AU2003267185A1 - Polyhydroxyalkanoate production by coenzyme a-dependent aldehyde dehydrogenase pathways - Google Patents
Polyhydroxyalkanoate production by coenzyme a-dependent aldehyde dehydrogenase pathwaysInfo
- Publication number
- AU2003267185A1 AU2003267185A1 AU2003267185A AU2003267185A AU2003267185A1 AU 2003267185 A1 AU2003267185 A1 AU 2003267185A1 AU 2003267185 A AU2003267185 A AU 2003267185A AU 2003267185 A AU2003267185 A AU 2003267185A AU 2003267185 A1 AU2003267185 A1 AU 2003267185A1
- Authority
- AU
- Australia
- Prior art keywords
- coa
- organism
- aldehyde dehydrogenase
- dependent aldehyde
- pha
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108020002663 Aldehyde Dehydrogenase Proteins 0.000 title abstract 6
- 230000001419 dependent effect Effects 0.000 title abstract 6
- 102000005369 Aldehyde Dehydrogenase Human genes 0.000 title abstract 5
- 239000005014 poly(hydroxyalkanoate) Substances 0.000 title abstract 5
- 229920000903 polyhydroxyalkanoate Polymers 0.000 title abstract 3
- 230000037361 pathway Effects 0.000 title abstract 2
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 title 1
- 229940093530 coenzyme a Drugs 0.000 title 1
- 239000000178 monomer Substances 0.000 abstract 3
- 229920000642 polymer Polymers 0.000 abstract 3
- REKYPYSUBKSCAT-UHFFFAOYSA-N 3-hydroxypentanoic acid Chemical compound CCC(O)CC(O)=O REKYPYSUBKSCAT-UHFFFAOYSA-N 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- HPMGFDVTYHWBAG-UHFFFAOYSA-N 3-hydroxyhexanoic acid Chemical compound CCCC(O)CC(O)=O HPMGFDVTYHWBAG-UHFFFAOYSA-N 0.000 abstract 1
- ALRHLSYJTWAHJZ-UHFFFAOYSA-M 3-hydroxypropionate Chemical compound OCCC([O-])=O ALRHLSYJTWAHJZ-UHFFFAOYSA-M 0.000 abstract 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 1
- FMHKPLXYWVCLME-UHFFFAOYSA-N 4-hydroxy-valeric acid Chemical compound CC(O)CCC(O)=O FMHKPLXYWVCLME-UHFFFAOYSA-N 0.000 abstract 1
- SJZRECIVHVDYJC-UHFFFAOYSA-M 4-hydroxybutyrate Chemical compound OCCCC([O-])=O SJZRECIVHVDYJC-UHFFFAOYSA-M 0.000 abstract 1
- PHOJOSOUIAQEDH-UHFFFAOYSA-N 5-hydroxypentanoic acid Chemical compound OCCCCC(O)=O PHOJOSOUIAQEDH-UHFFFAOYSA-N 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 206010064571 Gene mutation Diseases 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 101150041588 eutE gene Proteins 0.000 abstract 1
- 108010010718 poly(3-hydroxyalkanoic acid) synthase Proteins 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Prostheses (AREA)
- Enzymes And Modification Thereof (AREA)
- Adhesives Or Adhesive Processes (AREA)
Abstract
The present invention provides a method of producing polyhydroxyalkanoate (PHA) polymers comprising at least one hydroxyacyl monomer selected from the group consisting of 3-hydroxypropionate, 3-hydroxyvalerate, 4-hydroxybutyrate, 4-hydroxyvalerate, 5-hydroxyvalerate, and 3-hydroxyhexanoate, comprising: expressing in an organism genes encoding a polyhydroxyalkanoate (PHA) synthase and a CoA-dependent aldehyde dehydrogenase, feeding a chemical that is converted by the organism to at least one hydroxyacyl-CoA monomer by an enzyme-catalysed pathway that includes the CoA-dependent aldehyde dehydrogenase activity and allowing polymerisation of the at least one hydroxyacyl-CoA monomer by the PHA synthase to form PHA polymers, and recovering the thus-produced PHA polymers, wherein the organism is produced from a wild-type organism having an endogenous CoA-dependent aldehyde dehydrogenase gene which is modified by gene mutation to increase CoA-dependent aldehyde dehydrogenase activity, or wherein the organism is a recombinant organism in which a gene is recombinantly expressed to encode the CoA-dependent aldehyde dehydrogenase. The present invention also provides a recombinant organism for use in the foregoing method. The present invention also provides a method comprising the step of amplifying a DNA sequence encoding the eutE gene from an E. coli genome using the following oligonucleotides primers: 5' GGT GGT ACC TTA AGA GGA GGT TTT TAT GAA TCA ACA GGA TAT TGA ACA-3' (SEQ ID NO: 1) and 5'-GGT GCG GCC GCT TAA ACA ATG CGA AAC GCA TCG-3' (SEQ ID NO: 2).
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US41008702P | 2002-09-12 | 2002-09-12 | |
| US60/410,087 | 2002-09-12 | ||
| PCT/US2003/028842 WO2004024876A2 (en) | 2002-09-12 | 2003-09-12 | Polyhydroxyalkanoate production by coenzyme a-dependent aldehyde dehydrogenase pathways |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2003267185A1 true AU2003267185A1 (en) | 2004-04-30 |
| AU2003267185A8 AU2003267185A8 (en) | 2004-04-30 |
Family
ID=31994056
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2003267185A Abandoned AU2003267185A1 (en) | 2002-09-12 | 2003-09-12 | Polyhydroxyalkanoate production by coenzyme a-dependent aldehyde dehydrogenase pathways |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US8071355B2 (en) |
| EP (2) | EP2365088B1 (en) |
| JP (2) | JP4628103B2 (en) |
| AT (1) | ATE505559T1 (en) |
| AU (1) | AU2003267185A1 (en) |
| DE (2) | DE10075400T8 (en) |
| ES (1) | ES2395053T3 (en) |
| WO (1) | WO2004024876A2 (en) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100979694B1 (en) | 2005-05-24 | 2010-09-02 | 한국과학기술원 | Cells or Plants Having an Producing Ability of Polylactate or Its Copolymers and Method for Preparing Polylactate or Its Copolymers Using the Same |
| TWI568847B (en) | 2007-03-16 | 2017-02-01 | 奇諾麥提卡公司 | Compositions and methods for the biosynthesis of 1,4-butanediol and its precursors |
| US7947483B2 (en) | 2007-08-10 | 2011-05-24 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of 1,4-butanediol |
| WO2009157702A2 (en) * | 2008-06-24 | 2009-12-30 | (주)Lg화학 | Method for preparing polylactate and copolymer thereof using a mutant microorganism with enhanced polylactate, and the copolymer producing capability thereof |
| BRPI0918483A2 (en) | 2008-09-10 | 2020-07-14 | Genomatica, Inc | non-naturally occurring microbial organism, and method of producing a compound |
| US20100184173A1 (en) * | 2008-11-14 | 2010-07-22 | Genomatica, Inc. | Microorganisms for the production of methyl ethyl ketone and 2-butanol |
| KR20110104492A (en) | 2008-12-12 | 2011-09-22 | 메타볼릭스 인코포레이티드 | Green process and compositions for producing poly(5hv)and 5 carbon chemicals |
| KR101293886B1 (en) * | 2009-02-05 | 2013-08-07 | 주식회사 엘지화학 | Recombinant Ralstonia eutropha Having an Producing Ability of Polylactate or Its Copolymers and Method for Preparing Polylactate or Its Copolymers Using the Same |
| JP5964747B2 (en) | 2009-06-04 | 2016-08-03 | ゲノマチカ， インク． | Microorganisms and related methods for the production of 1,4-butanediol |
| VN29235A1 (en) * | 2009-06-04 | 2012-03-26 | ||
| CA2777459A1 (en) * | 2009-10-13 | 2011-04-21 | Genomatica, Inc. | Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine and related compounds, and methods related thereto |
| US8530210B2 (en) | 2009-11-25 | 2013-09-10 | Genomatica, Inc. | Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone |
| US8637286B2 (en) | 2010-02-23 | 2014-01-28 | Genomatica, Inc. | Methods for increasing product yields |
| US8048661B2 (en) * | 2010-02-23 | 2011-11-01 | Genomatica, Inc. | Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes |
| FR2964014B1 (en) * | 2010-08-31 | 2013-04-05 | Ifremer | NUCLEUS COATED WITH A FILMOGENEOUS COATING WITH ANTIBACTERIAL AND CICATRISANT PROPERTIES AND METHOD OF OBTAINING THE SAME |
| FR2968506B1 (en) * | 2010-12-14 | 2013-02-08 | Ifremer | NUCLEUS COVERED WITH PHA |
| CN102212501B (en) * | 2011-03-31 | 2013-06-12 | 山东大学 | Recombinant escherichia coli and method for applying same to produce poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) by utilizing single carbon source |
| EP2702138A2 (en) * | 2011-04-29 | 2014-03-05 | Metabolix, Inc. | Green process for producing polyhydroxyalkanoates and chemicals using a renewable feedstock |
| AU2012299191B2 (en) | 2011-08-19 | 2016-03-03 | Genomatica, Inc. | Microorganisms and methods for producing 1,3-butanediol and related alcohols |
| BR112014010448A2 (en) | 2011-11-02 | 2017-04-18 | Genomatica Inc | microorganisms and methods for caprolactone production |
| BR112014030202A2 (en) | 2012-06-04 | 2017-09-12 | Genomatica Inc | microorganisms and methods for the production of 4-hydroxybutyrate 1,4-butanediol and related compounds |
| WO2014062564A1 (en) | 2012-10-15 | 2014-04-24 | Genomatica, Inc. | Microorganisms and methods for production of specific length fatty alcohols and related compounds |
| US20160040172A1 (en) | 2013-03-15 | 2016-02-11 | Genomatica, Inc. | Microorganisms and methods for producing butadiene and related compounds by formate assimilation |
| WO2015077752A1 (en) | 2013-11-25 | 2015-05-28 | Genomatica, Inc. | Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol |
| EP3167066A4 (en) | 2014-07-11 | 2018-03-07 | Genomatica, Inc. | Microorganisms and methods for the production of butadiene using acetyl-coa |
| US20170298363A1 (en) | 2014-09-18 | 2017-10-19 | Genomatica, Inc | Non-natural microbial organisms with improved energetic efficiency |
| CN108026214B (en) | 2015-05-30 | 2021-03-02 | 基因组股份公司 | Vinyl isomerase-dehydratase, enol-dehydratase, linalool-dehydratase and/or crotyl alcohol dehydratase, and preparation and use thereof |
| JP7250025B2 (en) | 2017-10-26 | 2023-03-31 | ノロオ アイシー シーオー．エルティーディー． | Production and separation of 3-hydroxypropionic acid |
| US11566250B2 (en) | 2017-10-26 | 2023-01-31 | Noroo Ic Co., Ltd. | Production and separation of 3-hydroxypropionic acid |
| JP7209434B2 (en) * | 2019-10-31 | 2023-01-20 | 株式会社カネカ | Method for producing polyhydroxyalkanoic acid and use thereof |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4477654A (en) | 1981-07-07 | 1984-10-16 | Imperial Chemical Industries Plc | 3-Hydroxybutyrate polymers |
| EP0145233B2 (en) * | 1983-11-23 | 1991-11-06 | Imperial Chemical Industries Plc | Separation processfor a 3-hydroxybutyrate polymer |
| US5245023A (en) * | 1987-06-29 | 1993-09-14 | Massachusetts Institute Of Technology | Method for producing novel polyester biopolymers |
| US5250430A (en) * | 1987-06-29 | 1993-10-05 | Massachusetts Institute Of Technology | Polyhydroxyalkanoate polymerase |
| US5480794A (en) * | 1987-06-29 | 1996-01-02 | Massachusetts Institute Of Technology And Metabolix, Inc. | Overproduction and purification of soluble PHA synthase |
| US5229279A (en) | 1987-06-29 | 1993-07-20 | Massachusetts Institute Of Technology | Method for producing novel polyester biopolymers |
| GB9325952D0 (en) * | 1993-12-20 | 1994-02-23 | Zeneca Ltd | Process for preparing films and coatings |
| ZA95627B (en) | 1994-01-28 | 1995-10-05 | Procter & Gamble | Biodegradable copolymers and plastic articles comprising biodegradable copolymers |
| MX9603065A (en) * | 1994-01-28 | 1997-06-28 | Procter & Gamble | Biodegradable 3-polyhydroxybutyrate/3-polyhydroxyhexanoate copolymer films. |
| FR2719012B1 (en) | 1994-04-22 | 1996-07-12 | Pomagalski Sa | Detachable clamp of a vehicle towed by cable. |
| US5563239A (en) * | 1994-11-09 | 1996-10-08 | Eastman Chemical Company | Composition and process for the production of poly(3-hydroxyalkanoates) |
| ATE297469T1 (en) * | 1997-03-03 | 2005-06-15 | Metabolix Inc | METHOD FOR POLYESTERN BIOSYNTHESIS |
| EP1015565B1 (en) | 1997-09-19 | 2006-04-12 | Metabolix, Inc. | Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids |
| US7455999B2 (en) | 1998-01-22 | 2008-11-25 | Metabolix, Inc. | Transgenic systems for the manufacture of poly (3-hydroxy-butyrate-co-3-hydroxyhexanoate) |
| ES2302386T3 (en) | 1998-08-04 | 2008-07-01 | Metabolix, Inc. | PRODUCTION OF POLYHYDROXIALCANOATES FROM POLYOLS. |
| DE69930276T2 (en) * | 1998-08-18 | 2006-10-05 | Metabolix, Inc., Cambridge | Transgenic polyhydroxyalkanoate producing microbes |
| EP2305324B1 (en) * | 1999-03-25 | 2014-09-17 | Metabolix, Inc. | Medical devices and applications of polyhydroxyalkanoate polymers |
| AU7599601A (en) * | 2000-07-21 | 2002-02-05 | Metabolix Inc | Production of polyhydroxyalkanoates from polyols |
| ES2362405T3 (en) * | 2000-10-27 | 2011-07-04 | Metabolix, Inc. | COMPOSITIONS THAT INCLUDE POLYHYDROXIALCANOATS AND REACTIVE MONOMERS. |
-
2003
- 2003-09-12 WO PCT/US2003/028842 patent/WO2004024876A2/en active Search and Examination
- 2003-09-12 ES ES10075400.1T patent/ES2395053T3/en not_active Expired - Lifetime
- 2003-09-12 JP JP2004536287A patent/JP4628103B2/en not_active Expired - Lifetime
- 2003-09-12 EP EP10075400.1A patent/EP2365088B1/en not_active Expired - Lifetime
- 2003-09-12 AU AU2003267185A patent/AU2003267185A1/en not_active Abandoned
- 2003-09-12 DE DE10075400T patent/DE10075400T8/en active Active
- 2003-09-12 EP EP03749656A patent/EP1581619B1/en not_active Expired - Lifetime
- 2003-09-12 US US10/661,939 patent/US8071355B2/en active Active
- 2003-09-12 DE DE60336757T patent/DE60336757D1/en not_active Expired - Lifetime
- 2003-09-12 AT AT03749656T patent/ATE505559T1/en not_active IP Right Cessation
-
2010
- 2010-06-16 JP JP2010137010A patent/JP2010268797A/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| DE10075400T1 (en) | 2012-10-11 |
| ES2395053T3 (en) | 2015-10-08 |
| EP2365088A2 (en) | 2011-09-14 |
| ES2395053T1 (en) | 2013-02-07 |
| ATE505559T1 (en) | 2011-04-15 |
| DE60336757D1 (en) | 2011-05-26 |
| JP2006507811A (en) | 2006-03-09 |
| US20040106176A1 (en) | 2004-06-03 |
| EP2365088B1 (en) | 2015-05-27 |
| EP1581619A2 (en) | 2005-10-05 |
| JP4628103B2 (en) | 2011-02-09 |
| EP1581619A4 (en) | 2007-02-14 |
| EP2365088A3 (en) | 2012-01-11 |
| WO2004024876A2 (en) | 2004-03-25 |
| US8071355B2 (en) | 2011-12-06 |
| JP2010268797A (en) | 2010-12-02 |
| EP1581619B1 (en) | 2011-04-13 |
| WO2004024876A3 (en) | 2006-05-26 |
| DE10075400T8 (en) | 2013-04-25 |
| AU2003267185A8 (en) | 2004-04-30 |
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