AU2002232443A1 - Method for purifying adenoviruses - Google Patents

Method for purifying adenoviruses

Info

Publication number
AU2002232443A1
AU2002232443A1 AU2002232443A AU3244302A AU2002232443A1 AU 2002232443 A1 AU2002232443 A1 AU 2002232443A1 AU 2002232443 A AU2002232443 A AU 2002232443A AU 3244302 A AU3244302 A AU 3244302A AU 2002232443 A1 AU2002232443 A1 AU 2002232443A1
Authority
AU
Australia
Prior art keywords
adenovirus
hydroxyapatite
purifying adenoviruses
adenoviruses
purifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002232443A
Inventor
Susan V. Cannon-Carlson
Collette M. Cutler
Gary J. Vellekamp
Marcio Voloch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Sharp and Dohme Corp
Original Assignee
Schering Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Schering Corp filed Critical Schering Corp
Publication of AU2002232443A1 publication Critical patent/AU2002232443A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4746—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used p53
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material

Abstract

Methods for purifying adenoviruses from a contaminated sample using a hydroxyapatite medium are provided. Sodium chloride concentrations present in buffers used throughout the method are at least 150 mM to prevent the adenovirus from irreversibly binding to the hydroxyapatite. Levels of contaminants and empty adenovirus capsids from samples purified by conventional purification techniques are further reduced to provide a highly pure adenovirus preparation.
AU2002232443A 2000-11-29 2001-11-28 Method for purifying adenoviruses Abandoned AU2002232443A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25382300P 2000-11-29 2000-11-29
US60/253,823 2000-11-29
PCT/US2001/044684 WO2002044348A2 (en) 2000-11-29 2001-11-28 Method for purifying adenoviruses

Publications (1)

Publication Number Publication Date
AU2002232443A1 true AU2002232443A1 (en) 2002-06-11

Family

ID=22961846

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002232443A Abandoned AU2002232443A1 (en) 2000-11-29 2001-11-28 Method for purifying adenoviruses

Country Status (7)

Country Link
US (2) US20020064860A1 (en)
EP (1) EP1337627B1 (en)
AT (1) ATE455168T1 (en)
AU (1) AU2002232443A1 (en)
DE (1) DE60141081D1 (en)
ES (1) ES2337768T3 (en)
WO (1) WO2002044348A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004538005A (en) 2001-08-08 2004-12-24 ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア Method for purifying a viral vector having a protein binding to sialic acid
DK1780269T3 (en) 2004-02-23 2009-10-12 Crucell Holland Bv Virus Purification Methods
WO2006065827A2 (en) * 2004-12-13 2006-06-22 Canji, Inc. Cell lines for production of replication-defective adenovirus
DE602006003420D1 (en) 2005-04-11 2008-12-11 Crucell Holland Bv VIRUS CLEANING WITH ULTRA FILTRATION
EP1736538A1 (en) * 2005-06-21 2006-12-27 Cytos Biotechnology AG Process for the preparative purification of virus-like-particles (VLPs)
DE602006019916D1 (en) * 2005-12-12 2011-03-10 Canji Inc ADENOVIRAL EXPRESSION VECTORS WITH AN EXPRESSION CASSETTE IN THE E1 AREA AND AN INACTIVATED E2B POLYMERASE
WO2008060356A2 (en) * 2006-09-29 2008-05-22 Canji, Inc. Methods and compositions for gene therapy
ES2337973B8 (en) 2008-05-16 2011-07-21 Proyecto De Biomedicina Cima, S.L. ADENOVIRUS AUXILIARY SELF-INACTIVANTS FOR THE PRODUCTION OF ADENOVIRUS RECOMBINANTS OF HIGH CAPACITY.
ES2330826B1 (en) 2008-06-04 2010-07-26 Proyecto De Biomedicina Cima, S.L. HIGH CAPACITY ADENOVIRUS PACKING SYSTEM.
KR101504392B1 (en) 2008-11-03 2015-03-19 크루셀 홀란드 비.브이. Method for the production of adenoviral vectors
JP2011097918A (en) * 2009-10-07 2011-05-19 Hoya Corp Purification method and method for producing vaccine
CN102791852B (en) 2009-10-15 2014-05-07 克鲁塞尔荷兰公司 Method for the purification of adenovirus particles
EP2488635B1 (en) 2009-10-15 2013-11-20 Crucell Holland B.V. Process for adenovirus purification from high cell density cultures
AU2011214262B2 (en) 2010-02-15 2015-05-21 Crucell Holland B.V. Method for the production of Ad26 adenoviral vectors
SG11201900201YA (en) 2016-08-16 2019-02-27 Regeneron Pharma Methods for quantitating individual antibodies from a mixture
SG11201902667UA (en) 2016-10-25 2019-05-30 Regeneron Pharma Methods and systems for chromatography data analysis
TW202005694A (en) 2018-07-02 2020-02-01 美商里珍納龍藥品有限公司 Systems and methods for preparing a polypeptide from a mixture
WO2021255835A1 (en) * 2020-06-16 2021-12-23 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 Virus purification method using apatite column

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2507339B2 (en) * 1986-08-11 1996-06-12 三井東圧化学株式会社 Purification method of crude tPA
US5496926A (en) * 1992-01-19 1996-03-05 Yeda Research And Development Co. Ltd. Process of preparing a soluble LDL receptor
JPH07173189A (en) 1993-12-17 1995-07-11 Masashi Kosakai Method for using hydroxyapatite chromatography in two stages in presence of ligand
JPH08188599A (en) 1995-01-09 1996-07-23 Toagosei Co Ltd Separation and purification of immunoglobulin y antibody
US5837520A (en) * 1995-03-07 1998-11-17 Canji, Inc. Method of purification of viral vectors
CA2625279A1 (en) * 1995-08-30 1997-03-06 Genzyme Corporation Chromatographic purification of adenovirus and aav
JPH09169794A (en) 1995-12-22 1997-06-30 Mitsubishi Materials Corp Separation of proteins
EP0968284B1 (en) * 1996-11-20 2006-12-13 Introgen Therapeutics, Inc. An improved method for the production and purification of adenoviral vectors
US7732129B1 (en) * 1998-12-01 2010-06-08 Crucell Holland B.V. Method for the production and purification of adenoviral vectors
US6261823B1 (en) * 1996-12-13 2001-07-17 Schering Corporation Methods for purifying viruses
ID28298A (en) 1998-02-17 2001-05-10 Schering Corp COMPOSITION THAT CONTAIN VIRUSES AND METHODS TO ADAPT VIRUS PREPARATES
US6689600B1 (en) * 1998-11-16 2004-02-10 Introgen Therapeutics, Inc. Formulation of adenovirus for gene therapy
JP2000262280A (en) 1999-03-18 2000-09-26 Asahi Optical Co Ltd Purification of virus or viral antigen and prodution of vaccine

Also Published As

Publication number Publication date
ATE455168T1 (en) 2010-01-15
DE60141081D1 (en) 2010-03-04
ES2337768T3 (en) 2010-04-29
WO2002044348A3 (en) 2002-11-07
US7393631B2 (en) 2008-07-01
US20020064860A1 (en) 2002-05-30
US20060105456A1 (en) 2006-05-18
EP1337627A2 (en) 2003-08-27
WO2002044348A2 (en) 2002-06-06
EP1337627B1 (en) 2010-01-13

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