AU2002220508A1 - Method for the identification of apoptotic and necrotic cells - Google Patents

Method for the identification of apoptotic and necrotic cells

Info

Publication number
AU2002220508A1
AU2002220508A1 AU2002220508A AU2050802A AU2002220508A1 AU 2002220508 A1 AU2002220508 A1 AU 2002220508A1 AU 2002220508 A AU2002220508 A AU 2002220508A AU 2050802 A AU2050802 A AU 2050802A AU 2002220508 A1 AU2002220508 A1 AU 2002220508A1
Authority
AU
Australia
Prior art keywords
apoptotic
necrotic cells
identification
cell suspension
lectin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002220508A
Inventor
Wolf Bertling
Udo Gaipl
Martin Herrmann
Petra Heyder
Joachim Robert Kalden
Christian Reiser
Walter Woith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Original Assignee
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin filed Critical November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Publication of AU2002220508A1 publication Critical patent/AU2002220508A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
    • G01N2333/42—Lectins, e.g. concanavalin, phytohaemagglutinin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2510/00—Detection of programmed cell death, i.e. apoptosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Physiology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

The invention relates to a method for the identification of apoptotic and necrotic cells in a cell suspension, comprising the following steps: a) contacting a first sample taken from said cell suspension with a lectin that is suitable to bind to sugar structures contained in the membrane of apoptotic and necrotic cells; b) acidifying the first sample; c) detecting the amount of lectin bound by the apoptotic and necrotic cells.
AU2002220508A 2000-10-27 2001-10-26 Method for the identification of apoptotic and necrotic cells Abandoned AU2002220508A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10053521A DE10053521B4 (en) 2000-10-27 2000-10-27 Method for the detection of apoptotic and necrotic cells
DE10053521.6 2000-10-27
PCT/DE2001/004028 WO2002035229A2 (en) 2000-10-27 2001-10-26 Method for the identification of apoptotic and necrotic cells

Publications (1)

Publication Number Publication Date
AU2002220508A1 true AU2002220508A1 (en) 2002-05-06

Family

ID=7661405

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002220508A Abandoned AU2002220508A1 (en) 2000-10-27 2001-10-26 Method for the identification of apoptotic and necrotic cells

Country Status (5)

Country Link
EP (1) EP1366362B1 (en)
AT (1) ATE310244T1 (en)
AU (1) AU2002220508A1 (en)
DE (2) DE10053521B4 (en)
WO (1) WO2002035229A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090053740A1 (en) * 2005-10-31 2009-02-26 Cedars-Sinai Medical Center Agglutination-based method for fast detection, isolation and quantification of apoptotic cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001504686A (en) * 1996-09-12 2001-04-10 ベーイーオー メリュー Method for detecting and / or quantifying gliotoxic factor

Also Published As

Publication number Publication date
EP1366362B1 (en) 2005-11-16
DE10053521A1 (en) 2002-05-08
DE50108118D1 (en) 2005-12-22
ATE310244T1 (en) 2005-12-15
WO2002035229A3 (en) 2003-10-09
EP1366362A2 (en) 2003-12-03
WO2002035229A2 (en) 2002-05-02
DE10053521B4 (en) 2004-07-08

Similar Documents

Publication Publication Date Title
WO2011062405A3 (en) Lateral flow immunoassay device
CA2588420E (en) Device and method for detection of analytes
WO2007035498A3 (en) Devices and methods for magnetic enrichment of cells and other particles
WO2001060517A3 (en) Container for nucleic acid analysis
WO2002077604A3 (en) Labeled cells for use as an internal functional control in rare cell detection assays
DE60331899D1 (en) Sensitives immunochromatografisches assay
WO2003018751A3 (en) Apparatus and method for electroporation of biological samples
WO2005021731A3 (en) Soluble analyte detection and amplification
ATE412049T1 (en) MULTIARRAY SYSTEM FOR INTEGRATION OF BIOARRAYS
WO2006080024A3 (en) Device, system and method for in vivo analysis
WO2005098439A3 (en) Lateral flow format, materials and mehtods
EP1092979A3 (en) Devices made by covalent bonding of molecules to an activated solid phase
WO2002094981A3 (en) Method of identifying peptides capable of binding to mhc molecules, peptides identified thereby and their uses
WO2006103439A3 (en) Cartridge for a fluid sample analyser
MY146468A (en) Devices and methods for detecting cells and other analytes
WO2001061043A3 (en) Parallel genotyping of multiple patient samples
WO2006119252A3 (en) Ultrathin nanoscale membranes, methods of making, and uses thereof
WO2006036738A3 (en) Photometric analysis
NZ571352A (en) Separation of nucleic acids from a liquid sample using a liquid flow device
WO2003046148A8 (en) Polypeptide quantitation
WO2003023051A3 (en) Lateral flow test format for enzyme assays
WO2008041004A3 (en) Preparation method
WO2007037910A3 (en) Specific removal of activated immune cells
WO2006053251A3 (en) Method of measuring amyloid-beta peptides
WO2009023331A3 (en) Antibodies and improved test sample handling methods for use in assays for myeloperoxidase