AU2002232603A1 - Method for chemical transformation using a mutated enzyme - Google Patents

Method for chemical transformation using a mutated enzyme

Info

Publication number
AU2002232603A1
AU2002232603A1 AU2002232603A AU3260302A AU2002232603A1 AU 2002232603 A1 AU2002232603 A1 AU 2002232603A1 AU 2002232603 A AU2002232603 A AU 2002232603A AU 3260302 A AU3260302 A AU 3260302A AU 2002232603 A1 AU2002232603 A1 AU 2002232603A1
Authority
AU
Australia
Prior art keywords
chemical transformation
mutated enzyme
mutated
enzyme
transformation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002232603A
Inventor
J. David Rozzell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biocatalytics Inc
Original Assignee
Biocatalytics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocatalytics Inc filed Critical Biocatalytics Inc
Publication of AU2002232603A1 publication Critical patent/AU2002232603A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C12N9/0018—Phenylalanine dehydrogenase (1.4.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01009—Leucine dehydrogenase (1.4.1.9)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2002232603A 2000-10-31 2001-10-30 Method for chemical transformation using a mutated enzyme Abandoned AU2002232603A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US70242100A 2000-10-31 2000-10-31
US09/702,421 2000-10-31
US28837801P 2001-05-03 2001-05-03
US60/288,378 2001-05-03
US10/039,952 US7202070B2 (en) 2000-10-31 2001-10-24 Method for reductive amination of a ketone using a mutated enzyme
US10/039,952 2001-10-24
PCT/US2001/048577 WO2002036742A2 (en) 2000-10-31 2001-10-30 Method for chemical transformation using a mutated enzyme

Publications (1)

Publication Number Publication Date
AU2002232603A1 true AU2002232603A1 (en) 2002-05-15

Family

ID=27365635

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002232603A Abandoned AU2002232603A1 (en) 2000-10-31 2001-10-30 Method for chemical transformation using a mutated enzyme

Country Status (3)

Country Link
US (2) US7202070B2 (en)
AU (1) AU2002232603A1 (en)
WO (1) WO2002036742A2 (en)

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US7202070B2 (en) * 2000-10-31 2007-04-10 Biocatalytics, Inc. Method for reductive amination of a ketone using a mutated enzyme
CN101528917B (en) 2006-10-02 2015-07-29 科德克希思公司 The Statins pure for the preparation of stereoisomerism and the composition of synthetic intermediate thereof and method
JP2010517574A (en) * 2007-02-08 2010-05-27 コデクシス， インコーポレイテッド Ketreductase and uses thereof
US7977078B2 (en) 2007-08-24 2011-07-12 Codexis, Inc. Ketoreductase polypeptides for the production of (R)-3-hydroxythiolane
US8748143B2 (en) 2007-09-13 2014-06-10 Codexis, Inc. Ketoreductase polypeptides for the reduction of acetophenones
CN101889081B (en) * 2007-09-28 2014-06-18 科德克希思公司 Ketoreductase polypeptides and uses thereof
JP5646328B2 (en) * 2007-10-01 2014-12-24 コデクシス， インコーポレイテッド Ketreductase polypeptide for the production of azetidinone
WO2010025238A2 (en) 2008-08-27 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
US8288141B2 (en) * 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
CN102186972B (en) 2008-08-29 2014-08-20 科德克希思公司 Ketoreductase polypeptides for the stereoselective production of (4s)-3[(5s)-5(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one
CN102597226B (en) * 2009-06-22 2015-08-19 科德克希思公司 Transamination reaction
DK3409765T3 (en) * 2009-06-22 2021-09-27 Codexis Inc KETOREDUCTASE-MEDIATED STEREOSELECTIVE ROUTE FOR ALPHA CHLORAL ALCOHOLS
ES2575560T3 (en) 2009-08-19 2016-06-29 Codexis, Inc. Cetorreductase polypeptides to prepare phenylephrine
US9040262B2 (en) 2010-05-04 2015-05-26 Codexis, Inc. Biocatalysts for ezetimibe synthesis
HUE036947T2 (en) 2012-05-11 2018-08-28 Codexis Inc Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
DE102012017026A1 (en) 2012-08-28 2014-03-06 Forschungszentrum Jülich GmbH Sensor for NADP (H) and development of alcohol dehydrogenases
CN104513839B (en) * 2013-09-30 2017-12-26 中国科学院天津工业生物技术研究所 A kind of biocatalysis preparation method of D Terleus
EP2963121A1 (en) * 2014-07-03 2016-01-06 Basf Se Redox self-sufficient biocatalytic amination of alcohols
CN106906190B (en) * 2015-12-23 2019-10-18 中国科学院微生物研究所 One group of leucine dehydrogenase and its encoding gene and application
US9834802B2 (en) 2016-03-02 2017-12-05 Agrimetis, Llc Methods for making L-glufosinate
JP7022819B2 (en) 2017-11-06 2022-02-18 アシムケム ライフ サイエンス （ティエンジン） カンパニー リミテッド Transaminase mutants and their use
CN109609474B (en) * 2018-12-28 2020-07-28 浙江工业大学 Amino acid dehydrogenase mutant and application thereof in synthesis of L-glufosinate-ammonium
CN112394050A (en) * 2019-08-19 2021-02-23 中国科学院天津工业生物技术研究所 Detection method for high-throughput screening of ketone compounds and application of detection method in enzyme screening
CN111593077B (en) * 2019-12-30 2021-10-01 南京朗恩生物科技有限公司 Method for preparing (R) -4-chloro-3-hydroxy ethyl butyrate through biocatalysis
CN113583988B (en) * 2020-04-30 2023-09-12 沈阳药科大学 Amino acid dehydrogenase mutant and application thereof
CN114181918B (en) * 2020-12-04 2023-09-15 浙江科技学院 Omega-aminotransferase mutant obtained by DNA synthesis shuffling combination mutation and application

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US4710467A (en) 1983-07-29 1987-12-01 Purification Engineering, Inc. Process for preparing phenylalanine
US4518692A (en) 1983-09-01 1985-05-21 Genetics Institute, Inc. Production of L-amino acids by transamination
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US6544764B2 (en) * 2000-11-14 2003-04-08 Applera Corporation Isolated human dehydrogenase proteins, nucleic acid molecules encoding these human dehydrogenase proteins, and uses thereof

Also Published As

Publication number Publication date
US7202070B2 (en) 2007-04-10
US20020061564A1 (en) 2002-05-23
US20080076162A1 (en) 2008-03-27
WO2002036742A3 (en) 2003-08-21
WO2002036742A2 (en) 2002-05-10
US7642073B2 (en) 2010-01-05

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