AU2003260538A1 - Method for the functional artificial evolution of polynucleotide sequences, recombinant polynucleotides obtained and peptides, polypeptides and proteins coded by same - Google Patents

Method for the functional artificial evolution of polynucleotide sequences, recombinant polynucleotides obtained and peptides, polypeptides and proteins coded by same

Info

Publication number
AU2003260538A1
AU2003260538A1 AU2003260538A AU2003260538A AU2003260538A1 AU 2003260538 A1 AU2003260538 A1 AU 2003260538A1 AU 2003260538 A AU2003260538 A AU 2003260538A AU 2003260538 A AU2003260538 A AU 2003260538A AU 2003260538 A1 AU2003260538 A1 AU 2003260538A1
Authority
AU
Australia
Prior art keywords
polypeptides
peptides
same
polynucleotide sequences
recombinant polynucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003260538A
Other languages
English (en)
Other versions
AU2003260538A8 (en
Inventor
Michele Legrain
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Entomed AB
Original Assignee
Entomed AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Entomed AB filed Critical Entomed AB
Publication of AU2003260538A1 publication Critical patent/AU2003260538A1/en
Publication of AU2003260538A8 publication Critical patent/AU2003260538A8/xx
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4723—Cationic antimicrobial peptides, e.g. defensins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2003260538A 2002-05-06 2003-05-06 Method for the functional artificial evolution of polynucleotide sequences, recombinant polynucleotides obtained and peptides, polypeptides and proteins coded by same Abandoned AU2003260538A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR02/05642 2002-05-06
FR0205642A FR2839317A1 (fr) 2002-05-06 2002-05-06 Procede d'evolution fonctionnelle artificielle de sequences polynucleotidiques, polynucleotides recombines obtenus et peptides, polypeptides et proteines codes par ceux-ci
PCT/FR2003/001405 WO2003097833A2 (fr) 2002-05-06 2003-05-06 Procede d'evolution fonctionnelle artificielle de sequences polyneucleotidiques, polynucleotides recombines obtenus et peptides, polypeptides et proteines codes par ceux-ci

Publications (2)

Publication Number Publication Date
AU2003260538A1 true AU2003260538A1 (en) 2003-12-02
AU2003260538A8 AU2003260538A8 (en) 2003-12-02

Family

ID=29226215

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003260538A Abandoned AU2003260538A1 (en) 2002-05-06 2003-05-06 Method for the functional artificial evolution of polynucleotide sequences, recombinant polynucleotides obtained and peptides, polypeptides and proteins coded by same

Country Status (3)

Country Link
AU (1) AU2003260538A1 (fr)
FR (1) FR2839317A1 (fr)
WO (1) WO2003097833A2 (fr)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5286632A (en) * 1991-01-09 1994-02-15 Jones Douglas H Method for in vivo recombination and mutagenesis
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination

Also Published As

Publication number Publication date
WO2003097833A3 (fr) 2004-04-01
WO2003097833A2 (fr) 2003-11-27
FR2839317A1 (fr) 2003-11-07
AU2003260538A8 (en) 2003-12-02

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase