AU2003236660A1 - Method for parallel preparation of rna and proteins from a tissue sample - Google Patents

Method for parallel preparation of rna and proteins from a tissue sample

Info

Publication number
AU2003236660A1
AU2003236660A1 AU2003236660A AU2003236660A AU2003236660A1 AU 2003236660 A1 AU2003236660 A1 AU 2003236660A1 AU 2003236660 A AU2003236660 A AU 2003236660A AU 2003236660 A AU2003236660 A AU 2003236660A AU 2003236660 A1 AU2003236660 A1 AU 2003236660A1
Authority
AU
Australia
Prior art keywords
rna
proteins
tissue sample
parallel preparation
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003236660A
Inventor
Thomas Buschmann
Steffen Heim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EUROPROTEOME AG
Original Assignee
EUROPROTEOME AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EUROPROTEOME AG filed Critical EUROPROTEOME AG
Publication of AU2003236660A1 publication Critical patent/AU2003236660A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2003236660A 2002-05-15 2003-05-15 Method for parallel preparation of rna and proteins from a tissue sample Abandoned AU2003236660A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10222494.3 2002-05-15
DE2002122494 DE10222494A1 (en) 2002-05-15 2002-05-15 Parallel recovery of RNA and protein from tissue, useful e.g. for diagnosis of tumors, by treating sample with RNAlater solution, homogenizing and separating epithelial cells
PCT/EP2003/005131 WO2003097874A1 (en) 2002-05-15 2003-05-15 Method for parallel preparation of rna and proteins from a tissue sample

Publications (1)

Publication Number Publication Date
AU2003236660A1 true AU2003236660A1 (en) 2003-12-02

Family

ID=29285617

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003236660A Abandoned AU2003236660A1 (en) 2002-05-15 2003-05-15 Method for parallel preparation of rna and proteins from a tissue sample

Country Status (4)

Country Link
EP (1) EP1546381A1 (en)
AU (1) AU2003236660A1 (en)
DE (1) DE10222494A1 (en)
WO (1) WO2003097874A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012377417B2 (en) * 2012-04-18 2017-04-13 Syngenta Participations Ag Methods and compositions for dual extraction of protein and nucleic acid

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6117985A (en) * 1995-06-16 2000-09-12 Stemcell Technologies Inc. Antibody compositions for preparing enriched cell preparations
GB9518156D0 (en) * 1995-09-06 1995-11-08 Medical Res Council Method of isolating cells
GB9705949D0 (en) * 1997-03-21 1997-05-07 Electrophoretics International Diagnosis of tumours and other abnormalities of body cells
US6204375B1 (en) * 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
US6777210B1 (en) * 1998-09-24 2004-08-17 Ambion, Inc. Method and reagents for inactivating ribonucleases RNase A, RNase I and RNase T1
GB9902302D0 (en) * 1999-02-02 1999-03-24 Larsen Frank Detecting enzyume activity

Also Published As

Publication number Publication date
EP1546381A1 (en) 2005-06-29
WO2003097874A1 (en) 2003-11-27
DE10222494A1 (en) 2003-11-27

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase