AU2002220712A1 - Stem cells, method for expansion in vitro and use thereof - Google Patents

Stem cells, method for expansion in vitro and use thereof

Info

Publication number
AU2002220712A1
AU2002220712A1 AU2002220712A AU2071202A AU2002220712A1 AU 2002220712 A1 AU2002220712 A1 AU 2002220712A1 AU 2002220712 A AU2002220712 A AU 2002220712A AU 2071202 A AU2071202 A AU 2071202A AU 2002220712 A1 AU2002220712 A1 AU 2002220712A1
Authority
AU
Australia
Prior art keywords
stem cells
expansion
vitro
during
cultivation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002220712A
Inventor
William Rader
Klaus Rennebeck
Albert Scheller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10108264A external-priority patent/DE10108264A1/en
Application filed by Individual filed Critical Individual
Publication of AU2002220712A1 publication Critical patent/AU2002220712A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A method for expansion of stem cells in vitro is disclosed, whereby the stem cells are continuously or intermittently exposed to a micro-current and/or electromagnetic field of constant or alternating polarity during the expansion. Particular advantages of said invention arise from the fact that, during the in vitro cultivation, the stem cells may undergo a large number of dividing cycles without a differentiation in the stem cells occurring. Furthermore, no stoma cells are required during the cultivation. The invention further relates to the stem cells produced by the above method and the use thereof for the production of a medicament, in particular for the treatment of HIV.
AU2002220712A 2000-11-29 2001-11-16 Stem cells, method for expansion in vitro and use thereof Abandoned AU2002220712A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE10059346 2000-11-29
DE10059346.1 2000-11-29
DE10108264A DE10108264A1 (en) 2000-11-29 2001-02-21 Stem cells, methods for their expansion in vitro and their use
DE10108264.9 2001-02-21
PCT/EP2001/013329 WO2002044346A2 (en) 2000-11-29 2001-11-16 Stem cells, method for expansion in vitro and use thereof

Publications (1)

Publication Number Publication Date
AU2002220712A1 true AU2002220712A1 (en) 2002-06-11

Family

ID=26007839

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002220712A Abandoned AU2002220712A1 (en) 2000-11-29 2001-11-16 Stem cells, method for expansion in vitro and use thereof

Country Status (4)

Country Link
EP (1) EP1337625B1 (en)
AT (1) ATE271123T1 (en)
AU (1) AU2002220712A1 (en)
WO (1) WO2002044346A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100715366B1 (en) 2004-12-27 2007-05-07 김창현 Cell culture method
CN101166541A (en) * 2005-02-28 2008-04-23 雷格内泰克公司 Method and composition for repairing heart tissue
WO2006093881A2 (en) * 2005-02-28 2006-09-08 Regenetech, Inc. Method and composition for repairing epithelial and other cells and tissue
KR20070111533A (en) * 2005-02-28 2007-11-21 리제네텍 인코포레이티드 Method and composition for treating diabetes
JP5819861B2 (en) * 2011-01-19 2015-11-24 学校法人福岡大学 Method for suppressing the occurrence of apoptosis or necrosis

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0584184A4 (en) * 1991-04-09 1995-12-27 Indiana University Foundation System and process for supporting hematopoietic cells

Also Published As

Publication number Publication date
EP1337625B1 (en) 2004-07-14
WO2002044346A2 (en) 2002-06-06
EP1337625A2 (en) 2003-08-27
ATE271123T1 (en) 2004-07-15
WO2002044346A3 (en) 2002-07-25

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