AU2002357406B2 - Defective entities and uses therefor - Google Patents

Description

WO 03/060105 PCT/AU02/01768 -1- DEFECTIVE ENTITIES AND USES THEREFOR FIELD OF THE INVENTION THE INVENTION relates generally to attenuated Bordetella strains of pathogenic origin. More particularly, the present invention relates to genetically modified Bordetella strains, which have been attenuated by disruption or inactivation of a gene encoding a metabolic protein, specifically a gene encoding a protein necessary for the biosynthesis of aromatic amino acids, and more specifically, the aroQ gene that encodes a dehydroquinase enzyme. The genetically modified Bordetella strain of the present invention has a reduced capacity to propagate in a mammalian host, but remains viable in the host for a period of time sufficient to induce a protective immune response against the natural pathogenic Bordetella counterpart. The present invention, therefore, also relates to the use of such genetically modified Bordetella strains in immunopotentiating compositions for treating and/or preventing inter alia Bordetella infections, and particularly pathogenic infections, caused by Bordetella. The present invention is also directed to the structure and sequence of aroQ from Bordetella pertussis, which are useful inter alia for the production of the genetically modified attenuated Bordetella strains of the present invention and for detecting and isolating variant aroQ genes and expression products.

Bibliographic details of various publications referred to by author in this specification are collected at the end of the description.

BACKGROUND OF THE INVENTION Whooping cough (pertussis), a respiratory disease caused by Bordetella pertussis, accounts for more than 300,000 deaths annually worldwide (Galazka 1992). B. pertussis is a non-invasive pathogen which localises to the tracheobronchial tree and produces a large array of potential virulence factors, which may play a role in the pathogenesis of pertussis (Weiss and Hewlett, 1986). A killed whole cell pertussis vaccine, generally given in combination with diphtheria and tetanus toxoids, has been available in many countries for over 40 years, and while its use seems to control pertussis epidemics, concerns over the reactogenicity, ranging from high fever, persistent crying, pain at the site of injection and possible existence of acute encephalopathy (Baraff et al. 1984; Pollock et al. 1984; Howson Fineberg 1992) led to the development of acellular pertussis vaccines. Children under the age of two and up to the age of five years are extremely susceptible to whooping WO 03/060105 PCT/AU02/01768 -2cough, however, the recent concern that young adults (vaccinated during their childhood) with waning immunity against whooping may serve as a reservoir for the pathogen, and thus may be passing it on to their children, have stimulated interest in the development of an alternative vaccine which can also be used safely in the adult population. The protective efficacy of the acellular vaccine has been the subject of controversy, however. Apart from the controversy on the efficacy of the acellular vaccine (DTaP), recent reports have highlighted that serious side reactions, particularly extensive swelling of the injected limb, occur in a notable percentage of children receiving booster vaccinations with DTaP (Rennels et al., 2000). The alternatives suggested by Mills (2001) are either to reduce the number of booster shots with ensuing reduced levels of immunity or to find a replacement adjuvant, which, unlike alum, favours the induction of Thl responses that have been shown to be responsible for long-term protection against whooping cough.

Although the exact nature of immunity against infection with B. pertussis is still not completely understood, animal experiments are providing increasing evidence that while circulating antibodies may play a role in toxin neutralisation and prevention of bacterial attachment to respiratory epithelial cells, other immunological mechanisms such as cell-mediated immunity (CMI) may also be necessary for complete long-term protection (Mills et al. 1993). This suggestion is supported by the observation that B. pertussis is not exclusively an extracellular pathogen and can be internalised and survive within mammalian cells including macrophages (Ewanowich et al. 1989; Saukkonen et al. 1991).

These findings suggest that protection against facultative intracellular pathogens, such as B. pertussis, may depend upon acquired cell-mediated resistance and activation of macrophages by interferon (IFN)-y-producing T lymphocytes. Another cytokine, interleukin 12 (IL-12), a heterodimeric cytokine produced by monocyte/macrophages and B cells, which is known to increase activity of natural killer (NK) cells, has also been reported (Manetti et al. 1993) to shift the cytokine pattern of the CD4 cell types from a Th2 (IL4, IL5) to a Thl cytokine secretion pattern (IFN-y and IL-2).

Redhead et al. (1993) reported that mice either convalescing from an active infection or those immunised with the inactivated whole cell vaccine eliminated bacteria significantly more effectively than those receiving an acellular vaccine. This finding was attributed to the induction of Thl-mediated cellular immune response by the inactivated whole cell vaccine and Th2-mediated humoral immune response by the acellular vaccine.

WO 03/060105 PCT/AU02/01768 -3- These findings were contradicted by Zepp et al. (1996) who reported the induction of both the humoral and Thl-lymphocyte-mediated cytokine profiles.

More recently Canthaboo et al. (2000) compared the humoral and cellular immune responses of mice following vaccination with the killed whole cell pertussis vaccine (WCV: DTPw) and with DTaP and reported that the DTPw, although inducing a lower antibody titres to the pertussis toxin, filamentous haemagglutinin and pertactin, was more effective in activating macrophages and more protective as judged in intracerebral challenge and bacterial lung clearance experiments than the DTaP. These authors suggested that cell-mediated immunity may play a crucial role in eliminating bacteria which escape humoral defence mechanisms.

In the development of vaccines against many intracellular pathogens such as Salmonella species and Shigella species, live attenuated vaccines such as aroA-inactivated S. typhimurium and aroD-inactivated Shigella flexneri have been developed and found to stimulate both antibody and cell-mediated immune responses, which are protective in mouse models and the target species (Mukkur et al. 1987; Verma Lindberg 1991).

However, the inactivation of a particular gene in one pathogen that leads to an attenuated phenotype with effective stimulation of antibody and cell-mediated immune responses is generally not extrapolatable to other pathogens. For example, Roberts et al. (1990) developed an aromatic-dependent mutant (aroA) of B. pertussis, but it was found to persist in the lungs of mice for only a short period of time (4 days at reasonable numbers) thus casting doubt on its ability to stimulate the cell-mediated immunity (CMI) required for long-lasting protection. This result was unexpected given previous reports regarding the success of the aroA mutant of Salmonella species (Hoiseth and Stocker, 1981; Mukkur et al. 1987) as a successful vaccine. On the other hand, the aroA mutants of Shigella species were found to be poorer vaccines than the aroD deletion mutants of the same species (Verma and Lindberg 1991).

To date, it has not been possible to inactivate any specific gene of a Bordetella strain to permit the production of defective strain that is able to replicate only at a very low level in the host and that persists in the host long enough to allow the induction of a hostprotective immune response, particularly cell-mediated immunity.

WO 03/060105 PCT/AU02/01768 -4- SUMMARY OF THE INVENTION In work leading up to the present invention, the inventors determined that B. pertussis contains a gene with amino acid sequence homology to the aroQ-encoded 3dehydroquinase enzyme of Actinobacillus pleuropneumoniae, which has been reported previously to have homology with the eukaryotic genes in the quinic acid catabolic pathway of Aspergillus nidulans. Even though the sequence of this gene indicated its involvement in the catabolic pathway, the inventors discovered that it rescued an E. coli aroD mutant and that it, therefore, was capable of functioning in the aromatic amino acid biosynthetic pathway ofB. pertussis. Further investigation also confirmed that B. pertussis did not contain an aroD gene, supporting the concept that there was only one gene encoding a 3-dehydroquinase in B. pertussis. It was also discovered that inactivation of the aroQ gene is effective in attenuating B. pertussis whilst retaining its capacity to stimulate both an antibody immune response and a cell-mediated immune response. This finding is surprising in the light of a previous report showing that deletion of aroQ in Corynebacterium pseudotuberculosis results in over-attenuation (Simmons et al. 1998), thereby making the recombinant strain unsuitable as a vaccine against the disease syndrome caused by the parent strain, caseous lymphadenitis (CLA) in sheep. By contrast, the aroQ mutant of B. pertussis is not too highly attenuated and hence is more attractive as a vaccine candidate against whooping cough. In accordance with the present invention, it is predicted that the inactivation of aroQ in other pathogenic strains of Bordetella would also confer the same properties. Thus, the present invention provides for the first time genetically modified Bordetella strains of pathogenic origin that have been attenuated by disruption or inactivation of a gene encoding a metabolic protein, particularly a gene encoding a protein necessary for the biosynthesis of aromatic amino acids, and more particularly, the aroQ gene that codes for a 3-dehydroquinase enzyme.

Accordingly, in one aspect of the present invention, there is provided a genetically modified Bordetella strain having a partial or complete loss of function in the endogenous aroQ gene and a lower capacity to propagate in a mammalian host but remaining viable in the host for a period of time sufficient to induce an immune response against a pathogenic Bordetella strain, preferably a natural pathogenic Bordetella counterpart of the genetically modified Bordetella strain, or related organism. In a preferred embodiment, the genetically modified strain comprises a disruption in the endogenous aroQ gene. Suitably, the disruption has been introduced into the genome of a pathogenic strain of Bordetella by WO 03/060105 PCT/AU02/01768 homologous recombination with a DNA targeting construct such that the targeting construct is stably integrated in that genome, wherein the disruption of the aroQ gene results in an inability of the genetically modified Bordetella strain to produce a functional 3-dehydroquinase or detectable levels of the dehydroquinase. In an alternate embodiment, the genetically modified strain comprises an exogenous nucleic acid sequence in its genome, or on an extrachromosomal element such as a plasmid, which is capable of abolishing or otherwise reducing the expression of aroQ or the level and/or functional activity of the 3-dehydroquinase encoded by aroQ. Suitably, the nucleic acid sequence comprises at least a portion of aroQ, in the sense or anti-sense orientation, which is operably linked to a transcriptional control element. Alternatively, the nucleic acid sequence comprises a ribozyme-encoding polynucleotide that is operably linked to a transcriptional control element, wherein the ribozyme specifically binds to or otherwise interacts with a transcript of the aroQ gene.

In a related aspect, the present invention contemplates a genetically modified Bordetella strain as broadly described above comprising at least one exogenous gene which is capable of expressing an antigen that is heterologous or foreign to the Bordetella strain. This embodiment is particularly useful for the design of immunopotentiating compositions against unrelated pathogens. Accordingly, the heterologous or foreign antigen is preferably derived from a pathogen that is unrelated to the Bordetella strain and, in a preferred embodiment, the pathogen is one that infects by the mucosal route.

As will be apparent from the foregoing, the structure and sequence of aroQ from Bordetella pertussis is useful for the production of the genetically modified attenuated Bordetella strains of the present invention. Accordingly, in another aspect of the present invention there is provided an isolated polynucleotide comprising a nucleotide sequence that corresponds or is complementary to at least a portion of the sequence set forth in SEQ ID NO: 1. In a preferred embodiment, the polynucleotide comprises the sequence set forth in SEQ ID NO: 3.

In one embodiment, the nucleotide sequence is a variant having at least preferably at least 60%, more preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, and still even more preferably at least 95%, 96%, 97%, 98% or 99% sequence identity to at least a portion of the sequence set forth in SEQ ID NO: 1 or 3. In another embodiment, the variant is capable of hybridising to at least a portion of the sequence set forth in SEQ ID NO: 1 or 3 under at least low stringency conditions, WO 03/060105 PCT/AU02/01768 -6preferably under at least medium stringency conditions, and more preferably under high stringency conditions.

Suitably, the portion is at least 18 nucleotides, preferably at least 25 nucleotides, more preferably at least 50 nucleotides, even more preferably at least 100 nucleotides, even more preferably at least 150 nucleotides, even more preferably at least 200 nucleotides, even more preferably at least 300 nucleotides, even more preferably at least 400 nucleotides, and still even more preferably at least 500 nucleotides in length. Preferably, the portion is a biologically active fragment of the sequence set forth in SEQ ID NO: 1 or 3.

The 3-dehydroquinase enzyme encoded by the aroQ gene of the present invention is useful inter alia in the design of immunopotentiating compositions or for the production of antigen-binding molecule that are interactive with that enzyme, which could be utilised in screening for aroQ- mutants of Bordetella or for detecting or isolating variant 3dehydroquinase enzymes. Thus, in yet another aspect of the present invention there is provided an isolated polypeptide comprising an amino acid sequence that corresponds to at least a portion of the sequence set forth in SEQ ID NO: 2.

Suitably, the amino acid sequence is a variant having at least 50%, preferably at least 60%, more preferably at least 70%, even more preferably at least 80%, even more preferably at least 90%, and still even more preferably at least 95%, 96%, 97%, 98% or 99% sequence identity to at least a portion of the sequence set forth in SEQ ID NO: 2.

Suitably, the portion is at least 6 amino acids, preferably at least 10 amino acids, more preferably at least 20 amino acids, even more preferably at least 30 amino acids, even more preferably at least 50 amino acids, even more preferably at least 70 amino acids, even more preferably at least 100 amino acids and still even more preferably at least 130 amino acids in length. Preferably the portion is a biologically active fragment of the sequence set forth in SEQ ID NO: 2.

Another aspect of the present invention pertains to a nucleic acid construct for disrupting an aroQ gene in a Bordetella cell, comprising: a) a non-homologous replacement portion; b) a first homology region located upstream of the non-homologous replacement portion, the first homology region having a nucleotide sequence with substantial identity to a first aroQ gene sequence; and c) a second homology region located downstream of the non-homologous replacement portion, the second homology region having a nucleotide sequence with substantial identity to a second aroQ gene sequence, the WO 03/060105 PCT/AU02/01768 -7second aroQ gene sequence having a location downstream of the first aroQ gene sequence in a naturally occurring endogenous aroQ gene of the Bordetella cell. Additionally, the first and second homology regions are of sufficient length for homologous recombination to occur between the nucleic acid construct and the endogenous aroQ gene when the nucleic acid molecule is introduced into the Bordetella cell. In a preferred embodiment, the aroQ gene comprises the sequence set forth in SEQ ID NO: 1 or 3 or variant or derivative thereof In another aspect, the invention contemplates a vector comprising a nucleotide sequence that corresponds or is complementary to at least a portion of the sequence set forth in SEQ ID NO: 1 or 3, or a nucleic acid construct as broadly described above. In a preferred embodiment, the vector is a DNA targeting vector.

In yet another aspect, the invention envisions a host cell containing a vector as broadly described above.

In another aspect, the invention embraces an antigen-binding molecule that is specifically interactive with the polypeptide, portion, variant or derivative according to the present invention.

The present invention further provides a method for producing a genetically modified Bordetella strain, comprising: introducing the nucleic acid construct as broadly described above into a Bordetella cell under conditions such that the nucleic acid construct is homologously recombined into the aroQ gene in the genome of that cell to produce a genetically modified Bordetella cell containing a disrupted aroQ gene.

In a preferred embodiment, the genetically modified Bordetella cell containing the homologously recombined nucleic acid construct is further characterised by expressing reduced or undetectable levels of aroQ. In another preferred embodiment, the genetically modified Bordetella cell lacks the ability to produce a functional 3-dehydroquinase encoded by said aroQ gene.

The genetically modified Bordetella strain of the present invention is useful for the design of immunopotentiating compositions that are effective in eliciting an immune response, and preferably a protective immune response, against a pathogenic Bordetella strain, preferably a natural pathogenic Bordetella counterpart of the genetically modified Bordetella strain, or related organism. Thus, in yet another aspect, the invention contemplates a composition, comprising a genetically modified Bordetella strain as WO 03/060105 PCT/AU02/01768 -8broadly described above, together with a pharmaceutically acceptable carrier. The composition may optionally comprise an adjuvant.

In a related aspect, the invention encompasses a composition of matter comprising dendritic cells which have been exposed to a genetically modified Bordetella strain as broadly described above for a time and under conditions sufficient to express a processed or modified antigen derived from the Bordetella strain for presentation to, and modulation of, T cells. This embodiment is particularly useful for the design of immunopotentiating compositions for eliciting a humoral and a cell mediated immune response. In one embodiment, the composition is in the form of an in vitro cell culture.

In a further aspect, the invention encompasses a method for modulating an immune response, which response is preferably against a pathogenic strain of Bordetella or related organism, comprising administering to a patient in need of such treatment an effective amount of a genetically modified Bordetella strain, or a composition, as broadly described above.

According to still a further aspect of the present invention, there is provided a method for the treatment and/or prophylaxis of whooping cough or related condition, comprising administering to a patient in need of such treatment an effective amount of a genetically modified Bordetella strain, or a composition, as broadly described above.

The invention also encompasses the use of a genetically modified Bordetella strain as broadly described above in the study, and modulation of an immune response, which is preferably against a pathogenic strain of Bordetella or related organism.

WO 03/060105 PCT/AU02/01768 -9- BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a diagrammatic representation showing a plasmid map of pUSQBord4, which restored aroD mutant, E. coli 583/90, to wild type.

Figure 2 is a diagrammatic representation showing a Pileup alignment of the aroQ gene sequence present in the 1 kb fragment of B. pertussis and other published aroQ sequences.

Figure 3 is a schematic representation illustrating the construction of shuttle vector pUSQBordl0.

Figure 4 is a photographic representation of an agarose gel showing the results of a polymerase chain reaction (PCR) amplification of genomic DNA (gDNA) from a B.

pertussis mutant that fails to grow in the absence of aromatic amino acid supplements. The results confirm the disruption of the aroQ gene by the kanamycin resistance cassette of pUSQBordlO. Lanes are as follows: Lane 1, HindUII cut k Standard (Gibco BRL); Lane 2, Blank; Lane 3, B. pertussis aroQ mutant gDNA; Lane 4, B. pertussis BP304; Lane 5, E.

coli gDNA; and Lane 6, Low DNA Mass Ladder (Gibco BRL).

Figure 5A is a graphical representation showing the persistence of B. pertussis aroQ mutant in the lungs of mice at 1, 3, 5, 7, 14 and 21 days post inoculation (no microorganisms were isolated at day 21, resulting in the exclusion of that data point from the graph).

Figure 5B is a graphical representation showing the persistence of B. pertussis aroQ mutant in the lungs of mice at 2, 4, 6, 7, 8, 9, 10 11 and 12 days post inoculation.

Figure 5C is a graphical representation showing the persistence of B. pertussis Tahoma I in the lungs of mice following inoculation.

Figure 6 is a graphical representation showing clearance of infection in lungs of vaccinated and control mice after intranasal challenge with a 2.0x 108 CFU dose of virulent B. pertussis Tohama I.

Figure 7A is a graphical representation showing production of IL-2 by T-cells in pooled mouse spleen samples stimulated in vitro with PTxoid.

Figure 7B is a graphical representation showing production of IL-2 by T-cells in pooled mouse spleen samples stimulated in vitro with FHA.

WO 03/060105 PCT/AU02/01768 Figure 8A is a graphical representation showing production of IFN-y by T-cells in pooled mouse supernatants from spleen cells stimulated in vitro with PTxoid.

Figure 8B is a graphical representation showing production of IFN-y by T-cells in pooled mouse supernatants from spleen cells stimulated in vitro with FHA.

WO 03/060105 WO 03/60105PCT/AU02IO1 768 I1I BRIEF DESCRIPTION OF THE SEQUENCES: SUMMARY TABLE TABLE A SEQUENCE ID SEQUENCE LENGTH

NUMBER

SEQ LD NO: I B. pertussis aroQ gene 942 nts SEQ ID NO: 2 3-Dehydroquinase encoded by SEQ 11D NO: 1 144 aa SEQ liD NO: 3 B. pertussis aroQ coding sequence 435 nts SEQ LD NO: 4 FORKAN primer SEQ ID NO: 5 BACKAN primer 30 nts SEQ ID NO: 6 FORQ primer 20 nts SEQ ID NO: 7 BACQ2 primer 20 nts WO 03/060105 PCT/AU02/01768 -12- DETAILED DESCRIPTION OF THE INVENTION 1. Definitions Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the invention belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, preferred methods and materials are described. For the purposes of the present invention, the following terms are defined below.

The articles "a and "an are used herein to refer to one or to more than one (i.e.

to at least one) of the grammatical object of the article. By way of example, "an element" means one element or more than one element.

The term "about" is used herein to refer to the position or location of a subunit in a polymer the location of a particular nucleotide in a nucleic acid sequence) that varies by as much as 10 subunits, preferably by as much 5 subunits, and more preferably by as much as 2 subunits from a specified position or location. Alternatively, the term "about" is used herein to refer to conditions amounts, concentrations, time etc) that vary by as much as 30%, preferably by as much as 20%, and more preferably by as much as 10% to a specified condition.

"Amplification product" refers to a nucleic acid product generated by nucleic acid amplification techniques.

By "antigen-binding molecule is meant a molecule that has binding affinity for a target antigen. It will be understood that this term extends to immunoglobulins, immunoglobulin fragments and non-immunoglobulin derived protein frameworks that exhibit antigen-binding activity.

"Antigenic or immunogenic activity" refers to the ability of a polypeptide, fragment, variant or derivative according to the invention to produce an antigenic or immunogenic response in an animal, preferably a mammal, to which it is administered, wherein the response includes the production of elements which specifically bind to the polypeptide or fragment thereof.

By "biologically active fragment" is meant a fragment of a full-length parent polypeptide which fragment retains the activity of the parent polypeptide 3- WO 03/060105 PCT/AU02/01768 13dehyfroquinase activity or antigenic or immunogenic activity). As used herein, the term "biologically active fragment" includes deletion variants and small peptides, for example of at least 6, preferably at least 10, more preferably at least 20 and even more preferably at least 30 contiguous amino acids, which comprise the above activity. Peptides of this type may be obtained through the application of standard recombinant nucleic acid techniques or synthesised using conventional liquid or solid phase synthesis techniques. For example, reference may be made to solution synthesis or solid phase synthesis as described, for example, in Chapter 9 entitled "Peptide Synthesis" by Atherton and Shephard which is included in a publication entitled "Synthetic Vaccines" edited by Nicholson and published by Blackwell Scientific Publications. Alternatively, peptides can be produced by digestion of a polypeptide of the invention with proteinases such as endoLys-C, endoArg-C, endoGlu-C and staphylococcus V8-protease. The digested fragments can be purified by, for example, high performance liquid chromatographic (HPLC) techniques.

The term "complementary" refers to the topological capability or matching together of interacting surfaces of a test polynucleotide and its target oligonucleotide, which may be part of a larger polynucleotide. Thus, the test and target polynucleotides can be described as complementary, and furthermore, the contact surface characteristics are complementary to each other. Complementary includes base complementarity such as A is complementary to T or U, and C is complementary to G in the genetic code. However, this invention also encompasses situations in which there is non-traditional base-pairing such as Hoogsteen base pairing which has been identified in certain transfer RNA molecules and postulated to exist in a triple helix. In the context of the definition of the term "complementary", the terms "match" and "mismatch" as used herein refer to the hybridisation potential of paired nucleotides in complementary nucleic acid strands.

Matched nucleotides hybridise efficiently, such as the classical A-T and G-C base pair mentioned above. Mismatches are other combinations of nucleotides that hybridise less efficiently.

Throughout this specification, unless the context requires otherwise, the words "comprise "comprises and "comprising" will be understood to imply the inclusion of a stated step or element or group of steps or elements but not the exclusion of any other step or element or group of steps or elements.

By "corresponds to" or "corresponding to" is meant a polynucleotide having a nucleotide sequence that is substantially identical or complementary to all or a portion of a WO 03/060105 PCT/AU02/01768 -14reference polynucleotide sequence or encoding an amino acid sequence identical to an amino acid sequence in a peptide or protein; or a peptide or polypeptide having an amino acid sequence that is substantially identical to a sequence of amino acids in a reference peptide or protein.

By "derivative" is meant a polypeptide that has been derived from the basic sequence by modification, for example by conjugation or complexing with other chemical moieties or by post-translational modification techniques as would be understood in the art.

The term "derivative" also includes within its scope alterations that have been made to a parent sequence including additions or deletions that provide for functional equivalent molecules.

The term "foreign" or "exogenous" or "heterologous" refers to any molecule a polynucleotide or polypeptide) which is introduced into a host by experimental manipulations and may include gene sequences found in that host so long as the introduced gene contains some modification a point mutation, the presence of a selectable marker gene, the presence of a recombination site, etc.) relative to the naturally-occurring gene.

The term "gene" as used herein refers to any and all discrete coding regions of the cell's genome, as well as associated non-coding and regulatory regions. Thus, the term "aroQ gene" is used generically herein to designate aroQ genes, e.g. variants from different Bordetella species. The gene is also intended to mean the open reading frame encoding specific polypeptides and adjacent 5' and 3' non-coding nucleotide sequences involved in the regulation of expression, up to about 1 kb beyond the coding region, but possibly further in either direction. In this regard, the gene may further comprise endogenous naturally associated with a given gene) or heterologous control signals such as promoters, enhancers, translational control elements such a Shine-Dalgarno sequence, initiation codon, termination codons and/or transcriptional termination signals.

The gene may be introduced into an appropriate vector for extrachromosomal maintenance or for integration into the host.

By "effective amount", in the context of modulating an activity or of treating or preventing a condition is meant the administration of that amount of active to an individual in need of such modulation, treatment or prophylaxis, either in a single dose or as part of a series, that is effective for modulation of that effect or for treatment or prophylaxis of that condition or for ameliorating the symptoms associated with that condition. The effective WO 03/060105 PCT/AU02/01768 amount will vary depending upon the health and physical condition of the individual to be treated, the taxonomic group of individual to be treated, the formulation of the composition, the assessment of the medical situation, and other relevant factors. It is expected that the amount will fall in a relatively broad range that can be determined through routine trials.

"Homology" refers to the percentage number of amino acids that are identical or constitute conservative substitutions as defined in Table B below. Homology may be determined using sequence comparison programs such as GAP (Deveraux et 1984, Nucleic Acids Research 12, 387-395) which is incorporated herein by reference. In this way sequences of a similar or substantially different length to those cited herein could be compared by insertion of gaps into the alignment, such gaps being determined, for example, by the comparison algorithm used by GAP.

"Hybridisation" is used herein to denote the pairing of complementary nucleotide sequences to produce a DNA-DNA hybrid or a DNA-RNA hybrid. Complementary base sequences are those sequences that are related by the base-pairing rules. In DNA, A pairs with T and C pairs with G. In RNA U pairs with A and C pairs with G. In this regard, the terms "match" and "mismatch" as used herein refer to the hybridisation potential of paired nucleotides in complementary nucleic acid strands. Matched nucleotides hybridise efficiently, such as the classical A-T and G-C base pair mentioned above. Mismatches are other combinations ofnucleotides that do not hybridise efficiently.

Reference herein to "interactive" includes reference to any interaction, reaction, or other form of association between molecules and in particular where one of the molecules is, or mimics, a component of the immune system.

By "isolated" is meant material that is substantially or essentially free from components that normally accompany it in its native state.

By "modulating" is meant increasing or decreasing, either directly or indirectly, the level and/or functional activity of a target molecule. For example, an agent may indirectly modulate the said level/activity by interacting with a molecule other than the target molecule. In this regard, indirect modulation of a gene encoding a target polypeptide includes within its scope modulation of the expression of a first nucleic acid molecule, wherein an expression product of the first nucleic acid molecule modulates the expression of a nucleic acid molecule encoding the target polypeptide.

WO 03/060105 PCT/AU02/01768 -16- By "obtained from is meant that a sample such as, for example, a polynucleotide extract or polypeptide extract is isolated from, or derived from, a particular source of the host. For example, the extract can be obtained from a cell, tissue or a biological fluid isolated directly from the host.

The term "oligonucleotide" as used herein refers to a polymer composed of a multiplicity of nucleotide residues (deoxyribonucleotides or ribonucleotides, or related structural variants or synthetic analogues thereof) linked via phosphodiester bonds (or related structural variants or synthetic analogues thereof). Thus, while the term "oligonucleotide" typically refers to a nucleotide polymer in which the nucleotide residues and linkages between them are naturally occurring, it will be understood that the term also includes within its scope various analogues including, but not restricted to, peptide nucleic acids (PNAs), phosphoramidates, phosphorothioates, methyl phosphonates, 2-O-methyl ribonucleic acids, and the like. The exact size of the molecule can vary depending on the particular application. An oligonucleotide is typically rather short in length, generally from about 10 to 30 nucleotide residues, but the term can refer to molecules of any length, although the term "polynucleotide" or "nucleic acid" is typically used for large oligonucleotides.

By "operably linked" is meant that transcriptional and translational regulatory polynucleotides are positioned relative to a polypeptide-encoding polynucleotide in such a manner that the polynucleotide is transcribed and the polypeptide is translated.

The term "patient" refers to patients of human or other mammal and includes any individual it is desired to examine or treat using the methods of the invention. However, it will be understood that "patient" does not imply that symptoms are present. Suitable mammals that fall within the scope of the invention include, but are not restricted to, primates, livestock animals sheep, cows, horses, donkeys, pigs), laboratory test animals rabbits, mice, rats, guinea pigs, hamsters), companion animals cats, dogs) and captive wild animals foxes, deer, dingoes).

By "pharmaceutically acceptable carrier" is meant a solid or liquid filler, diluent or encapsulating substance that can be safely used in topical or systemic administration to a mammal.

The term "polynucleotide" or "nucleic acid" as used herein designates mRNA, RNA, cRNA, cDNA or DNA. The term typically refers to oligonucleotides greater than nucleotide residues in length.

WO 03/060105 PCT/AU02/01768 17- The terms "polynucleotide variant" and "variant" refer to polynucleotides displaying substantial sequence identity with a reference polynucleotide sequence or polynucleotides that hybridise with a reference sequence under stringent conditions that are defined hereinafter. These terms also encompasses polynucleotides which differ from a reference polynucleotide by the addition, deletion or substitution of at least one nucleotide.

In this regard, it is well understood in the art that certain alterations inclusive of mutations, additions, deletions and substitutions can be made to a reference polynucleotide whereby the altered polynucleotide retains the biological function or activity of the reference polynucleotide. The terms "polynucleotide variant" and "variant" also include naturally occurring allelic variants.

"Polypeptide", "peptide" and "protein" are used interchangeably herein to refer to a polymer of amino acid residues and to variants and synthetic analogues of the same.

Thus, these terms apply to amino acid polymers in which one or more amino acid residues is a synthetic non-naturally occurring amino acid, such as a chemical analogue of a corresponding naturally occurring amino acid, as well as to naturally-occurring amino acid polymers.

The term "polypeptide variant" refers to polypeptides which differ from a reference polypeptide by the addition, deletion or substitution of at least one amino acid. It is well understood in the art for example that some amino acids may be changed to others with broadly similar properties without changing the nature of the activity of the polypeptide (conservative substitutions) as described hereinafter.

By "primer" is meant an oligonucleotide which, when paired with a strand of DNA, is capable of initiating the synthesis of a primer extension product in the presence of a suitable polymerising agent. The primer is preferably single-stranded for maximum efficiency in amplification but can alternatively be double-stranded. A primer must be sufficiently long to prime the synthesis of extension products in the presence of the polymerisation agent. The length of the primer depends on many factors, including application, temperature to be employed, template reaction conditions, other reagents, and source of primers. For example, depending on the complexity of the target sequence, the oligonucleotide primer typically contains 15 to 35 or more nucleotide residues, although it can contain fewer nucleotide residues. Primers can be large polynucleotides, such as from about 200 nucleotide residues to several kilobases or more. Primers can be selected to be "substantially complementary" to the sequence on the template to which it is designed to WO 03/060105 PCT/AU02/01768 -18hybridise and serve as a site for the initiation of synthesis. By "substantially complementary", it is meant that the primer is sufficiently complementary to hybridise with a target polynucleotide. Preferably, the primer contains no mismatches with the template to which it is designed to hybridise but this is not essential. For example, noncomplementary nucleotide residues can be attached to the 5' end of the primer, with the remainder of the primer sequence being complementary to the template. Alternatively, non-complementary nucleotide residues or a stretch of non-complementary nucleotide residues can be interspersed into a primer, provided that the primer sequence has sufficient complementarity with the sequence of the template to hybridise therewith and thereby form a template for synthesis of the extension product of the primer.

"Probe" refers to a molecule that binds to a specific sequence or sub-sequence or other moiety of another molecule. Unless otherwise indicated, the term "probe" typically refers to a polynucleotide probe that binds to another polynucleotide, often called the "target polynucleotide", through complementary base pairing. Probes can bind target polynucleotides lacking complete sequence complementarity with the probe, depending on the stringency of the hybridisation conditions. Probes can be labelled directly or indirectly.

The term "recombinant polynucleotide" as used herein refers to a polynucleotide formed in vitro by the manipulation of a polynucleotide into a form not normally found in nature. For example, the recombinant polynucleotide can be in the form of an expression vector. Generally, such expression vectors include transcriptional and translational regulatory polynucleotide operably linked to the polynucleotide.

By "recombinant polypeptide" is meant a polypeptide made using recombinant techniques, through the expression of a recombinant or synthetic polynucleotide.

By "reporter molecule" as used in the present specification is meant a molecule that, by its chemical nature, provides an analytically identifiable signal that allows the detection of a complex comprising an antigen-binding molecule and its target antigen. The term "reporter molecule" also extends to use of cell agglutination or inhibition of agglutination such as red blood cells on latex beads, and the like.

Terms used to describe sequence relationships between two or more polynucleotides or polypeptides include "reference sequence", "comparison window", "sequence identity", "percentage of sequence identity" and "substantial identity". A "reference sequence" is at least 12 but frequently 15 to 18 and often at least 25 monomer units, inclusive of nucleotides and amino acid residues, in length. Because two WO 03/060105 PCT/AU02/01768 19polynucleotides may each comprise a sequence only a portion of the complete polynucleotide sequence) that is similar between the two polynucleotides, and a sequence that is divergent between the two polynucleotides, sequence comparisons between two (or more) polynucleotides are typically performed by comparing sequences of the two polynucleotides over a "comparison window" to identify and compare local regions of sequence similarity. A "comparison window" refers to a conceptual segment of at least 50 contiguous positions, usually about 50 to about 100, more usually about 100 to about 150 in which a sequence is compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned. The comparison window may comprise additions or deletions gaps) of about 20% or less as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. Optimal alignment of sequences for aligning a comparison window may be conducted by computerised implementations of algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package Release 7.0, Genetics Computer Group, 575 Science Drive Madison, WI, USA) or by inspection and the best alignment resulting in the highest percentage homology over the comparison window) generated by any of the various methods selected. Reference also may be made to the BLAST family of programs as for example disclosed by Altschul et al., 1997, Nucl. Acids Res. 25:3389. A detailed discussion of sequence analysis can be found in Unit 19.3 of Ausubel et al., "Current Protocols in Molecular Biology", John Wiley Sons Inc, 1994-1998, Chapter The term "sequence identity" as used herein refers to the extent that sequences are identical on a nucleotide-by-nucleotide basis or an amino acid-by-amino acid basis over a window of comparison. Thus, a "percentage of sequence identity is calculated by comparing two optimally aligned sequences over the window of comparison, determining the number of positions at which the identical nucleic acid base A, T, C, G, I) or the identical amino acid residue Ala, Pro, Ser, Thr, Gly, Val, Leu, Ile, Phe, Tyr, Trp, Lys, Arg, His, Asp, Glu, Asn, Gin, Cys and Met) occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison the window size), and multiplying the result by 100 to yield the percentage of sequence identity. For the purposes of the present invention, "sequence identity" will be understood to mean the "match percentage" calculated by the DNASIS computer program (Version 2.5 for windows; available from WO 03/060105 PCT/AU02/01768 Hitachi Software engineering Co., Ltd., South San Francisco, California, USA) using standard defaults as used in the reference manual accompanying the software.

"Stringency" as used herein, refers to the temperature and ionic strength conditions, and presence or absence of certain organic solvents, during hybridisation and washing procedures. The higher the stringency, the higher will be the degree of complementarity between immobilised target nucleotide sequences and the labelled probe polynucleotide sequences that remain hybridised to the target after washing.

"Stringent conditions" refers to temperature and ionic conditions under which only nucleotide sequences having a high frequency of complementary bases will hybridise.

The stringency required is nucleotide sequence dependent and depends upon the various components present during hybridisation and subsequent washes, and the time allowed for these processes. Generally, in order to maximise the hybridisation rate, non-stringent hybridisation conditions are selected; about 20 to 250 C lower than the thermal melting point The Tm is the temperature at which 50% of specific target sequence hybridises to a perfectly complementary probe in solution at a defined ionic strength and pH.

Generally, in order to require at least about 85% nucleotide complementarity of hybridised sequences, highly stringent washing conditions are selected to be about 5 to 150 C lower than the Tm. In order to require at least about 70% nucleotide complementarity of hybridised sequences, moderately stringent washing conditions are selected to be about to 300 C lower than the Tm. Highly permissive (low stringency) washing conditions may be as low as 50' C below the Tm, allowing a high level of mis-matching between hybridised sequences. Those skilled in the art will recognise that other physical and chemical parameters in the hybridisation and wash stages can also be altered to affect the outcome of a detectable hybridisation signal from a specific level of homology between target and probe sequences.

The term "transgene" is used herein to describe genetic material that has been or is about to be artificially inserted into the genome or onto an extrachromosomal element of a cell, particularly a bacterial cell and more particularly a Bordetella cell or related cell thereof. The transgene is used to transform a cell, meaning that a permanent or transient genetic change, preferably a permanent genetic change, is induced in a cell following incorporation of exogenous DNA. A permanent genetic change is generally achieved by introduction of the DNA into the genome of the cell. Vectors for stable integration include plasmids, bacteriophages and other bacterial viruses and the like.

WO 03/060105 PCT/AU02/01768 -21 By "vector" is meant a polynucleotide molecule, preferably a DNA molecule derived, for example, from a plasmid, bacteriophage, yeast or virus, into which a polynucleotide can be inserted or cloned. A vector preferably contains one or more unique restriction sites and can be capable of autonomous replication in a defined host cell including a target cell or tissue or a progenitor cell or tissue thereof, or be integrable with the genome of the defined host such that the cloned sequence is reproducible. Accordingly, the vector can be an autonomously replicating vector, a vector that exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, a linear or closed circular plasmid, an extrachromosomal element, a minichromosome, or an artificial chromosome. The vector can contain any means for assuring self-replication. Alternatively, the vector can be one which, when introduced into the host cell, is integrated into the genome and replicated together with the chromosome(s) into which it has been integrated. A vector system can comprise a single vector or plasmid, two or more vectors or plasmids, which together contain the total DNA to be introduced into the genome of the host cell, or a transposon. The choice of the vector will typically depend on the compatibility of the vector with the host cell into which the vector is to be introduced. In the present case, the vector is preferably a bacterial or bacteriophage vector, which is operably functional in a Bordetella strain of interest, or related organism. The vector can also include a selection marker such as an antibiotic resistance gene that can be used for selection of suitable transformants. Examples of such resistance genes are known to those of skill in the art and include the nptll gene that confers resistance to the antibiotics kanamycin and G418 (Geneticin®) and the cat gene which confers resistance to the antibiotic chloramphenicol.

The terms "wild-type" and "normal" are used interchangeably to refer to the phenotype that is characteristic of most of the members of the species occurring naturally and contrast for example with the phenotype of a mutant.

As used herein, underscoring or italicising the name of a gene shall indicate the gene, in contrast to its protein product, which is indicated by the name of the gene in the absence of any underscoring or italicising. For example, "aroQ" shall mean the aroQ gene, whereas "AroQ" shall indicate the protein product of the "aroQ" gene. The terms "AroQ" and "3-dehydroquinase are used interchangeably herein.

WO 03/060105 PCT/AU02/01768 -22- 2. Polynucleotides of the invention 2.1 Bordetella aroO polynucleotides The present invention is predicated in part on the determination of the full-length sequence of the aroQ gene from B. pertussis, which is useful inter alia for the production of genetically modified Bordetella strains of pathogenic origin that are attenuated by disruption or inactivation of their corresponding aroQ gene. The invention, therefore, provides in one aspect an isolated polynucleotide comprising at least a portion of an aroQ gene from a Bordetella species or related organism. Suitably, the polynucleotide comprises the entire sequence of nucleotides set forth in SEQ ID NO: 1. SEQ ID NO: 1 corresponds to a 942 bp genomic sequence for aroQ of B. pertussis. This sequence defines: promoter elements comprising a -35 region from about nucleotide 19 to about nucleotide 24 and (ii) a -10 region from about nucleotide 37 to about nucleotide 42; a ribosome binding site from about nuclcotide 52 to about nucleotide 60; and a coding sequence from about nucleotide 73 to about nucleotide 504 as set forth in SEQ ID NO: 3.

A Bestfit comparison, with standard defaults, of the aroQ gene from B. pertussis and from other microorganisms reveals that the B. pertussis aroQ gene displays 68.9%, 60.6%, 57.0% and 59.9% to the Haemophilus influenzae, Actinobacillus pleuropneumoniae, Helicobacter pylori and Aspergillus nidulans counterpart aroQ genes, respectively.

2.2 Variant aroO polynucleotides The present invention is also directed to variants of the aroQ gene of the present invention. In general, polynucleotide variants according to the invention comprise regions that show at least 50%, more suitably at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90% and still even more preferably at least 95%, 96%, 97%, 98% or 99% sequence identity over a reference polynucleotide sequence of identical size ("comparison window") or when compared to an aligned sequence in which the alignment is performed by a computer homology program known in the art. In one embodiment, the reference polynucleotide is selected from the sequence set forth in SEQ ID NO: 1 or 3.

The aroQ gene sequence, including flanking promoter regions and coding regions, may be modified or mutated in various ways known in the art to generate targeted changes WO 03/060105 PCT/AU02/01768 -23in promoter strength, sequence of the encoded protein, etc. The sequence changes may be substitutions, insertions or deletions. Deletions may include large changes, such as deletions of a domain-encoding region. Other modifications of interest include epitope tagging, e.g. with the FLAG system, HA, etc. For studies of subcellular localisation, fusion proteins with green fluorescent proteins (GFP) may be used. Such mutated genes may be used to study structure-function relationships of AroQ polypeptides, or to alter properties of the proteins that affect their function or regulation.

For example, a polynucleotide according to SEQ ID NO: 1 or 3 can be mutated using random mutagenesis transposon mutagenesis), oligonucleotide-mediated (or site-directed) mutagenesis, PCR mutagenesis and cassette mutagenesis of an earlier prepared variant or non-variant version of an isolated natural promoter according to the invention.

Oligonucleotide-mediated mutagenesis is a preferred method for preparing nucleotide substitution variants of a polynucleotide of the invention. This technique is well known in the art as, for example, described by Adelman et al. (1983, DNA 2:183). Briefly, a polynucleotide according to SEQ ID NO: 1 or 3 is altered by hybridising an oligonucleotide encoding the desired mutation to a template DNA, wherein the template is the single-stranded form of a plasmid or bacteriophage containing the unaltered or parent DNA sequence. After hybridisation, a DNA polymerase is used to synthesise an entire second complementary strand of the template that will thus incorporate the oligonucleotide primer, and will code for the selected alteration in said parent DNA sequence.

Generally, oligonucleotides of at least 25 nucleotides in length are used. An optimal oligonucleotide will have 12 to 15 nucleotides that are completely complementary to the template on either side of the nucleotide(s) coding for the mutation. This ensures that the oligonucleotide will hybridise properly to the single-stranded DNA template molecule.

The DNA template can be generated by those vectors that are either derived from bacteriophage M13 vectors, or those vectors that contain a single-stranded phage origin of replication as described by Viera et al. (1987, Methods Enzymol. 153:3). Thus, the DNA that is to be mutated may be inserted into one of the vectors to generate single-stranded template. Production of single-stranded template is described, for example, in Sections 4.21-4.41 of Sambrook et al. (1989, supra).

Alternatively, the single-stranded template may be generated by denaturing double-stranded plasmid (or other DNA) using standard techniques.

WO 03/060105 PCT/AU02/01768 -24- For alteration of the native DNA sequence, the oligonucleotide is hybridised to the single-stranded template under suitable hybridisation conditions. A DNA polymerising enzyme, usually the Klenow fragment of DNA polymerase I, is then added to synthesise the complementary strand of the template using the oligonucleotide as a primer for synthesis. A heteroduplex molecule is thus formed such that one strand of DNA encodes the mutated form of the polypeptide or fragment under test, and the other strand (the original template) encodes the native unaltered sequence of the polypeptide or fragment under test. This heteroduplex molecule is then transformed into a suitable host cell, usually a prokaryote such as E. coli. After the cells are grown, they are plated onto agarose plates and screened using the oligonucleotide primer having a detectable label to identify the bacterial colonies having the mutated DNA. The resultant mutated DNA fragments are then cloned into suitable expression hosts such as E. coli using conventional technology and clones that retain the desired antigenic activity are detected. Where the clones have been derived using random mutagenesis techniques, positive clones would have to be sequenced in order to detect the mutation.

Alternatively, linker-scanning mutagenesis of DNA may be used to introduce clusters of point mutations throughout a sequence of interest that has been cloned into a plasmid vector. For example, reference may be made to Ausubel et al., supra, (in particular, Chapter 8.4) which describes a first protocol that uses complementary oligonucleotides and requires a unique restriction site adjacent to the region that is to be mutagenised. A nested series of deletion mutations is first generated in the region. A pair of complementary oligonucleotides is synthesised to fill in the gap in .the sequence of interest between the linker at the deletion endpoint and the nearby restriction site. The linker sequence actually provides the desired clusters of point mutations as it is moved or "scanned" across the region by its position at the varied endpoints of the deletion mutation series. An alternate protocol is also described by Ausubel et al., supra, which makes use of site directed mutagenesis procedures to introduce small clusters of point mutations throughout the target region. Briefly, mutations are introduced into a sequence by annealing a synthetic oligonucleotide containing one or more mismatches to the sequence of interest cloned into a single-stranded M13 vector. This template is grown in an E. coli duf ung strain, which allows the incorporation of uracil into the template strand. The oligonucleotide is annealed to the template and extended with T4 DNA polymerase to create a double-stranded heteroduplex. Finally, the heteroduplex is introduced into a wild- WO 03/060105 PCT/AU02/01768 type E. coli strain, which will prevent replication of the template strand due to the presence of apurinic sites (generated where uracil is incorporated), thereby resulting in plaques containing only mutated DNA.

Region-specific mutagenesis and directed mutagenesis using PCR may also be employed to construct polynucleotide variants according to the invention. In this regard, reference may be made, for example, to Ausubel et al., supra, in particular Chapters 8.2A and Alternatively, suitable polynucleotide sequence variants of the invention may be prepared according to the following procedure: creating primers which are optionally degenerate wherein each comprises a portion of a reference polynucleotide including, but not restricted to, a aroQ gene sequence as for example set forth in SEQ ID NO: 1 or 3, or a sequence encoding a reference polypeptide or fragment of the invention, or a complement of said sequence, said polypeptide preferably encoding the sequence set forth in any one of SEQ ID NO: 2; obtaining a nucleic acid extract from an organism, which is preferably a microorganism, and more preferably a bacterium including, but not restricted to, bacteria from the genera Pasteurella, Actinobacillus, Haemophilus, Helicobacter, Aspergillus and Pseudomonus, and still even more preferably a bacterium belonging to the genus Bordetella, or related organism; and using said primers to amplify, via nucleic acid amplification techniques, at least one amplification product from said nucleic acid extract, wherein said amplification product corresponds to a polynucleotide variant.

Suitable nucleic acid amplification techniques are well known to the skilled addressee, and include polymerase chain reaction (PCR) as for example described in Ausubel et al. (supra); strand displacement amplification (SDA) as for example described in U.S. Patent No 5,422,252; rolling circle replication (RCR) as for example described in Liu et al., (1996, J. Am. Chem. Soc. 118:1587-1594 and International application WO 92/01813) and Lizardi et al., (International Application WO 97/19193); nucleic acid sequence-based amplification (NASBA) as for example described by Sooknanan et al., (1994, Biotechniques 17:1077-1080); and Q-j replicase amplification as for example described by Tyagi et al., (1996, Proc. Natl. Acad. Sci. USA 93: 5395-5400).

WO 03/060105 PCT/AU02/01768 -26- Typically, polynucleotide variants that are substantially complementary to a reference polynucleotide are identified by blotting techniques that include a step whereby nucleic acids are immobilised on a matrix (preferably a synthetic membrane such as nitrocellulose), followed by a hybridisation step, and a detection step. Southern blotting is used to identify a complementary DNA sequence; northern blotting is used to identify a complementary RNA sequence. Dot blotting and slot blotting can be used to identify complementary DNA/DNA, DNA/RNA or RNA/RNA polynucleotide sequences. Such techniques are well known by those skilled in the art, and have been described in Ausubel et al. (1994-1998, supra) at pages 2.9.1 through 2.9.20.

According to such methods, Southern blotting involves separating DNA molecules according to size by gel electrophoresis, transferring the size-separated DNA to a synthetic membrane, and hybridising the membrane-bound DNA to a complementary nucleotide sequence labelled radioactively, enzymatically or fluorochromatically. In dot blotting and slot blotting, DNA samples are directly applied to a synthetic membrane prior to hybridisation as above.

An alternative blotting step is used when identifying complementary polynucleotides in a cDNA or genomic DNA library, such as through the process of plaque or colony hybridisation. A typical example of this procedure is described in Sambrook et al. ("Molecular Cloning. A Laboratory Manual", Cold Spring Harbour Press, 1989) Chapters 8-12.

Typically, the following general procedure can be used to determine hybridisation conditions. Polynucleotides are blotted/transferred to a synthetic membrane, as described above. A reference polynucleotide such as a polynucleotide of the invention is labelled as described above, and the ability of this labelled polynucleotide to hybridise with an immobilised polynucleotide is analysed.

A skilled artisan will recognise that a number of factors influence hybridisation.

The specific activity of radioactively labelled polynucleotide sequence should typically be greater than or equal to about 10 8 dpm/mg to provide a detectable signal. A radiolabelled nucleotide sequence of specific activity 108 to 109 dpm/mg can detect approximately 0.5 pg of DNA. It is well known in the art that sufficient DNA must be immobilised on the membrane to permit detection. It is desirable to have excess immobilised DNA, usually gig. Adding an inert polymer such as 10% dextran sulfate (MW 500,000) or WO 03/060105 PCT/AU02/01768 -27polyethylene glycol 6000 during hybridisation can also increase the sensitivity of hybridisation (see Ausubel supra at 2.10.10).

To achieve meaningful results from hybridisation between a polynucleotide immobilised on a membrane and a labelled polynucleotide, a sufficient amount of the labelled polynucleotide must be hybridised to the immobilised polynucleotide following washing. Washing ensures that the labelled polynucleotide is hybridised only to the immobilised polynucleotide with a desired degree of complementarity to the labelled polynucleotide.

It will be understood that polynucleotide variants according to the invention will hybridise to a reference polynucleotide under at least low stringency conditions. Reference herein to low stringency conditions includes and encompasses from at least about 1% v/v to at least about 15% v/v formamide and from at least about 1 M to at least about 2 M salt for hybridisation at 420 C, and at least about 1 M to at least about 2 M salt for washing at 420 C. Low stringency conditions also may include 1% Bovine Serum Albumin (BSA), 1 mM EDTA, 0.5 M NaHPO 4 (pH 7% SDS for hybridisation at 650 C, and 2xSSC, 0.1% SDS; or (ii) 0.5% BSA, 1 mM EDTA, 40 mM NaHPO 4 (pH 5% SDS for washing at room temperature.

Suitably, the polynucleotide variants hybridise to a reference polynucleotide under at least medium stringency conditions. Medium stringency conditions include and encompass from at least about 16% v/v to at least about 30% v/v formamide and from at least about 0.5 M to at least about 0.9 M salt for hybridisation at 420 C, and at least about 0.1 M to at least about 0.2 M salt for washing at 550 C. Medium stringency conditions also may include 1% Bovine Serum Albumin (BSA), 1 mM EDTA, 0.5 M NaHPO 4 (pH 7.2), 7% SDS for hybridisation at 650 C, and 2 x SSC, 0.1% SDS; or (ii) 0.5% BSA, 1 mM EDTA, 40 mM NaHPO 4 (pH 5% SDS for washing at 60-650 C.

Preferably, the polynucleotide variants hybridise to a reference polynucleotide under high stringency conditions. High stringency conditions include and encompass from at least about 31% v/v to at least about 50% v/v formamide and from about 0.01 M to about 0.15 M salt for hybridisation at 420 C, and about 0.01 M to about 0.02 M salt for washing at 550 C. High stringency conditions also may include 1% BSA, 1 mM EDTA, M NaHPO 4 (pH 7% SDS for hybridisation at 650 C, and 0.2 x SSC, 0.1% SDS; or (ii) 0.5% BSA, 1mM EDTA, 40 mM NaHPO 4 (pH 1% SDS for washing at a temperature in excess of 650 C.

WO 03/060105 PCT/AU02/01768 -28- Other stringent conditions are well known in the art. A skilled addressee will recognise that various factors can be manipulated to optimise the specificity of the hybridisation. Optimisation of the stringency of the final washes can serve to ensure a high degree of hybridisation. For detailed examples, see Ausubel et al., supra at pages 2.10.1 to 2.10.16 and Sambrooket al. (1989, supra) at sections 1.101 to 1.104.

While stringent washes are typically carried out at temperatures from about 420 C to 680 C, one skilled in the art will appreciate that other temperatures may be suitable for stringent conditions. Maximum hybridisation rate typically occurs at about 200 C to 250 C below the Tm for formation of a DNA-DNA hybrid. It is well known in the art that the Tm is the melting temperature, or temperature at which two complementary polynucleotide sequences dissociate. Methods for estimating Tm are well known in the art (see Ausubel et al., supra at page 2.10.8).

In general, the Tm of a perfectly matched duplex of DNA may be predicted as an approximation by the formula: T, 81.5 16.6 (logio M) 0.41 0.63 formamide) (600/length) wherein: M is the concentration of Na preferably in the range of 0.01 molar to 0.4 molar; %G+C is the sum of guanosine and cytosine bases as a percentage of the total number of bases, within the range between 30% and 75% G+C; formamide is the percent formamide concentration by volume; length is the number of base pairs in the DNA duplex.

The Tm of a duplex DNA decreases by approximately 10 C with every increase of 1% in the number of randomly mismatched base pairs. Washing is generally carried out at Tm 150 C for high stringency, or Tm 300 C for moderate stringency.

In a preferred hybridisation procedure, a membrane a nitrocellulose membrane or a nylon membrane) containing immobilised DNA is hybridised overnight at 42° C in a hybridisation buffer (50% deionised formamide, 5xSSC, 5x Denhardt's solution ficoll, 0.1% polyvinylpyrollidone and 0.1% bovine serum albumin), 0.1% SDS and 200 mg/mL denatured salmon sperm DNA) containing labelled probe. The membrane is then subjected to two sequential medium stringency washes 2xSSC, 0.1% SDS for min at 450 C, followed by 2xSSC, 0.1% SDS for 15 min at 500 followed by two sequential higher stringency washes 0.2xSSC, 0.1% SDS for 12 min at 550 C followed by 0.2xSSC and 0.1%SDS solution for 12 min at 65-680 C).

WO 03/060105 PCT/AU02/01768 -29- Methods for detecting a labelled polynucleotide hybridised to an immobilised polynucleotide are well known to practitioners in the art. Such methods include autoradiography, phosphorimaging, and chemiluminescent, fluorescent and colorimetric detection.

For the most part, DNA fragments will be of at least 15 nucleotides, usually at least 18 nucleotides, more usually at least about 50 nucleotides. Such small DNA fragments are useful as primers for PCR, hybridisation screening, etc. Larger DNA fragments, i.e. greater than 100 nucleotides are useful for production of the encoded polypeptide. For use in amplification reactions, such as PCR, a pair of primers will be used.

3. Genetically modified Bordetella strains The subject genetically modified Bordetella strains of pathogenic origin typically, but not exclusively, comprise a foreign or exogenous polynucleotide sequence or transgene present as an extrachromosomal element or integrated in the bacterial chromosome. The Bordetella strains are preferably, but not exclusively, selected from Bordetella avium, Bordetella bronchiseptica, Bordetella holmesii, Bordetella parapertussis and Bordetella pertussis. The genetically modified Bordetella strains that may be constructed in accordance with the present invention generally fall into two groups, colloquially termed "knockouts" and "knockins". In the present invention, knockouts have a partial or complete loss of function in the endogenous aroQ gene. Knockins have an introduced transgene foreign gene) with altered genetic sequence and function from the endogenous gene. Increased (including ectopic) or decreased expression may be achieved by introduction of an additional copy of the target gene, or by operatively inserting a regulatory sequence that provides for enhanced expression of an endogenous copy of the target gene. These changes may be constitutive or conditional, i.e. dependent on the presence of an activator or repressor. The foreign gene is usually either from a different species than the host, or is otherwise altered in its coding or non-coding sequence. The introduced gene may be a wild-type gene, naturally occurring polymorphism, or a genetically manipulated sequence, for example having deletions, substitutions or insertions in the coding or non-coding regions. The introduced sequence may encode an AroQ polypeptide, or may utilise the aroQ promoter operably linked to a reporter gene. Where the introduced gene is a coding sequence, it is usually operably linked to a promoter, which WO 03/060105 PCT/AU02/01768 may be constitutive or inducible, and other regulatory sequences required for expression in the host bacterium. A knockin and a knockout may be combined, such that the naturally occurring gene is disabled, and an altered form introduced.

Preferably, a genetically modified Bordetella strain of the present invention has a partial or complete loss of function in the endogenous aroQ gene and thus falls into the "knockout" group of genetically modified organisms. A knockout may be achieved by a variety of mechanisms, including introduction of a disruption of the coding sequence, e.g.

insertion of one or more stop codons, insertion of a DNA fragment, etc., deletion of coding sequence, substitution of stop codons for coding sequence, etc. In some cases the foreign transgene sequences are ultimately deleted from the genome, leaving a net change to the native sequence. Different approaches may be used to achieve the "knockout". A chromosomal deletion of all or part of the native aroQ may be induced, including deletions of the non-coding regions, particularly the promoter region, 3' regulatory sequences, transcriptional regulators, or deletion of a gene that activates expression of aroQ. A functional knockout may also be achieved by the introduction of an anti-sense construct (anti-sense suppression) or a sense construct (co-suppression) that block expression of the native aroQ gene. "Knockouts" also include conditional knock-outs, for example where alteration of the target gene occurs upon exposure of the Bordetella strain to a substance that promotes target gene alteration, introduction of an enzyme that promotes recombination at the target gene site Cre in the Cre-lox system), or other method for directing the target gene alteration.

In a preferred embodiment, the partial or complete loss of function in the aroQ gene is effected by disruption of that gene. In accordance with the present invention, the disruption suitably results in an inability of the genetically modified Bordetella strain to produce a corresponding functional expression product or detectable levels of that expression product. Accordingly, a disruption in the endogenous aroQ gene may result in a reduced level and/or functional activity of the 3-dehydroquinase (AroQ) encoded by the aroQ gene or in an inability of the strain to produce a functional 3-dehydroquinase or detectable levels of 3-dehydroquinase relative to a corresponding cell without the disruption.

A disruption typically comprises an insertion of a nucleic acid sequence into one region of the native genomic sequence (usually the aroQ open reading frame) and/or the promoter region of a gene so as to decrease or prevent expression of that gene in the cell as WO 03/060105 PCT/AU02/01768 -31 compared to the wild-type or naturally occurring sequence of the gene. By way of example, a nucleic acid construct can be prepared containing a selectable marker gene which is inserted into a targeting nucleic acid sequence that is complementary to a genomic sequence (promoter and/or coding region) to be disrupted. Useful genomic sequences to be disrupted include, but are not restricted to, aroQ open reading frames encoding polypeptides or domains and adjacent 5' and 3' non-coding nucleotide sequences involved in regulation of gene expression. Accordingly, a targeting sequence may comprise some or part of the nucleic acid present between the initiation codon and the stop codon of the aroQ open reading frame, as defined in the listed sequences. It may further include the 3' and untranslated regions found in the mRNA. It may further include specific transcriptional and translational regulatory sequences, such as promoters, operators, etc., including about 1 kb, but possibly more, of flanking genomic DNA at either the 5' or 3' end of the transcribed region. When the nucleic acid construct is then introduced into a cell, the construct will integrate into the genomic DNA. Thus, many progeny of the cell will no longer express the gene at least in some cells, or will express it at a decreased level, as the genomic sequence is now disrupted by the selection marker.

In another embodiment, an individual disruption reduces, abrogates or otherwise impairs the expression of a aroQ gene and in this regard, the disruption may reside in the deletion of at least a portion of the transcriptional and/or translational regulatory sequences associated with said aroQ gene.

In a preferred embodiment, the disruption comprises a defined deletion in the aroQ gene. The deletion in the aroQ gene can be introduced by allelic exchange due to a double cross-over recombinational event, or any other method wherein a DNA replacement event in which two separate DNA recombination events result in the exchange of a piece of the intact gene for a homologous piece containing a deletion. The deletion is preferably large enough such that the gene is inactivated in the first attempt and to reduce the likelihood of a recombinational repair. Other genes, or at least portions thereof, which can be deleted include the pur genes purA, purE or purH), other aro genes aroA, aroB, aroC or aroE, or pertussis toxin gene, or any other gene which contributes to survival in the host and/or to bacterial virulence, or any combination of such genes. Any strain of Bordetella can be used to introduce such an attenuating mutation. The resulting attenuated strain can be tested for the deletion of the targeted gene by methods known in the art such as Southern blot hybridisation, and the level of attenuation tested in a mouse WO 03/060105 PCT/AU02/01768 -32model as described in the Examples below. Any deletion in the aroQ gene would result in the attenuation of the bacteria unless the deletion was small and allowed for functional aroQ expression. Any deletion which inactivates the aroQ gene expression or blocks function of its gene product will result in sufficient attenuation of a pathogenic strain of Bordetella such that it has a lower capacity to propagate in a mammalian host but remain viable in the host for a period of time sufficient to induce an immune response against a pathogenic Bordetella strain and preferably the same strain that was the subject of attenuation In another embodiment of the present invention, the genetically modified Bordetella strain having the deletion in the aroQ gene as described above additionally contains another deletion in a different gene. The advantage of having two deletions is to further reduce the possibility of reversion, and to additionally attenuate the bacteria.

However, for use as an immunopotentiating composition or live vaccine, a certain amount of replication is necessary in the host. Therefore, in a preferred embodiment, the genetically modified strain is tested for its ability to survive in the host. These tests can be carried out in vivo, for example in the mouse model described herein, or, as a second step, in non-human primates.

In yet another embodiment, the genetically modified Bordetella strain of the present invention may be used as a vector or delivery vehicle of antigens from organisms other than said Bordetella strain. Illustrative embodiments of such heterologous antigens to be expressed by the genetically modified Bordetella strain of the present invention include, antigens bacteria, viruses, fungi, protozoa, metazoan parasites or the like. The heterologous structural genes may encode envelope proteins, capsid proteins, surface proteins such as outer membrane proteins and capsids, toxins such as exotoxins or enterotoxins, or the genes of interest may specify proteins, enzymes, or oligosaccharide antigen or for modification of a saccharide-containing antigen, such as LPS, of the host bacterial strain, or for synthesis of a polypeptide antigen, or variants or derivatives thereof. Specific examples of genes of interest include HIV glycoproteins, malarial circumsporozoite protein, HBV core protein, and arboviral coat protein, to name a few. Alternatively, or additionally, the heterologous antigen may be selected from disease associated antigens such as cancer-related antigens tumour-associated antigens such as the melanomaassociated MAGE antigens). In a preferred embodiment, the heterologous antigen is from other respiratory bacterial pathogens such as, but not limited to, Helicobacter pylori, WO 03/060105 PCT/AU02/01768 -33- Haemophilus influenzae and Neisseria meningitidis or from viral respiratory pathogens including those associated with measles, mumps or rubella.

One or more structural genes coding for desired heterologous antigen(s) may be introduced into the genetically modified Bordetella strain as an expression cassette. The expression cassette comprises the heterologous gene or genes of interest under the regulatory control of the transcriptional and translational initiation and termination elements which naturally border the gene of interest. Where bacterial or bacteriophage heterologous genes are involved, the natural or wild-type regulatory regions will usually, but not always, suffice. It may be necessary to join regulatory regions recognised by the genetically modified Bordetella strain to structural genes for antigens isolated from eukaryotes and occasionally prokaryotes.

The expression cassette may be a construct or may form part of a naturally occurring plasmid. If the expression cassette is a construct, it may be joined to a replication system for episomal maintenance or it may be introduced into the genetically modified Bordetella strain under conditions for recombination and integration into said strain's chromosomal DNA.

4. Targeting constructs The invention provides a targeting vector for producing a genetically modified Bordetella strain of the present invention, comprising at least a portion of a polynucleotide of the invention or variant or derivative of thereof. Specific constructs or vectors of interest include, but are not limited to, anti-sense aroQ constructs comprising a sequence complementary to a polynucleotide, fragment, variant or derivative as herein described, which will block native aroQ expression and expression of dominant negative AroQ mutations.

In a preferred embodiment, the vector is used to disrupt the aroQ gene, which results in partial or complete loss of function of that gene. Any polynucleotide sequence capable of disrupting an endogenous gene of interest by introducing a deletion, insertion or replacement of at least one nucleotide, and preferably from 10 to 20 nucleotides therein, resulting in a nonsense mutation, a missense mutation or a frame-shift mutation) may be employed in this regard. In a preferred embodiment, the vector, or an ancillary vector, comprises a positive selectable marker gene. The disruption may reduce or prevent the expression of aroQ or may render the resulting AroQ polypeptide completely non- WO 03/060105 PCT/AU02/01768 -34functional. Reduced levels of AroQ refer to a level of AroQ which is lower than that found in a wild-type Bordetella strain. The level of AroQ produced in a Bordetella strain of interest may be determined by a variety of methods including Western blot analysis of protein extracted from that strain. A lack of ability to produce functional AroQ includes within its scope the production of undetectable levels of functional AroQ by Western blot analysis). In contrast, a functional AroQ is a molecule which retains the biological activity of the wild-type AroQ and which preferably is of the same molecular weight as the wild-type molecule.

Targeting vectors for homologous recombination allelic exchange) will comprise at least a portion of the aroQ gene with the desired genetic modification, and will include regions of homology to the target locus. Those regions may be non-isogenic, but are preferably isogenic, to the target locus. Conveniently, markers for positive and negative selection are included. Methods for generating cells having targeted gene modifications through homologous recombination are known in the art.

In certain embodiments, the targeting construct may contain more than one selectable marker gene. The selectable marker is preferably a polynucleotide which encodes an enzymatic activity that confers resistance to an antibiotic or drug upon the cell in which the selectable marker is expressed. Selectable markers may be "positive"; positive selectable markers typically are dominant selectable markers, genes which encode an enzymatic activity which can be detected in any bacterial cell. Examples of dominant selectable markers include the bacterial aminoglycoside 3' phosphotransferase gene (also referred to as the kan gene) which confers resistance to the kanamycin (KmR), the bacterial chloramphenicol acetyl transferase gene (also referred to as the cat gene) which confers resistance to the chloramphenicol (CmR). Selectable markers may be 'negative'; negative selectable markers (or otherwise called 'counter-selectable markers') encode an enzymatic activity whose expression is cytotoxic to the cell when grown in an appropriate selective medium, such as but not limited to a gene encoding E. coli S12 ribosomal protein, which renders a streptomycin resistant (Sm

R

host streptomycin sensitive (Sm s or a sacB gene as for example described by Pelicic et al. (1996, Mol. Microbiol. 20: 919-925).

When more than one selectable marker gene is employed, the targeting vector preferably contains a positive selectable marker the kan gene) and a counterselectable marker S12 gene). The presence of the positive selectable marker permits the selection of recombinant cells containing an integrated copy of the targeting vector WO 03/060105 PCT/AU02/01768 whether this integration occurred at the target site or at a random site. The presence of the counter-selectable marker permits the identification of cells containing the targeting vector at the targeted site which has integrated by virtue of homologous recombination into the target site); cells which survive when grown in medium which selects against the expression of the counter-selectable marker do not contain a copy of the counter-selectable marker.

Preferred targeting vectors of the present invention are of the "replacement-type", wherein integration of a replacement-type vector results in the insertion of a selectable marker into the target gene. Replacement-type targeting vectors may be employed to disrupt a gene resulting in the generation of a null gene a gene incapable of expressing a functional protein; null genes may be generated by deleting a portion of the coding region, deleting the entire gene, introducing an insertion and/or a frameshift mutation, etc.) or may be used to introduce a modification one or more point mutations) into a gene.

The targeting construct or vector is then introduced into a host strain by any convenient means such as, but not limited to, conjugation, transformation, transfection, transduction, translocation, etc.

In a particularly preferred embodiment, as described in more detail below, a replacement-type targeting vector was used to introduce a deletion in the aroQ gene of Bordetella pertussis by allelic exchange with a copy of the aroQ gene present on the vector. The aroQ gene to be exchanged with the wild type version contained a deletion into which a kanamycin resistance gene was cloned. A copy of the S12 gene was also present on the vector. Once the vector was introduced into Bordetella pertussis, by conjugation in this example, a double cross-over recombinational event occurred such that the vector aroQ gene containing the deletion, was exchanged for the chromosomal wild type aroQ.

The resulting recombinant Bordetella pertussis strain was KmR, Sm s and contained a defined mutation which conferred attenuation as described herein.

Polypeptides of the invention 5.1 Bordetella AroQ polypeptides The invention encompasses the use of AroQ polypeptides from Bordetella in immunopotentiating compositions. For example, such AroQ polypeptides may be used in WO 03/060105 PCT/AU02/01768 -36concert with the genetically modified Bordetella strain of the present invention to elicit an immune response against Bordetella strains of pathogenic origin. An exemplary Bordetella pertussis sequence for AroQ is set forth in SEQ ID NO: 2.

5.2 Biologically active fragments Biologically active fragments may be produced according to any suitable procedure known in the art. For example, a suitable method may include first producing a fragment of said polypeptide and then testing the fragment for the appropriate biological activity. In one embodiment, the fragment may be tested for 3-dehydroquinase activity. In another embodiment, biological activity of the fragment is tested by introducing a polynucleotide from which a fragment of the polypeptide can be translated into a cell, and detecting 3-dehydroquinase activity, which is indicative of the fragment being a biologically active fragment.

The invention also contemplates biological fragments of the above polypeptides of at least 6 and preferably at least 8 amino acids in length, which can elicit an immune response in an animal for the production of antigen-binding molecules that are interactive with a 3-dehydroquinase of the invention. For example exemplary polypeptide fragments of 8 residues in length, which could elicit an immune response, include but are not limited to residues 1-8, 9-16, 17-24, 25-32, 33-40, 41-48, 49-56, 57-64, 65-72, 73-80, 81-88, 89- 96, 97-104, 105-112, 113-120, 121-128, 129-136 and 137-144 of SEQ ID NO: 2.

5.3 Variant AroO polypeptides The invention contemplates the use of variants of AroQ polypeptides from Bordetella in immunopotentiating compositions. Suitable methods of producing polypeptide variants include replacing at least one amino acid of a parent polypeptide comprising the sequence set forth in SEQ ID NO: 2, or a biologically active fragment thereof, with a different amino acid to produce a modified polypeptide, and testing said modified polypeptide for an activity of the parent AroQ polypeptide, including 3dehydroquinase activity, which indicates that the modified polypeptide is a polypeptide variant.

In another embodiment, a polypeptide variant is produced by replacing at least one amino acid of a parent polypeptide comprising the sequence set forth in SEQ ID NO: 2, or a biologically active fragment thereof, with a different amino acid to produce a WO 03/060105 PCT/AU02/01768 -37modified polypeptide, introducing said polypeptide or a polynucleotide from which the modified polypeptide can be translated into a cell having a deletion of, or disruption in, the corresponding aroQ gene, and detecting an activity of the parent AroQ polypeptide, including 3-dehydroquinase activity, which indicates that the modified polypeptide is a polypeptide variant. For example, one may test the recombinant cell for growth in the absence of aromatic amino acids.

In general, variants will be at least 50%, preferably at least 55%, more preferably at least 60%, even more preferably at least 65%, even more preferably at least 70%, even more preferably at least 75%, even more preferably at least 80%, even more preferably at least 85%, even more preferably at least 90% and still even more preferably at least homologous to a polypeptide as for example shown in SEQ ID NO: 2, or in fragments thereof. Suitably, variants will have at least 50%, preferably at least 55%, more preferably at least 60%, even more preferably at least 65%, even more preferably at least 70%, even more preferably at least 75%, even more preferably at least 80%, even more preferably at least 85%, even more preferably at least 90% and still even more preferably at least sequence identity to the sequence set forth in SEQ ID NO: 2.

Variant peptides or polypeptides, resulting from rational or established methods of mutagenesis or from combinatorial chemistries, for example, may comprise conservative am.no acid substitutions. Exemplary conservative substitutions in a polypeptide or polypeptide fragment according to the invention may be made according to the following table: TABLE B Original Residue Exemplary Substitutions Ala Ser Arg Lys Asn Gin, His Asp Glu Cys Ser Gin Asn Glu Asp Gly Pro WO 03/060105 PCT/AU02/01768 -38- Original Residue Exemplary Substitutions His Asn, Gin lie Leu, Val Leu Ile, Val Lys Arg, Gin, Glu Met Leu, Ile, Phe Met, Leu, Tyr Ser Thr Thr Ser Trp Tyr Tyr Trp, Phe Val Ile, Leu Substantial changes in function are made by selecting substitutions that are less conservative than those shown in TABLE B. Other replacements would be nonconservative substitutions and relatively fewer of these may be tolerated. Generally, the substitutions which are likely to produce the greatest changes in a polypeptide's properties are those in which a hydrophilic residue Ser or Asn) is substituted for, or by, a hydrophobic residue Ala, Leu, Ile, Phe or Val); a cysteine or proline is substituted for, or by, any other residue; a residue having an electropositive side chain Arg, His or Lys) is substituted for, or by, an electronegative residue Glu or Asp) or a residue having a smaller side chain Ala, Ser) or no side chain Gly) is substituted for, or by, one having a bulky side chain Phe or Trp).

5.4 Polypeptide derivatives With reference to suitable derivatives of the invention, such derivatives include amino acid deletions and/or additions to a polypeptide, fragment or variant of the invention, wherein said derivatives comprise an activity of an AroQ polypeptide, including 3-dehyroquinase activity. "Additions" of amino acids may include fusion of the polypeptides, fragments and polypeptide variants of the invention with other polypeptides or proteins. For example, it will be appreciated that said polypeptides, fragments or WO 03/060105 PCT/AU02/01768 -39variants may be incorporated into larger polypeptides, and that such larger polypeptides may also be expected to modulate an activity as mentioned above.

The polypeptides, fragments or variants of the invention may be fused to a further protein, for example, which is not derived from the original host. The further protein may assist in the purification of the fusion protein. For instance, a polyhistidine tag or a maltose binding protein may be used in this respect as described in more detail below. Other possible fusion proteins are those which produce an immunomodulatory response.

Particular examples of such proteins include Protein A or glutathione S-transferase (GST).

Other derivatives contemplated by the invention include, but are not limited to, modification to side chains, incorporation of unnatural amino acids and/or their derivatives during peptide, polypeptide or protein synthesis and the use of crosslinkers and other methods which impose conformational constraints on the polypeptides, fragments and variants of the invention. Examples of side chain modifications contemplated by the present invention include modifications of amino groups such as by acylation with acetic anhydride; acylation of amino groups with succinic anhydride and tetrahydrophthalic anhydride; amidination with methylacetimidate; carbamoylation of amino groups with cyanate; pyridoxylation of lysine with pyridoxal-5-phosphate followed by reduction with NaBH 4 reductive alkylation by reaction with an aldehyde followed by reduction with NaBH 4 and trinitrobenzylation of amino groups with 2, 4, 6-trinitrobenzene sulphonic acid (TNBS). The carboxyl group may be modified by carbodiimide activation via 0acylisourea formation followed by subsequent derivatisation, by way of example, to a corresponding amide. The guanidine group of arginine residues may be modified by formation of heterocyclic condensation products with reagents such as 2,3-butanedione, phenylglyoxal and glyoxal. Sulphydryl groups may be modified by methods such as performic acid oxidation to cysteic acid; formation of mercurial derivatives using 4chloromercuriphenylsulphonic acid, 4-chloromercuribenzoate; 2-chloromercuri-4nitrophenol, phenylmercury chloride, and other mercurials; formation of a mixed disulphides with other thiol compounds; reaction with maleimide, maleic anhydride or other substituted maleimide; carboxymethylation with iodoacetic acid or iodoacetamide; and carbamoylation with cyanate at alkaline pH. Tryptophan residues may be modified, for example, by alkylation of the indole ring with 2-hydroxy-5-nitrobenzyl bromide or sulphonyl halides or by oxidation with N-bromosuccinimide. Tyrosine residues may be modified by nitration with tetranitromethane to form a 3-nitrotyrosine derivative. The WO 03/060105 WO 03/60105PCT/AU02/01768 imidazole ring of a histidine residue may be modified by N-carbethoxylation with diethylpyrocarbonate or by alkylation with iodoacetic acid derivatives.

Examples of incorporating unnatural amino acids and derivatives during peptide synthesis include but are not limited to, use of 4-amino butyric acid, 6-aminohexanoic acid, 4-amnino-3-hydroxy-5-phenylpentanoic acid, 4-amnino-3-hydroxy-6-methylheptanoic acid, t-butylglycine, norleucine, norvaline, phenyiglycine, ornithine, sarcosine, 2-thienyl alanine and/or D-isomers of amino acids. A list of unnatural amino acids contemplated by the present invention is shown in TABLE C.

TABLE C Non-conventional amino acid a-aminobutyric acid a-amino-cx-methylbutyrate aminocyclopropane-carboxylate aminoisobutyric acid amninonorbornyl-carboxylate cyclohexylalanine cyclopentylalanine L-N-methylisoleucine D-alanine D-arginine D-aspartic acid D-cysteine D-glutamate D-glutamic acid D-histidine D-isoleucine D-leucine D-lysine D-methionine D-omnithine Non-conventional amino acid L-N-methylalanine L-N-methylarginine L-N-methylasparaginc L-N-methylaspartic acid L-N-methylcysteine L-N-methylglutamine L-N-methylglutamic acid L-N-methythistidine L-N-methylleucine L-N-methyllysine L-N-methylmethionine L-N-methylnorleucine L-N-methylnorvaline L-N-methylormthine L-N-methylphenylalanine L-N-methylproline L-N-medlylserine L-N-methylthreonine L-N-methyltryptophan L-N-methyltyrosine WO 03/060105 WO 03/60105PCTIAU02/01768 41 Non-conventional amino acid D-phenylalanine D-proline D-serine D-threonine D-tryptophan D-tyrosine D-valine D-ce-methylalanine D-a-methylarginine D-a-methylasparagine D-a-methylaspartate D-c-methylcysteine D-ca-methylglutamine D-oa-methylhisidine D-a-methylisoleucine D-a-methylleucine D-a-methyllysine D-a-methylmethionine D-tx-methylomithfine D-ca-methylphenylalanine D-a-methylproline D-a-methylserine D-ca-methylthreonine D-a-methyltryptophan D-a-methyltyrosine L-ca-methylleucine L-x-methylmethionine L-rr-rethylnorvatine L-cu-methylphenylalanine Non-conventional amino acid L-N-methylvaline L-N-methylethylglycine L-N-methyl--butylglycine L-norleucine L-norvaline a-methyl-aminoisobutyrate a-methyl-'yaminobutyrate a-methylcyclohexylalanine ci-methylcylcopentylalanine a-methyl-u-napthylalanine a-methylpenicillamnine N-(4-aminobutyl)glycine N-(2-aminoethyl)glycine N-(3-aminopropyl)glycine N-amino-a-methylbutyrate a-napthylalanine N-benzylglycine N-(2-carbamylediyl)glycine N-(carbamylmethyl)glycine N-(2-carboxyethyl)glycine N-(carboxyrnethyl)glycine N-cyclobutylglycine N-cycloheptylglycine N-cyclohexylglycine N-cyclodecylglycine L-a-methyllysine L-a-methylnorleucine L-a-methylomithine L-a-methylproline WO 03/060105 PCT/AU02/01768 -42- Non-conventional amino acid Non-conventional amino acid L-a-methylserine L-a-methylthreonine L-a-methyltryptophan L-a-methyltyrosine L-a-methylvaline L-N-methylhomophenylalanine N-(N-(2,2-diphenylethyl N-(N-(3,3-diphenylpropyl carbamylmethyl)glycine carbamylmethyl)glycine 1 -carboxy-l-(2,2-diphenyl-ethyl amino)cyclopropane Also contemplated is the use of crosslinkers, for example, to stabilise 3D conformations of the polypeptides, fragments or variants of the invention, using homobifunctional cross linkers such as bifunctional imido esters having (CH 2 )n spacer groups with n 1 to n 6, glutaraldehyde, N-hydroxysuccinimide esters and hetero-bifunctional reagents which usually contain an amino-reactive moiety such as N-hydroxysuccinimide and another group specific-reactive moiety such as maleimido or dithio moiety or carbodiimide. In addition, peptides can be conformationally constrained, for example, by introduction of double bonds between Ca and Cp atoms of amino acids, by incorporation of Ca and Na-methylamino acids, and by formation of cyclic peptides or analogues by introducing covalent bonds such as forming an amide bond between the N and C termini between two side chains or between a side chain and the N or C terminus of the peptides or analogues. For example, reference may be made to: Marlowe (1993, Biorganic Medicinal Chemistry Letters 3: 437-44) who describes peptide cyclisation on TFA resin using trimethylsilyl (TMSE) ester as an orthogonal protecting group; Pallin and Tam (1995, J. Chem. Soc. Chem. Comm. 2021-2022) who describe the cyclisation of unprotected peptides in aqueous solution by oxime formation; Algin et al (1994, Tetrahedron Letters 35: 9633-9636) who disclose solid-phase synthesis of head-to-tail cyclic peptides via lysine side-chain anchoring; Kates et al (1993, Tetrahedron Letters 34: 1549-1552) who describe the production of head-to-tail cyclic peptides by threedimensional solid phase strategy; Tumelty et al (1994, J. Chem. Soc. Chem. Comm. 1067- 1068) who describe the synthesis of cyclic peptides from an immobilised activated intermediate, wherein activation of the immobilised peptide is carried out with Nprotecting group intact and subsequent removal leading to cyclisation; McMurray et al (1994, Peptide Research 7: 195-206) who disclose head-to-tail cyclisation of peptides WO 03/060105 PCT/AU02/01768 -43attached to insoluble supports by means of the side chains of aspartic and glutamic acid; Hruby et al (1994, Reactive Polymers 22: 231-241) who teach an alternate method for cyclising peptides via solid supports; and Schmidt and Langer (1997, J. Peptide Res. 49: 67-73) who disclose a method for synthesising cyclotetrapeptides and cyclopentapeptides.

The foregoing methods may be used to produce conformationally constrained polypeptides that modulate reproductive function.

The invention also contemplates polypeptides, fragments or variants of the invention that have been modified using ordinary molecular biological techniques so as to improve their resistance to proteolytic degradation or to optimise solubility properties or to render them more suitable as an immunogenic agent.

Methods of preparing AroO polypeptides AroQ polypeptides, fragments, variants or derivatives may be prepared by any suitable procedure known to those of skill in the art. For example, such polypeptides may be prepared by a procedure including the steps of preparing a recombinant polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising the sequence set forth in any one of SEQ ID NO: 2, or a biologically active fragment thereof, or variant or derivative of these, which nucleotide sequence is operably linked to regulatory elements; introducing the recombinant polynucleotide into a suitable host cell; culturing the host cell to express recombinant polypeptide from the recombinant polynucleotide; and isolating the recombinant polypeptide. Preferred nucleotide sequences include, but are not limited to the sequences set forth in SEQ ID NO: 1 or 3.

The recombinant polynucleotide is preferably in the form of an expression vector that may be a self-replicating extra-chromosomal vector such as a plasmid, or a vector that integrates into a host genome. The regulatory elements will generally be appropriate for the host cell used for expression. Numerous types of appropriate expression vectors and suitable regulatory sequences are known in the art for a variety of host cells. Typically, the regulatory elements include, but are not limited to, promoter sequences, leader or signal sequences, ribosomal binding sites, transcriptional start and stop sequences, translational start and termination sequences, and enhancer or activator sequences. Constitutive or inducible promoters as known in the art are contemplated by the invention. The promoters may be either naturally occurring promoters, or hybrid promoters that combine elements of more than one promoter.

WO 03/060105 PCT/AU02/01768 -44- In a preferred embodiment, the expression vector contains a selectable marker gene to allow the selection of transformed host cells. Selection genes are well known in the art and will vary with the host cell used.

The expression vector may also include a fusion partner (typically provided by the expression vector) so that the recombinant polypeptide of the invention is expressed as a fusion polypeptide with said fusion partner. The main advantage of fusion partners is that they assist identification and/or purification of said fusion polypeptide. In order to express said fusion polypeptide, it is necessary to ligate a polynucleotide according to the invention into the expression vector so that the translational reading frames of the fusion partner and the polynucleotide coincide. Well known examples of fusion partners include, but are not limited to, glutathione-S-transferase (GST), Fc potion of human IgG, maltose binding protein (MBP) and hcxahistidinc (HIS 6 which are particularly useful for isolation of the fusion polypeptide by affinity chromatography. For the purposes of fusion polypeptide purification by affinity chromatography, relevant matrices for affinity chromatography are glutathione-, amylose-, and nickel- or cobalt-conjugated resins respectively. Many such matrices are available in "kit" form, such as the QIAexpressTM system (Qiagen) useful with

(HIS

6 fusion partners and the Pharmacia GST purification system. In a preferred embodiment, the recombinant polynucleotide is expressed in the commercial vector pFLAG as described more fully hereinafter. Another fusion partner well known in the art is green fluorescent protein (GFP). This fusion partner serves as a fluorescent "tag" which allows the fusion polypeptide of the invention to be identified by fluorescence microscopy or by flow cytometry. The GFP tag is useful when assessing subcellular localisation of the fusion polypeptide of the invention, or for isolating cells which express the fusion polypeptide of the invention. Flow cytometric methods such as fluorescence activated cell sorting -(FACS) are particularly useful in this latter application. Preferably, the fusion partners also have protease cleavage sites, such as for Factor Xa or Thrombin, which allow the relevant protease to partially digest the fusion polypeptide of the invention and thereby liberate the recombinant polypeptide of the invention therefrom. The liberated polypeptide can then be isolated from the fusion partner by subsequent chromatographic separation.

Fusion partners according to the invention also include within their scope "epitope tags", which are usually short peptide sequences for which a specific antibody is available. Well known examples of epitope tags for which specific monoclonal antibodies are readily available include c-Myc, influenza virus, haemagglutinin and FLAG tags.

WO 03/060105 PCT/AU02/01768 The step of introducing into the host cell the recombinant polynucleotide may be effected by any suitable method including transfection, and transformation, the choice of which will be dependent on the host cell employed. Such methods are well known to those of skill in the art.

Recombinant polypeptides of the invention may be produced by culturing a host cell transformed with an expression vector containing nucleic acid encoding a polypeptide, biologically active fragment, variant or derivative according to the invention. The conditions appropriate for protein expression will vary with the choice of expression vector and the host cell. This is easily ascertained by one skilled in the art through routine experimentation.

Suitable host cells for expression may be prokaryotic or eukaryotic. One preferred host cell for expression of a polypeptide according to the invention is a bacterium. The bacterium used may be E. coli.

The recombinant protein may be conveniently prepared by a person skilled in the art using standard protocols as for example described in Sambrook, et al., 1989, in particular Sections 16 and 17; Ausubel et al., (1994-1998), in particular Chapters 10 and 16; and Coligan et al., (1995-1997), in particular Chapters 1, 5 and 6.

Alternatively, the AroQ polypeptide, fragments, variants or derivatives may be synthesised using solution synthesis or solid phase synthesis as described, for example, in Chapter 9 of Atherton and Shephard (supra) and in Roberge et al (1995).

6. Antigen-binding molecules The invention also contemplates antigen-binding molecules that bind specifically to the aforementioned polypeptides, fragments, variants and derivatives. Preferably, an antigen-binding molecule according to the invention is interactive with the amino acid sequences set forth in SEQ ID NO: 2 or variants thereof. For example, the antigen-binding molecules may comprise whole polyclonal antibodies. Such antibodies may be prepared, for example, by injecting a polypeptide, fragment, variant or derivative of the invention into a production species, which may include mice or rabbits, to obtain polyclonal antisera.

Methods of producing polyclonal antibodies are well known to those skilled in the art.

Exemplary protocols which may be used are described for example in Coligan et al., CURRENT PROTOCOLS IN IMMUNOLOGY, (John Wiley Sons, Inc, 1991), and Ausubel et al., (1994-1998, supra), in particular Section ll of Chapter 11. In lieu of the WO 03/060105 PCT/AU02/01768 -46polyclonal antisera obtained in the production species, monoclonal antibodies may be produced using the standard method as described, for example, by K5hler and Milstein (1975, Nature 256, 495-497), or by more recent modifications thereof as described, for example, in Coligan et al., (1991, supra) by immortalising spleen or other antibody producing cells derived from a production species which has been inoculated with one or more of the polypeptides, fragments, variants or derivatives of the invention.

The invention also contemplates as antigen-binding molecules Fv, Fab, Fab' and F(ab') 2 immunoglobulin fragments. Alternatively, the antigen-binding molecule may comprise a synthetic stabilised Fv fragment. Exemplary fragments of this type include single chain Fv fragments (sFv, frequently termed scFv) in which a peptide linker is used to bridge the N terminus or C terminus of a VH domain with the C terminus or N-terminus, respectively, of a VL domain. ScFv lack all constant parts of whole antibodies and are not able to activate complement. Suitable peptide linkers for joining the VH and VL domains are those which allow the VH and VL domains to fold into a single polypeptide chain having an antigen binding site with a three dimensional structure similar to that of the antigen binding site of a whole antibody from which the Fv fragment is derived. Linkers having the desired properties may be obtained by the method disclosed in U.S. Patent No 4,946,778. However, in some cases a linker is absent. ScFvs may be prepared, for example, in accordance with methods outlined in Kreber et al (Kreber et al. 1997, J. Immunol.

Methods; 201(1): 35-55). Alternatively, they may be prepared by methods described in U.S. Patent No 5,091,513, European Patent No 239,400 or the articles by Winter and Milstein (1991, Nature 349:293) and Pliickthun et al (1996, In Antibody engineering: A practical approach. 203-252).

Alternatively, the synthetic stabilised Fv fragment comprises a disulphide stabilised Fv (dsFv) in which cysteine residues are introduced into the VH and VL domains such that in the fully folded Fv molecule the two residues will form a disulphide bond therebetween. Suitable methods of producing dsFv are described for example in (Glockscuther et al. Biochem. 29: 1363-1367; Reiter et al. 1994, J. Biol. Chem. 269: 18327-18331; Reiter et al. 1994, Biochem. 33: 5451-5459; Reiter et al. 1994. Cancer Res.

54: 2714-2718; Webber et al. 1995, Mol. Immunol. 32: 249-258).

Also contemplated as antigen-binding molecules are single variable region domains (termed dAbs) as for example disclosed in Ward et al. (1989, Nature 341: 544- WO 03/060105 PCT/AU02/01768 -47- 546); Hamers-Casterman et al. (1993, Nature. 363: 446-448); Davies Riechmann, (1994, FEBSLett. 339: 285-290).

Alternatively, the antigen-binding molecule may comprise a "minibody". In this regard, minibodies are small versions of whole antibodies, which encode in a single chain the essential elements of a whole antibody. Suitably, the minibody is comprised of the VH and VL domains of a native antibody fused to the hinge region and CH3 domain of the immunoglobulin molecule as, for example, disclosed in U.S. Patent No 5,837,821.

In an alternate embodiment, the antigen binding molecule may comprise nonimmunoglobulin derived, protein frameworks. For example, reference may be made to Ku Schultz, (1995, Proc. Natl. Acad. Sci. USA, 92: 652-6556) which discloses a four-helix bundle protein cytochrome b562 having two loops randomised to create complementarity determining regions (CDRs), which have been selected for antigen binding.

The antigen-binding molecule may be multivalent having more than one antigen binding site). Such multivalent molecules may be specific for one or more antigens. Multivalent molecules of this type may be prepared by dimerisation of two antibody fragments through a cysteinyl-containing peptide as, for example disclosed by Adams et al., (1993, Cancer Res. 53: 4026-4034) and Cumber et al. (1992, J. Immunol.

149: 120-126). Alternatively, dimerisation may be facilitated by fusion of the antibody fragments to amphiphilic helices that naturally dimerise (Pack P. Pliinckthun, 1992, Biochem. 31: 1579-1584), or by use of domains (such as the leucine zippers jun and fos) that preferentially heterodimerise (Kostelny et al., 1992, J. Immunol. 148: 1547-1553). In an alternate embodiment, the multivalent molecule may comprise a multivalent single chain antibody (multi-scFv) comprising at least two scFvs linked together by a peptide linker. In this regard, non-covalently or covalently linked scFv dimers termed "diabodies" may be used. Multi-scFvs may be bispecific or greater depending on the number of scFvs employed having different antigen binding specificities. Multi-scFvs may be prepared for example by methods disclosed in U.S. Patent No. 5,892,020.

The antigen-binding molecules of the invention may be used for affinity chromatography in isolating a natural or recombinant polypeptide or biologically active fragment of the invention. For example reference may be made to immunoaffinity chromatographic procedures described in Chapter 9.5 of Coligan et al., (1995-1997, supra).

WO 03/060105 PCT/AU02/01768 -48- The antigen-binding molecules can be used to screen expression libraries for variant polypeptides of the invention as described herein. They can also be used to detect and/or isolate the polypeptides, fragments, variants and derivatives of the invention. Thus, the invention also contemplates the use of antigen-binding molecules to isolate 3dehydroquinase enzymes using for example, any suitable immunoaffinity based method including, but not limited to, immunochromatography and immunoprecipitation. A preferred method utilises solid phase adsorption in which anti-3-dehydroquinase antigenbinding molecules are attached to a suitable resin, the resin is contacted with a sample suspected of containing 3-dehydroquinases, and the 3-dehydroquinases, if any, are subsequently eluted from the resin. Preferred resins include: Sepharose® (Pharmacia), Poros® resins (Roche Molecular Biochemicals, Indianapolis), Actigel SuperflowTM resins (Sterogene Bioseparations Inc., Carlsbad Calif), and DynabeadsTM (Dynal Inc., Lake Success, Alternatively, the antigen-binding molecules can be used to screen for aroQ mutants of Bordetella as a facile means of identifying attenuated Bordetella strains of the pathogenic origin in accordance with the present invention.

7. Immunopotentiating compositions The genetically modified Bordetella strain of the present invention is useful for the design of immunopotentiating compositions that are effective in eliciting an immune response, and preferably a protective immune response, against a pathogenic Bordetella strain, more suitably a natural pathogenic Bordetella counterpart of the genetically modified Bordetella strain, or related organism and will, therefore, find utility in treating and/or preventing whooping cough or related conditions. Thus, the present invention additionally encompasses immunopotentiating compositions comprising one ore more genetically modified Bordetella strains described above in combination with a pharmaceutically acceptable carrier (such as, for example, saline buffer), and optionally in combination with at least one adjuvant such as aluminum hydroxide or a compound belonging to the muramyl peptide family.

Various methods for achieving adjuvant effect for the vaccine include the use of agents such as aluminum hydroxide or phosphate (alum), commonly used as 0.05 to 0.1 percent solution in phosphate buffered saline, admixture with synthetic polymers of sugars (Carbopol) used as 0.25% solution. Other suitable adjuvant compounds can be selected WO 03/060105 PCT/AU02/01768 -49from DDA (dimethyldioctadecyl-ammonium bromide), microparticle poly-lactide-coglycolide (PLG) or nanoparticle PLG formulations (Conway et al., 2001), as well as immune modulating substances, such as lymphokines interferon (IFN) -gamma, interleukin (IL) IL-2 and IL-12) or IFN-gamma inducers compounds, such as poly I:C or other immunostimulatory formulations or compounds including natural products particularly traditional medicines of different ethnobotanical origins.

The immunopotentiating composition according to the present invention may be prepared as an injectable form (either as liquid solution or suspension). However, solid forms suitable for solution in or suspension in, liquid prior to injection may also be prepared. In addition, if desired, the immunopotentiating composition may contain minor amounts of auxiliary substances such as wetting or emulsifying agents, pH buffering agents, or adjuvants which enhance the effectiveness of the vaccines.

The immunopotentiating compositions of the invention are administered in a manner compatible with the dosage formulation, and in such amount as will be therapeutically effective and immunogenic. The quantity to be administered depends on the subject to be treated including, the capacity of the individual's immune system to induce an immune response.

In a preferred embodiment, the immunopotentiating composition is formulated for intranasal and/or inhalational administration. The genetically modified Bordetella strainscontaining compositions of the present invention are preferably used as immunogenic agents for the treatment and/or prevention of diseases associated with infection by pathogenic strains of Bordetella and related organisms. Thus, such compositions are preferably delivered to the lower and middle respiratory tracts by an inhalation route. For administration by inhalation into the lower respiratory tract the bronchioles), the genetically modified Bordetella can be formulated into a solution and/or a suspension of particles in a carrier appropriate for inhalation. Such carriers are also well known to the ordinary artisan familiar with inhalants for the delivery of fine droplets and insufflations and for the delivery of inhalable fine particles. The mean particle size of the droplet or powder for inhalation administration into the lower respiratory tract the bronchioles) is commonly, but not exclusively, in the order of from about 0.5 to about 10 microns, and typically ranges from about 0.5 to about 1.0 micron, comprised of powders, mists or aerosols, into the lower respiratory tract (Remington's, Id., at page 1451). For inhalation administration into the middle respiratory tract the oro-pharynx) the mean particle WO 03/060105 PCT/AU02/01768 size of the droplet or powder is commonly, but not exclusively, in the order of from about 1 to about 50 microns, and typically from about 1 to about 10 micron. The mean particle size of the droplet or powder for inhalation administration into the upper respiratory tract the nasal mucosa) is commonly, but not exclusively, in the order of from about 10 to 355 microns, and typically from about 20 to about 200 micron. In one embodiment for delivery of an active to the middle and lower respiratory tract, the mean particle size of the droplet or powder is preferably in the range of from about 0.8 to 1.2 microns. In determining aerosol compositions, reference may also be made, for example, to a text entitled "Pharmaceutical Inhalation Aerosol Technology" edited by A. J. Hickey and published by Marcel Dekker, which discusses the importance of.particle size in therapeutic aerosols for effective delivery of a medicament to a destination site.

Compositions in the form of droplets, mists or aerosols typically comprise surfactants to ensure good dispersion of a powdered medicament and also to provide for smooth operation of the valve through which the composition is dispensed. Conventional surfactants include, but are not restricted to, sorbitan triolate and oleic acid. Solvents have also been used to increase the solubility of the surfactant in the propellant.

For inhalation of droplets, mists and aerosols various devices such as nebulisers or pressurised aerosol generators are readily available. In addition, such devices can be metered to provide uniformity of dosing (Remington's, Chlorofluorocarbons (CFC) can be used as propellants but other propellants that are more environmentally friendly such as hydrofluorocarbon (HFC) propellants could also be employed and in this regard reference may be made to various HFC propellants disclosed in International Publications W092/08447, W092/06675 and W091/04011.

When providing a patient with live bacterial immonpotentiating compositions or vaccines, the dosage of the composition will depend upon the route of administration and will vary according to the age of the patient to be vaccinated and, to a lesser degree, the size, weight, height, sex, general medical condition, previous medical history etc of the person to be vaccinated as well as the excipient or adjuvant present in the composition. In general, it is desirable to provide the recipient with a dosage range of from about 104 colony forming units (cfu) to 10 1 cfu, more suitably of from about 105 cfu to 01 0 cfu although a lower or higher dosage may be administered. Most preferably, the vaccine composition according to the present invention is administered via an intranasal and/or inhalational route and as part of a single or multiple administrations.

WO 03/060105 PCT/AU02/01768 -51 In the case of neonates, the dose will be approximately four times less than for an adult, and in the case of young children (4-6 years old), the dose will be approximately half the dose used for an adult healthy patient.

In some instances, it will be necessary to proceed with multiple administrations of the composition according to the present invention, usually not exceeding six administrations, more usually not exceeding four administrations, and preferably one or more, usually at least about three administrations. The administrations will normally be at from two to twclve week intervals, more usually from three to five week intervals. Periodic boosters at intervals of 1-5 years, usually three years, will be desirable to maintain the desired levels of protective immunity.

8. Dendritic cell embodiments The invention further provides a composition of matter for eliciting a cellular and/or a humoral immune response against a target antigen, comprising dendritic cells which have been exposed to a genetically modified Bordetella strain as broadly described above for a time and under conditions sufficient to express a processed or modified antigen derived from said Bordetella strain for presentation to, and modulation of, T cells.

Bordetella antigen-primed dendritic cells may be prepared by a method including contacting dendritic cells with a genetically modified Bordetella as broadly described above, for a time and under conditions sufficient to permit said Bordetella to be internalised by the dendritic cells; and culturing the Bordetella antigen-containing dendritic cells for a time and under conditions sufficient for the modified antigen to be processed for presentation by the dendritic cells.

8.1 Sources of dendritic cells The dendritic cells used in this invention can be isolated by methods known to those of skill in the art. They can be autologous or allogeneic with respect to the subject to be treated. Suitably, mammalian and preferably human dendritic cells are used from an appropriate tissue source, which is suitably blood or bone marrow. Dendritic cell precursors, from which the immature dendritic cells for use in antigen intemrnalisation according to the invention, are present in blood as peripheral blood mononuclear cells (PBMCs). Although most easily obtainable from blood, the precursor cells may also be obtained from any tissue in which they reside, including bone marrow and spleen tissue.

Peripheral blood precursors may be purified using monoclonal antibodies, density WO 03/060105 PCT/AU02/01768 -52gradients or centrifugation or any combination of these. Circulating frequency may be increased in vivo using flt-3 ligand. When cultured in the presence of cytokines such as a combination of GM-CSF and IL-4 or IL-13 as described below, the non-proliferating precursor cells give rise to immature dendritic cells for use in this invention.

An exemplary method for culturing pluripotential PBMCs to produce immature dendritic cells is described by Albert et al. (International Publication WO 99/42564). In this respect, cultures of immature dendritic cells, ie. antigen-capturing phagocytic dendritic cells, may be obtained by culturing non-proliferating precursor cells (PBMCs) in the presence of cytokines which promote their differentiation. A combination of GM-CSF and IL-4 produces significant quantities of the immature dendritic cells, ie. antigen-capturing phagocytic or interalisation-competent dendritic cells. Other cytokines that promote differentiation of precursor cells into immature dendritic cells include, but are not limited to, IL-13.

Maturation of dendritic cells requires the addition to the cell environment, preferably the culture medium, of a dendritic cell one or more maturation factors which may be selected from monocyte conditioned medium and/or factors including TNF-a, IL-6, IFN-a and IL-1. Alternatively, a mixture of necrotic cells or necrotic cell lysate may be added to induce maturation. Maturation can be induced in vitro using plastic adherence, cytokines, LPS, bacteria, DNA containing CpG repeats, RNA or polyIC, necrotic cells. In this regard, reference may be made to Steinman et al. (International Publication WO 97/29182) who describe methods and compositions for isolation and maturation of dendritic cells.

Other methods for isolation, expansion and/or maturation of dendritic cells for the purpose of the present invention are described for example by Takamizawa et al. (1997, J Immunol, 158 2134-2142), Thomas and Lipsky (1994, J Immunol, 153 4016- 4028), O'Doherty et al. (1994, Immunology, 82 487-93), Feamley et al. (1997, Blood, 89 3708-3716), Weissman et al. (1995, Proc Natl Acad Sci US A, 92 826-830), Freudenthal and Steinman (1990, Proc Natl Acad Sci USA, 87 7698-7702), Romani et al. (1996, JImmunol Methods, 196 137-151), Reddy et al. (1997, Blood, 90 3640-3646), Thumher et al. (1997, Exp Hematol, 25 232-237), Caux et al. (1996, J Exp Med, 184 695-706; 1996, Blood, 87 2376-85), Luft et al. (1998, Exp Hematol, 26 489-500; 1998, JImmunol, 161 1947-1953), Cella et al. (1999, JExp Med, 189 821-829; 1997, Nature, 388 (644): 782-787; 1996, J Exp Med, 184 747-572), WO 03/060105 PCT/AU02/01768 -53- Ahonen et al. (1999, Cell Immunol, 197(1): 62-72) and Piemonti et al. (1999, JImmunol, 162 6473-6481).

Alternatively, transformed or immortalised dendritic cell lines may be produced using oncogenes such as v-myc as for example described by Paglia et al. (1993, JExp Med, 178 1893-1901).

8.2 Antigen priming of dendritic cells The number of a genetically modified Bordetella cells to be placed in contact with dendritic cells can be determined empirically by persons of skill in the art. Dendritic cells are incubated with Bordetella cells for 1-2 hr at 370 C. For most antigens, 10 tg/mL to 1- 10 million dendritic cells is suitable for priming the dendritic cells. In a preferred embodiment, immature dendritic cells are utilised for the antigen internalisation.

The genetically modified Bordetella cells should be exposed to the dendritic cells for a period of time sufficient for the dendritic cells to internalise the antigen. The time necessary for the cells to internalise and present the processed Bordetella antigens may be determined using pulse-chase protocols in which exposure to Bordetella cells is followed by a washout period. Once the minimum time necessary for cells to express processed Bordetella antigen on their surface is determined, a protocol may be used to prepare cells and Bordetella cells for eliciting immunogenic responses. Those of skill in the art will recognise in this regard that the length of time necessary for an antigen-presenting cell to phagocytose or internalise an antigen may vary depending on the antigen used. Efficiency of priming of the dendritic cells can be determined by assaying T cell cytolytic activity in vitro or using dendritic cells as targets of CTLs. Other methods known to practitioners in the art, which can detect the presence of antigen on the surface of dendritic cells after exposure to the Bordetella cells, are also contemplated by the presented invention.

The primed dendritic cells of the present invention have the capacity to efficiently present a processed Bordetella antigen in the form of peptides on both MHC class I and class II molecules. Modified antigens are acquired by dendritic cells through the exogenous pathway by phagocytosis and, as a result, efficiently charge MHC class II molecules. Accordingly, both CD4 T helper lymphocytes and CTL may be activated by dendritic cells presenting Bordetella antigen in the context of MHC class II. These lymphocytes can provide critical sources of help, both for generating active CD8 CTL and can in some circumstances be primed as CD4 CTL with specificity for the target antigen WO 03/060105 PCT/AU02/01768 -54during the acute response to antigen, and for generating the memory that is required for long term resistance and vaccination. Further, Bordetella cell uptake and presentation by dendritic cells, allows these cells to tailor the peptides that are appropriate for an individual's MHC products, and increases the number of specialised stimulatory antigenpresenting cells. Moreover, dendritic cells can be charged with multiple antigens on multiple MHCs to yield polyclonal or oligoclonal stimulation ofT cells. Thus, by using the modified antigens of the present invention to charge MHC class I and class II molecules, efficient T cell modulation in situ can be achieved.

8.3 Therapeutic and prophylactic applications of Bordetella-primed dendritic cells Genetically modified Bordetella-primed dendritic cells and antigen-specific T lymphocytes generated with these dendritic cells infra can be used as actives in immunomodulating compositions for prophylactic or therapeutic applications. The primed cells, which are preferably mature dendritic cells, can be injected by any method that elicits an immune response into a syngeneic animal or human. Preferably, dendritic cells are injected back into the same animal or human from whom the source cells were obtained, and are, therefore, autologous dendritic cells. The injection site may be subcutaneous, intraperitoneal, intramuscular, intradermal, or intravenous. The number of conjugateprimed dendritic cells reinjected back into the animal or human in need of treatment may vary depending on inter alia, the virulence of the Bordetella cells and size of the individual. This number may range for example between about 104 and 108, and more preferably between about 106 and 10 7 conjugate-primed dendritic cells. The dendritic cells should be administered in a pharmaceutically acceptable carrier, which is non-toxic to the cells and the individual. Such carrier may be the growth medium in which the dendritic cells were grown, or any suitable buffering medium such as phosphate buffered saline.

A key feature in the function of dendritic cells in situ is the capacity to migrate or home to the T-dependent regions of lymphoid tissues, where the dendritic cells would be in an optimal position to select the requisite antigen-reactive T cells from the pool of recirculating quiescent lymphocytes and thereby initiate the T-dependent response.

In a preferred embodiment, the Bordetella-primed dendritic cells of the invention are suitable for treatment or prophylaxis of infection by a strain, preferably a pathogenic strain, of Bordetella. In another embodiment, the Bordetella-primed dendritic cells of the invention could also be used for generating large numbers of CD8 or CD4+ CTL, for WO 03/060105 PCT/AU02/01768 adoptive transfer to immunosuppressed individuals who are unable to mount normal immune responses. For example, antigen-specific CD8 CTL can be adoptively transferred for therapeutic purposes in individuals afflicted with HIV infection (Koup et al., 1991, J Exp. Med., 174: 1593-1600; Carmichael et al., 1993, J. Exp. Med., 177: 249-256; and Johnson et al., 1992, J Exp. Med., 175: 961-971), malaria (Hill et al., 1992, Nature, 360: 434-439) and malignant tumours such as melanoma (Van der Brogen et al., 1991, Science, 254: 1643-1647; and Young and Steinman, 1990, J. Exp. Med., 171: 1315-1332).

In yet another embodiment, the genetically modified Bordetella is used as a delivery vehicle for heterologous antigens as described above and thus the resulting Bordetella-primed dendritic cells of the invention may be useful inter alia for treatment or prophylaxis of a viral, bacterial or parasitic infection or for treatment of a condition such as cancer. Viral infections contemplated by the present invention include, but are not restricted to, infections caused by HIV, Hepatitis, Influenza, Japanese encephalitis virus, Epstein-Barr virus and respiratory syncytial virus. Bacterial infections include, but are not restricted to, those caused by Neisseria species, Meningococcal species, Haemophilus species Salmonella species, Streptococcal species, Legionella species and Mycobacterium species. Parasitic infections encompassed by the invention include, but are not restricted to, those caused by Plasmodium species, Schistosoma species, Leishmania species, Trypanosoma species, Toxoplasma species and Giardia species. Cancers which could be suitably treated in accordance with the practices of this invention include cancers of the lung, breast, ovary, cervix, colon, head and neck, pancreas, prostate, stomach, bladder, kidney, bone liver, oesophagus, brain, testicle, uterus, melanoma and the various leukemias and lymphomas.

The effectiveness of the immunisation may be assessed using any suitable technique. For example, CTL lysis assays may be employed using stimulated splenocytes or peripheral blood mononuclear cells (PBMC) on peptide coated or recombinant virus infected cells using 5 1 Cr labelled target cells. Such assays can be performed using for example primate, mouse or human cells (Allen et al., 2000, J. Immunol. 164(9): 4968-4978 also Woodberry et al., infra). Alternatively, the efficacy of the immunisation may be monitored using one or more techniques including, but not limited to, HLA class I tetramer staining of both fresh and stimulated PBMCs (see for example Allen et al., supra), proliferation assays (Allen et al., supra), ELISPOT assays and intracellular IFN-gamma WO 03/060105 PCT/AU02/01768 -56staining (Allen et al., supra), ELISA Assays for linear B cell responses; and Western blots of cell sample expressing the synthetic polynucleotides In order that the invention may be readily understood and put into practical effect, particular preferred embodiments will now be described by way of the following nonlimiting examples.

WO 03/060105 PCT/AU02/01768 -57-

EXAMPLES

EXAMPLE 1 Bacterial strains, plasmids, media and growth conditions A complete list of bacterial strains and plasmids used in this study is provided in Table 1. E. coli strains were routinely cultured in LB broth or on LB agar (Oxoid) overnight at 370 C. B. pertussis was routinely grown in a modified version of Verwey liquid medium (Farrell 2000 or Stainer Scholte medium (Stainer Scholte 1971) and on Bordet-Gengou (BG) agar (Becton Dickinson) containing 15% sterile-defibrinated sheep blood for 2-3 days at 35-370 C. Cohen-Wheeler (CW) agar (Cohen Wheeler 1946) containing 10% sterile-defibrinated sheep blood and 10 mM MgCl 2 was used for B.

pertussis growth during conjugation experiments.

TABLE 1 Bacterial Strains and plasmids Strains Relevant properties Source or reference Bacteria E.coli Mobilising strain, recA::RP4-2-Tc::Mu Roberts et al. (1990) JM101 Cloning host Cloning host 583/90 Cloning host, aroD mutant B. pertussis ATCC9340 vir Rosetti 1997 BP304 Tohama I spontaneous mutant, SmR, vir Tohama I vir 1950's Japan/ vaccine Plasmids pNEB193 Cloning vector, Ap

R

LacZ New England Biolabs, Inc.

pUC4K Source of KmR cassette Pharmacia Biotech pRTP1 Ap

R

rpsL, oriT, cos Stibitz, Black Falkow (1986) WO 03/060105 PCT/AU02/01768 -58- Cyclodextrin solid media (Imaizumi et al. 1983) was modified by removing the casamino acid component and increasing the amount of monosodium glutamate to 17 g (Frohlich et al. 1995). This modified CSM together with M9 minimal agar (Difco), with or without aromatic compounds (aromix), was used to test for the aro- phenotype of B.

pertussis or E. coli mutants respectively. The final concentrations of aromix consisted of pg/mL each of tryptophan, tyrosine and phenylalanine; and 10 ,tg/mL each of dihydroxybenzoic acid and para-aminobenzoic acid.

All antibiotic concentrations used for selection were as follows: kanamycin- Vig/mL, streptomycin-200 itg/mL and ampicillin- 100 pg/mL.

EXAMPLE 2 Preparation and manipulation ofDNA Extraction ofplasmid DNA from E. coli strains or agarose gels was accomplished using a Prep-A-Gene DNA purification Kit (BioRad). Genomic DNA (gDNA) was purified from B. pertussis using a BioRad Genomic DNA Isolation Kit. All DNA manipulations were carried out using the protocols described elsewhere (Sambrook et al., 1989). Restriction endonucleases, T4 DNA ligase and alkaline phosphatase were purchased from either MBI Fermentas, New England Biolabs or Amersham Pharmacia Biotech and were used according to the manufacturers recommendations. The PCR kit used was purchased from Fisher Biotec.

EXAMPLE 3 Characterisation of the aroQ gene To detect whether B. pertussis possessed the aroD gene from the aromatic biosynthetic pathway, its genomic library was electroporated into the aroD mutant, E. coli 583/90. During these attempts an isolate was found which restored the mutant to wild type E. coli and allowed it to grow on media lacking aromatic compounds. It was assumed that the plasmid rescuing the isolate contained the aroD gene.

Restriction digestion and electrophoresis were used to determine an insert size of Kb. To facilitate sequencing, the plasmid was further digested to produce a smaller 1 Kb fragment. This plasmid was subsequently named pUSQBord4 (Figure Analysis of WO 03/060105 PCT/AU02/01768 -59the pUSQBord4 insert sequence revealed that the approximate 500 bp gene was not similar to the consensus sequence of the aroD gene. A BLAST search using ANGIS showed that B. pertussis possessed the aroQ gene similar to the catabolic pathway of the fungus, Aspergillus nidulans. The aroQ genes from several other microorganisms were used in the ANGIS PILEUP program to confirm the identity of this gene (Figure 2).

Methods Cloning of the aroO gene To create a gene library, the B. pertussis chromosomal DNA was partially digested with the Sau3AI restriction endonuclease. Ligation was attempted as described elsewhere (Sambrook et al., 1989) with the different sized gDNA fragments and BamHIcleaved pNEB 193 which had been previously alkaline phosphatased.

The ligation mix was electroporated into the aroD E. coli 583/90 by electroporation using 2.5 kV and 2 mm gap cuvettes to yield a desired pulse length of 6 ms (BTX ECM 600 Electrocell manipulator, Novex Australia). M9 minimal agar plates supplemented with ampicillin (100 atg/ml) were used to select for transformants.

DNA sequencing DNA sequencing service was provided by the Australian Genome Research Facility (Ritchie Laboratories, University of Queensland). Sequences were then analysed at the University of Southern Queensland using the ANGIS (Australian National Genome Information Service) program. Programs used within this site were the Blast, Mapping and Pileup programs.

EXAMPLE 4 Generation of a mutation in the aroO gene The cloned aroQ gene was inactivated in vitro by double digesting the plasmid with the restriction enzymes NgoMIV and BssHI to remove a large portion of the gene (300 bp) as well as two BssHI-BssHI fragments (719 bp and 44 bp see Figure A kanamycin resistance cassette derived from pUC4K was amplified by the polymerase chain reaction (PCR) using primers with NgoMIV and BssHI restriction ends to create a fragment size of approximately 940 bp. This NgoMIV-BssHI cassette was then ligated into pUSQBord4 to replace the gene portion removed by digestion. This new plasmid was WO 03/060105 PCT/AU02/01768 named pUSQBord7. The 719 bp BssHI-BssHI fragment was then re-ligated back into the plasmid and subsequently re-named pUSQBord8 after confirmation of the correct orientation of the fragment. This plasmid was then digested with EcoRI and BamHI to break it into its original components the insert and pNEB193) so that the EcoRI- BamHI insert could be moved into a suicide vector.

In order to move the inactivated aroQ gene into B. pertussis, a suicide vector and mobilising strain were required. pRTP1 is mobilisable due to trans-acting factors encoded by chromosomal RP4 sequences present in E. coli SM1Opir pRTP1 replicates in this strain due to the presence of the pir gene product carried on a lysogenic phage (Roberts et al. 1990). The lack of this gene in B. pertussis provides the ideal conditions necessary for plasmid suicide, following conjugation. The gene for the E. coli S12 ribosomal protein is also encoded by the plasmid, which renders the Streptomycin resistant (SmR) host Sm sensative (Stibitz et al. 1986). The EcoRI-BamHI insert mentioned above was ligated directly into pRTP1 to produce third plasmid pUSQBord9.

To increase the chance of a recombinant event occurring it was necessary to add a scaffold sequence from the original 1.5 Kb fragment, upstream from the inactivated aroQ locus (located in pUSQBordl- not shown). A triple fragment ligation was performed (Figure 3) to produce a new shuttle vector ready for conjugation. Following electroporation of the complete plasmid into E. coli SMlOXpir, kanamycin resistant (KmR) and ampicillin resistant (ApR) transconjugants were isolated on LB agar with appropriate antibiotics.

This new plasmid called pUSQBordlO was used to transform E. coli 583/90 to check whether the aroQ gene had been inactivated. Results showed that transformants could not grow without supplements of aromix, indicating that the gene was inactive.

E. coli SMlOXpir harbouring pUSQBordlO was conjugated with B. pertussis BP304 on CW agar and transconjugants were selected on CW agar containing kanamycin and streptomycin. It was assumed that B. pertussis transconjugates exhibiting KmR had incorporated the mutated aroQ gene into their genome. This should have occurred due to a crossover involving the remaining aroQ sequence flanking the Km R gene.

To verify that only the donor fragment has been incorporated and not the entire plasmid, KmR isolates were subcultured onto CW agar with streptomycin to again check for SmR. If vector integration had occurred via a single crossover, then the S12 allele on pRTP1 renders the isolates Sm' (Roberts et al. 1990).

WO 03/060105 PCT/AU02/01768 -61- Methods PCR methods For the kanamycin resistance cassette PCR, 2 pL of template DNA was added to 48 pL of master mixture (made immediately upon use) containing 5 pL of 10X PCR buffer; 10 pL of dNTPs h(2 mM); 2 /L MgCl 2 (50 mM); 20 pmoles (each) of primers FORKAN and BACKAN as set forth in Table 2; and 2.5 U of Taq polymerase.

Amplification was performed in a programmable thermal controller (PTC-100, MJ Research inc.) with the following parameters: 5 min at 940 C followed by 5 initial cycles of s at 350 C, 1 min at 720 C, and 30 s at 940 C; and 30 cycles of 30 s at 550 C, 1 min at 72° C, and 30 s at 94° C.

TABLE 2 PCR Primers Primer Name 5' 3' Primer Sequence FORKAN GTGCCGGCGTGAATCTCTGATGTTACATTG BACKAN GGGCGCGCACTAGTGTTACAACCAATTAAC FORQ ATGGCGCAACGCATTCTTGT BACQ2 GTTTTGAGTTTTCGGAGGTC Bacterial conjugation The method described by Roberts et al. (1990) was used for the conjugal transfer of DNA from E.coli to B. pertussis. Briefly, after 72 hours growth B. pertussis BP304 was swept from BG agar specifically using a sterile dacron swab and resuspended in phosphate buffered saline (PBS). The optical density of the suspension at 630 nm was determined and adjusted to an absorbance of 0.4. This procedure was repeated using E. coli SMlOpir containing pUSQBordlO (see results section) except that growth was swept from LB agar supplemented with kanamycin.

A mixture of the above suspensions was made at a ratio of 10:1 and 100:1 B.

pertussis to E.coli cells. These were placed onto sterile nitrocellulose resting on a foundation of CW agar and incubated at 370 C for 4 hours. Following incubation, growth was again swept from each piece of nitrocellulose and resuspended as above. Final WO 03/060105 PCT/AU02/01768 -62suspensions of bacteria were plated onto CW agar supplemented with kanamycin, streptomycin and aromix. Plates were incubated for up to 5 days at 35-370 C.

EXAMPLE Confirmation of the transconjugants for aromatic-dependence Transconjugants displaying both KmR and SmR were verified using CW agar plates with and without aromix. B. pertussis aroQ mutant colonies grown on medium with aromix were found to have a slower growth rate than that of the wild-type. Mutant colonies failed to grow without supplements of aromix.

To investigate the genotypic properties of the aroQ mutant, PCR incorporating aroQ gene primers was used to amplify a fragment containing the aroQ gene with an approximate size of 500 bp in the parental strain. It can be seen in Figure 4 that the B.

pertussis aroQ mutant has a fragment size of 1150 bp This increase in fragment size approximately corresponds to the size of the aroQ fragment (500 bp) minus the 300 bp deletion plus the insertion of the 940 bp KmR cassette. To confirm this result, the 1150 bp fragment was extracted from an agarose gel and PCR with KmR gene primers was found to amplify the KmR gene (results not shown).

Methods PCR methods For the aroQ PCR, 1 /L of template DNA was added to 49 pL of master mix containing 5 iL of 10X PCR buffer; 5 uL of dNTPs (2 mM);1.5 pL MgCl 2 (50 mM); pmoles (each) of primers FORQ and BACQ2 as set forth in Table 2, supra; 5 IL 10X PCR Enhancer (Gibco BRL); and 2.5 U of Taq polymerase. Amplification was performed with the following parameters: 1 min at 950 C followed by 30 cycles of 45 s at 950 C, 30 s at 42' C and 1 min at 68 0

C.

EXAMPLE 6 Determination of the presence or production of the major virulence factors by the aroQ mutant ofB. pertussis Experiments carried out to determine the presence of the major virulence determinants including FHA, PT and pertactin, also included in the currently marketed WO 03/060105 PCT/AU02/01768 -63acellular vaccine (PT is detoxified), in the aroQ B. pertussis revealed that all the above factors were present in or generated by the aroQ mutant vaccine strain (Table 3).

TABLE 3 Screening for the presence ofFHA, PT and the 69 kD pertactin in the aroQ B. pertussis by western blotting using specific monoclonal antibodies Fraction tested Supernatant Bacterial pellet FHA PT Pertactin EX4MPLE 7 Safety of the aroQ mutant in vivo in mice Mice were inoculated with 6 X 107, 6 X 108 or 6 X 109 CFUs of the aroQ mutant microorganisms by the intranasal route and observed over a period of 3 weeks for any morbidity or mortality. None of the vaccinated mice were found to suffer any ill effects (Figures 5A and 5B). In comparison, in mice inoculated with 9.3 X 10 6 CFU of virulent B.

pertussis, the virulent microorganisms were detectable in high numbers in the lungs of infected mice (Figure Method Determination of safety In the first experiment, a total of 15 mice were inoculated with 6x10 7 6xl08 or 9 colony-forming units (CFUs) of the aroQ mutant microorganisms by the intranasal route and observed over a period of 4 weeks for any morbidity or mortality. In the second experiment, 24 mice were vaccinated with 8x108 CFU mutant microorganisms and their clearance from the lungs monitored at 1, 3, 5, 7, 14 and 21 days post-vaccination by the intranasal route. In another experiment, samples were taken at 2, 4, 6, 8, 10, 12 and 14 day intervals. Sera were also collected from mice in the second experiment for determination of antibodies and interleukin 12 (IL-12) levels.

WO 03/060105 PCT/AU02/01768 -64- EX4MPLE 8 Persistence of the aroQ B. pertussis mutant in mice Two experiments were carried out. B. pertussis strains were grown on BG plates for 24-72 hrs, containing aromix when required. The bacteria were resuspended in PBS containing 1% casamino acids (in vivo grade). An absorbance of 0.1 at 630 nm was found to correspond to approximately 1 X 10 9 colony-forming units (CFU) of the B. pertussis aroQ mutant and 3 X 10 9 CFU ofB. pertussis Tohama I. Ten-fold serial dilutions in PBS 1% casamino acids were made to prepare vaccine and challenge doses. Dilutions were spotted onto BG plates using the standard Miles and Misra method to confirm the number ofCFU.

Female 6-8 week old Balb/c mice were mildly sedated with xylazil (0.04 mL) and piL of the bacterial suspension was placed onto the external nares of each mouse using a micropipette whereby the transfer from the nares to the respiratory tract of was achieved via inhalation by the mouse. At specific time intervals, depending upon the type of experiments, groups of mice were sacrificed and the trachea, lungs and spleen aseptically removed. The trachea and lungs were homogenised in 0.5ml PBS 1% casamino acids.

serial dilutions of each homogenate were plated onto BG agar (with aromix when required) using the standard Miles and Misra method and the number of CFU was determined after 2 days incubation at 370 C.

The results presented in Figures 5A and B show that the aroQ mutant was detectable in the lungs of mice up to day 11 post-administration by the intranasal route.

EXAMPLE 9 The potential protective capacity of the aroQ B. pertussis mutant in mice The potential protective capacity of the aroQ mutant vaccine was assessed in mice, which had received 3 doses of the vaccine, by determining the rate of clearance of a dose (2.0x10 8 CFU of B. pertussis Tohama 1 strain from the lungs.

Method and Results Experimental design for vaccination and challenge A group of mice was vaccinated with either three doses of the mutant aroQ vaccine strain (1x10l, 5x10 8 and 1x10 9 CFU) or the placebo solvent at days 1, 7 and 14 by WO 03/060105 PCT/AU02/01768 the intranasal route. All mice were challenged on day 28 with virulent B. pertussis Tohama I by the intranasal route. It was discovered that the vaccinated mice had cleared the virulent microorganisms by day 6 post-challenge (Figure On the other hand, the virulent microorganisms were isolated from the lung homogenates of placebo vaccinated group of mice until day 30 post-challenge (Figure Sera and lung homogenates from these mice were collected for determination of total antibody levels and isotypes. Antigens used were killed whole cells of B. pertussis in the Indirect ELISA or purified FHA or PT in the Western dot blotting assays. Serum IL-12 levels were determined using commercial sandwich ELISA kits (Biosource). Determination of IL-2, IL-5 and INF-y in splenocyte culture supematants obtained after stimulation with either FHA or chemically-inactivated pertussis toxin (PTxoid) were also carried out using commercially available sandwich ELISA kits (Biosource).

EXAMPLE Serum antibody and IL-12 response of mice vaccinated with the aroO B. pertussis in mice Analysis of sera from the safety experiment described in Example 7 revealed that all mice immunised with the aroQ B, pertussis mutant developed antibodies against FHA and PT (Table TABLE FHA- and PT-specific serum antibody titres in mice immunised with the aroQ B. pertussis mutant Antibody titre against Day post-immunisation FHA PT 1 >50 ND* 3 >50 ND* >50 ND* 7 >50 14 >50 ND*: Not detectable at a dilution of 1/50 of the serum sample WO 03/060105 PCT/AU02/01768 -66- However anti-PT antibody titre was greater than 50 on days 5 and 7 postvaccination but not detectable at that dilution of the serum on days 1, 3 and 5 postimmunisation and as such probably less than 50. There was also an induction of a serum IL-12 response beginning day 3 and reaching a reasonably high value at day 5 postimmunisation (data not shown). It is clear, however, that three vaccine doses are required to generate a significant anti-B. pertussis IgA and IgG in the lung homogenates although vaccination with one dose was sufficient to initiate the induction of the serum IgG response (Table 6A).

TABLE 6A Antibody titres against whole-cell B. pertussis Tohama I in pooled mouse samples Schedule: Lung homogenates Serum IgA IgG IgA IgG Polyvalent 7 days post 1 vaccination 0 0 0 11 7 days post 2 vaccinations 0 0 60 167 125 7 days post 3 vaccinations 240 52 80 650 600 14 days post 3 vaccinations 640 84 80 779 1280 21 days post 3 vaccinations 960 974 800 >1067 1625 28 days post 3 vaccinations 320 ND 460 >1067 >2560 days post 3 vaccinations 2080 ND 320 >1067 825 Values represent reciprocal end point titres ND not determined It can be seen from the Table 6A that the titre of the antibodies of the IgA isotype was significant in mice immunised with three doses of the attenuated vaccine postchallenge with virulent B. pertussis.

WO 03/060105 PCT/AU02/01768 -67- TABLE 6B Antibody titres against whole-cell B. pertussis Tohama I in pooled samples from control and vaccinated mice post-challenge Lung homogenates Serum IgA IgG IgA IgG Days post control vaccinated control vaccinated control vaccinated control vaccinated Challenge mice mice mice mice mice mice mice mice -1 0 >1200 0 990 0 29 0 >960 2 0 1200 0 2550 0 21 0 >960 4 0 1200 0 1190 0 253 0 >640 6 0 >1200 0 >1270 0 213 0 >640 8 0 >1200 20 >1270 0 253 0 >640 1200 >1200 70 >1270 17 157 133 >640 12 ND ND ND ND 101 79 160 >640 14 1200 >1200 290 >1270 77 93 >960 >640 16 1200 >1200 630 >1270 29 65 >960 >640 21 >1200 >1200 630 >1060 37 39 >960 >640 >1200 >1200 750 >1270 37 237 >960 >640 -1 pre-challenge Values represent reciprocal end point titres There was no antibody response in control mice post-challenge until day 8 postchallenge (Table 6B). However the IgA antibody titres in the lung homogenates versus the serum IgA titres of vaccinated mice were significantly greater at all intervals postchallenge. Furthermore the IgG antibody levels in the lung homogenates could be considered to be at least at the same level, if not greater, than its serum IgG antibody counterpart.

On the other hand, mice vaccinated with an acellular vaccine (DTaP; InfantrixTM) showed no IgA production with only small amount of IgG in the lung homogenates but with significant amounts of IgG in the serum at day 28 post-immunisation with 1/ 1 0 th the human dose at day 1 and 21 (data not shown).

WO 03/060105 PCT/AU02/01768 -68- Method Determination of antibody and interleukin 12 (IL-12) levels in the sera of vaccinated mice Antibody titres of sera against B. pertussis were determined against the 2 most significant virulence factors, purified FHA and PT, in dot blot assays using the procedure described by Towbin et al. (1979) except that no SDS-PAGE (sodium dodecyl polyacrylamide gel electrophoresis) was carried out. Determination of IL-12 was carried out using a commercial kit (BioSource, Australia). The level of IL-12 was accomplished using commercial kit (Biosource, Australia). The anti-IgG and IgA antibody titres against killed whole B pertussis cells were determined using a standard indirect ELISA assay.

Determination of the presence or production of FHA, PT or Pertactin Determination of the production of FHA, PT and pertactin by the mutant strain was carried out using specific monoclonal antibodies by western blotting essentially as described by Towbin et al (1979). Briefly the aroQ mutant of B. pertussis was grown in Stainer and Scholte medium for a period of 48-72 hours and subjected to centrifugation at 4000xg for 10 mins. The supernatant concentrated by pervaporation and the lysed bacterial sediment subjected to SDS-PAGE followed by western blotting. The blot was developed using monoclonal antibodies against FHA, PT or pertactin (obtained through the courtesy of CSL Ltd, Parkville).

EXAMPLE 11 Determination of the interleukins IL-2 and INF-y in the antigen stimulated splenocyte culture supernatants Both the interleukin-2 and interferon-7 (IFN-7) titres were determined using a Cytoscreen Mouse IFN-7 kit (Biosource International, Camarillo, CA). The procedure was conducted as per instructions. A standard curve was plotted from the standard data, and interleukin concentrations for the splenocyte supernatants calculated. The antigens used for stimulation of the splenocyte cultures were chemically-inactivated pertussis toxoid or pure filamentous hamagglutinin (FHA).

The results presented in Figures 7 and 8 were obtained from pooled spleen samples from both the control and vaccinated mice pre- and post-challenge. There was significant production of IL-2 following challenge at days 10, 14, 21 and day 30 postchallenge with PTxoid. There was also a significant production of IL-2 at days 10 and WO 03/060105 PCT/AU02/01768 -69post-challenge with FHA. The low response at day 14 post-challenge was not expected and as such it is considered to be an operator error. There was significant production of INF-y in the vaccinated mice pre- and post-challenge, indicating the induction and promotion of Thl type responses considered to be responsible for long term protection (see Figure 8).

DISCUSSION OF THE EXAMPLES During an attempt to clone the aroD gene from the B. pertussis genome, a fragment containing a gene with amino acid sequence homology to the aroQ-encoded 3-3dehydroquinase enzyme of Actinobacillus pleuropneumoniae (Lalonde et al. 1994), reported previously to have homology with the eukaryotic genes in the quinic acid catabolic pathway ofAspergillus nidulans, was discovered (Rosetti 1997). Even though the sequence of the gene indicated its involvement in the catabolic pathway, the fact that it rescued the E. coli aroD mutant suggested that it was capable of functioning in the aromatic amino acid biosynthetic pathway. PCR with aroD primers also confirmed that B.

pertussis did not contain an aroD gene, supporting the concept that there was only one gene encoding a 3-dehydroquinase in B, pertussis.

The sequence discovered by Rosetti (1997) was confirmed in a BLAST search using the published complete genome sequence of Bordetella pertussis [ORF 5420 (http://pedant.gsf.de/cgi bin/wwwfly.pl?Set=Bordetella_pertussisTohama_I&Page=index) Last updated: 2002-01-07 14:25:19].

Roberts et al. (1990) reported the construction of an aroA mutant of B. pertussis which was found to be effective in eliminating small numbers of virulent challenge parent microorganisms (ranging from 103-104 CFU per mouse) from the lungs of mice vaccinated with 3 doses of the mutant strain by the intranasal route. This strain was apparently considered to be unsuitable as a vaccine candidate because of its complete disappearance from most immunised mice by essentially day 4 post-aerosolisation into the lungs.

The aroQ mutant generated in this investigation was found to survive in the reasonable numbers in all vaccinated mice at least until day 11 and in some mice until day 12 post-intranasal instillation. Challenge of mice immunised with three doses of the aroQ vaccine resulted in complete clearance of the virulent strain at day 6 post-challenge. The number of challenge microorganisms administered to mice in this investigation was nearly 500-fold higher than the aroA mutants lodged in the mouse lungs in experiments conducted by Roberts et al. (1990). Since the challenge dose administered to mice by WO 03/060105 PCT/AU02/01768 Roberts et al. (1990) was via aerosolisation of the B. pertussis culture, it is difficult to compare the present data with theirs. In fact Guiso et al. (1999) have reported that the intranasal mode of administrating the challenge microorganism, also employed in this investigation, is a better predictor of protection in human infants than the aerosolisation method used by Roberts et al. (1990). Investigators from the Bordetella Laboratory, Pasteur Institute and SmithKline Beecham Biologicals (Guiso et al. 1999) reported that the tricomponent acellular vaccine containing FHA, PT (Toxoid) and pertactin (PRN) were significantly superior at clearing bacteria from the lungs of infected mice than the bicomponent vaccines containing FHA and PRN. These authors also reported that the efficiency of bacterial clearance was dose-dependent. The bacterial clearance pattern was almost identical to that reported by Guiso et al. (1999) and followed a similar trend in mice immunised with the aroQ mutants in this investigation. Mice immunised with the aroQ B.

pertussis mutants also elicited antibodies against the two major virulence factors, FHA and PT, tested in this investigation. However since the aroQ mutant also possessed pertactin, an antibody response is also expected to be generated against this protein as well in immunised mice. The generation of CMI response in mice immunised with the aroQ B.

pertussis before or after challenge with virulent B. pertussis is clearly an advantage not reported previously with any other live attenuated mucosal vaccine against whooping cough.

An attenuated strain of B. pertussis, designated BPRA, with a chromosomal deletion in the pertussis toxin gene was reported recently to be successful in producing long-lasting protection in the mouse model (Mielcarek et al. 1998). However, the protection observed was not equivalent to that induced by the wild-type B. pertussis (Mielcarek et al. 1998). This result indicated that even though pertussis toxin was not essential for protection, it definitely played an important role in protection against whooping cough. The aroQ mutant generated and tested in this investigation was found to produce FHA, PT and PRN and as such constitutes an ideal vaccine strain for further development and evaluation as a potential vaccine against whooping cough. For example, in accordance with the information described herein, it is possible to construct a vaccine strain that not only incorporates two genetically unlinked mutations in order to prevent reversion of the mutant strain to a wild-type phenotype but also to detoxify the major toxins such as pertussis toxin and adenylate cyclase. It is also possible to raise therapeutic WO 03/060105 PCT/AU02/01768 -71 antibodies using standard methodologies for treating infections caused by Bordetella species, or related organisms.

In summary, the genetically modified Bordetella strain of the present invention, when administered by the noninvasive intranasal route, has the following properties: The strain enhances the level of IL-2, IL-12 and IFN-y post challenge (IFN- 7 y present pre-challenge) indicating a Thl response; IL- 12 induces the synthesis of IFN-y, induces growth of Tc and B cells and activates NK cells; IFN-y produced by Thl cells is a potent activator of NK cells and macrophages and is necessary for clearance of B. pertussis from the lungs; and high levels of the IgA type antibodies in the lung homogenates and significant production of interleukins 2 and IFN-%y, the use of the attenuated vaccine strain appears to be an ideal potential vaccine against whooping cough.

The disclosure of every patent, patent application, and publication cited herein is hereby incorporated herein by reference in its entirety.

The citation of any reference herein should not be construed as an admission that such reference is available as "Prior Art" to the instant application Throughout the specification the aim has been to describe the preferred embodiments of the invention without limiting the invention to any one embodiment or specific collection of features. Those of skill in the art will therefore appreciate that, in light of the instant disclosure, various modifications and changes can be made in the particular embodiments exemplified without departing from the scope of the present invention. All such modifications and changes are intended to be included within the scope of the appended claims.

WO 03/060105 PCT/AU02/01768 -72-

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Claims (24)

1. A genetically modified Bordetella strain having a partial or complete loss of 00 function in the endogenous aroQ gene and a lower capacity to propagate in a mammalian host but remaining viable in the host for a period of time sufficient to NO induce an immune response against a pathogenic Bordetella strain.

2. The genetically modified strain of claim 1, wherein the pathogenic Bordetella strain is a natural counterpart of the genetically modified Bordetella strain, or is a 0 10 related pathogenic Bordetella organism.

3. The genetically modified strain of claim 1, wherein the pathogenic strain is selected from Bordetella avium, Bordetella bronchiseptica, Bordetella holmesii, Bordetella parapertussis and Bordetella pertussis.

4. The genetically modified strain of claim 1, wherein the pathogenic Bordetella strain is Bordetella pertussis. The genetically modified strain of claim 1, comprising a disruption in the endogenous aroQ gene.

6. The genetically modified strain of claim 5, wherein the disruption has been introduced into the genome of a pathogenic strain of Bordetella by homologous recombination with a DNA targeting construct such that the targeting construct is stably integrated in the genome, wherein the disruption of the aroQ gene results in a reduced level and/or functional activity of the 3-dehydroquinase.

7. The genetically modified strain of claim 5, comprising an exogenous nucleic acid sequence in its genome, or on an extrachromosomal element, which is capable of abolishing or otherwise reducing the expression of aroQ or the level and/or functional activity of the 3-dehydroquinase encoded by aroQ. -76- 00

8. The genetically modified strain of claim 5, comprising an exogenous nucleic N acid sequence in its genome, or on an extrachromosomal element, which is capable of abolishing or otherwise reducing the expression of aroQ or the level and/or functional 00 activity of the 3-dehydroquinase encoded by aroQ, wherein the nucleic acid sequence comprises at lest a portion of aroQ, in the sense or anti-sense orientation, which is IND operably linked to a transcriptional control element.

9. The genetically modified strain of claim 1, further having a partial or complete C loss of function in at least one other endogenous gene selected from a pur gene, 0 10 another aro gene, a pertussis toxin gene, or any other gene which contributes to survival in the host and/or to bacterial virulence, or a combination thereof. The genetically modified Bordetella strain of claim 1, comprising at least one exogenous gene which is capable for expressing an antigen that is heterologous or foreign to the Bordetella strain.

11. The genetically modified Bordetella strain of claim 10, wherein the heterologous or foreign antigen is derived from a pathogen that is unrelated to the Bordetella strain.

12. The genetically modified Bordetella strain of claim 10, wherein the heterologous or foreign antigen is derived from a pathogen that infects by the mucosal route.

13. An isolated polynucleotide comprising a nucleotide sequence set forth in SEQ ID NO: 1 or 3, or is complementary to the sequence set forth in SEQ ID NO: 1 or 3.

14. An isolated polynucleotide comprising a nucleotide sequence having at least sequence identity to at least a portion of the sequence set forth in SEQ ID NO: 1 or 3, wherein said portion is at least 150 nucleotides in length. -77- 00 The isolated polynucleotide of claim 14 wherein the nucleotide sequence has at least 90% sequence identity at least a portion of the sequence set forth in SEQ ID SNO: 1 or 3, wherein said portion is at least 150 nucleotides in length. 00

16. A nucleic acid construct for disrupting an aroQ gene in a Bordetella cell, N comprising: a) a non-homologous replacement portion; b) a first homology region located upstream of the non-homologous replacement portion, the first homology region having a nucleotide sequence with substantial identity to a first aroQ gene sequence; and c) a second homology region located downstream of the non- homologous replacement portion, the second homology region having a nucleotide sequence with substantial identity to a second aroQ gene sequence, the second aroQ gene sequence having a location downstream of the first aroQ gene sequence in a naturally occurring endogenous aroQ gene of the Bordetella cell.

17. The construct of claim 16, wherein the aroQ gene comprises the isolated polynucleotide as set forth in any one of claims 13 to

18. A vector comprising a nucleotide sequence that corresponds or is complementary to the isolated polynucleotide sequence as set forth in any one of claims 13 to

19. The vector of claim 18, wherein the vector is a DNA targeting vector. A host cell containing the construct of claim 16 or claim 17 or the vector of claim 18 or claim 19.

21. A method for producing a genetically modified Bordetella strain, comprising introducing the nucleic acid construct of claim 25 into a Bordetella cell under conditions such that the nucleic acid construct is homologously recombined into the aroQ gene in the genome of that cell to produce a genetically modified Bordetella cell containing a disrupted aroQ gene. -78- 00

22. The method of claim 21, wherein the genetically modified Bordetella cell containing the homologously recombined nucleic acid construct is further characterised by expressing reduced or undetectable levels of aroQ. 00

23. The method of claim 21, wherein the genetically modified Bordetella cell N lacks the ability to produce a functional 3-dehydroquinase encoded by said aroQ gene.

24. A composition, comprising the genetically modified Bordetella strain of claim C 10 1, together with a pharmaceutically acceptable carrier. The composition of claim 24, further comprising an adjuvant.

26. A composition of matter comprising dendritic cells which have been exposed to the genetically modified Bordetella strain of claim 1 for a time and under conditions sufficient to express a processed or modified antigen derived from the Bordetella strain for presentation to, and modulation of, T cells.

27. The composition of matter of claim 26, which is in the form of an in vitro cell culture.

28. A method for modulating an immune response, comprising administering to a patient in need of such treatment an effective amount of the genetically modified Bordetella strain of claim 1, or the composition of any one of claims 22-25 or the composition of matter of claim 26.

29. A method for the treatment and/or prophylaxis of whooping cough or related condition, comprising administering to a patient in need of such treatment an effective amount of the genetically modified Bordetella strain of claim 1, or the composition of any one of claims 22-25 or the composition of matter of claim 26.