AU2002300275B2 - Composition for controlling harmful bio-organisms and method for controlling harmful bio-organisms using the same - Google Patents

Description

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AUSTRALIA

Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT Applicant: ISHIHARA SANGYO KAISHA, LTD.

Invention Title: COMPOSITION FOR CONTROLLING HARMFUL BIO-ORGANISMS AND METHOD FOR CONTROLLING HARMFUL BIO-ORGANISMS USING THE SAME The following statement is a full description of this invention, including the best method of performing it known to me/us: .9 T

DESCRIPTION

COMPOSITION FOR CONTROLLING HARMFUL BIO-ORGANISMS AND METHOD FOR CONTROLLING HARMFUL BIO-ORGANISMS USING THE SAME Technical Field This invention relates to a composition having markedly enhanced controlling effects on harmful bioorganisms, especially curative and/or preventive effects on plant diseases, and are useful in agriculture and horticulture; a method for controlling harmful bioorganisms using the composition; and a method for enhancing the harmful bio-organism controlling effects of a harmful bio-organism controlling agent.

Background Art With reference to a combination of active ingredients and used in the present invention (hereinafter described), EP Patent No. 298196 teaches that the imidazole compound used in the present invention as active ingredient is useful as a harmful bio-organism controlling agent, referring to the possibility of using the compound in combination with other fungicides if desired. EP Patent No. 298196 adds that a combined use of an imidazole compound structurally similar to the imidazole compound used in the present invention as active ingredient with other fungicides, such as cyanoacetamide compounds 1-(2-cyano-2-methoxyiminoacetyl)-3-ethylurea) and organic chlorine compounds tetrachloroisophthalonitrile), brings about enhanced effects. Furthermore, EP Patent No. 337103 discloses a harmful bio-organism controlling agent containing at least one imidazole compound structurally similar to the imidazole compound used in the present invention as an active ingredient and at least one active ingredient selected from a cyanoacetamide compound, an organic chlorine compound (including tetrachloroisophthalonitrile), a phenylamide compound (including methyl N- (2-methoxyacetyl)-N-(2, 6-xylyl)-DLalaninate), a cinnamic acid compound, a copper compound, and an organophosphorus compound (including Fosetyl- Aluminum) Reviewing these patents in view of the present invention, they do not describe nor suggest a combination of the imidazole compound as active ingredient and an inorganic phosphorus compound, a p-methoxyacrylate compound or an oxazolidinedione compound. Neither do they describe nor suggest the pronouncedly excellent controlling effects which may be possessed by a composition comprising the imidazole compound as one active ingredient and at least one member selected from the group consisting of a cyanoacetamide compound, an organic chlorine compound, a phenylamide compound, a cinnamic acid compound, a copper compound, and an organophosphorus compound as the other active ingredient.

With respect to a combination of active ingredient and activity-enhancing ingredient (c) (hereinafter described), EP Patent No. 298196 describes usefulness of the imidazole compound of the present invention as a harmful bio-organism controlling agent, teaching that this compound can be formulated into various forms together with adjuvants. JP-A-Heisei-3- 11003 (the term "JP-A" as used herein means an "unexamined published Japanese patent application") discloses a method for controlling harmful bio-organisms comprising applying an aqueous dispersion containing at least one of the imidazole compounds of the present invention and a sorbitan higher fatty acid ester surface active agent.

The imidazole compound represented by formula (I) and many other conventional harmful bio-organism controlling agents have their several characteristics in the controlling effects. Some produce insufficient effects on some harmful bio-organisms, or some are less effective in curing than in prevention, or some have relatively short duration in residual effect. Therefore, cases are sometimes met with in which their controlling effects on harmful bio-organisms are insufficient in practice in some uses. Further, although the imidazole compound of formula exhibits excellent fungicidal effects on Phycomycetes, it tends to fail to produce sufficient curative and/or preventive effects depending on the situation of the development of a disease. From this aspect, too, enhancement has been desired.

On the other hand, in practical application of a harmful bio-organism controlling agent comprising the imidazole compound of formula it is desirable to minimize the amount of the compound to be used for cost saving while trying to control a plurality of harmful bio-organisms different in kind, the time of disease breakout or the time of occurrence as much as possible.

Further, while the harmful bio-organism controlling agent containing the imidazole compound of formula (I) as an active ingredient is particularly excellent in preventive effect, it has been demanded to enhance its curative effect.

Disclosure of the Invention The inventors of the present invention have studied in order to settle the above-mentioned problems and have found as a result that a combined use of the imidazole compound of formula as active ingredient and a specific compound as active ingredient (b) 'produces unexpected results such that the respective amounts of the compounds can be reduced or the respective control spectra are broadened as compared with their individual use. They have also found that a combined use of active ingredient with activityenhancing ingredient brings about marked enhancement in controlling effect, particularly curative effect, as compared with the use of active ingredient alone, thereby making it possible to reduce the amount of active ingredient The present invention has been reached based on these, findings.

In a first aspect, the present invention provides a composition for controlling harmful bio-organisms comprising at least one imidazole compound represented by formula Cl

N

S0 2

N(CH

3 2 wherein R represents a lower alkyl group or a lower alkoxy group; and n represents an integer of 1 to as an active ingredient, and at least one inorganic phosphorus compound and/or at least one fungicide for Phycomycetes which is at least one member selected from the group consisting of a P-methoxyacrylate compound, an oxazolidinedione compound, a phenylamide compound, a cinnamic acid compound and a copper compound as an active ingredient.

In a second aspect, the present invention provides a method for controlling harmful bio-organisms comprising applying a composition for controlling harmful bio-organisms onto harmful bio-organisms, wherein the composition comprises: at least one imidazole compound represented by formula Cl

NC

S0 2

N(CH

3 2 wherein R represents a lower alkyl group or a lower alkoxy group; and n represents an integer of 1 to as an active ingredient, and at least one inorganic phosphorus compound and/or at least one fungicide for Phycomycetes which is at least one member selected from the group consisting of a -methoxyacrylate compound, an oxazolidinedione compound, a phenylamide compound, a cinnamic acid compound and a copper compound as an active ingredient.

Also described herein is a composition for controlling harmful bio-organisms comprising at least one imidazole compound represented by formula NI R)

N(CH

3 2 wherein R represents a lower alkyl group or a lower alkoxy group; and n represents an integer of 1 to as an active ingredient, and at least one inorganic phosphorus compound and/or at least one fungicide for Phycomycetes as an active ingredient or a spreader as an activity-enhancing ingredient.

Best Mode for Practicing Invention In formula the lower alkyl group or the alkyl moiety of the lower alkoxy group as represented by R includes an alkyl group having 1 to 6 carbon atoms, such as methyl, ethyl, propyl, butyl, pentyl or hexyl, which may have either a straight chain or a branched chain. When n is 2 or greater, the plural Rs may be the same or different.

The imidazole compounds represented by formula include the following compounds: 4-chloro-2-cyano-l-dimethylsulfamoyl-5-(4methylphenyl)imidazole (Compound No. 1); 4-chloro-2-cyano-1-dimethylsulfamoyl-5- (4methoxyphenyl)imidazole (Compound No. 2); 4-chloro-2-cyano-l-dimethylsulfamoyl-5-(4ethylphenyl)imidazole (Compound No. and 4-chloro-2-cyano-l-dimethylsulfamoyl-5- (3-methyl- 4-methoxyphenyl)imidazole (Compound No. 4).

The imidazole compounds of formula can be prepared by known processes disclosed, in EP Patent No. 298196 or EP-A-705823.

The inorganic phosphorus compounds as active ingredient include phosphoric acid, phosphorous acid, hypophosphorous acid, condensed phosphoric acid, condensed phosphorous acid, and salts thereof. The salts include those with light metals (specific gravity: less than 4) such as alkali metals, alkaline-earth meals, aluminum, etc.; heavy metals (specific gravity: 4 or more), such as zinc, copper, nickel, manganese, etc.; and substituted or unsubstituted ammonium salts.

Salts of phosphoric acid include primary phosphates sodium dihydrogenphosphate, potassium dihydogenphosphate, aluminum dihydrogenphosphate, ammonium dihydrogenphosphate, calcium dihydrogenphosphate), secondary phosphates disodium hydrogenphosphate, dipotassium hydrogenphosphate, diammonium hydrogenphosphate, dimagnesium hydrogenphosphate), and tertiary phosphates trisodium phosphate, tripotassium phosphate, zinc phosphate, aluminum phosphate, ammonium phosphate, ammonium magnesium phosphate, magnesium phosphate, calcium phosphate).

Salts of phosphorous acid include primary or secondary phosphites sodium primary or secondary phosphite, potassium primary or secondary phosphite, calcium primary or secondary phosphite).

Salts of hypophosphorous acid include sodium hypophosphite, barium hypophosphite, and calcium hypophosphite.

Condensed phosphoric acids and salts thereof include polyphosphoric acids pyrophosphoric acid), and polyphosphates sodium pyrophosphate, calcium pyrophosphate, disodium dihydrogenpyrophosphate).

Condensed phosphorous acids and salts thereof include polymetaphosphoric acids trimetaphosphoric acid, tetrametaphosphoric acid) and polymetaphosphates sodium trimetaphosphate, sodium tetrametaphosphate, sodium hexametaphosphate).

The fungicides for Phycomycetes which can be used as active ingredient include: p-methoxyacrylate compounds methyl cyanophenoxy) pyrimidin-4-yloxy] phenyl} -3-methoxyacrylate, methyl (a)-methoxyi'milno [c-(o-tolyloxy) -0tolyll acetate); oxazolld-inedione compounds 3 -anilino-5-methyl-5. (4-phenoxyphenyl) 1 oxazolidine-2,/4-dione); phenylamide compounds (e methyl N- (2-methoxyacetyl) (2,6-xylyl) ,DL-alan-inate (common namne Metalax~yl) 2-methoxy-N- (2-oxo-l 3 -oxazolidin-3-yl) aceto- 2' ,6'-xylidide (common name: Oxadixyl), ()-ca-2-chloro-N- (2 ,E-xylylaceta=nide) -ybutyrolactone (coirmon name: Ofurace), methyl N-phenylacetyl-N- E-xylyl) -DL-alaninate (common name: Benalaxyl), methyl N- (2-furoyl) 6--xylyl) -DL-alaninate (commIron name E'iuralaxyrl), 3 -ch-lor-ophenyl) cyclopropanecarboxamide] -y-Ibutyrolactone (common name: Cyprofuram)); c-innamic acid compounds (E,Z)-4-[3-(4-chlorophenyl)-3(34.

diinethoxyphenyl) acryloyllmo-rpholine (common name: Diinethomorph)); and copper compounds (e~.,organi-c or inorganic copper fungicides).

Of these, 2 -cyano-Dhenoxy)pyrirnidin- 4 -yloxylphenyl)I- 3 -methoxyacrylate (hereinafter referred to as "compound methyl (E)-methoxyimino (otolyloxy)-O-tolyl]acetate' (hereinafter referred to as "compound and 3-anilino-5-methyl-5-(4phenoxyphenyl) -l,-3-oxazolidine-2.,4-dione (hereinafter referred to as "compound are described in Brighton Crop Prot. Conf. Pests and Diseases, pp. 435-443 (1992), ibid, pp. 403-410 (1992) and ibid, pp. 21-26 (1996) respectively.

Of the phenylamide compounds, methyl N-(2methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate is preferred. Of the copper compounds, an inorganic copper fungicide is preferred.

The inorganic or organic copper fungicides as referred to the above include fungicidal preparations containing chemicals (such as fungicides, etc.) other than active ingredients and in addition to the copper compound.

The inorganic copper fungicides include those containing copper oxysulfate as an active ingredient, such as Sanpun Bordeaux (trade name, produced by Daiichi Noyaku K.K. and Eokko Chemical Industry Co., Ltd.) and Sanpun Bordeaux Dust DL (trade name, produced by Dai-ichi Noyaku K.K. and Hokko Chemical Industry Co., Ltd.); those containing copper oxychloride as an active ingredient, such as San Bordeaux (trade name, produced by Sankei Chemical Co., Ltd.) Doitsu Borudo A (trade name, produced by Dai-ichi Noyaku K.K. and Bokko Chemical Industry Co., Ltd.), Do-cal Wettable Powder (trade name, produced by Yashima Chemical Industry Co., Ltd.), Do-jet (trade name, produced by Nissan Chemical Industries, Ltd.), etc.; those containing cupric hydroxide as an active ingredient, such as Kocide Bordeaux, Kocide DF, Kocide SD (trade names, all produced by Griffin), etc.; and those containing anhydrous copper (II) sulfate, such as Gandie Wettable Powder (trade name, produced by Agro-Kanesho Co., Ltd.), etc.

The fungicidal preparations containing the inorganic copper fungicide and chemicals (such as fungicides, etc.) other than ingredients and (b) include a Bordeaux mixture containing basic copper calcium sulfate; copper-sulfur fungicides, such as Engei Bordeaux (trade name, produced by Sankei Chemical Co., Ltd.), etc.; copper-validamycin fungicides; coppervalidamycin-fthalide fungicides; copper-pyrifenox fungicides; copper (I)-vinclozolin fungicides; copperfthalide fungicides; copper-procymidone fungicides, such as Scletane Wettable Powder (trade name, produced by Hokko Chemical Industry Co., Ltd.) copper (I)-fosetyl wettable powders; copper-metalaxyl fungicides, such as Ridomil Copper Wettable Powder (trade name, produced by Nihon Nohyaku Co., Ltd.); iprodione copper

(I)

fungicides, such as Daisedo Wettable Powder (trade name, produced by Yashima Chemical Industry Co., Ltd.); iminoctadine triacetate-copper fungicides; oxadixyl copper fungicides; oxolinic acid-copper fungicides; kasugamycin-copper fungicides, such as Kasumin Bordeaux Dust 3DL (trade name, produced by Hokko Chemical Industry Co., Ltd.), Kasumin Bordeaux (trade name, produced by Dai-ichi Noyaku K.K. and Hokko Chemical Industry Co., Ltd.), etc.; dithianon copper (I) fungicides; streptomycin-copper fungicides, such as Do- Stomy Wettable Powder (trade name, produced by Nihon Nohyaku Co., Ltd.), etc.; sodium hydrogencarbonatecopper fungicides, such as G-Fine Wettable Powder (trade name, produced by Yashima Chemical Industry Co., Ltd.); and copper-organocopper fungicides, such as Oxy Bordeaux (trade name, produced by Sankyo Co., Ltd.), Kinset Wettable Powder (trade name, produced by Agro-Kanesho Co., Ltd.), Kinset Wettable Powder 80 (trade name, produced by Agro-Kanesho Co., Ltd.), etc.

Of these inorganic copper fungicides, it is particularly preferred to use those containing one or more active ingredients selected from the group consisting of cupric hydroxide, copper oxysulfate, copper oxychloride, anhydrous copper (II) sulfate, and basic copper calcium sulfate.

The organic .copper fungicides include 8-hydroxyquinoline copper fungicides, such as Quinone-do Wettable Powder 40 or 80 (trade name, produced by Agro- Kanesho Co., Ltd.), Quinone-do Granules (trade name, produced by Agro-Kanesho Co., Ltd.), Quinone-do Flowable (trade name, produced by Agro-Kanesho Co., Ltd.), Oxinecopper Wettable Powder (trade name, produced by Tomono Agrica Co., Ltd.), Oxine-copper Wettable Powder 75 (trade name, produced by Tomono Agrica Co., Ltd.), Oxine-copper Wettable Powder 80 (trade name, produced by Tomono Agrica Co., Ltd. and Nissan Chemical Industries, Ltd.), Oxine-copper Flowable (trade name, produced by Tomono Agrica Co., Ltd. and Nissan Chemical Industries, Ltd.), Dokirin Wettable Powder 80 (trade name, produced by Nihon Nohyaku Co., Ltd.), and Dokirin Flowable (trade name, produced. by Nihon Nohyaku Co., Ltd.), etc.; copper hydroxynonylbenzenesulfonate fungicides such as Yonepon (trade name, produced by Yonezawa Kagaku etc.; copper (II) bis (ethylenediamine)bis (dodecylbenzenesulfonate) fungicides, such as Sanyol (trade name, produced by Otsuka Chemical Co., Ltd. and Yonezawa Kagaku etc.; and copper terephthalate fungicides.

The fungicidal preparations containing the organic copper fungicide and fungicides other than ingredients and include iprodione organocopper fungicides, oxolinic acid-organocopper fungicides, captan -thiuram-organocopper fungicides, captan (I)-organocopper fungicides, dithianon organocopper fungicides, streptomycin-organocopper fungicides, thiabendazole (I)-organocopper fungicides, fenarimol (I)-organocopper fungicides, machine oilorganocopper fungicides, and guazatine iminoctadineorganocopper fungicides.

Also described herein are spreaders. A spreader is used as activity-enhancing ingredient Examples of spreaders include surface active agents (exclusive of sorbitan higher fatty acid esters), paraffin oil', animal and/or vegetable oil, and mineral oil. In general, spreaders are not definitely classified. Some of animal and/or vegetable oil, and mineral oil serve as surface active agents, and there are some spreaders called stickers that cannot be classified clearly. Any spreader that appreciably enhances the physical properties of the imidazole compound of formula such as fixing properties, penetrability, spreadability, and stomatal flooding properties, to enhance the effects of the compound can be used. Typically, the physical properties of the imidazole compound of formula could be enhanced by the spreader to bring about such an effect that equal harmful bio-organism controlling effects are obtained with a lesser amount of the compound. Of the above-described spreaders preferred are surface active agents (exclusive of sorbitan higher fatty acid esters), animal and/or vegetable oil, and mineral oil. Still preferred are nonionic surface active agents (exclusive of sorbitan higher fatty acid esters), animal and/or vegetable oil, and mineral oil.

Suitable nonionic surface active agents which can be used as activity-enhancing ingredient include polyoxyethylene alkyl ethers, polyoxyethylene alkylphenyl ethers; polyoxyethylene aryl ethers, polyoxyethylene glycol alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene polyol fatty acid esters, polyoxyethylene fatty acid amides, amine Noxides such as Aromox C/12W (trade name, produced by Akzo Chemie), polyoxyethylene alkylamines, glycerol fatty acid esters, silicone surface active agents, polyoxyethylene alkyl thioether polyphosphate surface active agents such as Reider (trade name, produced by American Trading Company), higher alcohol sulfuric acid esters, and dialkylsulfosuccinates. Among these, preferred are polyoxyethylene alkyl ethers, polyoxyethylene alkylphenyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene fatty acid amides, silicone surface active agents, higher alcohol sulfuric acid esters, and dialkylsulfosuccinates. Still preferred are silicone surface active agents, polyoxyethylene alkylphenyl ethers and polyoxyethylene fatty acid esters. Silicone surface active agents, especially DyneAmic (trade mark, produced by Setre Chemical) and KINETIC (trade mark, produced by Setre Chemical), and SILWETT L-77 (produced by Witco),and SLIPPA (produced by Interagro) are particularly preferred.

Specific examples of preferred nonionic surface active agents are listed in Table 1 below. Additionally polyoxyethylene polysilane ether (a kind of silicone surface active agents), Renex 36 (trade name, polyoxyethylene alkyl ether produced by Bayer AG), Crop Oil Extra (trade name of a polyoxyethylene alkylphenyl ether produced by Kalo, Inc.), Ortho X-77 Spreader (trade name, produced by Chevron Chemical Company) and COOP Spreader Activator (trade name, produced by Formland Industry) are also included in usable nonionic surface active agents.

Tabae 1 Nonionic Surface Active Agents Trade Name No. Kind Designation (Ma (Manufacturer) S Polyoxyethylene Genapol LRO Fluid alkyl ether (Hoechst AG) ethylene oxide adduct Lutensol TO 7 (BASF of iso-C 13 oxo alcohol AG) Cio oxo alcohol ethylene oxide adduct Lutensol ON 60 (BASF 3 ethylene of Cio oxo alcohol AG) oxide Polyoxyethylene AGRAL 30 (ICI 4 alkylphenyl Agrochemicals) ether 90% nonyl phenoxy AGRAL 90 (ICI polyethoxyethanol Agrochemicals) 6nonirfenpl AGRAL PLUS (ICI polietilenglicol eter Agrochemicals) ARKOPAL N-100 7 (Hoechst AG) ethylene oxide Citowett (BASF AG) or condensate CITOWETT 9" Genapol X-60 (Hoechst

AG)

ethoxylated fatty Frigate® (ISK amine Biotech Europe, Ltd.) polyoxyethylene polyoxyethylene SOPROPHOR® BSU 11 aryl ether tristyrylphenyl ether

(RHONE-POULENC)

polyoxyethylene polyoxyethylene KUSARINO (Nihon 12 alkylphenyl octylphenyl ether Nohyaku Co., Ltd.) ether Noigen EA110 (Dai- 13 ichi Kogyo Seiyaku Co., Ltd.) polyoxyethylene polyethylene glycol TOKUSEI RINO (Nihon 14 alkylphenyl alkylphenol ether Nohyaku Co., Ltd.) ether lignin lignin sulfonate sulfonate (12%) Table 1 (cont'd.) Trade Name No. Kind Designation (Manufacturer) polyoxyethylene polyoxyethylene RHODASURF0 860/P fatty acid aliphatic alcohol ester

(RHONE-POULENC)

16 polyoxyethylene D-3605 (Takemoto Oils soybean amino ether and Fats Co., Ltd.) polyoxyethylene D-230 N (Takemoto 17 castor oil ether Oils and Fats Co., Ltd.) polyoxyethylene rape D-233 N (Takemoto 18 seed oil ether Oils and Fats Co., Ltd.) polyoxyethylene oleyl Noigen ET-120E (Dai- 19 ether ichi Kogyo Seiyaku Co., Ltd.) "polyoxyethylene fatty Spray Sticker (Nihon acid ester Nohyaku Co., Ltd.) silicone proprietary blend of DyneAmic (Setre surface active polyalkylene oxide- Chemical) agent modified 21 polymethylsiloxane, nonionic emulsifiers, and methylated vegetable oils proprietary blend of KINETIC (Setre polyalkylene oxide- Chemical) 22 modified polydimethylsiloxane and nonionic surface active agents (No.23) silicone SILWETT L-77 (Witco) 23 polyalkylene oxide modified polydimethyl siloxane Silicone silicone polyalkylene SLIPPA (Interagro) surface active oxide modified Organosilicone agent (No.23 polydimethyl siloxane (Silwet L-77) 24 and Linear and linear alcohol alcohol surfactant blend surfactant blend) polyoxyethylene ethylene oxide-adduct Lutensol FSA10 (BASF fatty acid of fatty acid amide AG) amide Table 1 (cont'dfl No. Designation Trade Name (Manufacturer) 26 octylphenoxy Citowett PLUS (BASF AG) or polyethoxyethanol CITOWETT PLUS 27 dilauryl ester polyethylene COADJUVANT Chevron (Bayer AG) glycol ester solvent c.s.p.

28 polioxiester amino grass 80g, Hi-Point (CARGIL) solvente 29 polyoxyethylene rosin ester Sorpol 7261 (Toho Chemical Industry Co., Ltd.) diglycerin diol fatty acid Sorpol 7337 (Toho Chemical ester polyoxyethylene Industry Co., Ltd.) monomethyl ether 31 polyoxyethylene rosin ester Sorpol 7445 (Toho Chemical Industry Co., Ltd.) 32 trimethylnonyl Surfactant WK polyethoxyethanol 33 polyglycol alkylaryl ether TREND@ du Pont) 34 ethylene-acrylic acid Poligen WE3 (BASF AG) copolymer emulsion Pepol AH-053 Lot. No. 2184Y (Toho Chemical Industry Co., Ltd.) 36 benceno Surfonato de sodio COADJUVANT TRITON ACT-M eter 37 Acetite Mineral 85% COADJUVANT ACETITE ANPLUS 38 Acetite Mineral 83% COADJUVANT ASSIST OIL 39 linear alcohol ethoxylate 7 Ethylan D257 mols EO Suitable anionic surface active agents which can be used as activity-enhancing ingredient include sulfuric ester surface active agents, such as alkylsulfuric esters or salts thereof; sulfonic acid surface active agents, such as naphthylmethanesulfonates and lignin sulfonates; fatty acid salts; and fluorinecontaining surface active agents, with sulfuric ester and sulfonic acid surface active agents being preferred.

Examples of preferred anionic surface active agents are shown in Table 2 below.

t Table 2 Anionic Surface Active Aaents Trade Name No. Kind Designation (Manufacturer) higher alcohol sodium higher alcohol Monogen Y-100® (Daisulfuric acid sulfate ichi Kogyo Seiyaku ester Co., Ltd.

dialkylsulfo- sodium New Kalgen 41 succinate dialkylsulfosuccinate (Takemoto Oils Fats Co., Ltd.) Ssodium di-2- Genopur SB 1970J 42 ethylhexyl- (Hoechst AG) sulfosuccinate 43 sodium Hostapon.T Pow. H/C oleylmethyltauride (Hoechst AG) alkyl diglycol ether Genapol LRO paste sulfate salt based on (Hoechst AG) 44 natural fatty alcohols; RO- (EO) 2-SOJNa linosulfa to de COADJUVANT RINO calcio 20+12 Suitable cationic surface active agents which can be used as activity-enhancing ingredient include dialkylammonium salts such as NEEDS (a trade mark, produced by Kao Corporation) and alkylammonium salts such as Arguard T/50 (trade name, produced by Akzo Chemical) as shown in Table 3 below.

C ationic Surface Active ents Cationic Surface Active Agents Trade Name No. Kind Designation (Manufacturer) dialkylammonium polynaphthyl- NEEDS (Kao salt methanesulfonate Corporation) 46 dialkyl dimethylammonium polyoxyethylene fatty acid ester polyoxyethylene RHODAMEEN® 47 aliphatic amine

(RHONE-POULENC)

The animal and/or vegetable oil which can be used as activity-enhancing ingredient include vegetable oil, such as corn oil, soybean oil, linseed oil, sunflower oil, cotton seed oil, and rape seed oil; and animal oil, such as beef tallow and train oil (whale oil) Alkylated g. methylated) vegetable oil such as SCOIL (manufactured by MVRC) is also included.

Specific examples of suitable animal and/or vegetable oil are shown in Table 4 below. Among these, alkylated vegetable oil is preferred.

Table 4 Animal and/or Vegetable Oil No. Kind No. Kind (Manufacturer) 48 corn oil 53 soybean 90% emulsion 49 corn oil emulsion 54 soybean oil corn oil emulsion 55 SCOIL (MVRC) corn oil emulsion linseed oil emulsion 51 56 (ICI Agrochemicals) soybean oil animal oil Ethokem 52 57 (Midkem Agrochemicals) No. Kind Designation Trade Name coconut modified coco Seawet or SEA Soil diethanolamide/inert WET (Sea-Born 58 ingredients couplers Subsidiary and solubilizing agents Lane, Inc.) The paraffin oil which can be used as activityenhancing ingredient includes product originated from animal and/or vegetable oil, product originated from mineral oil petroleum), and mixtures thereof. Specific examples are shown in Table 5 below.

Table Paraffin Oil Trade name No. Kind Designation (Manufacturer) (Manufacturer) paraffin ATPLUS 411F (ICI oil Agrochemicals) ATPLUS 411F OIL (ICI Agrochemicals) nonionic SUN OIL ADJUVANT 61 surfactant (Schering Agrochemicals, Ltd.) petroleum based OLEOTAN (Biomex) 62 paraffinic oil nonionic surfactants 63 PRIME OIL The mineral oil which can be used as activityenhancing ingredient include machine oil, fuel oil, and silicone oil. Examples of preferred mineral oil are shown in Table 6 below. Among these, ISHIOIL (manufactured by Ishihara Sangyo Kaisha, Ltd.) is the most preferred.

Mineral Oil Trade Name No. Kind Designation (anacrer (Manufacturer) mineral machine oil oil emulsion 64 (machine oil) machine oil 65 emulsion (Toho Chemical Industry Co., Ltd.) mineral diesel engine oil 66 oil (fuel emulsion oil) mineral mineral oil ISHIOIL (Ishihara 67 oil tensio activo Sangyo Kaisha, Ltd.) blend of heavy AGRI-DEX or Agrirange paraffin Dex(Helena base petroleum Chemical Company) 68 oil, polyol fatty acid esters, and polyethoxylated derivatives The above-described spreaders, surface active agents (except sorbitan higher fatty acid esters), animal and/or vegetable oil, paraffin oil, mineral oil, etc. can be combined appropriately for use as activityenhancing ingredient Combinations of two or more spreaders include vegetable oil containing surface active agents, such as Soy Dex (Helena Chemical Company), etc.; and paraffin oil containing surface active agents, such as Oleo DP 11E du Pont), Fyzol 11E (Schering Agrochemicals) Agri Dex (Helena Chemical Atplas 411 (ICI Agrochemicals), Herbimax (Love Land Industries, Inc.), Competitor Crop Oil Concentrate (Red Pancer Chemical) Actipron (Oil DASH (BASF AG) Atlas Adherb (Atlas Interlates, Ltd.), Cropspray (Tribart Farm Chemical) Agravia -11E (Wakker Chemie), Penetrator (Helena Chemical Atlus Adjuvant Oil (Atlus Interlates, Ltd.), etc. Mixed spreaders shown in Table 7 are also included.

Table 7 Mixed Spreaders No. Designation Trade Name mineral oil vegetable mineral oil emulsion 69 oil soybean oil emulsion 2-pyrrolidione Agrimax 3H 1-octyl-2-pyrrolidione 1-ethenyl-l-hexadecyl homopolymer calcium dodecylbenzenesulfonate As stated above, any spreader can be used as far as it significantly enhances the physical properties of the imidazole compound of formula to enhance the effects of the compound, whatever kind it belongs to. Specific examples of useful spreaders that cannot be classified into any of the above listed kinds are shown in Table 8 below. As a matter course, a composition for controlling harmful bio-organisms containing at least one imidazole compound of formula as active ingredient having incorporated therein the spreader usable as activityenhancing ingredient in a ratio according to the present invention is expected to exhibit similar effects.

Unclass i fi -h I ;pr la r -z Unclassi fiahie Snrcn Ac. r cz No. Designation Trade Name (Manufacturer) 71 Adherex MR (ISK Mexico) 72 Atlox-BI (Kao Corporation) sol de olamina del acido EXTRAVON® 40 (Ciba-Geigy dodecil benceno Agrochemicals, Ltd.) Ssulfuronico solvente (aqua) estabilizante e impurezas (67%) 74 SUPER CORAL (Grupocoret) SURFATE 30 du Pont) 76 ALBOL INEUM AK (ICI Agrochemicals) 77 ATPLUS SL 92 (ICI Agrochemicals) Nisseki Noyaku Oil 78 Emulsion (Nippon Oil Co., Ltd.) 79 OLEO RUSTICA 11E (Hoechst

AG)

SURF OIL (Hoechst AG) ADJUVANT No. 1 (Toho 81 Chemical Industry Co., Ltd.) 82 Acetite parafinico 81% Ulvapron® 83 X2-5309 (Toray Industries, Inc.) blend of alkylphenyl Helena Surfix (Helena hydroxy-polyoxyethylene Chemical Co.) 84 polymerized resins and fatty acids paraffin base petroleum oil (22%) Table 8 (cont'd.) No. Designation Trade Name (Manufacturer) Helena Suraid (Helena Chemical Co.) 86 COADJUVANT NATURL OIL (Stoller Chemical Co.) Spolioxy ester amino graso COADJUVANT SP-SUPER 42 synthetic latex Bond (Loveland primary aliphatic Industries, Inc.) 88 oxyalkylated alcohol inert ingredients higher alkyl pyrrolidones Banka (formerly ANKA) combined with water- (Interagro) 89 insoluble polymers (pseudo cationic polymers) and surfactants alkoxylated amine ARMA (Interagro) (alkoxylated fatty amine polymer)/polysaccharide (sugar-based nonionic surfactant and buffer) blend modified LATRON B-1956 (Rohm 91 phthalic/glycerol alkyl Haas Co.) resin butyl alcohol (23%) blend of DL-1-p-menthene LASTIC (Helena Chemical 92 inert ingredients Co.) The above-described fungicides for Phycomycetes as active ingredient characteristically have one or more of a preventive effect, a curative effect and penetrability. Some of the inorganic phosphorus compounds as active ingredient which are not fungicides, possess one or more of a preventive effect, a curative effect, and penetrability similarly to the fungicides for Phycomycetes.

The term "fungicides having a preventive effect" means that the fungicides have an ability of preventing plant diseases. Examples of such fungicides include pmethoxyacrylate compounds, oxazolidinedione compounds, cyanoacetamide compounds, organic chlorine compounds, phenylamide compounds, cinnamic acid compounds, copper compounds, and organophosphorus compounds.

The term "fungicides having a curative effect" means that the fungicides can migrate through the plant body to control an invading harmful bio-organism.

Examples of such fungicides include p-methoxyacrylate compounds, cyanoacetamide compounds, phenylamide compounds, cinnamic acid compounds, and organophosphorus compounds.

The term "fungicides having penetrability" means that the fungicides having an ability of penetrating through the surface of leaves into the inside. Examples of such fungicides include p-methoxyacrylate compounds, oxazolidinedione compounds, cyanoacetamide compounds, phenylamide compounds, cinnamic acid compounds, and organophosphorus compounds.

In addition to the fungicides that have been named, other fungicides for Phycomycetes having at least one of a preventive effect, a curative effect and penetrability are also expected to produce the same effects as observed in the present invention. For example, dithiocarbamate fungicides can be mentioned as an example of fungicides for Phycomycetes having only a preventive effect.

The compositions for controlling harmful bioorganisms according to the present invention which comprises at least one imidazole compound of formula (I) as active ingredient and at least one inorganic phosphorus compound as active ingredient are particularly suitable for agricultural and horticultural uses. Specifically, they exhibit excellent effects of controlling diseases of crop plants, such as rice blast caused by Pyricularia oryzae, rice sheath blight caused by Rhizoctonia solani, cucumber anthracnose caused by Colletotrichum lagenarium, cucumber powdery mildew caused by Sphaerotheca fuliginea, cucumber downy mildew caused by Pseudoperonospora cubensis, tomato late blight caused by Phytophthora infestans, tomato early blight caused by Alternaria solani, citrus melanose caused by Diaporthe citri, citrus common green mold caused by Penicillium digitatum, pear scab caused by Venturia nashicola, apple Alternaria blotch caused by Alternaria mali, grape downy mildew caused by Plasmopara viticola, gray mold caused by Botrytis cinerea, Sclerotinia rot caused by Sclerotinia sclerotiorum, and disease caused by rust, etc.; and soil diseases caused by phytopathogenic fungi, such as Fusarium, Pythium, Rhizoctonia, Verticillium, and Plasmodiophora, etc. In particular, the compositions of the present invention exhibit excellent effects of controlling diseases such as potato late blight caused by Phytophthora infestans, sweet pepper Phytophtora blight caused by Phytophthora capsici, watermelon Phytophthora rot caused by Phytophthora drechsleri, tobacco black shank caused by Pbytophthora nicotianae var. nicotianae, tomato late blight caused by Phytophthora infestans, cucumber or melon downy milder caused by Pseudoperonospora cubensis, cabbages or Chinese cabbages downy mildew caused by Peronospora brassicae, onion downy mildew caused by Peronospora destructor, onion shiroiro-eki-byo caused by Pbytophthora porri and watermelon brown rot caused by Phytophthora capsici, and grape downy mildew caused by Plasmopara viticola and various soil diseases caused by e.g. Aphanomyces, Pythium. The compositions have a prolonged residual effect and exhibit a particularly excellent curative effect. It is therefore possible to control diseases by treatment after infection. In addition, since the compositions possess a systemic activity, it is possible to control diseases of stems and foliage by soil treatment.

The compositions for controlling harmful bioorganisms according to the present invention which comprises at least one imidazole compound of formula (I) as active ingredient and a fungicide for Phycomycetes as active ingredient have excellent fungicidal activities when applied to crop plants, for example, fruit vegetables cucumbers, tomatoes, eggplants, etc.); cereals rice, wheat, etc.); seed vegetables; fruits apples, pears, grapes, citrus, etc.); potatoes, etc., which have been infected, or suspected of being infected, with pathogenic fungi.

They exhibit excellent controlling effects on diseases, such as powdery mildew, downy mildew, anthracnose, gray mold, common green mold, Sclerotinia rot, scab, Alternaria blotch, bacterial spot, black spot, melanose, ripe rot, late blight, early blight, blast, sheath blight, damping-off, southern blight, etc. The compositions also exert excellent controlling effects on soil diseases caused by Phycomycetes, such as Pythium, and other plant pathogens, such as Fusarium, Rhizoctonia, Verticillium, Plasmodiophora, etc. The compositions have a prolonged residual effect and exhibit a particularly excellent curative effect. It is therefore possible to control diseases by treatment after infection. In addition, since the compositions possess a systemic activity, it is possible to control diseases of stems and foliage by soil treatment.

In particular, the compositions comprising at least one imidazole compound of formula as active ingredient and a copper compound and/or an organophosphorus compound as a fungicide for Phycomycetes as active ingredient are particularly useful in agriculture and horticulture. Specifically, the compositions exhibit excellent effects of controlling diseases of crop plants, such as rice blast caused by Pyricularia oryzae, rice sheath blight caused by Rhizoctonia solani, cucumber anthracnose caused by Colletotrichum lagenarium, cucumber powdery mildew caused by Sphaerotheca fuliginea, cucumber downy mildew caused by Pseudoperonospora cubensis, tomato late blight caused by Phytophthora infestans, tomato early blight caused by Alternaria solani, citrus melanose caused by Diaporthe citri, citrus common green mold caused by Penicillium digitatum, pear scab caused by Venturia nashicola, apple Alternaria blotch caused by Alternaria mali, grape downy mildew caused by Plasmopara viticola, gray mold caused by Botrytis cinerea, sclerotinia rot caused by Sclerotinia sclerotiorum, rust, bacterial spot, etc.; and soil diseases caused by phytopathogenic fungi, such as Fusarium, Pythium, Rhizoctonia, Verticillium, Plasmodiophora, etc. In particular, the compositions exhibit excellent effects of controlling diseases such as potato or tomato late blight caused by Phytophthora infestans, cucumber downy mildew caused by Pseudoperonospora cubensis, grape downy mildew caused by Plasmopara viticola; and various soil diseases caused by Phycomycetes, such as Plasmodiophora, Aphanomyces, Pythium, etc.

The compositions of the present invention have a prolonged residual effect so that they exhibit an excellent preventive effect, and also exhibit an excellent curative effect as well. It is therefore possible to control diseases by treatment after infection. In addition, since they possess a systemic activity, it is also possible to control diseases of the stem and leaf by soil treatment.

In particular, the compositions containing a copper compound as a fungicide for Phycomycetes exhibit an excellent preventive effect, and the compositions containing an organophosphorus compound as a fungicide for Phycomycetes exhibit an.excellent curative effect.

The compositions for controlling harmful bioorganisms comprising at least one imidazole compound of formula as active ingredient and a cyanoacetamide compound, a phenylamide compound or a cinnamic acid compound as a fungicide for Phycomycetes as active ingredient exhibit excellent controlling effects on diseases caused by Phycomycetes, such as plant diseases, downy mildew of cucumbers, melons, cabbages, Chinese cabbages, onions, pumpkins, and grapes; late blight of potatoes, red peppers, sweet peppers, watermelons, pumpkins, tobaccos, and tomatoes; onion shiroiro-eki-byo; watermelon brown rot; soil diseases caused by plant pathogenic fungi, such as Pythium, etc. It also has excellent controlling effects on diseases caused by Plasmodiophora.

The compositions for controlling harmful bioorganisms comprising at least one imidazole compound of formula as active ingredient and a pmethoxyacrylate compound, an oxazolidinedione compound or an organic chlorine compound as a fungicide for Phycomycetes as active ingredient exhibit excellent controlling effects against diseases caused by Phycomycetes, such as plant diseases, rice blast; rice sheath blight; cucumber anthracnose; downy mildew of cucumbers, melons, cabbages, Chinese cabbages, onions, pumpkins, and grapes; powdery mildew of wheat, barley and cucumbers; late blight of potatoes, red peppers, sweet peppers, watermelons, pumpkins, tobaccos, and tomatoes; wheat speckled leaf blotch; tomato early blight; citrus melanose; citrus common green mold; pear scab; apple Alternaria blotch; onion shiroiro-eki-byo; watermelon brown rot; various diseases such as gray mold, Sclerotinia rot, rust, and bacterial spot; various soil diseases caused by plant pathogenic fungi, etc., such as Fusarium, Pythium, Rhizoctonia, Verticillium, etc. It also has excellent controlling effects on diseases caused by Plasmodiophora. The compositions show particularly excellent controlling effects on diseases such as Phytophthora rot of potatoes, red peppers, sweet peppers, watermelons, pumpkins, tobaccos, tomatoes, etc.; and downy mildew of cucumbers, melons, cabbages, Chinese cabbages, onions, pumpkins, grapes, etc.

Further, the compositions comprising active ingredients and of the present invention show an excellent controlling effect against agriculturally and horticulturally harmful insects, such as planthoppers (Delphacidae) diamondback moth (Plutella xylostella) green rice leafhopper (Nephotettix cincticeps) adzuki bean weevil (Callosobruchus chinensis) common cutworm (Spodoptera litura) green peach aphid (Myzus persicae) etc.; mites, such as two-spotted spider mite (Tetranychus urticae) carmine spider mite (Tetranychus cinnabarinus) citrus red mite (Panonychus citri), etc.; and nematodes, such as southern root-knot nematode (Meloidogyne incognita), etc.

The compositions for controlling harmful bioorganisms comprising active ingredient and activityenhancing ingredient are particularly suitable for agricultural and horticultural uses. The harmful bio-organisms which can be controlled by the compositions include plant pathogenic fungi causing plant diseases, such as rice blast; rice sheath blight; cucumber anthracnose; cucumber powdery mildew; downy mildew of cucumber, melon, cabbage, Chinese cabbage, onion and grape; late blight of potato, red pepper, sweet pepper, watermelon, pumpkin, tobacco; tomato Phytophthora rot; tomato early blight; citrus melanose; citrus common green mold; pear scab; apple Alternaria blotch; various plant diseases such as gray mold, Sclerotinia rot, rust, etc.; soil borne pathogenic fungi causing various plant diseases, such as Fusaium, Pythium, Rhizoctonia, Verticillium, Plasmodiophora, etc.; insects, such as planthoppers, diamondback moth, green rice leafhopper, adzuki bean weevil, common cutworm, green peach aphid, etc.; mites, such as twospotted spider mite, carmine spider mite, citrus red mite, etc.; and nematodes, such as southern root-knot nematode, etc. More specifically, they are effective on Phytophthora rot of potatoes, red peppers, sweet peppers, watermelons, pumpkins, tobaccos, and tomatoes and downy mildew of cucumbers, melons, cabbages, Chinese cabbages, onions, pumpkins, and grapes. The compositions comprising active ingredient and activity-enhancing ingredient have a prolonged residual effect and exhibit not only an excellent preventive effect but an excellent curative effect. It is therefore possible to control diseases by treatment after infection.

The active ingredients, inclusive of other pesticides hereinafter described as well as active ingredients and and activity-enhancing ingredient which constitute the compositions for controlling harmful bio-organisms can be formulated into a variety of forms, such as emulsifiable concentrates, dusts, wettable powders, aqueous solutions, granules, suspension concentrates, etc., together with various adjuvants, as in conventional agricultural preparations. Active ingredient (the imidazole compound of formula active ingredient and other specific compounds may be mixed and formulated, or each of them may be be mixed and formulated, or each of them may be separately formulated and then mixed together. Upon use, the preparation may be used as such or as diluted with an appropriate diluent, water, to a predetermined concentration.

Examples of the adjuvants which can be used include carriers, emulsifying agents, suspending agents, thickeners, stabilizers, dispersants, spreaders except those used as activity-enhancing ingredient surface active agents, wetting agents, penetrating agents, antifreezing agents, antifoaming agents, etc. These adjuvants are added appropriately according to necessity.

The carriers are classified into solid carriers and liquid carriers. The solid carriers include animal and vegetable powders (e g starch, sugar, cellulose powders, cyclodextrin, activated charcoal, soybean powders, wheat powders, chaff powders, wood powders, fish powders, powdery milk, etc.) and mineral powders talc, kaolin, bentonite, bentonite-alkylamine complexes, calcium carbonate, calcium sulfate, sodium hydrogencarbonate, zeolite, diatomaceous earth, white carbon, clay, alumina, silica, sulfur powder, slaked lime, etc.). Examples of the liquid carriers include water, vegetable oils soybean oil, cotton seed oil), animal oils beef tallow, train oil, etc.), alcohols ethyl alcohol, ethylene glycol, etc.), ketones acetone, methyl ethyl ketone, methyl isobutyl ketone, isophorone, etc.), ethers dioxane, tetrahydrofuran, etc.), aliphatic hydrocarbons kerosene, lamp oil, liquid paraffin, etc.), aromatic hydrocarbons toluene, xylene, trimethylbenzene, tetramethylbenzene, cyclohexane, solvent naphtha, etc.), halogenated hydrocarbons chloroform, chlorobenzene, etc.), acid amides dimethylformamide, etc.), esters ethyl acetate, fatty acid glycerine esters, etc.), nitriles acetonitrile, etc.), sulfur-containing compounds dimethyl sulfoxide, etc.), N-methyl-2-pyrrolidone, N,Ndimethylformamide and so on. The spreaders (except those used as activity-enhancing ingredient or surface active agents include polyoxyethylene sorbitan fatty acid esters.

In the compositions comprising at least one imidazole compound of formula as active ingredient and at least one inorganic phosphorus compound as active ingredient the weight ratio of to is usually 1:300 to 300:1, preferably 1:100 to 100:1, still preferably 1:50 to 5:1, most preferably 1:50 to 1:10.

In the compositions comprising at least one imidazole compound of formula as active ingredient and at least one fungicide for Phycomycetes as active ingredient the weight ratio of to is usually 1:10000 to 10000:i, preferably 1:1000 to 10000:1, still preferably 1:100 to 1000:1. Especially in the compositions containing at least one imidazole compound of formula as active ingredient and at least one cyanoacetamide compound as active ingredient (b) (fungicide for phycomycetes) is preferably 1:300 to 5:1.

Where, in particular, copper compounds and/or organophosphorus compounds are used as active ingredient (fungicide for Phycomycetes) the weight ratio of to is usually 1:2000 to 2000:1, preferably 1:300 to 300:1, still preferably 1:100 to 100:1, particularly preferably 1:50 to 5:1.

In the compositions containing active ingredient and activity-enhancing ingredient the weight ratio of to is usually 1:5000 to 2000:1, preferably 0.05:99.95 to 90:10, still preferably 0.2:99.8 to 80:20.

A method for controlling harmful bio-organisms comprising applying the compositions for controlling harmful bio-organisms of the prevent invention is also included under the scope of the present invention.

In using the compositions for controlling harmful bio-organisms comprising at least one imidazole compound of formula as active ingredient and at least one inorganic phosphorus compound as active ingredient the concentrations of use of the active ingredients (a) and cannot be generally defined because they vary depending on, for example, the crop plant to be treated, the method of treatment, the form of the preparation, and the amount of the preparation to be applied. For example, the imidazole compound of formula and the inorganic phosphorus compound are used in concentrations of 1 to 1000 ppm and 1 to 5000 ppm, respectively, in foliar treatment, and 10 to 10,000 g/ha and 10 to 50,000 g/ha, respectively, in soil treatment.

In using the compositions for controlling harmful bio-organisms comprising at least one imidazole compound of formula as active ingredient and at least one fungicide for Phycomycetes selected from the group consisting of a p-methoxyacrylate compound, an oxazolidinedione compound, a cyanoacetamide compound, an organic chlorine compound, a phenylamide compound, and a cinnamic acid compound as active ingredient the concentrations of use of the active ingredients cannot be generally defined because they vary depending on the kind of the fungicide used, the crop plant to be treated, the method of treatment, the form of the preparation, the amount of the preparation to be applied, the timing of treatment, and the kind of the harmful fungi to be controlled. For foliar treatment, for example, the imidazole compound of formula and the fungicide are used in concentrations of 0.01 to 1000 ppm and 0.01 to 1000 ppm, respectively, preferably 0.1 to 500 ppm and 0.1 to 500 ppm, respectively.

In using the compositions for controlling harmful bio-organisms comprising at least one imidazole compound of formula as active ingredient and a copper compound and/or an organophosphorus compound as active ingredient the concentrations of use of the active ingredients cannot be generally defined because they vary depending on, for example, the kind of the fungicide used, the crop plant to be treated, the method of treatment, the form of the preparation, the amount of the preparation to be applied, the timing of treatment, and the kind of the harmful fungi to be controlled. For example, the imidazole compound of formula and the fungicide are used in concentrations of 0.01 to 1000 ppm and 1 to 5000 ppm, respectively, in foliar treatment and to 10,000 g/ha and 10 to 50,000 g/ha, respectively, in soil treatment.

In using the compositions comprising active ingredient and activity-enhancing ingredient the concentrations of use of these ingredients cannot be generally defined because they vary depending on, for example, the crop plant to be treated, the method of treatment, the form of the preparation, and the amount of the preparation to be applied. For example, active ingredient and activity-enhancing ingredient are used in concentrations of 0.1 to 10,000 ppm and 0.01 to ppm, respectively, in foliar treatment and 0.01 to 100 kg/ha and 0.1 to 0.5 kg/ha, respectively, in soil treatment.

The compositions comprising active ingredients and can be used as a mixture or in combination with, for example, other pesticides, fertilizers, and safeners, to exhibit enhanced effects and actions.

Useful pesticides include bactericides except those used as active ingredients and fungicides, insecticides, acaricides, nematicides, antiviral agents, attaractants, herbicides, and plant growth regulators.

In particular, mixtures or combinations of the compositions for controlling harmful bio-organisms and one or more active ingredients of fungicides other than those used as active ingredients and can enjoy enhancements, for example, in terms of the range of controllable harmful bio-organisms, the timing of treatment, and the controlling activity on harmful bio-organisms. The imidazole compound of formula as active ingredient the inorganic phosphorus compound and/or fungicide for Phycomycetes as active ingredient and the active ingredient(s) of fungicides other than those used as active ingredients and can be separately formulated and mixed together on use, or one or at least two of them can be mixed and formulated into a single preparation.

Where at least one imidazole compound of formula as active ingredient is combined with at least one of the inorganic phosphorus compound and/or at least one of the fungicides for phycomycetes as active ingredient a composition prepared immediately before use manifests further enhanced controlling effects over a previously prepared composition.

Therefore, it is convenient that a composition containing active ingredient and, if desired, various adjuvants and a composition containing active ingredient and, if desired, various adjuvants are separately packed and supplied as a two-pack preparation.

For example, active ingredient and active ingredient can be dissolved in respective liquid carriers and packed separately, or active ingredient and a mixture of active ingredient and other fungicides are dissolved in respective liquid carriers and packed separately.

In the harmful bio-organism controlling method using the compositions comprising active ingredient (a) and activity-enhancing ingredient the compositions can be used as a mixture with the above-described other pesticides, which can bring about further enhanced effects. Typical examples of useful other pesticides include azole compounds such as Triflumizole (common name), etc.; quinoxaline compounds such as Quinomethionate (common name) etc.; benzimidazole compounds such as Benomyl (common name) etc. pyridinamine compounds such as Fluazinam (common name), etc.; sulfenic acid compounds such as Dichlofluanid (common name), etc.; isoxazole compounds such -as Hydroxyisoxazole (common name), etc.; dicarboxyimide compounds such as Procymidone (common name) etc.; benzanilide compounds such as Flutolanil (common name), etc.; and benzamide compounds such as (R,S)-4-chloro-N- [cyano(ethoxymethyl]benzamide, etc.

Preferred embodiments of the compositions for controlling harmful bio-organisms described herein which comprise active ingredients (a) and are shown below for illustrative purposes only but not for limitation.

The compositions for controlling harmful bioorganisms wherein at least one inorganic phosphorus compound and/or at least one have a preventive effect.

The compositions for organisms wherein at least compound and/or at least one have a curative effect.

The compositions for organisms wherein at least compound and/or at least one have penetrability.

The compositions for organisms wherein at least compound and/or at least one have a preventive effect and The compositions for organisms wherein at least compound and/or at least one have a preventive effect and The compositions for organisms wherein at least compound and/or at least one fungicide for Phycomycetes controlling harmful bioone inorganic phosphorus fungicide for Phycomycetes controlling harmful bioone inorganic phosphorus fungicide for Phycomycetes controlling harmful bioone inorganic phosphorus fungicide for Phycomycetes a curative effect.

controlling harmful bioone inorganic phosphorus fungicide for Phycomycetes penetrability.

controlling harmful bioone inorganic phosphorus fungicide for Phycomycetes have a curative effect and penetrability.

The compositions for controlling harmful bioorganisms wherein at least one inorganic phosphorus compound and/or at least one fungicide for Phycomycetes have a preventive effect, a curative effect and penetrability.

The compositions for controlling harmful bioorganisms wherein active ingredient is at least one inorganic phosphorus compound.

The compositions for controlling harmful bioorganisms wherein active ingredient is at least one fungicide for Phycomycetes.

The compositions for controlling harmful bioorganisms according to above, wherein the fungicide for Phycomycetes is a compound selected from the group consisting of a p-methoxyacrylate compound, an oxazolidinedione compound, a cyanoacetamide compound, an organic chlorine compound, a phenylamide compound, a cinnamic acid compound, a copper compound, and an organophosphorus compound.

(11) The compositions for controlling harmful bioorganisms according to above, wherein the fungicide for Phycomycetes is a p-methoxyacrylate compound and/or an oxazolidinedione compound.

(12) The compositions for controlling harmful bioorganisms according to above, wherein the fungicide for Phycomycetes is a compound selected from the group consisting of a cyanoacetamide compound, an organic chlorine compound, a phenylamide compound, a cinnamic acid compound, a copper compound, and an organophosphorus compound.

(13) The compositions for controlling harmful bioorganisms according to (10) or (11) above, wherein the fungicide for Phycomycetes is a P-methoxyacrylate compound.

(14) The compositions for controlling harmful bioorganisms according to wherein the p-methoxyacrylate compound is methyl (E)-2-{2-[6-(2-cyanophenoxy)pyrimidin-4-yloxy]phenyl)-3methoxyacrylate or methyl -methoxyimino (otolyloxy)-O-tolyl]acetate.

The compositions for controlling harmful bioorganisms according to (10) or wherein the fungicide for Phycomycetes is an oxazolidinedione compound.

(16) The compositions for controlling harmful bioorganisms according to (15) wherein the fungicide for Phycomycetes is 3-anilino-5-methyl-5-(4-phenoxyphenyl)- 1,3-oxazolidine-2,4-dione.

(17) The compositions for controlling harmful bioorganisms according to (10) or wherein the fungicide for Phycomycetes is a cyanoacetamide compound.

(18) The compositions for controlling harmful bioorganisms according to (17) wherein the cyanoacetamide compound is 1- 2 -cyano-2-methoxyiminoacetyl) -3-ethylurea.

(19) The compositions for controlling harmful bioorganisms according to (10) or wherein the fungicide for Phycomycetes is an organic chlorine compound.

The compositions for controlling harmful bioorganisms according to (19) wherein the organic chlorine compound is tetrachloroisophthalonitrile or pentachloronitrobenzene.

(21) The compositions for controlling harmful bioorganisms according to (19) wherein the organic chlorine compound is tetrachloroisophthalonitrile.

(22) The compositions for controlling harmful bioorganisms according to (10) or wherein the fungicide for Phycomycetes is a phenylamide compound.

(23) The compositions for controlling harmful bioorganisms according to wherein the phenylamide compound is at least one compound selected from the group consisting of methyl N- (2-methoxyacetyl) (2,6xylyl) -DL-alaninate, 2-methoxy-N- (2-oxo-1, 3-oxazolidin- 3-yl)aceto-2',6'-xylidide, -a-2-chloro-N-(2,6xylylacetamide) -y-butyrolactone, methyl N-phenylacetyl- N-(2,6-xylyl)-DL-alaninate, methyl N- (2-furoyl) (2,6xylyl)-DL-alaninate, and (3-chlorophenyl) cyclopropanecarboxamide]-y-butyrolactone.

(24) The compositions for controlling harmful bioorganisms according to wherein the phenylamide compound is methyl N-(2-methoxyacetyl)-N-(2,6-xylyl)-DLalaninate.

The compositions for controlling harmful bioorganisms according to (10) or (12) wherein the fungicide for Phycomycetes is a cinnamic acid compound.

(26) The compositions for controlling harmful bioorganisms according to (25) wherein the cinnamic acid compound is (E,Z)-4-[3-(4-chlorophenyl)-3-(3,4dimethoxyphenyl)acryloyl]morpholine.

(27) The compositions for controlling harmful bioorganisms according to (10) or (12) wherein the fungicide for Phycomycetes is a copper compound and/or an organophosphorus compound.

(28) The compositions for controlling harmful bioorganisms according to wherein the copper compound is an inorganic copper fungicide and/or an organic copper fungicide.

(29) The compositions for controlling harmful bioorganisms according to wherein the active ingredient of the inorganic copper fungicide is at least one member selected from the group consisting of cupric hydroxide, copper oxysulfate, copper oxychloride, anhydrous copper (II) sulfate, and basic copper calcium sulfate.

The compositions for controlling harmful bioorganisms according to (27) wherein the organophosphorus compound is at least one member selected from the group consisting of aluminum tris(ethyl phosphonate), 0-2,6-dichloro-p-tolyl-O,0dimethyl phosphorothioate, S) -sec-butyl-0ethyl-2-oxo-2-thiazolidinyl phosphonothioate, S-benzyl diisopropyl phosphorothioate, O-ethyl diphenyl phosphorodithioate, and ethyl 2-diethoxythiophosphoryloxy-5-methylpyrazolo(1,5-a)pyrimidine-6-carboxylate.

(31) The compositions for controlling harmful bioorganisms according to wherein the organophosphorus compound is aluminium tris(ethyl phosphonate).

(32) The compositions for controlling harmful bioorganisms according to wherein the weight ratio of at least one imidazole compound of formula to the copper compound and/or the organophosphorus compound is 1:2000 to 2000:1.

Preferred embodiments of applying the composition containing active ingredient and activity-enhancing ingredient to harmful bio-organisms are described below for illustrative purposes only but not for limitation.

The compositions containing active ingredient (a) and activity-enhancing ingredient can be applied to harmful bio-organisms in the form of an aqueous dispersion. In this method, the aqueous dispersion is sprayed over the sites where a harmful bio-organism has occurred or is expected to occur, such as foliage of useful plants or soil. The aqueous dispersion is particularly effective for application to foliage. The aqueous dispersion is prepared, for example, by dispersing a preparation of the active ingredient in water and adding thereto the activity-enhancing ingredient; (ii) dispersing a preparation containing the active ingredient and the activity-enhancing ingredient in water; or the method similar to or (ii) The aqueous dispersion to be applied is prepared by using 1 liter of water per 0.1 to 10,000 mg of the compositions for controlling harmful bio-organisms so as to have the active ingredient in a concentration of 0.1 to 10,000 ppm. The aqueous dispersion is sprayed in an amount of 100 to 10,000 1/ha.

The compositions containing active ingredient (a) and activity-enhancing ingredient can be applied in the form of an aqueous suspension in the same manner as for the aqueous dispersion. The concentration of the active ingredient in the aqueous suspension is 0.1 to 10,000 ppm. The aqueous suspension is sprayed in an amount of 100 to 10,000 1/ha.

Test Examples of the compositions for controlling harmful bio-organisms described herein in usage as an agricultural or horticultural fungicide are given below for illustrative purposes.

Test Example 1 Test of Curative Effect on Cucumber Downy Mildew A composition for controlling harmful bioorganisms containing Compound No. 1 and the inorganic phosphorus compound shown in Table 9 below in a concentration of 100 ppm and 2000 ppm, respectively, was prepared by mixing an aqueous suspension concentrate of Compound No. 1 and a 20% wettable powder of the inorganic phosphorus compound. The 20% wettable powder of the inorganic phosphorus compound was prepared in accordance with Reference Formulation Example hereinafter given.

A cucumber (cultivars: Suyo) was cultivated in polyethylene pots (diameter: 7.5 cm). When the plant reached a two-leaf stage, it was inoculated by spraying a spore suspension of fungi of downy mildew (Pseudoperonospora cubensis). After 24 hours, 10 ml/pot of the above-prepared composition was sprayed onto the plant by means of a spray gun. For comparison, the same test was carried out by using 10 ml of a composition containing 2000 ppm of the inorganic phosphorus compound and containing no Compound No. 1 or 10 ml of a composition containing 100 ppm of Compound No. 1 and containing no inorganic phosphorus compound. The plant was kept in a chamber set at 22 to 24 0 C for 6 days, and the lesion area of the first leaf was measured, from which the disease incidence rate was calculated according to the following formula. The results obtained are shown in Table 9.

Incidence rate x 100 wherein a is a lesion area of a treated plant; and b is a lesion area of a control (non-treated plant).

A theoretical incidence rate can be calculated from the following Colby's formula. In cases where an incidence rate of a tested composition is lower than the theoretical one, the tested composition can produce a synergistic effect. In these cases, the theoretical incidence rate is shown in parentheses in Table 9.

Theoretical incidence rate (X1 x Y)/100 wherein X' is an incidence rate of a plant treated with only Compound No. 1; and Y1 is an incidence rate of a plant treated with only the inorganic phosphorus compound.

Table 9 Curative Effect on Cucumber Downy Mildew (Incidence Rate: Inorganic Phosphorus Compound No. 1 Compound, 2000 ppm 100 ppm 0 ppm Na3PO4-12H 2 0 0 (90.2) A (H 2 P4) 3 5 (95) 100 H2(PO 3 H) 12.5 (71.3) Na 2 HPO3-5H 2 0 0 (85.5)

K

2 HPO4 2.5 (90.2) Na 2

HPO

4 2.5 (95) 100 100 none 95 (one control) Test Example 2 Test of Curative Effect on Cucumber Downy Mildew A composition for controlling harmful bioorganisms containing Compound No. 1 in a prescribed concentration and the inorganic phosphorus compound shown in Table 10 below in a concentration of 250 ppm was prepared by mixing an aqueous suspension concentrate of Compound No. 1 and a 20% wettable powder of the inorganic phosphorus compound. The 20% wettable powder of the inorganic phosphorus compound was prepared in accordance with Reference Formulation Example hereinafter given.

A cucumber (cultivars: Suyo) was cultivated in polyethylene pots (diameter: 7.5 cm). When the plant reached a two-leaf stage, it was inoculated by spraying a spore suspension of fungi of downy mildew (Pseudoperonospora cubensis). After 24 hours, 10 ml/pot of the above-prepared composition was sprayed onto the plant by means of a spray gun. For comparison, the same test was carried out by using 10 ml/pot of a composition containing 250 ppm of the inorganic phosphorus compound and containing no Compound No. 1 or 10 ml/pot of a composition containing Compound No. 1 at a prescribed concentration and containing no inorganic phosphorus compound. The plant was kept in a chamber set at 22 to 24 0 C for 4 days, and the lesion area of the first leaf was measured, from which the disease incidence rate was calculated according to the following formula. The results obtained are shown in Table Incidence rate x 100 wherein a is a lesion area of a treated plant; and b is a lesion area of a control (non-treated plant).

A theoretical incidence rate can be calculated from the following Colby's formula. In cases where an incidence rate of a tested composition is lower than the theoretical one, the tested composition can produce a synergistic effect. In these cases, the theoretical incidence rate is shown in parentheses in Table Theoretical incidence rate (X 2 x Y 2 )/100 wherein X 2 is an incidence rate of a plant treated with only Compound No. 1; and Y 2 is an incidence rate of a plant treated with only the inorganic phosphorus compound.

Table Curative Effect on Cucumber Downy Mildew (Incidence Rate: Compound No. 1 Inorganic Phosphorus Compound No. 1 Compound, 250 ppm 50 ppm 12.5 ppm 0 ppm Na 3 PO4-12H 2 0 0 (77) 3 (81) Al(H 2

PO

4 3 0 (85) 0 (90) 100

H

2

(PO

3 H) 0 (64) 3 (68) Na 2 HPO3-5H 2 0 0 (72) 3 (77)

K

2

HPO

4 0 (81) 5 (86) Na 2

HPO

4 3 (85) 3 (90) 100 100 none 85 90 (control) (control) Test Example 3 Field Test of Effect on Cucumber Downy Mildew Five cucumber seedlings (cultivars: Tokiwa Kohai Hikari No. 3, P type) in the two-leaf stage were planted in a divided area (3 m 2 each) of the field located in Kusatsu City, Shiga, Japan on May 10, 1997. A composition containing 50 ppm of Compound No. 1 and 1500 ppm of an inorganic phosphorus compound shown in Table 11 below was sprayed in an amount of 500 ml per area by means of a small-sized spraying machine on June and 17. For comparison, the same field test was carried out by using a composition containing only 1500 ppm of the inorganic phosphorus compound or a composition containing only 50 ppm of Compound No. 1.

On June 23, all the leaves were observed to obtain a control index in accordance with the following rating system. The results obtained are shown in Table 11.

Artificial infection with a pathogenic fungus was not conducted so that the disease was spontaneous.

Control Severity of Disease Index The lesion area or length is less than 3% of that of a control (non-treated area).

The lesion area or length is 3% or more and less than 5% of that of the control.

The lesion area or length is 5% or more and less than 10% of that of the control.

The lesion area or length is 10% or more and less than 30% of that of the control.

The lesion area or length is 30% or more of that of the control.

Table 11 Field Test on Cucumber Downy Mildew (Control Index) Inorganic Phosphorus Compound, Compound No. 1 1500 ppm 50 ppm 0 ppm Alexin 95 PS* 5 1 Phytex 200 SL" 5 1 none 3 1 Note: An aqueous solution having an phosphorous acid concentration of 600 g/l, available from Masso.

An aqueous solution having a phosphorous acid concentration of 200 g/l, available from Horticura cc).

Test Example 4 Test of Curative Effect on Cucumber Downy Mildew A cucumber (cultivars: Suyo) was cultivated in polyethylene pots (diameter: 7.5 cm) When the plant reached a two-leaf stage, it was inoculated by spraying a spore suspension of fungi of downy mildew (Pseudoperonospora cubensis). After 24 hours, 10 ml of a composition containing the compounds shown in Tables 12 to 19 in respective concentrations shown was sprayed onto the plant by means of a spray gun. The plant was kept in a chamber set at 22 to 24 0 C for 6 days, and the lesion area of the first leaf was measured, from which the disease incidence rate was calculated according to the following formula. The results obtained are shown in Tables 12 to 19.

Incidence rate x 100 wherein a is a lesion area of a treated plant; and b is a lesion area of a control (non-treated plant).

A theoretical incidence rate can be calculated from the following Colby's formula. In cases where an incidence rate of a tested composition is lower than the theoretical one, the tested composition can produce a synergistic effect. In these cases, the theoretical incidence rate is shown in parentheses in Tables 12 to 19.

Theoretical incidence rate (X 3 x Y 3 )/100 wherein X 3 is an incidence rate of a plant treated with only Compound No. 1, 2 or 3; and Y3 is an incidence rate of a plant treated with only compound methyl (E)-2-{2-[6-(2-cyanophenoxy)pyrimidin- 4-yloxy]phenyl}-3-methoxyacrylate) compound methyl -methoxyimino (o-tolyloxy) -O-tolyl] acetate) Cymoxanil, Metalaxyl or Dimethomorph.

Table 12 Curative Effect on Cucumber Downy Mildew (Incidence Rate; Compound (a) Compound No. 1 63 ppm 2 ppm 0 ppm 500 ppm 0 5 10) 125 ppm 5 0 5) 31 ppm 5 5 50) 8 ppm 0 5 (100) 100 0 ppm 5 100 (100) 100 Curative Effect on Cucumber Downv Mildew (Tnreirlnr.0 'R c: 9fsl

-L

Compound (b) Compound No. 1 500 ppm 125 ppm 0 ppm 500 ppm 10 0 10) 125 ppm 0 5) 0 5) 31 ppm 10 (50) 0 50) 8 ppm 10 (100) 10 (100) 100 0 ppm 100 100 100 Table 14 Curative Effect on Cucumber Downy Mildew (Incidence Rate; Compound (a) Compound No. 3 ppm 2 ppm 0 ppm 125 ppm 5 (10) 31 ppm 5 (10) 8 ppm 10 (50) 2 ppm 50 0 ppm 100 100 Table Curative Effect on Cucumber Downy Mildew fTnr r4 1 ntM& c rr Compound (b) Compound No. 3 500 ppm 125 ppm 0 ppm 125 ppm 5 (10) 10 31 ppm 5 (10) 5 (10) 8 ppm 5 (50) 10 (50) 2 ppm 50 10 (50) 0 ppm 100 100 100 Table 16 Curative Effect on Cucumber Downy Mildew (Incidence Rate; Compound Cymoxanil No. 1 31 ppm 8 ppm 2 ppm 0 ppm 8 ppm 0 7) 15 70) 85 2 ppm 2 (10) 15 (100) 85 (100) 100 ppm 10 50 (100) 70 (100) 100 0.125 ppm 7 (10) 100 100 100 0 ppm 10 100 100 100 Table 17 Curative Effect on Cucumber Downy Mildew (Incidence Rate: Cymoxanil Compound No. 2 31 ppm 0 ppm 125 ppm 5 7) 31 ppm 5 7) 8 ppm 5 (35) 2 ppm 5 (70) 100 0 ppm 70 100 Table 18 Curative Effect on Cucumber Downy Mildew (Incidence Rate; Metalaxyl Compound No. 1 2 ppm 0.5 ppm 0 ppm 8 ppm 20 (42) 10 70) 2 ppm 7 (60) 70 (100) 100 ppm 35 (60) 85 (100) 100 0.125 ppm 70 100 100 0 ppm 60 100 100 Table 19 Curative Effect on Cucumber Downy Mildew (Incidence Rate; Dimethomorph Compound No. 1 31 ppm 8 ppm 0 ppm 8 ppm 0 (35) 4 (42) 2 ppm 4 (50) 50 (60) 100 ppm 20 (50) 85 100 0 ppm 50 60 100 Test Example Test of Curative Effect on Cucumber Downy Mildew A cucumber (cultivars: Suyo) was cultivated in polyethylene pots (diameter: 7.5 cm) When the plant reached a two-leaf stage, it was inoculated by spraying a spore suspension of fungi of downy mildew (Pseudoperonospora cubensis). After 18 hours, 20 ml of a composition containing Compound No. 1 and compound (c) (3-anilino-5-methyl-5-(4-phenoxyphenyl)-1,3-oxazolidine- 2,4-dione) in respective concentrations shown in Table was sprayed on two seedlings by means of a spray gun.

The plants were kept in a chamber set at 22 to 24 0 C for days, and the average lesion area of the two seedlings was obtained, from which the disease incidence rate was calculated in the same manner as in Test Example 1.

The results obtained are shown in Table A theoretical incidence rate can be calculated from the following Colby's formula. In cases where an incidence rate of a tested composition is lower than the theoretical one, the tested composition can produce a synergistic effect. In these cases, the theoretical incidence rate is shown in parenthesis in Table Theoretical incidence rate (X 4 x Y 4 )/100 wherein X 4 is an incidence rate of a plant treated with only Compound No. 1; and Y 4 is an incidence rate of a plant treated with only compound Table Curative Effect on Cucumber Downy Mildew (Incidence Rate: Compound Compound (c) No. 1 800 ppm 400 ppm 200 ppm 0 ppm 200 ppm 0 0 (13) 0 (48) 87 100 ppm 0 0 (14) 0 (52) ppm 0 0 (14) 0 (52) 0 ppm 5 15 55 100 Test Example 6 Test of Curative Effect on Tomato Late Blight A tomato (cultivar: Ponderosa) was cultivated in polyethylene pots (diameter: 7.5 cm). When the plant reached a four-leaf stage, it was inoculated by spraying a zoosporangium suspension of fungi of late blight (Phytophthora iafestans). After 6 hours, 10 mil/pot of a composition containing Compound No. 1 and Cymoxanil, Metalaxyl or Dimethomorph in the respective concentrations shown in Tables 21 to 23 was sprayed on the plant by means of a spray gun. The plant was kept in a chamber set at 22 to 24°C for 3 to 5 days, and the lesion area was measured, from which the disease incidence rate was calculated in the same manner as in Test Example 1. The results obtained are shown in Tables 21 to 23.

A theoretical incidence rate can be calculated from the following Colby's formula. In cases where an incidence rate of a tested composition is lower than the theoretical one, the tested composition can produce a synergistic effect. In these cases, the theoretical incidence rate is shown in parentheses in Tables 21 to 23.

Theoretical incidence rate (X 5 x Y 5 )/100 wherein X 5 is an incidence rate of a plant treated with only Compound No. 1; and Y 5 is an incidence rate of a plant treated with only Cymoxanil, Metalaxyl or Dimethomorph.

Table 21 Curative Effect on Tomato Late Blight (Incidence Rate; Compound Cymoxanil No. 1 2 ppm 0.5 ppm 0 ppm 500 ppm 0 10) 4 10) 125 ppm 70 85) 60 85) 31 ppm 70 (100) 85 (100) 100 8 ppm 85 (100) 100 100 0 ppm 100 100 100 Table 22 Curative Effect on Tomato Late Blight (Incidence Rate: Compound Metalaxyl No. 1 8 ppm 2 ppm 0.5 ppm 0 ppm 500 ppm 2 2 0 7) 125 ppm 0 (13) 2 (13) 10 (59) 31 ppm 7 (15) 7 (15) 20 (70) 100 8 ppm 7 (15) 10 (15) 60 (70) 100 0 ppm 15 15 70 100 Table 23 Curative Effect on Tomato Late Blight (Incidence Rate; Compound Dimethomorph No. 1 31 ppm 8 ppm 0 ppm 500 ppm 0 8.5) 0 10) 125 ppm 4 (72) 20 85) 31 ppm 60 (85) 70 (100) 100 8 ppm 30 (85) 100 100 0 ppm 85 100 100 Test Example 7 Field Test on Cucumber Downy Mildew Seven cucumber seedlings (cultivar: Tokiwa Kohai Hikari No. 3, P type) were planted in a divided area m 2 each) of the field located in Kusatsu City, Shiga, Japan on May 9, 1995. A composition containing Compound No. 1 and Chlorothalonil in the respective concentrations shown in Table 24 below was sprayed over the plants in an amount of 500 to 750 ml per area by means of a small-sized spraying machine on May 30 and June 6. On June 14, all the leaves were observed to obtain a control index in accordance with the following rating system. The results obtained are shown in Table 24. Artificial infection with a pathogenic fungus was not conducted so that the disease was spontaneous.

Control Index Severity of Disease The lesion area or length is less than 7% of that of a control (non-treated area).

The lesion area or length is 7% or more and less than 10% of that of the control.

The lesion area or length is 10% or more and less than 20% of that of the control.

2 The lesion area or length is 20% or more and less than 30% of that of the control.

1 The lesion area or length is 30% or more of that of the control.

Table 24 Field Test on Cucumber Downy Mildew (Control Index) Chlorothalonil Compound No. 1 400 ppm 0 ppm 12.5 ppm 5 3 0 ppm 1 1 Test Example 8 Test of Preventive Effect on Cucumber Downv Mildew A cucumber (cultivar: polyethylene pots (diameter: reached a two-leaf stage, containing Compound No. 1 and Suyo) was cultivated in 7.5 cm). When the plant 10 ml of a composition Doitsu Borudo A (trade name of copper oxychloride wettable powder produced by Hokko Chemical Industry Co., Ltd.) in respective concentrations shown in Table 25 below was sprayed on the seedling by means of a spray gun. After 24 hours, it was inoculated by spraying a spore suspension of fungi of downy mildew (Pseudopernospora cubensis). The plant was kept in a chamber set at 22 to 24°C for 6 days, and the lesion area of the first leaf was measured, from which the disease incidence rate was calculated according to the following formula. The results obtained are shown in Table Incidence rate x 100 wherein a is a lesion area of a treated plant; and b is a lesion area of a control (non-treated plant).

A theoretical incidence rate can be calculated from the following Colby's formula. In cases where an incidence rate of a tested composition is lower than the theoretical one, the tested composition can be the to produce a synergistic effect. In these cases, the theoretical incidence rate is shown in parentheses in Table Theoretical incidence rate (X 6 x y 6 )/100 wherein X 6 is an incidence rate of a plant treated with only Compound No. 1; and Y6 is an incidence rate of a plant treated with only Doitsu Borudo A.

Table Preventive Effect on Cucumber Downy Mildew (Incidence Rate; Doitsu Borudo A Compound No. 1 ppm 0 ppm 0.

2 ppm 0 7) 7 0.025 ppm 70 (100) 100 0 ppm 100 100 Test Example 9 Test of Preventive Effect on Tomato Late Blight A tomato (cultivar: Ponderosa) was cultivated in polyethylene pots (diameter: 7.5 cm). When the plant reached a four-leaf stage, 10 ml of a composition containing Compound No. 1 and Kocide Bordeaux (trade name of a cupric hydroxide wettable powder produced by Griffin) or Doitsu Borudo A (trade name of copper oxychloride wettable powder produced by Hokko Chemical Industry Co., Ltd.) in the respective concentrations shown in Tables 26 and 27 below was sprayed on the seedling by means of a spray gun. After 24 hours, it was inoculated by spraying a zoosporangium suspension of fungi of late blight (Phytophthora infestans) The plant was kept in a chamber set at 22 to 24°C for 3 days, and the lesion area was measured, from which the disease incidence rate was calculated in the same manner as in Test Example 1. The results obtained are shown in Tables 26 and 27.

A theoretical incidence rate can be calculated from the following Colby's formula. In cases where an incidence rate of a tested composition is lower than the theoretical one, the tested composition can be the to produce a synergistic effect. In these cases, the theoretical incidence rate is shown in parentheses in Tables 26 and 27.

Theoretical incidence rate (X 7 x Y)/100 wherein X is an incidence rate of a plant treated with only Compound No. 1; and Y 7 is an incidence rate of a plant treated with only Kocide Bordeaux or Doitsu Borudo A.

Table 26 Preventive Effect on Tomato Late Blight (Incidence Rate: Kocide Bordeaux Compound No. 1 ppm 0 ppm 0.8 ppm 9 (47) 47 0.4 ppm 37 (50) 0 ppm 100 100 Table 27 Preventive Effect on Tomato Late Blight (Incidence Rate; Doitsu Borudo A Compound No. 1 200 ppm 0 ppm 3 ppm 0 (31) 31 ppm 3 (37) 37 0 ppm 100 100 Test Example Test of Curative Effect on Cucumber Downy Mildew A cucumber (cultivar: Suyo) was cultivated in polyethylene pots (diameter: 7.5 cm). When the plant reached a two-leaf stage, it was inoculated by spraying a spore suspension of fungi of downy mildew (Pseudoperonospora cubensis) After 24 hours, 10 ml of a composition containing Compound No. 1 and aluminium tris (ethyl phosphonate) (Fosetyl-aluminium) in the respective concentrations shown in Table 28 was sprayed onto the plant by means of a spray gun. The plant was kept in a chamber set at 22 to 24 0 C for 6 days, and the lesion area of the first leaf was measured, from which the disease incidence rate was calculated in the same manner as in Test Example 1. The results obtained are shown in Table 28.

A theoretical incidence rate can be calculated from the following Colby's formula. In cases where an incidence rate of a tested composition is lower than the theoretical one, the tested composition can produce a synergistic effect. In these cases, the theoretical incidence rate is shown in parentheses in Table 28.

Theoretical incidence rate (X 8 x Y )/100 wherein X 8 is an incidence rate of a plant treated with only Compound No. 1; and Y 8 is an incidence rate of a plant treated with only Fosetyl-aluminium Table 28 Curative Effect on Cucumber Downy Mildew (Incidence Rate; Compound Fosetyl-Aluminum No. 1 2000 ppm 500 ppm 0 ppm ppm 12.5 (48.8) 40 (55.3) 0 ppm 75 85 100 Test Example 11 Test of Curative Effect on Cucumber Downy Mildew Preparation of Aqueous Dispersion: A spreader (activity-enhancing ingredient) shown in Table 29 below was 500-fold or 1000-fold diluted with water, and Compound No. 1 was added thereto in a concentration of 100 ppm or 12.5 ppm to prepare an aqueous dispersion. For comparison, an aqueous dispersion containing 100 ppm or 12.5 ppm of Compound No.

1 and containing no activity-enhancing ingredient was prepared in the same manner.

Test Method and Results: A cucumber (cultivar: Suyo) was cultivated in polyethylene pots (diameter: 7.5 cm) When the plant reached a two-leaf stage, it was inoculated by spraying a spore suspension of fungi of downy mildew (Pseudoperonospora cubensis). After 15 to 24 hours, the aqueous dispersion was sprayed over the plant with a spray gun in an amount of 20 ml per 0.25 m 2 The plant was kept in a chamber set at 22 to 24 0 C for 4 to 6 days, and the lesion area of the first leaf was measured to obtain a control index in accordance with the following rating system. The results obtained are shown in Table 29.

Severity of Disease The lesion area or length is less than 20% of that of a control (non-treated plant).

The lesion area or length is 20% or more and less than 40% of that of the control.

The lesion area or length is 40% or more and less than 60% of that of the control.

The lesion area or length is 60% or more of that of the control.

Table 29 Control Control Activity- Index Activity- Index enhancing enhancing ingredient Conc. of ingredient Cone. of (Spreader) Compound (Spreader) Compound No. 1 (ppm) No. 1 (ppm) No. Dilution 100 12.5 No. Dilution 100 12.5 Rate Rate 1 500-fold 4 23 500-fold 4 2 500-fold 4 25 500-fold 4 3 500-fold 4 26 500-fold 4 4 4 500-fold 4 3 27 500-fold 4 4 500-fold 4 3 28 500-fold 4 6 500-fold 4 2 29 500-fold 4 7 500-fold 4 4 30 500-fold 4 8 500-fold 4 4 31 500-fold 4 9 500-fold 4 32 500-fold 4 1000-fold 4 3 33 500-fold 4 11 500-fold 4 34 500-fold 4 12 500-fold 4 35 500-fold 4 13 500-fold 4 36 500-fold 4 3 14 500-fold 4 37 500-fold 4 4 500-fold 4 38 500-fold 4 4 16 500-fold 4 40 500-fold 4 17 500-fold 4 41 500-fold 4 18 500-fold 4 42 500-fold 4 19 500-fold 4 43 500-fold 4 500-fold 4 44 500-fold 4 21 500-fold 3 45 500-fold 4 3 22 500-fold 4 46 500-fold 4 /To be cont'd.

Table 29 (cont'd.) Control Control Activity- Index Activity- Index enhancing enhancing ingredient Conc. of ingredient Cone. of (Spreader) Compound (Spreader) Compound No. 1 (ppm) No. 1 (ppm) [Dilution Dilution No. Dilution 100 12.5 No. Dilut 100 12.5 Rate Rate 47 500-fold 4 68 1000-fold 4 48 500-fold 4 4 70 500-fold 4 49 500-fold 4 3 71 500-fold 4 500-fold 4 4 72 500-fold 4 4 51 500-fold 4 4 73 500-fold 3 52 500-fold 4 4 74 500-fold 4 53 500-fold 4 4 75 500-fold 4 54 500-fold 4 4 76 500-fold 4 4 500-fold 4 4 77 500-fold 4 4 56 500-fold 4 78 500-fold 4 57 500-fold 4 4 79 500-fold 4 59 500-fold 4 4 8O 500-fold 4 500-fold 4 4 81 500-fold 4 61 500-fold 4 82 500-fold 4 62 500-fold 4 83 500-fold 4 63 500-fold 4 84 500-fold 4 64 500-fold 4 85 500-fold 4 500-fold 4 86 500-fold 4 66 500-fold 4 87 500-fold 4 67 500-fold 4 none 1 1 Test Example 12 Test of Curative Effect on Tomato Late Blight Preparation of Aqueous Dispersion: A spreader (activity-enhancing ingredient) shown in Table 30 below was 500-fold diluted with water, and Compound No. 1 was added thereto in a concentration of 400 ppm or 12.5 ppm to prepare an aqueous dispersion.

For comparison, an aqueous dispersion was prepared in the same manner, except for using a sorbitan fatty acid ester surface active agent shown in Table below (comparative spreader A, B or C) as a spreader and adding Compound No. 1 in a concentration of 400 ppm.

For further comparison, an aqueous dispersion containing 400 ppm or 12.5 ppm of Compound No. 1 and containing no activity-enhancing ingredient was prepared in the same manner.

Table Compara- Trade Name tive Kind Designation (Manufacturer) Spreader polyoxyethylene polyoxyethylene APPLAUCH® (Kao A hexitan fatty hexitan fatty Corporation) acid ester acid ester polyoxyethylene hexitan fatty acid ester polyoxyethylene hexitan fatty acid ester 50% Alsoap (Sankei Chemical Co., Ltd., Takeda Chemical Industries, Ltd.) 1 polyoxyethylene sorbitan fatty acid ester oxysorbic polyoxyethylene sorbitan monolaurate Tween 20 (Wako Pure Chemical Industries, Ltd.) Test Method and Results: A tomato (cultivar: Ponderosa) was cultivated in polyethylene pots (diameter: 7.5 cm) When the plant reached a four-leaf stage, it was inoculated by spraying a zoosporangium suspension of fungi of late blight (Phytophthora infestans) After 4 hours, the aqueous dispersion above prepared was sprayed over the plant with a spray gun in an amount of 20 ml per 0.25 m 2 After the plant was kept in a chamber set at 22 to 24°C for 3 days, the lesion area was measured, from which a control index was obtained in accordance with the same rating system as in Test Example 11. The resull obtained are shown in Table 31.

Table 31 Curative Effect on Tomato Late Blioht (Control Index) ts Control Control Activity- Index Activity- Index enhancing Cone. of enhancing Cone. of ingredient Compound ingredient Compound (Spreader) No. 1 (Spreader) No. 1 (ppm) (ppm) Dilution Dilution No. 400 12.5 No. n 400 12.5 Rate Rate 7 500-fold 3 55 500-fold 3 19 500-fold 4 61 500-fold 3 22 500-fold 3 67 500-fold 4 23 500-fold 4 76 500-fold 3 500-fold 3 88 500-fold 3 27 500-fold 3 90 500-fold 4 500-fold 4 Comparative 39 500-fold 4 A 500-fold 2 42 500-fold 4 B 500-fold 2 43 500-fold 4 C 500-fold 2 46 500-fold 3 none 1 1 Test Example 13 Test of Curative Effect on Tomato Late Blight Preparation of Aqueous Dispersion: A spreader (activity-enhancing ingredient) shown in Table 32 below was 2000-fold diluted with water, and Compound No. 1 was added thereto in a concentration of 100 ppm to prepare an aqueous dispersion. For comparison, an aqueous dispersion containing 100 ppm of Compound No. 1 and containing no activity-enhancing ingredient was prepared in the same manner.

Test Method and Results: A tomato (cultivar: Ponderosa) was cultivated in polyethylene pots (diameter: 7.5 cm) When the plant reached a four-leaf stage, it was inoculated by spraying a zoosporangium suspension of fungi of late blight (Phytophthora infestans) After 4 hours, the aqueous dispersion above prepared was sprayed over the plant with a spray gun in an amount of 20 ml per 0.25 m 2 After the plant was kept in a chamber set at 22 to 24°C for 3 days, the lesion area was measured, from which a control index was obtained in accordance with the same rating system as in Test Example 11. The results obtained are shown in Table 32.

Table 32 Curative Effect on Tomato Late Blight (Control Index) Activity-enhancing ingredient No Activityenhancing Spreader No. 23 24 39 88 90 ng in ingredient Control Index 4 4 4 4 4 2 Test Example 14 Test of Preventive Effect on Tomato Late Blight Preparation of Aqueous Dispersion: Spreader No. 58 or 91 (activity-enhancing ingredient) was 500-fold diluted with water, and Compound No. 1 was added thereto in a prescribed concentration to prepare an aqueous dispersion. For comparison, an aqueous dispersion containing the same concentration of Compound No. 1 and containing no activity-enhancing ingredient was prepared in the same manner.

Test Method and Results: A tomato (cultivar: Ponderosa) was cultivated in polyethylene pots (diameter: 7.5 cm). When the plant reached a four-leaf stage, the aqueous dispersion above prepared was sprayed over the plant with a spray gun in an amount of 20 ml per 0.25 m 2 After 24 hours from the spray treatment, a zoosporangium suspension of fungi of late blight (Phytophthora infestans) was sprayed for inoculation, and the plant was kept in a chamber set at 22 to 24°C for 3 days. The lesion area was measured, from which a control index was obtained in accordance with the same rating system as in Test Example 11. The results obtained are shown in Table 33.

Table 33 Preventive Effect on Tomato Late Blight (Control Index) Activity-enhancing ingredient Compound Spreader No. Spreader No.

No. 1 58 (500-fold 91 (500-fold None diluted) diluted) 1.6 ppm 4 4 3 0.8 ppm 4 4 1 0.4 ppm 1 3 1 Test Example Test of Curative Effect on Cucumber Downy Mildew Preparation of Aqueous Dispersion: A spreader (activity-enhancing ingredient) shown in Table 34 below was 500-fold or 2000-fold diluted with water, and Compound No. 1 was added thereto in a concentration of 12.5 ppm to prepare an aqueous dispersion. For comparison, an aqueous dispersion containing 12.5 ppm of Compound No. 1 and containing no activity-enhancing ingredient was prepared in the same manner.

Test Method and Results: A cucumber (cultivar: Suyo) was cultivated in polyethylene pots (diameter: 7.5 cm). When the plant reached a two-leaf stage, it was inoculated by spraying a spore suspension of fungi of downy mildew (Pseudoperonospora cubensis) After 15 to 24 hours, the aqueous dispersion was sprayed over the plant with a spray gun in an amount of 20 ml per 0.25 m 2 The plant was kept in a chamber set at 22 to 24 0 C for 5 days, and the lesion area of the first leaf was measured to obtain the lesion-free area ratio The results obtained are shown in Table 34.

Table 34 Curative Effect on Cucumber Downy Mildew (Lesion-free Area Ratio: Dilution Ratio Spreader No.

of Activity- 23 24 39 88 89 90 none enhancing ingredient 500-fold 100 100 100 95 90 100 2000-ld 100 100 100 83 2000-fold 100 100 100 83 (note: all parts on the following Formulation Examples 1-14 and Reference Formulation Example are indicated by weight.) Formulation Example 1 Compound No. 1 5 parts (by weight, hereinafter the same) Dipotassium hydrogenphosphate 7 parts Diatomaceous earth 82 parts Dialkyl sulfosuccinate 2 parts Polyoxyethylene alkylphenyl ether sulfate 4 parts The above components were mixed uniformly to obtain a wettable powder.

Formulation Example 2 Compound No. 1 5 parts Sodium tertiary phosphate dodecahydrate 16 parts Diatomaceous earth 73 parts Dialkyl sulfosuccinate 2 parts Polyoxyethylene alkylphenyl ether sulfate 4 parts The above components were mixed uniformly to obtain a wettable powder.

Formulation Example 3 Compound No. 1 5 parts Dipotassium hydrogenphosphate 18 parts Kerosene 63 parts Dialkyl sulfosuccinate 2 parts Mixture of polyoxyethylene phenylphenol 12 parts derivative and polyoxyethylene sorbitan alkylate The above components were mixed uniformly and finely ground to obtain a suspension concentrate.

Formulation Example 4 Kaolin 78 parts Sodium p-naphthalenesulfonate-formaldehyde 2 parts condensate Polyoxyethylene alkylaryl sulfate 5 parts Hydrated amorphous silicon dioxide 15 parts A mixture of the above components, dipotassium hydrogenphosphate, and Compound No. 1 were mixed at a weight ratio of 79:20:1 to obtain a wettable powder.

Formulation Example Kaolin 78 parts Sodium p-naphthalenesulfonate-formaldehyde 2 parts condensate Polyoxyethylene alkylaryl sulfate 5 parts Hydrated amorphous silicon dioxide 15 parts A mixture of the above components, Compound No. 1, and Metalaxyl were mixed at a weight ratio of 8:1:1 to obtain a wettable powder.

Formulation Example 6 Compound No. 2 0.5 part Metalaxyl 0.5 part Bentonite 20 parts Kaolin 74 parts Sodium lignin sulfonate 5 parts The above components were mixed together with an adequate amount of water enough for granulation, followed by granulation to obtain granules.

Formulation Example 7 Compound No. 3 0.25 part Metalaxyl 0.25 part Calcium carbonate 99.0 parts Lower alcohol phosphate 0.5 part The above components were mixed uniformly to obtain a dust.

Formulation Example 8 Kaolin 78 parts Sodium p-naphthalenesulfonate-formaldehyde 2 parts condensate Polyoxyethylene alkylaryl sulfate 5 parts Hydrated amorphous silicon dioxide 15 parts A mixture of the above components, Compound No. 1, and Kocide Bordeaux (trade name) were mixed at a weight ratio of 0.8:76.8:22.4 to obtain a wettable powder.

Formulation Example 9 Kaolin 78 parts Sodium p-naphthalenesulfonate-formaldehyde 2 parts condensate Polyoxyethylene alkylaryl sulfate 5 parts Hydrated amorphous silicon dioxide 15 parts A mixture of the above components, Compound No. 1, and Duitch Bordeaux A (trade name) were mixed at weight ratio of 5:67.2:27.8 to obtain a wettable powder.

Formulation Example Compound No. 1 0.25 part Sanpun Bordeaux Dust DL (trade name, 0.25 part produced by Dai-ichi Noyaku K.K. and Hokko Chemical Industry Co., Ltd.) Sodium carbonate 99.0 parts Lower alcohol phosphate 0.5 part The above components were mixed uniformly to obtain a dust.

Formulation Example 11 Compound No. 1 Sanpun Bordeaux Dust DL (trade name, produced by Dai-ichi Noyaku K.K. and Hokko Chemical Industry Co., Ltd.) Bentonite Kaolin Sodium lignin sulfonate 0.5 part 0.5 part 20 parts 74 parts 5 parts The above components were mixed together with an adequate amount of water enough for granulation, followed by granulation to obtain granules.

Formulation Example 12 Compound No. 1 5 parts Aluminum tris(ethyl phosphonate) 5 parts (Fosetyl-aluminum) Diatomaceous earth 84 parts Calcium lignin sulfonate 2 parts Dialkyl sulfosuccinate 4 parts The above components were mixed uniformly to obtain a wettable powder.

Formulation Example 13 Compound No. 1 (active ingredient) 11.1 parts Dispersant SOPROPHOR FLK (trade name, 1.1 part produced by RH6NE-POULENC) Dispersing and wetting agent Supragil 1.1 part (trade name) Dispersing and suspending agent Vegum 1.7 parts Urea (acting as an antifreezing agent) 11.1 parts Antifoaming agent SM5572F (trade name) 0.1 part Distilled water 73.8 parts The above components to were mixed and wet ground until the active ingredient had an average particle size of 2 pm to prepare a suspension. To parts of the resulting suspension was added 10 parts of an activity-enhancing ingredient, followed by mixing by shaking to prepare an aqueous suspension concentrate.

Formulation Example 14 Compound No. 1 (active ingredient) 10.0 parts Dispersant SOPROPHOR FLK (trade name, 1.0 part produced by RHONE-POULENC) Dispersing and wetting agent Supragil 1.0 part (trade name) Dispersing and suspending agent Vegum 1.5 parts Urea (acting as an antifreezing agent) 10.0 parts Antifoaming agent SM5572F (trade name) 0.1 part Distilled water 66.4 parts Activity-enhancing ingredient 10.0 parts The above components to were mixed and wet ground until the active ingredient had an average particle size of 2 pm to prepare an aqueous suspension concentrate.

Reference Formulation Example Kaolin 78 parts Sodium p-naphthalenesulfonate-formaldehyde 2 parts condensate Polyoxyethylene alkylaryl sulfate 5 parts Hydrated amorphous silicon dioxide 15 parts The above components and an inorganic phosphorous compound were mixed at a weight ratio of 4:1 to prepare a 20% wettable powder of the inorganic phosphorous compound.

Industrial Applicability The compositions for controlling harmful bioorganisms according to the present invention have high curative and/or -preventive effects on crop plants suffering from plant diseases caused by harmful bioorganisms and can control the harmful bio-organisms.

The compositions containing the activity-enhancing.

ingredient exhibit enhanced curative effects so that the amount of the active ingredient can be reduced.

While the invention has been described in detail and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that various changes and modifications can be made therein without departing from the spirit and scope thereof.

A reference herein to a prior art document is *not an admission that the document forms part of the common general knowledge in the art in Australia.

In the claims which follow and in the preceding description of the invention, except where the context requires otherwise due to express language or necessary implication, the word "comprise" or variations such as "comprises" or "comprising" is used in an inclusive sense, i.e. to specify the presence of the stated features but not topreclude the presence or addition of further features in various embodiments of the invention.

Claims (9)

1. A composition for controlling harmful bio-organisms comprising at least one imidazole compound represented by formula Cl N kn (I) SO 2 N(CH 3 2 wherein R represents a lower alkyl group or a lower alkoxy group; and n represents an integer of 1 to as an active ingredient, and at least one inorganic phosphorus compound and/or at least one fungicide for Phycomycetes which is at least one member selected from the group consisting of a P-methoxyacrylate compound, an oxazolidinedione compound, a phenylamide compound, a cinnamic acid compound and a copper compound as an active ingredient.

2. The composition according to claim 1, wherein the active ingredient is at least one inorganic phosphorous compound.

3. The composition according to claim 2, wherein the inorganic phosphorous compound is a compound selected fromthe group consisting of phosphoric acid, phosphorous acid, hypophosphorous acid, a condensed phosphoric acid, and a condensed phosphorous acid, and salts thereof.

4. The composition according to claim 2, wherein the imidazole compound and the inorganic phosphorous compound are at a weight ratio of 1:300 to 300:1. The composition according to claim 1, wherein the active ingredient is at least one fungicide for Phycomycetes which is at least one member selected from the group consisting of p-methoxyacrylate compound, an oxazolidinedione compound, a phenylamide compound, a cinnamic acid compound and a copper compound.

6. The composition according to claim 5, wherein the fungicide is a compound selected from the group consisting of: methyl (E)-2-{2-[6-(2-cyanophenoxy)pyrimidin-4- yloxy]phenyl}-3-methoxyacrylate, methyl (E)-methoxyimino[a-(o-tolyloxy)-0- tolyl]acetate, 3-anilino-5-methyl-5-(4-phenoxyphenyl)-1,3- oxazolidine-2,4-dione, methyl N-(2-methoxyacetyl)-N-(2,6-xylyl)-DL- alaninate, 2-methoxy-N-(2-oxo-l,3-oxazolidin-3-yl)aceto- 2',6'-xylidide, (±)-a-2-chloro-N-(2,6-xylylacetamide)-y- butyrolactone, methyl N-phenylacetyl-N-(2,6-xylyl)-DL-alaninate, methyl N-(2-furoyl)-N-(2,6-xylyl)-DL-alaninate, (±)-a-[N-(3-chlorophenyl)cyclopropane- carboxamide]-y-butyrolactone, (E,Z)-4-[3-(4-chlorophenyl)-3-(3,4- dimethoxyphenyl)acryloyl]morpholine, an inorganic copper fungicide and an organic copper fungicide.

7. The composition according to claim 5, wherein the fungicide is a compound selected from the group consisting of: methyl (E)-2-{2-[6-(2-cyanophenoxy)pyrimidine-4- yloxy]phenyl}-3-methoxyacrylate, methyl (E)-methoxyimino[a-(o-tolyloxy)-0- tolyl]acetate, 3-anilino-5-methyl-5-(4-phenoxyphenyl)-1,3- oxazolidine-2,4-dione, methyl N-(2-methoxyacetyl)-N-(2,6-xylyl)-DL- alaninate, (E,Z)-4-[3-(4-chlorophenyl)-3-(3,4- dimethoxyphenyl)acryloyl]morpholine and an inorganic copper fungicide.

8. The composition according to claim 5, wherein the imidazole compound and the fungicide are at a weight ratio of 1:10000 to 10000:1.

9. A method for controlling harmful bio-organisms comprising applying a composition for controlling harmful bio-organisms onto harmful bio-organisms, wherein the composition comprises: 100 at least one imidazole compound represented by formula N SO 2 N(CH 3 2 wherein R represents a lower alkyl group or a lower alkoxy group; and n represents an integer of 1 to as an active ingredient, and at least one inorganic phosphorus compound and/or at least one fungicide for Phycomycetes which is at least one member selected from the group consisting of a P-methoxyacrylate compound, an oxazolidinedione compound, a phenylamide compound, a cinnamic acid compound and a copper compound as an active ingredient. A composition according to claim 1 substantially as herein described with reference to any one of Test Examples 1 to 6, 8, 9 or Formulation Examples 1 to 11.

11. A method according to claim 9 substantially as herein described with reference to any one of Test Examples 1 to 6, 8 and 9. Dated this 16th day of July 2004 ISHIHARA SANGYO KAISHA, LTD. By its Patent Attorneys GRIFFITH HACK 101