition comprising a 35 therapeutically effective amount of the disclosed kinase inhibitors and a steroidal - 27 - WO 2004/041164 PCT/US2003/034100 antiinflammatory. Steroidal anti-inflammatories include, but are not limited to, corticosteroids, mineralocorticoids, dexamethasone, prednisone, prednisolone, methylpred, and betamethasone. This combination is particularly useful in ophthalmic formulations which may, in some cases, be associated with irritation of the ocular tissues. 5 A particularly useful combination for the treatment of diseases wherein aberrant angiogensis is present involves administering a therapeutically effective amount of the instantly disclosed kinase inhibiting compounds in combination with photodynamic therapy and a photosensitive drug such as verteoporfin (BPD-MA) (Carruth, Clinical Applications of Photodynamic Therapy, Int. J. Clin. Pract. 1998; 52(1):39-42). Such diseases include, but are 10 not limited to, age-related macular degeneration (Bressler, Treatment of Age-Related Macular Degeneration with Photodynamic Therapy Investigation Using Verteoporfin, Invest. Ophthalmol. Vis. Sci. 1998; 39 S242), cancer, especially melanoma and non-melanoma skin cancer, including basal cell and squamous cell carcinomas, (Hassan and Parrish, Photodynamic Therpay in Cancer, Cancer Med. 1997; Dougherty et al., Photodynamic Therapy for the Treatment of Cancer: 15 Current Status and Advances in Photodynamic Therapy of Neoplastic Disease. Kessel (Ed.), CRC Press, 1989; 1-19); Dougherty et al., Photodynamic Therpay, J. Natl. Cancer Inst., 1998, 90(12): 889-905; Jori, Factors Controlling the Selectivity and Efficiency of Tumour Damage in Photodynamic Therapy, Laser Med. Sci. 1990; 5: 115-120; Zhou, Mechanism of Tumour Necrosis Induced by Photodynamic Therapy, J. Photochem. Photobiol. 1989; 3: 299-318), 20 psoriasis (Bissonnette et al., Photodynamic Therapy of Psoriasis and Psoriatic Arthritis with BPD verteporfin. 7 th Biennial Congress, International Photodynamic Association, Nantes, France 1998:73), and rheumatoid arthritis (Hendrich et al., Photodynamic Therapy for Rheumatoid Arthritis. Lasermedizin 11: 73-77 (1995); Hendrich et al. Photodynamic Laser Therapy for Rheumatoid Arthritis: Cell Culture Studies and Animal Experiments, Knee Surg. Sports 25 Traumatol. Arthroscopy 5: 58-63 (1997). Another embodiment of the instant invention is the use of the presently disclosed compounds in combination with gene therapy for the treatment of cancer. For an overview of genetic strategies to treating cancer see Hall et al (An J Hum Genet 61:785-789, 1997) and Kufe et al (Cancer Medicine, 5th Ed, pp 876-889, BC Decker, Hamilton 2000). Gene therapy can be 30 used to deliver any tumor suppresing gene. Examples of such genes include, but are not limited to, p53, which can be delivered via recombinant virus-mediated gene transfer (see U.S. Patent No. 6,069,134, for example), a uPA/uPAR antagonist ("Adenovirus-Mediated Delivery of a uPA/uPAR Antagonist Suppresses Angiogenesis-Dependent Tumor Growth and Dissemination in Mice," Gene Therapy, August 1998;5(8):1105-13), and interferon gamma (JImmunol 35 2000;164:217-222). - 28- WO 2004/041164 PCT/US2003/034100 VEGF receptor tyrosine kinases have been reported to cause a sustained increase in blood pressure in rats when administered more than once, particularly when administered chronically. It is desirable, however, to produce an antiangiogenic effect without causing hypertension. This can be achieved by treating a disease state associated with angiogenesis with 5 a therapeutically effective amount of a combination of an anti-angiogenic agent, such as those presently disclosed, and an anti-hypertensive agent (see WO 01/74360, hereby incorporated by reference). The present invention therefore encompasses a pharmaceutical composition comprising a therapeutically effective amount of a combination of a compound of Formula I and an anti-hypertensive compound. 10 An anti-hypertensive is any agent which lowers blood pressure. There are numerous categories of anti-hypertensive agents including calcium channel blockers, angiotensin converting enzyme inhibitors (ACE inhibitors), angiotensin II receptor antagonists (A-I antagonists), diuretics, beta-adrenergic receptor blockers (p-blockers), vasodilators, alpha adrenergic receptor blockers (a-blockers), selective neutral endopeptidase (NEP) inhibitors and 15 dual ACE-NEP inhibitors. Any anti-hypertensive agent may be used in accordance with this invention and examples from each class are given below. Calcium channel blockers which are within the scope of this invention include, but are not limited to: amlodipine (U.S. Patent No. 4,5 72,909); bepridil (U.S. Patent No. 3,962,23 8 or U.S. Reissue No. 30,577); clentiazem (U.S. Patent No. 4, 567,175); diltiazem (U.S. 20 Patent No. 3,562,257); fendiline (U.S. Patent No. 3,262,977); gallopamil (U.S. Patent No. 3,261,859); mibefradil (U.S. Patent No. 4,808,605); prenylamine (U.S. Patent No. 3,152,173); semotiadil (U.S. Patent No. 4,786,63 5); terodiline (U.S. Patent No. 3,3 71,014); verapamil (U.S. Patent No. 3,261,859); aranidipine (U.S. Patent No. 4, 446,325); bamidipine (U.S. Patent No. 4,220,649); benidipine (European Patent Application Publication No. 106,275); cilnidipine (U.S. 25 Patent No. 4,672,068); efonidipine (U.S. Patent No. 4,885,284);elgodipine (U.S. Patent No. 4,952,592); felodipine (U.S. Patent No. 4, 264,611); isradipine (U.S. Patent No. 4,466,972); lacidipine (U.S. Patent No. 4,801,599); lercanidipine (U.S. Patent No. 4,705,797); manidipine (U.S. Patent No. 4,892,875); nicardipine (U.S. Patent No. 3,985,758); nifedipine (U.S. Patent No. 3,485,847); nilvadipine (U.S. Patent No. 4,338,322); nimodipine (U.S. Patent No. 30 3,799,934); nisoldipine (U.S. Patent No. 4,154,83 9); nitrendipine (U.S. Patent No. 3,799,934); cinnarizine (U.S. Patent No. 2,882,271); flunarizine (U.S. Patent No. 3,773,93 9); lidoflazine (U.S. Patent No. 3,267,104); lomerizine (U.S. Patent No. 4,663,325); bencyclane (Hungarian Patent No. 151,865); etafenone (German Patent No. 1,265,758); and perhexiline (British Patent No. 1,025,578). The disclosures of all such patents and patent applications are incorporated 35 herein by reference. - 29 - WO 2004/041164 PCT/US2003/034100 - Angiotensin Converting Enzyme Inhibitors (ACE-Inhibitors) which are within the scope of this invention include, but are not limited to: alacepril (U.S. Patent No. 4,248,883); benazepril (U.S. Patent No. 4,410,520); captopril (U.S. Patents Nos. 4, 046,889 and 4,105,776); ceronapril (U.S. Patent No. 4,452,790); delapril. (U.S. Patent No. 4,385,05 1); enalapril (U.S. 5 Patent No. 4,374,829); fosinopril (U.S. Patent No. 4,337,201); imidapril (U.S. Patent No. 4,508,727); lisinopril (U.S. Patent No. 4,555,502); moveltipril (Belgium Patent No. 893,553); perindopril (U.S. Patent No. 4,508,729); quinapril (U.S. Patent No. 4,344,949); ramipril (U.S. Patent No. 4,587,258); spirapril (U.S. Patent No. 4,470,972); temocapril (U.S. Patent No. 4,699,905); and trandolapril (U.S. Patent No. 4,933,361). The disclosures of all such patents are 10 incorporated herein by reference. Angiotensin-II receptor antagonists (A-Il antagonists) which are within the scope of this invention include, but are not limited to: candesartan (U.S. Patent No. 5,196,444); eprosartan (U.S. Patent No. 5,185,351); irbesartan (U.S. Patent No. 5,270,317); losartan (U.S. Patent No. 5,138,069); and valsartan (U.S. PatentNo. 5,399,578. The disclosures of all such U.S. 15 patents are incorporated herein by reference. p-Blockers which are within the scope of this invention include, but are not limited to: acebutolol (U.S. Patent No. 3,857,952); alprenolol (Netherlands Patent Application No. 6,605,692); amosulalol (U.S. Patent No. 4,217,305); arotinolol (U.S. Patent No. 3,932,400); atenolol (U.S. Patents Nos. 3,663,607 and 3,836,671); befunolol (U.S. Patent No. 3,853,923); 20 . betaxolol (U.S. Patent No. 4,252,984); bevantolol (U.S. Patent No. 3,857,891); bisoprolol (U.S. Patent No. 4,258,062); bopindolol (U.S. Patent No. 4,340,541); bucumolol (U.S. Patent No. 3,663,570); bufetolol (U.S. Patent No. 3,723,476); bufuralol (U.S. Patent No. 3,929,836); bunitrolol (U.S. Patent No. 3,541,130); bupranolol (U S. Patent No. 3,309,406); butidrine hydrochloride (French Patent No. 1,390,056); butofilolol (U.S. Patent No. 4,302,601); carazolol 25 (German Patent No. 2,240,599); carteolol (U.S. Patent No. 3,910,924); carvedilol (U.S. PatentNo. 4,503,067); celiprolol (U.S. Patent No. 4,034,009); cetamolol (U.S. Patent No. 4,059,622); cloranolol (German Patent No. 2, 213,044); dilevalol (Clifton et al., Journal of Medicinal Chemistry, 1982, 25, 670); epanolol (U.S. Patent No. 4,167,58 1); indenolol (U.S. Patent No. 4,045,482); labetalol (U.S. Patent No. 4,012,444); levobunolol (U.S. Patent No. 30 4,463,176); mepindolol (Seeman et al, Helv. Chim. Acta, 1971, 54, 2411); metipranolol (Czechoslovakian Patent Application No. 128,471); metoprolol (U.S. Patent No. 3,873,600); moprolol (U.S. Patent No. 3,501,769); nadolol (U.S. Patent No. 3,935,267); nadoxolol (U.S. Patent No. 3,819,702); nebivalol (U.S. Patent No. 4,654,3 62); nipradilol (U.S Patent No. 4,394,382); oxprenolol (British Patent No. 1, 077,603); penbutolol (U.S. Patent No. 3,551,493); 35 pindolol (Swiss Patents Nos. 469,002 and 472,404); practolol (U.S. Patent No. 3,408,387); - 30 - WO 2004/041164 PCT/US2003/034100 pronethalol (British Patent No. 909,357); propranolol (U.S. Patents Nos. 3,337,628 and 3,520,919); sotalol (Uloth et al., Journal of Medicinal Chemistry, 1966, 9, 88); sulfinalol (German Patent No. 2,728,641); talinolol (U.S. Patents Nos. 3,935,259 and 4,038,313); tertatolol (U.S. Patent No. 3,960,891); tilisolol (U.S. Patent No. 4,129,565); timolol (U.S. Patent No. 5 3,655,663); toliprolol (U.S. Patent No. 3,432,545); and xibenolol (U.S. Patent No. 4, 018,824). The disclosures of all such patents, patent applications and references are incorporated herein by reference. a-Blockers which are within the scope of this invention include, but are not limited to: amosulalol (U.S. Patent No. 4,217,305); arotinolol; dapiprazole (U.S. Patent No. 10 4,252,721); doxazosin (U.S. Patent No. 4,188,390); fenspiride (U.S. Patent No. 3,399,192); indoramin (U.S. Patent No. 3,527,761); labetolol; naftopidil (U.S. Patent No. 3,997,666); nicergoline (U.S. Patent No. 3,228,943); prazosin (U.S. Patent No. 3,511,836); tainsulosin (U.S. Patent No. 4,703,063); tolazoline (U.S. Patent No. 2,161,93 8); trimazosin (U.S. Patent No. 3,669,968); and yohimbine. The disclosures of all such U.S. patents are incorporated herein by 15 reference. The term "vasodilator" as used herein is meant to include cerebral vasodilators, coronary vasodilators and peripheral vasodilators. Cerebral vasodilators within the scope of this invention include, but are not limited to: bencyclane; cinnarizine; citicoline; cyclandelate (U.S. Patent No. 3,663,597); ciclonicate (German Patent No. 1,910,481); diisopropylamine 20 dichloroacetate (British Patent No. 862,248); ebumamonine (Hermann et al., Journal of the American Chemical Society, 1979, 101, 1540); fasudil (U.S. Patent No. 4,678,783); fenoxedil (U.S. Patent No. 3,818,021); flunarizine (U.S. Patent No. 3,773,93 9); ibudilast (U.S. Patent No. 3,850,941); ifenprodil (U.S. Patent No. 3,509,164); lomerizine (U.S. Patent No. 4,663,325); nafronyl (U.S. Patent No. 3,334,096); nicametate (Blicke et al., Journal of the American 25 Chemical Society, 1942, 64, 1722); nicergoline; nimodipine (U.S. Patent No. 3,799,934); papaverine (Goldberg, Chem. Prod. Chem. News, 1954, 17, 37 1; pentifylline (German Patent No. 860,217); tinofedrine (U.S. Patent No. 3,767,675); vincamine (U.S. Patent No. 3,770,724); vinpocetine (U.S. Patent No. 4,035,750); and viquidil (U.S. Patent No. 2,500,444). The disclosures of all such patents and references are incorporated herein by reference. Coronary 30 vasodilators within the scope of this invention include, but are not limited to: amotriphene (U.S. Patent No. 3,010,965); bendazol (Feitelson, et al., J. Chem. Soc. 1958, 2426); benfurodil hemisuccinate (U.S. Patent No. 3,355,463); benziodarone (U.S. Patent No. 3,012,042); chloracizine (British Patent No. 740,932) chromonar (U.S. Patent No. 3,282,93 8); clobenfural (British Patent No. 1,160,925); clonitrate; cloricromen (U.S. Patent No. 4,452,811); dilazep 35 (U.S. Patent No. 3,532,685); dipyridamole (British Patent No. 807,826); droprenilamine -31- WO 2004/041164 PCT/US2003/034100 (German Patent No. 2,521,113); efloxate (British Patents Nos. 803,372 and 824,547); erythrityl tetranitrate; etafenone (German Patent No. 1,265,75 8); fendiline (U.S. Patent No. 3,262,977); floredil (German Patent No. 2,020,464); ganglefene (U.S.S.R. Patent No. 115,905); hexestrol bis(P-diethylaminoethyl) ether (Lowe et al. , J. Chem. Soc. 1951, 3286); hexobendine (U.S. 5 Patent No. 3,267,103); itramin tosylate (Swedish Patent No. 168,3 08); khellin (Baxter et al., Journal of the Chemical Society, 1949, S 30); lidoflazine (U.S. Patent No. 3,267,104); mannitol hexanitrate; medibazine (U.S. Patent No. 3,119,826); nitroglycerin; pentaerythritol tetranitrate; pentrinitrol (German Patent No. 638,422-3); perhexiline; pimefylline (U.S. Patent No. 3,350,400); prenylamine (U.S. Patent No. 3,152,173); propatyl nitrate (French Patent No. 10 1,103,113); trapidil (East German Patent No. 5 5,956); tricromyl (U.S. Patent No. 2,769,015); trimetazidine (U.S. Patent No. 3,262,852); trolnitrate phosphate; visnadine (U.S. Patents Nos. 2,816,118 and 2,980,699. The disclosures of all such patents and references are incorporated herein by reference. Peripheral vasodilators within the scope of this invention include, but are not limited to: aluminium nicotinate (U.S. Patent No. 2,970,082); bamethan (Corrigan et al., 15 Journal of the American Chemical Society, 1945, 67, 1894); bencyclane; betahistine (Walter et al, Journal of the American Chemical Society, 1941, 63); bradykinin; brovincamine (U.S. Patent No. 4,146,643); bufeniode (U.S. Patent No. 3,542,870); buflomedil (U.S. Patent No. 3,895,030); butalamine (U.S. Patent No. 3,338,899); cetiedil (French Patent No. 1,460,571); ciclonicate (German Patent No. 1,910,481); cinepazide (Belgium Patent No. 730,345); cinnarizine; 20 cyclandelate; diisopropylamine dichloroacetate; eledoisin (British Patent No. 984,810); fenoxedil; flunarizine; hepronicate (U.S. Patent No. 3,384,642); ifenprodil; iloprost (U.S. Patent No. 4,692,464); inositol niacinate (Badgett et al., Journal of the American Chemical Society, 1947, 69, 2907); isoxsuprine (U.S. Patent No. 3.056,836); kallidin (Nicolaides et al., Biochem. Biophys. Res. Commun., 1961, 6, 210); kallikrein (German Patent No. 1,102,973); moxisylyte 25 (German Patent No. 905,738); nafronyl; nicametate; nicergoline; nicofaranose (Swiss Patent No. 366,523); nylidrin (U.S. Patent Nos. 2,661,372 and 2,661,373); pentifylline; pentoxifylline (U.S. Patent No. 3,422,107); piribedil (U.S. Patent No. 3,299,067); prostaglandin El (Merck Index, Twelfth Edition, Budaveri, Ed, New Jersey 1996, page 1353); suloctidil (German Patent No. 2,334,404); tolazoline (U.S. Patent No. 2,161,938); and xanthinol niacinate (German Patent No. 30 1,102,750). The disclosures of all such patents and references are incorporated herein by reference. The term "diuretic" as used herein includes but is not limited to diuretic benzothiadiazine derivatives, diuretic organomercurials, diuretic purines, diuretic steroids, diuretic sulfonamide derivatives, diuretic uracils and other diuretics such as amanozine (Austrian 35 Patent No. 168,063); amiloride (Belgium Patent No. 639,386); arbutin (Tschitschibabin et al., -32- WO 2004/041164 PCT/US2003/034100 Annalen, 1930, 479, 303); chlorazanil(Austrian Patent No. 168,063); ethacrynic acid (U.S. Patent No. 3,255,241); etozolin (U.S. Patent No. 3,072,653); hydracarbazine (British Patent No. 856,409); isosorbide (U.S. Patent No. 3,160,641); mannitol; metochalcone (Freudenberg et al., Ber., 1957, 90, 957); muzolimine (U.S. Patent No. 4,018,890); perhexiline; ticrynafen (U.S. 5 Patent No. 3,758,506); triamterene (U.S. Patent No. 3,081,230); and urea. The disclosures of all such patents and references are incorporated herein by reference. Diuretic benzothiadiazine derivatives within the scope of this invention include, but are not limited to: althiazide (British Patent No. 902,658); bendroflumethiazide (U.S. Patent No. 3,392,168); benzthiazide (U.S. Patent No. 3,440,244); benzyl hydrochlorothiazide (U.S. Patent No. 3,108,097); buthiazide 10 (British Patent Nos. 861,367 and 885,078); chlorothiazide (U.S. Patent Nos. 2,809,194 and 2,937,169);chlorthalidone (U.S. Patent No. 3,055,904); cyclopenthiazide (Belgium Patent No. 587,225); cyclothiazide (Whitehead et al Journal of Organic Chemistry, 1961, 26, 2814); epithiazide (U.S. Patent No. 3,009,911); ethiazide (British Patent No. 861,367); fenquizone (U.S. Patent No. 3,870,720); indapamide (U.S. Patent No. 3,565,911); hydrochlorothiazide (U.S. 15 Patent No. 3,164,588); hydroflumethiazide (U.S. Patent No. 3,254,076); methyclothiazide (Close et al., Journal of the American Chemical Society, 1960, 82, 1132); meticrane (French Patent Nos. M2790 and 1,365,504); metolazone (U.S. Patent No. 3,360,518); paraflutizide (Belgium Patent No. 15 620,829); polythiazide (U.S. Patent No. 3,009,911); quinethazone (U.S. Patent No. 2,976,289); teclothiazide (Close et al., Journal of the American Chemical Society, 1960, 82, 20 1132); and trichlormethiazide (deStevens et al., Experientia, 1960, 16, 113). The disclosures of all such patents and references are incorporated herein by reference. Diuretic sulfonamide derivatives within the scope of this invention include, but are not limited to: acetazolamide (U.S. Patent No. 2,554,816); ambuside (U.S. Patent No. 3,188,329); azosemide (U.S. Patent No. 3,665,002); bumetanide (U.S. Patent No, 3,806,534); butazolamide (British Patent No. 769,757); 25 chloraminophenamide (U.S. Patent Nos. 2,909,194; 2,965,655; and 2,965,656); clofenamide (Olivier, Rec. Trav. Chim., 1918, 37, 307); clopamide (U. S. Patent No. 3,459,756); clorexolone (U.S. Patent No. 3,183,243); disulfamide (British Patent No. 851,287); ethozolamide (British Patent No. 795,174); furosemide (U.S. Patent No. 3,058,882); mefruside (U.S. Patent No.3,356,692); methazolamide (U.S. Patent No. 2,783,24 1); piretanide (U.S. Patent No. 4, 30 010,273); torsemide (U.S. Patent No. 4,018,929); tripamide (Japanese Patent No. 305,585); and xipamide (U.S. Patent No. 3,567,777). The disclosures of all such patents and references are incorporated herein by reference. Selective neutral endopeptidase inhibitors are taught by Delaney et al. in U.S. Patent Nos. 4,722,810 and 5,223,516 and the use of selective neutral endopeptidase inhibitors 35 alone or in combination with angiotensin converting enzyme inhibitors to treat hypertension are - 33 - WO 2004/041164 PCT/US2003/034100 disclosed by Delaney et al. U.K. Patent Application No. 2,207,351 and by Haslanger et al. in U.S. Patent No. 4,749,688. Compounds possessing both neutral endopeptidase and angiotensin converting enzyme inhibition activity are disclosed by Flynn et al. in U.S. Patent No.5,366,973, European Patent Application No. 481,522 and PCT Patent Applications Nos. WO 93/16103, and 5 WO 94/10193, Warshawsky et al. European Patent Applications Nos. 534,363, 534,396 and 534,492, Fournie- Zaluski European Patent Application No. 524,553, Karanewsky et al. European Patent Application No. 599,444, Karanewsky European Patent Application No. 595, 610, Robl et al., European Patent Application No. 629,627, Robl, U.S. Patent No. 5,362,727 and European Patent Application No. 657,453. The disclosures of all such patents and publications 10 are incorporated herein by reference. Further, the anti-hypertensive agents which may be used in accordance with this invention and the pharmaceutically acceptable salts thereof may occur as prodrugs, hydrates or solvates. Said hydrates and solvates are also within the scope of the present invention. Preferred anti-hypertensive agents of the invention include, calcium channel blockers, A-I antagonists, 15 ACE inhibitors and p-blockers. More preferred anti-hypertensive agents of the invention include ACE inhibitors, particularly lisinopril, enalapril and captopril, and A-Il antagonists, particularly losartan. The anti-hypertensives described herein are generally commercially available, or they may be made by standard techniques including those described in the references cited above. The instant compounds are also useful, alone, or in combination with ovulation 20 stimulators such.as, but not limited to; bromocriptine (e.g., PARLODEL), luprolide (e.g., LUPRON), clomifene (e.g., CLOMID, SEROPHENE) and pharmaceutically acceptable salts thereof, follicle stimulating hormone (e.g., FERTINEX/ METRODIN, FOLLISTIM, GONAL F), menopausal gonadotropin or mentropins (e.g., REPRONEX), chorionic gonadotropin (e.g., PROFASI, PREGNYL), luteinizing hormone releasing hormone (e.g., GONADORELIN), 25 luteinizing hormone and combinations thereof to treat or prevent ovarian hyper-stimulation syndrome (OHSS). OHSS is a side effect that occurs during infertility treatment with ovulation inducing drugs. OHSS has also been reported to occur as a result of increased endogenous seceretion of gonadotropins (Obstet. Gynecol. 21:28, 1963; J. Obstet. Gynaecol. Br. Commonw. 74:451, 1967). Symptoms of OHSS range from mild to critical and are associated with ovarian 30 enlargement and increased vascular permeability. Women with the most severe symptoms demonstrate increased VEGF levels in follicular fluids that are reversed via the addition of a VEGF antibody indicating that VEGF is responsible for vascular permeabiliy contributing to the pathogenesis of OHSS. Levin, E.R. et al., J. Clin. Invest. 102, 1978-1985 (1998). Therefore, a "method of treating or preventing ovarian hyper-stimulation syndrome, which comprises -34- WO 2004/041164 PCT/US2003/034100 administering a therapeutically effective amount of a claimed compound, alone, or in combination with an ovulation stimulator is within the scope of the present invention. If formulated as a fixed dose, such combination products employ the compounds of this invention within the dosage range described below and the other pharmaceutically active 5 agent(s) within its approved dosage range. Compounds of the instant invention may alternatively be used sequentially with known pharmaceutically acceptable agent(s) when a combination formulation is inappropriate. The term "administration" and variants thereof (e.g., "administering" a compound) in reference to a compound of the invention means introducing the compound or a 10 prodrug of the compound into the system of the animal in need of treatment. When a compound of the invention or prodrug thereof is provided in combination with one or more other active agents (e.g., a cytotoxic agent, etc.), "administration" and its variants are each understood to include concurrent and sequential introduction of the compound or prodrug thereof and other agents. 15 As used herein, the term "composition" is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combination of the specified ingredients in the specified amounts. The term "therapeutically effective amount" as used herein means that amount of 20 active compound or pharmaceutical agent that elicits the biological or medicinal response in a tissue, system, animal or human that is being sought by a researcher, veterinarian, medical doctor or other clinician. The term "treating cancer" or "treatment of cancer" refers to administration to a mammal afflicted with a cancerous condition and refers to an effect that alleviates the cancerous 25 condition by killing the cancerous cells, but also to an effect that results in the inhibition of growth and/or metastasis of the cancer. The present invention also encompasses a pharmaceutical composition useful in the treatment of cancer, comprising the administration of a therapeutically effective amount of the compounds of this invention, with or without pharmaceutically acceptable carriers or 30 diluents. Suitable compositions of this invention include aqueous solutions comprising compounds of this invention and pharmaceutically acceptable carriers, e.g., saline, at a pH level, e.g., 7.4. The solutions may be introduced into a patient's bloodstream by local bolus injection. When a compound according to this invention is administered into a human subject, the daily dosage will normally be determined by the prescribing physician with the -35- WO 2004/041164 PCT/US2003/034100 dosage generally varying according to the age, weight, and response of the individual patient, as well as the severity of the patient's symptoms. In one exemplary application, a suitable amount of compound is administered to a mammal undergoing treatment for cancer. Administration occurs in an amount between about 5 0.1 mg/kg of body weight to about 60 mg/kg of body weight per day, preferably of between 0.5 mg/kg of body weight to about 40 mg/kg of body weight per day. The scope of the invetion therefore encompasses the use of the instantly claimed compounds in combination with a second compound selected from: 1) an estrogen receptor modulator, 10 2) an androgen receptor modulator, 3) retinoid receptor modulator, 4) a cytotoxic agent, 5) an antiproliferative agent, 6) a prenyl-protein transferase inhibitor, 15 7) an HIG-CoA reductase inhibitor, 8) an IV protease inhibitor, 9) a reverse transcriptase inhibitor, and 10) another angiogenesis inhibitor. Preferred angiogenesis inhibitors to be used as the second compound are a 20 tyrosine kinase inhibitor, an inhibitor of epidermal-derived growth factor, an inhibitor of fibroblast-derived growth factor, an inhibitor of platelet derived growth factor, an MMP (matrix metalloprotease) inhibitor, an integrin blocker, interferon-a, interleukin-12, pentosan polysulfate, a cyclooxygenase inhibitor, carboxyamidotriazole, combretastatin A-4, squalamine, 6-0 (chloroacetyl-carbonyl)-fumagillol, thalidomide, angiostatin, troponin-1, or an antibody to 25 VEGF. Preferred estrogen receptor modulators are tamoxifen and raloxifene. Also included in the scope of the claims is a method of treating cancer which comprises administering a therapeutically effective amount of a claimed compound in combination with radiation therapy and/or in combination with a compound selected from: 1) an estrogen receptor modulator, 30 2) an androgen receptor modulator, 3) retinoid receptor modulator, 4) a cytotoxic agent, 5) an antiproliferative agent, 6) a prenyl-protein transferase inhibitor, 35 7) an BMG-CoA reductase inhibitor, - 36 - WO 2004/041164 PCT/US2003/034100 8) an IiV protease inhibitor, 9) a reverse transcriptase inhibitor, and 10) another angiogenesis inhibitor. And yet another embodiment of the invention is a method of treating cancer 5 which comprises administering a therapeutically effective amount of a compound of Formual I in combination with paclitaxel or trastuzumab. The invention further encompasses a method of treating or preventing cancer, which comprises administering a therapeutically effective amount of a claimed compound in combination with a COX-2 inhibitor. 10 These and other aspects of the invention will be apparent from the teachings contained herein. Definitions As used herein, "dual inhibitor" refers to a compound with enzyme IC50 < 1 [LM 15 against both a tyrosine kinase (KDR) and a cyclin-dependent kinase (Cdk4). Dual inhibitor also refers to a compound with cell-based inhibition of both Rb phosphorylation and KDR autophosphorylation of IC50 < 10 pM. The ratio of dual inhibitory activity is preferably within about 50-fold of each other. Most preferably the ratio is within about 10-fold of each other. The compounds of the present invention may have asymmetric centers, chiral 20 axes, and chiral planes (as described in: E.L. Eliel and S.H. Wilen, Stereochemistry of Carbon Compounds, John Wiley & Sons, New York, 1994, pp. 1119-1190), and occur as racemates, racemic mixtures, and as individual diastereomers, with all possible isomers and mixtures thereof, including optical isomers, being included in the present invention. In addition, the compounds disclosed herein may exist as tautomers and both tautomeric forms are intended to be 25 encompassed by the scope of the invention, even though only one tautomeric structure is depicted. For example, any claim to compound A below is understood to include tautomeric structure B, and vice versa, as well as mixtures thereof. OH 0 N R 2 N A B When any variable occurs more than one time in any constituent, its definition on 30 each occurrence is independent at every other occurrence. Also, combinations of substituents and variables are permissible only if such combinations result in stable compounds. Lines drawn - 37 - WO 2004/041164 PCT/US2003/034100 into the ring systems from substituents indicate that the indicated bond may be attached to any of the substitutable ring carbon atoms. If the ring system is polycyclic, it is intended that the bond be attached to any of the suitable carbon atoms on the proximal ring only. It is understood that substituents and substitution patterns on the compounds of 5 the instant invention can be selected by one of ordinary skill in the art to provide compounds that are chemically stable and that can be readily synthesized by techniques known in the art, as well as those methods set forth below, from readily available starting materials. If a substituent is itself substituted with more than one group, it is understood that these multiple groups may be on the same carbon or on different carbons, so long as a stable structure results. The phrase 10 "optionally substituted with one or more substituents" should be taken to be equivalent to the phrase "optionally substituted with at least one substituent" and in such cases the preferred embodiment will have from zero to three substituents. As used herein, "alkyl" is intended to include both branched, straight-chain, and cyclic saturated aliphatic hydrocarbon groups having the specified number of carbon atoms. For 15 example, C1-C10, as in "Ci-Clo alkyl" is defined to include groups having 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 carbons in a linear or branched, arrangement and may be cyclic or acyclic. For example, "CI-CiO alkyl" specifically includes methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, and so on, as well as cyclo-alkyls such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, tetrahydronaphthalene, methylenecylohexyl, and so on. In some instances, 20 definitions may appear for the same variable reciting both alkyl and cycloalkyl when a different number of carbons is intended for the respective substituents. The use of both terms in one definition should not be interpreted to mean in another definition that "alkyl" does not encompass "cycloalkyl" when only "alkyl" is used. "Alkoxy" represents an alkyl group of indicated number of carbon atoms as 25 defined above attached through an oxygen bridge. If no number of carbon atoms is specified, the term "alkenyl" refers to a non aromatic hydrocarbon radical, which may be branched or unbranched and cyclic or acyclic, containing from 2 to 10 carbon atoms and at least one carbon to carbon double bond. Preferably one carbon to carbon double bond is present, and up to four non-aromatic carbon-carbon double 30 bonds may be present. Thus, "C2-C6 alkenyl" means an alkenyl radical having from 2 to 6 carbon atoms. Alkenyl groups include ethenyl, propenyl, butenyl, 2-methylbutenyl, cyclohexenyl, methylenylcyclohexenyl, and so on. The term "alkynyl" refers to a hydrocarbon radical, which may be branched or unbranched and cyclic or acyclic, containing from 2 to 10 carbon atoms and at least one carbon 35 to carbon triple bond. Up to three carbon-carbon triple bonds may be present. Thus, "C2-C6 - 38 - WO 2004/041164 PCT/US2003/034100 alkynyl" means an alkynyl radical having from 2 to 6 carbon atoms. Alkynyl groups include ethynyl, propynyl, butynyl, 3-methylbutynyl and so on. In certain instances, substituents may be defined with a range of carbons that includes zero, such as (CO-C6)alkylene-aryl. If aryl is taken to be phenyl, this definition would 5 include phenyl itself as well as -CH2Ph, -CH2CH2Ph, -CH(CH3)CH2CH(CH3)Ph, and so on. As used herein, "aryl" is intended to mean phenyl and substituted phenyl, including moieties with a fused benzo group. Examples of such aryl elements include phenyl, naphthyl, tetrahydro-naphthyl, indanyl, biphenyl, phenanthryl, anthryl or acenaphthyl. In cases where the aryl substituent is bicyclic, it is understood that attachment is via the phenyl ring. 10 Unless otherwise indicated, "aryl" includes phenyls substituted with one or more substituents. The term heteroaryl, as used herein, represents a stable monocyclic or bicyclic ring of up to 7 atoms in each ring, wherein at least one ring is aromatic and contains from 1 to 4 heteroatoms selected from the group consisting of 0, N and S. Heteroaryl groups within the scope of this definition include but are not limited to: acridinyl, carbazoly1, cinnolinyl, 15 quinoxalinyl, pyrrazolyl, indolyl, benzotriazolyl, furanyl, thienyl, benzothienyl, benzofuranyl, quinolinyl, isoquinolinyl, oxazolyl, isoxazolyl, indolyl, pyrazinyl, pyridazinyl, pyridinyl, pyrimidinyl, pyrrolyl, tetrahydroquinoline. As with the definition of heterocycle below, "heteroaryl" is also understood to include the N-oxide derivative of any nitrogen-containing heteroaryl. In cases where the heteroaryl substituent is bicyclic and one ring is non-aromatic or 20 contains no heteroatoms, it is understood that attachment is via the aromatic ring or via the heteroatom containing ring, respectively. As appreciated by those of skill in the art, "halo" or "halogen" as used herein is intended to include chloro, fluoro, bromo and iodo. The term "heterocycle" or "heterocyclyl" as used herein is intended to mean a 5 25 to 10-membered aromatic or nonaromatic heterocycle containing from 1 to 4 heteroatoms selected from the group consisting of 0, N and S, and includes bicyclic groups. "Heterocyclyl" therefore includes the above mentioned heteroaryls, as well as dihydro and tetrathydro analogs thereof. Further examples of "heterocyclyl" include, but are not limited to the following: benzoimidazolyl, benzofuranyl, benzofurazanyl, benzopyrazolyl, benzotriazolyl, 30 benzothiophenyl, benzoxazolyl, carbazolyl, carbolinyl, cinnolinyl, furanyl, imidazolyl, indolinyl, indolyl, indolazinyl, indazolyl, isobenzofuranyl, isoindolyl, isoquinolyl, isothiazolyl, isoxazolyl, naphthpyridinyl, oxadiazolyl, oxazolyl, oxazoline, isoxazoline, oxetanyl, pyranyl, pyrazinyl, pyrazolyl, pyridazinyl, pyridopyridinyl, pyridazinyl, pyridyl, pyrimidyl, pyrrolyl, quinazolinyl, quinolyl, quinoxalinyl, tetrahydropyranyl, tetrazolyl, tetrazolopyridyl, thiadiazolyl, 35 thiazolyl, thienyl, triazolyl, azetidinyl, aziridynyl, 1,4-dioxanyl, hexahydroazepinyl, piperazinyl, - 39 - WO 2004/041164 PCT/US2003/034100 piperidinyl, pyrrolidinyl, morpholinyl, thiomorpholinyl, dihydrobenzoimidazolyl, dihydrobenzofuranyl, dihydrobenzothiophenyl, dihydrobenzoxazolyl, dihydrofuranyl, dihydroimidazolyl, dihydroindolyl, dihydroisooxazolyl, dihydroisothiazolyl, dihydrooxadiazolyl, dihydrooxazolyl, dihydropyrazinyl, dihydropyrazolyl, dihydropyridinyl, dihydropyrimidinyl, 5 dihydropyrrolyl, dihydroquinolinyl, dihydrotetrazolyl, dihydrothiadiazolyl, dihydrothiazolyl, dihydrothienyl, dihydrotriazolyl, dihydroazetidinyl, methylenedioxybenzoyl, tetrahydrofuranyl, and tetrahydrothienyl, and N-oxides thereof. Attachment of a heterocyclyl substituent can occur via a carbon atom or via a heteroatom.

R

5 is heterocyclyl. It is understood that attachment of any substituents may occur 10 via a carbon atom or a heteroatom. The alkyl, alkenyl, alkynyl, cycloalkyl, aryl, heteroaryl and heterocyclyl substituents may be substituted or unsubstituted, unless specifically defined otherwise. For example, a (C1 -C6)alkyl may be substituted with one or more substituents selected from OH, oxo, halogen, alkoxy, dialkylamino, or heterocyclyl, such as morpholinyl, piperidinyl, and so on. 15 In this case, if one substituent is oxo and the other is OH, the following are included in the definition: -(C=O)CH2CH(OH)CH3, -(C=O)OH, -CH2(OH)CH2CH(O), and so on. The pharmaceutically acceptable salts of the compounds of this invention include the conventional non-toxic salts of the compounds of this invention as formed inorganic or organic acids. For example, conventional non-toxic salts include those derived from inorganic 20 acids such as hydrochloric, hydrobromic, sulfuric, sulfamic, phosphoric, nitric and the like, as well as salts prepared from organic acids such as acetic, propionic, succinic, glycolic, stearic, lactic, malic, tartaric, citric, ascorbic, pamoic, maleic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, sulfanilic, 2-acetoxy-benzoic, fumaric, toluenesulfonic, methanesulfonic, ethane disulfonic, oxalic, isethionic, trifluoroacetic and the like. The preparation of the 25 pharmaceutically acceptable salts described above and other typical pharmaceutically acceptable salts is more fully described by Berg et al., "Pharmaceutical Salts," J. Pharm. Sci., 1977:66:1-19, hereby incorporated by reference. The pharmaceutically acceptable salts of the compounds of this invention can be synthesized from the compounds of this invention which contain a basic or acidic moiety by conventional chemical methods. Generally, the salts of the basic compounds 30 are prepared either by ion exchange chromatography or by reacting the free base with stoichiometric amounts or with an excess of the desired salt-forming inorganic or organic acid in a suitable solvent or various combinations of solvents. Similarly, the salts of the acidic compounds are formed by reactions with the appropriate inorganic or organic base. -40- WO 2004/041164 PCT/US2003/034100 Preferably R 1 and R 2 are independently H, CN, halogen, OH, phenyl, wherein said phenyl is optionally substituted with one or more substituents selected from R 6 , (C=O)rOs(C1-C1O)alkyl and (C=O)rOs(Ci-C1O)alkyl-NRaRb.

R

4 is H, CN, halogen, (Ci-C6)alkyl or (Ci-C6)perfluoroalkyl. 5 Most preferably RI and R 3 are H, R 2 is CN or phenyl and R 4 is H or (Ci C6)alkyl. Preferably R 5 is: RE R6 R

R

6 N N N6 N N R N 6 R s--'\ R R 0 9 , or N 10 R5 may be substituted with one or more substituents selected from

R

6 and R 6 can be bound to any atom on the ring system SCHEMES 15 The compounds of this invention may be prepared by employing reactions as shown in the following schemes, in addition to other standard manipulations that are known in the literature or exemplified in the experimental procedures. These schemes, therefore, are not limited by the compounds listed or by any particular substituents employed for illustrative purposes. Substituent numbering as shown in the schemes does not necessarily correlate to that 20 used in the claims. The compounds of the instant invention may be prepared from the general reaction schemes for pyrimidine analogs. -41- WO 2004/041164 PCT/US2003/034100 SCHEME A OH R N

R

5 -OH N

R

4 N NH Ph 3 P/ DEAD 4 N A-1 2 THF R A-2 NH2 N R2 R5sO Cl S A-3 N N NaH/THF R4 N N S H A-4 SCHEME B OH Cl N heat N R 5 -OH 11 NaH/ toluene R N NH 2 R4N NH 2 heat A-1 B-1 R5 0 N \ -R 2 R 5_O N> Ci A-3 N>

R

4 BN NH 2 Xn1phosi Rc4t N N S R Dioxane A-4 H 5 heat -42- WO 2004/041164 PCT/US2003/034100 SCHEME C OH CI N R 2 N heat N CI S A-3 NaH/THF

R

4 N NH2 R 4 N NH 2 heat A-1 B-1 Cl R5

R

5 -OH 4 N

R

2 DIEA/dioxane [I 2- R N S DMF or DMSO R 4 N N S R C-1 H as co-solvent H C-2 heat SCHEME D OH CI N \ 2 POC13g N heat N CI A-3 - 1NaH[THF

R

4 N NH2 R N NH 2 heat A-1 B1 CI R 5

-NH

2 R NH N>N DIEA N > R4 N R2 n-BuOH R N 2 R4 N S Heat R4 N S 5 C-i H H D-1 ASSAYS The compounds of the instant invention described in the Examples were tested by Assays I through IV described below and were found to have kinase inhibitory activity. 10 Compounds disclosed as dual inhibitors showed activity in the Cdk Assay, Assay V. Other assays are known in the literature and could be readily performed by those of skill in the art (see, -43 - WO 2004/041164 PCT/US2003/034100 for example, Dhanabal et al., Cancer Res. 59:189-197; Xin et al., J. Biol. Chem. 274:9116-9121; Sheu et al., Anticancer Res. 18:4435-4441; Ausprunk et al., Dev. Biol. 38:237-248; Gimbrone et al., J. Nati. Cancer Inst. 52:413-427; Nicosia et al., In Vitro 18:538-549). 5 I. VEGF RECEPTOR KINASE ASSAY VEGF receptor kinase activity is measured by incorporation of radio-labeled phosphate into polyglutamic acid, tyrosine, 4:1 (pEY) substrate. The phosphorylated pEY product is trapped onto a filter membrane and the incorporation of radio-labeled phosphate quantified by scintillation counting. 10 MATERIALS VEGF Receptor Kinase The intracellular tyrosine kinase domains of human KDR (Terman, B.I. et al. Oncogene (1991) vol. 6, pp. 1677-1683.) and Flt-i (Shibuya, M. et al. Oncogene (1990) vol. 5, 15 pp. 519-524) were cloned as glutathione S-transferase (GST) gene fusion proteins. This was accomplished by cloning the cytoplasmic domain of the KDR kinase as an in frame fusion at the carboxy terminus of the GST gene. Soluble recombinant GST-kinase domain fusion proteins were expressed in Spodoptera frugiperda (Sf21) insect cells (Invitrogen) using a baculovirus expression vector (pAcG2T, Pharmingen). 20 The other materials used and their compositions were as follows: Lysis buffer: 50 mM Tris pH 7.4, 0.5 M NaCl, 5 mM DTT, 1 mM EDTA, 0.5% triton X-100, 10% glycerol, 10 pg/mL of each leupeptin, pepstatin and aprotinin and 1 mM phenylmethylsulfonyl fluoride (all Sigma). 25 Wash buffdr: 50 mM Tris pH 7.4,0.5 M NaCl, 5 mM DTT, 1 mM EDTA, 0.05% triton X-100, 10% glycerol, 10 pg/mL of each leupeptin, pepstatin and aprotinin and 1 mM phenylmethylsulfonyl fluoride. 30 Dialysis buffer: 50 mM Tris pH 7.4,0.5 M NaCl, 5 mM DTT, 1 mM EDTA, 0.05% triton X 100, 50% glycerol, 10 pig/mL of each leupeptin, pepstatin and aprotinin and 1 mM phenylmethylsulfonyl fluoride. 10 X reaction buffer: 200 mM Tris, pH 7.4, 1.0 M NaCl, 50 mM MnCl2, 10 mM DTT and 5 35 mg/mL bovine serum albumin (Sigma). -44- WO 2004/041164 PCT/US2003/034100 Enzyme dilution buffer: 50 mM Tris, pH 7.4, 0.1 M NaCl, 1 mM DTT, 10% glycerol, 100 mg/mL BSA. 10 X Substrate: 750 pg/mL poly (glutamic acid, tyrosine; 4:1) (Sigma). 5 Stop solution: 30% trichloroacetic acid, 0.2 M sodium pyrophosphate (both Fisher). Wash solution: 15% trichloroacetic acid, 0.2 M sodium pyrophosphate. 10 Filter plates: Millipore #MAFC NOB, GF/C glass fiber 96 well plate. METHOD A. Protein purification 15 1. Sf21 cells were infected with recombinant virus at a multiplicity of infection of 5 virus particles/ cell and grown at 27"C for 48 hours. 2. All steps were performed at 4"C. Infected cells were harvested by centrifugation at 1000 X g and lysed at 4C for 30 minutes with 1/10 volume of lysis buffer followed by centrifugation at 100,00OXg for 1 hour. The supernatant was then passed over a 20 glutathione Sepharose column (Pharmacia) equilibrated in lysis buffer and washed with 5 volumes of the same buffer followed by 5 volumes of wash buffer. Recombinant GST-KDR protein was eluted with wash buffer/10 mM reduced glutathione (Sigma) and dialyzed against dialysis buffer. 25 B. VEGF receptor kinase assay 1. Add 5 tL of inhibitor or control to the assay in 50% DMSO. 2. Add 35 tL of reaction mix containing 5 AL of 10 X reaction buffer, 5 yL 25 mM ATP/10 pCi [ 3 3 P]ATP (Amersham), and 5 yL 10 X substrate. 3. Start the reaction by the addition of 10 pL of KDR (25 nM) in enzyme dilution 30 buffer. 4. Mix and incubate at room temperature for 15 minutes. 5. Stop by the addition of 50 yL stop solution. 6. Incubate for 15 minutes at 4*C. 7. Transfer a 90 yL aliquot to filter plate. 35 8. Aspirate and wash 3 times with wash solution. -45 - WO 2004/041164 PCT/US2003/034100 9. Add 30 ItL of scintillation cocktail, seal plate and count in a Wallac Microbeta scintillation counter. II. HUMAN UMBILICAL VEIN ENDOTHELIAL CELL MITOGENESIS ASSAY 5 Human umbilical vein endothelial cells (HUVECs) in culture proliferate in response to VEGF treatment and can be used as an assay system to quantify the effects of KDR kinase inhibitors on VEGF stimulation. In the assay described, quiescent HIUVEC monolayers are treated with vehicle or test compound 2 hours prior to addition of VEGF or basic fibroblast growth factor (bFGF). The mitogenic response to VEGF or bFGF is determined by measuring 10 the incorporation of [3H] thymidine into cellular DNA. MATERIALS HUVECs: HUVECs frozen as primary culture isolates are obtained from Clonetics Corp. Cells 15 are maintained in Endothelial Growth Medium (EGM; Clonetics) and are used for mitogenic assays described in passages 1-5 below. Culture Plates: NUNCLON 96-well polystyrene tissue culture plates (NUNC #167008). 20 Assay Medium: Dulbecco's modification of Eagle's medium containing 1 mg/mL glucose (low glucose DMEM; Mediatech) plus 10% (v/v) fetal bovine serum (Clonetics). Test Compounds: Working stocks of test compounds are diluted serially in 100% dimethylsulfoxide (DMSO) to 400-fold greater than their desired final concentrations. Final 25 dilutions to 1X concentration are made directly into Assay Medium immediately prior to addition to cells. lOX Growth Factors: Solutions of human VEGF165 (500 ng/mL; R&D Systems) and bFGF (10 ng/mL; R&D Systems) are prepared in Assay Medium. 30 lOX [ 3 H1Thymidine: [Methyl- 3 H]thymidine (20 Ci/mmol; Dupont-NEN) is diluted to 80 pCi/mL in low-glucose DMEM. Cell Wash Medium: Hank's balanced salt solution (Mediatech) containing 1 mg/mL bovine 35 serum albumin (Boehringer-Mannheim). - 46 - WO 2004/041164 PCT/US2003/034100 Cell Lysis Solution: 1 N NaOH, 2% (w/v) Na2CO3. METHOD 1. HUVEC monolayers maintained in EGM are harvested by trypsinization and 5 plated at a density of 4000 cells per 100 pL Assay Medium per well in 96-well plates. Cells are growth-arrested for 24 hours at 37"C in a humidified atmosphere containing 5% C02. 2. Growth-arrest medium is replaced by 100 pL Assay Medium containing either vehicle (0.25% [v/v] DMSO) or the desired final concentration of test compound. All determinations are performed in triplicate. Cells are then incubated at 37"C with 5% C02 for 2 10 hours to allow test compounds to enter cells. 3. After the 2-hour pretreatment period, cells are stimulated by addition of 10 pL/well of either Assay Medium, loX VEGF solution or lOX bFGF solution. Cells are then incubated at 37"C and 5% C02. 4. After 24 hours in the presence of growth factors, loX [ 3 H] thymidine (10 15 L/well) is added. 5. Three days after addition of [ 3 H]thymidine, medium is removed by aspiration, and cells are washed twice with Cell Wash Medium (400 pL/well followed by 200 L/well). The washed, adherent cells are then solubilized by addition of Cell Lysis Solution (100 pL/well) and warming to 37"C for 30 minutes. Cell lysates are transferred to 7-mL glass scintillation vials 20 containing 150 pL of water. Scintillation cocktail (5 mL/vial) is added, and cell-associated radioactivity is determined by liquid scintillation spectroscopy. Based upon the foregoing assays the compounds of the present invention are inhibitors of VEGF and thus are useful for the inhibition of angiogenesis, such as in the treatment of ocular disease, e.g., diabetic retinopathy and in the treatment of cancers, e.g., solid 25 tumors. The instant compounds inhibit VEGF-stimulated mitogenesis of human vascular endothelial cells in culture with IC50 values between 0.01 - 5.0 pM. These compounds may also show selectivity over related tyrosine kinases (e.g., FGFR1 and the Src family; for relationship between Src kinases and VEGFR kinases, see Eliceiri et al., Molecular Cell, Vol. 4, pp.915-924, December 1999). 30 III. FLT-1 KINASE ASSAY Flt-1 was expressed as a GST fusion to the Flt-1 kinase domain and was expressed in baculovirus/insect cells. The following protocol was employed to assay compounds for Flt-1 kinase inhibitory activity: 35 -47 - WO 2004/041164 PCT/US2003/034100 1. Inhibitors were diluted to account for the final dilution in the assay, 1:20. 2. The appropriate amount of reaction mix was prepared at room temperature: loX Buffer (20 mM Tris pH 7.4/0.1 M NaCl/1 mM DTT final) 0.1M MnCl2 (5 mM final) 5 pEY substrate (75 pg/mL)

ATP/[

3 3 P]ATP (2.5 pM/1 pCi final) BSA (500 ptg/mL final). 3. 5 gL of the diluted inhibitor was added to the reaction mix. (Final volume of 5 pL in 50% DMSO). To the positive control wells, blank DMSO (50%) was added. 10 4. 35 pL of the reaction mix was added to each well of a 96 well plate. 5. Enzyme was diluted into enzyme dilution buffer (kept at 4"C). 6. 10 pL of the diluted enzyme was added to each well and mix (5 nM final). To the negative control wells, 10 pL 0.5 M EDTA was added per well instead (final 100 mM). 15 7. Incubation was then carried out at room temperature for 30 minutes. 8. Stopped by the addition of an equal volume (50 pL) of 30% TCA/0.1M Na pyrophosphate. 9. Incubation was then carried out for 15 minutes to allow precipitation. 10. Transfered to Millipore filter plate. 20 11. Washed 3X with 15% TCA/0.1M Na pyrophosphate (125 pL per wash). 12. Allowed to dry under vacuum for 2-3 minutes. 13. Dryed in hood for - 20 minutes. 14. Assembled Wallac Millipore adapter and added 50 pL of scintillant to each well and counted. 25 IV. FLT-3 KINASE ASSAY Flt-3 was expressed as a GST fusion to the Flt-3 kinase domain, and was expressed in baculovirus/insect cells. The following protocol was employed to assay compounds for Flt-3 kinase inhibitory activity: 30 1. Dilute inhibitors (account for the final dilution into the assay, 1:20) 2. Prepare the appropriate amount of reaction mix at room temperature. lOX Buffer (20 mM Tris pH 7.4/0.1 M NaCl/1 mM DTT final) 0.1M MnCl 2 (5 mM final) 35 pEY substrate (75 pg/nL) - 48 - WO 2004/041164 PCT/US2003/034100 ATP/[P]ATP (0.5 [M/L [tCi final) BSA (500 ptg/mL final) 3. Add 5 pL of the diluted inhibitor to the reaction mix. (Final volume of 5 pL in 50% DMSO). Positive control wells - add blank DMSO (50%). 5 4. Add 35 pL of the reaction mix to each well of a 96 well plate. 5. Dilute enzyme into enzyme dilution buffer (keep at 4*C). 6. Add 10 pL of the diluted enzyme to each well and mix (5-10 nM final). Negative control wells - add 10 pL 0.5 M EDTA per well instead (final 100 mM) 7. Incubate at room temperature for 60 minutes. 10 8. Stop by the addition of an equal volume (50 pL) of 30% TCA/O.1M Na pyrophosphate. 9. Incubate for 15 minutes to allow precipitation. 10. Transfer to Millipore filter plate. 11. Wash 3X with 15% TCA/0.1M Na pyrophosphate (125 VL per wash). 15 12. Allow to dry under vacuum for 2-3 minutes. 13. Dry in hood for - 20 minutes. 14. Assemble Wallac Millipore adapter and add 50 pL of scintillant to each well and count. V. CYCLING DEPENDENT KINASE ASSAYS 20 CDK4 ASSAY Human cyclin D2-glutathione-S-transferase fused Cdk4 (GST-Cdk4) complexes were produced in Sf9 cells that had been co-infected with recombinant baculoviruses encoding cDNAs for cyclin D2 and GST-Cdk4. Cyclin D2-GST-Cdk4 complex was absorbed to 25 glutathione-sepharose 4B (Pharmacia Biotech) and eluted by digestion by precision protease. Active cycin D2-Cdk4 complexes were further purified by HPLC on Mono Q. Cyclin D2-Cdk4 assays for determinations of the IC50 values were performed in 96 well PS1-paper filter plates (Millipore). 1. Purified cyclin D2-Cdk4 was incubated with 50 pLM ATP, 1 [,Ci [ 3 3 P]ATP and 30 100 pM G1 peptide (RPPTLSPIPHIPR) in R buffer (20 VL of reaction buffer containing 20 mM Tris-HCI pH 7.4, 10 mM MgCl2, 4.5 mM 2-mercaptoethanol and 1 mM EGTA) for 45 minutes at 30'C. G1 peptide is identical to 775-787 amino acid residues of human retinoblastoma protein (pRb) and Ser site of pRb is specifically phosphorylated by Cdk4 (Kitagawa, M. et al, EMBO Journal, vol. 15, pp. 7060-7069, 1996). 35 2. Reaction was stopped by adding 10 pL of 350 mM H3PO4. -49- WO 2004/041164 PCT/US2003/034100 3. . Peptides were trapped on the 96 well filter plate and filters were washed with 75 mM H3PO4. 4. Radioactivity was determined by TOP count (Packard). 5 CDK2 ASSAY Human cyclin A-Cdk2 complexes were produced in Sf9 cells that had been co infected with recombinant baculoviruses encoding cDNAs for cyclin A and Cdk2. Active cyclin A-Cdk2 complexes were purified by BPLC on Mono Q, Hydroxyapatite and Mono S. Cyclin A-Cdk2 assays for determinations of the IC50 values were performed 10 according to the same method as cyclin D2-Cdk4 assay above. 1. Purified cyclin A-Cdk2 was incubated with 50 pM ATP, 0.5 piCi [ 3 3 P]ATP and 0.01 mg/mL of SI peptide (AKAKKTPKKAKK) in R buffer for 45 minutes at 30"C. SI peptide includes amino acid residues of human histone H1 (Kitagawa, M. et al, EMBO Journal, vol. 15, pp. 7060-7069, 1996). 15 2. Reaction was stopped by adding 10 pL of 350 mM H3P04. 3. Peptides were trapped on the 96 well filter plate and filters were washed with 75 mM H3PO4. 4. Radioactivity was determined by TOP count (Packard). 20 EXAMPLES Examples provided are intended to assist in a further understanding of the invention. Particular materials employed, species and conditions are intended to be further illustrative of the invention and not limiting of the reasonable scope thereof. 25 Some of the specific compounds exemplified herein are the protonated salts of amine compounds. The term "free form" refers to the amine compounds in non-salt form. The encompassed pharmaceutically acceptable salts not only include the salts exemplified for the specific compounds described herein, but also all the typical pharmaceutically acceptable salts of the free form of compounds of Formula I. The free form of the specific salt compounds 30 described may be isolated using techniques known in the art. For example, the free form may be regenerated by treating the salt with a suitable dilute aqueous base solution such as dilute aqueous NaOH, potassium carbonate, ammonia and sodium bicarbonate. The free form may differ from their respective salt forms in certain physical properties, such as solubility in polar solvents, but the acid and base salts are otherwise equivalent to their respective free forms for 35 purposes of the invention. - 50 - WO 2004/041164 PCT/US2003/034100 All such acid and base salts are intended to be pharmaceutically acceptable salts within the scope of the invention and all acid and base salts are considered equivalent to the free forms of the corresponding compounds for purposes of the invention. 5 SCHEME 1 O HO _N _OHDEAD N OH N / NH 2 Ph 3 P O CICN 11 N NH 2 NaH THF O HN 0 N TEA ,O

CH

2

CI

2 N N ON 1-2 N N N N S N N S 1-3 H H Synthesis of tert-butyl-4-[(6-aminopyrimidin-4-yl)oxylpiperidine-1-carboxylate (1-1) 4-Hydroxy-6-aminopyrimidine (554 mg, 5 mmol) was added to a solution tert butyl-4-hydroxy-piperidine-1-carboxylate (1.21 gm, 6 mmol) and triphenylphosphine (1.83 gm, 7 10 mmol) in dry THF (40 mL). This mixture was cooled in an ice bath and diethyldiazodicarboxlate (DEAD) (1.15 mL, 7 mmol) was added dropwise. The reaction was stirred at ice bath temperature for one hour and then gradually warmed to room temperature over two more hours. The solvent was removed under vacuum and the residue was chromatograghed on silica gel eluting with a 50-100% ethyl acetate/hexane gradient. The semipurified product was 15 rechromatograhed on silica gel eluting with a 1-3% methanol/chloroform gradient. This material was triturated with diethyl ether to give the title compound as a white crystalline solid, mp: 168 -51- WO 2004/041164 PCT/US2003/034100 172 0 C. 1 H-NMR (500 MHz, CDC13): 8.26 (1H,s), 5.80 (1H,s), 5.24 (1H,m), 5.08 (2H, m), 3.76 (2H,m), 3.27 (2H,m), 1.96 (2H,m), 1.71 (2H, m), 1.48(9H, s). M+1 = 295.3 Synthesis of tert-butyl-4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]pyriidin-4-yl}oxy) piperidine-1 5 carboxylate (1-2) Sodium hydride (60% in mineral oil, 95 mg, 2.4 mmol) was added to a solution of tert-butyl-4-[(6-aminopyrimidin-4-yl)oxy]piperidine-1-carboxylate (295 mg, 1 mmol) in dry THF (3 mL). After gas evolution ceased (-1/4 hr) a solution of 2-chloro-1,3-thiazole-5-carbonitrile (162 mg, 1.1 mmnol) dissolved in THF (2 mL) was added and the reaction was warmed to -50'C 10 for 22 hours. The cooled reaction was diluted with ethyl acetate and 2N HCl (0.7 mL) was added, followed by saturated NaHCO3 solution. The ethyl acetate exact was dried over anhydrous Na2SO4, filtered and evaporated. The residue was chromatographed on silica gel eluting with a 0-2% methanol/chloroform gradient. The appropriate fractions were combined and the solvents removed. The resultant residue was triturated with diethyl ether to give the title 15 compound as a white solid, mp: 224-227 0 C. 1H-NMR (500 MHz, CDCl3): 8.63(1H,s), 7.95(1H,s), 7.27(1H,s), 6.70(1H,br s), 5.36 (1H,m), 3.77(2H,m), 3.30 (2Hm), 2.05 (2H,m), 1.75 (2H,m), 1.48 (9H,s). M+1 = 403.2 Synthesis of 2-f [6-(piperidin-4-Vloxy)pyrimidin-4-yllamino }-1,3-thiazole-5-carbonitrile (1-3) 20 A solution of tert-butyl-4-({ 6-[(5-cyano-1,3-thiazol-2-yl)amino] pyrimidin-4 yl}oxy)piperidine-l-carboxylate (56 mg, 0.14 mmol) in methylene chloride (5 mL) containing TFA (0.5 mL) was stirred at room temperature over night. The solvents were evaporated and the residue was chased with chloroform (2X). The residue was dissolved in methanol, filtered through a plug of charcoal and the solvent nearly all removed under vacuum. This residue was 25 triturated with ethyl acetate to give the title compound as a crystalline TFA salt, mp: 240-242'C. 1 H-NMR (500 MHz, DMSO-d6): 8.68(1H,s), 8.33(1H,s), 6.41(1H,s), 5.33(lH,m), 3.26(2H,m), 3.13 (2H,m), 2.11(2H,m), 1.86(2H,m). M+1 = 303.2 Compounds 1-4 through 1-9 were synthesized via the same protocol shown in Scheme 1 by using an appropriate alcohol and 5-substituted 2-chlorothiazole. 30 Synthesis of tert-butyl-4-({6-[(5-phenyl-1,3-thiazol-2-yl)amino]pyrimidin-4-yl}oxy) piperidine 1-carboxylate (1-4) - 52- WO 2004/041164 PCT/US2003/034100 0 N N N S 1-4 H The title compound was prepared according to the protocol described in Scheme 1 except 2-chloro-5-phenyl-1,3-thiazole was substituted for 2-chloro-1,3-thiazole-5-carbonitrile. The product was obtained as a white solid, mp: 234-235'C. 5 l1H-NMR (500MHz, CDCI3): 8.60 (1H,s), 7.55 (3H, m), 7.46 (2H,t, J=8hz), 7.38 (1H, t, J=7Hz), 6.47 (1H,s), 5.31 (1H,m), 3.75 (2H,m), 3.30 (2H,m), 1.98 (2H,m), 1.75 (2H,m), 1.48 (9H,s). M+1 = 454.2 Synthesis of N-(5-phenyl-1,3-thiazol-2-y)-6-(piperidin-4-yloxy)pyrimidin-4-amine (1-5) HN N> N\ N N S 10 1-5 The title compound was prepared according to the procedure described in Scheme 1 using compound 1-4. This product was obtained as the TFA salt, mp: 241-243 0 C. lH-NMR (500 MHz, DMSO-d6): 8.61(1H,s), 8.51 (1H,br s), 8.43(1H, br s), 7.84 (1H,s), 7.60 (2H, d, J=8Hz), 7.41(2H, t, J=8 Hz), 7.29 (1H, t, J=7.1Hz), 6.43 (1H, br s), 5.31 (1H,m), 3.27 (2H, m), 15 3.14 (2H,m), 2.13 (2H,m), 1.89 (2H,m). M+1= 354.2 Synthesis of tert-butyl-4-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]pyriidin-4-yl oxy) methyl] piperidine-1-carboxylate (1-6) - 53 - WO 2004/041164 PCT/US2003/034100 0 0 N O CN H 1-6 The title compound was prepared according to the procedure described in Scheme 1 except tert-butyl-4-(hydroxymethyl)piperidine-1-carboxylate was substituted for tert-butyl-4 hydroxy-piperidine-1-carboxylate. The product was obtained as a white crystalline solid, mp: 5 228-229 0 C. 1H-NMR (500 MHz, CDC13): 8.63 (1H,s), 7.95 (1H,s), 7.25 (1H,s), 6.60 (1S, br s), 4.28(2H,d, J=6.4 Hz), 4.16 (2H, d, J=13.4 Hz), 2.74 (2H, t, J=12Hz), 1.79 (2H, d, J= 11.5 Hz), 1.46 (9H,s), 1.26 (2H,m). M+1 = 417.2 2-{ r6-(piperidin-4-ylmethoxy)pyrimidin-4-yllamino}-1,3-thiazole-5-carbonitrile (1-7) 0 HN N N S CN H 10 1-7 The title compound was prepared according to the procedure described in Scheme 1 using compound 1-6 above. This product was obtained as the TFA salt, mp: 244-245 0 C. iH NMR (500 MHz, DMSO-d6): 8.67(1H,s), 8.53(1H,br s), 8.32 (1H, s), 8.21(1H, br s), 6.40 (1H,s), 4.21 (2H, d, J=6.1Hz), 3.31 (2H, br d, J=13 Hz), 2.89 (21H, m), 2.07 (111, m), 1.88 (2H, 15 d, J=12.5Hz),1.45 (2H, q, J=12.5 Hz). M+1 = 317.2 tert-butyl-4-[({6-[(5-phenyl--1,3-thiazol-2-yl)amino]pyrimidin-4-yl}oxy)methyl]-piperidine-1 carboxylate (1-8) - 54 - WO 2004/041164 PCT/US2003/034100 0 0 N N N H 1-8 The title compound was prepared according to the procedure described in Scheme 1 except tert-butyl-4-(hydroxymethyl)piperidine-1-carboxylate was substituted for tert-butyl-4 hydroxy-piperidine-1-carboxylate and 2-chloro-5-phenyl-1,3-thiazole was substituted for 2 5 chloro-1,3-thiazole-5-carbonitrile, mp: 222-223 C. 1H--NMR (500MHz, CDCI3): 8.65 (1H,s), 7.45-7.55 (5H, in), 7.25 (1H,s), 6.61 (1H,s), 4.24(2H, d, J=6.6 Hz), 4.16 (2H,m), 2.74 (2H,br t, J=11Hz), 1.97 (1H,m), 1.78 (2H,d, J=13Hz), 1.47 (9H,s), 1.25 (2H, br m). M+1 = 468.2 10 N-(5-phenyl-1, 3 -thiazol- 2 -yl)-6-(piperidin-4-ylmethoxy)primidin-4-amine (1-9) 0 HN N N S 1-9 The title compound was prepared according to the procedure described in Scheme 1 using compound 1-8 above. This product was obtained as the TFA salt, mp: 238-239'C. 1H NMR (500 MHz, DMSO-d6): 8.60(1H,s), 8.53(1H,br s), 8.19 (1H, br s), 7.84(1H, s), 7.61(2H,d, 15 J=7.3Hz), 7.43(2H, t, J=7.8Hz), 7.29(1H, t, J=7.3Hz), 6.43 (1H,s), 4.23 (2H, d, J=6.4Hz), 3.31 (2H, br d, J=12 Hz), 2.92 (2H, q, J=10.5Hz), 2.17 (1H, m), 1.88 (2H, d, J=14Hz),1.45 (2H,q, J=10Hz). M+I1 = 368.2 - 55 - WO 2004/041164 PCT/US2003/034100 SCHEME 2 O CI /\-NOH NaH - NO\

NH

2 toluene N reflux 0 SOO O N -O \\CN --- CI N / NH 2 NaH 2-1 IN THF 0 HN O)<N HN o CH 2 Cl 2 N N O 2-2 N CN N N S N N S H 2-3 H Synthesis of tert-butyl-4-[(6-amino-2-methylpyrimidin-4-yl)oxylpiperidine-1-carboxylate (2-1) Solid 6-chloro-2-methylpyrimidin-4-amine (720 mg, 5 mmol), which is prepared 5 from 6-amino-2-methylpyrimidin-4-o1 as described in Chem. Ber., 75, 755(1942), was added to a solution of tert-butyl-4-hydroxy-piperidine-1-carboxylate (1.05 gm, 5.2 mmol) in dry toluene containing sodium hydride (60% in mineral oil, 238 mg, -6 mmol). The reaction mixture was heated at reflux for 18 hours. The cooled reaction was diluted with ethyl acetate, washed with saturated NaHCO3 and the solution was dried over anhydrous Na2SO4, filtered and the solvents 10 evaporated. The residue was purified by chromatography on silica gel eluting with a 40-70% ethyl acetate/hexane gradient. The appropriate fractions were combined, the solvent evaporated, and the residue triturated with diethyl ether to give the title compound as a white crystalline solid, mp: 142-145 0 C. 1H-NMR (500 MHz, CDCl3): 5.59 (1H,s), 5.21 (1H,m), 4.81 (2H, br s), 3.72 (2H,m), 3.28 (2H,m), 2.43 (3H,s), 1.93 (2H,m), 1.68 (2H,m), 1.46 (9H,s). M+1 = 309.2 15 Synthesis of tert-butyl-4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4 vloxy)piperidine-1-carboxylate (2-2) - 56 - WO 2004/041164 PCT/US2003/034100 Sodium hydride (60% in mineral oil, 121 mg, 3 mmol) was added to a solution of tert-butyl-4-[(6-amino-2-methylpyrimidin-4-yl)oxy]piperidine-1-carboxylate (360 mg, 1.17 mmol) in dry THF (5 mL). After gas evolution ceased (-1/4 hr) a solution of 2-chloro-1,3 thiazole-5-carbonitrile (189 mg, 1.3 mmol) dissolved in THF (2 mL) was added and the reaction 5 was warmed to -50'C for 22 hours. The reaction progress was monitored by LC/MS periodically adding additional sodium hydride (another 180 mg) and 2-chloro-1,3-thiazole-5-carbonitrile (another 286 mg) in portions until the reaction was complete. The cooled reaction was diluted with ethyl acetate and 2N HC1 (3 mL) was added, followed by saturated NaHCO3 solution. The ethyl acetate extract was dried over anhydrous Na2S04, filtered and evaporated. The residue was 10 purified by chromatography on silica gel eluting with a 15-40% ethyl acetate/hexane gradient. The appropriate fractions were combined and the solvents removed. The resultant residue was triturated with diethyl ether to give the title compound as a white solid, mp: 227-228'C. IH NMR (500 MHz, CDCl3): 9.90(lH, br s), 5.97 (1H,s), 5.34(1H,m), 3.78(2H,m), 3.29(2H,m), 2.62 (3H,s), 1.96(2H,m), 1.72(2H,m), 1.48(9H,s). M+1 = 417.2 15 Synthesis of 2- { [2-methyl-6-(piperidin-4-yloxy)pyrimidin-4-yl] amino 1-1,3-thiazole-5 carbonitrile (2-3) A solution of tert-butyl-4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2 methylpyrimidin-4-yl}oxy)piperidine-1-carboxylate (126 mg, 0.30 mmol) in chloroform (5 mL) 20 containing TFA (0.75 mL) was stirred at room temperature over night. The solvents were evaporated and the residue was chased with chloroform (2X). The residue was dissolved in a minimum amount of methanol and diluted with diethyl ether and the title compound crystallized out as the TFA salt, mp: >250"C. 1H-NMR (500 MHz, DMSO-d6): 8.45(2H, br s), 8.32(1H,s), 622(1H,s), 5.32(1H,m), 3.26(2H,m), 3.14 (2H,m), 2.56(3H, s), 2.11(2H,m), 1.85(2H,m). M+1 = 25 317.2 Compounds 2-4 through 2-7 below were synthesized via the same protocol shown in Scheme 2 by using the appropriate alcohol and 5-substituted 2-chlorothiazole. Synthesis of N-(5-phenyl-1,3-thiazol-2-yl)-6-(piperidin-4-yloxy)-2-methylpyrimidin-4 30 amine (2-4) - 57 - WO 2004/041164 PCT/US2003/034100 HNUO N NN S'O H 2-4 The title compound was prepared according to the procedure described in Scheme 2 substituting 2-chloro-5-phenyl-1,3-thiazole for 2-chloro-1,3-thiazole-5-carbonitrile. This product was obtained as the TFA salt, mp: 247-249 0 C. 1H-NMR (500 MHz, DMSO-d6): 5 8.52(1H,br s), 8.45(1H,br s), 7.82 (1H,s), 7.61 (2H, d, J=7.3Hz), 7.41(2H, t, J=7.6 Hz), 7.29 (1H, t, J=7.6Hz), 6.24 (11H, s), 5.31 (1H,m), 3.25 (2H, m), 3.15 (2H,m), 2.54(3H,s), 2.11 (2H,m), 1.87 (2H,m). M+1= 368.2 Synthesis of 2-({2-methyl-6-[(3R)-pyrrolidin-3-yloxypyrimidin-4-y I amino)- 1,3-thiazole-5 10 carbonitrile (2-5) 0 NH N C Al"N N S O H 2-5 The title compound was prepared according to the procedure described in Scheme 2 except tert-butyl-(3R)-3-hydroxypyrrolidine-1-carboxylate was substituted for tert-butyl-4 hydroxy-piperidine-1-carboxylate. This product was obtained as the TFA salt, mp: 221-223'C. 15 1H-NMR (500 MHz, DMSO-d6): 9.05(1H,br s), 8.90(1H,br s), 8.32 (1H,s), 6.24 (1H, s), 5.67 (1H,m), 3.48 (1H, m), 3.41 (1H,m), 3.33(2H, m), 2.58(3H,s), 2.26 (1H,m), 2.17 (1H,m). M+1= 304.2 Synthesis of 2-({2-methyl-6-[(3S)-pyrrolidin-3-yloxy]pyrimidin-4-yl }amino)-1,3-thiazole-5 20 carbonitrile (2-6) -58- WO 2004/041164 PCT/US2003/034100 (S) NH N N N S O H 2-6 The title compound was prepared according to the procedure described in Scheme 2 except tert-butyl-(3S)-3-hydroxypyrrolidine-1-carboxylate was substituted for tert-butyl-4 hydroxy-piperidine-1-carboxylate. This product was obtained as the TFA salt, mp: 222-224'C. 5 1H-NMR (500 MHz, DMSO-d6): 9.06 (1H,br s), 8.94(1H,br s), 8.32 (1H,s), 6.24 (1H, s), 5.67 (1H,m), 3.48 (1H, in), 3.41 (1H,m), 3.33(2H, m), 2.58(3H,s), 2.26 (1H,m), 2.17 (1H,m). M+1= 304.2 Synthesis of 2- {[2-methyl-6-(piperidin-4-ylmethoxy)pyrimidin-4-yl amino }-1 ,3-thiazole-5 10 carbonitrile (2-7) 0 HN N N N S N H 2-7 The title compound was prepared according to the procedure described Scheme 2 except tert-butyl-4-hydroxymethyl-piperidine-1-carboxylate was substituted for tert-butyl-4 hydroxy-piperidine-1-carboxylate. This product was obtained as the TFA salt. 1H-NMR (500 15 MHz, DMSO-d6): 8.57(1H,br s), 8.32 (1H, s), 8.22(1H, br s), 6.22 (1H,s), 4.22 (2H, d, J=6.3Hz), 3.30 (2H, m), 2.91 (2H, m), 2.56 (3H, s), 2.06 (1H, m), 1.91 (2H, d, J=13.9 Hz), 1.45 (21H, q, J=12.5 Hz). M+1 = 331.3 Synthesis of tert-Butyl 2-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4 20 yl}_oxy)methyll-morpholine-4-carboxylate (2-8) - 59 - WO 2004/041164 PCT/US2003/034100 0 0 N O ONN N N S N 2-8 H A solution of 1 M borane/THF (6 mL, 6 mmol) was added dropwise to a solution of racemic 4-(tert-butoxycarbonyl)morpholine-2-carboxylic acid (693 mg, 3 mmol) in THF (6 mL) which was cooled in a ice bath. Let stir at ambient temperature over night and then 5 quenched reaction by careful addition of methanol (3 mL). Saturated aqueous sodium sulfate (-1 mL) was added to this reaction, followed by solid sodium sulfate. The precipitate was removed by filtration and the organic solution was evaporated under reduced pressure. The residue was diluted with methanol and the solvent evaporated (2X). To remove traces of water, the residue was dissolved in toluene and the solvent removed to give racemic tert-butyl 2 10 (hydroxymethyl)morpholine-4-carboxylate as a viscous oil which was used as is. The title compound was prepared according to the procedure described in Scheme 2 except racemic tert-butyl 2-(hydroxymethyl)morpholine-4-carboxylate was substituted for tert butyl 4-hydroxy-piperidine-1-carboxylate. The racemic product was obtained as a white crystalline solid, mp: 233-235 C. lH-NMR (500 MHz, CDC1 3 ): 9.55 (1H, br s), 7.96 (1H,s), 15 7.26 (1H,s), 4.44 (2H,d, J=4.6 Hz), 4.06 (1H, br s), 3.95 (2H, d, J=10.2 Hz), 3.88 (1H, br s), 3.79 (1H, br s), 3.59 (1H, t, J=11Hz), 3.01 (1H, br s), 2.82 (1H, br s), 2.63 (3H, s), 1.48 (9H,s). M+1 = 433.4 Synthesis of 2-{ [ 2 -Methyl-6-(morpholin-2-ylmethoxy)pyrimidin-4-yl]amino }-1,3-thiazole-5 20 carbonitrile (2-9) HN 0 N N I~i / ON N N S H 2-9 The title compound was prepared according to the procedure described in Scheme 2 using compound 2-8 above. This racemic product was obtained as the TFA salt, mp: 241 - 60 - WO 2004/041164 PCT/US2003/034100 242 0 C. 1H-NMR (500 MHz, DMSO-d6): 12.3(1H, br s), 8.93(1H,br s), 8.32(1H, s), 6.24 (1H,s), 4.39 (2H, m), 4.01(2H, dd, J=3 Hz, J=12 Hz), 3.72 (1H, t, J=11 Hz), 3.33 (iH, d, J=3 Hz), 3.21 1H, d, J=3 Hz), 2.94-3.06 (2H, m), 2.56 (3H,s). M+1 = 333.4 5 Synthesis of 2-{[2-Methyl-6-(tetrahydro-2-pyran-4-yloxy)pyrimidin-4-yl] amino}-1,3-thiazole-5 carbonitrile (2-10) OaO N I -C N N S C H 2-10 The title compound was prepared according to the procedure described in Scheme 2 except tetra-hydro-2H-pyran-4-ol was substituted for tert -butyl 4-hydroxy-piperidine-1 10 carboxylate. This product was obtained as the TFA salt, mp: >260 C. 1H-NMR (500 MHz, DMSO-d6): 12.16(1H,br s), 8.30 (1H,s), 6.19 (1H, s), 5.25 (1H,m), 3.84 (2H, m), 3.51 (2H,t, J=11.7 Hz), 2.54 (3H, s), 1.98 (2H,m), 1.64 (2H,m). M+1= 318.3 Synthesis of 2-{ [2-Isopropyl-6-(piperidin-4-yloxy)pyrimidin-4-yl] amino -1,3-thiazole-5 15 carbonitrile (2-11) H N N CN N N S H 2-11 A mixture of 2-isopropyl-4,6-dichloropyrimidine (1.3 gm, 6.8 mmol), which is obtained according to the procedures described in Aust. J. Chem., 30, 1785-91(1977), in conc. aq. ammonium hydroxide (10 mL) and n-butanol (10 mL) was sealed in a screw-top vessel and -61- WO 2004/041164 PCT/US2003/034100 heated at 90"C for four hours. The cooled mixture was extracted into ethyl acetate, washed with water and the organic layer dried over anhydrous sodium sulfate. The filtered solution was evaporated to a small volume and diluted with diethyl ether as the product, 6-chloro-2 isopropylpyrimidin-4-amine, crystallized out, mp: 194-196 0 C. 1H-NMR (500 MHz, CDCl 3 ): 5 6.28 (1H, s), 4.90 (2H, br s), 3.96 (1H,m), 1.27 (6H, d, J=6.8 Hz). The title compound was prepared according to the procedure described in Scheme 2 except 6-chloro-2-isopropylpyrimidin-4-amine was substituted for 6-chloro-2 methylpyrimidin-4-amine. This product was obtained as the TFA salt, mp: 234-235"C. 1H-NMR (500 MHz, DMSO-d6): 12.21 (1H, s), 8.54 (1H, br s), 8.48(1H, br s), 8.32(1H,s), 6.23 (1H,s), 10 5.32(IH,m), 3.46(2H,m), 3.15 (2H,m), 3.05 (1H, m), 2.13(2H,m), 1.89 (2H,m), 1.34 (6H, d, J=6.8 Hz). M+1= 345.4 SCHEME 3 N 0 TFA HN O O0 N

-

CHC13 1 - CHC 3-1 N / NH 2 .2TFA N NH 2 N 2-1 N 0 0 N N N C Et 3 N

CH

3 CN 3-2 N 0 N 2N CI S0 NaH 3-3 N C OO THE N N S H -62- WO 2004/041164 PCT/US2003/034100 Synthesis of 2-methyl-6-(piperidin-4-yloxy)pyrimidin-4-amine (3-1) A solution of tert-butyl-4-[(6-amino-2-methylpyrimidin-4-yl)oxy] piperidine-1 carboxylate (2-1) (200 mg, 0.65 mmol) in chloroform (5 mL) containing TFA (0.25 mL) was stirred at room temperature for three hours. The solvents were evaporated and the residue was 5 chased with chloroform (2X). The residue was triturated with diethyl ether and allowed to stir over night. The title compound was collected and determined to be the bis-TFA salt. This material was used as is. 1H-NMR (500 MHz, DMSO-d6): 8.55 (1H, br s), 8.50 (11H, br s), 7.34 (111, br s), 5.73 (111, s), 5.18 (111, m), 3.22 (2H, m), 3.12 (21H, m), 2.34 (3H,s), 2.08 (2H,m), 1.83 (2H, m). 10 Synthesis of 2-methyl-6-f rl-(2-morpholin-4-ylethyl)piperidin-4-Vlloxylpyrimidin-4-amine (3-2) A clear solution was obtained when triethylamine (0.45 mL, 3.2 mmol) was added to a suspension of 2-methyl-6-(piperidin-4-yloxy)pyrimidin-4-amine bis-TFA salt (264 mg, 0.60 mmol) in acetonitrile (5 mL). Then 4-(2-chloroethyl) morpholine hydrochloride (122 mg, 0.65 15 mmol) was added to the reaction and this solution was heated at 80'C over night. The cooled mixture was evaporated to remove the solvent and the residue was purified by chromatography on silica gel eluting with a 1-8% methanol/ethyl acetate gradient. The appropriate fractions were combined and the solvent removed under vacuum. This residue was triturated with diethyl ether to give solid product. This material was used as is. 1H-NMR (500 MHz, CDCl3): 5.58 (1H, s), 20 5.03 (1H, m), 4.06 (2H, br s), 3.72 (4H, m), 2.77(2H, m), 2.54 (4H, br s), 2.50 (4Hbr s), 2.41 (3H,s), 2.35 (2H, br t, J=8.6Hz), 2.01 (2H,m), 1.79 (2H, m). M+1 = 322.3 Synthesis of 2-[(2-methyl-6-{[1-(2-morpholin-4-ylethyl)piperidin-4-yl]oxy} pyrimidin-4 yl)aminol-1,3-thiazole-5-carbonitrile (3-3) 25 The title compound was prepared according to the procedure described in Scheme 2 for compound 2-2, except 2-methyl-6-{[1-(2-morpholin-4-ylethyl) piperidin-4 yl]oxy}pyrimidin-4-amine was substituted for tert-butyl-4-[(6-amino-2-methylpyrimidin-4 yl)oxy]piperidine-1-carboxylate. The pure product was obtained after chromatography on silica gel (eluting with a 1-10% methanol/ chloroform saturated with ammonium hydroxide) and 30 crystallization from methanol, mp: 238-240 0 C. 1H-NMR (500 MHz, DMSO-d6): 8.80(1H,br s), 7.93 (1H,s), 5.94 (1H, s), 5.17 (1H, m), 3.72 (4H,m), 2.79 (21H, m), 2.61 (3H, s), 2.55 (411, br s) 2.50 (4H,br s), 2.37 (2H, br t, J=8.6Hz), 2.02 (2H,m), 1.82 (2H, m). M+1= 430.2 - 63 - WO 2004/041164 PCT/US2003/034100 SCHEME 4 TFA. H N O O Br 0 2-3 N N ex DIEA N N S CN

CH

3 CN H N TFA O0 CHC1 3 4-1 N N N N S H HO 0 O NH 2 4- ~ N ON EDO 4- I-C HOBT /Et 3 N N N C DMF H H N N O 0 N N C 4-3 N O ,N IN" S H Synthesis of tert-butyl-[4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4 Vloxy)piperidin-1-yllacetate (4-1) -64- WO 2004/041164 PCT/US2003/034100 Diisopropylethylamine (0.21 mL, 1.2 mmol) was added to a suspension of 2-{[2 methyl-6-(piperidin-4-yloxy)pyrimidin-4-yl]amino }-1,3-thiazole-5-carbonitrile TFA salt (2-3) (165 mg, 0.38 mmol) in dry acetonitrile(4 mL) to give a clear solution. Tert-butyl bromoacetate (0.07 mL, 0.45 mmol) was then added and the solution was heated to 80'C for 1.25 hours. Upon 5 cooling to room temperature the product began to crystallize out. Most of the title compound was obtained by filtration from the reaction mixture. Additional material was recovered from the mother liquors by chromatography on silica gel and elution with a 40-60% ethyl acetate/hexane gradient. This material was used as is. 1H-NMR (500 MHz, CDCI3): 9.50 (1H, br s), 7.95(1H, s), 5.96 (1H, s), 5.20 (1H, m), 3.17 (2H, s), 2.85 (2H, m), 2.61 (3H, s), 2.52 (211, br t, J=1OHz), 10 2.05 (2H, m), 1.88 (211, m), 1.48 (911, s). M+1 = 431.3 Synthesis of [4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-yl}oxy) piperidin-1 ylacetic acid (4-2) A solution of tert-butyl-[4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2 15 methylpyrimidin-4-ylIoxy)piperidin-1-yl] acetate (107 mg, 0.25 mmol) in chloroform (5 mL) containing TFA (1 mL) was stirred at room temperature over night. The solvent was evaporated and the residue chased with chloroform (2X). Then upon trituration with diethyl ether the title compound was obtained as a solid TFA salt. This material was used as is. 1H-NMR (500 MHz, DMSO-d6): 8.31 (1H, s), 6.21 (1H, s), 5.25 (1H, m), 3.76 (2H, br s), 3.20 (2H, br s), 3.07 (21H, br 20 s), 2.55 (3H, s), 2.12 (2H, br s), 1.93 (2H, br s). M+1 = 375.1 Synthesis of 2-[4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-yl} oxy)piperidin-1-yll-N-isopropylacetamide (4-3) To a suspension of [4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrinidin 25 4-yl}oxy)- piperidin-1-yl]acetic acid TFA salt (107 mg, 0.21 mmol) in dry DMF (2 mL) was added triethylamine (0.085 mL, 0.60 mmol), isopropyl amine (0.21 mL, 0.23 mmol), 1 hydoxybenzotriazole hydrate (31 mg, 0.3 mmol) and finally 1-ethyl-3-(3 dimethylaminopropyl)carbodiimide hydrochloride (EDC)(51 mg, 0.26 mmol). This reaction mixture was stirred at room temperature over night. The reaction was diluted with ethyl acetate, 30 washed with water, and the organic layer dried over anhydrous Na2S04, filtered and the solvent removed under reduced pressure. The residue was triturated with diethyl ether and the title compound slowly crystallized out of solution. This semi-pure material was dissolved in chloroform and some TFA was added to form the TFA salt. Upon evaporation of the solvent and trituration of the residue with diethyl ether the TFA salt was obtained as a white solid, mp: 226 35 228 0 C. -65 - WO 2004/041164 PCT/US2003/034100 1H-NMR (500 MHz, CDCl3): 7.97(1H, s), 7.01(1H, d, J= 8 Hz), 6.00(1H, s), 5.22(1H, m), 4.12(1H, m), 3.02(2H, s), 2.77(2H, m), 2.62(3H, s), 2.46(2H, m), 2.04(2H, br s), 1.84(2H, br s), 1.18(2H, d, J=6.4Hz). M+1 = 416.2 Compounds 4-4 through 4-6 below were synthesized via the same protocol shown 5 in Scheme 4 by using the appropriately substituted piperidine and an amine. Synthesis of Tert-Butyl{4-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4 yl I oxy)methyllpiperidin- 1-yl I acetate (4-4) 0 0 N o N N N N S N H 4-4 10 The title compound was prepared according to the procedure described in Scheme 4 except 2-{ [2-methyl-6-(piperidin-4-ylmethoxy)pyrimidin-4-yl] amino }- 1,3-thiazole-5 carbonitrile TFA salt (2-7) was substituted for 2-{[2-methyl-6-(piperidin-4-yloxy)pyrimidin-4 yl]amino}-1,3-thiazole-5-carbonitrile TFA salt (2-3). This product was obtained as the freebase, mp: 210-212*C. 1H-NMR (500 MHz, CDCl3): 9.45(1H,br s), 7.95(1H, s), 5.95 (1H,s), 4.21 (2H, 15 d, J=5.9Hz), 3.31 (211, s), 3.00 (2H, d, J=11.2 Hz),2.62 (3H, s), 2.20 (2H, t, J=11.2 Hz), 1.79 (3H, d, J=11.2 Hz), 1.50 (2H,m),1.47 (911, s). M+1 = 445.4 Synthesis of {4-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-yl}oxy) methyllpiperidin-1-yl I acetic acid (4-5) 0 0 NO HO N CN H 20 4-5 The title compound was prepared according to the procedure described in Scheme 4 from tert-butyl {4-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4 yl }oxy)methyl]piperidin-1-yl } acetate (4-4) above. This product was obtained as the zwitterionic - 66 - WO 2004/041164 PCT/US2003/034100 freebase, mp: >250"C. lH-NMR (500 MHz, DMSO-d6): 12.19(1H,br s), 8.31(1H, s), 6.21 (1H,s), 4.18 (2H, d, J=6.3Hz), 3.21 (4H, m), 2.58 (2H, m), 2.56 (3H, s), 1.86 (1H, m), 1.78 (211, d, J=13.2 Hz), 1.47 (21H, m). M+1 = 388.3 (very weak), 156.2 (M -233) 5 Synthesis of N-(tert-Butyl)-2-{4-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4 Vloxylmethyl1piperidin-1-vl acetamide (4-6) 0 0 N -N H N N N~ CN N N- S C H 4-6 The title compound was prepared according to the procedure described in Scheme 4 except {4-[({ 6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4 10 yl}oxy)methyl]piperidin-1-yl)acetic acid (4-5) above was substituted for [4-({6-[(5-cyano-1,3 thiazol-2-yl)amino]-2-methylpyrimidin- 4 -yloxy)- piperidin-1-yl] acetic acid TFA salt (4-2) and tert-butyl amine was substituted for isopropyl amine. This product was obtained as the freebase, mp: 248-249'C. 1H-NMR (500 MHz, CDC13): 12.16(1H,br s), 8.29(1H, s), 7.14 (1H, br s), 6.19 (1H,s), 4.15 (2H, d, J=6.lHz), 3.32 (4H, s), 2.54 (3H, s), 2.07 (2H, t, J=10.5 Hz), 1.71 (2H, d, 15 J=11.5 Hz), 1.29 (2H, m),1.27 (9H, s). M+1 = 444.4 - 67 - WO 2004/041164 PCT/US2003/034100 SCHEME 5 0 N OEt LiAIH 4 N OH Et 2 O 5-1 CI NN O N NH 2 O N NaH O-xylene N NH 2 N 0 N\ I CN NN\ CI S 5-2 CN NaH N N S Dioxane H Synthesis of 3-morpholin-4-ylpropan-1-ol (5-1) Ethyl 3-morpholin-4-ylpropanoate (2.0 gm, 10.68 mmol) was dissolved in ether 5 (200 mL). A 1 M solution of lithium alumumhydride in ether (11.0 mL, 11.0 mmol) was added dropwise and the reaction allowed to stir for 2.5 hours. Saturated sodium sulfate was added and the reaction allowed to stir over night. Solid sodium sulfate was then added and the reaction filtered. Solvent removal yielded 3-morpholin-4-ylpropan-1-ol. 1H-NMR (500 MHz, CDCl3): 5.00(1H,br s), 3.82(2H, t, J=5.37hz), 3.71(4H, t, J=4.39), 2.62(4H, t, J=5.86hz), 2.53(4H, br s), 10 1.74(2H, m). M+1=146 Synthesis of 2-{ [2-methyl-6-(3-morpholin-4-ylpropoxy)pyrimidin-4-yl]amino }-1 ,3-thiazole-5 carbonitrile (5-2) Sodium hydride (60% in mineral oil, 34.5 mg, 0.86 mmol) was added to a mixture 15 of o-xylene (3.0 mL) and 3-morpholin-4-ylpropan-1-ol (100 mg, 0.69 mmol). After the effervescence had stopped the 6-chloro-2-methylpyrimidin-4-amine (98.9 mg, 0.69 mmol) was added and the reaction heated at 130'C over night. The excess o-xylene was removed under vacuum and the residue dissolved into dry dioxane and sodium hydride (60% in mineral oil, 34.5 mg, 0.86 mmol) was then added. After the effervescence had stopped 2-chloro-1,3-thiazole-5 20 carbonitrile (99.6 mg, 0.69 mmol) was added and the reaction heated at 100'C over night. The -68- WO 2004/041164 PCT/US2003/034100 dioxane was removed and the residue treated with trifluoroacetic acid (2 drops) and methanol (4.0 mL). This was then purified using preparative HPLC to give 2-{[2-methyl-6-(3-morpholin 4-ylpropoxy)pyrimidin-4-yl]amino}-1,3-thiazole-5-carbonitrile as the trifluoroacetic acid salt. IH-NMR (500 MHz, DMSO-d6): 12.40 (1H, br s), 9.83 (1H, s), 8.31 (1H, s) 6.23 (111, s), 4.39 5 (2H, t, J=5.86hz), 4.00 (2H, d, J=12.45Hz), 3.65 (2H, t, J=11.96Hz), 3.48 (2H, d, J=12.70Hz), 3.27 (2H, br s), 3.09 (2H, br s), 2.56 (3H, s), 2.13 (2H, m). High res. ES MS: Theoretical Mass 361.1441, Measured Mass 61.1446 (C16H20N602S+H') Compounds 5-3 through 5-4 were synthesized via the same protocol shown in Scheme 5 by using an appropriate alcohol. 10 Synthesis of 2-{[2-methyl-6-(2-morpholin-4-ylethoxy)pyrimidin-4-yl] amino } -1,3-thiazole-5 carbonitrile (5-3) 0 N N CN H 5-3 The title compound was prepared according to scheme 5, except 2-morpholin-4 15 ylethanol was substituted for 3-morpholin-4-yl propan-1-ol to obtain 2-{[2-methyl-6-(2 morpholin-4-ylethoxy)pyrimidin-4-yl]amino}-1,3-thiazole-5-carbonitrile as the trifluoroacetic acid salt. 1H-NMR (500 MHz, CD30D): 8.04 (1H, s) 6.26 (11H, s), 4.78 (2H, t, J=5.13hz), 4.07 (211, br s), 3.56 (211, br s), 3.66 (2H, t, J=6.84Hz), 3.62 (2H, br s), 3.30 (2H, br s), 3.17 (3H, s). High res. ES MS: Theoretical Mass 347.1285, Measured Mass 347.1243 (C151118N602S+H*) 20 Synthesis of 2-{ [2-methyl-6-(2-piperidin- 1 -ylethoxy)pyrimidin-4-yl]amino -1,3-thiazole-5 carbonitrile (5-4) - 69 - WO 2004/041164 PCT/US2003/034100 N N CN N N S H 5-4 The title compound was prepared according to Scheme 5, except 2-piperidin-1 ylethanol was substituted for 3-morpholin-4-yl propan-1-ol to obtain 2-{[2-methyl-6-(2 piperidin-1-ylethoxy)pyrimidin-4-yl]amino } -1,3-thiazole-5-carbonitrile as the trifluoroacetic acid 5 salt. 1H-NMR (500 MHz, CD30D): 8.04 (11H, s) 6.25 (11H, s), 4.75 (2H, t, J=5.13hz), 3.66 (2H, d J=12.21Hz), 3.57 (2H, t, J=5.12Hz), 3.05 (2H, t, J=12.69Hz), 1.97 (2H, d, J=15.14Hz), 1.82 (31H, m), 1.54 (1H, m). M+1 = 345.0 SCHEME 6 CI N\ CI N S O N N N NH2 CIH CNNCN N N 2 THE N' S O 10 6-1 H Synthesis of 2-r(6-chloro-2-methylpyrimidin-4-yl)aminol-1,3-thiazole-5-carbonitrile (6-1) 6-Chloro-2-methylpyrimidin-4-amine (144.0 mg, 1.0 mmol), which is prepared from 6-amino-2 methylpyrimidin-4-ol as described in Chem. Ber., 75, 755(1942), was added to the reaction flask along with dioxane (3.0 mL), and sodium hydride (60% in mineral oil, 120 mg, 3.0 mmol). After 15 the effervescence had subsided 2-chloro-1,3-thiazole-5-carbonitrile (145.0 mg, 1.0 mmol) was added. After 2.0 hours the heat was removed and the reaction allowed to stir over night. The reaction was diluted with water then, acidified with concentrated HCl. The pH was adjusted to 8 with sodium bicarbonate and the aqueous layer extracted with ethyl acetate (3x1OOmL). The combined extracts were washed with brine (1x25mL) and dried (MgSO4). Solvent removal 20 yielded the desired 2-[(6-chloro-2-methyl-pyrimidin-4-yl)amino]-1,3-thiazole-5-carbonitrile. lH-NMR (500 MHz, DMSO-d6): 12.74 (1H,s), 8.38 (1H,s), 6.95 (1H, s), 2.62 (3H, s). M + 1=252 - 70 - WO 2004/041164 PCT/US2003/034100 SCHEME 7 0 CI O N N exNDIEA N N N CN o-xylene N N CN 6-1 H 7-1 H Synthesis of 2-({ 2-methyl-6-[(2-morpholin-4-ylethyl)amino]pyrimidin-4-yl amino)-1,3-thiazole 5-carbonitrile (7-1) 5 2-[(6-Chloro-2-methylpyrimidin-4-yl)amino]-1,3-thiazole-5-carbonitrile (6 1)(75.0 mg, 0.30 mmol) was added to a reaction flask along with o-xylene (3.0 mL), diisopropylethyl amine (154.1 mg, 1.19 mmol) and 4-(2-aminoethyl)morpholine (77.6 mg, 0.60 mmol). The reaction was heated over night at 120'C. The o-xylene was removed under high vacuum and the residue dissolved into methanol (4.0 mL) and purified by preparative HPLC. 10 This gave 2-({2-methyl-6-[(2-morpholin-4-ylethyl)amino]pyrimidin-4-yl } amino)-1,3-thiazole-5 carbonitrile as the trifluoroacetic acid salt. 1H-NMR (500 MHz, CD30D): 8.01 (1H, s) 6.01 (1H, s), 3.94 (4H, br s), 3.81(2H, t, J=5.86H1z), 3.46(4H, br s), 3.41(2H, t, J=5.6lHz), 2.56 (3H, s). M+1 = 346.0 15 SCHEME N N H CI1 0" 0, NN~ S NH2 N N exDIEA N N ON NN s CN n-BuOH 8-1 H 6-1 H Synthesis of 2-({6-[(3-morpholin-4-ylpropyl)amino]pyrirnidin-4-yl }anino)-1,3-thiazole-5 carbonitrile (8-1) 2-[(6-Chloro-2-methylpyrimidin-4-yl)amino]-1,3-thiazole-5-carbonitrile (6-1) 20 (75.0 mg, 0.298 mmol) was added to a reaction flask along with 3-morpholin-4-ylpropan-l amine (171.9 mg, 1.19 mmol), N,N-diisopropylethylamine (231 mg, 1.79 mmol), and N-butanol (3.0 mL). The reagents were heated over night at 120'C. The reaction was cooled and excess -71- WO 2004/041164 PCT/US2003/034100 solvents removed. The residue was then purified on a preparative HPLC. This gave the desired 2-({6-[(3-morpholin-4-ylpropyl)amino]-pyrimidin-4-yl} amino)-1,3-thiazole-5-carbonitrile as the trifluoroacetic acid salt. lH-NMR (500 MHz, DMSO-d6): 12.00 (1H, br s), 8.24 (1H, s), 7.50 (1H,br s) 5.90 (11H, br s), 3.98 (2H, br s), 3.66 (2H, br s), 3.43 (2H, br s), 3.34 (2H, br s), 3.16 5 (2H, br s), 3.07 (2H, br s), 2.41 (31H, s), 1.90 (2H, br s). M+1 = 346.0 Compounds 8-2 through 8-6 were synthesized via the same protocol shown in Scheme 5 by using an appropriate amine. Synthesis of 2-{[2-methyl-6-(tetrahydro-2H-pyran-4-ylamino)pyrimidin-4-yl] amino } -1,3 10 thiazole-5-carbonitrile (8-2) NH N CN

N

AN N ' 'S O'N H 8-2 The title compound was prepared according to the protocol described in Scheme 8, except tetrahydro-2H-pyran-4-amine was substituted for 3-morpholin-4-ylpropan-1-amine. 2 15 {[2-Methyl-6-(tetrahydro-2H-pyran-4-ylamino)pyrimidin-4-yl] amino }-1 ,3-thiazole-5 carbonitrile was obtained as the freebase, mp; >250 0 C. 1H-NMR (500 MHz, DMSO-d6): 11.50 (1H, s), 8.22 (1H, s), 7.33 (1H, d, J=7.8 Hz), 5.89 (1H, s), 3.85 (1H, d, J=8.3Hz), 3.39 (2H, t, J=11.3 Hz), 2.39(3H, s), 1.82 (2H, d, J=12.5 Hz), 1.42 (2H, br q). M+1 = 317.3 20 Synthesis of 2-[(6-{[3-(1H-imidazol-1-yl)propyl] amino}-2-methylpyrimidin-4-yl)amino]-1,3 thiazole-5-carbonitrile (8-3) N N H N N N S H 8-3 - 72 - WO 2004/041164 PCT/US2003/034100 3-(1H-imidazol-1-yl)propan-1-amine (49.7 mg, 0.40 mmol) was treated with excess N,N-diisopropyl-ethylamine (308 mg, 2.38 mmol) along with 2-[(6-chloro-2 methylpyrimidin-4-yl)amino]-1,3-thiazole-5-carbonitrile (6-1) (100 mg, 0.40 mmol) and n butanol (2.0 mL). The reaction was heated for 18 hours at 100'C. The reaction was cooled and 5 filtered. The solid was then washed with water, additional butanol, and ether. This gave 2-[(6 {[3-(1H-imidazol-1-yl)propyllamino}-2-methylpyrimidin-4-yl)amino]-1,3-thiazole-5-carbonitrile as a tan solid freebase. 1H-NMR (500 MHz, DMSO-d6): 11.09 (1H, br s), 8.22 (1H, s), 7.64 (1H, s), 7.40 (1H, t J=5.62Hz), 7.20 (1H, t J=1.22Hz), 6.89 (1H, s), 5.86 (1H, br s), 4.02 (211, t J=6.84Hz), 3.17 (2H, br s), 2.39 (3H, s), 1.95 (2H, m J=6.83Hz). M+1 = 341 10 Synthesis of 2-[(6-{ [(1,1 -dioxidotetrahydrothien-3-yl)methyl]amino } -2-methylpyrimidin-4 yl)aminol-1,3-thiazole-5-carbonitrile (8-4) 0 NH 0 .~ NC N N S H 8-4 Racemic 1-(1,1-dioxidotetrahydrothien-3-yl)methanamine (60 mg, 0.40 mmol) 15 was added to a flask along with excess N,N-diisopropylethylamine (308 mg, 2.38 mmol), 2-[(6 chloro-2-methylpyrimidin-4-yl)amino]-1,3-thiazole-5-carbonitrile (6-1) (100 mg, 0.40 mmol), and n-butanol (2.0 mL). The reaction was heated for 18 hours at 100"C. Additional amine and DIEA were added (1eq/3eq) and the reaction was allowed to continue to heat for an additional 18 hours. The solvents were removed and the residue purified on a prep HPLC. This gave 2-(6 20 { [(1,1-dioxidotetrahydrothien-3-yl)methyl]anino}-2-methylpyrimidin-4-yl)amino]-1,3-thiazole 5-carbonitrile as the trifluoroacetic acid salt. 1H-NMR (500 MHz, DMSO-d6): 11.98 (1H, br s), 8.23 (1H, s), 7.55 (1H, br s), 5.92 (1H, br s), 3.41 (2H, br s), 3.21 (2H, m), 3.21 (2H, m), 3.06 (1H, m), 2.85 (1H, m), 2.64 (1H, m), 2.41 (3H, s), 2.23 (1H, br s), 1.83 (1H, m). M+1 = 365 25 Synthesis of 2-({6- [(1,4-dioxan-ylmethyl)amino]-2-methylpyrimidin-4-yl I amino)-1,3-thiazole-5 carbonitrile (8-5) -73 - WO 2004/041164 PCT/US2003/034100 0 NH N N N N CN H 8-5 Racemic 1,4-dioxan-2-ylmethylamine (46 mg, 0.40 mmol) was added to a flask along with excess N,N-diisopropylethylamine (308 mg, 2.38 mmol), n-butanol (2.0 mL) and 2 [(6-chloro-2-methylpyrinmidin-4-yl)amifno]-1,3-thiazole-5-carbonitrile (6-1) (100 mg, 0.40 5 mmol). The reaction heated for 18 hours at 100'C. The solvents were removed and the residue purified on the prep HPLC. This yielded 2-({6-[(1,4-dioxan-2-ylmethyl)aminol-2 methylpyrimidin-4-yl}amino)-1,3-thiazole-5-carbonitile as the trifluoroacetic acid salt. 1H NMR (500 MHz, DMSO-d6): 12.01 (1H, br s), 8.23 (1H, s), 7.53 (1H, br s), 5.98 (111, br s), 3.74 (21, d J=11.72 Hz ), 3.64 (211, d J=11.23Hz), 3.57 (1H, t J=11.23), 3.46 (111, t J=11.23Hz), 10 3.32 (2H, br s), 3.25 (1H, t J=11.48Hz), 2.42 (3H, s). M+1 = 333 Synthesis of 2-({6-[(3-morpholin-4-ylpropyl)aminopyrimidin- 4 -yl}amino)-1,3-thiazole-5 carbonitrile (8-6) N NH N C N N CN H 8-6 15 3-Morpholin-4-ylpropan-1-amine (182 mg, 1.26 mmol) was added to a flask with excess N,N-diisopropylethylamine (327 mg, 2.53 mmol) along with 2-[(6-chloropyrimidin-4 yl)amino]-1,3-thiazole-5-carbonitrile (100 mg, 0.421 mmol), and n-butanol (2.0 mL). The reaction heated for 18 hours at 100 C. The reaction was cooled and diluted with ethyl acetate (50 mL). The ethyl acetate layer was washed with brine/water (50/10 mL). The organic layer 20 was dried with MgSO4 and the solvent removed to yield a gum. The gum was placed on the prep HPLC and purified in the usual way. This gave 2-({6-[(3-morpholin-4 ylpropyl)amino]pyrimidin-4-yl } amino)-1,3-thiazole-5-carbonitrile as the trifluoroacetic salt. 1 H-NMR (500 MHz, DMSO-d6): 12.05 (1H, br s), 9.78 (1H, br s), 8.25 (1H, s), 7.61 (11H, br s), -74 - WO 2004/041164 PCT/US2003/034100 6.08 (1H, br s), 3.97 (2H, br s), 3.64 (2H, br s), 3.42 (2H, br s), 3.38 (211, br s), 3.15 (2H, br s), 3.07 (2H, br s), 1.90 (2H, br s). M+1 = 346 Synthesis of 2-({6-[(1,1-Dioxidotetrahydrothien-3-yl)aminol-2-methylpyrimidin-4-yl} amino) 5 1,3-thiazole-5-carbonitrile (8-7) S NH NCN N N S C H 8-7 2-[(6-Methoxy-2-methylpyrimidin-4-yl)amino]-1,3-thiazole-5-carbonitrile (100.0 mg, 0.397 mmol) was added to a reaction flask along with 1,1-dioxidotetrahydrothien-3-ylamine (107.3 mg, 0.79 mmol), N-ethyl-N,N-diisopropylamine (410 pL, 2.38 mmol) and n-butanol (3.0 10 mL). The reaction was heated at reflux for one week. The reaction was cooled and the n-butanol was removed. The residue was treated with trifluoroacetic acid (0.5 mL) and diluted with methanol (3.0 mL). The mixture was then purified on a preparative high pressure chromatograph. This yielded 2-({6-[(1,1-dioxidotetrahydro-thien-3-yl)aminol-2 methylpyrimidin-4-yl}aImino)-1,3-thiazole-5-carbonitrile as the trifluoroacetic acid salt. 1H 15 NMR (500 MHz, DMSO-d 6 ): 12.03 (1H, br s), 8.24 (1H, br s) 7.79 (1H, br s), 5.97 (1H, br s), 4.62 (1H, br s), 3.53 (111, br s ), 3.34 (1H, br s), 3.21 (1H, br s), 2.19 (1H, br s), 2.51 (1H, br s), 2.43 (3H, s), 2.12 (1H, br s). M+1 = 351 2-{[2-Methyl-6-(tetrahydrofuran-3-ylamino)pyrimidin-4-yl]amino}-1,3-thiazole-5-carbonitrile 20 (8-8) -75- WO 2004/041164 PCT/US2003/034100 0 NH N N N N s C H 8-8 2-[(6-Methoxy-2-methylpyrimidin-4-yl)amino]-1,3-thiazole-5-carbonitrile (100.0 mg, 0.397 mmol) was added to a reaction flask along with tetrahydrofuran-3-amine hydrochloride salt (136.3 mg, 0.79 mmol), N-ethyl-N,N-diisopropylamine (410 VL, 2.38 mmol) 5 and n-butanol (3.0 mL). The reaction was heated at reflux for one week. The reaction was cooled and the n-butanol was removed. The residue was treated with trifluoroacetic acid (0.5 mL) and diluted with methanol (3.0 mL). The mixture was then purified on a preparative high pressure chromatograph. This yielded 2-{[2-methyl-6-(tetrahydro-furan-3-ylamino)pyrimidin-4 yl]amino}-1,3-thiazole-5-carbonitrile as the trifluoroacetic acid salt. 1H-NMR (500 MHz, 10 DMSO-d6): 11.99 (1H, br s), 8.23 (1H, s) 7.65 (1H, br s), 5.93 (1H, br s), 4.98 (1H, br s), 3.83 (2H, m) 3.71 (1H, m), 3.52 (1H, br s), 2.42 (3H, s), 2.16 (1H, m), 1.80 (1H, br s). M+1 = 303 - 76 - WO 2004/041164 PCT/US2003/034100 SCHEME 9 H N N Br N 0 0 R H O DIEA H N N TEA HH C1 N N N -rNa 6-1H o DIEA

NH

2 n-BuOH H N Na 0 N H 9-1 N N NN S OCN H Synthesis of tert-butyl-1-[2-(isopropylamino)-2-oxoethyllpiperidin-4-vlcarbamate tert-Butyl piperidin-4-ylcarbamate (100.0 mg, 0.499 mmol) along with N,N 5 diisopropylethylamine (64.5 mg, 0.50 mmol), and methylene chloride (3.0 mL) were added to a reaction flask. 1-Bromo-N-isopropylacetamide (89.8 mg, 0.50 mmol) was slowly added to the flask and the reaction allowed to stir for an hour. The solvents were removed under high vac and the tert-butyl-1-[3-(isopropylamino)-2-oxopropyl]piperidin-4-ylcarbamate used as is in the next step. 10 - 77 - WO 2004/041164 PCT/US2003/034100 Synthesis of 2-(4-aminopiperidin-1-vl)-N-isopropylacetamide The tert-butyl 1-[2-(isopropylamino)-2-oxoethyl]piperidin-4-ylcarbamate was treated neat with trifluoroacetic acid and warmed at 60'C for approximately 15 minutes. The reaction was then cooled to room temperature and all of the excess TEA removed under high 5 vacuum. The 1-(4-aminopiperidinl-yl)-3-(isopropylamino)acetone obtained was used as is in the next step. Synthesis of 2-[-({6-[(5-cyano-1,3-triazol-2-ylamino]-2-methylpyrimidin-4-ylI amino)piperidin 1-yll-N-isopropylacetamide (9-1) 10 2-(4-Aminopiperidin-1-yl)-N-isopropylacetamide (95.0 mg, 0.50mmol) was treated with excess N,N-diisopropylethylamine (308 mg, 2.38 mmol) along with 2-[(6-chloro-2 methylpyrimidin-4-yl)aiino]-1,3-thiazole-5-carbonitrile (6-1) (100.0 mg, 0.397 mmol) and n butanol (2.0 mL). The reaction was heated at 100'C for three days. The solvent was removed and the crude material purified on the prep HPLC. This yielded 2-[-({6-[(5-cyano-1,3-triazol-2 15 ylamino]-2-methylpyrimidin-4-yl } amino) piperidin-1-yl]-N-isopropylacetamide as the trifluoroacetic acid salt. 1H-NMR (500 MHz, CD30D): 8.02 (1H, s), 6.01 (111, s), 4.03 (2H, m) 3.90 (2H, br s), 3.70 (2H, br s), 2.56 (3H, s), 2.27 (2H, br s), 1.91 (2H, br s), 1.18(6H, d). M+1 =415 20 SCHEME 10 0 N C N N

NH

2 N N S CN DIEA H o-xylene 6-1 0 O NH O Na NH TFA HN NH 'NH N N ~ N NN CN N CN N N s NN 10-1 H 10-2 H - 78- WO 2004/041164 PCT/US2003/034100 Synthesis of tert-butyl-4-({6-[(5-cyano-1,3-thiazol-2-ylamino]-2-methylpyrimidin-4 yl lamino)piperidine-1-carboxylate (10-1) 2-[(6-Chloro-2-methylpyrimidin-4-yl)aminol-1,3-thiazole-5-carbonitrile (6-1) (125.0 mg, 0.50 mmol) was added to a solution of tert-butyl-4-aminopiperdine-1-carboxylate 5 (201 mg, 1.0 mmol) and N,N-diisopropylethylamine (0.27 mL, 1.5 mrnol) in o-xylene (3.0 mL). The reaction mixture was heated at reflux for three days. The reaction was cooled and excess solvents removed. The residue was purified by chromatography on silica gel eluting with a 40 80% ethyl acetate/hexane gradient The appropriate fractions were combined and the solvents evaporated to give the desired product as a crystalline freebase, mp: >250 0 C. 10 lH-NMR (500 MHz, CDCl3): 9.15 (1H, br s), 7.91 (1H, s), 5.56 (1H, s), 4.88 (1H, br s) 4.06 (2H, br s), 3.72 (111, m), 2.99 (2H, t J=10.5 Hz), 2.52 (3H, s),2.01 (2H, br d, J=12.5Hz), 1.48 (9H, s), 1.42 (2H, m). M+1 = 416.3 Synthesis of 2-{[2-methyl-6-(piperidin-4-ylaiino)pyrimidin-4-yl]amino}-1,3-thiazole-5 15 carbonitrile (10-2) A solution of tert-butyl-4-({6-[(5-cyano-1,3-thiazol-2-yl)anino] 2-methylpyrimidin-4-yl}amino)piperidine-1-carboxylate (60 mg, 0.144 mmol) in chloroform (5 mL) containing TFA (1.0 mL) was stirred at room temperature over night. The solvents were evaporated and the residue was chased with chloroform (2X). The residue was dissolved in a 20 minimum amount of methanol and diluted with diethyl ether and the title compound crystallized out as the TFA salt, mp: >250 0 C. 1 H-NMR (500 MHz, DMSO-d6): 12.0 (1H, s), 8.52(1H, br s), 8.32(1H, br s), 8.24 (1H,s), 7.47 (1H, br d, J=6.3H1z), 5.93(1H,s), 3.28(2H, br d J=11.6Hz), 3.05 (2H,m), 2.41(3H, s), 2.02(2H,br d, J=13.5Hz), 1.60 (2H,m). M+1 = 316.3 - 79 - WO 2004/041164 PCT/US2003/034100 SCHEME 11 CI 0 NON N N s CN

NH

2 H DIEA 6-1 dioxane NH TFA NH 0N N 0 N N N~N N N s CN N N CN 11-1 H 11-2 H Synthesis of tert-butyl-4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]methyl}-2-methylpyrimidin-4 yi amino)piperidine-1-carboxylate (11-1) 5 2-[(6-Chloro-2-methylpyrimidin-4-yl)aminol-1,3-thiazole-5-carbonitrile (6-1) (125.0 mg, 0.50 mmol) was added to a solution of tert-butyl-4-aminomethylpiperdine-1 carboxylate (142 mg, 0.66 mmol) and N,N-diisopropyl-ethylamine (0.25 mL, 1.44 mmol) in dioxane (1.5 mL). The reaction mixture was heated at reflux for three days. The reaction was cooled and ethyl acetate (4 mL) was added. Product slowly precipitated and was collected by 10 filtration. The mother liquors were purified further by chromatography on silica gel eluting with ethyl acetate. The appropriate fractions were combined and the solvents evaporated to give the desired product as a crystalline freebase, mp: >250cC. 1H-NMR (500 MHz, CDCl3):11.15 (1H, s), 7.89 (1H, s), 5.76 (1H, s),5.27 (1H, br s), 4.12 (2H, br s), 3.16 (211, br s), 2.69 (2H, m), 2.48 (3H, s), 1.73(2H, br d, J=9.5Hz), 1.45 (9H, s), 1.21 (2H, m). M+1 = 430.4 15 Synthesis of 2-({2-methyl-6-[(piperidin-4-ylmethyl)amino]pyrimidin-4-yl} amino)-1,3-thiazole 5-carbonitrile (11-2) A solution of tert-butyl-4-({6-[(5-cyano-1,3-thiazol-2-yl)amino] methyl}-2 methylpyrimidin-4-yl}amino)piperidine-1-carboxylate (112 mg, 0.26 mmol) in chloroform (5 20 mL) containing TFA (1.0 mL) was stirred at room temperature over night. The solvents were evaporated and the residue was dissolved in a minimum amount of methanol and diluted with - 80 - WO 2004/041164 PCT/US2003/034100 diethyl ether and the title compound crystallized out as the TFA salt, mp: >250'C. 1H-NMR (500 MHz, DMSO-d6):11.9(1H, s), 8.58 (1H, br s), 8.23(1H,s), 7.89(1H, br s),7.52(1H, br s), 5.92 (1H,br s), 3.27(2H, br d J=10.7Hz), 2.86 (2H,br q), 2.36(3H, s), 1.81(2Hbr d, J=13.4Hz), 1.31 (2H,br q). M+1 = 330.3 5 SCHEME 12 0 O N C1 ~ 00 CI O N O N 4 IOH N~ N N ~ DIEA4 N N I CN dioxane N N CN H 12-1 H HN Ha 0 TFA N N N N S CN 12-2 H Synthesis of tert-butyl-4-({6-[(5-cyano-1,3-triazol-2-ylaminol-5-methylpyrimidin-4 Vlloxy)piperidine-1-carboxylate (12-1) 10 Sodium hydride (60% in mineral oil, 80mg 2.0 mmol) was added to a solution of tert-butyl-4-hydroxypiperdine-1-carboxylate (151 mg, 0.75 mmol) in toluene (2.0 mL). After gas evolution ceased 2-[(6-chloro-5-methylpyrimidin-4-yl) amino]-1,3-thiazole-5-carbonitrile (126.0 mg, 0.50 mmol) was added to the reaction. Anhydrous dioxane (1 mL) and dry DMSO (0.5 mL) were added to dissolve the resultant suspension and the reaction mixture was heated at reflux for 15 six hours. The reaction was cooled and ethyl acetate was added. The organic layer was washed with NaHCO3, dried, filtered, and the solvents evaporated. The residue was purified by chromatography on silica gel eluting with a 40% ethyl acetate/hexane. The appropriate fractions were combined and the solvents evaporated to give the desired product as a white solid. 1H - 81 - WO 2004/041164 PCT/US2003/034100 NMR (500 MHz, CDC13): 8.48(lH, s), 8.46 (111, br s), 7.94 (111, s), 5.36 (1H, m), 3.74 (2H, m), 3.36 (2H, m), 2.15 (3H, s), 2.00 (2H, m), 1.77 (211, m), 1.48 (9H, s). M+1 = 417.3 Synthesis of 2-{[5-methyl-6-(piperidin-4-ylamino)pyrimidin-4-yl]oxy}-1,3-thiazole-5 carbonitrile (12-2) 5 A solution of tert-butyl-4-({6-[(5-cyano-1,3-thiazol-2-yl)amino]-5 methylpyrimidin-4-yl}oxy)piperidine-1-carboxylate (64 mg, 0.15 mmol) in chloro-form (6 mL) containing TFA (2.0 mL) was stirred at room temperature over night. The solvents were evaporated and the residue was chased with chloroform (2X). The residue was dissolved in a minimum amount of methanol and diluted with diethyl ether and the title compound crystallized 10 out as the TFA salt, mp: 214-216 0 C. 1H-NMR (500 MHz, DMSO-d6):11.8 (111, br s), 8.56(1H, s), 8.52(1H,br s), 8.36 (111, s), 5.36(1H,m), 3.25(2H, m), 3.18 (2H,m), 2.19(3H, s), 2.12 (211,m), 1.91 (2H,m). M+1 = 317.3 SCHEME 13 HO HS N 4A0 N ON - C Pyridine N NH 2 N NH 2 1NNaOH 13-1 00 N N-N N 1 3 -2 NC I N a H N C NaH I CN 15 N NH 2 Dioxane H 13-3 Synthesis of 6-amino-2-methylpyrimidine-4-thiol (13-1) The title compound was prepared by modification of the procedure described Chem. Pharm. Bull (Tokyo), 11, 912-917 (1963) [Chem. Abstr. 60:1748a]. Phosphorus 20 pentasulfide (12.2 gm, 50 mmol) was added to pyridine (80 mL) and stirred for 30 minutes. Then 6-chloro-2-methylpyrimidin-4-amine (2.5 gm, 20 mmol) was added and the mixture was heated at 1 10'C for 24 hours. The pyridine was removed under reduced pressure and the yellow solid suspended in water (50 mL). 5 N NaOH was added until nearly all solid dissolved in the -82- WO 2004/041164 PCT/US2003/034100 solution. This solution was filtered and acetic acid was added as the yellow product precipitated. After cooling this mixture in an ice bath the product was collected by filtration, rinsed with water and air-dried to give 6-amino-2-methylpyrimidine-4-thiol. 1 H-NMR (500 MHz, DMSO-d6): 12.2 (1H, s), 7.02 (2H, br s), 6.00(1H, s), 2.23(3H, s). 5 Synthesis of 2-methyl-6-[(2-morpholin-4-ylethL)thiolpyrinidin-4-amine (13-2) Dissolved 6-amino-2-methylpyrimidine-4-thiol (102 mg, 0.72 mmol) in 1 N NaOH (2.5 mL) and added N-(2-chloroethyl)morpholine hydrochloride (140 mg, 0.75 mmol) to the solution. After stirring at ambient temperature for 18 hours the pH was adjusted to -4 by 10 addition of acetic acid and the product was isolated by extraction into ethyl acetate. This organic layer was dried over anhydrous sodium sulfate, the solution filtered, and the solvent removed. The residue was triturated with diethyl ether to the title compound as a yellow solid. 1H-NNMR (500 MHz, CDCl3): 6.14 (1H, s), 4.67 (2H, br s), 3.74 (4H,t, J=4.6 Hz), 3.27 (2H,t, J=7.5 Hz), 2.67 (2H, t, J=7.6Hz), 2.54 (4H, m), 2.45 (3H, s). M+1 = 255.4 15 Synthesis of 2-({2-methyl-6-[(2-morpholin-4-ylethyl)thio]pyrimidin-4-y I amino)- 1,3-thiazole-5 carbonitrile (13-3) 0 ON S NN N N N N S H 13-3 Sodium hydride (60% in mineral oil, 65 mg, 1.6 mmol) was added to a partial 20 suspension of 2-methyl-6--[(2-morpholin-4-ylethyl)thio]pyrimidin-4-amine (100 mg, 0.39 mmol) and stirred for 15 minutes. 2-Chloro-1,3-thiazole-5-carbonitrile (58 mg, 0.40 mmol) was then added and the mixture was heated at 60*C and the progress of the reaction was monitored by LC/MS. Additional sodium hydride (43 mg) and 2-chloro-1,3-thiazole-5-carbonitrile (47 mg) was added to drive reaction to near completion. The reaction mixture was cooled, diluted with 25 ethyl acetate, acidified with some acetic acid and then washed with aq. satd. sodium bicarbonate. The dried organic solution was filtered and the solvent removed. The residue was subjected to column chromatography on silica gel and eluted with a 1-3% methanol/ ethyl acetate (saturated NH40H) gradient. The appropriate fractions were combined and the solvent evaporated. The -83- WO 2004/041164 PCT/US2003/034100 residue was triturated with diethyl ether/ethyl acetate to give the title compound as a freebase, mp: 197-198*C. 1H-NMR (500 MHz, DMSO-d6): 12.2(1H,br s), 8.33 (1H,s), 6.76 (1H, s), 3.57 (4H,m), 3.28 (2H, br t, J=7.2 Hz), 2.61 (2H, m), 2.57 (3H, s), 1.98 (2H,m), 2.46 (4H,m). M=1 = 363.3 5 SCHEME 14 N SH N S 1) 1 N NaOH N 2) ZnC 2 N

NH

2 NH 2 2 O N Cl CN OH S NaH DEAD 14-1 N Dioxane Ph 3 P THF N NH 2 0 TFA HN S CH 2 Cl 2 14-2 N N \ CN N' N CN N NN N S H 14-3 Synthesis of tert-butyl 4-[( 6 -amino-2-methylpyrimidin-4-yflthiolpiperidine-1-carboxylate (14-1) Potassium hydroxide (86%, 260 mg, 4 mmol) was dissolved in a mixture of 10 ethanol (15 mL) and methanol (5 mL) and then 6-amino-2-methylpyrimidine-4-thiol (564 mg, 4 mmol) was added. This mixture was stirred and slightly warmed until most of solid had dissolved. A solution of 1 M zinc chloride in ether (2 mL) was added dropwise as a precipitate formed instantly. After stirring for one half hour, the solid was isolated by filtration and rinsed - 84- WO 2004/041164 PCT/US2003/034100 with ethanol, methanol and diethyl ether and air-dried to give bis-(6-amino-2-methylpyrimidine 4-thio) zinc. Tert-butyl 4-hydroxypiperdine-1-carboxylate (285 mg, 1.4 mmol) was dissolved in dry tetra-hydrofuran (5 mL) and then bis-(6-amino-2-methylpyrimidine-4-thio) zinc (350 mg, 5 1 mmol) and triphenyl phosphine (739 mg, 2.8 mmol) were added. This mixture was kept in the dark (covered with aluminum foil) and cooled in an ice bath and diethyl diazodicarboxylate (DEAD) (0.44 mL, -2.8 mmol) was added dropwise via syringe. The reaction was stirred for -18 hours. The solvent was removed under vacuum and the residue was subjected to chromatography on silica gel eluting with a 20-80% ethyl acetate/hexane gradient. Fractions containing product 10 and triphenyl phosphine oxide were combined and the solvent removed. This mixture was rechromatographed on silica gel and eluted with 0-3% methanol/ethyl acetate. The appropriate fractions were combined and the solvent removed to give the title compound as a viscous semisolid. 1H-NMR (500 MHz, CDCl3): 6.12 (1H, s), 3.99 (H, m), 3.92 (211, br s), 3.09 (2H,t, J=11 Hz), 2.45 (3H, s), 2.04 (2H, br d, J=13 Hz), 1.62 (2H, m), 1.46 (911, s). M+1 = 325.4 15 Synthesis of tert-butyl 4-({ 6-[(5-cyano-1,3-thiazol-2-yl)amino]pyrimidin-4-yl }thio) piperidine-1 carboxylate (14-2) Sodium hydride (60% in mineral oil, 22 mg, -0.5 mmol) was added to a solution of tert-butyl 4-[(6-aminopyrimidin-4-yl)thio]piperidine-1-carboxylate (37 mg, 0.114 mmol) in 20 dry dioxane (2 mL). After gas evolution ceased (-1/4 hour) 2-chloro-1,3-thiazole-5-carbonitrile (19 mg, 0.13 mmol) was added and the reaction was warmed to -60*C for 22 hours. By LC/MS the reaction was incomplete so additional sodium hydride (19 mg, 0.5 mmol) and 2-chloro-1,3 thiazole-5-carbonitrile (19 mg, 0.13 mmol) were added and the reaction mixture was heated for another 20 hours. The cooled reaction was diluted with ethyl acetate and acetic acid (0.09 mL, 25 1.5 mmol) was added. This solution was washed with NaHCO3 solution. The ethyl acetate extract was dried over anhydrous Na2SO4, filtered and evaporated. The residue was chromato graphed on silica gel eluting with a 30-80% ethyl acetate/hexane gradient. The appropriate fractions were combined and the solvents removed to give the title compound as a white solid. 1H-NMR (500 MIHz, CDCl3): 10.5 (111, br s), 7.96 (1H,s), 6.49 (111, s), 4.14 (1H,m), 3.94 (2H, 30 br s), 3.13(2H, m), 2.67 (311, s), 2.07 (2H,br d, J=7.8 Hz), 1.66 (2H,m), 1.48 (9H, s). M+1 = 433.4 - 85 - WO 2004/041164 PCT/US2003/034100 Synthesis of 2-1[6-(piperidin-4-vlthio)pyrimidin-4-ll amino }-1,3-thiazole-5-carbonitrile (14-3) H N N N H 14-3 A solution of tert-butyl 4-({6-[(5-cyano-1,3-thiazol-2-yl)aminol pyrimidin-4 yl}thio)piperidine-1-carboxylate (10 mg, 0.023 mmol) in methylene chloride (4 mL) containing 5 TFA (0.5 mL) was stirred at room temperature over night. The solvents were evaporated and the residue was chased with chloroform (2X). This residue was triturated with diethyl ether to give the title compound as a TFA salt, mp: 243-244"C. 1H-NMR (500 MHz, DMSO-d6): 12.2 (111, br s), 8.58(1H, br s), 8.38 (1H, br s), 8.35 (1H,s), 6.77 (1H,s), 4.06 (1H,m), 3.29(2H,m), 3.11 (2H,m), 2.60 (3H, s), 2.11 (2H,br d, J=14 Hz), 1.78 (2H,br q, J=10.6 Hz). M+1 = 333.3 10 - 86 -

Claims (57)

1. A compound of Formula I R 5 X (CH 2 M ( H 2 )R N N N N S R 1 3 R .5 or a pharmaceutically acceptable salt or stereoisomer thereof, wherein Xis 0, S or NR 3 ; 10 mis 0, 1, 2 or 3; n is 0, 1, 2 or 3; RI is: 15 1) H, 2) Or(C1-C6)perfluoroalkyl, 3) OH, 4) CN, 5) halogen, 20 6) (C=O)rOs(C-C1O)alkyl, 7) (C=0)rOs(C2-C1o)alkenyl, 8) (C=O)rOs(C2-C10)alkynyl, 9) (C=O)rOsaryl, 10) (C=0)rOsheterocyclyl, or 25 11) (CO-C6)alkyl-NRaRb, wherein r and s are independently 0 or 1, and said alkyl, alkenyl, alkynyl, aryl and heterocyclyl is optionally substituted with one or more substituents selected from R 6 ; R 2 is: - 87 - WO 2004/041164 PCT/US2003/034100 1) H, 2) Or(C1-C6)perfluoroalkyl, 3) OH, 4) CN, 5 5) halogen, 6) (C=O)rOs(C1-C10)alkyl, 7) (C=O)rOs(C2-CIO)alkenyl, 8) (C=O)rOs(C2-C1O)alkynYl, 9) (C=O)rOsaryl,. 10 10) (C=O)rOsheterocyclyl, or 11) (CO-C6)alkyl-NRaRb, wherein r and s are independently 0 or 1, and said alkyl, alkenyl, alkynyl, aryl and heterocyclyl is optionally substituted with one or more substituents selected from R6; 15 R 3 is: 1) H, 2) SO 2 Rc, 3) (C=O)rRC, wherein r is 0 or 1, or 4) CO2Rc; 20 R 4 is: 1) H, 2) Or(C1-C6)perfluoroalkyl, 3) OH, 25 4) CN, 5) halogen, 6) (C=0)rOs(Ci-C1O)alkyl, 7) (C=O)rOs(C2-C1o)alkenyl, 8) (C=O)rOs(C2-C10)alkynyl, 30 9) (C=O)rOsaryl, 10) (C=O)rOsheterocyclyl, or 11) (CO-C6)alkyl-NRaRb, wherein r and s are independently 0 or 1, and said alky, alkenyl, alkynyl, aryl and heterocyclyl is optionally substituted with one or more substituents selected from R 6 ; 35 - 88 - WO 2004/041164 PCT/US2003/034100 R 5 is heterocyclyl wherein said heterocyclyl contains one or two additional heteroatoms selected from N, 0 and S, and is optionally substituted with one or more substituents selected from R 6 ; R 6 is: 5 1) Or(C=O)sNRaRb, 2) (C=O)rOsaryl, 3) (C=O)rOs-heterocyclyl, 4) halogen, 5) OH, 10 6) oxo, 7) O(Ci-C3)perfluoroalkyl, 8) (Ci-C3)perfluoroalkyl, 9) (C=0)rOs(C-C1O)alkyl, 10) CHO, 15 11) CO2H, or 12) CN, wherein r and s are independently 0 or 1, and said alkyl, aryl, and heterocyclyl are optionally substituted with one or more substituents selected from Rd; 20 Ra and Rb are independently: 1) H, 2) (C=0)r(C1-C10)alkyl, 3) S(O)2Rc, 4) (C=0)rheterocyclyl, 25 5) (C=O)raryl, or 6) CO2Rc, wherein r is 0 or 1 and said alkyl, heterocyclyl, and aryl optionally substituted with one or more substituents selected from Rd, or 30 Ra and Rb are taken together with the nitrogen to which they are attached to form a monocyclic or bicyclic heterocycle with 5-7 members in each ring and optionally containing, in addition to the nitrogen, one or two additional heteroatoms selected from N, 0 and S, said monocyclic or bicyclic heterocycle optionally substituted with one or more substituents selected from Rd; 35 Re is (C1-C6)alkyl, aryl, benzyl, or heterocyclyl; - 89 - WO 2004/041164 PCT/US2003/034100 Rd is: 1) (C=O)rOs(C1-C1O)alkyl, wherein r and s are independently 0 or 1, optionally substituted with up to three substituents selected from OH, (C1 C6)alkoxy, halogen, CN, oxo, N(Re) 2 and S(O) 2 Rc, 5 2) (C=O)N(Re) 2 , 3) Or(C1-C3)perfluoroalkyl, 4) (CO-C6)alkylene-S(O)mRc, wherein m is 0, 1 or 2, 5) oxo, 6) OH, 10 7) halogen, 8) CN, 9) (CO-C6)alkylene-aryl, optionally substituted with up to three substituents selected from Re, 10) (CO-C6)alkylene-heterocyclyl, optionally substituted with up to three 15 substituents selected from Re, 11) (CO-C6)alkylene-N(Re) 2 , 12) C(O)Rc, 13) CO2Rc, 14) C(O)H, or 20 15) CO2H; and Re is H, (C1-C6)alkyl, aryl, heterocyclyl or S(O) 2 Rc.

2. The compound of Claim 1 or a pharmaceutically acceptable salt or 25 stereoisomer thereof, wherein R1 is selected from: 1) H, 2) CN, 3) halogen, 4) OH, 30 5) (C=O)rOs(C1-C1o)alkyl, and 6) (C=0)rOs(Ci-C1o)alkyl-NRaRb.

3. The compound of Claim 2 or a pharmaceutically acceptable salt or stereoisomer thereof, wherein R 2 is selected from: 35 1) H, -90- WO 2004/041164 PCT/US2003/034100 2) CN, 3) OH 4) halogen, 5) phenyl, wherein said phenyl is optionally substituted with one or more 5 substituents selected from R 6 , 6) (C=0)rOs(C-C1O)alkyl, and 7) (C=O)rOs(C1-C1O)alkyl-NRaRb.

4. The compound of Claim 3 or a pharmaceutically acceptable salt or 10 stereoisomer thereof, wherein R 4 is selected from: 1) H, 2) CN, 3) halogen, 4) (C1-C6)alkyl, 15 5) (Cl-C6)perfluoroalkyl, and 6) (C=0)rOsheterocyclyl.

5. The compound of Claim 4 or a pharmaceutically acceptable salt or stereoisomer thereof, wherein RI is H; R 2 is CN or phenyl; R 3 is H; and R 4 is H or (CI 20 C6)alkyl.

6. A compound of Claim 1 selected from: tert-butyl-4-({ 6-[(5-cyano-1,3-thiazol-2-yl)aminolpyrimidin-4-yl}oxy)piperidine-1-carboxylate; 2-{ [6-(piperidin-4-yloxy)pyrimidin-4-yl]amino}-1,3-thiazole-5-carbonitrile; 25 tert-butyl-4-({6-[5-phenyl-1, 3 -thiazol-2-yl)amino]pyrimidin-4-yl}oxy)piperidine-1-carboxylate; N-(5-phenyl-1, 3 -thiazol-2-yl)-6-(piperidin-4-yloxy)pyrimidin-4-amine; tert-butyl-4-[({6-[5-cyano-1,3-thiazol-2-yl)amino]pyrimidin-4-yl}oxy)methyl]-piperidine-1 carboxylate; tert-butyl-4-[({6-[(5-phenyl-1,3-thiazol-2-yl)amino]pyrimidin-4-yl}oxy)methyl]-piperidine-1 30 carboxylate; N-(5-phenyl-1,3-thiazol-2-yl)-6-(piperidin-4-ylmethoxy)pyrimidin-4-amine; 2-{ [ 2 -methyl-6-(piperidin-4-yloxy)pyrimidin-4-yl]amino }-1,3-thiazole-5-carbonitrile; N-(5-phenyl-1, 3 -thiazol- 2 -yl)- 6 -(piperidin-4-yloxy)-2-methylpyrimidin-4-amine; 2-({ 2 -methyl-6-[(3R)-pyrrolidin-3-yloxy]pyrimidin-4-yl }amino)-1,3-thiazole-5-carbonitrile; 35 2-({ 2 -methyl- 6 -[(3S)-pyrrolidin-3-yloxy]pyrimidin-4-yl }amino)-1,3-thiazole-5-carbonitrile; -91- WO 2004/041164 PCT/US20031034100 2-12-methyl-6- { I-( 2 -morpholin-4-ylethyl)piperidin-4-y1]oxylpyriidin-4-y1)arnino] 1,3 thiazole-5-carbonitrile; 2- [4-({ 6- [5-cyano-1 ,3-thiazol-2-yl)aminol-2-methylpyrimidin-4-yl }oxy)piperidin- 1-yl]-N isopropylacetamide; 5 2- {[ 2 -methyl-6-(3-morpholin-4-ylpropoxy)pyrimidin-4-yl] amino) - -1,3-thiazole-5-carbonitrile; 2-{ 12-methyl-6-(2-morpholin-4-ylethoxy)pyrimidin-4-ylI amino I -1,3-thiazole-5-carbonitrile; 2- {[2-methyl-6-(2-piperidin- 1-ylethoxy)pyrimidin-4-yl]amino } -1 ,3-thiazole-5-carbonitrile; 2-({ 2 -methyl-6-[(2-morpholin-4-ylethyl)anino]pyrimidin-4-yl I amino)- 1 ,3-thiazole-5 carbonitrile; 10 2- {16-(piperidin-4-ylmethoxy)pyrimidin-4-yl amino 1-1 ,3-thiazole-5-carbonitrile; 2- { [2-methyl-6-(piperidin-4-ylmethoxy)pyrin-idin-4-yl] amino 1- 1,3 -thiazole-5-carbonitrile; 2-({1 6 -[(3-morpholin-4-ylpropyl)amino]pyrimidin-4-yl I amino)- 1,3-thiazole-5-carbonitrile; 2-{ [ 2 -methyl-6-(tetrahydro-2H-pyran-4-ylamino)pyrimidin-4-yl] amino} 1 ,3-thiazole-5 carbonitrile; 15 2-[(6-1{[ 3 -(lfl-imidazol-1I-yl)propyll amino }-2-methylpyrimidin-4-yl)amino] -1,3-thiazole-5 carbonitrile; 2- [(6-1{ [(1, 1-dioxidotetrahyrothien-3-yl)methyl] amino 1-2-methylpyrimidin-4-yl) amino]-1 ,3 thiazole-5-carbonitrile; 2-(f 6-[(1 , 4 -dioxan-2-ylmethyl)amino]-2-methylpyrimidin-4-yl I amino)- 1 ,3-thiazole-5 20 carbonitrile; 2-({ 6-[(3-morpholin-4-ylpropyl)amino]pyrin-idin-4-yl }amnino)-l1,3-thiazole-5-carbonitrile; 2-[-({ 6-115-c yanio- 1,3-thiazol-2-ylamino]-2-methylpyrimidin-4-yl Iamino)piperidin- 1-yl]-N isopropylacetam-ide; tert-butyl-4-({ 6-[(5-cyano- 1 ,3-thiazol-2-ylaminol-2-methylpyrimidin-4-yl I amino) piperidine- 1 25 carboxylate; 2-{ [2-methyl-6-(piperTidin-4-ylamino)pyrimidin-4-ylI amino I1- 1,3-thiazole-5-carbonitrile; tert-butyl-4-({ 6-[(5-cyano- 1,3-thiazol-2-yl)aminojmethyl } -2-methylpyrTimidin-4-yl I amino) piperidine-l1-carboxylate; 2-({ 2-methyl-6- [(piperidin-4-ylmethyl)amino]pyrimidin-4-y I}amino)- 1,3-thiazole-5-carbonitrile; 30 2-{ [5-methyl-6-(piperidin-4-ylamino)pyrimidin-4-ylloxy} -1 ,3-thiazole-5-carbonitrile; tert-butyl-2-[({ 6-1(5-cyano- 1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-yl }oxy) methyl] morpholine-4-carboxylate; 2-{ [2-methyl-6-(morpholin-2-ylmethoxy)pyrimidin-4y1] amino) 1-1,3 -thiazole-5-carbonitrile; 2-{ [ 2 -methyl-6-(tetrahydro-2-pyran-4-yloxy)pyrimicin-4-y1]amino }-1 ,3-thiazole-5-carbonitrile; 35 2-{ [2-isopropyl-6-(piperidin-4-yloxy)pyrimidin-4-yl] amino I-i ,3-thiazole-5-carbonitrile; - 92 - WO 2004/041164 PCT/US2003/034100 2-({6-[(1, 1-dioxidotetrahydrothien-3-yl)amino] -2-methylpyrimidin-4-yl } amino)- 1,3-thiazole-5 carbonitrile; 2-{ [ 2 -methyl- 6 -(tetrahydrofuran-3-ylamino)pyrinidin-4-yl]amino }-1,3-thiazole-5-carbonitrile; tert-butyl{ 4-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-y } oxy) 5 methyl]piperidin-1-yl }acetate; {4-[({6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4-yl oxy)methyl] piperidin-1 yl}acetic acid; N-(tert-butyl)-2-{4-[({6-[(5-cyano-1, 3 -thiazol-2-yl)amino]-2-methylpyrimidin-4 yl}oxy)methyl]piperidin-1-yl acetamide; 10 2-({ 2-methyl-6-[(2-morpholin-4-ylethyl)thio]pyrimidin-4-y } amino)- 1,3-thiazole-5-carbonitrile; and 2-{ [ 6 -(piperidin-4-ylthio)pyrimidin-4-yl]amino }-1,3-thiazole-5-carbonitrile; or a pharmaceutically acceptable salt or stereoisomer thereof. 15

7. A compound which is 2-({ 2 -methyl-6-[(3S)-pyrrolidin-3-yloxypyrimidin 4-yl }amino)-1,3-thiazole-5-carbonitrile H N NN N NN S CN H or a pharmaceutically acceptable salt or stereoisomer thereof. 20

8. A compound which is: N-(5-phenyl-1, 3 -thiazol-2-yl)-6-(piperidin-4-yloxy)pyrimidin-4-amine HN N N N S H or a pharmaceutically acceptable salt thereof. - 93 - WO 2004/041164 PCT/US2003/034100

9. A compound which is: 2-{[ 2 -methyl-6-(piperidin-4-yloxy)pyrimidin-4-yl] amino }-1,3-thiazole-5-carbonitrile HN N N N S O 5 H or a pharmaceutically acceptable salt thereof.

10. A compound which is: 2-{ [ 2 -methyl- 6 -(morpholin-2-ylmethoxy)pyrimidin-4-yl]amino } -1,3-thiazole-5-carbonitrile HN O NN N N N 10 H or a pharmaceutically acceptable salt or stereoisomer thereof.

11. A compound which is: 2-({ 6-[(1, 1-dioxidotetrahydrothien-3-yl)aminol-2-methylpyrimdin-4-yl I amino)- 1,3-thiazole-5 15 carbonitrile 0 0 S NH N N CN H -94- WO 2004/041164 PCT/US2003/034100 or a pharmaceutically acceptable salt or stereoisomer thereof.

12. A compound which is: 2-{ [ 2 -methyl- 6 -(tetrahydrofuran-3-ylamino)pyrimidin-4-yl]amino }-1,3-thiazole-5-carbonitrile 0 NH N N N N S N 5 H or a pharmaceutically acceptable salt or stereoisomer thereof.

13. A compound which is: 2-{[ 2 -isopropyl-6-(piperidin-4-yloxy)pyrimidin-4-yl]amino}-1,3-thiazole-5-carbonitrile HN N CN T-1N N S C 10 H or a pharmaceutically acceptable salt thereof.

14. A pharmaceutical composition which is comprised of a compound in accordance with Claim 1 and a pharmaceutically acceptable carrier. 15

15. A method of treating or preventing cancer in a mammal in need of such treatment which is comprised of administering to said mammal a therapeutically effective amount of a compound of Claim 1. 20

16. A method of treating or preventing cancer in accordance with Claim 15 wherein the cancer is selected from cancers of the brain, genitourinary tract, lymphatic system, stomach, larynx, and lung. - 95 - WO 2004/041164 PCT/US2003/034100

17. A method of treating or preventing cancer in accordance with Claim 15 wherein the cancer is selected from histiocytic lymphoma, lung adenocarcinoma, small cell lung cancers, pancreatic cancer, glioblastomas and breast carcinoma. 5

18. A method of treating or preventing cancer in accordance with Claim 15 wherein the cancer is selected from colorectal cancer, prostate cancer, breast cancer, and lung cancer. 10

19. A method of treating or preventing a disease in which angiogenesis is implicated, which is comprised of administering to a mammal in need of such treatment a therapeutically effective amount of a compound of Claim 1.

20. A method in accordance with Claim 19 wherein the disease is an ocular 15 disease.

21. A method of treating or preventing retinal vascularization which is comprised of administering to a mammal in need of such treatment a therapeutically effective amount of compound of Claim 1. 20

22. A method of treating or preventing diabetic retinopathy which is comprised of administering to a mammal in need of such treatment a therapeutically effective amount of compound of Claim 1. 25

23. A method of treating or preventing age-related macular degeneration which is comprised of administering to a mammal in need of such treatment a therapeutically effective amount of a compound of Claim 1.

24. The method of Claim 19 further comprising the use of photodynamic 30 therapy with a photosensitive drug.

25. The method of Claim 24 wherein the photosensitive drug is verteoporfin.

26. The method of Claim 24 wherein the disease is age-related macular 35 degeneration. -96- WO 2004/041164 PCT/US2003/034100

27. A method of treating or preventing inflammatory diseases which comprises administering to a mammal in need of such treatment a therapeutically effective amount of a compound of Claim 1. 5

28. A method according to Claim 27 wherein the inflammatory disease is selected from rheumatoid arthritis, psoriasis, contact dermatitis and delayed hypersensitivity reactions. 10

29. A method of treating or preventing a tyrosine kinase-dep6ndent disease or condition which comprises administering a therapeutically effective amount of a compound of Claim 1.

30. A pharmaceutical composition made by combining the compound of 15 Claim 1 and a pharmaceutically acceptable carrier.

31. A process for making a pharmaceutical composition which comprises combining a compound of Claim 1 with a pharmaceutically acceptable carrier. 20

32. A method of treating or preventing bone associated pathologies selected from osteosarcoma, osteoarthritis, and rickets which comprises administering a therapeutically effective amount of a compound of Claim 1.

33. The composition of Claim 14 further comprising a second compound 25 selected from: 1) an estrogen receptor modulator, 2) an androgen receptor modulator, 3) retinoid receptor modulator, 4) a cytotoxic agent, 30 5) an antiproliferative agent, 6) a prenyl-protein transferase inhibitor, 7) an HMG-CoA reductase inhibitor, 8) an HIV protease inhibitor, 9) a reverse transcriptase inhibitor, 35 10) another angiogenesis inhibitor, and - 97 - WO 2004/041164 PCT/US2003/034100 11) a PPAR-y agonist.

34. The composition of Claim 33, wherein the second compound is another angiogenesis inhibitor selected from the group consisting of a tyrosine kinase inhibitor, an 5 inhibitor of epidermal-derived growth factor, an inhibitor of fibroblast-derived growth factor, an inhibitor of platelet derived growth factor, an IMP inhibitor, an integrin blocker, interferon-a, interleukin-12, pentosan polysulfate, a cyclooxygenase inhibitor, carboxyamidotriazole, combretastatin A-4, squalamine, 6-(O-chloroacetyl-carbonyl)-fumagillol, thalidomide, angiostatin, troponin-1, and an antibody to VEGF. 10

35. The composition of Claim 33, wherein the second compound is an estrogen receptor modulator selected from tamoxifen and raloxifene.

36. The composition of Claim 14 further comprising a steroidal anti 15 inflammatory compound.

37. The composition of Claim 14 further comprising an anti-hypertensive compound. 20

38. A method of treating cancer which comprises administering a therapeutically effective amount of a compound of Claim 1 in combination with radiation therapy.

39. A method of treating or preventing cancer which comprises administering 25 a therapeutically effective amount of a compound of Claim 1 in combination with a compound selected from: 1) an estrogen receptor modulator, 2) an androgen receptor modulator, 3) retinoid receptor modulator, 30 4) a cytotoxic agent, 5) an antiproliferative agent, 6) a prenyl-protein transferase inhibitor, 7) an HMG-CoA reductase inhibitor, 8) an H protease inhibitor, 35 9) a reverse transcriptase inhibitor, and -98- WO 2004/041164 PCT/US2003/034100 10) another angiogenesis inhibitor.

40. A method of treating cancer which comprises administering a therapeutically effective amount of a compound of Claim 1 in combination with radiation 5 therapy and a compound selected from: 1) an estrogen receptor modulator, 2) an androgen receptor modulator, 3) retinoid receptor modulator, 4) a cytotoxic agent, 10 5) an antiproliferative agent, 6) a prenyl-protein transferase inhibitor, 7) an HMG-CoA reductase inhibitor, 8) an IV protease inhibitor, 9) a reverse transcriptase inhibitor, and 15 10) another angiogenesis inhibitor.

41. A method of treating or preventing cancer which comprises administering a therapeutically effective amount of a compound of Claim 1 and paclitaxel or trastuzumab. 20

42. A method of treating or preventing cancer which comprises administering a therapeutically effective amount of a compound of Claim 1 and a GPIb/I]Ia antagonist.

43. The method of Claim 42 wherein the GPIlb/Ila antagonist is tirofiban. 25

44. A method of reducing or preventing tissue damage following a cerebral ischemic event which comprises administering a therapeutically effective amount of a compound of Claim 1.

45. A method of treating or preventing cancer which comprises administering 30 a therapeutically effective amount of a compound of Claim 1 in combination with a COX-2 inhibitor.

46. A method of treating or preventing preeclampsia which comprises administering a therapeutically effective amount of a compound of Claim 1. 35 - 99 - WO 2004/041164 PCT/US2003/034100

47. A method of treating or preventing tissue damage due to bacterial meningitis which comprises administering a therapeutically effective amount of a compound of Claim 1. 5

48. A method to treat or prevent endometriosis which comprises administering a therapeutically effective amount of a compound of Claim 1.

49. A method of treating or preventing diabetic retinopathy which comprises administering a therapeutically effective amount of a compound of Claim 1 in combination with 10 a PPAR-y agonist.

50. A method of treating acute myeloid leukemia which comprises administering a therapeutically effective amount of a compound of Claim 1. 15

51. A method of treating cancer which comprises administering a therapeutically effective amount of a compound of Claim 1 in combination with gene therapy.

52. A method of treating or preventing cancer which comprises administering a therapeutically effective amount of a compound which is a dual inhibitor of tyrosine kinase and 20 cyclin dependent kinase.

53. The method of Claim 52 wherein the dual inhibitor is selected from: N-(tert-Butyl)-2-{4-[({ 6-[(5-cyano-1,3-thiazol-2-yl)amino]-2-methylpyrimidin-4 yl}oxy)methyl]piperidin-1-yl}acetamide; 25 2-{ [2-methyl-6-(piperidin-4-yloxy)pyrimidin-4-ylJamino }-1,3-thiazole-5-carbonitrile; 2-{ [2-methyl-6-(piperidin-4-ylmethoxy)pyrimidin-4-yl] amino }-1 ,3-thiazole-5-carbonitrile; and 2-{ [ 2 -isopropyl-6-(piperidin-4-yloxy)pyrimidin-4-yl]amino }-1,3-thiazole-5-carbonitrile; or a pharmaceutically acceptable salt thereof.

54. A method of treating or preventing ovarian hyperstimulation syndrome 30 which is comprised of administering to a mammal in need of such treatment a therapeutically effective amount of a compound of Claim 1.

55. A method of treating or preventing ovarian hyperstimulation syndrome which comprises administering a therapeutically effective amount of a compound of Claim 1 in 35 combination with an ovulation stimulator. - 100- WO 2004/041164 PCT/US2003/034100

56. The method of Claim 55 wherein the ovulation stimulator is selected from bromocriptine, luprolide, clonifene and pharmaceutically acceptable salts thereof, follicle stimulating hormone, menopausal gonadotropin or mentropins, chorionic gonadotropin, 5 luteinizing hormone releasing hormone, luteinizing hormone and combinations thereof.

57. A method according to Claim 20 wherein the ocular disease is macular edema. - 101 -