AU2002233354A1

AU2002233354A1 - PEG-conjugates of HGT-NK4

Info

Publication number: AU2002233354A1
Application number: AU2002233354A
Authority: AU
Inventors: Michael Brandt; Apollon Papadimitriou
Original assignee: F Hoffmann La Roche AG
Current assignee: F Hoffmann La Roche AG
Priority date: 2001-02-23
Filing date: 2002-02-21
Publication date: 2003-03-27
Anticipated expiration: 2022-02-21

Description

PEG-conjugates of HGF-NK4

This invention relates to conjugates of the N-terminal four kringle-containing fragment of hepatocyte growth factor (NK4) with polyethylene glycol (PEG), pharmaceutical compositions thereof, methods for the production and methods for use.

Background of the Invention

Hepatocyte growth factor (HGF/SF) is a polypeptide identified and purified by Nakamura, T., et al., Biochem. Biophys. Res. Commun. 22 (1984) 1450-1459. It was further found that hepatocyte growth factor is identical to scatter factor (SF), Weidner, K.M., et al., Proc. Natl. Acad. Sci. USA 88 (1991) 7001-7005. HGF is a glycoprotein with a molecular weight of about 100 kDa involved in the development of a number of cellular phenotypes including proliferation, mitogenesis, formation of branching tubules and, in the case of tumor cells, invasion and metastasis. For a status review, see Stuart, K.A., et al., International Journal of Experimental Pathology 81 (2000) 17-30. Both rat HGF and human HGF have been sequenced and cloned (Miyazawa, K. et al., Biochem. Biophys. Res.

Comm. 163 (1989) 967-973; Nakamura, T., et al., Nature 342 (1989) 440-443; Seki, T., et al., Biochem. and Biophys. Res. Comm. 172 (1990) 321-327; Tashiro, K., et al., Proc. Natl. Acad. Sci. USA 87 (1990) 3200-3204; Okajima, A., et al., Eur. J. Biochem. 193 (1990) 375-381). The pharmacokinetics and pharmacological effects of an HGF lacking the first five N-terminal amino acids (dHGF) were investigated by Uematsu, Y., et al., J. Pharm. Sciences 88 (1999) 131-135. It was found that the serum concentration of dHGF decreased rapidly and therefore infusion would be preferred against bolus injection as administration route.

U.S. Patent No. 5,977,310 describes PEG-modified HGF. Such PEG-modified HGF has a prolonged clearance in vivo and has the same physiological activity as HGF.

However, according to U.S. Patent No. 5,977,310, it is only possible to prolong the half life of HGF from 59.2 minutes to 76.7 minutes or 95.6 minutes, respectively (see Example 5 of U.S. Patent No. 5,977,310). It is further suggested in this patent that the molar amount of the PEG reagent maybe selected from the range of from 5 to 100 times of the molar weight of HGF. In the case of modifying an amino group of lysine or the N-terminus of protein, a preferred molar range of the PEG reagent is of from 10 to 25 times of the molar weight of HGF. The molecular weight of the attached PEG chain was about 10 kDa. Methods for the synthesis of conjugates consisting of PEG and polypeptides such as HGF are also described in WO 94/13322. These conjugates are linked together at predefined positions as random conjugation leads, according to the authors, to the introduction of polymeric moieties into domains of the molecule that mediate the therapeutically or diagnostically desirable activities. Consequently, the molecules may aquire a prolonged half-life in vivo and, in the case of heterologous proteins, reduced immunogenicity, but at the expense of a significant or complete loss of the desired biological activities (see, e.g., Kitamura, K., et al, Cancer Res. 51 (1991) 4310-4315 and Maiti, P.K., et al., Int. J. Cancer Suppl. 3 (1988) 17-22). PEGylated IFN-α shows, for example, only 7% of the potency compared to non-PEGylated IFN-α (Bailon, P., Bioconjugate Chem. 12 (2001) 195-202).

It was further found that an HGF/SF fragment, termed NK4, consisting of the N-terminal hairpin domain and the four kringle domains of HGF/SF has pharmacological properties that are completely different from those of HGF/SF, and is an antagonist to the influence of HGF/SF on the motility and the invasion of colon cancer cells, and is, in addition, an angiogenesis inhibitor that suppresses tumor growth and metastasis (WO 93/23541; Parr, C, et al., Int. J. Cancer 85 (2000) 563-570; Kuba, K., et al., Cancer Res. 60 (2000) 6737-6743; Date, K., et al.,

FEBS Letters 420 (1997) 1-6; Date, K., et al., Oncogene 17 (1989) 3045-3054; Tomioka, D., et al., Cancer Res. 61 (2001) 7518-7524).

As emerges from Kuba, K., et al., Cancer Res. 60 (2000) 6737-6743, in animal experiments, for detecting an effect of NK4 on lung metastases, NK4 had to be infused continuously over a period of two weeks.

It is known that the attachment of polymers to certain polypeptides may increase the serum half life of such polypeptides. This was found, for example, for PEGylated Interleukin-6 (EP 0 442 724) or Interleukin-2 (WO 90/07938) and erythropoietin (WO 01/02017). However, the attachment of polyethylene glycol and other polymers did not necessarily lead to prolongation of their serum half lives. It is known, for example, that the conjugation of different polyethylene glycols to Interleukin-8, G-CSF and other interleukins results in the production of molecules with impaired properties (Mehvar. R., J. Pharm. Pharm. Sci. 3 (1) (2000) 125-136). Thus, the outcome of a PEGylation of a polypeptide is highly unpredictable. Gaertner, H.F., and Offord, R.E., Bioconjugate Chem. 7 (1996) 38- 44 describes the site-specific attachment of PEG to the amino terminus of proteins. Gaertner et al. state (as already mentioned in WO 94/13222, see above) that PEGylation presents a big problem if the attachment sites cannot be precisely controlled, as this might have important implications for protein stability and function.

Francis, G.E., et al., Int. J. Hematol. 68 (1998) 1-18 present an overview of PEGylation of cytokines and other therapeutic proteins. Francis et al. state that with the majority of methods of PEGylation, substantial reduction of bioactivity has been reported (typically, 20-95%). According to Francis et al., PEGylation of proteins is always based on trial and error and virtually all parameters of such a PEGylation can have a surprising and very profound effect on the functionality of the product. Tsutsumi, Y., et al., Thromb. Haemost. 77 (1997) 168-173 describes the PEGylation of Interleukin-6. According to Tsutsumi et al., about 54% of the lysine amino groups of IL-6 were coupled with PEG with a molecular weight of 5 kDa per PEG group. Tsutsumi et al., in Proc. Natl. Acad. Sci. USA 97 (2000) 8548-8553, describe the chemical modification of an immunotoxin by PEG. As random PEGylation was accompanied by a significant loss of specific cytotoxic activity, Tsutsumi performs a site-specific PEGylation by using an immunotoxin mutant with one or two additional cysteins which are used for PEG coupling. Heinzerling, L., et al, Dermatol. 201 (2000) 154-157 describes the coupling of PEG to Interferon-α with a molecular weight of 5 kDa. Tsutsumi, Y., et al., in J. Pharmacol. Exp. Ther. 278 (1996) 1006-1011, describe the PEG modification of TNF-α, whereby the molecular weight of the PEG groups used is again 5 kDa. As the PEGylated TNF-α applied has a molecular weight of at least 84 kDa (by a molecular weight of 17 kDa of TNF-α) there are at least 13 5-kDa PEG groups attached to TNF-α.

PEGylation of proteins and its pharmacological effects are also reviewed by Reddy, K.R., Ann. Pharmacotherapy 34 (2000) 915-923. Again it is stated that PEGylation of therapeutic proteins must be carefully evaluated. Each protein is, according to Reddy et al., different, requires different optimization chemistry and therefore the influence of PEGylation cannot be predicted. It was an object of the present invention to find improved polypeptides with NK4 activity and a pharmaceutical composition thereof which can be administered as only a few bolus applications per week and/or in very low dosages and which are capable of suppressing tumor growth, angiogenesis and metastasis.

Summary of the Invention

The present invention provides NK4 conjugates consisting of NK4 being covalently linked to one polyethylene glycol (PEG) group of about 20-40 kDa (monoPEGylated NK4), preferably via an ε-amino group of NK4 lysine or the N-terminal amino group. Most preferably, NK4 is randomly PEGylated at one amino group out of the group consisting of the ε-amino groups of NK4 lysine and the N-terminal amino group. Surprisingly, it has been found that monoPEGylated NK4 according to the invention has superior properties in regard to the therapeutic applicability in regard to NK4 or otherwise PEGylated NK4.

The invention further comprises a method for the production of monoPEGylated NK4 according to the invention.

The invention further comprises pharmaceutical compositions containing monoPEGylated NK4.

The invention further comprises methods for the production of pharmaceutical compositions containing monoPEGylated NK4.

The invention further comprises methods for the treatment of human cancer (e.g. breast, lung, prostate, pancreas or colon cancer) characterized in that a pharmaceutically effective amount of monoPEGylated NK4 is administered in one to seven bolus applications per week to the patient in need thereof.

It was surprisingly found that the entire amount of randomly PEGylated NK4 according to the invention to be applied during treatment is considerably lower compared to applying unPEGylated NK4. Therefore, the amount of PEGylated NK4 used in pharmaceutical treatment is about 50% or lower, preferably about 20% or lower, and most preferably about 10% or lower of the required amount of unPEGylated NK4. Detailed Description of the Invention

Human HGF is a disulfide-linked heterodimer, which can be cleaved in an α- subunit of 463 amino acids and a β-subunit of 234 amino acids, by cleavage between amino acids R494 and V495. The N-terminus of the α-chain is preceded by 31 amino acids started with a methionine group. This segment includes a signal sequence of 31 amino acids. The α-chain starts at amino acid 32 and contains four kringle domains. The so-called "hairpin domain" consists of amino acids 70-96. The kringle 1 domain consists of amino acids 128-206. The kringle 2 domain consists of amino acids 211-288, the kringle 3 domain consists of amino acids 305-383, and the kringle 4 domain consists of amino acids 391-469 of the α-chain, approximately.

There exist variations of these sequences, essentially not affecting the biological properties of NK4 (especially not affecting its activities antagonistic to HGF and its antiangiogenic activities), which variations are described, for example, in WO 93/23541. Also the length of NK4 can vary within a few amino acids as long as its biological properties are not affected.

NK4 is composed of the N-terminal 447 amino acids of the HGF/SFα-chain, which includes the above-mentioned hairpin domain and the four kringle domains. It can be produced recombinantly, either by the production of recombinant human HGF/SF and digestion with elastase (Date, K., FEBS Letters 420 (1997) 1-6) or by recombinant expression of an NK4 encoding nucleic acid in appropriate host cells, as described below. NK4 glycoprotein has a molecular weight of about 57 kDa (52 kDa for the polypeptide part alone) and has the in vivo biological activity of causing inhibition of tumor growth, angiogenesis and/or metastasis.

"MonoPEGylated NK4" as used herein therefore means that NK4 has attached covalently one polyethylene glycol group with a molecular weight of 20 to 40 kDa.

The group can be attached, preferably randomly, at different sites of the NK4 molecule, preferably, however, at the most reactive sites, e.g., the lysine side chains and the N-terminal amino group. The monoPEGylated NK4 (which therefore preferably is a mixture of monoPEGylated NK4 molecules, PEGylated at different sites which are the ε-amino groups of NK4 lysine and the N-terminal amino group) is at least 90% of the preparation, and most preferably, the monoPEGylated NK4 is 92%, or more, of the preparation. The monoPEGylated NK4 preparations according to the invention are therefore substantially homogeneous enough to display the advantages of a homogeneous preparation, e.g., in a pharmaceutical application.

"Substantially homogeneous" as used herein means that the only PEG-NK4 conjugate molecules produced, contained or used are those having one PEG group attached. The preparation may contain unreacted (i.e., lacking PEG group) protein.

As ascertained by peptide mapping and N-terminal sequencing, one example below provides for the preparation which is at least 90% monoPEG-NK4 conjugate and at most 2 % unreacted protein.

The PEG polymer molecules used according to the invention have a molecular weight of about 20 to 40 kDa, whereby PEG polymers with about 20, 30 or 40 kDa are preferred (by "molecular weight" as used here there is to be understood the mean molecular weight of the PEG; the term "about" indicates that in said PEG preparations, some molecules will weigh more and some less than the stated molecular weight) .

"PEG or PEG group" according to the invention means a residue containing poly(ethylene glycol) as an essential part. Such a PEG can contain further chemical groups which are necessary for binding reactions; which results from the chemical synthesis of the molecule; or which is a spacer for optimal distance of parts of the molecule. In addition, such a PEG can consist of one or more PEG side-chains which are linked together. PEGs with more than one PEG chain are called multiarmed or branched PEGs. Branched PEGs can be prepared, for example, by the addition of polyethylene oxide to various polyols, including glycerol, pentaerythriol, and sorbitol. For example, a four-armed branched PEG can be prepared from pentaerythriol and ethylene oxide. Branched PEG are described in, for example, EP-A 0473 084 and US Patent No. 5,932,462. Especially preferred are

PEGs with two PEG side-chains (PEG2) linked via the primary amino groups of a lysine (Monfardini, C, et al., Bioconjugate Chem. 6 (1995) 62-69). As PEG polymers with a molecular weight of 20-30 kDa linear PEG molecules are preferred and as PEG polymers with a molecular weight of more than 30 kDa, especially with 40 kDa, branched PEGs are preferred. As PEG 40 kDa a two-armed PEG (PEG2) is particularly preferred. According to the invention a method is provided for the production of a substantially homogeneous monoPEGylated NK4. PEGylation of NK4 can be performed according to the methods of the state of the art, for example by reaction of NK4 with electroph-lically actived PEGs (supplier: Shearwater Corp., USA, www.shearwatercorp.com). Preferred PEG reagents are, e.g.,

N-hydroxysuccinimidyl propionates (PEG-SPA) or butanoates (PEG-SBA) or branched N-hydroxysuccinimides such as mPEG2-NHS (Monfardini, C, et al., Bioconjugate Chem. 6 (1995) 62-69). Such methods result in an NK4 polypeptide which is randomly PEGylated at an ε-amino group of an NK4 lysine or the N- terminal amino group. Not randomly, N-terminally PEGylated NK4 can be produced according to WO 94/01451.

In a preferred embodiment of the invention, said NK4 is covalently linked to one polyethylene glycol) group of the formula

-CO-(CH₂)_x-(OCH₂CH₂)_m-OR

with the -CO (i.e. carbonyl) of the poly(ethylene glycol) group forming an amide bond with one of the amino groups of NK4; R being lower alkyl; x being 2 or 3; m being from about 450 to about 950; and n and m being chosen so that the molecular weight of the conjugate minus the NK4 protein is from about 20 to 40 kDa. As amino group of NK4 the ε-amino group of NK4 lysine is the available (free) amino group.

More specifically, the above conjugates ma be represented by formula (I)

P-NHCO-(CH₂)_x-(OCH₂CH₂)_m-OR (I)

wherein P is the group of an NK4 protein as described herein, (i.e. without the amino group or amino groups which form an amide linkage with the carbonyl shown in formula (I); and wherein R is lower alkyl; x is 2 or 3; m is from about 450 to about 950 and is chosen so that the molecular weight of the conjugate minus the NK4 protein is from about 20 to 40 kDa. As used herein, the given ranges of "m" merely have an orientational meaning. The ranges of "m" are determined in any case, and exactly, by the molecular weight of the PEG group.

In a further preferred embodiment of the invention, said NK4 is covalently linked to one poly(ethylene glycol) group of the formula

wherein y is 1 to 4, preferably 4, n and p together are chosen such that the molecular weight of the conjugate minus the NK4 protein is from about 20 to 40 kDa, preferably 40 kDa, and n and p differ by not more than 25%, preferably by not more than 10%, and most preferably are identical, and R is lower alkyl.

As used herein, "lower alkyl" means a linear or branched alkyl group having from one to six carbon atoms ( -Cs) alkyl). Examples of lower alkyl groups include methyl, ethyl and isopropyl. In accordance with this invention, R is any lower alkyl. Conjugates in which R is methyl are preferred.

The symbol "m" represents the number of ethylene oxide groups (OCH₂CH₂) in the poly(ethylene oxide) group. A single PEG subunit of ethylene oxide has a molecular weight of about 44 daltons. Thus, the molecular weight of the conjugate (excluding the molecular weight of the NK4) depends on the number "m". In the conjugates of this invention "m" is from about 450 to about 950 (corresponding to a molecular weight of about 20 kDa to about 40 kDa). The number m is selected such that the resulting conjugate of this invention has a physiological activity comparable to unmodified NK4, which activity may represent the same as, more than, or a fraction of the corresponding activity of unmodified NK4. A molecular weight of "about" a certain number means that it is within a reasonable range of that number as determined by conventional analytical techniques. The number "m" is selected so that the molecular weight of each poly( ethylene glycol) group covalently linked to the NK4 protein is from about 20 kDa to about 40 kDa.

The compound of formula (I) can be prepared, for example, from a known activated polymeric material:

in which R and m are as described above, by condensing the compound of Formula II with the NK4 protein. Compounds of formula (II) in which x is 3 are alpha-lower alkoxybutyric acid succinimidyl esters of poly( ethylene glycol) (lower alkoxy-PEG- SBA). Compounds of formula (II) in which x is 2 are alpha-lower alkoxypropionic acid succinimidyl esters of poly( ethylene glycol) (lower alkoxy-PEG-SPA). Any conventional method of reacting an activated ester with an amine to form an amide can be utilized. In the reaction described above, the exemplified succinimidyl ester is a leaving group causing the amide formation. The use of succinimidyl esters such as the compounds of formula II to produce conjugates with proteins are disclosed in U.S. Patent No. 5,672,662, issued September 30, 1997 (Harris, et al).

Human NK4 contains 30 free ε-amino groups of 30 lysine residues. When the PEGylation reagent was combined with a SBA compound of Formula II, it has been found that at a protein concentration of about 5 to 10 mg/ml, at a pH of about 7.0 to 8.0, a proteimPEG ratio of about 1:3 and a reaction temperature of from 20- 25°C, a mixture of mono-, di-, and trace amounts of the tri-PEGylated species were produced. When the protein:PEG ratio was about 1:1 or 1:2 (for example, preferably about 1:2 for 30 kDa PEG-SBA and about 1:5 for 40 kDa PEG2-NHS), primarily the monoPEGylated species is produced. By manipulating the reaction conditions (e.g., ratio of reagents, pH, temperature, protein concentration, time of reaction etc.), the relative amounts of the different monoPEGylated species can be optimized. Typical, but not limiting, conditions are about 8 to 12 mg/ml NK4, 0.3 M potassium phosphate, pH 8, 25°C, reaction time of 1 h. Under such conditions using 30 kDa PEG-SBA (1:2, protei PEG), the yield is about 38% monoPEGylated NK4.

Monopegylated NK4 can also be produced according to the methods described in WO 94/01451. WO 94/01451 describes a method for preparing a recombinant polypeptide with a modified terminal amino acid alpha-carbon reactive group. The steps of the method involve forming the recombinant polypeptide and protecting it with one or more biologically added protecting groups at the N-terminal alpha- amine and C-terminal alpha- carboxyl. The polypeptide can then be reacted with chemical protecting agents to selectively protect reactive side chain groups and thereby prevent side chain groups from being modified. The polypeptide is then cleaved with a cleavage reagent specific for the biological protecting group to form an unprotected terminal amino acid alpha-carbon reactive group. The unprotected terminal amino acid alpha-carbon reactive group is modified with a chemical modifying agent. The side chain protected terminally modified single copy polypeptide is then deprotected at the side chain groups to form a terminally modified recombinant single copy polypeptide. The number and sequence of steps in the method can be varied to achieve selective modification at the N- and/or C- terminal amino acid of the polypeptide.

Further preferred conjugates according to the invention consist of NK4 protein being covalently linked to a lower-alkoxy poly( ethylene glycol) group via a linker of the formula -C( O) -X-S-Y- with the C( O) of the linker forming an amide bond with an amino group of NK4 (as mentioned above, the ε-amino group of lysine residues is available), X is-(CH₂)_k- or -CH₂(O-CH₂-CH₂)_k-, k is from 1 to 10, Y is

the average molecular weight of the poly( ethylene glycol) moiety is from about 20 kDa to about 40 kDa and the molecular weight of the conjugate is from about 72 kDa to about 92 kDa at a molecular weight of 52 kDa for NK4 polypeptide, or from about 11 kDa to about 97 kDa at a molecular weight of 57 kDa for NK4 glyeoprotein.

This NK4 species may also be represented by formula (III)

P-NH-CO-X-S-Y-(OCH₂CH₂)_m-OR (HI)

wherein R may be any lower alkyl, by which is meant a linear or branched alkyl group having from one to six carbon atoms such as methyl, ethyl, isopropyl, etc. A preferred alkyl is methyl. X ma be -(CH₂)k- or -CH2(O-CH₂-CH₂)k-, wherein k is from 1 to about 10. Preferably, k is from 1 to about 4, more preferably, k is 1 or 2. Most preferably, X is -(CH₂).

In formula III, Y is

preferably

more preferably

In formula (III), the number m is selected such that the resulting conjugate of formula (III) has a physiological activity comparable to unmodified NK4, which activity may represent the same as, more than, or a fraction of the corresponding activity of unmodified NK4. m represents the number of ethylene oxide chains in the PEG unit. A single PEG subunit of -(OCH₂CH₂)- has a molecular weight of about 44 daltons. Thus, the molecular weight of the conjugate (excluding the molecular weight of the NK4) depends on the number m. A molecular weight of "about" a certain number means that it is within a reasonable range of that number as determined by conventional analytical techniques, m is therefore an integer ranging from about 450 to about 950 (corresponding to a molecular weight of from about 20 to 40 kDA).

Preferred NK4 proteins of formula (III) are represented by the formulae:

Most preferred NK4 protein products are represented by the formula:

These NK4 proteins may be prepared by

(a) covalently reacting a free amino group, preferably an ε-amino group of a lysine amino acid of an NK4 protein or the N-terminal amino group represented by the formula, P-NH₂, with a bi-functional reagent represented by the formula, Z-CO-X- S-Q, to form an intermediate with an amide linkage represented by the formula:

P-NH-CO-X-S-Q

wherein P is an NK4 protein less the amino group which forms an amide linkage; Z is a reactive group, e.g. a carboxylic-NHS ester; X is -(CH₂)_k- or -CH₂(O-CH₂-

CH₂)_k-. wherein k is from 1 to about 10; and Q is a protecting group, like alkanoyl, e.g. acetyl.

(b) covalently reacting the intermediate with an amide linkage from step (a) with an activated polyethylene glycol derivative represented by the formula, W- [OCH₂CH₂]_m-OR, to form an NK4 protein product represented by the formula:

wherein W is a sulfhydryl reactive form of Y; m is an integer ranging from about 450 to about 950; R is lower alkyl; and Y is as defined above.

In this embodiment, the bi-functional reagent is preferably N-succinimidyl-S- acetylthiopropionate or N-succinimidyl-S-acetylthioacetate, Z is preferably N- hydroxy-succinimide, and the activated polyethylene glycol derivative W- [OCH₂CH₂]_m-OR is preferably selected from the group consisting of iodo-acetyl- methoxy-PEG, methoxy-PEG-vinylsulfone, and methoxy-PEG-maleimide.

In more detail, the NK4 proteins of formula (III) may be prepared by covalent linking of a thiol group to NK4 ("activation") and coupling the resulting activated

NK4 with a poly(ethylene glycol) (PEG) derivative. The first step for the preparation of monoPEGylated NK4 according to the present invention comprises covalent linking of a thiol group via NH₂-groups of NK4. This activation of NK4 is performed with bi-functional reagents which carry a protected thiol group and an additional reactive group, such as active esters (e.g., a succinimidylester), anhydrides, esters of sulphonic acids, halogenides of carboxylic acids and sulphonic acids, respectively. The thiol group is protected by groups known in the art, e.g., acetyl groups. These bi-functional reagents are able to react with the amino groups by forming an amide linkage.

In a preferred embodiment the activation of the amino groups is performed by reaction with bi-functional reagents having a succinimidyl moiety. The bi- functional reagents may carry different spacer species, e.g. -(CH₂)_k- or -CH₂-(O- CH₂-CH₂-)_k- moieties, wherein k is from 1 to about 10, preferably from 1 to about 4, and more preferably 1 or 2, and most preferably 1. Examples of these reagents are N-succinimidyl-S-acetylthiopropionate (SATP) and N-succinimidyl-S- acetylthioacetate (SATA)

Acetylthioalkyl-carboxylic-NHS-ester, like

2-(Acetylthio)-(ethoxy)_k-acetic-acid-NHS-ester

with k as defined above.

The preparation of the bi-functional reagents is known in the art. Precursors of 2- (acetylthio)-(ethoxy)_k-acetic-acid-NHS-esters are described in DE-3924705, while the derivatization to the acetylthio compound is described by March, J., Advanced

Organic Chemistry (1977) 375-376. SATA is commercially available (Molecular Probes, Eugene, OR, USA and Pierce, Rockford, IL).

The addition of only one thiol group to an NK4 molecule can be selected by adjusting the reaction parameters, i.e., the protein (NK4) concentration and the protein/bi-functional reagent ratio.

The reaction is carried out, for example, in an aqueous buffer solution, pH 6.5-8.0, e.g., in 10 or 100 mM potassium phosphate, with or without 300 mM NaCl, pH 7.3. The bi-functional reagent may be added in DMSO. After completion of the reaction, preferably after 30 minutes, the reaction is stopped by addition of lysine. Excess bifunctional reagent may be separated by methods known in the art, e.g., by dialysis or column filtration. The average number of thiol groups added to NK4 can be determined by photometric methods described in, for example, Grasetti, D.R,. and Murray, J.F. in J. Appl. Biochem. Biotechnol. 119 (1967) 41-49.

The above reaction is followed by covalent coupling of an activated polyethylene glycol (PEG) derivative. Suitable PEG derivatives are activated PEG molecules with an average molecular weight of from about 20 to about 40 kDa.

Activated PEG derivatives are known in the art and are described in, for example, Morpurgo, M., et al. J. Bioconj. Chem. 7 (1996) 363 ff for PEG-vinylsulfone. Linear chain and branched chain PEG species are suitable for the preparation of the compounds of Formula 1. Examples of reactive PEG reagents are iodo-acetyl- methoxy-PEG and methoxy-PEG-vinylsulfone:

The use of these iodo-activated substances is known in the art and described e.g. by Hermanson, G.T., in Bioconjugate Techniques, Academic Press, San Diego (1996) p. 147-148.

Most preferably, the PEG species are activated by maleimide using (alkoxy-PEG- maleimide), such as methoxy-PEG-maleimide (MW 20,000 to 40,000; Shearwater Polymers, Inc.). The structure of alkoxy-PEG-maleimide is as follows:

with R and m are as defined above, preferably

The coupling reaction with alkoxy-PEG-maleimide takes place after in situ cleavage of the thiol protecting group in an aqueous buffer solution, e.g. 10 mM potassium phosphate, 300 mM NaCl, 2 mM EDTA, pH 6.2. The cleavage of the protecting group may be performed, for example, with hydroxylamine in DMSO at 25°C, pH 6.2 for about 90 minutes. For the PEG modification the molar ratio of activated NK4/alkoxy-PEG-maleimide should be from about 1:1 to about 1:6. The reaction may be stopped by addition of cysteine and reaction of the remaining thiol (-SH) groups with N-methylmaleimide or other appropriate compounds capable of forming disulfide bonds. Because of the reaction of any remaining active thiol groups with a protecting group such as N-methylmaleimide or other suitable protecting group, the NK4 proteins in the conjugates of this invention may contain such protecting groups. Generally the procedure described herein will produce a mixture of molecules having varying numbers of thiols protected by different numbers of the protecting group, depending on the number of activated thiol groups on the protein that were not conjugated to PEG-maleimide.

Whereas N-methylmaleimide forms the same type of covalent bond when used to block the remaining thiol-groups on the PEGylated protein, disulfide compounds will lead in an intermolecular sulfide/disulfide exchange reaction to a disulfide bridged coupling of the blocking reagent. Preferred blocking reagents for that type of blocking reaction are oxidized glutathione (GSSG), cysteine and cystamine. Whereas with cysteine no additional net charge is introduced into the PEGylated protein, the use of the blocking reagents GSSG or cystamine results in an additional negative or positive charge.

The further purification of the compounds of formula (III), including the separation of mono- from di-, tri- and multi-PEGylated NK4 species, maybe done by methods known in the art, e.g., column chromatography. The percentage of mono-PEG conjugates can be controlled by pooling broader fractions around the elution peak to decrease the percentage of mono -PEG or narrower fractions to increase the percentage of mono-PEG in the composition. About ninety percent mono-PEG conjugates is a good balance of yield and activity. Compositions in which, for example, at least ninety- two percent or at least ninety-six percent of the conjugates are mono-PEG species may be desired. In an embodiment of this invention the percentage of mono-PEG conjugates is from ninety percent to ninety- six percent.

Pharmaceutical formulations

The compounds of the present invention can be formulated according to methods for the preparation of pharmaceutical compositions which methods are known to the person skilled in the art. For the production of such compositions, monoPEGylated NK4 according to the invention is combined in a mixture with a pharmaceutically acceptable carrier. Such acceptable carriers are described, for example, in Remington's Pharmaceutical Sciences, 18^th edition, 1990, Mack Publishing Company, edited by Oslo et al. (e.g. pp. 1435-1712). Typical compositions contain an effective amount of the substance according to the invention, for example from about 0.1 to 100 mg/ml, together with a suitable amount of a carrier. The compositions maybe administered parenteraUy.

This invention further provides pharmaceutical compositions containing conjugates described herein in which the percentage of mono-PEG conjugates is preferably at least ninety percent, more preferably at least ninety-two percent.

The pharmaceutical formulations according to the invention can be prepared according to known methods in the art. Usually, solutions of monoPEGylated NK4 are dialyzed against the buffer intended to be used in the pharmaceutical composition and the desired final protein concentration is adjusted by concentration or dilution.

Such pharmaceutical compositions may be used for administration for injection and contain an effective amount of the monoPEGylated NK4 together with pharmaceutically acceptable diluents, preservatives, solubilizers, emulsifiers, adjuvants and/or carriers. Such compositions include diluents of various buffer contents (e.g. arginine, acetate, phosphate), pH and ionic strength, additives such as detergents and solubilizing agents (e.g. Tween 80/polysorbate, pluronic F68, sodium chloride, sodium sulfate), antioxidants (e.g. ascorbic acid, L-methionine), preservatives (Timersol, benzyl alcohol) and bulking substances (e.g. saccharose, mannitol), incorporation of the material into particulate preparations of polymeric compounds such as polylactic acid, polyglycolic acid, etc. or into liposomes. Such compositions may influence the physical state stability rate of release and clearance of the monoPEGylated NK4 according to the invention.

Dosages and drug concentrations

Typically, in a standard cancer treatment regimen, patients are treated with dosages in the range between 1 to 30 mg of monoPEGylated NK4 per kg per day over a certain period of time, lasting from one day to about 30 days or even longer. Drug is applied as a single daily subcutaneous or i.v. bolus injection of a pharmaceutical formulation containing 0.1 to 100 mg monoPEGylated NK4 per ml. This treatment can be combined with any standard (e.g. chemotherapeutic) treatment, by applying monoPEGylated NK4 before, during or after the standard treatment. This results in an improved outcome compared to standard treatment alone.

In any case the overall amount of administered PEGylated NK4 according to the invention is considerably lower than the amount of NK4 for the same treatment.

The following examples, references and the sequence listing are provided to aid the understanding of the present invention, the true scope of which is set forth in the appended claims. It is understood that modifications can be made in the procedures set forth without departing from the spirit of the invention.

Description of the Sequences

SEQ ID NO:l shows the DNA and polypeptide sequence of NK4. SEQ ID NO:2 shows the polypeptide sequence of NK4. Description of the Figures

Fig. 1 Inhibition of HGF-induced HUNEC proliferation by ΝK4,

20 kDa-mono-PEG-NK4 and 30 kDa-mono-PEG-NK4.

Fig. 2 Inhibition of HGF-induced HUVEC prohferation by NK4 and 40 kDa-mono-PEG-NK4.

Fig. 3 Inhibition of bFGF-induced HUVEC proliferation by NK4,

30 kDa-mono-PEG-NK4 and 40 kDa-mono-PEG-NK4.

Fig. 4 Time-concentration curve of NK4, 30 kDa-monoPEG-NK4,

40 kDa-mono-PEG-NK4 in plasma after i.v. (Fig. 4A) or s.c. (Fig. 4B) injection.

Example 1

Recombinant production of NK4

NK4 for therapeutic uses may be produced by recombinant means using bacterial or eukaryotic expression systems. Suitable eukaryotic expression systems are for example engineered HeLa, BHK or preferably CHO cells. Cells engineered for NK4 production are cultivated in a suitable medium. Typically, a 1 to 5 liter cell culture is used as inoculum for a 10 liter fermenter. After 3 to 5 days, the culture in the 10 liter fermenter can be used as inoculum for the 100 liter fermenter. After additional 3 to 5 days of fermentation, this culture can be used as inoculum for the 1000 liter production fermenter. After 3 to 4 days cells are removed by filtration or centrifugation and discarded. The NK4 containing supernatant is filtered, collected and processed during purification. The purification process is described in the following example.

Example 2 Purification

Heparin-Sepharose consists of Separose beads to the surface of which heparin is covalently bound. Since NK4 shows a high affinity to heparin it is retained on this column and can be eluted with high salt concentrations, whereas protein contaminants and other impurities either do not bind or elute at lower salt concentrations. NK4 containing fractions, eluting at about 0.7 to 1.1 M NaCl in 50 mM Hepes pH 7.5 are collected and loaded onto a hydroxyapatite column. NK4 elutes with about 0.4 to 0.7 M potassium phosphate, pH 7.5. The resulting fractions are substantially free of contaminating proteins and can be further purified by S- sepharose chromatography.

Example 3

Production of monoPEGylated NK4

NK4 purified in accordance with the above mentioned procedure was used for PEGylation reactions. Three of the above-mentioned suitable methods are exemplarily described.

a) PEGylation of NK4 with mPEG-SBA

Aliquots of NK4 were reacted with methoxy-PEG-SBA (5 kDa for comparison, 20 kDa and 30 kDa, respectively; Shearwater Polymers, Inc., Huntsville Alabama). Reaction was carried out at a protein to reagent ratio between 1:1 and 1:5 for about

2 h at room temperature (a ratio of 1:2 is preferred when using 20 and 30 kDa PEG). The reaction was stopped by the addition of 10 mM Tris-buffer or arginine HCl, pH 8, and samples were analyzed by SDS-PAGE or size exclusion chromatography on a Superose 6 column (Pharmacia) using as buffer solution 500 mmol/1 potassium phosphate, pH 6.8, for equilibration and elution. The reaction was optimized by varying protein to reagent ratio, pH, time and temperature, in order to obtain predominantly monoPEGylated NK4.

Such conditions are, for example:

Concentration NK4: 8-12 mg/ml Buffer system/pH: 0.3 M potassium phosphate, pH 8

Temperature: 25°C

Reaction time: 1 h

Molar ratios (protein:reagent): 1:2 Yield:

MonoPEGylated NK4: 38%

DiPEGylated NK4: 17%

UnPEGylated NK4: 45%

b) PEGylation of NK4 with mPEG-SPA

Aliquots of NK4 (protein concentration 8 to 12 mg/ml in 0.3 M potassium phosphate, pH 8) were reacted with methoxy- PEG-SPA (5 kDa for comparison and 20 kDa, respectively; Shearwater Polymers, Inc., Huntsville Alabama). Reaction was carried out at a protein to reagent ratio of 1:2 for about 2 h at room temperature.

The reaction was stopped by the addition of 10 mM Tris-buffer or arginine HCl and samples were analyzed by SDS-PAGE, reversed phase HPLC or size exclusion chromatography on a Superose 6 column (Pharmacia) using as buffer solution 500 mmol/1 potassium phosphate, pH 6.8, for equilibration and elution. The reaction was optimized by varying protein to reagent ratio, pH, time and temperature, in order to obtain predominantly monoPEGylated NK4, compared to di-and tri-PEGylated NK4.

c) PEGylation of NK4 with mPEG2-NHS

This PEGylation was performed as described in Example 3c with the exception that instead of PEG-SPA mPEG2-NHS (40 kDa PEG, branched via a lysine linker) was used at a molar ratio of 1:5 (protein:PEG reagent).

Example 4

Isolation of monoPEGylated NK4

MonoPEGylated NK4 can be separated from unPEGylated, di- and tri-PEGylated NK4 by running a preparative size exclusion chromatography (e.g. Superose 6 or

Superdex 200; Pharmacia) using as buffer solution 500 mmol/1 K-phosphate pH 6.8, for equilibration and elution, or by ion exchange chromatography. The purified protein contains predominantly the monoPEGylated species. Fractions were collected and analyzed by SDS-PAGE and reversed phase chromatography. Example 5

Molecular characterization of monoPEGylated NK4

a) Size exclusion chromatography

The monoPEGylated species elutes earlier in size exclusion chromatography (e.g. Superose 6 or Superdex 200; Pharmacia; using as buffer solution 500 mmol/1 K- phosphate pH 6.8, for equilibration and elution) as compared to the unmodified form. This is due to an increased hydrodynamic radius of the molecule.

b) SDS-PAGE

In SDS-PAGE proteins are separated according to their molecular weight. Due to an increase in molecular weight by PEGylation, the monoPEGylated NK4 shows a shorter migration distance as compared to the unmodified NK4. The migration distance is inversely correlated with the chain length of the PEG moiety and the number of PEG groups attached per NK4 molecule.

c) Peptide mapping

Digestion of monoPEGylated NK4 with sequence-specific endo-proteinases (e.g.

LysC or trypsin) results in a characteristic peptide map. The resulting peptides can be separated by reversed phase chromatography and analyzed by mass spectrometry and/or N-terminal sequencing. This allows for a determination of the PEG-modified groups within the NK4 molecule.

d) Reversed phase chromatography

MonoPEGylated NK4 can also be characterized by reversed phase chromatography. PEGylation of NK4 results in a change in retention time as compared to unmodified NK4. Example 6

Comparison of monoPEGylated, unPEGylated and multi-PEGylated NK4

In this example, unPEGylated NK4, NK4 monoPEGylated with PEG 5 kDa, PEG 20 kDa, PEG 30 kDa, PEG 40 kDa and multiPEGylated NK4 (NK4 PEGylated with more than one PEG chain ) were used.

a) Scatter assay

MDCK cells were subconfluentiy grown in tissue culture plates. Cells were treated with HGF (10 ng/ml) or with combinations of HGF and NK4. In these experiments the HGF-induced cell scattering was inhibited by the addition of a 10 to 1000-fold molar excess of NK4, showing the functional activity of PEGylated NK4. It was found that the in vitro activity of monoPEGylated 5 kDa-PEG-NK4, 20 kDa-PEG- NK4, 30 kDa-PEG-NK4, and 40 kDa-PEG-NK4 is similar to unPEGylated NK4. It was also found that the addition of more than one PEG chain (20 to 40 kDa) results in a significant loss of in vitro activity (Table 1).

Table 1 Scores of the MDCK scatter assay

The relative potency of various PEGylated forms of NK4 in the inhibition of HGF (10 ng/ml)-induced scattering of MDCK cells was assayed. ++ means complete inhibition, + means inhibition, +/- means weak inhibition and - means no inhibition. b) HUNEC Proliferation assay

Inhibition of the mitogenic activity of HGF by ΝK4 was determined by measuring proliferation of HUNECs in culture as described in Nakamura, T., et al., Nature 342 (1989) 440-443. In these experiments the HGF-induced cell proliferation was inhibited by the addition of a 10 to 1000-fold molar excess of NK4, showing the functional activity of mo noPEGylated NK4 (Figs. 1 and 2).

Alternatively, the HUVEC Proliferation assay was performed by inhibition of the mitogenic activity of bFGF (basic fibroblastic growth factor) by NK4. Inhibition was determined by measuring proliferation of HUVECs in culture as described in Kuba, K., et al., Cancer Res. 60 (2000) 6737-6743. In these experiments the FGF- induced cell proliferation was inhibited by the addition of a 10 to 1000-fold molar excess of NK4, showing the functional activity of monoPEGylated NK4 (Fig. 3).

c) Invasion assay

In this assay the invasive potential of tumor cells is analyzed. The assay was done essentially as decribed in Albini, A., et al. (1987) using HT115 cells. Again, HGF- induced (10 ng/ml) cell invasion could be inhibited by a 10 to 1000-fold molar excess of PEGylated NK4, showing the functional activity of monoPEGylated NK4.

Example 7 Activity in vivo

Model: Pane Tul human pancreatic cancer orthotopic SCID mouse model

(Alves, F., et al., Pancreas 23 (2001) 227-235)

Treatment: After 8 days, one application daily of monoPEGylated NK4 over a period of 21 days.

Doses: 16 mg/kg/day

4 mg/kg/day 1 mg/kg/day Placebo Result: Treatment with monoPEGylated NK4 shows a dose-dependent suppression of primary tumor growth and metastasis, whereas no effect is seen in placebo treated groups.

Example 8

Pharmaceutical composition

Suitable pharmaceutical compositions are, for example:

1 to 30 mg/ml monoPEGylated NK4 150 mM NaCl 10 mM sodium phosphate, pH 7.2

1 to 30 mg/ml monoPEGylated NK4 150 mM NaCl

0.01% Tween 80 or Tween 20 or pluronic F68 10 mM sodium phosphate, pH 7.2

1 to 30 mg/ml monoPEGylated NK4 50 mM NaCl 3% mannitol 10 mM sodium phosphate, pH 7.2

1 to 30 mg/ml monoPEGylated NK4 50 mM NaCl 3% mannitol 0.01% Tween 80 or Tween 20 or pluronic F68

10 mM sodium phosphate, pH 7.2

The compositions are prepared in that monoPEGylated NK4 is dialyzed against the above mentioned buffer solution (with or without mannitol). The protein concentration is adjusted by concentration or dilution with the buffer solution.

Detergent and NaCl are added out of a 10% stock solution. Example 9

Pharmacokinetic analysis of NK4, 30 kDa-mono-PEG-NK4 and 40 kDa-mono-

PEG-NK4

Adult mice (4 per group) received single i.v. or s.c. bolus injections of NK4, 30 kDa- mono-PEG-NK4 or 40 kDa-mono-PEG-NK4 (4 mg/kg in 0.25 ml injection volume), respectively. At several time points blood samples were taken and analyzed for NK4, 30 kDa-mono-PEG-NK4 or 40 kDa-mono-PEG-NK4 content by ELISA. The time-concentration curves were calculated and are shown in Figure 4. These data show that 30 kDa-mono-PEG-NK4 and 40 kDa-mono-PEG-NK4 have significantly improved stability in vivo, resulting in significantly increased plasma half-lives compared to unmodified NK4.

List of References

Albini, A., et al., Cancer Res. 47 (1987) 3239-3245

Alves, F., et al., Pancreas 23 (2001) 227-235

Bailon, P., Bioconjugate Chem. 12 (2001) 195-202 Date, K., et al., FEBS Letters 420 (1997) 1-6

Date, K., et al., Oncogene 17 (1989) 3045-3054

EP 0 442 724

EP-A 0473 084

Francis, G.E., et al., Int. J. Hematol. 68 (1998) 1-18 Gaertner, H.F., and Offord, R.E., Bioconjugate Chem. 7 (1996) 38-44

Grasetti, D.R.., and Murray, J.F., J. Appl. Biochem. Biotechnol. 119 (1967) 41-49

Heinzerling, L., et al., Dermatol. 201 (2000) 154-157

Hermanson, G.T., et al., Bioconjugate Techniques, Academic Press, San Diego, (1996) 147-148 Kuba, K., et al., Cancer Res. 60 (2000) 6737-6743

Maiti, P.K., et al., Int. J. Cancer Suppl. 3 (1988) 17-22

March, J., Advanced Organic Chemistry (1977) 375-376

Mehvar, R., J. Pharm. Pharm. Sci. 3 (1) (2000) 125-136

Miyazawa, K., et al., Biochem. Biophys. Res. Comm. 163 (1989) 967-973 Monfardini, C, et al., Bioconjugate Chem. 6 (1995) 62-69

Morpurgo, M., et al., Bioconj. Chem. 7 (1996) 363-368

Nakamura, T., et al, Biochem. Biophys. Res. Commun. 22 (1984) 1450-1459

Nakamura, T., et al., Nature 342 (1989) 440-443

Okajima, A., et al., Eur. J. Biochem. 193 (1990) 375-381 Parr, C, et al., Int. J. Cancer 85 (2000) 563-570

Reddy, K.R., Ann. Pharmacotherapy 34 (2000) 915-923

Remington's Pharmaceutical Sciences, 18^th edition, 1990, Mack Publishing Company, edited by Oslo et al. (e.g. pp. 1435-1712)

Seki, T., et al., Biochem. and Biophys. Res. Comm. 172 (1990) 321-327 Stuart, K.A., et al., International Journal of Experimental Pathology 81 (2000) 17-30

Tashiro, K., et al., Proc. Natl. Acad. Sci. USA 87 (1990) 3200-3204

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Tsutsumi et al., Proc. Natl. Acad. Sci. USA 97 (2000) 8548-8553 Tsutsumi, Y., et al., J. Pharmacol. Exp. Ther. 278 (1996) 1006-1011 Uematsu, Y., et al., J. Pharm. Sciences 88 (1999) 131-135 U.S. Patent No. 5,672,662 US Patent No. 5,932,462 U.S. Patent No. 5,977,310 Weidner, K.M., et al., Proc. Natl. Acad. Sci. USA 88 (1991) 7001-7005

WO 01/02017 WO 90/07938 WO 93/23541 WO 94/01451 WO 94/13322

Claims

Patent Claims

1. A conjugate comprising an N-terminal fragment of hepatocyte growth factor (HGF/SF) consisting of the hairpin domain and the four kringle regions of the α-chain and one polyethylene glycol group having a molecular weight of from about 20 to 40 kDa.

2. A conjugate according to claim 1, characterized in that said polyethylene glycol group has the formula

- CO - (CH₂)χ- (OCH₂CH₂)_mOR

and said -CO group forms an amide bond with one of the amino groups of said N-terminal fragment of hepatocyte growth factor, wherein

X is 2 or 3; m is from about 450 to about 950; R is (C,- )alkyl.

3. A conjugate according to claim 1, characterized in that said polyethylene glycol group has the formula

and said -CO group forms an amide bond with one of the amino groups of said N-terminal fragment of the hepatocyte growth factor, wherein yis l to lO; n and p together are from about 450 to 950; and R is (d-C₆)alkyl.

4. A conjugate according to claims 1 to 3, characterized in that the polyethylene glycol group has a molecular weight of from about 30 to 40 kDa.

5. A conjugate according to claims 1 to 4, characterized in that said polyethylene glycol group(s) is/are (a) monomethoxy polyethylene glycol group(s).

6. A pharmaceutical composition comprising a conjugate of claims 1 to 5 and a pharmaceutically acceptable carrier.

7. A process for preparing a pharmaceutical composition according to claim 6.

8. Use of a conjugate according to claims 1 to 5 for the preparation of a medicament useful in the treatment of cancer.

9. Method for the treatment of cancer diseases, comprising the steps of administering to a patient in need thereof a pharmaceutical composition according to claim 6.