AU2002214569A1 - System and method for determining the presence of methylated cytosines in polynucleotides - Google Patents

System and method for determining the presence of methylated cytosines in polynucleotides

Info

Publication number
AU2002214569A1
AU2002214569A1 AU2002214569A AU1456902A AU2002214569A1 AU 2002214569 A1 AU2002214569 A1 AU 2002214569A1 AU 2002214569 A AU2002214569 A AU 2002214569A AU 1456902 A AU1456902 A AU 1456902A AU 2002214569 A1 AU2002214569 A1 AU 2002214569A1
Authority
AU
Australia
Prior art keywords
polynucleotides
determining
methylated cytosines
cytosines
methylated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002214569A
Inventor
Qingbo Li
Zhaowei Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spectrumedix Corp
Original Assignee
Spectrumedix Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spectrumedix Corp filed Critical Spectrumedix Corp
Publication of AU2002214569A1 publication Critical patent/AU2002214569A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
AU2002214569A 2000-10-11 2001-10-10 System and method for determining the presence of methylated cytosines in polynucleotides Abandoned AU2002214569A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23911900P 2000-10-11 2000-10-11
US60239119 2000-10-11
PCT/US2001/031665 WO2002031199A1 (en) 2000-10-11 2001-10-10 System and method for determining the presence of methylated cytosines in polynucleotides

Publications (1)

Publication Number Publication Date
AU2002214569A1 true AU2002214569A1 (en) 2002-04-22

Family

ID=22900694

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002214569A Abandoned AU2002214569A1 (en) 2000-10-11 2001-10-10 System and method for determining the presence of methylated cytosines in polynucleotides

Country Status (4)

Country Link
US (1) US20040048276A1 (en)
AU (1) AU2002214569A1 (en)
CA (1) CA2425413A1 (en)
WO (1) WO2002031199A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1320745A4 (en) 2000-09-01 2008-10-01 Applera Corp System and method for temperature gradient capillary electrophoresis
US6872530B2 (en) 2002-04-24 2005-03-29 Spectrumedix, Llc Method for determining the presence of DNA variants using peptide nucleic acid probes
US7303879B2 (en) 2003-07-31 2007-12-04 Applera Corporation Determination of SNP allelic frequencies using temperature gradient electrophoresis
US20080172183A1 (en) * 2006-02-10 2008-07-17 Achim Karger Systems and methods for methylation prediction
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
FR2957909B1 (en) 2010-03-29 2012-07-20 Rc Lux LIQUID DISPENSER AND METHOD OF OPERATING A DISPENSER OF A LIQUID
EP4010491A1 (en) * 2019-08-08 2022-06-15 Universität Zürich Probe-induced heteroduplex mobility assay

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3622591C2 (en) * 1986-07-04 1998-11-19 Qiagen Gmbh Method and device for producing a controllable and reproducible temperature gradient and its use
NL8801147A (en) * 1988-05-02 1989-12-01 Tno METHOD FOR DETECTING GENETIC VARIATION, AND SUITABLE KIT.
US5795720A (en) * 1989-08-19 1998-08-18 Qiagen Gmbh Process and device for the separation and detection of components of a mixture of materials by temperature gradient gel electrophoresis
US5935522A (en) * 1990-06-04 1999-08-10 University Of Utah Research Foundation On-line DNA analysis system with rapid thermal cycling
DE4234086A1 (en) * 1992-02-05 1993-08-12 Diagen Inst Molekularbio METHOD FOR DETERMINING NUCLEIC ACID SEQUENCES AMPLIFIED IN VITRO
US5736025A (en) * 1994-04-04 1998-04-07 Genomyx Inc. Control of temperature gradients during gel electrophoresis using turbulent gas flow
US6475721B2 (en) * 1995-03-04 2002-11-05 Boston Probes, Inc. Sequence specific detection of nucleic acids using a solid carrier bound with nucleic acid analog probes
AU5775696A (en) * 1995-06-06 1996-12-24 Beltronics Inc Automatic protein and/or dna analysis system and method
US20020012902A1 (en) * 1995-10-06 2002-01-31 Martin Fuchs Methods and kit for hybridization analysis using peptide nucleic acid probes
US5734058A (en) * 1995-11-09 1998-03-31 Biometric Imaging, Inc. Fluorescent DNA-Intercalating cyanine dyes including a positively charged benzothiazole substituent
US6613508B1 (en) * 1996-01-23 2003-09-02 Qiagen Genomics, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6265557B1 (en) * 1997-05-09 2001-07-24 Loma Linda University Medical Center ABO histo-blood group O alleles of the baboon
US5998147A (en) * 1997-08-15 1999-12-07 Institut Pasteur Mutated polynucleotide corresponding to a mutation responsible for prelingual non-syndromic deafness in the connexin 26 gene and method of detecting this hereditary defect
JP2002523776A (en) * 1998-08-31 2002-07-30 シー．ビー．エス．サイエンティフィック カンパニー，インコーポレイティド Two-dimensional gel electrophoresis system
WO2001068919A2 (en) * 2000-03-10 2001-09-20 Ana-Gen Technologies, Inc. Mutation detection using denaturing gradients
US6486309B1 (en) * 2001-08-01 2002-11-26 Varian, Inc. Method of detecting sequence variations in macromolecules by thermal gradiant
US6872530B2 (en) * 2002-04-24 2005-03-29 Spectrumedix, Llc Method for determining the presence of DNA variants using peptide nucleic acid probes

Also Published As

Publication number Publication date
CA2425413A1 (en) 2002-04-18
US20040048276A1 (en) 2004-03-11
WO2002031199A1 (en) 2002-04-18

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