AU2003200708B2 - Protein purification - Google Patents

Description

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AUSTRALIA

Patents Act 1990

ORIGINAL

COMPLETE SPECIFICATION STANDARD PATENT Applicant(s): Address for Service: Invention Title: Genentech, Inc.

1 DNA Way South San Francisco California 94080-4990 United States of America DAVIES COLLISON CAVE Patent Trade Mark Attorneys Level 10, 10 Barrack Street SYDNEY NSW 2000 Protein purification The following statement is a full description of this invention, including the best method of performing it known to me:- 5020 I P1241RI PROTEIN PURIFICATION

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o BACKGROUND OF THE INVENTION Field of the Invention r- 5 This invention relates generally to protein purification. In particular, the invention relates to a method for purifying a polypeptide an antibody) from a composition comprising the polypeptide and at least one contaminant using the method of ion exchange chromatography.

Description of Related Art 00 o The large-scale, economic purification of proteins is increasingly an important problem for the 10 biotechnology industry. Generally, proteins are produced by cell culture, using either mammalian or bacterial cell

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O lines engineered to produce the protein of interest by insertion of a recombinant plasmid containing the gene for eCn that protein. Since the cell lines used are living organisms, they must be fed with a complex growth medium, 0 O containing sugars, amino acids, and growth factors, usually supplied from preparations of animal serum.

Separation of the desired protein from the mixture of compounds fed to the cells and from the by-productsof the cells themselves to a purity sufficient for use as a human therapeutic poses a formidable challenge.

Procedures for purification of proteins from cell debris initially depend on the site of expression of the protein. Some proteins can be caused to be secreted directly from the cell into the surrounding growth media; others are made intracellularly. For the latter proteins, the first step of a purification process involves lysis of the cell, which can be done by a variety of methods, including mechanical shear, osmotic shock,' or enzymatic treatments. Such disruption releases the entire contents of the cell into the homogcnatc, and in addition produces subcellular fragments that are difficult to remove due to their small size. These are generally removed by differential centrifugation or by filtration. The same problem arises, although on a smaller scale, with directly secreted proteins due to the natural death of cells and release of intracellular host cell proteins in the course of the protein production run.

Once a clarified solution containing the protein of interest has been obtained, its separation from the other proteins produced by the cell is usually attempted using a combination of different chromatography techniques. These techniques separate mixtures of proteins on the basis of their charge, degree of hydrophobicity, or size. Several different chromatography resins are available for each of these techniques, allowing accurate tailoring of the purification scheme to the particular protein involved. The essence of each of these separation methods is that proteins can be caused either to move at different rates down a long column, achieving a physical separation that increases as they pass further down the column, or to adhere selectively to the separation medium, being then differentially eluted by different solvents. In some cases, the desired protein is separated from impurities when the impurities specifically adhere to the column, and the protein of interest does not, that is, the protein of interest is present in the "flow-through".

Ion exchange chromatography is a chromatographic technique that is commonly used for the purification of proteins. In ion exchange chromatography, charged patches on the surface of the solute are attracted by opposite charges attached to a chromatography matrix, provided the ionic strength of the surrounding buffer is low. Elutionis generally achieved by increasing the ionic strength conductivity) of the buffer to compete with the solute for the charged sites of the ion exchange matrix. Changing the pH and thereby altering the charge of

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P1241R1 the solute is another way to achieve elution of the solute. The change in conductivity or pH may be gradual Cn O (gradient elution) or stepwise (step elution). In the past, these changes have been progressive; the pH or

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C conductivity is increased or decreased in a single direction.

SSUMMARY OF THE INVENTION C4 5 The present invention provides an ion exchange chromatographic method wherein a polypeptide of interest is bound to the ion exchange material at an initial conductivity or pH andthen the ion exchange material is washed with an intermediate buffer at a different conductivity or pH, or both. At a specific point following this intermediate wash, and contrary to ion exchange chromatography standard practice, the ion exchange material O is washed with a wash buffer where the change in conductivity or pH, or both, from the intermediate buffer to O 10 the wash buffer is in an opposite direction to the change in conductivity or pH, or both, achieved in the. previous 0 Ci steps. Only after washing with the wash buffer, is the ion exchange material prepared for the polypeptide O molecule of interest to be eluted by the application of the elution buffer having a conductivity or pH, or both, O which differ from the conductivity or pH, or both, of the buffers used in previous steps.

i This novel approach to ion exchange chromatography is particularly useful in situations where a product molecule must be separated from a very closely related contaminant molecule at full manufacturingscale, where both purity and high recovery of polypeptide product are desired.

Accordingly, the invention provides a method for purifying a polypeptide from a composition comprising the polypeptide and a contaminant, which method comprises the following steps performed sequentially: binding the polypeptide to an ion exchange material using a loading buffer, wherein the loading buffer is at a first conductivity and pH; washing the ion exchange material with an intermediate buffer at a second conductivity and/or pH so as to elute the' contaminant from the ion exchange material; washing the ion exchange material with a wash buffer which is at a third conductivity and/or pH, wherein the change in conductivity and/or pH from the intermediate buffer to the wash buffer is in an opposite direction to the change in conductivity and/or pH from the loading buffer to the intermediate buffer; and washing the ion exchange material with an elution buffer at a fourth conductivity and/or pH so as to elute the polypeptide from the ion exchange material. The first conductivity and/or pH may be the same as the third conductivity and/or pH.

Where the ion exchange material comprises a cation exchange resin, the conductivity and/or pH of the intermediate buffer is/are preferably greater than the conductivity and/or pll of the loading buffer; the 'conductivity and/or pH of the wash buffer is/are preferably less than the conductivity and/or pH of the intermediate buffer; and the conductivity and/or pH of the elution buffer is/are preferably greater.than the conductivity and/or pH of the intermediate buffer. Preferably, the conductivity and/or pH of the wash buffer is/are about the same as the conductivity and/or pH of the loading buffer.

Preferably elution of the contaminant and of the polypeptide is achieved by modifying the conductivity of the intermediate buffer and of the elution buffer, respectively,' while keeping the pH of these buffers approximately the same.

The invention also provides a method for purifying a polypeptide from a composition comprising the 12-12-'06 13:27 FROM-DCC SYDNEY +61292621080 T-154 P006/010 F-139 polypcptide:and a contaminant, which method comprises the following steps performed sequentially; O binding the polypeptide to a cation exchange material using a loading buffer, wherein the loading

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Sbuffer is at a first conductivity and pH; C washing the cation exchange material with an intermediate buffer at a second conductivity and/or S 5 pH which is greater than that of the loading buffer so as to elute the contaminant from the ion exchange material; 0 washing the cation exchange material with a wash buffer which is at a third conductivity and/or pH Cl1 which is less than that of the intermediate buffer; and washing the cation exchange material with an elution buffer at a fourth conductivity and/or pH which is greater than that of the intermediate buffer so as to elute the polypeptide from the ion exchange material.

00 10 In addition, the invention provides a method for purifying an antibody from a composition comprising Sthe antibody and a contaminant, which method comprises loading the composition onto a cation exchange resin, o wherein the amount of antibody loaded onto the cation exchange resin is from about 20mg to about 35mg of the antibody per mL of cation exchange resin and, optionally, further comprising eluting the antibody from the cation O exchange resin. The method preferably further comprises an intermediate wash step for eluting one or more S 15 contaminants from the ion exchange resin. This intermediate wash step usually precedes the step of eluting the antibody.

The invention further provides a composition comprising a mixture of anti-HER2 antibody and one or more acidic variants thereof, wherein the amount of the acidic variant(s) in the composition is less than about and preferably less than about 20%, e.g. in the range from about 1% to about 18%. Optionally, the composition further comprises a pharmaceutically acceptable carrier.

A further aspect of the invention provides a composition comprising a mixture of anti-HER2 antibody and one or more acidic variants thereof, wherein the amount of the acidic variant(s) is less than about 25%, and wherein the acidic variant(s) are predominately deamidated variants wherein one or more asparagine residues of the anti-HER2 antibody have been deamidated, and wherein the anti-HER2 antibody is humMAb4D5-8, and wherein the deamidated variants have Asn30 in CDRI of either or both VL regions of humMAb4D5-8 converted to aspartate.

Brief Description Of The Drawings Figure I is a flow diagram showing how one could perform cation exchange chromatography by altering conductivity to the NaCI concentrations of Example I below) or by altering pH to the pH values as shown in the flow diagram).

Figure 2 is a flow diagram showing how one could perform anion exchange chromatography by altering conductivity to the NaCI concentrations as depicted in the figure) or by altering pH to the pH values as shown).

Figure 3 is an absorbance trace from a cation exchange chromatography run of Example I at full manufacturing scale. Points at which the column is washed with the differentbuffers described herein are marked with arrows.

3 COMS ID No: SBMI-05641477 Received by IP Australia: Time 14:31 Date 2006-12-12 12-12-'06 13:27 FROM-DCC SYDNEY +61292621080 T-154 P007/010 F-239

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SFigure 4 depicts recombinant humanized anti-HER2 monoclonal antibody (rhuMAb HER2) recovered Sin each chromatography fraction (calculated as the percentage of the sum total of all fractions of the relevant o chromatography). Flow through, wash steps, and prepool fractions are all effluent samples collected from the Sonset of load to the initiation of pooling. The pool fraction is the five column volume effluent sample of elution starting at the leading shoulder's inflection point. The regeneration fraction contains effluent captured from the end of pooling to the end of regeneration.

Figure 5 shows the quality of rhuMAb HER2 in each cation exchange chromatography pool sample as 00 evaluated by carboxy sulfon cation exchange high pressure liquid chromatography(CSx HPIEX). Peaks a, b, and I are deamidated forms of rhuMAb HER2. Peak 3 is nondeamidated rhuMAb HER2. Peak 4 is a combination o io 0 c3 3a COMS ID No: SBMI-05641477 Received by IP Australia: Time 14:31 Date 2006-12-12 P1241RI of C-terminal Lysine containing and iso-aspartate variants of rhuMAb HER2.

Ce O Figure 6 shows the absorbance(280 nm) profilesof the 0.025 M MES 0.070 M NaCI, pH 5.6 wash for

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each chromatography. The mass of rhuMAb HER2 applied to the cation exchange resin effects the peak's absorbance level at the apex as well as the amount of buffer required to reach the apex. Due to minor peaks which A 5 occur (as best seen in the 30 mg/mL load) in this wash, the apex is defined as absorbance levels of at least absorbance units (AU).

Figures 7A and 7B show the amino acid sequences of humMAb4D5-8 light chain (SEQ ID NO: 1) and humMAb4DS-8 heavy chain (SEQ ID NO:2), respectively.

o Detailed Description Of The Preferred Embodiments O 10 Definitions: Cl The "composition" to be purified herein comprises the polypeptide of interest and one or more Ce o contaminants. The composition may be "partially purified" having been subjected to one or more purification steps, such as Protein A Chromatography as in Example 1 below) or may be obtained directly from a host cell or organism producing the polypeptide the composition may comprise harvested cell culture fluid).

As used herein, "polypeptide" refers generally to peptides and proteins having more than about ten amino acids. Preferably, the polypeptide is a mammalian protein, examples of which include renin; a growth hormone, including human growth hormone and bovine growth hormone; growth hormone releasing factor; parathyroid hormone; thyroid stimulating hormone; lipoproteins; alpha-1 -antitrypsin; insulin A-chain; insulin Bchain; proinsulin; follicle stimulating hormone; calcitonin; luteinizing hormone; glucagon; cloning factors such as factor VIIIC, factor IX, tissue factor, and von Willebrands factor; anti-clotting factors such as Protein C; atrial natriuretic factor; lung surfactant; a plasminogen activator, such as urokinase or human urine or tissue-type plasminogen activator bombesin; thrombin; hemopoietic growth factor; tumor necrosis factor-alpha and -beta; enkephalinase; RANTES (regulated on activation normally T-cell expressed and secreted); human macrophage inflammatory protein (MIP- -alpha); a serum albumin such as human serum albumin; Muellerianinhibiting substance; relaxin A-chain; relaxin B-chain; prorelaxin; mouse gonadotropin-associated peptide; a microbial protein, such as beta-lactamase; DNase; IgE; a cytotoxic T-lymphocyte associated antigen (CTLA), such as CTLA-4; inhibin; activin; vascular endothelial growth factor (VEGF); receptors for hormones or growth factors; Protein A or D; rheumatoid factors; a neurotrophic factor such as bone-derived neurotrophic factor (BDNF), neurotrophin-3, or -6 (NT-3, NT-4, NT-5, or NT-6), or a nerve growth factor such as NGF-p; platelet-derivedgrowth factor (PDGF); fibroblast growth factor such as aFGF and bFGF; epidermal growth factor (EGF); transforming growth factor (TGF) such as TGF-alpha and TGF-beta, including TGF-01, TGF-02, TGF- P3, TGF-P4, or TGF-P5; insulin-like growth factor-I and -II (IGF-I and IGF-II); des(l-3)-IGF-I (brain IGF-I), insulin-like growth factor binding proteins (IGFBPs); CD proteins such as CD3, CD4, CD8, CDI9 and erythropoietin; osteoinductive factors; immunotoxins; a bone morphogenetic protein (BMP); an interferon such as interferon-alpha, -beta, and -gamma; colony stimulating factors (CSFs), M-CSF, GM-CSF, and G-CSF; interleukins (ILs), IL-1 to IL-10; superoxide dismutase; T-cell receptors; surface membrane proteins; decay accelerating factor; viral antigen such as, for example, a portion of the AIDS envelope; transport proteins; homing receptors; addressins; regulatory proteins; integrins such as CD1 la, CDl lb, CDI Ic, CD18, an ICAM, VLA-4 and VCAM; a tumor associated antigen such as HER2, HER3 or HER4 receptor; and fragments and/or variants

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P1241R1 of any of the above-listed polypeptides. Most preferred is a full length antibody that binds human HER2.

Ce O A "contaminant" is a material that is different from the desired polypeptide product. The contaminant 0 may be a variant of the desired polypeptide a deamidated variant or an amino-aspartate variant of the desired polypeptide) or another polypeptide, nucleic acid, endotoxin etc.

5 A "variant" or "amino acid sequence variant" of a starting polypeptide is a polypeptide that comprises an amino acid sequence different from that of the starting polypeptide. Generally, a variant will possess at least sequence identity, preferably at least 90% sequence identity, more preferablyat least 95% sequence identity, 00 and most preferably at least 98% sequence identity with the native polypeptide. Percentage sequence identity is O determined, for example, by the Fitch et at., Proc. Nol. Acad. Sci. USA 80:1382-1386 (1983), version of the O 10 algorithm describedby Needleman et al., Mol. Biol. 48:443-453(1970), after aligningthe sequences to provide for maximum homology. Amino acid sequence variants of a polypeptide may be prepared by introducing

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O appropriate nucleotide changes into DNA encoding the polypeptide, or by peptide synthesis. Such variants 0 include, for example, deletions from, and/or insertions into and/or substitutions of, residues within the amino acid S sequence of the polypeptide of interest. Any combination of deletion, insertion, and substitution is made to arrive at the final construct, provided that the final construct'possesses the desired characteristics. The amino acid changes also may alter post-translational processes of the polypeptide, such as changing the number or position of glycosylation sites. Methods for generating amino acid sequence variants of polypeptides are described in US Pat 5,534,615, expressly incorporated herein by reference, for example.

An "acidic variant" is a variant of a polypeptide of interest which is more acidic as determined by cation exchange chromatography) than the polypeptide of interest. An example of an acidic variant is a deamidated variant.

A "deamidated" variant of a polypeptide molecule is a polypeptide wherein one or more asparagine residue(s) of the original polypeptide have been converted to aspartate, i.e. the neutral amide side chain has been converted to a residue with an overall acidic character. Deamidated humMAb4D5 antibody from the Example below has Asn30 in CDRI of either or both of the VL regions thereof converted to aspartate. The term "deamidated human DNase" as used herein means human DNase that is deamidatedat the asparagine residue that occurs at position 74 in the amino acid sequence of native mature human DNase (US Patent 5,279,823; expressly incorporated herein by reference).

The term "mixture" as used herein in reference to a composition comprising an anti-HER2 antibody, means the presence of both the desired anti-HER2 antibody and one or more acidic variants thereof The acidic variants may comprise predominantly deamidated anti-HER2 antibody, with minor amounts of other acidic variant(s). It has been found, for example, that in preparationsof anti-HER2 antibody obtained from recombinant expression, as much as about 25% of the anti-HER2 antibody is deamidated.

In preferred embodiments of the invention, the polypeptide is a recombinant polypeptide. A "recombinant polypeptide" is one which has been produced in a host cell which has been transformed or transfected with nucleic acid encoding the polypeptide, or produces the polypeptide as a result of homologous recombination. "Transformation" and "transfection" are used interchangeably to refer to the process of introducing nucleic acid into a cell. Following transformation or transfection, the nucleic acid may integrate into

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P1241RI the host cell genome, or may exist as an extrachromosomalelement. The "host cell" includes a cell in in vitro cell O culture as well a cell within a host animal. Methods for recombinant production of polypeptides are described

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c, in US Pat 5,534,615, expressly incorporated herein by reference, for example.

SThe term "antibody" is used in the broadest sense and specifically covers monoclonal antibodies S 5 (including full length monoclonal antibodies), polyclonal antibodies, multispecific antibodies bispecific antibodies), and antibody fragments so long as they exhibit the desired biological activity.

The antibody herein is directed against an "antigen" of interest. Preferably, the antigen is a biologically important polypeptide and administration of the antibody to a mammal suffering from a disease or disorder can O result in a therapeutic benefit in that mammal. However, antibodies directed against nonpolypeptide antigens O 10 (such as tumor-associated glycolipid antigens; see US Patent 5,091,178)are also contemplated. Where the antigen 0 is a polypeptide, it may be a transmembrane molecule receptor)or ligand such as a growth factor. Exemplary O antigens include those polypeptides discussed above. Preferred molecular targets for antibodies encompassed by S the present invention include CD polypeptides such as CD3, CD4, CD8, CDI9, CD20 and CD34; members of the HER receptor family such as the EGF receptor, HER2, HER3 or HER4 receptor; cell adhesion molecules such as LFA- I, Mac p150,95, VLA-4, ICAM- I, VCAM and av/b3 integrin including either a or b subunits thereof anti-CD1I a, anti-CDI 8Sor anti-CD I b antibodies); growth factors such as VEGF; IgE; blood group antigens; flk2/flt3 receptor; obesity (OB) receptor; mpl receptor; CTLA-4; polypeptide C etc. Soluble antigens or fiagments thereof, optionally conjugated to other molecules, can be used as immunogens for generating antibodies. For transmembrane molecules, such as receptors, fragments of these the extracellular domain of a receptor)can be used as the immunogen. Alternatively, cells expressing the transmembrane molecule can be used as the immunogen. Such cells can be derived from a natural source cancer cell lines) or may be cells which have been transformed by recombinant techniques to express the transmembrane molecule.

The term "monoclonal antibody" as used herein refers to an antibody obtained from a population of substantially homogeneous antibodies, the individual antibodies comprising the population are identical except for possible naturally occurring mutations that may be present in minor amounts. Monoclonal antibodies are highly specific, being directed against a single antigenic site. Furthermore, in contrast to conventional Q (polyclonal) antibody preparations which typically include different antibodies directed against different determinants (epitopes), each monoclonal antibody is directed against a single determinant on the antigen. The modifier "monoclonal" indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies, and is not to be construed as requiring production of the antibody by any particular method. For example, the monoclonal antibodies to be used in accordance with the present invention may be made by the hybridoma method first described by Kohler et Nature 256:495 (1975), or may be made by recombinant DNA methods (see, U.S. Patent No. 4,816,567). In a further embodiment, "monoclonal antibodies"can be isolated from antibody phage libraries generated using the techniques described in McCafferty et al, Nature, 348:552-554 (1990). Clackson et al., Nature, 352:624-628 (1991) and Marks et J. Mol. Biol., 222:581-597 (1991) describe the isolation of murine and human antibodies, respectively, using phage libraries.

Subsequent publications describe the production of high affinity (nM range) human antibodies by chain shuffling (Marks et al., Bio/Technology, 10:779-783 (1992)), as well as combinatorial infection and in vivo recombination as a strategy for constructing very large phage libraries (Waterhouse et al., Nuc. Acids. Res., 21:2265-2266 6

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P1241RI (1993)). Thus, these techniques are viable alternatives to traditional monoclonal antibody hybridoma techniques O for isolation of monoclonal antibodies. Alternatively, it is now possible to produce transgenic animals mice)

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that are capable, upon immunization, of producing a full repertoire of human antibodies in the absence of endogenous immunoglobulin production. For example, it has been described that the homozygous deletion of the antibody heavy-chain joining region (JH) gene in chimeric and germ-line mutant mice results in complete inhibition of endogenous antibody production. Transfer of the human germ-line immunoglobulin gene array in such germ-line mutant mice will result in the production of human antibodies upon antigen challenge. See, e.g., 00 Jakobovits et al., Proc. Nail. Acad. Sci. USA, 90:2551 (1993); Jakobovits et al., Nature, 362:255-258 (1993); 0 Bruggermann et Year in Immuno., 7:33 (1993); and Duchosal et al. Nature 355:258 (1992).

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O 10 The monoclonal antibodies herein specifically include "chimeric" antibodies (immunoglobulins) in

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Swhich a portion of the heavy and/or light chain is identical with or homologous to corresponding sequences in O antibodies derived from a particular species or belonging to a particular antibody class or subclass, while the remainder of the chain(s) is identical with or homologous to corresponding sequences in antibodies derived from S another species or belonging to another antibody class or subclass, as well as fragments of such antibodies, so long as they exhibit the desired biological activity Patent No. 4,816,567; and Morrison et al., Proc. Natl Acad Sci. USA 81:6851-6855 (1984)).

The term "hypervariable region" when used herein refers to the amino acid residues of an antibody which are responsible for antigen-binding. The hypervariable region comprises amino acid residues from a "complementarity determining region" or "CDR" residues24-34 50-56 (L2) and 89-97 (L3) in the light chain variable domain and 31-35 (H 50-65 (H2) and 95-102 (H3) in the heavy chain variable domain; Kabat et al., Sequences ofPolypeptides of Immunological Interest, 5th Ed. Public Health Service, National Institutes of Health, Bethesda, MD. (1991)) and/or those residues from a "hypervariable loop" (ie. residues 26-32 (LI), 50-52 (L2) and 91-96 (L3) in the light chain variable domain and 26-32 (H 53-55 (H2) and 96-161 (H3) in the heavy chain variable domain; Chothia and Lesk J Mol. Biol. 196:901-917 (1987)). "Framework"or "FR" residues are those variable domain residues other than the hypervariable region residues as herein defined. The CDR and FR residues of the rhuMAb HER2 antibody of the example below (humAb4D5-8) are identified in Carter et al., Proc. Natl. Acad Sci. USA, 89:4285 (1992).

"Humanized" forms of non-human murine) antibodies are chimeric antibodies that contain minimal sequence derived from non-human immunoglobulin. For the most part, humanized antibodies are human immunoglobulins(recipient antibody) in which residues from a hypervariableregion of the recipient are replaced by residues from a hypervariable region of a non-human species (donor antibody) such as mouse, rat, rabbit or nonhuman primate having the desired specificity, affinity, and capacity. In some instances, Fv framework region (FR) residues of the human immunoglobulin are replaced by corresponding non-human residues. Furthermore, humanized antibodies may comprise residues which are not found in the recipient antibody or in the donor antibody. These modifications are made to further refine antibody performance. In general, the humanized antibody will comprise substantially all of at least one, and typically two, variable domains, in which all or substantially all of the hypervariable loops correspond to those of a non-human immunoglobulin and all or substantially all of the FR regions are those of a human immunoglobulin sequence. The humanized antibody

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PI241RI optionally also will comprise at least a portion of an immunoglobulin constant region typically that of a O human immunoglobulin.

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C, The choice of human variable domains, both light and heavy, to be used in making the humanized antibodies is very important to reduce antigenicity. According to the so-called "best-fit" method, the sequence

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J 5 of the variable domain of a rodent antibody is screened against the entire library of known human variabledomain sequences. The human sequence which is closest to that of the rodent is then accepted as the human framework (FR) for the humanized antibody (Sims et al, J Immunol., 151:2296 (1993); Chothia et J. Mol.

Biol., 196:901 (1987)).

O Another method uses a particular framework derived from the consensus sequence of all human 0 10 antibodies of a particular subgroup of light or heavy chains. The same framework may be used for several C, different humanized antibodies (Carter et at, Proc. Nail. Acad Sci. USA, 89:4285 (1992); Presta et al., J.

o Immnol., 151:2623 (1993)).

0 It is further important that antibodies be humanized with retention of high affinity for the antigen and other favorable biological properties. To achieve this goal, according to a preferred method, humanized antibodies are prepared by a process of analysis of the parental sequences and various conceptual humanized products using three-dimensional models of the parental and humanized sequences. Three-dimensional immunoglobulin models are commonly available and are familiar to those skilled in the art. Computer programs are available which illustrate and display probable three-dimensionalconformational structures of selected candidate immunoglobulin sequences. Inspection of these displays permits analysis of the likely role of the residues in the functioning of the candidate immunoglobulin sequence, the analysis of residues that influence the ability of the candidate immunoglobulin to bind its antigen. In this way, FR residues can be selected and combined from the recipient and import sequences so that the desired antibody characteristic, such as increased affinity for the target antigen(s), is achieved. In general, the CDR residues are directly and most substantially involved in influencing antigen binding.

"Antibody fragments" comprise a portion of a full length antibody, generally the antigen binding or variableregion thereof. Examples of antibody fragments include Fab, Fab', F(ab') 2 and Fv fragments; diabodies; Q linear antibodies; single-chain antibody molecules; and multispecific antibodies formed from antibody fragments.

Various techniques have been developed for the productionof antibody fragments. Traditionally, these fragments were derived via proteolytic digestion of intact antibodies (see, Morimoto et al, Journal ofBiochemicaland BiophysicalMethods 24:107-117(1992) and Brennan et al., Science, 229:81 (1985)). However, these fragments can now be produced directly by recombinant host cells. For example, the antibody fragments can be isolated from the antibody phage libraries discussed above. Alternatively, Fab'-SH fragments can be directly recovered from E. coli and chemically coupled to form F(ab') 2 fragments (Carter et al., Bio/Technology 10:163-167(1992)).

In another embodiment, the F(ab') 2 is formed using the leucine zipper GCN4 to promote assembly of the F(ab') 2 molecule. According to another approach, F(ab') 2 fragments can be isolated directly from recombinant host cell culture. Other techniques for the production of antibody fragments will be apparent to the skilled practitioner.

In other embodiments, the antibody of choice is a single chain Fv fragment (scFv). See WO 93/16185.

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P1241R1 "Single-chain Fv" or "sFv" antibody fragments comprise the VH and VL domains of antibody, wherein these

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O domains are present in a single polypeptide chain. Generally, the Fv polypeptide further comprises a polypeptide

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linker between the VH and VL domains which enables the sFv to form the desired structure for antigen binding.

T For a review of sFv see Pluckthun in The Pharmacology of Monoclonal Antibodies, vol. I13, Rosenburg and 5 Moore eds. Springer-Verlag, New York, pp. 269-315 (1994).

The term "diabodies" refers to small antibody fragments with two antigen-bindingsites, which fragments o0 comprise a heavy chain variable domain (VH) connected to a light chain variable domain (V

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in the same

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polypeptide chain (VI VL). By using a linker that is too short to allow pairing between the two domains on the

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0 same chain, the domains are forced to pair with the complementary domains of another chain and create two CMl 10 antigen-binding sites. Diabodies are described more fully in, for example, EP 404,097; WO 93/11161; and

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O Hollinger et al., Proc. NaIl. Acad. Sci. USA 90:6444-6448 (1993).

The expression "linear antibodies" when used throughout this application refers to the antibodies described in Zapata et al Polypeptide Eng. 8(10):1057-1062 (1995). Briefly, these antibodies comprise a pair of tandem Fd segments (VH-CI I -VH-CI i which form a pair of antigen binding regions. Linear antibodies can be bispecific or monospecific.

"Multispecific antibodies" have binding specificities for at least two different epitopes, where the epitopes are usually from different antigens. While such molecules normally will only bind two antigens (i.e.

bispecific antibodies, BsAbs), antibodies with additional specificities such as trispecific antibodies are encompassed by this expression when used herein. Examples of BsAbs include those with one arm directed against a tumor cell antigen and the other arm directed against a cytotoxic trigger molecule such as antianti-pl85HER2/FcyRIll (CD16), anti-CD3/anti-malignant B-cell (ID10), anti-CD3/antip1 85 HER2 anti-CD3/anti-p97, anti-CD3/anti-renal cell carcinoma, anti-CD3/anti-OVCAR-3, anti-CD3/L-Dl (anti-colon carcinoma), anti-CD3/anti-melanocytestimulating hormone analog, anti-EGF receptor/anti-CD3,anti- QO CD3/anti-CAMAl,anli-CD3/anti-CD9,anti-CD3/MoV 8,anti-neuralcell ahesion molecule (NCAM/anti-CD3, anti-folate binding protein (FBP)/anti-CD3,anti-pan carcinoma associated antigen (AMOC-3 i)/anti-CD3; BsAbs with one arm which binds specifically to a tumor antigen and one arm which binds to a toxin such as antisaporin/anti-ld- i, anti-CD22/anti-saporin, anti-CD7/anti-saporin, anti-CD38/anti-saporin, anti-CEA/anti-ricin A chain, anti-interferon-a(IFN-a)/anti-hybridoma idiotype, anti-CEA/anti-vinca alkaloid; BsAbs for converting enzyme activated prodrugs such as anti-CD30/anti-alkaline phosphatase (which catalyzes conversion of mitomycin phosphate prodrug to mitomycin alcohol); BsAbs which can be used as fibrinolytic agents such as anti-fibrin/anti-tissue plasminogen activator (tPA), anti-fibrin/anti-urokinase-type plasminogen activator (uPA); BsAbs for targeting immune complexes to cell surface receptors such as anti-low density lipoprotein (LDL)/anti- Fc receptor(e.g. FcyRl, or FcyRIII); BsAbs for use in therapy of infectiousdiseases such as anti-CD3/anti-herpes simplex virus (HSV), anti-T-cell receptor:CD3 complex/anti-influenza, anti-FcyR/anti-HIV; BsAbs for tumor HER2 detection in vitro or in vivo such as anti-CEA/anti-EOTUBE, anti-CEA/anti-DPTA, anti-p 185 /anti-hapten; BsAbs as vaccine adjuvants; and BsAbs as diagnostic tools such as anti-rabbit IgG/anti-ferritin, anti-horse radish

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P1241RI peroxidase (HRP)/anti-hormone, anti-somatostatin/anti-substance P, anti-HRP/anti-FITC, anti-CEA/anti-B- O galactosidase. Examples of trispecific antibodies include anti-CD3/anti-CD4/anti-CD37,anti-CD3/anti-CD5/anti-

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CD37 and anti-CD3/anti-CD8/anti-CD37. Bispecific antibodies can be prepared as full length antibodies or antibody fragments F(ab') 2 bispecific antibodies).

Methods for making bispecific antibodies are known in the art. Traditional production of full length Cl bispecific antibodies is based on the coexpression of two immunoglobulin heavy chain-light chain pairs, where the two chains have different specificities (Millstein et al., Nature, 305:537-539(1983)). Because of the random 00 assortment of immunoglobulin heavy and light chains, these hybridomas(quadromas) produce a potential mixture

O

of 10 different antibody molecules, of which only one has the correct bispecific structure. Purification of the

O

O 10 correct molecule, which is usually done by affinity chromatography steps, is rather cumbersome, and the product yields are low. Similar procedures are disclosed in WO 93/08829, and in Traunecker et al., EMBO 10:3655- 0 3659(1991).

Cl According to a different approach, antibody variable domains with the desired binding specificities S (antibody-antigen combining sites) are fused to immunoglobulin constant domain sequences. The fusion preferably is with an immunoglobulin heavy chain constant domain, comprising at least part of the hinge, CH2, and CH3 regions. It is preferred to have the first heavy-chain constant region (CHI) containing the site necessary for light chain binding, present in at least one of the fusions. DNAs encoding the immunoglobulin heavy chain fusions and, if desired, the immunoglobulin light chain, are inserted into separate expression vectors, and are cotransfected into a suitable host organism. This provides for great flexibility in adjusting the mutual proportions of the three polypeptide fragments in embodiments when unequal ratios of the three polypeptide chains used in the construction provide the optimum yields. It is, however, possible to insert the coding sequences for two or all three polypeptide chains in one expression vector when the expression of at least two polypeptide chains in equal ratios results in high yields or when the ratios are of no particular significance.

In a preferred embodiment of this approach, the bispecific antibodies are composed of a hybrid immunoglobulin heavy chain with a first binding specificity in one arm, and a hybrid immunoglobulin heavy chain-light chain pair (providing a second binding specificity) in the other arm. It was found that this asymmetric structure facilitates the separation of the desired bispecific compound from unwanted immunoglobulin chain combinations, as the presence of an immunoglobulin light chain in only one half of the bispecific molecule provides for a facile way of separation. This approach is disclosed in WO 94/04690. For further details of generating bispecific antibodies see, for example, Suresh et al., Methods in Enzymology, 121:210 (1986).

According to another approach described in WO96/2701 the interface between a pair of antibody molecules can be engineered to maximize the percentageof heterodimers which are recovered from recombinant cell culture. The preferred interface comprises at least a part of the CH3 domain of an antibody constant domain.

In this method, one or more small amino acid side chains from the interface of the first antibody molecule are replaced with larger side chains tyrosine or tryptophan). Compensatory "cavities" of identical or similar size to the large side chain(s) are created on the interface of the second antibody molecule by replacing large amino acid side chains with smaller ones alanine or threonine). This provides a mechanism for increasing the yield of the heterodimer over other unwanted end-products such as homodimers.

I

I

P1241R1 Bispecific antibodies include cross-linked or "heteroconjugate" antibodies. For example, one of the O antibodies in the heteroconjugatecan be coupled to avidin, the other to biotin. Such antibodies have, for example,

O

CN been proposed to target immune system cells to unwanted cells (US Patent No. 4,676,980), and for treatment of HIV infection (WO 91/00360, WO 92/200373, and EP 03089). Heteroconjugate antibodies may be made using any convenient cross-linking methods. Suitable cross-linking agents are well known in the art, and are disclosed Sin US Patent No. 4,676,980, along with a number of cross-linking techniques.

Techniques for generating bispecific antibodies from antibody fragments have also been described in 00 the literature. For example, bispecific antibodies can be prepared using chemical linkage. Brennan et al., Science, o 229: 81 (1985) describe a procedure wherein intact antibodies are proteolytically cleaved to generate F(ab') 2

O

O 10 fragments. These fragments are reduced in the presence of the dithiol complexing agent sodium arsenite to n stabilize vicinal dithiols and prevent intermolecular disulfide formation. The Fab' fragments generated are then

O

O converted to thionitrobenzoate (TNB) derivatives. One of the Fab'-TNB derivatives is then reconverted to the C" Fab'-thiol by reduction with mercaptoethylamine and is mixed with an equimolar amount of the other Fab'-TNB derivative to form the bispecific antibody. The bispecific antibodies produced can be used as agents for the selective immobilization of enzymes.

Recent progress has facilitated the direct recovery of Fab'-SH fragments from E. coli, which can be chemically coupled to form bispecific antibodies. Shalaby et J. Exp. Med., 175: 217-225 (1992) describe the production of a fully humanized bispecific antibody F(ab')2 molecule. Each Fab' fragment was separately secreted from E. coli and subjected to directed chemical coupling in vitro to form the bispecific antibody.

Various techniques for making and isolating bispecific antibody fragments directly from recombinant cell culture have also been described: For example, bispecific antibodies have been produced using leucine zippers. Kostelny et al., J Immunol., 148(5):1547-1553(1992). The leucine zipper peptides from the Fos and Jun proteins were linked to the Fab' portions of two different antibodies by gene fusion. The antibody homodimers were reduced at the hinge region to form monomers and then re-oxidized to form the antibody heterodimers.This method can also be utilized for the production of antibody homodimers. The "diabody" technology described by (D Hollinger et al. Proc. Nal. Acad. Sci. USA, 90:6444-6448 (1993) has provided an alternative mechanism for making bispecific antibody fragments. The fragments comprise a heavy-chain variable domain (VH) connected to a light-chain variable domain (VL) by a linker which is too short to allow pairing between the two domains on the same chain. Accordingly, the VH and VL domains of one fragment are forced to pair with the complementary VL and VH domains of another fragment, thereby forming two antigen-binding sites. Another strategy for making bispecific antibody fragments by the use of single-chain Fv (sFv) dimers has also been reported. See Gruber et al, J. Immunol, 152:5368 (1994).

Antibodies with more than two valencies are contemplated. For example, trispecific antibodies can be prepared. Tutt et al. J. Immunol. 147: 60 (1991).

The phrase "ion exchange material" refers to a solid phase which is negatively charged a cation exchange resin) or positively charged an anion exchange resin). The charge may be provided by attaching I P1241RI one or more charged ligands to the solid phase, e.g. by covalent linking. Alternatively, or in addition, the charge O may be an inherent property of the solid phase as is the case for silica, which has an overall negative charge).

O

Cl By "solid phase" is meant a non-aqueous matrix to which one or more charged ligands can adhere. The Ssolid phase may be a purification column, a discontinuous phase of discrete particles, a membrane, or filter etc.

Examples of materials for forming the solid phase include polysaccharides (such as agarose and cellulose); and other mechanically stable matrices such as silica controlled pore glass), poly(styrenedivinyl)benzene, polyacrylamide, ceramic particles and derivatives of any of the above.

SA "cation exchange resin" refers to a solid phase which is negatively charged, and which thus has free O cations for exchange with cations in an aqueous solution passed over or through the solid phase. A negatively O 10 charged ligand attached to the solid phase to form the cation exchange resin may, be a carboxylate or

O

Cl sulfonate. Commercially available cation exchange resins include carboxy-methyl-cellulose, BAKERBOND 0 ABX

TM

sulphopropyl(SP) immobilizedon agarose SP-SEPHAROSE FAST FLOW T M or SP-SEPHAROSE

O

C1 HIGH PERFORMANCE

T

from Pharmacia) and sulphonyl immobilized on agarose S-SEPHAROSE FAST

FLOW'

T from Pharmacia).

The term "anion exchange resin" is used herein to refer to a solid phase which is positively charged, e.g.

having one or more positively charged ligands, such as quaternary amino groups, attached thereto. Commercially available anion exchange resins include DEAE cellulose, QAE SEPHADEXTM and FAST Q SEPHAROSE

TM

(Pharmacia).

A "buffer" is a solution that resists chahges in pH by the action of its acid-base conjugate components.

Various buffers which can be employed depending, for example, on the desired pH of the buffer are described in Buffers. A Guidefor the Preparation and Use of Buffers in Biological Systems, Gueffroy, Ed. Calbiochem Corporation (1975). In one embodiment, the buffer has a pH in the range from about 5 to about 7 as in Example I below). Examples of buffers that will control the pH in this range include MES, MOPS, MOPSO, phosphate, acetate, citrate, succinate, and ammonium buffers, as well as combinations of these.

The "loading buffer" is that which is used to load the composition comprising the polypeptide molecule of interest and one or more contaminants onto the ion exchange resin. The loading buffer has a conductivity and/or pH such that the polypeptide molecule of interest (and generally one or more contaminants) is/are bound to the ion exchange resin.

The "intermediate buffer" is used to elute one or more contaminants from the ion exchange resin, prior to eluting the polypeptide molecule of interest. The conductivity and/or pH of the intermediate buffer is/are such that the contaminant is eluted from the ion exchange resin, but not significant amounts of the polypeptide of interest.

The term "wash buffer" when used herein refers to a buffer used to wash or re-equilibrate the ion exchange resin, prior to eluting the polypeptide molecule of interest. Conveniently, the wash buffer and loading buffer may be the same, but this is not required.

The "elution buffer" is used to elute the polypeptide of interest from the solid phase. The conductivity and/or pH of the elution buffer is/are such that the polypeptide of interest is eluted from the ion exchange resin.

A "regeneration buffer" may be used to regenerate the ion exchange resin such that it can be re-used. The regeneration buffer has a conductivity and/or pH as required to remove.substantially all contaminants and the

I

I

PI241R1 polypeptide of interest from the ion exchange resin.

O The term "conductivity" refers to the ability of an aqueous solution to conduct an electric current 0 Cl between two electrodes. In solution, the current flows by ion transport. Therefore, with an increasing amount of Sions present in the aqueous solution, the solution will have a higher conductivity. The unit of measurement for conductivity is mmhos (mS/cm), and can be measured using a conductivity meter sold, by Orion. The conductivity of a solution may be altered by changing the concentration of ions therein. For example, the concentration of a buffering agent and/or concentration of a salt NaC] or KC) in the solution may be altered 00 in order to achieve the desired conductivity. Preferably, the salt concentration of the various buffers is modified o to achieve the desired conductivity as in the Example below.

O 10 By "purifying" a polypeptide from a composition comprising the polypeptide and one or more Cl contaminants is meant increasing the degree of purity of the polypeptide in the composition by removing O (completely or partially) at least one contaminant from the composition. A "purification step" may be part of an C,1 overall purification process resulting in a "homogeneous" composition, which is used herein to refer to a composition comprising at least about 70% by weight of the polypeptide of interest, based on total weight of the composition, preferably at least about 80% by weight.

Unless indicated otherwise, the term "HER2" when used herein refers to human HER2 protein and "HER2" refers to human HER2 gene. The human HER2 gene and HER2 protein are described in Semba et aL., PNAS (USA) 82:6497-6501 (1985) and Yamamoto et al. Nature 319:230-234 (1986) (Genebank accession number X03363), for example.

The term "humMAb4D5-8" when used herein refers to a humanized anti-HER2 antibody comprising the light chain amino acid sequence of SEQ ID NO:] and the heavy chain amino acid sequence of SEQ ID NO:2 or amino acid sequence variants thereof which retain the ability to bind HER2 and inhibit growth of tumor cells which overexpress HER2 (see US Patent 5,677,171; expressly incorporated herein by reference).

The "pI" or "isoelectric point" of a polypeptide refer to the pH at which the polypeptide's positive charge balances its negative charge, pI can be calculated from the net charge of the amino acid residues of the polypeptide or can be determined by isoelectric focussing using CSx chromatography as in the Example 0 below).

By "binding" a molecule to an ion exchange material is meant exposing the molecule to the ion exchange material under appropriate conditions (pH/conductivity) such that the molecule is reversibly immobilized in or on the ion exchange material by virtue of ionic interactions between the molecule and a charged group or charged groups of the ion exchange material.

By "washing" the ion exchange material is meant passing an appropriate buffer through or over the ion exchange material.

To "elute" a molecule polypeptide or contaminant) from an ion exchange material is meant to remove the molecule therefrom by altering the ionic strength of the buffer surrounding the ion exchange material such that the buffer competes with the molecule for the charged sites on the ion exchange material.

"Treatment" refers to both therapeutic treatment and prophylactic or preventative measures. Those in need of treatment include those already with the disorder as well as those in which the disorder is to be prevented.

A "disorder" is any condition that would benefit from treatmentwith the polypeptide purified as described herein.

I

P1241R1 This includes chronic and acute disorders or diseases including those pathological conditions which predispose O the mammal to the disorder in question.

O

CN The word "label" when used herein refers to a detectable compound or composition which is conjugated Sdirectly or indirectly to the polypeptide. The label may be itself be detectable radioisotope labels or C 5 fluorescent labels) or, in the case of an enzymatic label, may catalyze chemical alteration of a substrate compound or composition which is detectable.

The term "cytotoxic agent" as used herein refers to a substance that inhibits or prevents the function of Scells and/or causes destruction of cells. The term is intended to include radioactive isotopes 131, 125, y 9 0

O

S and Re86), chemotherapeutic agents, and toxins such as enzymatically active toxins of bacterial, fungal, plant 0 10 or animal origin, or fragments thereof.

A "chemotherapeutic agent" is a chemical compound useful in the treatment of cancer. Examples of O chemotherapeutic agents include adriamycin, doxorubicin, epirubicin, 5-fluorouracil,cytosine arabinoside ("Ara-

TM

cyclophosphamide,-thiotepa, busulfan, cytoxin, taxoids, e.g. paclitaxel (TAXOL Bristol-Myers Squibb Oncology, Princeton, NJ), and doxetaxel, toxotere, methotrexate, cisplatin, melphalan, vinblastine, bleomycin, etoposide, ifosfamide, mitomycin C, mitoxantrone, vincristine, vinorelbine, carboplatin, teniposide, daunomycin, carminomycin, aminopterin, dactinomycin, mitomycins, esperamicins (see U.S. Pat. No. 4,675,187), melphalan and other related nitrogen mustards. Also included in this definition are hormonal agents that act to regulate or inhibit hormone action on tumors, such as tamoxifen and onapristone.

Modes for Carrying Out the Invention The invention herein provides a method for purifying a polypeptide from a composition an aqueous solution) comprising the polypeptide and one or more contaminants. The composition is generally one resulting from the recombinant production of the polypeptide,but may be that resulting from production of the polypeptide by peptide synthesis (or other synthetic means) or the polypeptide may be purified from a native source of the polypeptide. Preferably the polypeptide is an antibody, e.g. one which binds the HER2 antigen.

For recombinant production of the polypeptide, the nucleic acid encoding it is isolated and inserted into S a replicable vector for further cloning (amplification of the DNA) or for expression. DNA encoding the polypeptide is readily isolated and sequenced using conventional procedures where the polypeptide is an antibody by using oligonuclcotide probes that are capable of binding specifically to genes encoding the heavy and light chains of the antibody). Many vectors are available. The vector components generally include, but are not limited to, one or more of the following: a signal sequence, an origin of replication, one or more marker genes, an enhancer element, a promoter, and a transcription termination sequence as described in US Patent 5,534,615, specifically incorporated herein by reference).

Suitable host cells for cloning or expressing the DNA in the vectors herein are the prokaryote, yeast, or higher eukaryote cells described above. Suitable prokaryotes for this purpose include eubacteria, such as Gramnegative or Gram-positive organisms, for example, Enterobacteriaceae such as Escherichia, E. coli, Enterobacter, Erwinia, Klebsiella, Proteus, Salmonella, Salmonella typhimurium, Serratia, Serratia marcescans, and Shigella, as well as Bacilli such as B. subtilis and B. licheniformis B. licheniformis 41P disclosed in DD 266,710 published 12 April 1989), Pseudomonas such as P. aeruginosa, and Streptomyces. One

I

I

P1241RI preferred E. coli cloning host is E. coli 294 (ATCC 31,446), although other strains such as E. coli B, E. coli O X1776 (ATCC 31,537), and E. coli W3110 (ATCC 27,325) are suitable. These examples are illustrative rather

O

than limiting.

SIn addition to prokaryotes, eukaryotic microbes such as filamentous fungi or yeast are suitable cloning

C)

J 5 or expression hosts for polypeptide encoding vectors. Saccharomycescerevisiae, or common baker's yeast, is the most commonly used among lower eukaryotic host microorganisms. However, a number of other genera, species, and strains are commonly available and useful herein, such as Schizosaccharomycespombe; Kluyveromyces hosts such as, K. lactis, K. fragilis (ATCC 12,424), K bulgaricus (ATCC 6,045), K. wickeramii (ATCC 24,178), O K. waltii (ATCC 56,500), K. drosophilarum (ATCC 36,906), K. thermotolerans, and K. marxianus; yarrowia 0 10 (EP 402,226); Pichia pastoris (EP 183,070); Candida; Trichoderma reesia (EP 244,234); Neurospora crassa; Ci Schwanniomyces such as Schwanniomyces occidentalis; and filamentous fungi such as, Neurospora,

C)

o Penicillium, Tolypocladium, and Aspergillus hosts such as A. nidulans and A. niger.

SSuitable host cells for the expression of glycosylated polypeptide are derived from multicellular organisms. Examples of invertebrate cells include plant and insect cells. Numerous baculoviral strains and variants and corresponding permissive insect host cells from hosts such as Spodopterafrugiperda (caterpillar), Aedes aegypti (mosquito), Aedes albopictus (mosquito), Drosophila melanogaster (fruitfly), and Bombyx mori have been identified. A variety of viral strains for transfection are publicly available, the L-1 variant of Autographa californica NPV and the Bm-5 strain of Bombyx mori NPV, and such viruses may be used as the virus herein according to the present invention, particularly for transfection of Spodopterafrugiperda cells. Plant cell cultures of cotton, corn, potato, soybean, petunia, tomato, and tobacco can also be utilized as hosts.

However, interest has been greatest in vertebrate cells, and propagation of vertebrate cells in culture (tissue culture) has become a routine procedure. Examples of useful mammalian host cell lines are monkey kidney CVI line transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney line.(293 or 293 cells subcloned for growth in suspension culture, Graham et at., J. Gen Virol. 36:59(1977)); baby hamster kidney cells (BHK, ATCC CCL 10); Chinese hamster ovary cells/-DHFR (CHO, Urlaub et al., Proc. Natl. Acad. Sci. USA 77:4216 (1980)); mouse sertoli cells (TM4, Mather, Biol. Reprod. 23:243-251 (1980)); monkey kidney cells (CV I S ATCC CCL 70); African green monkey kidney cells (VERO-76, ATCC CRL- 1587); human cervical carcinoma cells (HELA, ATCC CCL canine kidney cells (MDCK, ATCC CCL 34); buffalo rat liver cells (BRL 3A, ATCC CRL 1442); human lung cells (W138, ATCC CCL 75); human liver cells (Hep G2, HB 8065); mouse mammary tumor (MMT 060562, ATCC CCL51); TRI cells (Mather et al., Annals N. Y. Acad Sci. 383:44-68 (1982)); MRC 5 cells; FS4 cells; and a human hepatoma line (Hep G2).

Host cells are transformed with the above-described expression or cloning vectors for polypeptide production and cultured in conventional nutrient media modified as appropriate for inducing promoters, selecting transformants, or amplifying the genes encoding the desired sequences.

The host cells used to produce the polypeptide of this invention may be cultured in a variety of media.

Commercially available media such as Ham's F10 (Sigma), Minimal Essential Medium (Sigma), RPMI- 1640 (Sigma), and Dulbecco's Modified Eagle's Medium ((DMEM), Sigma) are suitable for culturing the host cells. In addition, any of the media described in Ham et al., Meth. Enz. 58:44 (1979), Barnes et Anal.

Biochem.102:255 (1980), U.S. Pat. Nos. 4,767,704; 4,657,866; 4,927,762; 4,560,655; or 5,122,469; WO

I

P1241RI 90/03430; WO 87/00195; or U.S. Patent Re. 30,985 may be used as culture media for the host cells. Any of these O media may be supplementedas necessary with hormones and/or other growth factors (such as insulin, transferrin,

O

or epidermal growth factor), salts (such as sodium chloride, calcium, magnesium, and phosphate), buffers (such 0 as HEPES), nucleotides (such as adenosine and thymidine), antibiotics (such as GENTAMYCINTM drug), trace elements (defined as inorganic compounds usually present at final concentrations in the micromolar range), and glucose or an equivalent energy source. Any other necessary supplements may also be included at appropriate concentrations that would be known to those skilled in the art. The culture conditions, such as temperature, pH, Sand the like, are those previously used with the host cell selected for expression, and will be apparent to the S ordinarily skilled artisan.

O 10 When using recombinanttechniques, the polypeptidecan be produced intracellularly, in the periplasmic

O

space, or directly secreted into the medium. If the polypeptide is produced intracellularly, as a first step, the Ce o particulate debris, either host cells or lysed cells resulting from homogenization), is removed, for example,

O

C by centrifugation or ultrafiltration. Where the polypeptide is secreted into the medium, supernatants from such expression systems are generally first concentrated using a commercially available protein concentration filter, for example, an Amicon or Millipore Pellicon ultrafiltration unit.

The polypeptide is then subjected to one or more purification steps, including the ion exchange chromatography method as claimed herein. Examples of additional purification procedures which may be performed prior to, during, or following the ion exchange chromatography method include fractionation on a hydrophobic interaction chromatography on phenyl sepharose), ethanol precipitation, isoelectric focusing, Reverse Phase HPLC, chromatography on silica, chromatography on HEPARIN SEPHAROSE

M

further anion exchange chromatography and/or further cation exchange chromatography, chromatofocusing, SDS-PAGE, ammonium sulfate precipitation, hydroxylapatite chromatography, gel electrophoresis, dialysis, and affinity chromatography(e.g. using protein A, protein G, an antibody, a specific substrate, ligand or antigen as the capture reagent).

Ion exchange chromatography is performed as claimed herein. A decision is first made as to whether an anion or cation exchange resin is to be employed. In general, a cation exchange resin may be used for polypeptides with pl's greater than about 7 and an anion exchange resin may be used for polypeptides with pl's less than about 7.

The anion or cation exchange resin is prepared according to known methods. Usually, an equilibration buffer is passed through the ion exchange resin prior to loading the composition comprising the polypeptide and one or more contaminants onto the resin. Conveniently, the equilibration buffer is the same as the loading buffer, but this is not required.

The various buffers used for the chromatography depend, for example, on whether a cation or anion exchange resin is employed. This is shown more clearly in the flow diagrams of Figures 1 and 2.

With particular reference to Figure 1, which shows exemplary steps to be performed where a cation exchange resin is used, the pH and/or conductivity of each buffer is/are increased relative to the preceding buffer, except for the wash buffer where the conductivity and/or pH is/are less than the conductivity and/or pH of the preceding intermediate buffer. The aqueous solution comprising the polypeptide of interest and contaminant(s) is loaded onto the cation exchange resin using the loading buffer that is at a pH and/or conductivity such that the

I

P1241RI polypeptide and the contaminant bind to the cation exchange resin. As in the Example below, the loading buffer Smay be at a first low conductivity from about 5.2 to about 6.6 mmhos). An exemplary pH for the loading Cl buffer may be about 5.0 (see Fig. From about 20mg/mL to about 35mg/mL of the polypeptide of a full length antibody) may, for example, be loaded on the ion exchange resin.

The cation exchange resin is then washed with an intermediate buffer which is at a second conductivity C' and/or pH so as to essentially elute the contaminant, but not a substantial amount of the polypeptide of interest.

This may be achieved by increasing the conductivity or pH, or both, of the intermediate buffer. The change from OO loading buffer to intermediate buffer may be step-wise or gradual as desired. In the Example herein, the S intermediate buffer had a greater conductivity than that of the loading buffer the intermediate buffer's O 10 conductivity was in the range from about 7.3 to about 8.4 mmhos). Alternatively, as shown in Figure 1, the pH Cl of the intermediate buffer may exceed that of the loading buffer in this embodiment of the invention, where a O cation exchange resin is used. For example, the intermediate buffer may have a pH of about 5.4.

C Following washing with the intermediate buffer, the cation exchange resin is washed or re-equilibrated with the wash bufferwhich has a conductivity or pH, or both, which is/are less than that of the intermediate buffer the conductivity, or pH, or both, is/are changed in an opposite, i.e. reverse, direction to the preceding step, unlike ion exchange chromatography steps in the literature). In the Example below, the wash buffer had about the same conductivity as the loading buffer in the range from about 5.2 to about 6.6 mmhos) and its conductivity was, therefore, less than that of the intermediate buffer. In another embodiment, one may reduce the conductivity of the wash buffer to aconductivity that is less than, or greater than, that of the loading buffer, provided the conductivity of the wash buffer is less than that of the intermediate buffer. In another embodiment, the pH of the wash buffer may be less than the pH of the intermediate buffer the pH of the wash buffermay about The change in conductivity and/or pH of the wash buffer compared to the intermediate buffer may be achieved by step-wise or gradual change of either or both of these parameters.

After the wash step of the preceding paragraph, the cation exchange resin is prepared for elution of the desired polypeptide molecule therefrom. This is achieved using an elution buffer that has a pH and/or conductivity such that the desired polypeptideno longer binds to the cation exchange resin and therefore is eluted therefrom. The pH and/or conductivity of the elution buffer generally exceed(s) the pH and/or conductivity of the loading buffer, the intermediate buffer and the wash buffer used in the previous steps. In the Example below, the conductivity of the elution buffer was in the range from about 10.0 to about 11.0 mmhos. Alternatively, or in addition, the pH of the elution buffer may be increased relative to the wash buffer and to the intermediate buffer (for example, the pH of the elution buffer may about The change in conductivity and/or pH may be step-wise or gradual, as desired. Hence, the desired polypeptide is retrieved from the cation exchange resin at this stage in the method.

In an alternative embodiment, the ion exchange material comprises an anion exchange resin. This embodiment of the invention is depicted in Figure 2 herein. As illustrated in this figure, the changes in conductivity.are generally as described above with respect to a cation exchange resin. However, the direction of change in pH is different for an anion exchange resin. For example, if elution of the contaminant(s) and polypeptide are to be achieved by altering pH, the loading buffer has a first pH and the pH is decreased in the intermediate buffer so as to elute the contaminant or contaminants. In the third step, the column is washed/re-

I

I

P124IRI equilibrated with the wash buffer and the change in conductivity or pH, or both, is in the opposite direction to 0 that of the previous step. Hence, the pH may be increased in the wash buffer, compared to the intermediate buffer.

O

C Following this step, the polypeptide of interest is eluted from the anion exchange resin using an elution buffer 3 at a fourth conductivity and/or pH. If pH is altered, it will normally be less than the pH of the loading buffer, the J 5 intermediate buffer and the wash buffer. The change in pH and/or conductivity in progressive buffers can, as explained above, be step-wise or gradual.

In the preferred embodiment of the invention, a single parameter either conductivity or pH) is 00 changed to achieve elution of both the polypeptide and contaminant, while the other parameter pH or O conductivity, respectively) remains about constant. For example, while the conductivity of the various buffers O 10 (loading buffer, intermediate buffer, wash buffer and/or elution buffer) may differ, the pH's thereof may be (C essentially the same.

O In an optional embodiment of the invention, the ion exchange resin is regenerated with a regeneration o buffer after elution of the polypeptide, such that the column can be re-used. Generally, the conductivity and/or pH of the regeneration buffer is/are such that substantially all contaminants and the polypeptide of interest are eluted from the ion exchange resin. Generally, the regeneration buffer has a very high conductivity for eluting contaminants.and polypeptide from the ion exchange resin.

The method herein is particularly useful for resolving a polypeplide molecule of interest from at least one contaminant, where the contaminant and polypeptide molecule of interest differ only slightly in ionic charge.

For example, the pi's of the polypeptide and contaminant may be only "slightly different", for example they may differ.by only about 0.05 to about 0.2 pI units. In the Example below, this method could be used to resolve an anti-HER2 antibody having a pi of 8.87, from a singly-deamidated variant thereof having a pi of 8.79.

Alternatively, the method may be used to resolve a deamidated DNase, for example, from nondeamidated DNase.

In another embodiment, the method may be used to resolve a polypeptide from a glycosylation variant thereof, e.g. for resolving a variant of a polypeptide having a different distribution of sialic acid compared to the nonvariant polypeptide.

The polypeptide preparation obtained according to the ion exchange chromatography method herein may be subjected to additional purification steps, if necessary. Exemplary further purification steps have been discussed above.

Optionally, the polypeptide is conjugated to one or more heterologous molecules as desired. The heterologous molecule may, for example, be one which increases the serum half-life of the polypeptide (e.g.

polyethylene glycol, PEG), or it may be a label an enzyme, fluorescent label and/or radionuclide) or a cytotoxic molecule a toxin, chemotherapeutic drug, or radioactive isotope etc).

A therapeutic formulation comprising the polypeptide, optionally conjugated with a heterologous molecule, may be prepared by mixing the polypeptide having the desired degree of purity with optional pharmaceuticallyacceptable carriers, excipients or stabilizers (Remington's PharmaceuticalSciences 16th edition, Osol, A. Ed. (1980)), in the form of lyophilized formulations or aqueous solutions. "Pharmaceutically acceptable" carriers, excipients, or stabilizers are nontoxic to recipients at the dosages and concentrations employed, and include buffers such as phosphate, citrate, and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride; 18

I

P1241RI benzalkonium chloride, benzethonium chloride; phenol, butyl or benzyl alcohol; alkyl parabens such as methyl O or propyl paraben; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low molecularweight (less than

O

i about 10 residues) polypeptide; proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, histidine, arginine, J 5 or lysine; monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugars such as sucrose, mannitol, trehalose or sorbitol; salt- formingcounter-ions such as sodium; metal complexes Zn-protein complexes); and/or non-ionic surfactants such as TWEENTM, 00 PLURONICS TM or polyethylene glycol (PEG). The humMAb4D5-8 antibody of particular interest herein may O be prepared as a lyophilized formulation, e.g. as described in WO 97/04801; expressly incorporated herein by O 10 reference.

The formulationherein may also contain more than one active compound as necessary for the particular O indication being treated, preferably those with complementary activities that do not adversely affect each other.

o Such molecules are suitably present in combination in amounts that are effective for the purpose intended. For S example, for an anti-HER2 antibody a chemotherapeutic agent, such as a taxoid or tamoxifen, may be added to the formulation.

The active ingredients may also be entrapped in microcapsule prepared, for example, by coacervation techniques or by interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsule and poly-(methylmethacylate)microcapsule,respectively, in colloidal drug delivery systems (for example, liposomes albumin microspheres, microemulsions,nano-particlesand nanocapsules)or in macroemulsions.Such techniques are disclosed in Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed. (1980).

The formulation to be used for in vivo administration must be sterile. This is readily accomplished by filtration through sterile filtration membranes.

Sustained-release preparations may be prepared. Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing the polypeptide variant, which matrices are in the form of shaped articles, films, or microcapsule. Examples of sustained-release matrices include polyesters, hydrogels (for example, poly(2-hydroxyethyl-methacrylate), or poly(vinylalcohol)), polylactides Pat. No. 3,773,919), copolymers of L-glutamic acid andy ethyl-L-glutamate, non-degradable ethylene-vinyl acetate, degradable lactic acid-glycolic acid copolymers such as the LUPRON DEPOT

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(injectable microspheres composed of lactic acid-glycolic acid copolymer and leuprolide acetate), and poly-D-(-)- 3-hydroxybutyric acid.

The polypeptide purified as disclosed herein or the composition comprising the polypeptide and a pharmaceutically acceptable carrier is then used for various diagnostic, therapeutic or other uses known for such polypeptides and compositions. For example, the polypeptide may be used to treat a disorder in a mammal by administering a therapeutically effective amount of the polypeptide to the mammal.

The following examples are offered by way of illustration and not by way of limitation. The disclosures of all citations in the specification are expressly incorporated herein by reference.

EXAMPLE 1 Full length human IgG rhuMAb HER2 (humAb4D5-8 in Carter et al. Proc. Nail. Acad. Sci. 89: 4285- 4289 (1992) comprising the light chain amino acid sequence of SEQ ID NO:I and heavy chain amino acid

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P1241R1 sequence of SEQ ID NO:2) was produced recombinantly in CHO cells. Following protein production and 0 secretion to the cell culture medium, the CHO cells were separated from the cell culture medium by tangential 0 CI flow filtration (PROSTACK'). Protein A chromatography was then performed by applying the Harvested Cell 3 Culture Fluid (HCCF) from the CHO cells directly to an equilibrated PROSEP ATM column (Bioprocessing,Ltd).

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5 Following Protein A chromatography, cation exchange chromatography was performed using a sulphopropyi (SP)-SEPHAROSE FAST FLOW' T (SPSFF) column (Pharmacia) to further separate the desired anti-HER2 antibody molecule. The chromatography operation was performed in bind and elute mode.

The SPSFF column was prepared for load by sequential washes with regeneration buffer (0.025 M MES O 1.0 M NaCI, pH 5.6) followed by equilibration buffer (0.025 M MES 50 mM NaCI, pH The column was O 10 then loaded with Protein A pool adjusted to a pH of 5.60 0.05 and a conductivity of 5.8 0.2 mmhos. Prior to

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C elution, the column was washed in three steps: loading buffer (0.025 M MES 50 mM NaCI, pH 5.6) for a r O minimum of 1 column volume; intermediate buffer (0.025 M MES /70 mM NaCI, pH 5.6) until an apex of S a 280 nm peak was reached; and wash buffer (0.025 M MES 50 mM NaCI, pH 5.6) for a minimum of 1.2 column volumes. rhuMAb HER2 was then eluted from the column with elution buffer (0.025 M MES 95 mM NaCI, pH The elution 280 nm profile has a shoulder on the leading edge (Figure At the inflection point of this shoulder, pooling starts and continues for an additional 5 column volumes. The column was then regenerated with regeneration buffer (0.025 M MES 1-0 M NaCI, pH 5.6).

Materials and Methods Column andLoad Preparation: A reduced-scale SPSFF column was packed. The dimensions were: 27.0 mL volume, 1.0 cm diameter and 34.5 cm bed height. The pH of an aliquot of Protein A pool was titered to 5.6 with 1.5 M Tris base. The conductivity of the pool was reduced by the addition of an equal volume of sterile water for injection (SWFI).

Chromatography: The chromatography runs for this study were performed with Pharmacia's UNICORNTM FPLC system. The equilibration, load, and initial wash steps were performed at a linear flow rate of 200 cm/h. All chromatography steps were performed at a linear flow rate of 100 cm/h. The sequence of chromatography steps are defined in Table 1. A total of six chromatography runs were performed with load densities of 15, 20, 25, 30, 35, and 40 mg ofrhuMAb HER2 per mL of SPSFF resin.

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P1241R1 Table 1 Chromatography Steps' Chromatography Step Buffer Approximate Endpoint 2 Equilibration: Part 1 0.025 M MES 1.0 M NaCI, pH 5.6 2 CV Equilibration: Part 2 0.025 M MES 0.05 M NaCI, pH 5.6 pH: 5.6 0.1 Cond.: 5.8 0.2 mmhos Load Adjusted Protein A Pool As Required Wash 1 0.025 M MES 0.05 M NaCI, pH 5.6 1.5 CV Wash 2 0.025 M MES 0.07 M NaCI, pH 5.6 Apex of Peak Wash 3 0.025 M MES 0.05 M NaCl, pH 5.6 2 CV Elution: Prepool 0.025 M MES 0.095 M NaCI, pH 5.6 To Leading Shoulders Inflection Point CV) Elution: Pool 0.025 M MES 0.095 M NaCI, pH 5.6 5 CV Regeneration 0.025 M MES 1.0 M NaCI, pH 5.6 2 CV 1. The equilibration of the resin was performed in manual mode; the remaining steps were executed from a Pharmacia Unicorn Program.

2. CV column volume(s).

Total Protein: The protein concentration of each chromatography fraction (flow through, wash steps, elution prepool, elution pool, and regeneration) was determined by spectrophometric scans of each sample. The results were used to calculate product recovery yields. The extinction coefficient for rhuMAb HER2 is 1.45.

Calculations used to derive the results (Figure 4) are: 280 nm Protein Concentration (mg/mL) x Dilution Factor 1.45 Protein Mass (mg) in Each Fraction Protein Concentration (mg/mL) x Fraction Volume (mL) SFraction Mass (mg) Yield x 100 Total Mass (mg) Determination of rhuMAb HER2 Antibody Variants (CSx HPIEX): The rhuMAb HER2 SPSFF chromatography column resolves antibody variants. Fractions from each of the study chromatographies were tested for the relative amount of variant antibody by CSx HPIEX chromatography. A BAKERBOND WIDE- PORETM CSx HPIEX column (4.6 x 250mm) was run at 1 mL/min at 55 0 C. The mobile phase was formed from a tertiary gradient (Table 2).

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P1241RI Table 2 Gradient Scheme Time (min) A B C 0 Initial Conditions 49 1 10.0 40 10 50.0 33 17 50.2 49 1 70.0 49 1 The column is run at I mL/min at 55 0

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The A buffer was 0.025 M MES, pH 5.9; the B buffer was I M Ammonium Acetate, pH 7.0; and the C solution was sterile water for injection. The column was equilibrated with the gradient's initial conditions (49 A; 1% B; and 50% C) and 200 pl of sample, diluted with SWFI and containing< 300 pg protein, was injected.

Each resulting chromatogram was integrated to determine the percent area of each peak for each fraction (Table 3 and Figure Table 3 CSx HPIEX analysis of rhuMAb HER2 CSx Peak rhuMAb HER2 Variant a b Light Chain: Asn -*Asp deamidation and Other unidentifiable variation by tryptic map 1 Light Chain: Asn Asp deamidation 3 Fully Processed Antibody 102 4 Heavy Chain: Asp Iso-Asp and or -and/or- 450 Heavy Chain: An Additional Lys 102 Others Heavy Chain: Asp Succinimide and or Multiple permutations found in Peaks 1 and 4 Chromatograms Compared: The absorbance data (AU 280 nm) from each chromatography file was exported from Unicorn in ASCII format. The data from the 0.025 M MES 0.07 M NaCI, pH 5.6 wash was translated into Excel format and copied into KALEIDAGRAPHTM. Using KALEIDAGRAPHTM, the wash profiles were overlaid (Figure 6) and compared to each other.

RESULTS AND DISCUSSION- Deamidated and other acidic variants of rhuMAb HER2 were produced when the antibody was made 22

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P1241RI by recombinant DNA technology (see CSx peaks a, b and 1 in Figure The deamidated and other acidic

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O variants constituted about 25% (calculated as area under the integrated curve or profile obtained by CSx

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C, chromatography)of the composition obtained from the initial Protein A chromatographystep. It was discovered that the ion exchange method described herein could be used to substantially reduce the amount ofdeamidated

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C 5 and other acidic variants in the anti-HER2 composition, i.e. to about 13% or less the amount of acidic variants in the preparation subjected to cation exchange chromatography as described herein was decreased by about 50% or more).

O0 An absorbance trace from a cation exchange column run performed as described above is shown in O Figure 3. This method resolved a deamidated variant of anti-HER2 antibody that differed only slightly from O 10 nondeamidated anti-HER2 antibody. The increase in conductivity from the initial conditions to the intermediate Cl wash began to elute the deamidated anti-HER2 antibody. However, continued washing at this conductivity was O found to elute nondeamidated anti-HER2 antibody, resulting in a loss of product. Proceeding directly from the intermediate buffer to the elution buffer was observed to result in either an unacceptably low removal of deamidated anti-HER2 antibody from the product if pooling began early or unacceptably low yields of anti-HER2 antibody product if pooling was delayed until the deamidated anti-HER2 antibodywas reduced. It was discovered that by going back to lower conductivity as used initially, the elution of deamidated anti-HER2 antibody continued, without significant anti-HER2 antibody product elution.

The effect of rhuMAb HER2 load on buffer requirements, product recovery in the pool, and (c) product quality in the pool was evaluated.

At load densities of 15 mg/mL up to 35 mg/mL, the product yield in the elution pool is approximately For the load density of 40 mg/mL, the product yield in the pool dropped to 65% (Figure This reduced recovery in the pool is largely attributed to an increased antibody in the two wash steps (at 70 mM NaCI and mM NaCI, respectively).

The quality of rhuMAb HER2 in all the elution pools is equivalent as determined by CSx HPIEX analysis (Figure Compared to the load material; there is an enrichment of the nondeamidated antibody (Peak no change in the amount Iso-Asp 1 0 2 or Lys 45 antibody (Peak and a reduction of the amount of deamidated antibody (Peaks a, b, I and others).

The quality of rhuMAb HER2 in' these cation pools is improved through the intermediate wash step. As the mass of rhuMAb HER2 bound to the resin increases, the intermediate buffer volume consumption needed to reach the apex of the 280 nm peak decreases. The buffer volume required for a 40 mg/mL load density is approximately 2.5 column volumes. The buffer volume required for a 15 mg/mL load density is approximately column volumes. The exact increase of buffer requirement is not linear with the 5 mg/mL incremental changes between these two extremes. The greatest increase is seen between the load densities of 20 mg/mL and 15 mg/mL.

Here the requirement doubles from 7.5 column volumes to the previously mentioned 15 column volumes of buffer. If the apex of the 70 mM NaCI wash peak is reached, however, the product quality is equivalent for any of load densities examined.

This study determined how much rhuMAb HER2 can be loaded onto the SPSFF resin. Between the ranges of 15 to 40 mg of antibody per mL of resin, there is no difference in the quality of rhuMAb HER2

I

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P1241RI recovered in the elution pool- The quantity of rhuMAb HER2 recovered, however, is reduced by approximately O 10% when the resin is loaded with greater than 35 mg/mL. For consistent yields it is recommended that 35 mg/mL

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S be set as the maximum load for manufacture of rhuMAb HER2. Furthermore, due to the substantial increase in Q the 70 mM NaCI wash volume requirement between the 20 and 15 mg/mL; it is recommended that 20 mg/mL

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L 5 be set as the minimal load for manufacture ofrhuMAb HER2 Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or O group of integers or steps.

O The reference to any prior art in this specification is not, and should not be taken as, an acknowledgment or any form of suggestion that the prior art forms part of the common general knowledge in e Australia.

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Claims (1)

12-12-'06 13:27 FROM-DCC SYDNEY +61292621080 T-154 P008/010 F-139 M\WPDOCS\XhSflp''M Wfid6l lS4.d1Il*arvra O 0 Cl The claims defining the invention are as follows: 0) S1. A composition comprising a mixture of anti-HER2 antibody and one or more acidic Cl variants thereof, wherein the amount of the acidic variant(s) is less than about and wherein the acidic variant(s) are predominately deamidated variants wherein one 00 O or more asparagine residues of the anti-HER2 antibody have been deamidated, o and wherein the anti-HER2 antibody is humMAb4D5-8, Sand wherein the deamidated variants have Asn30 in CDR1 of either or both VL regions Cl Cn 10 of humMAb4D5-8 converted to aspartate. 0 C 2. The composition of claim 1, wherein the amount of the acidic variant(s) is less than about 3. The composition of claim 2, wherein the amount of the acidic variant(s) is less than about 13%. 4. The composition of claim 2, wherein the amount of the acidic variant(s) is in the range of about 1 to 18%. The composition of claim 1, wherein the anti-HER2 antibody comprises the light chain amino acid sequence of SEQ ID NO. 1 and the heavy chain amino acid sequence of SEQ ID NO. 2. 6. The composition of any one of claims 1 to 5, further comprising a pharmaceutically acceptable carrier. 7. A composition according to claim 1 substantially as hereinbefore described with reference to the examples and the accompanying figures. COMS ID No: SBMI-05641477 Received by IP Australia: Time 14:31 Date 2006-12-12