AU2002239369A1 - Eukaryotic expression libraries based on double lox recombination and methods of use - Google Patents

Eukaryotic expression libraries based on double lox recombination and methods of use

Info

Publication number
AU2002239369A1
AU2002239369A1 AU2002239369A AU3936902A AU2002239369A1 AU 2002239369 A1 AU2002239369 A1 AU 2002239369A1 AU 2002239369 A AU2002239369 A AU 2002239369A AU 3936902 A AU3936902 A AU 3936902A AU 2002239369 A1 AU2002239369 A1 AU 2002239369A1
Authority
AU
Australia
Prior art keywords
methods
eukaryotic expression
expression libraries
libraries based
lox recombination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002239369A
Inventor
William D. Huse
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Molecular Evolution Inc
Original Assignee
Applied Molecular Evolution Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Molecular Evolution Inc filed Critical Applied Molecular Evolution Inc
Publication of AU2002239369A1 publication Critical patent/AU2002239369A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
AU2002239369A 2000-11-28 2001-11-28 Eukaryotic expression libraries based on double lox recombination and methods of use Abandoned AU2002239369A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US72476200A 2000-11-28 2000-11-28
US09/724,762 2000-11-28
PCT/US2001/044600 WO2002044361A2 (en) 2000-11-28 2001-11-28 Eukaryotic expression libraries based on double lox recombination and methods of use

Publications (1)

Publication Number Publication Date
AU2002239369A1 true AU2002239369A1 (en) 2002-06-11

Family

ID=24911795

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002239369A Abandoned AU2002239369A1 (en) 2000-11-28 2001-11-28 Eukaryotic expression libraries based on double lox recombination and methods of use

Country Status (6)

Country Link
US (1) US20040087014A1 (en)
EP (1) EP1337631A2 (en)
JP (1) JP2004514444A (en)
AU (1) AU2002239369A1 (en)
CA (1) CA2430080A1 (en)
WO (1) WO2002044361A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7070973B2 (en) * 2000-12-26 2006-07-04 Board Of Regents Of The University Of Nebraska Butyrylcholinesterase variants and methods of use
AU2004203727C1 (en) * 2003-01-07 2008-08-21 Symphogen A/S Method for manufacturing recombinant polyclonal proteins
CN100383244C (en) * 2003-01-07 2008-04-23 西福根有限公司 Method for manufacturing recombinant polyclonal proteins
MX2007000644A (en) 2004-07-20 2007-03-28 Symphogen As Anti-rhesus d recombinant polyclonal antibody and methods of manufacture.
AU2005263334C1 (en) * 2004-07-20 2011-01-20 Symphogen A/S A procedure for structural characterization of a recombinant polyclonal protein or a polyclonal cell line
KR20110016899A (en) * 2008-04-23 2011-02-18 심포젠 에이/에스 Methods for manufacturing a polyclonal protein
CN102711795A (en) 2009-12-08 2012-10-03 泰华制药工业有限公司 BChE albumin fusions for the treatment of cocaine abuse
EP2882854B1 (en) * 2012-08-10 2018-10-31 The Broad Institute, Inc. Methods for generating pools of variants of a dna template
AR094481A1 (en) 2013-01-15 2015-08-05 Teva Pharma FORMULATIONS OF ALBU-BCHE (ALBUMINA-BUTIRILCOLINESTERASA), PREPARATION AND ITS USES
GB201407852D0 (en) 2014-05-02 2014-06-18 Iontas Ltd Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules
CN109563508B (en) 2016-07-29 2023-07-07 马克思—普朗克科学促进协会公司 Targeting in situ protein diversification by site-directed DNA cleavage and repair
US20200385710A1 (en) * 2018-02-08 2020-12-10 Applied Stemcell, Inc. Methods for screening variant of target gene

Also Published As

Publication number Publication date
CA2430080A1 (en) 2002-06-06
US20040087014A1 (en) 2004-05-06
JP2004514444A (en) 2004-05-20
WO2002044361A8 (en) 2003-10-30
WO2002044361A2 (en) 2002-06-06
EP1337631A2 (en) 2003-08-27
WO2002044361A3 (en) 2002-08-22

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