AU2002301207B2 - Antiviral protease inhibitors - Google Patents

Description

AUSTRALIA

PATENTS ACT 1990 DIVISIONAL APPLICATION NAME OF APPLICANTS: Medivir AB ADDRESS FOR SERVICE: DAVIES COLLISON CAVE Patent Attorneys 1 Little Collins Street Melbourne, 3000.

INVENTION TITLE: "Antiviral protease inhibitors" The following statement is a full description of this invention, including the best method of performing it known to us: -1- ANTIVIRAL PROTEASE INHIBITORS This is a divisional of Australian patent application No. 69356/98, the entire contents of which are incorporated herein by reference.

Technical field This invention relates to novel protease inhibitors and in particular to inhibitors of the aspartate protease possessed by certain retroviruses, notably HIV. The invention further relates to the use of such protease inhibitors in the treatment of conditions caused by retroviruses and in the preparation of medicaments for this purpose. The invention also relates to novel synthesis methodology for the facile preparation of protease inhibitors and similar chemical structures.

Background of the Invention Many biological processes are dependent upon the accurate enzymatic abscission of polypeptides at particular amino acid sequences. An example of such an operation is the post-translational processing of the gag and gag-pol gene products of the human immunodeficiency virus HIV to allow for the organisation of core structural proteins and release of viral enzymes. The enzyme responsible for this task, HIV protease, is a virally encoded homodimeric protease belonging to the aspartic protease family of enzymes. The human renin and pepsin enzymes also belong to this family. Inhibition of the HIV protease in cell culture prevents viral maturation and replication and thus this enzyme represents an attractive target for antiviral therapy against HIV in humans.

There are several references in the patent literature describing inhibitors of HIV protease., typically peptidomimetics having a large number of chiral centres. For example, Abbott Laboratories have extensively investigated linear peptidomimetics as described in a series of patent publication commencing with EP 402 654 and culminating in Abbott's application no. WO 94 14436 describing optimized linear peptidomimetics and in particular the compound Ritonavir: -1A- SH OH N Norvir as this compound is now known, is registered by the FDA and although it has good clinical efficacy, its synthesis is arduous. The synthesis difficulties which have characterized 2 prior art protease inhibitors can be understood by referring to Roche's protease inhibitor saquinavir (Inverase): According to literature reports, this compound requires a synthesis route of some 20 steps resulting in an overall yield reputed to be around 2 This difficult synthetic availability will put pressure on treatment cost and production capacity.

Merck's US patent no. 5 413 999 describes indanyl pentamine compounds, including its currently marketed product indinavir:

N

Merck's EP 480 714 discloses a symmetric protease inhibitor having terminal indanotamine groups spaced by a 7 carbon backbone: These compounds are prepared by complex methodology starting from an alkenediol.

Banyu's Japanese patent application no 7242613 A also describe symmetric protease inhibitors having indanolamine terminal groups spaced by a 7-carbon backbone: 3 o where R is H or lower alkyl. These compounds are prepared by BuLi-alkylation of N,Oisopropylidine-N-[2(R)-hydroxy-(lS)-indanyl]-3-phenylpropanamide with 2-chloro-2chloromethyl-propene, followed by ozonation, reduction and deprotection.

Vertex' international patent application no WO 94/13629 explores the use of a mannitol carbohydrate precursor to prepare compounds of the formula: H 0 Val 0 O 0 Val O H It will be apparent that with these inhibitors, the benzoyl moieties esterified to the C-1 and C-6 hydroxy groups of the mannitol precursor are intended to fill the PI and Pl' pockets of the HIV protease active site. Amino acid functions, such as valyl, are amide bonded to the C-2 and C-5 hydroxy groups and are intended to fill the P-2 and P-2' pockets of the enzyme.

These compounds are prepared by bridging the 3-and 4-hydroxy groups with isopropylidine, epoxidising and ring opening the terminal hydroxy groups with a nucleophile such as aryl alcohol followed by amidation of the resulting free hydroxy groups with a respective amino acid. Alternatively the isopropylidine-protected mannitol is first amidated on the more active C-l and C-6 (terminal) hydroxy groups with the amino acid P-2 filling groups and then esterified with the benzoyl moities on the C-2 and C-5 hydroxy groups.

One of Abbott Laboratories' early patent publications in the protease field, EP 402 646, describes a great number of potential approaches to the construction of protease inhibitors.

One of these approaches also employs a carbohydrate precursor which becomes the central backbone of a symmetric protease inhibitor. Examples 305 and 307 of EP 402 646 describe the ring-opening of a mannosaccharodilactone and the addition of terminal valine esters to 4 form a 3,4-O-isopropylidine-bridged adipamide derivative. The aryl groups which are to fit into the P1 and PI' pockets of the protease are added subsequently via triflate activation at the C-2 and C-5 positions of the carbohydrate backbone and these are later converted to phenylthio groups before the isopropylidine bridge is removed.

OH 1. Valine benzyl ester 0 2. H,S0 4 Dimethoxypropane 0 0 3. Triflic anhydride

HO

CF3

CF

0=S=0 O=S=O S0 0 0 0 0 S S 0 00 01r0 OHO OH O 1 The drawbacks with this process are the inevitable inversion of the configurations of C-3 and C-4 and that while reagents such as thiophenyl can be used to displace the triflate leaving group in the manner shown in EP 402 646, this can only produce thioether derivatives for the PI and PI' filling groups. At a superficial level it could be thought that the triflate leaving group could be displaced with conventional alkylating reagents such as alkoxide to give an O-alkylated P1/P1' filling group. However we have discovered in this prior art process that the use of alkoxide tends to eliminate the triflates producing an olefin, instead of the desired O-alkylated substituent.

Magnus Bjorsne et al in "Synthesis of Potential Candidates for Therapeutic Intervention against the Human Immunodeficiency Virus", Stockholm University, 1995 describes the compound Ph o OH o 0 o 0 OH 0 Ph the corresponding benzyl ester and the phenylalanine analog. These compounds are prepared from an L-mannaric acid precursor via the steps of i) bridging the C-3 and C-4 hydroxyls of the hexitol with isopropylidine, ii) protecting the C-i and C-6 primary hydroxyls, iii) O-alkylating the C-2 and C-5 hydroxyls to the aralkyl ethers, iv) oxidating the C-1 and C-6 primary hydroxyls to carboxylic acids; and v) condensing the resulting compound with the appropriate amino acid (ester) terminal groups. This methodology may be graphically represented as follows: HO HO OH O HO OH H 0 OH Hd "oH o o o o Ph Ph Ph o° 0 OH 0 0 O

HO

HOOH

O O HO- Ph -r O Ph The appropriate valine or phenylalanine (ester) end unit is then condensed onto the terminal carboxyls in dichloromethane-THF using HOBt-EDC coupling conditions. Despite the need for protection, oxidation and deprotection steps, the Bjirsne synthesis methodology is an improvement over the very large number of steps in conventional peptidomimetic synthesis (see the discussion of saquinavir above). The Bjbrsne process also avoids the triflate activation, the preferential reactivity of the "wrong" C-3 and C-4 atoms and other drawbacks of the Abbott EP 402 646 process. However the compounds proposed by Bjdrsne have inadequate antiviral properties. The best Bjdrsne compound, where the terminal amines are valine methyl esters (depicted above), has an ICso of 5 iM which should be compared to currently marketed protease inhibitors which have IC 5 o values one or more orders of magnitude lower.

We have now discovered a novel group of compounds with antiviral properties in the nanomolar ICso range and which lend themselves to a novel carbohydrate based synthesis technique which is even more convenient than those of the prior art discussed above.

6 Accordingly, a first aspect of the invention provides novel compounds of the formula I: A A" ©i Y wherein: A' and A" are independently a group of the formula II:

R"'

R

II

wherein: R' is H, CH3, C(CH 3 2 2 or -DP is H, CH 3 R' is H, C 1

-C

3 alkyl; D is a bond, C 1 3 alkylene, or P is an optionally substituted, mono or bicyclic carbo- or heterocycle; R" is H, any of the sidechains found in the natural amino acids, carboxacetamide, or a group

(CH

2 )nDP; M is a bond or Q is absent, a bond, -CH(OH)- or -CH2-; or R" together with Q, M and R' define an optionally substituted 5 or 6 membered carbo- or heterocyclic ring which is optionally fused with a further 5 or 6 membered carbo- or heterocyclic ring; with the proviso that R' is -N(CH 3 2 -N(Ra)OR' or -DP if M is a bond and Q is absent; X is H, OH, OCH 3 Y is H, OH, OCH3, but X and Y are not both H; Z' and Z" are independently -(CH 2 )mP where P is as defined above; n and m are independently 0,1 or 2; and pharmaceutically acceptable salts and prodrugs thereof.

7 Compounds of the formula I are active inhibitors of aspartyl proteases, such as those from HIV. Further aspects of the invention thus provide: a pharmaceutical formulation comprising a compound of the formula I in admixture with a pharmaceutically acceptable carrier or diluent; the use of a compound of the formula I in the manufacture of a medicament for the prophylaxis or treatment of conditions, such as AIDS caused by retroviruses, such as HIV; and a method for treating conditions caused by retroviruses, especially AIDS in humans, comprising administering a compound of formula I to a subject afflicted with said condition.

The compounds have a relatively low molecular weight and should therefore provide good oral absorption properties in mammals. In contrast to prior art aspartyl protease inhibitors, the compounds of the invention can be conveniently prepared with a small number of steps from readily available and cheap starting materials, such as L-mannarodilactone or its commercially available precursors or derivatives, such as L-mannonic-y-lactone.

A further aspect of the invention thus provides a method for the preparation of a compound of the formula I -A A"

V

where X, Y, Z' and Z" are as defined above and each of A' and A" are independently: a group of the formula II or a conventional protease P-2/P-2' filling group, the method comprising i) O-alkylation of an L-mannaric-1, 4 :6,3-di-lactone to form the Z' and Z" groups, 8 ii) opening of the lactone with similar or different primary or secondary amines to form the respective A' and A" groups; and iii) optional conversion of the C-3 and C-4 to the appropriate X and Y" groups.

An example of this method aspect of the invention can be depicted as follows: HO H N HO O O O 0.Y 0 8" 0 HO OH HO

H

0 0o O OH 0 OH 0 r Ph Ph The method aspect of the invention is preferably used to produce the compounds of formula I as claimed herein, but can also be used to prepare protease inhibitors with conventional protease P-2/P-2'filling groups.

Introduction of the Z' and Z" groups as ethers of the C-2 and C-5 hydroxyls in step i) is conveniently carried out by O-alkylation with the appropriate derivative:

E(CH

2 )nP where E is a halogen, mesylate, tosylate etc and P and n are as described above in the presence of a base such as a carbonate, metal hydride or hydroxide and an aprotic solvent such as N,Ndimethylformamide, tetrahydrofuran or acetone. Conveniently the alkylating agent is benzyl trichloroacetiridate in conjunction with a proton or a Lewis acid, e.i. trimethylsilytriflate.

The ring opening in step ii) to introduce the amino or amino acid derivatives A' and A" are carried out using standard conditions in solvents such as dioxane, nitromethane

THF,

9 diglym, DMF or DMSO, which are preferably chosen to dissolve both the carbohydrate derivative and the particular amine involved.

For ease of synthesis it is generally preferred that the terminal amines A' and A" are identical. However, although the target enzyme is a symmetric dimer, thus implying a tight interaction with symmetric compounds, it can in some circumstances be advantageous for resistance or pharmacokinetic reasons etc to have asymmetric terminal amines. Where is it is desired to have an asymmetric compound, that is where the A' and A" groups differ, it will generally be most convenient to add the respective A' and A" groups sequentially. This can be done in conjunction with appropriate protection of one of the rings of the dilactone, but may also be achieved by manipulation of the reagent concentrations, reaction conditions, speed of addition etc to provide a monoaminated lactone which is separated by conventional techniques or amine neutralization, prior to reaction with the second A' or A" amine.

Alternatively the differential terminal amination can be achieved with a solid phase synthesis where the unaminated or partially aminated lactone is secured to a solid phase substrate, such as polymer beads of which many are known in solid phase chemsitry, for instance Merrifield resin. Immobilization of the lactone in this fashion will only allow amination on a defined lactone ring. Scheme I below outlines such a scheme in the context of combinatorial synthesis of a library of compounds in accordance with the invention, but it will be apparent that single pure compounds can be prepared by similar methodology, but using pure reagents.

SCHEME 1 1 0 0 EDC/NHS 0 OH 01 01 0

H

2

N(CH

2 )nN H 2 2

H

2 N i

N

xNH 2 0

~H

2 N a N ~'NH-RNH 2

NH

3 1. N-Boc-amino acids 2. deprotection 00 00 0x" 0 0 4 0 0 0 I NH-RNH-XHN 0 0 1,1 Ry

H

2 NRa, H 2 NRb, H 2 NRc, etc Rx 0 OHO0 H OH -tNH-RN ~vI-FHNN 0 0 0 OHO0 I I In Scheme 1, steps 1 and 2 comprise the preparation of a linking group on the carboxy groups of the resin beads. Linkers of various lengths, rigidities and differential cleavabilities (several are listed against step 2) can be used as is conventional in the solid phase chemistry art. In step 3, the free amine on the linker-equipped solid phase bead is amide bonded with an N-protected amino acid under standard peptide chemistry conditions. The amino acid reagent comprises a number of different, protected amino acids, such as N-Boc valine, N- Boc isoleucine, N-Boc alanine, N-Boc leucine etc and thus this reaction step results in a first combinatorial chemistry tier. The solid phase beads now bear a plurality of randomly disposed amino acids each spaced by a respective linker from the carboxy surface of the bead.

In step 4 the free amine (after deprotection of the N-Boc groups) on the amino acid array is used to ring-open a di-O-alkylated y-dilactone, wherein R, and Ry are the same or different optionally substituted (hetero)arylalkyl groups, for instance benzyl, fluorobenzyl, pyridylmethyl etc. This ring opening is carried out under conventional conditions, as exemplified below, in solvents such as dioxane, nitromethane, THF, diglym, DM DMSO and the like. If R, and Ry are different (hetero)arylalkyl groups, this step will thus produce a further tier of combinatorial variation, depending on whether the or Ry -bearing ring is opened. However, the dilactone reagent itself may comprise a plurality of different dilactones with various combinations of Rx, Ry and/or stereochemistry of the lactone, leading to an even greater spread of combinatorial diversity.

In the fifth step, the remaining ring of the now-immobilized lactone is ring opened in a corresponding fashion with a further amine reagent. Once again this amine reagent may comprise a mixture of different amines, for instance L-amino acids or (hetero)cyclic amines such as the one depicted. Thus this step too may create a further tier of combinatorial diversity. The combinatorial library is cleaved from the linker using its appropriate cleavage reagent, typically a specific amidase or change in pH etc.

Although the compounds of formula I are preferably prepared by the method aspect of the invention, it is also possible to employ the initial steps of the Bjdrsne technique described above in conjunction with the appropriate choice of A' and A" amines, followed where necessary by postmodification of X and Y as discussed above and exemplified in the following Example 2.

12 Preparation of compounds of Formula I in which X is hydrogen can be conveniently done by deoxygenation as illustrated in the accompanying Examples 2 and 26. The preferred stereochemistry is the 2R, 3R, 4R, 5R form.

Carbocyclic groups for R' as -DP and/or and/or the optional substituents thereto may be saturated, unsaturated or aromatic and include monocyclic rings such as phenyl, cyclohexenyl, cyclopentenyl, cyclohexanyl, cyclopentanyl, or bicyclic rings such as indanyl, napthyl and the like.

Heterocyclic groups for R' as -DP and/or and/or the optional substituents thereto may be saturated, unsaturated or aromatic and have 1 to 4 hetero atoms including monocyclic rings such as furyl, thienyl, pyranyl, pyrrolyl, pyrrolinyl, pyrrolidinyl, pyrazolyl, pyrazolinyl, pyrazolidinyl, imidazolyl, imidazolinyl, imidazolidinyl, pyridyl, piperidinyl, pyrazinyl, piperazinyl, pyrimidinyl, pyridazinyl, oxazolyl, oxazolidinyl, isoxazolyl, isoxazolidinyl, morpholinyl, thiazolyl, thiazolidinyl, isothiazolyl, isothiazolidinyl, and the like or bicyclic rings especially of the above fused to a phenyl ring such as indolyl, quinolinyl, isoquinolinyl, benzimidazolyl, benzothiazolyl, benzoxazolyl, benzothienyl etc.

The carbo or heterocyclic ring may be bonded via a carbon or via a hetero atom, typically a nitrogen atom, such as N-piperidyl, N-morpholinyl etc.

Preferred embodiments of Formula II for the A/A" groups of the compounds of the invention include those of the formula Ha or He: o rr IIa where n is 1 or 2 and R' is alkyloxy, preferably methyloxy, or those where n is 0 and R' is methyl.

Other preferred groups of formula II include [lb below

OH

N N

-CH,

R" 0 lib An alternative preferred configuration for the A'A" groups of the compounds of the invention includes groups of the formula TIc: R

I

/N

/Q

S R' tic

R"

where Q is a bond, methylene or R' is 2 -NR' OR', where R' is H or

C,-C

3 alkyl, or a carbo- or heterocyclic group including N-piperidine, N-morpholine, Npiperazine, pyrrolyl, imidazolyl, pyrazolyl, pyridyl, pyrimidyl, pyrazinyl etc, and is hydrogen or methyl.

A favoured subset of compounds within formula IIc has the formula lid:

H

N RB R Rd lid where Rd is hydrogen or methyl (that is a valyl or isoleucyl side chain) and R' is -N x N or

N

-N

where X is methylene, 0, S, S=O, S(=0)2 or NH or R' is -N(CH 3 2 -NHOH, -NHOMe, NHOEt, -NMeOH, -NMeOMe etc.

In each of formulae IIa, IIb and IIc, R" is hydrogen, methyl, ethyl, isopropyl, cycloalkyl such as cyclopropyl, cyclobutyl or cyclohexyl, cycloalkenyl, benzyl, carboxacetamide or 4imidazolylmethy, any of which may be substituted as defined above. Preferred R" groups include the side chains found in the natural amino acids, especially those of leucine, asparagine, histidine or proline. The most preferred R" groups for formula Ia, IIb, Ic and IId are the isoleucyl and especially the valyl side chain.

R' will vary depending on the nature of Q and/or M, if present, and may for instance be selected from hydrogen, methyl, ethyl, isopropyl, R' as defined above, valinol, a heterocycle such as pyridyl, thiazole, oxazole, imidazole, N-piperidine, N-morpholine, N-piperazine, pyrrolyl, imidazolyl, pyrazolyl, pyrimidyl, pyrazinyl, any of which R' groups may be substituted as defined for below.

Further favoured groups include those of formula II where Q, M and R' together define an optionally substituted 5 or 6 membered carbo- or heterocylic ring. A preferred group within this definition include groups within formula III: R2 R1 Ni,. R6 R

III

where is as defined above, R' is H, NR 4

R

4

C(=O)R

3

CRR

4 or a monocyclic, optionally substituted carbo- or heterocycle;

R

2 is OH, or together with R' is or if R' is NR 4

R

4 then R 2 may be H;

R

3 is H, halo, CI-C 3 alkyl, OR 5

NR

4

R

4

R

4 is H, Ci-C 3 alkyl;

R

5 is H or a pharmaceutically acceptable ester;

R

6 is OH, NH 2 carbamoyl or carboxy;

R

7 is hydrogen, CI-C 4 straight or branched alkyl or together with the adjacent carbon atoms forms a fused phenyl or heteroaromatic ring; Preferred groups of formula III include aminoindanol and l-amino-azaindan-2-ol, that is moieties of the formulae: OH

OH

-7 or Conventional protease P-2/P-2' filling groups for include those found in Roche's saquinavir and Abbott's ritonavir compounds. Additional examples of conventional P-2/P-2' filling groups include those found in Vertex' VX 478, Agouron's AG1343 (now known as nelfinavir) and Merck's indinavir, as depicted above.

Optional substitutents for the carbo- or heterocyclic moiety of or include one to three substituents such as halo, amino, mercapto, oxo, nitro, NHCi-C 6 alkyl, N(CI-C 6 alkyl), Cl-C 6 alkyl, CI-C 6 alkenyl, CI-C 6 alkynyl, CI-Cs alkanoyl, CI-C 6 alkoxy, thioC,-C 6 alkyl, thioCI-C 6 alkoxy, hydroxy, hydroxyC 1 -C alkyl, haloC-C 6 alkyl, aminoC-C 6 alkyl, C,-C 6 alkyl, cyano, carboxyl, carbalkoxy, carboxamide, carbamoyl, sulfonylamide, benzyloxy, morpholyl-Cl-C 6 alkyloxy, a monocyclic carbo- or heterocycle, as defined above, a carbo- or heterocyclic group spaced by alkyl, such as C 1 .3 alkylaryl, etc.

The preferred definitions for Z' and Z" include benzyl, unsubstituted or substituted with 1, 2 or 3 substituents, especially 1 selected from fluoro, chloro, hydroxy, amino, -NH(CI.6 alkyl),

-N(CI.

6 alkyl) 2 -NPh(C 1 6 alkyl), -NHPh, methoxy, cyano, hydroxymethyl, aminomethyl, alkylsulfonyl, carbamoyl, morpholinethoxy, benzyloxy, benzylamide etc. Other possibilities exhibiting the great freedom in this area are shown in the examples. It will be apparent that the substituent to Z' and/or Z" may comprise a ring structure (which substituent ring structure is itself substituted as defined herein) such as phenyl or a 5 or 6 membered heterocycle containing one or two hetero atoms such as thiophene, pyridine etc. The preparation of useful heterocyclic substituents for Z' and Z" as benzyl are described in Tetrahedron Letters 1997 6359-6359-6367 and J Org Chem 62 (1997) 1264 and 6066, including N-morpholine, N-piperidine, N-piperazine, N'-methyl-N-piperazine,

N-

pyrrolidone, N-pyrrolidine and the like.

Such substituents may be in the meta but especially the ortho or para positions of with small groups such as fluoro being favoured for the ortho and meta and with extensive freedom for larger groups in the para such as (optionally substituted) cyclic substituents, 16 including the N-bonded rings in the immediately preceding paragraph. The whole Z' and Z" group or their respective carbo-or heterocyclic moiety may be different but for ease of synthesis it is convenient if they are the same.

Appropriate pharmaceutically acceptable salts, both for as a free acid or for other charged groups along the compound of formula I include salts of organic carboxylic acids such as acetic, lactic, gluconic, citric, tartaric, maleic, malic, pantothenic, isethionic, oxalic, lactobionic, and succinic acids, organic sulfonic acids such as methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, p-chlorobenzenesulfonic acid and ptoluenesulfonic acid; and inorganic acids such as hydrochloric, hydroiodic, sulfuric, phosphoric and sulfamic acids.

Prodrugs of the invention are derivatives that release a compound of formula I in vivo, generally by hydrolysis or other metabolic interaction in the intestine, liver or plasma.

Typical prodrugs are esters formed on free hydroxy groups in the compounds.

Appropriate pharmaceutically acceptable esters include CI-C22 fatty acid esters, where the fatty acid is unsaturated, monounsaturated or multiply unsaturated. Saturated fatty acid esters include short chains such as acetyl or butyryl or long chain such as stearoyl.

Unsaturated fatty acid esters are preferably in the to-9 series, such as palmitoleic or linolenic esters. Other esters include CI-Cs alkylaryl esters such as benzyl or methylpyridyl or esters of phosphoric acid, such as monophosphate. Alternative esters include the corresponding fatty acid or alkylaryl carbonate, carbarnate or sulphonic esters.

Presently favoured compounds of Formula I include N1,N6-dif[(S)-2-methyl- -(methylcarbamoyl)propyl]-(2R,3R,4R,5R)-2,5-di(benzyloxy)-3,4dihydroxyhexanediamide, N1,N6-di [(1S)-2-methyl- -(methylcarbamoyl)propyl]-(2R,3S,5R)-2,5-di(benzyloxy)-3hydroxyhexanediamide, N 1,N6-di[(S)-2-methyl--(methylcarbamoyl)propy]]-(2R,3R,5R)-2,5-di(benzyloxy)-3hydroxyhexanediamide, N1,N6-di[( S)-2-methyl- 1-(methylcarbamoyl)butyl]-(2R,3R,4R,5R)-2,5-di(benzyloxy)-3,4dihydroxyhexanediamide, NI,N6-di[( 1S)- l-(methylcarbamoyl)-2-phenylethyl-(2R,3R,4R,5R)-2,5-di(benzyloxy)-3,4dihydroxyhexanediamide, 17 NI ,N6-di I-(methYlcarbamoyl)-2(4-hydroxyphenyI)ethyl]-(2R, 3R,4R,SR)-2,sd i(benzyloxy)-3,4-dihycdroxy hexanedi amide, NI ,N6-di S)-2-merhyi- i-(methYlcarbarnoyl)propyllp(2R,3R,4R,5R)-25di fluorobenzyl)oxy]-3,4-dihydroxyhexanediamide, NI ,N6-di[(lIS)-2-m 'ethyl- Il-(cYcloPropycarbaroy)propy(R34,5R)- 2 5 di(berizyloxy)-3,4-di hydroxyhexanediarnide, Ni ,N6-di[( 1 S)-2-methyl- I -(methylcarbamoy)propyiy-(2p3R,4R 5R)-2,5-di methylbenzyl)oxyj-3 4 -dihydroxyhexanediamide, NI ,N6-di S 2 R)-2-hydroxy-2,3-dihydro. 11-1 -indenYI-(2R,3R,4R5R)-2,5-di(benzyloxy)- 3 4 -dihydroxyhexanediamide, N1- [(IS ,2R)-2-hydroxy-2,3-dihydro- I -indenyl]-N64[( 1S)-2-methyi-

I-

(methylcarbarnoyl)propylp-(2R,3,R5,-,-ibez x)34dhdrxhxndaie Ni ,N6-d 1 S,2R)-2-hydroxy-2,3-dihydro- I H- I -indenY]-(2R,3R,4R,5R)-2,5d( 2 flu orobenzyloxy)-3 ,4-d ihydroxyhexanediamide, NI ,N6-di[( 1 S,2R)-2-hydroxy-2,3-dihydro. IN- I -indenYI-(2R,3R,4R5R)2,5di(4 flu orobenzyloxy)-3 4 -dihydroxyhexanediamide, NI ,N6-di S,2R)-2-hydroxy-2,3-dihydro- 111-1-indenyl]-(2R,3R,4R 5R)-2- (4-methy Ibenzyloxy)-3 4 -dihydroxyhexanediamide, Ni ,N6-di S)-2-rnethyl- 1 -(methylcarbamoyl)propyip-(2R3R,4R 5R)-2,5-d i[(4phenylbenzyl)oxy]-3,4-dihydroxyhexanediamide, Ni ,N6-di 1(1 S)-2-rnerhyl- I -(methylcarbamoy)propyj(2R3g4R, 5 R)-2,5-di th ienyi)ben zyloxyj-3 .4-dihydroxyhexanediamide, Ni ,N6-di 1 -phenyl- I -(methYlcarbaoyl)mehYl2R3R4RR)2 5 di(benzylx)- 3,4-d ihydroxyhexanediamide NI ,N6-di[(iS )-2-niethyl. I-(methyicarbamoy I)propyI)-(2R,3R,4R,5R)-2,5-di fluoroben zyl)oxy]-3 4 -dihydroxyhexanediamide NI ,N6-di iS, 2 R)-2-hydroxy-2,3-dihydro 1 H-I -indenyI-(2R.3R,4R5Ry-2,5d(3.

fluorobenzyioxy)-3,4aihydroxyhexanediarnide NI ,N6-di S)-2-znethyl- I -(me-thyicarbamoyl)propyjp-(2R 3R,4R SR)-2,5-di fluoroben zy l)oxyI- 3 ,4-dihydroxyhexanediamide NI ,N6-di[( I S)-2-methyl- I -methylcarbamoyl)propyI(2R,3R4RR- 2 5di[ 4 2 4 difluorobenzyl)oxy-3 4 -dihydroxyhexanediamide Ni ,N6-di[( I S ,2R)-2-hydroxy-2,3-dihydro.1H-I -indenY]-(2R,3R,4R,5R25di(2, 4 difluorobenzy lox y)- 3 ,4-dihydroxyhexan ediami de 18 NI ,N6-di S)-2-methyl- 1 -(methylcarbanoy1)propyIJ-(2R,3R,4R,5R.)-2 5-d i[4-(2pyridyl)benzyloxy]-3,4-dihydroxyhexanediamide NI ,N6-dir( I S)-2-methyl- 1 -(methylcarbamoyl)propyql-(2R,3R 4RSR)-2,5-di pyridyl)benzyloxy]-3 ,4-dihydroxyhexanediamide NIN6-di 1(1S)-2-metbyl-lI-(methylcarbamoyl)propyly-(2R,3R,4R,5R)-2,5-di nitrophenyl)benzy)oxy1-3,4-dihydroxyhexanedarnid 0 NI ,N6-di S)-2-methyl- I -(methylcarbamoyl)propyll}(2R,3R,4R,5R)-2,5-di thienyl)benzyloxy]-3,4-dihydroxyhexanediamide NI ,N6-di[(i S,2R)-2-hydroxy-2,3-dihydro- HU-1 -indenylI-(2R,3R,5R)-2,s.di (benzyloxy)-3hydroxyhexanediarnide Ni S,2R)-2-hydroxy-2, 3-dihydro- I H- 1indnl 6(2clr--lurbny and their pharmaceutically acceptable salts and prodrugs.

0-alkylated dilactone intermediates are also novel compounds and thus a further aspect of the invention provides compounds of the formula TV: z 0 0 0 0 0 z

IV

where Z' and Z" are as defined above. Preferably the compound of formula IV has the following stereochemnistry: Favoured compounds of formula IV include those where Z' and Z" are benzyl, 2fluorobenzyl, 2-methy lbenzyl 1 2 5 4-diflucrobenzyl, 4-fluorobeozyl, 4 -bromobenzyl, 4phenylbeuzyl, 4-thiophenylbeuzyl, -nitrophenyl)benzyl, 4 -(pyridyl)benzyl or benzyl parasubstituted with a primary, secondary or tertiary amine or an N-bonded heterocycle such as piperidine, morpholine etc. Alternatives to benzyl for Z' or Z" groups may comprise other arylC,- 2 alkyI or heteroaryl[C 3 2 alkyl such pyridyirneihylene, quinolyirnethylene or napthylmetylene as known in the P1 protease art.

As with formula 1, the Z' and Z" groups on the compounds of Formula IV may differ but it is convenient, and consistent with the bimeric nature of the target enzyme if they are the same.

Preferred intermediate compounds of Formula [V thus include: 2 7 5-di-O-benzyl-L-mannarol 1, 4 :6,3-dilactonec, fluoro benzy1)-L-mannaro 1 ,4:6,3 -d ilac tone, 2 5 -di-O-(2,4-difluorobenzyl>L-mannaro-

I,

4 :6.3-dilactone, 2 1 5 -di-O-(4-fluorobenzyl)-L-mannaro. I 4 :6,3-di-Iactone, 2 5 -di-O-( 2 -chlorobenzyl)-L-mannaro- 1,4: 6,3-dilactone, i-O-(4-chlorobenzyl)-L-mannaro- I,4:6,3-di-lacrone, 2,5-di-Q-(4-thiophen-3 '-yl-benzyJ)-L-mannaro- I,4:6,3-di-lactone (4-thiophen-2 '-yl-benzyl)-L-mannaro.

I,

4 6 ,3-di-Iactone, 2,5-di-O-(4-(thiazol-4' -y l)-benzyl)-L-mannaro- 1,4 6 ,3 -di-lac tone 2,5-di-O-(4-thiazol-2' -yI-benzyl)-L-mannaro-1,4: 6,3-di-lacrone,, 2 ,S-di-O-(4-phenylbenzy l)-L-mnannaro- 1,4: 6,3-di- lactone, 2,5-di-O-(4-pheny lbenzyl)-L-mannaro- 1,4: 6,3-di-lactone, 2 5 -di-O-(4-(4'-nitrophenylbenzyl).L-mannaro.

I,

4 :6,3-di-laccone, i-O0-(4- (4 '-cyanop hen ylben zyl).L- mannaro. I ,4 6 3 -di-Iactone, -halophenylbenzyl)-L-mannaro- 1 4 :6,3-di-Iactone, 2 5 -di-O-(4-(4'-aminophenylbenzys)L-mannaro) 1 ,4:6,3-di-Iactone, 2 ,5 -di-O0-( 4 '-carbox ypheny Tben zy>-L-man n 1 aro 0 1,4:6,3 -d i-1acrtone, -phenylbenzyl)-L-mannaro- 1 ,4 :6,3-di-lactone, 2.5-di-O-(4-pyrid-2-ylbenzyl)-L-mannaro- 1,4 :6,3-di-lacrone, (4-pyrid -3-y lben zy mannaro- 1,4 :6,3-di-lacrone, 2,5-di-O-(4-N-morpholinylbenzyl)-L-mannaro- 1,4: 6,3-di-lactone, 2,5-di-O-(4-N-pipeuidinylbenzyl)-L-mannaro- I ,4:6,3-di-Iactone, 2,5-di-Q-(4-N-piperazinylbenzyl)-L-mannaro- 1,4:6,3-di-lacrone, 2,5-di-O-(4-benzylbenzyl)-L-mannaro- 1 ,4:6,3-di-lacrone, and the l ike.

Intermediate compounds of formula III can be prepared by the following reaction scheme:

OH

H

2

N

YHO C

IN,

089 k N-prolection -N i Y 0 Oxidation

-CN

Hydrolyis 0 HO

R

R=NH2 N R=NHA

R=OH

N'-N

NH

Bu 3 SnN 3 HO N Y 0 xS Allanositnny HO x 1.03 2. reduction HO

F

0 H O

COOR

H2N.,,, ox.

m4

CN

or

COOR

COOR

CN

S

Aminohydroxylation HO CN H1 2 N

Y

x Y

OR

CISi(CH,),

N

N,,

E

l-amino-azaindan-2-ol P-2 filling groups can be prepared analogously to J Med Chem 1991 1228-1230:

N

CrO 3

H

2 SO4/AcOH POC13

N

J

,Il OH In treating conditions caused by retroviruses, the compounds of formula I are preferably administered in an amount to achieve a plasma level of around 10 to 1000 nM and more preferably 100 to 500 nM. This corresponds to a dosage rate, depending on the bioavailability of the formulation of the order 0.001 to 100 mg/kg/day, preferably 10 to mg/kg/day.

In keeping with the usual practice with HIV inhibitors it is advantageous to co-administer one to three additional antivirals, such as AZT, ddl, ddC, D4T, ritonavir, saquinavir, indinavir, nelfinavir, DMP 266, delavirdine, nevirapine, trovirdine, PFA, H2G etc. The molar ratio for such co-administered antivirals will generally be chosen to reflect the respective EC 5 0 performances of the antiviral. Molar ratios of 25:1 to 1:25, relative to the compound of formula I will often be convenient.

While it is possible for the active agent to be administered alone, it is preferable to present it as part of a pharmaceutical formulation. Such a formulation will comprise the above defined active agent together with one or more acceptable carriers and optionally other therapeutic ingredients. The carrier(s) must be acceptable in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient.

The formulations include those suitable for dral, rectal, nasal, topical (including buccal and sublingual), vaginal or parenteral (including subcutaneous, intramuscular, intravenous and intradermal) administration. The formulations may conveniently be presented in unit dosage form, e.g. tablets and sustained release capsules, and may be prepared by any methods well known in the art of pharmacy.

Such methods include the step of bringing into association the above defined active agent with the carrier. In general, the formulations are prepared by uniformly and intimately bringing into association the active agent with liquid carriers or finely divided solid carriers or both, and then if necessary shaping the product.

Formulations for oral administration in the present invention may be presented as discrete units such as capsules, cachets or tablets each containing a predetermined amount of the active agent; as a powder or granules; as a solution or a suspension of the active agent in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water in oil liquid emulsion and as a bolus etc.

With regard to compositions for oral administration tablets and capsules), the term suitable carrier includes vehicles such as common excipients e.g. binding agents, for example syrup, acacia, gelatin, sorbitol, tragacanth, polyvinylpyrrolidone (Povidone), methylcellulose, ethylcellulose, sodium carboxymethylcellulose, 23 hydroxypropylmethylcellulose, sucrose and starch; fillers and carriers, for example corn starch, gelatin, lactose, sucrose, microcrystalline cellulose, kaolin, mannitol, dicalcium phosphate, sodium chloride and alginic acid; and lubricants such as magnesium stearate and other metallic stearates, stearic acid, silicone fluid, talc waxes, oils and colloidal silica.

Flavouring agents such as peppermint, oil of wintergreen, cherry flavouring or the like can also be used. It may be desirable to add a colouring agent to make the dosage form readily identifiable. Tablets may also be coated by methods well known in the art.

A tablet may be made by compression or moulding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active agent in a free flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, preservative, surface-active or dispersing agent. Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. The tablets may be optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active agent.

Formulations suitable for topical administration include lozenges comprising the active agent in a flavoured base, usually sucrose and acacia or tragacanth; pastilles comprising the active agent in an inert base such as gelatin and glycerin, or sucrose and acacia; and mouthwashes comprising the active agent in a suitable liquid carrier.

Formulations suitable for topical administration to the skin may be presented as ointments, creams, gels, and pastes comprising the active agent and a pharmaceutically active carrier.

An exemplary topical delivery system is a transdermal patch containing the active agent.

Formulations for rectal or vaginal administration may be presented as a suppository or pessary with a suitable base comprising, for example, cocoa butter or a salicylate. Other vaginal preparations can be presented as tampons, creams, gels, pastes, foams or spray formulations containing, in addition to the active agent, such carriers as are known in the art to be appropriate.

Formulations suitable for nasal administration wherein the carrier is a solid include a coarse powder having a particle size, for example, in the range 20 to 500 microns which is administered in the manner in which snuff is taken, i.e. by rapid inhalation from a container of the powder held up close to the nose. Suitable formulations wherein the carrier is a liquid 24 for administration, for example, as a nasal spray or as nasal drops, include aqueous or oily solutions of the active agent.

Formulations suitable for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain antioxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example water for injection, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules, and tablets of the kind previously described.

Detailed Description of Embodiments of the Invention.

The invention will now be further illustrated by reference to the following non-limiting Examples.

EXAMPLE 1 NLN6-dif( lS)-2-methvl- -(methvlcarbamovl)propyl]-(2R.3R,4R,5R)-2,5-di(benzvloxy)-3,4dihydroxyhexanediamide.

HO H OH HN HO 0 -a c ±0 CI N 98 OC H N M S0 OH 0 0 CH3 OH O T 0 el OHO

C-

In short, oxidation of L-mannonic-y-lactone with nitric acid gave crystalline L-mannaric- 1,4:6,3-di-lactone which was dibenzylated with dibenzyl trichloroacetimidate and trimethylsilyltriflate to give 2,5-di-O-benzyl-L-mannaric-l,4:6,3-di-lactone. This lactone may be opened by the addition of any primary or most secondary amines, in this case by Nmethyl-L-valine to give the titled compound in 10-30% overall yield.

In more detail, the above synthesis scheme proceeded as follows: A. L-mannonic-y-lactone (8.1 g) in aqueous HNO 3 (120 ml) was kept at 98°C under a stream of nitrogen for 1-2 hours. After concentration to a sticky mass, water (50 ml) was added and the solution was concentrated to near dryness. This material was dried in a vacuum for 16 hours whereupon ethanol (5 ml) and ether (100 ml) were added and the solid was finely dispersed by sonification and mechanical grinding. The solution was taken off and the solid was washed with ethanol (10 ml). The remaining solid was recrystallised in ethanol to give 4.3 g of L-mannaric-1,4:6,3-di-lactone.

3 C-NMR (DMSO-d6): 6 69.2 (C2, C5), 75.9 (C3, C4), 174.3 (Cl, C6).

B. Trimethylsilyltriflate or trifluoromethylmethanesulphonic acid (44 drops from a Pasteur pipette) was added to a well stirred solution of L-mannaric-l,4:6,3-di-lactone (696 mg) and benzyl trichloroacetimidate (3040 mg) in dioxane (140 ml). After 1.5 hours the solution was filtered trhough a pad of I cm silica, I cm NaHCO3 and 1 cm silica, washed through with dioxane (20 ml), concentrated to dryness and recrystallised from CHC1I to give 1.85 of solid. This solid was slurried in hot ether and filtered. The remaining solid (900 mg) was collected as 2,5-di-O-benzyl-L-mannaric- 1,4:6,3-di-lactone.

"C-NMR (DMSO-d6): 5 72.3, 74.6, 75.1 (CH 2 0, C2, C3, C4, C5), 128.2, 128.3, 128.7, 137.2 172 (C1, C6).

C. A solution of 2,5-di-O-benzyl-L-mannaric-1,4:6,3-di-lactone (50 mg) and N-methyl- L-valine (110 mg) in acetonitrile (0.5 mi) was kept at 70 0 C for 16 hours. After cooling the product was purified by silica gel column chromatography (CHCIn-MeOH 9:1) to (35 mg).

EXAMPLE 2 Nl.N6-di IS)-2-methvl-I -(methvlcarbamovl)propvll-(2R.3S.5R)-2,5-di(benzvloxv)-3hydroxvhexanediamide.

A Preparation of (2R,3S,4S,5R)-2,5-di(benzyloxy)-3,4-O-isoproylidene-3,4dihydroxyhexanediacid.

To a solution of 2,5-di-O-benzyl-3,4-O-isopropylidene-L-iditol (1.84 g, 4.57 mmol) in

CHCI

2 (90 ml) was added TEMPO (36 mg, 0.23 mmol), potassium bromide (104 mg, 0.87 mmol), tetrabutylammonium bromide (163 mg, 0.51 mmol) and saturated aqueous sodium hydrogen carbonate (20 ml). The mixture was cooled to 0 C and a solution of sodium hypochlorite (42 ml, 1.2 M, 50.4 mmol) was added over a period of 1.5 hours. After stirring at 0°C for 30 minutes, the organic layer was separated and washed with NaHCO3 (aq) (3 x ml) and water (3 x 40 ml). To the combined water phases EtOH (50 ml) was added and the solution stirred for 30 minutes. EtOAc (100 ml) was then added and the pH of the mixture was brought from 9 to 2 by the addition of H-Dowex. The Dowex was filtered, the phases were separated and the water phase was extracted with EtOAc (4 x 100 ml), dried and concetrated under reduced pressure without warming to give (1.40 g, 3.25 mmol, 71 "C-NMR (CD3OD-acetone-d 6 2:1) 5 27.1 (CH3 isoprop), 73.7, 77.4, 78.0 111.0 (isoprop), 128.8, 129.1, 129.3 and 138.6 (aromatic 172.7 (COOH).

B Preparation of N1,N6-di[(1S)-2-methyl-l-(methylcarbamoyl)propyl]-(2R,3S,4S,5R)- 2,5-di(benzyloxy)-3,4-O-isopropyidene-3,4-dihydroxyhexanediamide.

The resultant crude product from step A (1.40 g, 3.25 mmol) was dissolved in dry CH 3

CN

(21 ml) under argon and pyridine (1.58 ml, 19.5 mmol) and di-N-succinylimidyl carbonate (DSC) (3.30 g, 12.9 mmol) was added. The mixture was stirred at room temperature for hours, diluted with EtOAc (50 ml), washed with water (3 x 20 ml) and brine (1 x 30 ml), dried, concentrated and purified by silica gel column chromatography.

The activated diacid from above (974 mg, 1.64 mmol) was dissolved in a 2:1 mixture of

CH

2

CI

2 -THF (7 ml) under argon and L-valine methylamide (560 mg, 4.30 mmol) was added.

The reaction was stirred at room temperature for 18 hours, diluted with CH 2 C12 (50 ml) and washed with NH 4 Cl (aq) (30 ml). The organic layer was dried, concentrated and purified by silica gel column chromatography (CHC13- MeOH 20:1) to yield 844 mg, 1.29 mmol, 79%.

27 'C-NMR (CDCI 3 5 16.7 and 19.7 (val 26.2 (val CH 3 NH), 27.2 (CH3 isoprop), 28.9 (val CHMe 2 57.8 (val CHNH), 73.7, 77.1, 77.0, 110.0 (isoprop), 128.2, 128.4, 128.7, 129.0 and 135.7 (aromatic 169.8 and 170.9 (CONH and val CO).

C Preparation of N ,N6-di[( S)-2-methyl- I -(methylcarbamoyl)propyl]-(2R,3S,4S,5R)- 2,5-di(benzyloxy)-3,4-dihydroxyhexanediamide.

The product from step B (775 mg, 1.18 mmol) was dissolved in a 5:1 mixture of CH CN-

H

2 0 (309 ml), 2,3-dichloro-5,6-dicyanobenzoquinone (DDQ) (30 mg, 0.13 mmol) was added and the temperature was kept at 75C. After 2 days and 4 days additional portions (2 x 60 mg) of DDQ were added and after 6 days the reaction was concentrated. The residue was dissolved in EtOAc (50 ml), washed with water (2 x 30 ml), filtered through a pad of charcoal, celite and Na 2 SO, and purified by silica gel column chromatography (CHCl 3 MeOH 20:1) to give (501 mg, 0.81 mmol, 69 [a]D 22.90 (c 1.4 g/100 ml CHCII).

"C-NMR (CDCI 3 5 16.7 and 19.7 (val CH 3 26.2 (val CH 3 NH), 28.6 (val CHNH), 57.7 (val CHNH), 69.6, 73.7, 77.6; 128.2, 128.9 and 135.9 (aromatic 170.8 and 172.3 (CONH and val CO). anal calcd. For C 32

H

46

N

4 0s: C 62.52; H 7.54; N 9.11. Found C 62.41; H 7.39; N 8.98.

D Preparation of N 1,N6-di[( 1S)-2-methyl-1 -(methylcarbamoyl)propyl]-(2R,3S,5R)- 2,5-di(benzyloxy)-3-hydroxyhexanediamide.

The product from step C (58 mg, 94 pmol) was dissolved in dry THF (5ml) under an argon atmosphere and heated to 45°C before N,N-thiocarbonyldiimidazle (40 mg, 0.22 mmol) was added. The reaction mixture was refluxed for 30 hours, concentrated and purified by silica gel column chromatography (chloroform-methanol 20:1) to give the thiocarbonate (60 mg, 91 pmol, 97 "C-NMR (CDC1 3 8 17.2 and 19.5 (val CH3), 26.4 (val CHNH), 29.7 (val CHMe 2 58.7 (val CHNH), 75.6, 78.0, 83.4, 128.3, 128.9 and 135.2 (aromatic 167.7 and 170.6 (CONH and val CO), 189.6 CS).

A suspension of the thiocarbonate (60 mg, 91 pmol), tributylin hydride (50 4l, 0.19 mmol) and a,a'-azaisobutyronitrile (15 mg, 91 tmol) in dry toluene (4 ml) was added dropwise to refluxing toluene (2 ml) over a period of 20 minutes. The mixture was refluxed for 28 minutes, allowed to cool and concentrated. The residue was dissolved in acetonitrile (10 ml) and washed with hexane (2 x 5 ml). The acetonitrile layer was concentrated and purified by silica gel column chromatography (toluene-acetone 1:1) to give the titled product in 33% yield (18 mg, 30 pmol). [a]D (c 0.7 g/100 ml CHC13).

3 C-NMR (CDC1 3 5 17.3, 17.4 and 19.6 (val CH 3 26.3 (val NHCH 3 29.5 (val CHMe 2 34.5 0 58.0, and 58.1 (val CHNH), 68,5, 72.7, 73.7, 76.8, 81.1, 127.9, 128.3, 128.5, 128.6, 128.8, 136.2 and 136.5 (aromatic 171.1, 171.4, 171.6, and 172.5 (NHCO and val

CO).

EXAMPLE 3 NI.N6-di fS1S)-2-methyl-l-(methvlcarbamovl)propvyl-(2R.3R.5R)-2.5-di(benzvloxy)-3hvdroxvhexanediamide.

The product of Example 1 (144 mg, 0.23 mmol) was dissolved in dry THF (8 ml) under an argon atmosphere and heated to 45 0 C before N,N-thiocarbonyldiimidazole (104 mg, 0.58 mmol) was added. The reaction mixture was refluxed for 17 hours, concentrated and purified by silica gel column chromatography (chloroform-methanol 20:1) to give the thiocarbonate (125 mg, 0.19 mmol, 83%).

3 C-NMR (CDC13): 8 17.4 and 19.4 (val CH 3 26.3 (val CH 3 NH), 30.2 (val CHMe2), 58.4 (val CHNH), 75.8, 77.7, 82.4 128.9, 129.1 and 135.3 (aromatic 167.0 and 170.6 (CONH and val CO)m 190.7 (CS).

The thiocarbonate (125 mg, 0.19 mmol) was suspended in dry toluene (4 ml) under an argon atmosphere and heated to reflux, and a solution of tributylin hydride (153 pl, 0.57 mmol) and ct,a'-azaisobutyronitrile (47 mg, 0.28 mmol) in dry toluene (3 ml) was added over a period of 10 minutes. The mixture was refluxed for 20 hours, allowed to cool and concentrated. The residue was dissolved in acetonitrile (15 ml) and washed with hexane (2 x 10 ml). The acetonitrile layer was concentrated and purified by silica gel column chromatography (CHCI3-MeOH 20:1) to give the titled product in 35% yield (40 mg, 67p.mol). [a]D (c 0.8 g/100 ml CHCI 3 '1C-NMR (CDCh): 5 17.5, 18.0, 19.4 and 19.6 (val CH3), 26.1 and 26.3 (val CH 3 NH), 29.5 and 30.6 (val CHMe2), 35.5, 58.2, 58.3, (val CHNH), 70.2, 73.0, 73.3, 77.2, 83.0, 127.9, 29 128.0, 128.4, 128.6, 136.7, (aromatic 170.5, 171.3, 121.4, 173.1 (CONH and val CO).

Anal calcd. For C 3 2

H

46

N

4 0 7 C 64.19; H 7.74; N 9.36; Found: C 64.00; H 7.50; N 9.15.

EXAMPLE 4 NL.N6-dif iS)-2-methyl-I-(methylcarbamobutyll-(2R3R.4R5R)-2-i enzlox-34dihvdroxvhexanediamide.

A solution of 2,5-di-O-benzyl-L-mannaric-1,4:6,3-di-lactone from Example 1 (50 mg) and N-methyl-L-isoleucine (120 mg) in acetonitrile was maintained at 70'C for 16 hours. After cooling the product was purified by silica gel column chromatography (CHCI 3 -MeOH 19:1) to give the pure compound.

'"C-NMR (CD30D) 5 11.7, 16.1, 25.1, 26.3, 37.2, 58.5, 72.3, 73.4, 80.8, 128.7, 129.1, 137.7, 172.7, 173.1.

EXAMPLE NI.N6-dir( IS)- 1 -(methylcarbamoyl)-2-phenylethyll-(2R.3R.4R.5R)-2.5-di(benzytvoxy)-34dihydroxvhexanediamide.

2,5-di-O-benzyl-L-mannaric-1,4:6,3-di-lactone from Example 1 (30 mg, 0.085 mmol) was dissolved in dichloromethane (3 ml) and phenylalanine-N-methylamide (91 mg, 0.51 mmol) was added to the stirred solution. The solution was refluxed (40 0 C) for 18 hours and then concentrated. The crude product was purified by silica gel column chromatography (chloroform-methanol 20:1).

"C-NMR (CD30D) 872.8, 73.7, 81.9, 127.1, 127.8, 128.4, 128.7, 128.8, 129.0, 135.0, 136.4, 170.0, 172.1.

EXAMPLE 6 N1,N6-dif 1S)-I -(methvlcarbamovl)-2-(4-hydroxphenl)ethyll-(2R3R4R,5R)-2,5di(benzvloxv)-3.4-dihvdroxyhexanediamide 2,5-di-O-benzyl-L-mannaric-1,4:6,3-di-lactone from Example 1(40 mg, 0.113 mmol) was dissolved in dichloromethane (4 ml) and tyrosine N-methylamide (132.2 mg, 0.677 mmol) was added to the stirred solution. The solution was refluxed (40 0 C) for 18 hours and concentrated. The crude product was purified by silica gel column chromatography (chloroform-methanol 20:1).

S

3 C-NMR (CD 3 OD) 5 26.1, 37.1, 55.3, 72.9, 72.9, 81.1, 116.1, 128.8, 129.2, 130.9, 138.3, 157.2, 172.9, 173.6.

EXAMPLE 7 N .N6-di[(1S)-2-methvl- 1 -(methylcarbamovl )propvll-(2R.3R4R.5R)-2,5-dif(4fluorobenzyvloxvy-3,4-dihydroxyhexanediamide.

A To a cooled (-10C) solution of 4-fluorobenzyl alcohol (0.85 ml, 8 1.168 g/ml, 7.93 mmol, leq) in CH 2 C1 2 (10 ml) and KOH (50% aq. 10 ml) was added tetrabutylammonium hydrogen sulphate (0.015 After stirring for 5 minutes, trichloroacetonitrile (0.953 ml, c 1.44 g/ml, 9.51 mmol, 1.2 eq) was added and the reaction mixture maintained at -5 0 C for minutes before warming to room temperature. The organic phase was separated and the water phase extracted with CH 2 C1 2 (2 x 10 ml). The combined extracts were dried with MgSO 4 and concentrated to one third. After filtration through Celite the solvent was removed in vacuo to give the dilactone as a brownish-yellow oil, 1.82 g (85% yield).

'H-NMR (CHCI 3 250 MHz) 8 5.30 2H), 7.00-7.15 2H), 7.40 2H), 8.40 1H).

B Preparation of 2,5-di-O-( 4 -fluorobenzyl)-L-mannaro-1,4:6,3-di-lactone.

To a solution of the dilactone from step A (0.200 g, 1.12 mmol) in dioxane (40 ml) was added 4-fluorbenzyl trichloroacetimidate (0.72g, c 1.353 g/ml 3.37 mmol, 3 eq). The reaction vessel was placed under a N 2 atmosphere and stirred (10 min). TMS-OTf (0.08 ml, c 1.230 g/ml, 0.45 mmol, 0.4 eq) was added portionwise. After 4 hours the mixture was filtered through a pad of SiO 2 -NaHCO3-SiO 2 recrystallised from CHCI 3 slurried with Et0O (2 ml) and filtered to give (0.315 g, 71.8% yield).

'H-NMR (DMSO-d 6 250 MHz) 8 4.75, 4.80 J=7.1 Hz, 4H), 4.90 J=3.7 Hz, 2H), 5.3 J=5.8 Hz, 2H), 7.15-7.25 4H), 7.40-7.55 4H). "C-NMR (DMSO-d 6 62.9 MHz) 71.4, 74.4, 74.7, 115.1, 115.5, 130.2, 130.3, 133.3, 160.0, 164.0, 171.8.

31 C To the benzylated di-lactone from above (0.05 0 g, 0.128 mmol, 1.0 eq) in CH 3

CN

(pa, 2 ml) was added L-valine-N-methylamide (0.050 g, 3.84 mmol, 3.0 eq). The reaction mixture was heated to 70 0 C and stirred for 24 hours. Concentration gave a brown oil (crude 0.107 purification by silica gel flash chromatography (9:1 CHCIl: MeOH) (12:1 CHCI 3 MeOH 1% HOAc) and (12:1 CHC1 3 MeOH) to give 0.006 g of the titled product (yield 'H-NMR (DMSO-d 6 250 MHz) 8 0.90-0.95 6H), 1.90-2.05 2H), 2.55 6H), 3.80- 3.90 2H), 3.95-4.05 J=7.8 Hz), 2H), 4.10-4.20 2H), 4.40-4.45 4H), 4.75-4.65 J=7.47 Hz, 2H), 7.10-7.20 4H), 7.25-7.40 4H), 7.75-7.80 J=8.94 Hz, 2H), 7.85-7.90 J=4.64 Hz, 2H). 3 C-NMR (DMSO-d 6 62.9 MHz) 8 18.0, 19.1, 25.3, 57.5, 69.6, 70.26, 79.2, 93.0, 114.7, 115.0, 129.5, 129.6, 134.1, 159.5, 163.4, 170.3, 171.0, 115.1, 115.5, 130.2, 130.3, 133.3, 160.0, 164.0, 171.8.

EXAMPLE 8 NI N6-dif( S)-2-methvl-1-(cyclopropylcarbamovl)propvl]l-2R,3R,4R.5R)-2.5di(benzvloxv)-3,4-dihydroxvhexanediamide.

An analogous procedure to Example 2B but using L-valine-N-cyclopropylamide (117 mg, 0.27 mmol) yielded the corresponding intermediate as an off-white solid (147 mg, 0.25 mmol, 93%).

3 C NMR (CDCI.): 8 6.1, 6.7 (CH2 cyclopropyl), 17.4, 19.4 (val CH 3 22.6 (val CHMel), 27.1 (CH 3 isoprop), 30.1 (CHNH-cyclopropyl), 57.8 (NHCH), 74.2 (C3, C4), 76.6 (C2, 78.9 (OCH2Ph), 110.3 (isoprop), 128.4, 128.7, 128.9, 136.5 (arom 169.3 (val C=O), 172.1 (NHC=O).

This intermediate (86 mg, 0.16 mmol) was subjected to the procedure of Example 2C to yield the titled compound (52 mg, 0.010 mmol, 65%) as an off-white solid. [ca] -17° (c.

0.86 CHCl).

'"C-NMR (CDC 1 6 5.8, 6.3 (CH 2 -cyclopropyl), 16.8, 19.5 (val CH3), 22.8 (val CHMe,), 28.9 (CHNH-cyclopropyl), 58.0 (NHCH), 73.4 (C3, C4), 73.4 (C2, C5), 82.2 (OCH 2 Ph), 128.6, 128.8, 129.3, 136.2 (arom 171.1 (val (172 NHC=O).

EXAMPLE 9 NI,N6-di(1 S)-2-methvl- -(methylcarbamovl)propvll-(2R,3R,4R,5R)-2.5-dif(2methylbenzvl)oxvl-3.4-dihydroxvhexanediamide.

A 1,6-di-(dimethyl-tert-butylsilyI)-3,4-O-isopropyl-L-mannitol (1.834 g, 4.07 mmol) was dissolved in dry tetrahydrofuran (25 ml). The solution was then cooled to 0°C and sodium hydroxide (60% aq. 0.556 g, 13.44 mmol, 3.3 eq) was added. The cooling bath was removed and the reaction stirred at room temperature for 15 minutes. 2-Methylbenzyl bromide (120 ml, 8.96 mmol 2.2 eq) and tetrabutylammonium iodide (0.285 g, 0.77 mmol, 0.19 eq) were added and the reaction mixture stirred at room temperature overnight. The solution was then diluted with diethyl ether (200 ml) and washed three times with water (200 ml). The organic phase was dried and concentrated. The product was purified by silica gel flash chromatography (toluene) to give the disilyl ether (1.86 g, 70 'H-NMR (CDC 3 250 MHz) 8 0.10 12H, 2x CH 3 C[CH3] 2 0.90 12H, 2x [CH3] 2 -Si), 1.30 6H, 2x CH3C[CH 3 2 1.40 6H, 2xisoprop, 2.30 6H, 2x Ph-CH3), 3.63-3.78 4H, H-l, 3.86-3.94 (dd, 2H, H-2, H-5, J=7.47, 3.20 Hz), 4.17-4.24 (dd, 2H, H-3, h-4, J=1.72, 1.29 Hz), 4.50-4.82 (dd, 4H, 2x O-CHz-Ph, J=52.55, 11.86 Hz), 7.08- 7.36 8H, 2x Ph).

"C-NMR (CDC13 62.9 MHz): 8 5.47 ([CH 3 2 18.61 (CH 3 C[CH3]2-Si), 25.90 (2x

CH

3 C[CH3] 2 27.25 (isoprop.C 29.72 (2xPh-CHJ), 63.70 71.38 78.29 (2xO-CH 2 81.47 109.49 (isoprop.C) 125.65, 127.42, 128.32, 129.31 (2xPh).

B Preparation of 2,5-di-O-(2-methylbenzyl)-3,4-isopropylidene-L-mannitol The disilyl ether from step A (1.749 g, 2.65 mmol) was dissolved in dry tetrahydrofuran.

Tetrabutylammoniumfluoride in THF (6.92 ml, 1.0 M, 2.6 eq) was added. The solution was stirred at room temperature for 2.5 hours and concentrated. The product was purified by silica gel flash chromatography (toluene: ethyl acetate 1:1) to give the titled diol (0.99 g, 88%).

'H-NMR (CDCI 3 250 MHz) 5 1,40 6H, 2x isoprop.CH 3 2.35 6H, 2x Ph-CH3), 3.60- 3.68 4H, H-I, 3.75-3.85 2H, H-2, 4.19-4.25 (dd, 2H, H-3, H-4, J=2.40, 1.35 Hz), 4.52-4.71 (dd, 4H, Ph-CH 2 J=13.49, 11.58 Hz), 7.10-7.25 8H, 2xPh).

33 "C-NMR (CDCI 3 62.9 MHz) 8 61.26 70.68 78.95 (2xO-CH 2 -Ph), 80.94 125.92, 127.47, 128.69, 129.32 (2x Ph).

C Preparation of 2,5- di-O-( 2 -methylbenzyl)-isopropylidene-L-mannaric acid To the diol from step B (0.126 g, 0.51 mmol) in dichloromethane (3.6 ml) was added 2,2,6,6-tetramethylpiperidin-1-yloxyl, free radical (0.003 g, 0.02 mmol, 0.03 eq) and a solution of potassium bromide (0.006 g, 0.05 mmol, 0.09 eq) and tetramethylammonium bromide (0.008 g, 0.03 mmol, 0.05 eq) and saturated aqueous sodium hydrogen carbonate (2.20 ml) was added. This solution was cooled to 0°C and a solution of sodium hypochlorite 4.32 ml, 10.2 mmol, 20 eq), saturated aqueous sodium chlorite (2.30 ml) and saturated aqueous sodium hydrogencarbonate (1.20 ml) was added over 45 minutes. After stirring for an additional 1 hour the organic phase was washed with 3x 15 ml H20 and the combined water phases were acidified with 1M hydrochloric acid to pH 2. The acid aqueous phase was then washed three times with ethyl acetate (15 ml), the organic phase was dried and concentrated to give the titles diacid. The product could be used directly in the next step.

D Preparation of N-(benzyloxycarbonyl)-N'-methyl-L-valinamide Cbz-valine (2.55 g, 10.17 mmol), methylamine hydrochloride (0.824 g, 12.20 mmol, 1.2 eq) and 1-hydroxybenzotriazole (2.06 g, 15.25 mmol, 1.5 eq) was dissolved in dichloromethane (125 ml) and triethyl amine (5.52 ml, 39.65 mmol, 3.9 eq) was added to adjust the pH to The solution was cooled to 0°C and N,N'-dicyclohexylcarbodiimide (2.94 g, 14.24 mmol, 1.4 eq) was added and the solution was stirred for an additional 1 hour in a cooling bath.

The solution was then stirred overnight at room temperature. The reaction was filtered, concentrated and dissolved in ethyl acetate (176 ml). The organic phase was washed with water (125 ml) and sodium hydrogen carbonate (125 ml) dried and concentrated. The product was purified by recrystallization in ethyl acetate-hexane to give the titled product (2.04 g, 'H-NMR (CDCI1, 250 MHz) 8 0.90-1.00 6H, [CH 3 2 CH-CH), 1.66 1H,

CH-CH[CH

3 2 3.80 3H, J=4.82 Hz), 3.88-3.99 IH, 5.10 2H, 5.25- 5.41 1H, NH-CO, J=7.67 MHz), 5.90-6.05 1H, NH-COO), 7.30-7.42 5H, Ph).

"C-NMR (CICI 3 69.2 MHz) 5 19.24 26.14 (CH-CH[CH 3 30.67 (CH-CH-[CH3]2), 60.67 67.06 128.02, 128.20, 128.54, 136.19 156.45 171.80 D (ii) Preparation of N-methyl-valinamide N-(benzyloxycarbonyl)-N'-methyl-L-valinamide from step above (2.49 g, 9.42 mmol) was dissolved in ethanol (100 ml) and palladium on activated carbon 0.399 g) was added. The mixture was then hydrogenated (ca. 230 ml H 2 at room temperature overnight.

The Pd/C was then filtered through a pad of Celite and concentrated. The product was purified with silica gel flash chromatography (dichloromethane-acetone 1:1) to give Nmethyl-valinamide (0.870 g, 71%).

'H-NMR (CDCI,, 250 MHz) 6 0.79-0.85 6H, CH-CH[CH 3 2 1.76 1H,

CH-CH[CH

3 2.74-2.76 2H, H-1, J=4.99 Hz), 3.75-3.77 1H, H-3, J=4.43), 6.76 (s, 2H, NH 2 7.26 1H, NH-CH3), 'C-NMR (CDCI3, 69.2 MHz) 6 19.68 26.23 (CH-CH[CH.] 2 30.77 (CH-CH-[CH3] 2 60.21 166.10 D (iii) Preparation of N1,N6-di[(1S)-2-methyl-l-(methylcarbamoyl)propyl]- (2R,3R,4R,5R)-3,4-O-isopropylidene-2,5-di[(2-methylbenzy])oxy]-3,4dihydroxyhexanediamide.

The diacid of step C (0.247 g, 0.56mmol) and the amide of step D (ii) (0.211 g, 1.62 mmol, 2.9 eq) were dissolved, together with 1-hydroxybenzotriazole (0.270 g, 2.00 mmol, 3.6 eq) in dichloromethane (5.8 ml) and tetrahydrofuran (2.9 ml). Triethylamine (0.18 ml, 1.28 mmol, 2.3 eq) was added to adjust the pH to 7.5. The reaction mixture was cooled to 0 C and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (0.287 g, 1.50 mmol, 2.7 eq) was added and the solution stirred in a cooling bath for 1 hour. The reaction mixture was then stirred for an additional 2 hours at room temperature. The reaction was then diluted with dichloromethane (30 ml) and washed with 3x30 ml sodium hydrogencarbonate, dried and concentrated. The product was purified by silica gel column chromatography (dichloromethane-methanol, 3:1) to give the titled diamide (0.291 g).

1H-NMR (DMSO-d 6 250 MHz) 5 0.78-0.94 12H, 2x (H-CH[CH3] 2 1.22 6H, isoprop.CH 3 1.87-1.93 2H, H-4, H-13), 2.24 6H, 2x Ph-CH 3 2.56 6H, H-l, H- 16, J=4.38 Hz), 4.11-4.14 6H, 2x CH-CH[CH 3 2 H-7, H-8, H-9, H-10), 4.46-4.50 4H, Ph-CH 2 J=9.92), 6.48-7.05 8H, Ph), 7.70-7.75 2H, 2x CH-NH-CO, J= 1.32 Hz), 8.00 2H, 2x CH3-NH, J=4.44).

E Preparation of N1,N6-di[(l S)-2-methyl- 1-(methylcarbamoyl)propyl]- (2R,3R,4R,5R)-2,5-di[(2-methylbenzyl)oxy]-3,4-dihydroxyhexanediamide.

Hydrochloric acid, 45 in methanol (2 ml) was added to the diamine end product of step D (0.060 g, 0.090 mmol). The reaction mixture was stirred at room temperature for 60 minutes and then concentrated. The product was purified by silica gel column chromatography (dichloromethane: methanol 9:1) to give the titled diamide (0.042 g 74%).

1H-NMR (DMSO-d 6 250 MHz) 5 0.82-0.87, 12H, 2x CH-CH[ CH312, J=6.06), 1.94-2.02 2H, CH-CH[CH3]2), 2.25 6H, 2x Ph-CH 3 2.60 6H, H-I, H-16, J=4.42), 3.86-3.88 2H, H-4, H-13), 4.01-4.04 2H, H-8, H-9, J=7.27), 4.18-4.19 2H, H-7, H-10), 4.47 4H, Ph-CH 2 7.15-7.31 8H, Ph), 7.70-7.74 2H, 2x CH-NH-CO, J=8.82), 7.90- 7.92 2H, 2x CH 3 -NH, J=4.49).

3 C-NMR (DMSO-d 6 69.2 MHz) 5 17.99 CH-CH[ CH 3 19.13 (Ph-CH 3 25.31 (CH-CH[

CH

3 2 30.40 C-16), 57.57 C-13), 69.37 (Ph-CH2-O), 69.80 C-10), 79.29 125.47, 127.59, 128.30, 129.78, 131.48, 136.42 170.38 C-14), 172.03 C-11).

EXAMPLE NI.N6-di f[(1S2R)-2-hvdroxv-2,3-dihydro- 1 H-I -indenyll-(2R.3R.4R.5R)-2.5-di(benzvl oxv)- 3.4-dihydroxvhexanediamide.

0.915 g (2.2 eq., mmol) of(lS,2R)-l-amino-2-indanol was suspended in 12 ml CHC1 3 and heated to 45 0 C. 2,5-di-O-benzyl-L-mannaric-1,4:6,3-di-lactone (1.0 g, 1.0 eq., 2.82 mmol) from Example 1 was added all at once and stirring was continued at 50°C during 4 h.

Subsequently, the reaction mixture was extracted with saturated aqueous NH 4 CI (2x) and water dried with MgSO 4 and concentrated. Recrystallization from methanol gave (0.65 g, 35%) as a white solid.

"C NMR (CDCl 3 5 39.2, 57.8, 71.6, 72.5, 73.5, 81.5, 124.0, 125.3, 127.0, 128.2, 128.3, 128.6, 136.7, 139.8, 140.8, 171.6. Anal. (C 3

I

8

H

4

N

2 0) C, H, N. [ot]D-20.7 (c 0.68, CHCI 3 EXAMPLE 11 NI-[(1 S2R)-2-hvdroxv-2,3-dihvdro-IH-1-indenyll-N6-( S)-2-methvI-]- (methvlcarbamoyl)propyll-(2R.3R.4R.5R)-25-di(benzyloxy)-3.4-dihydroxyhexanediamide.

208 mg (1.0 eq. 1.41 mmol) of (1S,2R)-l-amino-2-indanol was suspended in 4 ml CH 3

CN

and heated to 55°C. 2,5-Di-O-benzyl-L-mannaric-1,4:6,3-di-lactone (0.5 g, 1.0 eq., 1.41 mmol) from Example I was added all at once and stirring was continued at 50 0 C during h together with 1 ml CH 3 CN. After 3.5 h another portion of. L-valine methylamide (184 mg, eq. 1.41 mmol) was added. After 22 h the mixture was concentrated and purified with silica gel flash chromatography (CHCI-MeOH 30:1; CHCI 3 -MeOH 20:1 and EtOAc-MeOH 10:1) to give (14 mg, 'C NMR (CDCI3) 8 17.2, 19.5, 39.3, 58.0, 58.4, 72.2, 72.4, 72.8, 73.4, 81.4, 81.8, 123.9, 125.4, 127.0, 128.1, 128.2, 128.4, 128.5, 128.7, 128.8, 136.4, 139.6, 140.8, 170.9, 172.0.

EXAMPLE 12 N1 N6-dif(1 S,2R)-2-hvdroxv-2.3-dihydro-1 H-l -indenvly-(2R.3R,4R,5R)-2.5-di(2fluorobenzvloxy)-34-dihydroxyhexanediamide.

A Preparation of 2-fluorobenzyl trichloroacetimidate.

Procedure as described in Example 7A but using 2-fluorobenzylalcohol gave (2.79 g, 74%).

IH-NMR (CDCL 3 250 MHz) 5 5.36 2H, 7.23-7.49 6H, Ph), 8.45 1H, NH) 13 C-NMR (CDCL 3 62.9 MHz) 6 64.65 91.14 115.14, 115.45, 123.97, 128.90, 129.97, 130.11 162.18 B Preparation of 2,5-di-O-(2-fluorobenzyl)-L-mannaric-l,4:6,3-dilactone.

Procedure as described in Example 7B but using 2-fluorobenzyl trichloroacetimidate gave (0.303 g, 73%) as a white solid.

1 H-NMR (DMSO-d 6 250 MHz) 5 4.83 4H, 2x Ph-CH 2 J=8.00), 4.93 2H, H-3, H-4, J=3.72), 5.31 2H, H-2, H-5, J=3.78), 7.19-7.54 8H, 2x Ph) 1 3 C-NMR (DMSO-d 6 62.9 MHz) 5 65.78 74.22 74.95 (2x Ph-CH 2 115.04, 115.37, 124.41, 130.25, 130.38, 130.58 (2x Ph), 170.41 C-6).

37 C Prepared by the procedure as described in Example 10 but using 2,5-di-O-(2fluorobenzyl)-L-mannaric-1,4:6,3-dilactone gave the title compound (0.056 g, 33%).

1 H-NMR (DMSO-d 6 250 MHz) 5 2.77-3.11 (d+dd, 4H-, H-l, H-14, J=4.58, 11.56, 16.07), 3.96 2H, H-3, H-12, J=7.28), 4.16 2H, H-7, H-8, J=7.69), 4,44 2H, H-6, H-9, J=3.83, 4.43), 4.62 4H, 2x Ph-CH 2 J=3.40), 4.92 (d 2H, 2x OH-CH, J=7.23), 5.08 (d, 2H, CH 2 -OH, J=4.10), 5.27 2H, C-2, C-13, J=3.61, 5.53), 7.11-7.51 16H, 4x Ph), 7.82 2H, NH, J=8.72) 13 C-NMR (DMSO-d 6 62.9 MHz) 8 54.79 C-14), 56.57 (C- 3, C-I2), 64.88 (2x Ph-CH 2 69.81 72.01 C-13), 79.53 114.80, 115.15, 124.18, 125.70, 124.91, 126.09, 127.16, 129.55, 129.68, 130.10, 140.55, 141.90, 157.86, 161.76 (4x Ph) 170.64 EXAMPLE 13 N 1.N6-di[(l S.2R)-2-hydroxy-2,3-dihydro- 1H-1-indenyll-(2R,3R.,4R,5R)-2.5-di(4fluorobenzvloxv)-3,4-dihvdroxvhexanediamide A Preparation of4-fluorobenzyl trichloroacetimidate.

Procedure as described in Example 7A but using 4-fluorobenzylalcohol gave (3.036 g, 81%).

1 H-NMR (CDCL 3 250 MHz) 5 5.29 2H, 7.18-7.43 6H, Ph), 8.45 1H, NH) 13 C-NMR (CDCL 3 62.9 MHz) 5 70.02 91.35 115.32, 115.67, 129.77, 129.90, 131.26, 162.40 B Preparation of 2,5-di-O-(4-fluorobenzyl)-L-mannaric-l,4:6,3-dilactone.

Procedure as described in Example 7B but using 4-fluorobenzyl trichloroacetimidate gave (0.242 g, 54%) as a white solid.

1 H-NMR (DMSO-d 6 250 MHz) 8 4.74 4H, 2x Ph-CH2, J=6.87), 4.87 2H, H-3, H-4, J=4.00), 5.24 2H, H-2, H-5, 7.18-7.47 8H, 2x Ph) 13 C-NMR (DMSO-d 6 62.9 MHz) 6 71.17 74.25 74.68 (2x Ph-CH 2 114.92, 115.27, 130.00, 130.13, 133.07, (2x Ph), 171.54 C-6).

C Prepared by the procedure as described in Example 10 but using 2,5-di-O-(4fluorobenzyl)-L-mannaric-1,4:6,3-dilactone gave the title compound. (0.053 g, 1 H-NMR (DMSO-d 6 250 MHz) 82.78-3.11 (d+dd, 4H, H-1, H-14, J=4.63, 11.53, 16.08), 3.95 2H, H-3, H-12, J=7.64), 4.11 2H, H-7, H-8, J=8.04), 4,45 2H, H-6, 4.51 4H, 2x Ph-CH 2 J=6.02), 4.89 (d 2H, 2x OH-CH, J=7.45), 5.10 21-1, CH 2

-OH,

J=4.16), 5.27 2H, C-2, C-13, J=3.59, 4.96), 7.09-7.84 161H1, 4x Ph), 8.30 2H, NH, J=8.74) 13 C-NMR (DMSO-d 6 62.9 MHz) 6 54.80 C-14), 56.56 C-12), 69.72 (2x Ph-CH2), 70.38 72.02 C-13), 79.36 114.68, 115.01, 124.23, 124.71, 126.09, 127.18, 129.62,129.74, 134.15, 140.56, 141.92, 159.55, 163.40 (4x Ph), 170.81 EXAMPLE 14 NLN6-dif IS)-2-methl- -(methvicarbamovl)propyll-(2R3R,4R,5R)-2,5-dif(4 phenylbenzylloxvy-3.4-dihvdroxvhexanediamide.

A Preparation of 4-bromobenzyl trichloroacetimidate.

Procedure as described in Example 7A but using 4-bromobenzylalcohol gave (3.2 g, 1 H-NMR (CDCL 3 250 MHz) 8 5.3 2H), 7.3 7.5 8.4 1 3 C-NMR (CDCL 3 62.9 MHz) 8 69.84, 122.32, 129.40, 131.65, 134.38, 162.32.

B Preparation of 2,5-di-O-(4-bromobenzyl)-L-mannaric- 1,4:6,3-dilactone.

Procedure as described in Example 7B but using 4-bromobenzyl trichloroacetimidate gave (0.739 g, 83%).

1 H-NMR (DMSO-d 6 250 MHz) 6 4.7 4.8 4.9 5.3 7.4 7.6 1 3 C-NMR (DMSO-d 6 62.9 MHz) 871.27, 74.40, 74.92, 130.08, 131.36, 136.44, 171.68.

C Preparation of NI,N6-di[(1 S)-2-methyl-1 -(methylcarbamoyl)propyl]- (2R,3R,4R,5R)-2,5-di[(4-bromobenzyl)oxy]-3,4-dihydroxyhexanediamide.

Procedure as described in Example 10 but using 2,5-di-O-(4-bromobenzyl)-L-mannaric- 1,4:6,3-dilactone gave (0.0746 g, 66%).

13 C-NMR (DMSO-d 6 62.9 MHz) 8 17.93, 19.81, 26.32, 30.84, 58.93, 72.11, 72.51, 80.72, 122.67, 130.36, 132.29, 136.80, 172.35, 172.88.

39 D Preparation of the title compound. The product from above (30 mg, 0.039 mmol), phenylboracid (23.6 mg, 0.194 mmol), Pd(PPh 3 4 (2.2 mg, 0.0019 mmol), NaCO 3 (77.6 1, 2M), EtOH (60 pl), H 2 0 (80 and dimethoxyethane (240 pl) was mixed in a reaction tube fitted with screw caps in an atmosphere of N 2 The tube was subjected to microwave irradiation for 4 min at 45 W. The tube was cooled in water before the reaction mixture was concentrated and purified by silica gel column chromatography (CHCl3-MeOH 20:1) to give 28 mg.

13 C-NMR (CDCI 3 62.9 MHz) 5 17.08, 19.79, 26.18, 29.04, 58.42, 73.28, 73.64, 81.96, 127.20, 127.68, 128.96, 129.01, 135.26, 140.51, 141.71, 170.55, 172.53.

EXAMPLE NL.N6-dir( 1S)-2-methvl- -(methvlcarbamovl)propvll-(2R,3R.4R,5R)-2,5-di4-(3thienvl)benzvloxvl-3,4-dihvdroxyhexanediamide.

Preparation of the title compound. The product from Example 14C above (33 mg, 0.043 mmol), 3-thipheneboracid (23.9 mg, 0.214 mmol), Pd(PPh 3 4 (2.5 mg, 0.00214 mmol), NaCO. (85 pl, 2M), EtOH (65 H 2 0 (90 p and dimethoxyethane (270 pl) was mixed in a reaction tube fitted with screw caps in an atmosphere of N 2 The tube was subjected to microwave irradiation for 4 min at 45 W. The tube was cooled in water before the reaction mixture was concentrated and purified by silica gel column chromatography (CHCI-MeOH 20:1) to give 30 mg.

13 C-NMR (CDCIl/MeOH-d 4 62.9 MHz) 8 16.55, 18.42, 24.91, 29.54, 57.64, 70.96, 71.77, 79.38, 119.70, 125.34, 125.63, 125.76, 128.00, 135.14, 141.08, 171.32, 171.66.

Anal.calcd. C, 61.61; H, 6.47; N, 7.19. Found: C, 61.2; H, 6.5; N 7.2.

EXAMPLE 16 N1.N6-dir(IS)-] -phenyl-1-(methvlcarbamovl)methyl1-(2R,3R,4R.5R)-2.5-di(benzvloxv)- 3.4-dihvdroxvhexanediamide.

2,5-di-O-benzyl-L-mannaric-l,4:6,3-di-lactone from Example 1 (150 mg, 0.423 mmol) was dissolved in acetonitrile (1 ml) and phenylglycine-N-methylamide (271 mg, 1.67 mmol) was added to the stirred solution. The solution was heated to 65°C for 14 hours and then concentrated. The crude product was purified by silica gel column chromatography (chloroform-methanol 9:1).

"C-NMR (CD 3 0D and CDCI 3 5 26.3, 26.4, 57.1, 71.8, 73.4, 80.3, 127.4,128.3, 128.4, 128.5, 128.6, 129.0, 136.5, 137.2, 170.3, 171.3.

Anal.calcd. C, 66.85; H, 6.20; N, 8.21. Found: C, 66.74; H, 6.34; N 8.12.

EXAMPLE 17 N1.N6-dir(lS)-2-methyl-l-(methvlcarbamovl)propvyl-(2R.3R.4R.5R)-2.5-di[4-(3fluorobenzvl)oxvl-3,4-dihydroxyhexanediamide.

A Tetrabutyl ammonium hydrogen sulphate (30 mg, 0.09 mmol) was added to 3fluorobenzylalkohol (1.6 g, 12.4 mmol) in CH2Cl2 (15 ml) and KOH 15 ml). This mixture was cooled to -15 0 C prior to the addition of trichloroacetonitrile (1.5 ml, 14.9 mmol). After 30 min at -10 0 C and 2.5 h at ambient temperature the phases were separated.

The aqueous phase was extracted twice with CH 2 Cl 2 The combined organic phases was dried with MgSO 4 and purified with silica gel column chromatography (toluene) to give 3fluorobenzyl trichloroacetimidate (3.27 g, 95%) as a clear oil.

1H-NMR (CDC1 3 8 5.3 7.0-7.15 7.3-7.45 8.4 B Preparation of 2 ,5-di-O-(3-fluorobenzyl)-L-mannaric- 1,4:6,3-dilactone.

Procedure as described in Example 7B but using 3-fluorobenzyl trichloroacetimidate gave (0.220 g, 33%) as a white solid.

1 H-NMR (DMSO-d 6 250 MHz) 6 4.8 4H, 4.9 2H, 5.25 2H, 7.15-7.25 8H), 7.3-7.35 2H). 1 3 C-NMR (DMSO-d 6 62.9 MHz) 8 71.08, 74.27, 74.67, 114.45, 114.80, 123.59, 130.30, 130.53, 139.8, 171.55.

C Prepared by the procedure as described in Example 7C, but using 2,5-di-O-(3fluorobenzyl)-L-mannaric-1,4:6,3-dilactone gave the Nl,N6-di[(1S)-2-methyl-1- (methylcarbamoyl)propyl]-(2R,3R,4R,5R)-2,5-di[4-(3-fluorobenzyl)oxy]-3,4dihydroxyhexanediamide 0 .030 g, 16%) as a white solid.

EXAMPLE 18 NI.N6-di[(lS.2R)-2-hvdroxv-2.3-dihvdro-lH-1-indenvll-(2R.3R,4R.5R)-2.5-di(3fluorobenzvloxv)-3,4-dihydroxvhex anediamide Prepared by the procedure as described in Example 10 but using 2,5-di-O-(3-fluorobenzyl)- L-mannaric-l,4:6,3-dilactone from Example 17B (0.03g, 0.08 mmol) and (IS,2R)-l-amino- 2-indanol (0.046 g, 0.31 mmol) gave the title compound (0.04 g, 76%).

1 3 C-NMR (DMSO-d 6 62.9 MHz) 5 39.4, 56.6, 69.7, 70.2, 72.0, 79.5, 113.8, 113.9, 114.2, 114.3, 123.2, 124.2, 124.7, 126.1, 127.2, 130.0, 130.1, 140.6, 141.0, 141.1, 141.9, 160.1, 164.0, 170.7.

EXAMPLE 19 Nl.N6-dif(1S)-2-methvIl--(methvlcarbamoyl)propyll-(2R,3R,4R,5R)-2.5-di[4-(2fluorobenzvl)oxy]-3,4-dihydroxyhexanediamide.

The procedure as described in Example 7C, but using 2,5-di-O-(2-fluorobenzyl)-Lmannaric-l,4:6,3-dilactone (Example 12B) gave the title compound. (0.062 g, 38%) as a white solid.

13 C-NMR (DMSO-d 6 62.9 MHz) 17.90., 19.11, 25.29, 30.43, 57.49, 64.78, 69.74, 79.38, 114.82, 115.15, 124.18, 124.59, 129.59, 130.14, 170.14, 170.93.

EXAMPLE N .N6-difl1S)-2-methyl- 1-(methvlcarbamovl)propvll-(2R3R,4R.5R)-2.5-di[4-(2.4difluorobenzvloxvy-3,4-dihydroxvhexanediamide.

A Tetrabutyl ammonium hydrogen sulphate (15 mg) was added to 2,4difluorobenzylalcohol (1.0 g, 6.94 mmol) in CH 2

C

2 (10 ml) and KOH 10 ml). This mixture was cooled to -15"C prior to the addition of trichloroacetonitrile (1.2 ml, 8.32 mmol). After 30 min at -15°C and 3 h at ambient temperature the phases were separated.

The aqueous phase was extracted twice with CH 2

C

2 The combined organic phases were dried with MgSO 4 and reduced to one third. The residue was filtered through celite and concentrated to give the crude 2,4-difluorobenzyl trichloroacetimidate (1.83 g, 92%) as a yellow oil.

IH-NMR (CDC 3 5 5.35 6.75-6.90 7.50 8.50 13 C-NMR (CDC 3 62.9 MHz) 5 64.1, 91.1, 103.9, 111.1, 118.6, 131.2, 158.9, 161.0, 162.3, 162.9, 165.2.

B Preparation of 2,5-di-0-(2,4-difluorobenzyl)-L-mannaric- 1, 4 :6,3-dilactone.

Procedure as described in Example 7B but using 2,4-difluorobenzyl trichloroacetimidate gave (0.97 g, 99%) the titled benzylated dilactone-as a white solid.

IH-NMR (DMSO-d 6 250 MHz) 5 4.75 4H, 1=2.1, 5.20 4H, 6.9-7.1 (m, 4H), 7.5-7.65 21-H).

C .The benzylated dilactone from above (0.11g, 0.28 mmol) and N-mnethyl-L-valine (0.22 g, 1.66 mmol) were refluxed in dichioromethane (1 ml) overnight. Concentration and purification by silica gel column chromatography (dichloromethane-methanol 9:2) gave the title diamide (0.042 g, as a white solid.

13 C-NMR (MeOH-d 4 62.9 MHz) 5 18.4, 19.7, 26.2, 31.2, 59.9, 66.8, 72.1, 81.4, 104.2, 104.6, 104.9, 112.0, 112.4, 121.7, 122.1, 133.1, 133.2, 173.3, 173.8.

EXAMPLE 21 NI, N6-dilllS2R1-2-hydroxy-2,3-dihydro-1H-1-indenvll- 2R,3R4R,5R)-2,5-di(2,4difluorobenzvloxv)-3.4-dihydroxvhexanediamide Procedure as described in Example 20C but using (IS,2R)-1-amino-2-indanol (0.16 g, 1.09 mmol) gave the title compound (0.048 g, as a white solid.

IH-NMR (MeOH-d 4 62.9 MHz) 5 2.90 (2d, 2H), 3.15 (2d, 2H), 4.20 2H), 4.62 4H), 4.7 2H), 5.4 2H), 6.8-7.0 4H), 7.2-7.4 8H), 7.45-7.65 6H).

EXAMPLE 22 NI ,N6-dilf(IS)-2-methyl--(methycarbamovl)Dpropll-(2R,3R,4R.5R)-2,5-dif4-(2pyridvl)benzvloxl-34-dihvdroxyhexanediamide.

43 The product from Example 14C above (30 mg, 0.039 mmol), trimethyl-2-pyridyl tin (47.0 mg, 0.194 mmol), Pd(PPh 3 )4 (2.25 mg, 0.0010 mmol), DMF (1 ml) and CuO (3.1 mg, 0.039 mmol) were mixed in a reaction tube fitted with screw caps in an atmosphere of N 2 The tube was subjected to microwave irradiation for 2 min at 60 W. The mixture was diluted with CH 2

C

2 (50 ml), washed with brine (3 x 20 ml), concentrated and purified by silica gel column chromatography (CH 2 Cl 2 -MeOH 15:1) to give 16 mg of the title compound.

1 3 C-NMR (CDC 3 /MeOH-d 4 62.9 MHz) 8 18.6, 20.0, 26.4, 31.9, 60.1, 72.3, 73.2, 81.6, 122.7, 123.9, 128.3, 129.7, 139.1, 140.1, 150.4, 158.7, 173.7, 174.1.

EXAMPLE 23 NI.N6-di(l S)-2-methyl- I-(methylcarbamovl)propvll-(2R,3R.4R,5R)-2.5-di[4-2pvridvl)benzvloxvl-3 4-dihydroxvhexanediamide.

The same procedure as for Example 22 above but using trimethyl-3-pyridyl tin (47.0 mg, 0.194 mmol) and purified by silica gel column chromatography (CHCI 3 -MeOH 9:1) gave the title compound, 15 mg.

13 C-NMR (CDCl 3 /MeOH-d 4 62.9 MHz) 5 18.1, 20.0, 31.1, 59.2, 72.4, 73.0, 81.0, 125.1, 128.1, 129.7.

EXAMPLE 24 N1.N6-dif( 1S)-2-methl- 1-(methvlcarbamovl)propvl-(2R.3R.4R.5R12.5.di(4-(3nitrophenvl)benzyl)oxyl-3.4-dihydroxvhexanediamide.

The product from Example 14C above (200 mg, 0.259 mmol), 3-nitrophenylboracid (216.1 mg, 1.294 mmol), Pd(PPh 3 4 (15.0 mg, 0.0129 mmol), NaCO 3 (259 l, 2M), EtOH (773 gl), (1288 p1) and dimethoxyethane (3091 pi) were mixed in a reaction tube fitted with screw caps in an atmosphere of N 2 The tube was subjected tto 80 °C overnight. The mixture was diluted with CHCI 2 (50 ml), washed with brine (3 x 20 ml), concentrated and purified by silica gel column chromatography(CHCl 3 -MeOH 20:1) to give 206 mg of the title compound.

13 C-NMR (DMSO-ds, 62.9 MHz) 5 18.1, 19.2, 25.4, 30.5, 57.6, 69.8, 70.7, 79.5, 120.9, 122.0, 126.8, 128.4, 130.4, 133.1, 137.0, 138.5, 141.4, 148.4, 170.0, 171.1.

EXAMPLE N1IN6-di [(1S)-2-methyl-1 -(methvlcarbamoyl)propvll-(2R.3R.4R.5R)-2,5-di 4-(2thienvl)benzvloxvl-3,4-dihvdroxvhexanediamide.

The product from Example 14C above (100 mg, 0.129 mmol), 2-thiophene boracid (82.8 mg, 0.647 mmol), Pd(PPh 3 4 (7.49 mg, 0.0065 mmol), NaCO 3 (129 1l, 2M), EtOH (386 (644 ul) and dimethoxyethane (1546 l) were mixed in a reaction tube fitted with screw caps in an atmosphere of N 2 The tube was subjected 80 °C overnight. The mixture was diluted with CHC1 (50 ml), washed with brine (3 x 20 ml), concentrated and purified by silica gel column chromatography(CHC 3 -MeOH 20:1) to give 88.8 mg of th title compound.

13 C-NMR (CDCl 3 /MeOH-d 4 62.9 MHz) 5 17.0, 18.3, 19.6, 26.0, 29.0, 50.7, 58.3, 72.9, 73.0, 81.4, 123.4, 125.2, 126.2, 128.1, 128.6, 131.9, 134.6, 135.3, 143.5, 170.7, 172.1.

Anal. Found: C, 68.9; H, 6.33; N 7.36.

EXAMPLE 26 N1 N6-di(SS.2R)-2-hvdroxv-2,3-dihvdro-I H-1-indenvll-(2R.3R.5R1-2.5-di(benzvloxv)-3hvdroxvhexanediamide.

A To a stirred solution of the product from Example 10 above (400 mg, 620 mmol) in

CH

2 C2 (3 ml) at 0 "C under an argon atmosphere was added lutidine (142 pl, 1.22 mmol) and t-butyldimethylsilyl triflate (296 pi, 1.29 mmol). After 4 h, IM NaOH (0.5 ml) was added and the mixture was washed with IM HC1 and brine. The organic layer was dried, concentrated and purified by silica gel column chromatography (CHC1 3 -MeOH 80:1) to give the disilylated compound (313 mg, 355 pmol, 57%).

"C NMR (CDC 3 6 17.9, 25.7, 40.6, 56.3, 71.1, 74.0, 77.2, 124.5, 124.8, 126.9, 127.9, 128.2, 128.5, 136.7, 139.8, 140.9, 176.2.

B To a solution of the product from step a) above (922 mg, 1.05 mmol) in CH 2 C1 2 (21 ml) was added NN-thiocarbonyldiimidazole (598 mg, 3.36 mmol). The mixture was refluxed for 22 h, concentrated and purified by silica gel column chromatography (toluene- EtOAc 3:1) to give (887 mg, 0.961 mmol, 92%) of the intermediate 'C NMR (CDCI 3 5 17.8, 25.6,40.5, 56.9, 74.3, 75.2, 78.2, 82.7, 124.6, 124.8, 126.6, 128.6, 128.8, 129.0, 135.6, 140.0, 141.5, 165.7, 192.0.

C To a refluxing solution of the product from step b) above in toluene (84 mi) under an argon atmosphere was added, a solution of tributyl tin hydride (0.70 ml, 2.6 mmol) and a,c'-azoisobutyronitrile (284 mg, 1.73 mmol) in toluene, over a period of 20 min. After 20 h additional tributyl tin hydride (0.23 ml) and a,oa'-azoisobutyronitrile (71 mg) was added.

After another 4 h the mixture was concentrated, dissolved in toluene and washed with 2.5 M NaOH and water, dried and concentrated. The residue was purified by silica gel column chromatography (toluene-EtOAc 3:1) to give (150 mg, 0.17 mmol, "C NMR (CDC13) 5 18.0, 25.7, 36.4, 40.5, 40.6, 56.3, 56.4, 69.8, 73.3, 74.0, 74.5, 82.0, 124.5, 124.7, 124.8, 126.9, 127.0, 127.1, 127.7, 127.9, 128.0, 128.2, 128.3, 128.4, 128.5, 136.7, 139.7, 141.2, 141.3, 171.3, 173.4.

D To a stirred solution of the product from step c) above (150 mg, 0.17 mmol) in MeOH (6 ml) was added H'-Dowex. After 3 days at ambient temperature the mixture was filtered, concentrated and purified by silica gel column chromatography (CHCI 3 -MeOH 20:1), followed by recrystallisation from MeOH, to give N1,N6-di[(lS,2R)-2-hydroxy-2,3dihydro-1H- -indenyl]-( 2

R,

3 R,5R)-2,5-di(benzyloxy)-3-hydroxyhexanediamide (38 mg, 0.060 mmol, 34%).

"C NMR (CDCI.-MeOH-d 6 8 35.9, 39.8, 39.9, 57.2, 57.3, 69.2, 72.5, 72.7, 73.4, 74.0, 77.6, 84.0, 124.1, 124.2, 125.5, 127.2, 128.3, 128.4, 128.5, 128.8, 137.1, 140.2, 140.4, 140.6, 140.7, 171.4.

EXAMPLE 27 NI-( 1 S,2R)-2-hydroxy-2,3-dihydro-H- 1-indenvll-N6-(2-chloro-6-flu orobenzyl)- (2R.3R.4R,5R,)-2,5-di(2-fluorobenzvloxy)-3,4-dihydroxyhexanediamide.

A Preparation of (lS,2R)-1-phtalimido-2-indanol on solid support.

N

0 0 Polymer Polymer Polymer Dry Merrifield resin with a dihydropyran linker (300 mg, 2.1 mmol/g, 0.65 mmol linker) was swollen in 1,2-dichloroethane (4.2 ml) under an argon atmosphere. Anhydrous PPTS (300 mg, 1.2 mmol) and (1S,2R)-l-phtalimido-2-indanol (501 mg, 1.8 mmol) were added and the mixture was heated to 75 After 20 h the mixture was cooled to room temperature and the solid was rinsed with CH 2 C2 (20 ml), THF (10 ml) and dried under vacuum.

B Preparation of (1S,2R)-l-amino-2-indanol on solid support.

Polymer -J Methylamine in ethanol (4.0 ml, 33%) was added to the solid material from the previous step and stirred for 16 h. The resulting solid was rinsed with CH 2 C1 2 (10 ml), THF (10 ml), MeOH (10 ml) and CHCI 3 (10 ml) and dried under a vacuum.

C Preparation of N I-(2,5-di-O-(2-fluorobenzyl))-1,4-mannolactone-6-amide-N6- [(1S,2R)-2-hydroxy-2,3-dihydro- 1H-1-indenyl] on solid support.

Polymer The solid material from the previous step was swollen in 1,2-dichloroethane (4.5 ml) for minutes under an argon atmosphere. 2,5-Di-O-(2-fluorobenzyl)-L-mannaric- 1,4:6,3dilactone (Example 12B) (502 mg, 1.29 mmol) was added and the mixture was kept at 50 °C for 16 h before it was allowed to cool and rinsed with CH 2

CI

2 (10 ml) and CHCI 3 (1 0ml).

D Preparation of S,2R)-2-hydroxy-2,3-dihydro-l H-1-indenyl]-N6-[2-chloro-6fluorobenzyl]-(2R,3R,4R,5R,)-2,5-di(benzyloxy)-3,4-dihydroxyhexanediamide on solid support.

Polymer The solid material from the previous step was swollen in CH3CN (4.5 ml) for 40 m under an argon atmosphere. 2-Chloro-6-fluorobenzylamine (249 mg, 1.56 mmol) was added and the mixture was kept at 50 °C for 16 h before it was cooled and rinsed with CH 2

C

2 (10 mi) and

CHCI

3 (10 ml).

E Preparation of N1-[(1S,2R)-2-hydroxy-2,3-dihydro-1 H-1 -indenyl]-N6-(2-chloro-6fluorobenzyl)-(2R,3R,4R,5R,)-2,5-di(2-fluorobenzyloxy)-3,4-dihydroxyhexanediamide.

FF

SNO HO o0 N N N HO 0 OH o Cl

F

2.3 M HCI in methanol (8.5 ml) was added to the solid material from the previous step.

After 4 h in an atmosphere of argon the solid was rinsed with CH 2 C12 (20 ml) and MeOH ml). The combined organic layers were collected and combined and diluted with CHCIS (20 ml) and washed with sat. Aqueous NaHCO 3 (2 x 30 ml). The combined aqueous layers was extracted with CHCl 3 (2 x 40 ml). The combined organic layer was concentrated and purified with silica gel chromatography (CHCIr-MeOH 40:1) to give the title compound (68 mg, 23 yield based on the loading).

"C NMR (CDC13) 34.3, 34.3, 39.2, 57.8, 67.4, 68.2, 71.3, 71.5, 72.4, 72.5, 79.8, 80.9, 114- 140, 159.1, 159.2, 159.7, 162.8, 162.9, 163.4, 171.2, 172.1.

EXAMPLE 28 N 1-[(1S.2R)-2-hvdroxv-2.3-dihvdro-1 H- 1-indenvll-N6-(1 S)-2-methvl-1 Dvridvlmethvllcarbamovl)propvll-(2R.3R4R.5R.)-2.5-di(benzyloxy)-3,4dihydroxyhexanediamide.

A Preparation of N1-(2,5-di-O-benzyl)- ,4-mannolactone-6-amide-N6-[tertbutyl valine ester], Tertbutyl valine ester (0.4 g) and the product from Example IB in CH 2 C1 2 (50 ml) was kept at 4 OC for 16 h. Concentration and purification by silica gel column chromatography (EtOAc-hexane 1:2) gave (120 mg).

B Preparation of Nl-[(1S,2R)-2-hydroxy-2,3-dihydro- 1H- I-indenyl]-N6-[tertbutyl valin ester]-(2R,3R,4R,5R,)-2,5-di(benzyloxy)-3,4-dihydroxyhexanediamide. (1S,2R)-1- 49 amino-2-indanol vas added to a solution of the product from above in CH 2

CI

2 and left for 16 h. Concentration and silica gel column chromatography (EtOAc-hexane 1:2) gave (135 mg).

C Preparation of Nl-[(IS,2R)-2-hydroxy-2,3-dihydro- lH-l-indenylj-N6-[vatine acid]- (2R,3R,4R,5R,)-2,5-di(benzyloxy)-3,4-dihydroxyhexanediamide. The product (120 mg) from above was subjected to TFA (5 ml) in 20 min. Concentration and drying in a vacuum gave (96 mg).

D Preparation of the title compound. To the product from above in CH 2 Cl 2 was added 1-hydroxybenzotriazole hydrate (14 gl), 2-aminomethylpyridine (20 mg) and 1,3dicyclohexylcarbodiimide (35 mg). After I h the mixture was directly purified by silica gel column chromatography (EtOAc-MeOH 9:1) gave (38 mg).

EXAMPLE 29

-V

H

,N

O

OH

A

The P1 and P1' filling groups of the para-bromo analogue of the compound of Example 7 were extended as depicted above. Styrene (12.1 mg, 0.1165 mmol) and a little THF were added to a dessicated vessel under nitrogen on an ice bath. 9-BBN was added via injection under agitation. The mixture was allowed to come to room temperature and stirred for 4 hours. The brominated intermediate, prepared analogously to example 7, (30 mg, 0.002 mmol), K 2 CO3 (21.45 mg, 0.155 mmol) and Pd(PPh3) (2.70, 0.002 mmol) in 1 ml DMF were added under nitrogen and heated overnight at 50°C. Around 50 ml chloroform is added and the mixture shaken with 3 x 30 ml brine, dried and evaporated under vacuum overnight. The above depicted product is purified by silica gel chromatography.

NMR (CDC13) 172.5, 170.2, 142.4, 141.4, 133.6, 128.9, 128.3, 128.1, 126.0, 82.0, 73.6, 73.3, 58.3, 37.5, 28.7, 25.9, 19.7, 16.8.

EXAMPLE

A

Tributyl-2-thiazolyl tin (72.6 mg, 0.19 mmol), Pd(PPh3) 4 (2.25 mg, 0.002 mmol), the brominated analogue of Example 7 (30 mg, 0.034 mmol) Ag 2 0 (9.0 mg, 0.04 mmol) in 1 mi DMF were added to a microtube and microwaved for 2 minutes at 60 W. 50 ml chloroform was added and the mixture washed with 3 x 20 ml brine. The organic phase was dried and evaporated ot yield the title compound.

"C NMR (CDCl 3 8 173.3, 172.7, 144.0, 140.4, 133.8, 129.4, 127.5, 120.4, 81.2, 72.8, 72.2, 59.3, 31.1, 26.5, 19.9, 18.1.

EXAMPLE 31 0 S0 OH 0 0 0 O OH 0

-"O

A mixture of the brominated analogue of Example 7, (38.6 mg, 0.05 mmol), methyl acrylate (21.5 mg, 0.25 mmol), diisopropyl ethylamine (25.8 mg, 0.2 mmol), Pd(OAc) 2 (1.12 mg, 0.05 mmol), (o-tol) 3 P (3.65 mg, 0.012 mmol) and water (0.15 ml) in DMF (0.85 ml) was degassed under a nitrogen flow for 10 minutes. The reaction mixture was stirred and heated to 100°C for 48 hours. The reaction mixture was allowed to cool and poured into saturated acqueous sodium chloride solution (10 ml). The aqueous layer was extracted with dichloromethane (3 x 10 ml) and the combined organic phases were washed with saturated aqueous sodium chloride solution (3 x 5 ml), dried over MgSO4, filtered and concentrated at reduced pressure. The residue was purified on a silica gel column to give the above depicted product (27 mg, 69%).

52 "C NMR (CDC1 3 5 171.7, 171.6, 171.5, 167.7, 14.4, 139.2, 134.3, 131.7, 129.6, 128.4, 118.0, 80.5, 77.7, 77.2, 76.7, 72.4, 71.8, 51.8, 49.7, 49.4, 49.1, 48.8, 48.5, 29.8, 25.9, 19.4, 17.3.

EXAMPLE 32 0 OH 0

NN

°0/ Repeating the reaction conditions of example 31 with 2-ethoxy-3-keto-1-cyclohexadiene, gave a mixture of compounds which separated by 7% MeOH in dichloromethane (preparative plate) to yield 66 mg of the crude product depicted above.

"C NMR (CDC13) 5 171.5, 137.3, 137.0, 128.5, 128.2, 128.0, 127.4, 80.5, 77.5, 77.2, 77.0, 76.5, 72.4, 71.8, 67.9, 58.0, 50.0,49.7, 49.3, 49.0, 48.7, 48.4, 48.1, 38.7, 30.6, 29.5, 25.6, 22.3, 19.3, 17.1, 15.1 EXAMPLE 33

N

0 0

,N

mA N II~ H.

To a solution of the compound of example 32 (18 mg, 0.02 mmol) in CH 2 C1 1 was added I- N-boron tribromide 1 ml) dropwise over 5 minutes at -78 The reaction mixture was stirred at this temperature for 30 min and the n kept in a refrigerator for 2 hours at -12'C. the reaction mixture was poured into sat. aqueous NaCI solution (5 ml), extracted with dichloromethane (3 x 5 ml) and the combine organic layers washed with sat. aqueous NaCI solution (2 x 5 ml), dried over MgSO 4 filtered and concentrated at reduced pressure. The residue was purified on a silica gel column to give a crude preparation of the above depicted structure, (9.8 mg, 58%).

3 C NMR (CDCI 3 6 171.5, 136.5, 128.4, 127.9, 127.6, 80.3, 77.5, 76.5, 72.4, 71.8, 58.0, 49.9, 49.6, 49.3, 48.6, 48.3, 48.0, 35.7, 29.5, 28.6, 25.7, 22.4, 19.3, 17.1 Biological Example 1 Representative compounds were tested for HIV protease activity in a spectrophotometric enzyme assay using the chromogenic substrate His-Lys-Ala-Arg-Val-Leu-p-nitro-Phe-Glu- Ala-Nle-Ser-amide and purified HIV proteinase. The rate of cleavage is followed by continuously registering the change in absorbance at 300 nm. The IC 5 0 representing the compound concentration which inhibits enzyme performance by 50% is calculated from the dose response curve. However, the compounds of the invention are so extremely active that affinity constants (Ki) are idicated to provide a more effective comparison between compounds.

Compounds are also tested for HIV protease activity in cell culture. MT4 cells grown in RPMI 1640 cell culture medium including 10 fetal calf serum are infected with 10 TCID HIV-1 per 2 x 10 5 cells and cultured for 6 days. XTT is added and the amount of XTT formazan produced in the following 6 hours represents the number of surviving cells.

Results are expressed as the ED 5 0 that is the concentration in gg/ml of the compound of the invention which suppresses viral replication by Antiviral activities for representative compounds of the invention are shown in Table 1.

TABLE 1 ICso .M (Ki M) ED5o tg/ml Example 1 0.015 Example 3 0.004 Example 4 (0.009) 1 Example 7 (0.0023) Example 8 (0.0034) 6 Example 9 (0.0035) 0.4 Example 10 (0.0016) 0.05 4* TABLE 1 Cont. (Ki pM) ECjo Example 11 0.0012 0.2 Example 12 (0.00013) 0.04 Example 13 (0.00007) 0.2 Example 14 (0.00067) 0.04 Example 15 (0.0012) 0.03 Example 16 (0.0023) 1 Example 17 (0.002) 0.8 Example 18 (0.050) Example 19 (0.0005) 0.8 Example 20 (0.0001) 0.9 Example 21 (0.00038) 0.1 Example 22 (0.00061) 0.4 Example 23 (0.0019) 2 Example 24 (0.0014) 0.4 Example 25 (0.00017) 0.02 Example 26 (0.00097) 0.8 Example 27 (0.0014) 0.8 Example 28 ND 0.2 Example 31 (0.00009) 0.3 Example 32 (0.0003) 0.2 Example 33 (0.0005) 0.2 20-07-'06 14:26 FROM- T-595 P011/019 F-438 I:"O1ftY.IAIUo6Un75SS3 Lqpd-OtO% Va 8 -56ci

S

0 The reference in this specification to any prior publication (or information derived from it), or to any matter which is known, is not, and should not be taken as an acknowledgment or admission or any form of suggestion that that prior publication (or information derived from it) or known matter forms part of the common general knowledge in the field of endeavour to which this specification relates.

oThroughout this specification and the claims which follow, unless the context requires cotherwise, the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.

COMS ID No: SBMI-04222139 Received by IP Australia: Time 14:36 Date 2006-07-20

Claims (2)

1. 20-07-06 14-26 FROM- T-595 P012/019 F-438 P1OPERWMALU006CS75IS3 lp.4v-*0"7h
2. 57- THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS: 1. A compound of the formula: wherein; Z' and Z" are independently benzyl unsubstituted or substituted with one to three substituents selected from halo, methoxy, hydroxy, amino, cyano, nitro, hydroxymethyl, aminomethyl, morpholinethoxy, alkylsulfonyl, carbamoyl, benzyloxy, phenyl (itself substituted as defined herein) or a 5 or 6 membered heterocycle containing one or two hetero atoms such as thiophene, thiazole, pyrimidine, pyridine, N-morpholine, N- piperidine, N-piperazine, N'-methyl-N-piperazine, N-pyrrolidone or N-pyrrolidine, optionally substituted as defined herein; and pharmaceutically acceptable salts thereof 2. A compound according to claim 1, wherein Z' and/or Z" is benzyl, 2-fluorobenzyl, 2-methylbcnzyl, 2,4-difluorobenzyl, 4-fluorobenzyl, 4-bromobenzyl, 4-phenylbenzyl, 4- thiophenylbenzyl, 4-(4'-nitrophenyl)benzyl, 4-thienylbcnzyl, 4-thiazolylbenzyl or 4- (pyridyl)benzyl. 3. A compound according to claim I or claim 2 wherein Z' and Z" are the same. 4. A compound according to any one of claims 1 to 3, having the 2R, 3R, 4R, configuration. COMS ID No: SBMI-04222139 Received by IP Australia: Time 14:36 Date 2006-07-20 20-07-'06 14:26 FROM- T-595 P013/019 F-438 PrIPERWA*L12W0S libI 7 fl #tap A O 0 58- 5. The compound N1,N6-di[(l S,2R)-2-hydroxy-2,3-dihydro-l H-l-indenyl]- o (2R,3R,4R,5R)-2,5-di(2-fluorobenzyloxy)-3,4-dihydroxyhexanediamide or a pharmaceutically acceptable salt thereof, 6. A pharmaceutical composition comprising a compound according to any one of claims 1 to 5 and a pharmaceutically acceptable carrier or diluent therefor. Ci o 7. A pharmaceutical composition according to claim 6, further comprising one to 0 C' three additional HIV antivirals selected from AZT, ddl, ddC, D4T, ritonavir, saquinavir, indinavir, nelfinavir, delavirdine, nevirapine, PFA and H2G. 8. Use of a compound according to any one of claims 1 to 5 in the manufacture of a medicament for the treatment of HIV. 9. A method for inhibiting the replication of HIV comprising administering an effective amount of a compound according to any one of claims 1 to 5 to a subject afflicted with HIV. A compound according to any one of claims 1 to 4 substantially as hereinbefore described. DATED this 2 0 t h day of July, 2006 Medivir AB By DAVIES COLLISON CAVE Patent Attorneys for the Applicant COMS ID No: SBMI-04222139 Received by IP Australia: Time 14:36 Date 2006-07-20