AU2002230979A1 - Fractionation of macro-molecules using asymmetric pulsed field electrophoresis - Google Patents

Fractionation of macro-molecules using asymmetric pulsed field electrophoresis

Info

Publication number
AU2002230979A1
AU2002230979A1 AU2002230979A AU3097902A AU2002230979A1 AU 2002230979 A1 AU2002230979 A1 AU 2002230979A1 AU 2002230979 A AU2002230979 A AU 2002230979A AU 3097902 A AU3097902 A AU 3097902A AU 2002230979 A1 AU2002230979 A1 AU 2002230979A1
Authority
AU
Australia
Prior art keywords
fractionation
macro
molecules
pulsed field
field electrophoresis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002230979A
Inventor
Robert Hamilton Austin
Lotien Richard Huang
James Christopher Sturm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Princeton University
Original Assignee
Princeton University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Princeton University filed Critical Princeton University
Publication of AU2002230979A1 publication Critical patent/AU2002230979A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
AU2002230979A 2000-12-18 2001-12-18 Fractionation of macro-molecules using asymmetric pulsed field electrophoresis Abandoned AU2002230979A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25629800P 2000-12-18 2000-12-18
US60256298 2000-12-18
PCT/US2001/048881 WO2002050095A1 (en) 2000-12-18 2001-12-18 Fractionation of macro-molecules using asymmetric pulsed field electrophoresis

Publications (1)

Publication Number Publication Date
AU2002230979A1 true AU2002230979A1 (en) 2002-07-01

Family

ID=22971713

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002230979A Abandoned AU2002230979A1 (en) 2000-12-18 2001-12-18 Fractionation of macro-molecules using asymmetric pulsed field electrophoresis

Country Status (5)

Country Link
US (3) US6881317B2 (en)
EP (1) EP1343800A4 (en)
JP (1) JP2004518663A (en)
AU (1) AU2002230979A1 (en)
WO (1) WO2002050095A1 (en)

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US7597791B2 (en) * 2001-10-19 2009-10-06 The Trustees Of Princeton University Method and apparatus for generating electric fields and flow distributions for rapidly separating molecules
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US8518228B2 (en) 2011-05-20 2013-08-27 The University Of British Columbia Systems and methods for enhanced SCODA
US20070090026A1 (en) * 2005-10-06 2007-04-26 Jongyoon Han Continuous biomolecule separation in a nanofilter
AU2006335194B2 (en) * 2006-01-03 2011-10-27 Alcon Inc. System for dissociation and removal of proteinaceous tissue
US8579117B2 (en) * 2008-07-24 2013-11-12 The Trustees Of Princeton University Bump array device having asymmetric gaps for segregation of particles
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US20110118734A1 (en) * 2009-11-16 2011-05-19 Alcon Research, Ltd. Capsularhexis device using pulsed electric fields
US20110135626A1 (en) * 2009-12-08 2011-06-09 Alcon Research, Ltd. Localized Chemical Lysis of Ocular Tissue
US20110144562A1 (en) * 2009-12-14 2011-06-16 Alcon Research, Ltd. Localized Pharmacological Treatment of Ocular Tissue Using High-Intensity Pulsed Electrical Fields
US20110144638A1 (en) * 2009-12-14 2011-06-16 Alcon Research, Ltd. Localized Shockwave-Induced Tissue Disruption
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CN102140462B (en) * 2010-04-29 2013-06-12 苏州吉玛基因股份有限公司 Human miR-1260 antisense nucleic acid and application thereof
US8546979B2 (en) 2010-08-11 2013-10-01 Alcon Research, Ltd. Self-matching pulse generator with adjustable pulse width and pulse frequency
CN113512522A (en) 2013-03-15 2021-10-19 普林斯顿大学理事会 Method and apparatus for high throughput purification
US20150064153A1 (en) 2013-03-15 2015-03-05 The Trustees Of Princeton University High efficiency microfluidic purification of stem cells to improve transplants
CN105264127B (en) 2013-03-15 2019-04-09 Gpb科学有限责任公司 The on piece microfluidic process of particle
EP3174976B1 (en) 2014-08-01 2020-10-28 GPB Scientific, Inc. Methods and systems for processing particles
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
US10976232B2 (en) 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration
US10850276B2 (en) 2017-02-24 2020-12-01 VisuGen Global LLC Systems and methods for capture and detection of low copy targets from large sample volumes
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US7597791B2 (en) * 2001-10-19 2009-10-06 The Trustees Of Princeton University Method and apparatus for generating electric fields and flow distributions for rapidly separating molecules

Also Published As

Publication number Publication date
WO2002050095A1 (en) 2002-06-27
US20020098504A1 (en) 2002-07-25
JP2004518663A (en) 2004-06-24
EP1343800A4 (en) 2005-06-29
US6881317B2 (en) 2005-04-19
EP1343800A1 (en) 2003-09-17
US20050161331A1 (en) 2005-07-28
US20090014332A1 (en) 2009-01-15

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