AU2002221989A1 - Method and medium for detecting/identifying micro-organisms - Google Patents
Method and medium for detecting/identifying micro-organismsInfo
- Publication number
- AU2002221989A1 AU2002221989A1 AU2002221989A AU2198902A AU2002221989A1 AU 2002221989 A1 AU2002221989 A1 AU 2002221989A1 AU 2002221989 A AU2002221989 A AU 2002221989A AU 2198902 A AU2198902 A AU 2198902A AU 2002221989 A1 AU2002221989 A1 AU 2002221989A1
- Authority
- AU
- Australia
- Prior art keywords
- medium
- solvent
- fatty acid
- organisms
- detecting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- G01N2333/255—Salmonella (G)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2496/00—Reference solutions for assays of biological material
- G01N2496/25—Reference solutions for assays of biological material containing added polymers to stabilise biological material against degradation or mantain viscosity or density, e.g. gelatin, polyacrylamides, polyvinyl alcohol
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A medium for biological analysis by biochemical means involving chromogenic or fluorogenic substrates that react with enzymes (esterases and/or osidases and/or peptidases and/or sulfatases and/or phosphatases) specific for the target strains is that is in a stable, ready-to-use liquid or gel form and contains at least one emulsifying stabilizer of the fatty acid sorbitan ester/fatty acid type and at least one solvent (dimethyl sulfoxide). This medium is obtained by mixing a stock solution of esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase substrates in the solvent, and the emulsifying stabilizer, with some of the constituents of the culture medium.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR0014879A FR2816956B1 (en) | 2000-11-17 | 2000-11-17 | METHOD AND MEDIUM FOR DETECTION / IDENTIFICATION OF BACTERIA WITH ESTERASIC ACTIVITY |
FR0014879 | 2000-11-17 | ||
PCT/FR2001/003611 WO2002040706A2 (en) | 2000-11-17 | 2001-11-16 | Method and medium for detecting/identifying micro-organisms |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2002221989A1 true AU2002221989A1 (en) | 2002-05-27 |
Family
ID=8856607
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2002221989A Abandoned AU2002221989A1 (en) | 2000-11-17 | 2001-11-16 | Method and medium for detecting/identifying micro-organisms |
Country Status (10)
Country | Link |
---|---|
US (1) | US7217536B2 (en) |
EP (1) | EP1334206B1 (en) |
JP (1) | JP3990279B2 (en) |
AT (1) | ATE327342T1 (en) |
AU (1) | AU2002221989A1 (en) |
BR (1) | BR0115435A (en) |
DE (1) | DE60119965T2 (en) |
ES (1) | ES2266309T3 (en) |
FR (1) | FR2816956B1 (en) |
WO (1) | WO2002040706A2 (en) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2802214B1 (en) * | 1999-12-09 | 2005-07-22 | Biomerieux Sa | NOVEL ALIZARIN CHROMOGENIC SUBSTRATES, USES THEREOF AND COMPOSITIONS CONTAINING SUCH SUBSTRATES |
GB0201039D0 (en) * | 2002-01-17 | 2002-03-06 | Incenta Ltd | Assay |
GB0201041D0 (en) * | 2002-01-17 | 2002-03-06 | Incenta Ltd | Assay |
EP1648286B1 (en) * | 2003-07-12 | 2017-12-20 | Accelerate Diagnostics, Inc. | Sensitive and rapid biodetection |
WO2005118884A1 (en) * | 2004-05-28 | 2005-12-15 | The United States Of America As Represented By The Secretary Of The Navy | A method for the rapid diagnosis of infectious disease by detection and quantitation of microorganism induced cytokines |
JP2006025608A (en) * | 2004-07-12 | 2006-02-02 | Chisso Corp | Microorganism culture medium |
FR2875241B1 (en) * | 2004-09-16 | 2010-07-30 | Biomerieux Sa | METHOD OF DETECTING STREPTOCOCCUS AGALACTIAE USING ESTERASE ACTIVITY |
JP2009000002A (en) * | 2007-06-19 | 2009-01-08 | Nissui Pharm Co Ltd | Culture medium for detecting microorganisms |
JPWO2009116127A1 (en) * | 2008-03-17 | 2011-07-21 | 尭 辻 | Screening method for efficiently obtaining useful microbial strains from microbial samples obtained from the natural environment, drugs used therefor and screening kit |
GB0820398D0 (en) | 2008-11-07 | 2008-12-17 | Oxoid Ltd | Semi-opaque medium for culture of microorganisms |
FR3000501B1 (en) | 2012-12-28 | 2015-08-14 | Biomerieux Sa | MICROORGANISM DETECTION MEDIUM COMPRISING AT LEAST ONE ALKYL (THIO) GLYCOSIDE |
FR3004195B1 (en) | 2013-04-03 | 2017-10-06 | Biomerieux Sa | USE OF AT LEAST ONE CHROMOGENIC AND / OR FLUOROGENIC PHOSPHATASE SUBSTRATE FOR DETECTION AND / OR ENTEROBACTERIUM DETECTION IN A BIOLOGICAL SAMPLE. |
FR3015522B1 (en) * | 2013-12-24 | 2017-02-24 | Biomerieux Sa | USE OF AT LEAST ONE CARBOXYLESTERASE AND / OR TRIACYLGLYCEROL-LIPASE SUBSTRATE FOR DETECTION OF BACILLUS CEREUS GROUP BACTERIA |
CN113155823A (en) * | 2021-05-21 | 2021-07-23 | 上海药明生物技术有限公司 | Method for characterizing degradation activity of esterase in host cell protein on polysorbate |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3878050A (en) * | 1974-04-01 | 1975-04-15 | Coors Co Adolph | Multi-differential agar culture medium |
US4144133A (en) * | 1977-08-25 | 1979-03-13 | J. K. And Susie L. Wadley Research Institute And Blood Bank | Fungal growth media |
US4717658A (en) * | 1985-12-03 | 1988-01-05 | Miles Inc. | Gram negative bacteria screening method with horseshoe crab amebocyte lysate (LAL) |
US4927927A (en) * | 1986-01-31 | 1990-05-22 | Eastman Kodak Company | Fluorescent dyes and biological and analytical uses thereof |
US4925789A (en) * | 1986-06-30 | 1990-05-15 | Edberg Stephen C | Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material |
US4994376A (en) * | 1987-05-27 | 1991-02-19 | The Research Foundation Of State University Of Ny | Detection of bacteroides gingivalis |
US4906573A (en) * | 1987-07-28 | 1990-03-06 | Miller Brewing Company | Culture medium for detection of beer spoilage microorganisms |
US5296370A (en) * | 1990-10-04 | 1994-03-22 | Rutgers, The State University | Repair medium for the resuscitation of injured cells |
FR2684110B1 (en) | 1991-11-25 | 1995-08-11 | Bio Merieux | MICROBIOLOGICAL ANALYSIS METHOD AND MEDIUM FOR DETECTING YEASTS OF THE CANDIDA ALBICANS SPECIES. |
US5534415A (en) * | 1991-11-25 | 1996-07-09 | Bio Merieux | Selective and differential medium for the growth and detection of Candida albicans |
FR2697028B1 (en) * | 1992-10-20 | 1995-01-06 | Alain Rambach | Culture medium for the detection of Salmonella and method for its use. |
FR2708286B1 (en) * | 1993-07-28 | 1995-10-20 | Rambach Alain | Method of identifying microorganisms with at least two chromogens. |
FI98379C (en) * | 1995-03-24 | 1997-06-10 | Orion Yhtymae Oy | Medium and method for identifying salmonella |
US5871944A (en) * | 1996-07-09 | 1999-02-16 | The University Of Maryland | Salmonellae preferential media |
GB2334100B (en) * | 1998-02-10 | 2000-03-22 | Praill Price Richardson Ltd | Chromogenic substrates, media, and methods for the detection of salmonella spp |
FR2777018B1 (en) * | 1998-04-02 | 2003-04-25 | Bio Merieux | NEW CHROMOGENEOUS SUBSTRATE AND METHOD OF USING THE SAME |
FR2816957B1 (en) * | 2000-11-17 | 2004-08-20 | Bio Merieux | METHOD AND MEDIUM FOR DETECTION / IDENTIFICATION OF MICROORGANISMS WITH ESTERASIC ACTIVITY |
-
2000
- 2000-11-17 FR FR0014879A patent/FR2816956B1/en not_active Expired - Lifetime
-
2001
- 2001-11-16 AU AU2002221989A patent/AU2002221989A1/en not_active Abandoned
- 2001-11-16 BR BR0115435-4A patent/BR0115435A/en not_active Application Discontinuation
- 2001-11-16 ES ES01996622T patent/ES2266309T3/en not_active Expired - Lifetime
- 2001-11-16 AT AT01996622T patent/ATE327342T1/en not_active IP Right Cessation
- 2001-11-16 US US10/416,591 patent/US7217536B2/en not_active Expired - Lifetime
- 2001-11-16 EP EP01996622A patent/EP1334206B1/en not_active Expired - Lifetime
- 2001-11-16 WO PCT/FR2001/003611 patent/WO2002040706A2/en active IP Right Grant
- 2001-11-16 JP JP2002543018A patent/JP3990279B2/en not_active Expired - Lifetime
- 2001-11-16 DE DE60119965T patent/DE60119965T2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE60119965T2 (en) | 2007-01-11 |
JP2004516827A (en) | 2004-06-10 |
WO2002040706A3 (en) | 2003-01-03 |
EP1334206A2 (en) | 2003-08-13 |
ES2266309T3 (en) | 2007-03-01 |
EP1334206B1 (en) | 2006-05-24 |
FR2816956B1 (en) | 2004-08-20 |
FR2816956A1 (en) | 2002-05-24 |
BR0115435A (en) | 2004-01-06 |
US7217536B2 (en) | 2007-05-15 |
US20050014215A1 (en) | 2005-01-20 |
WO2002040706A2 (en) | 2002-05-23 |
DE60119965D1 (en) | 2006-06-29 |
JP3990279B2 (en) | 2007-10-10 |
ATE327342T1 (en) | 2006-06-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2002221989A1 (en) | Method and medium for detecting/identifying micro-organisms | |
Wahlefeld | Triglycerides determination after enzymatic hydrolysis | |
Morı́s-Varas et al. | Visualization of enzyme-catalyzed reactions using pH indicators: rapid screening of hydrolase libraries and estimation of the enantioselectivity | |
Janes et al. | Quantitative screening of hydrolase libraries using pH indicators: identifying active and enantioselective hydrolases | |
US5306621A (en) | Enhanced chemiluminescent assay | |
CA1054907A (en) | Method and composition for blood serum cholesterol analysis | |
CN101023168A (en) | Reagent containing protease reaction promoter and/or colorant stabilizer | |
US3898130A (en) | Rapid enzymatic hydrolysis of triglycerides | |
Zornoza et al. | A protocol for the assay of arylesterase activity in soil | |
EP0489839A4 (en) | Novel hydrolytic enzyme inhibitors and substrates and assays, methods and kits embodying same | |
US5846754A (en) | Enzyme determination with protease inactivation | |
BRPI0016173A (en) | composition for detecting at least one strain and/or species of microorganisms and process for detecting the bacterial species pseudomonas aeruginosa | |
AU2002221988A1 (en) | Method and medium for detecting/identifying micro-organisms with esterase activity | |
JP3978622B2 (en) | Method for measuring components in body fluid and its reagent | |
Trautschold et al. | Kallikrein | |
Bigbee et al. | Sensitive fluorescence assays for urokinase using synthetic peptide 4-methoxy-β-naphthylamide substrates | |
JP2007306821A (en) | Composition for measuring activity of lipase and method for measuring activity | |
GB1590736A (en) | Chemical assay method | |
US20050009098A1 (en) | Process for generating the idiosyncratic catalytic imprint of a sample, the process of said imprint and the systems for implementation thereof | |
JP4244168B2 (en) | Analysis method and composition of ethanolamine-containing phospholipid | |
JPH01265899A (en) | Analyzing reagent and analyzing method for triglyceride | |
GB2237383A (en) | Enhanced chemiluminescent assay | |
Sierra | Hydrolysis of triglycerides by a bacterial proteolytic enzyme | |
Seitz et al. | A new staining method for the detection of activities of H2O2‐producing oxidases on gels and blots using cerium and 3, 3′‐diaminobenzidine | |
Meyer-Bertenrath | Lipolytic enzymes of the human pancreas |